acacb cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	acacb
Entrez Gene ID	556236
Full Name	acetyl-CoA carboxylase beta
Synonyms	si:ch211-250o15.3
General protein information	Preferred Names acetyl-CoA carboxylase beta Names acetyl-CoA carboxylase 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009301375.2	XP_009299650.1	acetyl-CoA carboxylase 2 isoform X1
XM_021476433.1	XP_021332108.1	acetyl-CoA carboxylase 2 isoform X2
XM_009301376.2	XP_009299651.1	acetyl-CoA carboxylase 2 isoform X2
XM_009301377.2	XP_009299652.1	acetyl-CoA carboxylase 2 isoform X3
XM_021476434.1	XP_021332109.1	acetyl-CoA carboxylase 2 isoform X3
XM_021476432.1	XP_021332107.1	acetyl-CoA carboxylase 2 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Acacb	NP_598665.2
Xenopus tropicalis (tropical clawed frog)	acacb	NP_001131086.1
Homo sapiens (human)	ACACB	NP_001084.3
	ACACB	XP_002798817.1
Bos taurus (cattle)	ACACB	NP_001192262.1
Danio rerio (zebrafish)	acacb	XP_005165251.1
Canis lupus familiaris (dog)	ACACB	XP_005636385.1
Pan troglodytes (chimpanzee)	ACACB	XP_003313981.1
Rattus norvegicus (Norway rat)	Acacb	NP_446374.1
Gallus gallus (chicken)	ACACB	XP_428114.4

Gene Ontology

The following acacb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the acacb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa10314	XM_021476433.1 Latest version!	Danio rerio acetyl-CoA carboxylase beta (acacb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa10314	XM_009301376.2 Latest version!	Danio rerio acetyl-CoA carboxylase beta (acacb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa10315	XM_021476434.1 Latest version!	Danio rerio acetyl-CoA carboxylase beta (acacb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa10315	XM_009301377.2 Latest version!	Danio rerio acetyl-CoA carboxylase beta (acacb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa42328	XM_009301375.2 Latest version!	Danio rerio acetyl-CoA carboxylase beta (acacb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa42328	XM_021476432.1 Latest version!	Danio rerio acetyl-CoA carboxylase beta (acacb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

acacb ( XM_021476433.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332108.1 Danio rerio acetyl-CoA carboxylase beta (acacb), transcript variant X2, mRNA.

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CloneID	ODa10314
Clone ID Related Accession (Same CDS sequence)	XM_021476433.1 , XM_009301376.2
Accession Version	XM_021476433.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	acetyl-CoA carboxylase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCATCAC AAACACCCAT CTCAGATTGT GCAGGGGAAC ATTCTCCACC AATGGACCCC 
GGATTATCAA GCACTTCACC AAGAAACGAG GGAATTCCTC CATCAGCAAC TAGCGATCCC 
ACTGACCAAT CAAATATCAC AGTTTCCCAT ATTGAATCCC ACAGGCCCAG GTTTGGTTCT 
GGTGCTCAGG CTAAAGAAAA ACTGAAGTTT ATTTTGGGTG CTTCTGAAGA TAATTCCTCT 
GATGATGAGC CTCTGGTTAA GAACCAAACC ACCACACATC AGCCCAAAAA CACAGAGACA 
CTTAATGCTG AACCTACCGC ATCATCAGAA ATGCCGCCAA CACCAGTTCC ATCGACGGGT 
TTAAGGCCTA GCATGTCAGG CCCCCACCTG CTGAAAAAGG GCAAAGAACA CAGGAAGATG 
GAGGTGCATA GAGATTTCAC CGTTGCCTCT CCAGCGGAGT TTGTCACTCG CTTTGGAGGA 
AACCGCATTA TAGATAAGGT GCTGATTGCA AATAATGGCA TTGCTGCAGT TAAGTGCATG 
CGGTCAATTC GCCGCTGGTC CTATGAAATG TTTCGTAATG AGAGAACCAT TCGCTTTGTG 
GTGATGGTCA CACCCGAAGA CTTGAAAGCC AATGCAGAAT ACATTAAAAT GGCTGATCAC 
TATGTGCCAG TACCAGGGGG TCCAAATAAC AACAACTATG CCAATGTTGA GATGATTGTG 
GATATTGCAA AAAGGATACC AGTGCAGGCT GTGTGGGCTG GATGGGGACA TGCATCTGAG 
AACCCCAAAC TACCAGAGCT CCTTCATAAA TCAGGGATAT GTTTTCTAGG ACCGTCCAGT 
AAAGCCATGT GGGCATTAGG AGATAAAGTG GCATCTTCAA TCGTAGCTCA GAGCGCAGGC 
ATTCCCACGT TGCCCTGGAG TGGAACTGGT CTGAGTGTTG AATGGGCAGA AGAAGAGCAG 
AGGCAGGGTC GTTTGATCAG CGTTCCTCCT GAACTATACG TGCAGGGTTG TGTTAAAGAT 
GTGGACGAGG GACTAGCGAG TGCAGAAAAG ATTGGCTACC CTGTGGTCAT CAAAGCATCA 
GAGGGAGGTG GAGGTAAAGG CATAAGAAAA GTTGAAAGTT CTGAAGACTT TCCTAGCCTT 
TTCAGACAGG TGCAGGCTGA GGTGCCAGGT TCACCCATCT TCATAATGCA ACTAGCCGAA 
CACGCACGCC ACCTGGAGGT GCAGATCTTG GCCGACCAAT ATGGCAATGC CATCTCTCTG 
TTTGGCAGAG ACTGTTCTAT CCAGCGCCGT CACCAGAAGA TCATAGAAGA AGCTCCTGCC 
AGCATTGCCT CCACCATCAC TTTTGAGCAA ATGGAGCAGT ATGCAGTTCG TCTGGCTAAA 
ATGGTGGGCT ATGTGAGCGC TGGTACGGTG GAATACCTGT TTTCTGAGGA TGGAAGTTTC 
CACTTCCTGG AGCTGAATCC TCGACTTCAG GTGGAGCATC CTTGTACGGA GATGATTGCT 
GACGTCAACC TTCCTGCAGC ACAGCTACAG ATAGCGATGG GGATTCCTCT TTACAGAATT 
AAGGATATCC GTGTCCTGTT TGGTGAAGCT CCATGGGGAG ACACAACTAT TAACTTTGAA 
TCTCCAGAAT GCATGCCCTG TCCACGGGGA CACGTCATAG CTGCACGCAT CACCAGTGAA 
AACCCGGATG AGGGCTTTAA ACCAAGCTCA GGTACAGTTC AGGAGCTGAA CTTCCGTAGC 
AGTAAGAATG TATGGGGCTA CTTTAGTGTA GGTGCAACTG GAGGCCTACA TGAGTTTGCA 
GACTCTCAGT TTGGACACTG TTTCTCCTGG GGCGAGAACC GAGAAGAGGC CATCTCGAAC 
ATGGTGGTAG CCATGAAGGA GCTGTCAATC AGAGGAGACT TCAGGACAAC AGTGGAGTAC 
CTTATAAAAC TGCTGGAAAC AGAAAGTTTC AGAAACAATG ACATTGATAC TGGTTGGCTG 
GATCACCTTA TTGCTGATAA AGTGCAGGCA GAGAGGCCAG ATACTATGTT AGGTGTGGTA 
TGCGGAGCTC TACAAGTGGC TGATGCAAGT TTTAGAGAAA GCATGTCTGA CTTCCTGCAT 
TCACTGGAAA GGGGTCAGGT GTTGCCGGCT GCTAGTTTGG TCAACACAGT TAATGTTGAT 
CTGATCTATG ATGGCGTCAA ATACTGCCTG AAGGTAGCCC GTCAGTCACC TACGACATAT 
GTTATCATAA TGAATGACTC CGATATAGAA GTTGATGTCC ATAGGCTCAG TGATGGTGGT 
CTACTGCTTT CCTATGGCGG CAGCAGCTAC ACAACCTACA TGAAGGAGGA GATTGATAGG 
TACCGTGTCA CAGTGGGCAA TAAAACGTGC GTGTTTGAGA AGGAGCGAGA CCCTACGGTG 
CTCCGTTCGC CCTCTGCTGG CAAGCTTTTG CAGTATGTAG TCACTGATGG TTCACATGTT 
TCGGCTTCCC AGCCTTATGC TGAAATTGAG GTGATGAAGA TGGTGATGAC CCTGCATGTC 
CAGCATTCTG GTTGTATTCG CTTTTTAAAA AGACCAGGAA CAGTATTGGA GCCTGGCTGT 
ATAGTGGCCC TAATGGACCT GGATGACCCC AGCTGTATTC ATCAGGTGAA ACCAAACACA 
GAGCCATTAC CAGCTCAGGA GCCATTACCC ATGGTAGGAG AGAGGCTTCA CCAGGTGTTC 
CACAATGTTC TGGAGAACCT GGTGAAAATT ATGGATGGAT ATTGTCTGCC AGAGCCATAT 
TTTAGTCAGA AATTGAAGAA CTGGGTCGAC ACGCTGATGA AGACGCTTCG GGATCCGTCT 
CTGCCTCTAC TTGAACTGCA GGAGATCATG ACCAGCGTGG CAGGGCGAAT TCCAGTTACT 
GTGGAGAAGG CAATTCGAAA GGTCATGGCA CAGTACGCCA GTAACATTAC CTCTGTGCTC 
TGTCAGTTCC CCAGCCAAAG GATAGCAAAT ATACTTGATA GCCATGCTGC CACACTTCAA 
AGGAAAGCAG ACCGGGAAAT TTTCTTCATG AACACCCAGA GCATTGTGCA ATTGGTGCAA 
AGATACCGTA GCGGCATTAG GGGCTACATG AAATCTGTTG TTCTGGATCT TTTGAAACAC 
TACCTTCAGG TGGAGATGCA ATTTCAGCAA GCTCACTATG ACAAGTGTGT GATCAATCTC 
AGAGAGCAGT ATAAGCCGGA CATGACCCCA GTGCTGGAGT GCATCTTTTC TCATGCTCAG 
GTGTCCAAGA AGAACATCCT CGTCACCATG CTCATAGACC AGCTGTGTGG CCGGGACCCA 
ATGCTTGCTG ATGAGCTCAT GGTCATTCTG GATGAGCTTA CACAACTCAG TAAAATGGAA 
AACTCTAAAG TGGCACTTAG GGCCAGACAG GTGTTGATCG CCTCTCATTT GCCCTCATAT 
GAACTCAGGC ACAACCAGGT GGAATCCATC TTTTTATCAG CTATTGATAT GTATGGGCAT 
CAGTTTTGTC CTGAAAACCT CAAGAAACTA ATCCTATCTG AGACCTCCAT CTTTGACGTC 
TTGCCCAGTT TTTTCTACCA CAACAACCGT GTGGTTTGCA TGGCTGCATT AGAGGTTTAT 
GTACGGAGGG CTTATATAGC TTACGAGCTA AACAGTCTTC AGCACCATCA GCTGCAGGAT 
GGGACATGTG CGGTGGATTT CCAGTTCATG TTACCATCAT CCCACCCAAA CAGAGGGAGC 
AGTCCTACTT TGAACAGACT GACCCTTCCT GTCAACGATT CGGGAGAGTT TCCGGCAATG 
CGCCGTCAAG GCAGTGAGCT CTTTCTAGAG GGGGCGCTTT CACCCCCCTG CCAGAGGATG 
GGAGCCATGG TTGCCTTCCA TAGCTTTGAC CACTTCAAAA GGTGCTTTGA TGAAGTGATC 
TGTCGCTTTG TGGATCCGCT GTGTGAAAGT TCTCTGTTTT TTGATGGCTG TTCCACCTTC 
TGTGATGGGG AGAGCTGTAA AAACATGAAG GAAAACCCCA TTCACATCAT AAATGTGTCA 
ATTAAACAAG CAGACACAGA AGATGATGAT GCTTTGGTCA CAGCTTTCAC TGCCTTTGCT 
CACTCCAAAA AATCGCTACT CTATGATTAC GGAATCAGAA GGGTGACATT TTTAGTTGCA 
CAAAAGCGGG AGTTCCCAAA ATACTTCACG TTTAGGGCCA GAGATGAATT CCATGAAGAC 
AGAATCTACC GTAACCTGGA GCCTGCTCTG GCCTTCCAGC TGGAGCTGAA CCGCATGCGT 
AACTTTGACC TTAAGGCTGT TCCCTGTGCT CACCACCGCA TGCAGCTGTA CTTGGGTGCT 
GCTCGTGTGG AAGAGGGCGC AGAGGTCACT GATTATCGCT TCTTTATCAG AGCCATCATT 
CGCCACTCTG ACCTCATCAC CAAGGAGGCC TCCTTTGAGT ATTTGCAGAA TGAGGGTGAG 
AGGCTGTTGC TGGAGGCGAT GGATGAGCTG GAGGTGGCCT TTAGTAACAC ACCCACTCGG 
ACTGACTGCA ATCACATCTT CCTCAATTTT GTACCCACTG TTATTATGGA CCCATACAAG 
ATAGAGGAGT CTGTCCGCTC CATGGTGATG CGATACGGTA GTCGACTGTG GAAGCTGCGA 
GTTCTCCAGG CTGAGCTGAA AATCAACATC CGCCTCACCA CCACTGGAGA TGTTATTCCC 
ATACGCCTCT TTCTCACCAA TGAGTCTGGA TACTATCTGG ACATCAGCTT GTATAAAGAG 
GTCAATGACC CATCCAGTGG ACAGATAATG TTCCAGTCGT ATGGAGACAA ACAAGGCCCG 
CTGAACGGCA TGCTCATAAA CACACCATAC GTCACAAAGG ATTTACTCCA GGCCAAGCGC 
TTCCAGGCTC AGAGCCTCGG CACCACATAT GTCTATGACT TCCCTGAGAT GTTTAGACAG 
GCTTTGTTTA AGCTTTGGGG ACCAGGAGAC AGTTATCCTA AAGATGTGTT GATGTGTAAT 
GAGCTGGTCC TGGATTCTCA GGGGAACCTT GTGCAGATGA ACAGACTACC AGGAGACAAT 
GAGGTTGGAA TGGTAGCTTT CCGGATGAGG ATGAAGACTC CAGAGTACCC AGAGGGCCGA 
GATATCATTG TGATCTGTAA TGATATTACC CATATGATTG GCTCTTTTGG GCCTCAGGAG 
GATCAGCTTT TTATGCGAGC ATCTGAGCTG GCCAGAGCGG AGGGCATCCC GCGCATCTAC 
ATCTCAGCCA ACAGCGGAGC TCGAATCGGC CTGGCAGAGG AGATCCGCCA CATGTTTCAG 
GTGGCTTGGA TCGACCCTGA AGACCCATAT AAGGGTTTTA AGTACCTGTA TCTGACTCCT 
CAAGACTACA CCCGCATCAG TTCCTCCAAT TCTGTCCATT GTCACCATGT AGAGGAGGGA 
GGAGAGTCCA GATACATCCT CACAGACATC ATAGGAAAGG AAGAGGGTAT TGGAGTGGAG 
AATCTGAGGG GCTCGGGCAC AATAGCTGGA GAAACTTCTC AGGCTTATAA AGAAATAATC 
ACTATCAGCA TGGTCACGTG TCGGGCTATA GGAATCGGTG CCTATCTGGT ACGTCTAGGA 
CAAAGAGTCA TTCAAGTGGA AAACTCCCAT ATCATCCTGA CTGGAGCCGG GGCATTAAAC 
AAGGTGCTGG GTCGTGAAGT CTACACCTCC AATAACCAGC TTGGTGGGGT TCAGATCATG 
CACAACAATG GAGTGACACA CTCCACCGTG CCAGATGACT TTGAAGGAGT GCTCACTATT 
CTACAGTGGT TATCCTACAT GCCAAAGAGC AATCAAAGTC CAGTCCCAAT AAAGCCTGCC 
ACTGATCCAG TCGACAGAGA GATCGATTTT GTTCCCACAA AAGCCCCTTA TGACCCTCGC 
TGGCTGCTCT GTGGACGACC CCATCCCACT GTGAAGGGAG CATGGCAGAG TGGATTCTTT 
GATCATGGTT CATTTTTGGA GATAATGGCT ACATGGGCAC AGACAGTGGT GGTGGGCCGA 
GCCAGGCTTG GTGGGATTCC TCTTGGGGTC ATTGCTGTGG AGACCCGCAC AGTCGAGCTT 
GCCATTCCAG CAGATCCTGC CAACTTAGAC TCAGAGGCCA AGCTCCTCCA GCAAGCTGGT 
CAGGTGTGGT TTCCAGATTC TGCATATAAA ACGGCTCAGG CTATTGAGGA TTTCAATAGA 
GAGAAACTTC CCCTTATGGT GTTTGCCAAC TGGCGAGGAT TTTCTGGAGG CATGAAAGAT 
ATGTACGACC AGGTGCTCAA GTTTGGCTCT TATATCGTGG ATGCCCTGCG AGAATTCAGC 
CAGCCTGTGC TGGTTTACAT TCCTCCTAAT GCTGAGCTGA GAGGAGGATC ATGGGTCGTG 
ATCGACCCTA CTATAAACCT TCAGCACATG GAGCTCTATG CAGATCGAGA AAGCAGAGGG 
GGCGTTTTGG AGGCTGAGGG CACAGTGGAG ATCAAATTCA GAAAAAAAGA CCTGCTAAAG 
ACCATGCGAA GGATTGATCC AGTGTATTCT CGTCTGGCTG AGCAGCTAGG CAAACCAGAC 
TTACCGAGCC AGGAGCGTAA AGACCTGGAG GCAAAACTAA AGTCTAGAGA GGAGTTCCTT 
CTTCCCATCT ACCACCAGGT GGCAGTGCAG TTTGTTGACC TTCATGACAC TCCAGGACGG 
ATGCAGGAAA AGGGAGTCAT CACGGACATC CTGGACTGGA AGACTGCTAG GTCATTCTTT 
TACTGGCGTC TGAGGCGACT GCTGTTGGAG GAAGTGGTAA AGAAGGAGAT CATGCAGGCC 
AACCAAGACC TGAGCAACGG ACACATACAG TCCATGCTGC GCCGCTGGTT TGTGGAGACA 
GAAGGAGCTG TAAAAGCATA TCTGTGGGAC AATAACAAAG TGGTGGTGGA GTGGCTGGAA 
AACAACCTGT CCCAGCAGGA CGGAACACGT TCGGTCATCA GAGAAAACAT CAAATGCATA 
AAGAGGGACT ACGCTCTCAA ACACATCCGC AGCTTAGTCC AAGCCAATCC AGAAGTGACT 
ATGGACTGCA TCATTCACAT GGCACAGAAC ATTACGCCTT CACAGAGAGC CAAAGTCTGT 
CACCTGCTGG CCACCATGGA CAACTCCACC ACCAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332108.1
CDS	225..7343
Translation	MPSQTPISDCAGEHSPPMDPGLSSTSPRNEGIPPSATSDPTDQSNITVSHIESHRPRFGSGAQAKEKLKFILGASEDNSSDDEPLVKNQTTTHQPKNTETLNAEPTASSEMPPTPVPSTGLRPSMSGPHLLKKGKEHRKMEVHRDFTVASPAEFVTRFGGNRIIDKVLIANNGIAAVKCMRSIRRWSYEMFRNERTIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVEMIVDIAKRIPVQAVWAGWGHASENPKLPELLHKSGICFLGPSSKAMWALGDKVASSIVAQSAGIPTLPWSGTGLSVEWAEEEQRQGRLISVPPELYVQGCVKDVDEGLASAEKIGYPVVIKASEGGGGKGIRKVESSEDFPSLFRQVQAEVPGSPIFIMQLAEHARHLEVQILADQYGNAISLFGRDCSIQRRHQKIIEEAPASIASTITFEQMEQYAVRLAKMVGYVSAGTVEYLFSEDGSFHFLELNPRLQVEHPCTEMIADVNLPAAQLQIAMGIPLYRIKDIRVLFGEAPWGDTTINFESPECMPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSSKNVWGYFSVGATGGLHEFADSQFGHCFSWGENREEAISNMVVAMKELSIRGDFRTTVEYLIKLLETESFRNNDIDTGWLDHLIADKVQAERPDTMLGVVCGALQVADASFRESMSDFLHSLERGQVLPAASLVNTVNVDLIYDGVKYCLKVARQSPTTYVIIMNDSDIEVDVHRLSDGGLLLSYGGSSYTTYMKEEIDRYRVTVGNKTCVFEKERDPTVLRSPSAGKLLQYVVTDGSHVSASQPYAEIEVMKMVMTLHVQHSGCIRFLKRPGTVLEPGCIVALMDLDDPSCIHQVKPNTEPLPAQEPLPMVGERLHQVFHNVLENLVKIMDGYCLPEPYFSQKLKNWVDTLMKTLRDPSLPLLELQEIMTSVAGRIPVTVEKAIRKVMAQYASNITSVLCQFPSQRIANILDSHAATLQRKADREIFFMNTQSIVQLVQRYRSGIRGYMKSVVLDLLKHYLQVEMQFQQAHYDKCVINLREQYKPDMTPVLECIFSHAQVSKKNILVTMLIDQLCGRDPMLADELMVILDELTQLSKMENSKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCPENLKKLILSETSIFDVLPSFFYHNNRVVCMAALEVYVRRAYIAYELNSLQHHQLQDGTCAVDFQFMLPSSHPNRGSSPTLNRLTLPVNDSGEFPAMRRQGSELFLEGALSPPCQRMGAMVAFHSFDHFKRCFDEVICRFVDPLCESSLFFDGCSTFCDGESCKNMKENPIHIINVSIKQADTEDDDALVTAFTAFAHSKKSLLYDYGIRRVTFLVAQKREFPKYFTFRARDEFHEDRIYRNLEPALAFQLELNRMRNFDLKAVPCAHHRMQLYLGAARVEEGAEVTDYRFFIRAIIRHSDLITKEASFEYLQNEGERLLLEAMDELEVAFSNTPTRTDCNHIFLNFVPTVIMDPYKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTTTGDVIPIRLFLTNESGYYLDISLYKEVNDPSSGQIMFQSYGDKQGPLNGMLINTPYVTKDLLQAKRFQAQSLGTTYVYDFPEMFRQALFKLWGPGDSYPKDVLMCNELVLDSQGNLVQMNRLPGDNEVGMVAFRMRMKTPEYPEGRDIIVICNDITHMIGSFGPQEDQLFMRASELARAEGIPRIYISANSGARIGLAEEIRHMFQVAWIDPEDPYKGFKYLYLTPQDYTRISSSNSVHCHHVEEGGESRYILTDIIGKEEGIGVENLRGSGTIAGETSQAYKEIITISMVTCRAIGIGAYLVRLGQRVIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHSTVPDDFEGVLTILQWLSYMPKSNQSPVPIKPATDPVDREIDFVPTKAPYDPRWLLCGRPHPTVKGAWQSGFFDHGSFLEIMATWAQTVVVGRARLGGIPLGVIAVETRTVELAIPADPANLDSEAKLLQQAGQVWFPDSAYKTAQAIEDFNREKLPLMVFANWRGFSGGMKDMYDQVLKFGSYIVDALREFSQPVLVYIPPNAELRGGSWVVIDPTINLQHMELYADRESRGGVLEAEGTVEIKFRKKDLLKTMRRIDPVYSRLAEQLGKPDLPSQERKDLEAKLKSREEFLLPIYHQVAVQFVDLHDTPGRMQEKGVITDILDWKTARSFFYWRLRRLLLEEVVKKEIMQANQDLSNGHIQSMLRRWFVETEGAVKAYLWDNNKVVVEWLENNLSQQDGTRSVIRENIKCIKRDYALKHIRSLVQANPEVTMDCIIHMAQNITPSQRAKVCHLLATMDNSTTS

Target ORF information:

RefSeq Version	XM_021476433.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase beta (acacb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476433.1

ORF Insert Sequence:

ATGCCATCAC AAACACCCAT CTCAGATTGT GCAGGGGAAC ATTCTCCACC AATGGACCCC 
GGATTATCAA GCACTTCACC AAGAAACGAG GGAATTCCTC CATCAGCAAC TAGCGATCCC 
ACTGACCAAT CAAATATCAC AGTTTCCCAT ATTGAATCCC ACAGGCCCAG GTTTGGTTCT 
GGTGCTCAGG CTAAAGAAAA ACTGAAGTTT ATTTTGGGTG CTTCTGAAGA TAATTCCTCT 
GATGATGAGC CTCTGGTTAA GAACCAAACC ACCACACATC AGCCCAAAAA CACAGAGACA 
CTTAATGCTG AACCTACCGC ATCATCAGAA ATGCCGCCAA CACCAGTTCC ATCGACGGGT 
TTAAGGCCTA GCATGTCAGG CCCCCACCTG CTGAAAAAGG GCAAAGAACA CAGGAAGATG 
GAGGTGCATA GAGATTTCAC CGTTGCCTCT CCAGCGGAGT TTGTCACTCG CTTTGGAGGA 
AACCGCATTA TAGATAAGGT GCTGATTGCA AATAATGGCA TTGCTGCAGT TAAGTGCATG 
CGGTCAATTC GCCGCTGGTC CTATGAAATG TTTCGTAATG AGAGAACCAT TCGCTTTGTG 
GTGATGGTCA CACCCGAAGA CTTGAAAGCC AATGCAGAAT ACATTAAAAT GGCTGATCAC 
TATGTGCCAG TACCAGGGGG TCCAAATAAC AACAACTATG CCAATGTTGA GATGATTGTG 
GATATTGCAA AAAGGATACC AGTGCAGGCT GTGTGGGCTG GATGGGGACA TGCATCTGAG 
AACCCCAAAC TACCAGAGCT CCTTCATAAA TCAGGGATAT GTTTTCTAGG ACCGTCCAGT 
AAAGCCATGT GGGCATTAGG AGATAAAGTG GCATCTTCAA TCGTAGCTCA GAGCGCAGGC 
ATTCCCACGT TGCCCTGGAG TGGAACTGGT CTGAGTGTTG AATGGGCAGA AGAAGAGCAG 
AGGCAGGGTC GTTTGATCAG CGTTCCTCCT GAACTATACG TGCAGGGTTG TGTTAAAGAT 
GTGGACGAGG GACTAGCGAG TGCAGAAAAG ATTGGCTACC CTGTGGTCAT CAAAGCATCA 
GAGGGAGGTG GAGGTAAAGG CATAAGAAAA GTTGAAAGTT CTGAAGACTT TCCTAGCCTT 
TTCAGACAGG TGCAGGCTGA GGTGCCAGGT TCACCCATCT TCATAATGCA ACTAGCCGAA 
CACGCACGCC ACCTGGAGGT GCAGATCTTG GCCGACCAAT ATGGCAATGC CATCTCTCTG 
TTTGGCAGAG ACTGTTCTAT CCAGCGCCGT CACCAGAAGA TCATAGAAGA AGCTCCTGCC 
AGCATTGCCT CCACCATCAC TTTTGAGCAA ATGGAGCAGT ATGCAGTTCG TCTGGCTAAA 
ATGGTGGGCT ATGTGAGCGC TGGTACGGTG GAATACCTGT TTTCTGAGGA TGGAAGTTTC 
CACTTCCTGG AGCTGAATCC TCGACTTCAG GTGGAGCATC CTTGTACGGA GATGATTGCT 
GACGTCAACC TTCCTGCAGC ACAGCTACAG ATAGCGATGG GGATTCCTCT TTACAGAATT 
AAGGATATCC GTGTCCTGTT TGGTGAAGCT CCATGGGGAG ACACAACTAT TAACTTTGAA 
TCTCCAGAAT GCATGCCCTG TCCACGGGGA CACGTCATAG CTGCACGCAT CACCAGTGAA 
AACCCGGATG AGGGCTTTAA ACCAAGCTCA GGTACAGTTC AGGAGCTGAA CTTCCGTAGC 
AGTAAGAATG TATGGGGCTA CTTTAGTGTA GGTGCAACTG GAGGCCTACA TGAGTTTGCA 
GACTCTCAGT TTGGACACTG TTTCTCCTGG GGCGAGAACC GAGAAGAGGC CATCTCGAAC 
ATGGTGGTAG CCATGAAGGA GCTGTCAATC AGAGGAGACT TCAGGACAAC AGTGGAGTAC 
CTTATAAAAC TGCTGGAAAC AGAAAGTTTC AGAAACAATG ACATTGATAC TGGTTGGCTG 
GATCACCTTA TTGCTGATAA AGTGCAGGCA GAGAGGCCAG ATACTATGTT AGGTGTGGTA 
TGCGGAGCTC TACAAGTGGC TGATGCAAGT TTTAGAGAAA GCATGTCTGA CTTCCTGCAT 
TCACTGGAAA GGGGTCAGGT GTTGCCGGCT GCTAGTTTGG TCAACACAGT TAATGTTGAT 
CTGATCTATG ATGGCGTCAA ATACTGCCTG AAGGTAGCCC GTCAGTCACC TACGACATAT 
GTTATCATAA TGAATGACTC CGATATAGAA GTTGATGTCC ATAGGCTCAG TGATGGTGGT 
CTACTGCTTT CCTATGGCGG CAGCAGCTAC ACAACCTACA TGAAGGAGGA GATTGATAGG 
TACCGTGTCA CAGTGGGCAA TAAAACGTGC GTGTTTGAGA AGGAGCGAGA CCCTACGGTG 
CTCCGTTCGC CCTCTGCTGG CAAGCTTTTG CAGTATGTAG TCACTGATGG TTCACATGTT 
TCGGCTTCCC AGCCTTATGC TGAAATTGAG GTGATGAAGA TGGTGATGAC CCTGCATGTC 
CAGCATTCTG GTTGTATTCG CTTTTTAAAA AGACCAGGAA CAGTATTGGA GCCTGGCTGT 
ATAGTGGCCC TAATGGACCT GGATGACCCC AGCTGTATTC ATCAGGTGAA ACCAAACACA 
GAGCCATTAC CAGCTCAGGA GCCATTACCC ATGGTAGGAG AGAGGCTTCA CCAGGTGTTC 
CACAATGTTC TGGAGAACCT GGTGAAAATT ATGGATGGAT ATTGTCTGCC AGAGCCATAT 
TTTAGTCAGA AATTGAAGAA CTGGGTCGAC ACGCTGATGA AGACGCTTCG GGATCCGTCT 
CTGCCTCTAC TTGAACTGCA GGAGATCATG ACCAGCGTGG CAGGGCGAAT TCCAGTTACT 
GTGGAGAAGG CAATTCGAAA GGTCATGGCA CAGTACGCCA GTAACATTAC CTCTGTGCTC 
TGTCAGTTCC CCAGCCAAAG GATAGCAAAT ATACTTGATA GCCATGCTGC CACACTTCAA 
AGGAAAGCAG ACCGGGAAAT TTTCTTCATG AACACCCAGA GCATTGTGCA ATTGGTGCAA 
AGATACCGTA GCGGCATTAG GGGCTACATG AAATCTGTTG TTCTGGATCT TTTGAAACAC 
TACCTTCAGG TGGAGATGCA ATTTCAGCAA GCTCACTATG ACAAGTGTGT GATCAATCTC 
AGAGAGCAGT ATAAGCCGGA CATGACCCCA GTGCTGGAGT GCATCTTTTC TCATGCTCAG 
GTGTCCAAGA AGAACATCCT CGTCACCATG CTCATAGACC AGCTGTGTGG CCGGGACCCA 
ATGCTTGCTG ATGAGCTCAT GGTCATTCTG GATGAGCTTA CACAACTCAG TAAAATGGAA 
AACTCTAAAG TGGCACTTAG GGCCAGACAG GTGTTGATCG CCTCTCATTT GCCCTCATAT 
GAACTCAGGC ACAACCAGGT GGAATCCATC TTTTTATCAG CTATTGATAT GTATGGGCAT 
CAGTTTTGTC CTGAAAACCT CAAGAAACTA ATCCTATCTG AGACCTCCAT CTTTGACGTC 
TTGCCCAGTT TTTTCTACCA CAACAACCGT GTGGTTTGCA TGGCTGCATT AGAGGTTTAT 
GTACGGAGGG CTTATATAGC TTACGAGCTA AACAGTCTTC AGCACCATCA GCTGCAGGAT 
GGGACATGTG CGGTGGATTT CCAGTTCATG TTACCATCAT CCCACCCAAA CAGAGGGAGC 
AGTCCTACTT TGAACAGACT GACCCTTCCT GTCAACGATT CGGGAGAGTT TCCGGCAATG 
CGCCGTCAAG GCAGTGAGCT CTTTCTAGAG GGGGCGCTTT CACCCCCCTG CCAGAGGATG 
GGAGCCATGG TTGCCTTCCA TAGCTTTGAC CACTTCAAAA GGTGCTTTGA TGAAGTGATC 
TGTCGCTTTG TGGATCCGCT GTGTGAAAGT TCTCTGTTTT TTGATGGCTG TTCCACCTTC 
TGTGATGGGG AGAGCTGTAA AAACATGAAG GAAAACCCCA TTCACATCAT AAATGTGTCA 
ATTAAACAAG CAGACACAGA AGATGATGAT GCTTTGGTCA CAGCTTTCAC TGCCTTTGCT 
CACTCCAAAA AATCGCTACT CTATGATTAC GGAATCAGAA GGGTGACATT TTTAGTTGCA 
CAAAAGCGGG AGTTCCCAAA ATACTTCACG TTTAGGGCCA GAGATGAATT CCATGAAGAC 
AGAATCTACC GTAACCTGGA GCCTGCTCTG GCCTTCCAGC TGGAGCTGAA CCGCATGCGT 
AACTTTGACC TTAAGGCTGT TCCCTGTGCT CACCACCGCA TGCAGCTGTA CTTGGGTGCT 
GCTCGTGTGG AAGAGGGCGC AGAGGTCACT GATTATCGCT TCTTTATCAG AGCCATCATT 
CGCCACTCTG ACCTCATCAC CAAGGAGGCC TCCTTTGAGT ATTTGCAGAA TGAGGGTGAG 
AGGCTGTTGC TGGAGGCGAT GGATGAGCTG GAGGTGGCCT TTAGTAACAC ACCCACTCGG 
ACTGACTGCA ATCACATCTT CCTCAATTTT GTACCCACTG TTATTATGGA CCCATACAAG 
ATAGAGGAGT CTGTCCGCTC CATGGTGATG CGATACGGTA GTCGACTGTG GAAGCTGCGA 
GTTCTCCAGG CTGAGCTGAA AATCAACATC CGCCTCACCA CCACTGGAGA TGTTATTCCC 
ATACGCCTCT TTCTCACCAA TGAGTCTGGA TACTATCTGG ACATCAGCTT GTATAAAGAG 
GTCAATGACC CATCCAGTGG ACAGATAATG TTCCAGTCGT ATGGAGACAA ACAAGGCCCG 
CTGAACGGCA TGCTCATAAA CACACCATAC GTCACAAAGG ATTTACTCCA GGCCAAGCGC 
TTCCAGGCTC AGAGCCTCGG CACCACATAT GTCTATGACT TCCCTGAGAT GTTTAGACAG 
GCTTTGTTTA AGCTTTGGGG ACCAGGAGAC AGTTATCCTA AAGATGTGTT GATGTGTAAT 
GAGCTGGTCC TGGATTCTCA GGGGAACCTT GTGCAGATGA ACAGACTACC AGGAGACAAT 
GAGGTTGGAA TGGTAGCTTT CCGGATGAGG ATGAAGACTC CAGAGTACCC AGAGGGCCGA 
GATATCATTG TGATCTGTAA TGATATTACC CATATGATTG GCTCTTTTGG GCCTCAGGAG 
GATCAGCTTT TTATGCGAGC ATCTGAGCTG GCCAGAGCGG AGGGCATCCC GCGCATCTAC 
ATCTCAGCCA ACAGCGGAGC TCGAATCGGC CTGGCAGAGG AGATCCGCCA CATGTTTCAG 
GTGGCTTGGA TCGACCCTGA AGACCCATAT AAGGGTTTTA AGTACCTGTA TCTGACTCCT 
CAAGACTACA CCCGCATCAG TTCCTCCAAT TCTGTCCATT GTCACCATGT AGAGGAGGGA 
GGAGAGTCCA GATACATCCT CACAGACATC ATAGGAAAGG AAGAGGGTAT TGGAGTGGAG 
AATCTGAGGG GCTCGGGCAC AATAGCTGGA GAAACTTCTC AGGCTTATAA AGAAATAATC 
ACTATCAGCA TGGTCACGTG TCGGGCTATA GGAATCGGTG CCTATCTGGT ACGTCTAGGA 
CAAAGAGTCA TTCAAGTGGA AAACTCCCAT ATCATCCTGA CTGGAGCCGG GGCATTAAAC 
AAGGTGCTGG GTCGTGAAGT CTACACCTCC AATAACCAGC TTGGTGGGGT TCAGATCATG 
CACAACAATG GAGTGACACA CTCCACCGTG CCAGATGACT TTGAAGGAGT GCTCACTATT 
CTACAGTGGT TATCCTACAT GCCAAAGAGC AATCAAAGTC CAGTCCCAAT AAAGCCTGCC 
ACTGATCCAG TCGACAGAGA GATCGATTTT GTTCCCACAA AAGCCCCTTA TGACCCTCGC 
TGGCTGCTCT GTGGACGACC CCATCCCACT GTGAAGGGAG CATGGCAGAG TGGATTCTTT 
GATCATGGTT CATTTTTGGA GATAATGGCT ACATGGGCAC AGACAGTGGT GGTGGGCCGA 
GCCAGGCTTG GTGGGATTCC TCTTGGGGTC ATTGCTGTGG AGACCCGCAC AGTCGAGCTT 
GCCATTCCAG CAGATCCTGC CAACTTAGAC TCAGAGGCCA AGCTCCTCCA GCAAGCTGGT 
CAGGTGTGGT TTCCAGATTC TGCATATAAA ACGGCTCAGG CTATTGAGGA TTTCAATAGA 
GAGAAACTTC CCCTTATGGT GTTTGCCAAC TGGCGAGGAT TTTCTGGAGG CATGAAAGAT 
ATGTACGACC AGGTGCTCAA GTTTGGCTCT TATATCGTGG ATGCCCTGCG AGAATTCAGC 
CAGCCTGTGC TGGTTTACAT TCCTCCTAAT GCTGAGCTGA GAGGAGGATC ATGGGTCGTG 
ATCGACCCTA CTATAAACCT TCAGCACATG GAGCTCTATG CAGATCGAGA AAGCAGAGGG 
GGCGTTTTGG AGGCTGAGGG CACAGTGGAG ATCAAATTCA GAAAAAAAGA CCTGCTAAAG 
ACCATGCGAA GGATTGATCC AGTGTATTCT CGTCTGGCTG AGCAGCTAGG CAAACCAGAC 
TTACCGAGCC AGGAGCGTAA AGACCTGGAG GCAAAACTAA AGTCTAGAGA GGAGTTCCTT 
CTTCCCATCT ACCACCAGGT GGCAGTGCAG TTTGTTGACC TTCATGACAC TCCAGGACGG 
ATGCAGGAAA AGGGAGTCAT CACGGACATC CTGGACTGGA AGACTGCTAG GTCATTCTTT 
TACTGGCGTC TGAGGCGACT GCTGTTGGAG GAAGTGGTAA AGAAGGAGAT CATGCAGGCC 
AACCAAGACC TGAGCAACGG ACACATACAG TCCATGCTGC GCCGCTGGTT TGTGGAGACA 
GAAGGAGCTG TAAAAGCATA TCTGTGGGAC AATAACAAAG TGGTGGTGGA GTGGCTGGAA 
AACAACCTGT CCCAGCAGGA CGGAACACGT TCGGTCATCA GAGAAAACAT CAAATGCATA 
AAGAGGGACT ACGCTCTCAA ACACATCCGC AGCTTAGTCC AAGCCAATCC AGAAGTGACT 
ATGGACTGCA TCATTCACAT GGCACAGAAC ATTACGCCTT CACAGAGAGC CAAAGTCTGT 
CACCTGCTGG CCACCATGGA CAACTCCACC ACCAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acacb ( XM_009301376.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299651.1 Danio rerio acetyl-CoA carboxylase beta (acacb), transcript variant X2, mRNA.

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CloneID	ODa10314
Clone ID Related Accession (Same CDS sequence)	XM_021476433.1 , XM_009301376.2
Accession Version	XM_009301376.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	acetyl-CoA carboxylase 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301376.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCATCAC AAACACCCAT CTCAGATTGT GCAGGGGAAC ATTCTCCACC AATGGACCCC 
GGATTATCAA GCACTTCACC AAGAAACGAG GGAATTCCTC CATCAGCAAC TAGCGATCCC 
ACTGACCAAT CAAATATCAC AGTTTCCCAT ATTGAATCCC ACAGGCCCAG GTTTGGTTCT 
GGTGCTCAGG CTAAAGAAAA ACTGAAGTTT ATTTTGGGTG CTTCTGAAGA TAATTCCTCT 
GATGATGAGC CTCTGGTTAA GAACCAAACC ACCACACATC AGCCCAAAAA CACAGAGACA 
CTTAATGCTG AACCTACCGC ATCATCAGAA ATGCCGCCAA CACCAGTTCC ATCGACGGGT 
TTAAGGCCTA GCATGTCAGG CCCCCACCTG CTGAAAAAGG GCAAAGAACA CAGGAAGATG 
GAGGTGCATA GAGATTTCAC CGTTGCCTCT CCAGCGGAGT TTGTCACTCG CTTTGGAGGA 
AACCGCATTA TAGATAAGGT GCTGATTGCA AATAATGGCA TTGCTGCAGT TAAGTGCATG 
CGGTCAATTC GCCGCTGGTC CTATGAAATG TTTCGTAATG AGAGAACCAT TCGCTTTGTG 
GTGATGGTCA CACCCGAAGA CTTGAAAGCC AATGCAGAAT ACATTAAAAT GGCTGATCAC 
TATGTGCCAG TACCAGGGGG TCCAAATAAC AACAACTATG CCAATGTTGA GATGATTGTG 
GATATTGCAA AAAGGATACC AGTGCAGGCT GTGTGGGCTG GATGGGGACA TGCATCTGAG 
AACCCCAAAC TACCAGAGCT CCTTCATAAA TCAGGGATAT GTTTTCTAGG ACCGTCCAGT 
AAAGCCATGT GGGCATTAGG AGATAAAGTG GCATCTTCAA TCGTAGCTCA GAGCGCAGGC 
ATTCCCACGT TGCCCTGGAG TGGAACTGGT CTGAGTGTTG AATGGGCAGA AGAAGAGCAG 
AGGCAGGGTC GTTTGATCAG CGTTCCTCCT GAACTATACG TGCAGGGTTG TGTTAAAGAT 
GTGGACGAGG GACTAGCGAG TGCAGAAAAG ATTGGCTACC CTGTGGTCAT CAAAGCATCA 
GAGGGAGGTG GAGGTAAAGG CATAAGAAAA GTTGAAAGTT CTGAAGACTT TCCTAGCCTT 
TTCAGACAGG TGCAGGCTGA GGTGCCAGGT TCACCCATCT TCATAATGCA ACTAGCCGAA 
CACGCACGCC ACCTGGAGGT GCAGATCTTG GCCGACCAAT ATGGCAATGC CATCTCTCTG 
TTTGGCAGAG ACTGTTCTAT CCAGCGCCGT CACCAGAAGA TCATAGAAGA AGCTCCTGCC 
AGCATTGCCT CCACCATCAC TTTTGAGCAA ATGGAGCAGT ATGCAGTTCG TCTGGCTAAA 
ATGGTGGGCT ATGTGAGCGC TGGTACGGTG GAATACCTGT TTTCTGAGGA TGGAAGTTTC 
CACTTCCTGG AGCTGAATCC TCGACTTCAG GTGGAGCATC CTTGTACGGA GATGATTGCT 
GACGTCAACC TTCCTGCAGC ACAGCTACAG ATAGCGATGG GGATTCCTCT TTACAGAATT 
AAGGATATCC GTGTCCTGTT TGGTGAAGCT CCATGGGGAG ACACAACTAT TAACTTTGAA 
TCTCCAGAAT GCATGCCCTG TCCACGGGGA CACGTCATAG CTGCACGCAT CACCAGTGAA 
AACCCGGATG AGGGCTTTAA ACCAAGCTCA GGTACAGTTC AGGAGCTGAA CTTCCGTAGC 
AGTAAGAATG TATGGGGCTA CTTTAGTGTA GGTGCAACTG GAGGCCTACA TGAGTTTGCA 
GACTCTCAGT TTGGACACTG TTTCTCCTGG GGCGAGAACC GAGAAGAGGC CATCTCGAAC 
ATGGTGGTAG CCATGAAGGA GCTGTCAATC AGAGGAGACT TCAGGACAAC AGTGGAGTAC 
CTTATAAAAC TGCTGGAAAC AGAAAGTTTC AGAAACAATG ACATTGATAC TGGTTGGCTG 
GATCACCTTA TTGCTGATAA AGTGCAGGCA GAGAGGCCAG ATACTATGTT AGGTGTGGTA 
TGCGGAGCTC TACAAGTGGC TGATGCAAGT TTTAGAGAAA GCATGTCTGA CTTCCTGCAT 
TCACTGGAAA GGGGTCAGGT GTTGCCGGCT GCTAGTTTGG TCAACACAGT TAATGTTGAT 
CTGATCTATG ATGGCGTCAA ATACTGCCTG AAGGTAGCCC GTCAGTCACC TACGACATAT 
GTTATCATAA TGAATGACTC CGATATAGAA GTTGATGTCC ATAGGCTCAG TGATGGTGGT 
CTACTGCTTT CCTATGGCGG CAGCAGCTAC ACAACCTACA TGAAGGAGGA GATTGATAGG 
TACCGTGTCA CAGTGGGCAA TAAAACGTGC GTGTTTGAGA AGGAGCGAGA CCCTACGGTG 
CTCCGTTCGC CCTCTGCTGG CAAGCTTTTG CAGTATGTAG TCACTGATGG TTCACATGTT 
TCGGCTTCCC AGCCTTATGC TGAAATTGAG GTGATGAAGA TGGTGATGAC CCTGCATGTC 
CAGCATTCTG GTTGTATTCG CTTTTTAAAA AGACCAGGAA CAGTATTGGA GCCTGGCTGT 
ATAGTGGCCC TAATGGACCT GGATGACCCC AGCTGTATTC ATCAGGTGAA ACCAAACACA 
GAGCCATTAC CAGCTCAGGA GCCATTACCC ATGGTAGGAG AGAGGCTTCA CCAGGTGTTC 
CACAATGTTC TGGAGAACCT GGTGAAAATT ATGGATGGAT ATTGTCTGCC AGAGCCATAT 
TTTAGTCAGA AATTGAAGAA CTGGGTCGAC ACGCTGATGA AGACGCTTCG GGATCCGTCT 
CTGCCTCTAC TTGAACTGCA GGAGATCATG ACCAGCGTGG CAGGGCGAAT TCCAGTTACT 
GTGGAGAAGG CAATTCGAAA GGTCATGGCA CAGTACGCCA GTAACATTAC CTCTGTGCTC 
TGTCAGTTCC CCAGCCAAAG GATAGCAAAT ATACTTGATA GCCATGCTGC CACACTTCAA 
AGGAAAGCAG ACCGGGAAAT TTTCTTCATG AACACCCAGA GCATTGTGCA ATTGGTGCAA 
AGATACCGTA GCGGCATTAG GGGCTACATG AAATCTGTTG TTCTGGATCT TTTGAAACAC 
TACCTTCAGG TGGAGATGCA ATTTCAGCAA GCTCACTATG ACAAGTGTGT GATCAATCTC 
AGAGAGCAGT ATAAGCCGGA CATGACCCCA GTGCTGGAGT GCATCTTTTC TCATGCTCAG 
GTGTCCAAGA AGAACATCCT CGTCACCATG CTCATAGACC AGCTGTGTGG CCGGGACCCA 
ATGCTTGCTG ATGAGCTCAT GGTCATTCTG GATGAGCTTA CACAACTCAG TAAAATGGAA 
AACTCTAAAG TGGCACTTAG GGCCAGACAG GTGTTGATCG CCTCTCATTT GCCCTCATAT 
GAACTCAGGC ACAACCAGGT GGAATCCATC TTTTTATCAG CTATTGATAT GTATGGGCAT 
CAGTTTTGTC CTGAAAACCT CAAGAAACTA ATCCTATCTG AGACCTCCAT CTTTGACGTC 
TTGCCCAGTT TTTTCTACCA CAACAACCGT GTGGTTTGCA TGGCTGCATT AGAGGTTTAT 
GTACGGAGGG CTTATATAGC TTACGAGCTA AACAGTCTTC AGCACCATCA GCTGCAGGAT 
GGGACATGTG CGGTGGATTT CCAGTTCATG TTACCATCAT CCCACCCAAA CAGAGGGAGC 
AGTCCTACTT TGAACAGACT GACCCTTCCT GTCAACGATT CGGGAGAGTT TCCGGCAATG 
CGCCGTCAAG GCAGTGAGCT CTTTCTAGAG GGGGCGCTTT CACCCCCCTG CCAGAGGATG 
GGAGCCATGG TTGCCTTCCA TAGCTTTGAC CACTTCAAAA GGTGCTTTGA TGAAGTGATC 
TGTCGCTTTG TGGATCCGCT GTGTGAAAGT TCTCTGTTTT TTGATGGCTG TTCCACCTTC 
TGTGATGGGG AGAGCTGTAA AAACATGAAG GAAAACCCCA TTCACATCAT AAATGTGTCA 
ATTAAACAAG CAGACACAGA AGATGATGAT GCTTTGGTCA CAGCTTTCAC TGCCTTTGCT 
CACTCCAAAA AATCGCTACT CTATGATTAC GGAATCAGAA GGGTGACATT TTTAGTTGCA 
CAAAAGCGGG AGTTCCCAAA ATACTTCACG TTTAGGGCCA GAGATGAATT CCATGAAGAC 
AGAATCTACC GTAACCTGGA GCCTGCTCTG GCCTTCCAGC TGGAGCTGAA CCGCATGCGT 
AACTTTGACC TTAAGGCTGT TCCCTGTGCT CACCACCGCA TGCAGCTGTA CTTGGGTGCT 
GCTCGTGTGG AAGAGGGCGC AGAGGTCACT GATTATCGCT TCTTTATCAG AGCCATCATT 
CGCCACTCTG ACCTCATCAC CAAGGAGGCC TCCTTTGAGT ATTTGCAGAA TGAGGGTGAG 
AGGCTGTTGC TGGAGGCGAT GGATGAGCTG GAGGTGGCCT TTAGTAACAC ACCCACTCGG 
ACTGACTGCA ATCACATCTT CCTCAATTTT GTACCCACTG TTATTATGGA CCCATACAAG 
ATAGAGGAGT CTGTCCGCTC CATGGTGATG CGATACGGTA GTCGACTGTG GAAGCTGCGA 
GTTCTCCAGG CTGAGCTGAA AATCAACATC CGCCTCACCA CCACTGGAGA TGTTATTCCC 
ATACGCCTCT TTCTCACCAA TGAGTCTGGA TACTATCTGG ACATCAGCTT GTATAAAGAG 
GTCAATGACC CATCCAGTGG ACAGATAATG TTCCAGTCGT ATGGAGACAA ACAAGGCCCG 
CTGAACGGCA TGCTCATAAA CACACCATAC GTCACAAAGG ATTTACTCCA GGCCAAGCGC 
TTCCAGGCTC AGAGCCTCGG CACCACATAT GTCTATGACT TCCCTGAGAT GTTTAGACAG 
GCTTTGTTTA AGCTTTGGGG ACCAGGAGAC AGTTATCCTA AAGATGTGTT GATGTGTAAT 
GAGCTGGTCC TGGATTCTCA GGGGAACCTT GTGCAGATGA ACAGACTACC AGGAGACAAT 
GAGGTTGGAA TGGTAGCTTT CCGGATGAGG ATGAAGACTC CAGAGTACCC AGAGGGCCGA 
GATATCATTG TGATCTGTAA TGATATTACC CATATGATTG GCTCTTTTGG GCCTCAGGAG 
GATCAGCTTT TTATGCGAGC ATCTGAGCTG GCCAGAGCGG AGGGCATCCC GCGCATCTAC 
ATCTCAGCCA ACAGCGGAGC TCGAATCGGC CTGGCAGAGG AGATCCGCCA CATGTTTCAG 
GTGGCTTGGA TCGACCCTGA AGACCCATAT AAGGGTTTTA AGTACCTGTA TCTGACTCCT 
CAAGACTACA CCCGCATCAG TTCCTCCAAT TCTGTCCATT GTCACCATGT AGAGGAGGGA 
GGAGAGTCCA GATACATCCT CACAGACATC ATAGGAAAGG AAGAGGGTAT TGGAGTGGAG 
AATCTGAGGG GCTCGGGCAC AATAGCTGGA GAAACTTCTC AGGCTTATAA AGAAATAATC 
ACTATCAGCA TGGTCACGTG TCGGGCTATA GGAATCGGTG CCTATCTGGT ACGTCTAGGA 
CAAAGAGTCA TTCAAGTGGA AAACTCCCAT ATCATCCTGA CTGGAGCCGG GGCATTAAAC 
AAGGTGCTGG GTCGTGAAGT CTACACCTCC AATAACCAGC TTGGTGGGGT TCAGATCATG 
CACAACAATG GAGTGACACA CTCCACCGTG CCAGATGACT TTGAAGGAGT GCTCACTATT 
CTACAGTGGT TATCCTACAT GCCAAAGAGC AATCAAAGTC CAGTCCCAAT AAAGCCTGCC 
ACTGATCCAG TCGACAGAGA GATCGATTTT GTTCCCACAA AAGCCCCTTA TGACCCTCGC 
TGGCTGCTCT GTGGACGACC CCATCCCACT GTGAAGGGAG CATGGCAGAG TGGATTCTTT 
GATCATGGTT CATTTTTGGA GATAATGGCT ACATGGGCAC AGACAGTGGT GGTGGGCCGA 
GCCAGGCTTG GTGGGATTCC TCTTGGGGTC ATTGCTGTGG AGACCCGCAC AGTCGAGCTT 
GCCATTCCAG CAGATCCTGC CAACTTAGAC TCAGAGGCCA AGCTCCTCCA GCAAGCTGGT 
CAGGTGTGGT TTCCAGATTC TGCATATAAA ACGGCTCAGG CTATTGAGGA TTTCAATAGA 
GAGAAACTTC CCCTTATGGT GTTTGCCAAC TGGCGAGGAT TTTCTGGAGG CATGAAAGAT 
ATGTACGACC AGGTGCTCAA GTTTGGCTCT TATATCGTGG ATGCCCTGCG AGAATTCAGC 
CAGCCTGTGC TGGTTTACAT TCCTCCTAAT GCTGAGCTGA GAGGAGGATC ATGGGTCGTG 
ATCGACCCTA CTATAAACCT TCAGCACATG GAGCTCTATG CAGATCGAGA AAGCAGAGGG 
GGCGTTTTGG AGGCTGAGGG CACAGTGGAG ATCAAATTCA GAAAAAAAGA CCTGCTAAAG 
ACCATGCGAA GGATTGATCC AGTGTATTCT CGTCTGGCTG AGCAGCTAGG CAAACCAGAC 
TTACCGAGCC AGGAGCGTAA AGACCTGGAG GCAAAACTAA AGTCTAGAGA GGAGTTCCTT 
CTTCCCATCT ACCACCAGGT GGCAGTGCAG TTTGTTGACC TTCATGACAC TCCAGGACGG 
ATGCAGGAAA AGGGAGTCAT CACGGACATC CTGGACTGGA AGACTGCTAG GTCATTCTTT 
TACTGGCGTC TGAGGCGACT GCTGTTGGAG GAAGTGGTAA AGAAGGAGAT CATGCAGGCC 
AACCAAGACC TGAGCAACGG ACACATACAG TCCATGCTGC GCCGCTGGTT TGTGGAGACA 
GAAGGAGCTG TAAAAGCATA TCTGTGGGAC AATAACAAAG TGGTGGTGGA GTGGCTGGAA 
AACAACCTGT CCCAGCAGGA CGGAACACGT TCGGTCATCA GAGAAAACAT CAAATGCATA 
AAGAGGGACT ACGCTCTCAA ACACATCCGC AGCTTAGTCC AAGCCAATCC AGAAGTGACT 
ATGGACTGCA TCATTCACAT GGCACAGAAC ATTACGCCTT CACAGAGAGC CAAAGTCTGT 
CACCTGCTGG CCACCATGGA CAACTCCACC ACCAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299651.1
CDS	268..7386
Translation	MPSQTPISDCAGEHSPPMDPGLSSTSPRNEGIPPSATSDPTDQSNITVSHIESHRPRFGSGAQAKEKLKFILGASEDNSSDDEPLVKNQTTTHQPKNTETLNAEPTASSEMPPTPVPSTGLRPSMSGPHLLKKGKEHRKMEVHRDFTVASPAEFVTRFGGNRIIDKVLIANNGIAAVKCMRSIRRWSYEMFRNERTIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVEMIVDIAKRIPVQAVWAGWGHASENPKLPELLHKSGICFLGPSSKAMWALGDKVASSIVAQSAGIPTLPWSGTGLSVEWAEEEQRQGRLISVPPELYVQGCVKDVDEGLASAEKIGYPVVIKASEGGGGKGIRKVESSEDFPSLFRQVQAEVPGSPIFIMQLAEHARHLEVQILADQYGNAISLFGRDCSIQRRHQKIIEEAPASIASTITFEQMEQYAVRLAKMVGYVSAGTVEYLFSEDGSFHFLELNPRLQVEHPCTEMIADVNLPAAQLQIAMGIPLYRIKDIRVLFGEAPWGDTTINFESPECMPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSSKNVWGYFSVGATGGLHEFADSQFGHCFSWGENREEAISNMVVAMKELSIRGDFRTTVEYLIKLLETESFRNNDIDTGWLDHLIADKVQAERPDTMLGVVCGALQVADASFRESMSDFLHSLERGQVLPAASLVNTVNVDLIYDGVKYCLKVARQSPTTYVIIMNDSDIEVDVHRLSDGGLLLSYGGSSYTTYMKEEIDRYRVTVGNKTCVFEKERDPTVLRSPSAGKLLQYVVTDGSHVSASQPYAEIEVMKMVMTLHVQHSGCIRFLKRPGTVLEPGCIVALMDLDDPSCIHQVKPNTEPLPAQEPLPMVGERLHQVFHNVLENLVKIMDGYCLPEPYFSQKLKNWVDTLMKTLRDPSLPLLELQEIMTSVAGRIPVTVEKAIRKVMAQYASNITSVLCQFPSQRIANILDSHAATLQRKADREIFFMNTQSIVQLVQRYRSGIRGYMKSVVLDLLKHYLQVEMQFQQAHYDKCVINLREQYKPDMTPVLECIFSHAQVSKKNILVTMLIDQLCGRDPMLADELMVILDELTQLSKMENSKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCPENLKKLILSETSIFDVLPSFFYHNNRVVCMAALEVYVRRAYIAYELNSLQHHQLQDGTCAVDFQFMLPSSHPNRGSSPTLNRLTLPVNDSGEFPAMRRQGSELFLEGALSPPCQRMGAMVAFHSFDHFKRCFDEVICRFVDPLCESSLFFDGCSTFCDGESCKNMKENPIHIINVSIKQADTEDDDALVTAFTAFAHSKKSLLYDYGIRRVTFLVAQKREFPKYFTFRARDEFHEDRIYRNLEPALAFQLELNRMRNFDLKAVPCAHHRMQLYLGAARVEEGAEVTDYRFFIRAIIRHSDLITKEASFEYLQNEGERLLLEAMDELEVAFSNTPTRTDCNHIFLNFVPTVIMDPYKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTTTGDVIPIRLFLTNESGYYLDISLYKEVNDPSSGQIMFQSYGDKQGPLNGMLINTPYVTKDLLQAKRFQAQSLGTTYVYDFPEMFRQALFKLWGPGDSYPKDVLMCNELVLDSQGNLVQMNRLPGDNEVGMVAFRMRMKTPEYPEGRDIIVICNDITHMIGSFGPQEDQLFMRASELARAEGIPRIYISANSGARIGLAEEIRHMFQVAWIDPEDPYKGFKYLYLTPQDYTRISSSNSVHCHHVEEGGESRYILTDIIGKEEGIGVENLRGSGTIAGETSQAYKEIITISMVTCRAIGIGAYLVRLGQRVIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHSTVPDDFEGVLTILQWLSYMPKSNQSPVPIKPATDPVDREIDFVPTKAPYDPRWLLCGRPHPTVKGAWQSGFFDHGSFLEIMATWAQTVVVGRARLGGIPLGVIAVETRTVELAIPADPANLDSEAKLLQQAGQVWFPDSAYKTAQAIEDFNREKLPLMVFANWRGFSGGMKDMYDQVLKFGSYIVDALREFSQPVLVYIPPNAELRGGSWVVIDPTINLQHMELYADRESRGGVLEAEGTVEIKFRKKDLLKTMRRIDPVYSRLAEQLGKPDLPSQERKDLEAKLKSREEFLLPIYHQVAVQFVDLHDTPGRMQEKGVITDILDWKTARSFFYWRLRRLLLEEVVKKEIMQANQDLSNGHIQSMLRRWFVETEGAVKAYLWDNNKVVVEWLENNLSQQDGTRSVIRENIKCIKRDYALKHIRSLVQANPEVTMDCIIHMAQNITPSQRAKVCHLLATMDNSTTS

Target ORF information:

RefSeq Version	XM_009301376.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase beta (acacb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301376.2

ORF Insert Sequence:

ATGCCATCAC AAACACCCAT CTCAGATTGT GCAGGGGAAC ATTCTCCACC AATGGACCCC 
GGATTATCAA GCACTTCACC AAGAAACGAG GGAATTCCTC CATCAGCAAC TAGCGATCCC 
ACTGACCAAT CAAATATCAC AGTTTCCCAT ATTGAATCCC ACAGGCCCAG GTTTGGTTCT 
GGTGCTCAGG CTAAAGAAAA ACTGAAGTTT ATTTTGGGTG CTTCTGAAGA TAATTCCTCT 
GATGATGAGC CTCTGGTTAA GAACCAAACC ACCACACATC AGCCCAAAAA CACAGAGACA 
CTTAATGCTG AACCTACCGC ATCATCAGAA ATGCCGCCAA CACCAGTTCC ATCGACGGGT 
TTAAGGCCTA GCATGTCAGG CCCCCACCTG CTGAAAAAGG GCAAAGAACA CAGGAAGATG 
GAGGTGCATA GAGATTTCAC CGTTGCCTCT CCAGCGGAGT TTGTCACTCG CTTTGGAGGA 
AACCGCATTA TAGATAAGGT GCTGATTGCA AATAATGGCA TTGCTGCAGT TAAGTGCATG 
CGGTCAATTC GCCGCTGGTC CTATGAAATG TTTCGTAATG AGAGAACCAT TCGCTTTGTG 
GTGATGGTCA CACCCGAAGA CTTGAAAGCC AATGCAGAAT ACATTAAAAT GGCTGATCAC 
TATGTGCCAG TACCAGGGGG TCCAAATAAC AACAACTATG CCAATGTTGA GATGATTGTG 
GATATTGCAA AAAGGATACC AGTGCAGGCT GTGTGGGCTG GATGGGGACA TGCATCTGAG 
AACCCCAAAC TACCAGAGCT CCTTCATAAA TCAGGGATAT GTTTTCTAGG ACCGTCCAGT 
AAAGCCATGT GGGCATTAGG AGATAAAGTG GCATCTTCAA TCGTAGCTCA GAGCGCAGGC 
ATTCCCACGT TGCCCTGGAG TGGAACTGGT CTGAGTGTTG AATGGGCAGA AGAAGAGCAG 
AGGCAGGGTC GTTTGATCAG CGTTCCTCCT GAACTATACG TGCAGGGTTG TGTTAAAGAT 
GTGGACGAGG GACTAGCGAG TGCAGAAAAG ATTGGCTACC CTGTGGTCAT CAAAGCATCA 
GAGGGAGGTG GAGGTAAAGG CATAAGAAAA GTTGAAAGTT CTGAAGACTT TCCTAGCCTT 
TTCAGACAGG TGCAGGCTGA GGTGCCAGGT TCACCCATCT TCATAATGCA ACTAGCCGAA 
CACGCACGCC ACCTGGAGGT GCAGATCTTG GCCGACCAAT ATGGCAATGC CATCTCTCTG 
TTTGGCAGAG ACTGTTCTAT CCAGCGCCGT CACCAGAAGA TCATAGAAGA AGCTCCTGCC 
AGCATTGCCT CCACCATCAC TTTTGAGCAA ATGGAGCAGT ATGCAGTTCG TCTGGCTAAA 
ATGGTGGGCT ATGTGAGCGC TGGTACGGTG GAATACCTGT TTTCTGAGGA TGGAAGTTTC 
CACTTCCTGG AGCTGAATCC TCGACTTCAG GTGGAGCATC CTTGTACGGA GATGATTGCT 
GACGTCAACC TTCCTGCAGC ACAGCTACAG ATAGCGATGG GGATTCCTCT TTACAGAATT 
AAGGATATCC GTGTCCTGTT TGGTGAAGCT CCATGGGGAG ACACAACTAT TAACTTTGAA 
TCTCCAGAAT GCATGCCCTG TCCACGGGGA CACGTCATAG CTGCACGCAT CACCAGTGAA 
AACCCGGATG AGGGCTTTAA ACCAAGCTCA GGTACAGTTC AGGAGCTGAA CTTCCGTAGC 
AGTAAGAATG TATGGGGCTA CTTTAGTGTA GGTGCAACTG GAGGCCTACA TGAGTTTGCA 
GACTCTCAGT TTGGACACTG TTTCTCCTGG GGCGAGAACC GAGAAGAGGC CATCTCGAAC 
ATGGTGGTAG CCATGAAGGA GCTGTCAATC AGAGGAGACT TCAGGACAAC AGTGGAGTAC 
CTTATAAAAC TGCTGGAAAC AGAAAGTTTC AGAAACAATG ACATTGATAC TGGTTGGCTG 
GATCACCTTA TTGCTGATAA AGTGCAGGCA GAGAGGCCAG ATACTATGTT AGGTGTGGTA 
TGCGGAGCTC TACAAGTGGC TGATGCAAGT TTTAGAGAAA GCATGTCTGA CTTCCTGCAT 
TCACTGGAAA GGGGTCAGGT GTTGCCGGCT GCTAGTTTGG TCAACACAGT TAATGTTGAT 
CTGATCTATG ATGGCGTCAA ATACTGCCTG AAGGTAGCCC GTCAGTCACC TACGACATAT 
GTTATCATAA TGAATGACTC CGATATAGAA GTTGATGTCC ATAGGCTCAG TGATGGTGGT 
CTACTGCTTT CCTATGGCGG CAGCAGCTAC ACAACCTACA TGAAGGAGGA GATTGATAGG 
TACCGTGTCA CAGTGGGCAA TAAAACGTGC GTGTTTGAGA AGGAGCGAGA CCCTACGGTG 
CTCCGTTCGC CCTCTGCTGG CAAGCTTTTG CAGTATGTAG TCACTGATGG TTCACATGTT 
TCGGCTTCCC AGCCTTATGC TGAAATTGAG GTGATGAAGA TGGTGATGAC CCTGCATGTC 
CAGCATTCTG GTTGTATTCG CTTTTTAAAA AGACCAGGAA CAGTATTGGA GCCTGGCTGT 
ATAGTGGCCC TAATGGACCT GGATGACCCC AGCTGTATTC ATCAGGTGAA ACCAAACACA 
GAGCCATTAC CAGCTCAGGA GCCATTACCC ATGGTAGGAG AGAGGCTTCA CCAGGTGTTC 
CACAATGTTC TGGAGAACCT GGTGAAAATT ATGGATGGAT ATTGTCTGCC AGAGCCATAT 
TTTAGTCAGA AATTGAAGAA CTGGGTCGAC ACGCTGATGA AGACGCTTCG GGATCCGTCT 
CTGCCTCTAC TTGAACTGCA GGAGATCATG ACCAGCGTGG CAGGGCGAAT TCCAGTTACT 
GTGGAGAAGG CAATTCGAAA GGTCATGGCA CAGTACGCCA GTAACATTAC CTCTGTGCTC 
TGTCAGTTCC CCAGCCAAAG GATAGCAAAT ATACTTGATA GCCATGCTGC CACACTTCAA 
AGGAAAGCAG ACCGGGAAAT TTTCTTCATG AACACCCAGA GCATTGTGCA ATTGGTGCAA 
AGATACCGTA GCGGCATTAG GGGCTACATG AAATCTGTTG TTCTGGATCT TTTGAAACAC 
TACCTTCAGG TGGAGATGCA ATTTCAGCAA GCTCACTATG ACAAGTGTGT GATCAATCTC 
AGAGAGCAGT ATAAGCCGGA CATGACCCCA GTGCTGGAGT GCATCTTTTC TCATGCTCAG 
GTGTCCAAGA AGAACATCCT CGTCACCATG CTCATAGACC AGCTGTGTGG CCGGGACCCA 
ATGCTTGCTG ATGAGCTCAT GGTCATTCTG GATGAGCTTA CACAACTCAG TAAAATGGAA 
AACTCTAAAG TGGCACTTAG GGCCAGACAG GTGTTGATCG CCTCTCATTT GCCCTCATAT 
GAACTCAGGC ACAACCAGGT GGAATCCATC TTTTTATCAG CTATTGATAT GTATGGGCAT 
CAGTTTTGTC CTGAAAACCT CAAGAAACTA ATCCTATCTG AGACCTCCAT CTTTGACGTC 
TTGCCCAGTT TTTTCTACCA CAACAACCGT GTGGTTTGCA TGGCTGCATT AGAGGTTTAT 
GTACGGAGGG CTTATATAGC TTACGAGCTA AACAGTCTTC AGCACCATCA GCTGCAGGAT 
GGGACATGTG CGGTGGATTT CCAGTTCATG TTACCATCAT CCCACCCAAA CAGAGGGAGC 
AGTCCTACTT TGAACAGACT GACCCTTCCT GTCAACGATT CGGGAGAGTT TCCGGCAATG 
CGCCGTCAAG GCAGTGAGCT CTTTCTAGAG GGGGCGCTTT CACCCCCCTG CCAGAGGATG 
GGAGCCATGG TTGCCTTCCA TAGCTTTGAC CACTTCAAAA GGTGCTTTGA TGAAGTGATC 
TGTCGCTTTG TGGATCCGCT GTGTGAAAGT TCTCTGTTTT TTGATGGCTG TTCCACCTTC 
TGTGATGGGG AGAGCTGTAA AAACATGAAG GAAAACCCCA TTCACATCAT AAATGTGTCA 
ATTAAACAAG CAGACACAGA AGATGATGAT GCTTTGGTCA CAGCTTTCAC TGCCTTTGCT 
CACTCCAAAA AATCGCTACT CTATGATTAC GGAATCAGAA GGGTGACATT TTTAGTTGCA 
CAAAAGCGGG AGTTCCCAAA ATACTTCACG TTTAGGGCCA GAGATGAATT CCATGAAGAC 
AGAATCTACC GTAACCTGGA GCCTGCTCTG GCCTTCCAGC TGGAGCTGAA CCGCATGCGT 
AACTTTGACC TTAAGGCTGT TCCCTGTGCT CACCACCGCA TGCAGCTGTA CTTGGGTGCT 
GCTCGTGTGG AAGAGGGCGC AGAGGTCACT GATTATCGCT TCTTTATCAG AGCCATCATT 
CGCCACTCTG ACCTCATCAC CAAGGAGGCC TCCTTTGAGT ATTTGCAGAA TGAGGGTGAG 
AGGCTGTTGC TGGAGGCGAT GGATGAGCTG GAGGTGGCCT TTAGTAACAC ACCCACTCGG 
ACTGACTGCA ATCACATCTT CCTCAATTTT GTACCCACTG TTATTATGGA CCCATACAAG 
ATAGAGGAGT CTGTCCGCTC CATGGTGATG CGATACGGTA GTCGACTGTG GAAGCTGCGA 
GTTCTCCAGG CTGAGCTGAA AATCAACATC CGCCTCACCA CCACTGGAGA TGTTATTCCC 
ATACGCCTCT TTCTCACCAA TGAGTCTGGA TACTATCTGG ACATCAGCTT GTATAAAGAG 
GTCAATGACC CATCCAGTGG ACAGATAATG TTCCAGTCGT ATGGAGACAA ACAAGGCCCG 
CTGAACGGCA TGCTCATAAA CACACCATAC GTCACAAAGG ATTTACTCCA GGCCAAGCGC 
TTCCAGGCTC AGAGCCTCGG CACCACATAT GTCTATGACT TCCCTGAGAT GTTTAGACAG 
GCTTTGTTTA AGCTTTGGGG ACCAGGAGAC AGTTATCCTA AAGATGTGTT GATGTGTAAT 
GAGCTGGTCC TGGATTCTCA GGGGAACCTT GTGCAGATGA ACAGACTACC AGGAGACAAT 
GAGGTTGGAA TGGTAGCTTT CCGGATGAGG ATGAAGACTC CAGAGTACCC AGAGGGCCGA 
GATATCATTG TGATCTGTAA TGATATTACC CATATGATTG GCTCTTTTGG GCCTCAGGAG 
GATCAGCTTT TTATGCGAGC ATCTGAGCTG GCCAGAGCGG AGGGCATCCC GCGCATCTAC 
ATCTCAGCCA ACAGCGGAGC TCGAATCGGC CTGGCAGAGG AGATCCGCCA CATGTTTCAG 
GTGGCTTGGA TCGACCCTGA AGACCCATAT AAGGGTTTTA AGTACCTGTA TCTGACTCCT 
CAAGACTACA CCCGCATCAG TTCCTCCAAT TCTGTCCATT GTCACCATGT AGAGGAGGGA 
GGAGAGTCCA GATACATCCT CACAGACATC ATAGGAAAGG AAGAGGGTAT TGGAGTGGAG 
AATCTGAGGG GCTCGGGCAC AATAGCTGGA GAAACTTCTC AGGCTTATAA AGAAATAATC 
ACTATCAGCA TGGTCACGTG TCGGGCTATA GGAATCGGTG CCTATCTGGT ACGTCTAGGA 
CAAAGAGTCA TTCAAGTGGA AAACTCCCAT ATCATCCTGA CTGGAGCCGG GGCATTAAAC 
AAGGTGCTGG GTCGTGAAGT CTACACCTCC AATAACCAGC TTGGTGGGGT TCAGATCATG 
CACAACAATG GAGTGACACA CTCCACCGTG CCAGATGACT TTGAAGGAGT GCTCACTATT 
CTACAGTGGT TATCCTACAT GCCAAAGAGC AATCAAAGTC CAGTCCCAAT AAAGCCTGCC 
ACTGATCCAG TCGACAGAGA GATCGATTTT GTTCCCACAA AAGCCCCTTA TGACCCTCGC 
TGGCTGCTCT GTGGACGACC CCATCCCACT GTGAAGGGAG CATGGCAGAG TGGATTCTTT 
GATCATGGTT CATTTTTGGA GATAATGGCT ACATGGGCAC AGACAGTGGT GGTGGGCCGA 
GCCAGGCTTG GTGGGATTCC TCTTGGGGTC ATTGCTGTGG AGACCCGCAC AGTCGAGCTT 
GCCATTCCAG CAGATCCTGC CAACTTAGAC TCAGAGGCCA AGCTCCTCCA GCAAGCTGGT 
CAGGTGTGGT TTCCAGATTC TGCATATAAA ACGGCTCAGG CTATTGAGGA TTTCAATAGA 
GAGAAACTTC CCCTTATGGT GTTTGCCAAC TGGCGAGGAT TTTCTGGAGG CATGAAAGAT 
ATGTACGACC AGGTGCTCAA GTTTGGCTCT TATATCGTGG ATGCCCTGCG AGAATTCAGC 
CAGCCTGTGC TGGTTTACAT TCCTCCTAAT GCTGAGCTGA GAGGAGGATC ATGGGTCGTG 
ATCGACCCTA CTATAAACCT TCAGCACATG GAGCTCTATG CAGATCGAGA AAGCAGAGGG 
GGCGTTTTGG AGGCTGAGGG CACAGTGGAG ATCAAATTCA GAAAAAAAGA CCTGCTAAAG 
ACCATGCGAA GGATTGATCC AGTGTATTCT CGTCTGGCTG AGCAGCTAGG CAAACCAGAC 
TTACCGAGCC AGGAGCGTAA AGACCTGGAG GCAAAACTAA AGTCTAGAGA GGAGTTCCTT 
CTTCCCATCT ACCACCAGGT GGCAGTGCAG TTTGTTGACC TTCATGACAC TCCAGGACGG 
ATGCAGGAAA AGGGAGTCAT CACGGACATC CTGGACTGGA AGACTGCTAG GTCATTCTTT 
TACTGGCGTC TGAGGCGACT GCTGTTGGAG GAAGTGGTAA AGAAGGAGAT CATGCAGGCC 
AACCAAGACC TGAGCAACGG ACACATACAG TCCATGCTGC GCCGCTGGTT TGTGGAGACA 
GAAGGAGCTG TAAAAGCATA TCTGTGGGAC AATAACAAAG TGGTGGTGGA GTGGCTGGAA 
AACAACCTGT CCCAGCAGGA CGGAACACGT TCGGTCATCA GAGAAAACAT CAAATGCATA 
AAGAGGGACT ACGCTCTCAA ACACATCCGC AGCTTAGTCC AAGCCAATCC AGAAGTGACT 
ATGGACTGCA TCATTCACAT GGCACAGAAC ATTACGCCTT CACAGAGAGC CAAAGTCTGT 
CACCTGCTGG CCACCATGGA CAACTCCACC ACCAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acacb ( XM_009301377.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299652.1 Danio rerio acetyl-CoA carboxylase beta (acacb), transcript variant X3, mRNA.

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CloneID	ODa10315
Clone ID Related Accession (Same CDS sequence)	XM_009301377.2 , XM_021476434.1
Accession Version	XM_009301377.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	acetyl-CoA carboxylase 2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301377.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCATCAC AAACACCCAT CTCAGATTGT GCAGGGGAAC ATTCTCCACC AATGGACCCC 
GGATTATCAA GCACTTCACC AAGAAACGAG GGAATTCCTC CATCAGCAAC TAGCGATCCC 
ACTGACCAAT CAAATATCAC AGTTTCCCAT ATTGAATCCC ACAGGCCCAG GTTTGGTTCT 
GGTGCTCAGG CTAAAGAAAA ACTGAAGTTT ATTTTGGGTG CTTCTGAAGA TAATTCCTCT 
GATGATGAGC CTCTGGTTAA GAACCAAACC ACCACACATC AGCCCAAAAA CACAGAGACA 
CTTAATGCTG AACCTACCGC ATCATCAGAA ATGCCGCCAA CACCAGTTCC ATCGACGGGT 
TTAAGGCCTA GCATGTCAGG CCCCCACCTG CTGAAAAAGG GCAAAGAACA CAGGAAGATG 
GAGGTGCATA GAGATTTCAC CGTTGCCTCT CCAGCGGAGT TTGTCACTCG CTTTGGAGGA 
AACCGCATTA TAGATAAGGT GCTGATTGCA AATAATGGCA TTGCTGCAGT TAAGTGCATG 
CGGTCAATTC GCCGCTGGTC CTATGAAATG TTTCGTAATG AGAGAACCAT TCGCTTTGTG 
GTGATGGTCA CACCCGAAGA CTTGAAAGCC AATGCAGAAT ACATTAAAAT GGCTGATCAC 
TATGTGCCAG TACCAGGGGG TCCAAATAAC AACAACTATG CCAATGTTGA GATGATTGTG 
GATATTGCAA AAAGGATACC AGTGCAGGCT GTGTGGGCTG GATGGGGACA TGCATCTGAG 
AACCCCAAAC TACCAGAGCT CCTTCATAAA TCAGGGATAT GTTTTCTAGG ACCGTCCAGT 
AAAGCCATGT GGGCATTAGG AGATAAAGTG GCATCTTCAA TCGTAGCTCA GAGCGCAGGC 
ATTCCCACGT TGCCCTGGAG TGGAACTGGT CTGAGTGTTG AATGGGCAGA AGAAGAGCAG 
AGGCAGGGTC GTTTGATCAG CGTTCCTCCT GAACTATACG TGCAGGGTTG TGTTAAAGAT 
GTGGACGAGG GACTAGCGAG TGCAGAAAAG ATTGGCTACC CTGTGGTCAT CAAAGCATCA 
GAGGGAGGTG GAGGTAAAGG CATAAGAAAA GTTGAAAGTT CTGAAGACTT TCCTAGCCTT 
TTCAGACAGG TGCAGGCTGA GGTGCCAGGT TCACCCATCT TCATAATGCA ACTAGCCGAA 
CACGCACGCC ACCTGGAGGT GCAGATCTTG GCCGACCAAT ATGGCAATGC CATCTCTCTG 
TTTGGCAGAG ACTGTTCTAT CCAGCGCCGT CACCAGAAGA TCATAGAAGA AGCTCCTGCC 
AGCATTGCCT CCACCATCAC TTTTGAGCAA ATGGAGCAGT ATGCAGTTCG TCTGGCTAAA 
ATGGTGGGCT ATGTGAGCGC TGGTACGGTG GAATACCTGT TTTCTGAGGA TGGAAGTTTC 
CACTTCCTGG AGCTGAATCC TCGACTTCAG GTGGAGCATC CTTGTACGGA GATGATTGCT 
GACGTCAACC TTCCTGCAGC ACAGCTACAG ATAGCGATGG GGATTCCTCT TTACAGAATT 
AAGGATATCC GTGTCCTGTT TGGTGAAGCT CCATGGGGAG ACACAACTAT TAACTTTGAA 
TCTCCAGAAT GCATGCCCTG TCCACGGGGA CACGTCATAG CTGCACGCAT CACCAGTGAA 
AACCCGGATG AGGGCTTTAA ACCAAGCTCA GGTACAGTTC AGGAGCTGAA CTTCCGTAGC 
AGTAAGAATG TATGGGGCTA CTTTAGTGTA GGTGCAACTG GAGGCCTACA TGAGTTTGCA 
GACTCTCAGT TTGGACACTG TTTCTCCTGG GGCGAGAACC GAGAAGAGGC CATCTCGAAC 
ATGGTGGTAG CCATGAAGGA GCTGTCAATC AGAGGAGACT TCAGGACAAC AGTGGAGTAC 
CTTATAAAAC TGCTGGAAAC AGAAAGTTTC AGAAACAATG ACATTGATAC TGGTTGGCTG 
GATCACCTTA TTGCTGATAA AGTGCAGGCA GAGAGGCCAG ATACTATGTT AGGTGTGGTA 
TGCGGAGCTC TACAAGTGGC TGATGCAAGT TTTAGAGAAA GCATGTCTGA CTTCCTGCAT 
TCACTGGAAA GGGGTCAGGT GTTGCCGGCT GCTAGTTTGG TCAACACAGT TAATGTTGAT 
CTGATCTATG ATGGCGTCAA ATACTGCCTG AAGGTAGCCC GTCAGTCACC TACGACATAT 
GTTATCATAA TGAATGACTC CGATATAGAA GTTGATGTCC ATAGGCTCAG TGATGGTGGT 
CTACTGCTTT CCTATGGCGG CAGCAGCTAC ACAACCTACA TGAAGGAGGA GATTGATAGG 
TACCGTGTCA CAGTGGGCAA TAAAACGTGC GTGTTTGAGA AGGAGCGAGA CCCTACGGTG 
CTCCGTTCGC CCTCTGCTGG CAAGCTTTTG CAGTATGTAG TCACTGATGG TTCACATGTT 
TCGGCTTCCC AGCCTTATGC TGAAATTGAG GTGATGAAGA TGGTGATGAC CCTGCATGTC 
CAGCATTCTG GTTGTATTCG CTTTTTAAAA AGACCAGGAA CAGTATTGGA GCCTGGCTGT 
ATAGTGGCCC TAATGGACCT GGATGACCCC AGCTGTATTC ATCAGGTGAA ACCAAACACA 
GAGCCATTAC CAGCTCAGGA GCCATTACCC ATGGTAGGAG AGAGGCTTCA CCAGGTGTTC 
CACAATGTTC TGGAGAACCT GGTGAAAATT ATGGATGGAT ATTGTCTGCC AGAGCCATAT 
TTTAGTCAGA AATTGAAGAA CTGGGTCGAC ACGCTGATGA AGACGCTTCG GGATCCGTCT 
CTGCCTCTAC TTGAACTGCA GGAGATCATG ACCAGCGTGG CAGGGCGAAT TCCAGTTACT 
GTGGAGAAGG CAATTCGAAA GGTCATGGCA CAGTACGCCA GTAACATTAC CTCTGTGCTC 
TGTCAGTTCC CCAGCCAAAG GATAGCAAAT ATACTTGATA GCCATGCTGC CACACTTCAA 
AGGAAAGCAG ACCGGGAAAT TTTCTTCATG AACACCCAGA GCATTGTGCA ATTGGTGCAA 
AGATACCGTA GCGGCATTAG GGGCTACATG AAATCTGTTG TTCTGGATCT TTTGAAACAC 
TACCTTCAGG TGGAGATGCA ATTTCAGCAA GCTCACTATG ACAAGTGTGT GATCAATCTC 
AGAGAGCAGT ATAAGCCGGA CATGACCCCA GTGCTGGAGT GCATCTTTTC TCATGCTCAG 
GTGTCCAAGA AGAACATCCT CGTCACCATG CTCATAGACC AGCTGTGTGG CCGGGACCCA 
ATGCTTGCTG ATGAGCTCAT GGTCATTCTG GATGAGCTTA CACAACTCAG TAAAATGGAA 
AACTCTAAAG TGGCACTTAG GGCCAGACAG GTGTTGATCG CCTCTCATTT GCCCTCATAT 
GAACTCAGGC ACAACCAGGT GGAATCCATC TTTTTATCAG CTATTGATAT GTATGGGCAT 
CAGTTTTGTC CTGAAAACCT CAAGAAACTA ATCCTATCTG AGACCTCCAT CTTTGACGTC 
TTGCCCAGTT TTTTCTACCA CAACAACCGT GTGGTTTGCA TGGCTGCATT AGAGGTTTAT 
GTACGGAGGG CTTATATAGC TTACGAGCTA AACAGTCTTC AGCACCATCA GCTGCAGGAT 
GGGACATGTG CGGTGGATTT CCAGTTCATG TTACCATCAT CCCACCCAAA CAGACTGACC 
CTTCCTGTCA ACGATTCGGG AGAGTTTCCG GCAATGCGCC GTCAAGGCAG TGAGCTCTTT 
CTAGAGGGGG CGCTTTCACC CCCCTGCCAG AGGATGGGAG CCATGGTTGC CTTCCATAGC 
TTTGACCACT TCAAAAGGTG CTTTGATGAA GTGATCTGTC GCTTTGTGGA TCCGCTGTGT 
GAAAGTTCTC TGTTTTTTGA TGGCTGTTCC ACCTTCTGTG ATGGGGAGAG CTGTAAAAAC 
ATGAAGGAAA ACCCCATTCA CATCATAAAT GTGTCAATTA AACAAGCAGA CACAGAAGAT 
GATGATGCTT TGGTCACAGC TTTCACTGCC TTTGCTCACT CCAAAAAATC GCTACTCTAT 
GATTACGGAA TCAGAAGGGT GACATTTTTA GTTGCACAAA AGCGGGAGTT CCCAAAATAC 
TTCACGTTTA GGGCCAGAGA TGAATTCCAT GAAGACAGAA TCTACCGTAA CCTGGAGCCT 
GCTCTGGCCT TCCAGCTGGA GCTGAACCGC ATGCGTAACT TTGACCTTAA GGCTGTTCCC 
TGTGCTCACC ACCGCATGCA GCTGTACTTG GGTGCTGCTC GTGTGGAAGA GGGCGCAGAG 
GTCACTGATT ATCGCTTCTT TATCAGAGCC ATCATTCGCC ACTCTGACCT CATCACCAAG 
GAGGCCTCCT TTGAGTATTT GCAGAATGAG GGTGAGAGGC TGTTGCTGGA GGCGATGGAT 
GAGCTGGAGG TGGCCTTTAG TAACACACCC ACTCGGACTG ACTGCAATCA CATCTTCCTC 
AATTTTGTAC CCACTGTTAT TATGGACCCA TACAAGATAG AGGAGTCTGT CCGCTCCATG 
GTGATGCGAT ACGGTAGTCG ACTGTGGAAG CTGCGAGTTC TCCAGGCTGA GCTGAAAATC 
AACATCCGCC TCACCACCAC TGGAGATGTT ATTCCCATAC GCCTCTTTCT CACCAATGAG 
TCTGGATACT ATCTGGACAT CAGCTTGTAT AAAGAGGTCA ATGACCCATC CAGTGGACAG 
ATAATGTTCC AGTCGTATGG AGACAAACAA GGCCCGCTGA ACGGCATGCT CATAAACACA 
CCATACGTCA CAAAGGATTT ACTCCAGGCC AAGCGCTTCC AGGCTCAGAG CCTCGGCACC 
ACATATGTCT ATGACTTCCC TGAGATGTTT AGACAGGCTT TGTTTAAGCT TTGGGGACCA 
GGAGACAGTT ATCCTAAAGA TGTGTTGATG TGTAATGAGC TGGTCCTGGA TTCTCAGGGG 
AACCTTGTGC AGATGAACAG ACTACCAGGA GACAATGAGG TTGGAATGGT AGCTTTCCGG 
ATGAGGATGA AGACTCCAGA GTACCCAGAG GGCCGAGATA TCATTGTGAT CTGTAATGAT 
ATTACCCATA TGATTGGCTC TTTTGGGCCT CAGGAGGATC AGCTTTTTAT GCGAGCATCT 
GAGCTGGCCA GAGCGGAGGG CATCCCGCGC ATCTACATCT CAGCCAACAG CGGAGCTCGA 
ATCGGCCTGG CAGAGGAGAT CCGCCACATG TTTCAGGTGG CTTGGATCGA CCCTGAAGAC 
CCATATAAGG GTTTTAAGTA CCTGTATCTG ACTCCTCAAG ACTACACCCG CATCAGTTCC 
TCCAATTCTG TCCATTGTCA CCATGTAGAG GAGGGAGGAG AGTCCAGATA CATCCTCACA 
GACATCATAG GAAAGGAAGA GGGTATTGGA GTGGAGAATC TGAGGGGCTC GGGCACAATA 
GCTGGAGAAA CTTCTCAGGC TTATAAAGAA ATAATCACTA TCAGCATGGT CACGTGTCGG 
GCTATAGGAA TCGGTGCCTA TCTGGTACGT CTAGGACAAA GAGTCATTCA AGTGGAAAAC 
TCCCATATCA TCCTGACTGG AGCCGGGGCA TTAAACAAGG TGCTGGGTCG TGAAGTCTAC 
ACCTCCAATA ACCAGCTTGG TGGGGTTCAG ATCATGCACA ACAATGGAGT GACACACTCC 
ACCGTGCCAG ATGACTTTGA AGGAGTGCTC ACTATTCTAC AGTGGTTATC CTACATGCCA 
AAGAGCAATC AAAGTCCAGT CCCAATAAAG CCTGCCACTG ATCCAGTCGA CAGAGAGATC 
GATTTTGTTC CCACAAAAGC CCCTTATGAC CCTCGCTGGC TGCTCTGTGG ACGACCCCAT 
CCCACTGTGA AGGGAGCATG GCAGAGTGGA TTCTTTGATC ATGGTTCATT TTTGGAGATA 
ATGGCTACAT GGGCACAGAC AGTGGTGGTG GGCCGAGCCA GGCTTGGTGG GATTCCTCTT 
GGGGTCATTG CTGTGGAGAC CCGCACAGTC GAGCTTGCCA TTCCAGCAGA TCCTGCCAAC 
TTAGACTCAG AGGCCAAGCT CCTCCAGCAA GCTGGTCAGG TGTGGTTTCC AGATTCTGCA 
TATAAAACGG CTCAGGCTAT TGAGGATTTC AATAGAGAGA AACTTCCCCT TATGGTGTTT 
GCCAACTGGC GAGGATTTTC TGGAGGCATG AAAGATATGT ACGACCAGGT GCTCAAGTTT 
GGCTCTTATA TCGTGGATGC CCTGCGAGAA TTCAGCCAGC CTGTGCTGGT TTACATTCCT 
CCTAATGCTG AGCTGAGAGG AGGATCATGG GTCGTGATCG ACCCTACTAT AAACCTTCAG 
CACATGGAGC TCTATGCAGA TCGAGAAAGC AGAGGGGGCG TTTTGGAGGC TGAGGGCACA 
GTGGAGATCA AATTCAGAAA AAAAGACCTG CTAAAGACCA TGCGAAGGAT TGATCCAGTG 
TATTCTCGTC TGGCTGAGCA GCTAGGCAAA CCAGACTTAC CGAGCCAGGA GCGTAAAGAC 
CTGGAGGCAA AACTAAAGTC TAGAGAGGAG TTCCTTCTTC CCATCTACCA CCAGGTGGCA 
GTGCAGTTTG TTGACCTTCA TGACACTCCA GGACGGATGC AGGAAAAGGG AGTCATCACG 
GACATCCTGG ACTGGAAGAC TGCTAGGTCA TTCTTTTACT GGCGTCTGAG GCGACTGCTG 
TTGGAGGAAG TGGTAAAGAA GGAGATCATG CAGGCCAACC AAGACCTGAG CAACGGACAC 
ATACAGTCCA TGCTGCGCCG CTGGTTTGTG GAGACAGAAG GAGCTGTAAA AGCATATCTG 
TGGGACAATA ACAAAGTGGT GGTGGAGTGG CTGGAAAACA ACCTGTCCCA GCAGGACGGA 
ACACGTTCGG TCATCAGAGA AAACATCAAA TGCATAAAGA GGGACTACGC TCTCAAACAC 
ATCCGCAGCT TAGTCCAAGC CAATCCAGAA GTGACTATGG ACTGCATCAT TCACATGGCA 
CAGAACATTA CGCCTTCACA GAGAGCCAAA GTCTGTCACC TGCTGGCCAC CATGGACAAC 
TCCACCACCA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299652.1
CDS	268..7362
Translation	MPSQTPISDCAGEHSPPMDPGLSSTSPRNEGIPPSATSDPTDQSNITVSHIESHRPRFGSGAQAKEKLKFILGASEDNSSDDEPLVKNQTTTHQPKNTETLNAEPTASSEMPPTPVPSTGLRPSMSGPHLLKKGKEHRKMEVHRDFTVASPAEFVTRFGGNRIIDKVLIANNGIAAVKCMRSIRRWSYEMFRNERTIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVEMIVDIAKRIPVQAVWAGWGHASENPKLPELLHKSGICFLGPSSKAMWALGDKVASSIVAQSAGIPTLPWSGTGLSVEWAEEEQRQGRLISVPPELYVQGCVKDVDEGLASAEKIGYPVVIKASEGGGGKGIRKVESSEDFPSLFRQVQAEVPGSPIFIMQLAEHARHLEVQILADQYGNAISLFGRDCSIQRRHQKIIEEAPASIASTITFEQMEQYAVRLAKMVGYVSAGTVEYLFSEDGSFHFLELNPRLQVEHPCTEMIADVNLPAAQLQIAMGIPLYRIKDIRVLFGEAPWGDTTINFESPECMPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSSKNVWGYFSVGATGGLHEFADSQFGHCFSWGENREEAISNMVVAMKELSIRGDFRTTVEYLIKLLETESFRNNDIDTGWLDHLIADKVQAERPDTMLGVVCGALQVADASFRESMSDFLHSLERGQVLPAASLVNTVNVDLIYDGVKYCLKVARQSPTTYVIIMNDSDIEVDVHRLSDGGLLLSYGGSSYTTYMKEEIDRYRVTVGNKTCVFEKERDPTVLRSPSAGKLLQYVVTDGSHVSASQPYAEIEVMKMVMTLHVQHSGCIRFLKRPGTVLEPGCIVALMDLDDPSCIHQVKPNTEPLPAQEPLPMVGERLHQVFHNVLENLVKIMDGYCLPEPYFSQKLKNWVDTLMKTLRDPSLPLLELQEIMTSVAGRIPVTVEKAIRKVMAQYASNITSVLCQFPSQRIANILDSHAATLQRKADREIFFMNTQSIVQLVQRYRSGIRGYMKSVVLDLLKHYLQVEMQFQQAHYDKCVINLREQYKPDMTPVLECIFSHAQVSKKNILVTMLIDQLCGRDPMLADELMVILDELTQLSKMENSKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCPENLKKLILSETSIFDVLPSFFYHNNRVVCMAALEVYVRRAYIAYELNSLQHHQLQDGTCAVDFQFMLPSSHPNRLTLPVNDSGEFPAMRRQGSELFLEGALSPPCQRMGAMVAFHSFDHFKRCFDEVICRFVDPLCESSLFFDGCSTFCDGESCKNMKENPIHIINVSIKQADTEDDDALVTAFTAFAHSKKSLLYDYGIRRVTFLVAQKREFPKYFTFRARDEFHEDRIYRNLEPALAFQLELNRMRNFDLKAVPCAHHRMQLYLGAARVEEGAEVTDYRFFIRAIIRHSDLITKEASFEYLQNEGERLLLEAMDELEVAFSNTPTRTDCNHIFLNFVPTVIMDPYKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTTTGDVIPIRLFLTNESGYYLDISLYKEVNDPSSGQIMFQSYGDKQGPLNGMLINTPYVTKDLLQAKRFQAQSLGTTYVYDFPEMFRQALFKLWGPGDSYPKDVLMCNELVLDSQGNLVQMNRLPGDNEVGMVAFRMRMKTPEYPEGRDIIVICNDITHMIGSFGPQEDQLFMRASELARAEGIPRIYISANSGARIGLAEEIRHMFQVAWIDPEDPYKGFKYLYLTPQDYTRISSSNSVHCHHVEEGGESRYILTDIIGKEEGIGVENLRGSGTIAGETSQAYKEIITISMVTCRAIGIGAYLVRLGQRVIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHSTVPDDFEGVLTILQWLSYMPKSNQSPVPIKPATDPVDREIDFVPTKAPYDPRWLLCGRPHPTVKGAWQSGFFDHGSFLEIMATWAQTVVVGRARLGGIPLGVIAVETRTVELAIPADPANLDSEAKLLQQAGQVWFPDSAYKTAQAIEDFNREKLPLMVFANWRGFSGGMKDMYDQVLKFGSYIVDALREFSQPVLVYIPPNAELRGGSWVVIDPTINLQHMELYADRESRGGVLEAEGTVEIKFRKKDLLKTMRRIDPVYSRLAEQLGKPDLPSQERKDLEAKLKSREEFLLPIYHQVAVQFVDLHDTPGRMQEKGVITDILDWKTARSFFYWRLRRLLLEEVVKKEIMQANQDLSNGHIQSMLRRWFVETEGAVKAYLWDNNKVVVEWLENNLSQQDGTRSVIRENIKCIKRDYALKHIRSLVQANPEVTMDCIIHMAQNITPSQRAKVCHLLATMDNSTTS

Target ORF information:

RefSeq Version	XM_009301377.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase beta (acacb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301377.2

ORF Insert Sequence:

ATGCCATCAC AAACACCCAT CTCAGATTGT GCAGGGGAAC ATTCTCCACC AATGGACCCC 
GGATTATCAA GCACTTCACC AAGAAACGAG GGAATTCCTC CATCAGCAAC TAGCGATCCC 
ACTGACCAAT CAAATATCAC AGTTTCCCAT ATTGAATCCC ACAGGCCCAG GTTTGGTTCT 
GGTGCTCAGG CTAAAGAAAA ACTGAAGTTT ATTTTGGGTG CTTCTGAAGA TAATTCCTCT 
GATGATGAGC CTCTGGTTAA GAACCAAACC ACCACACATC AGCCCAAAAA CACAGAGACA 
CTTAATGCTG AACCTACCGC ATCATCAGAA ATGCCGCCAA CACCAGTTCC ATCGACGGGT 
TTAAGGCCTA GCATGTCAGG CCCCCACCTG CTGAAAAAGG GCAAAGAACA CAGGAAGATG 
GAGGTGCATA GAGATTTCAC CGTTGCCTCT CCAGCGGAGT TTGTCACTCG CTTTGGAGGA 
AACCGCATTA TAGATAAGGT GCTGATTGCA AATAATGGCA TTGCTGCAGT TAAGTGCATG 
CGGTCAATTC GCCGCTGGTC CTATGAAATG TTTCGTAATG AGAGAACCAT TCGCTTTGTG 
GTGATGGTCA CACCCGAAGA CTTGAAAGCC AATGCAGAAT ACATTAAAAT GGCTGATCAC 
TATGTGCCAG TACCAGGGGG TCCAAATAAC AACAACTATG CCAATGTTGA GATGATTGTG 
GATATTGCAA AAAGGATACC AGTGCAGGCT GTGTGGGCTG GATGGGGACA TGCATCTGAG 
AACCCCAAAC TACCAGAGCT CCTTCATAAA TCAGGGATAT GTTTTCTAGG ACCGTCCAGT 
AAAGCCATGT GGGCATTAGG AGATAAAGTG GCATCTTCAA TCGTAGCTCA GAGCGCAGGC 
ATTCCCACGT TGCCCTGGAG TGGAACTGGT CTGAGTGTTG AATGGGCAGA AGAAGAGCAG 
AGGCAGGGTC GTTTGATCAG CGTTCCTCCT GAACTATACG TGCAGGGTTG TGTTAAAGAT 
GTGGACGAGG GACTAGCGAG TGCAGAAAAG ATTGGCTACC CTGTGGTCAT CAAAGCATCA 
GAGGGAGGTG GAGGTAAAGG CATAAGAAAA GTTGAAAGTT CTGAAGACTT TCCTAGCCTT 
TTCAGACAGG TGCAGGCTGA GGTGCCAGGT TCACCCATCT TCATAATGCA ACTAGCCGAA 
CACGCACGCC ACCTGGAGGT GCAGATCTTG GCCGACCAAT ATGGCAATGC CATCTCTCTG 
TTTGGCAGAG ACTGTTCTAT CCAGCGCCGT CACCAGAAGA TCATAGAAGA AGCTCCTGCC 
AGCATTGCCT CCACCATCAC TTTTGAGCAA ATGGAGCAGT ATGCAGTTCG TCTGGCTAAA 
ATGGTGGGCT ATGTGAGCGC TGGTACGGTG GAATACCTGT TTTCTGAGGA TGGAAGTTTC 
CACTTCCTGG AGCTGAATCC TCGACTTCAG GTGGAGCATC CTTGTACGGA GATGATTGCT 
GACGTCAACC TTCCTGCAGC ACAGCTACAG ATAGCGATGG GGATTCCTCT TTACAGAATT 
AAGGATATCC GTGTCCTGTT TGGTGAAGCT CCATGGGGAG ACACAACTAT TAACTTTGAA 
TCTCCAGAAT GCATGCCCTG TCCACGGGGA CACGTCATAG CTGCACGCAT CACCAGTGAA 
AACCCGGATG AGGGCTTTAA ACCAAGCTCA GGTACAGTTC AGGAGCTGAA CTTCCGTAGC 
AGTAAGAATG TATGGGGCTA CTTTAGTGTA GGTGCAACTG GAGGCCTACA TGAGTTTGCA 
GACTCTCAGT TTGGACACTG TTTCTCCTGG GGCGAGAACC GAGAAGAGGC CATCTCGAAC 
ATGGTGGTAG CCATGAAGGA GCTGTCAATC AGAGGAGACT TCAGGACAAC AGTGGAGTAC 
CTTATAAAAC TGCTGGAAAC AGAAAGTTTC AGAAACAATG ACATTGATAC TGGTTGGCTG 
GATCACCTTA TTGCTGATAA AGTGCAGGCA GAGAGGCCAG ATACTATGTT AGGTGTGGTA 
TGCGGAGCTC TACAAGTGGC TGATGCAAGT TTTAGAGAAA GCATGTCTGA CTTCCTGCAT 
TCACTGGAAA GGGGTCAGGT GTTGCCGGCT GCTAGTTTGG TCAACACAGT TAATGTTGAT 
CTGATCTATG ATGGCGTCAA ATACTGCCTG AAGGTAGCCC GTCAGTCACC TACGACATAT 
GTTATCATAA TGAATGACTC CGATATAGAA GTTGATGTCC ATAGGCTCAG TGATGGTGGT 
CTACTGCTTT CCTATGGCGG CAGCAGCTAC ACAACCTACA TGAAGGAGGA GATTGATAGG 
TACCGTGTCA CAGTGGGCAA TAAAACGTGC GTGTTTGAGA AGGAGCGAGA CCCTACGGTG 
CTCCGTTCGC CCTCTGCTGG CAAGCTTTTG CAGTATGTAG TCACTGATGG TTCACATGTT 
TCGGCTTCCC AGCCTTATGC TGAAATTGAG GTGATGAAGA TGGTGATGAC CCTGCATGTC 
CAGCATTCTG GTTGTATTCG CTTTTTAAAA AGACCAGGAA CAGTATTGGA GCCTGGCTGT 
ATAGTGGCCC TAATGGACCT GGATGACCCC AGCTGTATTC ATCAGGTGAA ACCAAACACA 
GAGCCATTAC CAGCTCAGGA GCCATTACCC ATGGTAGGAG AGAGGCTTCA CCAGGTGTTC 
CACAATGTTC TGGAGAACCT GGTGAAAATT ATGGATGGAT ATTGTCTGCC AGAGCCATAT 
TTTAGTCAGA AATTGAAGAA CTGGGTCGAC ACGCTGATGA AGACGCTTCG GGATCCGTCT 
CTGCCTCTAC TTGAACTGCA GGAGATCATG ACCAGCGTGG CAGGGCGAAT TCCAGTTACT 
GTGGAGAAGG CAATTCGAAA GGTCATGGCA CAGTACGCCA GTAACATTAC CTCTGTGCTC 
TGTCAGTTCC CCAGCCAAAG GATAGCAAAT ATACTTGATA GCCATGCTGC CACACTTCAA 
AGGAAAGCAG ACCGGGAAAT TTTCTTCATG AACACCCAGA GCATTGTGCA ATTGGTGCAA 
AGATACCGTA GCGGCATTAG GGGCTACATG AAATCTGTTG TTCTGGATCT TTTGAAACAC 
TACCTTCAGG TGGAGATGCA ATTTCAGCAA GCTCACTATG ACAAGTGTGT GATCAATCTC 
AGAGAGCAGT ATAAGCCGGA CATGACCCCA GTGCTGGAGT GCATCTTTTC TCATGCTCAG 
GTGTCCAAGA AGAACATCCT CGTCACCATG CTCATAGACC AGCTGTGTGG CCGGGACCCA 
ATGCTTGCTG ATGAGCTCAT GGTCATTCTG GATGAGCTTA CACAACTCAG TAAAATGGAA 
AACTCTAAAG TGGCACTTAG GGCCAGACAG GTGTTGATCG CCTCTCATTT GCCCTCATAT 
GAACTCAGGC ACAACCAGGT GGAATCCATC TTTTTATCAG CTATTGATAT GTATGGGCAT 
CAGTTTTGTC CTGAAAACCT CAAGAAACTA ATCCTATCTG AGACCTCCAT CTTTGACGTC 
TTGCCCAGTT TTTTCTACCA CAACAACCGT GTGGTTTGCA TGGCTGCATT AGAGGTTTAT 
GTACGGAGGG CTTATATAGC TTACGAGCTA AACAGTCTTC AGCACCATCA GCTGCAGGAT 
GGGACATGTG CGGTGGATTT CCAGTTCATG TTACCATCAT CCCACCCAAA CAGACTGACC 
CTTCCTGTCA ACGATTCGGG AGAGTTTCCG GCAATGCGCC GTCAAGGCAG TGAGCTCTTT 
CTAGAGGGGG CGCTTTCACC CCCCTGCCAG AGGATGGGAG CCATGGTTGC CTTCCATAGC 
TTTGACCACT TCAAAAGGTG CTTTGATGAA GTGATCTGTC GCTTTGTGGA TCCGCTGTGT 
GAAAGTTCTC TGTTTTTTGA TGGCTGTTCC ACCTTCTGTG ATGGGGAGAG CTGTAAAAAC 
ATGAAGGAAA ACCCCATTCA CATCATAAAT GTGTCAATTA AACAAGCAGA CACAGAAGAT 
GATGATGCTT TGGTCACAGC TTTCACTGCC TTTGCTCACT CCAAAAAATC GCTACTCTAT 
GATTACGGAA TCAGAAGGGT GACATTTTTA GTTGCACAAA AGCGGGAGTT CCCAAAATAC 
TTCACGTTTA GGGCCAGAGA TGAATTCCAT GAAGACAGAA TCTACCGTAA CCTGGAGCCT 
GCTCTGGCCT TCCAGCTGGA GCTGAACCGC ATGCGTAACT TTGACCTTAA GGCTGTTCCC 
TGTGCTCACC ACCGCATGCA GCTGTACTTG GGTGCTGCTC GTGTGGAAGA GGGCGCAGAG 
GTCACTGATT ATCGCTTCTT TATCAGAGCC ATCATTCGCC ACTCTGACCT CATCACCAAG 
GAGGCCTCCT TTGAGTATTT GCAGAATGAG GGTGAGAGGC TGTTGCTGGA GGCGATGGAT 
GAGCTGGAGG TGGCCTTTAG TAACACACCC ACTCGGACTG ACTGCAATCA CATCTTCCTC 
AATTTTGTAC CCACTGTTAT TATGGACCCA TACAAGATAG AGGAGTCTGT CCGCTCCATG 
GTGATGCGAT ACGGTAGTCG ACTGTGGAAG CTGCGAGTTC TCCAGGCTGA GCTGAAAATC 
AACATCCGCC TCACCACCAC TGGAGATGTT ATTCCCATAC GCCTCTTTCT CACCAATGAG 
TCTGGATACT ATCTGGACAT CAGCTTGTAT AAAGAGGTCA ATGACCCATC CAGTGGACAG 
ATAATGTTCC AGTCGTATGG AGACAAACAA GGCCCGCTGA ACGGCATGCT CATAAACACA 
CCATACGTCA CAAAGGATTT ACTCCAGGCC AAGCGCTTCC AGGCTCAGAG CCTCGGCACC 
ACATATGTCT ATGACTTCCC TGAGATGTTT AGACAGGCTT TGTTTAAGCT TTGGGGACCA 
GGAGACAGTT ATCCTAAAGA TGTGTTGATG TGTAATGAGC TGGTCCTGGA TTCTCAGGGG 
AACCTTGTGC AGATGAACAG ACTACCAGGA GACAATGAGG TTGGAATGGT AGCTTTCCGG 
ATGAGGATGA AGACTCCAGA GTACCCAGAG GGCCGAGATA TCATTGTGAT CTGTAATGAT 
ATTACCCATA TGATTGGCTC TTTTGGGCCT CAGGAGGATC AGCTTTTTAT GCGAGCATCT 
GAGCTGGCCA GAGCGGAGGG CATCCCGCGC ATCTACATCT CAGCCAACAG CGGAGCTCGA 
ATCGGCCTGG CAGAGGAGAT CCGCCACATG TTTCAGGTGG CTTGGATCGA CCCTGAAGAC 
CCATATAAGG GTTTTAAGTA CCTGTATCTG ACTCCTCAAG ACTACACCCG CATCAGTTCC 
TCCAATTCTG TCCATTGTCA CCATGTAGAG GAGGGAGGAG AGTCCAGATA CATCCTCACA 
GACATCATAG GAAAGGAAGA GGGTATTGGA GTGGAGAATC TGAGGGGCTC GGGCACAATA 
GCTGGAGAAA CTTCTCAGGC TTATAAAGAA ATAATCACTA TCAGCATGGT CACGTGTCGG 
GCTATAGGAA TCGGTGCCTA TCTGGTACGT CTAGGACAAA GAGTCATTCA AGTGGAAAAC 
TCCCATATCA TCCTGACTGG AGCCGGGGCA TTAAACAAGG TGCTGGGTCG TGAAGTCTAC 
ACCTCCAATA ACCAGCTTGG TGGGGTTCAG ATCATGCACA ACAATGGAGT GACACACTCC 
ACCGTGCCAG ATGACTTTGA AGGAGTGCTC ACTATTCTAC AGTGGTTATC CTACATGCCA 
AAGAGCAATC AAAGTCCAGT CCCAATAAAG CCTGCCACTG ATCCAGTCGA CAGAGAGATC 
GATTTTGTTC CCACAAAAGC CCCTTATGAC CCTCGCTGGC TGCTCTGTGG ACGACCCCAT 
CCCACTGTGA AGGGAGCATG GCAGAGTGGA TTCTTTGATC ATGGTTCATT TTTGGAGATA 
ATGGCTACAT GGGCACAGAC AGTGGTGGTG GGCCGAGCCA GGCTTGGTGG GATTCCTCTT 
GGGGTCATTG CTGTGGAGAC CCGCACAGTC GAGCTTGCCA TTCCAGCAGA TCCTGCCAAC 
TTAGACTCAG AGGCCAAGCT CCTCCAGCAA GCTGGTCAGG TGTGGTTTCC AGATTCTGCA 
TATAAAACGG CTCAGGCTAT TGAGGATTTC AATAGAGAGA AACTTCCCCT TATGGTGTTT 
GCCAACTGGC GAGGATTTTC TGGAGGCATG AAAGATATGT ACGACCAGGT GCTCAAGTTT 
GGCTCTTATA TCGTGGATGC CCTGCGAGAA TTCAGCCAGC CTGTGCTGGT TTACATTCCT 
CCTAATGCTG AGCTGAGAGG AGGATCATGG GTCGTGATCG ACCCTACTAT AAACCTTCAG 
CACATGGAGC TCTATGCAGA TCGAGAAAGC AGAGGGGGCG TTTTGGAGGC TGAGGGCACA 
GTGGAGATCA AATTCAGAAA AAAAGACCTG CTAAAGACCA TGCGAAGGAT TGATCCAGTG 
TATTCTCGTC TGGCTGAGCA GCTAGGCAAA CCAGACTTAC CGAGCCAGGA GCGTAAAGAC 
CTGGAGGCAA AACTAAAGTC TAGAGAGGAG TTCCTTCTTC CCATCTACCA CCAGGTGGCA 
GTGCAGTTTG TTGACCTTCA TGACACTCCA GGACGGATGC AGGAAAAGGG AGTCATCACG 
GACATCCTGG ACTGGAAGAC TGCTAGGTCA TTCTTTTACT GGCGTCTGAG GCGACTGCTG 
TTGGAGGAAG TGGTAAAGAA GGAGATCATG CAGGCCAACC AAGACCTGAG CAACGGACAC 
ATACAGTCCA TGCTGCGCCG CTGGTTTGTG GAGACAGAAG GAGCTGTAAA AGCATATCTG 
TGGGACAATA ACAAAGTGGT GGTGGAGTGG CTGGAAAACA ACCTGTCCCA GCAGGACGGA 
ACACGTTCGG TCATCAGAGA AAACATCAAA TGCATAAAGA GGGACTACGC TCTCAAACAC 
ATCCGCAGCT TAGTCCAAGC CAATCCAGAA GTGACTATGG ACTGCATCAT TCACATGGCA 
CAGAACATTA CGCCTTCACA GAGAGCCAAA GTCTGTCACC TGCTGGCCAC CATGGACAAC 
TCCACCACCA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acacb ( XM_021476434.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332109.1 Danio rerio acetyl-CoA carboxylase beta (acacb), transcript variant X3, mRNA.

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CloneID	ODa10315
Clone ID Related Accession (Same CDS sequence)	XM_009301377.2 , XM_021476434.1
Accession Version	XM_021476434.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	acetyl-CoA carboxylase 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCATCAC AAACACCCAT CTCAGATTGT GCAGGGGAAC ATTCTCCACC AATGGACCCC 
GGATTATCAA GCACTTCACC AAGAAACGAG GGAATTCCTC CATCAGCAAC TAGCGATCCC 
ACTGACCAAT CAAATATCAC AGTTTCCCAT ATTGAATCCC ACAGGCCCAG GTTTGGTTCT 
GGTGCTCAGG CTAAAGAAAA ACTGAAGTTT ATTTTGGGTG CTTCTGAAGA TAATTCCTCT 
GATGATGAGC CTCTGGTTAA GAACCAAACC ACCACACATC AGCCCAAAAA CACAGAGACA 
CTTAATGCTG AACCTACCGC ATCATCAGAA ATGCCGCCAA CACCAGTTCC ATCGACGGGT 
TTAAGGCCTA GCATGTCAGG CCCCCACCTG CTGAAAAAGG GCAAAGAACA CAGGAAGATG 
GAGGTGCATA GAGATTTCAC CGTTGCCTCT CCAGCGGAGT TTGTCACTCG CTTTGGAGGA 
AACCGCATTA TAGATAAGGT GCTGATTGCA AATAATGGCA TTGCTGCAGT TAAGTGCATG 
CGGTCAATTC GCCGCTGGTC CTATGAAATG TTTCGTAATG AGAGAACCAT TCGCTTTGTG 
GTGATGGTCA CACCCGAAGA CTTGAAAGCC AATGCAGAAT ACATTAAAAT GGCTGATCAC 
TATGTGCCAG TACCAGGGGG TCCAAATAAC AACAACTATG CCAATGTTGA GATGATTGTG 
GATATTGCAA AAAGGATACC AGTGCAGGCT GTGTGGGCTG GATGGGGACA TGCATCTGAG 
AACCCCAAAC TACCAGAGCT CCTTCATAAA TCAGGGATAT GTTTTCTAGG ACCGTCCAGT 
AAAGCCATGT GGGCATTAGG AGATAAAGTG GCATCTTCAA TCGTAGCTCA GAGCGCAGGC 
ATTCCCACGT TGCCCTGGAG TGGAACTGGT CTGAGTGTTG AATGGGCAGA AGAAGAGCAG 
AGGCAGGGTC GTTTGATCAG CGTTCCTCCT GAACTATACG TGCAGGGTTG TGTTAAAGAT 
GTGGACGAGG GACTAGCGAG TGCAGAAAAG ATTGGCTACC CTGTGGTCAT CAAAGCATCA 
GAGGGAGGTG GAGGTAAAGG CATAAGAAAA GTTGAAAGTT CTGAAGACTT TCCTAGCCTT 
TTCAGACAGG TGCAGGCTGA GGTGCCAGGT TCACCCATCT TCATAATGCA ACTAGCCGAA 
CACGCACGCC ACCTGGAGGT GCAGATCTTG GCCGACCAAT ATGGCAATGC CATCTCTCTG 
TTTGGCAGAG ACTGTTCTAT CCAGCGCCGT CACCAGAAGA TCATAGAAGA AGCTCCTGCC 
AGCATTGCCT CCACCATCAC TTTTGAGCAA ATGGAGCAGT ATGCAGTTCG TCTGGCTAAA 
ATGGTGGGCT ATGTGAGCGC TGGTACGGTG GAATACCTGT TTTCTGAGGA TGGAAGTTTC 
CACTTCCTGG AGCTGAATCC TCGACTTCAG GTGGAGCATC CTTGTACGGA GATGATTGCT 
GACGTCAACC TTCCTGCAGC ACAGCTACAG ATAGCGATGG GGATTCCTCT TTACAGAATT 
AAGGATATCC GTGTCCTGTT TGGTGAAGCT CCATGGGGAG ACACAACTAT TAACTTTGAA 
TCTCCAGAAT GCATGCCCTG TCCACGGGGA CACGTCATAG CTGCACGCAT CACCAGTGAA 
AACCCGGATG AGGGCTTTAA ACCAAGCTCA GGTACAGTTC AGGAGCTGAA CTTCCGTAGC 
AGTAAGAATG TATGGGGCTA CTTTAGTGTA GGTGCAACTG GAGGCCTACA TGAGTTTGCA 
GACTCTCAGT TTGGACACTG TTTCTCCTGG GGCGAGAACC GAGAAGAGGC CATCTCGAAC 
ATGGTGGTAG CCATGAAGGA GCTGTCAATC AGAGGAGACT TCAGGACAAC AGTGGAGTAC 
CTTATAAAAC TGCTGGAAAC AGAAAGTTTC AGAAACAATG ACATTGATAC TGGTTGGCTG 
GATCACCTTA TTGCTGATAA AGTGCAGGCA GAGAGGCCAG ATACTATGTT AGGTGTGGTA 
TGCGGAGCTC TACAAGTGGC TGATGCAAGT TTTAGAGAAA GCATGTCTGA CTTCCTGCAT 
TCACTGGAAA GGGGTCAGGT GTTGCCGGCT GCTAGTTTGG TCAACACAGT TAATGTTGAT 
CTGATCTATG ATGGCGTCAA ATACTGCCTG AAGGTAGCCC GTCAGTCACC TACGACATAT 
GTTATCATAA TGAATGACTC CGATATAGAA GTTGATGTCC ATAGGCTCAG TGATGGTGGT 
CTACTGCTTT CCTATGGCGG CAGCAGCTAC ACAACCTACA TGAAGGAGGA GATTGATAGG 
TACCGTGTCA CAGTGGGCAA TAAAACGTGC GTGTTTGAGA AGGAGCGAGA CCCTACGGTG 
CTCCGTTCGC CCTCTGCTGG CAAGCTTTTG CAGTATGTAG TCACTGATGG TTCACATGTT 
TCGGCTTCCC AGCCTTATGC TGAAATTGAG GTGATGAAGA TGGTGATGAC CCTGCATGTC 
CAGCATTCTG GTTGTATTCG CTTTTTAAAA AGACCAGGAA CAGTATTGGA GCCTGGCTGT 
ATAGTGGCCC TAATGGACCT GGATGACCCC AGCTGTATTC ATCAGGTGAA ACCAAACACA 
GAGCCATTAC CAGCTCAGGA GCCATTACCC ATGGTAGGAG AGAGGCTTCA CCAGGTGTTC 
CACAATGTTC TGGAGAACCT GGTGAAAATT ATGGATGGAT ATTGTCTGCC AGAGCCATAT 
TTTAGTCAGA AATTGAAGAA CTGGGTCGAC ACGCTGATGA AGACGCTTCG GGATCCGTCT 
CTGCCTCTAC TTGAACTGCA GGAGATCATG ACCAGCGTGG CAGGGCGAAT TCCAGTTACT 
GTGGAGAAGG CAATTCGAAA GGTCATGGCA CAGTACGCCA GTAACATTAC CTCTGTGCTC 
TGTCAGTTCC CCAGCCAAAG GATAGCAAAT ATACTTGATA GCCATGCTGC CACACTTCAA 
AGGAAAGCAG ACCGGGAAAT TTTCTTCATG AACACCCAGA GCATTGTGCA ATTGGTGCAA 
AGATACCGTA GCGGCATTAG GGGCTACATG AAATCTGTTG TTCTGGATCT TTTGAAACAC 
TACCTTCAGG TGGAGATGCA ATTTCAGCAA GCTCACTATG ACAAGTGTGT GATCAATCTC 
AGAGAGCAGT ATAAGCCGGA CATGACCCCA GTGCTGGAGT GCATCTTTTC TCATGCTCAG 
GTGTCCAAGA AGAACATCCT CGTCACCATG CTCATAGACC AGCTGTGTGG CCGGGACCCA 
ATGCTTGCTG ATGAGCTCAT GGTCATTCTG GATGAGCTTA CACAACTCAG TAAAATGGAA 
AACTCTAAAG TGGCACTTAG GGCCAGACAG GTGTTGATCG CCTCTCATTT GCCCTCATAT 
GAACTCAGGC ACAACCAGGT GGAATCCATC TTTTTATCAG CTATTGATAT GTATGGGCAT 
CAGTTTTGTC CTGAAAACCT CAAGAAACTA ATCCTATCTG AGACCTCCAT CTTTGACGTC 
TTGCCCAGTT TTTTCTACCA CAACAACCGT GTGGTTTGCA TGGCTGCATT AGAGGTTTAT 
GTACGGAGGG CTTATATAGC TTACGAGCTA AACAGTCTTC AGCACCATCA GCTGCAGGAT 
GGGACATGTG CGGTGGATTT CCAGTTCATG TTACCATCAT CCCACCCAAA CAGACTGACC 
CTTCCTGTCA ACGATTCGGG AGAGTTTCCG GCAATGCGCC GTCAAGGCAG TGAGCTCTTT 
CTAGAGGGGG CGCTTTCACC CCCCTGCCAG AGGATGGGAG CCATGGTTGC CTTCCATAGC 
TTTGACCACT TCAAAAGGTG CTTTGATGAA GTGATCTGTC GCTTTGTGGA TCCGCTGTGT 
GAAAGTTCTC TGTTTTTTGA TGGCTGTTCC ACCTTCTGTG ATGGGGAGAG CTGTAAAAAC 
ATGAAGGAAA ACCCCATTCA CATCATAAAT GTGTCAATTA AACAAGCAGA CACAGAAGAT 
GATGATGCTT TGGTCACAGC TTTCACTGCC TTTGCTCACT CCAAAAAATC GCTACTCTAT 
GATTACGGAA TCAGAAGGGT GACATTTTTA GTTGCACAAA AGCGGGAGTT CCCAAAATAC 
TTCACGTTTA GGGCCAGAGA TGAATTCCAT GAAGACAGAA TCTACCGTAA CCTGGAGCCT 
GCTCTGGCCT TCCAGCTGGA GCTGAACCGC ATGCGTAACT TTGACCTTAA GGCTGTTCCC 
TGTGCTCACC ACCGCATGCA GCTGTACTTG GGTGCTGCTC GTGTGGAAGA GGGCGCAGAG 
GTCACTGATT ATCGCTTCTT TATCAGAGCC ATCATTCGCC ACTCTGACCT CATCACCAAG 
GAGGCCTCCT TTGAGTATTT GCAGAATGAG GGTGAGAGGC TGTTGCTGGA GGCGATGGAT 
GAGCTGGAGG TGGCCTTTAG TAACACACCC ACTCGGACTG ACTGCAATCA CATCTTCCTC 
AATTTTGTAC CCACTGTTAT TATGGACCCA TACAAGATAG AGGAGTCTGT CCGCTCCATG 
GTGATGCGAT ACGGTAGTCG ACTGTGGAAG CTGCGAGTTC TCCAGGCTGA GCTGAAAATC 
AACATCCGCC TCACCACCAC TGGAGATGTT ATTCCCATAC GCCTCTTTCT CACCAATGAG 
TCTGGATACT ATCTGGACAT CAGCTTGTAT AAAGAGGTCA ATGACCCATC CAGTGGACAG 
ATAATGTTCC AGTCGTATGG AGACAAACAA GGCCCGCTGA ACGGCATGCT CATAAACACA 
CCATACGTCA CAAAGGATTT ACTCCAGGCC AAGCGCTTCC AGGCTCAGAG CCTCGGCACC 
ACATATGTCT ATGACTTCCC TGAGATGTTT AGACAGGCTT TGTTTAAGCT TTGGGGACCA 
GGAGACAGTT ATCCTAAAGA TGTGTTGATG TGTAATGAGC TGGTCCTGGA TTCTCAGGGG 
AACCTTGTGC AGATGAACAG ACTACCAGGA GACAATGAGG TTGGAATGGT AGCTTTCCGG 
ATGAGGATGA AGACTCCAGA GTACCCAGAG GGCCGAGATA TCATTGTGAT CTGTAATGAT 
ATTACCCATA TGATTGGCTC TTTTGGGCCT CAGGAGGATC AGCTTTTTAT GCGAGCATCT 
GAGCTGGCCA GAGCGGAGGG CATCCCGCGC ATCTACATCT CAGCCAACAG CGGAGCTCGA 
ATCGGCCTGG CAGAGGAGAT CCGCCACATG TTTCAGGTGG CTTGGATCGA CCCTGAAGAC 
CCATATAAGG GTTTTAAGTA CCTGTATCTG ACTCCTCAAG ACTACACCCG CATCAGTTCC 
TCCAATTCTG TCCATTGTCA CCATGTAGAG GAGGGAGGAG AGTCCAGATA CATCCTCACA 
GACATCATAG GAAAGGAAGA GGGTATTGGA GTGGAGAATC TGAGGGGCTC GGGCACAATA 
GCTGGAGAAA CTTCTCAGGC TTATAAAGAA ATAATCACTA TCAGCATGGT CACGTGTCGG 
GCTATAGGAA TCGGTGCCTA TCTGGTACGT CTAGGACAAA GAGTCATTCA AGTGGAAAAC 
TCCCATATCA TCCTGACTGG AGCCGGGGCA TTAAACAAGG TGCTGGGTCG TGAAGTCTAC 
ACCTCCAATA ACCAGCTTGG TGGGGTTCAG ATCATGCACA ACAATGGAGT GACACACTCC 
ACCGTGCCAG ATGACTTTGA AGGAGTGCTC ACTATTCTAC AGTGGTTATC CTACATGCCA 
AAGAGCAATC AAAGTCCAGT CCCAATAAAG CCTGCCACTG ATCCAGTCGA CAGAGAGATC 
GATTTTGTTC CCACAAAAGC CCCTTATGAC CCTCGCTGGC TGCTCTGTGG ACGACCCCAT 
CCCACTGTGA AGGGAGCATG GCAGAGTGGA TTCTTTGATC ATGGTTCATT TTTGGAGATA 
ATGGCTACAT GGGCACAGAC AGTGGTGGTG GGCCGAGCCA GGCTTGGTGG GATTCCTCTT 
GGGGTCATTG CTGTGGAGAC CCGCACAGTC GAGCTTGCCA TTCCAGCAGA TCCTGCCAAC 
TTAGACTCAG AGGCCAAGCT CCTCCAGCAA GCTGGTCAGG TGTGGTTTCC AGATTCTGCA 
TATAAAACGG CTCAGGCTAT TGAGGATTTC AATAGAGAGA AACTTCCCCT TATGGTGTTT 
GCCAACTGGC GAGGATTTTC TGGAGGCATG AAAGATATGT ACGACCAGGT GCTCAAGTTT 
GGCTCTTATA TCGTGGATGC CCTGCGAGAA TTCAGCCAGC CTGTGCTGGT TTACATTCCT 
CCTAATGCTG AGCTGAGAGG AGGATCATGG GTCGTGATCG ACCCTACTAT AAACCTTCAG 
CACATGGAGC TCTATGCAGA TCGAGAAAGC AGAGGGGGCG TTTTGGAGGC TGAGGGCACA 
GTGGAGATCA AATTCAGAAA AAAAGACCTG CTAAAGACCA TGCGAAGGAT TGATCCAGTG 
TATTCTCGTC TGGCTGAGCA GCTAGGCAAA CCAGACTTAC CGAGCCAGGA GCGTAAAGAC 
CTGGAGGCAA AACTAAAGTC TAGAGAGGAG TTCCTTCTTC CCATCTACCA CCAGGTGGCA 
GTGCAGTTTG TTGACCTTCA TGACACTCCA GGACGGATGC AGGAAAAGGG AGTCATCACG 
GACATCCTGG ACTGGAAGAC TGCTAGGTCA TTCTTTTACT GGCGTCTGAG GCGACTGCTG 
TTGGAGGAAG TGGTAAAGAA GGAGATCATG CAGGCCAACC AAGACCTGAG CAACGGACAC 
ATACAGTCCA TGCTGCGCCG CTGGTTTGTG GAGACAGAAG GAGCTGTAAA AGCATATCTG 
TGGGACAATA ACAAAGTGGT GGTGGAGTGG CTGGAAAACA ACCTGTCCCA GCAGGACGGA 
ACACGTTCGG TCATCAGAGA AAACATCAAA TGCATAAAGA GGGACTACGC TCTCAAACAC 
ATCCGCAGCT TAGTCCAAGC CAATCCAGAA GTGACTATGG ACTGCATCAT TCACATGGCA 
CAGAACATTA CGCCTTCACA GAGAGCCAAA GTCTGTCACC TGCTGGCCAC CATGGACAAC 
TCCACCACCA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332109.1
CDS	225..7319
Translation	MPSQTPISDCAGEHSPPMDPGLSSTSPRNEGIPPSATSDPTDQSNITVSHIESHRPRFGSGAQAKEKLKFILGASEDNSSDDEPLVKNQTTTHQPKNTETLNAEPTASSEMPPTPVPSTGLRPSMSGPHLLKKGKEHRKMEVHRDFTVASPAEFVTRFGGNRIIDKVLIANNGIAAVKCMRSIRRWSYEMFRNERTIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVEMIVDIAKRIPVQAVWAGWGHASENPKLPELLHKSGICFLGPSSKAMWALGDKVASSIVAQSAGIPTLPWSGTGLSVEWAEEEQRQGRLISVPPELYVQGCVKDVDEGLASAEKIGYPVVIKASEGGGGKGIRKVESSEDFPSLFRQVQAEVPGSPIFIMQLAEHARHLEVQILADQYGNAISLFGRDCSIQRRHQKIIEEAPASIASTITFEQMEQYAVRLAKMVGYVSAGTVEYLFSEDGSFHFLELNPRLQVEHPCTEMIADVNLPAAQLQIAMGIPLYRIKDIRVLFGEAPWGDTTINFESPECMPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSSKNVWGYFSVGATGGLHEFADSQFGHCFSWGENREEAISNMVVAMKELSIRGDFRTTVEYLIKLLETESFRNNDIDTGWLDHLIADKVQAERPDTMLGVVCGALQVADASFRESMSDFLHSLERGQVLPAASLVNTVNVDLIYDGVKYCLKVARQSPTTYVIIMNDSDIEVDVHRLSDGGLLLSYGGSSYTTYMKEEIDRYRVTVGNKTCVFEKERDPTVLRSPSAGKLLQYVVTDGSHVSASQPYAEIEVMKMVMTLHVQHSGCIRFLKRPGTVLEPGCIVALMDLDDPSCIHQVKPNTEPLPAQEPLPMVGERLHQVFHNVLENLVKIMDGYCLPEPYFSQKLKNWVDTLMKTLRDPSLPLLELQEIMTSVAGRIPVTVEKAIRKVMAQYASNITSVLCQFPSQRIANILDSHAATLQRKADREIFFMNTQSIVQLVQRYRSGIRGYMKSVVLDLLKHYLQVEMQFQQAHYDKCVINLREQYKPDMTPVLECIFSHAQVSKKNILVTMLIDQLCGRDPMLADELMVILDELTQLSKMENSKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCPENLKKLILSETSIFDVLPSFFYHNNRVVCMAALEVYVRRAYIAYELNSLQHHQLQDGTCAVDFQFMLPSSHPNRLTLPVNDSGEFPAMRRQGSELFLEGALSPPCQRMGAMVAFHSFDHFKRCFDEVICRFVDPLCESSLFFDGCSTFCDGESCKNMKENPIHIINVSIKQADTEDDDALVTAFTAFAHSKKSLLYDYGIRRVTFLVAQKREFPKYFTFRARDEFHEDRIYRNLEPALAFQLELNRMRNFDLKAVPCAHHRMQLYLGAARVEEGAEVTDYRFFIRAIIRHSDLITKEASFEYLQNEGERLLLEAMDELEVAFSNTPTRTDCNHIFLNFVPTVIMDPYKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTTTGDVIPIRLFLTNESGYYLDISLYKEVNDPSSGQIMFQSYGDKQGPLNGMLINTPYVTKDLLQAKRFQAQSLGTTYVYDFPEMFRQALFKLWGPGDSYPKDVLMCNELVLDSQGNLVQMNRLPGDNEVGMVAFRMRMKTPEYPEGRDIIVICNDITHMIGSFGPQEDQLFMRASELARAEGIPRIYISANSGARIGLAEEIRHMFQVAWIDPEDPYKGFKYLYLTPQDYTRISSSNSVHCHHVEEGGESRYILTDIIGKEEGIGVENLRGSGTIAGETSQAYKEIITISMVTCRAIGIGAYLVRLGQRVIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHSTVPDDFEGVLTILQWLSYMPKSNQSPVPIKPATDPVDREIDFVPTKAPYDPRWLLCGRPHPTVKGAWQSGFFDHGSFLEIMATWAQTVVVGRARLGGIPLGVIAVETRTVELAIPADPANLDSEAKLLQQAGQVWFPDSAYKTAQAIEDFNREKLPLMVFANWRGFSGGMKDMYDQVLKFGSYIVDALREFSQPVLVYIPPNAELRGGSWVVIDPTINLQHMELYADRESRGGVLEAEGTVEIKFRKKDLLKTMRRIDPVYSRLAEQLGKPDLPSQERKDLEAKLKSREEFLLPIYHQVAVQFVDLHDTPGRMQEKGVITDILDWKTARSFFYWRLRRLLLEEVVKKEIMQANQDLSNGHIQSMLRRWFVETEGAVKAYLWDNNKVVVEWLENNLSQQDGTRSVIRENIKCIKRDYALKHIRSLVQANPEVTMDCIIHMAQNITPSQRAKVCHLLATMDNSTTS

Target ORF information:

RefSeq Version	XM_021476434.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase beta (acacb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476434.1

ORF Insert Sequence:

ATGCCATCAC AAACACCCAT CTCAGATTGT GCAGGGGAAC ATTCTCCACC AATGGACCCC 
GGATTATCAA GCACTTCACC AAGAAACGAG GGAATTCCTC CATCAGCAAC TAGCGATCCC 
ACTGACCAAT CAAATATCAC AGTTTCCCAT ATTGAATCCC ACAGGCCCAG GTTTGGTTCT 
GGTGCTCAGG CTAAAGAAAA ACTGAAGTTT ATTTTGGGTG CTTCTGAAGA TAATTCCTCT 
GATGATGAGC CTCTGGTTAA GAACCAAACC ACCACACATC AGCCCAAAAA CACAGAGACA 
CTTAATGCTG AACCTACCGC ATCATCAGAA ATGCCGCCAA CACCAGTTCC ATCGACGGGT 
TTAAGGCCTA GCATGTCAGG CCCCCACCTG CTGAAAAAGG GCAAAGAACA CAGGAAGATG 
GAGGTGCATA GAGATTTCAC CGTTGCCTCT CCAGCGGAGT TTGTCACTCG CTTTGGAGGA 
AACCGCATTA TAGATAAGGT GCTGATTGCA AATAATGGCA TTGCTGCAGT TAAGTGCATG 
CGGTCAATTC GCCGCTGGTC CTATGAAATG TTTCGTAATG AGAGAACCAT TCGCTTTGTG 
GTGATGGTCA CACCCGAAGA CTTGAAAGCC AATGCAGAAT ACATTAAAAT GGCTGATCAC 
TATGTGCCAG TACCAGGGGG TCCAAATAAC AACAACTATG CCAATGTTGA GATGATTGTG 
GATATTGCAA AAAGGATACC AGTGCAGGCT GTGTGGGCTG GATGGGGACA TGCATCTGAG 
AACCCCAAAC TACCAGAGCT CCTTCATAAA TCAGGGATAT GTTTTCTAGG ACCGTCCAGT 
AAAGCCATGT GGGCATTAGG AGATAAAGTG GCATCTTCAA TCGTAGCTCA GAGCGCAGGC 
ATTCCCACGT TGCCCTGGAG TGGAACTGGT CTGAGTGTTG AATGGGCAGA AGAAGAGCAG 
AGGCAGGGTC GTTTGATCAG CGTTCCTCCT GAACTATACG TGCAGGGTTG TGTTAAAGAT 
GTGGACGAGG GACTAGCGAG TGCAGAAAAG ATTGGCTACC CTGTGGTCAT CAAAGCATCA 
GAGGGAGGTG GAGGTAAAGG CATAAGAAAA GTTGAAAGTT CTGAAGACTT TCCTAGCCTT 
TTCAGACAGG TGCAGGCTGA GGTGCCAGGT TCACCCATCT TCATAATGCA ACTAGCCGAA 
CACGCACGCC ACCTGGAGGT GCAGATCTTG GCCGACCAAT ATGGCAATGC CATCTCTCTG 
TTTGGCAGAG ACTGTTCTAT CCAGCGCCGT CACCAGAAGA TCATAGAAGA AGCTCCTGCC 
AGCATTGCCT CCACCATCAC TTTTGAGCAA ATGGAGCAGT ATGCAGTTCG TCTGGCTAAA 
ATGGTGGGCT ATGTGAGCGC TGGTACGGTG GAATACCTGT TTTCTGAGGA TGGAAGTTTC 
CACTTCCTGG AGCTGAATCC TCGACTTCAG GTGGAGCATC CTTGTACGGA GATGATTGCT 
GACGTCAACC TTCCTGCAGC ACAGCTACAG ATAGCGATGG GGATTCCTCT TTACAGAATT 
AAGGATATCC GTGTCCTGTT TGGTGAAGCT CCATGGGGAG ACACAACTAT TAACTTTGAA 
TCTCCAGAAT GCATGCCCTG TCCACGGGGA CACGTCATAG CTGCACGCAT CACCAGTGAA 
AACCCGGATG AGGGCTTTAA ACCAAGCTCA GGTACAGTTC AGGAGCTGAA CTTCCGTAGC 
AGTAAGAATG TATGGGGCTA CTTTAGTGTA GGTGCAACTG GAGGCCTACA TGAGTTTGCA 
GACTCTCAGT TTGGACACTG TTTCTCCTGG GGCGAGAACC GAGAAGAGGC CATCTCGAAC 
ATGGTGGTAG CCATGAAGGA GCTGTCAATC AGAGGAGACT TCAGGACAAC AGTGGAGTAC 
CTTATAAAAC TGCTGGAAAC AGAAAGTTTC AGAAACAATG ACATTGATAC TGGTTGGCTG 
GATCACCTTA TTGCTGATAA AGTGCAGGCA GAGAGGCCAG ATACTATGTT AGGTGTGGTA 
TGCGGAGCTC TACAAGTGGC TGATGCAAGT TTTAGAGAAA GCATGTCTGA CTTCCTGCAT 
TCACTGGAAA GGGGTCAGGT GTTGCCGGCT GCTAGTTTGG TCAACACAGT TAATGTTGAT 
CTGATCTATG ATGGCGTCAA ATACTGCCTG AAGGTAGCCC GTCAGTCACC TACGACATAT 
GTTATCATAA TGAATGACTC CGATATAGAA GTTGATGTCC ATAGGCTCAG TGATGGTGGT 
CTACTGCTTT CCTATGGCGG CAGCAGCTAC ACAACCTACA TGAAGGAGGA GATTGATAGG 
TACCGTGTCA CAGTGGGCAA TAAAACGTGC GTGTTTGAGA AGGAGCGAGA CCCTACGGTG 
CTCCGTTCGC CCTCTGCTGG CAAGCTTTTG CAGTATGTAG TCACTGATGG TTCACATGTT 
TCGGCTTCCC AGCCTTATGC TGAAATTGAG GTGATGAAGA TGGTGATGAC CCTGCATGTC 
CAGCATTCTG GTTGTATTCG CTTTTTAAAA AGACCAGGAA CAGTATTGGA GCCTGGCTGT 
ATAGTGGCCC TAATGGACCT GGATGACCCC AGCTGTATTC ATCAGGTGAA ACCAAACACA 
GAGCCATTAC CAGCTCAGGA GCCATTACCC ATGGTAGGAG AGAGGCTTCA CCAGGTGTTC 
CACAATGTTC TGGAGAACCT GGTGAAAATT ATGGATGGAT ATTGTCTGCC AGAGCCATAT 
TTTAGTCAGA AATTGAAGAA CTGGGTCGAC ACGCTGATGA AGACGCTTCG GGATCCGTCT 
CTGCCTCTAC TTGAACTGCA GGAGATCATG ACCAGCGTGG CAGGGCGAAT TCCAGTTACT 
GTGGAGAAGG CAATTCGAAA GGTCATGGCA CAGTACGCCA GTAACATTAC CTCTGTGCTC 
TGTCAGTTCC CCAGCCAAAG GATAGCAAAT ATACTTGATA GCCATGCTGC CACACTTCAA 
AGGAAAGCAG ACCGGGAAAT TTTCTTCATG AACACCCAGA GCATTGTGCA ATTGGTGCAA 
AGATACCGTA GCGGCATTAG GGGCTACATG AAATCTGTTG TTCTGGATCT TTTGAAACAC 
TACCTTCAGG TGGAGATGCA ATTTCAGCAA GCTCACTATG ACAAGTGTGT GATCAATCTC 
AGAGAGCAGT ATAAGCCGGA CATGACCCCA GTGCTGGAGT GCATCTTTTC TCATGCTCAG 
GTGTCCAAGA AGAACATCCT CGTCACCATG CTCATAGACC AGCTGTGTGG CCGGGACCCA 
ATGCTTGCTG ATGAGCTCAT GGTCATTCTG GATGAGCTTA CACAACTCAG TAAAATGGAA 
AACTCTAAAG TGGCACTTAG GGCCAGACAG GTGTTGATCG CCTCTCATTT GCCCTCATAT 
GAACTCAGGC ACAACCAGGT GGAATCCATC TTTTTATCAG CTATTGATAT GTATGGGCAT 
CAGTTTTGTC CTGAAAACCT CAAGAAACTA ATCCTATCTG AGACCTCCAT CTTTGACGTC 
TTGCCCAGTT TTTTCTACCA CAACAACCGT GTGGTTTGCA TGGCTGCATT AGAGGTTTAT 
GTACGGAGGG CTTATATAGC TTACGAGCTA AACAGTCTTC AGCACCATCA GCTGCAGGAT 
GGGACATGTG CGGTGGATTT CCAGTTCATG TTACCATCAT CCCACCCAAA CAGACTGACC 
CTTCCTGTCA ACGATTCGGG AGAGTTTCCG GCAATGCGCC GTCAAGGCAG TGAGCTCTTT 
CTAGAGGGGG CGCTTTCACC CCCCTGCCAG AGGATGGGAG CCATGGTTGC CTTCCATAGC 
TTTGACCACT TCAAAAGGTG CTTTGATGAA GTGATCTGTC GCTTTGTGGA TCCGCTGTGT 
GAAAGTTCTC TGTTTTTTGA TGGCTGTTCC ACCTTCTGTG ATGGGGAGAG CTGTAAAAAC 
ATGAAGGAAA ACCCCATTCA CATCATAAAT GTGTCAATTA AACAAGCAGA CACAGAAGAT 
GATGATGCTT TGGTCACAGC TTTCACTGCC TTTGCTCACT CCAAAAAATC GCTACTCTAT 
GATTACGGAA TCAGAAGGGT GACATTTTTA GTTGCACAAA AGCGGGAGTT CCCAAAATAC 
TTCACGTTTA GGGCCAGAGA TGAATTCCAT GAAGACAGAA TCTACCGTAA CCTGGAGCCT 
GCTCTGGCCT TCCAGCTGGA GCTGAACCGC ATGCGTAACT TTGACCTTAA GGCTGTTCCC 
TGTGCTCACC ACCGCATGCA GCTGTACTTG GGTGCTGCTC GTGTGGAAGA GGGCGCAGAG 
GTCACTGATT ATCGCTTCTT TATCAGAGCC ATCATTCGCC ACTCTGACCT CATCACCAAG 
GAGGCCTCCT TTGAGTATTT GCAGAATGAG GGTGAGAGGC TGTTGCTGGA GGCGATGGAT 
GAGCTGGAGG TGGCCTTTAG TAACACACCC ACTCGGACTG ACTGCAATCA CATCTTCCTC 
AATTTTGTAC CCACTGTTAT TATGGACCCA TACAAGATAG AGGAGTCTGT CCGCTCCATG 
GTGATGCGAT ACGGTAGTCG ACTGTGGAAG CTGCGAGTTC TCCAGGCTGA GCTGAAAATC 
AACATCCGCC TCACCACCAC TGGAGATGTT ATTCCCATAC GCCTCTTTCT CACCAATGAG 
TCTGGATACT ATCTGGACAT CAGCTTGTAT AAAGAGGTCA ATGACCCATC CAGTGGACAG 
ATAATGTTCC AGTCGTATGG AGACAAACAA GGCCCGCTGA ACGGCATGCT CATAAACACA 
CCATACGTCA CAAAGGATTT ACTCCAGGCC AAGCGCTTCC AGGCTCAGAG CCTCGGCACC 
ACATATGTCT ATGACTTCCC TGAGATGTTT AGACAGGCTT TGTTTAAGCT TTGGGGACCA 
GGAGACAGTT ATCCTAAAGA TGTGTTGATG TGTAATGAGC TGGTCCTGGA TTCTCAGGGG 
AACCTTGTGC AGATGAACAG ACTACCAGGA GACAATGAGG TTGGAATGGT AGCTTTCCGG 
ATGAGGATGA AGACTCCAGA GTACCCAGAG GGCCGAGATA TCATTGTGAT CTGTAATGAT 
ATTACCCATA TGATTGGCTC TTTTGGGCCT CAGGAGGATC AGCTTTTTAT GCGAGCATCT 
GAGCTGGCCA GAGCGGAGGG CATCCCGCGC ATCTACATCT CAGCCAACAG CGGAGCTCGA 
ATCGGCCTGG CAGAGGAGAT CCGCCACATG TTTCAGGTGG CTTGGATCGA CCCTGAAGAC 
CCATATAAGG GTTTTAAGTA CCTGTATCTG ACTCCTCAAG ACTACACCCG CATCAGTTCC 
TCCAATTCTG TCCATTGTCA CCATGTAGAG GAGGGAGGAG AGTCCAGATA CATCCTCACA 
GACATCATAG GAAAGGAAGA GGGTATTGGA GTGGAGAATC TGAGGGGCTC GGGCACAATA 
GCTGGAGAAA CTTCTCAGGC TTATAAAGAA ATAATCACTA TCAGCATGGT CACGTGTCGG 
GCTATAGGAA TCGGTGCCTA TCTGGTACGT CTAGGACAAA GAGTCATTCA AGTGGAAAAC 
TCCCATATCA TCCTGACTGG AGCCGGGGCA TTAAACAAGG TGCTGGGTCG TGAAGTCTAC 
ACCTCCAATA ACCAGCTTGG TGGGGTTCAG ATCATGCACA ACAATGGAGT GACACACTCC 
ACCGTGCCAG ATGACTTTGA AGGAGTGCTC ACTATTCTAC AGTGGTTATC CTACATGCCA 
AAGAGCAATC AAAGTCCAGT CCCAATAAAG CCTGCCACTG ATCCAGTCGA CAGAGAGATC 
GATTTTGTTC CCACAAAAGC CCCTTATGAC CCTCGCTGGC TGCTCTGTGG ACGACCCCAT 
CCCACTGTGA AGGGAGCATG GCAGAGTGGA TTCTTTGATC ATGGTTCATT TTTGGAGATA 
ATGGCTACAT GGGCACAGAC AGTGGTGGTG GGCCGAGCCA GGCTTGGTGG GATTCCTCTT 
GGGGTCATTG CTGTGGAGAC CCGCACAGTC GAGCTTGCCA TTCCAGCAGA TCCTGCCAAC 
TTAGACTCAG AGGCCAAGCT CCTCCAGCAA GCTGGTCAGG TGTGGTTTCC AGATTCTGCA 
TATAAAACGG CTCAGGCTAT TGAGGATTTC AATAGAGAGA AACTTCCCCT TATGGTGTTT 
GCCAACTGGC GAGGATTTTC TGGAGGCATG AAAGATATGT ACGACCAGGT GCTCAAGTTT 
GGCTCTTATA TCGTGGATGC CCTGCGAGAA TTCAGCCAGC CTGTGCTGGT TTACATTCCT 
CCTAATGCTG AGCTGAGAGG AGGATCATGG GTCGTGATCG ACCCTACTAT AAACCTTCAG 
CACATGGAGC TCTATGCAGA TCGAGAAAGC AGAGGGGGCG TTTTGGAGGC TGAGGGCACA 
GTGGAGATCA AATTCAGAAA AAAAGACCTG CTAAAGACCA TGCGAAGGAT TGATCCAGTG 
TATTCTCGTC TGGCTGAGCA GCTAGGCAAA CCAGACTTAC CGAGCCAGGA GCGTAAAGAC 
CTGGAGGCAA AACTAAAGTC TAGAGAGGAG TTCCTTCTTC CCATCTACCA CCAGGTGGCA 
GTGCAGTTTG TTGACCTTCA TGACACTCCA GGACGGATGC AGGAAAAGGG AGTCATCACG 
GACATCCTGG ACTGGAAGAC TGCTAGGTCA TTCTTTTACT GGCGTCTGAG GCGACTGCTG 
TTGGAGGAAG TGGTAAAGAA GGAGATCATG CAGGCCAACC AAGACCTGAG CAACGGACAC 
ATACAGTCCA TGCTGCGCCG CTGGTTTGTG GAGACAGAAG GAGCTGTAAA AGCATATCTG 
TGGGACAATA ACAAAGTGGT GGTGGAGTGG CTGGAAAACA ACCTGTCCCA GCAGGACGGA 
ACACGTTCGG TCATCAGAGA AAACATCAAA TGCATAAAGA GGGACTACGC TCTCAAACAC 
ATCCGCAGCT TAGTCCAAGC CAATCCAGAA GTGACTATGG ACTGCATCAT TCACATGGCA 
CAGAACATTA CGCCTTCACA GAGAGCCAAA GTCTGTCACC TGCTGGCCAC CATGGACAAC 
TCCACCACCA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acacb ( XM_009301375.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299650.1 Danio rerio acetyl-CoA carboxylase beta (acacb), transcript variant X1, mRNA.

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CloneID	ODa42328
Clone ID Related Accession (Same CDS sequence)	XM_009301375.2 , XM_021476432.1
Accession Version	XM_009301375.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	acetyl-CoA carboxylase 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301375.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCATCAC AAACACCCAT CTCAGATTGT GCAGGGGAAC ATTCTCCACC AATGGACCCC 
GGATTATCAA GCACTTCACC AAGAAACGAG GGAATTCCTC CATCAGCAAC TAGCGATCCC 
ACTGACCAAT CAAATATCAC AGTTTCCCAT ATTGAATCCC ACAGGCCCAG GTTTGGTTCT 
GGTGCTCAGG CTAAAGAAAA ACTGAAGTTT ATTTTGGGTG CTTCTGAAGA TAATTCCTCT 
GATGATGAGC CTCTGGTTAA GAACCAAACC ACCACACATC AGCCCAAAAA CACAGAGACA 
CTTAATGCTG AACCTACCGC ATCATCAGAA ATGCCGCCAA CACCAGTTCC ATCGACGGGT 
TTAAGGCCTA GCATGTCAGG CCCCCACCTG CTGAAAAAGG GCAAAGAACA CAGGAAGATG 
GAGGTGCATA GAGATTTCAC CGTTGCCTCT CCAGCGGAGT TTGTCACTCG CTTTGGAGGA 
AACCGCATTA TAGATAAGGT GCTGATTGCA AATAATGGCA TTGCTGCAGT TAAGTGCATG 
CGGTCAATTC GCCGCTGGTC CTATGAAATG TTTCGTAATG AGAGAACCAT TCGCTTTGTG 
GTGATGGTCA CACCCGAAGA CTTGAAAGCC AATGCAGAAT ACATTAAAAT GGCTGATCAC 
TATGTGCCAG TACCAGGGGG TCCAAATAAC AACAACTATG CCAATGTTGA GATGATTGTG 
GATATTGCAA AAAGGATACC AGTGCAGGCT GTGTGGGCTG GATGGGGACA TGCATCTGAG 
AACCCCAAAC TACCAGAGCT CCTTCATAAA TCAGGGATAT GTTTTCTAGG ACCGTCCAGT 
AAAGCCATGT GGGCATTAGG AGATAAAGTG GCATCTTCAA TCGTAGCTCA GAGCGCAGGC 
ATTCCCACGT TGCCCTGGAG TGGAACTGGT CTGAGTGTTG AATGGGCAGA AGAAGAGCAG 
AGGCAGGGTC GTTTGATCAG CGTTCCTCCT GAACTATACG TGCAGGGTTG TGTTAAAGAT 
GTGGACGAGG GACTAGCGAG TGCAGAAAAG ATTGGCTACC CTGTGGTCAT CAAAGCATCA 
GAGGGAGGTG GAGGTAAAGG CATAAGAAAA GTTGAAAGTT CTGAAGACTT TCCTAGCCTT 
TTCAGACAGG TGCAGGCTGA GGTGCCAGGT TCACCCATCT TCATAATGCA ACTAGCCGAA 
CACGCACGCC ACCTGGAGGT GCAGATCTTG GCCGACCAAT ATGGCAATGC CATCTCTCTG 
TTTGGCAGAG ACTGTTCTAT CCAGCGCCGT CACCAGAAGA TCATAGAAGA AGCTCCTGCC 
AGCATTGCCT CCACCATCAC TTTTGAGCAA ATGGAGCAGT ATGCAGTTCG TCTGGCTAAA 
ATGGTGGGCT ATGTGAGCGC TGGTACGGTG GAATACCTGT TTTCTGAGGA TGGAAGTTTC 
CACTTCCTGG AGCTGAATCC TCGACTTCAG GTGGAGCATC CTTGTACGGA GATGATTGCT 
GACGTCAACC TTCCTGCAGC ACAGCTACAG ATAGCGATGG GGATTCCTCT TTACAGAATT 
AAGGATATCC GTGTCCTGTT TGGTGAAGCT CCATGGGGAG ACACAACTAT TAACTTTGAA 
TCTCCAGAAT GCATGCCCTG TCCACGGGGA CACGTCATAG CTGCACGCAT CACCAGTGAA 
AACCCGGATG AGGGCTTTAA ACCAAGCTCA GGTACAGTTC AGGAGCTGAA CTTCCGTAGC 
AGTAAGAATG TATGGGGCTA CTTTAGTGTA GGTGCAACTG GAGGCCTACA TGAGTTTGCA 
GACTCTCAGT TTGGACACTG TTTCTCCTGG GGCGAGAACC GAGAAGAGGC CATCTCGAAC 
ATGGTGGTAG CCATGAAGGA GCTGTCAATC AGAGGAGACT TCAGGACAAC AGTGGAGTAC 
CTTATAAAAC TGCTGGAAAC AGAAAGTTTC AGAAACAATG ACATTGATAC TGGTTGGCTG 
GATCACCTTA TTGCTGATAA AGTGCAGGCA GAGAGGCCAG ATACTATGTT AGGTGTGGTA 
TGCGGAGCTC TACAAGTGGC TGATGCAAGT TTTAGAGAAA GCATGTCTGA CTTCCTGCAT 
TCACTGGAAA GGGGTCAGGT GTTGCCGGCT GCTAGTTTGG TCAACACAGT TAATGTTGAT 
CTGATCTATG ATGGCGTCAA ATACTGCCTG AAGGTAGCCC GTCAGTCACC TACGACATAT 
GTTATCATAA TGAATGACTC CGATATAGAA GTTGATGTCC ATAGGCTCAG TGATGGTGGT 
CTACTGCTTT CCTATGGCGG CAGCAGCTAC ACAACCTACA TGAAGGAGGA GATTGATAGG 
TACCGTGTCA CAGTGGGCAA TAAAACGTGC GTGTTTGAGA AGGAGCGAGA CCCTACGGTG 
CTCCGTTCGC CCTCTGCTGG CAAGCTTTTG CAGTATGTAG TCACTGATGG TTCACATGTT 
TCGGCTTCCC AGCCTTATGC TGAAATTGAG GTGATGAAGA TGGTGATGAC CCTGCATGTC 
CAGCATTCTG GTTGTATTCG CTTTTTAAAA AGACCAGGAA CAGTATTGGA GCCTGGCTGT 
ATAGTGGCCC TAATGGACCT GGATGACCCC AGCTGTATTC ATCAGGTGAA ACCAAACACA 
GAGCCATTAC CAGCTCAGGA GCCATTACCC ATGGTAGGAG AGAGGCTTCA CCAGGTGTTC 
CACAATGTTC TGGAGAACCT GGTGAAAATT ATGGATGGAT ATTGTCTGCC AGAGCCATAT 
TTTAGTCAGA AATTGAAGAA CTGGGTCGAC ACGCTGATGA AGACGCTTCG GGATCCGTCT 
CTGCCTCTAC TTGAACTGCA GGAGATCATG ACCAGCGTGG CAGGGCGAAT TCCAGTTACT 
GTGGAGAAGG CAATTCGAAA GGTCATGGCA CAGTACGCCA GTAACATTAC CTCTGTGCTC 
TGTCAGTTCC CCAGCCAAAG GATAGCAAAT ATACTTGATA GCCATGCTGC CACACTTCAA 
AGGAAAGCAG ACCGGGAAAT TTTCTTCATG AACACCCAGA GCATTGTGCA ATTGGTGCAA 
AGATACCGTA GCGGCATTAG GGGCTACATG AAATCTGTTG TTCTGGATCT TTTGAAACAC 
TACCTTCAGG TGGAGATGCA ATTTCAGCAA GCTCACTATG ACAAGTGTGT GATCAATCTC 
AGAGAGCAGT ATAAGCCGGA CATGACCCCA GTGCTGGAGT GCATCTTTTC TCATGCTCAG 
GTGTCCAAGA AGAACATCCT CGTCACCATG CTCATAGACC AGCTGTGTGG CCGGGACCCA 
ATGCTTGCTG ATGAGCTCAT GGTCATTCTG GATGAGCTTA CACAACTCAG TAAAATGGAA 
AACTCTAAAG TGGCACTTAG GGCCAGACAG GTGTTGATCG CCTCTCATTT GCCCTCATAT 
GAACTCAGGC ACAACCAGGT GGAATCCATC TTTTTATCAG CTATTGATAT GTATGGGCAT 
CAGTTTTGTC CTGAAAACCT CAAGAAACTA ATCCTATCTG AGACCTCCAT CTTTGACGTC 
TTGCCCAGTT TTTTCTACCA CAACAACCGT GTGGTTTGCA TGGCTGCATT AGAGGTTTAT 
GTACGGAGGG CTTATATAGC TTACGAGCTA AACAGTCTTC AGCACCATCA GCTGCAGGAT 
GGGACATGTG CGGTGGATTT CCAGTTCATG TTACCATCAT CCCACCCAAA CAGAGAGCCT 
AACGCCACAG TGAGCCAGCA CATGCCCAAA TCCACAGGGA GCAGTCCTAC TTTGAACAGA 
CTGACCCTTC CTGTCAACGA TTCGGGAGAG TTTCCGGCAA TGCGCCGTCA AGGCAGTGAG 
CTCTTTCTAG AGGGGGCGCT TTCACCCCCC TGCCAGAGGA TGGGAGCCAT GGTTGCCTTC 
CATAGCTTTG ACCACTTCAA AAGGTGCTTT GATGAAGTGA TCTGTCGCTT TGTGGATCCG 
CTGTGTGAAA GTTCTCTGTT TTTTGATGGC TGTTCCACCT TCTGTGATGG GGAGAGCTGT 
AAAAACATGA AGGAAAACCC CATTCACATC ATAAATGTGT CAATTAAACA AGCAGACACA 
GAAGATGATG ATGCTTTGGT CACAGCTTTC ACTGCCTTTG CTCACTCCAA AAAATCGCTA 
CTCTATGATT ACGGAATCAG AAGGGTGACA TTTTTAGTTG CACAAAAGCG GGAGTTCCCA 
AAATACTTCA CGTTTAGGGC CAGAGATGAA TTCCATGAAG ACAGAATCTA CCGTAACCTG 
GAGCCTGCTC TGGCCTTCCA GCTGGAGCTG AACCGCATGC GTAACTTTGA CCTTAAGGCT 
GTTCCCTGTG CTCACCACCG CATGCAGCTG TACTTGGGTG CTGCTCGTGT GGAAGAGGGC 
GCAGAGGTCA CTGATTATCG CTTCTTTATC AGAGCCATCA TTCGCCACTC TGACCTCATC 
ACCAAGGAGG CCTCCTTTGA GTATTTGCAG AATGAGGGTG AGAGGCTGTT GCTGGAGGCG 
ATGGATGAGC TGGAGGTGGC CTTTAGTAAC ACACCCACTC GGACTGACTG CAATCACATC 
TTCCTCAATT TTGTACCCAC TGTTATTATG GACCCATACA AGATAGAGGA GTCTGTCCGC 
TCCATGGTGA TGCGATACGG TAGTCGACTG TGGAAGCTGC GAGTTCTCCA GGCTGAGCTG 
AAAATCAACA TCCGCCTCAC CACCACTGGA GATGTTATTC CCATACGCCT CTTTCTCACC 
AATGAGTCTG GATACTATCT GGACATCAGC TTGTATAAAG AGGTCAATGA CCCATCCAGT 
GGACAGATAA TGTTCCAGTC GTATGGAGAC AAACAAGGCC CGCTGAACGG CATGCTCATA 
AACACACCAT ACGTCACAAA GGATTTACTC CAGGCCAAGC GCTTCCAGGC TCAGAGCCTC 
GGCACCACAT ATGTCTATGA CTTCCCTGAG ATGTTTAGAC AGGCTTTGTT TAAGCTTTGG 
GGACCAGGAG ACAGTTATCC TAAAGATGTG TTGATGTGTA ATGAGCTGGT CCTGGATTCT 
CAGGGGAACC TTGTGCAGAT GAACAGACTA CCAGGAGACA ATGAGGTTGG AATGGTAGCT 
TTCCGGATGA GGATGAAGAC TCCAGAGTAC CCAGAGGGCC GAGATATCAT TGTGATCTGT 
AATGATATTA CCCATATGAT TGGCTCTTTT GGGCCTCAGG AGGATCAGCT TTTTATGCGA 
GCATCTGAGC TGGCCAGAGC GGAGGGCATC CCGCGCATCT ACATCTCAGC CAACAGCGGA 
GCTCGAATCG GCCTGGCAGA GGAGATCCGC CACATGTTTC AGGTGGCTTG GATCGACCCT 
GAAGACCCAT ATAAGGGTTT TAAGTACCTG TATCTGACTC CTCAAGACTA CACCCGCATC 
AGTTCCTCCA ATTCTGTCCA TTGTCACCAT GTAGAGGAGG GAGGAGAGTC CAGATACATC 
CTCACAGACA TCATAGGAAA GGAAGAGGGT ATTGGAGTGG AGAATCTGAG GGGCTCGGGC 
ACAATAGCTG GAGAAACTTC TCAGGCTTAT AAAGAAATAA TCACTATCAG CATGGTCACG 
TGTCGGGCTA TAGGAATCGG TGCCTATCTG GTACGTCTAG GACAAAGAGT CATTCAAGTG 
GAAAACTCCC ATATCATCCT GACTGGAGCC GGGGCATTAA ACAAGGTGCT GGGTCGTGAA 
GTCTACACCT CCAATAACCA GCTTGGTGGG GTTCAGATCA TGCACAACAA TGGAGTGACA 
CACTCCACCG TGCCAGATGA CTTTGAAGGA GTGCTCACTA TTCTACAGTG GTTATCCTAC 
ATGCCAAAGA GCAATCAAAG TCCAGTCCCA ATAAAGCCTG CCACTGATCC AGTCGACAGA 
GAGATCGATT TTGTTCCCAC AAAAGCCCCT TATGACCCTC GCTGGCTGCT CTGTGGACGA 
CCCCATCCCA CTGTGAAGGG AGCATGGCAG AGTGGATTCT TTGATCATGG TTCATTTTTG 
GAGATAATGG CTACATGGGC ACAGACAGTG GTGGTGGGCC GAGCCAGGCT TGGTGGGATT 
CCTCTTGGGG TCATTGCTGT GGAGACCCGC ACAGTCGAGC TTGCCATTCC AGCAGATCCT 
GCCAACTTAG ACTCAGAGGC CAAGCTCCTC CAGCAAGCTG GTCAGGTGTG GTTTCCAGAT 
TCTGCATATA AAACGGCTCA GGCTATTGAG GATTTCAATA GAGAGAAACT TCCCCTTATG 
GTGTTTGCCA ACTGGCGAGG ATTTTCTGGA GGCATGAAAG ATATGTACGA CCAGGTGCTC 
AAGTTTGGCT CTTATATCGT GGATGCCCTG CGAGAATTCA GCCAGCCTGT GCTGGTTTAC 
ATTCCTCCTA ATGCTGAGCT GAGAGGAGGA TCATGGGTCG TGATCGACCC TACTATAAAC 
CTTCAGCACA TGGAGCTCTA TGCAGATCGA GAAAGCAGAG GGGGCGTTTT GGAGGCTGAG 
GGCACAGTGG AGATCAAATT CAGAAAAAAA GACCTGCTAA AGACCATGCG AAGGATTGAT 
CCAGTGTATT CTCGTCTGGC TGAGCAGCTA GGCAAACCAG ACTTACCGAG CCAGGAGCGT 
AAAGACCTGG AGGCAAAACT AAAGTCTAGA GAGGAGTTCC TTCTTCCCAT CTACCACCAG 
GTGGCAGTGC AGTTTGTTGA CCTTCATGAC ACTCCAGGAC GGATGCAGGA AAAGGGAGTC 
ATCACGGACA TCCTGGACTG GAAGACTGCT AGGTCATTCT TTTACTGGCG TCTGAGGCGA 
CTGCTGTTGG AGGAAGTGGT AAAGAAGGAG ATCATGCAGG CCAACCAAGA CCTGAGCAAC 
GGACACATAC AGTCCATGCT GCGCCGCTGG TTTGTGGAGA CAGAAGGAGC TGTAAAAGCA 
TATCTGTGGG ACAATAACAA AGTGGTGGTG GAGTGGCTGG AAAACAACCT GTCCCAGCAG 
GACGGAACAC GTTCGGTCAT CAGAGAAAAC ATCAAATGCA TAAAGAGGGA CTACGCTCTC 
AAACACATCC GCAGCTTAGT CCAAGCCAAT CCAGAAGTGA CTATGGACTG CATCATTCAC 
ATGGCACAGA ACATTACGCC TTCACAGAGA GCCAAAGTCT GTCACCTGCT GGCCACCATG 
GACAACTCCA CCACCAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299650.1
CDS	268..7428
Translation	MPSQTPISDCAGEHSPPMDPGLSSTSPRNEGIPPSATSDPTDQSNITVSHIESHRPRFGSGAQAKEKLKFILGASEDNSSDDEPLVKNQTTTHQPKNTETLNAEPTASSEMPPTPVPSTGLRPSMSGPHLLKKGKEHRKMEVHRDFTVASPAEFVTRFGGNRIIDKVLIANNGIAAVKCMRSIRRWSYEMFRNERTIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVEMIVDIAKRIPVQAVWAGWGHASENPKLPELLHKSGICFLGPSSKAMWALGDKVASSIVAQSAGIPTLPWSGTGLSVEWAEEEQRQGRLISVPPELYVQGCVKDVDEGLASAEKIGYPVVIKASEGGGGKGIRKVESSEDFPSLFRQVQAEVPGSPIFIMQLAEHARHLEVQILADQYGNAISLFGRDCSIQRRHQKIIEEAPASIASTITFEQMEQYAVRLAKMVGYVSAGTVEYLFSEDGSFHFLELNPRLQVEHPCTEMIADVNLPAAQLQIAMGIPLYRIKDIRVLFGEAPWGDTTINFESPECMPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSSKNVWGYFSVGATGGLHEFADSQFGHCFSWGENREEAISNMVVAMKELSIRGDFRTTVEYLIKLLETESFRNNDIDTGWLDHLIADKVQAERPDTMLGVVCGALQVADASFRESMSDFLHSLERGQVLPAASLVNTVNVDLIYDGVKYCLKVARQSPTTYVIIMNDSDIEVDVHRLSDGGLLLSYGGSSYTTYMKEEIDRYRVTVGNKTCVFEKERDPTVLRSPSAGKLLQYVVTDGSHVSASQPYAEIEVMKMVMTLHVQHSGCIRFLKRPGTVLEPGCIVALMDLDDPSCIHQVKPNTEPLPAQEPLPMVGERLHQVFHNVLENLVKIMDGYCLPEPYFSQKLKNWVDTLMKTLRDPSLPLLELQEIMTSVAGRIPVTVEKAIRKVMAQYASNITSVLCQFPSQRIANILDSHAATLQRKADREIFFMNTQSIVQLVQRYRSGIRGYMKSVVLDLLKHYLQVEMQFQQAHYDKCVINLREQYKPDMTPVLECIFSHAQVSKKNILVTMLIDQLCGRDPMLADELMVILDELTQLSKMENSKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCPENLKKLILSETSIFDVLPSFFYHNNRVVCMAALEVYVRRAYIAYELNSLQHHQLQDGTCAVDFQFMLPSSHPNREPNATVSQHMPKSTGSSPTLNRLTLPVNDSGEFPAMRRQGSELFLEGALSPPCQRMGAMVAFHSFDHFKRCFDEVICRFVDPLCESSLFFDGCSTFCDGESCKNMKENPIHIINVSIKQADTEDDDALVTAFTAFAHSKKSLLYDYGIRRVTFLVAQKREFPKYFTFRARDEFHEDRIYRNLEPALAFQLELNRMRNFDLKAVPCAHHRMQLYLGAARVEEGAEVTDYRFFIRAIIRHSDLITKEASFEYLQNEGERLLLEAMDELEVAFSNTPTRTDCNHIFLNFVPTVIMDPYKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTTTGDVIPIRLFLTNESGYYLDISLYKEVNDPSSGQIMFQSYGDKQGPLNGMLINTPYVTKDLLQAKRFQAQSLGTTYVYDFPEMFRQALFKLWGPGDSYPKDVLMCNELVLDSQGNLVQMNRLPGDNEVGMVAFRMRMKTPEYPEGRDIIVICNDITHMIGSFGPQEDQLFMRASELARAEGIPRIYISANSGARIGLAEEIRHMFQVAWIDPEDPYKGFKYLYLTPQDYTRISSSNSVHCHHVEEGGESRYILTDIIGKEEGIGVENLRGSGTIAGETSQAYKEIITISMVTCRAIGIGAYLVRLGQRVIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHSTVPDDFEGVLTILQWLSYMPKSNQSPVPIKPATDPVDREIDFVPTKAPYDPRWLLCGRPHPTVKGAWQSGFFDHGSFLEIMATWAQTVVVGRARLGGIPLGVIAVETRTVELAIPADPANLDSEAKLLQQAGQVWFPDSAYKTAQAIEDFNREKLPLMVFANWRGFSGGMKDMYDQVLKFGSYIVDALREFSQPVLVYIPPNAELRGGSWVVIDPTINLQHMELYADRESRGGVLEAEGTVEIKFRKKDLLKTMRRIDPVYSRLAEQLGKPDLPSQERKDLEAKLKSREEFLLPIYHQVAVQFVDLHDTPGRMQEKGVITDILDWKTARSFFYWRLRRLLLEEVVKKEIMQANQDLSNGHIQSMLRRWFVETEGAVKAYLWDNNKVVVEWLENNLSQQDGTRSVIRENIKCIKRDYALKHIRSLVQANPEVTMDCIIHMAQNITPSQRAKVCHLLATMDNSTTS

Target ORF information:

RefSeq Version	XM_009301375.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase beta (acacb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301375.2

ORF Insert Sequence:

ATGCCATCAC AAACACCCAT CTCAGATTGT GCAGGGGAAC ATTCTCCACC AATGGACCCC 
GGATTATCAA GCACTTCACC AAGAAACGAG GGAATTCCTC CATCAGCAAC TAGCGATCCC 
ACTGACCAAT CAAATATCAC AGTTTCCCAT ATTGAATCCC ACAGGCCCAG GTTTGGTTCT 
GGTGCTCAGG CTAAAGAAAA ACTGAAGTTT ATTTTGGGTG CTTCTGAAGA TAATTCCTCT 
GATGATGAGC CTCTGGTTAA GAACCAAACC ACCACACATC AGCCCAAAAA CACAGAGACA 
CTTAATGCTG AACCTACCGC ATCATCAGAA ATGCCGCCAA CACCAGTTCC ATCGACGGGT 
TTAAGGCCTA GCATGTCAGG CCCCCACCTG CTGAAAAAGG GCAAAGAACA CAGGAAGATG 
GAGGTGCATA GAGATTTCAC CGTTGCCTCT CCAGCGGAGT TTGTCACTCG CTTTGGAGGA 
AACCGCATTA TAGATAAGGT GCTGATTGCA AATAATGGCA TTGCTGCAGT TAAGTGCATG 
CGGTCAATTC GCCGCTGGTC CTATGAAATG TTTCGTAATG AGAGAACCAT TCGCTTTGTG 
GTGATGGTCA CACCCGAAGA CTTGAAAGCC AATGCAGAAT ACATTAAAAT GGCTGATCAC 
TATGTGCCAG TACCAGGGGG TCCAAATAAC AACAACTATG CCAATGTTGA GATGATTGTG 
GATATTGCAA AAAGGATACC AGTGCAGGCT GTGTGGGCTG GATGGGGACA TGCATCTGAG 
AACCCCAAAC TACCAGAGCT CCTTCATAAA TCAGGGATAT GTTTTCTAGG ACCGTCCAGT 
AAAGCCATGT GGGCATTAGG AGATAAAGTG GCATCTTCAA TCGTAGCTCA GAGCGCAGGC 
ATTCCCACGT TGCCCTGGAG TGGAACTGGT CTGAGTGTTG AATGGGCAGA AGAAGAGCAG 
AGGCAGGGTC GTTTGATCAG CGTTCCTCCT GAACTATACG TGCAGGGTTG TGTTAAAGAT 
GTGGACGAGG GACTAGCGAG TGCAGAAAAG ATTGGCTACC CTGTGGTCAT CAAAGCATCA 
GAGGGAGGTG GAGGTAAAGG CATAAGAAAA GTTGAAAGTT CTGAAGACTT TCCTAGCCTT 
TTCAGACAGG TGCAGGCTGA GGTGCCAGGT TCACCCATCT TCATAATGCA ACTAGCCGAA 
CACGCACGCC ACCTGGAGGT GCAGATCTTG GCCGACCAAT ATGGCAATGC CATCTCTCTG 
TTTGGCAGAG ACTGTTCTAT CCAGCGCCGT CACCAGAAGA TCATAGAAGA AGCTCCTGCC 
AGCATTGCCT CCACCATCAC TTTTGAGCAA ATGGAGCAGT ATGCAGTTCG TCTGGCTAAA 
ATGGTGGGCT ATGTGAGCGC TGGTACGGTG GAATACCTGT TTTCTGAGGA TGGAAGTTTC 
CACTTCCTGG AGCTGAATCC TCGACTTCAG GTGGAGCATC CTTGTACGGA GATGATTGCT 
GACGTCAACC TTCCTGCAGC ACAGCTACAG ATAGCGATGG GGATTCCTCT TTACAGAATT 
AAGGATATCC GTGTCCTGTT TGGTGAAGCT CCATGGGGAG ACACAACTAT TAACTTTGAA 
TCTCCAGAAT GCATGCCCTG TCCACGGGGA CACGTCATAG CTGCACGCAT CACCAGTGAA 
AACCCGGATG AGGGCTTTAA ACCAAGCTCA GGTACAGTTC AGGAGCTGAA CTTCCGTAGC 
AGTAAGAATG TATGGGGCTA CTTTAGTGTA GGTGCAACTG GAGGCCTACA TGAGTTTGCA 
GACTCTCAGT TTGGACACTG TTTCTCCTGG GGCGAGAACC GAGAAGAGGC CATCTCGAAC 
ATGGTGGTAG CCATGAAGGA GCTGTCAATC AGAGGAGACT TCAGGACAAC AGTGGAGTAC 
CTTATAAAAC TGCTGGAAAC AGAAAGTTTC AGAAACAATG ACATTGATAC TGGTTGGCTG 
GATCACCTTA TTGCTGATAA AGTGCAGGCA GAGAGGCCAG ATACTATGTT AGGTGTGGTA 
TGCGGAGCTC TACAAGTGGC TGATGCAAGT TTTAGAGAAA GCATGTCTGA CTTCCTGCAT 
TCACTGGAAA GGGGTCAGGT GTTGCCGGCT GCTAGTTTGG TCAACACAGT TAATGTTGAT 
CTGATCTATG ATGGCGTCAA ATACTGCCTG AAGGTAGCCC GTCAGTCACC TACGACATAT 
GTTATCATAA TGAATGACTC CGATATAGAA GTTGATGTCC ATAGGCTCAG TGATGGTGGT 
CTACTGCTTT CCTATGGCGG CAGCAGCTAC ACAACCTACA TGAAGGAGGA GATTGATAGG 
TACCGTGTCA CAGTGGGCAA TAAAACGTGC GTGTTTGAGA AGGAGCGAGA CCCTACGGTG 
CTCCGTTCGC CCTCTGCTGG CAAGCTTTTG CAGTATGTAG TCACTGATGG TTCACATGTT 
TCGGCTTCCC AGCCTTATGC TGAAATTGAG GTGATGAAGA TGGTGATGAC CCTGCATGTC 
CAGCATTCTG GTTGTATTCG CTTTTTAAAA AGACCAGGAA CAGTATTGGA GCCTGGCTGT 
ATAGTGGCCC TAATGGACCT GGATGACCCC AGCTGTATTC ATCAGGTGAA ACCAAACACA 
GAGCCATTAC CAGCTCAGGA GCCATTACCC ATGGTAGGAG AGAGGCTTCA CCAGGTGTTC 
CACAATGTTC TGGAGAACCT GGTGAAAATT ATGGATGGAT ATTGTCTGCC AGAGCCATAT 
TTTAGTCAGA AATTGAAGAA CTGGGTCGAC ACGCTGATGA AGACGCTTCG GGATCCGTCT 
CTGCCTCTAC TTGAACTGCA GGAGATCATG ACCAGCGTGG CAGGGCGAAT TCCAGTTACT 
GTGGAGAAGG CAATTCGAAA GGTCATGGCA CAGTACGCCA GTAACATTAC CTCTGTGCTC 
TGTCAGTTCC CCAGCCAAAG GATAGCAAAT ATACTTGATA GCCATGCTGC CACACTTCAA 
AGGAAAGCAG ACCGGGAAAT TTTCTTCATG AACACCCAGA GCATTGTGCA ATTGGTGCAA 
AGATACCGTA GCGGCATTAG GGGCTACATG AAATCTGTTG TTCTGGATCT TTTGAAACAC 
TACCTTCAGG TGGAGATGCA ATTTCAGCAA GCTCACTATG ACAAGTGTGT GATCAATCTC 
AGAGAGCAGT ATAAGCCGGA CATGACCCCA GTGCTGGAGT GCATCTTTTC TCATGCTCAG 
GTGTCCAAGA AGAACATCCT CGTCACCATG CTCATAGACC AGCTGTGTGG CCGGGACCCA 
ATGCTTGCTG ATGAGCTCAT GGTCATTCTG GATGAGCTTA CACAACTCAG TAAAATGGAA 
AACTCTAAAG TGGCACTTAG GGCCAGACAG GTGTTGATCG CCTCTCATTT GCCCTCATAT 
GAACTCAGGC ACAACCAGGT GGAATCCATC TTTTTATCAG CTATTGATAT GTATGGGCAT 
CAGTTTTGTC CTGAAAACCT CAAGAAACTA ATCCTATCTG AGACCTCCAT CTTTGACGTC 
TTGCCCAGTT TTTTCTACCA CAACAACCGT GTGGTTTGCA TGGCTGCATT AGAGGTTTAT 
GTACGGAGGG CTTATATAGC TTACGAGCTA AACAGTCTTC AGCACCATCA GCTGCAGGAT 
GGGACATGTG CGGTGGATTT CCAGTTCATG TTACCATCAT CCCACCCAAA CAGAGAGCCT 
AACGCCACAG TGAGCCAGCA CATGCCCAAA TCCACAGGGA GCAGTCCTAC TTTGAACAGA 
CTGACCCTTC CTGTCAACGA TTCGGGAGAG TTTCCGGCAA TGCGCCGTCA AGGCAGTGAG 
CTCTTTCTAG AGGGGGCGCT TTCACCCCCC TGCCAGAGGA TGGGAGCCAT GGTTGCCTTC 
CATAGCTTTG ACCACTTCAA AAGGTGCTTT GATGAAGTGA TCTGTCGCTT TGTGGATCCG 
CTGTGTGAAA GTTCTCTGTT TTTTGATGGC TGTTCCACCT TCTGTGATGG GGAGAGCTGT 
AAAAACATGA AGGAAAACCC CATTCACATC ATAAATGTGT CAATTAAACA AGCAGACACA 
GAAGATGATG ATGCTTTGGT CACAGCTTTC ACTGCCTTTG CTCACTCCAA AAAATCGCTA 
CTCTATGATT ACGGAATCAG AAGGGTGACA TTTTTAGTTG CACAAAAGCG GGAGTTCCCA 
AAATACTTCA CGTTTAGGGC CAGAGATGAA TTCCATGAAG ACAGAATCTA CCGTAACCTG 
GAGCCTGCTC TGGCCTTCCA GCTGGAGCTG AACCGCATGC GTAACTTTGA CCTTAAGGCT 
GTTCCCTGTG CTCACCACCG CATGCAGCTG TACTTGGGTG CTGCTCGTGT GGAAGAGGGC 
GCAGAGGTCA CTGATTATCG CTTCTTTATC AGAGCCATCA TTCGCCACTC TGACCTCATC 
ACCAAGGAGG CCTCCTTTGA GTATTTGCAG AATGAGGGTG AGAGGCTGTT GCTGGAGGCG 
ATGGATGAGC TGGAGGTGGC CTTTAGTAAC ACACCCACTC GGACTGACTG CAATCACATC 
TTCCTCAATT TTGTACCCAC TGTTATTATG GACCCATACA AGATAGAGGA GTCTGTCCGC 
TCCATGGTGA TGCGATACGG TAGTCGACTG TGGAAGCTGC GAGTTCTCCA GGCTGAGCTG 
AAAATCAACA TCCGCCTCAC CACCACTGGA GATGTTATTC CCATACGCCT CTTTCTCACC 
AATGAGTCTG GATACTATCT GGACATCAGC TTGTATAAAG AGGTCAATGA CCCATCCAGT 
GGACAGATAA TGTTCCAGTC GTATGGAGAC AAACAAGGCC CGCTGAACGG CATGCTCATA 
AACACACCAT ACGTCACAAA GGATTTACTC CAGGCCAAGC GCTTCCAGGC TCAGAGCCTC 
GGCACCACAT ATGTCTATGA CTTCCCTGAG ATGTTTAGAC AGGCTTTGTT TAAGCTTTGG 
GGACCAGGAG ACAGTTATCC TAAAGATGTG TTGATGTGTA ATGAGCTGGT CCTGGATTCT 
CAGGGGAACC TTGTGCAGAT GAACAGACTA CCAGGAGACA ATGAGGTTGG AATGGTAGCT 
TTCCGGATGA GGATGAAGAC TCCAGAGTAC CCAGAGGGCC GAGATATCAT TGTGATCTGT 
AATGATATTA CCCATATGAT TGGCTCTTTT GGGCCTCAGG AGGATCAGCT TTTTATGCGA 
GCATCTGAGC TGGCCAGAGC GGAGGGCATC CCGCGCATCT ACATCTCAGC CAACAGCGGA 
GCTCGAATCG GCCTGGCAGA GGAGATCCGC CACATGTTTC AGGTGGCTTG GATCGACCCT 
GAAGACCCAT ATAAGGGTTT TAAGTACCTG TATCTGACTC CTCAAGACTA CACCCGCATC 
AGTTCCTCCA ATTCTGTCCA TTGTCACCAT GTAGAGGAGG GAGGAGAGTC CAGATACATC 
CTCACAGACA TCATAGGAAA GGAAGAGGGT ATTGGAGTGG AGAATCTGAG GGGCTCGGGC 
ACAATAGCTG GAGAAACTTC TCAGGCTTAT AAAGAAATAA TCACTATCAG CATGGTCACG 
TGTCGGGCTA TAGGAATCGG TGCCTATCTG GTACGTCTAG GACAAAGAGT CATTCAAGTG 
GAAAACTCCC ATATCATCCT GACTGGAGCC GGGGCATTAA ACAAGGTGCT GGGTCGTGAA 
GTCTACACCT CCAATAACCA GCTTGGTGGG GTTCAGATCA TGCACAACAA TGGAGTGACA 
CACTCCACCG TGCCAGATGA CTTTGAAGGA GTGCTCACTA TTCTACAGTG GTTATCCTAC 
ATGCCAAAGA GCAATCAAAG TCCAGTCCCA ATAAAGCCTG CCACTGATCC AGTCGACAGA 
GAGATCGATT TTGTTCCCAC AAAAGCCCCT TATGACCCTC GCTGGCTGCT CTGTGGACGA 
CCCCATCCCA CTGTGAAGGG AGCATGGCAG AGTGGATTCT TTGATCATGG TTCATTTTTG 
GAGATAATGG CTACATGGGC ACAGACAGTG GTGGTGGGCC GAGCCAGGCT TGGTGGGATT 
CCTCTTGGGG TCATTGCTGT GGAGACCCGC ACAGTCGAGC TTGCCATTCC AGCAGATCCT 
GCCAACTTAG ACTCAGAGGC CAAGCTCCTC CAGCAAGCTG GTCAGGTGTG GTTTCCAGAT 
TCTGCATATA AAACGGCTCA GGCTATTGAG GATTTCAATA GAGAGAAACT TCCCCTTATG 
GTGTTTGCCA ACTGGCGAGG ATTTTCTGGA GGCATGAAAG ATATGTACGA CCAGGTGCTC 
AAGTTTGGCT CTTATATCGT GGATGCCCTG CGAGAATTCA GCCAGCCTGT GCTGGTTTAC 
ATTCCTCCTA ATGCTGAGCT GAGAGGAGGA TCATGGGTCG TGATCGACCC TACTATAAAC 
CTTCAGCACA TGGAGCTCTA TGCAGATCGA GAAAGCAGAG GGGGCGTTTT GGAGGCTGAG 
GGCACAGTGG AGATCAAATT CAGAAAAAAA GACCTGCTAA AGACCATGCG AAGGATTGAT 
CCAGTGTATT CTCGTCTGGC TGAGCAGCTA GGCAAACCAG ACTTACCGAG CCAGGAGCGT 
AAAGACCTGG AGGCAAAACT AAAGTCTAGA GAGGAGTTCC TTCTTCCCAT CTACCACCAG 
GTGGCAGTGC AGTTTGTTGA CCTTCATGAC ACTCCAGGAC GGATGCAGGA AAAGGGAGTC 
ATCACGGACA TCCTGGACTG GAAGACTGCT AGGTCATTCT TTTACTGGCG TCTGAGGCGA 
CTGCTGTTGG AGGAAGTGGT AAAGAAGGAG ATCATGCAGG CCAACCAAGA CCTGAGCAAC 
GGACACATAC AGTCCATGCT GCGCCGCTGG TTTGTGGAGA CAGAAGGAGC TGTAAAAGCA 
TATCTGTGGG ACAATAACAA AGTGGTGGTG GAGTGGCTGG AAAACAACCT GTCCCAGCAG 
GACGGAACAC GTTCGGTCAT CAGAGAAAAC ATCAAATGCA TAAAGAGGGA CTACGCTCTC 
AAACACATCC GCAGCTTAGT CCAAGCCAAT CCAGAAGTGA CTATGGACTG CATCATTCAC 
ATGGCACAGA ACATTACGCC TTCACAGAGA GCCAAAGTCT GTCACCTGCT GGCCACCATG 
GACAACTCCA CCACCAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acacb ( XM_021476432.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332107.1 Danio rerio acetyl-CoA carboxylase beta (acacb), transcript variant X1, mRNA.

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CloneID	ODa42328
Clone ID Related Accession (Same CDS sequence)	XM_009301375.2 , XM_021476432.1
Accession Version	XM_021476432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	acetyl-CoA carboxylase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCATCAC AAACACCCAT CTCAGATTGT GCAGGGGAAC ATTCTCCACC AATGGACCCC 
GGATTATCAA GCACTTCACC AAGAAACGAG GGAATTCCTC CATCAGCAAC TAGCGATCCC 
ACTGACCAAT CAAATATCAC AGTTTCCCAT ATTGAATCCC ACAGGCCCAG GTTTGGTTCT 
GGTGCTCAGG CTAAAGAAAA ACTGAAGTTT ATTTTGGGTG CTTCTGAAGA TAATTCCTCT 
GATGATGAGC CTCTGGTTAA GAACCAAACC ACCACACATC AGCCCAAAAA CACAGAGACA 
CTTAATGCTG AACCTACCGC ATCATCAGAA ATGCCGCCAA CACCAGTTCC ATCGACGGGT 
TTAAGGCCTA GCATGTCAGG CCCCCACCTG CTGAAAAAGG GCAAAGAACA CAGGAAGATG 
GAGGTGCATA GAGATTTCAC CGTTGCCTCT CCAGCGGAGT TTGTCACTCG CTTTGGAGGA 
AACCGCATTA TAGATAAGGT GCTGATTGCA AATAATGGCA TTGCTGCAGT TAAGTGCATG 
CGGTCAATTC GCCGCTGGTC CTATGAAATG TTTCGTAATG AGAGAACCAT TCGCTTTGTG 
GTGATGGTCA CACCCGAAGA CTTGAAAGCC AATGCAGAAT ACATTAAAAT GGCTGATCAC 
TATGTGCCAG TACCAGGGGG TCCAAATAAC AACAACTATG CCAATGTTGA GATGATTGTG 
GATATTGCAA AAAGGATACC AGTGCAGGCT GTGTGGGCTG GATGGGGACA TGCATCTGAG 
AACCCCAAAC TACCAGAGCT CCTTCATAAA TCAGGGATAT GTTTTCTAGG ACCGTCCAGT 
AAAGCCATGT GGGCATTAGG AGATAAAGTG GCATCTTCAA TCGTAGCTCA GAGCGCAGGC 
ATTCCCACGT TGCCCTGGAG TGGAACTGGT CTGAGTGTTG AATGGGCAGA AGAAGAGCAG 
AGGCAGGGTC GTTTGATCAG CGTTCCTCCT GAACTATACG TGCAGGGTTG TGTTAAAGAT 
GTGGACGAGG GACTAGCGAG TGCAGAAAAG ATTGGCTACC CTGTGGTCAT CAAAGCATCA 
GAGGGAGGTG GAGGTAAAGG CATAAGAAAA GTTGAAAGTT CTGAAGACTT TCCTAGCCTT 
TTCAGACAGG TGCAGGCTGA GGTGCCAGGT TCACCCATCT TCATAATGCA ACTAGCCGAA 
CACGCACGCC ACCTGGAGGT GCAGATCTTG GCCGACCAAT ATGGCAATGC CATCTCTCTG 
TTTGGCAGAG ACTGTTCTAT CCAGCGCCGT CACCAGAAGA TCATAGAAGA AGCTCCTGCC 
AGCATTGCCT CCACCATCAC TTTTGAGCAA ATGGAGCAGT ATGCAGTTCG TCTGGCTAAA 
ATGGTGGGCT ATGTGAGCGC TGGTACGGTG GAATACCTGT TTTCTGAGGA TGGAAGTTTC 
CACTTCCTGG AGCTGAATCC TCGACTTCAG GTGGAGCATC CTTGTACGGA GATGATTGCT 
GACGTCAACC TTCCTGCAGC ACAGCTACAG ATAGCGATGG GGATTCCTCT TTACAGAATT 
AAGGATATCC GTGTCCTGTT TGGTGAAGCT CCATGGGGAG ACACAACTAT TAACTTTGAA 
TCTCCAGAAT GCATGCCCTG TCCACGGGGA CACGTCATAG CTGCACGCAT CACCAGTGAA 
AACCCGGATG AGGGCTTTAA ACCAAGCTCA GGTACAGTTC AGGAGCTGAA CTTCCGTAGC 
AGTAAGAATG TATGGGGCTA CTTTAGTGTA GGTGCAACTG GAGGCCTACA TGAGTTTGCA 
GACTCTCAGT TTGGACACTG TTTCTCCTGG GGCGAGAACC GAGAAGAGGC CATCTCGAAC 
ATGGTGGTAG CCATGAAGGA GCTGTCAATC AGAGGAGACT TCAGGACAAC AGTGGAGTAC 
CTTATAAAAC TGCTGGAAAC AGAAAGTTTC AGAAACAATG ACATTGATAC TGGTTGGCTG 
GATCACCTTA TTGCTGATAA AGTGCAGGCA GAGAGGCCAG ATACTATGTT AGGTGTGGTA 
TGCGGAGCTC TACAAGTGGC TGATGCAAGT TTTAGAGAAA GCATGTCTGA CTTCCTGCAT 
TCACTGGAAA GGGGTCAGGT GTTGCCGGCT GCTAGTTTGG TCAACACAGT TAATGTTGAT 
CTGATCTATG ATGGCGTCAA ATACTGCCTG AAGGTAGCCC GTCAGTCACC TACGACATAT 
GTTATCATAA TGAATGACTC CGATATAGAA GTTGATGTCC ATAGGCTCAG TGATGGTGGT 
CTACTGCTTT CCTATGGCGG CAGCAGCTAC ACAACCTACA TGAAGGAGGA GATTGATAGG 
TACCGTGTCA CAGTGGGCAA TAAAACGTGC GTGTTTGAGA AGGAGCGAGA CCCTACGGTG 
CTCCGTTCGC CCTCTGCTGG CAAGCTTTTG CAGTATGTAG TCACTGATGG TTCACATGTT 
TCGGCTTCCC AGCCTTATGC TGAAATTGAG GTGATGAAGA TGGTGATGAC CCTGCATGTC 
CAGCATTCTG GTTGTATTCG CTTTTTAAAA AGACCAGGAA CAGTATTGGA GCCTGGCTGT 
ATAGTGGCCC TAATGGACCT GGATGACCCC AGCTGTATTC ATCAGGTGAA ACCAAACACA 
GAGCCATTAC CAGCTCAGGA GCCATTACCC ATGGTAGGAG AGAGGCTTCA CCAGGTGTTC 
CACAATGTTC TGGAGAACCT GGTGAAAATT ATGGATGGAT ATTGTCTGCC AGAGCCATAT 
TTTAGTCAGA AATTGAAGAA CTGGGTCGAC ACGCTGATGA AGACGCTTCG GGATCCGTCT 
CTGCCTCTAC TTGAACTGCA GGAGATCATG ACCAGCGTGG CAGGGCGAAT TCCAGTTACT 
GTGGAGAAGG CAATTCGAAA GGTCATGGCA CAGTACGCCA GTAACATTAC CTCTGTGCTC 
TGTCAGTTCC CCAGCCAAAG GATAGCAAAT ATACTTGATA GCCATGCTGC CACACTTCAA 
AGGAAAGCAG ACCGGGAAAT TTTCTTCATG AACACCCAGA GCATTGTGCA ATTGGTGCAA 
AGATACCGTA GCGGCATTAG GGGCTACATG AAATCTGTTG TTCTGGATCT TTTGAAACAC 
TACCTTCAGG TGGAGATGCA ATTTCAGCAA GCTCACTATG ACAAGTGTGT GATCAATCTC 
AGAGAGCAGT ATAAGCCGGA CATGACCCCA GTGCTGGAGT GCATCTTTTC TCATGCTCAG 
GTGTCCAAGA AGAACATCCT CGTCACCATG CTCATAGACC AGCTGTGTGG CCGGGACCCA 
ATGCTTGCTG ATGAGCTCAT GGTCATTCTG GATGAGCTTA CACAACTCAG TAAAATGGAA 
AACTCTAAAG TGGCACTTAG GGCCAGACAG GTGTTGATCG CCTCTCATTT GCCCTCATAT 
GAACTCAGGC ACAACCAGGT GGAATCCATC TTTTTATCAG CTATTGATAT GTATGGGCAT 
CAGTTTTGTC CTGAAAACCT CAAGAAACTA ATCCTATCTG AGACCTCCAT CTTTGACGTC 
TTGCCCAGTT TTTTCTACCA CAACAACCGT GTGGTTTGCA TGGCTGCATT AGAGGTTTAT 
GTACGGAGGG CTTATATAGC TTACGAGCTA AACAGTCTTC AGCACCATCA GCTGCAGGAT 
GGGACATGTG CGGTGGATTT CCAGTTCATG TTACCATCAT CCCACCCAAA CAGAGAGCCT 
AACGCCACAG TGAGCCAGCA CATGCCCAAA TCCACAGGGA GCAGTCCTAC TTTGAACAGA 
CTGACCCTTC CTGTCAACGA TTCGGGAGAG TTTCCGGCAA TGCGCCGTCA AGGCAGTGAG 
CTCTTTCTAG AGGGGGCGCT TTCACCCCCC TGCCAGAGGA TGGGAGCCAT GGTTGCCTTC 
CATAGCTTTG ACCACTTCAA AAGGTGCTTT GATGAAGTGA TCTGTCGCTT TGTGGATCCG 
CTGTGTGAAA GTTCTCTGTT TTTTGATGGC TGTTCCACCT TCTGTGATGG GGAGAGCTGT 
AAAAACATGA AGGAAAACCC CATTCACATC ATAAATGTGT CAATTAAACA AGCAGACACA 
GAAGATGATG ATGCTTTGGT CACAGCTTTC ACTGCCTTTG CTCACTCCAA AAAATCGCTA 
CTCTATGATT ACGGAATCAG AAGGGTGACA TTTTTAGTTG CACAAAAGCG GGAGTTCCCA 
AAATACTTCA CGTTTAGGGC CAGAGATGAA TTCCATGAAG ACAGAATCTA CCGTAACCTG 
GAGCCTGCTC TGGCCTTCCA GCTGGAGCTG AACCGCATGC GTAACTTTGA CCTTAAGGCT 
GTTCCCTGTG CTCACCACCG CATGCAGCTG TACTTGGGTG CTGCTCGTGT GGAAGAGGGC 
GCAGAGGTCA CTGATTATCG CTTCTTTATC AGAGCCATCA TTCGCCACTC TGACCTCATC 
ACCAAGGAGG CCTCCTTTGA GTATTTGCAG AATGAGGGTG AGAGGCTGTT GCTGGAGGCG 
ATGGATGAGC TGGAGGTGGC CTTTAGTAAC ACACCCACTC GGACTGACTG CAATCACATC 
TTCCTCAATT TTGTACCCAC TGTTATTATG GACCCATACA AGATAGAGGA GTCTGTCCGC 
TCCATGGTGA TGCGATACGG TAGTCGACTG TGGAAGCTGC GAGTTCTCCA GGCTGAGCTG 
AAAATCAACA TCCGCCTCAC CACCACTGGA GATGTTATTC CCATACGCCT CTTTCTCACC 
AATGAGTCTG GATACTATCT GGACATCAGC TTGTATAAAG AGGTCAATGA CCCATCCAGT 
GGACAGATAA TGTTCCAGTC GTATGGAGAC AAACAAGGCC CGCTGAACGG CATGCTCATA 
AACACACCAT ACGTCACAAA GGATTTACTC CAGGCCAAGC GCTTCCAGGC TCAGAGCCTC 
GGCACCACAT ATGTCTATGA CTTCCCTGAG ATGTTTAGAC AGGCTTTGTT TAAGCTTTGG 
GGACCAGGAG ACAGTTATCC TAAAGATGTG TTGATGTGTA ATGAGCTGGT CCTGGATTCT 
CAGGGGAACC TTGTGCAGAT GAACAGACTA CCAGGAGACA ATGAGGTTGG AATGGTAGCT 
TTCCGGATGA GGATGAAGAC TCCAGAGTAC CCAGAGGGCC GAGATATCAT TGTGATCTGT 
AATGATATTA CCCATATGAT TGGCTCTTTT GGGCCTCAGG AGGATCAGCT TTTTATGCGA 
GCATCTGAGC TGGCCAGAGC GGAGGGCATC CCGCGCATCT ACATCTCAGC CAACAGCGGA 
GCTCGAATCG GCCTGGCAGA GGAGATCCGC CACATGTTTC AGGTGGCTTG GATCGACCCT 
GAAGACCCAT ATAAGGGTTT TAAGTACCTG TATCTGACTC CTCAAGACTA CACCCGCATC 
AGTTCCTCCA ATTCTGTCCA TTGTCACCAT GTAGAGGAGG GAGGAGAGTC CAGATACATC 
CTCACAGACA TCATAGGAAA GGAAGAGGGT ATTGGAGTGG AGAATCTGAG GGGCTCGGGC 
ACAATAGCTG GAGAAACTTC TCAGGCTTAT AAAGAAATAA TCACTATCAG CATGGTCACG 
TGTCGGGCTA TAGGAATCGG TGCCTATCTG GTACGTCTAG GACAAAGAGT CATTCAAGTG 
GAAAACTCCC ATATCATCCT GACTGGAGCC GGGGCATTAA ACAAGGTGCT GGGTCGTGAA 
GTCTACACCT CCAATAACCA GCTTGGTGGG GTTCAGATCA TGCACAACAA TGGAGTGACA 
CACTCCACCG TGCCAGATGA CTTTGAAGGA GTGCTCACTA TTCTACAGTG GTTATCCTAC 
ATGCCAAAGA GCAATCAAAG TCCAGTCCCA ATAAAGCCTG CCACTGATCC AGTCGACAGA 
GAGATCGATT TTGTTCCCAC AAAAGCCCCT TATGACCCTC GCTGGCTGCT CTGTGGACGA 
CCCCATCCCA CTGTGAAGGG AGCATGGCAG AGTGGATTCT TTGATCATGG TTCATTTTTG 
GAGATAATGG CTACATGGGC ACAGACAGTG GTGGTGGGCC GAGCCAGGCT TGGTGGGATT 
CCTCTTGGGG TCATTGCTGT GGAGACCCGC ACAGTCGAGC TTGCCATTCC AGCAGATCCT 
GCCAACTTAG ACTCAGAGGC CAAGCTCCTC CAGCAAGCTG GTCAGGTGTG GTTTCCAGAT 
TCTGCATATA AAACGGCTCA GGCTATTGAG GATTTCAATA GAGAGAAACT TCCCCTTATG 
GTGTTTGCCA ACTGGCGAGG ATTTTCTGGA GGCATGAAAG ATATGTACGA CCAGGTGCTC 
AAGTTTGGCT CTTATATCGT GGATGCCCTG CGAGAATTCA GCCAGCCTGT GCTGGTTTAC 
ATTCCTCCTA ATGCTGAGCT GAGAGGAGGA TCATGGGTCG TGATCGACCC TACTATAAAC 
CTTCAGCACA TGGAGCTCTA TGCAGATCGA GAAAGCAGAG GGGGCGTTTT GGAGGCTGAG 
GGCACAGTGG AGATCAAATT CAGAAAAAAA GACCTGCTAA AGACCATGCG AAGGATTGAT 
CCAGTGTATT CTCGTCTGGC TGAGCAGCTA GGCAAACCAG ACTTACCGAG CCAGGAGCGT 
AAAGACCTGG AGGCAAAACT AAAGTCTAGA GAGGAGTTCC TTCTTCCCAT CTACCACCAG 
GTGGCAGTGC AGTTTGTTGA CCTTCATGAC ACTCCAGGAC GGATGCAGGA AAAGGGAGTC 
ATCACGGACA TCCTGGACTG GAAGACTGCT AGGTCATTCT TTTACTGGCG TCTGAGGCGA 
CTGCTGTTGG AGGAAGTGGT AAAGAAGGAG ATCATGCAGG CCAACCAAGA CCTGAGCAAC 
GGACACATAC AGTCCATGCT GCGCCGCTGG TTTGTGGAGA CAGAAGGAGC TGTAAAAGCA 
TATCTGTGGG ACAATAACAA AGTGGTGGTG GAGTGGCTGG AAAACAACCT GTCCCAGCAG 
GACGGAACAC GTTCGGTCAT CAGAGAAAAC ATCAAATGCA TAAAGAGGGA CTACGCTCTC 
AAACACATCC GCAGCTTAGT CCAAGCCAAT CCAGAAGTGA CTATGGACTG CATCATTCAC 
ATGGCACAGA ACATTACGCC TTCACAGAGA GCCAAAGTCT GTCACCTGCT GGCCACCATG 
GACAACTCCA CCACCAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332107.1
CDS	225..7385
Translation	MPSQTPISDCAGEHSPPMDPGLSSTSPRNEGIPPSATSDPTDQSNITVSHIESHRPRFGSGAQAKEKLKFILGASEDNSSDDEPLVKNQTTTHQPKNTETLNAEPTASSEMPPTPVPSTGLRPSMSGPHLLKKGKEHRKMEVHRDFTVASPAEFVTRFGGNRIIDKVLIANNGIAAVKCMRSIRRWSYEMFRNERTIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVEMIVDIAKRIPVQAVWAGWGHASENPKLPELLHKSGICFLGPSSKAMWALGDKVASSIVAQSAGIPTLPWSGTGLSVEWAEEEQRQGRLISVPPELYVQGCVKDVDEGLASAEKIGYPVVIKASEGGGGKGIRKVESSEDFPSLFRQVQAEVPGSPIFIMQLAEHARHLEVQILADQYGNAISLFGRDCSIQRRHQKIIEEAPASIASTITFEQMEQYAVRLAKMVGYVSAGTVEYLFSEDGSFHFLELNPRLQVEHPCTEMIADVNLPAAQLQIAMGIPLYRIKDIRVLFGEAPWGDTTINFESPECMPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSSKNVWGYFSVGATGGLHEFADSQFGHCFSWGENREEAISNMVVAMKELSIRGDFRTTVEYLIKLLETESFRNNDIDTGWLDHLIADKVQAERPDTMLGVVCGALQVADASFRESMSDFLHSLERGQVLPAASLVNTVNVDLIYDGVKYCLKVARQSPTTYVIIMNDSDIEVDVHRLSDGGLLLSYGGSSYTTYMKEEIDRYRVTVGNKTCVFEKERDPTVLRSPSAGKLLQYVVTDGSHVSASQPYAEIEVMKMVMTLHVQHSGCIRFLKRPGTVLEPGCIVALMDLDDPSCIHQVKPNTEPLPAQEPLPMVGERLHQVFHNVLENLVKIMDGYCLPEPYFSQKLKNWVDTLMKTLRDPSLPLLELQEIMTSVAGRIPVTVEKAIRKVMAQYASNITSVLCQFPSQRIANILDSHAATLQRKADREIFFMNTQSIVQLVQRYRSGIRGYMKSVVLDLLKHYLQVEMQFQQAHYDKCVINLREQYKPDMTPVLECIFSHAQVSKKNILVTMLIDQLCGRDPMLADELMVILDELTQLSKMENSKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCPENLKKLILSETSIFDVLPSFFYHNNRVVCMAALEVYVRRAYIAYELNSLQHHQLQDGTCAVDFQFMLPSSHPNREPNATVSQHMPKSTGSSPTLNRLTLPVNDSGEFPAMRRQGSELFLEGALSPPCQRMGAMVAFHSFDHFKRCFDEVICRFVDPLCESSLFFDGCSTFCDGESCKNMKENPIHIINVSIKQADTEDDDALVTAFTAFAHSKKSLLYDYGIRRVTFLVAQKREFPKYFTFRARDEFHEDRIYRNLEPALAFQLELNRMRNFDLKAVPCAHHRMQLYLGAARVEEGAEVTDYRFFIRAIIRHSDLITKEASFEYLQNEGERLLLEAMDELEVAFSNTPTRTDCNHIFLNFVPTVIMDPYKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTTTGDVIPIRLFLTNESGYYLDISLYKEVNDPSSGQIMFQSYGDKQGPLNGMLINTPYVTKDLLQAKRFQAQSLGTTYVYDFPEMFRQALFKLWGPGDSYPKDVLMCNELVLDSQGNLVQMNRLPGDNEVGMVAFRMRMKTPEYPEGRDIIVICNDITHMIGSFGPQEDQLFMRASELARAEGIPRIYISANSGARIGLAEEIRHMFQVAWIDPEDPYKGFKYLYLTPQDYTRISSSNSVHCHHVEEGGESRYILTDIIGKEEGIGVENLRGSGTIAGETSQAYKEIITISMVTCRAIGIGAYLVRLGQRVIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHSTVPDDFEGVLTILQWLSYMPKSNQSPVPIKPATDPVDREIDFVPTKAPYDPRWLLCGRPHPTVKGAWQSGFFDHGSFLEIMATWAQTVVVGRARLGGIPLGVIAVETRTVELAIPADPANLDSEAKLLQQAGQVWFPDSAYKTAQAIEDFNREKLPLMVFANWRGFSGGMKDMYDQVLKFGSYIVDALREFSQPVLVYIPPNAELRGGSWVVIDPTINLQHMELYADRESRGGVLEAEGTVEIKFRKKDLLKTMRRIDPVYSRLAEQLGKPDLPSQERKDLEAKLKSREEFLLPIYHQVAVQFVDLHDTPGRMQEKGVITDILDWKTARSFFYWRLRRLLLEEVVKKEIMQANQDLSNGHIQSMLRRWFVETEGAVKAYLWDNNKVVVEWLENNLSQQDGTRSVIRENIKCIKRDYALKHIRSLVQANPEVTMDCIIHMAQNITPSQRAKVCHLLATMDNSTTS

Target ORF information:

RefSeq Version	XM_021476432.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase beta (acacb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476432.1

ORF Insert Sequence:

ATGCCATCAC AAACACCCAT CTCAGATTGT GCAGGGGAAC ATTCTCCACC AATGGACCCC 
GGATTATCAA GCACTTCACC AAGAAACGAG GGAATTCCTC CATCAGCAAC TAGCGATCCC 
ACTGACCAAT CAAATATCAC AGTTTCCCAT ATTGAATCCC ACAGGCCCAG GTTTGGTTCT 
GGTGCTCAGG CTAAAGAAAA ACTGAAGTTT ATTTTGGGTG CTTCTGAAGA TAATTCCTCT 
GATGATGAGC CTCTGGTTAA GAACCAAACC ACCACACATC AGCCCAAAAA CACAGAGACA 
CTTAATGCTG AACCTACCGC ATCATCAGAA ATGCCGCCAA CACCAGTTCC ATCGACGGGT 
TTAAGGCCTA GCATGTCAGG CCCCCACCTG CTGAAAAAGG GCAAAGAACA CAGGAAGATG 
GAGGTGCATA GAGATTTCAC CGTTGCCTCT CCAGCGGAGT TTGTCACTCG CTTTGGAGGA 
AACCGCATTA TAGATAAGGT GCTGATTGCA AATAATGGCA TTGCTGCAGT TAAGTGCATG 
CGGTCAATTC GCCGCTGGTC CTATGAAATG TTTCGTAATG AGAGAACCAT TCGCTTTGTG 
GTGATGGTCA CACCCGAAGA CTTGAAAGCC AATGCAGAAT ACATTAAAAT GGCTGATCAC 
TATGTGCCAG TACCAGGGGG TCCAAATAAC AACAACTATG CCAATGTTGA GATGATTGTG 
GATATTGCAA AAAGGATACC AGTGCAGGCT GTGTGGGCTG GATGGGGACA TGCATCTGAG 
AACCCCAAAC TACCAGAGCT CCTTCATAAA TCAGGGATAT GTTTTCTAGG ACCGTCCAGT 
AAAGCCATGT GGGCATTAGG AGATAAAGTG GCATCTTCAA TCGTAGCTCA GAGCGCAGGC 
ATTCCCACGT TGCCCTGGAG TGGAACTGGT CTGAGTGTTG AATGGGCAGA AGAAGAGCAG 
AGGCAGGGTC GTTTGATCAG CGTTCCTCCT GAACTATACG TGCAGGGTTG TGTTAAAGAT 
GTGGACGAGG GACTAGCGAG TGCAGAAAAG ATTGGCTACC CTGTGGTCAT CAAAGCATCA 
GAGGGAGGTG GAGGTAAAGG CATAAGAAAA GTTGAAAGTT CTGAAGACTT TCCTAGCCTT 
TTCAGACAGG TGCAGGCTGA GGTGCCAGGT TCACCCATCT TCATAATGCA ACTAGCCGAA 
CACGCACGCC ACCTGGAGGT GCAGATCTTG GCCGACCAAT ATGGCAATGC CATCTCTCTG 
TTTGGCAGAG ACTGTTCTAT CCAGCGCCGT CACCAGAAGA TCATAGAAGA AGCTCCTGCC 
AGCATTGCCT CCACCATCAC TTTTGAGCAA ATGGAGCAGT ATGCAGTTCG TCTGGCTAAA 
ATGGTGGGCT ATGTGAGCGC TGGTACGGTG GAATACCTGT TTTCTGAGGA TGGAAGTTTC 
CACTTCCTGG AGCTGAATCC TCGACTTCAG GTGGAGCATC CTTGTACGGA GATGATTGCT 
GACGTCAACC TTCCTGCAGC ACAGCTACAG ATAGCGATGG GGATTCCTCT TTACAGAATT 
AAGGATATCC GTGTCCTGTT TGGTGAAGCT CCATGGGGAG ACACAACTAT TAACTTTGAA 
TCTCCAGAAT GCATGCCCTG TCCACGGGGA CACGTCATAG CTGCACGCAT CACCAGTGAA 
AACCCGGATG AGGGCTTTAA ACCAAGCTCA GGTACAGTTC AGGAGCTGAA CTTCCGTAGC 
AGTAAGAATG TATGGGGCTA CTTTAGTGTA GGTGCAACTG GAGGCCTACA TGAGTTTGCA 
GACTCTCAGT TTGGACACTG TTTCTCCTGG GGCGAGAACC GAGAAGAGGC CATCTCGAAC 
ATGGTGGTAG CCATGAAGGA GCTGTCAATC AGAGGAGACT TCAGGACAAC AGTGGAGTAC 
CTTATAAAAC TGCTGGAAAC AGAAAGTTTC AGAAACAATG ACATTGATAC TGGTTGGCTG 
GATCACCTTA TTGCTGATAA AGTGCAGGCA GAGAGGCCAG ATACTATGTT AGGTGTGGTA 
TGCGGAGCTC TACAAGTGGC TGATGCAAGT TTTAGAGAAA GCATGTCTGA CTTCCTGCAT 
TCACTGGAAA GGGGTCAGGT GTTGCCGGCT GCTAGTTTGG TCAACACAGT TAATGTTGAT 
CTGATCTATG ATGGCGTCAA ATACTGCCTG AAGGTAGCCC GTCAGTCACC TACGACATAT 
GTTATCATAA TGAATGACTC CGATATAGAA GTTGATGTCC ATAGGCTCAG TGATGGTGGT 
CTACTGCTTT CCTATGGCGG CAGCAGCTAC ACAACCTACA TGAAGGAGGA GATTGATAGG 
TACCGTGTCA CAGTGGGCAA TAAAACGTGC GTGTTTGAGA AGGAGCGAGA CCCTACGGTG 
CTCCGTTCGC CCTCTGCTGG CAAGCTTTTG CAGTATGTAG TCACTGATGG TTCACATGTT 
TCGGCTTCCC AGCCTTATGC TGAAATTGAG GTGATGAAGA TGGTGATGAC CCTGCATGTC 
CAGCATTCTG GTTGTATTCG CTTTTTAAAA AGACCAGGAA CAGTATTGGA GCCTGGCTGT 
ATAGTGGCCC TAATGGACCT GGATGACCCC AGCTGTATTC ATCAGGTGAA ACCAAACACA 
GAGCCATTAC CAGCTCAGGA GCCATTACCC ATGGTAGGAG AGAGGCTTCA CCAGGTGTTC 
CACAATGTTC TGGAGAACCT GGTGAAAATT ATGGATGGAT ATTGTCTGCC AGAGCCATAT 
TTTAGTCAGA AATTGAAGAA CTGGGTCGAC ACGCTGATGA AGACGCTTCG GGATCCGTCT 
CTGCCTCTAC TTGAACTGCA GGAGATCATG ACCAGCGTGG CAGGGCGAAT TCCAGTTACT 
GTGGAGAAGG CAATTCGAAA GGTCATGGCA CAGTACGCCA GTAACATTAC CTCTGTGCTC 
TGTCAGTTCC CCAGCCAAAG GATAGCAAAT ATACTTGATA GCCATGCTGC CACACTTCAA 
AGGAAAGCAG ACCGGGAAAT TTTCTTCATG AACACCCAGA GCATTGTGCA ATTGGTGCAA 
AGATACCGTA GCGGCATTAG GGGCTACATG AAATCTGTTG TTCTGGATCT TTTGAAACAC 
TACCTTCAGG TGGAGATGCA ATTTCAGCAA GCTCACTATG ACAAGTGTGT GATCAATCTC 
AGAGAGCAGT ATAAGCCGGA CATGACCCCA GTGCTGGAGT GCATCTTTTC TCATGCTCAG 
GTGTCCAAGA AGAACATCCT CGTCACCATG CTCATAGACC AGCTGTGTGG CCGGGACCCA 
ATGCTTGCTG ATGAGCTCAT GGTCATTCTG GATGAGCTTA CACAACTCAG TAAAATGGAA 
AACTCTAAAG TGGCACTTAG GGCCAGACAG GTGTTGATCG CCTCTCATTT GCCCTCATAT 
GAACTCAGGC ACAACCAGGT GGAATCCATC TTTTTATCAG CTATTGATAT GTATGGGCAT 
CAGTTTTGTC CTGAAAACCT CAAGAAACTA ATCCTATCTG AGACCTCCAT CTTTGACGTC 
TTGCCCAGTT TTTTCTACCA CAACAACCGT GTGGTTTGCA TGGCTGCATT AGAGGTTTAT 
GTACGGAGGG CTTATATAGC TTACGAGCTA AACAGTCTTC AGCACCATCA GCTGCAGGAT 
GGGACATGTG CGGTGGATTT CCAGTTCATG TTACCATCAT CCCACCCAAA CAGAGAGCCT 
AACGCCACAG TGAGCCAGCA CATGCCCAAA TCCACAGGGA GCAGTCCTAC TTTGAACAGA 
CTGACCCTTC CTGTCAACGA TTCGGGAGAG TTTCCGGCAA TGCGCCGTCA AGGCAGTGAG 
CTCTTTCTAG AGGGGGCGCT TTCACCCCCC TGCCAGAGGA TGGGAGCCAT GGTTGCCTTC 
CATAGCTTTG ACCACTTCAA AAGGTGCTTT GATGAAGTGA TCTGTCGCTT TGTGGATCCG 
CTGTGTGAAA GTTCTCTGTT TTTTGATGGC TGTTCCACCT TCTGTGATGG GGAGAGCTGT 
AAAAACATGA AGGAAAACCC CATTCACATC ATAAATGTGT CAATTAAACA AGCAGACACA 
GAAGATGATG ATGCTTTGGT CACAGCTTTC ACTGCCTTTG CTCACTCCAA AAAATCGCTA 
CTCTATGATT ACGGAATCAG AAGGGTGACA TTTTTAGTTG CACAAAAGCG GGAGTTCCCA 
AAATACTTCA CGTTTAGGGC CAGAGATGAA TTCCATGAAG ACAGAATCTA CCGTAACCTG 
GAGCCTGCTC TGGCCTTCCA GCTGGAGCTG AACCGCATGC GTAACTTTGA CCTTAAGGCT 
GTTCCCTGTG CTCACCACCG CATGCAGCTG TACTTGGGTG CTGCTCGTGT GGAAGAGGGC 
GCAGAGGTCA CTGATTATCG CTTCTTTATC AGAGCCATCA TTCGCCACTC TGACCTCATC 
ACCAAGGAGG CCTCCTTTGA GTATTTGCAG AATGAGGGTG AGAGGCTGTT GCTGGAGGCG 
ATGGATGAGC TGGAGGTGGC CTTTAGTAAC ACACCCACTC GGACTGACTG CAATCACATC 
TTCCTCAATT TTGTACCCAC TGTTATTATG GACCCATACA AGATAGAGGA GTCTGTCCGC 
TCCATGGTGA TGCGATACGG TAGTCGACTG TGGAAGCTGC GAGTTCTCCA GGCTGAGCTG 
AAAATCAACA TCCGCCTCAC CACCACTGGA GATGTTATTC CCATACGCCT CTTTCTCACC 
AATGAGTCTG GATACTATCT GGACATCAGC TTGTATAAAG AGGTCAATGA CCCATCCAGT 
GGACAGATAA TGTTCCAGTC GTATGGAGAC AAACAAGGCC CGCTGAACGG CATGCTCATA 
AACACACCAT ACGTCACAAA GGATTTACTC CAGGCCAAGC GCTTCCAGGC TCAGAGCCTC 
GGCACCACAT ATGTCTATGA CTTCCCTGAG ATGTTTAGAC AGGCTTTGTT TAAGCTTTGG 
GGACCAGGAG ACAGTTATCC TAAAGATGTG TTGATGTGTA ATGAGCTGGT CCTGGATTCT 
CAGGGGAACC TTGTGCAGAT GAACAGACTA CCAGGAGACA ATGAGGTTGG AATGGTAGCT 
TTCCGGATGA GGATGAAGAC TCCAGAGTAC CCAGAGGGCC GAGATATCAT TGTGATCTGT 
AATGATATTA CCCATATGAT TGGCTCTTTT GGGCCTCAGG AGGATCAGCT TTTTATGCGA 
GCATCTGAGC TGGCCAGAGC GGAGGGCATC CCGCGCATCT ACATCTCAGC CAACAGCGGA 
GCTCGAATCG GCCTGGCAGA GGAGATCCGC CACATGTTTC AGGTGGCTTG GATCGACCCT 
GAAGACCCAT ATAAGGGTTT TAAGTACCTG TATCTGACTC CTCAAGACTA CACCCGCATC 
AGTTCCTCCA ATTCTGTCCA TTGTCACCAT GTAGAGGAGG GAGGAGAGTC CAGATACATC 
CTCACAGACA TCATAGGAAA GGAAGAGGGT ATTGGAGTGG AGAATCTGAG GGGCTCGGGC 
ACAATAGCTG GAGAAACTTC TCAGGCTTAT AAAGAAATAA TCACTATCAG CATGGTCACG 
TGTCGGGCTA TAGGAATCGG TGCCTATCTG GTACGTCTAG GACAAAGAGT CATTCAAGTG 
GAAAACTCCC ATATCATCCT GACTGGAGCC GGGGCATTAA ACAAGGTGCT GGGTCGTGAA 
GTCTACACCT CCAATAACCA GCTTGGTGGG GTTCAGATCA TGCACAACAA TGGAGTGACA 
CACTCCACCG TGCCAGATGA CTTTGAAGGA GTGCTCACTA TTCTACAGTG GTTATCCTAC 
ATGCCAAAGA GCAATCAAAG TCCAGTCCCA ATAAAGCCTG CCACTGATCC AGTCGACAGA 
GAGATCGATT TTGTTCCCAC AAAAGCCCCT TATGACCCTC GCTGGCTGCT CTGTGGACGA 
CCCCATCCCA CTGTGAAGGG AGCATGGCAG AGTGGATTCT TTGATCATGG TTCATTTTTG 
GAGATAATGG CTACATGGGC ACAGACAGTG GTGGTGGGCC GAGCCAGGCT TGGTGGGATT 
CCTCTTGGGG TCATTGCTGT GGAGACCCGC ACAGTCGAGC TTGCCATTCC AGCAGATCCT 
GCCAACTTAG ACTCAGAGGC CAAGCTCCTC CAGCAAGCTG GTCAGGTGTG GTTTCCAGAT 
TCTGCATATA AAACGGCTCA GGCTATTGAG GATTTCAATA GAGAGAAACT TCCCCTTATG 
GTGTTTGCCA ACTGGCGAGG ATTTTCTGGA GGCATGAAAG ATATGTACGA CCAGGTGCTC 
AAGTTTGGCT CTTATATCGT GGATGCCCTG CGAGAATTCA GCCAGCCTGT GCTGGTTTAC 
ATTCCTCCTA ATGCTGAGCT GAGAGGAGGA TCATGGGTCG TGATCGACCC TACTATAAAC 
CTTCAGCACA TGGAGCTCTA TGCAGATCGA GAAAGCAGAG GGGGCGTTTT GGAGGCTGAG 
GGCACAGTGG AGATCAAATT CAGAAAAAAA GACCTGCTAA AGACCATGCG AAGGATTGAT 
CCAGTGTATT CTCGTCTGGC TGAGCAGCTA GGCAAACCAG ACTTACCGAG CCAGGAGCGT 
AAAGACCTGG AGGCAAAACT AAAGTCTAGA GAGGAGTTCC TTCTTCCCAT CTACCACCAG 
GTGGCAGTGC AGTTTGTTGA CCTTCATGAC ACTCCAGGAC GGATGCAGGA AAAGGGAGTC 
ATCACGGACA TCCTGGACTG GAAGACTGCT AGGTCATTCT TTTACTGGCG TCTGAGGCGA 
CTGCTGTTGG AGGAAGTGGT AAAGAAGGAG ATCATGCAGG CCAACCAAGA CCTGAGCAAC 
GGACACATAC AGTCCATGCT GCGCCGCTGG TTTGTGGAGA CAGAAGGAGC TGTAAAAGCA 
TATCTGTGGG ACAATAACAA AGTGGTGGTG GAGTGGCTGG AAAACAACCT GTCCCAGCAG 
GACGGAACAC GTTCGGTCAT CAGAGAAAAC ATCAAATGCA TAAAGAGGGA CTACGCTCTC 
AAACACATCC GCAGCTTAGT CCAAGCCAAT CCAGAAGTGA CTATGGACTG CATCATTCAC 
ATGGCACAGA ACATTACGCC TTCACAGAGA GCCAAAGTCT GTCACCTGCT GGCCACCATG 
GACAACTCCA CCACCAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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acacb cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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