NSMAF cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	NSMAF
Entrez Gene ID	106894133
Full Name	neutral sphingomyelinase activation associated factor
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014952645.1	XP_014808131.1	protein FAN

Pathways from BioSystems
Gene Ontology

The following NSMAF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NSMAF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa72798	XM_014952645.1 Latest version!	Calidris pugnax neutral sphingomyelinase activation associated factor (NSMAF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NSMAF ( XM_014952645.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014808131.1 Calidris pugnax neutral sphingomyelinase activation associated factor (NSMAF), mRNA.

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CloneID	OCa72798
Clone ID Related Accession (Same CDS sequence)	XM_014952645.1
Accession Version	XM_014952645.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	protein FAN
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090968.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGCGCTTTC TTAACGAGCA GGCTATTCAG TTTTCGCTGC TGCTTCTTAA CCTGGAAGAA 
TATTACTTTG AACAGCACAC AGCTAATCAT ATTATAAATA AAGGTTGCAG AGATGAAAGA 
AAATTCAGAG GCTCTCTGAA AATCTGTTCA AAGTCAATTA TTTTTGAACC TGATGACAAT 
ATCCAACCCA TTATCAAGAT ACCACTGAGA GACTGCGTTA GTATAAAAGC CCCAGAAGAC 
AATGAAGCAA GTAACCCTTT CACACGGGAT GCATCTGGAG GGATTTCTGT TGTATGTAAT 
CAGGTTTTTC TCATTAAAGA AAGAAACATT ATTGCACCCT ATAAAACAGT AAGAGGTAGA 
ACAGAACACT TATTCCAACT GGATGTTGCT GGGAAACTAG GAGACGTGGT GCAAACTCTG 
CATCAGCTTT ACAGGGCCTC TTGTCTAGAT AAGATGGGAG ATCAAGCTGC CATGATTACT 
GCTATATTGC AATCGCGCTT GGCTAGGACG TCATTTGATA AAAACAGATT TCAAAGCATT 
TCTGAAACGC TGCATATGGA ATGTAAAGCA GAAATGGTGA CACCACTGGT GACTAATCCA 
GGGCATGTGT GTGTTACTGA TGCTAACTTG TACTTCCAGC CTCTTAATGG ATATCCTAAA 
CCAGTAGTAC AAATAACACT GCAAAACGTG CGTCGCATCT ATAAAAGACG ACATGGTTTA 
ATGCCACTGG GACTTGAAGT GTTTTGCACA GAAAATGATC TATGTTCTGA CATCTACTTG 
AAATTCTACA ACTGTCAAGA TCGAGATGAG CTCTATTTCC TTATTGCAAC ATATATAGAA 
AATCATGTAA CAGAGCATAC AGCTGAAAGT TATATGCTGC AATGGCAGCG AGGACATATA 
TCAAACTACC AGTATCTTCT TCATCTCAAC AATCTGGCTG ACAGGAGCTG CAACGATCTC 
TCCCAGTATC CTGTGTTTCC GTGGATCATA GCAGACTACT CTAGTTCAGT ATTAGATTTG 
ACAAAGCCTG AAACATTCCG TGACCTTAGT AAACCAATTG GTGCCCTGAA TAAGGAAAGG 
CTGGATAGAT TATTGACACG TTACCAGGAA ATGCCAGAGC CGAAATTCAT GTATGGGAGC 
CATTATTCCT CTCCGGGTTA TGTTTTGTTT TATCTCGTCA GAGTGGCTCC GGAATACATG 
CTGTGTCTGC AGAACGGAAA GTTTGATCAC GCTGACAGAA TGTTCAACAG TCTTGCAGAA 
ACCTGGAAAA ACTGTTTGGA TGGTGCAACA GATTTCAAAG AGTTGATTCC AGAATTTTAT 
GAAAATGATT CTAGTTTTCT AGTAAACAGT TTGAAGTTGG ACTTGGGAAA AAGGCAGGGT 
GGGAAGATGG TTGAAGATGT AGAGCTTCCC CCTTGGGCAT CAGGTCCTGA TGACTTTCTT 
CAGAAGAGCC AAGAGGCTTT AGAAAGTCAG TATGTATCGG AACATCTTCA TGAATGGATT 
GACATCATAT TTGGCTACAA GCAGAAAGGA AGTGAAGCAG TTGAAGCTCA CAATGTATTT 
CACCCATTAA CTTATGAAGG AGGAGTGGAT TTAAACAGTA TTATGGACCC CAATGAAAAA 
GTAGCTCTGC TGACACAAAT CTTGGAGTTT GGACAGACGC CAAAGCAATT GTTTACAATT 
CCTCATCCCC GAAGGATCGT ACCAAAGCTG AAAAGCCTGT CTCGAACGTC GAGCCACAGT 
GTTTCCATGG CTGAGTCTCC AGTTTCTCCA AATGAAGAAT CATTTGAAGA TTTGACAGAA 
GAAAGTATAA CACTTGCTTG GAACAATATT GCCAAACTAA AATTAGATGA GCGATATAAA 
ATTCACAAAG AGGCAATTAC TGGAATAGCA GTCACCTGCA ATGGGTCTTC TGTTTTCACT 
ACATCCCAGG ATTCAACTTT GAAGATGTTT TCCAAAGAAT CAAAGACAAT CCAGAGAAGT 
GTGTCTTTTT CAAACATGGC TTTGTCATCT TGTCTAATCT TACCAGGAGA TACCACTGTA 
ATAAGTTCTT CATGGGACAA TCATATATAT TTTTATTCAA TTGCATTTGG AAGACAGCAA 
GACATCTTAA TGGGGCATGA TGATGCAGTC AGTAAGATCT GTTGGCGTGA TGGGCGCTTA 
TATTCTGCCT CTTGGGATTC CACTGTCAAG GTGTGGCGCT GTCTGCCCGG GGAGACCTCC 
AGCAACAAAA AACACCACTC GGAGCTGCTC GCAGAGTTGG AACACGACGT TAGTGTAAAC 
ACCATCGACC TTAACGCTGC AAATACTATG TTGGCATCTG GAACCAAAGA GGGTACCATC 
AGTGTCTGGG ATGTTCCTAC AGCAACAGTC TTGCACCAGC TGCCGTGTCA TTCTGGGACT 
GTGTTTGATG CTGCTTTCAG TCCTGACAGC AGACACCTAC TCAGCACCGG AGAGGATTGT 
TGTTTCAAAG TAATAGATGT ACAAACCGGA ATGCTTATAT CTTCTGTAAC TGCTGCTGAG 
CCACAGAGAT GCTTCAAATG GGATGGAAAT ACCATCTTAT CGGGAAGTCA ATCTGGTGAA 
TTAATGATTT GGGACCTTCT TGGAGGAAAA GTTATGGAAA GAATCAAAGG ACATACAGGT 
GCTGTAACAT CCATGTGGAT GAACGAACAG TGCAACAGTG TTATTACAGG AGGGGAAGAC 
AAACAAATCA TGTTCTGGAA ACTGCAGTAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014808131.1
CDS	1..2733
Translation	MRFLNEQAIQFSLLLLNLEEYYFEQHTANHIINKGCRDERKFRGSLKICSKSIIFEPDDNIQPIIKIPLRDCVSIKAPEDNEASNPFTRDASGGISVVCNQVFLIKERNIIAPYKTVRGRTEHLFQLDVAGKLGDVVQTLHQLYRASCLDKMGDQAAMITAILQSRLARTSFDKNRFQSISETLHMECKAEMVTPLVTNPGHVCVTDANLYFQPLNGYPKPVVQITLQNVRRIYKRRHGLMPLGLEVFCTENDLCSDIYLKFYNCQDRDELYFLIATYIENHVTEHTAESYMLQWQRGHISNYQYLLHLNNLADRSCNDLSQYPVFPWIIADYSSSVLDLTKPETFRDLSKPIGALNKERLDRLLTRYQEMPEPKFMYGSHYSSPGYVLFYLVRVAPEYMLCLQNGKFDHADRMFNSLAETWKNCLDGATDFKELIPEFYENDSSFLVNSLKLDLGKRQGGKMVEDVELPPWASGPDDFLQKSQEALESQYVSEHLHEWIDIIFGYKQKGSEAVEAHNVFHPLTYEGGVDLNSIMDPNEKVALLTQILEFGQTPKQLFTIPHPRRIVPKLKSLSRTSSHSVSMAESPVSPNEESFEDLTEESITLAWNNIAKLKLDERYKIHKEAITGIAVTCNGSSVFTTSQDSTLKMFSKESKTIQRSVSFSNMALSSCLILPGDTTVISSSWDNHIYFYSIAFGRQQDILMGHDDAVSKICWRDGRLYSASWDSTVKVWRCLPGETSSNKKHHSELLAELEHDVSVNTIDLNAANTMLASGTKEGTISVWDVPTATVLHQLPCHSGTVFDAAFSPDSRHLLSTGEDCCFKVIDVQTGMLISSVTAAEPQRCFKWDGNTILSGSQSGELMIWDLLGGKVMERIKGHTGAVTSMWMNEQCNSVITGGEDKQIMFWKLQY

Target ORF information:

RefSeq Version	XM_014952645.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax neutral sphingomyelinase activation associated factor (NSMAF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014952645.1

ORF Insert Sequence:

ATGCGCTTTC TTAACGAGCA GGCTATTCAG TTTTCGCTGC TGCTTCTTAA CCTGGAAGAA 
TATTACTTTG AACAGCACAC AGCTAATCAT ATTATAAATA AAGGTTGCAG AGATGAAAGA 
AAATTCAGAG GCTCTCTGAA AATCTGTTCA AAGTCAATTA TTTTTGAACC TGATGACAAT 
ATCCAACCCA TTATCAAGAT ACCACTGAGA GACTGCGTTA GTATAAAAGC CCCAGAAGAC 
AATGAAGCAA GTAACCCTTT CACACGGGAT GCATCTGGAG GGATTTCTGT TGTATGTAAT 
CAGGTTTTTC TCATTAAAGA AAGAAACATT ATTGCACCCT ATAAAACAGT AAGAGGTAGA 
ACAGAACACT TATTCCAACT GGATGTTGCT GGGAAACTAG GAGACGTGGT GCAAACTCTG 
CATCAGCTTT ACAGGGCCTC TTGTCTAGAT AAGATGGGAG ATCAAGCTGC CATGATTACT 
GCTATATTGC AATCGCGCTT GGCTAGGACG TCATTTGATA AAAACAGATT TCAAAGCATT 
TCTGAAACGC TGCATATGGA ATGTAAAGCA GAAATGGTGA CACCACTGGT GACTAATCCA 
GGGCATGTGT GTGTTACTGA TGCTAACTTG TACTTCCAGC CTCTTAATGG ATATCCTAAA 
CCAGTAGTAC AAATAACACT GCAAAACGTG CGTCGCATCT ATAAAAGACG ACATGGTTTA 
ATGCCACTGG GACTTGAAGT GTTTTGCACA GAAAATGATC TATGTTCTGA CATCTACTTG 
AAATTCTACA ACTGTCAAGA TCGAGATGAG CTCTATTTCC TTATTGCAAC ATATATAGAA 
AATCATGTAA CAGAGCATAC AGCTGAAAGT TATATGCTGC AATGGCAGCG AGGACATATA 
TCAAACTACC AGTATCTTCT TCATCTCAAC AATCTGGCTG ACAGGAGCTG CAACGATCTC 
TCCCAGTATC CTGTGTTTCC GTGGATCATA GCAGACTACT CTAGTTCAGT ATTAGATTTG 
ACAAAGCCTG AAACATTCCG TGACCTTAGT AAACCAATTG GTGCCCTGAA TAAGGAAAGG 
CTGGATAGAT TATTGACACG TTACCAGGAA ATGCCAGAGC CGAAATTCAT GTATGGGAGC 
CATTATTCCT CTCCGGGTTA TGTTTTGTTT TATCTCGTCA GAGTGGCTCC GGAATACATG 
CTGTGTCTGC AGAACGGAAA GTTTGATCAC GCTGACAGAA TGTTCAACAG TCTTGCAGAA 
ACCTGGAAAA ACTGTTTGGA TGGTGCAACA GATTTCAAAG AGTTGATTCC AGAATTTTAT 
GAAAATGATT CTAGTTTTCT AGTAAACAGT TTGAAGTTGG ACTTGGGAAA AAGGCAGGGT 
GGGAAGATGG TTGAAGATGT AGAGCTTCCC CCTTGGGCAT CAGGTCCTGA TGACTTTCTT 
CAGAAGAGCC AAGAGGCTTT AGAAAGTCAG TATGTATCGG AACATCTTCA TGAATGGATT 
GACATCATAT TTGGCTACAA GCAGAAAGGA AGTGAAGCAG TTGAAGCTCA CAATGTATTT 
CACCCATTAA CTTATGAAGG AGGAGTGGAT TTAAACAGTA TTATGGACCC CAATGAAAAA 
GTAGCTCTGC TGACACAAAT CTTGGAGTTT GGACAGACGC CAAAGCAATT GTTTACAATT 
CCTCATCCCC GAAGGATCGT ACCAAAGCTG AAAAGCCTGT CTCGAACGTC GAGCCACAGT 
GTTTCCATGG CTGAGTCTCC AGTTTCTCCA AATGAAGAAT CATTTGAAGA TTTGACAGAA 
GAAAGTATAA CACTTGCTTG GAACAATATT GCCAAACTAA AATTAGATGA GCGATATAAA 
ATTCACAAAG AGGCAATTAC TGGAATAGCA GTCACCTGCA ATGGGTCTTC TGTTTTCACT 
ACATCCCAGG ATTCAACTTT GAAGATGTTT TCCAAAGAAT CAAAGACAAT CCAGAGAAGT 
GTGTCTTTTT CAAACATGGC TTTGTCATCT TGTCTAATCT TACCAGGAGA TACCACTGTA 
ATAAGTTCTT CATGGGACAA TCATATATAT TTTTATTCAA TTGCATTTGG AAGACAGCAA 
GACATCTTAA TGGGGCATGA TGATGCAGTC AGTAAGATCT GTTGGCGTGA TGGGCGCTTA 
TATTCTGCCT CTTGGGATTC CACTGTCAAG GTGTGGCGCT GTCTGCCCGG GGAGACCTCC 
AGCAACAAAA AACACCACTC GGAGCTGCTC GCAGAGTTGG AACACGACGT TAGTGTAAAC 
ACCATCGACC TTAACGCTGC AAATACTATG TTGGCATCTG GAACCAAAGA GGGTACCATC 
AGTGTCTGGG ATGTTCCTAC AGCAACAGTC TTGCACCAGC TGCCGTGTCA TTCTGGGACT 
GTGTTTGATG CTGCTTTCAG TCCTGACAGC AGACACCTAC TCAGCACCGG AGAGGATTGT 
TGTTTCAAAG TAATAGATGT ACAAACCGGA ATGCTTATAT CTTCTGTAAC TGCTGCTGAG 
CCACAGAGAT GCTTCAAATG GGATGGAAAT ACCATCTTAT CGGGAAGTCA ATCTGGTGAA 
TTAATGATTT GGGACCTTCT TGGAGGAAAA GTTATGGAAA GAATCAAAGG ACATACAGGT 
GCTGTAACAT CCATGTGGAT GAACGAACAG TGCAACAGTG TTATTACAGG AGGGGAAGAC 
AAACAAATCA TGTTCTGGAA ACTGCAGTAC TAA