TLK2 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol TLK2

Entrez Gene ID 106896492

Full Name tousled like kinase 2

Gene Type protein-coding

Organism Calidris pugnax(ruff)

mRNA	Protein	Name
XM_014956551.1	XP_014812037.1	serine/threonine-protein kinase tousled-like 2 isoform X1
XM_014956553.1	XP_014812039.1	serine/threonine-protein kinase tousled-like 2 isoform X3
XM_014956554.1	XP_014812040.1	serine/threonine-protein kinase tousled-like 2 isoform X4
XM_014956552.1	XP_014812038.1	serine/threonine-protein kinase tousled-like 2 isoform X2

Pathways from BioSystems
Gene Ontology

The following TLK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TLK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa75706	XM_014956551.1 Latest version!	Calidris pugnax tousled like kinase 2 (TLK2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCa75707	XM_014956552.1 Latest version!	Calidris pugnax tousled like kinase 2 (TLK2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCa75708	XM_014956553.1 Latest version!	Calidris pugnax tousled like kinase 2 (TLK2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCa75709	XM_014956554.1 Latest version!	Calidris pugnax tousled like kinase 2 (TLK2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TLK2 ( XM_014956551.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014812037.1 Calidris pugnax tousled like kinase 2 (TLK2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa75706
Clone ID Related Accession (Same CDS sequence)	XM_014956551.1
Accession Version	XM_014956551.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	serine/threonine-protein kinase tousled-like 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091045.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG AATTGCATAG CCTGGACCCA CGACGGCAGG AACTACTGGA GGCCAGGTTC 
ACGGGAGTCG GGGTTGCTAA GGTTGGTGCT TCAAATTCCC TCCCTACCCC CCCAAGCCTC 
CGTCCGTCAT GCTCCGCACT GGCATTGGGT CCATTAAATA GTGAGTCTTC CAACCAGAGC 
TTGTGCAGCG TGGGTTCTCT GAGTGATAAA GAATTGGAGA CTCCCGAGAA AAAACAGAAT 
GACCAGCGGA ATAGAAAAAG GAAAGCAGAA CCCTATGAGA CCAGTCAAGG AAAAGGCACT 
CCTAGAGGAC ATAAAATTAG TGACTATTTT GAGTTTGCTG GGGGAAGTGG CCCAGGAACC 
AGTCCGGGTA GAAGTGTCCC GCCTGTCGCC AGATCGTCAC CGCAGCATTC CTTATCCAAC 
CCCTTGCCGC GGCGAGTGGA GCAGCCGCTG TATGGGGTAG ACGGCAGCAC AGCAAAGGAG 
CCGCAGGAGG AACACTCTGC TCTGCCAACG CTGATGTCGG TGATGCTGGC GAAGCAGCGG 
CTAGAGAATG AGCAGCTGGC ACAGAGGGGA GGTGGCCTCT GTTTTACTTT TGTCTCGGCC 
CAGCAAGGCA GCCCATCTTC TACCGGATCA GGGAACACTG AGCATTCCTG CACTTCCCAA 
AAACAGATTT CCATCCAGCA CAAACAGTCA CAGTCTGACC TCACAATGGA AAAAATATCT 
GCACTAGAAA ACAGTAAAAA CTCGGACTTG GAGAAGAAGG AGGGGAGGAT AGATGACTTA 
CTAAGAGCCA ACTGCGATTT GAGACGGCAG ATAGATGAAC AGCAAAAGAT GCTGGAGAAG 
TACAAGGAGC GGTTGAATAG ATGTGTAACA ATGAGCAAGA AGCTGCTTAT AGAAAAGTCA 
AAACAGGAGA AGATGGCATG CAGAGATAAG AGCATGCAGG ATCGATTGAG GCTAGGTCAC 
TTCACTACGG TGCGGCACGG GGCGTCCTTC ACCGAGCAGT GGACAGATGG CTACGCCTTC 
CAGAACCTCA TCAAGCAACA GGAAAGGATA AATTCCCAGC GGGAAGAAAT AGAAAGACAA 
CGAAAGATGT TGGCGAAACG GAAGCCACCT GCCATGGGAC AGACCCCTCC AGCAAGCAAC 
GAGCAGAAGC AGCGCAAGAA CAAGACCAAC GGAGCGGAAA ATGAAACGTT AACGTTAGCA 
GAATACCATG AACAGGAAGA AATTTTCAAA CTCCGACTAG GTCATCTTAA AAAGGAAGAA 
GCAGAGATCC AGGCGGAGTT GGAGCGGTTA GAGAGGGTTA GAAATCTACA TATCAGGGAA 
TTGAAAAGGA TACACAATGA AGATAATTCA CAATTTAAAG ACCATCCAAC GCTAAATGAT 
AGATATTTGT TGCTACATCT TCTGGGTAGA GGCGGCTTCA GTGAAGTATA TAAGGCATTC 
GATTTAACAG AACAGAGATA TGTAGCTGTG AAAATACACC AGTTAAATAA AAACTGGAGA 
GATGAGAAGA AAGAAAACTA TCACAAACAT GCGTGTAGAG AGTACAGAAT TCACAAAGAA 
CTGGATCATC CTCGAATAGT TAAGCTATAT GACTACTTTT CGCTGGATAC TGACTCGTTT 
TGTACAGTGT TAGAATACTG TGAGGGGAAT GATCTGGACT TCTACCTGAA ACAGCACAAG 
TTAATGTCGG AGAAAGAGGC ACGATCCATT ATCATGCAGA TTGTGAACGC TTTAAAATAC 
TTAAATGAAA TCAAACCTCC CATCATACAC TATGACCTTA AACCAGGTAA CATTCTTCTA 
GTCAATGGTA CTGCATGTGG AGAGATAAAA ATCACAGATT TTGGACTTTC CAAAATCATG 
GATGATGACA GCTACAACTC TGTTGATGGC ATGGAACTGA CATCGCAGGG GGCTGGTACT 
TACTGGTATT TGCCACCAGA ATGTTTTGTG GTTGGGAAAG AACCTCCAAA GATTTCAAAT 
AAAGTTGATG TCTGGTCAGT GGGAGTCATC TTCTACCAGT GTCTCTACGG CAGAAAGCCC 
TTTGGTCACA ACCAGTCACA GCAAGATATT CTGCAGGAGA ACACAATTCT GAAAGCTACC 
GAGGTGCAGT TCCCTCCAAA GCCAGTAGTC ACGCCAGAAG CAAAGGCATT TATCAGACGC 
TGTTTGGCCT ACCGAAAGGA GGATCGGATA GATGTCCAGC AGCTGGCCTG TGACCCCTAC 
TTGCTGCCTC ACATCCGCAA ATCTGTATCC ACAAGCAGCC CAGCTGGAGC TGCAATTGCA 
TCAACCTCTG GAACATCCAA TAACAGCTCT TCAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014812037.1
CDS	50..2368
Translation	MMEELHSLDPRRQELLEARFTGVGVAKVGASNSLPTPPSLRPSCSALALGPLNSESSNQSLCSVGSLSDKELETPEKKQNDQRNRKRKAEPYETSQGKGTPRGHKISDYFEFAGGSGPGTSPGRSVPPVARSSPQHSLSNPLPRRVEQPLYGVDGSTAKEPQEEHSALPTLMSVMLAKQRLENEQLAQRGGGLCFTFVSAQQGSPSSTGSGNTEHSCTSQKQISIQHKQSQSDLTMEKISALENSKNSDLEKKEGRIDDLLRANCDLRRQIDEQQKMLEKYKERLNRCVTMSKKLLIEKSKQEKMACRDKSMQDRLRLGHFTTVRHGASFTEQWTDGYAFQNLIKQQERINSQREEIERQRKMLAKRKPPAMGQTPPASNEQKQRKNKTNGAENETLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRIHNEDNSQFKDHPTLNDRYLLLHLLGRGGFSEVYKAFDLTEQRYVAVKIHQLNKNWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDSFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIIMQIVNALKYLNEIKPPIIHYDLKPGNILLVNGTACGEIKITDFGLSKIMDDDSYNSVDGMELTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFYQCLYGRKPFGHNQSQQDILQENTILKATEVQFPPKPVVTPEAKAFIRRCLAYRKEDRIDVQQLACDPYLLPHIRKSVSTSSPAGAAIASTSGTSNNSSSN

Target ORF information:

RefSeq Version	XM_014956551.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax tousled like kinase 2 (TLK2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014956551.1

ORF Insert Sequence:

ATGATGGAAG AATTGCATAG CCTGGACCCA CGACGGCAGG AACTACTGGA GGCCAGGTTC 
ACGGGAGTCG GGGTTGCTAA GGTTGGTGCT TCAAATTCCC TCCCTACCCC CCCAAGCCTC 
CGTCCGTCAT GCTCCGCACT GGCATTGGGT CCATTAAATA GTGAGTCTTC CAACCAGAGC 
TTGTGCAGCG TGGGTTCTCT GAGTGATAAA GAATTGGAGA CTCCCGAGAA AAAACAGAAT 
GACCAGCGGA ATAGAAAAAG GAAAGCAGAA CCCTATGAGA CCAGTCAAGG AAAAGGCACT 
CCTAGAGGAC ATAAAATTAG TGACTATTTT GAGTTTGCTG GGGGAAGTGG CCCAGGAACC 
AGTCCGGGTA GAAGTGTCCC GCCTGTCGCC AGATCGTCAC CGCAGCATTC CTTATCCAAC 
CCCTTGCCGC GGCGAGTGGA GCAGCCGCTG TATGGGGTAG ACGGCAGCAC AGCAAAGGAG 
CCGCAGGAGG AACACTCTGC TCTGCCAACG CTGATGTCGG TGATGCTGGC GAAGCAGCGG 
CTAGAGAATG AGCAGCTGGC ACAGAGGGGA GGTGGCCTCT GTTTTACTTT TGTCTCGGCC 
CAGCAAGGCA GCCCATCTTC TACCGGATCA GGGAACACTG AGCATTCCTG CACTTCCCAA 
AAACAGATTT CCATCCAGCA CAAACAGTCA CAGTCTGACC TCACAATGGA AAAAATATCT 
GCACTAGAAA ACAGTAAAAA CTCGGACTTG GAGAAGAAGG AGGGGAGGAT AGATGACTTA 
CTAAGAGCCA ACTGCGATTT GAGACGGCAG ATAGATGAAC AGCAAAAGAT GCTGGAGAAG 
TACAAGGAGC GGTTGAATAG ATGTGTAACA ATGAGCAAGA AGCTGCTTAT AGAAAAGTCA 
AAACAGGAGA AGATGGCATG CAGAGATAAG AGCATGCAGG ATCGATTGAG GCTAGGTCAC 
TTCACTACGG TGCGGCACGG GGCGTCCTTC ACCGAGCAGT GGACAGATGG CTACGCCTTC 
CAGAACCTCA TCAAGCAACA GGAAAGGATA AATTCCCAGC GGGAAGAAAT AGAAAGACAA 
CGAAAGATGT TGGCGAAACG GAAGCCACCT GCCATGGGAC AGACCCCTCC AGCAAGCAAC 
GAGCAGAAGC AGCGCAAGAA CAAGACCAAC GGAGCGGAAA ATGAAACGTT AACGTTAGCA 
GAATACCATG AACAGGAAGA AATTTTCAAA CTCCGACTAG GTCATCTTAA AAAGGAAGAA 
GCAGAGATCC AGGCGGAGTT GGAGCGGTTA GAGAGGGTTA GAAATCTACA TATCAGGGAA 
TTGAAAAGGA TACACAATGA AGATAATTCA CAATTTAAAG ACCATCCAAC GCTAAATGAT 
AGATATTTGT TGCTACATCT TCTGGGTAGA GGCGGCTTCA GTGAAGTATA TAAGGCATTC 
GATTTAACAG AACAGAGATA TGTAGCTGTG AAAATACACC AGTTAAATAA AAACTGGAGA 
GATGAGAAGA AAGAAAACTA TCACAAACAT GCGTGTAGAG AGTACAGAAT TCACAAAGAA 
CTGGATCATC CTCGAATAGT TAAGCTATAT GACTACTTTT CGCTGGATAC TGACTCGTTT 
TGTACAGTGT TAGAATACTG TGAGGGGAAT GATCTGGACT TCTACCTGAA ACAGCACAAG 
TTAATGTCGG AGAAAGAGGC ACGATCCATT ATCATGCAGA TTGTGAACGC TTTAAAATAC 
TTAAATGAAA TCAAACCTCC CATCATACAC TATGACCTTA AACCAGGTAA CATTCTTCTA 
GTCAATGGTA CTGCATGTGG AGAGATAAAA ATCACAGATT TTGGACTTTC CAAAATCATG 
GATGATGACA GCTACAACTC TGTTGATGGC ATGGAACTGA CATCGCAGGG GGCTGGTACT 
TACTGGTATT TGCCACCAGA ATGTTTTGTG GTTGGGAAAG AACCTCCAAA GATTTCAAAT 
AAAGTTGATG TCTGGTCAGT GGGAGTCATC TTCTACCAGT GTCTCTACGG CAGAAAGCCC 
TTTGGTCACA ACCAGTCACA GCAAGATATT CTGCAGGAGA ACACAATTCT GAAAGCTACC 
GAGGTGCAGT TCCCTCCAAA GCCAGTAGTC ACGCCAGAAG CAAAGGCATT TATCAGACGC 
TGTTTGGCCT ACCGAAAGGA GGATCGGATA GATGTCCAGC AGCTGGCCTG TGACCCCTAC 
TTGCTGCCTC ACATCCGCAA ATCTGTATCC ACAAGCAGCC CAGCTGGAGC TGCAATTGCA 
TCAACCTCTG GAACATCCAA TAACAGCTCT TCAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLK2 ( XM_014956552.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014812038.1 Calidris pugnax tousled like kinase 2 (TLK2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCa75707
Clone ID Related Accession (Same CDS sequence)	XM_014956552.1
Accession Version	XM_014956552.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	serine/threonine-protein kinase tousled-like 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091045.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG AATTGCATAG CCTGGACCCA CGACGGCAGG AACTACTGGA GGCCAGGTTC 
ACGGGAGTCG GGGTTGCTAA GGGTCCATTA AATAGTGAGT CTTCCAACCA GAGCTTGTGC 
AGCGTGGGTT CTCTGAGTGA TAAAGAATTG GAGACTCCCG AGAAAAAACA GAATGACCAG 
CGGAATAGAA AAAGGAAAGC AGAACCCTAT GAGACCAGTC AAGGAAAAGG CACTCCTAGA 
GGACATAAAA TTAGTGACTA TTTTGAGTTT GCTGGGGGAA GTGGCCCAGG AACCAGTCCG 
GGTAGAAGTG TCCCGCCTGT CGCCAGATCG TCACCGCAGC ATTCCTTATC CAACCCCTTG 
CCGCGGCGAG TGGAGCAGCC GCTGTATGGG GTAGACGGCA GCACAGCAAA GGAGCCGCAG 
GAGGAACACT CTGCTCTGCC AACGCTGATG TCGGTGATGC TGGCGAAGCA GCGGCTAGAG 
AATGAGCAGC TGGCACAGAG GGGAGGTGGC CTCTGTTTTA CTTTTGTCTC GGCCCAGCAA 
GGCAGCCCAT CTTCTACCGG ATCAGGGAAC ACTGAGCATT CCTGCACTTC CCAAAAACAG 
ATTTCCATCC AGCACAAACA GTCACAGTCT GACCTCACAA TGGAAAAAAT ATCTGCACTA 
GAAAACAGTA AAAACTCGGA CTTGGAGAAG AAGGAGGGGA GGATAGATGA CTTACTAAGA 
GCCAACTGCG ATTTGAGACG GCAGATAGAT GAACAGCAAA AGATGCTGGA GAAGTACAAG 
GAGCGGTTGA ATAGATGTGT AACAATGAGC AAGAAGCTGC TTATAGAAAA GTCAAAACAG 
GAGAAGATGG CATGCAGAGA TAAGAGCATG CAGGATCGAT TGAGGCTAGG TCACTTCACT 
ACGGTGCGGC ACGGGGCGTC CTTCACCGAG CAGTGGACAG ATGGCTACGC CTTCCAGAAC 
CTCATCAAGC AACAGGAAAG GATAAATTCC CAGCGGGAAG AAATAGAAAG ACAACGAAAG 
ATGTTGGCGA AACGGAAGCC ACCTGCCATG GGACAGACCC CTCCAGCAAG CAACGAGCAG 
AAGCAGCGCA AGAACAAGAC CAACGGAGCG GAAAATGAAA CGTTAACGTT AGCAGAATAC 
CATGAACAGG AAGAAATTTT CAAACTCCGA CTAGGTCATC TTAAAAAGGA AGAAGCAGAG 
ATCCAGGCGG AGTTGGAGCG GTTAGAGAGG GTTAGAAATC TACATATCAG GGAATTGAAA 
AGGATACACA ATGAAGATAA TTCACAATTT AAAGACCATC CAACGCTAAA TGATAGATAT 
TTGTTGCTAC ATCTTCTGGG TAGAGGCGGC TTCAGTGAAG TATATAAGGC ATTCGATTTA 
ACAGAACAGA GATATGTAGC TGTGAAAATA CACCAGTTAA ATAAAAACTG GAGAGATGAG 
AAGAAAGAAA ACTATCACAA ACATGCGTGT AGAGAGTACA GAATTCACAA AGAACTGGAT 
CATCCTCGAA TAGTTAAGCT ATATGACTAC TTTTCGCTGG ATACTGACTC GTTTTGTACA 
GTGTTAGAAT ACTGTGAGGG GAATGATCTG GACTTCTACC TGAAACAGCA CAAGTTAATG 
TCGGAGAAAG AGGCACGATC CATTATCATG CAGATTGTGA ACGCTTTAAA ATACTTAAAT 
GAAATCAAAC CTCCCATCAT ACACTATGAC CTTAAACCAG GTAACATTCT TCTAGTCAAT 
GGTACTGCAT GTGGAGAGAT AAAAATCACA GATTTTGGAC TTTCCAAAAT CATGGATGAT 
GACAGCTACA ACTCTGTTGA TGGCATGGAA CTGACATCGC AGGGGGCTGG TACTTACTGG 
TATTTGCCAC CAGAATGTTT TGTGGTTGGG AAAGAACCTC CAAAGATTTC AAATAAAGTT 
GATGTCTGGT CAGTGGGAGT CATCTTCTAC CAGTGTCTCT ACGGCAGAAA GCCCTTTGGT 
CACAACCAGT CACAGCAAGA TATTCTGCAG GAGAACACAA TTCTGAAAGC TACCGAGGTG 
CAGTTCCCTC CAAAGCCAGT AGTCACGCCA GAAGCAAAGG CATTTATCAG ACGCTGTTTG 
GCCTACCGAA AGGAGGATCG GATAGATGTC CAGCAGCTGG CCTGTGACCC CTACTTGCTG 
CCTCACATCC GCAAATCTGT ATCCACAAGC AGCCCAGCTG GAGCTGCAAT TGCATCAACC 
TCTGGAACAT CCAATAACAG CTCTTCAAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014812038.1
CDS	16..2268
Translation	MMEELHSLDPRRQELLEARFTGVGVAKGPLNSESSNQSLCSVGSLSDKELETPEKKQNDQRNRKRKAEPYETSQGKGTPRGHKISDYFEFAGGSGPGTSPGRSVPPVARSSPQHSLSNPLPRRVEQPLYGVDGSTAKEPQEEHSALPTLMSVMLAKQRLENEQLAQRGGGLCFTFVSAQQGSPSSTGSGNTEHSCTSQKQISIQHKQSQSDLTMEKISALENSKNSDLEKKEGRIDDLLRANCDLRRQIDEQQKMLEKYKERLNRCVTMSKKLLIEKSKQEKMACRDKSMQDRLRLGHFTTVRHGASFTEQWTDGYAFQNLIKQQERINSQREEIERQRKMLAKRKPPAMGQTPPASNEQKQRKNKTNGAENETLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRIHNEDNSQFKDHPTLNDRYLLLHLLGRGGFSEVYKAFDLTEQRYVAVKIHQLNKNWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDSFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIIMQIVNALKYLNEIKPPIIHYDLKPGNILLVNGTACGEIKITDFGLSKIMDDDSYNSVDGMELTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFYQCLYGRKPFGHNQSQQDILQENTILKATEVQFPPKPVVTPEAKAFIRRCLAYRKEDRIDVQQLACDPYLLPHIRKSVSTSSPAGAAIASTSGTSNNSSSN

Target ORF information:

RefSeq Version	XM_014956552.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax tousled like kinase 2 (TLK2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014956552.1

ORF Insert Sequence:

ATGATGGAAG AATTGCATAG CCTGGACCCA CGACGGCAGG AACTACTGGA GGCCAGGTTC 
ACGGGAGTCG GGGTTGCTAA GGGTCCATTA AATAGTGAGT CTTCCAACCA GAGCTTGTGC 
AGCGTGGGTT CTCTGAGTGA TAAAGAATTG GAGACTCCCG AGAAAAAACA GAATGACCAG 
CGGAATAGAA AAAGGAAAGC AGAACCCTAT GAGACCAGTC AAGGAAAAGG CACTCCTAGA 
GGACATAAAA TTAGTGACTA TTTTGAGTTT GCTGGGGGAA GTGGCCCAGG AACCAGTCCG 
GGTAGAAGTG TCCCGCCTGT CGCCAGATCG TCACCGCAGC ATTCCTTATC CAACCCCTTG 
CCGCGGCGAG TGGAGCAGCC GCTGTATGGG GTAGACGGCA GCACAGCAAA GGAGCCGCAG 
GAGGAACACT CTGCTCTGCC AACGCTGATG TCGGTGATGC TGGCGAAGCA GCGGCTAGAG 
AATGAGCAGC TGGCACAGAG GGGAGGTGGC CTCTGTTTTA CTTTTGTCTC GGCCCAGCAA 
GGCAGCCCAT CTTCTACCGG ATCAGGGAAC ACTGAGCATT CCTGCACTTC CCAAAAACAG 
ATTTCCATCC AGCACAAACA GTCACAGTCT GACCTCACAA TGGAAAAAAT ATCTGCACTA 
GAAAACAGTA AAAACTCGGA CTTGGAGAAG AAGGAGGGGA GGATAGATGA CTTACTAAGA 
GCCAACTGCG ATTTGAGACG GCAGATAGAT GAACAGCAAA AGATGCTGGA GAAGTACAAG 
GAGCGGTTGA ATAGATGTGT AACAATGAGC AAGAAGCTGC TTATAGAAAA GTCAAAACAG 
GAGAAGATGG CATGCAGAGA TAAGAGCATG CAGGATCGAT TGAGGCTAGG TCACTTCACT 
ACGGTGCGGC ACGGGGCGTC CTTCACCGAG CAGTGGACAG ATGGCTACGC CTTCCAGAAC 
CTCATCAAGC AACAGGAAAG GATAAATTCC CAGCGGGAAG AAATAGAAAG ACAACGAAAG 
ATGTTGGCGA AACGGAAGCC ACCTGCCATG GGACAGACCC CTCCAGCAAG CAACGAGCAG 
AAGCAGCGCA AGAACAAGAC CAACGGAGCG GAAAATGAAA CGTTAACGTT AGCAGAATAC 
CATGAACAGG AAGAAATTTT CAAACTCCGA CTAGGTCATC TTAAAAAGGA AGAAGCAGAG 
ATCCAGGCGG AGTTGGAGCG GTTAGAGAGG GTTAGAAATC TACATATCAG GGAATTGAAA 
AGGATACACA ATGAAGATAA TTCACAATTT AAAGACCATC CAACGCTAAA TGATAGATAT 
TTGTTGCTAC ATCTTCTGGG TAGAGGCGGC TTCAGTGAAG TATATAAGGC ATTCGATTTA 
ACAGAACAGA GATATGTAGC TGTGAAAATA CACCAGTTAA ATAAAAACTG GAGAGATGAG 
AAGAAAGAAA ACTATCACAA ACATGCGTGT AGAGAGTACA GAATTCACAA AGAACTGGAT 
CATCCTCGAA TAGTTAAGCT ATATGACTAC TTTTCGCTGG ATACTGACTC GTTTTGTACA 
GTGTTAGAAT ACTGTGAGGG GAATGATCTG GACTTCTACC TGAAACAGCA CAAGTTAATG 
TCGGAGAAAG AGGCACGATC CATTATCATG CAGATTGTGA ACGCTTTAAA ATACTTAAAT 
GAAATCAAAC CTCCCATCAT ACACTATGAC CTTAAACCAG GTAACATTCT TCTAGTCAAT 
GGTACTGCAT GTGGAGAGAT AAAAATCACA GATTTTGGAC TTTCCAAAAT CATGGATGAT 
GACAGCTACA ACTCTGTTGA TGGCATGGAA CTGACATCGC AGGGGGCTGG TACTTACTGG 
TATTTGCCAC CAGAATGTTT TGTGGTTGGG AAAGAACCTC CAAAGATTTC AAATAAAGTT 
GATGTCTGGT CAGTGGGAGT CATCTTCTAC CAGTGTCTCT ACGGCAGAAA GCCCTTTGGT 
CACAACCAGT CACAGCAAGA TATTCTGCAG GAGAACACAA TTCTGAAAGC TACCGAGGTG 
CAGTTCCCTC CAAAGCCAGT AGTCACGCCA GAAGCAAAGG CATTTATCAG ACGCTGTTTG 
GCCTACCGAA AGGAGGATCG GATAGATGTC CAGCAGCTGG CCTGTGACCC CTACTTGCTG 
CCTCACATCC GCAAATCTGT ATCCACAAGC AGCCCAGCTG GAGCTGCAAT TGCATCAACC 
TCTGGAACAT CCAATAACAG CTCTTCAAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLK2 ( XM_014956553.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014812039.1 Calidris pugnax tousled like kinase 2 (TLK2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCa75708
Clone ID Related Accession (Same CDS sequence)	XM_014956553.1
Accession Version	XM_014956553.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	serine/threonine-protein kinase tousled-like 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091045.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG AATTGCATAG CCTGGACCCA CGACGGCAGG AACTACTGGA GGCCAGGTTC 
ACGGGAGTCG GGGTTGCTAA GGTTGGTGCT TCAAATTCCC TCCCTACCCC CCCAAGCCTC 
CGTCCGTCAT GCTCCGCACT GGCATTGGGT CCATTAAATA GTGAGTCTTC CAACCAGAGC 
TTGTGCAGCG TGGGTTCTCT GAGTGATAAA GAATTGGAGA CTCCCGAGAA AAAACAGAAT 
GACCAGCGGA ATAGAAAAAG GAAAGCAGAA CCCTATGAGA CCAGTCAAGG AAAAGGCACT 
CCTAGAGGAC ATAAAATTAG TGACTATTTT GAGTTTGCTG GGGGAAGTGG CCCAGGAACC 
AGTCCGGGTA GAAGTGTCCC GCCTGTCGCC AGATCGTCAC CGCAGCATTC CTTATCCAAC 
CCCTTGCCGG CCCAGCAAGG CAGCCCATCT TCTACCGGAT CAGGGAACAC TGAGCATTCC 
TGCACTTCCC AAAAACAGAT TTCCATCCAG CACAAACAGT CACAGTCTGA CCTCACAATG 
GAAAAAATAT CTGCACTAGA AAACAGTAAA AACTCGGACT TGGAGAAGAA GGAGGGGAGG 
ATAGATGACT TACTAAGAGC CAACTGCGAT TTGAGACGGC AGATAGATGA ACAGCAAAAG 
ATGCTGGAGA AGTACAAGGA GCGGTTGAAT AGATGTGTAA CAATGAGCAA GAAGCTGCTT 
ATAGAAAAGT CAAAACAGGA GAAGATGGCA TGCAGAGATA AGAGCATGCA GGATCGATTG 
AGGCTAGGTC ACTTCACTAC GGTGCGGCAC GGGGCGTCCT TCACCGAGCA GTGGACAGAT 
GGCTACGCCT TCCAGAACCT CATCAAGCAA CAGGAAAGGA TAAATTCCCA GCGGGAAGAA 
ATAGAAAGAC AACGAAAGAT GTTGGCGAAA CGGAAGCCAC CTGCCATGGG ACAGACCCCT 
CCAGCAAGCA ACGAGCAGAA GCAGCGCAAG AACAAGACCA ACGGAGCGGA AAATGAAACG 
TTAACGTTAG CAGAATACCA TGAACAGGAA GAAATTTTCA AACTCCGACT AGGTCATCTT 
AAAAAGGAAG AAGCAGAGAT CCAGGCGGAG TTGGAGCGGT TAGAGAGGGT TAGAAATCTA 
CATATCAGGG AATTGAAAAG GATACACAAT GAAGATAATT CACAATTTAA AGACCATCCA 
ACGCTAAATG ATAGATATTT GTTGCTACAT CTTCTGGGTA GAGGCGGCTT CAGTGAAGTA 
TATAAGGCAT TCGATTTAAC AGAACAGAGA TATGTAGCTG TGAAAATACA CCAGTTAAAT 
AAAAACTGGA GAGATGAGAA GAAAGAAAAC TATCACAAAC ATGCGTGTAG AGAGTACAGA 
ATTCACAAAG AACTGGATCA TCCTCGAATA GTTAAGCTAT ATGACTACTT TTCGCTGGAT 
ACTGACTCGT TTTGTACAGT GTTAGAATAC TGTGAGGGGA ATGATCTGGA CTTCTACCTG 
AAACAGCACA AGTTAATGTC GGAGAAAGAG GCACGATCCA TTATCATGCA GATTGTGAAC 
GCTTTAAAAT ACTTAAATGA AATCAAACCT CCCATCATAC ACTATGACCT TAAACCAGGT 
AACATTCTTC TAGTCAATGG TACTGCATGT GGAGAGATAA AAATCACAGA TTTTGGACTT 
TCCAAAATCA TGGATGATGA CAGCTACAAC TCTGTTGATG GCATGGAACT GACATCGCAG 
GGGGCTGGTA CTTACTGGTA TTTGCCACCA GAATGTTTTG TGGTTGGGAA AGAACCTCCA 
AAGATTTCAA ATAAAGTTGA TGTCTGGTCA GTGGGAGTCA TCTTCTACCA GTGTCTCTAC 
GGCAGAAAGC CCTTTGGTCA CAACCAGTCA CAGCAAGATA TTCTGCAGGA GAACACAATT 
CTGAAAGCTA CCGAGGTGCA GTTCCCTCCA AAGCCAGTAG TCACGCCAGA AGCAAAGGCA 
TTTATCAGAC GCTGTTTGGC CTACCGAAAG GAGGATCGGA TAGATGTCCA GCAGCTGGCC 
TGTGACCCCT ACTTGCTGCC TCACATCCGC AAATCTGTAT CCACAAGCAG CCCAGCTGGA 
GCTGCAATTG CATCAACCTC TGGAACATCC AATAACAGCT CTTCAAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014812039.1
CDS	49..2199
Translation	MMEELHSLDPRRQELLEARFTGVGVAKVGASNSLPTPPSLRPSCSALALGPLNSESSNQSLCSVGSLSDKELETPEKKQNDQRNRKRKAEPYETSQGKGTPRGHKISDYFEFAGGSGPGTSPGRSVPPVARSSPQHSLSNPLPAQQGSPSSTGSGNTEHSCTSQKQISIQHKQSQSDLTMEKISALENSKNSDLEKKEGRIDDLLRANCDLRRQIDEQQKMLEKYKERLNRCVTMSKKLLIEKSKQEKMACRDKSMQDRLRLGHFTTVRHGASFTEQWTDGYAFQNLIKQQERINSQREEIERQRKMLAKRKPPAMGQTPPASNEQKQRKNKTNGAENETLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRIHNEDNSQFKDHPTLNDRYLLLHLLGRGGFSEVYKAFDLTEQRYVAVKIHQLNKNWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDSFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIIMQIVNALKYLNEIKPPIIHYDLKPGNILLVNGTACGEIKITDFGLSKIMDDDSYNSVDGMELTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFYQCLYGRKPFGHNQSQQDILQENTILKATEVQFPPKPVVTPEAKAFIRRCLAYRKEDRIDVQQLACDPYLLPHIRKSVSTSSPAGAAIASTSGTSNNSSSN

Target ORF information:

RefSeq Version	XM_014956553.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax tousled like kinase 2 (TLK2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014956553.1

ORF Insert Sequence:

ATGATGGAAG AATTGCATAG CCTGGACCCA CGACGGCAGG AACTACTGGA GGCCAGGTTC 
ACGGGAGTCG GGGTTGCTAA GGTTGGTGCT TCAAATTCCC TCCCTACCCC CCCAAGCCTC 
CGTCCGTCAT GCTCCGCACT GGCATTGGGT CCATTAAATA GTGAGTCTTC CAACCAGAGC 
TTGTGCAGCG TGGGTTCTCT GAGTGATAAA GAATTGGAGA CTCCCGAGAA AAAACAGAAT 
GACCAGCGGA ATAGAAAAAG GAAAGCAGAA CCCTATGAGA CCAGTCAAGG AAAAGGCACT 
CCTAGAGGAC ATAAAATTAG TGACTATTTT GAGTTTGCTG GGGGAAGTGG CCCAGGAACC 
AGTCCGGGTA GAAGTGTCCC GCCTGTCGCC AGATCGTCAC CGCAGCATTC CTTATCCAAC 
CCCTTGCCGG CCCAGCAAGG CAGCCCATCT TCTACCGGAT CAGGGAACAC TGAGCATTCC 
TGCACTTCCC AAAAACAGAT TTCCATCCAG CACAAACAGT CACAGTCTGA CCTCACAATG 
GAAAAAATAT CTGCACTAGA AAACAGTAAA AACTCGGACT TGGAGAAGAA GGAGGGGAGG 
ATAGATGACT TACTAAGAGC CAACTGCGAT TTGAGACGGC AGATAGATGA ACAGCAAAAG 
ATGCTGGAGA AGTACAAGGA GCGGTTGAAT AGATGTGTAA CAATGAGCAA GAAGCTGCTT 
ATAGAAAAGT CAAAACAGGA GAAGATGGCA TGCAGAGATA AGAGCATGCA GGATCGATTG 
AGGCTAGGTC ACTTCACTAC GGTGCGGCAC GGGGCGTCCT TCACCGAGCA GTGGACAGAT 
GGCTACGCCT TCCAGAACCT CATCAAGCAA CAGGAAAGGA TAAATTCCCA GCGGGAAGAA 
ATAGAAAGAC AACGAAAGAT GTTGGCGAAA CGGAAGCCAC CTGCCATGGG ACAGACCCCT 
CCAGCAAGCA ACGAGCAGAA GCAGCGCAAG AACAAGACCA ACGGAGCGGA AAATGAAACG 
TTAACGTTAG CAGAATACCA TGAACAGGAA GAAATTTTCA AACTCCGACT AGGTCATCTT 
AAAAAGGAAG AAGCAGAGAT CCAGGCGGAG TTGGAGCGGT TAGAGAGGGT TAGAAATCTA 
CATATCAGGG AATTGAAAAG GATACACAAT GAAGATAATT CACAATTTAA AGACCATCCA 
ACGCTAAATG ATAGATATTT GTTGCTACAT CTTCTGGGTA GAGGCGGCTT CAGTGAAGTA 
TATAAGGCAT TCGATTTAAC AGAACAGAGA TATGTAGCTG TGAAAATACA CCAGTTAAAT 
AAAAACTGGA GAGATGAGAA GAAAGAAAAC TATCACAAAC ATGCGTGTAG AGAGTACAGA 
ATTCACAAAG AACTGGATCA TCCTCGAATA GTTAAGCTAT ATGACTACTT TTCGCTGGAT 
ACTGACTCGT TTTGTACAGT GTTAGAATAC TGTGAGGGGA ATGATCTGGA CTTCTACCTG 
AAACAGCACA AGTTAATGTC GGAGAAAGAG GCACGATCCA TTATCATGCA GATTGTGAAC 
GCTTTAAAAT ACTTAAATGA AATCAAACCT CCCATCATAC ACTATGACCT TAAACCAGGT 
AACATTCTTC TAGTCAATGG TACTGCATGT GGAGAGATAA AAATCACAGA TTTTGGACTT 
TCCAAAATCA TGGATGATGA CAGCTACAAC TCTGTTGATG GCATGGAACT GACATCGCAG 
GGGGCTGGTA CTTACTGGTA TTTGCCACCA GAATGTTTTG TGGTTGGGAA AGAACCTCCA 
AAGATTTCAA ATAAAGTTGA TGTCTGGTCA GTGGGAGTCA TCTTCTACCA GTGTCTCTAC 
GGCAGAAAGC CCTTTGGTCA CAACCAGTCA CAGCAAGATA TTCTGCAGGA GAACACAATT 
CTGAAAGCTA CCGAGGTGCA GTTCCCTCCA AAGCCAGTAG TCACGCCAGA AGCAAAGGCA 
TTTATCAGAC GCTGTTTGGC CTACCGAAAG GAGGATCGGA TAGATGTCCA GCAGCTGGCC 
TGTGACCCCT ACTTGCTGCC TCACATCCGC AAATCTGTAT CCACAAGCAG CCCAGCTGGA 
GCTGCAATTG CATCAACCTC TGGAACATCC AATAACAGCT CTTCAAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLK2 ( XM_014956554.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014812040.1 Calidris pugnax tousled like kinase 2 (TLK2), transcript variant X4, mRNA.

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CloneID	OCa75709
Clone ID Related Accession (Same CDS sequence)	XM_014956554.1
Accession Version	XM_014956554.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	serine/threonine-protein kinase tousled-like 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091045.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG AATTGCATAG CCTGGACCCA CGACGGCAGG AACTACTGGA GGCCAGGTTC 
ACGGGAGTCG GGGTTGCTAA GGTTGGTGCT TCAAATTCCC TCCCTACCCC CCCAAGCCTC 
CGTCCGTCAT GCTCCGCACT GGCATTGGGT CCATTAAATA GTGAGTCTTC CAACCAGAGC 
TTGTGCAGCG TGGGTTCTCT GAGTGATAAA GAATTGGAGA CTCCCGAGAA AAAACAGAAT 
GACCAGCGGA ATAGAAAAAG GAAAGCAGAA CCCTATGAGA CCAGTCAAGG AAAAGGCACT 
CCTAGAGGAC ATAAAATTAG TGACTATTTT GAGTTTGCTG GGGGAAGTGG CCCAGGAACC 
AGTCCGGGTA GAAGTGTCCC GCCTGTCGCC AGATCGTCAC CGCAGCATTC CTTATCCAAC 
CCCTTGCCGT CTGACCTCAC AATGGAAAAA ATATCTGCAC TAGAAAACAG TAAAAACTCG 
GACTTGGAGA AGAAGGAGGG GAGGATAGAT GACTTACTAA GAGCCAACTG CGATTTGAGA 
CGGCAGATAG ATGAACAGCA AAAGATGCTG GAGAAGTACA AGGAGCGGTT GAATAGATGT 
GTAACAATGA GCAAGAAGCT GCTTATAGAA AAGTCAAAAC AGGAGAAGAT GGCATGCAGA 
GATAAGAGCA TGCAGGATCG ATTGAGGCTA GGTCACTTCA CTACGGTGCG GCACGGGGCG 
TCCTTCACCG AGCAGTGGAC AGATGGCTAC GCCTTCCAGA ACCTCATCAA GCAACAGGAA 
AGGATAAATT CCCAGCGGGA AGAAATAGAA AGACAACGAA AGATGTTGGC GAAACGGAAG 
CCACCTGCCA TGGGACAGAC CCCTCCAGCA AGCAACGAGC AGAAGCAGCG CAAGAACAAG 
ACCAACGGAG CGGAAAATGA AACGTTAACG TTAGCAGAAT ACCATGAACA GGAAGAAATT 
TTCAAACTCC GACTAGGTCA TCTTAAAAAG GAAGAAGCAG AGATCCAGGC GGAGTTGGAG 
CGGTTAGAGA GGGTTAGAAA TCTACATATC AGGGAATTGA AAAGGATACA CAATGAAGAT 
AATTCACAAT TTAAAGACCA TCCAACGCTA AATGATAGAT ATTTGTTGCT ACATCTTCTG 
GGTAGAGGCG GCTTCAGTGA AGTATATAAG GCATTCGATT TAACAGAACA GAGATATGTA 
GCTGTGAAAA TACACCAGTT AAATAAAAAC TGGAGAGATG AGAAGAAAGA AAACTATCAC 
AAACATGCGT GTAGAGAGTA CAGAATTCAC AAAGAACTGG ATCATCCTCG AATAGTTAAG 
CTATATGACT ACTTTTCGCT GGATACTGAC TCGTTTTGTA CAGTGTTAGA ATACTGTGAG 
GGGAATGATC TGGACTTCTA CCTGAAACAG CACAAGTTAA TGTCGGAGAA AGAGGCACGA 
TCCATTATCA TGCAGATTGT GAACGCTTTA AAATACTTAA ATGAAATCAA ACCTCCCATC 
ATACACTATG ACCTTAAACC AGGTAACATT CTTCTAGTCA ATGGTACTGC ATGTGGAGAG 
ATAAAAATCA CAGATTTTGG ACTTTCCAAA ATCATGGATG ATGACAGCTA CAACTCTGTT 
GATGGCATGG AACTGACATC GCAGGGGGCT GGTACTTACT GGTATTTGCC ACCAGAATGT 
TTTGTGGTTG GGAAAGAACC TCCAAAGATT TCAAATAAAG TTGATGTCTG GTCAGTGGGA 
GTCATCTTCT ACCAGTGTCT CTACGGCAGA AAGCCCTTTG GTCACAACCA GTCACAGCAA 
GATATTCTGC AGGAGAACAC AATTCTGAAA GCTACCGAGG TGCAGTTCCC TCCAAAGCCA 
GTAGTCACGC CAGAAGCAAA GGCATTTATC AGACGCTGTT TGGCCTACCG AAAGGAGGAT 
CGGATAGATG TCCAGCAGCT GGCCTGTGAC CCCTACTTGC TGCCTCACAT CCGCAAATCT 
GTATCCACAA GCAGCCCAGC TGGAGCTGCA ATTGCATCAA CCTCTGGAAC ATCCAATAAC 
AGCTCTTCAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014812040.1
CDS	49..2103
Translation	MMEELHSLDPRRQELLEARFTGVGVAKVGASNSLPTPPSLRPSCSALALGPLNSESSNQSLCSVGSLSDKELETPEKKQNDQRNRKRKAEPYETSQGKGTPRGHKISDYFEFAGGSGPGTSPGRSVPPVARSSPQHSLSNPLPSDLTMEKISALENSKNSDLEKKEGRIDDLLRANCDLRRQIDEQQKMLEKYKERLNRCVTMSKKLLIEKSKQEKMACRDKSMQDRLRLGHFTTVRHGASFTEQWTDGYAFQNLIKQQERINSQREEIERQRKMLAKRKPPAMGQTPPASNEQKQRKNKTNGAENETLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRIHNEDNSQFKDHPTLNDRYLLLHLLGRGGFSEVYKAFDLTEQRYVAVKIHQLNKNWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDSFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIIMQIVNALKYLNEIKPPIIHYDLKPGNILLVNGTACGEIKITDFGLSKIMDDDSYNSVDGMELTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFYQCLYGRKPFGHNQSQQDILQENTILKATEVQFPPKPVVTPEAKAFIRRCLAYRKEDRIDVQQLACDPYLLPHIRKSVSTSSPAGAAIASTSGTSNNSSSN

Target ORF information:

RefSeq Version	XM_014956554.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax tousled like kinase 2 (TLK2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014956554.1

ORF Insert Sequence:

ATGATGGAAG AATTGCATAG CCTGGACCCA CGACGGCAGG AACTACTGGA GGCCAGGTTC 
ACGGGAGTCG GGGTTGCTAA GGTTGGTGCT TCAAATTCCC TCCCTACCCC CCCAAGCCTC 
CGTCCGTCAT GCTCCGCACT GGCATTGGGT CCATTAAATA GTGAGTCTTC CAACCAGAGC 
TTGTGCAGCG TGGGTTCTCT GAGTGATAAA GAATTGGAGA CTCCCGAGAA AAAACAGAAT 
GACCAGCGGA ATAGAAAAAG GAAAGCAGAA CCCTATGAGA CCAGTCAAGG AAAAGGCACT 
CCTAGAGGAC ATAAAATTAG TGACTATTTT GAGTTTGCTG GGGGAAGTGG CCCAGGAACC 
AGTCCGGGTA GAAGTGTCCC GCCTGTCGCC AGATCGTCAC CGCAGCATTC CTTATCCAAC 
CCCTTGCCGT CTGACCTCAC AATGGAAAAA ATATCTGCAC TAGAAAACAG TAAAAACTCG 
GACTTGGAGA AGAAGGAGGG GAGGATAGAT GACTTACTAA GAGCCAACTG CGATTTGAGA 
CGGCAGATAG ATGAACAGCA AAAGATGCTG GAGAAGTACA AGGAGCGGTT GAATAGATGT 
GTAACAATGA GCAAGAAGCT GCTTATAGAA AAGTCAAAAC AGGAGAAGAT GGCATGCAGA 
GATAAGAGCA TGCAGGATCG ATTGAGGCTA GGTCACTTCA CTACGGTGCG GCACGGGGCG 
TCCTTCACCG AGCAGTGGAC AGATGGCTAC GCCTTCCAGA ACCTCATCAA GCAACAGGAA 
AGGATAAATT CCCAGCGGGA AGAAATAGAA AGACAACGAA AGATGTTGGC GAAACGGAAG 
CCACCTGCCA TGGGACAGAC CCCTCCAGCA AGCAACGAGC AGAAGCAGCG CAAGAACAAG 
ACCAACGGAG CGGAAAATGA AACGTTAACG TTAGCAGAAT ACCATGAACA GGAAGAAATT 
TTCAAACTCC GACTAGGTCA TCTTAAAAAG GAAGAAGCAG AGATCCAGGC GGAGTTGGAG 
CGGTTAGAGA GGGTTAGAAA TCTACATATC AGGGAATTGA AAAGGATACA CAATGAAGAT 
AATTCACAAT TTAAAGACCA TCCAACGCTA AATGATAGAT ATTTGTTGCT ACATCTTCTG 
GGTAGAGGCG GCTTCAGTGA AGTATATAAG GCATTCGATT TAACAGAACA GAGATATGTA 
GCTGTGAAAA TACACCAGTT AAATAAAAAC TGGAGAGATG AGAAGAAAGA AAACTATCAC 
AAACATGCGT GTAGAGAGTA CAGAATTCAC AAAGAACTGG ATCATCCTCG AATAGTTAAG 
CTATATGACT ACTTTTCGCT GGATACTGAC TCGTTTTGTA CAGTGTTAGA ATACTGTGAG 
GGGAATGATC TGGACTTCTA CCTGAAACAG CACAAGTTAA TGTCGGAGAA AGAGGCACGA 
TCCATTATCA TGCAGATTGT GAACGCTTTA AAATACTTAA ATGAAATCAA ACCTCCCATC 
ATACACTATG ACCTTAAACC AGGTAACATT CTTCTAGTCA ATGGTACTGC ATGTGGAGAG 
ATAAAAATCA CAGATTTTGG ACTTTCCAAA ATCATGGATG ATGACAGCTA CAACTCTGTT 
GATGGCATGG AACTGACATC GCAGGGGGCT GGTACTTACT GGTATTTGCC ACCAGAATGT 
TTTGTGGTTG GGAAAGAACC TCCAAAGATT TCAAATAAAG TTGATGTCTG GTCAGTGGGA 
GTCATCTTCT ACCAGTGTCT CTACGGCAGA AAGCCCTTTG GTCACAACCA GTCACAGCAA 
GATATTCTGC AGGAGAACAC AATTCTGAAA GCTACCGAGG TGCAGTTCCC TCCAAAGCCA 
GTAGTCACGC CAGAAGCAAA GGCATTTATC AGACGCTGTT TGGCCTACCG AAAGGAGGAT 
CGGATAGATG TCCAGCAGCT GGCCTGTGAC CCCTACTTGC TGCCTCACAT CCGCAAATCT 
GTATCCACAA GCAGCCCAGC TGGAGCTGCA ATTGCATCAA CCTCTGGAAC ATCCAATAAC 
AGCTCTTCAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TLK2 cDNA ORF clone, Calidris pugnax(ruff)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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