TOP2A cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	TOP2A
Entrez Gene ID	480525
Full Name	DNA topoisomerase II alpha
General protein information	Preferred Names DNA topoisomerase II alpha Names DNA topoisomerase 2-alpha topoisomerase (DNA) II alpha 170kDa
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005624532.2	XP_005624589.1	DNA topoisomerase 2-alpha isoform X1
XM_537646.4	XP_537646.2	DNA topoisomerase 2-alpha isoform X2
XM_537646.5	XP_537646.2	DNA topoisomerase 2-alpha
XM_005624533.2	XP_005624590.1	DNA topoisomerase 2-alpha isoform X3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Top2a	NP_035753.2
Rattus norvegicus (Norway rat)	Top2a	NP_071519.2
Xenopus tropicalis (tropical clawed frog)	topoii	XP_002942632.2
Danio rerio (zebrafish)	top2a	NP_001003834.1
Drosophila melanogaster (fruit fly)	Top2	NP_001260580.1
Caenorhabditis elegans (roundworm)	top-2	NP_496536.1
Bos taurus (cattle)	TOP2A	XP_002696055.2
Gallus gallus (chicken)	TOP2A	NP_990122.1
Homo sapiens (human)	TOP2A	NP_001058.2
Pan troglodytes (chimpanzee)	TOP2A	XP_003315524.1
Canis lupus familiaris (dog)	TOP2A	XP_537646.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008917	XP_319665.4
	TOP2A	XP_002808175.1

Gene Ontology

The following TOP2A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOP2A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe11750	XM_005624532.2 Latest version!	Canis lupus familiaris topoisomerase (DNA) II alpha (TOP2A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe11751	XM_537646.5 Latest version!	Canis lupus familiaris DNA topoisomerase II alpha (TOP2A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe11752	XM_005624533.2 Latest version!	Canis lupus familiaris topoisomerase (DNA) II alpha (TOP2A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
OCe11751	XM_537646.4	Canis lupus familiaris topoisomerase (DNA) II alpha (TOP2A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TOP2A ( XM_005624532.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005624589.1 Canis lupus familiaris topoisomerase (DNA) II alpha (TOP2A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe11750
Clone ID Related Accession (Same CDS sequence)	XM_005624532.2
Accession Version	XM_005624532.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	DNA topoisomerase 2-alpha isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624532.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTAG TAGTGAAATA TTACCGGCTA CACTGGCTTC TCTGGAGTCC GGTAAATGAA 
AACATGCAAA TCAGCAAAAC AAAGAAAAAT GAAGATGCTA AGAAAAGACT GTCTATTGAA 
AGGATCTATC AAAAGAAAAC CCAACTGGAA CATATTTTGC TCCGCCCGGA CACCTACATT 
GGCTCTGTGG AATTAGTGAC CCAGCAAATG TGGGTTTACG ATGAAGATAT TGGCATGAAC 
TATAGGGAAG TCACCTTTGT CCCTGGGTTG TACAAAATCT TTGATGAGAT TCTGGTCAAT 
GCTGCAGACA ACAAACAAAG GGACCCAAAA ATGTCTTGTA TTAGAGTCAC AATTGACCCG 
GAAAACAATT TAATTAGTAT ATGGAATAAT GGGAAAGGTA TTCCTGTTGT TGAACACAAA 
GTTGAGAAGA TGTATGTCCC AGCACTCATC TTTGGACAGC TCCTAACTTC TAGTAACTAT 
GATGATGATG AAAAGAAAGT AACAGGTGGT CGAAATGGCT ATGGGGCCAA ATTGTGTAAC 
ATATTCAGTA CCAAGTTTAC TGTGGAAACA GCCAGTAGAG AATACAAGAA AATGTTCAAA 
CAGACGTGGA TGGATAACAT GGGAAGGGCT GGTGAGATGG AACTCAGGCC ATTTAATGGG 
GAAGATTATA CATGTATTAC CTTCCAGCCT GACTTGTCCA AGTTTAAAAT GCAAAGCCTA 
GATAAAGACA TTGTTGCACT GATGGTTAGA AGAGCATACG ATATTGCTGG ATCCACTAAA 
GATGTCAAAG TCTTTCTCAA TGGAAATAAA CTACCAGTAA AAGGATTCCG TAGCTACGTA 
GATATGTATC TGAAGGATAA GGTAGATGAA ACTGGCAATC CATTGAAAAT TATCCATGAA 
CAAGTAAACC CCAGGTGGGA AGTATGTTTA ACAATGAGTG AAAAAGGCTT TCAGCAAATT 
AGCTTCGTCA ACAGCATTGC TACCTCCAAG GGTGGCAGAC ATGTTGATTA TATAGCTGAT 
CAGATTGTGA CTAAACTTGT TGATGTTGTG AAGAAGAAGA ACAAGGGTGG TGTTGCAGTA 
AAAGCACATC AGGTGAAAAA TCACATGTGG ATTTTTGTGA ATGCCTTGAT TGAAAACCCA 
ACCTTTGACT CTCAGACGAA AGAAAACATG ACTTTACAAG TCAAGAGCTT TGGATCAACA 
TGCCAATTAA GTGAAAAATT CATCAAAGCT GCAACTGGCT GTGGTATTGT GGAAAGCATT 
CTAAACTGGG TAAAGTTTAA GGCCCAAATC CAATTAAACA AGAAGTGTTC AGCTGTAAAG 
CATAACAGAA TCAAGGGAAT TCCCAAACTT GATGATGCCA ATGACGCAGG GGGTCGAAAC 
TCCAATGAGT GTACACTTAT CCTGACTGAA GGAGACTCAG CTAAAACTTT GGCTGTTTCA 
GGCCTTGGTG TGGTTGGGAG AGACAAATAT GGAGTTTTCC CACTTAGAGG AAAAATACTC 
AATGTTCGAG AAGCTTCTCA TAAACAGATC ATGGAAAATG CTGAAATTAA TAATATCATC 
AAGATCGTGG GTCTTCAGTA CAAGAAAAAC TATGACGATG AAGATTCATT GAAGACTCTT 
CGTTATGGGA AGATAATGAT TATGACAGAT CAGGACCAAG ATGGTTCCCA TATCAAAGGC 
TTGCTGATTA ATTTTATCCA TCATAACTGG CCCTCTCTTC TACGGCATCG TTTTCTGGAG 
GAATTTATCA CTCCGATTGT AAAGGTGTCT AAAAACAAAC AAGAAATGGC ATTCTATAGC 
CTTCCTGAAT TTGAAGAATG GAAGAGTTCT ACTCCAAATC ATAAAAAATG GAAAGTCAAG 
TACTACAAAG GTTTGGGTAC CAGCACATCA AAAGAAGCTA AAGAATACTT TGCAAATATG 
AAAAGACATC GTATTCAGTT CAAATACTCC GGTCCTGAAG ATGATGCTGC CATCAGCTTG 
GCCTTTAGCA AGAAACAGAT AGATGATCGA AAGGAATGGT TAACTCATTT CATGGAAGAT 
AGAAGACAAC GGAAGTTACT TGGCCTTCCT GAGGATTACT TGTATGGGCA AACTACCACA 
TATCTGACAT ACAATGACTT TATAAATAAG GAACTTATCC TGTTCTCAAA TTCTGATAAC 
GAGAGATCTA TCCCATCTAT GGTGGATGGC TTGAAACCAG GTCAAAGAAA GGTTTTGTTT 
ACCTGTTTCA AACGGAATGA CAAGCGAGAG GTGAAGGTTG CCCAGTTGGC TGGGTCAGTG 
GCTGAGATGT CCTCTTATCA TCATGGTGAG ATGTCACTAA TGATGACCAT TATAAATTTG 
GCTCAGAATT TTGTGGGTAG CAATAATCTG AACCTCTTGC AGCCCATTGG TCAGTTTGGT 
ACTAGGCTGC ACGGTGGCAA GGATTCTGCT AGTCCTCGAT ACATCTTCAC AATGCTCAGC 
CCTTTGGCTC GGTTATTGTT TCCACCAAAA GATGATCAGA CTTTAAAGTT TTTATATGAT 
GACAATCAAC GTGTTGAACC TGAATGGTAC ATTCCCATTA TACCCATGGT GCTGATAAAT 
GGTGCTGAAG GAATTGGTAC TGGGTGGTCC TGCAAAATCC CCAACTTTGA TATTCGTGAA 
GTTGTAAATA ATATCAGGCG TTTGATGGAT GGAGAAGAAC CTTTGCCAAT GCTTCCAAGT 
TACAAGAACT TCAGGGGTAC TATTGAAGAG CTGGCTCCAA ATCAGTATGT GATTAGTGGT 
GAAGTGGCTA TACTTAATTC CACAACCATT GAAATCTCAG AGCTTCCTGT CAGAACATGG 
ACCCAGACCT ATAAAGAACA GGTTCTAGAA CCCATGTTGA ATGGCACTGA GAAGACACCA 
CCTCTCATAA CAGATTATAG GGAGTACCAT ACAGACACCA CTGTGAAGTT TGTTGTGAAG 
ATGACTGAAG AGAAACTGGC AGAGGCAGAG AGAGTGGGGC TACACAAAGT CTTCAAACTC 
CAAACTAGTC TCACATGCAA CTCCATGGTG CTTTTTGACC ATGTAGGTTG TTTAAAGAAA 
TATGACACAG TGTTGGATAT TCTAAGAGAC TTCTTTGAAC TAAGGCTTAA ATATTATGGA 
TTAAGAAAAG AATGGCTTCT AGGAATGCTT GGCGCTGAAT CTGCTAAACT GAACAATCAG 
GCTCGCTTTA TCTTAGAGAA AATAGATGGC AAAATAATTA TTGAAAATAA GCCTAAGAAA 
GAATTAATTA AAGTTCTGAT TCAGAGGGGA TATGATTCGG ATCCTGTGAA GGCTTGGAAA 
GAAGCTCAGC AAAAGGTACC AGATGAAGAA GAGAATGAAG AGAGTGACAA TGAAAATGAA 
GCTGAAAAGA GTGACTCTGT AAGAGATTCT GGACCAACCT TCAACTACCT TCTTGATATG 
CCTCTTTGGT ATCTCACCAA GGAGAAGAAG GATGAACTGT GCAAGCTGAG AAATGAAAAA 
GAGCAAGAAC TAGAAACATT AAAGAGAAAG AGTCCAACAG ATTTGTGGAA AGAAGACTTG 
GCTACTTTTG TTGAAGAGCT AGAGGCTGTT GAAACAAAGG AAAAACAAGA AGAACAAATA 
GGACTTCCAG GGAAAGGAGG GAAAGGAAAG GGGAAAAAAA CACAAATGGC TGAAGTTTTG 
CCTTCTCCTT GCGGAAAAAG AGTCATCCCC CGAGTGACCA TAGAGATGAA AGCAGAGGCA 
GAAAAGAAAA ATAAAAAGAA AATTAAGAAT GAAAACACTG AAGGGAGCCC TCAAGAAGAG 
AGTATCGAAA TAGAAGGTCT GAAACAAAGG TTGGAGAAGA AACAGAAAAG AGAACCAGGT 
ACCAAGACAA AGAAGCAAAC TACATTGCCG TTTAAACCCA TCAAAAAAGG AAAGAAGAGA 
AACCCCTGGT CTGATTCAGA ATCAGATATG AGCAGTAATG AAAGTAACTT TGATGTCCCT 
CCACGAGAGG TAGAGCCACG GAGAGCAGCA ACAAAAACCA AATTCACAGT GGATTTGGAT 
TTAGACGACA GTTTCTCAGA TTTTGAAGAA AGAATTCAAG ATGAGGATTC TGTTCCATCA 
GATGTCAGCC CACATAAGAC CAAAGCTTCC CCAAAACGTA CTGCCAAAGA ACTGAAGCCA 
CAGAAAAGTG CCCCCCCAGT AATGGACCTT GATGCTGATG ATGCCAAGGA CAGTGTACCA 
CTTTCTCCAG GTTCTACTGC TGATTTCTTG GCTGAAACTG AAATTGTAAA CCCCCTTCCT 
AAAAAGAAGG TGGACGTGAA GAAGATAGCA CCAAAAAGTC AGTCTTCCAC CTCCACTACA 
GGTGCAAAAA AAAGGGCTGC ACCAAAAGGA ACCAAAAAGG ATCCAGGCTT AAATTCTGAT 
GTCTCTCAAA AGCCTGATCC CCCAAAAACC AAAAATCACC GCAAAAGGAA GCCATCCACT 
TCTGATGATT CCGACTCTAA TTTTGAGAAG ATGATTTCTA AAGCAGCCAC AAACAAGAAA 
TCCAAGGGGG AGAGTGAAGA CTTCCATCTG GACCTAGACT CAGGTGTGGC TCCTCGGGCC 
AAATCTGGAC GTGCAAGGAA ACCTATAAAG TACCTTGAAG AATCAGATGA AGATGATCTG 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624589.1
CDS	103..4728
Translation	MVLVVKYYRLHWLLWSPVNENMQISKTKKNEDAKKRLSIERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDIGMNYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGEMELRPFNGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNKLPVKGFRSYVDMYLKDKVDETGNPLKIIHEQVNPRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYIADQIVTKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQVKSFGSTCQLSEKFIKAATGCGIVESILNWVKFKAQIQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSNECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYDDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEMAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFANMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTHFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPKDDQTLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDIREVVNNIRRLMDGEEPLPMLPSYKNFRGTIEELAPNQYVISGEVAILNSTTIEISELPVRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMTEEKLAEAERVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNENEAEKSDSVRDSGPTFNYLLDMPLWYLTKEKKDELCKLRNEKEQELETLKRKSPTDLWKEDLATFVEELEAVETKEKQEEQIGLPGKGGKGKGKKTQMAEVLPSPCGKRVIPRVTIEMKAEAEKKNKKKIKNENTEGSPQEESIEIEGLKQRLEKKQKREPGTKTKKQTTLPFKPIKKGKKRNPWSDSESDMSSNESNFDVPPREVEPRRAATKTKFTVDLDLDDSFSDFEERIQDEDSVPSDVSPHKTKASPKRTAKELKPQKSAPPVMDLDADDAKDSVPLSPGSTADFLAETEIVNPLPKKKVDVKKIAPKSQSSTSTTGAKKRAAPKGTKKDPGLNSDVSQKPDPPKTKNHRKRKPSTSDDSDSNFEKMISKAATNKKSKGESEDFHLDLDSGVAPRAKSGRARKPIKYLEESDEDDLF

Target ORF information:

RefSeq Version	XM_005624532.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris topoisomerase (DNA) II alpha (TOP2A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624532.2

ORF Insert Sequence:

ATGGTGCTAG TAGTGAAATA TTACCGGCTA CACTGGCTTC TCTGGAGTCC GGTAAATGAA 
AACATGCAAA TCAGCAAAAC AAAGAAAAAT GAAGATGCTA AGAAAAGACT GTCTATTGAA 
AGGATCTATC AAAAGAAAAC CCAACTGGAA CATATTTTGC TCCGCCCGGA CACCTACATT 
GGCTCTGTGG AATTAGTGAC CCAGCAAATG TGGGTTTACG ATGAAGATAT TGGCATGAAC 
TATAGGGAAG TCACCTTTGT CCCTGGGTTG TACAAAATCT TTGATGAGAT TCTGGTCAAT 
GCTGCAGACA ACAAACAAAG GGACCCAAAA ATGTCTTGTA TTAGAGTCAC AATTGACCCG 
GAAAACAATT TAATTAGTAT ATGGAATAAT GGGAAAGGTA TTCCTGTTGT TGAACACAAA 
GTTGAGAAGA TGTATGTCCC AGCACTCATC TTTGGACAGC TCCTAACTTC TAGTAACTAT 
GATGATGATG AAAAGAAAGT AACAGGTGGT CGAAATGGCT ATGGGGCCAA ATTGTGTAAC 
ATATTCAGTA CCAAGTTTAC TGTGGAAACA GCCAGTAGAG AATACAAGAA AATGTTCAAA 
CAGACGTGGA TGGATAACAT GGGAAGGGCT GGTGAGATGG AACTCAGGCC ATTTAATGGG 
GAAGATTATA CATGTATTAC CTTCCAGCCT GACTTGTCCA AGTTTAAAAT GCAAAGCCTA 
GATAAAGACA TTGTTGCACT GATGGTTAGA AGAGCATACG ATATTGCTGG ATCCACTAAA 
GATGTCAAAG TCTTTCTCAA TGGAAATAAA CTACCAGTAA AAGGATTCCG TAGCTACGTA 
GATATGTATC TGAAGGATAA GGTAGATGAA ACTGGCAATC CATTGAAAAT TATCCATGAA 
CAAGTAAACC CCAGGTGGGA AGTATGTTTA ACAATGAGTG AAAAAGGCTT TCAGCAAATT 
AGCTTCGTCA ACAGCATTGC TACCTCCAAG GGTGGCAGAC ATGTTGATTA TATAGCTGAT 
CAGATTGTGA CTAAACTTGT TGATGTTGTG AAGAAGAAGA ACAAGGGTGG TGTTGCAGTA 
AAAGCACATC AGGTGAAAAA TCACATGTGG ATTTTTGTGA ATGCCTTGAT TGAAAACCCA 
ACCTTTGACT CTCAGACGAA AGAAAACATG ACTTTACAAG TCAAGAGCTT TGGATCAACA 
TGCCAATTAA GTGAAAAATT CATCAAAGCT GCAACTGGCT GTGGTATTGT GGAAAGCATT 
CTAAACTGGG TAAAGTTTAA GGCCCAAATC CAATTAAACA AGAAGTGTTC AGCTGTAAAG 
CATAACAGAA TCAAGGGAAT TCCCAAACTT GATGATGCCA ATGACGCAGG GGGTCGAAAC 
TCCAATGAGT GTACACTTAT CCTGACTGAA GGAGACTCAG CTAAAACTTT GGCTGTTTCA 
GGCCTTGGTG TGGTTGGGAG AGACAAATAT GGAGTTTTCC CACTTAGAGG AAAAATACTC 
AATGTTCGAG AAGCTTCTCA TAAACAGATC ATGGAAAATG CTGAAATTAA TAATATCATC 
AAGATCGTGG GTCTTCAGTA CAAGAAAAAC TATGACGATG AAGATTCATT GAAGACTCTT 
CGTTATGGGA AGATAATGAT TATGACAGAT CAGGACCAAG ATGGTTCCCA TATCAAAGGC 
TTGCTGATTA ATTTTATCCA TCATAACTGG CCCTCTCTTC TACGGCATCG TTTTCTGGAG 
GAATTTATCA CTCCGATTGT AAAGGTGTCT AAAAACAAAC AAGAAATGGC ATTCTATAGC 
CTTCCTGAAT TTGAAGAATG GAAGAGTTCT ACTCCAAATC ATAAAAAATG GAAAGTCAAG 
TACTACAAAG GTTTGGGTAC CAGCACATCA AAAGAAGCTA AAGAATACTT TGCAAATATG 
AAAAGACATC GTATTCAGTT CAAATACTCC GGTCCTGAAG ATGATGCTGC CATCAGCTTG 
GCCTTTAGCA AGAAACAGAT AGATGATCGA AAGGAATGGT TAACTCATTT CATGGAAGAT 
AGAAGACAAC GGAAGTTACT TGGCCTTCCT GAGGATTACT TGTATGGGCA AACTACCACA 
TATCTGACAT ACAATGACTT TATAAATAAG GAACTTATCC TGTTCTCAAA TTCTGATAAC 
GAGAGATCTA TCCCATCTAT GGTGGATGGC TTGAAACCAG GTCAAAGAAA GGTTTTGTTT 
ACCTGTTTCA AACGGAATGA CAAGCGAGAG GTGAAGGTTG CCCAGTTGGC TGGGTCAGTG 
GCTGAGATGT CCTCTTATCA TCATGGTGAG ATGTCACTAA TGATGACCAT TATAAATTTG 
GCTCAGAATT TTGTGGGTAG CAATAATCTG AACCTCTTGC AGCCCATTGG TCAGTTTGGT 
ACTAGGCTGC ACGGTGGCAA GGATTCTGCT AGTCCTCGAT ACATCTTCAC AATGCTCAGC 
CCTTTGGCTC GGTTATTGTT TCCACCAAAA GATGATCAGA CTTTAAAGTT TTTATATGAT 
GACAATCAAC GTGTTGAACC TGAATGGTAC ATTCCCATTA TACCCATGGT GCTGATAAAT 
GGTGCTGAAG GAATTGGTAC TGGGTGGTCC TGCAAAATCC CCAACTTTGA TATTCGTGAA 
GTTGTAAATA ATATCAGGCG TTTGATGGAT GGAGAAGAAC CTTTGCCAAT GCTTCCAAGT 
TACAAGAACT TCAGGGGTAC TATTGAAGAG CTGGCTCCAA ATCAGTATGT GATTAGTGGT 
GAAGTGGCTA TACTTAATTC CACAACCATT GAAATCTCAG AGCTTCCTGT CAGAACATGG 
ACCCAGACCT ATAAAGAACA GGTTCTAGAA CCCATGTTGA ATGGCACTGA GAAGACACCA 
CCTCTCATAA CAGATTATAG GGAGTACCAT ACAGACACCA CTGTGAAGTT TGTTGTGAAG 
ATGACTGAAG AGAAACTGGC AGAGGCAGAG AGAGTGGGGC TACACAAAGT CTTCAAACTC 
CAAACTAGTC TCACATGCAA CTCCATGGTG CTTTTTGACC ATGTAGGTTG TTTAAAGAAA 
TATGACACAG TGTTGGATAT TCTAAGAGAC TTCTTTGAAC TAAGGCTTAA ATATTATGGA 
TTAAGAAAAG AATGGCTTCT AGGAATGCTT GGCGCTGAAT CTGCTAAACT GAACAATCAG 
GCTCGCTTTA TCTTAGAGAA AATAGATGGC AAAATAATTA TTGAAAATAA GCCTAAGAAA 
GAATTAATTA AAGTTCTGAT TCAGAGGGGA TATGATTCGG ATCCTGTGAA GGCTTGGAAA 
GAAGCTCAGC AAAAGGTACC AGATGAAGAA GAGAATGAAG AGAGTGACAA TGAAAATGAA 
GCTGAAAAGA GTGACTCTGT AAGAGATTCT GGACCAACCT TCAACTACCT TCTTGATATG 
CCTCTTTGGT ATCTCACCAA GGAGAAGAAG GATGAACTGT GCAAGCTGAG AAATGAAAAA 
GAGCAAGAAC TAGAAACATT AAAGAGAAAG AGTCCAACAG ATTTGTGGAA AGAAGACTTG 
GCTACTTTTG TTGAAGAGCT AGAGGCTGTT GAAACAAAGG AAAAACAAGA AGAACAAATA 
GGACTTCCAG GGAAAGGAGG GAAAGGAAAG GGGAAAAAAA CACAAATGGC TGAAGTTTTG 
CCTTCTCCTT GCGGAAAAAG AGTCATCCCC CGAGTGACCA TAGAGATGAA AGCAGAGGCA 
GAAAAGAAAA ATAAAAAGAA AATTAAGAAT GAAAACACTG AAGGGAGCCC TCAAGAAGAG 
AGTATCGAAA TAGAAGGTCT GAAACAAAGG TTGGAGAAGA AACAGAAAAG AGAACCAGGT 
ACCAAGACAA AGAAGCAAAC TACATTGCCG TTTAAACCCA TCAAAAAAGG AAAGAAGAGA 
AACCCCTGGT CTGATTCAGA ATCAGATATG AGCAGTAATG AAAGTAACTT TGATGTCCCT 
CCACGAGAGG TAGAGCCACG GAGAGCAGCA ACAAAAACCA AATTCACAGT GGATTTGGAT 
TTAGACGACA GTTTCTCAGA TTTTGAAGAA AGAATTCAAG ATGAGGATTC TGTTCCATCA 
GATGTCAGCC CACATAAGAC CAAAGCTTCC CCAAAACGTA CTGCCAAAGA ACTGAAGCCA 
CAGAAAAGTG CCCCCCCAGT AATGGACCTT GATGCTGATG ATGCCAAGGA CAGTGTACCA 
CTTTCTCCAG GTTCTACTGC TGATTTCTTG GCTGAAACTG AAATTGTAAA CCCCCTTCCT 
AAAAAGAAGG TGGACGTGAA GAAGATAGCA CCAAAAAGTC AGTCTTCCAC CTCCACTACA 
GGTGCAAAAA AAAGGGCTGC ACCAAAAGGA ACCAAAAAGG ATCCAGGCTT AAATTCTGAT 
GTCTCTCAAA AGCCTGATCC CCCAAAAACC AAAAATCACC GCAAAAGGAA GCCATCCACT 
TCTGATGATT CCGACTCTAA TTTTGAGAAG ATGATTTCTA AAGCAGCCAC AAACAAGAAA 
TCCAAGGGGG AGAGTGAAGA CTTCCATCTG GACCTAGACT CAGGTGTGGC TCCTCGGGCC 
AAATCTGGAC GTGCAAGGAA ACCTATAAAG TACCTTGAAG AATCAGATGA AGATGATCTG 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP2A ( XM_537646.4 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_537646.2 Canis lupus familiaris topoisomerase (DNA) II alpha (TOP2A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe11751
Clone ID Related Accession (Same CDS sequence)	XM_537646.4 , XM_537646.5
Accession Version	XM_537646.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	DNA topoisomerase 2-alpha isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_537646.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGT CACCACTGCA GCCGGTAAAT GAAAACATGC AAATCAGCAA AACAAAGAAA 
AATGAAGATG CTAAGAAAAG ACTGTCTATT GAAAGGATCT ATCAAAAGAA AACCCAACTG 
GAACATATTT TGCTCCGCCC GGACACCTAC ATTGGCTCTG TGGAATTAGT GACCCAGCAA 
ATGTGGGTTT ACGATGAAGA TATTGGCATG AACTATAGGG AAGTCACCTT TGTCCCTGGG 
TTGTACAAAA TCTTTGATGA GATTCTGGTC AATGCTGCAG ACAACAAACA AAGGGACCCA 
AAAATGTCTT GTATTAGAGT CACAATTGAC CCGGAAAACA ATTTAATTAG TATATGGAAT 
AATGGGAAAG GTATTCCTGT TGTTGAACAC AAAGTTGAGA AGATGTATGT CCCAGCACTC 
ATCTTTGGAC AGCTCCTAAC TTCTAGTAAC TATGATGATG ATGAAAAGAA AGTAACAGGT 
GGTCGAAATG GCTATGGGGC CAAATTGTGT AACATATTCA GTACCAAGTT TACTGTGGAA 
ACAGCCAGTA GAGAATACAA GAAAATGTTC AAACAGACGT GGATGGATAA CATGGGAAGG 
GCTGGTGAGA TGGAACTCAG GCCATTTAAT GGGGAAGATT ATACATGTAT TACCTTCCAG 
CCTGACTTGT CCAAGTTTAA AATGCAAAGC CTAGATAAAG ACATTGTTGC ACTGATGGTT 
AGAAGAGCAT ACGATATTGC TGGATCCACT AAAGATGTCA AAGTCTTTCT CAATGGAAAT 
AAACTACCAG TAAAAGGATT CCGTAGCTAC GTAGATATGT ATCTGAAGGA TAAGGTAGAT 
GAAACTGGCA ATCCATTGAA AATTATCCAT GAACAAGTAA ACCCCAGGTG GGAAGTATGT 
TTAACAATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTCG TCAACAGCAT TGCTACCTCC 
AAGGGTGGCA GACATGTTGA TTATATAGCT GATCAGATTG TGACTAAACT TGTTGATGTT 
GTGAAGAAGA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTGAA AAATCACATG 
TGGATTTTTG TGAATGCCTT GATTGAAAAC CCAACCTTTG ACTCTCAGAC GAAAGAAAAC 
ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAAT TAAGTGAAAA ATTCATCAAA 
GCTGCAACTG GCTGTGGTAT TGTGGAAAGC ATTCTAAACT GGGTAAAGTT TAAGGCCCAA 
ATCCAATTAA ACAAGAAGTG TTCAGCTGTA AAGCATAACA GAATCAAGGG AATTCCCAAA 
CTTGATGATG CCAATGACGC AGGGGGTCGA AACTCCAATG AGTGTACACT TATCCTGACT 
GAAGGAGACT CAGCTAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAA 
TATGGAGTTT TCCCACTTAG AGGAAAAATA CTCAATGTTC GAGAAGCTTC TCATAAACAG 
ATCATGGAAA ATGCTGAAAT TAATAATATC ATCAAGATCG TGGGTCTTCA GTACAAGAAA 
AACTATGACG ATGAAGATTC ATTGAAGACT CTTCGTTATG GGAAGATAAT GATTATGACA 
GATCAGGACC AAGATGGTTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCATAAC 
TGGCCCTCTC TTCTACGGCA TCGTTTTCTG GAGGAATTTA TCACTCCGAT TGTAAAGGTG 
TCTAAAAACA AACAAGAAAT GGCATTCTAT AGCCTTCCTG AATTTGAAGA ATGGAAGAGT 
TCTACTCCAA ATCATAAAAA ATGGAAAGTC AAGTACTACA AAGGTTTGGG TACCAGCACA 
TCAAAAGAAG CTAAAGAATA CTTTGCAAAT ATGAAAAGAC ATCGTATTCA GTTCAAATAC 
TCCGGTCCTG AAGATGATGC TGCCATCAGC TTGGCCTTTA GCAAGAAACA GATAGATGAT 
CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGAC AACGGAAGTT ACTTGGCCTT 
CCTGAGGATT ACTTGTATGG GCAAACTACC ACATATCTGA CATACAATGA CTTTATAAAT 
AAGGAACTTA TCCTGTTCTC AAATTCTGAT AACGAGAGAT CTATCCCATC TATGGTGGAT 
GGCTTGAAAC CAGGTCAAAG AAAGGTTTTG TTTACCTGTT TCAAACGGAA TGACAAGCGA 
GAGGTGAAGG TTGCCCAGTT GGCTGGGTCA GTGGCTGAGA TGTCCTCTTA TCATCATGGT 
GAGATGTCAC TAATGATGAC CATTATAAAT TTGGCTCAGA ATTTTGTGGG TAGCAATAAT 
CTGAACCTCT TGCAGCCCAT TGGTCAGTTT GGTACTAGGC TGCACGGTGG CAAGGATTCT 
GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTATT GTTTCCACCA 
AAAGATGATC AGACTTTAAA GTTTTTATAT GATGACAATC AACGTGTTGA ACCTGAATGG 
TACATTCCCA TTATACCCAT GGTGCTGATA AATGGTGCTG AAGGAATTGG TACTGGGTGG 
TCCTGCAAAA TCCCCAACTT TGATATTCGT GAAGTTGTAA ATAATATCAG GCGTTTGATG 
GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAGGGG TACTATTGAA 
GAGCTGGCTC CAAATCAGTA TGTGATTAGT GGTGAAGTGG CTATACTTAA TTCCACAACC 
ATTGAAATCT CAGAGCTTCC TGTCAGAACA TGGACCCAGA CCTATAAAGA ACAGGTTCTA 
GAACCCATGT TGAATGGCAC TGAGAAGACA CCACCTCTCA TAACAGATTA TAGGGAGTAC 
CATACAGACA CCACTGTGAA GTTTGTTGTG AAGATGACTG AAGAGAAACT GGCAGAGGCA 
GAGAGAGTGG GGCTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCCATG 
GTGCTTTTTG ACCATGTAGG TTGTTTAAAG AAATATGACA CAGTGTTGGA TATTCTAAGA 
GACTTCTTTG AACTAAGGCT TAAATATTAT GGATTAAGAA AAGAATGGCT TCTAGGAATG 
CTTGGCGCTG AATCTGCTAA ACTGAACAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT 
GGCAAAATAA TTATTGAAAA TAAGCCTAAG AAAGAATTAA TTAAAGTTCT GATTCAGAGG 
GGATATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCTC AGCAAAAGGT ACCAGATGAA 
GAAGAGAATG AAGAGAGTGA CAATGAAAAT GAAGCTGAAA AGAGTGACTC TGTAAGAGAT 
TCTGGACCAA CCTTCAACTA CCTTCTTGAT ATGCCTCTTT GGTATCTCAC CAAGGAGAAG 
AAGGATGAAC TGTGCAAGCT GAGAAATGAA AAAGAGCAAG AACTAGAAAC ATTAAAGAGA 
AAGAGTCCAA CAGATTTGTG GAAAGAAGAC TTGGCTACTT TTGTTGAAGA GCTAGAGGCT 
GTTGAAACAA AGGAAAAACA AGAAGAACAA ATAGGACTTC CAGGGAAAGG AGGGAAAGGA 
AAGGGGAAAA AAACACAAAT GGCTGAAGTT TTGCCTTCTC CTTGCGGAAA AAGAGTCATC 
CCCCGAGTGA CCATAGAGAT GAAAGCAGAG GCAGAAAAGA AAAATAAAAA GAAAATTAAG 
AATGAAAACA CTGAAGGGAG CCCTCAAGAA GAGAGTATCG AAATAGAAGG TCTGAAACAA 
AGGTTGGAGA AGAAACAGAA AAGAGAACCA GGTACCAAGA CAAAGAAGCA AACTACATTG 
CCGTTTAAAC CCATCAAAAA AGGAAAGAAG AGAAACCCCT GGTCTGATTC AGAATCAGAT 
ATGAGCAGTA ATGAAAGTAA CTTTGATGTC CCTCCACGAG AGGTAGAGCC ACGGAGAGCA 
GCAACAAAAA CCAAATTCAC AGTGGATTTG GATTTAGACG ACAGTTTCTC AGATTTTGAA 
GAAAGAATTC AAGATGAGGA TTCTGTTCCA TCAGATGTCA GCCCACATAA GACCAAAGCT 
TCCCCAAAAC GTACTGCCAA AGAACTGAAG CCACAGAAAA GTGCCCCCCC AGTAATGGAC 
CTTGATGCTG ATGATGCCAA GGACAGTGTA CCACTTTCTC CAGGTTCTAC TGCTGATTTC 
TTGGCTGAAA CTGAAATTGT AAACCCCCTT CCTAAAAAGA AGGTGGACGT GAAGAAGATA 
GCACCAAAAA GTCAGTCTTC CACCTCCACT ACAGGTGCAA AAAAAAGGGC TGCACCAAAA 
GGAACCAAAA AGGATCCAGG CTTAAATTCT GATGTCTCTC AAAAGCCTGA TCCCCCAAAA 
ACCAAAAATC ACCGCAAAAG GAAGCCATCC ACTTCTGATG ATTCCGACTC TAATTTTGAG 
AAGATGATTT CTAAAGCAGC CACAAACAAG AAATCCAAGG GGGAGAGTGA AGACTTCCAT 
CTGGACCTAG ACTCAGGTGT GGCTCCTCGG GCCAAATCTG GACGTGCAAG GAAACCTATA 
AAGTACCTTG AAGAATCAGA TGAAGATGAT CTGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_537646.2
CDS	107..4705
Translation	MEVSPLQPVNENMQISKTKKNEDAKKRLSIERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDIGMNYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGEMELRPFNGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNKLPVKGFRSYVDMYLKDKVDETGNPLKIIHEQVNPRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYIADQIVTKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQVKSFGSTCQLSEKFIKAATGCGIVESILNWVKFKAQIQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSNECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYDDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEMAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFANMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTHFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPKDDQTLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDIREVVNNIRRLMDGEEPLPMLPSYKNFRGTIEELAPNQYVISGEVAILNSTTIEISELPVRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMTEEKLAEAERVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNENEAEKSDSVRDSGPTFNYLLDMPLWYLTKEKKDELCKLRNEKEQELETLKRKSPTDLWKEDLATFVEELEAVETKEKQEEQIGLPGKGGKGKGKKTQMAEVLPSPCGKRVIPRVTIEMKAEAEKKNKKKIKNENTEGSPQEESIEIEGLKQRLEKKQKREPGTKTKKQTTLPFKPIKKGKKRNPWSDSESDMSSNESNFDVPPREVEPRRAATKTKFTVDLDLDDSFSDFEERIQDEDSVPSDVSPHKTKASPKRTAKELKPQKSAPPVMDLDADDAKDSVPLSPGSTADFLAETEIVNPLPKKKVDVKKIAPKSQSSTSTTGAKKRAAPKGTKKDPGLNSDVSQKPDPPKTKNHRKRKPSTSDDSDSNFEKMISKAATNKKSKGESEDFHLDLDSGVAPRAKSGRARKPIKYLEESDEDDLF

Target ORF information:

RefSeq Version	XM_537646.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris topoisomerase (DNA) II alpha (TOP2A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_537646.4

ORF Insert Sequence:

ATGGAGGTGT CACCACTGCA GCCGGTAAAT GAAAACATGC AAATCAGCAA AACAAAGAAA 
AATGAAGATG CTAAGAAAAG ACTGTCTATT GAAAGGATCT ATCAAAAGAA AACCCAACTG 
GAACATATTT TGCTCCGCCC GGACACCTAC ATTGGCTCTG TGGAATTAGT GACCCAGCAA 
ATGTGGGTTT ACGATGAAGA TATTGGCATG AACTATAGGG AAGTCACCTT TGTCCCTGGG 
TTGTACAAAA TCTTTGATGA GATTCTGGTC AATGCTGCAG ACAACAAACA AAGGGACCCA 
AAAATGTCTT GTATTAGAGT CACAATTGAC CCGGAAAACA ATTTAATTAG TATATGGAAT 
AATGGGAAAG GTATTCCTGT TGTTGAACAC AAAGTTGAGA AGATGTATGT CCCAGCACTC 
ATCTTTGGAC AGCTCCTAAC TTCTAGTAAC TATGATGATG ATGAAAAGAA AGTAACAGGT 
GGTCGAAATG GCTATGGGGC CAAATTGTGT AACATATTCA GTACCAAGTT TACTGTGGAA 
ACAGCCAGTA GAGAATACAA GAAAATGTTC AAACAGACGT GGATGGATAA CATGGGAAGG 
GCTGGTGAGA TGGAACTCAG GCCATTTAAT GGGGAAGATT ATACATGTAT TACCTTCCAG 
CCTGACTTGT CCAAGTTTAA AATGCAAAGC CTAGATAAAG ACATTGTTGC ACTGATGGTT 
AGAAGAGCAT ACGATATTGC TGGATCCACT AAAGATGTCA AAGTCTTTCT CAATGGAAAT 
AAACTACCAG TAAAAGGATT CCGTAGCTAC GTAGATATGT ATCTGAAGGA TAAGGTAGAT 
GAAACTGGCA ATCCATTGAA AATTATCCAT GAACAAGTAA ACCCCAGGTG GGAAGTATGT 
TTAACAATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTCG TCAACAGCAT TGCTACCTCC 
AAGGGTGGCA GACATGTTGA TTATATAGCT GATCAGATTG TGACTAAACT TGTTGATGTT 
GTGAAGAAGA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTGAA AAATCACATG 
TGGATTTTTG TGAATGCCTT GATTGAAAAC CCAACCTTTG ACTCTCAGAC GAAAGAAAAC 
ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAAT TAAGTGAAAA ATTCATCAAA 
GCTGCAACTG GCTGTGGTAT TGTGGAAAGC ATTCTAAACT GGGTAAAGTT TAAGGCCCAA 
ATCCAATTAA ACAAGAAGTG TTCAGCTGTA AAGCATAACA GAATCAAGGG AATTCCCAAA 
CTTGATGATG CCAATGACGC AGGGGGTCGA AACTCCAATG AGTGTACACT TATCCTGACT 
GAAGGAGACT CAGCTAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAA 
TATGGAGTTT TCCCACTTAG AGGAAAAATA CTCAATGTTC GAGAAGCTTC TCATAAACAG 
ATCATGGAAA ATGCTGAAAT TAATAATATC ATCAAGATCG TGGGTCTTCA GTACAAGAAA 
AACTATGACG ATGAAGATTC ATTGAAGACT CTTCGTTATG GGAAGATAAT GATTATGACA 
GATCAGGACC AAGATGGTTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCATAAC 
TGGCCCTCTC TTCTACGGCA TCGTTTTCTG GAGGAATTTA TCACTCCGAT TGTAAAGGTG 
TCTAAAAACA AACAAGAAAT GGCATTCTAT AGCCTTCCTG AATTTGAAGA ATGGAAGAGT 
TCTACTCCAA ATCATAAAAA ATGGAAAGTC AAGTACTACA AAGGTTTGGG TACCAGCACA 
TCAAAAGAAG CTAAAGAATA CTTTGCAAAT ATGAAAAGAC ATCGTATTCA GTTCAAATAC 
TCCGGTCCTG AAGATGATGC TGCCATCAGC TTGGCCTTTA GCAAGAAACA GATAGATGAT 
CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGAC AACGGAAGTT ACTTGGCCTT 
CCTGAGGATT ACTTGTATGG GCAAACTACC ACATATCTGA CATACAATGA CTTTATAAAT 
AAGGAACTTA TCCTGTTCTC AAATTCTGAT AACGAGAGAT CTATCCCATC TATGGTGGAT 
GGCTTGAAAC CAGGTCAAAG AAAGGTTTTG TTTACCTGTT TCAAACGGAA TGACAAGCGA 
GAGGTGAAGG TTGCCCAGTT GGCTGGGTCA GTGGCTGAGA TGTCCTCTTA TCATCATGGT 
GAGATGTCAC TAATGATGAC CATTATAAAT TTGGCTCAGA ATTTTGTGGG TAGCAATAAT 
CTGAACCTCT TGCAGCCCAT TGGTCAGTTT GGTACTAGGC TGCACGGTGG CAAGGATTCT 
GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTATT GTTTCCACCA 
AAAGATGATC AGACTTTAAA GTTTTTATAT GATGACAATC AACGTGTTGA ACCTGAATGG 
TACATTCCCA TTATACCCAT GGTGCTGATA AATGGTGCTG AAGGAATTGG TACTGGGTGG 
TCCTGCAAAA TCCCCAACTT TGATATTCGT GAAGTTGTAA ATAATATCAG GCGTTTGATG 
GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAGGGG TACTATTGAA 
GAGCTGGCTC CAAATCAGTA TGTGATTAGT GGTGAAGTGG CTATACTTAA TTCCACAACC 
ATTGAAATCT CAGAGCTTCC TGTCAGAACA TGGACCCAGA CCTATAAAGA ACAGGTTCTA 
GAACCCATGT TGAATGGCAC TGAGAAGACA CCACCTCTCA TAACAGATTA TAGGGAGTAC 
CATACAGACA CCACTGTGAA GTTTGTTGTG AAGATGACTG AAGAGAAACT GGCAGAGGCA 
GAGAGAGTGG GGCTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCCATG 
GTGCTTTTTG ACCATGTAGG TTGTTTAAAG AAATATGACA CAGTGTTGGA TATTCTAAGA 
GACTTCTTTG AACTAAGGCT TAAATATTAT GGATTAAGAA AAGAATGGCT TCTAGGAATG 
CTTGGCGCTG AATCTGCTAA ACTGAACAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT 
GGCAAAATAA TTATTGAAAA TAAGCCTAAG AAAGAATTAA TTAAAGTTCT GATTCAGAGG 
GGATATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCTC AGCAAAAGGT ACCAGATGAA 
GAAGAGAATG AAGAGAGTGA CAATGAAAAT GAAGCTGAAA AGAGTGACTC TGTAAGAGAT 
TCTGGACCAA CCTTCAACTA CCTTCTTGAT ATGCCTCTTT GGTATCTCAC CAAGGAGAAG 
AAGGATGAAC TGTGCAAGCT GAGAAATGAA AAAGAGCAAG AACTAGAAAC ATTAAAGAGA 
AAGAGTCCAA CAGATTTGTG GAAAGAAGAC TTGGCTACTT TTGTTGAAGA GCTAGAGGCT 
GTTGAAACAA AGGAAAAACA AGAAGAACAA ATAGGACTTC CAGGGAAAGG AGGGAAAGGA 
AAGGGGAAAA AAACACAAAT GGCTGAAGTT TTGCCTTCTC CTTGCGGAAA AAGAGTCATC 
CCCCGAGTGA CCATAGAGAT GAAAGCAGAG GCAGAAAAGA AAAATAAAAA GAAAATTAAG 
AATGAAAACA CTGAAGGGAG CCCTCAAGAA GAGAGTATCG AAATAGAAGG TCTGAAACAA 
AGGTTGGAGA AGAAACAGAA AAGAGAACCA GGTACCAAGA CAAAGAAGCA AACTACATTG 
CCGTTTAAAC CCATCAAAAA AGGAAAGAAG AGAAACCCCT GGTCTGATTC AGAATCAGAT 
ATGAGCAGTA ATGAAAGTAA CTTTGATGTC CCTCCACGAG AGGTAGAGCC ACGGAGAGCA 
GCAACAAAAA CCAAATTCAC AGTGGATTTG GATTTAGACG ACAGTTTCTC AGATTTTGAA 
GAAAGAATTC AAGATGAGGA TTCTGTTCCA TCAGATGTCA GCCCACATAA GACCAAAGCT 
TCCCCAAAAC GTACTGCCAA AGAACTGAAG CCACAGAAAA GTGCCCCCCC AGTAATGGAC 
CTTGATGCTG ATGATGCCAA GGACAGTGTA CCACTTTCTC CAGGTTCTAC TGCTGATTTC 
TTGGCTGAAA CTGAAATTGT AAACCCCCTT CCTAAAAAGA AGGTGGACGT GAAGAAGATA 
GCACCAAAAA GTCAGTCTTC CACCTCCACT ACAGGTGCAA AAAAAAGGGC TGCACCAAAA 
GGAACCAAAA AGGATCCAGG CTTAAATTCT GATGTCTCTC AAAAGCCTGA TCCCCCAAAA 
ACCAAAAATC ACCGCAAAAG GAAGCCATCC ACTTCTGATG ATTCCGACTC TAATTTTGAG 
AAGATGATTT CTAAAGCAGC CACAAACAAG AAATCCAAGG GGGAGAGTGA AGACTTCCAT 
CTGGACCTAG ACTCAGGTGT GGCTCCTCGG GCCAAATCTG GACGTGCAAG GAAACCTATA 
AAGTACCTTG AAGAATCAGA TGAAGATGAT CTGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP2A ( XM_537646.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_537646.2 Canis lupus familiaris DNA topoisomerase II alpha (TOP2A), mRNA.

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CloneID	OCe11751
Clone ID Related Accession (Same CDS sequence)	XM_537646.4 , XM_537646.5
Accession Version	XM_537646.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	DNA topoisomerase 2-alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_537646.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGT CACCACTGCA GCCGGTAAAT GAAAACATGC AAATCAGCAA AACAAAGAAA 
AATGAAGATG CTAAGAAAAG ACTGTCTATT GAAAGGATCT ATCAAAAGAA AACCCAACTG 
GAACATATTT TGCTCCGCCC GGACACCTAC ATTGGCTCTG TGGAATTAGT GACCCAGCAA 
ATGTGGGTTT ACGATGAAGA TATTGGCATG AACTATAGGG AAGTCACCTT TGTCCCTGGG 
TTGTACAAAA TCTTTGATGA GATTCTGGTC AATGCTGCAG ACAACAAACA AAGGGACCCA 
AAAATGTCTT GTATTAGAGT CACAATTGAC CCGGAAAACA ATTTAATTAG TATATGGAAT 
AATGGGAAAG GTATTCCTGT TGTTGAACAC AAAGTTGAGA AGATGTATGT CCCAGCACTC 
ATCTTTGGAC AGCTCCTAAC TTCTAGTAAC TATGATGATG ATGAAAAGAA AGTAACAGGT 
GGTCGAAATG GCTATGGGGC CAAATTGTGT AACATATTCA GTACCAAGTT TACTGTGGAA 
ACAGCCAGTA GAGAATACAA GAAAATGTTC AAACAGACGT GGATGGATAA CATGGGAAGG 
GCTGGTGAGA TGGAACTCAG GCCATTTAAT GGGGAAGATT ATACATGTAT TACCTTCCAG 
CCTGACTTGT CCAAGTTTAA AATGCAAAGC CTAGATAAAG ACATTGTTGC ACTGATGGTT 
AGAAGAGCAT ACGATATTGC TGGATCCACT AAAGATGTCA AAGTCTTTCT CAATGGAAAT 
AAACTACCAG TAAAAGGATT CCGTAGCTAC GTAGATATGT ATCTGAAGGA TAAGGTAGAT 
GAAACTGGCA ATCCATTGAA AATTATCCAT GAACAAGTAA ACCCCAGGTG GGAAGTATGT 
TTAACAATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTCG TCAACAGCAT TGCTACCTCC 
AAGGGTGGCA GACATGTTGA TTATATAGCT GATCAGATTG TGACTAAACT TGTTGATGTT 
GTGAAGAAGA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTGAA AAATCACATG 
TGGATTTTTG TGAATGCCTT GATTGAAAAC CCAACCTTTG ACTCTCAGAC GAAAGAAAAC 
ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAAT TAAGTGAAAA ATTCATCAAA 
GCTGCAACTG GCTGTGGTAT TGTGGAAAGC ATTCTAAACT GGGTAAAGTT TAAGGCCCAA 
ATCCAATTAA ACAAGAAGTG TTCAGCTGTA AAGCATAACA GAATCAAGGG AATTCCCAAA 
CTTGATGATG CCAATGACGC AGGGGGTCGA AACTCCAATG AGTGTACACT TATCCTGACT 
GAAGGAGACT CAGCTAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAA 
TATGGAGTTT TCCCACTTAG AGGAAAAATA CTCAATGTTC GAGAAGCTTC TCATAAACAG 
ATCATGGAAA ATGCTGAAAT TAATAATATC ATCAAGATCG TGGGTCTTCA GTACAAGAAA 
AACTATGACG ATGAAGATTC ATTGAAGACT CTTCGTTATG GGAAGATAAT GATTATGACA 
GATCAGGACC AAGATGGTTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCATAAC 
TGGCCCTCTC TTCTACGGCA TCGTTTTCTG GAGGAATTTA TCACTCCGAT TGTAAAGGTG 
TCTAAAAACA AACAAGAAAT GGCATTCTAT AGCCTTCCTG AATTTGAAGA ATGGAAGAGT 
TCTACTCCAA ATCATAAAAA ATGGAAAGTC AAGTACTACA AAGGTTTGGG TACCAGCACA 
TCAAAAGAAG CTAAAGAATA CTTTGCAAAT ATGAAAAGAC ATCGTATTCA GTTCAAATAC 
TCCGGTCCTG AAGATGATGC TGCCATCAGC TTGGCCTTTA GCAAGAAACA GATAGATGAT 
CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGAC AACGGAAGTT ACTTGGCCTT 
CCTGAGGATT ACTTGTATGG GCAAACTACC ACATATCTGA CATACAATGA CTTTATAAAT 
AAGGAACTTA TCCTGTTCTC AAATTCTGAT AACGAGAGAT CTATCCCATC TATGGTGGAT 
GGCTTGAAAC CAGGTCAAAG AAAGGTTTTG TTTACCTGTT TCAAACGGAA TGACAAGCGA 
GAGGTGAAGG TTGCCCAGTT GGCTGGGTCA GTGGCTGAGA TGTCCTCTTA TCATCATGGT 
GAGATGTCAC TAATGATGAC CATTATAAAT TTGGCTCAGA ATTTTGTGGG TAGCAATAAT 
CTGAACCTCT TGCAGCCCAT TGGTCAGTTT GGTACTAGGC TGCACGGTGG CAAGGATTCT 
GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTATT GTTTCCACCA 
AAAGATGATC AGACTTTAAA GTTTTTATAT GATGACAATC AACGTGTTGA ACCTGAATGG 
TACATTCCCA TTATACCCAT GGTGCTGATA AATGGTGCTG AAGGAATTGG TACTGGGTGG 
TCCTGCAAAA TCCCCAACTT TGATATTCGT GAAGTTGTAA ATAATATCAG GCGTTTGATG 
GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAGGGG TACTATTGAA 
GAGCTGGCTC CAAATCAGTA TGTGATTAGT GGTGAAGTGG CTATACTTAA TTCCACAACC 
ATTGAAATCT CAGAGCTTCC TGTCAGAACA TGGACCCAGA CCTATAAAGA ACAGGTTCTA 
GAACCCATGT TGAATGGCAC TGAGAAGACA CCACCTCTCA TAACAGATTA TAGGGAGTAC 
CATACAGACA CCACTGTGAA GTTTGTTGTG AAGATGACTG AAGAGAAACT GGCAGAGGCA 
GAGAGAGTGG GGCTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCCATG 
GTGCTTTTTG ACCATGTAGG TTGTTTAAAG AAATATGACA CAGTGTTGGA TATTCTAAGA 
GACTTCTTTG AACTAAGGCT TAAATATTAT GGATTAAGAA AAGAATGGCT TCTAGGAATG 
CTTGGCGCTG AATCTGCTAA ACTGAACAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT 
GGCAAAATAA TTATTGAAAA TAAGCCTAAG AAAGAATTAA TTAAAGTTCT GATTCAGAGG 
GGATATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCTC AGCAAAAGGT ACCAGATGAA 
GAAGAGAATG AAGAGAGTGA CAATGAAAAT GAAGCTGAAA AGAGTGACTC TGTAAGAGAT 
TCTGGACCAA CCTTCAACTA CCTTCTTGAT ATGCCTCTTT GGTATCTCAC CAAGGAGAAG 
AAGGATGAAC TGTGCAAGCT GAGAAATGAA AAAGAGCAAG AACTAGAAAC ATTAAAGAGA 
AAGAGTCCAA CAGATTTGTG GAAAGAAGAC TTGGCTACTT TTGTTGAAGA GCTAGAGGCT 
GTTGAAACAA AGGAAAAACA AGAAGAACAA ATAGGACTTC CAGGGAAAGG AGGGAAAGGA 
AAGGGGAAAA AAACACAAAT GGCTGAAGTT TTGCCTTCTC CTTGCGGAAA AAGAGTCATC 
CCCCGAGTGA CCATAGAGAT GAAAGCAGAG GCAGAAAAGA AAAATAAAAA GAAAATTAAG 
AATGAAAACA CTGAAGGGAG CCCTCAAGAA GAGAGTATCG AAATAGAAGG TCTGAAACAA 
AGGTTGGAGA AGAAACAGAA AAGAGAACCA GGTACCAAGA CAAAGAAGCA AACTACATTG 
CCGTTTAAAC CCATCAAAAA AGGAAAGAAG AGAAACCCCT GGTCTGATTC AGAATCAGAT 
ATGAGCAGTA ATGAAAGTAA CTTTGATGTC CCTCCACGAG AGGTAGAGCC ACGGAGAGCA 
GCAACAAAAA CCAAATTCAC AGTGGATTTG GATTTAGACG ACAGTTTCTC AGATTTTGAA 
GAAAGAATTC AAGATGAGGA TTCTGTTCCA TCAGATGTCA GCCCACATAA GACCAAAGCT 
TCCCCAAAAC GTACTGCCAA AGAACTGAAG CCACAGAAAA GTGCCCCCCC AGTAATGGAC 
CTTGATGCTG ATGATGCCAA GGACAGTGTA CCACTTTCTC CAGGTTCTAC TGCTGATTTC 
TTGGCTGAAA CTGAAATTGT AAACCCCCTT CCTAAAAAGA AGGTGGACGT GAAGAAGATA 
GCACCAAAAA GTCAGTCTTC CACCTCCACT ACAGGTGCAA AAAAAAGGGC TGCACCAAAA 
GGAACCAAAA AGGATCCAGG CTTAAATTCT GATGTCTCTC AAAAGCCTGA TCCCCCAAAA 
ACCAAAAATC ACCGCAAAAG GAAGCCATCC ACTTCTGATG ATTCCGACTC TAATTTTGAG 
AAGATGATTT CTAAAGCAGC CACAAACAAG AAATCCAAGG GGGAGAGTGA AGACTTCCAT 
CTGGACCTAG ACTCAGGTGT GGCTCCTCGG GCCAAATCTG GACGTGCAAG GAAACCTATA 
AAGTACCTTG AAGAATCAGA TGAAGATGAT CTGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_537646.2
CDS	138..4736
Translation	MEVSPLQPVNENMQISKTKKNEDAKKRLSIERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDIGMNYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGEMELRPFNGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNKLPVKGFRSYVDMYLKDKVDETGNPLKIIHEQVNPRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYIADQIVTKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQVKSFGSTCQLSEKFIKAATGCGIVESILNWVKFKAQIQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSNECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYDDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEMAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFANMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTHFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPKDDQTLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDIREVVNNIRRLMDGEEPLPMLPSYKNFRGTIEELAPNQYVISGEVAILNSTTIEISELPVRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMTEEKLAEAERVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNENEAEKSDSVRDSGPTFNYLLDMPLWYLTKEKKDELCKLRNEKEQELETLKRKSPTDLWKEDLATFVEELEAVETKEKQEEQIGLPGKGGKGKGKKTQMAEVLPSPCGKRVIPRVTIEMKAEAEKKNKKKIKNENTEGSPQEESIEIEGLKQRLEKKQKREPGTKTKKQTTLPFKPIKKGKKRNPWSDSESDMSSNESNFDVPPREVEPRRAATKTKFTVDLDLDDSFSDFEERIQDEDSVPSDVSPHKTKASPKRTAKELKPQKSAPPVMDLDADDAKDSVPLSPGSTADFLAETEIVNPLPKKKVDVKKIAPKSQSSTSTTGAKKRAAPKGTKKDPGLNSDVSQKPDPPKTKNHRKRKPSTSDDSDSNFEKMISKAATNKKSKGESEDFHLDLDSGVAPRAKSGRARKPIKYLEESDEDDLF

Target ORF information:

RefSeq Version	XM_537646.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris DNA topoisomerase II alpha (TOP2A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_537646.5

ORF Insert Sequence:

ATGGAGGTGT CACCACTGCA GCCGGTAAAT GAAAACATGC AAATCAGCAA AACAAAGAAA 
AATGAAGATG CTAAGAAAAG ACTGTCTATT GAAAGGATCT ATCAAAAGAA AACCCAACTG 
GAACATATTT TGCTCCGCCC GGACACCTAC ATTGGCTCTG TGGAATTAGT GACCCAGCAA 
ATGTGGGTTT ACGATGAAGA TATTGGCATG AACTATAGGG AAGTCACCTT TGTCCCTGGG 
TTGTACAAAA TCTTTGATGA GATTCTGGTC AATGCTGCAG ACAACAAACA AAGGGACCCA 
AAAATGTCTT GTATTAGAGT CACAATTGAC CCGGAAAACA ATTTAATTAG TATATGGAAT 
AATGGGAAAG GTATTCCTGT TGTTGAACAC AAAGTTGAGA AGATGTATGT CCCAGCACTC 
ATCTTTGGAC AGCTCCTAAC TTCTAGTAAC TATGATGATG ATGAAAAGAA AGTAACAGGT 
GGTCGAAATG GCTATGGGGC CAAATTGTGT AACATATTCA GTACCAAGTT TACTGTGGAA 
ACAGCCAGTA GAGAATACAA GAAAATGTTC AAACAGACGT GGATGGATAA CATGGGAAGG 
GCTGGTGAGA TGGAACTCAG GCCATTTAAT GGGGAAGATT ATACATGTAT TACCTTCCAG 
CCTGACTTGT CCAAGTTTAA AATGCAAAGC CTAGATAAAG ACATTGTTGC ACTGATGGTT 
AGAAGAGCAT ACGATATTGC TGGATCCACT AAAGATGTCA AAGTCTTTCT CAATGGAAAT 
AAACTACCAG TAAAAGGATT CCGTAGCTAC GTAGATATGT ATCTGAAGGA TAAGGTAGAT 
GAAACTGGCA ATCCATTGAA AATTATCCAT GAACAAGTAA ACCCCAGGTG GGAAGTATGT 
TTAACAATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTCG TCAACAGCAT TGCTACCTCC 
AAGGGTGGCA GACATGTTGA TTATATAGCT GATCAGATTG TGACTAAACT TGTTGATGTT 
GTGAAGAAGA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTGAA AAATCACATG 
TGGATTTTTG TGAATGCCTT GATTGAAAAC CCAACCTTTG ACTCTCAGAC GAAAGAAAAC 
ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAAT TAAGTGAAAA ATTCATCAAA 
GCTGCAACTG GCTGTGGTAT TGTGGAAAGC ATTCTAAACT GGGTAAAGTT TAAGGCCCAA 
ATCCAATTAA ACAAGAAGTG TTCAGCTGTA AAGCATAACA GAATCAAGGG AATTCCCAAA 
CTTGATGATG CCAATGACGC AGGGGGTCGA AACTCCAATG AGTGTACACT TATCCTGACT 
GAAGGAGACT CAGCTAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAA 
TATGGAGTTT TCCCACTTAG AGGAAAAATA CTCAATGTTC GAGAAGCTTC TCATAAACAG 
ATCATGGAAA ATGCTGAAAT TAATAATATC ATCAAGATCG TGGGTCTTCA GTACAAGAAA 
AACTATGACG ATGAAGATTC ATTGAAGACT CTTCGTTATG GGAAGATAAT GATTATGACA 
GATCAGGACC AAGATGGTTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCATAAC 
TGGCCCTCTC TTCTACGGCA TCGTTTTCTG GAGGAATTTA TCACTCCGAT TGTAAAGGTG 
TCTAAAAACA AACAAGAAAT GGCATTCTAT AGCCTTCCTG AATTTGAAGA ATGGAAGAGT 
TCTACTCCAA ATCATAAAAA ATGGAAAGTC AAGTACTACA AAGGTTTGGG TACCAGCACA 
TCAAAAGAAG CTAAAGAATA CTTTGCAAAT ATGAAAAGAC ATCGTATTCA GTTCAAATAC 
TCCGGTCCTG AAGATGATGC TGCCATCAGC TTGGCCTTTA GCAAGAAACA GATAGATGAT 
CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGAC AACGGAAGTT ACTTGGCCTT 
CCTGAGGATT ACTTGTATGG GCAAACTACC ACATATCTGA CATACAATGA CTTTATAAAT 
AAGGAACTTA TCCTGTTCTC AAATTCTGAT AACGAGAGAT CTATCCCATC TATGGTGGAT 
GGCTTGAAAC CAGGTCAAAG AAAGGTTTTG TTTACCTGTT TCAAACGGAA TGACAAGCGA 
GAGGTGAAGG TTGCCCAGTT GGCTGGGTCA GTGGCTGAGA TGTCCTCTTA TCATCATGGT 
GAGATGTCAC TAATGATGAC CATTATAAAT TTGGCTCAGA ATTTTGTGGG TAGCAATAAT 
CTGAACCTCT TGCAGCCCAT TGGTCAGTTT GGTACTAGGC TGCACGGTGG CAAGGATTCT 
GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTATT GTTTCCACCA 
AAAGATGATC AGACTTTAAA GTTTTTATAT GATGACAATC AACGTGTTGA ACCTGAATGG 
TACATTCCCA TTATACCCAT GGTGCTGATA AATGGTGCTG AAGGAATTGG TACTGGGTGG 
TCCTGCAAAA TCCCCAACTT TGATATTCGT GAAGTTGTAA ATAATATCAG GCGTTTGATG 
GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAGGGG TACTATTGAA 
GAGCTGGCTC CAAATCAGTA TGTGATTAGT GGTGAAGTGG CTATACTTAA TTCCACAACC 
ATTGAAATCT CAGAGCTTCC TGTCAGAACA TGGACCCAGA CCTATAAAGA ACAGGTTCTA 
GAACCCATGT TGAATGGCAC TGAGAAGACA CCACCTCTCA TAACAGATTA TAGGGAGTAC 
CATACAGACA CCACTGTGAA GTTTGTTGTG AAGATGACTG AAGAGAAACT GGCAGAGGCA 
GAGAGAGTGG GGCTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCCATG 
GTGCTTTTTG ACCATGTAGG TTGTTTAAAG AAATATGACA CAGTGTTGGA TATTCTAAGA 
GACTTCTTTG AACTAAGGCT TAAATATTAT GGATTAAGAA AAGAATGGCT TCTAGGAATG 
CTTGGCGCTG AATCTGCTAA ACTGAACAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT 
GGCAAAATAA TTATTGAAAA TAAGCCTAAG AAAGAATTAA TTAAAGTTCT GATTCAGAGG 
GGATATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCTC AGCAAAAGGT ACCAGATGAA 
GAAGAGAATG AAGAGAGTGA CAATGAAAAT GAAGCTGAAA AGAGTGACTC TGTAAGAGAT 
TCTGGACCAA CCTTCAACTA CCTTCTTGAT ATGCCTCTTT GGTATCTCAC CAAGGAGAAG 
AAGGATGAAC TGTGCAAGCT GAGAAATGAA AAAGAGCAAG AACTAGAAAC ATTAAAGAGA 
AAGAGTCCAA CAGATTTGTG GAAAGAAGAC TTGGCTACTT TTGTTGAAGA GCTAGAGGCT 
GTTGAAACAA AGGAAAAACA AGAAGAACAA ATAGGACTTC CAGGGAAAGG AGGGAAAGGA 
AAGGGGAAAA AAACACAAAT GGCTGAAGTT TTGCCTTCTC CTTGCGGAAA AAGAGTCATC 
CCCCGAGTGA CCATAGAGAT GAAAGCAGAG GCAGAAAAGA AAAATAAAAA GAAAATTAAG 
AATGAAAACA CTGAAGGGAG CCCTCAAGAA GAGAGTATCG AAATAGAAGG TCTGAAACAA 
AGGTTGGAGA AGAAACAGAA AAGAGAACCA GGTACCAAGA CAAAGAAGCA AACTACATTG 
CCGTTTAAAC CCATCAAAAA AGGAAAGAAG AGAAACCCCT GGTCTGATTC AGAATCAGAT 
ATGAGCAGTA ATGAAAGTAA CTTTGATGTC CCTCCACGAG AGGTAGAGCC ACGGAGAGCA 
GCAACAAAAA CCAAATTCAC AGTGGATTTG GATTTAGACG ACAGTTTCTC AGATTTTGAA 
GAAAGAATTC AAGATGAGGA TTCTGTTCCA TCAGATGTCA GCCCACATAA GACCAAAGCT 
TCCCCAAAAC GTACTGCCAA AGAACTGAAG CCACAGAAAA GTGCCCCCCC AGTAATGGAC 
CTTGATGCTG ATGATGCCAA GGACAGTGTA CCACTTTCTC CAGGTTCTAC TGCTGATTTC 
TTGGCTGAAA CTGAAATTGT AAACCCCCTT CCTAAAAAGA AGGTGGACGT GAAGAAGATA 
GCACCAAAAA GTCAGTCTTC CACCTCCACT ACAGGTGCAA AAAAAAGGGC TGCACCAAAA 
GGAACCAAAA AGGATCCAGG CTTAAATTCT GATGTCTCTC AAAAGCCTGA TCCCCCAAAA 
ACCAAAAATC ACCGCAAAAG GAAGCCATCC ACTTCTGATG ATTCCGACTC TAATTTTGAG 
AAGATGATTT CTAAAGCAGC CACAAACAAG AAATCCAAGG GGGAGAGTGA AGACTTCCAT 
CTGGACCTAG ACTCAGGTGT GGCTCCTCGG GCCAAATCTG GACGTGCAAG GAAACCTATA 
AAGTACCTTG AAGAATCAGA TGAAGATGAT CTGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP2A ( XM_005624533.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005624590.1 Canis lupus familiaris topoisomerase (DNA) II alpha (TOP2A), transcript variant X3, mRNA.

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CloneID	OCe11752
Clone ID Related Accession (Same CDS sequence)	XM_005624533.2
Accession Version	XM_005624533.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	DNA topoisomerase 2-alpha isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624533.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAATCA GCAAAACAAA GAAAAATGAA GATGCTAAGA AAAGACTGTC TATTGAAAGG 
ATCTATCAAA AGAAAACCCA ACTGGAACAT ATTTTGCTCC GCCCGGACAC CTACATTGGC 
TCTGTGGAAT TAGTGACCCA GCAAATGTGG GTTTACGATG AAGATATTGG CATGAACTAT 
AGGGAAGTCA CCTTTGTCCC TGGGTTGTAC AAAATCTTTG ATGAGATTCT GGTCAATGCT 
GCAGACAACA AACAAAGGGA CCCAAAAATG TCTTGTATTA GAGTCACAAT TGACCCGGAA 
AACAATTTAA TTAGTATATG GAATAATGGG AAAGGTATTC CTGTTGTTGA ACACAAAGTT 
GAGAAGATGT ATGTCCCAGC ACTCATCTTT GGACAGCTCC TAACTTCTAG TAACTATGAT 
GATGATGAAA AGAAAGTAAC AGGTGGTCGA AATGGCTATG GGGCCAAATT GTGTAACATA 
TTCAGTACCA AGTTTACTGT GGAAACAGCC AGTAGAGAAT ACAAGAAAAT GTTCAAACAG 
ACGTGGATGG ATAACATGGG AAGGGCTGGT GAGATGGAAC TCAGGCCATT TAATGGGGAA 
GATTATACAT GTATTACCTT CCAGCCTGAC TTGTCCAAGT TTAAAATGCA AAGCCTAGAT 
AAAGACATTG TTGCACTGAT GGTTAGAAGA GCATACGATA TTGCTGGATC CACTAAAGAT 
GTCAAAGTCT TTCTCAATGG AAATAAACTA CCAGTAAAAG GATTCCGTAG CTACGTAGAT 
ATGTATCTGA AGGATAAGGT AGATGAAACT GGCAATCCAT TGAAAATTAT CCATGAACAA 
GTAAACCCCA GGTGGGAAGT ATGTTTAACA ATGAGTGAAA AAGGCTTTCA GCAAATTAGC 
TTCGTCAACA GCATTGCTAC CTCCAAGGGT GGCAGACATG TTGATTATAT AGCTGATCAG 
ATTGTGACTA AACTTGTTGA TGTTGTGAAG AAGAAGAACA AGGGTGGTGT TGCAGTAAAA 
GCACATCAGG TGAAAAATCA CATGTGGATT TTTGTGAATG CCTTGATTGA AAACCCAACC 
TTTGACTCTC AGACGAAAGA AAACATGACT TTACAAGTCA AGAGCTTTGG ATCAACATGC 
CAATTAAGTG AAAAATTCAT CAAAGCTGCA ACTGGCTGTG GTATTGTGGA AAGCATTCTA 
AACTGGGTAA AGTTTAAGGC CCAAATCCAA TTAAACAAGA AGTGTTCAGC TGTAAAGCAT 
AACAGAATCA AGGGAATTCC CAAACTTGAT GATGCCAATG ACGCAGGGGG TCGAAACTCC 
AATGAGTGTA CACTTATCCT GACTGAAGGA GACTCAGCTA AAACTTTGGC TGTTTCAGGC 
CTTGGTGTGG TTGGGAGAGA CAAATATGGA GTTTTCCCAC TTAGAGGAAA AATACTCAAT 
GTTCGAGAAG CTTCTCATAA ACAGATCATG GAAAATGCTG AAATTAATAA TATCATCAAG 
ATCGTGGGTC TTCAGTACAA GAAAAACTAT GACGATGAAG ATTCATTGAA GACTCTTCGT 
TATGGGAAGA TAATGATTAT GACAGATCAG GACCAAGATG GTTCCCATAT CAAAGGCTTG 
CTGATTAATT TTATCCATCA TAACTGGCCC TCTCTTCTAC GGCATCGTTT TCTGGAGGAA 
TTTATCACTC CGATTGTAAA GGTGTCTAAA AACAAACAAG AAATGGCATT CTATAGCCTT 
CCTGAATTTG AAGAATGGAA GAGTTCTACT CCAAATCATA AAAAATGGAA AGTCAAGTAC 
TACAAAGGTT TGGGTACCAG CACATCAAAA GAAGCTAAAG AATACTTTGC AAATATGAAA 
AGACATCGTA TTCAGTTCAA ATACTCCGGT CCTGAAGATG ATGCTGCCAT CAGCTTGGCC 
TTTAGCAAGA AACAGATAGA TGATCGAAAG GAATGGTTAA CTCATTTCAT GGAAGATAGA 
AGACAACGGA AGTTACTTGG CCTTCCTGAG GATTACTTGT ATGGGCAAAC TACCACATAT 
CTGACATACA ATGACTTTAT AAATAAGGAA CTTATCCTGT TCTCAAATTC TGATAACGAG 
AGATCTATCC CATCTATGGT GGATGGCTTG AAACCAGGTC AAAGAAAGGT TTTGTTTACC 
TGTTTCAAAC GGAATGACAA GCGAGAGGTG AAGGTTGCCC AGTTGGCTGG GTCAGTGGCT 
GAGATGTCCT CTTATCATCA TGGTGAGATG TCACTAATGA TGACCATTAT AAATTTGGCT 
CAGAATTTTG TGGGTAGCAA TAATCTGAAC CTCTTGCAGC CCATTGGTCA GTTTGGTACT 
AGGCTGCACG GTGGCAAGGA TTCTGCTAGT CCTCGATACA TCTTCACAAT GCTCAGCCCT 
TTGGCTCGGT TATTGTTTCC ACCAAAAGAT GATCAGACTT TAAAGTTTTT ATATGATGAC 
AATCAACGTG TTGAACCTGA ATGGTACATT CCCATTATAC CCATGGTGCT GATAAATGGT 
GCTGAAGGAA TTGGTACTGG GTGGTCCTGC AAAATCCCCA ACTTTGATAT TCGTGAAGTT 
GTAAATAATA TCAGGCGTTT GATGGATGGA GAAGAACCTT TGCCAATGCT TCCAAGTTAC 
AAGAACTTCA GGGGTACTAT TGAAGAGCTG GCTCCAAATC AGTATGTGAT TAGTGGTGAA 
GTGGCTATAC TTAATTCCAC AACCATTGAA ATCTCAGAGC TTCCTGTCAG AACATGGACC 
CAGACCTATA AAGAACAGGT TCTAGAACCC ATGTTGAATG GCACTGAGAA GACACCACCT 
CTCATAACAG ATTATAGGGA GTACCATACA GACACCACTG TGAAGTTTGT TGTGAAGATG 
ACTGAAGAGA AACTGGCAGA GGCAGAGAGA GTGGGGCTAC ACAAAGTCTT CAAACTCCAA 
ACTAGTCTCA CATGCAACTC CATGGTGCTT TTTGACCATG TAGGTTGTTT AAAGAAATAT 
GACACAGTGT TGGATATTCT AAGAGACTTC TTTGAACTAA GGCTTAAATA TTATGGATTA 
AGAAAAGAAT GGCTTCTAGG AATGCTTGGC GCTGAATCTG CTAAACTGAA CAATCAGGCT 
CGCTTTATCT TAGAGAAAAT AGATGGCAAA ATAATTATTG AAAATAAGCC TAAGAAAGAA 
TTAATTAAAG TTCTGATTCA GAGGGGATAT GATTCGGATC CTGTGAAGGC TTGGAAAGAA 
GCTCAGCAAA AGGTACCAGA TGAAGAAGAG AATGAAGAGA GTGACAATGA AAATGAAGCT 
GAAAAGAGTG ACTCTGTAAG AGATTCTGGA CCAACCTTCA ACTACCTTCT TGATATGCCT 
CTTTGGTATC TCACCAAGGA GAAGAAGGAT GAACTGTGCA AGCTGAGAAA TGAAAAAGAG 
CAAGAACTAG AAACATTAAA GAGAAAGAGT CCAACAGATT TGTGGAAAGA AGACTTGGCT 
ACTTTTGTTG AAGAGCTAGA GGCTGTTGAA ACAAAGGAAA AACAAGAAGA ACAAATAGGA 
CTTCCAGGGA AAGGAGGGAA AGGAAAGGGG AAAAAAACAC AAATGGCTGA AGTTTTGCCT 
TCTCCTTGCG GAAAAAGAGT CATCCCCCGA GTGACCATAG AGATGAAAGC AGAGGCAGAA 
AAGAAAAATA AAAAGAAAAT TAAGAATGAA AACACTGAAG GGAGCCCTCA AGAAGAGAGT 
ATCGAAATAG AAGGTCTGAA ACAAAGGTTG GAGAAGAAAC AGAAAAGAGA ACCAGGTACC 
AAGACAAAGA AGCAAACTAC ATTGCCGTTT AAACCCATCA AAAAAGGAAA GAAGAGAAAC 
CCCTGGTCTG ATTCAGAATC AGATATGAGC AGTAATGAAA GTAACTTTGA TGTCCCTCCA 
CGAGAGGTAG AGCCACGGAG AGCAGCAACA AAAACCAAAT TCACAGTGGA TTTGGATTTA 
GACGACAGTT TCTCAGATTT TGAAGAAAGA ATTCAAGATG AGGATTCTGT TCCATCAGAT 
GTCAGCCCAC ATAAGACCAA AGCTTCCCCA AAACGTACTG CCAAAGAACT GAAGCCACAG 
AAAAGTGCCC CCCCAGTAAT GGACCTTGAT GCTGATGATG CCAAGGACAG TGTACCACTT 
TCTCCAGGTT CTACTGCTGA TTTCTTGGCT GAAACTGAAA TTGTAAACCC CCTTCCTAAA 
AAGAAGGTGG ACGTGAAGAA GATAGCACCA AAAAGTCAGT CTTCCACCTC CACTACAGGT 
GCAAAAAAAA GGGCTGCACC AAAAGGAACC AAAAAGGATC CAGGCTTAAA TTCTGATGTC 
TCTCAAAAGC CTGATCCCCC AAAAACCAAA AATCACCGCA AAAGGAAGCC ATCCACTTCT 
GATGATTCCG ACTCTAATTT TGAGAAGATG ATTTCTAAAG CAGCCACAAA CAAGAAATCC 
AAGGGGGAGA GTGAAGACTT CCATCTGGAC CTAGACTCAG GTGTGGCTCC TCGGGCCAAA 
TCTGGACGTG CAAGGAAACC TATAAAGTAC CTTGAAGAAT CAGATGAAGA TGATCTGTTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624590.1
CDS	151..4713
Translation	MQISKTKKNEDAKKRLSIERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDIGMNYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGEMELRPFNGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNKLPVKGFRSYVDMYLKDKVDETGNPLKIIHEQVNPRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYIADQIVTKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQVKSFGSTCQLSEKFIKAATGCGIVESILNWVKFKAQIQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSNECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYDDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEMAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFANMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTHFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPKDDQTLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDIREVVNNIRRLMDGEEPLPMLPSYKNFRGTIEELAPNQYVISGEVAILNSTTIEISELPVRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMTEEKLAEAERVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNENEAEKSDSVRDSGPTFNYLLDMPLWYLTKEKKDELCKLRNEKEQELETLKRKSPTDLWKEDLATFVEELEAVETKEKQEEQIGLPGKGGKGKGKKTQMAEVLPSPCGKRVIPRVTIEMKAEAEKKNKKKIKNENTEGSPQEESIEIEGLKQRLEKKQKREPGTKTKKQTTLPFKPIKKGKKRNPWSDSESDMSSNESNFDVPPREVEPRRAATKTKFTVDLDLDDSFSDFEERIQDEDSVPSDVSPHKTKASPKRTAKELKPQKSAPPVMDLDADDAKDSVPLSPGSTADFLAETEIVNPLPKKKVDVKKIAPKSQSSTSTTGAKKRAAPKGTKKDPGLNSDVSQKPDPPKTKNHRKRKPSTSDDSDSNFEKMISKAATNKKSKGESEDFHLDLDSGVAPRAKSGRARKPIKYLEESDEDDLF

Target ORF information:

RefSeq Version	XM_005624533.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris topoisomerase (DNA) II alpha (TOP2A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624533.2

ORF Insert Sequence:

ATGCAAATCA GCAAAACAAA GAAAAATGAA GATGCTAAGA AAAGACTGTC TATTGAAAGG 
ATCTATCAAA AGAAAACCCA ACTGGAACAT ATTTTGCTCC GCCCGGACAC CTACATTGGC 
TCTGTGGAAT TAGTGACCCA GCAAATGTGG GTTTACGATG AAGATATTGG CATGAACTAT 
AGGGAAGTCA CCTTTGTCCC TGGGTTGTAC AAAATCTTTG ATGAGATTCT GGTCAATGCT 
GCAGACAACA AACAAAGGGA CCCAAAAATG TCTTGTATTA GAGTCACAAT TGACCCGGAA 
AACAATTTAA TTAGTATATG GAATAATGGG AAAGGTATTC CTGTTGTTGA ACACAAAGTT 
GAGAAGATGT ATGTCCCAGC ACTCATCTTT GGACAGCTCC TAACTTCTAG TAACTATGAT 
GATGATGAAA AGAAAGTAAC AGGTGGTCGA AATGGCTATG GGGCCAAATT GTGTAACATA 
TTCAGTACCA AGTTTACTGT GGAAACAGCC AGTAGAGAAT ACAAGAAAAT GTTCAAACAG 
ACGTGGATGG ATAACATGGG AAGGGCTGGT GAGATGGAAC TCAGGCCATT TAATGGGGAA 
GATTATACAT GTATTACCTT CCAGCCTGAC TTGTCCAAGT TTAAAATGCA AAGCCTAGAT 
AAAGACATTG TTGCACTGAT GGTTAGAAGA GCATACGATA TTGCTGGATC CACTAAAGAT 
GTCAAAGTCT TTCTCAATGG AAATAAACTA CCAGTAAAAG GATTCCGTAG CTACGTAGAT 
ATGTATCTGA AGGATAAGGT AGATGAAACT GGCAATCCAT TGAAAATTAT CCATGAACAA 
GTAAACCCCA GGTGGGAAGT ATGTTTAACA ATGAGTGAAA AAGGCTTTCA GCAAATTAGC 
TTCGTCAACA GCATTGCTAC CTCCAAGGGT GGCAGACATG TTGATTATAT AGCTGATCAG 
ATTGTGACTA AACTTGTTGA TGTTGTGAAG AAGAAGAACA AGGGTGGTGT TGCAGTAAAA 
GCACATCAGG TGAAAAATCA CATGTGGATT TTTGTGAATG CCTTGATTGA AAACCCAACC 
TTTGACTCTC AGACGAAAGA AAACATGACT TTACAAGTCA AGAGCTTTGG ATCAACATGC 
CAATTAAGTG AAAAATTCAT CAAAGCTGCA ACTGGCTGTG GTATTGTGGA AAGCATTCTA 
AACTGGGTAA AGTTTAAGGC CCAAATCCAA TTAAACAAGA AGTGTTCAGC TGTAAAGCAT 
AACAGAATCA AGGGAATTCC CAAACTTGAT GATGCCAATG ACGCAGGGGG TCGAAACTCC 
AATGAGTGTA CACTTATCCT GACTGAAGGA GACTCAGCTA AAACTTTGGC TGTTTCAGGC 
CTTGGTGTGG TTGGGAGAGA CAAATATGGA GTTTTCCCAC TTAGAGGAAA AATACTCAAT 
GTTCGAGAAG CTTCTCATAA ACAGATCATG GAAAATGCTG AAATTAATAA TATCATCAAG 
ATCGTGGGTC TTCAGTACAA GAAAAACTAT GACGATGAAG ATTCATTGAA GACTCTTCGT 
TATGGGAAGA TAATGATTAT GACAGATCAG GACCAAGATG GTTCCCATAT CAAAGGCTTG 
CTGATTAATT TTATCCATCA TAACTGGCCC TCTCTTCTAC GGCATCGTTT TCTGGAGGAA 
TTTATCACTC CGATTGTAAA GGTGTCTAAA AACAAACAAG AAATGGCATT CTATAGCCTT 
CCTGAATTTG AAGAATGGAA GAGTTCTACT CCAAATCATA AAAAATGGAA AGTCAAGTAC 
TACAAAGGTT TGGGTACCAG CACATCAAAA GAAGCTAAAG AATACTTTGC AAATATGAAA 
AGACATCGTA TTCAGTTCAA ATACTCCGGT CCTGAAGATG ATGCTGCCAT CAGCTTGGCC 
TTTAGCAAGA AACAGATAGA TGATCGAAAG GAATGGTTAA CTCATTTCAT GGAAGATAGA 
AGACAACGGA AGTTACTTGG CCTTCCTGAG GATTACTTGT ATGGGCAAAC TACCACATAT 
CTGACATACA ATGACTTTAT AAATAAGGAA CTTATCCTGT TCTCAAATTC TGATAACGAG 
AGATCTATCC CATCTATGGT GGATGGCTTG AAACCAGGTC AAAGAAAGGT TTTGTTTACC 
TGTTTCAAAC GGAATGACAA GCGAGAGGTG AAGGTTGCCC AGTTGGCTGG GTCAGTGGCT 
GAGATGTCCT CTTATCATCA TGGTGAGATG TCACTAATGA TGACCATTAT AAATTTGGCT 
CAGAATTTTG TGGGTAGCAA TAATCTGAAC CTCTTGCAGC CCATTGGTCA GTTTGGTACT 
AGGCTGCACG GTGGCAAGGA TTCTGCTAGT CCTCGATACA TCTTCACAAT GCTCAGCCCT 
TTGGCTCGGT TATTGTTTCC ACCAAAAGAT GATCAGACTT TAAAGTTTTT ATATGATGAC 
AATCAACGTG TTGAACCTGA ATGGTACATT CCCATTATAC CCATGGTGCT GATAAATGGT 
GCTGAAGGAA TTGGTACTGG GTGGTCCTGC AAAATCCCCA ACTTTGATAT TCGTGAAGTT 
GTAAATAATA TCAGGCGTTT GATGGATGGA GAAGAACCTT TGCCAATGCT TCCAAGTTAC 
AAGAACTTCA GGGGTACTAT TGAAGAGCTG GCTCCAAATC AGTATGTGAT TAGTGGTGAA 
GTGGCTATAC TTAATTCCAC AACCATTGAA ATCTCAGAGC TTCCTGTCAG AACATGGACC 
CAGACCTATA AAGAACAGGT TCTAGAACCC ATGTTGAATG GCACTGAGAA GACACCACCT 
CTCATAACAG ATTATAGGGA GTACCATACA GACACCACTG TGAAGTTTGT TGTGAAGATG 
ACTGAAGAGA AACTGGCAGA GGCAGAGAGA GTGGGGCTAC ACAAAGTCTT CAAACTCCAA 
ACTAGTCTCA CATGCAACTC CATGGTGCTT TTTGACCATG TAGGTTGTTT AAAGAAATAT 
GACACAGTGT TGGATATTCT AAGAGACTTC TTTGAACTAA GGCTTAAATA TTATGGATTA 
AGAAAAGAAT GGCTTCTAGG AATGCTTGGC GCTGAATCTG CTAAACTGAA CAATCAGGCT 
CGCTTTATCT TAGAGAAAAT AGATGGCAAA ATAATTATTG AAAATAAGCC TAAGAAAGAA 
TTAATTAAAG TTCTGATTCA GAGGGGATAT GATTCGGATC CTGTGAAGGC TTGGAAAGAA 
GCTCAGCAAA AGGTACCAGA TGAAGAAGAG AATGAAGAGA GTGACAATGA AAATGAAGCT 
GAAAAGAGTG ACTCTGTAAG AGATTCTGGA CCAACCTTCA ACTACCTTCT TGATATGCCT 
CTTTGGTATC TCACCAAGGA GAAGAAGGAT GAACTGTGCA AGCTGAGAAA TGAAAAAGAG 
CAAGAACTAG AAACATTAAA GAGAAAGAGT CCAACAGATT TGTGGAAAGA AGACTTGGCT 
ACTTTTGTTG AAGAGCTAGA GGCTGTTGAA ACAAAGGAAA AACAAGAAGA ACAAATAGGA 
CTTCCAGGGA AAGGAGGGAA AGGAAAGGGG AAAAAAACAC AAATGGCTGA AGTTTTGCCT 
TCTCCTTGCG GAAAAAGAGT CATCCCCCGA GTGACCATAG AGATGAAAGC AGAGGCAGAA 
AAGAAAAATA AAAAGAAAAT TAAGAATGAA AACACTGAAG GGAGCCCTCA AGAAGAGAGT 
ATCGAAATAG AAGGTCTGAA ACAAAGGTTG GAGAAGAAAC AGAAAAGAGA ACCAGGTACC 
AAGACAAAGA AGCAAACTAC ATTGCCGTTT AAACCCATCA AAAAAGGAAA GAAGAGAAAC 
CCCTGGTCTG ATTCAGAATC AGATATGAGC AGTAATGAAA GTAACTTTGA TGTCCCTCCA 
CGAGAGGTAG AGCCACGGAG AGCAGCAACA AAAACCAAAT TCACAGTGGA TTTGGATTTA 
GACGACAGTT TCTCAGATTT TGAAGAAAGA ATTCAAGATG AGGATTCTGT TCCATCAGAT 
GTCAGCCCAC ATAAGACCAA AGCTTCCCCA AAACGTACTG CCAAAGAACT GAAGCCACAG 
AAAAGTGCCC CCCCAGTAAT GGACCTTGAT GCTGATGATG CCAAGGACAG TGTACCACTT 
TCTCCAGGTT CTACTGCTGA TTTCTTGGCT GAAACTGAAA TTGTAAACCC CCTTCCTAAA 
AAGAAGGTGG ACGTGAAGAA GATAGCACCA AAAAGTCAGT CTTCCACCTC CACTACAGGT 
GCAAAAAAAA GGGCTGCACC AAAAGGAACC AAAAAGGATC CAGGCTTAAA TTCTGATGTC 
TCTCAAAAGC CTGATCCCCC AAAAACCAAA AATCACCGCA AAAGGAAGCC ATCCACTTCT 
GATGATTCCG ACTCTAATTT TGAGAAGATG ATTTCTAAAG CAGCCACAAA CAAGAAATCC 
AAGGGGGAGA GTGAAGACTT CCATCTGGAC CTAGACTCAG GTGTGGCTCC TCGGGCCAAA 
TCTGGACGTG CAAGGAAACC TATAAAGTAC CTTGAAGAAT CAGATGAAGA TGATCTGTTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TOP2A cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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