LOC106899270 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC106899270
Entrez Gene ID	106899270
Full Name	collagen alpha-1(VII) chain-like
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014961016.1	XP_014816502.1	collagen alpha-1(VII) chain-like isoform X2
XM_014961017.1	XP_014816503.1	collagen alpha-1(VII) chain-like isoform X3
XM_014961019.1	XP_014816505.1	collagen alpha-1(VII) chain-like isoform X5
XM_014961015.1	XP_014816501.1	collagen alpha-1(VII) chain-like isoform X1
XM_014961018.1	XP_014816504.1	collagen alpha-1(VII) chain-like isoform X4

Pathways from BioSystems
Gene Ontology

The following LOC106899270 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC106899270 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa78934	XM_014961016.1 Latest version!	Calidris pugnax collagen alpha-1(VII) chain-like (LOC106899270), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCa78934	XM_014961015.1 Latest version!	Calidris pugnax collagen alpha-1(VII) chain-like (LOC106899270), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCa78934	XM_014961017.1 Latest version!	Calidris pugnax collagen alpha-1(VII) chain-like (LOC106899270), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCa78935	XM_014961018.1 Latest version!	Calidris pugnax collagen alpha-1(VII) chain-like (LOC106899270), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OCa78936	XM_014961019.1 Latest version!	Calidris pugnax collagen alpha-1(VII) chain-like (LOC106899270), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC106899270 ( XM_014961016.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014816502.1 Calidris pugnax collagen alpha-1(VII) chain-like (LOC106899270), transcript variant X2, mRNA.

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CloneID	OCa78934
Clone ID Related Accession (Same CDS sequence)	XM_014961016.1 , XM_014961017.1 , XM_014961015.1
Accession Version	XM_014961016.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	collagen alpha-1(VII) chain-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTGTG CCCAGATCCT TGCCCTGGCA GTGGCTGTGG TGCAGCACAG AGCTGTGGCC 
GTTCATGAAG CATGCAGGAC GGCGGAGGTG GCCGACATTC TGTTCCTCGT GGGACAGTCA 
CAGGGCACAG GAAGGGAGAG CTCCTGGCTG GTCAAGGACT TCATCAGCAG CATGGCTCGT 
TCCTTTGAGA ACGTGGTGAT GGGCAAAGGA GGCATCCGGC TGGCAGTGGC ACTCTACGGG 
GAGAAACCAA GGATGAGTAT TGAGCTTACG GATTACGTGA CCATCGAAGA AATGTTGGTG 
GCAATTCAGG ATCTCTCCTT CAAAGGTGGA AGCTTAAAAA CAGGGTCAGC CTTGGCCTTT 
GCAGCTCGTG CCATGTCTCG TCTGGACACA CTGCGAGAGG ATGCAGCAAA GGTGGTTGTT 
TTGATCACGG ATGGAAAATC CAGCAACTCA GTTGAAGATG AAGCCCGGGT CCTGCAGGAT 
TTGGGGGTGA CGGTGTTTGC CGTAGGGATT AAGGGTGCTG ACAAGAATGA ATTGAACACA 
ATAGCTTCAG AGCCCACTGC AGAGCATGTG CTTTATGTTG AAGATTTCCA CCTCCTCCAT 
AACGTGGCCC CCAAACTCTC TCGAAGGCTC TGTTTCACTG CCTCGGAGCC ACCACGACCC 
ATCAAACAGA CTGTGCAAGC TGAGAAGATC GTCGGCCCCC GGGATTTGTA TGTCAGTGAG 
CACAGCCACA GCACGCTGAG ACTCACCTGG ATGCCAGCCA CGGGGAAGGT GACGGGCTAC 
CATGTCCATC TGCATCCCTT GCTGCCTTCA GGGCAGCCCG TTTCAGAAGA CCAGCAGCAG 
ATTGTGGTGG ACGGTGACAA AAGTACCGTG CTGGTGACTG ATCTGAAACC CAACACCAAG 
TATGTCTTCA CGGTGAGGGC CATCTATGCA GATACTGTTG GGGAGTCAGC GGTTGTCAAG 
GGGAAAACAA CACCTGTGCC TCCAGTCACT AATTTCCGTG TGATAGAAGA AGGACTTTTT 
AGCTTGAAGG TGGCTTGGAC ACCTCCGCTT GGCAAGCTGG AGGGCTACAA GATCTACATC 
CCCAGAGCCA ACAGACCAGG AATGACCTAC GAGCAGGTTC TGCGGGGTGA TGTCTCATCC 
CACGTGCTGG AGAACTTGCA GGAGGACAGA GAATACAGCA TCAGCATCTA CGCCGTGTAC 
CCCCAGGGCC CCAGCCAGCC TGTGGCTGCT GTGGGGAGAA CATTGAAGCT CCTGCCCGTG 
AAAAGCATCT TGCTGCAGAA TGAGACCACC AACACGCTCC AGGCAAGGTG GACACCCATC 
CGGGGAGCAA CGGGGTACAG GCTCACCTGG ACATCAGCAG AGGGCAGCAT CCAAGATGTG 
AACCTCAGCA ACACCTACAG CTACTACATG ATCCAGGGGC TGCAGCCTGG CACGGAGTAC 
ACTGTTACCA TCAACCCCAT CTTTGGGGAC GTCGAGGGAC CAGTGGTGAG CAGCAAAGCA 
ACGACGGTGG CATCGAGCAC CGTCCAGATG CTGAAAGTGA CTGAGATATC TACCAACAGT 
GCTCTGGTCT CCTGGGACTC AGTTGCTGGG GCCACTGGGT ATCGAGTGGC TTGGGGTCCT 
ACACCAGAGT TCTTTGGCAA AGACCGTCCT CGCCAGCTGG CCCTCAACAG GTCAATCACA 
GCCTACCAGC TCCGCAACCT GGCCCATGAC ACCGAGTACG TGATTTCCCT CTACGTGCTC 
TTTGGCTCCG TGGAAGGACC AGGGATCACA ACCTCAGCCA GGACATCTCC TCTTGGCTAC 
GTCTCCAATT TCAAGGTGAT GTCCTACACC AGCACAAGCC TGTCTCTGGT GTGGAGTGCC 
ACAGCAGCTG CCACCAAATT CAAAGTCACC TGGAGCCCTG TGGGAGCAGA AAAAGAAAAA 
CAGGTTGCCA AGACTCAGTA CCTGGGCAGT AGGGTGCTGG CTTACCAGAT TGACCAGTTA 
CTGCCCAACA CCCTGTATAA AGTCAGCGTT CGGGCTGTCT TCAACAAGAC TGAGGGACCA 
GAGGTGACTC TGACTCACCG CACAGCGCCT CTTGCAGACT CCAACCCCAT CCAGGCCGTT 
GAAGATCTGA GGATTACTGA CACCGGCGTA AATAGCTTGA AGCTGTCTTG GAGGAGACTT 
CCTGGGGTAA CTCATTATAA GATATCCTGG GCGCCGTTCA ATGGTGGCTT GGAAACCTCC 
CAGGTGGTTG CAGCAGAGAC GGTCTCCTTC ACGATCTCCA ACCTGAAGGA GAGCACCACC 
TACACCATCT GCATGTCTGC AATGATCGGG GGACAGGAGG GAAGCCCCAC ACTCCTCACA 
GCCAAAACTT TGGATTTACC CAAAGTGACT GAGTTCATGG TGCAGGAGGC TGCGGAGACC 
AGCGTTTTGC TGACATGGAC AGCTGTTCCT GGGGCATCCA CTTATCTATT AACATGGCGC 
CTGTCCTCAG CTTCTGATCT TTCCCAGGAA CTGCTGTCAG CAGCTTCTAG ATCGTATCGA 
GTGACAGGAC TGAGCACCAA AGAAATGTAT GTGTTTTCCA TAAGACCAGT CTTTGGAGAT 
ACGGAGGGAC CAGAGACAAC ACTCCTTGGG CAAACAGCGG GACCTCACAG AGAGTCACCC 
ACACCCATCA CTCCAGTGAC TTCTACAAAA CTGGGTACGA TGAGATTCCC ATCCTTATCT 
CCTAGCAAGG TGGGGACCAC TCCTCTTCCA CTGGCCACCA CCACAACAGT ACCACCAACT 
CCAGCAACCC TACTGTCGAC CACTGCAGTG TCCACACTGC TGACAACTCT CCCTGGGCCA 
ATCTGTGGCA AGTTCAAAGC AGACATTGGG TTCCTGGTGG ACGAGTCCTC GAGTATTGGC 
CAGAGCAATT TCAACAAAGT GAAGGATTTC CTCTTCCGGA TCATTTCCTA TTTCCCGAAG 
ATTGGGCCTG AAGGGACACA GGTTGCTGTT GCTCAGTACA GCGAGGAGCC CAGGGCAGCC 
TTCTACTTCA ACCAGCACCG GGACCGCAAC AGTGCCCTGA AAGCCGTCAA GGGACTGAGC 
TACGCTGGGG GCAACACAAA AACAGGTCGT GGCATAACCT ACATGCTCAA GGAGTTATTT 
CAGTCCTCCA GAGGGATGAG GCCAGATTTC CCTCACGTGC TGGTGCTGGT GACCGATGGG 
CGATCCCAGG ATGATGTGTT GCTACCAGCC AGAGCAGCCC ATGAGTTGGG GATCCGCGTC 
ATTGCTGTGG GTGTCTCAGG AGCTGACCCC GTTGAGCTCA ACAGCATCTT GCTGCAGCAG 
AACCTCCAGA ATGTTTTCTA CGTCAGCACG TTTGATGACT TCCCCCAGAT CCTCCGAGAG 
CTGATAGAGG TCATCTGTTC TGACCCCGAG CCTTCAGGGC CTCAACTACC ATCTGGGGAG 
AGCTTCAGGA GTTCACCAGC TCCTCATGGA GGCTATGACC CATACAGCTT TACTACAAAG 
GGAGAGAAAG GAGAGAGGGG TCTACCTGGG AAGGATGGAA TTCCCGGGCT GCCGGGCAGA 
CCGGGGCGGA CAGGTCCTCC TGGTCCCCCT GGACTCATGG GTCTCCCTGG CATCCAAGGT 
GATACTGGCT CTCCTGGCTA TCCTGGACCA GCGGGGCCAA AAGGAGAGAG AGGCGAACCA 
GGCTATGTCC TGGGAGGAGT AGAGGTGATT CCTGGCCGAA ATGGGCAGCC TGGCCCCCCT 
GGACAGAAAG GGCAGCCGGG GGTTCCTGGG GTAGCAGGAC CACCAGGTTT ACCAGGGCCA 
CAGGGGCCAC CAGGCATCTC CATCAAGGGT GAACGTGGGG ATTCAGGTAC CAGGGGCCCT 
CGTGGGAGGA ATGGCCTCAA GGGTGACAGA GGAGGAATGG GAGAGCCGGG GAAACCTGGG 
TTGCCAGGCC CAGTAGGGCT AGATGGTGTT CCTGGATTGC CTGGACCAAG GGGAGAAAAG 
GGTGTGATGG GACCTCCAGG AGCACCAGGA CCAAAGGGTA ACAAAGGGCA AGGTGGTGTG 
AAAGGGGAAA AGGGACTCCC AGGACCTCCA GGGCAGAAGG GAGACCAGGG GAACCCAGGA 
TTTCCAGGTG CTCCAGCTAT GGGGGTTTTG GGACCTCCAG GCAAGAAAGG TGTCAGGGGT 
GATATTGGAC CAACAGGGGC CCTGGGACCA CAAGGAGACA AAGGAATACA AGGAGACAAA 
GGAGAGAAGG GAAGCCCAGG TTTCGGGATT CCTGGTCAAC CTGGGCTGAA GGGAGATCCA 
GGCGACAGGG GAAATGTAGG TTTGTCGGGC AAACCAGGTC AGAAGGGGGA TAGAGGTCTG 
AAGGGAGAGA AAGGTGAACC AGGACTACCT GGGAAACCAG GCGAGACCGG ACTAAGAGGT 
AAAGATGGAG AACCAGGGGA GAAAGGCACC TCTGGGACCA AGGGAGAAGC TGGTGTCCCT 
GGAGAACCAG GAGAGAGAGG AATCAGGGGG CCACCTGGAC TCCCAGGACG GCCTGGAGAG 
CAAGGGATAA AAGGCGATAC AGGACCACCT GGAAAGGATG GAATCACTGG AGAGAAGGGA 
GACAAGGGCG AAGCTGGTGA GCCTGGGCTG CCTGGGACTC CTGGAAGCCT TGTGTCTTCA 
CTGGAGAGCA CCATCACCCT GAAAGGCGAC AAGGGGGATC GTGGGAAGGA TGGCTTGAAA 
GGCGAAAGAG GTCCTCCAGG CCCAAAGGGA GAACCCGGTC CACCAGGCAA AGGAGTGGAA 
ATGAAGGATC TGGAGAGGCT TTTTGAAGCA AATGGTATCA AGCTGGCACT GCTGAAGGAC 
CTCTCCAATG CACTCCTGCA GGATGGGCTG GATGGGCTGG TGTGGCAGCT GGGTGGCTCC 
AGGACAAGCA AGACCTCCAG GAGAAAGCAA GCAGTTCTTC ATGCTACTGA GCATGGCGAT 
TCACTGGTGT TTGATACTTC TCCTGGTGCC CTGGCCAGCA CTGAGGTGAC AGAAGAAGCA 
CAGAGCCTGG AGGTGGAGGC TCAGTTTAGG GTGCCGGTGT CTGGAGGACT CCTTAAGGGT 
CCATCTGCTG GTGGTTCTGA ACCTGAGTAC CAGCCCCTAG GAAACCCTCC TGAGTCCTTG 
GAGAGAACAT CTGAGGAAGG AAAGGAGGAG AGAGACCCAA GATGGACAAT CACTTCTCTC 
AGTGGTAGCT TGGCTTTGCC CCCACTGCAC CCAGAAGATG ATCTGCAAGG AAACCAGTCC 
ATGGAGCTTC TTGAGTCCCT GGAGGGGAGA GTGGAAGGAC AACCACAGCA GGACACTAGC 
CACGAAAATG AGAATTTAGA CGACGGCTCT GCGCTCCCAA GGCACGTTAG AGCCCAGAGA 
TCTGCTGAGG ACCAGAGACA CGTGGTAAAG ACCCCATTTC ACCACGACAG CTTCGGGCTG 
GCCTTTGTGC AAGAGCAGAG CCATCCGTGT GACCCAGGAG GAAACGGCGA CCTTTCCTTG 
TTGGCTCCAT CACAGGTTCA TCCCCCTGGT GCTCCAGGAG CTCGGAAATC CCCATCTGGA 
TCCACGGCAT ACCCAGGCCC TCATCACCCA AGGAGAATTA AGAAGGAGGA AGCTGGTCCA 
CCAGATCCAC CATCCCATGG CCAAAAGGGA GAAATGGGAG CTCCGGGAGT CCGTGGAGAC 
AAAGGAGAAA AGGGGCAGCC TGGGGAGATG GGGGAACCTG GAGAAAAGGG CATCAAAGGA 
GACAGAGGTG AAAATGGTCA AAAGGGAGAA CCAGGAATTG GGTTCAGAGG CCCTGTTGGT 
CAGGCTGGAC CACCTGGATT TAAAGGTGAG CCAGGGGCTC CAGGGCCTCC AGGAGCTCAG 
GGAGTCCAGG GAATTCGTGG CAATGCCGGC ACCCCCGGGT CACAAGGGGA AAGAGGGGCT 
CCAGGTCTGC CTGGGATGCC AGGACAAAAG GGAGAGCGTG GGAAGAGAGG ACGGAATGGA 
TTTGCTGGGC CCTCAGGACC ATCAGGATCC CCAGGAAAAG AGGGGATTTC TGGGACACCT 
GGACCCAAAG GCAACAAGGG AGAAATCGGC GTTGGAGCAG CTGGCCCAAG AGGTCCTCGT 
GGACTGCCTG GCCCTCGGGG TGACGAGGGC ATCGTGGGAG TCCGTGGCCC CACCGGCATG 
ACGGGTCTAA AAGGCTTCCC GGGTGTGAAG GGCGAAAGAG GTGATCGGGG ACTTCTGGGA 
CCCAAAGGAG AAAGAGGTGA GCCCATGACT ATTTTTGGAC CCCAAGGCTA TAAAGGCAGT 
AAAGGAGATC CTGGTGAACG GGGCCTGCCA GGTTTTGACG GAGACAAAGG CGAGAAAGGA 
GAGGATGGGC CTATAGGAGA AAAGGGAGTC AAAGGTGAAG CTGGCTCCAA GGGAGTGATG 
GGGCTTTTTG GAACAAGAGG ACCAGTGGGA CAGAAGGGGG AGCCAGGAGA ACCAGGCTTG 
CCGGGCTCTG CTGGTGCAGC AGGTCTTAAT GGGAAGAACG GAAACAAGGG AGCCAAAGGT 
GACAGAGGTC TTCAGGGGCA GAAGGGGAAG CCGGGAGGAA AAGGTGATCC TGGGGCCACT 
GGTGACATTG GACAGAAAGG AACAAAGGGC TTACGAGGGC TTCCAGGACG CATCGGTGCT 
CCTGGAGCTG AAGGCATGAA GGGAGAAATT GGCAGTCCTG GAAAACCAGG AATCCCAGGA 
TCAGATGGAC CACACGGACA AAAGGGTGAA AAGGGAACAT CAGGAGACGA TGGTGCCTCG 
GGGATTCCTG GGGAGAAAGG AGAAAAGGGA GCCAAAGGAG TTCCTGGCTT GGGAGGCTTC 
AAAGGACAAA TGGGATGGCC TGGGAGGACT GGAGTTGCTG GACCAGATGG CCCTCAAGGG 
CCACAGGGTG AACCAGGGCC CCAGGGTCCA CGAGGGAAAA GAGGGAGACC TCAGCTCTGC 
CTCAGGGGAC CCCCAGGCAT GGATGGGAGA AAAGGAGAAA TGGGTGAAAA TGGTCCAGCC 
GGCCAGAAAG GAGAGAAGGG AGATCCTGGT CTTTCTGAGG AAGAGGTGAA GGAGGTGGTC 
AGGAGCGAAA TGAGAGGCAG ATGCGATTGT GGAGGCGAGT TGGGAAGGAC ACTCATGGAA 
AGGGGCCTGG AGGCAACTGA AATCAACAGG CAGCTCCAAG GGTTCCAAAA GTTCCAGCTT 
TGGGAACGCA CCAGAAGGGA TGGTGAAAGC CAGGAAGAAA TGCTGAACAA AACCCAGGGG 
ATGACCATGT TGATGACAAT GGTGAAGCCC GTCATGGAGC CACCTTCACT TATTGCAGCG 
GCTCCCCGGG CGCAGCAGAT AACAGGTCCC TCTCAGCGCA AGCGGCGAGA AGCCCACAGT 
CAATTACCAG CCCCCTGCCT GCAGCCGATG GATGAAGGCT CCTGCCAGCA CTATGCCCTG 
CTCTGGTATT ACCACCTGGA GGCAAACACC TGCCGGCCCT TCATCTTTGG GGGATGCCGA 
GGGAACAGCA ACCGCTTCGA AACCAAGTGG AAGTGTGAGC AGAAGTGCAA AACCTCAGCA 
GCCAGAGGGA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014816502.1
CDS	249..7763
Translation	MFCAQILALAVAVVQHRAVAVHEACRTAEVADILFLVGQSQGTGRESSWLVKDFISSMARSFENVVMGKGGIRLAVALYGEKPRMSIELTDYVTIEEMLVAIQDLSFKGGSLKTGSALAFAARAMSRLDTLREDAAKVVVLITDGKSSNSVEDEARVLQDLGVTVFAVGIKGADKNELNTIASEPTAEHVLYVEDFHLLHNVAPKLSRRLCFTASEPPRPIKQTVQAEKIVGPRDLYVSEHSHSTLRLTWMPATGKVTGYHVHLHPLLPSGQPVSEDQQQIVVDGDKSTVLVTDLKPNTKYVFTVRAIYADTVGESAVVKGKTTPVPPVTNFRVIEEGLFSLKVAWTPPLGKLEGYKIYIPRANRPGMTYEQVLRGDVSSHVLENLQEDREYSISIYAVYPQGPSQPVAAVGRTLKLLPVKSILLQNETTNTLQARWTPIRGATGYRLTWTSAEGSIQDVNLSNTYSYYMIQGLQPGTEYTVTINPIFGDVEGPVVSSKATTVASSTVQMLKVTEISTNSALVSWDSVAGATGYRVAWGPTPEFFGKDRPRQLALNRSITAYQLRNLAHDTEYVISLYVLFGSVEGPGITTSARTSPLGYVSNFKVMSYTSTSLSLVWSATAAATKFKVTWSPVGAEKEKQVAKTQYLGSRVLAYQIDQLLPNTLYKVSVRAVFNKTEGPEVTLTHRTAPLADSNPIQAVEDLRITDTGVNSLKLSWRRLPGVTHYKISWAPFNGGLETSQVVAAETVSFTISNLKESTTYTICMSAMIGGQEGSPTLLTAKTLDLPKVTEFMVQEAAETSVLLTWTAVPGASTYLLTWRLSSASDLSQELLSAASRSYRVTGLSTKEMYVFSIRPVFGDTEGPETTLLGQTAGPHRESPTPITPVTSTKLGTMRFPSLSPSKVGTTPLPLATTTTVPPTPATLLSTTAVSTLLTTLPGPICGKFKADIGFLVDESSSIGQSNFNKVKDFLFRIISYFPKIGPEGTQVAVAQYSEEPRAAFYFNQHRDRNSALKAVKGLSYAGGNTKTGRGITYMLKELFQSSRGMRPDFPHVLVLVTDGRSQDDVLLPARAAHELGIRVIAVGVSGADPVELNSILLQQNLQNVFYVSTFDDFPQILRELIEVICSDPEPSGPQLPSGESFRSSPAPHGGYDPYSFTTKGEKGERGLPGKDGIPGLPGRPGRTGPPGPPGLMGLPGIQGDTGSPGYPGPAGPKGERGEPGYVLGGVEVIPGRNGQPGPPGQKGQPGVPGVAGPPGLPGPQGPPGISIKGERGDSGTRGPRGRNGLKGDRGGMGEPGKPGLPGPVGLDGVPGLPGPRGEKGVMGPPGAPGPKGNKGQGGVKGEKGLPGPPGQKGDQGNPGFPGAPAMGVLGPPGKKGVRGDIGPTGALGPQGDKGIQGDKGEKGSPGFGIPGQPGLKGDPGDRGNVGLSGKPGQKGDRGLKGEKGEPGLPGKPGETGLRGKDGEPGEKGTSGTKGEAGVPGEPGERGIRGPPGLPGRPGEQGIKGDTGPPGKDGITGEKGDKGEAGEPGLPGTPGSLVSSLESTITLKGDKGDRGKDGLKGERGPPGPKGEPGPPGKGVEMKDLERLFEANGIKLALLKDLSNALLQDGLDGLVWQLGGSRTSKTSRRKQAVLHATEHGDSLVFDTSPGALASTEVTEEAQSLEVEAQFRVPVSGGLLKGPSAGGSEPEYQPLGNPPESLERTSEEGKEERDPRWTITSLSGSLALPPLHPEDDLQGNQSMELLESLEGRVEGQPQQDTSHENENLDDGSALPRHVRAQRSAEDQRHVVKTPFHHDSFGLAFVQEQSHPCDPGGNGDLSLLAPSQVHPPGAPGARKSPSGSTAYPGPHHPRRIKKEEAGPPDPPSHGQKGEMGAPGVRGDKGEKGQPGEMGEPGEKGIKGDRGENGQKGEPGIGFRGPVGQAGPPGFKGEPGAPGPPGAQGVQGIRGNAGTPGSQGERGAPGLPGMPGQKGERGKRGRNGFAGPSGPSGSPGKEGISGTPGPKGNKGEIGVGAAGPRGPRGLPGPRGDEGIVGVRGPTGMTGLKGFPGVKGERGDRGLLGPKGERGEPMTIFGPQGYKGSKGDPGERGLPGFDGDKGEKGEDGPIGEKGVKGEAGSKGVMGLFGTRGPVGQKGEPGEPGLPGSAGAAGLNGKNGNKGAKGDRGLQGQKGKPGGKGDPGATGDIGQKGTKGLRGLPGRIGAPGAEGMKGEIGSPGKPGIPGSDGPHGQKGEKGTSGDDGASGIPGEKGEKGAKGVPGLGGFKGQMGWPGRTGVAGPDGPQGPQGEPGPQGPRGKRGRPQLCLRGPPGMDGRKGEMGENGPAGQKGEKGDPGLSEEEVKEVVRSEMRGRCDCGGELGRTLMERGLEATEINRQLQGFQKFQLWERTRRDGESQEEMLNKTQGMTMLMTMVKPVMEPPSLIAAAPRAQQITGPSQRKRREAHSQLPAPCLQPMDEGSCQHYALLWYYHLEANTCRPFIFGGCRGNSNRFETKWKCEQKCKTSAARGR

Target ORF information:

RefSeq Version	XM_014961016.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax collagen alpha-1(VII) chain-like (LOC106899270), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014961016.1

ORF Insert Sequence:

ATGTTTTGTG CCCAGATCCT TGCCCTGGCA GTGGCTGTGG TGCAGCACAG AGCTGTGGCC 
GTTCATGAAG CATGCAGGAC GGCGGAGGTG GCCGACATTC TGTTCCTCGT GGGACAGTCA 
CAGGGCACAG GAAGGGAGAG CTCCTGGCTG GTCAAGGACT TCATCAGCAG CATGGCTCGT 
TCCTTTGAGA ACGTGGTGAT GGGCAAAGGA GGCATCCGGC TGGCAGTGGC ACTCTACGGG 
GAGAAACCAA GGATGAGTAT TGAGCTTACG GATTACGTGA CCATCGAAGA AATGTTGGTG 
GCAATTCAGG ATCTCTCCTT CAAAGGTGGA AGCTTAAAAA CAGGGTCAGC CTTGGCCTTT 
GCAGCTCGTG CCATGTCTCG TCTGGACACA CTGCGAGAGG ATGCAGCAAA GGTGGTTGTT 
TTGATCACGG ATGGAAAATC CAGCAACTCA GTTGAAGATG AAGCCCGGGT CCTGCAGGAT 
TTGGGGGTGA CGGTGTTTGC CGTAGGGATT AAGGGTGCTG ACAAGAATGA ATTGAACACA 
ATAGCTTCAG AGCCCACTGC AGAGCATGTG CTTTATGTTG AAGATTTCCA CCTCCTCCAT 
AACGTGGCCC CCAAACTCTC TCGAAGGCTC TGTTTCACTG CCTCGGAGCC ACCACGACCC 
ATCAAACAGA CTGTGCAAGC TGAGAAGATC GTCGGCCCCC GGGATTTGTA TGTCAGTGAG 
CACAGCCACA GCACGCTGAG ACTCACCTGG ATGCCAGCCA CGGGGAAGGT GACGGGCTAC 
CATGTCCATC TGCATCCCTT GCTGCCTTCA GGGCAGCCCG TTTCAGAAGA CCAGCAGCAG 
ATTGTGGTGG ACGGTGACAA AAGTACCGTG CTGGTGACTG ATCTGAAACC CAACACCAAG 
TATGTCTTCA CGGTGAGGGC CATCTATGCA GATACTGTTG GGGAGTCAGC GGTTGTCAAG 
GGGAAAACAA CACCTGTGCC TCCAGTCACT AATTTCCGTG TGATAGAAGA AGGACTTTTT 
AGCTTGAAGG TGGCTTGGAC ACCTCCGCTT GGCAAGCTGG AGGGCTACAA GATCTACATC 
CCCAGAGCCA ACAGACCAGG AATGACCTAC GAGCAGGTTC TGCGGGGTGA TGTCTCATCC 
CACGTGCTGG AGAACTTGCA GGAGGACAGA GAATACAGCA TCAGCATCTA CGCCGTGTAC 
CCCCAGGGCC CCAGCCAGCC TGTGGCTGCT GTGGGGAGAA CATTGAAGCT CCTGCCCGTG 
AAAAGCATCT TGCTGCAGAA TGAGACCACC AACACGCTCC AGGCAAGGTG GACACCCATC 
CGGGGAGCAA CGGGGTACAG GCTCACCTGG ACATCAGCAG AGGGCAGCAT CCAAGATGTG 
AACCTCAGCA ACACCTACAG CTACTACATG ATCCAGGGGC TGCAGCCTGG CACGGAGTAC 
ACTGTTACCA TCAACCCCAT CTTTGGGGAC GTCGAGGGAC CAGTGGTGAG CAGCAAAGCA 
ACGACGGTGG CATCGAGCAC CGTCCAGATG CTGAAAGTGA CTGAGATATC TACCAACAGT 
GCTCTGGTCT CCTGGGACTC AGTTGCTGGG GCCACTGGGT ATCGAGTGGC TTGGGGTCCT 
ACACCAGAGT TCTTTGGCAA AGACCGTCCT CGCCAGCTGG CCCTCAACAG GTCAATCACA 
GCCTACCAGC TCCGCAACCT GGCCCATGAC ACCGAGTACG TGATTTCCCT CTACGTGCTC 
TTTGGCTCCG TGGAAGGACC AGGGATCACA ACCTCAGCCA GGACATCTCC TCTTGGCTAC 
GTCTCCAATT TCAAGGTGAT GTCCTACACC AGCACAAGCC TGTCTCTGGT GTGGAGTGCC 
ACAGCAGCTG CCACCAAATT CAAAGTCACC TGGAGCCCTG TGGGAGCAGA AAAAGAAAAA 
CAGGTTGCCA AGACTCAGTA CCTGGGCAGT AGGGTGCTGG CTTACCAGAT TGACCAGTTA 
CTGCCCAACA CCCTGTATAA AGTCAGCGTT CGGGCTGTCT TCAACAAGAC TGAGGGACCA 
GAGGTGACTC TGACTCACCG CACAGCGCCT CTTGCAGACT CCAACCCCAT CCAGGCCGTT 
GAAGATCTGA GGATTACTGA CACCGGCGTA AATAGCTTGA AGCTGTCTTG GAGGAGACTT 
CCTGGGGTAA CTCATTATAA GATATCCTGG GCGCCGTTCA ATGGTGGCTT GGAAACCTCC 
CAGGTGGTTG CAGCAGAGAC GGTCTCCTTC ACGATCTCCA ACCTGAAGGA GAGCACCACC 
TACACCATCT GCATGTCTGC AATGATCGGG GGACAGGAGG GAAGCCCCAC ACTCCTCACA 
GCCAAAACTT TGGATTTACC CAAAGTGACT GAGTTCATGG TGCAGGAGGC TGCGGAGACC 
AGCGTTTTGC TGACATGGAC AGCTGTTCCT GGGGCATCCA CTTATCTATT AACATGGCGC 
CTGTCCTCAG CTTCTGATCT TTCCCAGGAA CTGCTGTCAG CAGCTTCTAG ATCGTATCGA 
GTGACAGGAC TGAGCACCAA AGAAATGTAT GTGTTTTCCA TAAGACCAGT CTTTGGAGAT 
ACGGAGGGAC CAGAGACAAC ACTCCTTGGG CAAACAGCGG GACCTCACAG AGAGTCACCC 
ACACCCATCA CTCCAGTGAC TTCTACAAAA CTGGGTACGA TGAGATTCCC ATCCTTATCT 
CCTAGCAAGG TGGGGACCAC TCCTCTTCCA CTGGCCACCA CCACAACAGT ACCACCAACT 
CCAGCAACCC TACTGTCGAC CACTGCAGTG TCCACACTGC TGACAACTCT CCCTGGGCCA 
ATCTGTGGCA AGTTCAAAGC AGACATTGGG TTCCTGGTGG ACGAGTCCTC GAGTATTGGC 
CAGAGCAATT TCAACAAAGT GAAGGATTTC CTCTTCCGGA TCATTTCCTA TTTCCCGAAG 
ATTGGGCCTG AAGGGACACA GGTTGCTGTT GCTCAGTACA GCGAGGAGCC CAGGGCAGCC 
TTCTACTTCA ACCAGCACCG GGACCGCAAC AGTGCCCTGA AAGCCGTCAA GGGACTGAGC 
TACGCTGGGG GCAACACAAA AACAGGTCGT GGCATAACCT ACATGCTCAA GGAGTTATTT 
CAGTCCTCCA GAGGGATGAG GCCAGATTTC CCTCACGTGC TGGTGCTGGT GACCGATGGG 
CGATCCCAGG ATGATGTGTT GCTACCAGCC AGAGCAGCCC ATGAGTTGGG GATCCGCGTC 
ATTGCTGTGG GTGTCTCAGG AGCTGACCCC GTTGAGCTCA ACAGCATCTT GCTGCAGCAG 
AACCTCCAGA ATGTTTTCTA CGTCAGCACG TTTGATGACT TCCCCCAGAT CCTCCGAGAG 
CTGATAGAGG TCATCTGTTC TGACCCCGAG CCTTCAGGGC CTCAACTACC ATCTGGGGAG 
AGCTTCAGGA GTTCACCAGC TCCTCATGGA GGCTATGACC CATACAGCTT TACTACAAAG 
GGAGAGAAAG GAGAGAGGGG TCTACCTGGG AAGGATGGAA TTCCCGGGCT GCCGGGCAGA 
CCGGGGCGGA CAGGTCCTCC TGGTCCCCCT GGACTCATGG GTCTCCCTGG CATCCAAGGT 
GATACTGGCT CTCCTGGCTA TCCTGGACCA GCGGGGCCAA AAGGAGAGAG AGGCGAACCA 
GGCTATGTCC TGGGAGGAGT AGAGGTGATT CCTGGCCGAA ATGGGCAGCC TGGCCCCCCT 
GGACAGAAAG GGCAGCCGGG GGTTCCTGGG GTAGCAGGAC CACCAGGTTT ACCAGGGCCA 
CAGGGGCCAC CAGGCATCTC CATCAAGGGT GAACGTGGGG ATTCAGGTAC CAGGGGCCCT 
CGTGGGAGGA ATGGCCTCAA GGGTGACAGA GGAGGAATGG GAGAGCCGGG GAAACCTGGG 
TTGCCAGGCC CAGTAGGGCT AGATGGTGTT CCTGGATTGC CTGGACCAAG GGGAGAAAAG 
GGTGTGATGG GACCTCCAGG AGCACCAGGA CCAAAGGGTA ACAAAGGGCA AGGTGGTGTG 
AAAGGGGAAA AGGGACTCCC AGGACCTCCA GGGCAGAAGG GAGACCAGGG GAACCCAGGA 
TTTCCAGGTG CTCCAGCTAT GGGGGTTTTG GGACCTCCAG GCAAGAAAGG TGTCAGGGGT 
GATATTGGAC CAACAGGGGC CCTGGGACCA CAAGGAGACA AAGGAATACA AGGAGACAAA 
GGAGAGAAGG GAAGCCCAGG TTTCGGGATT CCTGGTCAAC CTGGGCTGAA GGGAGATCCA 
GGCGACAGGG GAAATGTAGG TTTGTCGGGC AAACCAGGTC AGAAGGGGGA TAGAGGTCTG 
AAGGGAGAGA AAGGTGAACC AGGACTACCT GGGAAACCAG GCGAGACCGG ACTAAGAGGT 
AAAGATGGAG AACCAGGGGA GAAAGGCACC TCTGGGACCA AGGGAGAAGC TGGTGTCCCT 
GGAGAACCAG GAGAGAGAGG AATCAGGGGG CCACCTGGAC TCCCAGGACG GCCTGGAGAG 
CAAGGGATAA AAGGCGATAC AGGACCACCT GGAAAGGATG GAATCACTGG AGAGAAGGGA 
GACAAGGGCG AAGCTGGTGA GCCTGGGCTG CCTGGGACTC CTGGAAGCCT TGTGTCTTCA 
CTGGAGAGCA CCATCACCCT GAAAGGCGAC AAGGGGGATC GTGGGAAGGA TGGCTTGAAA 
GGCGAAAGAG GTCCTCCAGG CCCAAAGGGA GAACCCGGTC CACCAGGCAA AGGAGTGGAA 
ATGAAGGATC TGGAGAGGCT TTTTGAAGCA AATGGTATCA AGCTGGCACT GCTGAAGGAC 
CTCTCCAATG CACTCCTGCA GGATGGGCTG GATGGGCTGG TGTGGCAGCT GGGTGGCTCC 
AGGACAAGCA AGACCTCCAG GAGAAAGCAA GCAGTTCTTC ATGCTACTGA GCATGGCGAT 
TCACTGGTGT TTGATACTTC TCCTGGTGCC CTGGCCAGCA CTGAGGTGAC AGAAGAAGCA 
CAGAGCCTGG AGGTGGAGGC TCAGTTTAGG GTGCCGGTGT CTGGAGGACT CCTTAAGGGT 
CCATCTGCTG GTGGTTCTGA ACCTGAGTAC CAGCCCCTAG GAAACCCTCC TGAGTCCTTG 
GAGAGAACAT CTGAGGAAGG AAAGGAGGAG AGAGACCCAA GATGGACAAT CACTTCTCTC 
AGTGGTAGCT TGGCTTTGCC CCCACTGCAC CCAGAAGATG ATCTGCAAGG AAACCAGTCC 
ATGGAGCTTC TTGAGTCCCT GGAGGGGAGA GTGGAAGGAC AACCACAGCA GGACACTAGC 
CACGAAAATG AGAATTTAGA CGACGGCTCT GCGCTCCCAA GGCACGTTAG AGCCCAGAGA 
TCTGCTGAGG ACCAGAGACA CGTGGTAAAG ACCCCATTTC ACCACGACAG CTTCGGGCTG 
GCCTTTGTGC AAGAGCAGAG CCATCCGTGT GACCCAGGAG GAAACGGCGA CCTTTCCTTG 
TTGGCTCCAT CACAGGTTCA TCCCCCTGGT GCTCCAGGAG CTCGGAAATC CCCATCTGGA 
TCCACGGCAT ACCCAGGCCC TCATCACCCA AGGAGAATTA AGAAGGAGGA AGCTGGTCCA 
CCAGATCCAC CATCCCATGG CCAAAAGGGA GAAATGGGAG CTCCGGGAGT CCGTGGAGAC 
AAAGGAGAAA AGGGGCAGCC TGGGGAGATG GGGGAACCTG GAGAAAAGGG CATCAAAGGA 
GACAGAGGTG AAAATGGTCA AAAGGGAGAA CCAGGAATTG GGTTCAGAGG CCCTGTTGGT 
CAGGCTGGAC CACCTGGATT TAAAGGTGAG CCAGGGGCTC CAGGGCCTCC AGGAGCTCAG 
GGAGTCCAGG GAATTCGTGG CAATGCCGGC ACCCCCGGGT CACAAGGGGA AAGAGGGGCT 
CCAGGTCTGC CTGGGATGCC AGGACAAAAG GGAGAGCGTG GGAAGAGAGG ACGGAATGGA 
TTTGCTGGGC CCTCAGGACC ATCAGGATCC CCAGGAAAAG AGGGGATTTC TGGGACACCT 
GGACCCAAAG GCAACAAGGG AGAAATCGGC GTTGGAGCAG CTGGCCCAAG AGGTCCTCGT 
GGACTGCCTG GCCCTCGGGG TGACGAGGGC ATCGTGGGAG TCCGTGGCCC CACCGGCATG 
ACGGGTCTAA AAGGCTTCCC GGGTGTGAAG GGCGAAAGAG GTGATCGGGG ACTTCTGGGA 
CCCAAAGGAG AAAGAGGTGA GCCCATGACT ATTTTTGGAC CCCAAGGCTA TAAAGGCAGT 
AAAGGAGATC CTGGTGAACG GGGCCTGCCA GGTTTTGACG GAGACAAAGG CGAGAAAGGA 
GAGGATGGGC CTATAGGAGA AAAGGGAGTC AAAGGTGAAG CTGGCTCCAA GGGAGTGATG 
GGGCTTTTTG GAACAAGAGG ACCAGTGGGA CAGAAGGGGG AGCCAGGAGA ACCAGGCTTG 
CCGGGCTCTG CTGGTGCAGC AGGTCTTAAT GGGAAGAACG GAAACAAGGG AGCCAAAGGT 
GACAGAGGTC TTCAGGGGCA GAAGGGGAAG CCGGGAGGAA AAGGTGATCC TGGGGCCACT 
GGTGACATTG GACAGAAAGG AACAAAGGGC TTACGAGGGC TTCCAGGACG CATCGGTGCT 
CCTGGAGCTG AAGGCATGAA GGGAGAAATT GGCAGTCCTG GAAAACCAGG AATCCCAGGA 
TCAGATGGAC CACACGGACA AAAGGGTGAA AAGGGAACAT CAGGAGACGA TGGTGCCTCG 
GGGATTCCTG GGGAGAAAGG AGAAAAGGGA GCCAAAGGAG TTCCTGGCTT GGGAGGCTTC 
AAAGGACAAA TGGGATGGCC TGGGAGGACT GGAGTTGCTG GACCAGATGG CCCTCAAGGG 
CCACAGGGTG AACCAGGGCC CCAGGGTCCA CGAGGGAAAA GAGGGAGACC TCAGCTCTGC 
CTCAGGGGAC CCCCAGGCAT GGATGGGAGA AAAGGAGAAA TGGGTGAAAA TGGTCCAGCC 
GGCCAGAAAG GAGAGAAGGG AGATCCTGGT CTTTCTGAGG AAGAGGTGAA GGAGGTGGTC 
AGGAGCGAAA TGAGAGGCAG ATGCGATTGT GGAGGCGAGT TGGGAAGGAC ACTCATGGAA 
AGGGGCCTGG AGGCAACTGA AATCAACAGG CAGCTCCAAG GGTTCCAAAA GTTCCAGCTT 
TGGGAACGCA CCAGAAGGGA TGGTGAAAGC CAGGAAGAAA TGCTGAACAA AACCCAGGGG 
ATGACCATGT TGATGACAAT GGTGAAGCCC GTCATGGAGC CACCTTCACT TATTGCAGCG 
GCTCCCCGGG CGCAGCAGAT AACAGGTCCC TCTCAGCGCA AGCGGCGAGA AGCCCACAGT 
CAATTACCAG CCCCCTGCCT GCAGCCGATG GATGAAGGCT CCTGCCAGCA CTATGCCCTG 
CTCTGGTATT ACCACCTGGA GGCAAACACC TGCCGGCCCT TCATCTTTGG GGGATGCCGA 
GGGAACAGCA ACCGCTTCGA AACCAAGTGG AAGTGTGAGC AGAAGTGCAA AACCTCAGCA 
GCCAGAGGGA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106899270 ( XM_014961017.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014816503.1 Calidris pugnax collagen alpha-1(VII) chain-like (LOC106899270), transcript variant X3, mRNA.

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CloneID	OCa78934
Clone ID Related Accession (Same CDS sequence)	XM_014961016.1 , XM_014961017.1 , XM_014961015.1
Accession Version	XM_014961017.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	collagen alpha-1(VII) chain-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTGTG CCCAGATCCT TGCCCTGGCA GTGGCTGTGG TGCAGCACAG AGCTGTGGCC 
GTTCATGAAG CATGCAGGAC GGCGGAGGTG GCCGACATTC TGTTCCTCGT GGGACAGTCA 
CAGGGCACAG GAAGGGAGAG CTCCTGGCTG GTCAAGGACT TCATCAGCAG CATGGCTCGT 
TCCTTTGAGA ACGTGGTGAT GGGCAAAGGA GGCATCCGGC TGGCAGTGGC ACTCTACGGG 
GAGAAACCAA GGATGAGTAT TGAGCTTACG GATTACGTGA CCATCGAAGA AATGTTGGTG 
GCAATTCAGG ATCTCTCCTT CAAAGGTGGA AGCTTAAAAA CAGGGTCAGC CTTGGCCTTT 
GCAGCTCGTG CCATGTCTCG TCTGGACACA CTGCGAGAGG ATGCAGCAAA GGTGGTTGTT 
TTGATCACGG ATGGAAAATC CAGCAACTCA GTTGAAGATG AAGCCCGGGT CCTGCAGGAT 
TTGGGGGTGA CGGTGTTTGC CGTAGGGATT AAGGGTGCTG ACAAGAATGA ATTGAACACA 
ATAGCTTCAG AGCCCACTGC AGAGCATGTG CTTTATGTTG AAGATTTCCA CCTCCTCCAT 
AACGTGGCCC CCAAACTCTC TCGAAGGCTC TGTTTCACTG CCTCGGAGCC ACCACGACCC 
ATCAAACAGA CTGTGCAAGC TGAGAAGATC GTCGGCCCCC GGGATTTGTA TGTCAGTGAG 
CACAGCCACA GCACGCTGAG ACTCACCTGG ATGCCAGCCA CGGGGAAGGT GACGGGCTAC 
CATGTCCATC TGCATCCCTT GCTGCCTTCA GGGCAGCCCG TTTCAGAAGA CCAGCAGCAG 
ATTGTGGTGG ACGGTGACAA AAGTACCGTG CTGGTGACTG ATCTGAAACC CAACACCAAG 
TATGTCTTCA CGGTGAGGGC CATCTATGCA GATACTGTTG GGGAGTCAGC GGTTGTCAAG 
GGGAAAACAA CACCTGTGCC TCCAGTCACT AATTTCCGTG TGATAGAAGA AGGACTTTTT 
AGCTTGAAGG TGGCTTGGAC ACCTCCGCTT GGCAAGCTGG AGGGCTACAA GATCTACATC 
CCCAGAGCCA ACAGACCAGG AATGACCTAC GAGCAGGTTC TGCGGGGTGA TGTCTCATCC 
CACGTGCTGG AGAACTTGCA GGAGGACAGA GAATACAGCA TCAGCATCTA CGCCGTGTAC 
CCCCAGGGCC CCAGCCAGCC TGTGGCTGCT GTGGGGAGAA CATTGAAGCT CCTGCCCGTG 
AAAAGCATCT TGCTGCAGAA TGAGACCACC AACACGCTCC AGGCAAGGTG GACACCCATC 
CGGGGAGCAA CGGGGTACAG GCTCACCTGG ACATCAGCAG AGGGCAGCAT CCAAGATGTG 
AACCTCAGCA ACACCTACAG CTACTACATG ATCCAGGGGC TGCAGCCTGG CACGGAGTAC 
ACTGTTACCA TCAACCCCAT CTTTGGGGAC GTCGAGGGAC CAGTGGTGAG CAGCAAAGCA 
ACGACGGTGG CATCGAGCAC CGTCCAGATG CTGAAAGTGA CTGAGATATC TACCAACAGT 
GCTCTGGTCT CCTGGGACTC AGTTGCTGGG GCCACTGGGT ATCGAGTGGC TTGGGGTCCT 
ACACCAGAGT TCTTTGGCAA AGACCGTCCT CGCCAGCTGG CCCTCAACAG GTCAATCACA 
GCCTACCAGC TCCGCAACCT GGCCCATGAC ACCGAGTACG TGATTTCCCT CTACGTGCTC 
TTTGGCTCCG TGGAAGGACC AGGGATCACA ACCTCAGCCA GGACATCTCC TCTTGGCTAC 
GTCTCCAATT TCAAGGTGAT GTCCTACACC AGCACAAGCC TGTCTCTGGT GTGGAGTGCC 
ACAGCAGCTG CCACCAAATT CAAAGTCACC TGGAGCCCTG TGGGAGCAGA AAAAGAAAAA 
CAGGTTGCCA AGACTCAGTA CCTGGGCAGT AGGGTGCTGG CTTACCAGAT TGACCAGTTA 
CTGCCCAACA CCCTGTATAA AGTCAGCGTT CGGGCTGTCT TCAACAAGAC TGAGGGACCA 
GAGGTGACTC TGACTCACCG CACAGCGCCT CTTGCAGACT CCAACCCCAT CCAGGCCGTT 
GAAGATCTGA GGATTACTGA CACCGGCGTA AATAGCTTGA AGCTGTCTTG GAGGAGACTT 
CCTGGGGTAA CTCATTATAA GATATCCTGG GCGCCGTTCA ATGGTGGCTT GGAAACCTCC 
CAGGTGGTTG CAGCAGAGAC GGTCTCCTTC ACGATCTCCA ACCTGAAGGA GAGCACCACC 
TACACCATCT GCATGTCTGC AATGATCGGG GGACAGGAGG GAAGCCCCAC ACTCCTCACA 
GCCAAAACTT TGGATTTACC CAAAGTGACT GAGTTCATGG TGCAGGAGGC TGCGGAGACC 
AGCGTTTTGC TGACATGGAC AGCTGTTCCT GGGGCATCCA CTTATCTATT AACATGGCGC 
CTGTCCTCAG CTTCTGATCT TTCCCAGGAA CTGCTGTCAG CAGCTTCTAG ATCGTATCGA 
GTGACAGGAC TGAGCACCAA AGAAATGTAT GTGTTTTCCA TAAGACCAGT CTTTGGAGAT 
ACGGAGGGAC CAGAGACAAC ACTCCTTGGG CAAACAGCGG GACCTCACAG AGAGTCACCC 
ACACCCATCA CTCCAGTGAC TTCTACAAAA CTGGGTACGA TGAGATTCCC ATCCTTATCT 
CCTAGCAAGG TGGGGACCAC TCCTCTTCCA CTGGCCACCA CCACAACAGT ACCACCAACT 
CCAGCAACCC TACTGTCGAC CACTGCAGTG TCCACACTGC TGACAACTCT CCCTGGGCCA 
ATCTGTGGCA AGTTCAAAGC AGACATTGGG TTCCTGGTGG ACGAGTCCTC GAGTATTGGC 
CAGAGCAATT TCAACAAAGT GAAGGATTTC CTCTTCCGGA TCATTTCCTA TTTCCCGAAG 
ATTGGGCCTG AAGGGACACA GGTTGCTGTT GCTCAGTACA GCGAGGAGCC CAGGGCAGCC 
TTCTACTTCA ACCAGCACCG GGACCGCAAC AGTGCCCTGA AAGCCGTCAA GGGACTGAGC 
TACGCTGGGG GCAACACAAA AACAGGTCGT GGCATAACCT ACATGCTCAA GGAGTTATTT 
CAGTCCTCCA GAGGGATGAG GCCAGATTTC CCTCACGTGC TGGTGCTGGT GACCGATGGG 
CGATCCCAGG ATGATGTGTT GCTACCAGCC AGAGCAGCCC ATGAGTTGGG GATCCGCGTC 
ATTGCTGTGG GTGTCTCAGG AGCTGACCCC GTTGAGCTCA ACAGCATCTT GCTGCAGCAG 
AACCTCCAGA ATGTTTTCTA CGTCAGCACG TTTGATGACT TCCCCCAGAT CCTCCGAGAG 
CTGATAGAGG TCATCTGTTC TGACCCCGAG CCTTCAGGGC CTCAACTACC ATCTGGGGAG 
AGCTTCAGGA GTTCACCAGC TCCTCATGGA GGCTATGACC CATACAGCTT TACTACAAAG 
GGAGAGAAAG GAGAGAGGGG TCTACCTGGG AAGGATGGAA TTCCCGGGCT GCCGGGCAGA 
CCGGGGCGGA CAGGTCCTCC TGGTCCCCCT GGACTCATGG GTCTCCCTGG CATCCAAGGT 
GATACTGGCT CTCCTGGCTA TCCTGGACCA GCGGGGCCAA AAGGAGAGAG AGGCGAACCA 
GGCTATGTCC TGGGAGGAGT AGAGGTGATT CCTGGCCGAA ATGGGCAGCC TGGCCCCCCT 
GGACAGAAAG GGCAGCCGGG GGTTCCTGGG GTAGCAGGAC CACCAGGTTT ACCAGGGCCA 
CAGGGGCCAC CAGGCATCTC CATCAAGGGT GAACGTGGGG ATTCAGGTAC CAGGGGCCCT 
CGTGGGAGGA ATGGCCTCAA GGGTGACAGA GGAGGAATGG GAGAGCCGGG GAAACCTGGG 
TTGCCAGGCC CAGTAGGGCT AGATGGTGTT CCTGGATTGC CTGGACCAAG GGGAGAAAAG 
GGTGTGATGG GACCTCCAGG AGCACCAGGA CCAAAGGGTA ACAAAGGGCA AGGTGGTGTG 
AAAGGGGAAA AGGGACTCCC AGGACCTCCA GGGCAGAAGG GAGACCAGGG GAACCCAGGA 
TTTCCAGGTG CTCCAGCTAT GGGGGTTTTG GGACCTCCAG GCAAGAAAGG TGTCAGGGGT 
GATATTGGAC CAACAGGGGC CCTGGGACCA CAAGGAGACA AAGGAATACA AGGAGACAAA 
GGAGAGAAGG GAAGCCCAGG TTTCGGGATT CCTGGTCAAC CTGGGCTGAA GGGAGATCCA 
GGCGACAGGG GAAATGTAGG TTTGTCGGGC AAACCAGGTC AGAAGGGGGA TAGAGGTCTG 
AAGGGAGAGA AAGGTGAACC AGGACTACCT GGGAAACCAG GCGAGACCGG ACTAAGAGGT 
AAAGATGGAG AACCAGGGGA GAAAGGCACC TCTGGGACCA AGGGAGAAGC TGGTGTCCCT 
GGAGAACCAG GAGAGAGAGG AATCAGGGGG CCACCTGGAC TCCCAGGACG GCCTGGAGAG 
CAAGGGATAA AAGGCGATAC AGGACCACCT GGAAAGGATG GAATCACTGG AGAGAAGGGA 
GACAAGGGCG AAGCTGGTGA GCCTGGGCTG CCTGGGACTC CTGGAAGCCT TGTGTCTTCA 
CTGGAGAGCA CCATCACCCT GAAAGGCGAC AAGGGGGATC GTGGGAAGGA TGGCTTGAAA 
GGCGAAAGAG GTCCTCCAGG CCCAAAGGGA GAACCCGGTC CACCAGGCAA AGGAGTGGAA 
ATGAAGGATC TGGAGAGGCT TTTTGAAGCA AATGGTATCA AGCTGGCACT GCTGAAGGAC 
CTCTCCAATG CACTCCTGCA GGATGGGCTG GATGGGCTGG TGTGGCAGCT GGGTGGCTCC 
AGGACAAGCA AGACCTCCAG GAGAAAGCAA GCAGTTCTTC ATGCTACTGA GCATGGCGAT 
TCACTGGTGT TTGATACTTC TCCTGGTGCC CTGGCCAGCA CTGAGGTGAC AGAAGAAGCA 
CAGAGCCTGG AGGTGGAGGC TCAGTTTAGG GTGCCGGTGT CTGGAGGACT CCTTAAGGGT 
CCATCTGCTG GTGGTTCTGA ACCTGAGTAC CAGCCCCTAG GAAACCCTCC TGAGTCCTTG 
GAGAGAACAT CTGAGGAAGG AAAGGAGGAG AGAGACCCAA GATGGACAAT CACTTCTCTC 
AGTGGTAGCT TGGCTTTGCC CCCACTGCAC CCAGAAGATG ATCTGCAAGG AAACCAGTCC 
ATGGAGCTTC TTGAGTCCCT GGAGGGGAGA GTGGAAGGAC AACCACAGCA GGACACTAGC 
CACGAAAATG AGAATTTAGA CGACGGCTCT GCGCTCCCAA GGCACGTTAG AGCCCAGAGA 
TCTGCTGAGG ACCAGAGACA CGTGGTAAAG ACCCCATTTC ACCACGACAG CTTCGGGCTG 
GCCTTTGTGC AAGAGCAGAG CCATCCGTGT GACCCAGGAG GAAACGGCGA CCTTTCCTTG 
TTGGCTCCAT CACAGGTTCA TCCCCCTGGT GCTCCAGGAG CTCGGAAATC CCCATCTGGA 
TCCACGGCAT ACCCAGGCCC TCATCACCCA AGGAGAATTA AGAAGGAGGA AGCTGGTCCA 
CCAGATCCAC CATCCCATGG CCAAAAGGGA GAAATGGGAG CTCCGGGAGT CCGTGGAGAC 
AAAGGAGAAA AGGGGCAGCC TGGGGAGATG GGGGAACCTG GAGAAAAGGG CATCAAAGGA 
GACAGAGGTG AAAATGGTCA AAAGGGAGAA CCAGGAATTG GGTTCAGAGG CCCTGTTGGT 
CAGGCTGGAC CACCTGGATT TAAAGGTGAG CCAGGGGCTC CAGGGCCTCC AGGAGCTCAG 
GGAGTCCAGG GAATTCGTGG CAATGCCGGC ACCCCCGGGT CACAAGGGGA AAGAGGGGCT 
CCAGGTCTGC CTGGGATGCC AGGACAAAAG GGAGAGCGTG GGAAGAGAGG ACGGAATGGA 
TTTGCTGGGC CCTCAGGACC ATCAGGATCC CCAGGAAAAG AGGGGATTTC TGGGACACCT 
GGACCCAAAG GCAACAAGGG AGAAATCGGC GTTGGAGCAG CTGGCCCAAG AGGTCCTCGT 
GGACTGCCTG GCCCTCGGGG TGACGAGGGC ATCGTGGGAG TCCGTGGCCC CACCGGCATG 
ACGGGTCTAA AAGGCTTCCC GGGTGTGAAG GGCGAAAGAG GTGATCGGGG ACTTCTGGGA 
CCCAAAGGAG AAAGAGGTGA GCCCATGACT ATTTTTGGAC CCCAAGGCTA TAAAGGCAGT 
AAAGGAGATC CTGGTGAACG GGGCCTGCCA GGTTTTGACG GAGACAAAGG CGAGAAAGGA 
GAGGATGGGC CTATAGGAGA AAAGGGAGTC AAAGGTGAAG CTGGCTCCAA GGGAGTGATG 
GGGCTTTTTG GAACAAGAGG ACCAGTGGGA CAGAAGGGGG AGCCAGGAGA ACCAGGCTTG 
CCGGGCTCTG CTGGTGCAGC AGGTCTTAAT GGGAAGAACG GAAACAAGGG AGCCAAAGGT 
GACAGAGGTC TTCAGGGGCA GAAGGGGAAG CCGGGAGGAA AAGGTGATCC TGGGGCCACT 
GGTGACATTG GACAGAAAGG AACAAAGGGC TTACGAGGGC TTCCAGGACG CATCGGTGCT 
CCTGGAGCTG AAGGCATGAA GGGAGAAATT GGCAGTCCTG GAAAACCAGG AATCCCAGGA 
TCAGATGGAC CACACGGACA AAAGGGTGAA AAGGGAACAT CAGGAGACGA TGGTGCCTCG 
GGGATTCCTG GGGAGAAAGG AGAAAAGGGA GCCAAAGGAG TTCCTGGCTT GGGAGGCTTC 
AAAGGACAAA TGGGATGGCC TGGGAGGACT GGAGTTGCTG GACCAGATGG CCCTCAAGGG 
CCACAGGGTG AACCAGGGCC CCAGGGTCCA CGAGGGAAAA GAGGGAGACC TCAGCTCTGC 
CTCAGGGGAC CCCCAGGCAT GGATGGGAGA AAAGGAGAAA TGGGTGAAAA TGGTCCAGCC 
GGCCAGAAAG GAGAGAAGGG AGATCCTGGT CTTTCTGAGG AAGAGGTGAA GGAGGTGGTC 
AGGAGCGAAA TGAGAGGCAG ATGCGATTGT GGAGGCGAGT TGGGAAGGAC ACTCATGGAA 
AGGGGCCTGG AGGCAACTGA AATCAACAGG CAGCTCCAAG GGTTCCAAAA GTTCCAGCTT 
TGGGAACGCA CCAGAAGGGA TGGTGAAAGC CAGGAAGAAA TGCTGAACAA AACCCAGGGG 
ATGACCATGT TGATGACAAT GGTGAAGCCC GTCATGGAGC CACCTTCACT TATTGCAGCG 
GCTCCCCGGG CGCAGCAGAT AACAGGTCCC TCTCAGCGCA AGCGGCGAGA AGCCCACAGT 
CAATTACCAG CCCCCTGCCT GCAGCCGATG GATGAAGGCT CCTGCCAGCA CTATGCCCTG 
CTCTGGTATT ACCACCTGGA GGCAAACACC TGCCGGCCCT TCATCTTTGG GGGATGCCGA 
GGGAACAGCA ACCGCTTCGA AACCAAGTGG AAGTGTGAGC AGAAGTGCAA AACCTCAGCA 
GCCAGAGGGA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014816503.1
CDS	249..7763
Translation	MFCAQILALAVAVVQHRAVAVHEACRTAEVADILFLVGQSQGTGRESSWLVKDFISSMARSFENVVMGKGGIRLAVALYGEKPRMSIELTDYVTIEEMLVAIQDLSFKGGSLKTGSALAFAARAMSRLDTLREDAAKVVVLITDGKSSNSVEDEARVLQDLGVTVFAVGIKGADKNELNTIASEPTAEHVLYVEDFHLLHNVAPKLSRRLCFTASEPPRPIKQTVQAEKIVGPRDLYVSEHSHSTLRLTWMPATGKVTGYHVHLHPLLPSGQPVSEDQQQIVVDGDKSTVLVTDLKPNTKYVFTVRAIYADTVGESAVVKGKTTPVPPVTNFRVIEEGLFSLKVAWTPPLGKLEGYKIYIPRANRPGMTYEQVLRGDVSSHVLENLQEDREYSISIYAVYPQGPSQPVAAVGRTLKLLPVKSILLQNETTNTLQARWTPIRGATGYRLTWTSAEGSIQDVNLSNTYSYYMIQGLQPGTEYTVTINPIFGDVEGPVVSSKATTVASSTVQMLKVTEISTNSALVSWDSVAGATGYRVAWGPTPEFFGKDRPRQLALNRSITAYQLRNLAHDTEYVISLYVLFGSVEGPGITTSARTSPLGYVSNFKVMSYTSTSLSLVWSATAAATKFKVTWSPVGAEKEKQVAKTQYLGSRVLAYQIDQLLPNTLYKVSVRAVFNKTEGPEVTLTHRTAPLADSNPIQAVEDLRITDTGVNSLKLSWRRLPGVTHYKISWAPFNGGLETSQVVAAETVSFTISNLKESTTYTICMSAMIGGQEGSPTLLTAKTLDLPKVTEFMVQEAAETSVLLTWTAVPGASTYLLTWRLSSASDLSQELLSAASRSYRVTGLSTKEMYVFSIRPVFGDTEGPETTLLGQTAGPHRESPTPITPVTSTKLGTMRFPSLSPSKVGTTPLPLATTTTVPPTPATLLSTTAVSTLLTTLPGPICGKFKADIGFLVDESSSIGQSNFNKVKDFLFRIISYFPKIGPEGTQVAVAQYSEEPRAAFYFNQHRDRNSALKAVKGLSYAGGNTKTGRGITYMLKELFQSSRGMRPDFPHVLVLVTDGRSQDDVLLPARAAHELGIRVIAVGVSGADPVELNSILLQQNLQNVFYVSTFDDFPQILRELIEVICSDPEPSGPQLPSGESFRSSPAPHGGYDPYSFTTKGEKGERGLPGKDGIPGLPGRPGRTGPPGPPGLMGLPGIQGDTGSPGYPGPAGPKGERGEPGYVLGGVEVIPGRNGQPGPPGQKGQPGVPGVAGPPGLPGPQGPPGISIKGERGDSGTRGPRGRNGLKGDRGGMGEPGKPGLPGPVGLDGVPGLPGPRGEKGVMGPPGAPGPKGNKGQGGVKGEKGLPGPPGQKGDQGNPGFPGAPAMGVLGPPGKKGVRGDIGPTGALGPQGDKGIQGDKGEKGSPGFGIPGQPGLKGDPGDRGNVGLSGKPGQKGDRGLKGEKGEPGLPGKPGETGLRGKDGEPGEKGTSGTKGEAGVPGEPGERGIRGPPGLPGRPGEQGIKGDTGPPGKDGITGEKGDKGEAGEPGLPGTPGSLVSSLESTITLKGDKGDRGKDGLKGERGPPGPKGEPGPPGKGVEMKDLERLFEANGIKLALLKDLSNALLQDGLDGLVWQLGGSRTSKTSRRKQAVLHATEHGDSLVFDTSPGALASTEVTEEAQSLEVEAQFRVPVSGGLLKGPSAGGSEPEYQPLGNPPESLERTSEEGKEERDPRWTITSLSGSLALPPLHPEDDLQGNQSMELLESLEGRVEGQPQQDTSHENENLDDGSALPRHVRAQRSAEDQRHVVKTPFHHDSFGLAFVQEQSHPCDPGGNGDLSLLAPSQVHPPGAPGARKSPSGSTAYPGPHHPRRIKKEEAGPPDPPSHGQKGEMGAPGVRGDKGEKGQPGEMGEPGEKGIKGDRGENGQKGEPGIGFRGPVGQAGPPGFKGEPGAPGPPGAQGVQGIRGNAGTPGSQGERGAPGLPGMPGQKGERGKRGRNGFAGPSGPSGSPGKEGISGTPGPKGNKGEIGVGAAGPRGPRGLPGPRGDEGIVGVRGPTGMTGLKGFPGVKGERGDRGLLGPKGERGEPMTIFGPQGYKGSKGDPGERGLPGFDGDKGEKGEDGPIGEKGVKGEAGSKGVMGLFGTRGPVGQKGEPGEPGLPGSAGAAGLNGKNGNKGAKGDRGLQGQKGKPGGKGDPGATGDIGQKGTKGLRGLPGRIGAPGAEGMKGEIGSPGKPGIPGSDGPHGQKGEKGTSGDDGASGIPGEKGEKGAKGVPGLGGFKGQMGWPGRTGVAGPDGPQGPQGEPGPQGPRGKRGRPQLCLRGPPGMDGRKGEMGENGPAGQKGEKGDPGLSEEEVKEVVRSEMRGRCDCGGELGRTLMERGLEATEINRQLQGFQKFQLWERTRRDGESQEEMLNKTQGMTMLMTMVKPVMEPPSLIAAAPRAQQITGPSQRKRREAHSQLPAPCLQPMDEGSCQHYALLWYYHLEANTCRPFIFGGCRGNSNRFETKWKCEQKCKTSAARGR

Target ORF information:

RefSeq Version	XM_014961017.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax collagen alpha-1(VII) chain-like (LOC106899270), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014961017.1

ORF Insert Sequence:

ATGTTTTGTG CCCAGATCCT TGCCCTGGCA GTGGCTGTGG TGCAGCACAG AGCTGTGGCC 
GTTCATGAAG CATGCAGGAC GGCGGAGGTG GCCGACATTC TGTTCCTCGT GGGACAGTCA 
CAGGGCACAG GAAGGGAGAG CTCCTGGCTG GTCAAGGACT TCATCAGCAG CATGGCTCGT 
TCCTTTGAGA ACGTGGTGAT GGGCAAAGGA GGCATCCGGC TGGCAGTGGC ACTCTACGGG 
GAGAAACCAA GGATGAGTAT TGAGCTTACG GATTACGTGA CCATCGAAGA AATGTTGGTG 
GCAATTCAGG ATCTCTCCTT CAAAGGTGGA AGCTTAAAAA CAGGGTCAGC CTTGGCCTTT 
GCAGCTCGTG CCATGTCTCG TCTGGACACA CTGCGAGAGG ATGCAGCAAA GGTGGTTGTT 
TTGATCACGG ATGGAAAATC CAGCAACTCA GTTGAAGATG AAGCCCGGGT CCTGCAGGAT 
TTGGGGGTGA CGGTGTTTGC CGTAGGGATT AAGGGTGCTG ACAAGAATGA ATTGAACACA 
ATAGCTTCAG AGCCCACTGC AGAGCATGTG CTTTATGTTG AAGATTTCCA CCTCCTCCAT 
AACGTGGCCC CCAAACTCTC TCGAAGGCTC TGTTTCACTG CCTCGGAGCC ACCACGACCC 
ATCAAACAGA CTGTGCAAGC TGAGAAGATC GTCGGCCCCC GGGATTTGTA TGTCAGTGAG 
CACAGCCACA GCACGCTGAG ACTCACCTGG ATGCCAGCCA CGGGGAAGGT GACGGGCTAC 
CATGTCCATC TGCATCCCTT GCTGCCTTCA GGGCAGCCCG TTTCAGAAGA CCAGCAGCAG 
ATTGTGGTGG ACGGTGACAA AAGTACCGTG CTGGTGACTG ATCTGAAACC CAACACCAAG 
TATGTCTTCA CGGTGAGGGC CATCTATGCA GATACTGTTG GGGAGTCAGC GGTTGTCAAG 
GGGAAAACAA CACCTGTGCC TCCAGTCACT AATTTCCGTG TGATAGAAGA AGGACTTTTT 
AGCTTGAAGG TGGCTTGGAC ACCTCCGCTT GGCAAGCTGG AGGGCTACAA GATCTACATC 
CCCAGAGCCA ACAGACCAGG AATGACCTAC GAGCAGGTTC TGCGGGGTGA TGTCTCATCC 
CACGTGCTGG AGAACTTGCA GGAGGACAGA GAATACAGCA TCAGCATCTA CGCCGTGTAC 
CCCCAGGGCC CCAGCCAGCC TGTGGCTGCT GTGGGGAGAA CATTGAAGCT CCTGCCCGTG 
AAAAGCATCT TGCTGCAGAA TGAGACCACC AACACGCTCC AGGCAAGGTG GACACCCATC 
CGGGGAGCAA CGGGGTACAG GCTCACCTGG ACATCAGCAG AGGGCAGCAT CCAAGATGTG 
AACCTCAGCA ACACCTACAG CTACTACATG ATCCAGGGGC TGCAGCCTGG CACGGAGTAC 
ACTGTTACCA TCAACCCCAT CTTTGGGGAC GTCGAGGGAC CAGTGGTGAG CAGCAAAGCA 
ACGACGGTGG CATCGAGCAC CGTCCAGATG CTGAAAGTGA CTGAGATATC TACCAACAGT 
GCTCTGGTCT CCTGGGACTC AGTTGCTGGG GCCACTGGGT ATCGAGTGGC TTGGGGTCCT 
ACACCAGAGT TCTTTGGCAA AGACCGTCCT CGCCAGCTGG CCCTCAACAG GTCAATCACA 
GCCTACCAGC TCCGCAACCT GGCCCATGAC ACCGAGTACG TGATTTCCCT CTACGTGCTC 
TTTGGCTCCG TGGAAGGACC AGGGATCACA ACCTCAGCCA GGACATCTCC TCTTGGCTAC 
GTCTCCAATT TCAAGGTGAT GTCCTACACC AGCACAAGCC TGTCTCTGGT GTGGAGTGCC 
ACAGCAGCTG CCACCAAATT CAAAGTCACC TGGAGCCCTG TGGGAGCAGA AAAAGAAAAA 
CAGGTTGCCA AGACTCAGTA CCTGGGCAGT AGGGTGCTGG CTTACCAGAT TGACCAGTTA 
CTGCCCAACA CCCTGTATAA AGTCAGCGTT CGGGCTGTCT TCAACAAGAC TGAGGGACCA 
GAGGTGACTC TGACTCACCG CACAGCGCCT CTTGCAGACT CCAACCCCAT CCAGGCCGTT 
GAAGATCTGA GGATTACTGA CACCGGCGTA AATAGCTTGA AGCTGTCTTG GAGGAGACTT 
CCTGGGGTAA CTCATTATAA GATATCCTGG GCGCCGTTCA ATGGTGGCTT GGAAACCTCC 
CAGGTGGTTG CAGCAGAGAC GGTCTCCTTC ACGATCTCCA ACCTGAAGGA GAGCACCACC 
TACACCATCT GCATGTCTGC AATGATCGGG GGACAGGAGG GAAGCCCCAC ACTCCTCACA 
GCCAAAACTT TGGATTTACC CAAAGTGACT GAGTTCATGG TGCAGGAGGC TGCGGAGACC 
AGCGTTTTGC TGACATGGAC AGCTGTTCCT GGGGCATCCA CTTATCTATT AACATGGCGC 
CTGTCCTCAG CTTCTGATCT TTCCCAGGAA CTGCTGTCAG CAGCTTCTAG ATCGTATCGA 
GTGACAGGAC TGAGCACCAA AGAAATGTAT GTGTTTTCCA TAAGACCAGT CTTTGGAGAT 
ACGGAGGGAC CAGAGACAAC ACTCCTTGGG CAAACAGCGG GACCTCACAG AGAGTCACCC 
ACACCCATCA CTCCAGTGAC TTCTACAAAA CTGGGTACGA TGAGATTCCC ATCCTTATCT 
CCTAGCAAGG TGGGGACCAC TCCTCTTCCA CTGGCCACCA CCACAACAGT ACCACCAACT 
CCAGCAACCC TACTGTCGAC CACTGCAGTG TCCACACTGC TGACAACTCT CCCTGGGCCA 
ATCTGTGGCA AGTTCAAAGC AGACATTGGG TTCCTGGTGG ACGAGTCCTC GAGTATTGGC 
CAGAGCAATT TCAACAAAGT GAAGGATTTC CTCTTCCGGA TCATTTCCTA TTTCCCGAAG 
ATTGGGCCTG AAGGGACACA GGTTGCTGTT GCTCAGTACA GCGAGGAGCC CAGGGCAGCC 
TTCTACTTCA ACCAGCACCG GGACCGCAAC AGTGCCCTGA AAGCCGTCAA GGGACTGAGC 
TACGCTGGGG GCAACACAAA AACAGGTCGT GGCATAACCT ACATGCTCAA GGAGTTATTT 
CAGTCCTCCA GAGGGATGAG GCCAGATTTC CCTCACGTGC TGGTGCTGGT GACCGATGGG 
CGATCCCAGG ATGATGTGTT GCTACCAGCC AGAGCAGCCC ATGAGTTGGG GATCCGCGTC 
ATTGCTGTGG GTGTCTCAGG AGCTGACCCC GTTGAGCTCA ACAGCATCTT GCTGCAGCAG 
AACCTCCAGA ATGTTTTCTA CGTCAGCACG TTTGATGACT TCCCCCAGAT CCTCCGAGAG 
CTGATAGAGG TCATCTGTTC TGACCCCGAG CCTTCAGGGC CTCAACTACC ATCTGGGGAG 
AGCTTCAGGA GTTCACCAGC TCCTCATGGA GGCTATGACC CATACAGCTT TACTACAAAG 
GGAGAGAAAG GAGAGAGGGG TCTACCTGGG AAGGATGGAA TTCCCGGGCT GCCGGGCAGA 
CCGGGGCGGA CAGGTCCTCC TGGTCCCCCT GGACTCATGG GTCTCCCTGG CATCCAAGGT 
GATACTGGCT CTCCTGGCTA TCCTGGACCA GCGGGGCCAA AAGGAGAGAG AGGCGAACCA 
GGCTATGTCC TGGGAGGAGT AGAGGTGATT CCTGGCCGAA ATGGGCAGCC TGGCCCCCCT 
GGACAGAAAG GGCAGCCGGG GGTTCCTGGG GTAGCAGGAC CACCAGGTTT ACCAGGGCCA 
CAGGGGCCAC CAGGCATCTC CATCAAGGGT GAACGTGGGG ATTCAGGTAC CAGGGGCCCT 
CGTGGGAGGA ATGGCCTCAA GGGTGACAGA GGAGGAATGG GAGAGCCGGG GAAACCTGGG 
TTGCCAGGCC CAGTAGGGCT AGATGGTGTT CCTGGATTGC CTGGACCAAG GGGAGAAAAG 
GGTGTGATGG GACCTCCAGG AGCACCAGGA CCAAAGGGTA ACAAAGGGCA AGGTGGTGTG 
AAAGGGGAAA AGGGACTCCC AGGACCTCCA GGGCAGAAGG GAGACCAGGG GAACCCAGGA 
TTTCCAGGTG CTCCAGCTAT GGGGGTTTTG GGACCTCCAG GCAAGAAAGG TGTCAGGGGT 
GATATTGGAC CAACAGGGGC CCTGGGACCA CAAGGAGACA AAGGAATACA AGGAGACAAA 
GGAGAGAAGG GAAGCCCAGG TTTCGGGATT CCTGGTCAAC CTGGGCTGAA GGGAGATCCA 
GGCGACAGGG GAAATGTAGG TTTGTCGGGC AAACCAGGTC AGAAGGGGGA TAGAGGTCTG 
AAGGGAGAGA AAGGTGAACC AGGACTACCT GGGAAACCAG GCGAGACCGG ACTAAGAGGT 
AAAGATGGAG AACCAGGGGA GAAAGGCACC TCTGGGACCA AGGGAGAAGC TGGTGTCCCT 
GGAGAACCAG GAGAGAGAGG AATCAGGGGG CCACCTGGAC TCCCAGGACG GCCTGGAGAG 
CAAGGGATAA AAGGCGATAC AGGACCACCT GGAAAGGATG GAATCACTGG AGAGAAGGGA 
GACAAGGGCG AAGCTGGTGA GCCTGGGCTG CCTGGGACTC CTGGAAGCCT TGTGTCTTCA 
CTGGAGAGCA CCATCACCCT GAAAGGCGAC AAGGGGGATC GTGGGAAGGA TGGCTTGAAA 
GGCGAAAGAG GTCCTCCAGG CCCAAAGGGA GAACCCGGTC CACCAGGCAA AGGAGTGGAA 
ATGAAGGATC TGGAGAGGCT TTTTGAAGCA AATGGTATCA AGCTGGCACT GCTGAAGGAC 
CTCTCCAATG CACTCCTGCA GGATGGGCTG GATGGGCTGG TGTGGCAGCT GGGTGGCTCC 
AGGACAAGCA AGACCTCCAG GAGAAAGCAA GCAGTTCTTC ATGCTACTGA GCATGGCGAT 
TCACTGGTGT TTGATACTTC TCCTGGTGCC CTGGCCAGCA CTGAGGTGAC AGAAGAAGCA 
CAGAGCCTGG AGGTGGAGGC TCAGTTTAGG GTGCCGGTGT CTGGAGGACT CCTTAAGGGT 
CCATCTGCTG GTGGTTCTGA ACCTGAGTAC CAGCCCCTAG GAAACCCTCC TGAGTCCTTG 
GAGAGAACAT CTGAGGAAGG AAAGGAGGAG AGAGACCCAA GATGGACAAT CACTTCTCTC 
AGTGGTAGCT TGGCTTTGCC CCCACTGCAC CCAGAAGATG ATCTGCAAGG AAACCAGTCC 
ATGGAGCTTC TTGAGTCCCT GGAGGGGAGA GTGGAAGGAC AACCACAGCA GGACACTAGC 
CACGAAAATG AGAATTTAGA CGACGGCTCT GCGCTCCCAA GGCACGTTAG AGCCCAGAGA 
TCTGCTGAGG ACCAGAGACA CGTGGTAAAG ACCCCATTTC ACCACGACAG CTTCGGGCTG 
GCCTTTGTGC AAGAGCAGAG CCATCCGTGT GACCCAGGAG GAAACGGCGA CCTTTCCTTG 
TTGGCTCCAT CACAGGTTCA TCCCCCTGGT GCTCCAGGAG CTCGGAAATC CCCATCTGGA 
TCCACGGCAT ACCCAGGCCC TCATCACCCA AGGAGAATTA AGAAGGAGGA AGCTGGTCCA 
CCAGATCCAC CATCCCATGG CCAAAAGGGA GAAATGGGAG CTCCGGGAGT CCGTGGAGAC 
AAAGGAGAAA AGGGGCAGCC TGGGGAGATG GGGGAACCTG GAGAAAAGGG CATCAAAGGA 
GACAGAGGTG AAAATGGTCA AAAGGGAGAA CCAGGAATTG GGTTCAGAGG CCCTGTTGGT 
CAGGCTGGAC CACCTGGATT TAAAGGTGAG CCAGGGGCTC CAGGGCCTCC AGGAGCTCAG 
GGAGTCCAGG GAATTCGTGG CAATGCCGGC ACCCCCGGGT CACAAGGGGA AAGAGGGGCT 
CCAGGTCTGC CTGGGATGCC AGGACAAAAG GGAGAGCGTG GGAAGAGAGG ACGGAATGGA 
TTTGCTGGGC CCTCAGGACC ATCAGGATCC CCAGGAAAAG AGGGGATTTC TGGGACACCT 
GGACCCAAAG GCAACAAGGG AGAAATCGGC GTTGGAGCAG CTGGCCCAAG AGGTCCTCGT 
GGACTGCCTG GCCCTCGGGG TGACGAGGGC ATCGTGGGAG TCCGTGGCCC CACCGGCATG 
ACGGGTCTAA AAGGCTTCCC GGGTGTGAAG GGCGAAAGAG GTGATCGGGG ACTTCTGGGA 
CCCAAAGGAG AAAGAGGTGA GCCCATGACT ATTTTTGGAC CCCAAGGCTA TAAAGGCAGT 
AAAGGAGATC CTGGTGAACG GGGCCTGCCA GGTTTTGACG GAGACAAAGG CGAGAAAGGA 
GAGGATGGGC CTATAGGAGA AAAGGGAGTC AAAGGTGAAG CTGGCTCCAA GGGAGTGATG 
GGGCTTTTTG GAACAAGAGG ACCAGTGGGA CAGAAGGGGG AGCCAGGAGA ACCAGGCTTG 
CCGGGCTCTG CTGGTGCAGC AGGTCTTAAT GGGAAGAACG GAAACAAGGG AGCCAAAGGT 
GACAGAGGTC TTCAGGGGCA GAAGGGGAAG CCGGGAGGAA AAGGTGATCC TGGGGCCACT 
GGTGACATTG GACAGAAAGG AACAAAGGGC TTACGAGGGC TTCCAGGACG CATCGGTGCT 
CCTGGAGCTG AAGGCATGAA GGGAGAAATT GGCAGTCCTG GAAAACCAGG AATCCCAGGA 
TCAGATGGAC CACACGGACA AAAGGGTGAA AAGGGAACAT CAGGAGACGA TGGTGCCTCG 
GGGATTCCTG GGGAGAAAGG AGAAAAGGGA GCCAAAGGAG TTCCTGGCTT GGGAGGCTTC 
AAAGGACAAA TGGGATGGCC TGGGAGGACT GGAGTTGCTG GACCAGATGG CCCTCAAGGG 
CCACAGGGTG AACCAGGGCC CCAGGGTCCA CGAGGGAAAA GAGGGAGACC TCAGCTCTGC 
CTCAGGGGAC CCCCAGGCAT GGATGGGAGA AAAGGAGAAA TGGGTGAAAA TGGTCCAGCC 
GGCCAGAAAG GAGAGAAGGG AGATCCTGGT CTTTCTGAGG AAGAGGTGAA GGAGGTGGTC 
AGGAGCGAAA TGAGAGGCAG ATGCGATTGT GGAGGCGAGT TGGGAAGGAC ACTCATGGAA 
AGGGGCCTGG AGGCAACTGA AATCAACAGG CAGCTCCAAG GGTTCCAAAA GTTCCAGCTT 
TGGGAACGCA CCAGAAGGGA TGGTGAAAGC CAGGAAGAAA TGCTGAACAA AACCCAGGGG 
ATGACCATGT TGATGACAAT GGTGAAGCCC GTCATGGAGC CACCTTCACT TATTGCAGCG 
GCTCCCCGGG CGCAGCAGAT AACAGGTCCC TCTCAGCGCA AGCGGCGAGA AGCCCACAGT 
CAATTACCAG CCCCCTGCCT GCAGCCGATG GATGAAGGCT CCTGCCAGCA CTATGCCCTG 
CTCTGGTATT ACCACCTGGA GGCAAACACC TGCCGGCCCT TCATCTTTGG GGGATGCCGA 
GGGAACAGCA ACCGCTTCGA AACCAAGTGG AAGTGTGAGC AGAAGTGCAA AACCTCAGCA 
GCCAGAGGGA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106899270 ( XM_014961015.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014816501.1 Calidris pugnax collagen alpha-1(VII) chain-like (LOC106899270), transcript variant X1, mRNA.

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CloneID	OCa78934
Clone ID Related Accession (Same CDS sequence)	XM_014961016.1 , XM_014961017.1 , XM_014961015.1
Accession Version	XM_014961015.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	collagen alpha-1(VII) chain-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTGTG CCCAGATCCT TGCCCTGGCA GTGGCTGTGG TGCAGCACAG AGCTGTGGCC 
GTTCATGAAG CATGCAGGAC GGCGGAGGTG GCCGACATTC TGTTCCTCGT GGGACAGTCA 
CAGGGCACAG GAAGGGAGAG CTCCTGGCTG GTCAAGGACT TCATCAGCAG CATGGCTCGT 
TCCTTTGAGA ACGTGGTGAT GGGCAAAGGA GGCATCCGGC TGGCAGTGGC ACTCTACGGG 
GAGAAACCAA GGATGAGTAT TGAGCTTACG GATTACGTGA CCATCGAAGA AATGTTGGTG 
GCAATTCAGG ATCTCTCCTT CAAAGGTGGA AGCTTAAAAA CAGGGTCAGC CTTGGCCTTT 
GCAGCTCGTG CCATGTCTCG TCTGGACACA CTGCGAGAGG ATGCAGCAAA GGTGGTTGTT 
TTGATCACGG ATGGAAAATC CAGCAACTCA GTTGAAGATG AAGCCCGGGT CCTGCAGGAT 
TTGGGGGTGA CGGTGTTTGC CGTAGGGATT AAGGGTGCTG ACAAGAATGA ATTGAACACA 
ATAGCTTCAG AGCCCACTGC AGAGCATGTG CTTTATGTTG AAGATTTCCA CCTCCTCCAT 
AACGTGGCCC CCAAACTCTC TCGAAGGCTC TGTTTCACTG CCTCGGAGCC ACCACGACCC 
ATCAAACAGA CTGTGCAAGC TGAGAAGATC GTCGGCCCCC GGGATTTGTA TGTCAGTGAG 
CACAGCCACA GCACGCTGAG ACTCACCTGG ATGCCAGCCA CGGGGAAGGT GACGGGCTAC 
CATGTCCATC TGCATCCCTT GCTGCCTTCA GGGCAGCCCG TTTCAGAAGA CCAGCAGCAG 
ATTGTGGTGG ACGGTGACAA AAGTACCGTG CTGGTGACTG ATCTGAAACC CAACACCAAG 
TATGTCTTCA CGGTGAGGGC CATCTATGCA GATACTGTTG GGGAGTCAGC GGTTGTCAAG 
GGGAAAACAA CACCTGTGCC TCCAGTCACT AATTTCCGTG TGATAGAAGA AGGACTTTTT 
AGCTTGAAGG TGGCTTGGAC ACCTCCGCTT GGCAAGCTGG AGGGCTACAA GATCTACATC 
CCCAGAGCCA ACAGACCAGG AATGACCTAC GAGCAGGTTC TGCGGGGTGA TGTCTCATCC 
CACGTGCTGG AGAACTTGCA GGAGGACAGA GAATACAGCA TCAGCATCTA CGCCGTGTAC 
CCCCAGGGCC CCAGCCAGCC TGTGGCTGCT GTGGGGAGAA CATTGAAGCT CCTGCCCGTG 
AAAAGCATCT TGCTGCAGAA TGAGACCACC AACACGCTCC AGGCAAGGTG GACACCCATC 
CGGGGAGCAA CGGGGTACAG GCTCACCTGG ACATCAGCAG AGGGCAGCAT CCAAGATGTG 
AACCTCAGCA ACACCTACAG CTACTACATG ATCCAGGGGC TGCAGCCTGG CACGGAGTAC 
ACTGTTACCA TCAACCCCAT CTTTGGGGAC GTCGAGGGAC CAGTGGTGAG CAGCAAAGCA 
ACGACGGTGG CATCGAGCAC CGTCCAGATG CTGAAAGTGA CTGAGATATC TACCAACAGT 
GCTCTGGTCT CCTGGGACTC AGTTGCTGGG GCCACTGGGT ATCGAGTGGC TTGGGGTCCT 
ACACCAGAGT TCTTTGGCAA AGACCGTCCT CGCCAGCTGG CCCTCAACAG GTCAATCACA 
GCCTACCAGC TCCGCAACCT GGCCCATGAC ACCGAGTACG TGATTTCCCT CTACGTGCTC 
TTTGGCTCCG TGGAAGGACC AGGGATCACA ACCTCAGCCA GGACATCTCC TCTTGGCTAC 
GTCTCCAATT TCAAGGTGAT GTCCTACACC AGCACAAGCC TGTCTCTGGT GTGGAGTGCC 
ACAGCAGCTG CCACCAAATT CAAAGTCACC TGGAGCCCTG TGGGAGCAGA AAAAGAAAAA 
CAGGTTGCCA AGACTCAGTA CCTGGGCAGT AGGGTGCTGG CTTACCAGAT TGACCAGTTA 
CTGCCCAACA CCCTGTATAA AGTCAGCGTT CGGGCTGTCT TCAACAAGAC TGAGGGACCA 
GAGGTGACTC TGACTCACCG CACAGCGCCT CTTGCAGACT CCAACCCCAT CCAGGCCGTT 
GAAGATCTGA GGATTACTGA CACCGGCGTA AATAGCTTGA AGCTGTCTTG GAGGAGACTT 
CCTGGGGTAA CTCATTATAA GATATCCTGG GCGCCGTTCA ATGGTGGCTT GGAAACCTCC 
CAGGTGGTTG CAGCAGAGAC GGTCTCCTTC ACGATCTCCA ACCTGAAGGA GAGCACCACC 
TACACCATCT GCATGTCTGC AATGATCGGG GGACAGGAGG GAAGCCCCAC ACTCCTCACA 
GCCAAAACTT TGGATTTACC CAAAGTGACT GAGTTCATGG TGCAGGAGGC TGCGGAGACC 
AGCGTTTTGC TGACATGGAC AGCTGTTCCT GGGGCATCCA CTTATCTATT AACATGGCGC 
CTGTCCTCAG CTTCTGATCT TTCCCAGGAA CTGCTGTCAG CAGCTTCTAG ATCGTATCGA 
GTGACAGGAC TGAGCACCAA AGAAATGTAT GTGTTTTCCA TAAGACCAGT CTTTGGAGAT 
ACGGAGGGAC CAGAGACAAC ACTCCTTGGG CAAACAGCGG GACCTCACAG AGAGTCACCC 
ACACCCATCA CTCCAGTGAC TTCTACAAAA CTGGGTACGA TGAGATTCCC ATCCTTATCT 
CCTAGCAAGG TGGGGACCAC TCCTCTTCCA CTGGCCACCA CCACAACAGT ACCACCAACT 
CCAGCAACCC TACTGTCGAC CACTGCAGTG TCCACACTGC TGACAACTCT CCCTGGGCCA 
ATCTGTGGCA AGTTCAAAGC AGACATTGGG TTCCTGGTGG ACGAGTCCTC GAGTATTGGC 
CAGAGCAATT TCAACAAAGT GAAGGATTTC CTCTTCCGGA TCATTTCCTA TTTCCCGAAG 
ATTGGGCCTG AAGGGACACA GGTTGCTGTT GCTCAGTACA GCGAGGAGCC CAGGGCAGCC 
TTCTACTTCA ACCAGCACCG GGACCGCAAC AGTGCCCTGA AAGCCGTCAA GGGACTGAGC 
TACGCTGGGG GCAACACAAA AACAGGTCGT GGCATAACCT ACATGCTCAA GGAGTTATTT 
CAGTCCTCCA GAGGGATGAG GCCAGATTTC CCTCACGTGC TGGTGCTGGT GACCGATGGG 
CGATCCCAGG ATGATGTGTT GCTACCAGCC AGAGCAGCCC ATGAGTTGGG GATCCGCGTC 
ATTGCTGTGG GTGTCTCAGG AGCTGACCCC GTTGAGCTCA ACAGCATCTT GCTGCAGCAG 
AACCTCCAGA ATGTTTTCTA CGTCAGCACG TTTGATGACT TCCCCCAGAT CCTCCGAGAG 
CTGATAGAGG TCATCTGTTC TGACCCCGAG CCTTCAGGGC CTCAACTACC ATCTGGGGAG 
AGCTTCAGGA GTTCACCAGC TCCTCATGGA GGCTATGACC CATACAGCTT TACTACAAAG 
GGAGAGAAAG GAGAGAGGGG TCTACCTGGG AAGGATGGAA TTCCCGGGCT GCCGGGCAGA 
CCGGGGCGGA CAGGTCCTCC TGGTCCCCCT GGACTCATGG GTCTCCCTGG CATCCAAGGT 
GATACTGGCT CTCCTGGCTA TCCTGGACCA GCGGGGCCAA AAGGAGAGAG AGGCGAACCA 
GGCTATGTCC TGGGAGGAGT AGAGGTGATT CCTGGCCGAA ATGGGCAGCC TGGCCCCCCT 
GGACAGAAAG GGCAGCCGGG GGTTCCTGGG GTAGCAGGAC CACCAGGTTT ACCAGGGCCA 
CAGGGGCCAC CAGGCATCTC CATCAAGGGT GAACGTGGGG ATTCAGGTAC CAGGGGCCCT 
CGTGGGAGGA ATGGCCTCAA GGGTGACAGA GGAGGAATGG GAGAGCCGGG GAAACCTGGG 
TTGCCAGGCC CAGTAGGGCT AGATGGTGTT CCTGGATTGC CTGGACCAAG GGGAGAAAAG 
GGTGTGATGG GACCTCCAGG AGCACCAGGA CCAAAGGGTA ACAAAGGGCA AGGTGGTGTG 
AAAGGGGAAA AGGGACTCCC AGGACCTCCA GGGCAGAAGG GAGACCAGGG GAACCCAGGA 
TTTCCAGGTG CTCCAGCTAT GGGGGTTTTG GGACCTCCAG GCAAGAAAGG TGTCAGGGGT 
GATATTGGAC CAACAGGGGC CCTGGGACCA CAAGGAGACA AAGGAATACA AGGAGACAAA 
GGAGAGAAGG GAAGCCCAGG TTTCGGGATT CCTGGTCAAC CTGGGCTGAA GGGAGATCCA 
GGCGACAGGG GAAATGTAGG TTTGTCGGGC AAACCAGGTC AGAAGGGGGA TAGAGGTCTG 
AAGGGAGAGA AAGGTGAACC AGGACTACCT GGGAAACCAG GCGAGACCGG ACTAAGAGGT 
AAAGATGGAG AACCAGGGGA GAAAGGCACC TCTGGGACCA AGGGAGAAGC TGGTGTCCCT 
GGAGAACCAG GAGAGAGAGG AATCAGGGGG CCACCTGGAC TCCCAGGACG GCCTGGAGAG 
CAAGGGATAA AAGGCGATAC AGGACCACCT GGAAAGGATG GAATCACTGG AGAGAAGGGA 
GACAAGGGCG AAGCTGGTGA GCCTGGGCTG CCTGGGACTC CTGGAAGCCT TGTGTCTTCA 
CTGGAGAGCA CCATCACCCT GAAAGGCGAC AAGGGGGATC GTGGGAAGGA TGGCTTGAAA 
GGCGAAAGAG GTCCTCCAGG CCCAAAGGGA GAACCCGGTC CACCAGGCAA AGGAGTGGAA 
ATGAAGGATC TGGAGAGGCT TTTTGAAGCA AATGGTATCA AGCTGGCACT GCTGAAGGAC 
CTCTCCAATG CACTCCTGCA GGATGGGCTG GATGGGCTGG TGTGGCAGCT GGGTGGCTCC 
AGGACAAGCA AGACCTCCAG GAGAAAGCAA GCAGTTCTTC ATGCTACTGA GCATGGCGAT 
TCACTGGTGT TTGATACTTC TCCTGGTGCC CTGGCCAGCA CTGAGGTGAC AGAAGAAGCA 
CAGAGCCTGG AGGTGGAGGC TCAGTTTAGG GTGCCGGTGT CTGGAGGACT CCTTAAGGGT 
CCATCTGCTG GTGGTTCTGA ACCTGAGTAC CAGCCCCTAG GAAACCCTCC TGAGTCCTTG 
GAGAGAACAT CTGAGGAAGG AAAGGAGGAG AGAGACCCAA GATGGACAAT CACTTCTCTC 
AGTGGTAGCT TGGCTTTGCC CCCACTGCAC CCAGAAGATG ATCTGCAAGG AAACCAGTCC 
ATGGAGCTTC TTGAGTCCCT GGAGGGGAGA GTGGAAGGAC AACCACAGCA GGACACTAGC 
CACGAAAATG AGAATTTAGA CGACGGCTCT GCGCTCCCAA GGCACGTTAG AGCCCAGAGA 
TCTGCTGAGG ACCAGAGACA CGTGGTAAAG ACCCCATTTC ACCACGACAG CTTCGGGCTG 
GCCTTTGTGC AAGAGCAGAG CCATCCGTGT GACCCAGGAG GAAACGGCGA CCTTTCCTTG 
TTGGCTCCAT CACAGGTTCA TCCCCCTGGT GCTCCAGGAG CTCGGAAATC CCCATCTGGA 
TCCACGGCAT ACCCAGGCCC TCATCACCCA AGGAGAATTA AGAAGGAGGA AGCTGGTCCA 
CCAGATCCAC CATCCCATGG CCAAAAGGGA GAAATGGGAG CTCCGGGAGT CCGTGGAGAC 
AAAGGAGAAA AGGGGCAGCC TGGGGAGATG GGGGAACCTG GAGAAAAGGG CATCAAAGGA 
GACAGAGGTG AAAATGGTCA AAAGGGAGAA CCAGGAATTG GGTTCAGAGG CCCTGTTGGT 
CAGGCTGGAC CACCTGGATT TAAAGGTGAG CCAGGGGCTC CAGGGCCTCC AGGAGCTCAG 
GGAGTCCAGG GAATTCGTGG CAATGCCGGC ACCCCCGGGT CACAAGGGGA AAGAGGGGCT 
CCAGGTCTGC CTGGGATGCC AGGACAAAAG GGAGAGCGTG GGAAGAGAGG ACGGAATGGA 
TTTGCTGGGC CCTCAGGACC ATCAGGATCC CCAGGAAAAG AGGGGATTTC TGGGACACCT 
GGACCCAAAG GCAACAAGGG AGAAATCGGC GTTGGAGCAG CTGGCCCAAG AGGTCCTCGT 
GGACTGCCTG GCCCTCGGGG TGACGAGGGC ATCGTGGGAG TCCGTGGCCC CACCGGCATG 
ACGGGTCTAA AAGGCTTCCC GGGTGTGAAG GGCGAAAGAG GTGATCGGGG ACTTCTGGGA 
CCCAAAGGAG AAAGAGGTGA GCCCATGACT ATTTTTGGAC CCCAAGGCTA TAAAGGCAGT 
AAAGGAGATC CTGGTGAACG GGGCCTGCCA GGTTTTGACG GAGACAAAGG CGAGAAAGGA 
GAGGATGGGC CTATAGGAGA AAAGGGAGTC AAAGGTGAAG CTGGCTCCAA GGGAGTGATG 
GGGCTTTTTG GAACAAGAGG ACCAGTGGGA CAGAAGGGGG AGCCAGGAGA ACCAGGCTTG 
CCGGGCTCTG CTGGTGCAGC AGGTCTTAAT GGGAAGAACG GAAACAAGGG AGCCAAAGGT 
GACAGAGGTC TTCAGGGGCA GAAGGGGAAG CCGGGAGGAA AAGGTGATCC TGGGGCCACT 
GGTGACATTG GACAGAAAGG AACAAAGGGC TTACGAGGGC TTCCAGGACG CATCGGTGCT 
CCTGGAGCTG AAGGCATGAA GGGAGAAATT GGCAGTCCTG GAAAACCAGG AATCCCAGGA 
TCAGATGGAC CACACGGACA AAAGGGTGAA AAGGGAACAT CAGGAGACGA TGGTGCCTCG 
GGGATTCCTG GGGAGAAAGG AGAAAAGGGA GCCAAAGGAG TTCCTGGCTT GGGAGGCTTC 
AAAGGACAAA TGGGATGGCC TGGGAGGACT GGAGTTGCTG GACCAGATGG CCCTCAAGGG 
CCACAGGGTG AACCAGGGCC CCAGGGTCCA CGAGGGAAAA GAGGGAGACC TCAGCTCTGC 
CTCAGGGGAC CCCCAGGCAT GGATGGGAGA AAAGGAGAAA TGGGTGAAAA TGGTCCAGCC 
GGCCAGAAAG GAGAGAAGGG AGATCCTGGT CTTTCTGAGG AAGAGGTGAA GGAGGTGGTC 
AGGAGCGAAA TGAGAGGCAG ATGCGATTGT GGAGGCGAGT TGGGAAGGAC ACTCATGGAA 
AGGGGCCTGG AGGCAACTGA AATCAACAGG CAGCTCCAAG GGTTCCAAAA GTTCCAGCTT 
TGGGAACGCA CCAGAAGGGA TGGTGAAAGC CAGGAAGAAA TGCTGAACAA AACCCAGGGG 
ATGACCATGT TGATGACAAT GGTGAAGCCC GTCATGGAGC CACCTTCACT TATTGCAGCG 
GCTCCCCGGG CGCAGCAGAT AACAGGTCCC TCTCAGCGCA AGCGGCGAGA AGCCCACAGT 
CAATTACCAG CCCCCTGCCT GCAGCCGATG GATGAAGGCT CCTGCCAGCA CTATGCCCTG 
CTCTGGTATT ACCACCTGGA GGCAAACACC TGCCGGCCCT TCATCTTTGG GGGATGCCGA 
GGGAACAGCA ACCGCTTCGA AACCAAGTGG AAGTGTGAGC AGAAGTGCAA AACCTCAGCA 
GCCAGAGGGA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014816501.1
CDS	249..7763
Translation	MFCAQILALAVAVVQHRAVAVHEACRTAEVADILFLVGQSQGTGRESSWLVKDFISSMARSFENVVMGKGGIRLAVALYGEKPRMSIELTDYVTIEEMLVAIQDLSFKGGSLKTGSALAFAARAMSRLDTLREDAAKVVVLITDGKSSNSVEDEARVLQDLGVTVFAVGIKGADKNELNTIASEPTAEHVLYVEDFHLLHNVAPKLSRRLCFTASEPPRPIKQTVQAEKIVGPRDLYVSEHSHSTLRLTWMPATGKVTGYHVHLHPLLPSGQPVSEDQQQIVVDGDKSTVLVTDLKPNTKYVFTVRAIYADTVGESAVVKGKTTPVPPVTNFRVIEEGLFSLKVAWTPPLGKLEGYKIYIPRANRPGMTYEQVLRGDVSSHVLENLQEDREYSISIYAVYPQGPSQPVAAVGRTLKLLPVKSILLQNETTNTLQARWTPIRGATGYRLTWTSAEGSIQDVNLSNTYSYYMIQGLQPGTEYTVTINPIFGDVEGPVVSSKATTVASSTVQMLKVTEISTNSALVSWDSVAGATGYRVAWGPTPEFFGKDRPRQLALNRSITAYQLRNLAHDTEYVISLYVLFGSVEGPGITTSARTSPLGYVSNFKVMSYTSTSLSLVWSATAAATKFKVTWSPVGAEKEKQVAKTQYLGSRVLAYQIDQLLPNTLYKVSVRAVFNKTEGPEVTLTHRTAPLADSNPIQAVEDLRITDTGVNSLKLSWRRLPGVTHYKISWAPFNGGLETSQVVAAETVSFTISNLKESTTYTICMSAMIGGQEGSPTLLTAKTLDLPKVTEFMVQEAAETSVLLTWTAVPGASTYLLTWRLSSASDLSQELLSAASRSYRVTGLSTKEMYVFSIRPVFGDTEGPETTLLGQTAGPHRESPTPITPVTSTKLGTMRFPSLSPSKVGTTPLPLATTTTVPPTPATLLSTTAVSTLLTTLPGPICGKFKADIGFLVDESSSIGQSNFNKVKDFLFRIISYFPKIGPEGTQVAVAQYSEEPRAAFYFNQHRDRNSALKAVKGLSYAGGNTKTGRGITYMLKELFQSSRGMRPDFPHVLVLVTDGRSQDDVLLPARAAHELGIRVIAVGVSGADPVELNSILLQQNLQNVFYVSTFDDFPQILRELIEVICSDPEPSGPQLPSGESFRSSPAPHGGYDPYSFTTKGEKGERGLPGKDGIPGLPGRPGRTGPPGPPGLMGLPGIQGDTGSPGYPGPAGPKGERGEPGYVLGGVEVIPGRNGQPGPPGQKGQPGVPGVAGPPGLPGPQGPPGISIKGERGDSGTRGPRGRNGLKGDRGGMGEPGKPGLPGPVGLDGVPGLPGPRGEKGVMGPPGAPGPKGNKGQGGVKGEKGLPGPPGQKGDQGNPGFPGAPAMGVLGPPGKKGVRGDIGPTGALGPQGDKGIQGDKGEKGSPGFGIPGQPGLKGDPGDRGNVGLSGKPGQKGDRGLKGEKGEPGLPGKPGETGLRGKDGEPGEKGTSGTKGEAGVPGEPGERGIRGPPGLPGRPGEQGIKGDTGPPGKDGITGEKGDKGEAGEPGLPGTPGSLVSSLESTITLKGDKGDRGKDGLKGERGPPGPKGEPGPPGKGVEMKDLERLFEANGIKLALLKDLSNALLQDGLDGLVWQLGGSRTSKTSRRKQAVLHATEHGDSLVFDTSPGALASTEVTEEAQSLEVEAQFRVPVSGGLLKGPSAGGSEPEYQPLGNPPESLERTSEEGKEERDPRWTITSLSGSLALPPLHPEDDLQGNQSMELLESLEGRVEGQPQQDTSHENENLDDGSALPRHVRAQRSAEDQRHVVKTPFHHDSFGLAFVQEQSHPCDPGGNGDLSLLAPSQVHPPGAPGARKSPSGSTAYPGPHHPRRIKKEEAGPPDPPSHGQKGEMGAPGVRGDKGEKGQPGEMGEPGEKGIKGDRGENGQKGEPGIGFRGPVGQAGPPGFKGEPGAPGPPGAQGVQGIRGNAGTPGSQGERGAPGLPGMPGQKGERGKRGRNGFAGPSGPSGSPGKEGISGTPGPKGNKGEIGVGAAGPRGPRGLPGPRGDEGIVGVRGPTGMTGLKGFPGVKGERGDRGLLGPKGERGEPMTIFGPQGYKGSKGDPGERGLPGFDGDKGEKGEDGPIGEKGVKGEAGSKGVMGLFGTRGPVGQKGEPGEPGLPGSAGAAGLNGKNGNKGAKGDRGLQGQKGKPGGKGDPGATGDIGQKGTKGLRGLPGRIGAPGAEGMKGEIGSPGKPGIPGSDGPHGQKGEKGTSGDDGASGIPGEKGEKGAKGVPGLGGFKGQMGWPGRTGVAGPDGPQGPQGEPGPQGPRGKRGRPQLCLRGPPGMDGRKGEMGENGPAGQKGEKGDPGLSEEEVKEVVRSEMRGRCDCGGELGRTLMERGLEATEINRQLQGFQKFQLWERTRRDGESQEEMLNKTQGMTMLMTMVKPVMEPPSLIAAAPRAQQITGPSQRKRREAHSQLPAPCLQPMDEGSCQHYALLWYYHLEANTCRPFIFGGCRGNSNRFETKWKCEQKCKTSAARGR

Target ORF information:

RefSeq Version	XM_014961015.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax collagen alpha-1(VII) chain-like (LOC106899270), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014961015.1

ORF Insert Sequence:

ATGTTTTGTG CCCAGATCCT TGCCCTGGCA GTGGCTGTGG TGCAGCACAG AGCTGTGGCC 
GTTCATGAAG CATGCAGGAC GGCGGAGGTG GCCGACATTC TGTTCCTCGT GGGACAGTCA 
CAGGGCACAG GAAGGGAGAG CTCCTGGCTG GTCAAGGACT TCATCAGCAG CATGGCTCGT 
TCCTTTGAGA ACGTGGTGAT GGGCAAAGGA GGCATCCGGC TGGCAGTGGC ACTCTACGGG 
GAGAAACCAA GGATGAGTAT TGAGCTTACG GATTACGTGA CCATCGAAGA AATGTTGGTG 
GCAATTCAGG ATCTCTCCTT CAAAGGTGGA AGCTTAAAAA CAGGGTCAGC CTTGGCCTTT 
GCAGCTCGTG CCATGTCTCG TCTGGACACA CTGCGAGAGG ATGCAGCAAA GGTGGTTGTT 
TTGATCACGG ATGGAAAATC CAGCAACTCA GTTGAAGATG AAGCCCGGGT CCTGCAGGAT 
TTGGGGGTGA CGGTGTTTGC CGTAGGGATT AAGGGTGCTG ACAAGAATGA ATTGAACACA 
ATAGCTTCAG AGCCCACTGC AGAGCATGTG CTTTATGTTG AAGATTTCCA CCTCCTCCAT 
AACGTGGCCC CCAAACTCTC TCGAAGGCTC TGTTTCACTG CCTCGGAGCC ACCACGACCC 
ATCAAACAGA CTGTGCAAGC TGAGAAGATC GTCGGCCCCC GGGATTTGTA TGTCAGTGAG 
CACAGCCACA GCACGCTGAG ACTCACCTGG ATGCCAGCCA CGGGGAAGGT GACGGGCTAC 
CATGTCCATC TGCATCCCTT GCTGCCTTCA GGGCAGCCCG TTTCAGAAGA CCAGCAGCAG 
ATTGTGGTGG ACGGTGACAA AAGTACCGTG CTGGTGACTG ATCTGAAACC CAACACCAAG 
TATGTCTTCA CGGTGAGGGC CATCTATGCA GATACTGTTG GGGAGTCAGC GGTTGTCAAG 
GGGAAAACAA CACCTGTGCC TCCAGTCACT AATTTCCGTG TGATAGAAGA AGGACTTTTT 
AGCTTGAAGG TGGCTTGGAC ACCTCCGCTT GGCAAGCTGG AGGGCTACAA GATCTACATC 
CCCAGAGCCA ACAGACCAGG AATGACCTAC GAGCAGGTTC TGCGGGGTGA TGTCTCATCC 
CACGTGCTGG AGAACTTGCA GGAGGACAGA GAATACAGCA TCAGCATCTA CGCCGTGTAC 
CCCCAGGGCC CCAGCCAGCC TGTGGCTGCT GTGGGGAGAA CATTGAAGCT CCTGCCCGTG 
AAAAGCATCT TGCTGCAGAA TGAGACCACC AACACGCTCC AGGCAAGGTG GACACCCATC 
CGGGGAGCAA CGGGGTACAG GCTCACCTGG ACATCAGCAG AGGGCAGCAT CCAAGATGTG 
AACCTCAGCA ACACCTACAG CTACTACATG ATCCAGGGGC TGCAGCCTGG CACGGAGTAC 
ACTGTTACCA TCAACCCCAT CTTTGGGGAC GTCGAGGGAC CAGTGGTGAG CAGCAAAGCA 
ACGACGGTGG CATCGAGCAC CGTCCAGATG CTGAAAGTGA CTGAGATATC TACCAACAGT 
GCTCTGGTCT CCTGGGACTC AGTTGCTGGG GCCACTGGGT ATCGAGTGGC TTGGGGTCCT 
ACACCAGAGT TCTTTGGCAA AGACCGTCCT CGCCAGCTGG CCCTCAACAG GTCAATCACA 
GCCTACCAGC TCCGCAACCT GGCCCATGAC ACCGAGTACG TGATTTCCCT CTACGTGCTC 
TTTGGCTCCG TGGAAGGACC AGGGATCACA ACCTCAGCCA GGACATCTCC TCTTGGCTAC 
GTCTCCAATT TCAAGGTGAT GTCCTACACC AGCACAAGCC TGTCTCTGGT GTGGAGTGCC 
ACAGCAGCTG CCACCAAATT CAAAGTCACC TGGAGCCCTG TGGGAGCAGA AAAAGAAAAA 
CAGGTTGCCA AGACTCAGTA CCTGGGCAGT AGGGTGCTGG CTTACCAGAT TGACCAGTTA 
CTGCCCAACA CCCTGTATAA AGTCAGCGTT CGGGCTGTCT TCAACAAGAC TGAGGGACCA 
GAGGTGACTC TGACTCACCG CACAGCGCCT CTTGCAGACT CCAACCCCAT CCAGGCCGTT 
GAAGATCTGA GGATTACTGA CACCGGCGTA AATAGCTTGA AGCTGTCTTG GAGGAGACTT 
CCTGGGGTAA CTCATTATAA GATATCCTGG GCGCCGTTCA ATGGTGGCTT GGAAACCTCC 
CAGGTGGTTG CAGCAGAGAC GGTCTCCTTC ACGATCTCCA ACCTGAAGGA GAGCACCACC 
TACACCATCT GCATGTCTGC AATGATCGGG GGACAGGAGG GAAGCCCCAC ACTCCTCACA 
GCCAAAACTT TGGATTTACC CAAAGTGACT GAGTTCATGG TGCAGGAGGC TGCGGAGACC 
AGCGTTTTGC TGACATGGAC AGCTGTTCCT GGGGCATCCA CTTATCTATT AACATGGCGC 
CTGTCCTCAG CTTCTGATCT TTCCCAGGAA CTGCTGTCAG CAGCTTCTAG ATCGTATCGA 
GTGACAGGAC TGAGCACCAA AGAAATGTAT GTGTTTTCCA TAAGACCAGT CTTTGGAGAT 
ACGGAGGGAC CAGAGACAAC ACTCCTTGGG CAAACAGCGG GACCTCACAG AGAGTCACCC 
ACACCCATCA CTCCAGTGAC TTCTACAAAA CTGGGTACGA TGAGATTCCC ATCCTTATCT 
CCTAGCAAGG TGGGGACCAC TCCTCTTCCA CTGGCCACCA CCACAACAGT ACCACCAACT 
CCAGCAACCC TACTGTCGAC CACTGCAGTG TCCACACTGC TGACAACTCT CCCTGGGCCA 
ATCTGTGGCA AGTTCAAAGC AGACATTGGG TTCCTGGTGG ACGAGTCCTC GAGTATTGGC 
CAGAGCAATT TCAACAAAGT GAAGGATTTC CTCTTCCGGA TCATTTCCTA TTTCCCGAAG 
ATTGGGCCTG AAGGGACACA GGTTGCTGTT GCTCAGTACA GCGAGGAGCC CAGGGCAGCC 
TTCTACTTCA ACCAGCACCG GGACCGCAAC AGTGCCCTGA AAGCCGTCAA GGGACTGAGC 
TACGCTGGGG GCAACACAAA AACAGGTCGT GGCATAACCT ACATGCTCAA GGAGTTATTT 
CAGTCCTCCA GAGGGATGAG GCCAGATTTC CCTCACGTGC TGGTGCTGGT GACCGATGGG 
CGATCCCAGG ATGATGTGTT GCTACCAGCC AGAGCAGCCC ATGAGTTGGG GATCCGCGTC 
ATTGCTGTGG GTGTCTCAGG AGCTGACCCC GTTGAGCTCA ACAGCATCTT GCTGCAGCAG 
AACCTCCAGA ATGTTTTCTA CGTCAGCACG TTTGATGACT TCCCCCAGAT CCTCCGAGAG 
CTGATAGAGG TCATCTGTTC TGACCCCGAG CCTTCAGGGC CTCAACTACC ATCTGGGGAG 
AGCTTCAGGA GTTCACCAGC TCCTCATGGA GGCTATGACC CATACAGCTT TACTACAAAG 
GGAGAGAAAG GAGAGAGGGG TCTACCTGGG AAGGATGGAA TTCCCGGGCT GCCGGGCAGA 
CCGGGGCGGA CAGGTCCTCC TGGTCCCCCT GGACTCATGG GTCTCCCTGG CATCCAAGGT 
GATACTGGCT CTCCTGGCTA TCCTGGACCA GCGGGGCCAA AAGGAGAGAG AGGCGAACCA 
GGCTATGTCC TGGGAGGAGT AGAGGTGATT CCTGGCCGAA ATGGGCAGCC TGGCCCCCCT 
GGACAGAAAG GGCAGCCGGG GGTTCCTGGG GTAGCAGGAC CACCAGGTTT ACCAGGGCCA 
CAGGGGCCAC CAGGCATCTC CATCAAGGGT GAACGTGGGG ATTCAGGTAC CAGGGGCCCT 
CGTGGGAGGA ATGGCCTCAA GGGTGACAGA GGAGGAATGG GAGAGCCGGG GAAACCTGGG 
TTGCCAGGCC CAGTAGGGCT AGATGGTGTT CCTGGATTGC CTGGACCAAG GGGAGAAAAG 
GGTGTGATGG GACCTCCAGG AGCACCAGGA CCAAAGGGTA ACAAAGGGCA AGGTGGTGTG 
AAAGGGGAAA AGGGACTCCC AGGACCTCCA GGGCAGAAGG GAGACCAGGG GAACCCAGGA 
TTTCCAGGTG CTCCAGCTAT GGGGGTTTTG GGACCTCCAG GCAAGAAAGG TGTCAGGGGT 
GATATTGGAC CAACAGGGGC CCTGGGACCA CAAGGAGACA AAGGAATACA AGGAGACAAA 
GGAGAGAAGG GAAGCCCAGG TTTCGGGATT CCTGGTCAAC CTGGGCTGAA GGGAGATCCA 
GGCGACAGGG GAAATGTAGG TTTGTCGGGC AAACCAGGTC AGAAGGGGGA TAGAGGTCTG 
AAGGGAGAGA AAGGTGAACC AGGACTACCT GGGAAACCAG GCGAGACCGG ACTAAGAGGT 
AAAGATGGAG AACCAGGGGA GAAAGGCACC TCTGGGACCA AGGGAGAAGC TGGTGTCCCT 
GGAGAACCAG GAGAGAGAGG AATCAGGGGG CCACCTGGAC TCCCAGGACG GCCTGGAGAG 
CAAGGGATAA AAGGCGATAC AGGACCACCT GGAAAGGATG GAATCACTGG AGAGAAGGGA 
GACAAGGGCG AAGCTGGTGA GCCTGGGCTG CCTGGGACTC CTGGAAGCCT TGTGTCTTCA 
CTGGAGAGCA CCATCACCCT GAAAGGCGAC AAGGGGGATC GTGGGAAGGA TGGCTTGAAA 
GGCGAAAGAG GTCCTCCAGG CCCAAAGGGA GAACCCGGTC CACCAGGCAA AGGAGTGGAA 
ATGAAGGATC TGGAGAGGCT TTTTGAAGCA AATGGTATCA AGCTGGCACT GCTGAAGGAC 
CTCTCCAATG CACTCCTGCA GGATGGGCTG GATGGGCTGG TGTGGCAGCT GGGTGGCTCC 
AGGACAAGCA AGACCTCCAG GAGAAAGCAA GCAGTTCTTC ATGCTACTGA GCATGGCGAT 
TCACTGGTGT TTGATACTTC TCCTGGTGCC CTGGCCAGCA CTGAGGTGAC AGAAGAAGCA 
CAGAGCCTGG AGGTGGAGGC TCAGTTTAGG GTGCCGGTGT CTGGAGGACT CCTTAAGGGT 
CCATCTGCTG GTGGTTCTGA ACCTGAGTAC CAGCCCCTAG GAAACCCTCC TGAGTCCTTG 
GAGAGAACAT CTGAGGAAGG AAAGGAGGAG AGAGACCCAA GATGGACAAT CACTTCTCTC 
AGTGGTAGCT TGGCTTTGCC CCCACTGCAC CCAGAAGATG ATCTGCAAGG AAACCAGTCC 
ATGGAGCTTC TTGAGTCCCT GGAGGGGAGA GTGGAAGGAC AACCACAGCA GGACACTAGC 
CACGAAAATG AGAATTTAGA CGACGGCTCT GCGCTCCCAA GGCACGTTAG AGCCCAGAGA 
TCTGCTGAGG ACCAGAGACA CGTGGTAAAG ACCCCATTTC ACCACGACAG CTTCGGGCTG 
GCCTTTGTGC AAGAGCAGAG CCATCCGTGT GACCCAGGAG GAAACGGCGA CCTTTCCTTG 
TTGGCTCCAT CACAGGTTCA TCCCCCTGGT GCTCCAGGAG CTCGGAAATC CCCATCTGGA 
TCCACGGCAT ACCCAGGCCC TCATCACCCA AGGAGAATTA AGAAGGAGGA AGCTGGTCCA 
CCAGATCCAC CATCCCATGG CCAAAAGGGA GAAATGGGAG CTCCGGGAGT CCGTGGAGAC 
AAAGGAGAAA AGGGGCAGCC TGGGGAGATG GGGGAACCTG GAGAAAAGGG CATCAAAGGA 
GACAGAGGTG AAAATGGTCA AAAGGGAGAA CCAGGAATTG GGTTCAGAGG CCCTGTTGGT 
CAGGCTGGAC CACCTGGATT TAAAGGTGAG CCAGGGGCTC CAGGGCCTCC AGGAGCTCAG 
GGAGTCCAGG GAATTCGTGG CAATGCCGGC ACCCCCGGGT CACAAGGGGA AAGAGGGGCT 
CCAGGTCTGC CTGGGATGCC AGGACAAAAG GGAGAGCGTG GGAAGAGAGG ACGGAATGGA 
TTTGCTGGGC CCTCAGGACC ATCAGGATCC CCAGGAAAAG AGGGGATTTC TGGGACACCT 
GGACCCAAAG GCAACAAGGG AGAAATCGGC GTTGGAGCAG CTGGCCCAAG AGGTCCTCGT 
GGACTGCCTG GCCCTCGGGG TGACGAGGGC ATCGTGGGAG TCCGTGGCCC CACCGGCATG 
ACGGGTCTAA AAGGCTTCCC GGGTGTGAAG GGCGAAAGAG GTGATCGGGG ACTTCTGGGA 
CCCAAAGGAG AAAGAGGTGA GCCCATGACT ATTTTTGGAC CCCAAGGCTA TAAAGGCAGT 
AAAGGAGATC CTGGTGAACG GGGCCTGCCA GGTTTTGACG GAGACAAAGG CGAGAAAGGA 
GAGGATGGGC CTATAGGAGA AAAGGGAGTC AAAGGTGAAG CTGGCTCCAA GGGAGTGATG 
GGGCTTTTTG GAACAAGAGG ACCAGTGGGA CAGAAGGGGG AGCCAGGAGA ACCAGGCTTG 
CCGGGCTCTG CTGGTGCAGC AGGTCTTAAT GGGAAGAACG GAAACAAGGG AGCCAAAGGT 
GACAGAGGTC TTCAGGGGCA GAAGGGGAAG CCGGGAGGAA AAGGTGATCC TGGGGCCACT 
GGTGACATTG GACAGAAAGG AACAAAGGGC TTACGAGGGC TTCCAGGACG CATCGGTGCT 
CCTGGAGCTG AAGGCATGAA GGGAGAAATT GGCAGTCCTG GAAAACCAGG AATCCCAGGA 
TCAGATGGAC CACACGGACA AAAGGGTGAA AAGGGAACAT CAGGAGACGA TGGTGCCTCG 
GGGATTCCTG GGGAGAAAGG AGAAAAGGGA GCCAAAGGAG TTCCTGGCTT GGGAGGCTTC 
AAAGGACAAA TGGGATGGCC TGGGAGGACT GGAGTTGCTG GACCAGATGG CCCTCAAGGG 
CCACAGGGTG AACCAGGGCC CCAGGGTCCA CGAGGGAAAA GAGGGAGACC TCAGCTCTGC 
CTCAGGGGAC CCCCAGGCAT GGATGGGAGA AAAGGAGAAA TGGGTGAAAA TGGTCCAGCC 
GGCCAGAAAG GAGAGAAGGG AGATCCTGGT CTTTCTGAGG AAGAGGTGAA GGAGGTGGTC 
AGGAGCGAAA TGAGAGGCAG ATGCGATTGT GGAGGCGAGT TGGGAAGGAC ACTCATGGAA 
AGGGGCCTGG AGGCAACTGA AATCAACAGG CAGCTCCAAG GGTTCCAAAA GTTCCAGCTT 
TGGGAACGCA CCAGAAGGGA TGGTGAAAGC CAGGAAGAAA TGCTGAACAA AACCCAGGGG 
ATGACCATGT TGATGACAAT GGTGAAGCCC GTCATGGAGC CACCTTCACT TATTGCAGCG 
GCTCCCCGGG CGCAGCAGAT AACAGGTCCC TCTCAGCGCA AGCGGCGAGA AGCCCACAGT 
CAATTACCAG CCCCCTGCCT GCAGCCGATG GATGAAGGCT CCTGCCAGCA CTATGCCCTG 
CTCTGGTATT ACCACCTGGA GGCAAACACC TGCCGGCCCT TCATCTTTGG GGGATGCCGA 
GGGAACAGCA ACCGCTTCGA AACCAAGTGG AAGTGTGAGC AGAAGTGCAA AACCTCAGCA 
GCCAGAGGGA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106899270 ( XM_014961018.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014816504.1 Calidris pugnax collagen alpha-1(VII) chain-like (LOC106899270), transcript variant X4, mRNA.

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CloneID	OCa78935
Clone ID Related Accession (Same CDS sequence)	XM_014961018.1
Accession Version	XM_014961018.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	collagen alpha-1(VII) chain-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTGTG CCCAGATCCT TGCCCTGGCA GTGGCTGTGG TGCAGCACAG AGCTGTGGCC 
GTTCATGAAG CATGCAGGAC GGCGGAGGTG GCCGACATTC TGTTCCTCGT GGGACAGTCA 
CAGGGCACAG GAAGGGAGAG CTCCTGGCTG GTCAAGGACT TCATCAGCAG CATGGCTCGT 
TCCTTTGAGA ACGTGGTGAT GGGCAAAGGA GGCATCCGGC TGGCAGTGGC ACTCTACGGG 
GAGAAACCAA GGATGAGTAT TGAGCTTACG GATTACGTGA CCATCGAAGA AATGTTGGTG 
GCAATTCAGG ATCTCTCCTT CAAAGGTGGA AGCTTAAAAA CAGGGTCAGC CTTGGCCTTT 
GCAGCTCGTG CCATGTCTCG TCTGGACACA CTGCGAGAGG ATGCAGCAAA GGTGGTTGTT 
TTGATCACGG ATGGAAAATC CAGCAACTCA GTTGAAGATG AAGCCCGGGT CCTGCAGGAT 
TTGGGGGTGA CGGTGTTTGC CGTAGGGATT AAGGGTGCTG ACAAGAATGA ATTGAACACA 
ATAGCTTCAG AGCCCACTGC AGAGCATGTG CTTTATGTTG AAGATTTCCA CCTCCTCCAT 
AACGTGGCCC CCAAACTCTC TCGAAGGCTC TGTTTCACTG CCTCGGAGCC ACCACGACCC 
ATCAAACAGA CTGTGCAAGC TGAGAAGATC GTCGGCCCCC GGGATTTGTA TGTCAGTGAG 
CACAGCCACA GCACGCTGAG ACTCACCTGG ATGCCAGCCA CGGGGAAGGT GACGGGCTAC 
CATGTCCATC TGCATCCCTT GCTGCCTTCA GGGCAGCCCG TTTCAGAAGA CCAGCAGCAG 
ATTGTGGTGG ACGGTGACAA AAGTACCGTG CTGGTGACTG ATCTGAAACC CAACACCAAG 
TATGTCTTCA CGGTGAGGGC CATCTATGCA GATACTGTTG GGGAGTCAGC GGTTGTCAAG 
GGGAAAACAA CACCTGTGCC TCCAGTCACT AATTTCCGTG TGATAGAAGA AGGACTTTTT 
AGCTTGAAGG TGGCTTGGAC ACCTCCGCTT GGCAAGCTGG AGGGCTACAA GATCTACATC 
CCCAGAGCCA ACAGACCAGG AATGACCTAC GAGCAGGTTC TGCGGGGTGA TGTCTCATCC 
CACGTGCTGG AGAACTTGCA GGAGGACAGA GAATACAGCA TCAGCATCTA CGCCGTGTAC 
CCCCAGGGCC CCAGCCAGCC TGTGGCTGCT GTGGGGAGAA CATTGAAGCT CCTGCCCGTG 
AAAAGCATCT TGCTGCAGAA TGAGACCACC AACACGCTCC AGGCAAGGTG GACACCCATC 
CGGGGAGCAA CGGGGTACAG GCTCACCTGG ACATCAGCAG AGGGCAGCAT CCAAGATGTG 
AACCTCAGCA ACACCTACAG CTACTACATG ATCCAGGGGC TGCAGCCTGG CACGGAGTAC 
ACTGTTACCA TCAACCCCAT CTTTGGGGAC GTCGAGGGAC CAGTGGTGAG CAGCAAAGCA 
ACGACGGTGG CATCGAGCAC CGTCCAGATG CTGAAAGTGA CTGAGATATC TACCAACAGT 
GCTCTGGTCT CCTGGGACTC AGTTGCTGGG GCCACTGGGT ATCGAGTGGC TTGGGGTCCT 
ACACCAGAGT TCTTTGGCAA AGACCGTCCT CGCCAGCTGG CCCTCAACAG GTCAATCACA 
GCCTACCAGC TCCGCAACCT GGCCCATGAC ACCGAGTACG TGATTTCCCT CTACGTGCTC 
TTTGGCTCCG TGGAAGGACC AGGGATCACA ACCTCAGCCA GGACATCTCC TCTTGGCTAC 
GTCTCCAATT TCAAGGTGAT GTCCTACACC AGCACAAGCC TGTCTCTGGT GTGGAGTGCC 
ACAGCAGCTG CCACCAAATT CAAAGTCACC TGGAGCCCTG TGGGAGCAGA AAAAGAAAAA 
CAGGTTGCCA AGACTCAGTA CCTGGGCAGT AGGGTGCTGG CTTACCAGAT TGACCAGTTA 
CTGCCCAACA CCCTGTATAA AGTCAGCGTT CGGGCTGTCT TCAACAAGAC TGAGGGACCA 
GAGGTGACTC TGACTCACCG CACAGCGCCT CTTGCAGACT CCAACCCCAT CCAGGCCGTT 
GAAGATCTGA GGATTACTGA CACCGGCGTA AATAGCTTGA AGCTGTCTTG GAGGAGACTT 
CCTGGGGTAA CTCATTATAA GATATCCTGG GCGCCGTTCA ATGGTGGCTT GGAAACCTCC 
CAGGTGGTTG CAGCAGAGAC GGTCTCCTTC ACGATCTCCA ACCTGAAGGA GAGCACCACC 
TACACCATCT GCATGTCTGC AATGATCGGG GGACAGGAGG GAAGCCCCAC ACTCCTCACA 
GCCAAAACTT TGGATTTACC CAAAGTGACT GAGTTCATGG TGCAGGAGGC TGCGGAGACC 
AGCGTTTTGC TGACATGGAC AGCTGTTCCT GGGGCATCCA CTTATCTATT AACATGGCGC 
CTGTCCTCAG CTTCTGATCT TTCCCAGGAA CTGCTGTCAG CAGCTTCTAG ATCGTATCGA 
GTGACAGGAC TGAGCACCAA AGAAATGTAT GTGTTTTCCA TAAGACCAGT CTTTGGAGAT 
ACGGAGGGAC CAGAGACAAC ACTCCTTGGG CAAACAGCGG GACCTCACAG AGAGTCACCC 
ACACCCATCA CTCCAGTGAC TTCTACAAAA CTGGGTACGA TGAGATTCCC ATCCTTATCT 
CCTAGCAAGG TGGGGACCAC TCCTCTTCCA CTGGCCACCA CCACAACAGT ACCACCAACT 
CCAGCAACCC TACTGTCGAC CACTGCAGTG TCCACACTGC TGACAACTCT CCCTGGGCCA 
ATCTGTGGCA AGTTCAAAGC AGACATTGGG TTCCTGGTGG ACGAGTCCTC GAGTATTGGC 
CAGAGCAATT TCAACAAAGT GAAGGATTTC CTCTTCCGGA TCATTTCCTA TTTCCCGAAG 
ATTGGGCCTG AAGGGACACA GGTTGCTGTT GCTCAGTACA GCGAGGAGCC CAGGGCAGCC 
TTCTACTTCA ACCAGCACCG GGACCGCAAC AGTGCCCTGA AAGCCGTCAA GGGACTGAGC 
TACGCTGGGG GCAACACAAA AACAGGTCGT GGCATAACCT ACATGCTCAA GGAGTTATTT 
CAGTCCTCCA GAGGGATGAG GCCAGATTTC CCTCACGTGC TGGTGCTGGT GACCGATGGG 
CGATCCCAGG ATGATGTGTT GCTACCAGCC AGAGCAGCCC ATGAGTTGGG GATCCGCGTC 
ATTGCTGTGG GTGTCTCAGG AGCTGACCCC GTTGAGCTCA ACAGCATCTT GCTGCAGCAG 
AACCTCCAGA ATGTTTTCTA CGTCAGCACG TTTGATGACT TCCCCCAGAT CCTCCGAGAG 
CTGATAGAGG TCATCTGTTC TGACCCCGAG CCTTCAGGGC CTCAACTACC ATCTGGGGAG 
AGCTTCAGGA GTTCACCAGC TCCTCATGGA GGCTATGACC CATACAGCTT TACTACAAAG 
GGAGAGAAAG GAGAGAGGGG TCTACCTGGG AAGGATGGAA TTCCCGGGCT GCCGGGCAGA 
CCGGGGCGGA CAGGTCCTCC TGGTCCCCCT GGACTCATGG GTCTCCCTGG CATCCAAGGT 
GATACTGGCT CTCCTGGCTA TCCTGGACCA GCGGGGCCAA AAGGAGAGAG AGGCGAACCA 
GGCTATGTCC TGGGAGGAGT AGAGGTGATT CCTGGCCGAA ATGGGCAGCC TGGCCCCCCT 
GGACAGAAAG GGCAGCCGGG GGTTCCTGGG GTAGCAGGAC CACCAGGTTT ACCAGGGCCA 
CAGGGGCCAC CAGGCATCTC CATCAAGGGT GAACGTGGGG ATTCAGGTAC CAGGGGCCCT 
CGTGGGAGGA ATGGCCTCAA GGGTGACAGA GGAGGAATGG GAGAGCCGGG GAAACCTGGG 
TTGCCAGGCC CAGTAGGGCT AGATGGTGTT CCTGGATTGC CTGGACCAAG GGGAGAAAAG 
GGTGTGATGG GACCTCCAGG AGCACCAGGA CCAAAGGGTA ACAAAGGGCA AGGTGGTGTG 
AAAGGGGAAA AGGGACTCCC AGGACCTCCA GGGCAGAAGG GAGACCAGGG GAACCCAGGA 
TTTCCAGGTG CTCCAGCTAT GGGGGTTTTG GGACCTCCAG GCAAGAAAGG TGTCAGGGGT 
GATATTGGAC CAACAGGGGC CCTGGGACCA CAAGGAGACA AAGGAATACA AGGAGACAAA 
GGAGAGAAGG GAAGCCCAGG TTTCGGGATT CCTGGTCAAC CTGGGCTGAA GGGAGATCCA 
GGCGACAGGG GAAATGTAGG TTTGTCGGGC AAACCAGGTC AGAAGGGGGA TAGAGGTCTG 
AAGGGAGAGA AAGGTGAACC AGGACTACCT GGGAAACCAG GCGAGACCGG ACTAAGAGGT 
AAAGATGGAG AAGCTGGTGT CCCTGGAGAA CCAGGAGAGA GAGGAATCAG GGGGCCACCT 
GGACTCCCAG GACGGCCTGG AGAGCAAGGG ATAAAAGGCG ATACAGGACC ACCTGGAAAG 
GATGGAATCA CTGGAGAGAA GGGAGACAAG GGCGAAGCTG GTGAGCCTGG GCTGCCTGGG 
ACTCCTGGAA GCCTTGTGTC TTCACTGGAG AGCACCATCA CCCTGAAAGG CGACAAGGGG 
GATCGTGGGA AGGATGGCTT GAAAGGCGAA AGAGGTCCTC CAGGCCCAAA GGGAGAACCC 
GGTCCACCAG GCAAAGGAGT GGAAATGAAG GATCTGGAGA GGCTTTTTGA AGCAAATGGT 
ATCAAGCTGG CACTGCTGAA GGACCTCTCC AATGCACTCC TGCAGGATGG GCTGGATGGG 
CTGGTGTGGC AGCTGGGTGG CTCCAGGACA AGCAAGACCT CCAGGAGAAA GCAAGCAGTT 
CTTCATGCTA CTGAGCATGG CGATTCACTG GTGTTTGATA CTTCTCCTGG TGCCCTGGCC 
AGCACTGAGG TGACAGAAGA AGCACAGAGC CTGGAGGTGG AGGCTCAGTT TAGGGTGCCG 
GTGTCTGGAG GACTCCTTAA GGGTCCATCT GCTGGTGGTT CTGAACCTGA GTACCAGCCC 
CTAGGAAACC CTCCTGAGTC CTTGGAGAGA ACATCTGAGG AAGGAAAGGA GGAGAGAGAC 
CCAAGATGGA CAATCACTTC TCTCAGTGGT AGCTTGGCTT TGCCCCCACT GCACCCAGAA 
GATGATCTGC AAGGAAACCA GTCCATGGAG CTTCTTGAGT CCCTGGAGGG GAGAGTGGAA 
GGACAACCAC AGCAGGACAC TAGCCACGAA AATGAGAATT TAGACGACGG CTCTGCGCTC 
CCAAGGCACG TTAGAGCCCA GAGATCTGCT GAGGACCAGA GACACGTGGT AAAGACCCCA 
TTTCACCACG ACAGCTTCGG GCTGGCCTTT GTGCAAGAGC AGAGCCATCC GTGTGACCCA 
GGAGGAAACG GCGACCTTTC CTTGTTGGCT CCATCACAGG TTCATCCCCC TGGTGCTCCA 
GGAGCTCGGA AATCCCCATC TGGATCCACG GCATACCCAG GCCCTCATCA CCCAAGGAGA 
ATTAAGAAGG AGGAAGCTGG TCCACCAGAT CCACCATCCC ATGGCCAAAA GGGAGAAATG 
GGAGCTCCGG GAGTCCGTGG AGACAAAGGA GAAAAGGGGC AGCCTGGGGA GATGGGGGAA 
CCTGGAGAAA AGGGCATCAA AGGAGACAGA GGTGAAAATG GTCAAAAGGG AGAACCAGGA 
ATTGGGTTCA GAGGCCCTGT TGGTCAGGCT GGACCACCTG GATTTAAAGG TGAGCCAGGG 
GCTCCAGGGC CTCCAGGAGC TCAGGGAGTC CAGGGAATTC GTGGCAATGC CGGCACCCCC 
GGGTCACAAG GGGAAAGAGG GGCTCCAGGT CTGCCTGGGA TGCCAGGACA AAAGGGAGAG 
CGTGGGAAGA GAGGACGGAA TGGATTTGCT GGGCCCTCAG GACCATCAGG ATCCCCAGGA 
AAAGAGGGGA TTTCTGGGAC ACCTGGACCC AAAGGCAACA AGGGAGAAAT CGGCGTTGGA 
GCAGCTGGCC CAAGAGGTCC TCGTGGACTG CCTGGCCCTC GGGGTGACGA GGGCATCGTG 
GGAGTCCGTG GCCCCACCGG CATGACGGGT CTAAAAGGCT TCCCGGGTGT GAAGGGCGAA 
AGAGGTGATC GGGGACTTCT GGGACCCAAA GGAGAAAGAG GTGAGCCCAT GACTATTTTT 
GGACCCCAAG GCTATAAAGG CAGTAAAGGA GATCCTGGTG AACGGGGCCT GCCAGGTTTT 
GACGGAGACA AAGGCGAGAA AGGAGAGGAT GGGCCTATAG GAGAAAAGGG AGTCAAAGGT 
GAAGCTGGCT CCAAGGGAGT GATGGGGCTT TTTGGAACAA GAGGACCAGT GGGACAGAAG 
GGGGAGCCAG GAGAACCAGG CTTGCCGGGC TCTGCTGGTG CAGCAGGTCT TAATGGGAAG 
AACGGAAACA AGGGAGCCAA AGGTGACAGA GGTCTTCAGG GGCAGAAGGG GAAGCCGGGA 
GGAAAAGGTG ATCCTGGGGC CACTGGTGAC ATTGGACAGA AAGGAACAAA GGGCTTACGA 
GGGCTTCCAG GACGCATCGG TGCTCCTGGA GCTGAAGGCA TGAAGGGAGA AATTGGCAGT 
CCTGGAAAAC CAGGAATCCC AGGATCAGAT GGACCACACG GACAAAAGGG TGAAAAGGGA 
ACATCAGGAG ACGATGGTGC CTCGGGGATT CCTGGGGAGA AAGGAGAAAA GGGAGCCAAA 
GGAGTTCCTG GCTTGGGAGG CTTCAAAGGA CAAATGGGAT GGCCTGGGAG GACTGGAGTT 
GCTGGACCAG ATGGCCCTCA AGGGCCACAG GGTGAACCAG GGCCCCAGGG TCCACGAGGG 
AAAAGAGGGA GACCTCAGCT CTGCCTCAGG GGACCCCCAG GCATGGATGG GAGAAAAGGA 
GAAATGGGTG AAAATGGTCC AGCCGGCCAG AAAGGAGAGA AGGGAGATCC TGGTCTTTCT 
GAGGAAGAGG TGAAGGAGGT GGTCAGGAGC GAAATGAGAG GCAGATGCGA TTGTGGAGGC 
GAGTTGGGAA GGACACTCAT GGAAAGGGGC CTGGAGGCAA CTGAAATCAA CAGGCAGCTC 
CAAGGGTTCC AAAAGTTCCA GCTTTGGGAA CGCACCAGAA GGGATGGTGA AAGCCAGGAA 
GAAATGCTGA ACAAAACCCA GGGGATGACC ATGTTGATGA CAATGGTGAA GCCCGTCATG 
GAGCCACCTT CACTTATTGC AGCGGCTCCC CGGGCGCAGC AGATAACAGG TCCCTCTCAG 
CGCAAGCGGC GAGAAGCCCA CAGTCAATTA CCAGCCCCCT GCCTGCAGCC GATGGATGAA 
GGCTCCTGCC AGCACTATGC CCTGCTCTGG TATTACCACC TGGAGGCAAA CACCTGCCGG 
CCCTTCATCT TTGGGGGATG CCGAGGGAAC AGCAACCGCT TCGAAACCAA GTGGAAGTGT 
GAGCAGAAGT GCAAAACCTC AGCAGCCAGA GGGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014816504.1
CDS	249..7727
Translation	MFCAQILALAVAVVQHRAVAVHEACRTAEVADILFLVGQSQGTGRESSWLVKDFISSMARSFENVVMGKGGIRLAVALYGEKPRMSIELTDYVTIEEMLVAIQDLSFKGGSLKTGSALAFAARAMSRLDTLREDAAKVVVLITDGKSSNSVEDEARVLQDLGVTVFAVGIKGADKNELNTIASEPTAEHVLYVEDFHLLHNVAPKLSRRLCFTASEPPRPIKQTVQAEKIVGPRDLYVSEHSHSTLRLTWMPATGKVTGYHVHLHPLLPSGQPVSEDQQQIVVDGDKSTVLVTDLKPNTKYVFTVRAIYADTVGESAVVKGKTTPVPPVTNFRVIEEGLFSLKVAWTPPLGKLEGYKIYIPRANRPGMTYEQVLRGDVSSHVLENLQEDREYSISIYAVYPQGPSQPVAAVGRTLKLLPVKSILLQNETTNTLQARWTPIRGATGYRLTWTSAEGSIQDVNLSNTYSYYMIQGLQPGTEYTVTINPIFGDVEGPVVSSKATTVASSTVQMLKVTEISTNSALVSWDSVAGATGYRVAWGPTPEFFGKDRPRQLALNRSITAYQLRNLAHDTEYVISLYVLFGSVEGPGITTSARTSPLGYVSNFKVMSYTSTSLSLVWSATAAATKFKVTWSPVGAEKEKQVAKTQYLGSRVLAYQIDQLLPNTLYKVSVRAVFNKTEGPEVTLTHRTAPLADSNPIQAVEDLRITDTGVNSLKLSWRRLPGVTHYKISWAPFNGGLETSQVVAAETVSFTISNLKESTTYTICMSAMIGGQEGSPTLLTAKTLDLPKVTEFMVQEAAETSVLLTWTAVPGASTYLLTWRLSSASDLSQELLSAASRSYRVTGLSTKEMYVFSIRPVFGDTEGPETTLLGQTAGPHRESPTPITPVTSTKLGTMRFPSLSPSKVGTTPLPLATTTTVPPTPATLLSTTAVSTLLTTLPGPICGKFKADIGFLVDESSSIGQSNFNKVKDFLFRIISYFPKIGPEGTQVAVAQYSEEPRAAFYFNQHRDRNSALKAVKGLSYAGGNTKTGRGITYMLKELFQSSRGMRPDFPHVLVLVTDGRSQDDVLLPARAAHELGIRVIAVGVSGADPVELNSILLQQNLQNVFYVSTFDDFPQILRELIEVICSDPEPSGPQLPSGESFRSSPAPHGGYDPYSFTTKGEKGERGLPGKDGIPGLPGRPGRTGPPGPPGLMGLPGIQGDTGSPGYPGPAGPKGERGEPGYVLGGVEVIPGRNGQPGPPGQKGQPGVPGVAGPPGLPGPQGPPGISIKGERGDSGTRGPRGRNGLKGDRGGMGEPGKPGLPGPVGLDGVPGLPGPRGEKGVMGPPGAPGPKGNKGQGGVKGEKGLPGPPGQKGDQGNPGFPGAPAMGVLGPPGKKGVRGDIGPTGALGPQGDKGIQGDKGEKGSPGFGIPGQPGLKGDPGDRGNVGLSGKPGQKGDRGLKGEKGEPGLPGKPGETGLRGKDGEAGVPGEPGERGIRGPPGLPGRPGEQGIKGDTGPPGKDGITGEKGDKGEAGEPGLPGTPGSLVSSLESTITLKGDKGDRGKDGLKGERGPPGPKGEPGPPGKGVEMKDLERLFEANGIKLALLKDLSNALLQDGLDGLVWQLGGSRTSKTSRRKQAVLHATEHGDSLVFDTSPGALASTEVTEEAQSLEVEAQFRVPVSGGLLKGPSAGGSEPEYQPLGNPPESLERTSEEGKEERDPRWTITSLSGSLALPPLHPEDDLQGNQSMELLESLEGRVEGQPQQDTSHENENLDDGSALPRHVRAQRSAEDQRHVVKTPFHHDSFGLAFVQEQSHPCDPGGNGDLSLLAPSQVHPPGAPGARKSPSGSTAYPGPHHPRRIKKEEAGPPDPPSHGQKGEMGAPGVRGDKGEKGQPGEMGEPGEKGIKGDRGENGQKGEPGIGFRGPVGQAGPPGFKGEPGAPGPPGAQGVQGIRGNAGTPGSQGERGAPGLPGMPGQKGERGKRGRNGFAGPSGPSGSPGKEGISGTPGPKGNKGEIGVGAAGPRGPRGLPGPRGDEGIVGVRGPTGMTGLKGFPGVKGERGDRGLLGPKGERGEPMTIFGPQGYKGSKGDPGERGLPGFDGDKGEKGEDGPIGEKGVKGEAGSKGVMGLFGTRGPVGQKGEPGEPGLPGSAGAAGLNGKNGNKGAKGDRGLQGQKGKPGGKGDPGATGDIGQKGTKGLRGLPGRIGAPGAEGMKGEIGSPGKPGIPGSDGPHGQKGEKGTSGDDGASGIPGEKGEKGAKGVPGLGGFKGQMGWPGRTGVAGPDGPQGPQGEPGPQGPRGKRGRPQLCLRGPPGMDGRKGEMGENGPAGQKGEKGDPGLSEEEVKEVVRSEMRGRCDCGGELGRTLMERGLEATEINRQLQGFQKFQLWERTRRDGESQEEMLNKTQGMTMLMTMVKPVMEPPSLIAAAPRAQQITGPSQRKRREAHSQLPAPCLQPMDEGSCQHYALLWYYHLEANTCRPFIFGGCRGNSNRFETKWKCEQKCKTSAARGR

Target ORF information:

RefSeq Version	XM_014961018.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax collagen alpha-1(VII) chain-like (LOC106899270), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014961018.1

ORF Insert Sequence:

ATGTTTTGTG CCCAGATCCT TGCCCTGGCA GTGGCTGTGG TGCAGCACAG AGCTGTGGCC 
GTTCATGAAG CATGCAGGAC GGCGGAGGTG GCCGACATTC TGTTCCTCGT GGGACAGTCA 
CAGGGCACAG GAAGGGAGAG CTCCTGGCTG GTCAAGGACT TCATCAGCAG CATGGCTCGT 
TCCTTTGAGA ACGTGGTGAT GGGCAAAGGA GGCATCCGGC TGGCAGTGGC ACTCTACGGG 
GAGAAACCAA GGATGAGTAT TGAGCTTACG GATTACGTGA CCATCGAAGA AATGTTGGTG 
GCAATTCAGG ATCTCTCCTT CAAAGGTGGA AGCTTAAAAA CAGGGTCAGC CTTGGCCTTT 
GCAGCTCGTG CCATGTCTCG TCTGGACACA CTGCGAGAGG ATGCAGCAAA GGTGGTTGTT 
TTGATCACGG ATGGAAAATC CAGCAACTCA GTTGAAGATG AAGCCCGGGT CCTGCAGGAT 
TTGGGGGTGA CGGTGTTTGC CGTAGGGATT AAGGGTGCTG ACAAGAATGA ATTGAACACA 
ATAGCTTCAG AGCCCACTGC AGAGCATGTG CTTTATGTTG AAGATTTCCA CCTCCTCCAT 
AACGTGGCCC CCAAACTCTC TCGAAGGCTC TGTTTCACTG CCTCGGAGCC ACCACGACCC 
ATCAAACAGA CTGTGCAAGC TGAGAAGATC GTCGGCCCCC GGGATTTGTA TGTCAGTGAG 
CACAGCCACA GCACGCTGAG ACTCACCTGG ATGCCAGCCA CGGGGAAGGT GACGGGCTAC 
CATGTCCATC TGCATCCCTT GCTGCCTTCA GGGCAGCCCG TTTCAGAAGA CCAGCAGCAG 
ATTGTGGTGG ACGGTGACAA AAGTACCGTG CTGGTGACTG ATCTGAAACC CAACACCAAG 
TATGTCTTCA CGGTGAGGGC CATCTATGCA GATACTGTTG GGGAGTCAGC GGTTGTCAAG 
GGGAAAACAA CACCTGTGCC TCCAGTCACT AATTTCCGTG TGATAGAAGA AGGACTTTTT 
AGCTTGAAGG TGGCTTGGAC ACCTCCGCTT GGCAAGCTGG AGGGCTACAA GATCTACATC 
CCCAGAGCCA ACAGACCAGG AATGACCTAC GAGCAGGTTC TGCGGGGTGA TGTCTCATCC 
CACGTGCTGG AGAACTTGCA GGAGGACAGA GAATACAGCA TCAGCATCTA CGCCGTGTAC 
CCCCAGGGCC CCAGCCAGCC TGTGGCTGCT GTGGGGAGAA CATTGAAGCT CCTGCCCGTG 
AAAAGCATCT TGCTGCAGAA TGAGACCACC AACACGCTCC AGGCAAGGTG GACACCCATC 
CGGGGAGCAA CGGGGTACAG GCTCACCTGG ACATCAGCAG AGGGCAGCAT CCAAGATGTG 
AACCTCAGCA ACACCTACAG CTACTACATG ATCCAGGGGC TGCAGCCTGG CACGGAGTAC 
ACTGTTACCA TCAACCCCAT CTTTGGGGAC GTCGAGGGAC CAGTGGTGAG CAGCAAAGCA 
ACGACGGTGG CATCGAGCAC CGTCCAGATG CTGAAAGTGA CTGAGATATC TACCAACAGT 
GCTCTGGTCT CCTGGGACTC AGTTGCTGGG GCCACTGGGT ATCGAGTGGC TTGGGGTCCT 
ACACCAGAGT TCTTTGGCAA AGACCGTCCT CGCCAGCTGG CCCTCAACAG GTCAATCACA 
GCCTACCAGC TCCGCAACCT GGCCCATGAC ACCGAGTACG TGATTTCCCT CTACGTGCTC 
TTTGGCTCCG TGGAAGGACC AGGGATCACA ACCTCAGCCA GGACATCTCC TCTTGGCTAC 
GTCTCCAATT TCAAGGTGAT GTCCTACACC AGCACAAGCC TGTCTCTGGT GTGGAGTGCC 
ACAGCAGCTG CCACCAAATT CAAAGTCACC TGGAGCCCTG TGGGAGCAGA AAAAGAAAAA 
CAGGTTGCCA AGACTCAGTA CCTGGGCAGT AGGGTGCTGG CTTACCAGAT TGACCAGTTA 
CTGCCCAACA CCCTGTATAA AGTCAGCGTT CGGGCTGTCT TCAACAAGAC TGAGGGACCA 
GAGGTGACTC TGACTCACCG CACAGCGCCT CTTGCAGACT CCAACCCCAT CCAGGCCGTT 
GAAGATCTGA GGATTACTGA CACCGGCGTA AATAGCTTGA AGCTGTCTTG GAGGAGACTT 
CCTGGGGTAA CTCATTATAA GATATCCTGG GCGCCGTTCA ATGGTGGCTT GGAAACCTCC 
CAGGTGGTTG CAGCAGAGAC GGTCTCCTTC ACGATCTCCA ACCTGAAGGA GAGCACCACC 
TACACCATCT GCATGTCTGC AATGATCGGG GGACAGGAGG GAAGCCCCAC ACTCCTCACA 
GCCAAAACTT TGGATTTACC CAAAGTGACT GAGTTCATGG TGCAGGAGGC TGCGGAGACC 
AGCGTTTTGC TGACATGGAC AGCTGTTCCT GGGGCATCCA CTTATCTATT AACATGGCGC 
CTGTCCTCAG CTTCTGATCT TTCCCAGGAA CTGCTGTCAG CAGCTTCTAG ATCGTATCGA 
GTGACAGGAC TGAGCACCAA AGAAATGTAT GTGTTTTCCA TAAGACCAGT CTTTGGAGAT 
ACGGAGGGAC CAGAGACAAC ACTCCTTGGG CAAACAGCGG GACCTCACAG AGAGTCACCC 
ACACCCATCA CTCCAGTGAC TTCTACAAAA CTGGGTACGA TGAGATTCCC ATCCTTATCT 
CCTAGCAAGG TGGGGACCAC TCCTCTTCCA CTGGCCACCA CCACAACAGT ACCACCAACT 
CCAGCAACCC TACTGTCGAC CACTGCAGTG TCCACACTGC TGACAACTCT CCCTGGGCCA 
ATCTGTGGCA AGTTCAAAGC AGACATTGGG TTCCTGGTGG ACGAGTCCTC GAGTATTGGC 
CAGAGCAATT TCAACAAAGT GAAGGATTTC CTCTTCCGGA TCATTTCCTA TTTCCCGAAG 
ATTGGGCCTG AAGGGACACA GGTTGCTGTT GCTCAGTACA GCGAGGAGCC CAGGGCAGCC 
TTCTACTTCA ACCAGCACCG GGACCGCAAC AGTGCCCTGA AAGCCGTCAA GGGACTGAGC 
TACGCTGGGG GCAACACAAA AACAGGTCGT GGCATAACCT ACATGCTCAA GGAGTTATTT 
CAGTCCTCCA GAGGGATGAG GCCAGATTTC CCTCACGTGC TGGTGCTGGT GACCGATGGG 
CGATCCCAGG ATGATGTGTT GCTACCAGCC AGAGCAGCCC ATGAGTTGGG GATCCGCGTC 
ATTGCTGTGG GTGTCTCAGG AGCTGACCCC GTTGAGCTCA ACAGCATCTT GCTGCAGCAG 
AACCTCCAGA ATGTTTTCTA CGTCAGCACG TTTGATGACT TCCCCCAGAT CCTCCGAGAG 
CTGATAGAGG TCATCTGTTC TGACCCCGAG CCTTCAGGGC CTCAACTACC ATCTGGGGAG 
AGCTTCAGGA GTTCACCAGC TCCTCATGGA GGCTATGACC CATACAGCTT TACTACAAAG 
GGAGAGAAAG GAGAGAGGGG TCTACCTGGG AAGGATGGAA TTCCCGGGCT GCCGGGCAGA 
CCGGGGCGGA CAGGTCCTCC TGGTCCCCCT GGACTCATGG GTCTCCCTGG CATCCAAGGT 
GATACTGGCT CTCCTGGCTA TCCTGGACCA GCGGGGCCAA AAGGAGAGAG AGGCGAACCA 
GGCTATGTCC TGGGAGGAGT AGAGGTGATT CCTGGCCGAA ATGGGCAGCC TGGCCCCCCT 
GGACAGAAAG GGCAGCCGGG GGTTCCTGGG GTAGCAGGAC CACCAGGTTT ACCAGGGCCA 
CAGGGGCCAC CAGGCATCTC CATCAAGGGT GAACGTGGGG ATTCAGGTAC CAGGGGCCCT 
CGTGGGAGGA ATGGCCTCAA GGGTGACAGA GGAGGAATGG GAGAGCCGGG GAAACCTGGG 
TTGCCAGGCC CAGTAGGGCT AGATGGTGTT CCTGGATTGC CTGGACCAAG GGGAGAAAAG 
GGTGTGATGG GACCTCCAGG AGCACCAGGA CCAAAGGGTA ACAAAGGGCA AGGTGGTGTG 
AAAGGGGAAA AGGGACTCCC AGGACCTCCA GGGCAGAAGG GAGACCAGGG GAACCCAGGA 
TTTCCAGGTG CTCCAGCTAT GGGGGTTTTG GGACCTCCAG GCAAGAAAGG TGTCAGGGGT 
GATATTGGAC CAACAGGGGC CCTGGGACCA CAAGGAGACA AAGGAATACA AGGAGACAAA 
GGAGAGAAGG GAAGCCCAGG TTTCGGGATT CCTGGTCAAC CTGGGCTGAA GGGAGATCCA 
GGCGACAGGG GAAATGTAGG TTTGTCGGGC AAACCAGGTC AGAAGGGGGA TAGAGGTCTG 
AAGGGAGAGA AAGGTGAACC AGGACTACCT GGGAAACCAG GCGAGACCGG ACTAAGAGGT 
AAAGATGGAG AAGCTGGTGT CCCTGGAGAA CCAGGAGAGA GAGGAATCAG GGGGCCACCT 
GGACTCCCAG GACGGCCTGG AGAGCAAGGG ATAAAAGGCG ATACAGGACC ACCTGGAAAG 
GATGGAATCA CTGGAGAGAA GGGAGACAAG GGCGAAGCTG GTGAGCCTGG GCTGCCTGGG 
ACTCCTGGAA GCCTTGTGTC TTCACTGGAG AGCACCATCA CCCTGAAAGG CGACAAGGGG 
GATCGTGGGA AGGATGGCTT GAAAGGCGAA AGAGGTCCTC CAGGCCCAAA GGGAGAACCC 
GGTCCACCAG GCAAAGGAGT GGAAATGAAG GATCTGGAGA GGCTTTTTGA AGCAAATGGT 
ATCAAGCTGG CACTGCTGAA GGACCTCTCC AATGCACTCC TGCAGGATGG GCTGGATGGG 
CTGGTGTGGC AGCTGGGTGG CTCCAGGACA AGCAAGACCT CCAGGAGAAA GCAAGCAGTT 
CTTCATGCTA CTGAGCATGG CGATTCACTG GTGTTTGATA CTTCTCCTGG TGCCCTGGCC 
AGCACTGAGG TGACAGAAGA AGCACAGAGC CTGGAGGTGG AGGCTCAGTT TAGGGTGCCG 
GTGTCTGGAG GACTCCTTAA GGGTCCATCT GCTGGTGGTT CTGAACCTGA GTACCAGCCC 
CTAGGAAACC CTCCTGAGTC CTTGGAGAGA ACATCTGAGG AAGGAAAGGA GGAGAGAGAC 
CCAAGATGGA CAATCACTTC TCTCAGTGGT AGCTTGGCTT TGCCCCCACT GCACCCAGAA 
GATGATCTGC AAGGAAACCA GTCCATGGAG CTTCTTGAGT CCCTGGAGGG GAGAGTGGAA 
GGACAACCAC AGCAGGACAC TAGCCACGAA AATGAGAATT TAGACGACGG CTCTGCGCTC 
CCAAGGCACG TTAGAGCCCA GAGATCTGCT GAGGACCAGA GACACGTGGT AAAGACCCCA 
TTTCACCACG ACAGCTTCGG GCTGGCCTTT GTGCAAGAGC AGAGCCATCC GTGTGACCCA 
GGAGGAAACG GCGACCTTTC CTTGTTGGCT CCATCACAGG TTCATCCCCC TGGTGCTCCA 
GGAGCTCGGA AATCCCCATC TGGATCCACG GCATACCCAG GCCCTCATCA CCCAAGGAGA 
ATTAAGAAGG AGGAAGCTGG TCCACCAGAT CCACCATCCC ATGGCCAAAA GGGAGAAATG 
GGAGCTCCGG GAGTCCGTGG AGACAAAGGA GAAAAGGGGC AGCCTGGGGA GATGGGGGAA 
CCTGGAGAAA AGGGCATCAA AGGAGACAGA GGTGAAAATG GTCAAAAGGG AGAACCAGGA 
ATTGGGTTCA GAGGCCCTGT TGGTCAGGCT GGACCACCTG GATTTAAAGG TGAGCCAGGG 
GCTCCAGGGC CTCCAGGAGC TCAGGGAGTC CAGGGAATTC GTGGCAATGC CGGCACCCCC 
GGGTCACAAG GGGAAAGAGG GGCTCCAGGT CTGCCTGGGA TGCCAGGACA AAAGGGAGAG 
CGTGGGAAGA GAGGACGGAA TGGATTTGCT GGGCCCTCAG GACCATCAGG ATCCCCAGGA 
AAAGAGGGGA TTTCTGGGAC ACCTGGACCC AAAGGCAACA AGGGAGAAAT CGGCGTTGGA 
GCAGCTGGCC CAAGAGGTCC TCGTGGACTG CCTGGCCCTC GGGGTGACGA GGGCATCGTG 
GGAGTCCGTG GCCCCACCGG CATGACGGGT CTAAAAGGCT TCCCGGGTGT GAAGGGCGAA 
AGAGGTGATC GGGGACTTCT GGGACCCAAA GGAGAAAGAG GTGAGCCCAT GACTATTTTT 
GGACCCCAAG GCTATAAAGG CAGTAAAGGA GATCCTGGTG AACGGGGCCT GCCAGGTTTT 
GACGGAGACA AAGGCGAGAA AGGAGAGGAT GGGCCTATAG GAGAAAAGGG AGTCAAAGGT 
GAAGCTGGCT CCAAGGGAGT GATGGGGCTT TTTGGAACAA GAGGACCAGT GGGACAGAAG 
GGGGAGCCAG GAGAACCAGG CTTGCCGGGC TCTGCTGGTG CAGCAGGTCT TAATGGGAAG 
AACGGAAACA AGGGAGCCAA AGGTGACAGA GGTCTTCAGG GGCAGAAGGG GAAGCCGGGA 
GGAAAAGGTG ATCCTGGGGC CACTGGTGAC ATTGGACAGA AAGGAACAAA GGGCTTACGA 
GGGCTTCCAG GACGCATCGG TGCTCCTGGA GCTGAAGGCA TGAAGGGAGA AATTGGCAGT 
CCTGGAAAAC CAGGAATCCC AGGATCAGAT GGACCACACG GACAAAAGGG TGAAAAGGGA 
ACATCAGGAG ACGATGGTGC CTCGGGGATT CCTGGGGAGA AAGGAGAAAA GGGAGCCAAA 
GGAGTTCCTG GCTTGGGAGG CTTCAAAGGA CAAATGGGAT GGCCTGGGAG GACTGGAGTT 
GCTGGACCAG ATGGCCCTCA AGGGCCACAG GGTGAACCAG GGCCCCAGGG TCCACGAGGG 
AAAAGAGGGA GACCTCAGCT CTGCCTCAGG GGACCCCCAG GCATGGATGG GAGAAAAGGA 
GAAATGGGTG AAAATGGTCC AGCCGGCCAG AAAGGAGAGA AGGGAGATCC TGGTCTTTCT 
GAGGAAGAGG TGAAGGAGGT GGTCAGGAGC GAAATGAGAG GCAGATGCGA TTGTGGAGGC 
GAGTTGGGAA GGACACTCAT GGAAAGGGGC CTGGAGGCAA CTGAAATCAA CAGGCAGCTC 
CAAGGGTTCC AAAAGTTCCA GCTTTGGGAA CGCACCAGAA GGGATGGTGA AAGCCAGGAA 
GAAATGCTGA ACAAAACCCA GGGGATGACC ATGTTGATGA CAATGGTGAA GCCCGTCATG 
GAGCCACCTT CACTTATTGC AGCGGCTCCC CGGGCGCAGC AGATAACAGG TCCCTCTCAG 
CGCAAGCGGC GAGAAGCCCA CAGTCAATTA CCAGCCCCCT GCCTGCAGCC GATGGATGAA 
GGCTCCTGCC AGCACTATGC CCTGCTCTGG TATTACCACC TGGAGGCAAA CACCTGCCGG 
CCCTTCATCT TTGGGGGATG CCGAGGGAAC AGCAACCGCT TCGAAACCAA GTGGAAGTGT 
GAGCAGAAGT GCAAAACCTC AGCAGCCAGA GGGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106899270 ( XM_014961019.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014816505.1 Calidris pugnax collagen alpha-1(VII) chain-like (LOC106899270), transcript variant X5, mRNA.

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CloneID	OCa78936
Clone ID Related Accession (Same CDS sequence)	XM_014961019.1
Accession Version	XM_014961019.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	collagen alpha-1(VII) chain-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTGTG CCCAGATCCT TGCCCTGGCA GTGGCTGTGG TGCAGCACAG AGCTGTGGCC 
GTTCATGAAG CATGCAGGAC GGCGGAGGTG GCCGACATTC TGTTCCTCGT GGGACAGTCA 
CAGGGCACAG GAAGGGAGAG CTCCTGGCTG GTCAAGGACT TCATCAGCAG CATGGCTCGT 
TCCTTTGAGA ACGTGGTGAT GGGCAAAGGA GGCATCCGGC TGGCAGTGGC ACTCTACGGG 
GAGAAACCAA GGATGAGTAT TGAGCTTACG GATTACGTGA CCATCGAAGA AATGTTGGTG 
GCAATTCAGG ATCTCTCCTT CAAAGGTGGA AGCTTAAAAA CAGGGTCAGC CTTGGCCTTT 
GCAGCTCGTG CCATGTCTCG TCTGGACACA CTGCGAGAGG ATGCAGCAAA GGTGGTTGTT 
TTGATCACGG ATGGAAAATC CAGCAACTCA GTTGAAGATG AAGCCCGGGT CCTGCAGGAT 
TTGGGGGTGA CGGTGTTTGC CGTAGGGATT AAGGGTGCTG ACAAGAATGA ATTGAACACA 
ATAGCTTCAG AGCCCACTGC AGAGCATGTG CTTTATGTTG AAGATTTCCA CCTCCTCCAT 
AACGTGGCCC CCAAACTCTC TCGAAGGCTC TGTTTCACTG CCTCGGAGCC ACCACGACCC 
ATCAAACAGA CTGTGCAAGC TGAGAAGATC GTCGGCCCCC GGGATTTGTA TGTCAGTGAG 
CACAGCCACA GCACGCTGAG ACTCACCTGG ATGCCAGCCA CGGGGAAGGT GACGGGCTAC 
CATGTCCATC TGCATCCCTT GCTGCCTTCA GGGCAGCCCG TTTCAGAAGA CCAGCAGCAG 
ATTGTGGTGG ACGGTGACAA AAGTACCGTG CTGGTGACTG ATCTGAAACC CAACACCAAG 
TATGTCTTCA CGGTGAGGGC CATCTATGCA GATACTGTTG GGGAGTCAGC GGTTGTCAAG 
GGGAAAACAA CACCTGTGCC TCCAGTCACT AATTTCCGTG TGATAGAAGA AGGACTTTTT 
AGCTTGAAGG TGGCTTGGAC ACCTCCGCTT GGCAAGCTGG AGGGCTACAA GATCTACATC 
CCCAGAGCCA ACAGACCAGG AATGACCTAC GAGCAGGTTC TGCGGGGTGA TGTCTCATCC 
CACGTGCTGG AGAACTTGCA GGAGGACAGA GAATACAGCA TCAGCATCTA CGCCGTGTAC 
CCCCAGGGCC CCAGCCAGCC TGTGGCTGCT GTGGGGAGAA CATTGAAGCT CCTGCCCGTG 
AAAAGCATCT TGCTGCAGAA TGAGACCACC AACACGCTCC AGGCAAGGTG GACACCCATC 
CGGGGAGCAA CGGGGTACAG GCTCACCTGG ACATCAGCAG AGGGCAGCAT CCAAGATGTG 
AACCTCAGCA ACACCTACAG CTACTACATG ATCCAGGGGC TGCAGCCTGG CACGGAGTAC 
ACTGTTACCA TCAACCCCAT CTTTGGGGAC GTCGAGGGAC CAGTGGTGAG CAGCAAAGCA 
ACGACGGTGG CATCGAGCAC CGTCCAGATG CTGAAAGTGA CTGAGATATC TACCAACAGT 
GCTCTGGTCT CCTGGGACTC AGTTGCTGGG GCCACTGGGT ATCGAGTGGC TTGGGGTCCT 
ACACCAGAGT TCTTTGGCAA AGACCGTCCT CGCCAGCTGG CCCTCAACAG GTCAATCACA 
GCCTACCAGC TCCGCAACCT GGCCCATGAC ACCGAGTACG TGATTTCCCT CTACGTGCTC 
TTTGGCTCCG TGGAAGGACC AGGGATCACA ACCTCAGCCA GGACATCTCC TCTTGGCTAC 
GTCTCCAATT TCAAGGTGAT GTCCTACACC AGCACAAGCC TGTCTCTGGT GTGGAGTGCC 
ACAGCAGCTG CCACCAAATT CAAAGTCACC TGGAGCCCTG TGGGAGCAGA AAAAGAAAAA 
CAGGTTGCCA AGACTCAGTA CCTGGGCAGT AGGGTGCTGG CTTACCAGAT TGACCAGTTA 
CTGCCCAACA CCCTGTATAA AGTCAGCGTT CGGGCTGTCT TCAACAAGAC TGAGGGACCA 
GAGGTGACTC TGACTCACCG CACAGCGCCT CTTGCAGACT CCAACCCCAT CCAGGCCGTT 
GAAGATCTGA GGATTACTGA CACCGGCGTA AATAGCTTGA AGCTGTCTTG GAGGAGACTT 
CCTGGGGTAA CTCATTATAA GATATCCTGG GCGCCGTTCA ATGGTGGCTT GGAAACCTCC 
CAGGTGGTTG CAGCAGAGAC GGTCTCCTTC ACGATCTCCA ACCTGAAGGA GAGCACCACC 
TACACCATCT GCATGTCTGC AATGATCGGG GGACAGGAGG GAAGCCCCAC ACTCCTCACA 
GCCAAAACTT TGGATTTACC CAAAGTGACT GAGTTCATGG TGCAGGAGGC TGCGGAGACC 
AGCGTTTTGC TGACATGGAC AGCTGTTCCT GGGGCATCCA CTTATCTATT AACATGGCGC 
CTGTCCTCAG CTTCTGATCT TTCCCAGGAA CTGCTGTCAG CAGCTTCTAG ATCGTATCGA 
GTGACAGGAC TGAGCACCAA AGAAATGTAT GTGTTTTCCA TAAGACCAGT CTTTGGAGAT 
ACGGAGGGAC CAGAGACAAC ACTCCTTGGG CAAACAGCGG GACCTCACAG AGAGTCACCC 
ACACCCATCA CTCCAGTGAC TTCTACAAAA CTGGGTACGA TGAGATTCCC ATCCTTATCT 
CCTAGCAAGG TGGGGACCAC TCCTCTTCCA CTGGCCACCA CCACAACAGT ACCACCAACT 
CCAGCAACCC TACTGTCGAC CACTGCAGTG TCCACACTGC TGACAACTCT CCCTGGGCCA 
ATCTGTGGCA AGTTCAAAGC AGACATTGGG TTCCTGGTGG ACGAGTCCTC GAGTATTGGC 
CAGAGCAATT TCAACAAAGT GAAGGATTTC CTCTTCCGGA TCATTTCCTA TTTCCCGAAG 
ATTGGGCCTG AAGGGACACA GGTTGCTGTT GCTCAGTACA GCGAGGAGCC CAGGGCAGCC 
TTCTACTTCA ACCAGCACCG GGACCGCAAC AGTGCCCTGA AAGCCGTCAA GGGACTGAGC 
TACGCTGGGG GCAACACAAA AACAGGTCGT GGCATAACCT ACATGCTCAA GGAGTTATTT 
CAGTCCTCCA GAGGGATGAG GCCAGATTTC CCTCACGTGC TGGTGCTGGT GACCGATGGG 
CGATCCCAGG ATGATGTGTT GCTACCAGCC AGAGCAGCCC ATGAGTTGGG GATCCGCGTC 
ATTGCTGTGG GTGTCTCAGG AGCTGACCCC GTTGAGCTCA ACAGCATCTT GCTGCAGCAG 
AACCTCCAGA ATGTTTTCTA CGTCAGCACG TTTGATGACT TCCCCCAGAT CCTCCGAGAG 
CTGATAGAGG TCATCTGTTC TGACCCCGAG CCTTCAGGGC CTCAACTACC ATCTGGGGAG 
AGCTTCAGGA GTTCACCAGC TCCTCATGGA GGCTATGACC CATACAGCTT TACTACAAAG 
GGAGAGAAAG GAGAGAGGGG TCTACCTGGG AAGGATGGAA TTCCCGGGCT GCCGGGCAGA 
CCGGGGCGGA CAGGTCCTCC TGGTCCCCCT GGACTCATGG GTCTCCCTGG CATCCAAGGT 
GATACTGGCT CTCCTGGCTA TCCTGGACCA GCGGGGCCAA AAGGAGAGAG AGGCGAACCA 
GGCTATGTCC TGGGAGGAGT AGAGGTGATT CCTGGCCGAA ATGGGCAGCC TGGCCCCCCT 
GGACAGAAAG GGCAGCCGGG GGTTCCTGGG GTAGCAGGAC CACCAGGTTT ACCAGGGCCA 
CAGGGGCCAC CAGGCATCTC CATCAAGGGT GAACGTGGGG ATTCAGGTAC CAGGGGCCCT 
CGTGGGAGGA ATGGCCTCAA GGGTGACAGA GGAGGAATGG GAGAGCCGGG GAAACCTGGG 
TTGCCAGGCC CAGTAGGGCT AGATGGTGTT CCTGGATTGC CTGGACCAAG GGGAGAAAAG 
GGTGTGATGG GACCTCCAGG AGCACCAGGA CCAAAGGGTA ACAAAGGGCA AGGTGGTGTG 
AAAGGGGAAA AGGGACTCCC AGGACCTCCA GGGCAGAAGG GAGACCAGGG GAACCCAGGA 
TTTCCAGGTG CTCCAGCTAT GGGGGTTTTG GGACCTCCAG GCAAGAAAGG TGTCAGGGGT 
GATATTGGAC CAACAGGGGC CCTGGGACCA CAAGGAGACA AAGGAATACA AGGAGACAAA 
GGAGAGAAGG GAAGCCCAGG TTTCGGGATT CCTGGTCAAC CTGGGCTGAA GGGAGATCCA 
GGCGACAGGG GAAATGTAGG TTTGTCGGGC AAACCAGGTC AGAAGGGGGA TAGAGGTCTG 
AAGGGAGAGA AAGGTGAACC AGGACTACCT GGGAAACCAG GCGAGACCGG ACTAAGAGGT 
AAAGATGGAG AACCAGGGGA GAAAGGCACC TCTGGGACCA AGGGAGAAGC TGGTGTCCCT 
GGAGAACCAG GAGAGAGAGG AATCAGGGGG CCACCTGGAC TCCCAGGACG GCCTGGAGAG 
CAAGGGATAA AAGGCGATAC AGGACCACCT GGAAAGGATG GAATCACTGG AGAGAAGGGA 
GACAAGGGCG AAGCTGGTGA GCCTGGGCTG CCTGGGACTC CTGGAAGCCT TGTGTCTTCA 
CTGGAGAGCA CCATCACCCT GAAAGGCGAC AAGGGGGATC GTGGGAAGGA TGGCTTGAAA 
GGCGAAAGAG GTCCTCCAGG CCCAAAGGGA GAACCCGGTC CACCAGGCAA AGGAGTGGAA 
ATGAAGGATC TGGAGAGGCT TTTTGAAGCA AATGGTATCA AGCTGGCACT GCTGAAGGAC 
CTCTCCAATG CACTCCTGCA GGATGGGCTG GATGGGCTGG TGTGGCAGCT GGGTGGCTCC 
AGGACAAGCA AGACCTCCAG GAGAAAGCAA GCAGTTCTTC ATGCTACTGA GCATGGCGAT 
TCACTGGTGT TTGATACTTC TCCTGGTGCC CTGGCCAGCA CTGAGGTGAC AGAAGAAGCA 
CAGAGCCTGG AGGTGGAGGC TCAGTTTAGG GTGCCGGTGT CTGGAGGACT CCTTAAGGGT 
CCATCTGCTG GTGGTTCTGA ACCTGAGTAC CAGCCCCTAG GAAACCCTCC TGAGTCCTTG 
GAGAGAACAT CTGAGGAAGG AAAGGAGGAG AGAGACCCAA GATGGACAAT CACTTCTCTC 
AGTGGTAGCT TGGCTTTGCC CCCACTGCAC CCAGAAGATG ATCTGCAAGG AAACCAGTCC 
ATGGAGCTTC TTGAGTCCCT GGAGGGGAGA GTGGAAGGAC AACCACAGCA GGACACTAGC 
CACGAAAATG AGAATTTAGA CGACGGCTCT GCGCTCCCAA GGCACGTTAG AGCCCAGAGA 
TCTGCTGAGG ACCAGAGACA CGTGGTTCAT CCCCCTGGTG CTCCAGGAGC TCGGAAATCC 
CCATCTGGAT CCACGGCATA CCCAGGCCCT CATCACCCAA GGAGAATTAA GAAGGAGGAA 
GCTGGTCCAC CAGATCCACC ATCCCATGGC CAAAAGGGAG AAATGGGAGC TCCGGGAGTC 
CGTGGAGACA AAGGAGAAAA GGGGCAGCCT GGGGAGATGG GGGAACCTGG AGAAAAGGGC 
ATCAAAGGAG ACAGAGGTGA AAATGGTCAA AAGGGAGAAC CAGGAATTGG GTTCAGAGGC 
CCTGTTGGTC AGGCTGGACC ACCTGGATTT AAAGGTGAGC CAGGGGCTCC AGGGCCTCCA 
GGAGCTCAGG GAGTCCAGGG AATTCGTGGC AATGCCGGCA CCCCCGGGTC ACAAGGGGAA 
AGAGGGGCTC CAGGTCTGCC TGGGATGCCA GGACAAAAGG GAGAGCGTGG GAAGAGAGGA 
CGGAATGGAT TTGCTGGGCC CTCAGGACCA TCAGGATCCC CAGGAAAAGA GGGGATTTCT 
GGGACACCTG GACCCAAAGG CAACAAGGGA GAAATCGGCG TTGGAGCAGC TGGCCCAAGA 
GGTCCTCGTG GACTGCCTGG CCCTCGGGGT GACGAGGGCA TCGTGGGAGT CCGTGGCCCC 
ACCGGCATGA CGGGTCTAAA AGGCTTCCCG GGTGTGAAGG GCGAAAGAGG TGATCGGGGA 
CTTCTGGGAC CCAAAGGAGA AAGAGGTGAG CCCATGACTA TTTTTGGACC CCAAGGCTAT 
AAAGGCAGTA AAGGAGATCC TGGTGAACGG GGCCTGCCAG GTTTTGACGG AGACAAAGGC 
GAGAAAGGAG AGGATGGGCC TATAGGAGAA AAGGGAGTCA AAGGTGAAGC TGGCTCCAAG 
GGAGTGATGG GGCTTTTTGG AACAAGAGGA CCAGTGGGAC AGAAGGGGGA GCCAGGAGAA 
CCAGGCTTGC CGGGCTCTGC TGGTGCAGCA GGTCTTAATG GGAAGAACGG AAACAAGGGA 
GCCAAAGGTG ACAGAGGTCT TCAGGGGCAG AAGGGGAAGC CGGGAGGAAA AGGTGATCCT 
GGGGCCACTG GTGACATTGG ACAGAAAGGA ACAAAGGGCT TACGAGGGCT TCCAGGACGC 
ATCGGTGCTC CTGGAGCTGA AGGCATGAAG GGAGAAATTG GCAGTCCTGG AAAACCAGGA 
ATCCCAGGAT CAGATGGACC ACACGGACAA AAGGGTGAAA AGGGAACATC AGGAGACGAT 
GGTGCCTCGG GGATTCCTGG GGAGAAAGGA GAAAAGGGAG CCAAAGGAGT TCCTGGCTTG 
GGAGGCTTCA AAGGACAAAT GGGATGGCCT GGGAGGACTG GAGTTGCTGG ACCAGATGGC 
CCTCAAGGGC CACAGGGTGA ACCAGGGCCC CAGGGTCCAC GAGGGAAAAG AGGGAGACCT 
CAGCTCTGCC TCAGGGGACC CCCAGGCATG GATGGGAGAA AAGGAGAAAT GGGTGAAAAT 
GGTCCAGCCG GCCAGAAAGG AGAGAAGGGA GATCCTGGTC TTTCTGAGGA AGAGGTGAAG 
GAGGTGGTCA GGAGCGAAAT GAGAGGCAGA TGCGATTGTG GAGGCGAGTT GGGAAGGACA 
CTCATGGAAA GGGGCCTGGA GGCAACTGAA ATCAACAGGC AGCTCCAAGG GTTCCAAAAG 
TTCCAGCTTT GGGAACGCAC CAGAAGGGAT GGTGAAAGCC AGGAAGAAAT GCTGAACAAA 
ACCCAGGGGA TGACCATGTT GATGACAATG GTGAAGCCCG TCATGGAGCC ACCTTCACTT 
ATTGCAGCGG CTCCCCGGGC GCAGCAGATA ACAGGTCCCT CTCAGCGCAA GCGGCGAGAA 
GCCCACAGTC AATTACCAGC CCCCTGCCTG CAGCCGATGG ATGAAGGCTC CTGCCAGCAC 
TATGCCCTGC TCTGGTATTA CCACCTGGAG GCAAACACCT GCCGGCCCTT CATCTTTGGG 
GGATGCCGAG GGAACAGCAA CCGCTTCGAA ACCAAGTGGA AGTGTGAGCA GAAGTGCAAA 
ACCTCAGCAG CCAGAGGGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014816505.1
CDS	249..7652
Translation	MFCAQILALAVAVVQHRAVAVHEACRTAEVADILFLVGQSQGTGRESSWLVKDFISSMARSFENVVMGKGGIRLAVALYGEKPRMSIELTDYVTIEEMLVAIQDLSFKGGSLKTGSALAFAARAMSRLDTLREDAAKVVVLITDGKSSNSVEDEARVLQDLGVTVFAVGIKGADKNELNTIASEPTAEHVLYVEDFHLLHNVAPKLSRRLCFTASEPPRPIKQTVQAEKIVGPRDLYVSEHSHSTLRLTWMPATGKVTGYHVHLHPLLPSGQPVSEDQQQIVVDGDKSTVLVTDLKPNTKYVFTVRAIYADTVGESAVVKGKTTPVPPVTNFRVIEEGLFSLKVAWTPPLGKLEGYKIYIPRANRPGMTYEQVLRGDVSSHVLENLQEDREYSISIYAVYPQGPSQPVAAVGRTLKLLPVKSILLQNETTNTLQARWTPIRGATGYRLTWTSAEGSIQDVNLSNTYSYYMIQGLQPGTEYTVTINPIFGDVEGPVVSSKATTVASSTVQMLKVTEISTNSALVSWDSVAGATGYRVAWGPTPEFFGKDRPRQLALNRSITAYQLRNLAHDTEYVISLYVLFGSVEGPGITTSARTSPLGYVSNFKVMSYTSTSLSLVWSATAAATKFKVTWSPVGAEKEKQVAKTQYLGSRVLAYQIDQLLPNTLYKVSVRAVFNKTEGPEVTLTHRTAPLADSNPIQAVEDLRITDTGVNSLKLSWRRLPGVTHYKISWAPFNGGLETSQVVAAETVSFTISNLKESTTYTICMSAMIGGQEGSPTLLTAKTLDLPKVTEFMVQEAAETSVLLTWTAVPGASTYLLTWRLSSASDLSQELLSAASRSYRVTGLSTKEMYVFSIRPVFGDTEGPETTLLGQTAGPHRESPTPITPVTSTKLGTMRFPSLSPSKVGTTPLPLATTTTVPPTPATLLSTTAVSTLLTTLPGPICGKFKADIGFLVDESSSIGQSNFNKVKDFLFRIISYFPKIGPEGTQVAVAQYSEEPRAAFYFNQHRDRNSALKAVKGLSYAGGNTKTGRGITYMLKELFQSSRGMRPDFPHVLVLVTDGRSQDDVLLPARAAHELGIRVIAVGVSGADPVELNSILLQQNLQNVFYVSTFDDFPQILRELIEVICSDPEPSGPQLPSGESFRSSPAPHGGYDPYSFTTKGEKGERGLPGKDGIPGLPGRPGRTGPPGPPGLMGLPGIQGDTGSPGYPGPAGPKGERGEPGYVLGGVEVIPGRNGQPGPPGQKGQPGVPGVAGPPGLPGPQGPPGISIKGERGDSGTRGPRGRNGLKGDRGGMGEPGKPGLPGPVGLDGVPGLPGPRGEKGVMGPPGAPGPKGNKGQGGVKGEKGLPGPPGQKGDQGNPGFPGAPAMGVLGPPGKKGVRGDIGPTGALGPQGDKGIQGDKGEKGSPGFGIPGQPGLKGDPGDRGNVGLSGKPGQKGDRGLKGEKGEPGLPGKPGETGLRGKDGEPGEKGTSGTKGEAGVPGEPGERGIRGPPGLPGRPGEQGIKGDTGPPGKDGITGEKGDKGEAGEPGLPGTPGSLVSSLESTITLKGDKGDRGKDGLKGERGPPGPKGEPGPPGKGVEMKDLERLFEANGIKLALLKDLSNALLQDGLDGLVWQLGGSRTSKTSRRKQAVLHATEHGDSLVFDTSPGALASTEVTEEAQSLEVEAQFRVPVSGGLLKGPSAGGSEPEYQPLGNPPESLERTSEEGKEERDPRWTITSLSGSLALPPLHPEDDLQGNQSMELLESLEGRVEGQPQQDTSHENENLDDGSALPRHVRAQRSAEDQRHVVHPPGAPGARKSPSGSTAYPGPHHPRRIKKEEAGPPDPPSHGQKGEMGAPGVRGDKGEKGQPGEMGEPGEKGIKGDRGENGQKGEPGIGFRGPVGQAGPPGFKGEPGAPGPPGAQGVQGIRGNAGTPGSQGERGAPGLPGMPGQKGERGKRGRNGFAGPSGPSGSPGKEGISGTPGPKGNKGEIGVGAAGPRGPRGLPGPRGDEGIVGVRGPTGMTGLKGFPGVKGERGDRGLLGPKGERGEPMTIFGPQGYKGSKGDPGERGLPGFDGDKGEKGEDGPIGEKGVKGEAGSKGVMGLFGTRGPVGQKGEPGEPGLPGSAGAAGLNGKNGNKGAKGDRGLQGQKGKPGGKGDPGATGDIGQKGTKGLRGLPGRIGAPGAEGMKGEIGSPGKPGIPGSDGPHGQKGEKGTSGDDGASGIPGEKGEKGAKGVPGLGGFKGQMGWPGRTGVAGPDGPQGPQGEPGPQGPRGKRGRPQLCLRGPPGMDGRKGEMGENGPAGQKGEKGDPGLSEEEVKEVVRSEMRGRCDCGGELGRTLMERGLEATEINRQLQGFQKFQLWERTRRDGESQEEMLNKTQGMTMLMTMVKPVMEPPSLIAAAPRAQQITGPSQRKRREAHSQLPAPCLQPMDEGSCQHYALLWYYHLEANTCRPFIFGGCRGNSNRFETKWKCEQKCKTSAARGR

Target ORF information:

RefSeq Version	XM_014961019.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax collagen alpha-1(VII) chain-like (LOC106899270), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014961019.1

ORF Insert Sequence:

ATGTTTTGTG CCCAGATCCT TGCCCTGGCA GTGGCTGTGG TGCAGCACAG AGCTGTGGCC 
GTTCATGAAG CATGCAGGAC GGCGGAGGTG GCCGACATTC TGTTCCTCGT GGGACAGTCA 
CAGGGCACAG GAAGGGAGAG CTCCTGGCTG GTCAAGGACT TCATCAGCAG CATGGCTCGT 
TCCTTTGAGA ACGTGGTGAT GGGCAAAGGA GGCATCCGGC TGGCAGTGGC ACTCTACGGG 
GAGAAACCAA GGATGAGTAT TGAGCTTACG GATTACGTGA CCATCGAAGA AATGTTGGTG 
GCAATTCAGG ATCTCTCCTT CAAAGGTGGA AGCTTAAAAA CAGGGTCAGC CTTGGCCTTT 
GCAGCTCGTG CCATGTCTCG TCTGGACACA CTGCGAGAGG ATGCAGCAAA GGTGGTTGTT 
TTGATCACGG ATGGAAAATC CAGCAACTCA GTTGAAGATG AAGCCCGGGT CCTGCAGGAT 
TTGGGGGTGA CGGTGTTTGC CGTAGGGATT AAGGGTGCTG ACAAGAATGA ATTGAACACA 
ATAGCTTCAG AGCCCACTGC AGAGCATGTG CTTTATGTTG AAGATTTCCA CCTCCTCCAT 
AACGTGGCCC CCAAACTCTC TCGAAGGCTC TGTTTCACTG CCTCGGAGCC ACCACGACCC 
ATCAAACAGA CTGTGCAAGC TGAGAAGATC GTCGGCCCCC GGGATTTGTA TGTCAGTGAG 
CACAGCCACA GCACGCTGAG ACTCACCTGG ATGCCAGCCA CGGGGAAGGT GACGGGCTAC 
CATGTCCATC TGCATCCCTT GCTGCCTTCA GGGCAGCCCG TTTCAGAAGA CCAGCAGCAG 
ATTGTGGTGG ACGGTGACAA AAGTACCGTG CTGGTGACTG ATCTGAAACC CAACACCAAG 
TATGTCTTCA CGGTGAGGGC CATCTATGCA GATACTGTTG GGGAGTCAGC GGTTGTCAAG 
GGGAAAACAA CACCTGTGCC TCCAGTCACT AATTTCCGTG TGATAGAAGA AGGACTTTTT 
AGCTTGAAGG TGGCTTGGAC ACCTCCGCTT GGCAAGCTGG AGGGCTACAA GATCTACATC 
CCCAGAGCCA ACAGACCAGG AATGACCTAC GAGCAGGTTC TGCGGGGTGA TGTCTCATCC 
CACGTGCTGG AGAACTTGCA GGAGGACAGA GAATACAGCA TCAGCATCTA CGCCGTGTAC 
CCCCAGGGCC CCAGCCAGCC TGTGGCTGCT GTGGGGAGAA CATTGAAGCT CCTGCCCGTG 
AAAAGCATCT TGCTGCAGAA TGAGACCACC AACACGCTCC AGGCAAGGTG GACACCCATC 
CGGGGAGCAA CGGGGTACAG GCTCACCTGG ACATCAGCAG AGGGCAGCAT CCAAGATGTG 
AACCTCAGCA ACACCTACAG CTACTACATG ATCCAGGGGC TGCAGCCTGG CACGGAGTAC 
ACTGTTACCA TCAACCCCAT CTTTGGGGAC GTCGAGGGAC CAGTGGTGAG CAGCAAAGCA 
ACGACGGTGG CATCGAGCAC CGTCCAGATG CTGAAAGTGA CTGAGATATC TACCAACAGT 
GCTCTGGTCT CCTGGGACTC AGTTGCTGGG GCCACTGGGT ATCGAGTGGC TTGGGGTCCT 
ACACCAGAGT TCTTTGGCAA AGACCGTCCT CGCCAGCTGG CCCTCAACAG GTCAATCACA 
GCCTACCAGC TCCGCAACCT GGCCCATGAC ACCGAGTACG TGATTTCCCT CTACGTGCTC 
TTTGGCTCCG TGGAAGGACC AGGGATCACA ACCTCAGCCA GGACATCTCC TCTTGGCTAC 
GTCTCCAATT TCAAGGTGAT GTCCTACACC AGCACAAGCC TGTCTCTGGT GTGGAGTGCC 
ACAGCAGCTG CCACCAAATT CAAAGTCACC TGGAGCCCTG TGGGAGCAGA AAAAGAAAAA 
CAGGTTGCCA AGACTCAGTA CCTGGGCAGT AGGGTGCTGG CTTACCAGAT TGACCAGTTA 
CTGCCCAACA CCCTGTATAA AGTCAGCGTT CGGGCTGTCT TCAACAAGAC TGAGGGACCA 
GAGGTGACTC TGACTCACCG CACAGCGCCT CTTGCAGACT CCAACCCCAT CCAGGCCGTT 
GAAGATCTGA GGATTACTGA CACCGGCGTA AATAGCTTGA AGCTGTCTTG GAGGAGACTT 
CCTGGGGTAA CTCATTATAA GATATCCTGG GCGCCGTTCA ATGGTGGCTT GGAAACCTCC 
CAGGTGGTTG CAGCAGAGAC GGTCTCCTTC ACGATCTCCA ACCTGAAGGA GAGCACCACC 
TACACCATCT GCATGTCTGC AATGATCGGG GGACAGGAGG GAAGCCCCAC ACTCCTCACA 
GCCAAAACTT TGGATTTACC CAAAGTGACT GAGTTCATGG TGCAGGAGGC TGCGGAGACC 
AGCGTTTTGC TGACATGGAC AGCTGTTCCT GGGGCATCCA CTTATCTATT AACATGGCGC 
CTGTCCTCAG CTTCTGATCT TTCCCAGGAA CTGCTGTCAG CAGCTTCTAG ATCGTATCGA 
GTGACAGGAC TGAGCACCAA AGAAATGTAT GTGTTTTCCA TAAGACCAGT CTTTGGAGAT 
ACGGAGGGAC CAGAGACAAC ACTCCTTGGG CAAACAGCGG GACCTCACAG AGAGTCACCC 
ACACCCATCA CTCCAGTGAC TTCTACAAAA CTGGGTACGA TGAGATTCCC ATCCTTATCT 
CCTAGCAAGG TGGGGACCAC TCCTCTTCCA CTGGCCACCA CCACAACAGT ACCACCAACT 
CCAGCAACCC TACTGTCGAC CACTGCAGTG TCCACACTGC TGACAACTCT CCCTGGGCCA 
ATCTGTGGCA AGTTCAAAGC AGACATTGGG TTCCTGGTGG ACGAGTCCTC GAGTATTGGC 
CAGAGCAATT TCAACAAAGT GAAGGATTTC CTCTTCCGGA TCATTTCCTA TTTCCCGAAG 
ATTGGGCCTG AAGGGACACA GGTTGCTGTT GCTCAGTACA GCGAGGAGCC CAGGGCAGCC 
TTCTACTTCA ACCAGCACCG GGACCGCAAC AGTGCCCTGA AAGCCGTCAA GGGACTGAGC 
TACGCTGGGG GCAACACAAA AACAGGTCGT GGCATAACCT ACATGCTCAA GGAGTTATTT 
CAGTCCTCCA GAGGGATGAG GCCAGATTTC CCTCACGTGC TGGTGCTGGT GACCGATGGG 
CGATCCCAGG ATGATGTGTT GCTACCAGCC AGAGCAGCCC ATGAGTTGGG GATCCGCGTC 
ATTGCTGTGG GTGTCTCAGG AGCTGACCCC GTTGAGCTCA ACAGCATCTT GCTGCAGCAG 
AACCTCCAGA ATGTTTTCTA CGTCAGCACG TTTGATGACT TCCCCCAGAT CCTCCGAGAG 
CTGATAGAGG TCATCTGTTC TGACCCCGAG CCTTCAGGGC CTCAACTACC ATCTGGGGAG 
AGCTTCAGGA GTTCACCAGC TCCTCATGGA GGCTATGACC CATACAGCTT TACTACAAAG 
GGAGAGAAAG GAGAGAGGGG TCTACCTGGG AAGGATGGAA TTCCCGGGCT GCCGGGCAGA 
CCGGGGCGGA CAGGTCCTCC TGGTCCCCCT GGACTCATGG GTCTCCCTGG CATCCAAGGT 
GATACTGGCT CTCCTGGCTA TCCTGGACCA GCGGGGCCAA AAGGAGAGAG AGGCGAACCA 
GGCTATGTCC TGGGAGGAGT AGAGGTGATT CCTGGCCGAA ATGGGCAGCC TGGCCCCCCT 
GGACAGAAAG GGCAGCCGGG GGTTCCTGGG GTAGCAGGAC CACCAGGTTT ACCAGGGCCA 
CAGGGGCCAC CAGGCATCTC CATCAAGGGT GAACGTGGGG ATTCAGGTAC CAGGGGCCCT 
CGTGGGAGGA ATGGCCTCAA GGGTGACAGA GGAGGAATGG GAGAGCCGGG GAAACCTGGG 
TTGCCAGGCC CAGTAGGGCT AGATGGTGTT CCTGGATTGC CTGGACCAAG GGGAGAAAAG 
GGTGTGATGG GACCTCCAGG AGCACCAGGA CCAAAGGGTA ACAAAGGGCA AGGTGGTGTG 
AAAGGGGAAA AGGGACTCCC AGGACCTCCA GGGCAGAAGG GAGACCAGGG GAACCCAGGA 
TTTCCAGGTG CTCCAGCTAT GGGGGTTTTG GGACCTCCAG GCAAGAAAGG TGTCAGGGGT 
GATATTGGAC CAACAGGGGC CCTGGGACCA CAAGGAGACA AAGGAATACA AGGAGACAAA 
GGAGAGAAGG GAAGCCCAGG TTTCGGGATT CCTGGTCAAC CTGGGCTGAA GGGAGATCCA 
GGCGACAGGG GAAATGTAGG TTTGTCGGGC AAACCAGGTC AGAAGGGGGA TAGAGGTCTG 
AAGGGAGAGA AAGGTGAACC AGGACTACCT GGGAAACCAG GCGAGACCGG ACTAAGAGGT 
AAAGATGGAG AACCAGGGGA GAAAGGCACC TCTGGGACCA AGGGAGAAGC TGGTGTCCCT 
GGAGAACCAG GAGAGAGAGG AATCAGGGGG CCACCTGGAC TCCCAGGACG GCCTGGAGAG 
CAAGGGATAA AAGGCGATAC AGGACCACCT GGAAAGGATG GAATCACTGG AGAGAAGGGA 
GACAAGGGCG AAGCTGGTGA GCCTGGGCTG CCTGGGACTC CTGGAAGCCT TGTGTCTTCA 
CTGGAGAGCA CCATCACCCT GAAAGGCGAC AAGGGGGATC GTGGGAAGGA TGGCTTGAAA 
GGCGAAAGAG GTCCTCCAGG CCCAAAGGGA GAACCCGGTC CACCAGGCAA AGGAGTGGAA 
ATGAAGGATC TGGAGAGGCT TTTTGAAGCA AATGGTATCA AGCTGGCACT GCTGAAGGAC 
CTCTCCAATG CACTCCTGCA GGATGGGCTG GATGGGCTGG TGTGGCAGCT GGGTGGCTCC 
AGGACAAGCA AGACCTCCAG GAGAAAGCAA GCAGTTCTTC ATGCTACTGA GCATGGCGAT 
TCACTGGTGT TTGATACTTC TCCTGGTGCC CTGGCCAGCA CTGAGGTGAC AGAAGAAGCA 
CAGAGCCTGG AGGTGGAGGC TCAGTTTAGG GTGCCGGTGT CTGGAGGACT CCTTAAGGGT 
CCATCTGCTG GTGGTTCTGA ACCTGAGTAC CAGCCCCTAG GAAACCCTCC TGAGTCCTTG 
GAGAGAACAT CTGAGGAAGG AAAGGAGGAG AGAGACCCAA GATGGACAAT CACTTCTCTC 
AGTGGTAGCT TGGCTTTGCC CCCACTGCAC CCAGAAGATG ATCTGCAAGG AAACCAGTCC 
ATGGAGCTTC TTGAGTCCCT GGAGGGGAGA GTGGAAGGAC AACCACAGCA GGACACTAGC 
CACGAAAATG AGAATTTAGA CGACGGCTCT GCGCTCCCAA GGCACGTTAG AGCCCAGAGA 
TCTGCTGAGG ACCAGAGACA CGTGGTTCAT CCCCCTGGTG CTCCAGGAGC TCGGAAATCC 
CCATCTGGAT CCACGGCATA CCCAGGCCCT CATCACCCAA GGAGAATTAA GAAGGAGGAA 
GCTGGTCCAC CAGATCCACC ATCCCATGGC CAAAAGGGAG AAATGGGAGC TCCGGGAGTC 
CGTGGAGACA AAGGAGAAAA GGGGCAGCCT GGGGAGATGG GGGAACCTGG AGAAAAGGGC 
ATCAAAGGAG ACAGAGGTGA AAATGGTCAA AAGGGAGAAC CAGGAATTGG GTTCAGAGGC 
CCTGTTGGTC AGGCTGGACC ACCTGGATTT AAAGGTGAGC CAGGGGCTCC AGGGCCTCCA 
GGAGCTCAGG GAGTCCAGGG AATTCGTGGC AATGCCGGCA CCCCCGGGTC ACAAGGGGAA 
AGAGGGGCTC CAGGTCTGCC TGGGATGCCA GGACAAAAGG GAGAGCGTGG GAAGAGAGGA 
CGGAATGGAT TTGCTGGGCC CTCAGGACCA TCAGGATCCC CAGGAAAAGA GGGGATTTCT 
GGGACACCTG GACCCAAAGG CAACAAGGGA GAAATCGGCG TTGGAGCAGC TGGCCCAAGA 
GGTCCTCGTG GACTGCCTGG CCCTCGGGGT GACGAGGGCA TCGTGGGAGT CCGTGGCCCC 
ACCGGCATGA CGGGTCTAAA AGGCTTCCCG GGTGTGAAGG GCGAAAGAGG TGATCGGGGA 
CTTCTGGGAC CCAAAGGAGA AAGAGGTGAG CCCATGACTA TTTTTGGACC CCAAGGCTAT 
AAAGGCAGTA AAGGAGATCC TGGTGAACGG GGCCTGCCAG GTTTTGACGG AGACAAAGGC 
GAGAAAGGAG AGGATGGGCC TATAGGAGAA AAGGGAGTCA AAGGTGAAGC TGGCTCCAAG 
GGAGTGATGG GGCTTTTTGG AACAAGAGGA CCAGTGGGAC AGAAGGGGGA GCCAGGAGAA 
CCAGGCTTGC CGGGCTCTGC TGGTGCAGCA GGTCTTAATG GGAAGAACGG AAACAAGGGA 
GCCAAAGGTG ACAGAGGTCT TCAGGGGCAG AAGGGGAAGC CGGGAGGAAA AGGTGATCCT 
GGGGCCACTG GTGACATTGG ACAGAAAGGA ACAAAGGGCT TACGAGGGCT TCCAGGACGC 
ATCGGTGCTC CTGGAGCTGA AGGCATGAAG GGAGAAATTG GCAGTCCTGG AAAACCAGGA 
ATCCCAGGAT CAGATGGACC ACACGGACAA AAGGGTGAAA AGGGAACATC AGGAGACGAT 
GGTGCCTCGG GGATTCCTGG GGAGAAAGGA GAAAAGGGAG CCAAAGGAGT TCCTGGCTTG 
GGAGGCTTCA AAGGACAAAT GGGATGGCCT GGGAGGACTG GAGTTGCTGG ACCAGATGGC 
CCTCAAGGGC CACAGGGTGA ACCAGGGCCC CAGGGTCCAC GAGGGAAAAG AGGGAGACCT 
CAGCTCTGCC TCAGGGGACC CCCAGGCATG GATGGGAGAA AAGGAGAAAT GGGTGAAAAT 
GGTCCAGCCG GCCAGAAAGG AGAGAAGGGA GATCCTGGTC TTTCTGAGGA AGAGGTGAAG 
GAGGTGGTCA GGAGCGAAAT GAGAGGCAGA TGCGATTGTG GAGGCGAGTT GGGAAGGACA 
CTCATGGAAA GGGGCCTGGA GGCAACTGAA ATCAACAGGC AGCTCCAAGG GTTCCAAAAG 
TTCCAGCTTT GGGAACGCAC CAGAAGGGAT GGTGAAAGCC AGGAAGAAAT GCTGAACAAA 
ACCCAGGGGA TGACCATGTT GATGACAATG GTGAAGCCCG TCATGGAGCC ACCTTCACTT 
ATTGCAGCGG CTCCCCGGGC GCAGCAGATA ACAGGTCCCT CTCAGCGCAA GCGGCGAGAA 
GCCCACAGTC AATTACCAGC CCCCTGCCTG CAGCCGATGG ATGAAGGCTC CTGCCAGCAC 
TATGCCCTGC TCTGGTATTA CCACCTGGAG GCAAACACCT GCCGGCCCTT CATCTTTGGG 
GGATGCCGAG GGAACAGCAA CCGCTTCGAA ACCAAGTGGA AGTGTGAGCA GAAGTGCAAA 
ACCTCAGCAG CCAGAGGGAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC106899270 cDNA ORF clone, Calidris pugnax(ruff)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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