LOC106899270 cDNA ORF clone, Calidris pugnax(ruff)

  • Gene

  • Clones

  • gRNAs

The following LOC106899270 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC106899270 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa78934 XM_014961016.1
Latest version!
Calidris pugnax collagen alpha-1(VII) chain-like (LOC106899270), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCa78934 XM_014961015.1
Latest version!
Calidris pugnax collagen alpha-1(VII) chain-like (LOC106899270), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCa78934 XM_014961017.1
Latest version!
Calidris pugnax collagen alpha-1(VII) chain-like (LOC106899270), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCa78935 XM_014961018.1
Latest version!
Calidris pugnax collagen alpha-1(VII) chain-like (LOC106899270), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCa78936 XM_014961019.1
Latest version!
Calidris pugnax collagen alpha-1(VII) chain-like (LOC106899270), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCa78934
    Clone ID Related Accession (Same CDS sequence) XM_014961016.1 , XM_014961017.1 , XM_014961015.1
    Accession Version XM_014961016.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7515bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product collagen alpha-1(VII) chain-like isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTTTGTG CCCAGATCCT TGCCCTGGCA GTGGCTGTGG TGCAGCACAG AGCTGTGGCC 
    GTTCATGAAG CATGCAGGAC GGCGGAGGTG GCCGACATTC TGTTCCTCGT GGGACAGTCA
    CAGGGCACAG GAAGGGAGAG CTCCTGGCTG GTCAAGGACT TCATCAGCAG CATGGCTCGT
    TCCTTTGAGA ACGTGGTGAT GGGCAAAGGA GGCATCCGGC TGGCAGTGGC ACTCTACGGG
    GAGAAACCAA GGATGAGTAT TGAGCTTACG GATTACGTGA CCATCGAAGA AATGTTGGTG
    GCAATTCAGG ATCTCTCCTT CAAAGGTGGA AGCTTAAAAA CAGGGTCAGC CTTGGCCTTT
    GCAGCTCGTG CCATGTCTCG TCTGGACACA CTGCGAGAGG ATGCAGCAAA GGTGGTTGTT
    TTGATCACGG ATGGAAAATC CAGCAACTCA GTTGAAGATG AAGCCCGGGT CCTGCAGGAT
    TTGGGGGTGA CGGTGTTTGC CGTAGGGATT AAGGGTGCTG ACAAGAATGA ATTGAACACA
    ATAGCTTCAG AGCCCACTGC AGAGCATGTG CTTTATGTTG AAGATTTCCA CCTCCTCCAT
    AACGTGGCCC CCAAACTCTC TCGAAGGCTC TGTTTCACTG CCTCGGAGCC ACCACGACCC
    ATCAAACAGA CTGTGCAAGC TGAGAAGATC GTCGGCCCCC GGGATTTGTA TGTCAGTGAG
    CACAGCCACA GCACGCTGAG ACTCACCTGG ATGCCAGCCA CGGGGAAGGT GACGGGCTAC
    CATGTCCATC TGCATCCCTT GCTGCCTTCA GGGCAGCCCG TTTCAGAAGA CCAGCAGCAG
    ATTGTGGTGG ACGGTGACAA AAGTACCGTG CTGGTGACTG ATCTGAAACC CAACACCAAG
    TATGTCTTCA CGGTGAGGGC CATCTATGCA GATACTGTTG GGGAGTCAGC GGTTGTCAAG
    GGGAAAACAA CACCTGTGCC TCCAGTCACT AATTTCCGTG TGATAGAAGA AGGACTTTTT
    AGCTTGAAGG TGGCTTGGAC ACCTCCGCTT GGCAAGCTGG AGGGCTACAA GATCTACATC
    CCCAGAGCCA ACAGACCAGG AATGACCTAC GAGCAGGTTC TGCGGGGTGA TGTCTCATCC
    CACGTGCTGG AGAACTTGCA GGAGGACAGA GAATACAGCA TCAGCATCTA CGCCGTGTAC
    CCCCAGGGCC CCAGCCAGCC TGTGGCTGCT GTGGGGAGAA CATTGAAGCT CCTGCCCGTG
    AAAAGCATCT TGCTGCAGAA TGAGACCACC AACACGCTCC AGGCAAGGTG GACACCCATC
    CGGGGAGCAA CGGGGTACAG GCTCACCTGG ACATCAGCAG AGGGCAGCAT CCAAGATGTG
    AACCTCAGCA ACACCTACAG CTACTACATG ATCCAGGGGC TGCAGCCTGG CACGGAGTAC
    ACTGTTACCA TCAACCCCAT CTTTGGGGAC GTCGAGGGAC CAGTGGTGAG CAGCAAAGCA
    ACGACGGTGG CATCGAGCAC CGTCCAGATG CTGAAAGTGA CTGAGATATC TACCAACAGT
    GCTCTGGTCT CCTGGGACTC AGTTGCTGGG GCCACTGGGT ATCGAGTGGC TTGGGGTCCT
    ACACCAGAGT TCTTTGGCAA AGACCGTCCT CGCCAGCTGG CCCTCAACAG GTCAATCACA
    GCCTACCAGC TCCGCAACCT GGCCCATGAC ACCGAGTACG TGATTTCCCT CTACGTGCTC
    TTTGGCTCCG TGGAAGGACC AGGGATCACA ACCTCAGCCA GGACATCTCC TCTTGGCTAC
    GTCTCCAATT TCAAGGTGAT GTCCTACACC AGCACAAGCC TGTCTCTGGT GTGGAGTGCC
    ACAGCAGCTG CCACCAAATT CAAAGTCACC TGGAGCCCTG TGGGAGCAGA AAAAGAAAAA
    CAGGTTGCCA AGACTCAGTA CCTGGGCAGT AGGGTGCTGG CTTACCAGAT TGACCAGTTA
    CTGCCCAACA CCCTGTATAA AGTCAGCGTT CGGGCTGTCT TCAACAAGAC TGAGGGACCA
    GAGGTGACTC TGACTCACCG CACAGCGCCT CTTGCAGACT CCAACCCCAT CCAGGCCGTT
    GAAGATCTGA GGATTACTGA CACCGGCGTA AATAGCTTGA AGCTGTCTTG GAGGAGACTT
    CCTGGGGTAA CTCATTATAA GATATCCTGG GCGCCGTTCA ATGGTGGCTT GGAAACCTCC
    CAGGTGGTTG CAGCAGAGAC GGTCTCCTTC ACGATCTCCA ACCTGAAGGA GAGCACCACC
    TACACCATCT GCATGTCTGC AATGATCGGG GGACAGGAGG GAAGCCCCAC ACTCCTCACA
    GCCAAAACTT TGGATTTACC CAAAGTGACT GAGTTCATGG TGCAGGAGGC TGCGGAGACC
    AGCGTTTTGC TGACATGGAC AGCTGTTCCT GGGGCATCCA CTTATCTATT AACATGGCGC
    CTGTCCTCAG CTTCTGATCT TTCCCAGGAA CTGCTGTCAG CAGCTTCTAG ATCGTATCGA
    GTGACAGGAC TGAGCACCAA AGAAATGTAT GTGTTTTCCA TAAGACCAGT CTTTGGAGAT
    ACGGAGGGAC CAGAGACAAC ACTCCTTGGG CAAACAGCGG GACCTCACAG AGAGTCACCC
    ACACCCATCA CTCCAGTGAC TTCTACAAAA CTGGGTACGA TGAGATTCCC ATCCTTATCT
    CCTAGCAAGG TGGGGACCAC TCCTCTTCCA CTGGCCACCA CCACAACAGT ACCACCAACT
    CCAGCAACCC TACTGTCGAC CACTGCAGTG TCCACACTGC TGACAACTCT CCCTGGGCCA
    ATCTGTGGCA AGTTCAAAGC AGACATTGGG TTCCTGGTGG ACGAGTCCTC GAGTATTGGC
    CAGAGCAATT TCAACAAAGT GAAGGATTTC CTCTTCCGGA TCATTTCCTA TTTCCCGAAG
    ATTGGGCCTG AAGGGACACA GGTTGCTGTT GCTCAGTACA GCGAGGAGCC CAGGGCAGCC
    TTCTACTTCA ACCAGCACCG GGACCGCAAC AGTGCCCTGA AAGCCGTCAA GGGACTGAGC
    TACGCTGGGG GCAACACAAA AACAGGTCGT GGCATAACCT ACATGCTCAA GGAGTTATTT
    CAGTCCTCCA GAGGGATGAG GCCAGATTTC CCTCACGTGC TGGTGCTGGT GACCGATGGG
    CGATCCCAGG ATGATGTGTT GCTACCAGCC AGAGCAGCCC ATGAGTTGGG GATCCGCGTC
    ATTGCTGTGG GTGTCTCAGG AGCTGACCCC GTTGAGCTCA ACAGCATCTT GCTGCAGCAG
    AACCTCCAGA ATGTTTTCTA CGTCAGCACG TTTGATGACT TCCCCCAGAT CCTCCGAGAG
    CTGATAGAGG TCATCTGTTC TGACCCCGAG CCTTCAGGGC CTCAACTACC ATCTGGGGAG
    AGCTTCAGGA GTTCACCAGC TCCTCATGGA GGCTATGACC CATACAGCTT TACTACAAAG
    GGAGAGAAAG GAGAGAGGGG TCTACCTGGG AAGGATGGAA TTCCCGGGCT GCCGGGCAGA
    CCGGGGCGGA CAGGTCCTCC TGGTCCCCCT GGACTCATGG GTCTCCCTGG CATCCAAGGT
    GATACTGGCT CTCCTGGCTA TCCTGGACCA GCGGGGCCAA AAGGAGAGAG AGGCGAACCA
    GGCTATGTCC TGGGAGGAGT AGAGGTGATT CCTGGCCGAA ATGGGCAGCC TGGCCCCCCT
    GGACAGAAAG GGCAGCCGGG GGTTCCTGGG GTAGCAGGAC CACCAGGTTT ACCAGGGCCA
    CAGGGGCCAC CAGGCATCTC CATCAAGGGT GAACGTGGGG ATTCAGGTAC CAGGGGCCCT
    CGTGGGAGGA ATGGCCTCAA GGGTGACAGA GGAGGAATGG GAGAGCCGGG GAAACCTGGG
    TTGCCAGGCC CAGTAGGGCT AGATGGTGTT CCTGGATTGC CTGGACCAAG GGGAGAAAAG
    GGTGTGATGG GACCTCCAGG AGCACCAGGA CCAAAGGGTA ACAAAGGGCA AGGTGGTGTG
    AAAGGGGAAA AGGGACTCCC AGGACCTCCA GGGCAGAAGG GAGACCAGGG GAACCCAGGA
    TTTCCAGGTG CTCCAGCTAT GGGGGTTTTG GGACCTCCAG GCAAGAAAGG TGTCAGGGGT
    GATATTGGAC CAACAGGGGC CCTGGGACCA CAAGGAGACA AAGGAATACA AGGAGACAAA
    GGAGAGAAGG GAAGCCCAGG TTTCGGGATT CCTGGTCAAC CTGGGCTGAA GGGAGATCCA
    GGCGACAGGG GAAATGTAGG TTTGTCGGGC AAACCAGGTC AGAAGGGGGA TAGAGGTCTG
    AAGGGAGAGA AAGGTGAACC AGGACTACCT GGGAAACCAG GCGAGACCGG ACTAAGAGGT
    AAAGATGGAG AACCAGGGGA GAAAGGCACC TCTGGGACCA AGGGAGAAGC TGGTGTCCCT
    GGAGAACCAG GAGAGAGAGG AATCAGGGGG CCACCTGGAC TCCCAGGACG GCCTGGAGAG
    CAAGGGATAA AAGGCGATAC AGGACCACCT GGAAAGGATG GAATCACTGG AGAGAAGGGA
    GACAAGGGCG AAGCTGGTGA GCCTGGGCTG CCTGGGACTC CTGGAAGCCT TGTGTCTTCA
    CTGGAGAGCA CCATCACCCT GAAAGGCGAC AAGGGGGATC GTGGGAAGGA TGGCTTGAAA
    GGCGAAAGAG GTCCTCCAGG CCCAAAGGGA GAACCCGGTC CACCAGGCAA AGGAGTGGAA
    ATGAAGGATC TGGAGAGGCT TTTTGAAGCA AATGGTATCA AGCTGGCACT GCTGAAGGAC
    CTCTCCAATG CACTCCTGCA GGATGGGCTG GATGGGCTGG TGTGGCAGCT GGGTGGCTCC
    AGGACAAGCA AGACCTCCAG GAGAAAGCAA GCAGTTCTTC ATGCTACTGA GCATGGCGAT
    TCACTGGTGT TTGATACTTC TCCTGGTGCC CTGGCCAGCA CTGAGGTGAC AGAAGAAGCA
    CAGAGCCTGG AGGTGGAGGC TCAGTTTAGG GTGCCGGTGT CTGGAGGACT CCTTAAGGGT
    CCATCTGCTG GTGGTTCTGA ACCTGAGTAC CAGCCCCTAG GAAACCCTCC TGAGTCCTTG
    GAGAGAACAT CTGAGGAAGG AAAGGAGGAG AGAGACCCAA GATGGACAAT CACTTCTCTC
    AGTGGTAGCT TGGCTTTGCC CCCACTGCAC CCAGAAGATG ATCTGCAAGG AAACCAGTCC
    ATGGAGCTTC TTGAGTCCCT GGAGGGGAGA GTGGAAGGAC AACCACAGCA GGACACTAGC
    CACGAAAATG AGAATTTAGA CGACGGCTCT GCGCTCCCAA GGCACGTTAG AGCCCAGAGA
    TCTGCTGAGG ACCAGAGACA CGTGGTAAAG ACCCCATTTC ACCACGACAG CTTCGGGCTG
    GCCTTTGTGC AAGAGCAGAG CCATCCGTGT GACCCAGGAG GAAACGGCGA CCTTTCCTTG
    TTGGCTCCAT CACAGGTTCA TCCCCCTGGT GCTCCAGGAG CTCGGAAATC CCCATCTGGA
    TCCACGGCAT ACCCAGGCCC TCATCACCCA AGGAGAATTA AGAAGGAGGA AGCTGGTCCA
    CCAGATCCAC CATCCCATGG CCAAAAGGGA GAAATGGGAG CTCCGGGAGT CCGTGGAGAC
    AAAGGAGAAA AGGGGCAGCC TGGGGAGATG GGGGAACCTG GAGAAAAGGG CATCAAAGGA
    GACAGAGGTG AAAATGGTCA AAAGGGAGAA CCAGGAATTG GGTTCAGAGG CCCTGTTGGT
    CAGGCTGGAC CACCTGGATT TAAAGGTGAG CCAGGGGCTC CAGGGCCTCC AGGAGCTCAG
    GGAGTCCAGG GAATTCGTGG CAATGCCGGC ACCCCCGGGT CACAAGGGGA AAGAGGGGCT
    CCAGGTCTGC CTGGGATGCC AGGACAAAAG GGAGAGCGTG GGAAGAGAGG ACGGAATGGA
    TTTGCTGGGC CCTCAGGACC ATCAGGATCC CCAGGAAAAG AGGGGATTTC TGGGACACCT
    GGACCCAAAG GCAACAAGGG AGAAATCGGC GTTGGAGCAG CTGGCCCAAG AGGTCCTCGT
    GGACTGCCTG GCCCTCGGGG TGACGAGGGC ATCGTGGGAG TCCGTGGCCC CACCGGCATG
    ACGGGTCTAA AAGGCTTCCC GGGTGTGAAG GGCGAAAGAG GTGATCGGGG ACTTCTGGGA
    CCCAAAGGAG AAAGAGGTGA GCCCATGACT ATTTTTGGAC CCCAAGGCTA TAAAGGCAGT
    AAAGGAGATC CTGGTGAACG GGGCCTGCCA GGTTTTGACG GAGACAAAGG CGAGAAAGGA
    GAGGATGGGC CTATAGGAGA AAAGGGAGTC AAAGGTGAAG CTGGCTCCAA GGGAGTGATG
    GGGCTTTTTG GAACAAGAGG ACCAGTGGGA CAGAAGGGGG AGCCAGGAGA ACCAGGCTTG
    CCGGGCTCTG CTGGTGCAGC AGGTCTTAAT GGGAAGAACG GAAACAAGGG AGCCAAAGGT
    GACAGAGGTC TTCAGGGGCA GAAGGGGAAG CCGGGAGGAA AAGGTGATCC TGGGGCCACT
    GGTGACATTG GACAGAAAGG AACAAAGGGC TTACGAGGGC TTCCAGGACG CATCGGTGCT
    CCTGGAGCTG AAGGCATGAA GGGAGAAATT GGCAGTCCTG GAAAACCAGG AATCCCAGGA
    TCAGATGGAC CACACGGACA AAAGGGTGAA AAGGGAACAT CAGGAGACGA TGGTGCCTCG
    GGGATTCCTG GGGAGAAAGG AGAAAAGGGA GCCAAAGGAG TTCCTGGCTT GGGAGGCTTC
    AAAGGACAAA TGGGATGGCC TGGGAGGACT GGAGTTGCTG GACCAGATGG CCCTCAAGGG
    CCACAGGGTG AACCAGGGCC CCAGGGTCCA CGAGGGAAAA GAGGGAGACC TCAGCTCTGC
    CTCAGGGGAC CCCCAGGCAT GGATGGGAGA AAAGGAGAAA TGGGTGAAAA TGGTCCAGCC
    GGCCAGAAAG GAGAGAAGGG AGATCCTGGT CTTTCTGAGG AAGAGGTGAA GGAGGTGGTC
    AGGAGCGAAA TGAGAGGCAG ATGCGATTGT GGAGGCGAGT TGGGAAGGAC ACTCATGGAA
    AGGGGCCTGG AGGCAACTGA AATCAACAGG CAGCTCCAAG GGTTCCAAAA GTTCCAGCTT
    TGGGAACGCA CCAGAAGGGA TGGTGAAAGC CAGGAAGAAA TGCTGAACAA AACCCAGGGG
    ATGACCATGT TGATGACAAT GGTGAAGCCC GTCATGGAGC CACCTTCACT TATTGCAGCG
    GCTCCCCGGG CGCAGCAGAT AACAGGTCCC TCTCAGCGCA AGCGGCGAGA AGCCCACAGT
    CAATTACCAG CCCCCTGCCT GCAGCCGATG GATGAAGGCT CCTGCCAGCA CTATGCCCTG
    CTCTGGTATT ACCACCTGGA GGCAAACACC TGCCGGCCCT TCATCTTTGG GGGATGCCGA
    GGGAACAGCA ACCGCTTCGA AACCAAGTGG AAGTGTGAGC AGAAGTGCAA AACCTCAGCA
    GCCAGAGGGA GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014816502.1
    CDS249..7763
    Translation

    Target ORF information:

    RefSeq Version XM_014961016.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax collagen alpha-1(VII) chain-like (LOC106899270), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014961016.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTTTGTG CCCAGATCCT TGCCCTGGCA GTGGCTGTGG TGCAGCACAG AGCTGTGGCC 
    GTTCATGAAG CATGCAGGAC GGCGGAGGTG GCCGACATTC TGTTCCTCGT GGGACAGTCA
    CAGGGCACAG GAAGGGAGAG CTCCTGGCTG GTCAAGGACT TCATCAGCAG CATGGCTCGT
    TCCTTTGAGA ACGTGGTGAT GGGCAAAGGA GGCATCCGGC TGGCAGTGGC ACTCTACGGG
    GAGAAACCAA GGATGAGTAT TGAGCTTACG GATTACGTGA CCATCGAAGA AATGTTGGTG
    GCAATTCAGG ATCTCTCCTT CAAAGGTGGA AGCTTAAAAA CAGGGTCAGC CTTGGCCTTT
    GCAGCTCGTG CCATGTCTCG TCTGGACACA CTGCGAGAGG ATGCAGCAAA GGTGGTTGTT
    TTGATCACGG ATGGAAAATC CAGCAACTCA GTTGAAGATG AAGCCCGGGT CCTGCAGGAT
    TTGGGGGTGA CGGTGTTTGC CGTAGGGATT AAGGGTGCTG ACAAGAATGA ATTGAACACA
    ATAGCTTCAG AGCCCACTGC AGAGCATGTG CTTTATGTTG AAGATTTCCA CCTCCTCCAT
    AACGTGGCCC CCAAACTCTC TCGAAGGCTC TGTTTCACTG CCTCGGAGCC ACCACGACCC
    ATCAAACAGA CTGTGCAAGC TGAGAAGATC GTCGGCCCCC GGGATTTGTA TGTCAGTGAG
    CACAGCCACA GCACGCTGAG ACTCACCTGG ATGCCAGCCA CGGGGAAGGT GACGGGCTAC
    CATGTCCATC TGCATCCCTT GCTGCCTTCA GGGCAGCCCG TTTCAGAAGA CCAGCAGCAG
    ATTGTGGTGG ACGGTGACAA AAGTACCGTG CTGGTGACTG ATCTGAAACC CAACACCAAG
    TATGTCTTCA CGGTGAGGGC CATCTATGCA GATACTGTTG GGGAGTCAGC GGTTGTCAAG
    GGGAAAACAA CACCTGTGCC TCCAGTCACT AATTTCCGTG TGATAGAAGA AGGACTTTTT
    AGCTTGAAGG TGGCTTGGAC ACCTCCGCTT GGCAAGCTGG AGGGCTACAA GATCTACATC
    CCCAGAGCCA ACAGACCAGG AATGACCTAC GAGCAGGTTC TGCGGGGTGA TGTCTCATCC
    CACGTGCTGG AGAACTTGCA GGAGGACAGA GAATACAGCA TCAGCATCTA CGCCGTGTAC
    CCCCAGGGCC CCAGCCAGCC TGTGGCTGCT GTGGGGAGAA CATTGAAGCT CCTGCCCGTG
    AAAAGCATCT TGCTGCAGAA TGAGACCACC AACACGCTCC AGGCAAGGTG GACACCCATC
    CGGGGAGCAA CGGGGTACAG GCTCACCTGG ACATCAGCAG AGGGCAGCAT CCAAGATGTG
    AACCTCAGCA ACACCTACAG CTACTACATG ATCCAGGGGC TGCAGCCTGG CACGGAGTAC
    ACTGTTACCA TCAACCCCAT CTTTGGGGAC GTCGAGGGAC CAGTGGTGAG CAGCAAAGCA
    ACGACGGTGG CATCGAGCAC CGTCCAGATG CTGAAAGTGA CTGAGATATC TACCAACAGT
    GCTCTGGTCT CCTGGGACTC AGTTGCTGGG GCCACTGGGT ATCGAGTGGC TTGGGGTCCT
    ACACCAGAGT TCTTTGGCAA AGACCGTCCT CGCCAGCTGG CCCTCAACAG GTCAATCACA
    GCCTACCAGC TCCGCAACCT GGCCCATGAC ACCGAGTACG TGATTTCCCT CTACGTGCTC
    TTTGGCTCCG TGGAAGGACC AGGGATCACA ACCTCAGCCA GGACATCTCC TCTTGGCTAC
    GTCTCCAATT TCAAGGTGAT GTCCTACACC AGCACAAGCC TGTCTCTGGT GTGGAGTGCC
    ACAGCAGCTG CCACCAAATT CAAAGTCACC TGGAGCCCTG TGGGAGCAGA AAAAGAAAAA
    CAGGTTGCCA AGACTCAGTA CCTGGGCAGT AGGGTGCTGG CTTACCAGAT TGACCAGTTA
    CTGCCCAACA CCCTGTATAA AGTCAGCGTT CGGGCTGTCT TCAACAAGAC TGAGGGACCA
    GAGGTGACTC TGACTCACCG CACAGCGCCT CTTGCAGACT CCAACCCCAT CCAGGCCGTT
    GAAGATCTGA GGATTACTGA CACCGGCGTA AATAGCTTGA AGCTGTCTTG GAGGAGACTT
    CCTGGGGTAA CTCATTATAA GATATCCTGG GCGCCGTTCA ATGGTGGCTT GGAAACCTCC
    CAGGTGGTTG CAGCAGAGAC GGTCTCCTTC ACGATCTCCA ACCTGAAGGA GAGCACCACC
    TACACCATCT GCATGTCTGC AATGATCGGG GGACAGGAGG GAAGCCCCAC ACTCCTCACA
    GCCAAAACTT TGGATTTACC CAAAGTGACT GAGTTCATGG TGCAGGAGGC TGCGGAGACC
    AGCGTTTTGC TGACATGGAC AGCTGTTCCT GGGGCATCCA CTTATCTATT AACATGGCGC
    CTGTCCTCAG CTTCTGATCT TTCCCAGGAA CTGCTGTCAG CAGCTTCTAG ATCGTATCGA
    GTGACAGGAC TGAGCACCAA AGAAATGTAT GTGTTTTCCA TAAGACCAGT CTTTGGAGAT
    ACGGAGGGAC CAGAGACAAC ACTCCTTGGG CAAACAGCGG GACCTCACAG AGAGTCACCC
    ACACCCATCA CTCCAGTGAC TTCTACAAAA CTGGGTACGA TGAGATTCCC ATCCTTATCT
    CCTAGCAAGG TGGGGACCAC TCCTCTTCCA CTGGCCACCA CCACAACAGT ACCACCAACT
    CCAGCAACCC TACTGTCGAC CACTGCAGTG TCCACACTGC TGACAACTCT CCCTGGGCCA
    ATCTGTGGCA AGTTCAAAGC AGACATTGGG TTCCTGGTGG ACGAGTCCTC GAGTATTGGC
    CAGAGCAATT TCAACAAAGT GAAGGATTTC CTCTTCCGGA TCATTTCCTA TTTCCCGAAG
    ATTGGGCCTG AAGGGACACA GGTTGCTGTT GCTCAGTACA GCGAGGAGCC CAGGGCAGCC
    TTCTACTTCA ACCAGCACCG GGACCGCAAC AGTGCCCTGA AAGCCGTCAA GGGACTGAGC
    TACGCTGGGG GCAACACAAA AACAGGTCGT GGCATAACCT ACATGCTCAA GGAGTTATTT
    CAGTCCTCCA GAGGGATGAG GCCAGATTTC CCTCACGTGC TGGTGCTGGT GACCGATGGG
    CGATCCCAGG ATGATGTGTT GCTACCAGCC AGAGCAGCCC ATGAGTTGGG GATCCGCGTC
    ATTGCTGTGG GTGTCTCAGG AGCTGACCCC GTTGAGCTCA ACAGCATCTT GCTGCAGCAG
    AACCTCCAGA ATGTTTTCTA CGTCAGCACG TTTGATGACT TCCCCCAGAT CCTCCGAGAG
    CTGATAGAGG TCATCTGTTC TGACCCCGAG CCTTCAGGGC CTCAACTACC ATCTGGGGAG
    AGCTTCAGGA GTTCACCAGC TCCTCATGGA GGCTATGACC CATACAGCTT TACTACAAAG
    GGAGAGAAAG GAGAGAGGGG TCTACCTGGG AAGGATGGAA TTCCCGGGCT GCCGGGCAGA
    CCGGGGCGGA CAGGTCCTCC TGGTCCCCCT GGACTCATGG GTCTCCCTGG CATCCAAGGT
    GATACTGGCT CTCCTGGCTA TCCTGGACCA GCGGGGCCAA AAGGAGAGAG AGGCGAACCA
    GGCTATGTCC TGGGAGGAGT AGAGGTGATT CCTGGCCGAA ATGGGCAGCC TGGCCCCCCT
    GGACAGAAAG GGCAGCCGGG GGTTCCTGGG GTAGCAGGAC CACCAGGTTT ACCAGGGCCA
    CAGGGGCCAC CAGGCATCTC CATCAAGGGT GAACGTGGGG ATTCAGGTAC CAGGGGCCCT
    CGTGGGAGGA ATGGCCTCAA GGGTGACAGA GGAGGAATGG GAGAGCCGGG GAAACCTGGG
    TTGCCAGGCC CAGTAGGGCT AGATGGTGTT CCTGGATTGC CTGGACCAAG GGGAGAAAAG
    GGTGTGATGG GACCTCCAGG AGCACCAGGA CCAAAGGGTA ACAAAGGGCA AGGTGGTGTG
    AAAGGGGAAA AGGGACTCCC AGGACCTCCA GGGCAGAAGG GAGACCAGGG GAACCCAGGA
    TTTCCAGGTG CTCCAGCTAT GGGGGTTTTG GGACCTCCAG GCAAGAAAGG TGTCAGGGGT
    GATATTGGAC CAACAGGGGC CCTGGGACCA CAAGGAGACA AAGGAATACA AGGAGACAAA
    GGAGAGAAGG GAAGCCCAGG TTTCGGGATT CCTGGTCAAC CTGGGCTGAA GGGAGATCCA
    GGCGACAGGG GAAATGTAGG TTTGTCGGGC AAACCAGGTC AGAAGGGGGA TAGAGGTCTG
    AAGGGAGAGA AAGGTGAACC AGGACTACCT GGGAAACCAG GCGAGACCGG ACTAAGAGGT
    AAAGATGGAG AACCAGGGGA GAAAGGCACC TCTGGGACCA AGGGAGAAGC TGGTGTCCCT
    GGAGAACCAG GAGAGAGAGG AATCAGGGGG CCACCTGGAC TCCCAGGACG GCCTGGAGAG
    CAAGGGATAA AAGGCGATAC AGGACCACCT GGAAAGGATG GAATCACTGG AGAGAAGGGA
    GACAAGGGCG AAGCTGGTGA GCCTGGGCTG CCTGGGACTC CTGGAAGCCT TGTGTCTTCA
    CTGGAGAGCA CCATCACCCT GAAAGGCGAC AAGGGGGATC GTGGGAAGGA TGGCTTGAAA
    GGCGAAAGAG GTCCTCCAGG CCCAAAGGGA GAACCCGGTC CACCAGGCAA AGGAGTGGAA
    ATGAAGGATC TGGAGAGGCT TTTTGAAGCA AATGGTATCA AGCTGGCACT GCTGAAGGAC
    CTCTCCAATG CACTCCTGCA GGATGGGCTG GATGGGCTGG TGTGGCAGCT GGGTGGCTCC
    AGGACAAGCA AGACCTCCAG GAGAAAGCAA GCAGTTCTTC ATGCTACTGA GCATGGCGAT
    TCACTGGTGT TTGATACTTC TCCTGGTGCC CTGGCCAGCA CTGAGGTGAC AGAAGAAGCA
    CAGAGCCTGG AGGTGGAGGC TCAGTTTAGG GTGCCGGTGT CTGGAGGACT CCTTAAGGGT
    CCATCTGCTG GTGGTTCTGA ACCTGAGTAC CAGCCCCTAG GAAACCCTCC TGAGTCCTTG
    GAGAGAACAT CTGAGGAAGG AAAGGAGGAG AGAGACCCAA GATGGACAAT CACTTCTCTC
    AGTGGTAGCT TGGCTTTGCC CCCACTGCAC CCAGAAGATG ATCTGCAAGG AAACCAGTCC
    ATGGAGCTTC TTGAGTCCCT GGAGGGGAGA GTGGAAGGAC AACCACAGCA GGACACTAGC
    CACGAAAATG AGAATTTAGA CGACGGCTCT GCGCTCCCAA GGCACGTTAG AGCCCAGAGA
    TCTGCTGAGG ACCAGAGACA CGTGGTAAAG ACCCCATTTC ACCACGACAG CTTCGGGCTG
    GCCTTTGTGC AAGAGCAGAG CCATCCGTGT GACCCAGGAG GAAACGGCGA CCTTTCCTTG
    TTGGCTCCAT CACAGGTTCA TCCCCCTGGT GCTCCAGGAG CTCGGAAATC CCCATCTGGA
    TCCACGGCAT ACCCAGGCCC TCATCACCCA AGGAGAATTA AGAAGGAGGA AGCTGGTCCA
    CCAGATCCAC CATCCCATGG CCAAAAGGGA GAAATGGGAG CTCCGGGAGT CCGTGGAGAC
    AAAGGAGAAA AGGGGCAGCC TGGGGAGATG GGGGAACCTG GAGAAAAGGG CATCAAAGGA
    GACAGAGGTG AAAATGGTCA AAAGGGAGAA CCAGGAATTG GGTTCAGAGG CCCTGTTGGT
    CAGGCTGGAC CACCTGGATT TAAAGGTGAG CCAGGGGCTC CAGGGCCTCC AGGAGCTCAG
    GGAGTCCAGG GAATTCGTGG CAATGCCGGC ACCCCCGGGT CACAAGGGGA AAGAGGGGCT
    CCAGGTCTGC CTGGGATGCC AGGACAAAAG GGAGAGCGTG GGAAGAGAGG ACGGAATGGA
    TTTGCTGGGC CCTCAGGACC ATCAGGATCC CCAGGAAAAG AGGGGATTTC TGGGACACCT
    GGACCCAAAG GCAACAAGGG AGAAATCGGC GTTGGAGCAG CTGGCCCAAG AGGTCCTCGT
    GGACTGCCTG GCCCTCGGGG TGACGAGGGC ATCGTGGGAG TCCGTGGCCC CACCGGCATG
    ACGGGTCTAA AAGGCTTCCC GGGTGTGAAG GGCGAAAGAG GTGATCGGGG ACTTCTGGGA
    CCCAAAGGAG AAAGAGGTGA GCCCATGACT ATTTTTGGAC CCCAAGGCTA TAAAGGCAGT
    AAAGGAGATC CTGGTGAACG GGGCCTGCCA GGTTTTGACG GAGACAAAGG CGAGAAAGGA
    GAGGATGGGC CTATAGGAGA AAAGGGAGTC AAAGGTGAAG CTGGCTCCAA GGGAGTGATG
    GGGCTTTTTG GAACAAGAGG ACCAGTGGGA CAGAAGGGGG AGCCAGGAGA ACCAGGCTTG
    CCGGGCTCTG CTGGTGCAGC AGGTCTTAAT GGGAAGAACG GAAACAAGGG AGCCAAAGGT
    GACAGAGGTC TTCAGGGGCA GAAGGGGAAG CCGGGAGGAA AAGGTGATCC TGGGGCCACT
    GGTGACATTG GACAGAAAGG AACAAAGGGC TTACGAGGGC TTCCAGGACG CATCGGTGCT
    CCTGGAGCTG AAGGCATGAA GGGAGAAATT GGCAGTCCTG GAAAACCAGG AATCCCAGGA
    TCAGATGGAC CACACGGACA AAAGGGTGAA AAGGGAACAT CAGGAGACGA TGGTGCCTCG
    GGGATTCCTG GGGAGAAAGG AGAAAAGGGA GCCAAAGGAG TTCCTGGCTT GGGAGGCTTC
    AAAGGACAAA TGGGATGGCC TGGGAGGACT GGAGTTGCTG GACCAGATGG CCCTCAAGGG
    CCACAGGGTG AACCAGGGCC CCAGGGTCCA CGAGGGAAAA GAGGGAGACC TCAGCTCTGC
    CTCAGGGGAC CCCCAGGCAT GGATGGGAGA AAAGGAGAAA TGGGTGAAAA TGGTCCAGCC
    GGCCAGAAAG GAGAGAAGGG AGATCCTGGT CTTTCTGAGG AAGAGGTGAA GGAGGTGGTC
    AGGAGCGAAA TGAGAGGCAG ATGCGATTGT GGAGGCGAGT TGGGAAGGAC ACTCATGGAA
    AGGGGCCTGG AGGCAACTGA AATCAACAGG CAGCTCCAAG GGTTCCAAAA GTTCCAGCTT
    TGGGAACGCA CCAGAAGGGA TGGTGAAAGC CAGGAAGAAA TGCTGAACAA AACCCAGGGG
    ATGACCATGT TGATGACAAT GGTGAAGCCC GTCATGGAGC CACCTTCACT TATTGCAGCG
    GCTCCCCGGG CGCAGCAGAT AACAGGTCCC TCTCAGCGCA AGCGGCGAGA AGCCCACAGT
    CAATTACCAG CCCCCTGCCT GCAGCCGATG GATGAAGGCT CCTGCCAGCA CTATGCCCTG
    CTCTGGTATT ACCACCTGGA GGCAAACACC TGCCGGCCCT TCATCTTTGG GGGATGCCGA
    GGGAACAGCA ACCGCTTCGA AACCAAGTGG AAGTGTGAGC AGAAGTGCAA AACCTCAGCA
    GCCAGAGGGA GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa78934
    Clone ID Related Accession (Same CDS sequence) XM_014961016.1 , XM_014961017.1 , XM_014961015.1
    Accession Version XM_014961017.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7515bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product collagen alpha-1(VII) chain-like isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTTTGTG CCCAGATCCT TGCCCTGGCA GTGGCTGTGG TGCAGCACAG AGCTGTGGCC 
    GTTCATGAAG CATGCAGGAC GGCGGAGGTG GCCGACATTC TGTTCCTCGT GGGACAGTCA
    CAGGGCACAG GAAGGGAGAG CTCCTGGCTG GTCAAGGACT TCATCAGCAG CATGGCTCGT
    TCCTTTGAGA ACGTGGTGAT GGGCAAAGGA GGCATCCGGC TGGCAGTGGC ACTCTACGGG
    GAGAAACCAA GGATGAGTAT TGAGCTTACG GATTACGTGA CCATCGAAGA AATGTTGGTG
    GCAATTCAGG ATCTCTCCTT CAAAGGTGGA AGCTTAAAAA CAGGGTCAGC CTTGGCCTTT
    GCAGCTCGTG CCATGTCTCG TCTGGACACA CTGCGAGAGG ATGCAGCAAA GGTGGTTGTT
    TTGATCACGG ATGGAAAATC CAGCAACTCA GTTGAAGATG AAGCCCGGGT CCTGCAGGAT
    TTGGGGGTGA CGGTGTTTGC CGTAGGGATT AAGGGTGCTG ACAAGAATGA ATTGAACACA
    ATAGCTTCAG AGCCCACTGC AGAGCATGTG CTTTATGTTG AAGATTTCCA CCTCCTCCAT
    AACGTGGCCC CCAAACTCTC TCGAAGGCTC TGTTTCACTG CCTCGGAGCC ACCACGACCC
    ATCAAACAGA CTGTGCAAGC TGAGAAGATC GTCGGCCCCC GGGATTTGTA TGTCAGTGAG
    CACAGCCACA GCACGCTGAG ACTCACCTGG ATGCCAGCCA CGGGGAAGGT GACGGGCTAC
    CATGTCCATC TGCATCCCTT GCTGCCTTCA GGGCAGCCCG TTTCAGAAGA CCAGCAGCAG
    ATTGTGGTGG ACGGTGACAA AAGTACCGTG CTGGTGACTG ATCTGAAACC CAACACCAAG
    TATGTCTTCA CGGTGAGGGC CATCTATGCA GATACTGTTG GGGAGTCAGC GGTTGTCAAG
    GGGAAAACAA CACCTGTGCC TCCAGTCACT AATTTCCGTG TGATAGAAGA AGGACTTTTT
    AGCTTGAAGG TGGCTTGGAC ACCTCCGCTT GGCAAGCTGG AGGGCTACAA GATCTACATC
    CCCAGAGCCA ACAGACCAGG AATGACCTAC GAGCAGGTTC TGCGGGGTGA TGTCTCATCC
    CACGTGCTGG AGAACTTGCA GGAGGACAGA GAATACAGCA TCAGCATCTA CGCCGTGTAC
    CCCCAGGGCC CCAGCCAGCC TGTGGCTGCT GTGGGGAGAA CATTGAAGCT CCTGCCCGTG
    AAAAGCATCT TGCTGCAGAA TGAGACCACC AACACGCTCC AGGCAAGGTG GACACCCATC
    CGGGGAGCAA CGGGGTACAG GCTCACCTGG ACATCAGCAG AGGGCAGCAT CCAAGATGTG
    AACCTCAGCA ACACCTACAG CTACTACATG ATCCAGGGGC TGCAGCCTGG CACGGAGTAC
    ACTGTTACCA TCAACCCCAT CTTTGGGGAC GTCGAGGGAC CAGTGGTGAG CAGCAAAGCA
    ACGACGGTGG CATCGAGCAC CGTCCAGATG CTGAAAGTGA CTGAGATATC TACCAACAGT
    GCTCTGGTCT CCTGGGACTC AGTTGCTGGG GCCACTGGGT ATCGAGTGGC TTGGGGTCCT
    ACACCAGAGT TCTTTGGCAA AGACCGTCCT CGCCAGCTGG CCCTCAACAG GTCAATCACA
    GCCTACCAGC TCCGCAACCT GGCCCATGAC ACCGAGTACG TGATTTCCCT CTACGTGCTC
    TTTGGCTCCG TGGAAGGACC AGGGATCACA ACCTCAGCCA GGACATCTCC TCTTGGCTAC
    GTCTCCAATT TCAAGGTGAT GTCCTACACC AGCACAAGCC TGTCTCTGGT GTGGAGTGCC
    ACAGCAGCTG CCACCAAATT CAAAGTCACC TGGAGCCCTG TGGGAGCAGA AAAAGAAAAA
    CAGGTTGCCA AGACTCAGTA CCTGGGCAGT AGGGTGCTGG CTTACCAGAT TGACCAGTTA
    CTGCCCAACA CCCTGTATAA AGTCAGCGTT CGGGCTGTCT TCAACAAGAC TGAGGGACCA
    GAGGTGACTC TGACTCACCG CACAGCGCCT CTTGCAGACT CCAACCCCAT CCAGGCCGTT
    GAAGATCTGA GGATTACTGA CACCGGCGTA AATAGCTTGA AGCTGTCTTG GAGGAGACTT
    CCTGGGGTAA CTCATTATAA GATATCCTGG GCGCCGTTCA ATGGTGGCTT GGAAACCTCC
    CAGGTGGTTG CAGCAGAGAC GGTCTCCTTC ACGATCTCCA ACCTGAAGGA GAGCACCACC
    TACACCATCT GCATGTCTGC AATGATCGGG GGACAGGAGG GAAGCCCCAC ACTCCTCACA
    GCCAAAACTT TGGATTTACC CAAAGTGACT GAGTTCATGG TGCAGGAGGC TGCGGAGACC
    AGCGTTTTGC TGACATGGAC AGCTGTTCCT GGGGCATCCA CTTATCTATT AACATGGCGC
    CTGTCCTCAG CTTCTGATCT TTCCCAGGAA CTGCTGTCAG CAGCTTCTAG ATCGTATCGA
    GTGACAGGAC TGAGCACCAA AGAAATGTAT GTGTTTTCCA TAAGACCAGT CTTTGGAGAT
    ACGGAGGGAC CAGAGACAAC ACTCCTTGGG CAAACAGCGG GACCTCACAG AGAGTCACCC
    ACACCCATCA CTCCAGTGAC TTCTACAAAA CTGGGTACGA TGAGATTCCC ATCCTTATCT
    CCTAGCAAGG TGGGGACCAC TCCTCTTCCA CTGGCCACCA CCACAACAGT ACCACCAACT
    CCAGCAACCC TACTGTCGAC CACTGCAGTG TCCACACTGC TGACAACTCT CCCTGGGCCA
    ATCTGTGGCA AGTTCAAAGC AGACATTGGG TTCCTGGTGG ACGAGTCCTC GAGTATTGGC
    CAGAGCAATT TCAACAAAGT GAAGGATTTC CTCTTCCGGA TCATTTCCTA TTTCCCGAAG
    ATTGGGCCTG AAGGGACACA GGTTGCTGTT GCTCAGTACA GCGAGGAGCC CAGGGCAGCC
    TTCTACTTCA ACCAGCACCG GGACCGCAAC AGTGCCCTGA AAGCCGTCAA GGGACTGAGC
    TACGCTGGGG GCAACACAAA AACAGGTCGT GGCATAACCT ACATGCTCAA GGAGTTATTT
    CAGTCCTCCA GAGGGATGAG GCCAGATTTC CCTCACGTGC TGGTGCTGGT GACCGATGGG
    CGATCCCAGG ATGATGTGTT GCTACCAGCC AGAGCAGCCC ATGAGTTGGG GATCCGCGTC
    ATTGCTGTGG GTGTCTCAGG AGCTGACCCC GTTGAGCTCA ACAGCATCTT GCTGCAGCAG
    AACCTCCAGA ATGTTTTCTA CGTCAGCACG TTTGATGACT TCCCCCAGAT CCTCCGAGAG
    CTGATAGAGG TCATCTGTTC TGACCCCGAG CCTTCAGGGC CTCAACTACC ATCTGGGGAG
    AGCTTCAGGA GTTCACCAGC TCCTCATGGA GGCTATGACC CATACAGCTT TACTACAAAG
    GGAGAGAAAG GAGAGAGGGG TCTACCTGGG AAGGATGGAA TTCCCGGGCT GCCGGGCAGA
    CCGGGGCGGA CAGGTCCTCC TGGTCCCCCT GGACTCATGG GTCTCCCTGG CATCCAAGGT
    GATACTGGCT CTCCTGGCTA TCCTGGACCA GCGGGGCCAA AAGGAGAGAG AGGCGAACCA
    GGCTATGTCC TGGGAGGAGT AGAGGTGATT CCTGGCCGAA ATGGGCAGCC TGGCCCCCCT
    GGACAGAAAG GGCAGCCGGG GGTTCCTGGG GTAGCAGGAC CACCAGGTTT ACCAGGGCCA
    CAGGGGCCAC CAGGCATCTC CATCAAGGGT GAACGTGGGG ATTCAGGTAC CAGGGGCCCT
    CGTGGGAGGA ATGGCCTCAA GGGTGACAGA GGAGGAATGG GAGAGCCGGG GAAACCTGGG
    TTGCCAGGCC CAGTAGGGCT AGATGGTGTT CCTGGATTGC CTGGACCAAG GGGAGAAAAG
    GGTGTGATGG GACCTCCAGG AGCACCAGGA CCAAAGGGTA ACAAAGGGCA AGGTGGTGTG
    AAAGGGGAAA AGGGACTCCC AGGACCTCCA GGGCAGAAGG GAGACCAGGG GAACCCAGGA
    TTTCCAGGTG CTCCAGCTAT GGGGGTTTTG GGACCTCCAG GCAAGAAAGG TGTCAGGGGT
    GATATTGGAC CAACAGGGGC CCTGGGACCA CAAGGAGACA AAGGAATACA AGGAGACAAA
    GGAGAGAAGG GAAGCCCAGG TTTCGGGATT CCTGGTCAAC CTGGGCTGAA GGGAGATCCA
    GGCGACAGGG GAAATGTAGG TTTGTCGGGC AAACCAGGTC AGAAGGGGGA TAGAGGTCTG
    AAGGGAGAGA AAGGTGAACC AGGACTACCT GGGAAACCAG GCGAGACCGG ACTAAGAGGT
    AAAGATGGAG AACCAGGGGA GAAAGGCACC TCTGGGACCA AGGGAGAAGC TGGTGTCCCT
    GGAGAACCAG GAGAGAGAGG AATCAGGGGG CCACCTGGAC TCCCAGGACG GCCTGGAGAG
    CAAGGGATAA AAGGCGATAC AGGACCACCT GGAAAGGATG GAATCACTGG AGAGAAGGGA
    GACAAGGGCG AAGCTGGTGA GCCTGGGCTG CCTGGGACTC CTGGAAGCCT TGTGTCTTCA
    CTGGAGAGCA CCATCACCCT GAAAGGCGAC AAGGGGGATC GTGGGAAGGA TGGCTTGAAA
    GGCGAAAGAG GTCCTCCAGG CCCAAAGGGA GAACCCGGTC CACCAGGCAA AGGAGTGGAA
    ATGAAGGATC TGGAGAGGCT TTTTGAAGCA AATGGTATCA AGCTGGCACT GCTGAAGGAC
    CTCTCCAATG CACTCCTGCA GGATGGGCTG GATGGGCTGG TGTGGCAGCT GGGTGGCTCC
    AGGACAAGCA AGACCTCCAG GAGAAAGCAA GCAGTTCTTC ATGCTACTGA GCATGGCGAT
    TCACTGGTGT TTGATACTTC TCCTGGTGCC CTGGCCAGCA CTGAGGTGAC AGAAGAAGCA
    CAGAGCCTGG AGGTGGAGGC TCAGTTTAGG GTGCCGGTGT CTGGAGGACT CCTTAAGGGT
    CCATCTGCTG GTGGTTCTGA ACCTGAGTAC CAGCCCCTAG GAAACCCTCC TGAGTCCTTG
    GAGAGAACAT CTGAGGAAGG AAAGGAGGAG AGAGACCCAA GATGGACAAT CACTTCTCTC
    AGTGGTAGCT TGGCTTTGCC CCCACTGCAC CCAGAAGATG ATCTGCAAGG AAACCAGTCC
    ATGGAGCTTC TTGAGTCCCT GGAGGGGAGA GTGGAAGGAC AACCACAGCA GGACACTAGC
    CACGAAAATG AGAATTTAGA CGACGGCTCT GCGCTCCCAA GGCACGTTAG AGCCCAGAGA
    TCTGCTGAGG ACCAGAGACA CGTGGTAAAG ACCCCATTTC ACCACGACAG CTTCGGGCTG
    GCCTTTGTGC AAGAGCAGAG CCATCCGTGT GACCCAGGAG GAAACGGCGA CCTTTCCTTG
    TTGGCTCCAT CACAGGTTCA TCCCCCTGGT GCTCCAGGAG CTCGGAAATC CCCATCTGGA
    TCCACGGCAT ACCCAGGCCC TCATCACCCA AGGAGAATTA AGAAGGAGGA AGCTGGTCCA
    CCAGATCCAC CATCCCATGG CCAAAAGGGA GAAATGGGAG CTCCGGGAGT CCGTGGAGAC
    AAAGGAGAAA AGGGGCAGCC TGGGGAGATG GGGGAACCTG GAGAAAAGGG CATCAAAGGA
    GACAGAGGTG AAAATGGTCA AAAGGGAGAA CCAGGAATTG GGTTCAGAGG CCCTGTTGGT
    CAGGCTGGAC CACCTGGATT TAAAGGTGAG CCAGGGGCTC CAGGGCCTCC AGGAGCTCAG
    GGAGTCCAGG GAATTCGTGG CAATGCCGGC ACCCCCGGGT CACAAGGGGA AAGAGGGGCT
    CCAGGTCTGC CTGGGATGCC AGGACAAAAG GGAGAGCGTG GGAAGAGAGG ACGGAATGGA
    TTTGCTGGGC CCTCAGGACC ATCAGGATCC CCAGGAAAAG AGGGGATTTC TGGGACACCT
    GGACCCAAAG GCAACAAGGG AGAAATCGGC GTTGGAGCAG CTGGCCCAAG AGGTCCTCGT
    GGACTGCCTG GCCCTCGGGG TGACGAGGGC ATCGTGGGAG TCCGTGGCCC CACCGGCATG
    ACGGGTCTAA AAGGCTTCCC GGGTGTGAAG GGCGAAAGAG GTGATCGGGG ACTTCTGGGA
    CCCAAAGGAG AAAGAGGTGA GCCCATGACT ATTTTTGGAC CCCAAGGCTA TAAAGGCAGT
    AAAGGAGATC CTGGTGAACG GGGCCTGCCA GGTTTTGACG GAGACAAAGG CGAGAAAGGA
    GAGGATGGGC CTATAGGAGA AAAGGGAGTC AAAGGTGAAG CTGGCTCCAA GGGAGTGATG
    GGGCTTTTTG GAACAAGAGG ACCAGTGGGA CAGAAGGGGG AGCCAGGAGA ACCAGGCTTG
    CCGGGCTCTG CTGGTGCAGC AGGTCTTAAT GGGAAGAACG GAAACAAGGG AGCCAAAGGT
    GACAGAGGTC TTCAGGGGCA GAAGGGGAAG CCGGGAGGAA AAGGTGATCC TGGGGCCACT
    GGTGACATTG GACAGAAAGG AACAAAGGGC TTACGAGGGC TTCCAGGACG CATCGGTGCT
    CCTGGAGCTG AAGGCATGAA GGGAGAAATT GGCAGTCCTG GAAAACCAGG AATCCCAGGA
    TCAGATGGAC CACACGGACA AAAGGGTGAA AAGGGAACAT CAGGAGACGA TGGTGCCTCG
    GGGATTCCTG GGGAGAAAGG AGAAAAGGGA GCCAAAGGAG TTCCTGGCTT GGGAGGCTTC
    AAAGGACAAA TGGGATGGCC TGGGAGGACT GGAGTTGCTG GACCAGATGG CCCTCAAGGG
    CCACAGGGTG AACCAGGGCC CCAGGGTCCA CGAGGGAAAA GAGGGAGACC TCAGCTCTGC
    CTCAGGGGAC CCCCAGGCAT GGATGGGAGA AAAGGAGAAA TGGGTGAAAA TGGTCCAGCC
    GGCCAGAAAG GAGAGAAGGG AGATCCTGGT CTTTCTGAGG AAGAGGTGAA GGAGGTGGTC
    AGGAGCGAAA TGAGAGGCAG ATGCGATTGT GGAGGCGAGT TGGGAAGGAC ACTCATGGAA
    AGGGGCCTGG AGGCAACTGA AATCAACAGG CAGCTCCAAG GGTTCCAAAA GTTCCAGCTT
    TGGGAACGCA CCAGAAGGGA TGGTGAAAGC CAGGAAGAAA TGCTGAACAA AACCCAGGGG
    ATGACCATGT TGATGACAAT GGTGAAGCCC GTCATGGAGC CACCTTCACT TATTGCAGCG
    GCTCCCCGGG CGCAGCAGAT AACAGGTCCC TCTCAGCGCA AGCGGCGAGA AGCCCACAGT
    CAATTACCAG CCCCCTGCCT GCAGCCGATG GATGAAGGCT CCTGCCAGCA CTATGCCCTG
    CTCTGGTATT ACCACCTGGA GGCAAACACC TGCCGGCCCT TCATCTTTGG GGGATGCCGA
    GGGAACAGCA ACCGCTTCGA AACCAAGTGG AAGTGTGAGC AGAAGTGCAA AACCTCAGCA
    GCCAGAGGGA GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014816503.1
    CDS249..7763
    Translation

    Target ORF information:

    RefSeq Version XM_014961017.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax collagen alpha-1(VII) chain-like (LOC106899270), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014961017.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTTTGTG CCCAGATCCT TGCCCTGGCA GTGGCTGTGG TGCAGCACAG AGCTGTGGCC 
    GTTCATGAAG CATGCAGGAC GGCGGAGGTG GCCGACATTC TGTTCCTCGT GGGACAGTCA
    CAGGGCACAG GAAGGGAGAG CTCCTGGCTG GTCAAGGACT TCATCAGCAG CATGGCTCGT
    TCCTTTGAGA ACGTGGTGAT GGGCAAAGGA GGCATCCGGC TGGCAGTGGC ACTCTACGGG
    GAGAAACCAA GGATGAGTAT TGAGCTTACG GATTACGTGA CCATCGAAGA AATGTTGGTG
    GCAATTCAGG ATCTCTCCTT CAAAGGTGGA AGCTTAAAAA CAGGGTCAGC CTTGGCCTTT
    GCAGCTCGTG CCATGTCTCG TCTGGACACA CTGCGAGAGG ATGCAGCAAA GGTGGTTGTT
    TTGATCACGG ATGGAAAATC CAGCAACTCA GTTGAAGATG AAGCCCGGGT CCTGCAGGAT
    TTGGGGGTGA CGGTGTTTGC CGTAGGGATT AAGGGTGCTG ACAAGAATGA ATTGAACACA
    ATAGCTTCAG AGCCCACTGC AGAGCATGTG CTTTATGTTG AAGATTTCCA CCTCCTCCAT
    AACGTGGCCC CCAAACTCTC TCGAAGGCTC TGTTTCACTG CCTCGGAGCC ACCACGACCC
    ATCAAACAGA CTGTGCAAGC TGAGAAGATC GTCGGCCCCC GGGATTTGTA TGTCAGTGAG
    CACAGCCACA GCACGCTGAG ACTCACCTGG ATGCCAGCCA CGGGGAAGGT GACGGGCTAC
    CATGTCCATC TGCATCCCTT GCTGCCTTCA GGGCAGCCCG TTTCAGAAGA CCAGCAGCAG
    ATTGTGGTGG ACGGTGACAA AAGTACCGTG CTGGTGACTG ATCTGAAACC CAACACCAAG
    TATGTCTTCA CGGTGAGGGC CATCTATGCA GATACTGTTG GGGAGTCAGC GGTTGTCAAG
    GGGAAAACAA CACCTGTGCC TCCAGTCACT AATTTCCGTG TGATAGAAGA AGGACTTTTT
    AGCTTGAAGG TGGCTTGGAC ACCTCCGCTT GGCAAGCTGG AGGGCTACAA GATCTACATC
    CCCAGAGCCA ACAGACCAGG AATGACCTAC GAGCAGGTTC TGCGGGGTGA TGTCTCATCC
    CACGTGCTGG AGAACTTGCA GGAGGACAGA GAATACAGCA TCAGCATCTA CGCCGTGTAC
    CCCCAGGGCC CCAGCCAGCC TGTGGCTGCT GTGGGGAGAA CATTGAAGCT CCTGCCCGTG
    AAAAGCATCT TGCTGCAGAA TGAGACCACC AACACGCTCC AGGCAAGGTG GACACCCATC
    CGGGGAGCAA CGGGGTACAG GCTCACCTGG ACATCAGCAG AGGGCAGCAT CCAAGATGTG
    AACCTCAGCA ACACCTACAG CTACTACATG ATCCAGGGGC TGCAGCCTGG CACGGAGTAC
    ACTGTTACCA TCAACCCCAT CTTTGGGGAC GTCGAGGGAC CAGTGGTGAG CAGCAAAGCA
    ACGACGGTGG CATCGAGCAC CGTCCAGATG CTGAAAGTGA CTGAGATATC TACCAACAGT
    GCTCTGGTCT CCTGGGACTC AGTTGCTGGG GCCACTGGGT ATCGAGTGGC TTGGGGTCCT
    ACACCAGAGT TCTTTGGCAA AGACCGTCCT CGCCAGCTGG CCCTCAACAG GTCAATCACA
    GCCTACCAGC TCCGCAACCT GGCCCATGAC ACCGAGTACG TGATTTCCCT CTACGTGCTC
    TTTGGCTCCG TGGAAGGACC AGGGATCACA ACCTCAGCCA GGACATCTCC TCTTGGCTAC
    GTCTCCAATT TCAAGGTGAT GTCCTACACC AGCACAAGCC TGTCTCTGGT GTGGAGTGCC
    ACAGCAGCTG CCACCAAATT CAAAGTCACC TGGAGCCCTG TGGGAGCAGA AAAAGAAAAA
    CAGGTTGCCA AGACTCAGTA CCTGGGCAGT AGGGTGCTGG CTTACCAGAT TGACCAGTTA
    CTGCCCAACA CCCTGTATAA AGTCAGCGTT CGGGCTGTCT TCAACAAGAC TGAGGGACCA
    GAGGTGACTC TGACTCACCG CACAGCGCCT CTTGCAGACT CCAACCCCAT CCAGGCCGTT
    GAAGATCTGA GGATTACTGA CACCGGCGTA AATAGCTTGA AGCTGTCTTG GAGGAGACTT
    CCTGGGGTAA CTCATTATAA GATATCCTGG GCGCCGTTCA ATGGTGGCTT GGAAACCTCC
    CAGGTGGTTG CAGCAGAGAC GGTCTCCTTC ACGATCTCCA ACCTGAAGGA GAGCACCACC
    TACACCATCT GCATGTCTGC AATGATCGGG GGACAGGAGG GAAGCCCCAC ACTCCTCACA
    GCCAAAACTT TGGATTTACC CAAAGTGACT GAGTTCATGG TGCAGGAGGC TGCGGAGACC
    AGCGTTTTGC TGACATGGAC AGCTGTTCCT GGGGCATCCA CTTATCTATT AACATGGCGC
    CTGTCCTCAG CTTCTGATCT TTCCCAGGAA CTGCTGTCAG CAGCTTCTAG ATCGTATCGA
    GTGACAGGAC TGAGCACCAA AGAAATGTAT GTGTTTTCCA TAAGACCAGT CTTTGGAGAT
    ACGGAGGGAC CAGAGACAAC ACTCCTTGGG CAAACAGCGG GACCTCACAG AGAGTCACCC
    ACACCCATCA CTCCAGTGAC TTCTACAAAA CTGGGTACGA TGAGATTCCC ATCCTTATCT
    CCTAGCAAGG TGGGGACCAC TCCTCTTCCA CTGGCCACCA CCACAACAGT ACCACCAACT
    CCAGCAACCC TACTGTCGAC CACTGCAGTG TCCACACTGC TGACAACTCT CCCTGGGCCA
    ATCTGTGGCA AGTTCAAAGC AGACATTGGG TTCCTGGTGG ACGAGTCCTC GAGTATTGGC
    CAGAGCAATT TCAACAAAGT GAAGGATTTC CTCTTCCGGA TCATTTCCTA TTTCCCGAAG
    ATTGGGCCTG AAGGGACACA GGTTGCTGTT GCTCAGTACA GCGAGGAGCC CAGGGCAGCC
    TTCTACTTCA ACCAGCACCG GGACCGCAAC AGTGCCCTGA AAGCCGTCAA GGGACTGAGC
    TACGCTGGGG GCAACACAAA AACAGGTCGT GGCATAACCT ACATGCTCAA GGAGTTATTT
    CAGTCCTCCA GAGGGATGAG GCCAGATTTC CCTCACGTGC TGGTGCTGGT GACCGATGGG
    CGATCCCAGG ATGATGTGTT GCTACCAGCC AGAGCAGCCC ATGAGTTGGG GATCCGCGTC
    ATTGCTGTGG GTGTCTCAGG AGCTGACCCC GTTGAGCTCA ACAGCATCTT GCTGCAGCAG
    AACCTCCAGA ATGTTTTCTA CGTCAGCACG TTTGATGACT TCCCCCAGAT CCTCCGAGAG
    CTGATAGAGG TCATCTGTTC TGACCCCGAG CCTTCAGGGC CTCAACTACC ATCTGGGGAG
    AGCTTCAGGA GTTCACCAGC TCCTCATGGA GGCTATGACC CATACAGCTT TACTACAAAG
    GGAGAGAAAG GAGAGAGGGG TCTACCTGGG AAGGATGGAA TTCCCGGGCT GCCGGGCAGA
    CCGGGGCGGA CAGGTCCTCC TGGTCCCCCT GGACTCATGG GTCTCCCTGG CATCCAAGGT
    GATACTGGCT CTCCTGGCTA TCCTGGACCA GCGGGGCCAA AAGGAGAGAG AGGCGAACCA
    GGCTATGTCC TGGGAGGAGT AGAGGTGATT CCTGGCCGAA ATGGGCAGCC TGGCCCCCCT
    GGACAGAAAG GGCAGCCGGG GGTTCCTGGG GTAGCAGGAC CACCAGGTTT ACCAGGGCCA
    CAGGGGCCAC CAGGCATCTC CATCAAGGGT GAACGTGGGG ATTCAGGTAC CAGGGGCCCT
    CGTGGGAGGA ATGGCCTCAA GGGTGACAGA GGAGGAATGG GAGAGCCGGG GAAACCTGGG
    TTGCCAGGCC CAGTAGGGCT AGATGGTGTT CCTGGATTGC CTGGACCAAG GGGAGAAAAG
    GGTGTGATGG GACCTCCAGG AGCACCAGGA CCAAAGGGTA ACAAAGGGCA AGGTGGTGTG
    AAAGGGGAAA AGGGACTCCC AGGACCTCCA GGGCAGAAGG GAGACCAGGG GAACCCAGGA
    TTTCCAGGTG CTCCAGCTAT GGGGGTTTTG GGACCTCCAG GCAAGAAAGG TGTCAGGGGT
    GATATTGGAC CAACAGGGGC CCTGGGACCA CAAGGAGACA AAGGAATACA AGGAGACAAA
    GGAGAGAAGG GAAGCCCAGG TTTCGGGATT CCTGGTCAAC CTGGGCTGAA GGGAGATCCA
    GGCGACAGGG GAAATGTAGG TTTGTCGGGC AAACCAGGTC AGAAGGGGGA TAGAGGTCTG
    AAGGGAGAGA AAGGTGAACC AGGACTACCT GGGAAACCAG GCGAGACCGG ACTAAGAGGT
    AAAGATGGAG AACCAGGGGA GAAAGGCACC TCTGGGACCA AGGGAGAAGC TGGTGTCCCT
    GGAGAACCAG GAGAGAGAGG AATCAGGGGG CCACCTGGAC TCCCAGGACG GCCTGGAGAG
    CAAGGGATAA AAGGCGATAC AGGACCACCT GGAAAGGATG GAATCACTGG AGAGAAGGGA
    GACAAGGGCG AAGCTGGTGA GCCTGGGCTG CCTGGGACTC CTGGAAGCCT TGTGTCTTCA
    CTGGAGAGCA CCATCACCCT GAAAGGCGAC AAGGGGGATC GTGGGAAGGA TGGCTTGAAA
    GGCGAAAGAG GTCCTCCAGG CCCAAAGGGA GAACCCGGTC CACCAGGCAA AGGAGTGGAA
    ATGAAGGATC TGGAGAGGCT TTTTGAAGCA AATGGTATCA AGCTGGCACT GCTGAAGGAC
    CTCTCCAATG CACTCCTGCA GGATGGGCTG GATGGGCTGG TGTGGCAGCT GGGTGGCTCC
    AGGACAAGCA AGACCTCCAG GAGAAAGCAA GCAGTTCTTC ATGCTACTGA GCATGGCGAT
    TCACTGGTGT TTGATACTTC TCCTGGTGCC CTGGCCAGCA CTGAGGTGAC AGAAGAAGCA
    CAGAGCCTGG AGGTGGAGGC TCAGTTTAGG GTGCCGGTGT CTGGAGGACT CCTTAAGGGT
    CCATCTGCTG GTGGTTCTGA ACCTGAGTAC CAGCCCCTAG GAAACCCTCC TGAGTCCTTG
    GAGAGAACAT CTGAGGAAGG AAAGGAGGAG AGAGACCCAA GATGGACAAT CACTTCTCTC
    AGTGGTAGCT TGGCTTTGCC CCCACTGCAC CCAGAAGATG ATCTGCAAGG AAACCAGTCC
    ATGGAGCTTC TTGAGTCCCT GGAGGGGAGA GTGGAAGGAC AACCACAGCA GGACACTAGC
    CACGAAAATG AGAATTTAGA CGACGGCTCT GCGCTCCCAA GGCACGTTAG AGCCCAGAGA
    TCTGCTGAGG ACCAGAGACA CGTGGTAAAG ACCCCATTTC ACCACGACAG CTTCGGGCTG
    GCCTTTGTGC AAGAGCAGAG CCATCCGTGT GACCCAGGAG GAAACGGCGA CCTTTCCTTG
    TTGGCTCCAT CACAGGTTCA TCCCCCTGGT GCTCCAGGAG CTCGGAAATC CCCATCTGGA
    TCCACGGCAT ACCCAGGCCC TCATCACCCA AGGAGAATTA AGAAGGAGGA AGCTGGTCCA
    CCAGATCCAC CATCCCATGG CCAAAAGGGA GAAATGGGAG CTCCGGGAGT CCGTGGAGAC
    AAAGGAGAAA AGGGGCAGCC TGGGGAGATG GGGGAACCTG GAGAAAAGGG CATCAAAGGA
    GACAGAGGTG AAAATGGTCA AAAGGGAGAA CCAGGAATTG GGTTCAGAGG CCCTGTTGGT
    CAGGCTGGAC CACCTGGATT TAAAGGTGAG CCAGGGGCTC CAGGGCCTCC AGGAGCTCAG
    GGAGTCCAGG GAATTCGTGG CAATGCCGGC ACCCCCGGGT CACAAGGGGA AAGAGGGGCT
    CCAGGTCTGC CTGGGATGCC AGGACAAAAG GGAGAGCGTG GGAAGAGAGG ACGGAATGGA
    TTTGCTGGGC CCTCAGGACC ATCAGGATCC CCAGGAAAAG AGGGGATTTC TGGGACACCT
    GGACCCAAAG GCAACAAGGG AGAAATCGGC GTTGGAGCAG CTGGCCCAAG AGGTCCTCGT
    GGACTGCCTG GCCCTCGGGG TGACGAGGGC ATCGTGGGAG TCCGTGGCCC CACCGGCATG
    ACGGGTCTAA AAGGCTTCCC GGGTGTGAAG GGCGAAAGAG GTGATCGGGG ACTTCTGGGA
    CCCAAAGGAG AAAGAGGTGA GCCCATGACT ATTTTTGGAC CCCAAGGCTA TAAAGGCAGT
    AAAGGAGATC CTGGTGAACG GGGCCTGCCA GGTTTTGACG GAGACAAAGG CGAGAAAGGA
    GAGGATGGGC CTATAGGAGA AAAGGGAGTC AAAGGTGAAG CTGGCTCCAA GGGAGTGATG
    GGGCTTTTTG GAACAAGAGG ACCAGTGGGA CAGAAGGGGG AGCCAGGAGA ACCAGGCTTG
    CCGGGCTCTG CTGGTGCAGC AGGTCTTAAT GGGAAGAACG GAAACAAGGG AGCCAAAGGT
    GACAGAGGTC TTCAGGGGCA GAAGGGGAAG CCGGGAGGAA AAGGTGATCC TGGGGCCACT
    GGTGACATTG GACAGAAAGG AACAAAGGGC TTACGAGGGC TTCCAGGACG CATCGGTGCT
    CCTGGAGCTG AAGGCATGAA GGGAGAAATT GGCAGTCCTG GAAAACCAGG AATCCCAGGA
    TCAGATGGAC CACACGGACA AAAGGGTGAA AAGGGAACAT CAGGAGACGA TGGTGCCTCG
    GGGATTCCTG GGGAGAAAGG AGAAAAGGGA GCCAAAGGAG TTCCTGGCTT GGGAGGCTTC
    AAAGGACAAA TGGGATGGCC TGGGAGGACT GGAGTTGCTG GACCAGATGG CCCTCAAGGG
    CCACAGGGTG AACCAGGGCC CCAGGGTCCA CGAGGGAAAA GAGGGAGACC TCAGCTCTGC
    CTCAGGGGAC CCCCAGGCAT GGATGGGAGA AAAGGAGAAA TGGGTGAAAA TGGTCCAGCC
    GGCCAGAAAG GAGAGAAGGG AGATCCTGGT CTTTCTGAGG AAGAGGTGAA GGAGGTGGTC
    AGGAGCGAAA TGAGAGGCAG ATGCGATTGT GGAGGCGAGT TGGGAAGGAC ACTCATGGAA
    AGGGGCCTGG AGGCAACTGA AATCAACAGG CAGCTCCAAG GGTTCCAAAA GTTCCAGCTT
    TGGGAACGCA CCAGAAGGGA TGGTGAAAGC CAGGAAGAAA TGCTGAACAA AACCCAGGGG
    ATGACCATGT TGATGACAAT GGTGAAGCCC GTCATGGAGC CACCTTCACT TATTGCAGCG
    GCTCCCCGGG CGCAGCAGAT AACAGGTCCC TCTCAGCGCA AGCGGCGAGA AGCCCACAGT
    CAATTACCAG CCCCCTGCCT GCAGCCGATG GATGAAGGCT CCTGCCAGCA CTATGCCCTG
    CTCTGGTATT ACCACCTGGA GGCAAACACC TGCCGGCCCT TCATCTTTGG GGGATGCCGA
    GGGAACAGCA ACCGCTTCGA AACCAAGTGG AAGTGTGAGC AGAAGTGCAA AACCTCAGCA
    GCCAGAGGGA GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa78934
    Clone ID Related Accession (Same CDS sequence) XM_014961016.1 , XM_014961017.1 , XM_014961015.1
    Accession Version XM_014961015.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7515bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product collagen alpha-1(VII) chain-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
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    1981
    2041
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    2641
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    3181
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    3421
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    4081
    4141
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    4381
    4441
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    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
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    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTTTGTG CCCAGATCCT TGCCCTGGCA GTGGCTGTGG TGCAGCACAG AGCTGTGGCC 
    GTTCATGAAG CATGCAGGAC GGCGGAGGTG GCCGACATTC TGTTCCTCGT GGGACAGTCA
    CAGGGCACAG GAAGGGAGAG CTCCTGGCTG GTCAAGGACT TCATCAGCAG CATGGCTCGT
    TCCTTTGAGA ACGTGGTGAT GGGCAAAGGA GGCATCCGGC TGGCAGTGGC ACTCTACGGG
    GAGAAACCAA GGATGAGTAT TGAGCTTACG GATTACGTGA CCATCGAAGA AATGTTGGTG
    GCAATTCAGG ATCTCTCCTT CAAAGGTGGA AGCTTAAAAA CAGGGTCAGC CTTGGCCTTT
    GCAGCTCGTG CCATGTCTCG TCTGGACACA CTGCGAGAGG ATGCAGCAAA GGTGGTTGTT
    TTGATCACGG ATGGAAAATC CAGCAACTCA GTTGAAGATG AAGCCCGGGT CCTGCAGGAT
    TTGGGGGTGA CGGTGTTTGC CGTAGGGATT AAGGGTGCTG ACAAGAATGA ATTGAACACA
    ATAGCTTCAG AGCCCACTGC AGAGCATGTG CTTTATGTTG AAGATTTCCA CCTCCTCCAT
    AACGTGGCCC CCAAACTCTC TCGAAGGCTC TGTTTCACTG CCTCGGAGCC ACCACGACCC
    ATCAAACAGA CTGTGCAAGC TGAGAAGATC GTCGGCCCCC GGGATTTGTA TGTCAGTGAG
    CACAGCCACA GCACGCTGAG ACTCACCTGG ATGCCAGCCA CGGGGAAGGT GACGGGCTAC
    CATGTCCATC TGCATCCCTT GCTGCCTTCA GGGCAGCCCG TTTCAGAAGA CCAGCAGCAG
    ATTGTGGTGG ACGGTGACAA AAGTACCGTG CTGGTGACTG ATCTGAAACC CAACACCAAG
    TATGTCTTCA CGGTGAGGGC CATCTATGCA GATACTGTTG GGGAGTCAGC GGTTGTCAAG
    GGGAAAACAA CACCTGTGCC TCCAGTCACT AATTTCCGTG TGATAGAAGA AGGACTTTTT
    AGCTTGAAGG TGGCTTGGAC ACCTCCGCTT GGCAAGCTGG AGGGCTACAA GATCTACATC
    CCCAGAGCCA ACAGACCAGG AATGACCTAC GAGCAGGTTC TGCGGGGTGA TGTCTCATCC
    CACGTGCTGG AGAACTTGCA GGAGGACAGA GAATACAGCA TCAGCATCTA CGCCGTGTAC
    CCCCAGGGCC CCAGCCAGCC TGTGGCTGCT GTGGGGAGAA CATTGAAGCT CCTGCCCGTG
    AAAAGCATCT TGCTGCAGAA TGAGACCACC AACACGCTCC AGGCAAGGTG GACACCCATC
    CGGGGAGCAA CGGGGTACAG GCTCACCTGG ACATCAGCAG AGGGCAGCAT CCAAGATGTG
    AACCTCAGCA ACACCTACAG CTACTACATG ATCCAGGGGC TGCAGCCTGG CACGGAGTAC
    ACTGTTACCA TCAACCCCAT CTTTGGGGAC GTCGAGGGAC CAGTGGTGAG CAGCAAAGCA
    ACGACGGTGG CATCGAGCAC CGTCCAGATG CTGAAAGTGA CTGAGATATC TACCAACAGT
    GCTCTGGTCT CCTGGGACTC AGTTGCTGGG GCCACTGGGT ATCGAGTGGC TTGGGGTCCT
    ACACCAGAGT TCTTTGGCAA AGACCGTCCT CGCCAGCTGG CCCTCAACAG GTCAATCACA
    GCCTACCAGC TCCGCAACCT GGCCCATGAC ACCGAGTACG TGATTTCCCT CTACGTGCTC
    TTTGGCTCCG TGGAAGGACC AGGGATCACA ACCTCAGCCA GGACATCTCC TCTTGGCTAC
    GTCTCCAATT TCAAGGTGAT GTCCTACACC AGCACAAGCC TGTCTCTGGT GTGGAGTGCC
    ACAGCAGCTG CCACCAAATT CAAAGTCACC TGGAGCCCTG TGGGAGCAGA AAAAGAAAAA
    CAGGTTGCCA AGACTCAGTA CCTGGGCAGT AGGGTGCTGG CTTACCAGAT TGACCAGTTA
    CTGCCCAACA CCCTGTATAA AGTCAGCGTT CGGGCTGTCT TCAACAAGAC TGAGGGACCA
    GAGGTGACTC TGACTCACCG CACAGCGCCT CTTGCAGACT CCAACCCCAT CCAGGCCGTT
    GAAGATCTGA GGATTACTGA CACCGGCGTA AATAGCTTGA AGCTGTCTTG GAGGAGACTT
    CCTGGGGTAA CTCATTATAA GATATCCTGG GCGCCGTTCA ATGGTGGCTT GGAAACCTCC
    CAGGTGGTTG CAGCAGAGAC GGTCTCCTTC ACGATCTCCA ACCTGAAGGA GAGCACCACC
    TACACCATCT GCATGTCTGC AATGATCGGG GGACAGGAGG GAAGCCCCAC ACTCCTCACA
    GCCAAAACTT TGGATTTACC CAAAGTGACT GAGTTCATGG TGCAGGAGGC TGCGGAGACC
    AGCGTTTTGC TGACATGGAC AGCTGTTCCT GGGGCATCCA CTTATCTATT AACATGGCGC
    CTGTCCTCAG CTTCTGATCT TTCCCAGGAA CTGCTGTCAG CAGCTTCTAG ATCGTATCGA
    GTGACAGGAC TGAGCACCAA AGAAATGTAT GTGTTTTCCA TAAGACCAGT CTTTGGAGAT
    ACGGAGGGAC CAGAGACAAC ACTCCTTGGG CAAACAGCGG GACCTCACAG AGAGTCACCC
    ACACCCATCA CTCCAGTGAC TTCTACAAAA CTGGGTACGA TGAGATTCCC ATCCTTATCT
    CCTAGCAAGG TGGGGACCAC TCCTCTTCCA CTGGCCACCA CCACAACAGT ACCACCAACT
    CCAGCAACCC TACTGTCGAC CACTGCAGTG TCCACACTGC TGACAACTCT CCCTGGGCCA
    ATCTGTGGCA AGTTCAAAGC AGACATTGGG TTCCTGGTGG ACGAGTCCTC GAGTATTGGC
    CAGAGCAATT TCAACAAAGT GAAGGATTTC CTCTTCCGGA TCATTTCCTA TTTCCCGAAG
    ATTGGGCCTG AAGGGACACA GGTTGCTGTT GCTCAGTACA GCGAGGAGCC CAGGGCAGCC
    TTCTACTTCA ACCAGCACCG GGACCGCAAC AGTGCCCTGA AAGCCGTCAA GGGACTGAGC
    TACGCTGGGG GCAACACAAA AACAGGTCGT GGCATAACCT ACATGCTCAA GGAGTTATTT
    CAGTCCTCCA GAGGGATGAG GCCAGATTTC CCTCACGTGC TGGTGCTGGT GACCGATGGG
    CGATCCCAGG ATGATGTGTT GCTACCAGCC AGAGCAGCCC ATGAGTTGGG GATCCGCGTC
    ATTGCTGTGG GTGTCTCAGG AGCTGACCCC GTTGAGCTCA ACAGCATCTT GCTGCAGCAG
    AACCTCCAGA ATGTTTTCTA CGTCAGCACG TTTGATGACT TCCCCCAGAT CCTCCGAGAG
    CTGATAGAGG TCATCTGTTC TGACCCCGAG CCTTCAGGGC CTCAACTACC ATCTGGGGAG
    AGCTTCAGGA GTTCACCAGC TCCTCATGGA GGCTATGACC CATACAGCTT TACTACAAAG
    GGAGAGAAAG GAGAGAGGGG TCTACCTGGG AAGGATGGAA TTCCCGGGCT GCCGGGCAGA
    CCGGGGCGGA CAGGTCCTCC TGGTCCCCCT GGACTCATGG GTCTCCCTGG CATCCAAGGT
    GATACTGGCT CTCCTGGCTA TCCTGGACCA GCGGGGCCAA AAGGAGAGAG AGGCGAACCA
    GGCTATGTCC TGGGAGGAGT AGAGGTGATT CCTGGCCGAA ATGGGCAGCC TGGCCCCCCT
    GGACAGAAAG GGCAGCCGGG GGTTCCTGGG GTAGCAGGAC CACCAGGTTT ACCAGGGCCA
    CAGGGGCCAC CAGGCATCTC CATCAAGGGT GAACGTGGGG ATTCAGGTAC CAGGGGCCCT
    CGTGGGAGGA ATGGCCTCAA GGGTGACAGA GGAGGAATGG GAGAGCCGGG GAAACCTGGG
    TTGCCAGGCC CAGTAGGGCT AGATGGTGTT CCTGGATTGC CTGGACCAAG GGGAGAAAAG
    GGTGTGATGG GACCTCCAGG AGCACCAGGA CCAAAGGGTA ACAAAGGGCA AGGTGGTGTG
    AAAGGGGAAA AGGGACTCCC AGGACCTCCA GGGCAGAAGG GAGACCAGGG GAACCCAGGA
    TTTCCAGGTG CTCCAGCTAT GGGGGTTTTG GGACCTCCAG GCAAGAAAGG TGTCAGGGGT
    GATATTGGAC CAACAGGGGC CCTGGGACCA CAAGGAGACA AAGGAATACA AGGAGACAAA
    GGAGAGAAGG GAAGCCCAGG TTTCGGGATT CCTGGTCAAC CTGGGCTGAA GGGAGATCCA
    GGCGACAGGG GAAATGTAGG TTTGTCGGGC AAACCAGGTC AGAAGGGGGA TAGAGGTCTG
    AAGGGAGAGA AAGGTGAACC AGGACTACCT GGGAAACCAG GCGAGACCGG ACTAAGAGGT
    AAAGATGGAG AACCAGGGGA GAAAGGCACC TCTGGGACCA AGGGAGAAGC TGGTGTCCCT
    GGAGAACCAG GAGAGAGAGG AATCAGGGGG CCACCTGGAC TCCCAGGACG GCCTGGAGAG
    CAAGGGATAA AAGGCGATAC AGGACCACCT GGAAAGGATG GAATCACTGG AGAGAAGGGA
    GACAAGGGCG AAGCTGGTGA GCCTGGGCTG CCTGGGACTC CTGGAAGCCT TGTGTCTTCA
    CTGGAGAGCA CCATCACCCT GAAAGGCGAC AAGGGGGATC GTGGGAAGGA TGGCTTGAAA
    GGCGAAAGAG GTCCTCCAGG CCCAAAGGGA GAACCCGGTC CACCAGGCAA AGGAGTGGAA
    ATGAAGGATC TGGAGAGGCT TTTTGAAGCA AATGGTATCA AGCTGGCACT GCTGAAGGAC
    CTCTCCAATG CACTCCTGCA GGATGGGCTG GATGGGCTGG TGTGGCAGCT GGGTGGCTCC
    AGGACAAGCA AGACCTCCAG GAGAAAGCAA GCAGTTCTTC ATGCTACTGA GCATGGCGAT
    TCACTGGTGT TTGATACTTC TCCTGGTGCC CTGGCCAGCA CTGAGGTGAC AGAAGAAGCA
    CAGAGCCTGG AGGTGGAGGC TCAGTTTAGG GTGCCGGTGT CTGGAGGACT CCTTAAGGGT
    CCATCTGCTG GTGGTTCTGA ACCTGAGTAC CAGCCCCTAG GAAACCCTCC TGAGTCCTTG
    GAGAGAACAT CTGAGGAAGG AAAGGAGGAG AGAGACCCAA GATGGACAAT CACTTCTCTC
    AGTGGTAGCT TGGCTTTGCC CCCACTGCAC CCAGAAGATG ATCTGCAAGG AAACCAGTCC
    ATGGAGCTTC TTGAGTCCCT GGAGGGGAGA GTGGAAGGAC AACCACAGCA GGACACTAGC
    CACGAAAATG AGAATTTAGA CGACGGCTCT GCGCTCCCAA GGCACGTTAG AGCCCAGAGA
    TCTGCTGAGG ACCAGAGACA CGTGGTAAAG ACCCCATTTC ACCACGACAG CTTCGGGCTG
    GCCTTTGTGC AAGAGCAGAG CCATCCGTGT GACCCAGGAG GAAACGGCGA CCTTTCCTTG
    TTGGCTCCAT CACAGGTTCA TCCCCCTGGT GCTCCAGGAG CTCGGAAATC CCCATCTGGA
    TCCACGGCAT ACCCAGGCCC TCATCACCCA AGGAGAATTA AGAAGGAGGA AGCTGGTCCA
    CCAGATCCAC CATCCCATGG CCAAAAGGGA GAAATGGGAG CTCCGGGAGT CCGTGGAGAC
    AAAGGAGAAA AGGGGCAGCC TGGGGAGATG GGGGAACCTG GAGAAAAGGG CATCAAAGGA
    GACAGAGGTG AAAATGGTCA AAAGGGAGAA CCAGGAATTG GGTTCAGAGG CCCTGTTGGT
    CAGGCTGGAC CACCTGGATT TAAAGGTGAG CCAGGGGCTC CAGGGCCTCC AGGAGCTCAG
    GGAGTCCAGG GAATTCGTGG CAATGCCGGC ACCCCCGGGT CACAAGGGGA AAGAGGGGCT
    CCAGGTCTGC CTGGGATGCC AGGACAAAAG GGAGAGCGTG GGAAGAGAGG ACGGAATGGA
    TTTGCTGGGC CCTCAGGACC ATCAGGATCC CCAGGAAAAG AGGGGATTTC TGGGACACCT
    GGACCCAAAG GCAACAAGGG AGAAATCGGC GTTGGAGCAG CTGGCCCAAG AGGTCCTCGT
    GGACTGCCTG GCCCTCGGGG TGACGAGGGC ATCGTGGGAG TCCGTGGCCC CACCGGCATG
    ACGGGTCTAA AAGGCTTCCC GGGTGTGAAG GGCGAAAGAG GTGATCGGGG ACTTCTGGGA
    CCCAAAGGAG AAAGAGGTGA GCCCATGACT ATTTTTGGAC CCCAAGGCTA TAAAGGCAGT
    AAAGGAGATC CTGGTGAACG GGGCCTGCCA GGTTTTGACG GAGACAAAGG CGAGAAAGGA
    GAGGATGGGC CTATAGGAGA AAAGGGAGTC AAAGGTGAAG CTGGCTCCAA GGGAGTGATG
    GGGCTTTTTG GAACAAGAGG ACCAGTGGGA CAGAAGGGGG AGCCAGGAGA ACCAGGCTTG
    CCGGGCTCTG CTGGTGCAGC AGGTCTTAAT GGGAAGAACG GAAACAAGGG AGCCAAAGGT
    GACAGAGGTC TTCAGGGGCA GAAGGGGAAG CCGGGAGGAA AAGGTGATCC TGGGGCCACT
    GGTGACATTG GACAGAAAGG AACAAAGGGC TTACGAGGGC TTCCAGGACG CATCGGTGCT
    CCTGGAGCTG AAGGCATGAA GGGAGAAATT GGCAGTCCTG GAAAACCAGG AATCCCAGGA
    TCAGATGGAC CACACGGACA AAAGGGTGAA AAGGGAACAT CAGGAGACGA TGGTGCCTCG
    GGGATTCCTG GGGAGAAAGG AGAAAAGGGA GCCAAAGGAG TTCCTGGCTT GGGAGGCTTC
    AAAGGACAAA TGGGATGGCC TGGGAGGACT GGAGTTGCTG GACCAGATGG CCCTCAAGGG
    CCACAGGGTG AACCAGGGCC CCAGGGTCCA CGAGGGAAAA GAGGGAGACC TCAGCTCTGC
    CTCAGGGGAC CCCCAGGCAT GGATGGGAGA AAAGGAGAAA TGGGTGAAAA TGGTCCAGCC
    GGCCAGAAAG GAGAGAAGGG AGATCCTGGT CTTTCTGAGG AAGAGGTGAA GGAGGTGGTC
    AGGAGCGAAA TGAGAGGCAG ATGCGATTGT GGAGGCGAGT TGGGAAGGAC ACTCATGGAA
    AGGGGCCTGG AGGCAACTGA AATCAACAGG CAGCTCCAAG GGTTCCAAAA GTTCCAGCTT
    TGGGAACGCA CCAGAAGGGA TGGTGAAAGC CAGGAAGAAA TGCTGAACAA AACCCAGGGG
    ATGACCATGT TGATGACAAT GGTGAAGCCC GTCATGGAGC CACCTTCACT TATTGCAGCG
    GCTCCCCGGG CGCAGCAGAT AACAGGTCCC TCTCAGCGCA AGCGGCGAGA AGCCCACAGT
    CAATTACCAG CCCCCTGCCT GCAGCCGATG GATGAAGGCT CCTGCCAGCA CTATGCCCTG
    CTCTGGTATT ACCACCTGGA GGCAAACACC TGCCGGCCCT TCATCTTTGG GGGATGCCGA
    GGGAACAGCA ACCGCTTCGA AACCAAGTGG AAGTGTGAGC AGAAGTGCAA AACCTCAGCA
    GCCAGAGGGA GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014816501.1
    CDS249..7763
    Translation

    Target ORF information:

    RefSeq Version XM_014961015.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax collagen alpha-1(VII) chain-like (LOC106899270), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014961015.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGTTTTGTG CCCAGATCCT TGCCCTGGCA GTGGCTGTGG TGCAGCACAG AGCTGTGGCC 
    GTTCATGAAG CATGCAGGAC GGCGGAGGTG GCCGACATTC TGTTCCTCGT GGGACAGTCA
    CAGGGCACAG GAAGGGAGAG CTCCTGGCTG GTCAAGGACT TCATCAGCAG CATGGCTCGT
    TCCTTTGAGA ACGTGGTGAT GGGCAAAGGA GGCATCCGGC TGGCAGTGGC ACTCTACGGG
    GAGAAACCAA GGATGAGTAT TGAGCTTACG GATTACGTGA CCATCGAAGA AATGTTGGTG
    GCAATTCAGG ATCTCTCCTT CAAAGGTGGA AGCTTAAAAA CAGGGTCAGC CTTGGCCTTT
    GCAGCTCGTG CCATGTCTCG TCTGGACACA CTGCGAGAGG ATGCAGCAAA GGTGGTTGTT
    TTGATCACGG ATGGAAAATC CAGCAACTCA GTTGAAGATG AAGCCCGGGT CCTGCAGGAT
    TTGGGGGTGA CGGTGTTTGC CGTAGGGATT AAGGGTGCTG ACAAGAATGA ATTGAACACA
    ATAGCTTCAG AGCCCACTGC AGAGCATGTG CTTTATGTTG AAGATTTCCA CCTCCTCCAT
    AACGTGGCCC CCAAACTCTC TCGAAGGCTC TGTTTCACTG CCTCGGAGCC ACCACGACCC
    ATCAAACAGA CTGTGCAAGC TGAGAAGATC GTCGGCCCCC GGGATTTGTA TGTCAGTGAG
    CACAGCCACA GCACGCTGAG ACTCACCTGG ATGCCAGCCA CGGGGAAGGT GACGGGCTAC
    CATGTCCATC TGCATCCCTT GCTGCCTTCA GGGCAGCCCG TTTCAGAAGA CCAGCAGCAG
    ATTGTGGTGG ACGGTGACAA AAGTACCGTG CTGGTGACTG ATCTGAAACC CAACACCAAG
    TATGTCTTCA CGGTGAGGGC CATCTATGCA GATACTGTTG GGGAGTCAGC GGTTGTCAAG
    GGGAAAACAA CACCTGTGCC TCCAGTCACT AATTTCCGTG TGATAGAAGA AGGACTTTTT
    AGCTTGAAGG TGGCTTGGAC ACCTCCGCTT GGCAAGCTGG AGGGCTACAA GATCTACATC
    CCCAGAGCCA ACAGACCAGG AATGACCTAC GAGCAGGTTC TGCGGGGTGA TGTCTCATCC
    CACGTGCTGG AGAACTTGCA GGAGGACAGA GAATACAGCA TCAGCATCTA CGCCGTGTAC
    CCCCAGGGCC CCAGCCAGCC TGTGGCTGCT GTGGGGAGAA CATTGAAGCT CCTGCCCGTG
    AAAAGCATCT TGCTGCAGAA TGAGACCACC AACACGCTCC AGGCAAGGTG GACACCCATC
    CGGGGAGCAA CGGGGTACAG GCTCACCTGG ACATCAGCAG AGGGCAGCAT CCAAGATGTG
    AACCTCAGCA ACACCTACAG CTACTACATG ATCCAGGGGC TGCAGCCTGG CACGGAGTAC
    ACTGTTACCA TCAACCCCAT CTTTGGGGAC GTCGAGGGAC CAGTGGTGAG CAGCAAAGCA
    ACGACGGTGG CATCGAGCAC CGTCCAGATG CTGAAAGTGA CTGAGATATC TACCAACAGT
    GCTCTGGTCT CCTGGGACTC AGTTGCTGGG GCCACTGGGT ATCGAGTGGC TTGGGGTCCT
    ACACCAGAGT TCTTTGGCAA AGACCGTCCT CGCCAGCTGG CCCTCAACAG GTCAATCACA
    GCCTACCAGC TCCGCAACCT GGCCCATGAC ACCGAGTACG TGATTTCCCT CTACGTGCTC
    TTTGGCTCCG TGGAAGGACC AGGGATCACA ACCTCAGCCA GGACATCTCC TCTTGGCTAC
    GTCTCCAATT TCAAGGTGAT GTCCTACACC AGCACAAGCC TGTCTCTGGT GTGGAGTGCC
    ACAGCAGCTG CCACCAAATT CAAAGTCACC TGGAGCCCTG TGGGAGCAGA AAAAGAAAAA
    CAGGTTGCCA AGACTCAGTA CCTGGGCAGT AGGGTGCTGG CTTACCAGAT TGACCAGTTA
    CTGCCCAACA CCCTGTATAA AGTCAGCGTT CGGGCTGTCT TCAACAAGAC TGAGGGACCA
    GAGGTGACTC TGACTCACCG CACAGCGCCT CTTGCAGACT CCAACCCCAT CCAGGCCGTT
    GAAGATCTGA GGATTACTGA CACCGGCGTA AATAGCTTGA AGCTGTCTTG GAGGAGACTT
    CCTGGGGTAA CTCATTATAA GATATCCTGG GCGCCGTTCA ATGGTGGCTT GGAAACCTCC
    CAGGTGGTTG CAGCAGAGAC GGTCTCCTTC ACGATCTCCA ACCTGAAGGA GAGCACCACC
    TACACCATCT GCATGTCTGC AATGATCGGG GGACAGGAGG GAAGCCCCAC ACTCCTCACA
    GCCAAAACTT TGGATTTACC CAAAGTGACT GAGTTCATGG TGCAGGAGGC TGCGGAGACC
    AGCGTTTTGC TGACATGGAC AGCTGTTCCT GGGGCATCCA CTTATCTATT AACATGGCGC
    CTGTCCTCAG CTTCTGATCT TTCCCAGGAA CTGCTGTCAG CAGCTTCTAG ATCGTATCGA
    GTGACAGGAC TGAGCACCAA AGAAATGTAT GTGTTTTCCA TAAGACCAGT CTTTGGAGAT
    ACGGAGGGAC CAGAGACAAC ACTCCTTGGG CAAACAGCGG GACCTCACAG AGAGTCACCC
    ACACCCATCA CTCCAGTGAC TTCTACAAAA CTGGGTACGA TGAGATTCCC ATCCTTATCT
    CCTAGCAAGG TGGGGACCAC TCCTCTTCCA CTGGCCACCA CCACAACAGT ACCACCAACT
    CCAGCAACCC TACTGTCGAC CACTGCAGTG TCCACACTGC TGACAACTCT CCCTGGGCCA
    ATCTGTGGCA AGTTCAAAGC AGACATTGGG TTCCTGGTGG ACGAGTCCTC GAGTATTGGC
    CAGAGCAATT TCAACAAAGT GAAGGATTTC CTCTTCCGGA TCATTTCCTA TTTCCCGAAG
    ATTGGGCCTG AAGGGACACA GGTTGCTGTT GCTCAGTACA GCGAGGAGCC CAGGGCAGCC
    TTCTACTTCA ACCAGCACCG GGACCGCAAC AGTGCCCTGA AAGCCGTCAA GGGACTGAGC
    TACGCTGGGG GCAACACAAA AACAGGTCGT GGCATAACCT ACATGCTCAA GGAGTTATTT
    CAGTCCTCCA GAGGGATGAG GCCAGATTTC CCTCACGTGC TGGTGCTGGT GACCGATGGG
    CGATCCCAGG ATGATGTGTT GCTACCAGCC AGAGCAGCCC ATGAGTTGGG GATCCGCGTC
    ATTGCTGTGG GTGTCTCAGG AGCTGACCCC GTTGAGCTCA ACAGCATCTT GCTGCAGCAG
    AACCTCCAGA ATGTTTTCTA CGTCAGCACG TTTGATGACT TCCCCCAGAT CCTCCGAGAG
    CTGATAGAGG TCATCTGTTC TGACCCCGAG CCTTCAGGGC CTCAACTACC ATCTGGGGAG
    AGCTTCAGGA GTTCACCAGC TCCTCATGGA GGCTATGACC CATACAGCTT TACTACAAAG
    GGAGAGAAAG GAGAGAGGGG TCTACCTGGG AAGGATGGAA TTCCCGGGCT GCCGGGCAGA
    CCGGGGCGGA CAGGTCCTCC TGGTCCCCCT GGACTCATGG GTCTCCCTGG CATCCAAGGT
    GATACTGGCT CTCCTGGCTA TCCTGGACCA GCGGGGCCAA AAGGAGAGAG AGGCGAACCA
    GGCTATGTCC TGGGAGGAGT AGAGGTGATT CCTGGCCGAA ATGGGCAGCC TGGCCCCCCT
    GGACAGAAAG GGCAGCCGGG GGTTCCTGGG GTAGCAGGAC CACCAGGTTT ACCAGGGCCA
    CAGGGGCCAC CAGGCATCTC CATCAAGGGT GAACGTGGGG ATTCAGGTAC CAGGGGCCCT
    CGTGGGAGGA ATGGCCTCAA GGGTGACAGA GGAGGAATGG GAGAGCCGGG GAAACCTGGG
    TTGCCAGGCC CAGTAGGGCT AGATGGTGTT CCTGGATTGC CTGGACCAAG GGGAGAAAAG
    GGTGTGATGG GACCTCCAGG AGCACCAGGA CCAAAGGGTA ACAAAGGGCA AGGTGGTGTG
    AAAGGGGAAA AGGGACTCCC AGGACCTCCA GGGCAGAAGG GAGACCAGGG GAACCCAGGA
    TTTCCAGGTG CTCCAGCTAT GGGGGTTTTG GGACCTCCAG GCAAGAAAGG TGTCAGGGGT
    GATATTGGAC CAACAGGGGC CCTGGGACCA CAAGGAGACA AAGGAATACA AGGAGACAAA
    GGAGAGAAGG GAAGCCCAGG TTTCGGGATT CCTGGTCAAC CTGGGCTGAA GGGAGATCCA
    GGCGACAGGG GAAATGTAGG TTTGTCGGGC AAACCAGGTC AGAAGGGGGA TAGAGGTCTG
    AAGGGAGAGA AAGGTGAACC AGGACTACCT GGGAAACCAG GCGAGACCGG ACTAAGAGGT
    AAAGATGGAG AACCAGGGGA GAAAGGCACC TCTGGGACCA AGGGAGAAGC TGGTGTCCCT
    GGAGAACCAG GAGAGAGAGG AATCAGGGGG CCACCTGGAC TCCCAGGACG GCCTGGAGAG
    CAAGGGATAA AAGGCGATAC AGGACCACCT GGAAAGGATG GAATCACTGG AGAGAAGGGA
    GACAAGGGCG AAGCTGGTGA GCCTGGGCTG CCTGGGACTC CTGGAAGCCT TGTGTCTTCA
    CTGGAGAGCA CCATCACCCT GAAAGGCGAC AAGGGGGATC GTGGGAAGGA TGGCTTGAAA
    GGCGAAAGAG GTCCTCCAGG CCCAAAGGGA GAACCCGGTC CACCAGGCAA AGGAGTGGAA
    ATGAAGGATC TGGAGAGGCT TTTTGAAGCA AATGGTATCA AGCTGGCACT GCTGAAGGAC
    CTCTCCAATG CACTCCTGCA GGATGGGCTG GATGGGCTGG TGTGGCAGCT GGGTGGCTCC
    AGGACAAGCA AGACCTCCAG GAGAAAGCAA GCAGTTCTTC ATGCTACTGA GCATGGCGAT
    TCACTGGTGT TTGATACTTC TCCTGGTGCC CTGGCCAGCA CTGAGGTGAC AGAAGAAGCA
    CAGAGCCTGG AGGTGGAGGC TCAGTTTAGG GTGCCGGTGT CTGGAGGACT CCTTAAGGGT
    CCATCTGCTG GTGGTTCTGA ACCTGAGTAC CAGCCCCTAG GAAACCCTCC TGAGTCCTTG
    GAGAGAACAT CTGAGGAAGG AAAGGAGGAG AGAGACCCAA GATGGACAAT CACTTCTCTC
    AGTGGTAGCT TGGCTTTGCC CCCACTGCAC CCAGAAGATG ATCTGCAAGG AAACCAGTCC
    ATGGAGCTTC TTGAGTCCCT GGAGGGGAGA GTGGAAGGAC AACCACAGCA GGACACTAGC
    CACGAAAATG AGAATTTAGA CGACGGCTCT GCGCTCCCAA GGCACGTTAG AGCCCAGAGA
    TCTGCTGAGG ACCAGAGACA CGTGGTAAAG ACCCCATTTC ACCACGACAG CTTCGGGCTG
    GCCTTTGTGC AAGAGCAGAG CCATCCGTGT GACCCAGGAG GAAACGGCGA CCTTTCCTTG
    TTGGCTCCAT CACAGGTTCA TCCCCCTGGT GCTCCAGGAG CTCGGAAATC CCCATCTGGA
    TCCACGGCAT ACCCAGGCCC TCATCACCCA AGGAGAATTA AGAAGGAGGA AGCTGGTCCA
    CCAGATCCAC CATCCCATGG CCAAAAGGGA GAAATGGGAG CTCCGGGAGT CCGTGGAGAC
    AAAGGAGAAA AGGGGCAGCC TGGGGAGATG GGGGAACCTG GAGAAAAGGG CATCAAAGGA
    GACAGAGGTG AAAATGGTCA AAAGGGAGAA CCAGGAATTG GGTTCAGAGG CCCTGTTGGT
    CAGGCTGGAC CACCTGGATT TAAAGGTGAG CCAGGGGCTC CAGGGCCTCC AGGAGCTCAG
    GGAGTCCAGG GAATTCGTGG CAATGCCGGC ACCCCCGGGT CACAAGGGGA AAGAGGGGCT
    CCAGGTCTGC CTGGGATGCC AGGACAAAAG GGAGAGCGTG GGAAGAGAGG ACGGAATGGA
    TTTGCTGGGC CCTCAGGACC ATCAGGATCC CCAGGAAAAG AGGGGATTTC TGGGACACCT
    GGACCCAAAG GCAACAAGGG AGAAATCGGC GTTGGAGCAG CTGGCCCAAG AGGTCCTCGT
    GGACTGCCTG GCCCTCGGGG TGACGAGGGC ATCGTGGGAG TCCGTGGCCC CACCGGCATG
    ACGGGTCTAA AAGGCTTCCC GGGTGTGAAG GGCGAAAGAG GTGATCGGGG ACTTCTGGGA
    CCCAAAGGAG AAAGAGGTGA GCCCATGACT ATTTTTGGAC CCCAAGGCTA TAAAGGCAGT
    AAAGGAGATC CTGGTGAACG GGGCCTGCCA GGTTTTGACG GAGACAAAGG CGAGAAAGGA
    GAGGATGGGC CTATAGGAGA AAAGGGAGTC AAAGGTGAAG CTGGCTCCAA GGGAGTGATG
    GGGCTTTTTG GAACAAGAGG ACCAGTGGGA CAGAAGGGGG AGCCAGGAGA ACCAGGCTTG
    CCGGGCTCTG CTGGTGCAGC AGGTCTTAAT GGGAAGAACG GAAACAAGGG AGCCAAAGGT
    GACAGAGGTC TTCAGGGGCA GAAGGGGAAG CCGGGAGGAA AAGGTGATCC TGGGGCCACT
    GGTGACATTG GACAGAAAGG AACAAAGGGC TTACGAGGGC TTCCAGGACG CATCGGTGCT
    CCTGGAGCTG AAGGCATGAA GGGAGAAATT GGCAGTCCTG GAAAACCAGG AATCCCAGGA
    TCAGATGGAC CACACGGACA AAAGGGTGAA AAGGGAACAT CAGGAGACGA TGGTGCCTCG
    GGGATTCCTG GGGAGAAAGG AGAAAAGGGA GCCAAAGGAG TTCCTGGCTT GGGAGGCTTC
    AAAGGACAAA TGGGATGGCC TGGGAGGACT GGAGTTGCTG GACCAGATGG CCCTCAAGGG
    CCACAGGGTG AACCAGGGCC CCAGGGTCCA CGAGGGAAAA GAGGGAGACC TCAGCTCTGC
    CTCAGGGGAC CCCCAGGCAT GGATGGGAGA AAAGGAGAAA TGGGTGAAAA TGGTCCAGCC
    GGCCAGAAAG GAGAGAAGGG AGATCCTGGT CTTTCTGAGG AAGAGGTGAA GGAGGTGGTC
    AGGAGCGAAA TGAGAGGCAG ATGCGATTGT GGAGGCGAGT TGGGAAGGAC ACTCATGGAA
    AGGGGCCTGG AGGCAACTGA AATCAACAGG CAGCTCCAAG GGTTCCAAAA GTTCCAGCTT
    TGGGAACGCA CCAGAAGGGA TGGTGAAAGC CAGGAAGAAA TGCTGAACAA AACCCAGGGG
    ATGACCATGT TGATGACAAT GGTGAAGCCC GTCATGGAGC CACCTTCACT TATTGCAGCG
    GCTCCCCGGG CGCAGCAGAT AACAGGTCCC TCTCAGCGCA AGCGGCGAGA AGCCCACAGT
    CAATTACCAG CCCCCTGCCT GCAGCCGATG GATGAAGGCT CCTGCCAGCA CTATGCCCTG
    CTCTGGTATT ACCACCTGGA GGCAAACACC TGCCGGCCCT TCATCTTTGG GGGATGCCGA
    GGGAACAGCA ACCGCTTCGA AACCAAGTGG AAGTGTGAGC AGAAGTGCAA AACCTCAGCA
    GCCAGAGGGA GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa78935
    Clone ID Related Accession (Same CDS sequence) XM_014961018.1
    Accession Version XM_014961018.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7479bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product collagen alpha-1(VII) chain-like isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTTTGTG CCCAGATCCT TGCCCTGGCA GTGGCTGTGG TGCAGCACAG AGCTGTGGCC 
    GTTCATGAAG CATGCAGGAC GGCGGAGGTG GCCGACATTC TGTTCCTCGT GGGACAGTCA
    CAGGGCACAG GAAGGGAGAG CTCCTGGCTG GTCAAGGACT TCATCAGCAG CATGGCTCGT
    TCCTTTGAGA ACGTGGTGAT GGGCAAAGGA GGCATCCGGC TGGCAGTGGC ACTCTACGGG
    GAGAAACCAA GGATGAGTAT TGAGCTTACG GATTACGTGA CCATCGAAGA AATGTTGGTG
    GCAATTCAGG ATCTCTCCTT CAAAGGTGGA AGCTTAAAAA CAGGGTCAGC CTTGGCCTTT
    GCAGCTCGTG CCATGTCTCG TCTGGACACA CTGCGAGAGG ATGCAGCAAA GGTGGTTGTT
    TTGATCACGG ATGGAAAATC CAGCAACTCA GTTGAAGATG AAGCCCGGGT CCTGCAGGAT
    TTGGGGGTGA CGGTGTTTGC CGTAGGGATT AAGGGTGCTG ACAAGAATGA ATTGAACACA
    ATAGCTTCAG AGCCCACTGC AGAGCATGTG CTTTATGTTG AAGATTTCCA CCTCCTCCAT
    AACGTGGCCC CCAAACTCTC TCGAAGGCTC TGTTTCACTG CCTCGGAGCC ACCACGACCC
    ATCAAACAGA CTGTGCAAGC TGAGAAGATC GTCGGCCCCC GGGATTTGTA TGTCAGTGAG
    CACAGCCACA GCACGCTGAG ACTCACCTGG ATGCCAGCCA CGGGGAAGGT GACGGGCTAC
    CATGTCCATC TGCATCCCTT GCTGCCTTCA GGGCAGCCCG TTTCAGAAGA CCAGCAGCAG
    ATTGTGGTGG ACGGTGACAA AAGTACCGTG CTGGTGACTG ATCTGAAACC CAACACCAAG
    TATGTCTTCA CGGTGAGGGC CATCTATGCA GATACTGTTG GGGAGTCAGC GGTTGTCAAG
    GGGAAAACAA CACCTGTGCC TCCAGTCACT AATTTCCGTG TGATAGAAGA AGGACTTTTT
    AGCTTGAAGG TGGCTTGGAC ACCTCCGCTT GGCAAGCTGG AGGGCTACAA GATCTACATC
    CCCAGAGCCA ACAGACCAGG AATGACCTAC GAGCAGGTTC TGCGGGGTGA TGTCTCATCC
    CACGTGCTGG AGAACTTGCA GGAGGACAGA GAATACAGCA TCAGCATCTA CGCCGTGTAC
    CCCCAGGGCC CCAGCCAGCC TGTGGCTGCT GTGGGGAGAA CATTGAAGCT CCTGCCCGTG
    AAAAGCATCT TGCTGCAGAA TGAGACCACC AACACGCTCC AGGCAAGGTG GACACCCATC
    CGGGGAGCAA CGGGGTACAG GCTCACCTGG ACATCAGCAG AGGGCAGCAT CCAAGATGTG
    AACCTCAGCA ACACCTACAG CTACTACATG ATCCAGGGGC TGCAGCCTGG CACGGAGTAC
    ACTGTTACCA TCAACCCCAT CTTTGGGGAC GTCGAGGGAC CAGTGGTGAG CAGCAAAGCA
    ACGACGGTGG CATCGAGCAC CGTCCAGATG CTGAAAGTGA CTGAGATATC TACCAACAGT
    GCTCTGGTCT CCTGGGACTC AGTTGCTGGG GCCACTGGGT ATCGAGTGGC TTGGGGTCCT
    ACACCAGAGT TCTTTGGCAA AGACCGTCCT CGCCAGCTGG CCCTCAACAG GTCAATCACA
    GCCTACCAGC TCCGCAACCT GGCCCATGAC ACCGAGTACG TGATTTCCCT CTACGTGCTC
    TTTGGCTCCG TGGAAGGACC AGGGATCACA ACCTCAGCCA GGACATCTCC TCTTGGCTAC
    GTCTCCAATT TCAAGGTGAT GTCCTACACC AGCACAAGCC TGTCTCTGGT GTGGAGTGCC
    ACAGCAGCTG CCACCAAATT CAAAGTCACC TGGAGCCCTG TGGGAGCAGA AAAAGAAAAA
    CAGGTTGCCA AGACTCAGTA CCTGGGCAGT AGGGTGCTGG CTTACCAGAT TGACCAGTTA
    CTGCCCAACA CCCTGTATAA AGTCAGCGTT CGGGCTGTCT TCAACAAGAC TGAGGGACCA
    GAGGTGACTC TGACTCACCG CACAGCGCCT CTTGCAGACT CCAACCCCAT CCAGGCCGTT
    GAAGATCTGA GGATTACTGA CACCGGCGTA AATAGCTTGA AGCTGTCTTG GAGGAGACTT
    CCTGGGGTAA CTCATTATAA GATATCCTGG GCGCCGTTCA ATGGTGGCTT GGAAACCTCC
    CAGGTGGTTG CAGCAGAGAC GGTCTCCTTC ACGATCTCCA ACCTGAAGGA GAGCACCACC
    TACACCATCT GCATGTCTGC AATGATCGGG GGACAGGAGG GAAGCCCCAC ACTCCTCACA
    GCCAAAACTT TGGATTTACC CAAAGTGACT GAGTTCATGG TGCAGGAGGC TGCGGAGACC
    AGCGTTTTGC TGACATGGAC AGCTGTTCCT GGGGCATCCA CTTATCTATT AACATGGCGC
    CTGTCCTCAG CTTCTGATCT TTCCCAGGAA CTGCTGTCAG CAGCTTCTAG ATCGTATCGA
    GTGACAGGAC TGAGCACCAA AGAAATGTAT GTGTTTTCCA TAAGACCAGT CTTTGGAGAT
    ACGGAGGGAC CAGAGACAAC ACTCCTTGGG CAAACAGCGG GACCTCACAG AGAGTCACCC
    ACACCCATCA CTCCAGTGAC TTCTACAAAA CTGGGTACGA TGAGATTCCC ATCCTTATCT
    CCTAGCAAGG TGGGGACCAC TCCTCTTCCA CTGGCCACCA CCACAACAGT ACCACCAACT
    CCAGCAACCC TACTGTCGAC CACTGCAGTG TCCACACTGC TGACAACTCT CCCTGGGCCA
    ATCTGTGGCA AGTTCAAAGC AGACATTGGG TTCCTGGTGG ACGAGTCCTC GAGTATTGGC
    CAGAGCAATT TCAACAAAGT GAAGGATTTC CTCTTCCGGA TCATTTCCTA TTTCCCGAAG
    ATTGGGCCTG AAGGGACACA GGTTGCTGTT GCTCAGTACA GCGAGGAGCC CAGGGCAGCC
    TTCTACTTCA ACCAGCACCG GGACCGCAAC AGTGCCCTGA AAGCCGTCAA GGGACTGAGC
    TACGCTGGGG GCAACACAAA AACAGGTCGT GGCATAACCT ACATGCTCAA GGAGTTATTT
    CAGTCCTCCA GAGGGATGAG GCCAGATTTC CCTCACGTGC TGGTGCTGGT GACCGATGGG
    CGATCCCAGG ATGATGTGTT GCTACCAGCC AGAGCAGCCC ATGAGTTGGG GATCCGCGTC
    ATTGCTGTGG GTGTCTCAGG AGCTGACCCC GTTGAGCTCA ACAGCATCTT GCTGCAGCAG
    AACCTCCAGA ATGTTTTCTA CGTCAGCACG TTTGATGACT TCCCCCAGAT CCTCCGAGAG
    CTGATAGAGG TCATCTGTTC TGACCCCGAG CCTTCAGGGC CTCAACTACC ATCTGGGGAG
    AGCTTCAGGA GTTCACCAGC TCCTCATGGA GGCTATGACC CATACAGCTT TACTACAAAG
    GGAGAGAAAG GAGAGAGGGG TCTACCTGGG AAGGATGGAA TTCCCGGGCT GCCGGGCAGA
    CCGGGGCGGA CAGGTCCTCC TGGTCCCCCT GGACTCATGG GTCTCCCTGG CATCCAAGGT
    GATACTGGCT CTCCTGGCTA TCCTGGACCA GCGGGGCCAA AAGGAGAGAG AGGCGAACCA
    GGCTATGTCC TGGGAGGAGT AGAGGTGATT CCTGGCCGAA ATGGGCAGCC TGGCCCCCCT
    GGACAGAAAG GGCAGCCGGG GGTTCCTGGG GTAGCAGGAC CACCAGGTTT ACCAGGGCCA
    CAGGGGCCAC CAGGCATCTC CATCAAGGGT GAACGTGGGG ATTCAGGTAC CAGGGGCCCT
    CGTGGGAGGA ATGGCCTCAA GGGTGACAGA GGAGGAATGG GAGAGCCGGG GAAACCTGGG
    TTGCCAGGCC CAGTAGGGCT AGATGGTGTT CCTGGATTGC CTGGACCAAG GGGAGAAAAG
    GGTGTGATGG GACCTCCAGG AGCACCAGGA CCAAAGGGTA ACAAAGGGCA AGGTGGTGTG
    AAAGGGGAAA AGGGACTCCC AGGACCTCCA GGGCAGAAGG GAGACCAGGG GAACCCAGGA
    TTTCCAGGTG CTCCAGCTAT GGGGGTTTTG GGACCTCCAG GCAAGAAAGG TGTCAGGGGT
    GATATTGGAC CAACAGGGGC CCTGGGACCA CAAGGAGACA AAGGAATACA AGGAGACAAA
    GGAGAGAAGG GAAGCCCAGG TTTCGGGATT CCTGGTCAAC CTGGGCTGAA GGGAGATCCA
    GGCGACAGGG GAAATGTAGG TTTGTCGGGC AAACCAGGTC AGAAGGGGGA TAGAGGTCTG
    AAGGGAGAGA AAGGTGAACC AGGACTACCT GGGAAACCAG GCGAGACCGG ACTAAGAGGT
    AAAGATGGAG AAGCTGGTGT CCCTGGAGAA CCAGGAGAGA GAGGAATCAG GGGGCCACCT
    GGACTCCCAG GACGGCCTGG AGAGCAAGGG ATAAAAGGCG ATACAGGACC ACCTGGAAAG
    GATGGAATCA CTGGAGAGAA GGGAGACAAG GGCGAAGCTG GTGAGCCTGG GCTGCCTGGG
    ACTCCTGGAA GCCTTGTGTC TTCACTGGAG AGCACCATCA CCCTGAAAGG CGACAAGGGG
    GATCGTGGGA AGGATGGCTT GAAAGGCGAA AGAGGTCCTC CAGGCCCAAA GGGAGAACCC
    GGTCCACCAG GCAAAGGAGT GGAAATGAAG GATCTGGAGA GGCTTTTTGA AGCAAATGGT
    ATCAAGCTGG CACTGCTGAA GGACCTCTCC AATGCACTCC TGCAGGATGG GCTGGATGGG
    CTGGTGTGGC AGCTGGGTGG CTCCAGGACA AGCAAGACCT CCAGGAGAAA GCAAGCAGTT
    CTTCATGCTA CTGAGCATGG CGATTCACTG GTGTTTGATA CTTCTCCTGG TGCCCTGGCC
    AGCACTGAGG TGACAGAAGA AGCACAGAGC CTGGAGGTGG AGGCTCAGTT TAGGGTGCCG
    GTGTCTGGAG GACTCCTTAA GGGTCCATCT GCTGGTGGTT CTGAACCTGA GTACCAGCCC
    CTAGGAAACC CTCCTGAGTC CTTGGAGAGA ACATCTGAGG AAGGAAAGGA GGAGAGAGAC
    CCAAGATGGA CAATCACTTC TCTCAGTGGT AGCTTGGCTT TGCCCCCACT GCACCCAGAA
    GATGATCTGC AAGGAAACCA GTCCATGGAG CTTCTTGAGT CCCTGGAGGG GAGAGTGGAA
    GGACAACCAC AGCAGGACAC TAGCCACGAA AATGAGAATT TAGACGACGG CTCTGCGCTC
    CCAAGGCACG TTAGAGCCCA GAGATCTGCT GAGGACCAGA GACACGTGGT AAAGACCCCA
    TTTCACCACG ACAGCTTCGG GCTGGCCTTT GTGCAAGAGC AGAGCCATCC GTGTGACCCA
    GGAGGAAACG GCGACCTTTC CTTGTTGGCT CCATCACAGG TTCATCCCCC TGGTGCTCCA
    GGAGCTCGGA AATCCCCATC TGGATCCACG GCATACCCAG GCCCTCATCA CCCAAGGAGA
    ATTAAGAAGG AGGAAGCTGG TCCACCAGAT CCACCATCCC ATGGCCAAAA GGGAGAAATG
    GGAGCTCCGG GAGTCCGTGG AGACAAAGGA GAAAAGGGGC AGCCTGGGGA GATGGGGGAA
    CCTGGAGAAA AGGGCATCAA AGGAGACAGA GGTGAAAATG GTCAAAAGGG AGAACCAGGA
    ATTGGGTTCA GAGGCCCTGT TGGTCAGGCT GGACCACCTG GATTTAAAGG TGAGCCAGGG
    GCTCCAGGGC CTCCAGGAGC TCAGGGAGTC CAGGGAATTC GTGGCAATGC CGGCACCCCC
    GGGTCACAAG GGGAAAGAGG GGCTCCAGGT CTGCCTGGGA TGCCAGGACA AAAGGGAGAG
    CGTGGGAAGA GAGGACGGAA TGGATTTGCT GGGCCCTCAG GACCATCAGG ATCCCCAGGA
    AAAGAGGGGA TTTCTGGGAC ACCTGGACCC AAAGGCAACA AGGGAGAAAT CGGCGTTGGA
    GCAGCTGGCC CAAGAGGTCC TCGTGGACTG CCTGGCCCTC GGGGTGACGA GGGCATCGTG
    GGAGTCCGTG GCCCCACCGG CATGACGGGT CTAAAAGGCT TCCCGGGTGT GAAGGGCGAA
    AGAGGTGATC GGGGACTTCT GGGACCCAAA GGAGAAAGAG GTGAGCCCAT GACTATTTTT
    GGACCCCAAG GCTATAAAGG CAGTAAAGGA GATCCTGGTG AACGGGGCCT GCCAGGTTTT
    GACGGAGACA AAGGCGAGAA AGGAGAGGAT GGGCCTATAG GAGAAAAGGG AGTCAAAGGT
    GAAGCTGGCT CCAAGGGAGT GATGGGGCTT TTTGGAACAA GAGGACCAGT GGGACAGAAG
    GGGGAGCCAG GAGAACCAGG CTTGCCGGGC TCTGCTGGTG CAGCAGGTCT TAATGGGAAG
    AACGGAAACA AGGGAGCCAA AGGTGACAGA GGTCTTCAGG GGCAGAAGGG GAAGCCGGGA
    GGAAAAGGTG ATCCTGGGGC CACTGGTGAC ATTGGACAGA AAGGAACAAA GGGCTTACGA
    GGGCTTCCAG GACGCATCGG TGCTCCTGGA GCTGAAGGCA TGAAGGGAGA AATTGGCAGT
    CCTGGAAAAC CAGGAATCCC AGGATCAGAT GGACCACACG GACAAAAGGG TGAAAAGGGA
    ACATCAGGAG ACGATGGTGC CTCGGGGATT CCTGGGGAGA AAGGAGAAAA GGGAGCCAAA
    GGAGTTCCTG GCTTGGGAGG CTTCAAAGGA CAAATGGGAT GGCCTGGGAG GACTGGAGTT
    GCTGGACCAG ATGGCCCTCA AGGGCCACAG GGTGAACCAG GGCCCCAGGG TCCACGAGGG
    AAAAGAGGGA GACCTCAGCT CTGCCTCAGG GGACCCCCAG GCATGGATGG GAGAAAAGGA
    GAAATGGGTG AAAATGGTCC AGCCGGCCAG AAAGGAGAGA AGGGAGATCC TGGTCTTTCT
    GAGGAAGAGG TGAAGGAGGT GGTCAGGAGC GAAATGAGAG GCAGATGCGA TTGTGGAGGC
    GAGTTGGGAA GGACACTCAT GGAAAGGGGC CTGGAGGCAA CTGAAATCAA CAGGCAGCTC
    CAAGGGTTCC AAAAGTTCCA GCTTTGGGAA CGCACCAGAA GGGATGGTGA AAGCCAGGAA
    GAAATGCTGA ACAAAACCCA GGGGATGACC ATGTTGATGA CAATGGTGAA GCCCGTCATG
    GAGCCACCTT CACTTATTGC AGCGGCTCCC CGGGCGCAGC AGATAACAGG TCCCTCTCAG
    CGCAAGCGGC GAGAAGCCCA CAGTCAATTA CCAGCCCCCT GCCTGCAGCC GATGGATGAA
    GGCTCCTGCC AGCACTATGC CCTGCTCTGG TATTACCACC TGGAGGCAAA CACCTGCCGG
    CCCTTCATCT TTGGGGGATG CCGAGGGAAC AGCAACCGCT TCGAAACCAA GTGGAAGTGT
    GAGCAGAAGT GCAAAACCTC AGCAGCCAGA GGGAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014816504.1
    CDS249..7727
    Translation

    Target ORF information:

    RefSeq Version XM_014961018.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax collagen alpha-1(VII) chain-like (LOC106899270), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014961018.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGTTTTGTG CCCAGATCCT TGCCCTGGCA GTGGCTGTGG TGCAGCACAG AGCTGTGGCC 
    GTTCATGAAG CATGCAGGAC GGCGGAGGTG GCCGACATTC TGTTCCTCGT GGGACAGTCA
    CAGGGCACAG GAAGGGAGAG CTCCTGGCTG GTCAAGGACT TCATCAGCAG CATGGCTCGT
    TCCTTTGAGA ACGTGGTGAT GGGCAAAGGA GGCATCCGGC TGGCAGTGGC ACTCTACGGG
    GAGAAACCAA GGATGAGTAT TGAGCTTACG GATTACGTGA CCATCGAAGA AATGTTGGTG
    GCAATTCAGG ATCTCTCCTT CAAAGGTGGA AGCTTAAAAA CAGGGTCAGC CTTGGCCTTT
    GCAGCTCGTG CCATGTCTCG TCTGGACACA CTGCGAGAGG ATGCAGCAAA GGTGGTTGTT
    TTGATCACGG ATGGAAAATC CAGCAACTCA GTTGAAGATG AAGCCCGGGT CCTGCAGGAT
    TTGGGGGTGA CGGTGTTTGC CGTAGGGATT AAGGGTGCTG ACAAGAATGA ATTGAACACA
    ATAGCTTCAG AGCCCACTGC AGAGCATGTG CTTTATGTTG AAGATTTCCA CCTCCTCCAT
    AACGTGGCCC CCAAACTCTC TCGAAGGCTC TGTTTCACTG CCTCGGAGCC ACCACGACCC
    ATCAAACAGA CTGTGCAAGC TGAGAAGATC GTCGGCCCCC GGGATTTGTA TGTCAGTGAG
    CACAGCCACA GCACGCTGAG ACTCACCTGG ATGCCAGCCA CGGGGAAGGT GACGGGCTAC
    CATGTCCATC TGCATCCCTT GCTGCCTTCA GGGCAGCCCG TTTCAGAAGA CCAGCAGCAG
    ATTGTGGTGG ACGGTGACAA AAGTACCGTG CTGGTGACTG ATCTGAAACC CAACACCAAG
    TATGTCTTCA CGGTGAGGGC CATCTATGCA GATACTGTTG GGGAGTCAGC GGTTGTCAAG
    GGGAAAACAA CACCTGTGCC TCCAGTCACT AATTTCCGTG TGATAGAAGA AGGACTTTTT
    AGCTTGAAGG TGGCTTGGAC ACCTCCGCTT GGCAAGCTGG AGGGCTACAA GATCTACATC
    CCCAGAGCCA ACAGACCAGG AATGACCTAC GAGCAGGTTC TGCGGGGTGA TGTCTCATCC
    CACGTGCTGG AGAACTTGCA GGAGGACAGA GAATACAGCA TCAGCATCTA CGCCGTGTAC
    CCCCAGGGCC CCAGCCAGCC TGTGGCTGCT GTGGGGAGAA CATTGAAGCT CCTGCCCGTG
    AAAAGCATCT TGCTGCAGAA TGAGACCACC AACACGCTCC AGGCAAGGTG GACACCCATC
    CGGGGAGCAA CGGGGTACAG GCTCACCTGG ACATCAGCAG AGGGCAGCAT CCAAGATGTG
    AACCTCAGCA ACACCTACAG CTACTACATG ATCCAGGGGC TGCAGCCTGG CACGGAGTAC
    ACTGTTACCA TCAACCCCAT CTTTGGGGAC GTCGAGGGAC CAGTGGTGAG CAGCAAAGCA
    ACGACGGTGG CATCGAGCAC CGTCCAGATG CTGAAAGTGA CTGAGATATC TACCAACAGT
    GCTCTGGTCT CCTGGGACTC AGTTGCTGGG GCCACTGGGT ATCGAGTGGC TTGGGGTCCT
    ACACCAGAGT TCTTTGGCAA AGACCGTCCT CGCCAGCTGG CCCTCAACAG GTCAATCACA
    GCCTACCAGC TCCGCAACCT GGCCCATGAC ACCGAGTACG TGATTTCCCT CTACGTGCTC
    TTTGGCTCCG TGGAAGGACC AGGGATCACA ACCTCAGCCA GGACATCTCC TCTTGGCTAC
    GTCTCCAATT TCAAGGTGAT GTCCTACACC AGCACAAGCC TGTCTCTGGT GTGGAGTGCC
    ACAGCAGCTG CCACCAAATT CAAAGTCACC TGGAGCCCTG TGGGAGCAGA AAAAGAAAAA
    CAGGTTGCCA AGACTCAGTA CCTGGGCAGT AGGGTGCTGG CTTACCAGAT TGACCAGTTA
    CTGCCCAACA CCCTGTATAA AGTCAGCGTT CGGGCTGTCT TCAACAAGAC TGAGGGACCA
    GAGGTGACTC TGACTCACCG CACAGCGCCT CTTGCAGACT CCAACCCCAT CCAGGCCGTT
    GAAGATCTGA GGATTACTGA CACCGGCGTA AATAGCTTGA AGCTGTCTTG GAGGAGACTT
    CCTGGGGTAA CTCATTATAA GATATCCTGG GCGCCGTTCA ATGGTGGCTT GGAAACCTCC
    CAGGTGGTTG CAGCAGAGAC GGTCTCCTTC ACGATCTCCA ACCTGAAGGA GAGCACCACC
    TACACCATCT GCATGTCTGC AATGATCGGG GGACAGGAGG GAAGCCCCAC ACTCCTCACA
    GCCAAAACTT TGGATTTACC CAAAGTGACT GAGTTCATGG TGCAGGAGGC TGCGGAGACC
    AGCGTTTTGC TGACATGGAC AGCTGTTCCT GGGGCATCCA CTTATCTATT AACATGGCGC
    CTGTCCTCAG CTTCTGATCT TTCCCAGGAA CTGCTGTCAG CAGCTTCTAG ATCGTATCGA
    GTGACAGGAC TGAGCACCAA AGAAATGTAT GTGTTTTCCA TAAGACCAGT CTTTGGAGAT
    ACGGAGGGAC CAGAGACAAC ACTCCTTGGG CAAACAGCGG GACCTCACAG AGAGTCACCC
    ACACCCATCA CTCCAGTGAC TTCTACAAAA CTGGGTACGA TGAGATTCCC ATCCTTATCT
    CCTAGCAAGG TGGGGACCAC TCCTCTTCCA CTGGCCACCA CCACAACAGT ACCACCAACT
    CCAGCAACCC TACTGTCGAC CACTGCAGTG TCCACACTGC TGACAACTCT CCCTGGGCCA
    ATCTGTGGCA AGTTCAAAGC AGACATTGGG TTCCTGGTGG ACGAGTCCTC GAGTATTGGC
    CAGAGCAATT TCAACAAAGT GAAGGATTTC CTCTTCCGGA TCATTTCCTA TTTCCCGAAG
    ATTGGGCCTG AAGGGACACA GGTTGCTGTT GCTCAGTACA GCGAGGAGCC CAGGGCAGCC
    TTCTACTTCA ACCAGCACCG GGACCGCAAC AGTGCCCTGA AAGCCGTCAA GGGACTGAGC
    TACGCTGGGG GCAACACAAA AACAGGTCGT GGCATAACCT ACATGCTCAA GGAGTTATTT
    CAGTCCTCCA GAGGGATGAG GCCAGATTTC CCTCACGTGC TGGTGCTGGT GACCGATGGG
    CGATCCCAGG ATGATGTGTT GCTACCAGCC AGAGCAGCCC ATGAGTTGGG GATCCGCGTC
    ATTGCTGTGG GTGTCTCAGG AGCTGACCCC GTTGAGCTCA ACAGCATCTT GCTGCAGCAG
    AACCTCCAGA ATGTTTTCTA CGTCAGCACG TTTGATGACT TCCCCCAGAT CCTCCGAGAG
    CTGATAGAGG TCATCTGTTC TGACCCCGAG CCTTCAGGGC CTCAACTACC ATCTGGGGAG
    AGCTTCAGGA GTTCACCAGC TCCTCATGGA GGCTATGACC CATACAGCTT TACTACAAAG
    GGAGAGAAAG GAGAGAGGGG TCTACCTGGG AAGGATGGAA TTCCCGGGCT GCCGGGCAGA
    CCGGGGCGGA CAGGTCCTCC TGGTCCCCCT GGACTCATGG GTCTCCCTGG CATCCAAGGT
    GATACTGGCT CTCCTGGCTA TCCTGGACCA GCGGGGCCAA AAGGAGAGAG AGGCGAACCA
    GGCTATGTCC TGGGAGGAGT AGAGGTGATT CCTGGCCGAA ATGGGCAGCC TGGCCCCCCT
    GGACAGAAAG GGCAGCCGGG GGTTCCTGGG GTAGCAGGAC CACCAGGTTT ACCAGGGCCA
    CAGGGGCCAC CAGGCATCTC CATCAAGGGT GAACGTGGGG ATTCAGGTAC CAGGGGCCCT
    CGTGGGAGGA ATGGCCTCAA GGGTGACAGA GGAGGAATGG GAGAGCCGGG GAAACCTGGG
    TTGCCAGGCC CAGTAGGGCT AGATGGTGTT CCTGGATTGC CTGGACCAAG GGGAGAAAAG
    GGTGTGATGG GACCTCCAGG AGCACCAGGA CCAAAGGGTA ACAAAGGGCA AGGTGGTGTG
    AAAGGGGAAA AGGGACTCCC AGGACCTCCA GGGCAGAAGG GAGACCAGGG GAACCCAGGA
    TTTCCAGGTG CTCCAGCTAT GGGGGTTTTG GGACCTCCAG GCAAGAAAGG TGTCAGGGGT
    GATATTGGAC CAACAGGGGC CCTGGGACCA CAAGGAGACA AAGGAATACA AGGAGACAAA
    GGAGAGAAGG GAAGCCCAGG TTTCGGGATT CCTGGTCAAC CTGGGCTGAA GGGAGATCCA
    GGCGACAGGG GAAATGTAGG TTTGTCGGGC AAACCAGGTC AGAAGGGGGA TAGAGGTCTG
    AAGGGAGAGA AAGGTGAACC AGGACTACCT GGGAAACCAG GCGAGACCGG ACTAAGAGGT
    AAAGATGGAG AAGCTGGTGT CCCTGGAGAA CCAGGAGAGA GAGGAATCAG GGGGCCACCT
    GGACTCCCAG GACGGCCTGG AGAGCAAGGG ATAAAAGGCG ATACAGGACC ACCTGGAAAG
    GATGGAATCA CTGGAGAGAA GGGAGACAAG GGCGAAGCTG GTGAGCCTGG GCTGCCTGGG
    ACTCCTGGAA GCCTTGTGTC TTCACTGGAG AGCACCATCA CCCTGAAAGG CGACAAGGGG
    GATCGTGGGA AGGATGGCTT GAAAGGCGAA AGAGGTCCTC CAGGCCCAAA GGGAGAACCC
    GGTCCACCAG GCAAAGGAGT GGAAATGAAG GATCTGGAGA GGCTTTTTGA AGCAAATGGT
    ATCAAGCTGG CACTGCTGAA GGACCTCTCC AATGCACTCC TGCAGGATGG GCTGGATGGG
    CTGGTGTGGC AGCTGGGTGG CTCCAGGACA AGCAAGACCT CCAGGAGAAA GCAAGCAGTT
    CTTCATGCTA CTGAGCATGG CGATTCACTG GTGTTTGATA CTTCTCCTGG TGCCCTGGCC
    AGCACTGAGG TGACAGAAGA AGCACAGAGC CTGGAGGTGG AGGCTCAGTT TAGGGTGCCG
    GTGTCTGGAG GACTCCTTAA GGGTCCATCT GCTGGTGGTT CTGAACCTGA GTACCAGCCC
    CTAGGAAACC CTCCTGAGTC CTTGGAGAGA ACATCTGAGG AAGGAAAGGA GGAGAGAGAC
    CCAAGATGGA CAATCACTTC TCTCAGTGGT AGCTTGGCTT TGCCCCCACT GCACCCAGAA
    GATGATCTGC AAGGAAACCA GTCCATGGAG CTTCTTGAGT CCCTGGAGGG GAGAGTGGAA
    GGACAACCAC AGCAGGACAC TAGCCACGAA AATGAGAATT TAGACGACGG CTCTGCGCTC
    CCAAGGCACG TTAGAGCCCA GAGATCTGCT GAGGACCAGA GACACGTGGT AAAGACCCCA
    TTTCACCACG ACAGCTTCGG GCTGGCCTTT GTGCAAGAGC AGAGCCATCC GTGTGACCCA
    GGAGGAAACG GCGACCTTTC CTTGTTGGCT CCATCACAGG TTCATCCCCC TGGTGCTCCA
    GGAGCTCGGA AATCCCCATC TGGATCCACG GCATACCCAG GCCCTCATCA CCCAAGGAGA
    ATTAAGAAGG AGGAAGCTGG TCCACCAGAT CCACCATCCC ATGGCCAAAA GGGAGAAATG
    GGAGCTCCGG GAGTCCGTGG AGACAAAGGA GAAAAGGGGC AGCCTGGGGA GATGGGGGAA
    CCTGGAGAAA AGGGCATCAA AGGAGACAGA GGTGAAAATG GTCAAAAGGG AGAACCAGGA
    ATTGGGTTCA GAGGCCCTGT TGGTCAGGCT GGACCACCTG GATTTAAAGG TGAGCCAGGG
    GCTCCAGGGC CTCCAGGAGC TCAGGGAGTC CAGGGAATTC GTGGCAATGC CGGCACCCCC
    GGGTCACAAG GGGAAAGAGG GGCTCCAGGT CTGCCTGGGA TGCCAGGACA AAAGGGAGAG
    CGTGGGAAGA GAGGACGGAA TGGATTTGCT GGGCCCTCAG GACCATCAGG ATCCCCAGGA
    AAAGAGGGGA TTTCTGGGAC ACCTGGACCC AAAGGCAACA AGGGAGAAAT CGGCGTTGGA
    GCAGCTGGCC CAAGAGGTCC TCGTGGACTG CCTGGCCCTC GGGGTGACGA GGGCATCGTG
    GGAGTCCGTG GCCCCACCGG CATGACGGGT CTAAAAGGCT TCCCGGGTGT GAAGGGCGAA
    AGAGGTGATC GGGGACTTCT GGGACCCAAA GGAGAAAGAG GTGAGCCCAT GACTATTTTT
    GGACCCCAAG GCTATAAAGG CAGTAAAGGA GATCCTGGTG AACGGGGCCT GCCAGGTTTT
    GACGGAGACA AAGGCGAGAA AGGAGAGGAT GGGCCTATAG GAGAAAAGGG AGTCAAAGGT
    GAAGCTGGCT CCAAGGGAGT GATGGGGCTT TTTGGAACAA GAGGACCAGT GGGACAGAAG
    GGGGAGCCAG GAGAACCAGG CTTGCCGGGC TCTGCTGGTG CAGCAGGTCT TAATGGGAAG
    AACGGAAACA AGGGAGCCAA AGGTGACAGA GGTCTTCAGG GGCAGAAGGG GAAGCCGGGA
    GGAAAAGGTG ATCCTGGGGC CACTGGTGAC ATTGGACAGA AAGGAACAAA GGGCTTACGA
    GGGCTTCCAG GACGCATCGG TGCTCCTGGA GCTGAAGGCA TGAAGGGAGA AATTGGCAGT
    CCTGGAAAAC CAGGAATCCC AGGATCAGAT GGACCACACG GACAAAAGGG TGAAAAGGGA
    ACATCAGGAG ACGATGGTGC CTCGGGGATT CCTGGGGAGA AAGGAGAAAA GGGAGCCAAA
    GGAGTTCCTG GCTTGGGAGG CTTCAAAGGA CAAATGGGAT GGCCTGGGAG GACTGGAGTT
    GCTGGACCAG ATGGCCCTCA AGGGCCACAG GGTGAACCAG GGCCCCAGGG TCCACGAGGG
    AAAAGAGGGA GACCTCAGCT CTGCCTCAGG GGACCCCCAG GCATGGATGG GAGAAAAGGA
    GAAATGGGTG AAAATGGTCC AGCCGGCCAG AAAGGAGAGA AGGGAGATCC TGGTCTTTCT
    GAGGAAGAGG TGAAGGAGGT GGTCAGGAGC GAAATGAGAG GCAGATGCGA TTGTGGAGGC
    GAGTTGGGAA GGACACTCAT GGAAAGGGGC CTGGAGGCAA CTGAAATCAA CAGGCAGCTC
    CAAGGGTTCC AAAAGTTCCA GCTTTGGGAA CGCACCAGAA GGGATGGTGA AAGCCAGGAA
    GAAATGCTGA ACAAAACCCA GGGGATGACC ATGTTGATGA CAATGGTGAA GCCCGTCATG
    GAGCCACCTT CACTTATTGC AGCGGCTCCC CGGGCGCAGC AGATAACAGG TCCCTCTCAG
    CGCAAGCGGC GAGAAGCCCA CAGTCAATTA CCAGCCCCCT GCCTGCAGCC GATGGATGAA
    GGCTCCTGCC AGCACTATGC CCTGCTCTGG TATTACCACC TGGAGGCAAA CACCTGCCGG
    CCCTTCATCT TTGGGGGATG CCGAGGGAAC AGCAACCGCT TCGAAACCAA GTGGAAGTGT
    GAGCAGAAGT GCAAAACCTC AGCAGCCAGA GGGAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa78936
    Clone ID Related Accession (Same CDS sequence) XM_014961019.1
    Accession Version XM_014961019.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7404bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product collagen alpha-1(VII) chain-like isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTTTTGTG CCCAGATCCT TGCCCTGGCA GTGGCTGTGG TGCAGCACAG AGCTGTGGCC 
    GTTCATGAAG CATGCAGGAC GGCGGAGGTG GCCGACATTC TGTTCCTCGT GGGACAGTCA
    CAGGGCACAG GAAGGGAGAG CTCCTGGCTG GTCAAGGACT TCATCAGCAG CATGGCTCGT
    TCCTTTGAGA ACGTGGTGAT GGGCAAAGGA GGCATCCGGC TGGCAGTGGC ACTCTACGGG
    GAGAAACCAA GGATGAGTAT TGAGCTTACG GATTACGTGA CCATCGAAGA AATGTTGGTG
    GCAATTCAGG ATCTCTCCTT CAAAGGTGGA AGCTTAAAAA CAGGGTCAGC CTTGGCCTTT
    GCAGCTCGTG CCATGTCTCG TCTGGACACA CTGCGAGAGG ATGCAGCAAA GGTGGTTGTT
    TTGATCACGG ATGGAAAATC CAGCAACTCA GTTGAAGATG AAGCCCGGGT CCTGCAGGAT
    TTGGGGGTGA CGGTGTTTGC CGTAGGGATT AAGGGTGCTG ACAAGAATGA ATTGAACACA
    ATAGCTTCAG AGCCCACTGC AGAGCATGTG CTTTATGTTG AAGATTTCCA CCTCCTCCAT
    AACGTGGCCC CCAAACTCTC TCGAAGGCTC TGTTTCACTG CCTCGGAGCC ACCACGACCC
    ATCAAACAGA CTGTGCAAGC TGAGAAGATC GTCGGCCCCC GGGATTTGTA TGTCAGTGAG
    CACAGCCACA GCACGCTGAG ACTCACCTGG ATGCCAGCCA CGGGGAAGGT GACGGGCTAC
    CATGTCCATC TGCATCCCTT GCTGCCTTCA GGGCAGCCCG TTTCAGAAGA CCAGCAGCAG
    ATTGTGGTGG ACGGTGACAA AAGTACCGTG CTGGTGACTG ATCTGAAACC CAACACCAAG
    TATGTCTTCA CGGTGAGGGC CATCTATGCA GATACTGTTG GGGAGTCAGC GGTTGTCAAG
    GGGAAAACAA CACCTGTGCC TCCAGTCACT AATTTCCGTG TGATAGAAGA AGGACTTTTT
    AGCTTGAAGG TGGCTTGGAC ACCTCCGCTT GGCAAGCTGG AGGGCTACAA GATCTACATC
    CCCAGAGCCA ACAGACCAGG AATGACCTAC GAGCAGGTTC TGCGGGGTGA TGTCTCATCC
    CACGTGCTGG AGAACTTGCA GGAGGACAGA GAATACAGCA TCAGCATCTA CGCCGTGTAC
    CCCCAGGGCC CCAGCCAGCC TGTGGCTGCT GTGGGGAGAA CATTGAAGCT CCTGCCCGTG
    AAAAGCATCT TGCTGCAGAA TGAGACCACC AACACGCTCC AGGCAAGGTG GACACCCATC
    CGGGGAGCAA CGGGGTACAG GCTCACCTGG ACATCAGCAG AGGGCAGCAT CCAAGATGTG
    AACCTCAGCA ACACCTACAG CTACTACATG ATCCAGGGGC TGCAGCCTGG CACGGAGTAC
    ACTGTTACCA TCAACCCCAT CTTTGGGGAC GTCGAGGGAC CAGTGGTGAG CAGCAAAGCA
    ACGACGGTGG CATCGAGCAC CGTCCAGATG CTGAAAGTGA CTGAGATATC TACCAACAGT
    GCTCTGGTCT CCTGGGACTC AGTTGCTGGG GCCACTGGGT ATCGAGTGGC TTGGGGTCCT
    ACACCAGAGT TCTTTGGCAA AGACCGTCCT CGCCAGCTGG CCCTCAACAG GTCAATCACA
    GCCTACCAGC TCCGCAACCT GGCCCATGAC ACCGAGTACG TGATTTCCCT CTACGTGCTC
    TTTGGCTCCG TGGAAGGACC AGGGATCACA ACCTCAGCCA GGACATCTCC TCTTGGCTAC
    GTCTCCAATT TCAAGGTGAT GTCCTACACC AGCACAAGCC TGTCTCTGGT GTGGAGTGCC
    ACAGCAGCTG CCACCAAATT CAAAGTCACC TGGAGCCCTG TGGGAGCAGA AAAAGAAAAA
    CAGGTTGCCA AGACTCAGTA CCTGGGCAGT AGGGTGCTGG CTTACCAGAT TGACCAGTTA
    CTGCCCAACA CCCTGTATAA AGTCAGCGTT CGGGCTGTCT TCAACAAGAC TGAGGGACCA
    GAGGTGACTC TGACTCACCG CACAGCGCCT CTTGCAGACT CCAACCCCAT CCAGGCCGTT
    GAAGATCTGA GGATTACTGA CACCGGCGTA AATAGCTTGA AGCTGTCTTG GAGGAGACTT
    CCTGGGGTAA CTCATTATAA GATATCCTGG GCGCCGTTCA ATGGTGGCTT GGAAACCTCC
    CAGGTGGTTG CAGCAGAGAC GGTCTCCTTC ACGATCTCCA ACCTGAAGGA GAGCACCACC
    TACACCATCT GCATGTCTGC AATGATCGGG GGACAGGAGG GAAGCCCCAC ACTCCTCACA
    GCCAAAACTT TGGATTTACC CAAAGTGACT GAGTTCATGG TGCAGGAGGC TGCGGAGACC
    AGCGTTTTGC TGACATGGAC AGCTGTTCCT GGGGCATCCA CTTATCTATT AACATGGCGC
    CTGTCCTCAG CTTCTGATCT TTCCCAGGAA CTGCTGTCAG CAGCTTCTAG ATCGTATCGA
    GTGACAGGAC TGAGCACCAA AGAAATGTAT GTGTTTTCCA TAAGACCAGT CTTTGGAGAT
    ACGGAGGGAC CAGAGACAAC ACTCCTTGGG CAAACAGCGG GACCTCACAG AGAGTCACCC
    ACACCCATCA CTCCAGTGAC TTCTACAAAA CTGGGTACGA TGAGATTCCC ATCCTTATCT
    CCTAGCAAGG TGGGGACCAC TCCTCTTCCA CTGGCCACCA CCACAACAGT ACCACCAACT
    CCAGCAACCC TACTGTCGAC CACTGCAGTG TCCACACTGC TGACAACTCT CCCTGGGCCA
    ATCTGTGGCA AGTTCAAAGC AGACATTGGG TTCCTGGTGG ACGAGTCCTC GAGTATTGGC
    CAGAGCAATT TCAACAAAGT GAAGGATTTC CTCTTCCGGA TCATTTCCTA TTTCCCGAAG
    ATTGGGCCTG AAGGGACACA GGTTGCTGTT GCTCAGTACA GCGAGGAGCC CAGGGCAGCC
    TTCTACTTCA ACCAGCACCG GGACCGCAAC AGTGCCCTGA AAGCCGTCAA GGGACTGAGC
    TACGCTGGGG GCAACACAAA AACAGGTCGT GGCATAACCT ACATGCTCAA GGAGTTATTT
    CAGTCCTCCA GAGGGATGAG GCCAGATTTC CCTCACGTGC TGGTGCTGGT GACCGATGGG
    CGATCCCAGG ATGATGTGTT GCTACCAGCC AGAGCAGCCC ATGAGTTGGG GATCCGCGTC
    ATTGCTGTGG GTGTCTCAGG AGCTGACCCC GTTGAGCTCA ACAGCATCTT GCTGCAGCAG
    AACCTCCAGA ATGTTTTCTA CGTCAGCACG TTTGATGACT TCCCCCAGAT CCTCCGAGAG
    CTGATAGAGG TCATCTGTTC TGACCCCGAG CCTTCAGGGC CTCAACTACC ATCTGGGGAG
    AGCTTCAGGA GTTCACCAGC TCCTCATGGA GGCTATGACC CATACAGCTT TACTACAAAG
    GGAGAGAAAG GAGAGAGGGG TCTACCTGGG AAGGATGGAA TTCCCGGGCT GCCGGGCAGA
    CCGGGGCGGA CAGGTCCTCC TGGTCCCCCT GGACTCATGG GTCTCCCTGG CATCCAAGGT
    GATACTGGCT CTCCTGGCTA TCCTGGACCA GCGGGGCCAA AAGGAGAGAG AGGCGAACCA
    GGCTATGTCC TGGGAGGAGT AGAGGTGATT CCTGGCCGAA ATGGGCAGCC TGGCCCCCCT
    GGACAGAAAG GGCAGCCGGG GGTTCCTGGG GTAGCAGGAC CACCAGGTTT ACCAGGGCCA
    CAGGGGCCAC CAGGCATCTC CATCAAGGGT GAACGTGGGG ATTCAGGTAC CAGGGGCCCT
    CGTGGGAGGA ATGGCCTCAA GGGTGACAGA GGAGGAATGG GAGAGCCGGG GAAACCTGGG
    TTGCCAGGCC CAGTAGGGCT AGATGGTGTT CCTGGATTGC CTGGACCAAG GGGAGAAAAG
    GGTGTGATGG GACCTCCAGG AGCACCAGGA CCAAAGGGTA ACAAAGGGCA AGGTGGTGTG
    AAAGGGGAAA AGGGACTCCC AGGACCTCCA GGGCAGAAGG GAGACCAGGG GAACCCAGGA
    TTTCCAGGTG CTCCAGCTAT GGGGGTTTTG GGACCTCCAG GCAAGAAAGG TGTCAGGGGT
    GATATTGGAC CAACAGGGGC CCTGGGACCA CAAGGAGACA AAGGAATACA AGGAGACAAA
    GGAGAGAAGG GAAGCCCAGG TTTCGGGATT CCTGGTCAAC CTGGGCTGAA GGGAGATCCA
    GGCGACAGGG GAAATGTAGG TTTGTCGGGC AAACCAGGTC AGAAGGGGGA TAGAGGTCTG
    AAGGGAGAGA AAGGTGAACC AGGACTACCT GGGAAACCAG GCGAGACCGG ACTAAGAGGT
    AAAGATGGAG AACCAGGGGA GAAAGGCACC TCTGGGACCA AGGGAGAAGC TGGTGTCCCT
    GGAGAACCAG GAGAGAGAGG AATCAGGGGG CCACCTGGAC TCCCAGGACG GCCTGGAGAG
    CAAGGGATAA AAGGCGATAC AGGACCACCT GGAAAGGATG GAATCACTGG AGAGAAGGGA
    GACAAGGGCG AAGCTGGTGA GCCTGGGCTG CCTGGGACTC CTGGAAGCCT TGTGTCTTCA
    CTGGAGAGCA CCATCACCCT GAAAGGCGAC AAGGGGGATC GTGGGAAGGA TGGCTTGAAA
    GGCGAAAGAG GTCCTCCAGG CCCAAAGGGA GAACCCGGTC CACCAGGCAA AGGAGTGGAA
    ATGAAGGATC TGGAGAGGCT TTTTGAAGCA AATGGTATCA AGCTGGCACT GCTGAAGGAC
    CTCTCCAATG CACTCCTGCA GGATGGGCTG GATGGGCTGG TGTGGCAGCT GGGTGGCTCC
    AGGACAAGCA AGACCTCCAG GAGAAAGCAA GCAGTTCTTC ATGCTACTGA GCATGGCGAT
    TCACTGGTGT TTGATACTTC TCCTGGTGCC CTGGCCAGCA CTGAGGTGAC AGAAGAAGCA
    CAGAGCCTGG AGGTGGAGGC TCAGTTTAGG GTGCCGGTGT CTGGAGGACT CCTTAAGGGT
    CCATCTGCTG GTGGTTCTGA ACCTGAGTAC CAGCCCCTAG GAAACCCTCC TGAGTCCTTG
    GAGAGAACAT CTGAGGAAGG AAAGGAGGAG AGAGACCCAA GATGGACAAT CACTTCTCTC
    AGTGGTAGCT TGGCTTTGCC CCCACTGCAC CCAGAAGATG ATCTGCAAGG AAACCAGTCC
    ATGGAGCTTC TTGAGTCCCT GGAGGGGAGA GTGGAAGGAC AACCACAGCA GGACACTAGC
    CACGAAAATG AGAATTTAGA CGACGGCTCT GCGCTCCCAA GGCACGTTAG AGCCCAGAGA
    TCTGCTGAGG ACCAGAGACA CGTGGTTCAT CCCCCTGGTG CTCCAGGAGC TCGGAAATCC
    CCATCTGGAT CCACGGCATA CCCAGGCCCT CATCACCCAA GGAGAATTAA GAAGGAGGAA
    GCTGGTCCAC CAGATCCACC ATCCCATGGC CAAAAGGGAG AAATGGGAGC TCCGGGAGTC
    CGTGGAGACA AAGGAGAAAA GGGGCAGCCT GGGGAGATGG GGGAACCTGG AGAAAAGGGC
    ATCAAAGGAG ACAGAGGTGA AAATGGTCAA AAGGGAGAAC CAGGAATTGG GTTCAGAGGC
    CCTGTTGGTC AGGCTGGACC ACCTGGATTT AAAGGTGAGC CAGGGGCTCC AGGGCCTCCA
    GGAGCTCAGG GAGTCCAGGG AATTCGTGGC AATGCCGGCA CCCCCGGGTC ACAAGGGGAA
    AGAGGGGCTC CAGGTCTGCC TGGGATGCCA GGACAAAAGG GAGAGCGTGG GAAGAGAGGA
    CGGAATGGAT TTGCTGGGCC CTCAGGACCA TCAGGATCCC CAGGAAAAGA GGGGATTTCT
    GGGACACCTG GACCCAAAGG CAACAAGGGA GAAATCGGCG TTGGAGCAGC TGGCCCAAGA
    GGTCCTCGTG GACTGCCTGG CCCTCGGGGT GACGAGGGCA TCGTGGGAGT CCGTGGCCCC
    ACCGGCATGA CGGGTCTAAA AGGCTTCCCG GGTGTGAAGG GCGAAAGAGG TGATCGGGGA
    CTTCTGGGAC CCAAAGGAGA AAGAGGTGAG CCCATGACTA TTTTTGGACC CCAAGGCTAT
    AAAGGCAGTA AAGGAGATCC TGGTGAACGG GGCCTGCCAG GTTTTGACGG AGACAAAGGC
    GAGAAAGGAG AGGATGGGCC TATAGGAGAA AAGGGAGTCA AAGGTGAAGC TGGCTCCAAG
    GGAGTGATGG GGCTTTTTGG AACAAGAGGA CCAGTGGGAC AGAAGGGGGA GCCAGGAGAA
    CCAGGCTTGC CGGGCTCTGC TGGTGCAGCA GGTCTTAATG GGAAGAACGG AAACAAGGGA
    GCCAAAGGTG ACAGAGGTCT TCAGGGGCAG AAGGGGAAGC CGGGAGGAAA AGGTGATCCT
    GGGGCCACTG GTGACATTGG ACAGAAAGGA ACAAAGGGCT TACGAGGGCT TCCAGGACGC
    ATCGGTGCTC CTGGAGCTGA AGGCATGAAG GGAGAAATTG GCAGTCCTGG AAAACCAGGA
    ATCCCAGGAT CAGATGGACC ACACGGACAA AAGGGTGAAA AGGGAACATC AGGAGACGAT
    GGTGCCTCGG GGATTCCTGG GGAGAAAGGA GAAAAGGGAG CCAAAGGAGT TCCTGGCTTG
    GGAGGCTTCA AAGGACAAAT GGGATGGCCT GGGAGGACTG GAGTTGCTGG ACCAGATGGC
    CCTCAAGGGC CACAGGGTGA ACCAGGGCCC CAGGGTCCAC GAGGGAAAAG AGGGAGACCT
    CAGCTCTGCC TCAGGGGACC CCCAGGCATG GATGGGAGAA AAGGAGAAAT GGGTGAAAAT
    GGTCCAGCCG GCCAGAAAGG AGAGAAGGGA GATCCTGGTC TTTCTGAGGA AGAGGTGAAG
    GAGGTGGTCA GGAGCGAAAT GAGAGGCAGA TGCGATTGTG GAGGCGAGTT GGGAAGGACA
    CTCATGGAAA GGGGCCTGGA GGCAACTGAA ATCAACAGGC AGCTCCAAGG GTTCCAAAAG
    TTCCAGCTTT GGGAACGCAC CAGAAGGGAT GGTGAAAGCC AGGAAGAAAT GCTGAACAAA
    ACCCAGGGGA TGACCATGTT GATGACAATG GTGAAGCCCG TCATGGAGCC ACCTTCACTT
    ATTGCAGCGG CTCCCCGGGC GCAGCAGATA ACAGGTCCCT CTCAGCGCAA GCGGCGAGAA
    GCCCACAGTC AATTACCAGC CCCCTGCCTG CAGCCGATGG ATGAAGGCTC CTGCCAGCAC
    TATGCCCTGC TCTGGTATTA CCACCTGGAG GCAAACACCT GCCGGCCCTT CATCTTTGGG
    GGATGCCGAG GGAACAGCAA CCGCTTCGAA ACCAAGTGGA AGTGTGAGCA GAAGTGCAAA
    ACCTCAGCAG CCAGAGGGAG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014816505.1
    CDS249..7652
    Translation

    Target ORF information:

    RefSeq Version XM_014961019.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax collagen alpha-1(VII) chain-like (LOC106899270), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014961019.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGTTTTGTG CCCAGATCCT TGCCCTGGCA GTGGCTGTGG TGCAGCACAG AGCTGTGGCC 
    GTTCATGAAG CATGCAGGAC GGCGGAGGTG GCCGACATTC TGTTCCTCGT GGGACAGTCA
    CAGGGCACAG GAAGGGAGAG CTCCTGGCTG GTCAAGGACT TCATCAGCAG CATGGCTCGT
    TCCTTTGAGA ACGTGGTGAT GGGCAAAGGA GGCATCCGGC TGGCAGTGGC ACTCTACGGG
    GAGAAACCAA GGATGAGTAT TGAGCTTACG GATTACGTGA CCATCGAAGA AATGTTGGTG
    GCAATTCAGG ATCTCTCCTT CAAAGGTGGA AGCTTAAAAA CAGGGTCAGC CTTGGCCTTT
    GCAGCTCGTG CCATGTCTCG TCTGGACACA CTGCGAGAGG ATGCAGCAAA GGTGGTTGTT
    TTGATCACGG ATGGAAAATC CAGCAACTCA GTTGAAGATG AAGCCCGGGT CCTGCAGGAT
    TTGGGGGTGA CGGTGTTTGC CGTAGGGATT AAGGGTGCTG ACAAGAATGA ATTGAACACA
    ATAGCTTCAG AGCCCACTGC AGAGCATGTG CTTTATGTTG AAGATTTCCA CCTCCTCCAT
    AACGTGGCCC CCAAACTCTC TCGAAGGCTC TGTTTCACTG CCTCGGAGCC ACCACGACCC
    ATCAAACAGA CTGTGCAAGC TGAGAAGATC GTCGGCCCCC GGGATTTGTA TGTCAGTGAG
    CACAGCCACA GCACGCTGAG ACTCACCTGG ATGCCAGCCA CGGGGAAGGT GACGGGCTAC
    CATGTCCATC TGCATCCCTT GCTGCCTTCA GGGCAGCCCG TTTCAGAAGA CCAGCAGCAG
    ATTGTGGTGG ACGGTGACAA AAGTACCGTG CTGGTGACTG ATCTGAAACC CAACACCAAG
    TATGTCTTCA CGGTGAGGGC CATCTATGCA GATACTGTTG GGGAGTCAGC GGTTGTCAAG
    GGGAAAACAA CACCTGTGCC TCCAGTCACT AATTTCCGTG TGATAGAAGA AGGACTTTTT
    AGCTTGAAGG TGGCTTGGAC ACCTCCGCTT GGCAAGCTGG AGGGCTACAA GATCTACATC
    CCCAGAGCCA ACAGACCAGG AATGACCTAC GAGCAGGTTC TGCGGGGTGA TGTCTCATCC
    CACGTGCTGG AGAACTTGCA GGAGGACAGA GAATACAGCA TCAGCATCTA CGCCGTGTAC
    CCCCAGGGCC CCAGCCAGCC TGTGGCTGCT GTGGGGAGAA CATTGAAGCT CCTGCCCGTG
    AAAAGCATCT TGCTGCAGAA TGAGACCACC AACACGCTCC AGGCAAGGTG GACACCCATC
    CGGGGAGCAA CGGGGTACAG GCTCACCTGG ACATCAGCAG AGGGCAGCAT CCAAGATGTG
    AACCTCAGCA ACACCTACAG CTACTACATG ATCCAGGGGC TGCAGCCTGG CACGGAGTAC
    ACTGTTACCA TCAACCCCAT CTTTGGGGAC GTCGAGGGAC CAGTGGTGAG CAGCAAAGCA
    ACGACGGTGG CATCGAGCAC CGTCCAGATG CTGAAAGTGA CTGAGATATC TACCAACAGT
    GCTCTGGTCT CCTGGGACTC AGTTGCTGGG GCCACTGGGT ATCGAGTGGC TTGGGGTCCT
    ACACCAGAGT TCTTTGGCAA AGACCGTCCT CGCCAGCTGG CCCTCAACAG GTCAATCACA
    GCCTACCAGC TCCGCAACCT GGCCCATGAC ACCGAGTACG TGATTTCCCT CTACGTGCTC
    TTTGGCTCCG TGGAAGGACC AGGGATCACA ACCTCAGCCA GGACATCTCC TCTTGGCTAC
    GTCTCCAATT TCAAGGTGAT GTCCTACACC AGCACAAGCC TGTCTCTGGT GTGGAGTGCC
    ACAGCAGCTG CCACCAAATT CAAAGTCACC TGGAGCCCTG TGGGAGCAGA AAAAGAAAAA
    CAGGTTGCCA AGACTCAGTA CCTGGGCAGT AGGGTGCTGG CTTACCAGAT TGACCAGTTA
    CTGCCCAACA CCCTGTATAA AGTCAGCGTT CGGGCTGTCT TCAACAAGAC TGAGGGACCA
    GAGGTGACTC TGACTCACCG CACAGCGCCT CTTGCAGACT CCAACCCCAT CCAGGCCGTT
    GAAGATCTGA GGATTACTGA CACCGGCGTA AATAGCTTGA AGCTGTCTTG GAGGAGACTT
    CCTGGGGTAA CTCATTATAA GATATCCTGG GCGCCGTTCA ATGGTGGCTT GGAAACCTCC
    CAGGTGGTTG CAGCAGAGAC GGTCTCCTTC ACGATCTCCA ACCTGAAGGA GAGCACCACC
    TACACCATCT GCATGTCTGC AATGATCGGG GGACAGGAGG GAAGCCCCAC ACTCCTCACA
    GCCAAAACTT TGGATTTACC CAAAGTGACT GAGTTCATGG TGCAGGAGGC TGCGGAGACC
    AGCGTTTTGC TGACATGGAC AGCTGTTCCT GGGGCATCCA CTTATCTATT AACATGGCGC
    CTGTCCTCAG CTTCTGATCT TTCCCAGGAA CTGCTGTCAG CAGCTTCTAG ATCGTATCGA
    GTGACAGGAC TGAGCACCAA AGAAATGTAT GTGTTTTCCA TAAGACCAGT CTTTGGAGAT
    ACGGAGGGAC CAGAGACAAC ACTCCTTGGG CAAACAGCGG GACCTCACAG AGAGTCACCC
    ACACCCATCA CTCCAGTGAC TTCTACAAAA CTGGGTACGA TGAGATTCCC ATCCTTATCT
    CCTAGCAAGG TGGGGACCAC TCCTCTTCCA CTGGCCACCA CCACAACAGT ACCACCAACT
    CCAGCAACCC TACTGTCGAC CACTGCAGTG TCCACACTGC TGACAACTCT CCCTGGGCCA
    ATCTGTGGCA AGTTCAAAGC AGACATTGGG TTCCTGGTGG ACGAGTCCTC GAGTATTGGC
    CAGAGCAATT TCAACAAAGT GAAGGATTTC CTCTTCCGGA TCATTTCCTA TTTCCCGAAG
    ATTGGGCCTG AAGGGACACA GGTTGCTGTT GCTCAGTACA GCGAGGAGCC CAGGGCAGCC
    TTCTACTTCA ACCAGCACCG GGACCGCAAC AGTGCCCTGA AAGCCGTCAA GGGACTGAGC
    TACGCTGGGG GCAACACAAA AACAGGTCGT GGCATAACCT ACATGCTCAA GGAGTTATTT
    CAGTCCTCCA GAGGGATGAG GCCAGATTTC CCTCACGTGC TGGTGCTGGT GACCGATGGG
    CGATCCCAGG ATGATGTGTT GCTACCAGCC AGAGCAGCCC ATGAGTTGGG GATCCGCGTC
    ATTGCTGTGG GTGTCTCAGG AGCTGACCCC GTTGAGCTCA ACAGCATCTT GCTGCAGCAG
    AACCTCCAGA ATGTTTTCTA CGTCAGCACG TTTGATGACT TCCCCCAGAT CCTCCGAGAG
    CTGATAGAGG TCATCTGTTC TGACCCCGAG CCTTCAGGGC CTCAACTACC ATCTGGGGAG
    AGCTTCAGGA GTTCACCAGC TCCTCATGGA GGCTATGACC CATACAGCTT TACTACAAAG
    GGAGAGAAAG GAGAGAGGGG TCTACCTGGG AAGGATGGAA TTCCCGGGCT GCCGGGCAGA
    CCGGGGCGGA CAGGTCCTCC TGGTCCCCCT GGACTCATGG GTCTCCCTGG CATCCAAGGT
    GATACTGGCT CTCCTGGCTA TCCTGGACCA GCGGGGCCAA AAGGAGAGAG AGGCGAACCA
    GGCTATGTCC TGGGAGGAGT AGAGGTGATT CCTGGCCGAA ATGGGCAGCC TGGCCCCCCT
    GGACAGAAAG GGCAGCCGGG GGTTCCTGGG GTAGCAGGAC CACCAGGTTT ACCAGGGCCA
    CAGGGGCCAC CAGGCATCTC CATCAAGGGT GAACGTGGGG ATTCAGGTAC CAGGGGCCCT
    CGTGGGAGGA ATGGCCTCAA GGGTGACAGA GGAGGAATGG GAGAGCCGGG GAAACCTGGG
    TTGCCAGGCC CAGTAGGGCT AGATGGTGTT CCTGGATTGC CTGGACCAAG GGGAGAAAAG
    GGTGTGATGG GACCTCCAGG AGCACCAGGA CCAAAGGGTA ACAAAGGGCA AGGTGGTGTG
    AAAGGGGAAA AGGGACTCCC AGGACCTCCA GGGCAGAAGG GAGACCAGGG GAACCCAGGA
    TTTCCAGGTG CTCCAGCTAT GGGGGTTTTG GGACCTCCAG GCAAGAAAGG TGTCAGGGGT
    GATATTGGAC CAACAGGGGC CCTGGGACCA CAAGGAGACA AAGGAATACA AGGAGACAAA
    GGAGAGAAGG GAAGCCCAGG TTTCGGGATT CCTGGTCAAC CTGGGCTGAA GGGAGATCCA
    GGCGACAGGG GAAATGTAGG TTTGTCGGGC AAACCAGGTC AGAAGGGGGA TAGAGGTCTG
    AAGGGAGAGA AAGGTGAACC AGGACTACCT GGGAAACCAG GCGAGACCGG ACTAAGAGGT
    AAAGATGGAG AACCAGGGGA GAAAGGCACC TCTGGGACCA AGGGAGAAGC TGGTGTCCCT
    GGAGAACCAG GAGAGAGAGG AATCAGGGGG CCACCTGGAC TCCCAGGACG GCCTGGAGAG
    CAAGGGATAA AAGGCGATAC AGGACCACCT GGAAAGGATG GAATCACTGG AGAGAAGGGA
    GACAAGGGCG AAGCTGGTGA GCCTGGGCTG CCTGGGACTC CTGGAAGCCT TGTGTCTTCA
    CTGGAGAGCA CCATCACCCT GAAAGGCGAC AAGGGGGATC GTGGGAAGGA TGGCTTGAAA
    GGCGAAAGAG GTCCTCCAGG CCCAAAGGGA GAACCCGGTC CACCAGGCAA AGGAGTGGAA
    ATGAAGGATC TGGAGAGGCT TTTTGAAGCA AATGGTATCA AGCTGGCACT GCTGAAGGAC
    CTCTCCAATG CACTCCTGCA GGATGGGCTG GATGGGCTGG TGTGGCAGCT GGGTGGCTCC
    AGGACAAGCA AGACCTCCAG GAGAAAGCAA GCAGTTCTTC ATGCTACTGA GCATGGCGAT
    TCACTGGTGT TTGATACTTC TCCTGGTGCC CTGGCCAGCA CTGAGGTGAC AGAAGAAGCA
    CAGAGCCTGG AGGTGGAGGC TCAGTTTAGG GTGCCGGTGT CTGGAGGACT CCTTAAGGGT
    CCATCTGCTG GTGGTTCTGA ACCTGAGTAC CAGCCCCTAG GAAACCCTCC TGAGTCCTTG
    GAGAGAACAT CTGAGGAAGG AAAGGAGGAG AGAGACCCAA GATGGACAAT CACTTCTCTC
    AGTGGTAGCT TGGCTTTGCC CCCACTGCAC CCAGAAGATG ATCTGCAAGG AAACCAGTCC
    ATGGAGCTTC TTGAGTCCCT GGAGGGGAGA GTGGAAGGAC AACCACAGCA GGACACTAGC
    CACGAAAATG AGAATTTAGA CGACGGCTCT GCGCTCCCAA GGCACGTTAG AGCCCAGAGA
    TCTGCTGAGG ACCAGAGACA CGTGGTTCAT CCCCCTGGTG CTCCAGGAGC TCGGAAATCC
    CCATCTGGAT CCACGGCATA CCCAGGCCCT CATCACCCAA GGAGAATTAA GAAGGAGGAA
    GCTGGTCCAC CAGATCCACC ATCCCATGGC CAAAAGGGAG AAATGGGAGC TCCGGGAGTC
    CGTGGAGACA AAGGAGAAAA GGGGCAGCCT GGGGAGATGG GGGAACCTGG AGAAAAGGGC
    ATCAAAGGAG ACAGAGGTGA AAATGGTCAA AAGGGAGAAC CAGGAATTGG GTTCAGAGGC
    CCTGTTGGTC AGGCTGGACC ACCTGGATTT AAAGGTGAGC CAGGGGCTCC AGGGCCTCCA
    GGAGCTCAGG GAGTCCAGGG AATTCGTGGC AATGCCGGCA CCCCCGGGTC ACAAGGGGAA
    AGAGGGGCTC CAGGTCTGCC TGGGATGCCA GGACAAAAGG GAGAGCGTGG GAAGAGAGGA
    CGGAATGGAT TTGCTGGGCC CTCAGGACCA TCAGGATCCC CAGGAAAAGA GGGGATTTCT
    GGGACACCTG GACCCAAAGG CAACAAGGGA GAAATCGGCG TTGGAGCAGC TGGCCCAAGA
    GGTCCTCGTG GACTGCCTGG CCCTCGGGGT GACGAGGGCA TCGTGGGAGT CCGTGGCCCC
    ACCGGCATGA CGGGTCTAAA AGGCTTCCCG GGTGTGAAGG GCGAAAGAGG TGATCGGGGA
    CTTCTGGGAC CCAAAGGAGA AAGAGGTGAG CCCATGACTA TTTTTGGACC CCAAGGCTAT
    AAAGGCAGTA AAGGAGATCC TGGTGAACGG GGCCTGCCAG GTTTTGACGG AGACAAAGGC
    GAGAAAGGAG AGGATGGGCC TATAGGAGAA AAGGGAGTCA AAGGTGAAGC TGGCTCCAAG
    GGAGTGATGG GGCTTTTTGG AACAAGAGGA CCAGTGGGAC AGAAGGGGGA GCCAGGAGAA
    CCAGGCTTGC CGGGCTCTGC TGGTGCAGCA GGTCTTAATG GGAAGAACGG AAACAAGGGA
    GCCAAAGGTG ACAGAGGTCT TCAGGGGCAG AAGGGGAAGC CGGGAGGAAA AGGTGATCCT
    GGGGCCACTG GTGACATTGG ACAGAAAGGA ACAAAGGGCT TACGAGGGCT TCCAGGACGC
    ATCGGTGCTC CTGGAGCTGA AGGCATGAAG GGAGAAATTG GCAGTCCTGG AAAACCAGGA
    ATCCCAGGAT CAGATGGACC ACACGGACAA AAGGGTGAAA AGGGAACATC AGGAGACGAT
    GGTGCCTCGG GGATTCCTGG GGAGAAAGGA GAAAAGGGAG CCAAAGGAGT TCCTGGCTTG
    GGAGGCTTCA AAGGACAAAT GGGATGGCCT GGGAGGACTG GAGTTGCTGG ACCAGATGGC
    CCTCAAGGGC CACAGGGTGA ACCAGGGCCC CAGGGTCCAC GAGGGAAAAG AGGGAGACCT
    CAGCTCTGCC TCAGGGGACC CCCAGGCATG GATGGGAGAA AAGGAGAAAT GGGTGAAAAT
    GGTCCAGCCG GCCAGAAAGG AGAGAAGGGA GATCCTGGTC TTTCTGAGGA AGAGGTGAAG
    GAGGTGGTCA GGAGCGAAAT GAGAGGCAGA TGCGATTGTG GAGGCGAGTT GGGAAGGACA
    CTCATGGAAA GGGGCCTGGA GGCAACTGAA ATCAACAGGC AGCTCCAAGG GTTCCAAAAG
    TTCCAGCTTT GGGAACGCAC CAGAAGGGAT GGTGAAAGCC AGGAAGAAAT GCTGAACAAA
    ACCCAGGGGA TGACCATGTT GATGACAATG GTGAAGCCCG TCATGGAGCC ACCTTCACTT
    ATTGCAGCGG CTCCCCGGGC GCAGCAGATA ACAGGTCCCT CTCAGCGCAA GCGGCGAGAA
    GCCCACAGTC AATTACCAGC CCCCTGCCTG CAGCCGATGG ATGAAGGCTC CTGCCAGCAC
    TATGCCCTGC TCTGGTATTA CCACCTGGAG GCAAACACCT GCCGGCCCTT CATCTTTGGG
    GGATGCCGAG GGAACAGCAA CCGCTTCGAA ACCAAGTGGA AGTGTGAGCA GAAGTGCAAA
    ACCTCAGCAG CCAGAGGGAG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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