cbl cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cbl
Entrez Gene ID	492364
Full Name	Cbl proto-oncogene, E3 ubiquitin protein ligase
Synonyms	wu:fd16a12,zgc:92560
General protein information	Preferred Names Cbl proto-oncogene, E3 ubiquitin protein ligase Names E3 ubiquitin-protein ligase CBL Cas-Br-M (murine) ecotropic retroviral transforming sequence
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005157579.3	XP_005157636.1	E3 ubiquitin-protein ligase CBL isoform X1
NM_001007330.1	NP_001007331.1	E3 ubiquitin-protein ligase CBL
XM_005157579.4	XP_005157636.1	E3 ubiquitin-protein ligase CBL isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	CBL	NP_989539.1
	CBL	XP_001104812.1
Xenopus tropicalis (tropical clawed frog)	cbl	NP_001122110.1
Pan troglodytes (chimpanzee)	CBL	XP_001165648.1
Mus musculus (house mouse)	Cbl	NP_031645.2
Homo sapiens (human)	CBL	NP_005179.2
Canis lupus familiaris (dog)	CBL	XP_546487.3
Bos taurus (cattle)	CBL	XP_002693128.2
Rattus norvegicus (Norway rat)	Cbl	XP_576396.3
Danio rerio (zebrafish)	cbl	NP_001007331.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

A point mutation of zebrafish c-cbl gene in the ring finger domain produces a phenotype mimicking human myeloproliferative disease.
Peng X, Dong M, Ma L, Jia XE, Mao J, Jin C, Chen Y, Gao L, Liu X, Ma K, Wang L, Du T, Jin Y, Huang Q, Li K, Zon LI, Liu T, Deng M, Zhou Y, Xi X, Zhou Y, Chen S
Leukemia29(12)2355-65(2015 Dec)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The c-Cbl ubiquitin ligase regulates nuclear ?-catenin and angiogenesis by its tyrosine phosphorylation mediated through the Wnt signaling pathway.
Shivanna S, Harrold I, Shashar M, Meyer R, Kiang C, Francis J, Zhao Q, Feng H, Edelman ER, Rahimi N, Chitalia VC
The Journal of biological chemistry290(20)12537-46(2015 May)

Somatic mutagenesis with a Sleeping Beauty transposon system leads to solid tumor formation in zebrafish.
McGrail M, Hatler JM, Kuang X, Liao HK, Nannapaneni K, Watt KE, Uhl JD, Largaespada DA, Vollbrecht E, Scheetz TE, Dupuy AJ, Hostetter JM, Essner JJ
PloS one6(4)e18826(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

novel mechanism for the regulation of active nuclear beta-catenin by c-Cbl and its critical role in angiogenesis.
Title: The c-Cbl ubiquitin ligase regulates nuclear ?-catenin and angiogenesis by its tyrosine phosphorylation mediated through the Wnt signaling pathway.

The following cbl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cbl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa02468	NM_001007330.1 Latest version!	Danio rerio Cbl proto-oncogene, E3 ubiquitin protein ligase (cbl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa20476	XM_005157579.4 Latest version!	Danio rerio Cbl proto-oncogene, E3 ubiquitin protein ligase (cbl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa20476	XM_005157579.3	Danio rerio Cbl proto-oncogene, E3 ubiquitin protein ligase (cbl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cbl ( NM_001007330.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001007331.1 Danio rerio Cbl proto-oncogene, E3 ubiquitin protein ligase (cbl), mRNA.

Price & Availability↑

CloneID	ODa02468
Clone ID Related Accession (Same CDS sequence)	NM_001007330.1
Accession Version	NM_001007330.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase CBL
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC085671.1. ##Evidence-Data-START## Transcript exon combination :: BC085671.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGGAA ATCTGAAGAA AGGTGGGGGG CTTATTGGCA TGATGAAGGA CGCTTTTCAG 
CCTCACCATC ACCTCCACCA CTCCCATCAC CAGCCCGGAG CCGTGGACAA AAAGACGGTG 
GAGAAATGCT GGAAATTCAT GGATAAGGTG GTACGGTTAT GCCAGAACCC CAAATTGGCC 
TTGAAGAACA GTCCTCCGTA CATCCTAGAC TTGCTGCCAG ATACATACCA GCATCTCCGC 
ACTATCCTGT CTCGATATGA AGGCAAGATG GAAACGCTAG GCGACAACGA GTACTTCCGT 
GTTTTTATGG AGAACTTGAC CAAGAAGACC AAACAGACCA TAAGCCTGTT CAAAGATGGC 
AAAGAGCGAA TGTTCGAGGA GAACTCTCAG CCCAGACGGA ACTTAACAAA GTTGTCGCTG 
ATTTTCAGTC ACATGCTCGC TGAGCTCAAA GCCATCTTTC CTAATGGTCT GTTTCAAGGG 
GACAACTTCA GAATCACCAA AGCGGATGCC GCAGAGTTCT GGAGAAGATC GTTCGGGGAC 
AAGACCATTG TGCCTTGGAA GGTGTTCCGT CAGGCCCTGC ATGAGTTTCA CCCCATCAGC 
TCAGGCCTGG AGGCCATGGC ACTGAAATCC ACCATCGACC TCACATGCAA CGACTACATC 
TCCGTTTTTG AGTTTGACAT CTTCACCAGG CTATTCCAGC CATGGTCGTC ATTGTTGAGA 
AACTGGAACA GCCTGGCAGT GACTCATCCC GGGTACATGG CTTTTCTAAC CTATGATGAA 
GTCAAAGCTC GTCTACAAAA ATTCATCCAC AAGCCTGGCA GCTATATCTT CAGACTGAGC 
TGTACGAGGC TCGGACAGTG GGCTATCGGT TACGTCACGG CTGATGGAAA CATCTTGCAG 
ACCATTCCTC ATAACAAACC CCTCTTCCAG GCCCACATCG ATGGCTTTAG AGAAGGATTC 
TACCTTTTTC CAGATGGACG CACTCAAAAC CCAGACCTCA CAGGACTGTG TGAACCCTCA 
CCTCAAGACC ATATCAAAGT CACACAGGAG CAATATGAGC TGTACTGTGA GATGGGCTCT 
ACCTTCCAGC TGTGTAAGAT TTGTGCTGAG AATGACAAGG ATGTGAAGAT CGAGCCCTGC 
GGACACCTCA TGTGCACTTC CTGTCTTACA GCCTGGCAGG AGTCAGAAGG CCAAGGCTGC 
CCATTCTGTC GCTGTGAAAT TAAGGGTACA GAGCCCATCG TCGTTGACCC CTTTGATCCT 
AAGGATCCCA ACAGCGCTGC CTCCTATGGC TGCAGGGGCA TGTTTGGGGC TGAGGGAGCC 
CCTTCACCCA GCTACGACGA TGATGATGAT GACCGTTTAG AGGAAACTCC TTTCATGATT 
AGCAAGCTGG TCGGCTCTAA AGTGGAGAGG CCGCCCTCGC CGATGTCAAT CTCCCCTCAG 
CCCGTCGTTC CTCCAGTACC TCCTCGGCTG GATCTGCTTC CTCTCAGGCC CACCAACCCT 
CCTGGGGCCT CAAGTCCAGG GGCCACATCC AAGGCCCCTT CCCATCACAA AGACAAGCCA 
TTACCGCTGC CGCCGGCTCT AAGGGATCTG CCACCTCCAC CTCCCCCAGA CCGTCCACAC 
CCAGCTGGAG CAGACAACCG TCTCCCAAGA CGTCCTCTCC CCAGTACGCC TGGAGACCCA 
TCTCGTGACA AACTGCCCCC GGCCCCTCCT AACCGCAACA TGCCAGACTG GAACTCCCGG 
CCTGTACCCA AAGCCCCATC ACAGCCACCA GCAAGCGGAG ACACCCGTGG ATCTCGAGAG 
CTTTCCAACA GGCACTCTCT GCCTCTCCAA CTGCCCTCTA CTCTCGATTC CAGAACAGAA 
GCCCCCAGAA ACAACGGCCC TCCCAGCCTC GACCACCAGC TGAGTTTTGG ATCATCGAAT 
CCTGCATCGG ATTATGACAG TCCAAAAGTA AAGCCGTCTG CCTCGGCTAA TGCCATCTAT 
TCCTTGGCAA TACGGCCTGT TGCAGCAGCA AGAACACAGA CAGGGGAGGA AACCAATGAG 
AATGACGAGG AGTCCGAGTA CATGACTCCA TCTTCTCGAC CAGTGCTGCC CTCTGCTGCA 
GGGGAGGCTG TGCCCAGACC ACCTCCACCA CTCTCACTCA GTCCCAGAAG TATGCTCAAT 
GAATTAGAGT CAGAAAGTCC ACAGATGTAT GAAGCCATGT ACAACATCCA AGCACAGGCC 
CTCACAGCTT CTGCACCACA GGCCAGAGAC TCGGATTACT CCGAATTGCC TCCTTTGACC 
TGCTCTAATG GCCCGCGGGA TCCTGTCGGT GCATATGCTG AGGAGGAGGA GGAGGAGGAG 
AACAGCTATG ACTATCCCAA ACCTCATCTA TCTGCAGCTC TAGCCCGACG CACGCTTTCA 
GATGCTAACT CACCATCCTC CTCAGCCTTC AGCCGCCTCT CCTTTGACAG TGACCTTGGA 
GCAGCTGCAA CTGTCCTGGA CCCCTCTGAA GCCCCCGAGC GGCCTCCTAA GCCATTGCCC 
CGTCGCATCA ACTCGGATCG CAGACCCAGT CCAGTTCCCC CCGGGGCGAG TCCAGGCCCC 
AGCCAGCAGA TCAGCAGTGA GATCGAGCAC CTCTTGAGCC AGGGATACTC CCATCAGGAC 
ATCCAAAAAG CCCTAATGAT TGCACAGAAC AATATTGAAA TGGCCAAGAA CGTCCTGAGG 
GAGTTTGTGT CCATCCCTTC TACTGCACAT ATTTTAACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007331.1
CDS	283..3024
Translation	MAGNLKKGGGLIGMMKDAFQPHHHLHHSHHQPGAVDKKTVEKCWKFMDKVVRLCQNPKLALKNSPPYILDLLPDTYQHLRTILSRYEGKMETLGDNEYFRVFMENLTKKTKQTISLFKDGKERMFEENSQPRRNLTKLSLIFSHMLAELKAIFPNGLFQGDNFRITKADAAEFWRRSFGDKTIVPWKVFRQALHEFHPISSGLEAMALKSTIDLTCNDYISVFEFDIFTRLFQPWSSLLRNWNSLAVTHPGYMAFLTYDEVKARLQKFIHKPGSYIFRLSCTRLGQWAIGYVTADGNILQTIPHNKPLFQAHIDGFREGFYLFPDGRTQNPDLTGLCEPSPQDHIKVTQEQYELYCEMGSTFQLCKICAENDKDVKIEPCGHLMCTSCLTAWQESEGQGCPFCRCEIKGTEPIVVDPFDPKDPNSAASYGCRGMFGAEGAPSPSYDDDDDDRLEETPFMISKLVGSKVERPPSPMSISPQPVVPPVPPRLDLLPLRPTNPPGASSPGATSKAPSHHKDKPLPLPPALRDLPPPPPPDRPHPAGADNRLPRRPLPSTPGDPSRDKLPPAPPNRNMPDWNSRPVPKAPSQPPASGDTRGSRELSNRHSLPLQLPSTLDSRTEAPRNNGPPSLDHQLSFGSSNPASDYDSPKVKPSASANAIYSLAIRPVAAARTQTGEETNENDEESEYMTPSSRPVLPSAAGEAVPRPPPPLSLSPRSMLNELESESPQMYEAMYNIQAQALTASAPQARDSDYSELPPLTCSNGPRDPVGAYAEEEEEEENSYDYPKPHLSAALARRTLSDANSPSSSAFSRLSFDSDLGAAATVLDPSEAPERPPKPLPRRINSDRRPSPVPPGASPGPSQQISSEIEHLLSQGYSHQDIQKALMIAQNNIEMAKNVLREFVSIPSTAHILT

Target ORF information:

RefSeq Version	NM_001007330.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Cbl proto-oncogene, E3 ubiquitin protein ligase (cbl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007330.1

ORF Insert Sequence:

ATGGCAGGAA ATCTGAAGAA AGGTGGGGGG CTTATTGGCA TGATGAAGGA CGCTTTTCAG 
CCTCACCATC ACCTCCACCA CTCCCATCAC CAGCCCGGAG CCGTGGACAA AAAGACGGTG 
GAGAAATGCT GGAAATTCAT GGATAAGGTG GTACGGTTAT GCCAGAACCC CAAATTGGCC 
TTGAAGAACA GTCCTCCGTA CATCCTAGAC TTGCTGCCAG ATACATACCA GCATCTCCGC 
ACTATCCTGT CTCGATATGA AGGCAAGATG GAAACGCTAG GCGACAACGA GTACTTCCGT 
GTTTTTATGG AGAACTTGAC CAAGAAGACC AAACAGACCA TAAGCCTGTT CAAAGATGGC 
AAAGAGCGAA TGTTCGAGGA GAACTCTCAG CCCAGACGGA ACTTAACAAA GTTGTCGCTG 
ATTTTCAGTC ACATGCTCGC TGAGCTCAAA GCCATCTTTC CTAATGGTCT GTTTCAAGGG 
GACAACTTCA GAATCACCAA AGCGGATGCC GCAGAGTTCT GGAGAAGATC GTTCGGGGAC 
AAGACCATTG TGCCTTGGAA GGTGTTCCGT CAGGCCCTGC ATGAGTTTCA CCCCATCAGC 
TCAGGCCTGG AGGCCATGGC ACTGAAATCC ACCATCGACC TCACATGCAA CGACTACATC 
TCCGTTTTTG AGTTTGACAT CTTCACCAGG CTATTCCAGC CATGGTCGTC ATTGTTGAGA 
AACTGGAACA GCCTGGCAGT GACTCATCCC GGGTACATGG CTTTTCTAAC CTATGATGAA 
GTCAAAGCTC GTCTACAAAA ATTCATCCAC AAGCCTGGCA GCTATATCTT CAGACTGAGC 
TGTACGAGGC TCGGACAGTG GGCTATCGGT TACGTCACGG CTGATGGAAA CATCTTGCAG 
ACCATTCCTC ATAACAAACC CCTCTTCCAG GCCCACATCG ATGGCTTTAG AGAAGGATTC 
TACCTTTTTC CAGATGGACG CACTCAAAAC CCAGACCTCA CAGGACTGTG TGAACCCTCA 
CCTCAAGACC ATATCAAAGT CACACAGGAG CAATATGAGC TGTACTGTGA GATGGGCTCT 
ACCTTCCAGC TGTGTAAGAT TTGTGCTGAG AATGACAAGG ATGTGAAGAT CGAGCCCTGC 
GGACACCTCA TGTGCACTTC CTGTCTTACA GCCTGGCAGG AGTCAGAAGG CCAAGGCTGC 
CCATTCTGTC GCTGTGAAAT TAAGGGTACA GAGCCCATCG TCGTTGACCC CTTTGATCCT 
AAGGATCCCA ACAGCGCTGC CTCCTATGGC TGCAGGGGCA TGTTTGGGGC TGAGGGAGCC 
CCTTCACCCA GCTACGACGA TGATGATGAT GACCGTTTAG AGGAAACTCC TTTCATGATT 
AGCAAGCTGG TCGGCTCTAA AGTGGAGAGG CCGCCCTCGC CGATGTCAAT CTCCCCTCAG 
CCCGTCGTTC CTCCAGTACC TCCTCGGCTG GATCTGCTTC CTCTCAGGCC CACCAACCCT 
CCTGGGGCCT CAAGTCCAGG GGCCACATCC AAGGCCCCTT CCCATCACAA AGACAAGCCA 
TTACCGCTGC CGCCGGCTCT AAGGGATCTG CCACCTCCAC CTCCCCCAGA CCGTCCACAC 
CCAGCTGGAG CAGACAACCG TCTCCCAAGA CGTCCTCTCC CCAGTACGCC TGGAGACCCA 
TCTCGTGACA AACTGCCCCC GGCCCCTCCT AACCGCAACA TGCCAGACTG GAACTCCCGG 
CCTGTACCCA AAGCCCCATC ACAGCCACCA GCAAGCGGAG ACACCCGTGG ATCTCGAGAG 
CTTTCCAACA GGCACTCTCT GCCTCTCCAA CTGCCCTCTA CTCTCGATTC CAGAACAGAA 
GCCCCCAGAA ACAACGGCCC TCCCAGCCTC GACCACCAGC TGAGTTTTGG ATCATCGAAT 
CCTGCATCGG ATTATGACAG TCCAAAAGTA AAGCCGTCTG CCTCGGCTAA TGCCATCTAT 
TCCTTGGCAA TACGGCCTGT TGCAGCAGCA AGAACACAGA CAGGGGAGGA AACCAATGAG 
AATGACGAGG AGTCCGAGTA CATGACTCCA TCTTCTCGAC CAGTGCTGCC CTCTGCTGCA 
GGGGAGGCTG TGCCCAGACC ACCTCCACCA CTCTCACTCA GTCCCAGAAG TATGCTCAAT 
GAATTAGAGT CAGAAAGTCC ACAGATGTAT GAAGCCATGT ACAACATCCA AGCACAGGCC 
CTCACAGCTT CTGCACCACA GGCCAGAGAC TCGGATTACT CCGAATTGCC TCCTTTGACC 
TGCTCTAATG GCCCGCGGGA TCCTGTCGGT GCATATGCTG AGGAGGAGGA GGAGGAGGAG 
AACAGCTATG ACTATCCCAA ACCTCATCTA TCTGCAGCTC TAGCCCGACG CACGCTTTCA 
GATGCTAACT CACCATCCTC CTCAGCCTTC AGCCGCCTCT CCTTTGACAG TGACCTTGGA 
GCAGCTGCAA CTGTCCTGGA CCCCTCTGAA GCCCCCGAGC GGCCTCCTAA GCCATTGCCC 
CGTCGCATCA ACTCGGATCG CAGACCCAGT CCAGTTCCCC CCGGGGCGAG TCCAGGCCCC 
AGCCAGCAGA TCAGCAGTGA GATCGAGCAC CTCTTGAGCC AGGGATACTC CCATCAGGAC 
ATCCAAAAAG CCCTAATGAT TGCACAGAAC AATATTGAAA TGGCCAAGAA CGTCCTGAGG 
GAGTTTGTGT CCATCCCTTC TACTGCACAT ATTTTAACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cbl ( XM_005157579.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157636.1 Danio rerio Cbl proto-oncogene, E3 ubiquitin protein ligase (cbl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa20476
Clone ID Related Accession (Same CDS sequence)	XM_005157579.3 , XM_005157579.4
Accession Version	XM_005157579.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase CBL isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157579.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGAA ATCTGAAGAA AGGTGGGGGG CTTATTGGCA TGATGAAGGA CGCTTTTCAG 
CCTCACCATC ACCTCCACCA CTCCCATCAC CAGCCCGGAG CCGTGGACAA AAAGACGGTG 
GAGAAATGCT GGAAATTCAT GGATAAGGTG GTACGGTTAT GCCAGAACCC CAAATTGGCC 
TTGAAGAACA GTCCTCCGTA CATCCTAGAC TTGCTGCCAG ATACATACCA GCATCTCCGC 
ACTATCCTGT CTCGATATGA AGGCAAGATG GAAACGCTAG GCGACAACGA GTACTTCCGT 
GTTTTTATGG AGAACTTGAC CAAGAAGACC AAACAGACCA TAAGCCTGTT CAAAGATGGC 
AAAGAGCGAA TGTTCGAGGA GAACTCTCAG CCCAGACGGA ACTTAACAAA GTTGTCGCTG 
ATTTTCAGTC ACATGCTCGC TGAGCTCAAA GCCATCTTTC CTAATGGTCT GTTTCAAGGG 
GACAACTTCA GAATCACCAA AGCGGATGCC GCAGAGTTCT GGAGAAGATC GTTCGGGGAC 
AAGACCATTG TGCCTTGGAA GGTGTTCCGT CAGGCCCTGC ATGAGTTTCA CCCCATCAGC 
TCAGGCCTGG AGGCCATGGC ACTGAAATCC ACCATCGACC TCACATGCAA CGACTACATC 
TCCGTTTTTG AGTTTGACAT CTTCACCAGG CTATTCCAGC CATGGTCGTC ATTGTTGAGA 
AACTGGAACA GCCTGGCAGT GACTCATCCC GGGTACATGG CTTTTCTAAC CTATGATGAA 
GTCAAAGCTC GTCTACAAAA ATTCATCCAC AAGCCTGGCA GCTATATCTT CAGACTGAGC 
TGTACGAGGC TCGGACAGTG GGCTATCGGT TACGTCACGG CTGATGGAAA CATCTTGCAG 
ACCATTCCTC ATAACAAACC CCTCTTCCAG GCCCTCATCG ATGGCTTTAG AGAAGGATTC 
TACCTTTTTC CAGATGGACG CACTCAAAAC CCAGACCTCA CAGGACTGTG TGAACCCTCA 
CCTCAAGACC ATATCAAAGT CACACAGGAG CAATATGAGC TGTACTGTGA GATGGGCTCT 
ACCTTCCAGC TGTGTAAGAT TTGTGCTGAG AATGACAAGG ATGTGAAGAT CGAGCCCTGC 
GGACACCTCA TGTGCACTTC CTGTCTTACA GCCTGGCAGG AGTCAGAAGG CCAAGGCTGC 
CCATTCTGTC GCTGTGAAAT TAAGGGTACA GAGCCCATCG TCGTTGACCC CTTTGATCCT 
AAGGATCCCA ACAGCGCTGC CTCCTATGGC TGCAGGGGCA TGTTTGGGGC TGAGGGAGCC 
CCTTCACCCA GCTACGACGA TGATGATGAT GACCGTTTAG AGGAAACTCC TTTCATGATT 
AGCAAGCTGG TCGGCTCTAA AGTGGAGAGG CCGCCCTCGC CGATGTCAAT CTCCCCTCAG 
CCCGTCGTTC CTCCAGTACC TCCTCGGCTG GATCTGCTTC CTCTCAGGCC CACCAACCCT 
CCTGGGGCCT CAAGTCCAGG GGCCACATCC AAGGCCCCTT CCCATCACAA AGACAAGCCA 
TTACCGCTGC CGCCGGCTCT AAGGGATCTG CCACCTCCAC CTCCCCCAGA CCGTCCACAC 
CCAGCTGGAG CAGACAACCG TCTCCCAAGA CGTCCTCTCC CCAGTACGCC TGGAGACCCA 
TCTCGTGACA AACTGCCCCC GGCCCCTCCT AACCGCAACA TGCCAGACTG GAACTCCCGG 
CCTGTACCCA AAGCCCCATC ACAGCCACCA GCAAGCGGAG ACACCCGTGG ATCTCGAGAG 
CTTTCCAACA GGCACTCTCT GCCTCTCCAA CTGCCCTCTA CTCTCGATTC CAGAACAGAA 
GCCCCCAGAA ACAACGGCCC TCCCAGCCTC GACCACCAGC TGAGTTTTGG ATCATCGAAT 
CCTGCATCGG ATTATGACAG TCCAAAAGTA AAGCCGTCTG CCTCGGCTAA TGCCATCTAT 
TCCTTGGCAA TACGGCCTGT TGCAGCAGCA AGAACACAGA CAGGGGAGGA AACCAATGAG 
AATGACGAGG AGTCCGAGTA CATGACTCCA TCTTCTCGAC CAGTGCTGCC CTCTGCTGCA 
GGGGAGGCTG TGCCCAGACC ACCTCCACCA CTCTCACTCA GTCCCAGTAT GCTCAATGAA 
TTAGAGTCAG AAAGTCCACA GATGTATGAA GCCATGTACA ACATCCAAGC ACAGGCCCTC 
ACAGCTTCTG CACCACAGGC CAGAGACTCG GATTACTCCG AATTGCCTCC TTTGACCTGC 
TCTAATGGCC CGCGGGATCC TGTCGGTGCA TATGCTGAGG AGGAGGAGGA GGAGGAGAAC 
AGCTATGACT ATCCCAAACC TCATCTATCT GCAGCTCTAG CCCGACGCAC GCTTTCAGAT 
GCTAACTCAC CATCCTCCTC AGCCTTCAGC CGCCTCTCCT TTGACAGTGA CCTTGGAGCA 
GCTGCAACTG TCCTGGACCC CTCTGAAGCC CCCGAGCGGC CTCCTAAGCC ATTGCCCCGT 
CGCATCAACT CGGATCGCAG ACCCAGTCCA GTTCCCCCCG GGGCGAGTCC AGGCCCCAGC 
CAGCAGATCA GCAGTGAGAT CGAGCACCTC TTGAGCCAGG GATACTCCCA TCAGGACATC 
CAAAAAGCCC TAATGATTGC ACAGAACAAT ATTGAAATGG CCAAGAACAT CCTGAGGGAG 
TTTGTGTCCA TCCCTTCTAC TGCACATATC TTAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157636.1
CDS	530..3268
Translation	MAGNLKKGGGLIGMMKDAFQPHHHLHHSHHQPGAVDKKTVEKCWKFMDKVVRLCQNPKLALKNSPPYILDLLPDTYQHLRTILSRYEGKMETLGDNEYFRVFMENLTKKTKQTISLFKDGKERMFEENSQPRRNLTKLSLIFSHMLAELKAIFPNGLFQGDNFRITKADAAEFWRRSFGDKTIVPWKVFRQALHEFHPISSGLEAMALKSTIDLTCNDYISVFEFDIFTRLFQPWSSLLRNWNSLAVTHPGYMAFLTYDEVKARLQKFIHKPGSYIFRLSCTRLGQWAIGYVTADGNILQTIPHNKPLFQALIDGFREGFYLFPDGRTQNPDLTGLCEPSPQDHIKVTQEQYELYCEMGSTFQLCKICAENDKDVKIEPCGHLMCTSCLTAWQESEGQGCPFCRCEIKGTEPIVVDPFDPKDPNSAASYGCRGMFGAEGAPSPSYDDDDDDRLEETPFMISKLVGSKVERPPSPMSISPQPVVPPVPPRLDLLPLRPTNPPGASSPGATSKAPSHHKDKPLPLPPALRDLPPPPPPDRPHPAGADNRLPRRPLPSTPGDPSRDKLPPAPPNRNMPDWNSRPVPKAPSQPPASGDTRGSRELSNRHSLPLQLPSTLDSRTEAPRNNGPPSLDHQLSFGSSNPASDYDSPKVKPSASANAIYSLAIRPVAAARTQTGEETNENDEESEYMTPSSRPVLPSAAGEAVPRPPPPLSLSPSMLNELESESPQMYEAMYNIQAQALTASAPQARDSDYSELPPLTCSNGPRDPVGAYAEEEEEEENSYDYPKPHLSAALARRTLSDANSPSSSAFSRLSFDSDLGAAATVLDPSEAPERPPKPLPRRINSDRRPSPVPPGASPGPSQQISSEIEHLLSQGYSHQDIQKALMIAQNNIEMAKNILREFVSIPSTAHILT

Target ORF information:

RefSeq Version	XM_005157579.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Cbl proto-oncogene, E3 ubiquitin protein ligase (cbl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157579.3

ORF Insert Sequence:

ATGGCAGGAA ATCTGAAGAA AGGTGGGGGG CTTATTGGCA TGATGAAGGA CGCTTTTCAG 
CCTCACCATC ACCTCCACCA CTCCCATCAC CAGCCCGGAG CCGTGGACAA AAAGACGGTG 
GAGAAATGCT GGAAATTCAT GGATAAGGTG GTACGGTTAT GCCAGAACCC CAAATTGGCC 
TTGAAGAACA GTCCTCCGTA CATCCTAGAC TTGCTGCCAG ATACATACCA GCATCTCCGC 
ACTATCCTGT CTCGATATGA AGGCAAGATG GAAACGCTAG GCGACAACGA GTACTTCCGT 
GTTTTTATGG AGAACTTGAC CAAGAAGACC AAACAGACCA TAAGCCTGTT CAAAGATGGC 
AAAGAGCGAA TGTTCGAGGA GAACTCTCAG CCCAGACGGA ACTTAACAAA GTTGTCGCTG 
ATTTTCAGTC ACATGCTCGC TGAGCTCAAA GCCATCTTTC CTAATGGTCT GTTTCAAGGG 
GACAACTTCA GAATCACCAA AGCGGATGCC GCAGAGTTCT GGAGAAGATC GTTCGGGGAC 
AAGACCATTG TGCCTTGGAA GGTGTTCCGT CAGGCCCTGC ATGAGTTTCA CCCCATCAGC 
TCAGGCCTGG AGGCCATGGC ACTGAAATCC ACCATCGACC TCACATGCAA CGACTACATC 
TCCGTTTTTG AGTTTGACAT CTTCACCAGG CTATTCCAGC CATGGTCGTC ATTGTTGAGA 
AACTGGAACA GCCTGGCAGT GACTCATCCC GGGTACATGG CTTTTCTAAC CTATGATGAA 
GTCAAAGCTC GTCTACAAAA ATTCATCCAC AAGCCTGGCA GCTATATCTT CAGACTGAGC 
TGTACGAGGC TCGGACAGTG GGCTATCGGT TACGTCACGG CTGATGGAAA CATCTTGCAG 
ACCATTCCTC ATAACAAACC CCTCTTCCAG GCCCTCATCG ATGGCTTTAG AGAAGGATTC 
TACCTTTTTC CAGATGGACG CACTCAAAAC CCAGACCTCA CAGGACTGTG TGAACCCTCA 
CCTCAAGACC ATATCAAAGT CACACAGGAG CAATATGAGC TGTACTGTGA GATGGGCTCT 
ACCTTCCAGC TGTGTAAGAT TTGTGCTGAG AATGACAAGG ATGTGAAGAT CGAGCCCTGC 
GGACACCTCA TGTGCACTTC CTGTCTTACA GCCTGGCAGG AGTCAGAAGG CCAAGGCTGC 
CCATTCTGTC GCTGTGAAAT TAAGGGTACA GAGCCCATCG TCGTTGACCC CTTTGATCCT 
AAGGATCCCA ACAGCGCTGC CTCCTATGGC TGCAGGGGCA TGTTTGGGGC TGAGGGAGCC 
CCTTCACCCA GCTACGACGA TGATGATGAT GACCGTTTAG AGGAAACTCC TTTCATGATT 
AGCAAGCTGG TCGGCTCTAA AGTGGAGAGG CCGCCCTCGC CGATGTCAAT CTCCCCTCAG 
CCCGTCGTTC CTCCAGTACC TCCTCGGCTG GATCTGCTTC CTCTCAGGCC CACCAACCCT 
CCTGGGGCCT CAAGTCCAGG GGCCACATCC AAGGCCCCTT CCCATCACAA AGACAAGCCA 
TTACCGCTGC CGCCGGCTCT AAGGGATCTG CCACCTCCAC CTCCCCCAGA CCGTCCACAC 
CCAGCTGGAG CAGACAACCG TCTCCCAAGA CGTCCTCTCC CCAGTACGCC TGGAGACCCA 
TCTCGTGACA AACTGCCCCC GGCCCCTCCT AACCGCAACA TGCCAGACTG GAACTCCCGG 
CCTGTACCCA AAGCCCCATC ACAGCCACCA GCAAGCGGAG ACACCCGTGG ATCTCGAGAG 
CTTTCCAACA GGCACTCTCT GCCTCTCCAA CTGCCCTCTA CTCTCGATTC CAGAACAGAA 
GCCCCCAGAA ACAACGGCCC TCCCAGCCTC GACCACCAGC TGAGTTTTGG ATCATCGAAT 
CCTGCATCGG ATTATGACAG TCCAAAAGTA AAGCCGTCTG CCTCGGCTAA TGCCATCTAT 
TCCTTGGCAA TACGGCCTGT TGCAGCAGCA AGAACACAGA CAGGGGAGGA AACCAATGAG 
AATGACGAGG AGTCCGAGTA CATGACTCCA TCTTCTCGAC CAGTGCTGCC CTCTGCTGCA 
GGGGAGGCTG TGCCCAGACC ACCTCCACCA CTCTCACTCA GTCCCAGTAT GCTCAATGAA 
TTAGAGTCAG AAAGTCCACA GATGTATGAA GCCATGTACA ACATCCAAGC ACAGGCCCTC 
ACAGCTTCTG CACCACAGGC CAGAGACTCG GATTACTCCG AATTGCCTCC TTTGACCTGC 
TCTAATGGCC CGCGGGATCC TGTCGGTGCA TATGCTGAGG AGGAGGAGGA GGAGGAGAAC 
AGCTATGACT ATCCCAAACC TCATCTATCT GCAGCTCTAG CCCGACGCAC GCTTTCAGAT 
GCTAACTCAC CATCCTCCTC AGCCTTCAGC CGCCTCTCCT TTGACAGTGA CCTTGGAGCA 
GCTGCAACTG TCCTGGACCC CTCTGAAGCC CCCGAGCGGC CTCCTAAGCC ATTGCCCCGT 
CGCATCAACT CGGATCGCAG ACCCAGTCCA GTTCCCCCCG GGGCGAGTCC AGGCCCCAGC 
CAGCAGATCA GCAGTGAGAT CGAGCACCTC TTGAGCCAGG GATACTCCCA TCAGGACATC 
CAAAAAGCCC TAATGATTGC ACAGAACAAT ATTGAAATGG CCAAGAACAT CCTGAGGGAG 
TTTGTGTCCA TCCCTTCTAC TGCACATATC TTAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cbl ( XM_005157579.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157636.1 Danio rerio Cbl proto-oncogene, E3 ubiquitin protein ligase (cbl), transcript variant X1, mRNA.

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CloneID	ODa20476
Clone ID Related Accession (Same CDS sequence)	XM_005157579.3 , XM_005157579.4
Accession Version	XM_005157579.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase CBL isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005157579.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGAA ATCTGAAGAA AGGTGGGGGG CTTATTGGCA TGATGAAGGA CGCTTTTCAG 
CCTCACCATC ACCTCCACCA CTCCCATCAC CAGCCCGGAG CCGTGGACAA AAAGACGGTG 
GAGAAATGCT GGAAATTCAT GGATAAGGTG GTACGGTTAT GCCAGAACCC CAAATTGGCC 
TTGAAGAACA GTCCTCCGTA CATCCTAGAC TTGCTGCCAG ATACATACCA GCATCTCCGC 
ACTATCCTGT CTCGATATGA AGGCAAGATG GAAACGCTAG GCGACAACGA GTACTTCCGT 
GTTTTTATGG AGAACTTGAC CAAGAAGACC AAACAGACCA TAAGCCTGTT CAAAGATGGC 
AAAGAGCGAA TGTTCGAGGA GAACTCTCAG CCCAGACGGA ACTTAACAAA GTTGTCGCTG 
ATTTTCAGTC ACATGCTCGC TGAGCTCAAA GCCATCTTTC CTAATGGTCT GTTTCAAGGG 
GACAACTTCA GAATCACCAA AGCGGATGCC GCAGAGTTCT GGAGAAGATC GTTCGGGGAC 
AAGACCATTG TGCCTTGGAA GGTGTTCCGT CAGGCCCTGC ATGAGTTTCA CCCCATCAGC 
TCAGGCCTGG AGGCCATGGC ACTGAAATCC ACCATCGACC TCACATGCAA CGACTACATC 
TCCGTTTTTG AGTTTGACAT CTTCACCAGG CTATTCCAGC CATGGTCGTC ATTGTTGAGA 
AACTGGAACA GCCTGGCAGT GACTCATCCC GGGTACATGG CTTTTCTAAC CTATGATGAA 
GTCAAAGCTC GTCTACAAAA ATTCATCCAC AAGCCTGGCA GCTATATCTT CAGACTGAGC 
TGTACGAGGC TCGGACAGTG GGCTATCGGT TACGTCACGG CTGATGGAAA CATCTTGCAG 
ACCATTCCTC ATAACAAACC CCTCTTCCAG GCCCTCATCG ATGGCTTTAG AGAAGGATTC 
TACCTTTTTC CAGATGGACG CACTCAAAAC CCAGACCTCA CAGGACTGTG TGAACCCTCA 
CCTCAAGACC ATATCAAAGT CACACAGGAG CAATATGAGC TGTACTGTGA GATGGGCTCT 
ACCTTCCAGC TGTGTAAGAT TTGTGCTGAG AATGACAAGG ATGTGAAGAT CGAGCCCTGC 
GGACACCTCA TGTGCACTTC CTGTCTTACA GCCTGGCAGG AGTCAGAAGG CCAAGGCTGC 
CCATTCTGTC GCTGTGAAAT TAAGGGTACA GAGCCCATCG TCGTTGACCC CTTTGATCCT 
AAGGATCCCA ACAGCGCTGC CTCCTATGGC TGCAGGGGCA TGTTTGGGGC TGAGGGAGCC 
CCTTCACCCA GCTACGACGA TGATGATGAT GACCGTTTAG AGGAAACTCC TTTCATGATT 
AGCAAGCTGG TCGGCTCTAA AGTGGAGAGG CCGCCCTCGC CGATGTCAAT CTCCCCTCAG 
CCCGTCGTTC CTCCAGTACC TCCTCGGCTG GATCTGCTTC CTCTCAGGCC CACCAACCCT 
CCTGGGGCCT CAAGTCCAGG GGCCACATCC AAGGCCCCTT CCCATCACAA AGACAAGCCA 
TTACCGCTGC CGCCGGCTCT AAGGGATCTG CCACCTCCAC CTCCCCCAGA CCGTCCACAC 
CCAGCTGGAG CAGACAACCG TCTCCCAAGA CGTCCTCTCC CCAGTACGCC TGGAGACCCA 
TCTCGTGACA AACTGCCCCC GGCCCCTCCT AACCGCAACA TGCCAGACTG GAACTCCCGG 
CCTGTACCCA AAGCCCCATC ACAGCCACCA GCAAGCGGAG ACACCCGTGG ATCTCGAGAG 
CTTTCCAACA GGCACTCTCT GCCTCTCCAA CTGCCCTCTA CTCTCGATTC CAGAACAGAA 
GCCCCCAGAA ACAACGGCCC TCCCAGCCTC GACCACCAGC TGAGTTTTGG ATCATCGAAT 
CCTGCATCGG ATTATGACAG TCCAAAAGTA AAGCCGTCTG CCTCGGCTAA TGCCATCTAT 
TCCTTGGCAA TACGGCCTGT TGCAGCAGCA AGAACACAGA CAGGGGAGGA AACCAATGAG 
AATGACGAGG AGTCCGAGTA CATGACTCCA TCTTCTCGAC CAGTGCTGCC CTCTGCTGCA 
GGGGAGGCTG TGCCCAGACC ACCTCCACCA CTCTCACTCA GTCCCAGTAT GCTCAATGAA 
TTAGAGTCAG AAAGTCCACA GATGTATGAA GCCATGTACA ACATCCAAGC ACAGGCCCTC 
ACAGCTTCTG CACCACAGGC CAGAGACTCG GATTACTCCG AATTGCCTCC TTTGACCTGC 
TCTAATGGCC CGCGGGATCC TGTCGGTGCA TATGCTGAGG AGGAGGAGGA GGAGGAGAAC 
AGCTATGACT ATCCCAAACC TCATCTATCT GCAGCTCTAG CCCGACGCAC GCTTTCAGAT 
GCTAACTCAC CATCCTCCTC AGCCTTCAGC CGCCTCTCCT TTGACAGTGA CCTTGGAGCA 
GCTGCAACTG TCCTGGACCC CTCTGAAGCC CCCGAGCGGC CTCCTAAGCC ATTGCCCCGT 
CGCATCAACT CGGATCGCAG ACCCAGTCCA GTTCCCCCCG GGGCGAGTCC AGGCCCCAGC 
CAGCAGATCA GCAGTGAGAT CGAGCACCTC TTGAGCCAGG GATACTCCCA TCAGGACATC 
CAAAAAGCCC TAATGATTGC ACAGAACAAT ATTGAAATGG CCAAGAACAT CCTGAGGGAG 
TTTGTGTCCA TCCCTTCTAC TGCACATATC TTAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157636.1
CDS	328..3066
Translation	MAGNLKKGGGLIGMMKDAFQPHHHLHHSHHQPGAVDKKTVEKCWKFMDKVVRLCQNPKLALKNSPPYILDLLPDTYQHLRTILSRYEGKMETLGDNEYFRVFMENLTKKTKQTISLFKDGKERMFEENSQPRRNLTKLSLIFSHMLAELKAIFPNGLFQGDNFRITKADAAEFWRRSFGDKTIVPWKVFRQALHEFHPISSGLEAMALKSTIDLTCNDYISVFEFDIFTRLFQPWSSLLRNWNSLAVTHPGYMAFLTYDEVKARLQKFIHKPGSYIFRLSCTRLGQWAIGYVTADGNILQTIPHNKPLFQALIDGFREGFYLFPDGRTQNPDLTGLCEPSPQDHIKVTQEQYELYCEMGSTFQLCKICAENDKDVKIEPCGHLMCTSCLTAWQESEGQGCPFCRCEIKGTEPIVVDPFDPKDPNSAASYGCRGMFGAEGAPSPSYDDDDDDRLEETPFMISKLVGSKVERPPSPMSISPQPVVPPVPPRLDLLPLRPTNPPGASSPGATSKAPSHHKDKPLPLPPALRDLPPPPPPDRPHPAGADNRLPRRPLPSTPGDPSRDKLPPAPPNRNMPDWNSRPVPKAPSQPPASGDTRGSRELSNRHSLPLQLPSTLDSRTEAPRNNGPPSLDHQLSFGSSNPASDYDSPKVKPSASANAIYSLAIRPVAAARTQTGEETNENDEESEYMTPSSRPVLPSAAGEAVPRPPPPLSLSPSMLNELESESPQMYEAMYNIQAQALTASAPQARDSDYSELPPLTCSNGPRDPVGAYAEEEEEEENSYDYPKPHLSAALARRTLSDANSPSSSAFSRLSFDSDLGAAATVLDPSEAPERPPKPLPRRINSDRRPSPVPPGASPGPSQQISSEIEHLLSQGYSHQDIQKALMIAQNNIEMAKNILREFVSIPSTAHILT

Target ORF information:

RefSeq Version	XM_005157579.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Cbl proto-oncogene, E3 ubiquitin protein ligase (cbl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157579.4

ORF Insert Sequence:

ATGGCAGGAA ATCTGAAGAA AGGTGGGGGG CTTATTGGCA TGATGAAGGA CGCTTTTCAG 
CCTCACCATC ACCTCCACCA CTCCCATCAC CAGCCCGGAG CCGTGGACAA AAAGACGGTG 
GAGAAATGCT GGAAATTCAT GGATAAGGTG GTACGGTTAT GCCAGAACCC CAAATTGGCC 
TTGAAGAACA GTCCTCCGTA CATCCTAGAC TTGCTGCCAG ATACATACCA GCATCTCCGC 
ACTATCCTGT CTCGATATGA AGGCAAGATG GAAACGCTAG GCGACAACGA GTACTTCCGT 
GTTTTTATGG AGAACTTGAC CAAGAAGACC AAACAGACCA TAAGCCTGTT CAAAGATGGC 
AAAGAGCGAA TGTTCGAGGA GAACTCTCAG CCCAGACGGA ACTTAACAAA GTTGTCGCTG 
ATTTTCAGTC ACATGCTCGC TGAGCTCAAA GCCATCTTTC CTAATGGTCT GTTTCAAGGG 
GACAACTTCA GAATCACCAA AGCGGATGCC GCAGAGTTCT GGAGAAGATC GTTCGGGGAC 
AAGACCATTG TGCCTTGGAA GGTGTTCCGT CAGGCCCTGC ATGAGTTTCA CCCCATCAGC 
TCAGGCCTGG AGGCCATGGC ACTGAAATCC ACCATCGACC TCACATGCAA CGACTACATC 
TCCGTTTTTG AGTTTGACAT CTTCACCAGG CTATTCCAGC CATGGTCGTC ATTGTTGAGA 
AACTGGAACA GCCTGGCAGT GACTCATCCC GGGTACATGG CTTTTCTAAC CTATGATGAA 
GTCAAAGCTC GTCTACAAAA ATTCATCCAC AAGCCTGGCA GCTATATCTT CAGACTGAGC 
TGTACGAGGC TCGGACAGTG GGCTATCGGT TACGTCACGG CTGATGGAAA CATCTTGCAG 
ACCATTCCTC ATAACAAACC CCTCTTCCAG GCCCTCATCG ATGGCTTTAG AGAAGGATTC 
TACCTTTTTC CAGATGGACG CACTCAAAAC CCAGACCTCA CAGGACTGTG TGAACCCTCA 
CCTCAAGACC ATATCAAAGT CACACAGGAG CAATATGAGC TGTACTGTGA GATGGGCTCT 
ACCTTCCAGC TGTGTAAGAT TTGTGCTGAG AATGACAAGG ATGTGAAGAT CGAGCCCTGC 
GGACACCTCA TGTGCACTTC CTGTCTTACA GCCTGGCAGG AGTCAGAAGG CCAAGGCTGC 
CCATTCTGTC GCTGTGAAAT TAAGGGTACA GAGCCCATCG TCGTTGACCC CTTTGATCCT 
AAGGATCCCA ACAGCGCTGC CTCCTATGGC TGCAGGGGCA TGTTTGGGGC TGAGGGAGCC 
CCTTCACCCA GCTACGACGA TGATGATGAT GACCGTTTAG AGGAAACTCC TTTCATGATT 
AGCAAGCTGG TCGGCTCTAA AGTGGAGAGG CCGCCCTCGC CGATGTCAAT CTCCCCTCAG 
CCCGTCGTTC CTCCAGTACC TCCTCGGCTG GATCTGCTTC CTCTCAGGCC CACCAACCCT 
CCTGGGGCCT CAAGTCCAGG GGCCACATCC AAGGCCCCTT CCCATCACAA AGACAAGCCA 
TTACCGCTGC CGCCGGCTCT AAGGGATCTG CCACCTCCAC CTCCCCCAGA CCGTCCACAC 
CCAGCTGGAG CAGACAACCG TCTCCCAAGA CGTCCTCTCC CCAGTACGCC TGGAGACCCA 
TCTCGTGACA AACTGCCCCC GGCCCCTCCT AACCGCAACA TGCCAGACTG GAACTCCCGG 
CCTGTACCCA AAGCCCCATC ACAGCCACCA GCAAGCGGAG ACACCCGTGG ATCTCGAGAG 
CTTTCCAACA GGCACTCTCT GCCTCTCCAA CTGCCCTCTA CTCTCGATTC CAGAACAGAA 
GCCCCCAGAA ACAACGGCCC TCCCAGCCTC GACCACCAGC TGAGTTTTGG ATCATCGAAT 
CCTGCATCGG ATTATGACAG TCCAAAAGTA AAGCCGTCTG CCTCGGCTAA TGCCATCTAT 
TCCTTGGCAA TACGGCCTGT TGCAGCAGCA AGAACACAGA CAGGGGAGGA AACCAATGAG 
AATGACGAGG AGTCCGAGTA CATGACTCCA TCTTCTCGAC CAGTGCTGCC CTCTGCTGCA 
GGGGAGGCTG TGCCCAGACC ACCTCCACCA CTCTCACTCA GTCCCAGTAT GCTCAATGAA 
TTAGAGTCAG AAAGTCCACA GATGTATGAA GCCATGTACA ACATCCAAGC ACAGGCCCTC 
ACAGCTTCTG CACCACAGGC CAGAGACTCG GATTACTCCG AATTGCCTCC TTTGACCTGC 
TCTAATGGCC CGCGGGATCC TGTCGGTGCA TATGCTGAGG AGGAGGAGGA GGAGGAGAAC 
AGCTATGACT ATCCCAAACC TCATCTATCT GCAGCTCTAG CCCGACGCAC GCTTTCAGAT 
GCTAACTCAC CATCCTCCTC AGCCTTCAGC CGCCTCTCCT TTGACAGTGA CCTTGGAGCA 
GCTGCAACTG TCCTGGACCC CTCTGAAGCC CCCGAGCGGC CTCCTAAGCC ATTGCCCCGT 
CGCATCAACT CGGATCGCAG ACCCAGTCCA GTTCCCCCCG GGGCGAGTCC AGGCCCCAGC 
CAGCAGATCA GCAGTGAGAT CGAGCACCTC TTGAGCCAGG GATACTCCCA TCAGGACATC 
CAAAAAGCCC TAATGATTGC ACAGAACAAT ATTGAAATGG CCAAGAACAT CCTGAGGGAG 
TTTGTGTCCA TCCCTTCTAC TGCACATATC TTAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

A point mutation of zebrafish c-cbl gene in the ring finger domain produces a phenotype mimicking human myeloproliferative disease.
Leukemia29(12)2355-65(2015 Dec)
Peng X,Dong M,Ma L,Jia XE,Mao J,Jin C,Chen Y,Gao L,Liu X,Ma K,Wang L,Du T,Jin Y,Huang Q,Li K,Zon LI,Liu T,Deng M,Zhou Y,Xi X,Zhou Y,Chen S

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The c-Cbl ubiquitin ligase regulates nuclear ?-catenin and angiogenesis by its tyrosine phosphorylation mediated through the Wnt signaling pathway.
The Journal of biological chemistry290(20)12537-46(2015 May)
Shivanna S,Harrold I,Shashar M,Meyer R,Kiang C,Francis J,Zhao Q,Feng H,Edelman ER,Rahimi N,Chitalia VC

Somatic mutagenesis with a Sleeping Beauty transposon system leads to solid tumor formation in zebrafish.
PloS one6(4)e18826(2011 Apr)
McGrail M,Hatler JM,Kuang X,Liao HK,Nannapaneni K,Watt KE,Uhl JD,Largaespada DA,Vollbrecht E,Scheetz TE,Dupuy AJ,Hostetter JM,Essner JJ

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cbl cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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