enpp2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	enpp2
Entrez Gene ID	100332039
Full Name	ectonucleotide pyrophosphatase/phosphodiesterase 2
Synonyms	atx,zgc:63550
General protein information	Preferred Names ectonucleotide pyrophosphatase/phosphodiesterase 2 Names ectonucleotide pyrophosphatase/phosphodiesterase family member 2 autotaxin
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200603.1	NP_956897.1	ectonucleotide pyrophosphatase/phosphodiesterase family member 2 precursor
XM_009292082.2	XP_009290357.1	ectonucleotide pyrophosphatase/phosphodiesterase family member 2 isoform X1
XM_009292082.3	XP_009290357.1	ectonucleotide pyrophosphatase/phosphodiesterase family member 2 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ENPP2	XP_519922.2
Rattus norvegicus (Norway rat)	Enpp2	NP_476445.2
Danio rerio (zebrafish)	enpp2	XP_005173554.1
Homo sapiens (human)	ENPP2	NP_006200.3
Mus musculus (house mouse)	Enpp2	NP_001129549.1
Gallus gallus (chicken)	ENPP2	NP_001185591.1
Danio rerio (zebrafish)	enpp2	NP_956897.1
Xenopus tropicalis (tropical clawed frog)	enpp2	NP_001015936.1
	ENPP2	XP_002805517.1
Canis lupus familiaris (dog)	ENPP2	XP_539147.2
Bos taurus (cattle)	ENPP2	NP_001073762.1

Gene Ontology
Bibliography

Related articles in PubMed

An Adenosine Receptor for Olfaction in Fish.
Wakisaka N, Miyasaka N, Koide T, Masuda M, Hiraki-Kajiyama T, Yoshihara Y
Current biology : CB27(10)1437-1447.e4(2017 May)

Role of ectonucleotide pyrophosphatase/phosphodiesterase 2 in the midline axis formation of zebrafish.
Frisca F, Colquhoun D, Goldshmit Y, ?nk? ML, P?bay A, Kaslin J
Scientific reports637678(2016 Nov)

Autotaxin-Lysophosphatidic Acid Axis Acts Downstream of Apoprotein B Lipoproteins in Endothelial Cells.
Gibbs-Bar L, Tempelhof H, Ben-Hamo R, Ely Y, Brandis A, Hofi R, Almog G, Braun T, Feldmesser E, Efroni S, Yaniv K
Arteriosclerosis, thrombosis, and vascular biology36(10)2058-67(2016 Oct)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Autotaxin-Lysophosphatidic Acid axis acts downstream of Apoprotein B lipoproteins in endothelial cells.
Title: Autotaxin-Lysophosphatidic Acid Axis Acts Downstream of Apoprotein B Lipoproteins in Endothelial Cells.

When autotaxin (Atx), was overexpressed in zebrafish embryos by injecting atx mRNA, the embryos showed cardia bifida, a phenotype induced by down-regulation of sphingosine-1-phosphate signaling.
Title: Overexpression of autotaxin, a lysophosphatidic acid-producing enzyme, enhances cardia bifida induced by hypo-sphingosine-1-phosphate signaling in zebrafish embryo.

results suggest a major role for the Atx/Lpar3 signaling axis in regulating KV formation, ciliogenesis and L-R asymmetry via a Wnt-dependent pathway
Title: Autotaxin/Lpar3 signaling regulates Kupffer's vesicle formation and left-right asymmetry in zebrafish.

This study demonstrate that the zebrafish ortholog to mammalian atx displays evolutionarily conserved expression pattern characteristics.
Title: Autotaxin/ENPP2 regulates oligodendrocyte differentiation in vivo in the developing zebrafish hindbrain.

the ATX-LPA-LPAR axis is a critical regulator of embryonic vascular development that is conserved in vertebrates
Title: Autotaxin regulates vascular development via multiple lysophosphatidic acid (LPA) receptors in zebrafish.

The following enpp2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the enpp2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa01435	NM_200603.1 Latest version!	Danio rerio ectonucleotide pyrophosphatase/phosphodiesterase 2 (enpp2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa21160	XM_009292082.3 Latest version!	Danio rerio ectonucleotide pyrophosphatase/phosphodiesterase 2 (enpp2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa21160	XM_009292082.2	Danio rerio ectonucleotide pyrophosphatase/phosphodiesterase 2 (enpp2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

enpp2 ( NM_200603.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956897.1 Danio rerio ectonucleotide pyrophosphatase/phosphodiesterase 2 (enpp2), mRNA.

Price & Availability↑

CloneID	ODa01435
Clone ID Related Accession (Same CDS sequence)	NM_200603.1
Accession Version	NM_200603.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Danio rerio(zebrafish)
Product	ectonucleotide pyrophosphatase/phosphodiesterase family member 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC056781.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC056781.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505392, SAMEA4476765 [ECO:0000348] ##Evidence-Data-END##

ATGCTGCAAC TTAAATGGGT ATTCTCCGTT ATGTGGCTTT TCTCACGGTT AACAGTGTGT 
AAGACTTATG TGGTCAGACG GTCCGGAAAG GCTGCATCAC CAGACGAATC GCTCTCAAAA 
AGTTTCTCTG ACCAGCCATT TACCAGCCTG GCAGGTTCTT GCAAAAAACG GTGTTTTGAG 
CTAGTGGAAG CTGATCCACC AAATTGTCGC TGTGACAACC TGTGTAAAAC ATACAATATG 
TGTTGCTCAG ACTTTGATGA CCACTGTCTA AAAACTGCAG GGGGTTTTGA GTGTAGTAAA 
GAGCGTTGTG GTGAAAATCG TAATGAGCAG CATGCCTGCC ACTGCTCAGA GGACTGCATG 
GCCAAAGGAG ACTGCTGCAC CAACTACAGG TCTCTTTGCA AAGGCGATGT TCCTTGGTTG 
CAAGAAGAGT GTGAAGAGAT TAAGAACCAC GAGTGTCCTG CTGGGTTTGT TCGACCTCCT 
GTCATCATGT TGTCTGTTGA TGGCTTTCGT GCATCTTACA TGAAAAGAGG AGGCACGGTG 
ATCCCCAACA TCGAGAAACT CCGATCCTGT GGAACACATG CACCATACAT GAGGCCTATG 
TACCCTACAA AAACCTACCC AAATTTGTAT ACAATTACAA CGGGGCTTTA TCCTGAATCG 
CATGGGATTG TGGGTAACTC CATTCATGAT CCAAGCTTTG ATGCCAACTT TAACTTCCGT 
GGGAAAGAGA AGCTTAATCA TCGATGGTGG GGAGGCCAGC CTATCTGGAT TACTGCAATG 
AAACAGGGAG TAAAGGCTGG TTCATTTTTC TGGCCAGTTG CCATTCCAAT GGAAAGAAGA 
GTTCTGACCA TGTTGCAATG GCTTCATCTC CCTGATGCAG AGAGGCCATA TCTTTATGCA 
ATGCATTCTG AACAGCTGGA TTCATATGGC CACAAACTAG GACCCCACAG CACAGAGCTT 
AACAGTGCAC TAAGGGATGT TGATAAGGTG ATTGGACAGC TGATGAATGG CCTGAAACAG 
ATGAAACTAC ACCGTTGTAT CAATATCATT TTGGTTGGAG ACCATGGTAT GGAGGAAGCT 
CATTGTGACA AAACAGAGTT CCTAAGTTCT TATATGTCCA ACACTGAGGA CCTCATCCTA 
ATTCCTGGCT CTCTGGGAAG AATCCGAGCC CGAAATCCCA ACAACTCTAA ATTTGATGCA 
AAAGCAGTTG TGGCAAACCT AACATGTAAA AAGCCAGACC AGCACTTTAA GCCATACTTA 
AAGCAGCATC TTCCCAAACG TTTGCACTAT GCCAATAACG ACAGAATTGA GGAGATTCAT 
TTAATGGTTG AAAGGAAATG GCACATTGCA CGGAAAATCA TGAAAACAAA AAGGAATCAT 
GAGAAATGTG GGTTTGCTGG TGATCACGGA TATGACAATA AGATCAACAG CATGCAGACA 
ATTTTCCTTG GATATGGACC AGCTTTCAAA TTCAAAACCA AAATTCCACC ATTCGAGAAT 
ATTGAGCTTT ATAATATCAT GTGTGATCTC CTTGGCTTGA AGCCAGCCCC TAACAATGGT 
ACACATGGTA GTCTTAACCA GCTCCTGAGG ACCCCTGTAT ACATACCCAA CATGCCTGAA 
GAAGTGACCA AGCCAAACCC TGCAGGACCA GTCATAGCTA TTAATGATGA CCTGGGCTGC 
ACTTGTGAAG ACAAGAACAA AGTGGACGAG CTCAATCAAC GACTGAGACA AGTTATTGAT 
GACAACAAGA ATCTTCCATA TGGGCGTCCA GCTGTTATGT TTCGCACCAA ATACTGCATT 
CTTCATCATA CTGACTACAT TAGTGGATAC AGTGAAGCTT TACATATGCC TCTTTGGACA 
TCTTATACCG TTTCCAAGCA GGTAGACTTT ACCCCTCTGA CTGATGCTCT GAGCAACTGC 
ATTCGACCTG ATAGTAGAGT TCCTACAGCT TACAGCCAGT CCTGCTCAAA CTACCGCACA 
GAAAAACAGA TCTCCTACAG TTTCCTCTAT CCACCTCAGT TGTCCTCCAC TCAGGAAGCG 
AGATATGATG CTGTTCTCAT TACCAACACT GTACCAATGT ATGCAGCATT CAAACGAGTG 
TGGAGTTATT TTCAGAAATC ACTTGTGAAA CGTTACGCTA GTGAGAAAAA TGGTGTGAAT 
GTTGTCACCG GGCCCATTTT CGACTATGAC TACAATGGTC TTAGAGATTC TGCAGAGACA 
ATAAAACAGT TTGTCAGTGG CTCCGTACAA ATCCCAACTC ATTACTTTGT TGTGGTTACA 
AGCTGCCTCG ATTACACACA GACCGTTGAC TCCTGTGTTG GTCCTCTCAG TGTGTTCTCC 
TTCATCCTTC CACACCGATC AGATAATGAA GAGACCTGCA ATAGTTCAGA AGATGAGTCA 
AAATGGGTGG AGGAACTGAT GAAGACTCAT ACAGCCCGCC TGCGAGATGT GGAGTTACTC 
ACAGGACTGG ACTTTTATCG CCGCACCAGC AGGACCTATG AGGAGATTCT CGCTCTGAAA 
ACGTACTTGC ACACATACGA AAGTGAGATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956897.1
CDS	62..2614
Translation	MLQLKWVFSVMWLFSRLTVCKTYVVRRSGKAASPDESLSKSFSDQPFTSLAGSCKKRCFELVEADPPNCRCDNLCKTYNMCCSDFDDHCLKTAGGFECSKERCGENRNEQHACHCSEDCMAKGDCCTNYRSLCKGDVPWLQEECEEIKNHECPAGFVRPPVIMLSVDGFRASYMKRGGTVIPNIEKLRSCGTHAPYMRPMYPTKTYPNLYTITTGLYPESHGIVGNSIHDPSFDANFNFRGKEKLNHRWWGGQPIWITAMKQGVKAGSFFWPVAIPMERRVLTMLQWLHLPDAERPYLYAMHSEQLDSYGHKLGPHSTELNSALRDVDKVIGQLMNGLKQMKLHRCINIILVGDHGMEEAHCDKTEFLSSYMSNTEDLILIPGSLGRIRARNPNNSKFDAKAVVANLTCKKPDQHFKPYLKQHLPKRLHYANNDRIEEIHLMVERKWHIARKIMKTKRNHEKCGFAGDHGYDNKINSMQTIFLGYGPAFKFKTKIPPFENIELYNIMCDLLGLKPAPNNGTHGSLNQLLRTPVYIPNMPEEVTKPNPAGPVIAINDDLGCTCEDKNKVDELNQRLRQVIDDNKNLPYGRPAVMFRTKYCILHHTDYISGYSEALHMPLWTSYTVSKQVDFTPLTDALSNCIRPDSRVPTAYSQSCSNYRTEKQISYSFLYPPQLSSTQEARYDAVLITNTVPMYAAFKRVWSYFQKSLVKRYASEKNGVNVVTGPIFDYDYNGLRDSAETIKQFVSGSVQIPTHYFVVVTSCLDYTQTVDSCVGPLSVFSFILPHRSDNEETCNSSEDESKWVEELMKTHTARLRDVELLTGLDFYRRTSRTYEEILALKTYLHTYESEI

Target ORF information:

RefSeq Version	NM_200603.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ectonucleotide pyrophosphatase/phosphodiesterase 2 (enpp2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200603.1

ORF Insert Sequence:

ATGCTGCAAC TTAAATGGGT ATTCTCCGTT ATGTGGCTTT TCTCACGGTT AACAGTGTGT 
AAGACTTATG TGGTCAGACG GTCCGGAAAG GCTGCATCAC CAGACGAATC GCTCTCAAAA 
AGTTTCTCTG ACCAGCCATT TACCAGCCTG GCAGGTTCTT GCAAAAAACG GTGTTTTGAG 
CTAGTGGAAG CTGATCCACC AAATTGTCGC TGTGACAACC TGTGTAAAAC ATACAATATG 
TGTTGCTCAG ACTTTGATGA CCACTGTCTA AAAACTGCAG GGGGTTTTGA GTGTAGTAAA 
GAGCGTTGTG GTGAAAATCG TAATGAGCAG CATGCCTGCC ACTGCTCAGA GGACTGCATG 
GCCAAAGGAG ACTGCTGCAC CAACTACAGG TCTCTTTGCA AAGGCGATGT TCCTTGGTTG 
CAAGAAGAGT GTGAAGAGAT TAAGAACCAC GAGTGTCCTG CTGGGTTTGT TCGACCTCCT 
GTCATCATGT TGTCTGTTGA TGGCTTTCGT GCATCTTACA TGAAAAGAGG AGGCACGGTG 
ATCCCCAACA TCGAGAAACT CCGATCCTGT GGAACACATG CACCATACAT GAGGCCTATG 
TACCCTACAA AAACCTACCC AAATTTGTAT ACAATTACAA CGGGGCTTTA TCCTGAATCG 
CATGGGATTG TGGGTAACTC CATTCATGAT CCAAGCTTTG ATGCCAACTT TAACTTCCGT 
GGGAAAGAGA AGCTTAATCA TCGATGGTGG GGAGGCCAGC CTATCTGGAT TACTGCAATG 
AAACAGGGAG TAAAGGCTGG TTCATTTTTC TGGCCAGTTG CCATTCCAAT GGAAAGAAGA 
GTTCTGACCA TGTTGCAATG GCTTCATCTC CCTGATGCAG AGAGGCCATA TCTTTATGCA 
ATGCATTCTG AACAGCTGGA TTCATATGGC CACAAACTAG GACCCCACAG CACAGAGCTT 
AACAGTGCAC TAAGGGATGT TGATAAGGTG ATTGGACAGC TGATGAATGG CCTGAAACAG 
ATGAAACTAC ACCGTTGTAT CAATATCATT TTGGTTGGAG ACCATGGTAT GGAGGAAGCT 
CATTGTGACA AAACAGAGTT CCTAAGTTCT TATATGTCCA ACACTGAGGA CCTCATCCTA 
ATTCCTGGCT CTCTGGGAAG AATCCGAGCC CGAAATCCCA ACAACTCTAA ATTTGATGCA 
AAAGCAGTTG TGGCAAACCT AACATGTAAA AAGCCAGACC AGCACTTTAA GCCATACTTA 
AAGCAGCATC TTCCCAAACG TTTGCACTAT GCCAATAACG ACAGAATTGA GGAGATTCAT 
TTAATGGTTG AAAGGAAATG GCACATTGCA CGGAAAATCA TGAAAACAAA AAGGAATCAT 
GAGAAATGTG GGTTTGCTGG TGATCACGGA TATGACAATA AGATCAACAG CATGCAGACA 
ATTTTCCTTG GATATGGACC AGCTTTCAAA TTCAAAACCA AAATTCCACC ATTCGAGAAT 
ATTGAGCTTT ATAATATCAT GTGTGATCTC CTTGGCTTGA AGCCAGCCCC TAACAATGGT 
ACACATGGTA GTCTTAACCA GCTCCTGAGG ACCCCTGTAT ACATACCCAA CATGCCTGAA 
GAAGTGACCA AGCCAAACCC TGCAGGACCA GTCATAGCTA TTAATGATGA CCTGGGCTGC 
ACTTGTGAAG ACAAGAACAA AGTGGACGAG CTCAATCAAC GACTGAGACA AGTTATTGAT 
GACAACAAGA ATCTTCCATA TGGGCGTCCA GCTGTTATGT TTCGCACCAA ATACTGCATT 
CTTCATCATA CTGACTACAT TAGTGGATAC AGTGAAGCTT TACATATGCC TCTTTGGACA 
TCTTATACCG TTTCCAAGCA GGTAGACTTT ACCCCTCTGA CTGATGCTCT GAGCAACTGC 
ATTCGACCTG ATAGTAGAGT TCCTACAGCT TACAGCCAGT CCTGCTCAAA CTACCGCACA 
GAAAAACAGA TCTCCTACAG TTTCCTCTAT CCACCTCAGT TGTCCTCCAC TCAGGAAGCG 
AGATATGATG CTGTTCTCAT TACCAACACT GTACCAATGT ATGCAGCATT CAAACGAGTG 
TGGAGTTATT TTCAGAAATC ACTTGTGAAA CGTTACGCTA GTGAGAAAAA TGGTGTGAAT 
GTTGTCACCG GGCCCATTTT CGACTATGAC TACAATGGTC TTAGAGATTC TGCAGAGACA 
ATAAAACAGT TTGTCAGTGG CTCCGTACAA ATCCCAACTC ATTACTTTGT TGTGGTTACA 
AGCTGCCTCG ATTACACACA GACCGTTGAC TCCTGTGTTG GTCCTCTCAG TGTGTTCTCC 
TTCATCCTTC CACACCGATC AGATAATGAA GAGACCTGCA ATAGTTCAGA AGATGAGTCA 
AAATGGGTGG AGGAACTGAT GAAGACTCAT ACAGCCCGCC TGCGAGATGT GGAGTTACTC 
ACAGGACTGG ACTTTTATCG CCGCACCAGC AGGACCTATG AGGAGATTCT CGCTCTGAAA 
ACGTACTTGC ACACATACGA AAGTGAGATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

enpp2 ( XM_009292082.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290357.1 Danio rerio ectonucleotide pyrophosphatase/phosphodiesterase 2 (enpp2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa21160
Clone ID Related Accession (Same CDS sequence)	XM_009292082.2 , XM_009292082.3
Accession Version	XM_009292082.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ectonucleotide pyrophosphatase/phosphodiesterase family member 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292082.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCAAC TTAAATGGGT ATTCTCCGTT ATGTGGCTTT TCTCACGGTT AACAGTGTGT 
AAGACTTATG TGGTCAGACG GTCCGGAAAG GCTGCATCAC CAGACGAATC GCTCTCAAAA 
AGTTTCTCTG ACCAGCCATT TACCAGCCTG GCAGGTTCTT GCAAAAAACG GTGTTTTGAG 
CTAGTGGAAG CTGATCCACC AAATTGTCGC TGTGACAACC TGTGTAAAAC ATACAATATG 
TGTTGCTCAG ACTTTGATGA CCACTGTCTA AAAACTGCAG GGGGTTTTGA ATGTAGTAAA 
GAGCGTTGTG GTGAAAATCG TAATGAGCAG CATGCCTGCC ACTGCTCAGA GGACTGCATG 
GCCAAAGGAG ACTGCTGCAC CAACTACAGG TCTCTTTGCA AAGGCGATGT TCCTTGGTTG 
CAAGAAGAGT GTGAAGAGAT TAAGAACCAC GAGTGTCCTG CTGGGTTTGT TCGACCTCCT 
GTCATCATGT TGTCTGTTGA TGGCTTTCGT GCATCTTACA TGAAAAGAGG AGGCACGGTG 
ATCCCCAACA TCGAGAAACT CCGATCCTGT GGAACACATG CACCATACAT GAGGCCTATG 
TATCCTACAA AAACCTACCC AAATTTGTAT ACAATTACAA CGGGGCTTTA TCCTGAATCG 
CATGGGATTG TGGGTAACTC CATTCATGAT CCAAGCTTTG ATGCCAACTT TAACTTCCGT 
GGGAAAGAGA AGCTTAATCA TCGATGGTGG GGAGGCCAGC CTATCTGGAT TACTGCAATG 
AAACAGGGAG TAAAGGCTGG TTCATTTTTC TGGCCAGTTG CCATTCCAAT GGAAAGAAGA 
GTTCTGACCA TGTTGCAATG GCTTCATCTC CCCGATGCAG AGAGGCCATA TCTTTATGCA 
ATGCATTCTG AACAGCTGGA TTCATATGGC CACAAACTAG GACCCCACAG CACAGAGCTT 
AACAGTGCAC TAAGGGATGT TGATAAGGTG ATTGGACAGC TGATGAATGG CCTGAAACAG 
ATGAAACTAC ACCGTTGTAT CAATATCATT TTGGTTGGAG ACCATGGTAT GGAGGAAGCT 
CATTGTGACA AAACAGAGTT CCTAAGTTCT TATATGTCCA ACACTGAGGA CCTCATCCTA 
ATTCCTGGCT CTCTGGGAAG AATCCGAGCC CGAAATCCCA ACAACTCTAA ATTTGATGCA 
AAAGCAGTTG TGGCAAACCT AACATGTAAA AAGCCAGACC AGCACTTTAA GCCATACTTA 
AAGCAGCATC TTCCCAAACG TTTGCACTAT GCCAATAACG ACAGAATTGA GGAGATTCAT 
TTAATGGTTG AAAGGAAATG GCACATTGCA CGGAAAATCA TGAAAACAAA AAGGAATCAT 
GAGAAATGTG GGTTTGCTGG TGATCACGGA TATGACAATA AGATCAACAG CATGCAGACA 
ATTTTCCTTG GATATGGACC AGCTTTCAAA TTCAAAACCA AAATTCCACC ATTCGAGAAT 
ATTGAGCTTT ATAATATCAT GTGTGATCTC CTTGGCTTGA AGCCAGCCCC TAACAATGGT 
ACACATGGTA GTCTTAACCA GCTCCTGAGG ACCCCTGTAT ACATACCCAA CATGCCTGAA 
GAAGTGACCA AGCCAAACCC TGCAGGACCA GTCATAGCTA TTAATGATGA CCTGGGCTGC 
ACTTGTGAAG ACAAGGTCAG CAATAAAAAC AAAGTGGACG AGCTCAATCA ACGACTGAGA 
CAAGTTATTG ATGACAACAA GAATCTTCCA TATGGGCGTC CAGCTGTTAT GTTTCGCACC 
AAATACTGCA TTCTTCATCA TACTGACTAC ATTAGTGGAT ACAGTGAAGC TTTACATATG 
CCTCTTTGGA CATCTTATAC CGTTTCCAAG CAGGTAGACT TTACCCCTCT GACTGATGCT 
CTGAGCAACT GCATTCGACC TGATAGTAGA GTTCCTACAG CTTACAGCCA GTCCTGCTCA 
AACTACCGCA CAGAAAAACA GATCTCCTAC AGTTTCCTCT ATCCACCTCA GTTGTCCTCC 
ACTCAGGAAG CAAGATATGA TGCTGTTCTC ATTACCAACA CTGTACCAAT GTATGCAGCA 
TTCAAACGAG TGTGGAGTTA TTTTCAGAAA TCACTTGTGA AACGTTACGC TAGTGAGAAA 
AATGGTGTGA ATGTGGTCAC CGGGCCCATT TTCGACTATG ACTACAATGG TCTTAGAGAT 
TCTGCAGAGA CAATAAAACA GTTTGTCAGT GGCTCCGTAC AAATCCCAAC TCATTACTTT 
GTTGTGGTTA CAAGCTGCCT CGATTACACA CAGACCGTTG ACTCCTGTGT TGGTCCTCTC 
AGTGTGTTCT CCTTCATCCT TCCACACCGA TCAGATAATG AAGAGACCTG CAATAGTTCA 
GAAGATGAGT CAAAGTGGGT GGAGGAACTG ATGAAGACTC ATACAGCCCG CCTGCGGGAT 
GTGGAGTTAC TCACAGGACT GGACTTTTAT CGCCGTACCA GCAGGACCTA TGAGGAGATT 
CTCGCTCTGA AAACGTACTT GCACACATAC GAAAGTGAGA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290357.1
CDS	70..2634
Translation	MLQLKWVFSVMWLFSRLTVCKTYVVRRSGKAASPDESLSKSFSDQPFTSLAGSCKKRCFELVEADPPNCRCDNLCKTYNMCCSDFDDHCLKTAGGFECSKERCGENRNEQHACHCSEDCMAKGDCCTNYRSLCKGDVPWLQEECEEIKNHECPAGFVRPPVIMLSVDGFRASYMKRGGTVIPNIEKLRSCGTHAPYMRPMYPTKTYPNLYTITTGLYPESHGIVGNSIHDPSFDANFNFRGKEKLNHRWWGGQPIWITAMKQGVKAGSFFWPVAIPMERRVLTMLQWLHLPDAERPYLYAMHSEQLDSYGHKLGPHSTELNSALRDVDKVIGQLMNGLKQMKLHRCINIILVGDHGMEEAHCDKTEFLSSYMSNTEDLILIPGSLGRIRARNPNNSKFDAKAVVANLTCKKPDQHFKPYLKQHLPKRLHYANNDRIEEIHLMVERKWHIARKIMKTKRNHEKCGFAGDHGYDNKINSMQTIFLGYGPAFKFKTKIPPFENIELYNIMCDLLGLKPAPNNGTHGSLNQLLRTPVYIPNMPEEVTKPNPAGPVIAINDDLGCTCEDKVSNKNKVDELNQRLRQVIDDNKNLPYGRPAVMFRTKYCILHHTDYISGYSEALHMPLWTSYTVSKQVDFTPLTDALSNCIRPDSRVPTAYSQSCSNYRTEKQISYSFLYPPQLSSTQEARYDAVLITNTVPMYAAFKRVWSYFQKSLVKRYASEKNGVNVVTGPIFDYDYNGLRDSAETIKQFVSGSVQIPTHYFVVVTSCLDYTQTVDSCVGPLSVFSFILPHRSDNEETCNSSEDESKWVEELMKTHTARLRDVELLTGLDFYRRTSRTYEEILALKTYLHTYESEI

Target ORF information:

RefSeq Version	XM_009292082.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ectonucleotide pyrophosphatase/phosphodiesterase 2 (enpp2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292082.2

ORF Insert Sequence:

ATGCTGCAAC TTAAATGGGT ATTCTCCGTT ATGTGGCTTT TCTCACGGTT AACAGTGTGT 
AAGACTTATG TGGTCAGACG GTCCGGAAAG GCTGCATCAC CAGACGAATC GCTCTCAAAA 
AGTTTCTCTG ACCAGCCATT TACCAGCCTG GCAGGTTCTT GCAAAAAACG GTGTTTTGAG 
CTAGTGGAAG CTGATCCACC AAATTGTCGC TGTGACAACC TGTGTAAAAC ATACAATATG 
TGTTGCTCAG ACTTTGATGA CCACTGTCTA AAAACTGCAG GGGGTTTTGA ATGTAGTAAA 
GAGCGTTGTG GTGAAAATCG TAATGAGCAG CATGCCTGCC ACTGCTCAGA GGACTGCATG 
GCCAAAGGAG ACTGCTGCAC CAACTACAGG TCTCTTTGCA AAGGCGATGT TCCTTGGTTG 
CAAGAAGAGT GTGAAGAGAT TAAGAACCAC GAGTGTCCTG CTGGGTTTGT TCGACCTCCT 
GTCATCATGT TGTCTGTTGA TGGCTTTCGT GCATCTTACA TGAAAAGAGG AGGCACGGTG 
ATCCCCAACA TCGAGAAACT CCGATCCTGT GGAACACATG CACCATACAT GAGGCCTATG 
TATCCTACAA AAACCTACCC AAATTTGTAT ACAATTACAA CGGGGCTTTA TCCTGAATCG 
CATGGGATTG TGGGTAACTC CATTCATGAT CCAAGCTTTG ATGCCAACTT TAACTTCCGT 
GGGAAAGAGA AGCTTAATCA TCGATGGTGG GGAGGCCAGC CTATCTGGAT TACTGCAATG 
AAACAGGGAG TAAAGGCTGG TTCATTTTTC TGGCCAGTTG CCATTCCAAT GGAAAGAAGA 
GTTCTGACCA TGTTGCAATG GCTTCATCTC CCCGATGCAG AGAGGCCATA TCTTTATGCA 
ATGCATTCTG AACAGCTGGA TTCATATGGC CACAAACTAG GACCCCACAG CACAGAGCTT 
AACAGTGCAC TAAGGGATGT TGATAAGGTG ATTGGACAGC TGATGAATGG CCTGAAACAG 
ATGAAACTAC ACCGTTGTAT CAATATCATT TTGGTTGGAG ACCATGGTAT GGAGGAAGCT 
CATTGTGACA AAACAGAGTT CCTAAGTTCT TATATGTCCA ACACTGAGGA CCTCATCCTA 
ATTCCTGGCT CTCTGGGAAG AATCCGAGCC CGAAATCCCA ACAACTCTAA ATTTGATGCA 
AAAGCAGTTG TGGCAAACCT AACATGTAAA AAGCCAGACC AGCACTTTAA GCCATACTTA 
AAGCAGCATC TTCCCAAACG TTTGCACTAT GCCAATAACG ACAGAATTGA GGAGATTCAT 
TTAATGGTTG AAAGGAAATG GCACATTGCA CGGAAAATCA TGAAAACAAA AAGGAATCAT 
GAGAAATGTG GGTTTGCTGG TGATCACGGA TATGACAATA AGATCAACAG CATGCAGACA 
ATTTTCCTTG GATATGGACC AGCTTTCAAA TTCAAAACCA AAATTCCACC ATTCGAGAAT 
ATTGAGCTTT ATAATATCAT GTGTGATCTC CTTGGCTTGA AGCCAGCCCC TAACAATGGT 
ACACATGGTA GTCTTAACCA GCTCCTGAGG ACCCCTGTAT ACATACCCAA CATGCCTGAA 
GAAGTGACCA AGCCAAACCC TGCAGGACCA GTCATAGCTA TTAATGATGA CCTGGGCTGC 
ACTTGTGAAG ACAAGGTCAG CAATAAAAAC AAAGTGGACG AGCTCAATCA ACGACTGAGA 
CAAGTTATTG ATGACAACAA GAATCTTCCA TATGGGCGTC CAGCTGTTAT GTTTCGCACC 
AAATACTGCA TTCTTCATCA TACTGACTAC ATTAGTGGAT ACAGTGAAGC TTTACATATG 
CCTCTTTGGA CATCTTATAC CGTTTCCAAG CAGGTAGACT TTACCCCTCT GACTGATGCT 
CTGAGCAACT GCATTCGACC TGATAGTAGA GTTCCTACAG CTTACAGCCA GTCCTGCTCA 
AACTACCGCA CAGAAAAACA GATCTCCTAC AGTTTCCTCT ATCCACCTCA GTTGTCCTCC 
ACTCAGGAAG CAAGATATGA TGCTGTTCTC ATTACCAACA CTGTACCAAT GTATGCAGCA 
TTCAAACGAG TGTGGAGTTA TTTTCAGAAA TCACTTGTGA AACGTTACGC TAGTGAGAAA 
AATGGTGTGA ATGTGGTCAC CGGGCCCATT TTCGACTATG ACTACAATGG TCTTAGAGAT 
TCTGCAGAGA CAATAAAACA GTTTGTCAGT GGCTCCGTAC AAATCCCAAC TCATTACTTT 
GTTGTGGTTA CAAGCTGCCT CGATTACACA CAGACCGTTG ACTCCTGTGT TGGTCCTCTC 
AGTGTGTTCT CCTTCATCCT TCCACACCGA TCAGATAATG AAGAGACCTG CAATAGTTCA 
GAAGATGAGT CAAAGTGGGT GGAGGAACTG ATGAAGACTC ATACAGCCCG CCTGCGGGAT 
GTGGAGTTAC TCACAGGACT GGACTTTTAT CGCCGTACCA GCAGGACCTA TGAGGAGATT 
CTCGCTCTGA AAACGTACTT GCACACATAC GAAAGTGAGA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

enpp2 ( XM_009292082.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290357.1 Danio rerio ectonucleotide pyrophosphatase/phosphodiesterase 2 (enpp2), transcript variant X1, mRNA.

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CloneID	ODa21160
Clone ID Related Accession (Same CDS sequence)	XM_009292082.2 , XM_009292082.3
Accession Version	XM_009292082.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ectonucleotide pyrophosphatase/phosphodiesterase family member 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009292082.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCAAC TTAAATGGGT ATTCTCCGTT ATGTGGCTTT TCTCACGGTT AACAGTGTGT 
AAGACTTATG TGGTCAGACG GTCCGGAAAG GCTGCATCAC CAGACGAATC GCTCTCAAAA 
AGTTTCTCTG ACCAGCCATT TACCAGCCTG GCAGGTTCTT GCAAAAAACG GTGTTTTGAG 
CTAGTGGAAG CTGATCCACC AAATTGTCGC TGTGACAACC TGTGTAAAAC ATACAATATG 
TGTTGCTCAG ACTTTGATGA CCACTGTCTA AAAACTGCAG GGGGTTTTGA ATGTAGTAAA 
GAGCGTTGTG GTGAAAATCG TAATGAGCAG CATGCCTGCC ACTGCTCAGA GGACTGCATG 
GCCAAAGGAG ACTGCTGCAC CAACTACAGG TCTCTTTGCA AAGGCGATGT TCCTTGGTTG 
CAAGAAGAGT GTGAAGAGAT TAAGAACCAC GAGTGTCCTG CTGGGTTTGT TCGACCTCCT 
GTCATCATGT TGTCTGTTGA TGGCTTTCGT GCATCTTACA TGAAAAGAGG AGGCACGGTG 
ATCCCCAACA TCGAGAAACT CCGATCCTGT GGAACACATG CACCATACAT GAGGCCTATG 
TATCCTACAA AAACCTACCC AAATTTGTAT ACAATTACAA CGGGGCTTTA TCCTGAATCG 
CATGGGATTG TGGGTAACTC CATTCATGAT CCAAGCTTTG ATGCCAACTT TAACTTCCGT 
GGGAAAGAGA AGCTTAATCA TCGATGGTGG GGAGGCCAGC CTATCTGGAT TACTGCAATG 
AAACAGGGAG TAAAGGCTGG TTCATTTTTC TGGCCAGTTG CCATTCCAAT GGAAAGAAGA 
GTTCTGACCA TGTTGCAATG GCTTCATCTC CCCGATGCAG AGAGGCCATA TCTTTATGCA 
ATGCATTCTG AACAGCTGGA TTCATATGGC CACAAACTAG GACCCCACAG CACAGAGCTT 
AACAGTGCAC TAAGGGATGT TGATAAGGTG ATTGGACAGC TGATGAATGG CCTGAAACAG 
ATGAAACTAC ACCGTTGTAT CAATATCATT TTGGTTGGAG ACCATGGTAT GGAGGAAGCT 
CATTGTGACA AAACAGAGTT CCTAAGTTCT TATATGTCCA ACACTGAGGA CCTCATCCTA 
ATTCCTGGCT CTCTGGGAAG AATCCGAGCC CGAAATCCCA ACAACTCTAA ATTTGATGCA 
AAAGCAGTTG TGGCAAACCT AACATGTAAA AAGCCAGACC AGCACTTTAA GCCATACTTA 
AAGCAGCATC TTCCCAAACG TTTGCACTAT GCCAATAACG ACAGAATTGA GGAGATTCAT 
TTAATGGTTG AAAGGAAATG GCACATTGCA CGGAAAATCA TGAAAACAAA AAGGAATCAT 
GAGAAATGTG GGTTTGCTGG TGATCACGGA TATGACAATA AGATCAACAG CATGCAGACA 
ATTTTCCTTG GATATGGACC AGCTTTCAAA TTCAAAACCA AAATTCCACC ATTCGAGAAT 
ATTGAGCTTT ATAATATCAT GTGTGATCTC CTTGGCTTGA AGCCAGCCCC TAACAATGGT 
ACACATGGTA GTCTTAACCA GCTCCTGAGG ACCCCTGTAT ACATACCCAA CATGCCTGAA 
GAAGTGACCA AGCCAAACCC TGCAGGACCA GTCATAGCTA TTAATGATGA CCTGGGCTGC 
ACTTGTGAAG ACAAGGTCAG CAATAAAAAC AAAGTGGACG AGCTCAATCA ACGACTGAGA 
CAAGTTATTG ATGACAACAA GAATCTTCCA TATGGGCGTC CAGCTGTTAT GTTTCGCACC 
AAATACTGCA TTCTTCATCA TACTGACTAC ATTAGTGGAT ACAGTGAAGC TTTACATATG 
CCTCTTTGGA CATCTTATAC CGTTTCCAAG CAGGTAGACT TTACCCCTCT GACTGATGCT 
CTGAGCAACT GCATTCGACC TGATAGTAGA GTTCCTACAG CTTACAGCCA GTCCTGCTCA 
AACTACCGCA CAGAAAAACA GATCTCCTAC AGTTTCCTCT ATCCACCTCA GTTGTCCTCC 
ACTCAGGAAG CAAGATATGA TGCTGTTCTC ATTACCAACA CTGTACCAAT GTATGCAGCA 
TTCAAACGAG TGTGGAGTTA TTTTCAGAAA TCACTTGTGA AACGTTACGC TAGTGAGAAA 
AATGGTGTGA ATGTGGTCAC CGGGCCCATT TTCGACTATG ACTACAATGG TCTTAGAGAT 
TCTGCAGAGA CAATAAAACA GTTTGTCAGT GGCTCCGTAC AAATCCCAAC TCATTACTTT 
GTTGTGGTTA CAAGCTGCCT CGATTACACA CAGACCGTTG ACTCCTGTGT TGGTCCTCTC 
AGTGTGTTCT CCTTCATCCT TCCACACCGA TCAGATAATG AAGAGACCTG CAATAGTTCA 
GAAGATGAGT CAAAGTGGGT GGAGGAACTG ATGAAGACTC ATACAGCCCG CCTGCGGGAT 
GTGGAGTTAC TCACAGGACT GGACTTTTAT CGCCGTACCA GCAGGACCTA TGAGGAGATT 
CTCGCTCTGA AAACGTACTT GCACACATAC GAAAGTGAGA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290357.1
CDS	71..2635
Translation	MLQLKWVFSVMWLFSRLTVCKTYVVRRSGKAASPDESLSKSFSDQPFTSLAGSCKKRCFELVEADPPNCRCDNLCKTYNMCCSDFDDHCLKTAGGFECSKERCGENRNEQHACHCSEDCMAKGDCCTNYRSLCKGDVPWLQEECEEIKNHECPAGFVRPPVIMLSVDGFRASYMKRGGTVIPNIEKLRSCGTHAPYMRPMYPTKTYPNLYTITTGLYPESHGIVGNSIHDPSFDANFNFRGKEKLNHRWWGGQPIWITAMKQGVKAGSFFWPVAIPMERRVLTMLQWLHLPDAERPYLYAMHSEQLDSYGHKLGPHSTELNSALRDVDKVIGQLMNGLKQMKLHRCINIILVGDHGMEEAHCDKTEFLSSYMSNTEDLILIPGSLGRIRARNPNNSKFDAKAVVANLTCKKPDQHFKPYLKQHLPKRLHYANNDRIEEIHLMVERKWHIARKIMKTKRNHEKCGFAGDHGYDNKINSMQTIFLGYGPAFKFKTKIPPFENIELYNIMCDLLGLKPAPNNGTHGSLNQLLRTPVYIPNMPEEVTKPNPAGPVIAINDDLGCTCEDKVSNKNKVDELNQRLRQVIDDNKNLPYGRPAVMFRTKYCILHHTDYISGYSEALHMPLWTSYTVSKQVDFTPLTDALSNCIRPDSRVPTAYSQSCSNYRTEKQISYSFLYPPQLSSTQEARYDAVLITNTVPMYAAFKRVWSYFQKSLVKRYASEKNGVNVVTGPIFDYDYNGLRDSAETIKQFVSGSVQIPTHYFVVVTSCLDYTQTVDSCVGPLSVFSFILPHRSDNEETCNSSEDESKWVEELMKTHTARLRDVELLTGLDFYRRTSRTYEEILALKTYLHTYESEI

Target ORF information:

RefSeq Version	XM_009292082.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ectonucleotide pyrophosphatase/phosphodiesterase 2 (enpp2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292082.3

ORF Insert Sequence:

ATGCTGCAAC TTAAATGGGT ATTCTCCGTT ATGTGGCTTT TCTCACGGTT AACAGTGTGT 
AAGACTTATG TGGTCAGACG GTCCGGAAAG GCTGCATCAC CAGACGAATC GCTCTCAAAA 
AGTTTCTCTG ACCAGCCATT TACCAGCCTG GCAGGTTCTT GCAAAAAACG GTGTTTTGAG 
CTAGTGGAAG CTGATCCACC AAATTGTCGC TGTGACAACC TGTGTAAAAC ATACAATATG 
TGTTGCTCAG ACTTTGATGA CCACTGTCTA AAAACTGCAG GGGGTTTTGA ATGTAGTAAA 
GAGCGTTGTG GTGAAAATCG TAATGAGCAG CATGCCTGCC ACTGCTCAGA GGACTGCATG 
GCCAAAGGAG ACTGCTGCAC CAACTACAGG TCTCTTTGCA AAGGCGATGT TCCTTGGTTG 
CAAGAAGAGT GTGAAGAGAT TAAGAACCAC GAGTGTCCTG CTGGGTTTGT TCGACCTCCT 
GTCATCATGT TGTCTGTTGA TGGCTTTCGT GCATCTTACA TGAAAAGAGG AGGCACGGTG 
ATCCCCAACA TCGAGAAACT CCGATCCTGT GGAACACATG CACCATACAT GAGGCCTATG 
TATCCTACAA AAACCTACCC AAATTTGTAT ACAATTACAA CGGGGCTTTA TCCTGAATCG 
CATGGGATTG TGGGTAACTC CATTCATGAT CCAAGCTTTG ATGCCAACTT TAACTTCCGT 
GGGAAAGAGA AGCTTAATCA TCGATGGTGG GGAGGCCAGC CTATCTGGAT TACTGCAATG 
AAACAGGGAG TAAAGGCTGG TTCATTTTTC TGGCCAGTTG CCATTCCAAT GGAAAGAAGA 
GTTCTGACCA TGTTGCAATG GCTTCATCTC CCCGATGCAG AGAGGCCATA TCTTTATGCA 
ATGCATTCTG AACAGCTGGA TTCATATGGC CACAAACTAG GACCCCACAG CACAGAGCTT 
AACAGTGCAC TAAGGGATGT TGATAAGGTG ATTGGACAGC TGATGAATGG CCTGAAACAG 
ATGAAACTAC ACCGTTGTAT CAATATCATT TTGGTTGGAG ACCATGGTAT GGAGGAAGCT 
CATTGTGACA AAACAGAGTT CCTAAGTTCT TATATGTCCA ACACTGAGGA CCTCATCCTA 
ATTCCTGGCT CTCTGGGAAG AATCCGAGCC CGAAATCCCA ACAACTCTAA ATTTGATGCA 
AAAGCAGTTG TGGCAAACCT AACATGTAAA AAGCCAGACC AGCACTTTAA GCCATACTTA 
AAGCAGCATC TTCCCAAACG TTTGCACTAT GCCAATAACG ACAGAATTGA GGAGATTCAT 
TTAATGGTTG AAAGGAAATG GCACATTGCA CGGAAAATCA TGAAAACAAA AAGGAATCAT 
GAGAAATGTG GGTTTGCTGG TGATCACGGA TATGACAATA AGATCAACAG CATGCAGACA 
ATTTTCCTTG GATATGGACC AGCTTTCAAA TTCAAAACCA AAATTCCACC ATTCGAGAAT 
ATTGAGCTTT ATAATATCAT GTGTGATCTC CTTGGCTTGA AGCCAGCCCC TAACAATGGT 
ACACATGGTA GTCTTAACCA GCTCCTGAGG ACCCCTGTAT ACATACCCAA CATGCCTGAA 
GAAGTGACCA AGCCAAACCC TGCAGGACCA GTCATAGCTA TTAATGATGA CCTGGGCTGC 
ACTTGTGAAG ACAAGGTCAG CAATAAAAAC AAAGTGGACG AGCTCAATCA ACGACTGAGA 
CAAGTTATTG ATGACAACAA GAATCTTCCA TATGGGCGTC CAGCTGTTAT GTTTCGCACC 
AAATACTGCA TTCTTCATCA TACTGACTAC ATTAGTGGAT ACAGTGAAGC TTTACATATG 
CCTCTTTGGA CATCTTATAC CGTTTCCAAG CAGGTAGACT TTACCCCTCT GACTGATGCT 
CTGAGCAACT GCATTCGACC TGATAGTAGA GTTCCTACAG CTTACAGCCA GTCCTGCTCA 
AACTACCGCA CAGAAAAACA GATCTCCTAC AGTTTCCTCT ATCCACCTCA GTTGTCCTCC 
ACTCAGGAAG CAAGATATGA TGCTGTTCTC ATTACCAACA CTGTACCAAT GTATGCAGCA 
TTCAAACGAG TGTGGAGTTA TTTTCAGAAA TCACTTGTGA AACGTTACGC TAGTGAGAAA 
AATGGTGTGA ATGTGGTCAC CGGGCCCATT TTCGACTATG ACTACAATGG TCTTAGAGAT 
TCTGCAGAGA CAATAAAACA GTTTGTCAGT GGCTCCGTAC AAATCCCAAC TCATTACTTT 
GTTGTGGTTA CAAGCTGCCT CGATTACACA CAGACCGTTG ACTCCTGTGT TGGTCCTCTC 
AGTGTGTTCT CCTTCATCCT TCCACACCGA TCAGATAATG AAGAGACCTG CAATAGTTCA 
GAAGATGAGT CAAAGTGGGT GGAGGAACTG ATGAAGACTC ATACAGCCCG CCTGCGGGAT 
GTGGAGTTAC TCACAGGACT GGACTTTTAT CGCCGTACCA GCAGGACCTA TGAGGAGATT 
CTCGCTCTGA AAACGTACTT GCACACATAC GAAAGTGAGA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An Adenosine Receptor for Olfaction in Fish.
Current biology : CB27(10)1437-1447.e4(2017 May)
Wakisaka N,Miyasaka N,Koide T,Masuda M,Hiraki-Kajiyama T,Yoshihara Y

Role of ectonucleotide pyrophosphatase/phosphodiesterase 2 in the midline axis formation of zebrafish.
Scientific reports637678(2016 Nov)
Frisca F,Colquhoun D,Goldshmit Y,?nk? ML,P?bay A,Kaslin J

Autotaxin-Lysophosphatidic Acid Axis Acts Downstream of Apoprotein B Lipoproteins in Endothelial Cells.
Arteriosclerosis, thrombosis, and vascular biology36(10)2058-67(2016 Oct)
Gibbs-Bar L,Tempelhof H,Ben-Hamo R,Ely Y,Brandis A,Hofi R,Almog G,Braun T,Feldmesser E,Efroni S,Yaniv K

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

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enpp2 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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