NELFB cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	NELFB
Entrez Gene ID	106893945
Full Name	negative elongation factor complex member B
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014952303.1	XP_014807789.1	negative elongation factor B

Pathways from BioSystems
Gene Ontology

The following NELFB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NELFB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa72552	XM_014952303.1 Latest version!	Calidris pugnax negative elongation factor complex member B (NELFB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NELFB ( XM_014952303.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014807789.1 Calidris pugnax negative elongation factor complex member B (NELFB), mRNA.

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CloneID	OCa72552
Clone ID Related Accession (Same CDS sequence)	XM_014952303.1
Accession Version	XM_014952303.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	negative elongation factor B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090961.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACGCGG GGCTGCAGGA GCTGGGGGTG GCCAACGGCG AGGACCTGAA GGAGACGCTG 
ACCAACTGCA CGGAGCCGCT GAAGGCCATC GAGCAGTTCC AGACGGAAAA TGGAGTGCTC 
CTGCCCTCGC TGCAGTCGGC CCTGCCCTTC CTGGACCTGC ACGGCACCCC CCGGCTGGAG 
TTCCACCAGT CCGTGTTCGA CGAGCTGAGG GAGAAGCTGC TGGAGAGGGT CTCTGCCATC 
GCTTTGGAAG GGAAAGTCGA GGAGAGATAC AAGAAGCTGG AAGATCTCCT AGAGAAGAGC 
TTTTCCCTGG TCAAGATGCC TTCCATACAG CCTGTGGTGA TGTGTGTTAT GAAGCACTTG 
CCCAAGGTCC CTGAAAAGAA GCTGAAGTTA GTAATGGCCG ATAAGGATTT GTATAAAGCG 
TGTGCGGTGG AGGTGAAGCG CCAGATCTGG CAGGATAACC AGGCTCTGTT TGGTGATGAG 
GTGTCTCCGC TGCTGAAGCA GTATATCCTG GAGAAAGAAA ATATTCTCTT CAGCAACGAT 
ATTTCTGTCT TGCACAATTT CTTCAGTCCG TCTCCCAAAA CGAGACGTCA AGGAGAGGTG 
GTTCAGAAGC TGACCCAGAT GATCGGGAAG AACGTGAAGC TCTATGATAT GGTGTTGCAG 
TTTCTGAGAA CCTTGTTCCT TCGGACAAGG AATGTTCATT ACTGCACACT ACGGGCAGAG 
CTCCTGATGT CGCTGCATGA CCTGGAAATC AGTGAAATCT GCACCGTTGA CCCCTGTCAC 
AAGTTCACCT GGTGCCTTGA TGCTTGCATT CGGGAGAAGT TCGTGGACAA CAAGAGAGCT 
CGGGAACTGC AAGGGTTTCT GGATGGAGTG AAGAAGGGAC AGGAACAAGT GTTGGGGGAC 
TTATCGATGA TCTTGTGTGA TCCTTTTGCC ATTAACACCT TGGCCTTGAG CACCATCAGG 
AACCTACAGG ACCTGGTTGG GCAGGACACC TTACCCAGGG AAAGCCCAGA TCTGCTGCTG 
CTCCTTCGGA TGCTGTCTTT GGGACAGGGG GCCTGGGACA TGATTGACAG CCAAGTCTTC 
AAGGAACCAA AAATGGAAGC TGAGCTGATC ACGAAGTTCT TGCCTATGCT GATGTCCTTC 
GTGGTGGATG ACCACACGTT CAACGTAGAT CAGAAGCTGC CCTCGGAGGA GAAGGGACCA 
ATTCCTTACC CCAGCACCAT TCCTGAAGCT TTCACCAAAT TCTTGCAGGA GAACAGAATA 
GCTTGTGAGA TTGGGCTGTA TTACATCCTT CACATCACGA AACTGAGGAA CAAGAACGCT 
TTCCTGAGGC TCCTGCCAGC ACTAGTTGAG ACTTTCAGTG ACTTGGCCTT CAGTGATATT 
TTTCTGCATC TGCTCACTGG TAACCTCACG CTGCTGGGTG ATGAATTTGC ACTGGAGGAG 
TTCTGTACCA GTCTCTTTGA TGGCTTCTTT CTCACTGCCT GCTCAAGAAA GGAGAACGTA 
CACAGACATG TGCTAAGGCT GCTGCTTCAT CTGCATCACA AAGTGGCACC TGCCAAACTA 
GAGTCTCTCC AGAAGGCTTT GGAACCCACC AAGCAGAGCG GGGAGGCTGT GAAGGAGCTT 
TATAACCAGC TCACGGAGAA GCTGGAGCTT CGCAAGCCGA GTCCAGCCGA AGCGACTGAG 
ACTCCCTCCA TGGAGCTGCC CCTGCCCACG GTGCCCACCC CAGCCTCACG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014807789.1
CDS	54..1787
Translation	MYAGLQELGVANGEDLKETLTNCTEPLKAIEQFQTENGVLLPSLQSALPFLDLHGTPRLEFHQSVFDELREKLLERVSAIALEGKVEERYKKLEDLLEKSFSLVKMPSIQPVVMCVMKHLPKVPEKKLKLVMADKDLYKACAVEVKRQIWQDNQALFGDEVSPLLKQYILEKENILFSNDISVLHNFFSPSPKTRRQGEVVQKLTQMIGKNVKLYDMVLQFLRTLFLRTRNVHYCTLRAELLMSLHDLEISEICTVDPCHKFTWCLDACIREKFVDNKRARELQGFLDGVKKGQEQVLGDLSMILCDPFAINTLALSTIRNLQDLVGQDTLPRESPDLLLLLRMLSLGQGAWDMIDSQVFKEPKMEAELITKFLPMLMSFVVDDHTFNVDQKLPSEEKGPIPYPSTIPEAFTKFLQENRIACEIGLYYILHITKLRNKNAFLRLLPALVETFSDLAFSDIFLHLLTGNLTLLGDEFALEEFCTSLFDGFFLTACSRKENVHRHVLRLLLHLHHKVAPAKLESLQKALEPTKQSGEAVKELYNQLTEKLELRKPSPAEATETPSMELPLPTVPTPASR

Target ORF information:

RefSeq Version	XM_014952303.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax negative elongation factor complex member B (NELFB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014952303.1

ORF Insert Sequence:

ATGTACGCGG GGCTGCAGGA GCTGGGGGTG GCCAACGGCG AGGACCTGAA GGAGACGCTG 
ACCAACTGCA CGGAGCCGCT GAAGGCCATC GAGCAGTTCC AGACGGAAAA TGGAGTGCTC 
CTGCCCTCGC TGCAGTCGGC CCTGCCCTTC CTGGACCTGC ACGGCACCCC CCGGCTGGAG 
TTCCACCAGT CCGTGTTCGA CGAGCTGAGG GAGAAGCTGC TGGAGAGGGT CTCTGCCATC 
GCTTTGGAAG GGAAAGTCGA GGAGAGATAC AAGAAGCTGG AAGATCTCCT AGAGAAGAGC 
TTTTCCCTGG TCAAGATGCC TTCCATACAG CCTGTGGTGA TGTGTGTTAT GAAGCACTTG 
CCCAAGGTCC CTGAAAAGAA GCTGAAGTTA GTAATGGCCG ATAAGGATTT GTATAAAGCG 
TGTGCGGTGG AGGTGAAGCG CCAGATCTGG CAGGATAACC AGGCTCTGTT TGGTGATGAG 
GTGTCTCCGC TGCTGAAGCA GTATATCCTG GAGAAAGAAA ATATTCTCTT CAGCAACGAT 
ATTTCTGTCT TGCACAATTT CTTCAGTCCG TCTCCCAAAA CGAGACGTCA AGGAGAGGTG 
GTTCAGAAGC TGACCCAGAT GATCGGGAAG AACGTGAAGC TCTATGATAT GGTGTTGCAG 
TTTCTGAGAA CCTTGTTCCT TCGGACAAGG AATGTTCATT ACTGCACACT ACGGGCAGAG 
CTCCTGATGT CGCTGCATGA CCTGGAAATC AGTGAAATCT GCACCGTTGA CCCCTGTCAC 
AAGTTCACCT GGTGCCTTGA TGCTTGCATT CGGGAGAAGT TCGTGGACAA CAAGAGAGCT 
CGGGAACTGC AAGGGTTTCT GGATGGAGTG AAGAAGGGAC AGGAACAAGT GTTGGGGGAC 
TTATCGATGA TCTTGTGTGA TCCTTTTGCC ATTAACACCT TGGCCTTGAG CACCATCAGG 
AACCTACAGG ACCTGGTTGG GCAGGACACC TTACCCAGGG AAAGCCCAGA TCTGCTGCTG 
CTCCTTCGGA TGCTGTCTTT GGGACAGGGG GCCTGGGACA TGATTGACAG CCAAGTCTTC 
AAGGAACCAA AAATGGAAGC TGAGCTGATC ACGAAGTTCT TGCCTATGCT GATGTCCTTC 
GTGGTGGATG ACCACACGTT CAACGTAGAT CAGAAGCTGC CCTCGGAGGA GAAGGGACCA 
ATTCCTTACC CCAGCACCAT TCCTGAAGCT TTCACCAAAT TCTTGCAGGA GAACAGAATA 
GCTTGTGAGA TTGGGCTGTA TTACATCCTT CACATCACGA AACTGAGGAA CAAGAACGCT 
TTCCTGAGGC TCCTGCCAGC ACTAGTTGAG ACTTTCAGTG ACTTGGCCTT CAGTGATATT 
TTTCTGCATC TGCTCACTGG TAACCTCACG CTGCTGGGTG ATGAATTTGC ACTGGAGGAG 
TTCTGTACCA GTCTCTTTGA TGGCTTCTTT CTCACTGCCT GCTCAAGAAA GGAGAACGTA 
CACAGACATG TGCTAAGGCT GCTGCTTCAT CTGCATCACA AAGTGGCACC TGCCAAACTA 
GAGTCTCTCC AGAAGGCTTT GGAACCCACC AAGCAGAGCG GGGAGGCTGT GAAGGAGCTT 
TATAACCAGC TCACGGAGAA GCTGGAGCTT CGCAAGCCGA GTCCAGCCGA AGCGACTGAG 
ACTCCCTCCA TGGAGCTGCC CCTGCCCACG GTGCCCACCC CAGCCTCACG CTGA