gigfy2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gigfy2
Entrez Gene ID	561830
Full Name	GRB10 interacting GYF protein 2
Synonyms	fb57e02,gigfy2,im:6911549,wu:fb57e02,zgc:111944
General protein information	Preferred Names GRB10 interacting GYF protein 2 Names GRB10-interacting GYF protein 2 PERQ amino acid-rich with GYF domain-containing protein 2 trinucleotide repeat-containing gene 15 protein
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017358653.1	XP_017214142.1	PERQ amino acid-rich with GYF domain-containing protein 2 isoform X1
NM_001030161.2	NP_001025332.2	GRB10-interacting GYF protein 2
XM_017358654.1	XP_017214143.1	PERQ amino acid-rich with GYF domain-containing protein 2 isoform X2
XM_017358657.1	XP_017214146.1	PERQ amino acid-rich with GYF domain-containing protein 2 isoform X3
XM_017358654.2	XP_017214143.1	GRB10-interacting GYF protein 2 isoform X1

Pathways from BioSystems

Homologies

	GIGYF2	XP_001114252.2
Mus musculus (house mouse)	Gigyf2	NP_666224.3
Gallus gallus (chicken)	GIGYF2	NP_001239057.1
Danio rerio (zebrafish)	gigfy2	NP_001025332.1
Xenopus tropicalis (tropical clawed frog)	gigyf2	XP_002933400.2
Rattus norvegicus (Norway rat)	Gigyf2	XP_006245571.1
Homo sapiens (human)	GIGYF2	NP_001096616.1
Canis lupus familiaris (dog)	GIGYF2	XP_861928.1
Pan troglodytes (chimpanzee)	GIGYF2	XP_001148316.1
Bos taurus (cattle)	GIGYF2	NP_001095347.1

Gene Ontology
Bibliography

Related articles in PubMed

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine induced Parkinson's disease in zebrafish.
Sarath Babu N, Murthy ChL, Kakara S, Sharma R, Brahmendra Swamy CV, Idris MM
Proteomics16(9)1407-20(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Mutational screening and zebrafish functional analysis of GIGYF2 as a Parkinson-disease gene.
Guella I, Pistocchi A, Asselta R, Rimoldi V, Ghilardi A, Sironi F, Trotta L, Primignani P, Zini M, Zecchinelli A, Coviello D, Pezzoli G, Del Giacco L, Duga S, Goldwurm S
Neurobiology of aging32(11)1994-2005(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following gigfy2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gigfy2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa43602	NM_001030161.2 Latest version!	Danio rerio GRB10 interacting GYF protein 2 (gigyf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa56295	XM_017358653.1 Latest version!	Danio rerio GRB10 interacting GYF protein 2 (gigfy2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa56296	XM_017358654.2 Latest version!	Danio rerio GRB10 interacting GYF protein 2 (gigfy2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa56297	XM_017358657.1 Latest version!	Danio rerio GRB10 interacting GYF protein 2 (gigfy2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
View Old Accession Versions >>
ODa56296	XM_017358654.1	Danio rerio GRB10 interacting GYF protein 2 (gigfy2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gigfy2 ( NM_001030161.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001025332.2 Danio rerio GRB10 interacting GYF protein 2 (gigyf2), mRNA.

Price & Availability↑

CloneID	ODa43602
Clone ID Related Accession (Same CDS sequence)	NM_001030161.2
Accession Version	NM_001030161.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	GRB10-interacting GYF protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX890608.15, BX890560.9 and BX677676.6. On Aug 25, 2015 this sequence version replaced NM_001030161.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC098603.1, GDQH01010399.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGAAA CCCAGACACT CAACTTTGGG CCAGAATGGC TCCGTGCTCT GGCTGGTGGG 
GTGGGCAGCA GCATTGTGGC CTCCCCTCCG CTCTCGCCTG CATTGCCAAA GTATAAACTT 
GCAGACTATC GCTACGGGAG AGAAGAGATG CTAGCACTTT ATGTAAAGGA TAACATGATT 
CCGGTAGATC TTCATGATAA GGAATTCCTA CCTATTTTAC AAGAGGAGCC CCTGCCACCT 
TTGGCATTAG TCCCTTTTAC AGAGGAGGAA CAGAGAAATT TCTCCATGTC TGTAAACAGT 
GCAGCAGTTC TCCGTCTGAC AGGAAGGGGG GGAGGGACGG TGGCAGGGGC ACCTAGAGGC 
CGAAGTTCTT CACGAGGGCG AGGGAGAGGC AGAGGAGATG GTTTTTACCA AAGAAGTTTT 
GATGATGTGG AAGGAGGTTT CGGTCGTGGA GGGAGAGAGA TGCATCGCTC CCAGAGTTGG 
GAGGAGAGGG GAGACAGAAG GTTTGAAAAG CCAGGTCGAA AAGATCCAGA TGGAGCACCA 
GCCCATTTTC CACTCAATCA CATAAGGGCA AACTATGAGG ACCCTGCTGC AGTGAATGCC 
CGAAAGTTCA TTCATGCAGA GTGTGACAAC TGGCGCACCA CTCGAGATGA GCTGAATGGA 
GAGGAGGATG ATAACGGATG GAGGCTTGCA GGCGCTCGTC GGGAAAATGA GCGATGGTGT 
CCTCCAAGTC CAGATGGTCC ACGATCAGCA GGGTGGCGGG AACACCCAGA TCAACGTCGA 
CGCTTTGGTT TTGGGGATGA GGAACGCAGT GGTTACCGTA GGCCTCGGTC GGGCAGCGGG 
AGCGCAGAGG AGGAGAGGGA CAGTTTACCA GAATGGTGTC TGGAAGATGC AGAGGAGGAG 
ACAGGGACGT TTGACTCCTC AGGGGCCTTC CTCTCACTTA AGGTGAGGGC GCTGGAAAAA 
GCTCCTAAGG AGCCAATCCT GGAAGAGGCG GAGCTGGACT TCCGGCCATT GGAGGAGAAT 
GACGAATATG CAGAAAAGGA TGACAGTGAA ACTGAACAAA CCAAAGATAC TGACACAAAT 
ACTAGGCACG AAAGTGATCG AAATGAAGAA AACTGTAAAT CAGAAGAGCC GTCGCCAGTT 
GCGGTACCAT TTTCAGCAGT AGTTACGCCT CCCAAAGCCA CCACCCCTGC ACCCATTCAG 
CCTGTACACC TAGAAAAAGC TGAAGATAAG GAGAGACCGT CAGAGAGAAC AACATTACCA 
GAAATCAGAC ATGAACTGAG TAAAGCACCA CTACACACAG CCCTATCTAA CAGCATTGTG 
GAAGCCATTT CCATACCGCA TGTCGCAAAC AAACTCCCAG ATCTTCCGGT CCCAGCGCCT 
TCAGTTCTGC CTGTCAAGTC AGTGCCCCCT CAATCCCAAC AGGTCAAGCC TATAGAGATG 
CCAGTGTCAG TTCCTCCTGC TCTGCTACGC TCCACAGGCA GTGTGGGTCC TATCAGTCGA 
CCCTCTGCCC TGCCATCGGA CTTGGATGAG GATGAGGGAC TCAAGCACTT TGAGCAGGAA 
GCTGAGAAAA TGGTGGCGTA CCTCCAGGAT GGTGGCGTAG ATGATGAACG TTTGGCCTCT 
AAAATTGGAC CCAAACCATC AGCTCTTCCA ATCACAAATG AAGCTGCATT CAAATGGTTT 
TACAAAGATC CGCAGGGGGA AATCCAAGGA CCATTTAATA ATCAGGAGAT GTCTGAGTGG 
TTTCAGGCGG GCTATTTCAC CATGACCTTG CAGGTAAAGC GAGGATGCGA CGAGATGTTC 
CAGCCTCTGG GCGAGATGAT TAAGTTGTGG GGACGAGTGC CCTTTACACC GGGCCCGACA 
CTACCTCCCA TACTGGGAGA TGCAGATCAA GAAAGGATGA AGAGACAACA GGAGATTAAT 
GCTCTAAATA TGTATCAGCT ACAGCAGATG CAATATCAGT ATTTACTCAG GCAACAATAT 
GCTCTGCAGC AGAAAGTGTT AAACTCTGCT CCTCCTCCTC CTCCACCACC ACAGCAGCAG 
CTCAATCTTC TTCTCCATCA GGCGCTGAAG ATCAGAACTC CTGAGCCGCA GCAGAGCCTC 
TTACCTCCCG TCACACGCTC CATGTCGGTT CCAGACTCGG GTTCTGTTTG GGAAATGCAG 
AACCCGTCCA CTCAGGCATC CTGTTCTCCA AACATGCAGC CCGCCGCTCC CAGCACTTGG 
GAAGGGAGTA GCGTATGGGA TCTGCCACTA GACAATATGC CTCAAGCGTC TTCCATTGAA 
CAGCTGCAGC TGGAGAAAGC CAAAGCTTTG AAGCTGGAGA TGGAGAGACG GGAGGCAGAG 
CTTCGGGCCA AAAGGGAAGA AGAGGAGAGG AAGCGTTTGG AAGAGGCTCT GCGTGCGAGA 
CAAGAGGAAG AGAGGAAGCG TTTAGAGGAA GAAGAGCTGG CACGACGCAA GCAGGAGGAG 
GCTCTGAAGA GACAGCGGGA GCAGGAGGAG GCGCAGCGGA GGAAAAAGGA AGAGGAGGAA 
CGGTTAGCTC AGGAGGAGGC TCTCCGGCGA CTGGAGGAGC GGAGGAGAGA AGAAGAGGAG 
AGGAGACAAA GGGAAGAATT CCTCCGCAAA CAGCAGGAAG AGGAGCGGAG GAAGCAGGAA 
GAACTGGAAG CTCAGAGGCG GCGTGAGGAA GAGAAGAGGC TGGAGGAGGA GGCTGCGGCC 
GCTGCAGCAG CACTGCTCAG ACAACAGCAG GAGGAGCAGA AGAAGAGAGA GCAGGAGGCG 
CAGAGACAGC AGGAGCTGCA GAGACAGAGA CAGCAGCAAC AGGAGGCACT CCGACGACTC 
CAGCAGCAGC AGCAACAACA GCAGCTCGCA CAAATGAAGC TACCTTCATC CTCAAAGTGG 
GGTCAGCAGT CGACCACAGC AAACAGTCTC TCACAGTCTC AAAATGCTCT TTCGCTCGCT 
GAGATCCAGA AACTGGAGGA GGAGAGAGAA CGACAGACTC GAGAAGAGCA GAGACGTCAG 
CAGCAGGAGC TCCAGCGGGT CCAGCAGCAG CAGCCTCAGA CCAAGCTCCC TGGCTGGGGC 
AGTGTGGCTA AGCAGCCCAT GGCCACTAAA TCCCTGTTGG AGATCCAGAG AGAGGAAGCA 
CAGCAGATGA AGCAGCGGAA AGATCAGCAG CAGCAGCAAC AACAACAACA ACAACCACCT 
CCACAGCCTC AGCCTCAGCA ACATTCAACC AACACCCAGC AAAATCGCAC ACAAAACCGG 
GCGGCCATCA ATACGTCAGT GTGGGGTTCT GTCAATAATG TGAGCTCAAA CTGGATGATG 
GACAGCAGTG TATGGGGCGA CACGCAGAAC TCTAATATTG GCTTCTGGGA TGAAGCAGTA 
AAGGAGGCCG CTCCACCTCA AACCACTCGC AAGAGCCACA CTCCGAAAAA CAAGGGCAAC 
GCTAACCTCA GTAATAGCAG CAGCGGTAAA GCCAGTAAAA AGGTGGAAGA GGAGGAGAAG 
CTACTGAAAC TGTTCCAGGG AGCCAATAAG AACCAGGACG GCTTTATGCA GTGGTGTGAA 
CAGACCCTGC ACACTCTCAA CACAGCCAAT AACCTCGATG TTCCCACTTT TGCATCGTTC 
CTGAAGGAAG TGGACTCTCC GTATGAGGTG CACGATTATG TACGAGCTTA TCTGGGTGAC 
ACTCCACAGG CCAAGGACTT CGCCAAGCAG TTCCTGGAGC GCCGTGCCAA ACAGAACGAC 
AACCAGCAGA AACCTCAGCA GGGCCAGCAA CAAAAACAGC AGGAGTCGGT GTGGGGAATA 
AGAGAAGTGC CACAGTCAGT CCTGCTTCAA CAACAACAAC AACAACAATT ACAGCAGCAG 
CAACAGCAGC GGTTTGAAAC GGTCACTTCC GGCAAGAAGA AGAAGAAACA GAAGATGGTA 
CGAGCCGACC CAAGCCTTCT AGGTTTTTCT GTCAATGCCT CCTCTGAGAG ACTCAATATG 
GGCGAGATCG AGACGGTTGA AGACTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025332.2
CDS	37..4026
Translation	MAETQTLNFGPEWLRALAGGVGSSIVASPPLSPALPKYKLADYRYGREEMLALYVKDNMIPVDLHDKEFLPILQEEPLPPLALVPFTEEEQRNFSMSVNSAAVLRLTGRGGGTVAGAPRGRSSSRGRGRGRGDGFYQRSFDDVEGGFGRGGREMHRSQSWEERGDRRFEKPGRKDPDGAPAHFPLNHIRANYEDPAAVNARKFIHAECDNWRTTRDELNGEEDDNGWRLAGARRENERWCPPSPDGPRSAGWREHPDQRRRFGFGDEERSGYRRPRSGSGSAEEERDSLPEWCLEDAEEETGTFDSSGAFLSLKVRALEKAPKEPILEEAELDFRPLEENDEYAEKDDSETEQTKDTDTNTRHESDRNEENCKSEEPSPVAVPFSAVVTPPKATTPAPIQPVHLEKAEDKERPSERTTLPEIRHELSKAPLHTALSNSIVEAISIPHVANKLPDLPVPAPSVLPVKSVPPQSQQVKPIEMPVSVPPALLRSTGSVGPISRPSALPSDLDEDEGLKHFEQEAEKMVAYLQDGGVDDERLASKIGPKPSALPITNEAAFKWFYKDPQGEIQGPFNNQEMSEWFQAGYFTMTLQVKRGCDEMFQPLGEMIKLWGRVPFTPGPTLPPILGDADQERMKRQQEINALNMYQLQQMQYQYLLRQQYALQQKVLNSAPPPPPPPQQQLNLLLHQALKIRTPEPQQSLLPPVTRSMSVPDSGSVWEMQNPSTQASCSPNMQPAAPSTWEGSSVWDLPLDNMPQASSIEQLQLEKAKALKLEMERREAELRAKREEEERKRLEEALRARQEEERKRLEEEELARRKQEEALKRQREQEEAQRRKKEEEERLAQEEALRRLEERRREEEERRQREEFLRKQQEEERRKQEELEAQRRREEEKRLEEEAAAAAAALLRQQQEEQKKREQEAQRQQELQRQRQQQQEALRRLQQQQQQQQLAQMKLPSSSKWGQQSTTANSLSQSQNALSLAEIQKLEEERERQTREEQRRQQQELQRVQQQQPQTKLPGWGSVAKQPMATKSLLEIQREEAQQMKQRKDQQQQQQQQQQPPPQPQPQQHSTNTQQNRTQNRAAINTSVWGSVNNVSSNWMMDSSVWGDTQNSNIGFWDEAVKEAAPPQTTRKSHTPKNKGNANLSNSSSGKASKKVEEEEKLLKLFQGANKNQDGFMQWCEQTLHTLNTANNLDVPTFASFLKEVDSPYEVHDYVRAYLGDTPQAKDFAKQFLERRAKQNDNQQKPQQGQQQKQQESVWGIREVPQSVLLQQQQQQQLQQQQQQRFETVTSGKKKKKQKMVRADPSLLGFSVNASSERLNMGEIETVEDF

Target ORF information:

RefSeq Version	NM_001030161.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio GRB10 interacting GYF protein 2 (gigyf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001030161.2

ORF Insert Sequence:

ATGGCGGAAA CCCAGACACT CAACTTTGGG CCAGAATGGC TCCGTGCTCT GGCTGGTGGG 
GTGGGCAGCA GCATTGTGGC CTCCCCTCCG CTCTCGCCTG CATTGCCAAA GTATAAACTT 
GCAGACTATC GCTACGGGAG AGAAGAGATG CTAGCACTTT ATGTAAAGGA TAACATGATT 
CCGGTAGATC TTCATGATAA GGAATTCCTA CCTATTTTAC AAGAGGAGCC CCTGCCACCT 
TTGGCATTAG TCCCTTTTAC AGAGGAGGAA CAGAGAAATT TCTCCATGTC TGTAAACAGT 
GCAGCAGTTC TCCGTCTGAC AGGAAGGGGG GGAGGGACGG TGGCAGGGGC ACCTAGAGGC 
CGAAGTTCTT CACGAGGGCG AGGGAGAGGC AGAGGAGATG GTTTTTACCA AAGAAGTTTT 
GATGATGTGG AAGGAGGTTT CGGTCGTGGA GGGAGAGAGA TGCATCGCTC CCAGAGTTGG 
GAGGAGAGGG GAGACAGAAG GTTTGAAAAG CCAGGTCGAA AAGATCCAGA TGGAGCACCA 
GCCCATTTTC CACTCAATCA CATAAGGGCA AACTATGAGG ACCCTGCTGC AGTGAATGCC 
CGAAAGTTCA TTCATGCAGA GTGTGACAAC TGGCGCACCA CTCGAGATGA GCTGAATGGA 
GAGGAGGATG ATAACGGATG GAGGCTTGCA GGCGCTCGTC GGGAAAATGA GCGATGGTGT 
CCTCCAAGTC CAGATGGTCC ACGATCAGCA GGGTGGCGGG AACACCCAGA TCAACGTCGA 
CGCTTTGGTT TTGGGGATGA GGAACGCAGT GGTTACCGTA GGCCTCGGTC GGGCAGCGGG 
AGCGCAGAGG AGGAGAGGGA CAGTTTACCA GAATGGTGTC TGGAAGATGC AGAGGAGGAG 
ACAGGGACGT TTGACTCCTC AGGGGCCTTC CTCTCACTTA AGGTGAGGGC GCTGGAAAAA 
GCTCCTAAGG AGCCAATCCT GGAAGAGGCG GAGCTGGACT TCCGGCCATT GGAGGAGAAT 
GACGAATATG CAGAAAAGGA TGACAGTGAA ACTGAACAAA CCAAAGATAC TGACACAAAT 
ACTAGGCACG AAAGTGATCG AAATGAAGAA AACTGTAAAT CAGAAGAGCC GTCGCCAGTT 
GCGGTACCAT TTTCAGCAGT AGTTACGCCT CCCAAAGCCA CCACCCCTGC ACCCATTCAG 
CCTGTACACC TAGAAAAAGC TGAAGATAAG GAGAGACCGT CAGAGAGAAC AACATTACCA 
GAAATCAGAC ATGAACTGAG TAAAGCACCA CTACACACAG CCCTATCTAA CAGCATTGTG 
GAAGCCATTT CCATACCGCA TGTCGCAAAC AAACTCCCAG ATCTTCCGGT CCCAGCGCCT 
TCAGTTCTGC CTGTCAAGTC AGTGCCCCCT CAATCCCAAC AGGTCAAGCC TATAGAGATG 
CCAGTGTCAG TTCCTCCTGC TCTGCTACGC TCCACAGGCA GTGTGGGTCC TATCAGTCGA 
CCCTCTGCCC TGCCATCGGA CTTGGATGAG GATGAGGGAC TCAAGCACTT TGAGCAGGAA 
GCTGAGAAAA TGGTGGCGTA CCTCCAGGAT GGTGGCGTAG ATGATGAACG TTTGGCCTCT 
AAAATTGGAC CCAAACCATC AGCTCTTCCA ATCACAAATG AAGCTGCATT CAAATGGTTT 
TACAAAGATC CGCAGGGGGA AATCCAAGGA CCATTTAATA ATCAGGAGAT GTCTGAGTGG 
TTTCAGGCGG GCTATTTCAC CATGACCTTG CAGGTAAAGC GAGGATGCGA CGAGATGTTC 
CAGCCTCTGG GCGAGATGAT TAAGTTGTGG GGACGAGTGC CCTTTACACC GGGCCCGACA 
CTACCTCCCA TACTGGGAGA TGCAGATCAA GAAAGGATGA AGAGACAACA GGAGATTAAT 
GCTCTAAATA TGTATCAGCT ACAGCAGATG CAATATCAGT ATTTACTCAG GCAACAATAT 
GCTCTGCAGC AGAAAGTGTT AAACTCTGCT CCTCCTCCTC CTCCACCACC ACAGCAGCAG 
CTCAATCTTC TTCTCCATCA GGCGCTGAAG ATCAGAACTC CTGAGCCGCA GCAGAGCCTC 
TTACCTCCCG TCACACGCTC CATGTCGGTT CCAGACTCGG GTTCTGTTTG GGAAATGCAG 
AACCCGTCCA CTCAGGCATC CTGTTCTCCA AACATGCAGC CCGCCGCTCC CAGCACTTGG 
GAAGGGAGTA GCGTATGGGA TCTGCCACTA GACAATATGC CTCAAGCGTC TTCCATTGAA 
CAGCTGCAGC TGGAGAAAGC CAAAGCTTTG AAGCTGGAGA TGGAGAGACG GGAGGCAGAG 
CTTCGGGCCA AAAGGGAAGA AGAGGAGAGG AAGCGTTTGG AAGAGGCTCT GCGTGCGAGA 
CAAGAGGAAG AGAGGAAGCG TTTAGAGGAA GAAGAGCTGG CACGACGCAA GCAGGAGGAG 
GCTCTGAAGA GACAGCGGGA GCAGGAGGAG GCGCAGCGGA GGAAAAAGGA AGAGGAGGAA 
CGGTTAGCTC AGGAGGAGGC TCTCCGGCGA CTGGAGGAGC GGAGGAGAGA AGAAGAGGAG 
AGGAGACAAA GGGAAGAATT CCTCCGCAAA CAGCAGGAAG AGGAGCGGAG GAAGCAGGAA 
GAACTGGAAG CTCAGAGGCG GCGTGAGGAA GAGAAGAGGC TGGAGGAGGA GGCTGCGGCC 
GCTGCAGCAG CACTGCTCAG ACAACAGCAG GAGGAGCAGA AGAAGAGAGA GCAGGAGGCG 
CAGAGACAGC AGGAGCTGCA GAGACAGAGA CAGCAGCAAC AGGAGGCACT CCGACGACTC 
CAGCAGCAGC AGCAACAACA GCAGCTCGCA CAAATGAAGC TACCTTCATC CTCAAAGTGG 
GGTCAGCAGT CGACCACAGC AAACAGTCTC TCACAGTCTC AAAATGCTCT TTCGCTCGCT 
GAGATCCAGA AACTGGAGGA GGAGAGAGAA CGACAGACTC GAGAAGAGCA GAGACGTCAG 
CAGCAGGAGC TCCAGCGGGT CCAGCAGCAG CAGCCTCAGA CCAAGCTCCC TGGCTGGGGC 
AGTGTGGCTA AGCAGCCCAT GGCCACTAAA TCCCTGTTGG AGATCCAGAG AGAGGAAGCA 
CAGCAGATGA AGCAGCGGAA AGATCAGCAG CAGCAGCAAC AACAACAACA ACAACCACCT 
CCACAGCCTC AGCCTCAGCA ACATTCAACC AACACCCAGC AAAATCGCAC ACAAAACCGG 
GCGGCCATCA ATACGTCAGT GTGGGGTTCT GTCAATAATG TGAGCTCAAA CTGGATGATG 
GACAGCAGTG TATGGGGCGA CACGCAGAAC TCTAATATTG GCTTCTGGGA TGAAGCAGTA 
AAGGAGGCCG CTCCACCTCA AACCACTCGC AAGAGCCACA CTCCGAAAAA CAAGGGCAAC 
GCTAACCTCA GTAATAGCAG CAGCGGTAAA GCCAGTAAAA AGGTGGAAGA GGAGGAGAAG 
CTACTGAAAC TGTTCCAGGG AGCCAATAAG AACCAGGACG GCTTTATGCA GTGGTGTGAA 
CAGACCCTGC ACACTCTCAA CACAGCCAAT AACCTCGATG TTCCCACTTT TGCATCGTTC 
CTGAAGGAAG TGGACTCTCC GTATGAGGTG CACGATTATG TACGAGCTTA TCTGGGTGAC 
ACTCCACAGG CCAAGGACTT CGCCAAGCAG TTCCTGGAGC GCCGTGCCAA ACAGAACGAC 
AACCAGCAGA AACCTCAGCA GGGCCAGCAA CAAAAACAGC AGGAGTCGGT GTGGGGAATA 
AGAGAAGTGC CACAGTCAGT CCTGCTTCAA CAACAACAAC AACAACAATT ACAGCAGCAG 
CAACAGCAGC GGTTTGAAAC GGTCACTTCC GGCAAGAAGA AGAAGAAACA GAAGATGGTA 
CGAGCCGACC CAAGCCTTCT AGGTTTTTCT GTCAATGCCT CCTCTGAGAG ACTCAATATG 
GGCGAGATCG AGACGGTTGA AGACTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gigfy2 ( XM_017358653.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214142.1 Danio rerio GRB10 interacting GYF protein 2 (gigfy2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa56295
Clone ID Related Accession (Same CDS sequence)	XM_017358653.1
Accession Version	XM_017358653.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	PERQ amino acid-rich with GYF domain-containing protein 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAAA CCCAGACACT CAACTTTGGG CCAGAATGGC TCCGTGCTCT GGCTGGTGGG 
GTGGGCAGCA GCATTGTGGC CTCCCCTCCG CTCTCGCCTG CATTGCCAAA GTATAAACTT 
GCAGACTATC GCTACGGGAG AGAAGAGATG CTAGCACTTT ATGTAAAGGA TAACATGATT 
CCGGTAGATC TTCATGATAA GGAATTCCTA CCTATTTTAC AAGAGGAGCC CCTGCCACCT 
TTGGCATTAG TCCCTTTTAC AGAGGAGGAA CAGAGAAATT TCTCCATGTC TGTAAACAGT 
GCAGCAGTTC TCCGTCTGAC AGGAAGGGGG GGAGGGACGG TGGCAGGGGC ACCTAGAGGC 
CGAAGTTCTT CACGAGGGCG AGGGAGAGGC AGAGGAGATG GTTTTTACCA AAGAAGTTTT 
GATGATGTGG AAGGAGGTTT CGGTCGTGGA GGGAGAGAGA TGCATCGCTC CCAGAGTTGG 
GAGGAGAGGG GAGACAGAAG GTTTGAAAAG CCAGGTCGAA AAGATCCAGA TGGAGCACCA 
GCCCATTTTC CACTCAATCA CATAAGGGCA AACTATGAGG ACCCTGCTGC AGTGAATGCC 
CGAAAGTTCA TTCATGCAGA GTGTGACAAC TGGCGCACCA CTCGAGATGA GCTGAATGGA 
GAGGAGGATG ATAACGGATG GAGGCTTGCA GGCGCTCGTC GGGAAAATGA GCGATGGTGT 
CCTCCAAGTC CAGATGGTCC ACGATCAGCA GGGTGGCGGG AACACCCAGA TCAACGTCGA 
CGCTTTGGTT TTGGGGATGA GGAACGCAGT GGTTACCGTA GGCCTCGGTC GGGCAGCGGG 
AGCGCAGAGG AGGAGAGGGA CAGTTTACCA GAATGGTGTC TGGAAGATGC AGAGGAGGAG 
ACAGGGACGT TTGACTCCTC AGGGGCCTTC CTCTCACTTA AGGTGAGGGC GCTGGAAAAA 
GCTCCTAAGG AGCCAATCCT GGAAGAGGCG GAGCTGGACT TCCGGCCATT GGAGGAGAAT 
GACGAATATG CAGAAAAGGA TGACAGTGAA ACTGAACAAA CCAAAGATAC TGACACAAAT 
ACTAGGCACG AAAGTGATCG AAATGAAGAA AACTGTAAAT CAGAAGAGCC GTCGCCAGTT 
GCGGTACCAT TTTCAGCAGT AGTTACGCCT CCCAAAGCCA CCACCCCTGC ACCCATTCAG 
CCTGTACACC TAGAAAAAGC TGAAGATAAG GAGAGACCGT CAGAGAGAAC AACATTACCA 
GAAATCAGAC ATGAACTGAG TAAAGCACCA CTACACACAG CCCTATCTAA CAGCATTGTG 
GAAGCCATTT CCATACCGCA TGTCGCAAAC AAACTCCCAG ATCTTCCGGT CCCAGCGCCT 
TCAGTTCTGC CTGTCAAGTC AGTGCCCCCT CAATCCCAAC AGGTCAAGCC TATAGAGATG 
CCAGTGTCAG TTCCTCCTGC TCTGCTACGC TCCACAGGCA GTGTGGGTCC TATCAGTCGA 
CCCTCTGCCC TGCCATCGGA CTTGGATGAG GATGAGGGAC TCAAGCACTT TGAGCAGGAA 
GCTGAGAAAA TGGTGGCGTA CCTCCAGGAT GGTGGCGTAG ATGATGAACG TTTGGCCTCT 
AAAATTGGAC CCAAACCATC AGCTCTTCCA ATCACAAATG AAGCTGCATT CAAATGGTTT 
TACAAAGATC CGCAGGGGGA AATCCAAGGA CCATTTAATA ATCAGGAGAT GTCTGAGTGG 
TTTCAGGCGG GCTATTTCAC CATGACCTTG CAGGTAAAGC GAGGATGCGA CGAGATGTTC 
CAGCCTCTGG GCGAGATGAT TAAGTTGTGG GGACGAGTGC CCTTTACACC GGGCCCGACA 
CTACCTCCCA TACTGGGAGA TGCAGATCAA GAAAGGATGA AGAGACAACA GGAGATTAAT 
GCTCTAAATA TGTATCAGCT ACAGCAGATG CAATATCAGT ATTTACTCAG GCAACAATAT 
GCTCTGCAGC AGAAAGTGTT AAACTCTGCT CCTCCTCCTC CTCCACCACC ACAGCAGCAG 
CTCAATCTTC TTCTCCATCA GGCGCTGAAG ATCAGAACTC CTGAGCCGCA GCAGAGCCTC 
TTACCTCCCG TCACACGCTC CATGTCGGTT CCAGACTCGG GTTCTGTTTG GGAAATGCAG 
AACCCGTCCA CTCAGGCATC CTGTTCTCCA AACATGCAGC CCGCCGCTCC CAGCACTTGG 
GAAGGGAGTA GCGTATGGGA TCTGCCACTA GACAATATGC CTCAAGCGTC TTCCATTGAA 
CAGCTGCAGC TGGAGAAAGC CAAAGCTTTG AAGCTGGAGA TGGAGAGACG GGAGGCAGAG 
CTTCGGGCCA AAAGGGAAGA AGAGGAGAGG AAGCGTTTGG AAGAGGCTCT GCGTGCGAGA 
CAAGAGGAAG AGAGGAAGCG TTTAGAGGAA GAAGAGCTGG CACGACGCAA GCAGGAGGAG 
GCTCTGAAGA GACAGCGGGA GCAGGAGGAG GCGCAGCGGA GGAAAAAGGA AGAGGAGGAA 
CGGTTAGCTC AGGAGGAGGC TCTCCGGCGA CTGGAGGAGC GGAGGAGAGA AGAAGAGGAG 
AGGAGACAAA GGGAAGAATT CCTCCGCAAA CAGGAAGAGG AGCGGAGGAA GCAGGAAGAA 
CTGGAAGCTC AGAGGCGGCG TGAGGAAGAG AAGAGGCTGG AGGAGGAGGC TGCGGCCGCT 
GCAGCAGCAC TGCTCAGACA ACAGCAGGAG GAGCAGAAGA AGAGAGAGCA GGAGGCGCAG 
AGACAGCAGG AGCTGCAGAG ACAGAGACAG CAGCAACAGG AGGCACTCCG ACGACTCCAG 
CAGCAGCAGC AACAACAGCA GCTCGCACAA ATGAAGCTAC CTTCATCCTC AAAGTGGGGT 
CAGCAGTCGA CCACAGCAAA CAGTCTCTCA CAGTCTCAAA ATGCTCTTTC GCTCGCTGAG 
ATCCAGAAAC TGGAGGAGGA GAGAGAACGA CAGACTCGAG AAGAGCAGAG ACGTCAGCAG 
CAGGAGCTCC AGCGGGTCCA GCAGCAGCAG CCTCAGACCA AGCTCCCTGG CTGGGGCAGT 
GTGGCTAAGC AGCCCATGGC CACTAAATCC CTGTTGGAGA TCCAGAGAGA GGAAGCACAG 
CAGATGAAGC AGCGGAAAGA TCAGCAGCAG CAGCAACAAC AACAACAACA ACCACCTCCA 
CAGCCTCAGC CTCAGCAACA TTCAACCAAC ACCCAGCAAA ATCGCACACA AAACCGGGCG 
GCCATCAATA CGTCAGTGTG GGGTTCTGTC AATAATGTGA GCTCAAACTG GATGATGGAC 
AGCAGTGTAT GGGGCGACAC GCAGAACTCT AATATTGGCT TCTGGGATGA AGCAGTAAAG 
GAGGCCGCTC CACCTCAAAC CACTCGCAAG AGCCACACTC CGAAAAACAA GGGCAACGCT 
AACCTCAGTA ATAGCAGCAG CGGTAAAGCC AGTAAAAAGG TGGAAGAGGA GGAGAAGCTA 
CTGAAACTGT TCCAGGGAGC CAATAAGAAC CAGGACGGCT TTATGCAGTG GTGTGAACAG 
ACCCTGCACA CTCTCAACAC AGCCAATAAC CTCGATGTTC CCACTTTTGC ATCGTTCCTG 
AAGGAAGTGG ACTCTCCGTA TGAGGTGCAC GATTATGTAC GAGCTTATCT GGGTGACACT 
CCACAGGCCA AGGACTTCGC CAAGCAGTTC CTGGAGCGCC GTGCCAAACA GAACGACAAC 
CAGCAGAAAC CTCAGCAGGG CCAGCAACAA AAACAGCAGG AGTCGGTGTG GGGAATAAGA 
GAAGTGCCAC AGTCAGTCCT GCTTCAACAA CAACAACAAC AACAATTACA GCAGCAGCAA 
CAGCAGCGGT TTGAAACGGT CACTTCCGGC AAGAAGAAGA AGAAACAGAA GATGGTACGA 
GCCGACCCAA GCCTTCTAGG TTTTTCTGTC AATGCCTCCT CTGAGAGACT CAATATGGGC 
GAGATCGAGA CGGTTGAAGA CTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214142.1
CDS	102..4088
Translation	MAETQTLNFGPEWLRALAGGVGSSIVASPPLSPALPKYKLADYRYGREEMLALYVKDNMIPVDLHDKEFLPILQEEPLPPLALVPFTEEEQRNFSMSVNSAAVLRLTGRGGGTVAGAPRGRSSSRGRGRGRGDGFYQRSFDDVEGGFGRGGREMHRSQSWEERGDRRFEKPGRKDPDGAPAHFPLNHIRANYEDPAAVNARKFIHAECDNWRTTRDELNGEEDDNGWRLAGARRENERWCPPSPDGPRSAGWREHPDQRRRFGFGDEERSGYRRPRSGSGSAEEERDSLPEWCLEDAEEETGTFDSSGAFLSLKVRALEKAPKEPILEEAELDFRPLEENDEYAEKDDSETEQTKDTDTNTRHESDRNEENCKSEEPSPVAVPFSAVVTPPKATTPAPIQPVHLEKAEDKERPSERTTLPEIRHELSKAPLHTALSNSIVEAISIPHVANKLPDLPVPAPSVLPVKSVPPQSQQVKPIEMPVSVPPALLRSTGSVGPISRPSALPSDLDEDEGLKHFEQEAEKMVAYLQDGGVDDERLASKIGPKPSALPITNEAAFKWFYKDPQGEIQGPFNNQEMSEWFQAGYFTMTLQVKRGCDEMFQPLGEMIKLWGRVPFTPGPTLPPILGDADQERMKRQQEINALNMYQLQQMQYQYLLRQQYALQQKVLNSAPPPPPPPQQQLNLLLHQALKIRTPEPQQSLLPPVTRSMSVPDSGSVWEMQNPSTQASCSPNMQPAAPSTWEGSSVWDLPLDNMPQASSIEQLQLEKAKALKLEMERREAELRAKREEEERKRLEEALRARQEEERKRLEEEELARRKQEEALKRQREQEEAQRRKKEEEERLAQEEALRRLEERRREEEERRQREEFLRKQEEERRKQEELEAQRRREEEKRLEEEAAAAAAALLRQQQEEQKKREQEAQRQQELQRQRQQQQEALRRLQQQQQQQQLAQMKLPSSSKWGQQSTTANSLSQSQNALSLAEIQKLEEERERQTREEQRRQQQELQRVQQQQPQTKLPGWGSVAKQPMATKSLLEIQREEAQQMKQRKDQQQQQQQQQQPPPQPQPQQHSTNTQQNRTQNRAAINTSVWGSVNNVSSNWMMDSSVWGDTQNSNIGFWDEAVKEAAPPQTTRKSHTPKNKGNANLSNSSSGKASKKVEEEEKLLKLFQGANKNQDGFMQWCEQTLHTLNTANNLDVPTFASFLKEVDSPYEVHDYVRAYLGDTPQAKDFAKQFLERRAKQNDNQQKPQQGQQQKQQESVWGIREVPQSVLLQQQQQQQLQQQQQQRFETVTSGKKKKKQKMVRADPSLLGFSVNASSERLNMGEIETVEDF

Target ORF information:

RefSeq Version	XM_017358653.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio GRB10 interacting GYF protein 2 (gigfy2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358653.1

ORF Insert Sequence:

ATGGCGGAAA CCCAGACACT CAACTTTGGG CCAGAATGGC TCCGTGCTCT GGCTGGTGGG 
GTGGGCAGCA GCATTGTGGC CTCCCCTCCG CTCTCGCCTG CATTGCCAAA GTATAAACTT 
GCAGACTATC GCTACGGGAG AGAAGAGATG CTAGCACTTT ATGTAAAGGA TAACATGATT 
CCGGTAGATC TTCATGATAA GGAATTCCTA CCTATTTTAC AAGAGGAGCC CCTGCCACCT 
TTGGCATTAG TCCCTTTTAC AGAGGAGGAA CAGAGAAATT TCTCCATGTC TGTAAACAGT 
GCAGCAGTTC TCCGTCTGAC AGGAAGGGGG GGAGGGACGG TGGCAGGGGC ACCTAGAGGC 
CGAAGTTCTT CACGAGGGCG AGGGAGAGGC AGAGGAGATG GTTTTTACCA AAGAAGTTTT 
GATGATGTGG AAGGAGGTTT CGGTCGTGGA GGGAGAGAGA TGCATCGCTC CCAGAGTTGG 
GAGGAGAGGG GAGACAGAAG GTTTGAAAAG CCAGGTCGAA AAGATCCAGA TGGAGCACCA 
GCCCATTTTC CACTCAATCA CATAAGGGCA AACTATGAGG ACCCTGCTGC AGTGAATGCC 
CGAAAGTTCA TTCATGCAGA GTGTGACAAC TGGCGCACCA CTCGAGATGA GCTGAATGGA 
GAGGAGGATG ATAACGGATG GAGGCTTGCA GGCGCTCGTC GGGAAAATGA GCGATGGTGT 
CCTCCAAGTC CAGATGGTCC ACGATCAGCA GGGTGGCGGG AACACCCAGA TCAACGTCGA 
CGCTTTGGTT TTGGGGATGA GGAACGCAGT GGTTACCGTA GGCCTCGGTC GGGCAGCGGG 
AGCGCAGAGG AGGAGAGGGA CAGTTTACCA GAATGGTGTC TGGAAGATGC AGAGGAGGAG 
ACAGGGACGT TTGACTCCTC AGGGGCCTTC CTCTCACTTA AGGTGAGGGC GCTGGAAAAA 
GCTCCTAAGG AGCCAATCCT GGAAGAGGCG GAGCTGGACT TCCGGCCATT GGAGGAGAAT 
GACGAATATG CAGAAAAGGA TGACAGTGAA ACTGAACAAA CCAAAGATAC TGACACAAAT 
ACTAGGCACG AAAGTGATCG AAATGAAGAA AACTGTAAAT CAGAAGAGCC GTCGCCAGTT 
GCGGTACCAT TTTCAGCAGT AGTTACGCCT CCCAAAGCCA CCACCCCTGC ACCCATTCAG 
CCTGTACACC TAGAAAAAGC TGAAGATAAG GAGAGACCGT CAGAGAGAAC AACATTACCA 
GAAATCAGAC ATGAACTGAG TAAAGCACCA CTACACACAG CCCTATCTAA CAGCATTGTG 
GAAGCCATTT CCATACCGCA TGTCGCAAAC AAACTCCCAG ATCTTCCGGT CCCAGCGCCT 
TCAGTTCTGC CTGTCAAGTC AGTGCCCCCT CAATCCCAAC AGGTCAAGCC TATAGAGATG 
CCAGTGTCAG TTCCTCCTGC TCTGCTACGC TCCACAGGCA GTGTGGGTCC TATCAGTCGA 
CCCTCTGCCC TGCCATCGGA CTTGGATGAG GATGAGGGAC TCAAGCACTT TGAGCAGGAA 
GCTGAGAAAA TGGTGGCGTA CCTCCAGGAT GGTGGCGTAG ATGATGAACG TTTGGCCTCT 
AAAATTGGAC CCAAACCATC AGCTCTTCCA ATCACAAATG AAGCTGCATT CAAATGGTTT 
TACAAAGATC CGCAGGGGGA AATCCAAGGA CCATTTAATA ATCAGGAGAT GTCTGAGTGG 
TTTCAGGCGG GCTATTTCAC CATGACCTTG CAGGTAAAGC GAGGATGCGA CGAGATGTTC 
CAGCCTCTGG GCGAGATGAT TAAGTTGTGG GGACGAGTGC CCTTTACACC GGGCCCGACA 
CTACCTCCCA TACTGGGAGA TGCAGATCAA GAAAGGATGA AGAGACAACA GGAGATTAAT 
GCTCTAAATA TGTATCAGCT ACAGCAGATG CAATATCAGT ATTTACTCAG GCAACAATAT 
GCTCTGCAGC AGAAAGTGTT AAACTCTGCT CCTCCTCCTC CTCCACCACC ACAGCAGCAG 
CTCAATCTTC TTCTCCATCA GGCGCTGAAG ATCAGAACTC CTGAGCCGCA GCAGAGCCTC 
TTACCTCCCG TCACACGCTC CATGTCGGTT CCAGACTCGG GTTCTGTTTG GGAAATGCAG 
AACCCGTCCA CTCAGGCATC CTGTTCTCCA AACATGCAGC CCGCCGCTCC CAGCACTTGG 
GAAGGGAGTA GCGTATGGGA TCTGCCACTA GACAATATGC CTCAAGCGTC TTCCATTGAA 
CAGCTGCAGC TGGAGAAAGC CAAAGCTTTG AAGCTGGAGA TGGAGAGACG GGAGGCAGAG 
CTTCGGGCCA AAAGGGAAGA AGAGGAGAGG AAGCGTTTGG AAGAGGCTCT GCGTGCGAGA 
CAAGAGGAAG AGAGGAAGCG TTTAGAGGAA GAAGAGCTGG CACGACGCAA GCAGGAGGAG 
GCTCTGAAGA GACAGCGGGA GCAGGAGGAG GCGCAGCGGA GGAAAAAGGA AGAGGAGGAA 
CGGTTAGCTC AGGAGGAGGC TCTCCGGCGA CTGGAGGAGC GGAGGAGAGA AGAAGAGGAG 
AGGAGACAAA GGGAAGAATT CCTCCGCAAA CAGGAAGAGG AGCGGAGGAA GCAGGAAGAA 
CTGGAAGCTC AGAGGCGGCG TGAGGAAGAG AAGAGGCTGG AGGAGGAGGC TGCGGCCGCT 
GCAGCAGCAC TGCTCAGACA ACAGCAGGAG GAGCAGAAGA AGAGAGAGCA GGAGGCGCAG 
AGACAGCAGG AGCTGCAGAG ACAGAGACAG CAGCAACAGG AGGCACTCCG ACGACTCCAG 
CAGCAGCAGC AACAACAGCA GCTCGCACAA ATGAAGCTAC CTTCATCCTC AAAGTGGGGT 
CAGCAGTCGA CCACAGCAAA CAGTCTCTCA CAGTCTCAAA ATGCTCTTTC GCTCGCTGAG 
ATCCAGAAAC TGGAGGAGGA GAGAGAACGA CAGACTCGAG AAGAGCAGAG ACGTCAGCAG 
CAGGAGCTCC AGCGGGTCCA GCAGCAGCAG CCTCAGACCA AGCTCCCTGG CTGGGGCAGT 
GTGGCTAAGC AGCCCATGGC CACTAAATCC CTGTTGGAGA TCCAGAGAGA GGAAGCACAG 
CAGATGAAGC AGCGGAAAGA TCAGCAGCAG CAGCAACAAC AACAACAACA ACCACCTCCA 
CAGCCTCAGC CTCAGCAACA TTCAACCAAC ACCCAGCAAA ATCGCACACA AAACCGGGCG 
GCCATCAATA CGTCAGTGTG GGGTTCTGTC AATAATGTGA GCTCAAACTG GATGATGGAC 
AGCAGTGTAT GGGGCGACAC GCAGAACTCT AATATTGGCT TCTGGGATGA AGCAGTAAAG 
GAGGCCGCTC CACCTCAAAC CACTCGCAAG AGCCACACTC CGAAAAACAA GGGCAACGCT 
AACCTCAGTA ATAGCAGCAG CGGTAAAGCC AGTAAAAAGG TGGAAGAGGA GGAGAAGCTA 
CTGAAACTGT TCCAGGGAGC CAATAAGAAC CAGGACGGCT TTATGCAGTG GTGTGAACAG 
ACCCTGCACA CTCTCAACAC AGCCAATAAC CTCGATGTTC CCACTTTTGC ATCGTTCCTG 
AAGGAAGTGG ACTCTCCGTA TGAGGTGCAC GATTATGTAC GAGCTTATCT GGGTGACACT 
CCACAGGCCA AGGACTTCGC CAAGCAGTTC CTGGAGCGCC GTGCCAAACA GAACGACAAC 
CAGCAGAAAC CTCAGCAGGG CCAGCAACAA AAACAGCAGG AGTCGGTGTG GGGAATAAGA 
GAAGTGCCAC AGTCAGTCCT GCTTCAACAA CAACAACAAC AACAATTACA GCAGCAGCAA 
CAGCAGCGGT TTGAAACGGT CACTTCCGGC AAGAAGAAGA AGAAACAGAA GATGGTACGA 
GCCGACCCAA GCCTTCTAGG TTTTTCTGTC AATGCCTCCT CTGAGAGACT CAATATGGGC 
GAGATCGAGA CGGTTGAAGA CTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gigfy2 ( XM_017358654.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214143.1 Danio rerio GRB10 interacting GYF protein 2 (gigfy2), transcript variant X2, mRNA.

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CloneID	ODa56296
Clone ID Related Accession (Same CDS sequence)	XM_017358654.1 , XM_017358654.2
Accession Version	XM_017358654.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	PERQ amino acid-rich with GYF domain-containing protein 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAAA CCCAGACACT CAACTTTGGG CCAGAATGGC TCCGTGCTCT GGCTGGTGGG 
GTGGGCAGCA GCATTGTGGC CTCCCCTCCG CTCTCGCCTG CATTGCCAAA GTATAAACTT 
GCAGACTATC GCTACGGGAG AGAAGAGATG CTAGCACTTT ATGTAAAGGA TAACATGATT 
CCGGTAGATC TTCATGATAA GGAATTCCTA CCTATTTTAC AAGAGGAGCC CCTGCCACCT 
TTGGCATTAG TCCCTTTTAC AGAGGAGGAA CAGAGAAATT TCTCCATGTC TGTAAACAGT 
GCAGCAGTTC TCCGTCTGAC AGGAAGGGGG GGAGGGACGG TGGCAGGGGC ACCTAGAGGC 
CGAAGTTCTT CACGAGGGCG AGGGAGAGGC AGAGGAGATG GTTTTTACCA AAGAAGTTTT 
GATGATGTGG AAGGAGGTTT CGGTCGTGGA GGGAGAGAGA TGCATCGCTC CCAGAGTTGG 
GAGGAGAGGG GAGACAGAAG GTTTGAAAAG CCAGGTCGAA AAGATCCAGA TGGAGCACCA 
GCCCATTTTC CACTCAATCA CATAAGGGCA AACTATGAGG ACCCTGCTGC AGTGAATGCC 
CGAAAGTTCA TTCATGCAGA GTGTGACAAC TGGCGCACCA CTCGAGATGA GCTGAATGGA 
GAGGAGGATG ATAACGGATG GAGGCTTGCA GGCGCTCGTC GGGAAAATGA GCGATGGTGT 
CCTCCAAGTC CAGATGGTCC ACGATCAGCA GGGTGGCGGG AACACCCAGA TCAACGTCGA 
CGCTTTGGTT TTGGGGATGA GGAACGCAGT GGTTACCGTA GGCCTCGGTC GGGCAGCGGG 
AGCGCAGAGG AGGAGAGGGA CAGTTTACCA GAATGGTGTC TGGAAGATGC AGAGGAGGAG 
ACAGGGACGT TTGACTCCTC AGGGGCCTTC CTCTCACTTA AGAAAGCTCC TAAGGAGCCA 
ATCCTGGAAG AGGCGGAGCT GGACTTCCGG CCATTGGAGG AGAATGACGA ATATGCAGAA 
AAGGATGACA GTGAAACTGA ACAAACCAAA GATACTGACA CAAATACTAG GCACGAAAGT 
GATCGAAATG AAGAAAACTG TAAATCAGAA GAGCCGTCGC CAGTTGCGGT ACCATTTTCA 
GCAGTAGTTA CGCCTCCCAA AGCCACCACC CCTGCACCCA TTCAGCCTGT ACACCTAGAA 
AAAGCTGAAG ATAAGGAGAG ACCGTCAGAG AGAACAACAT TACCAGAAAT CAGACATGAA 
CTGAGTAAAG CACCACTACA CACAGCCCTA TCTAACAGCA TTGTGGAAGC CATTTCCATA 
CCGCATGTCG CAAACAAACT CCCAGATCTT CCGGTCCCAG CGCCTTCAGT TCTGCCTGTC 
AAGTCAGTGC CCCCTCAATC CCAACAGGTC AAGCCTATAG AGATGCCAGT GTCAGTTCCT 
CCTGCTCTGC TACGCTCCAC AGGCAGTGTG GGTCCTATCA GTCGACCCTC TGCCCTGCCA 
TCGGACTTGG ATGAGGATGA GGGACTCAAG CACTTTGAGC AGGAAGCTGA GAAAATGGTG 
GCGTACCTCC AGGATGGTGG CGTAGATGAT GAACGTTTGG CCTCTAAAAT TGGACCCAAA 
CCATCAGCTC TTCCAATCAC AAATGAAGCT GCATTCAAAT GGTTTTACAA AGATCCGCAG 
GGGGAAATCC AAGGACCATT TAATAATCAG GAGATGTCTG AGTGGTTTCA GGCGGGCTAT 
TTCACCATGA CCTTGCAGGT AAAGCGAGGA TGCGACGAGA TGTTCCAGCC TCTGGGCGAG 
ATGATTAAGT TGTGGGGACG AGTGCCCTTT ACACCGGGCC CGACACTACC TCCCATACTG 
GGAGATGCAG ATCAAGAAAG GATGAAGAGA CAACAGGAGA TTAATGCTCT AAATATGTAT 
CAGCTACAGC AGATGCAATA TCAGTATTTA CTCAGGCAAC AATATGCTCT GCAGCAGAAA 
GTGTTAAACT CTGCTCCTCC TCCTCCTCCA CCACCACAGC AGCAGCTCAA TCTTCTTCTC 
CATCAGGCGC TGAAGATCAG AACTCCTGAG CCGCAGCAGA GCCTCTTACC TCCCGTCACA 
CGCTCCATGT CGGTTCCAGA CTCGGGTTCT GTTTGGGAAA TGCAGAACCC GTCCACTCAG 
GCATCCTGTT CTCCAAACAT GCAGCCCGCC GCTCCCAGCA CTTGGGAAGG GAGTAGCGTA 
TGGGATCTGC CACTAGACAA TATGCCTCAA GCGTCTTCCA TTGAACAGCT GCAGCTGGAG 
AAAGCCAAAG CTTTGAAGCT GGAGATGGAG AGACGGGAGG CAGAGCTTCG GGCCAAAAGG 
GAAGAAGAGG AGAGGAAGCG TTTGGAAGAG GCTCTGCGTG CGAGACAAGA GGAAGAGAGG 
AAGCGTTTAG AGGAAGAAGA GCTGGCACGA CGCAAGCAGG AGGAGGCTCT GAAGAGACAG 
CGGGAGCAGG AGGAGGCGCA GCGGAGGAAA AAGGAAGAGG AGGAACGGTT AGCTCAGGAG 
GAGGCTCTCC GGCGACTGGA GGAGCGGAGG AGAGAAGAAG AGGAGAGGAG ACAAAGGGAA 
GAATTCCTCC GCAAACAGCA GGAAGAGGAG CGGAGGAAGC AGGAAGAACT GGAAGCTCAG 
AGGCGGCGTG AGGAAGAGAA GAGGCTGGAG GAGGAGGCTG CGGCCGCTGC AGCAGCACTG 
CTCAGACAAC AGCAGGAGGA GCAGAAGAAG AGAGAGCAGG AGGCGCAGAG ACAGCAGGAG 
CTGCAGAGAC AGAGACAGCA GCAACAGGAG GCACTCCGAC GACTCCAGCA GCAGCAGCAA 
CAACAGCAGC TCGCACAAAT GAAGCTACCT TCATCCTCAA AGTGGGGTCA GCAGTCGACC 
ACAGCAAACA GTCTCTCACA GTCTCAAAAT GCTCTTTCGC TCGCTGAGAT CCAGAAACTG 
GAGGAGGAGA GAGAACGACA GACTCGAGAA GAGCAGAGAC GTCAGCAGCA GGAGCTCCAG 
CGGGTCCAGC AGCAGCAGCC TCAGACCAAG CTCCCTGGCT GGGGCAGTGT GGCTAAGCAG 
CCCATGGCCA CTAAATCCCT GTTGGAGATC CAGAGAGAGG AAGCACAGCA GATGAAGCAG 
CGGAAAGATC AGCAGCAGCA GCAACAACAA CAACAACAAC CACCTCCACA GCCTCAGCCT 
CAGCAACATT CAACCAACAC CCAGCAAAAT CGCACACAAA ACCGGGCGGC CATCAATACG 
TCAGTGTGGG GTTCTGTCAA TAATGTGAGC TCAAACTGGA TGATGGACAG CAGTGTATGG 
GGCGACACGC AGAACTCTAA TATTGGCTTC TGGGATGAAG CAGTAAAGGA GGCCGCTCCA 
CCTCAAACCA CTCGCAAGAG CCACACTCCG AAAAACAAGG GCAACGCTAA CCTCAGTAAT 
AGCAGCAGCG GTAAAGCCAG TAAAAAGGTG GAAGAGGAGG AGAAGCTACT GAAACTGTTC 
CAGGGAGCCA ATAAGAACCA GGACGGCTTT ATGCAGTGGT GTGAACAGAC CCTGCACACT 
CTCAACACAG CCAATAACCT CGATGTTCCC ACTTTTGCAT CGTTCCTGAA GGAAGTGGAC 
TCTCCGTATG AGGTGCACGA TTATGTACGA GCTTATCTGG GTGACACTCC ACAGGCCAAG 
GACTTCGCCA AGCAGTTCCT GGAGCGCCGT GCCAAACAGA ACGACAACCA GCAGAAACCT 
CAGCAGGGCC AGCAACAAAA ACAGCAGGAG TCGGTGTGGG GAATAAGAGA AGTGCCACAG 
TCAGTCCTGC TTCAACAACA ACAACAACAA CAATTACAGC AGCAGCAACA GCAGCGGTTT 
GAAACGGTCA CTTCCGGCAA GAAGAAGAAG AAACAGAAGA TGGTACGAGC CGACCCAAGC 
CTTCTAGGTT TTTCTGTCAA TGCCTCCTCT GAGAGACTCA ATATGGGCGA GATCGAGACG 
GTTGAAGACT TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214143.1
CDS	102..4076
Translation	MAETQTLNFGPEWLRALAGGVGSSIVASPPLSPALPKYKLADYRYGREEMLALYVKDNMIPVDLHDKEFLPILQEEPLPPLALVPFTEEEQRNFSMSVNSAAVLRLTGRGGGTVAGAPRGRSSSRGRGRGRGDGFYQRSFDDVEGGFGRGGREMHRSQSWEERGDRRFEKPGRKDPDGAPAHFPLNHIRANYEDPAAVNARKFIHAECDNWRTTRDELNGEEDDNGWRLAGARRENERWCPPSPDGPRSAGWREHPDQRRRFGFGDEERSGYRRPRSGSGSAEEERDSLPEWCLEDAEEETGTFDSSGAFLSLKKAPKEPILEEAELDFRPLEENDEYAEKDDSETEQTKDTDTNTRHESDRNEENCKSEEPSPVAVPFSAVVTPPKATTPAPIQPVHLEKAEDKERPSERTTLPEIRHELSKAPLHTALSNSIVEAISIPHVANKLPDLPVPAPSVLPVKSVPPQSQQVKPIEMPVSVPPALLRSTGSVGPISRPSALPSDLDEDEGLKHFEQEAEKMVAYLQDGGVDDERLASKIGPKPSALPITNEAAFKWFYKDPQGEIQGPFNNQEMSEWFQAGYFTMTLQVKRGCDEMFQPLGEMIKLWGRVPFTPGPTLPPILGDADQERMKRQQEINALNMYQLQQMQYQYLLRQQYALQQKVLNSAPPPPPPPQQQLNLLLHQALKIRTPEPQQSLLPPVTRSMSVPDSGSVWEMQNPSTQASCSPNMQPAAPSTWEGSSVWDLPLDNMPQASSIEQLQLEKAKALKLEMERREAELRAKREEEERKRLEEALRARQEEERKRLEEEELARRKQEEALKRQREQEEAQRRKKEEEERLAQEEALRRLEERRREEEERRQREEFLRKQQEEERRKQEELEAQRRREEEKRLEEEAAAAAAALLRQQQEEQKKREQEAQRQQELQRQRQQQQEALRRLQQQQQQQQLAQMKLPSSSKWGQQSTTANSLSQSQNALSLAEIQKLEEERERQTREEQRRQQQELQRVQQQQPQTKLPGWGSVAKQPMATKSLLEIQREEAQQMKQRKDQQQQQQQQQQPPPQPQPQQHSTNTQQNRTQNRAAINTSVWGSVNNVSSNWMMDSSVWGDTQNSNIGFWDEAVKEAAPPQTTRKSHTPKNKGNANLSNSSSGKASKKVEEEEKLLKLFQGANKNQDGFMQWCEQTLHTLNTANNLDVPTFASFLKEVDSPYEVHDYVRAYLGDTPQAKDFAKQFLERRAKQNDNQQKPQQGQQQKQQESVWGIREVPQSVLLQQQQQQQLQQQQQQRFETVTSGKKKKKQKMVRADPSLLGFSVNASSERLNMGEIETVEDF

Target ORF information:

RefSeq Version	XM_017358654.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio GRB10 interacting GYF protein 2 (gigfy2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358654.1

ORF Insert Sequence:

ATGGCGGAAA CCCAGACACT CAACTTTGGG CCAGAATGGC TCCGTGCTCT GGCTGGTGGG 
GTGGGCAGCA GCATTGTGGC CTCCCCTCCG CTCTCGCCTG CATTGCCAAA GTATAAACTT 
GCAGACTATC GCTACGGGAG AGAAGAGATG CTAGCACTTT ATGTAAAGGA TAACATGATT 
CCGGTAGATC TTCATGATAA GGAATTCCTA CCTATTTTAC AAGAGGAGCC CCTGCCACCT 
TTGGCATTAG TCCCTTTTAC AGAGGAGGAA CAGAGAAATT TCTCCATGTC TGTAAACAGT 
GCAGCAGTTC TCCGTCTGAC AGGAAGGGGG GGAGGGACGG TGGCAGGGGC ACCTAGAGGC 
CGAAGTTCTT CACGAGGGCG AGGGAGAGGC AGAGGAGATG GTTTTTACCA AAGAAGTTTT 
GATGATGTGG AAGGAGGTTT CGGTCGTGGA GGGAGAGAGA TGCATCGCTC CCAGAGTTGG 
GAGGAGAGGG GAGACAGAAG GTTTGAAAAG CCAGGTCGAA AAGATCCAGA TGGAGCACCA 
GCCCATTTTC CACTCAATCA CATAAGGGCA AACTATGAGG ACCCTGCTGC AGTGAATGCC 
CGAAAGTTCA TTCATGCAGA GTGTGACAAC TGGCGCACCA CTCGAGATGA GCTGAATGGA 
GAGGAGGATG ATAACGGATG GAGGCTTGCA GGCGCTCGTC GGGAAAATGA GCGATGGTGT 
CCTCCAAGTC CAGATGGTCC ACGATCAGCA GGGTGGCGGG AACACCCAGA TCAACGTCGA 
CGCTTTGGTT TTGGGGATGA GGAACGCAGT GGTTACCGTA GGCCTCGGTC GGGCAGCGGG 
AGCGCAGAGG AGGAGAGGGA CAGTTTACCA GAATGGTGTC TGGAAGATGC AGAGGAGGAG 
ACAGGGACGT TTGACTCCTC AGGGGCCTTC CTCTCACTTA AGAAAGCTCC TAAGGAGCCA 
ATCCTGGAAG AGGCGGAGCT GGACTTCCGG CCATTGGAGG AGAATGACGA ATATGCAGAA 
AAGGATGACA GTGAAACTGA ACAAACCAAA GATACTGACA CAAATACTAG GCACGAAAGT 
GATCGAAATG AAGAAAACTG TAAATCAGAA GAGCCGTCGC CAGTTGCGGT ACCATTTTCA 
GCAGTAGTTA CGCCTCCCAA AGCCACCACC CCTGCACCCA TTCAGCCTGT ACACCTAGAA 
AAAGCTGAAG ATAAGGAGAG ACCGTCAGAG AGAACAACAT TACCAGAAAT CAGACATGAA 
CTGAGTAAAG CACCACTACA CACAGCCCTA TCTAACAGCA TTGTGGAAGC CATTTCCATA 
CCGCATGTCG CAAACAAACT CCCAGATCTT CCGGTCCCAG CGCCTTCAGT TCTGCCTGTC 
AAGTCAGTGC CCCCTCAATC CCAACAGGTC AAGCCTATAG AGATGCCAGT GTCAGTTCCT 
CCTGCTCTGC TACGCTCCAC AGGCAGTGTG GGTCCTATCA GTCGACCCTC TGCCCTGCCA 
TCGGACTTGG ATGAGGATGA GGGACTCAAG CACTTTGAGC AGGAAGCTGA GAAAATGGTG 
GCGTACCTCC AGGATGGTGG CGTAGATGAT GAACGTTTGG CCTCTAAAAT TGGACCCAAA 
CCATCAGCTC TTCCAATCAC AAATGAAGCT GCATTCAAAT GGTTTTACAA AGATCCGCAG 
GGGGAAATCC AAGGACCATT TAATAATCAG GAGATGTCTG AGTGGTTTCA GGCGGGCTAT 
TTCACCATGA CCTTGCAGGT AAAGCGAGGA TGCGACGAGA TGTTCCAGCC TCTGGGCGAG 
ATGATTAAGT TGTGGGGACG AGTGCCCTTT ACACCGGGCC CGACACTACC TCCCATACTG 
GGAGATGCAG ATCAAGAAAG GATGAAGAGA CAACAGGAGA TTAATGCTCT AAATATGTAT 
CAGCTACAGC AGATGCAATA TCAGTATTTA CTCAGGCAAC AATATGCTCT GCAGCAGAAA 
GTGTTAAACT CTGCTCCTCC TCCTCCTCCA CCACCACAGC AGCAGCTCAA TCTTCTTCTC 
CATCAGGCGC TGAAGATCAG AACTCCTGAG CCGCAGCAGA GCCTCTTACC TCCCGTCACA 
CGCTCCATGT CGGTTCCAGA CTCGGGTTCT GTTTGGGAAA TGCAGAACCC GTCCACTCAG 
GCATCCTGTT CTCCAAACAT GCAGCCCGCC GCTCCCAGCA CTTGGGAAGG GAGTAGCGTA 
TGGGATCTGC CACTAGACAA TATGCCTCAA GCGTCTTCCA TTGAACAGCT GCAGCTGGAG 
AAAGCCAAAG CTTTGAAGCT GGAGATGGAG AGACGGGAGG CAGAGCTTCG GGCCAAAAGG 
GAAGAAGAGG AGAGGAAGCG TTTGGAAGAG GCTCTGCGTG CGAGACAAGA GGAAGAGAGG 
AAGCGTTTAG AGGAAGAAGA GCTGGCACGA CGCAAGCAGG AGGAGGCTCT GAAGAGACAG 
CGGGAGCAGG AGGAGGCGCA GCGGAGGAAA AAGGAAGAGG AGGAACGGTT AGCTCAGGAG 
GAGGCTCTCC GGCGACTGGA GGAGCGGAGG AGAGAAGAAG AGGAGAGGAG ACAAAGGGAA 
GAATTCCTCC GCAAACAGCA GGAAGAGGAG CGGAGGAAGC AGGAAGAACT GGAAGCTCAG 
AGGCGGCGTG AGGAAGAGAA GAGGCTGGAG GAGGAGGCTG CGGCCGCTGC AGCAGCACTG 
CTCAGACAAC AGCAGGAGGA GCAGAAGAAG AGAGAGCAGG AGGCGCAGAG ACAGCAGGAG 
CTGCAGAGAC AGAGACAGCA GCAACAGGAG GCACTCCGAC GACTCCAGCA GCAGCAGCAA 
CAACAGCAGC TCGCACAAAT GAAGCTACCT TCATCCTCAA AGTGGGGTCA GCAGTCGACC 
ACAGCAAACA GTCTCTCACA GTCTCAAAAT GCTCTTTCGC TCGCTGAGAT CCAGAAACTG 
GAGGAGGAGA GAGAACGACA GACTCGAGAA GAGCAGAGAC GTCAGCAGCA GGAGCTCCAG 
CGGGTCCAGC AGCAGCAGCC TCAGACCAAG CTCCCTGGCT GGGGCAGTGT GGCTAAGCAG 
CCCATGGCCA CTAAATCCCT GTTGGAGATC CAGAGAGAGG AAGCACAGCA GATGAAGCAG 
CGGAAAGATC AGCAGCAGCA GCAACAACAA CAACAACAAC CACCTCCACA GCCTCAGCCT 
CAGCAACATT CAACCAACAC CCAGCAAAAT CGCACACAAA ACCGGGCGGC CATCAATACG 
TCAGTGTGGG GTTCTGTCAA TAATGTGAGC TCAAACTGGA TGATGGACAG CAGTGTATGG 
GGCGACACGC AGAACTCTAA TATTGGCTTC TGGGATGAAG CAGTAAAGGA GGCCGCTCCA 
CCTCAAACCA CTCGCAAGAG CCACACTCCG AAAAACAAGG GCAACGCTAA CCTCAGTAAT 
AGCAGCAGCG GTAAAGCCAG TAAAAAGGTG GAAGAGGAGG AGAAGCTACT GAAACTGTTC 
CAGGGAGCCA ATAAGAACCA GGACGGCTTT ATGCAGTGGT GTGAACAGAC CCTGCACACT 
CTCAACACAG CCAATAACCT CGATGTTCCC ACTTTTGCAT CGTTCCTGAA GGAAGTGGAC 
TCTCCGTATG AGGTGCACGA TTATGTACGA GCTTATCTGG GTGACACTCC ACAGGCCAAG 
GACTTCGCCA AGCAGTTCCT GGAGCGCCGT GCCAAACAGA ACGACAACCA GCAGAAACCT 
CAGCAGGGCC AGCAACAAAA ACAGCAGGAG TCGGTGTGGG GAATAAGAGA AGTGCCACAG 
TCAGTCCTGC TTCAACAACA ACAACAACAA CAATTACAGC AGCAGCAACA GCAGCGGTTT 
GAAACGGTCA CTTCCGGCAA GAAGAAGAAG AAACAGAAGA TGGTACGAGC CGACCCAAGC 
CTTCTAGGTT TTTCTGTCAA TGCCTCCTCT GAGAGACTCA ATATGGGCGA GATCGAGACG 
GTTGAAGACT TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gigfy2 ( XM_017358654.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214143.1 Danio rerio GRB10 interacting GYF protein 2 (gigfy2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa56296
Clone ID Related Accession (Same CDS sequence)	XM_017358654.1 , XM_017358654.2
Accession Version	XM_017358654.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	GRB10-interacting GYF protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358654.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAAA CCCAGACACT CAACTTTGGG CCAGAATGGC TCCGTGCTCT GGCTGGTGGG 
GTGGGCAGCA GCATTGTGGC CTCCCCTCCG CTCTCGCCTG CATTGCCAAA GTATAAACTT 
GCAGACTATC GCTACGGGAG AGAAGAGATG CTAGCACTTT ATGTAAAGGA TAACATGATT 
CCGGTAGATC TTCATGATAA GGAATTCCTA CCTATTTTAC AAGAGGAGCC CCTGCCACCT 
TTGGCATTAG TCCCTTTTAC AGAGGAGGAA CAGAGAAATT TCTCCATGTC TGTAAACAGT 
GCAGCAGTTC TCCGTCTGAC AGGAAGGGGG GGAGGGACGG TGGCAGGGGC ACCTAGAGGC 
CGAAGTTCTT CACGAGGGCG AGGGAGAGGC AGAGGAGATG GTTTTTACCA AAGAAGTTTT 
GATGATGTGG AAGGAGGTTT CGGTCGTGGA GGGAGAGAGA TGCATCGCTC CCAGAGTTGG 
GAGGAGAGGG GAGACAGAAG GTTTGAAAAG CCAGGTCGAA AAGATCCAGA TGGAGCACCA 
GCCCATTTTC CACTCAATCA CATAAGGGCA AACTATGAGG ACCCTGCTGC AGTGAATGCC 
CGAAAGTTCA TTCATGCAGA GTGTGACAAC TGGCGCACCA CTCGAGATGA GCTGAATGGA 
GAGGAGGATG ATAACGGATG GAGGCTTGCA GGCGCTCGTC GGGAAAATGA GCGATGGTGT 
CCTCCAAGTC CAGATGGTCC ACGATCAGCA GGGTGGCGGG AACACCCAGA TCAACGTCGA 
CGCTTTGGTT TTGGGGATGA GGAACGCAGT GGTTACCGTA GGCCTCGGTC GGGCAGCGGG 
AGCGCAGAGG AGGAGAGGGA CAGTTTACCA GAATGGTGTC TGGAAGATGC AGAGGAGGAG 
ACAGGGACGT TTGACTCCTC AGGGGCCTTC CTCTCACTTA AGAAAGCTCC TAAGGAGCCA 
ATCCTGGAAG AGGCGGAGCT GGACTTCCGG CCATTGGAGG AGAATGACGA ATATGCAGAA 
AAGGATGACA GTGAAACTGA ACAAACCAAA GATACTGACA CAAATACTAG GCACGAAAGT 
GATCGAAATG AAGAAAACTG TAAATCAGAA GAGCCGTCGC CAGTTGCGGT ACCATTTTCA 
GCAGTAGTTA CGCCTCCCAA AGCCACCACC CCTGCACCCA TTCAGCCTGT ACACCTAGAA 
AAAGCTGAAG ATAAGGAGAG ACCGTCAGAG AGAACAACAT TACCAGAAAT CAGACATGAA 
CTGAGTAAAG CACCACTACA CACAGCCCTA TCTAACAGCA TTGTGGAAGC CATTTCCATA 
CCGCATGTCG CAAACAAACT CCCAGATCTT CCGGTCCCAG CGCCTTCAGT TCTGCCTGTC 
AAGTCAGTGC CCCCTCAATC CCAACAGGTC AAGCCTATAG AGATGCCAGT GTCAGTTCCT 
CCTGCTCTGC TACGCTCCAC AGGCAGTGTG GGTCCTATCA GTCGACCCTC TGCCCTGCCA 
TCGGACTTGG ATGAGGATGA GGGACTCAAG CACTTTGAGC AGGAAGCTGA GAAAATGGTG 
GCGTACCTCC AGGATGGTGG CGTAGATGAT GAACGTTTGG CCTCTAAAAT TGGACCCAAA 
CCATCAGCTC TTCCAATCAC AAATGAAGCT GCATTCAAAT GGTTTTACAA AGATCCGCAG 
GGGGAAATCC AAGGACCATT TAATAATCAG GAGATGTCTG AGTGGTTTCA GGCGGGCTAT 
TTCACCATGA CCTTGCAGGT AAAGCGAGGA TGCGACGAGA TGTTCCAGCC TCTGGGCGAG 
ATGATTAAGT TGTGGGGACG AGTGCCCTTT ACACCGGGCC CGACACTACC TCCCATACTG 
GGAGATGCAG ATCAAGAAAG GATGAAGAGA CAACAGGAGA TTAATGCTCT AAATATGTAT 
CAGCTACAGC AGATGCAATA TCAGTATTTA CTCAGGCAAC AATATGCTCT GCAGCAGAAA 
GTGTTAAACT CTGCTCCTCC TCCTCCTCCA CCACCACAGC AGCAGCTCAA TCTTCTTCTC 
CATCAGGCGC TGAAGATCAG AACTCCTGAG CCGCAGCAGA GCCTCTTACC TCCCGTCACA 
CGCTCCATGT CGGTTCCAGA CTCGGGTTCT GTTTGGGAAA TGCAGAACCC GTCCACTCAG 
GCATCCTGTT CTCCAAACAT GCAGCCCGCC GCTCCCAGCA CTTGGGAAGG GAGTAGCGTA 
TGGGATCTGC CACTAGACAA TATGCCTCAA GCGTCTTCCA TTGAACAGCT GCAGCTGGAG 
AAAGCCAAAG CTTTGAAGCT GGAGATGGAG AGACGGGAGG CAGAGCTTCG GGCCAAAAGG 
GAAGAAGAGG AGAGGAAGCG TTTGGAAGAG GCTCTGCGTG CGAGACAAGA GGAAGAGAGG 
AAGCGTTTAG AGGAAGAAGA GCTGGCACGA CGCAAGCAGG AGGAGGCTCT GAAGAGACAG 
CGGGAGCAGG AGGAGGCGCA GCGGAGGAAA AAGGAAGAGG AGGAACGGTT AGCTCAGGAG 
GAGGCTCTCC GGCGACTGGA GGAGCGGAGG AGAGAAGAAG AGGAGAGGAG ACAAAGGGAA 
GAATTCCTCC GCAAACAGCA GGAAGAGGAG CGGAGGAAGC AGGAAGAACT GGAAGCTCAG 
AGGCGGCGTG AGGAAGAGAA GAGGCTGGAG GAGGAGGCTG CGGCCGCTGC AGCAGCACTG 
CTCAGACAAC AGCAGGAGGA GCAGAAGAAG AGAGAGCAGG AGGCGCAGAG ACAGCAGGAG 
CTGCAGAGAC AGAGACAGCA GCAACAGGAG GCACTCCGAC GACTCCAGCA GCAGCAGCAA 
CAACAGCAGC TCGCACAAAT GAAGCTACCT TCATCCTCAA AGTGGGGTCA GCAGTCGACC 
ACAGCAAACA GTCTCTCACA GTCTCAAAAT GCTCTTTCGC TCGCTGAGAT CCAGAAACTG 
GAGGAGGAGA GAGAACGACA GACTCGAGAA GAGCAGAGAC GTCAGCAGCA GGAGCTCCAG 
CGGGTCCAGC AGCAGCAGCC TCAGACCAAG CTCCCTGGCT GGGGCAGTGT GGCTAAGCAG 
CCCATGGCCA CTAAATCCCT GTTGGAGATC CAGAGAGAGG AAGCACAGCA GATGAAGCAG 
CGGAAAGATC AGCAGCAGCA GCAACAACAA CAACAACAAC CACCTCCACA GCCTCAGCCT 
CAGCAACATT CAACCAACAC CCAGCAAAAT CGCACACAAA ACCGGGCGGC CATCAATACG 
TCAGTGTGGG GTTCTGTCAA TAATGTGAGC TCAAACTGGA TGATGGACAG CAGTGTATGG 
GGCGACACGC AGAACTCTAA TATTGGCTTC TGGGATGAAG CAGTAAAGGA GGCCGCTCCA 
CCTCAAACCA CTCGCAAGAG CCACACTCCG AAAAACAAGG GCAACGCTAA CCTCAGTAAT 
AGCAGCAGCG GTAAAGCCAG TAAAAAGGTG GAAGAGGAGG AGAAGCTACT GAAACTGTTC 
CAGGGAGCCA ATAAGAACCA GGACGGCTTT ATGCAGTGGT GTGAACAGAC CCTGCACACT 
CTCAACACAG CCAATAACCT CGATGTTCCC ACTTTTGCAT CGTTCCTGAA GGAAGTGGAC 
TCTCCGTATG AGGTGCACGA TTATGTACGA GCTTATCTGG GTGACACTCC ACAGGCCAAG 
GACTTCGCCA AGCAGTTCCT GGAGCGCCGT GCCAAACAGA ACGACAACCA GCAGAAACCT 
CAGCAGGGCC AGCAACAAAA ACAGCAGGAG TCGGTGTGGG GAATAAGAGA AGTGCCACAG 
TCAGTCCTGC TTCAACAACA ACAACAACAA CAATTACAGC AGCAGCAACA GCAGCGGTTT 
GAAACGGTCA CTTCCGGCAA GAAGAAGAAG AAACAGAAGA TGGTACGAGC CGACCCAAGC 
CTTCTAGGTT TTTCTGTCAA TGCCTCCTCT GAGAGACTCA ATATGGGCGA GATCGAGACG 
GTTGAAGACT TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214143.1
CDS	143..4117
Translation	MAETQTLNFGPEWLRALAGGVGSSIVASPPLSPALPKYKLADYRYGREEMLALYVKDNMIPVDLHDKEFLPILQEEPLPPLALVPFTEEEQRNFSMSVNSAAVLRLTGRGGGTVAGAPRGRSSSRGRGRGRGDGFYQRSFDDVEGGFGRGGREMHRSQSWEERGDRRFEKPGRKDPDGAPAHFPLNHIRANYEDPAAVNARKFIHAECDNWRTTRDELNGEEDDNGWRLAGARRENERWCPPSPDGPRSAGWREHPDQRRRFGFGDEERSGYRRPRSGSGSAEEERDSLPEWCLEDAEEETGTFDSSGAFLSLKKAPKEPILEEAELDFRPLEENDEYAEKDDSETEQTKDTDTNTRHESDRNEENCKSEEPSPVAVPFSAVVTPPKATTPAPIQPVHLEKAEDKERPSERTTLPEIRHELSKAPLHTALSNSIVEAISIPHVANKLPDLPVPAPSVLPVKSVPPQSQQVKPIEMPVSVPPALLRSTGSVGPISRPSALPSDLDEDEGLKHFEQEAEKMVAYLQDGGVDDERLASKIGPKPSALPITNEAAFKWFYKDPQGEIQGPFNNQEMSEWFQAGYFTMTLQVKRGCDEMFQPLGEMIKLWGRVPFTPGPTLPPILGDADQERMKRQQEINALNMYQLQQMQYQYLLRQQYALQQKVLNSAPPPPPPPQQQLNLLLHQALKIRTPEPQQSLLPPVTRSMSVPDSGSVWEMQNPSTQASCSPNMQPAAPSTWEGSSVWDLPLDNMPQASSIEQLQLEKAKALKLEMERREAELRAKREEEERKRLEEALRARQEEERKRLEEEELARRKQEEALKRQREQEEAQRRKKEEEERLAQEEALRRLEERRREEEERRQREEFLRKQQEEERRKQEELEAQRRREEEKRLEEEAAAAAAALLRQQQEEQKKREQEAQRQQELQRQRQQQQEALRRLQQQQQQQQLAQMKLPSSSKWGQQSTTANSLSQSQNALSLAEIQKLEEERERQTREEQRRQQQELQRVQQQQPQTKLPGWGSVAKQPMATKSLLEIQREEAQQMKQRKDQQQQQQQQQQPPPQPQPQQHSTNTQQNRTQNRAAINTSVWGSVNNVSSNWMMDSSVWGDTQNSNIGFWDEAVKEAAPPQTTRKSHTPKNKGNANLSNSSSGKASKKVEEEEKLLKLFQGANKNQDGFMQWCEQTLHTLNTANNLDVPTFASFLKEVDSPYEVHDYVRAYLGDTPQAKDFAKQFLERRAKQNDNQQKPQQGQQQKQQESVWGIREVPQSVLLQQQQQQQLQQQQQQRFETVTSGKKKKKQKMVRADPSLLGFSVNASSERLNMGEIETVEDF

Target ORF information:

RefSeq Version	XM_017358654.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio GRB10 interacting GYF protein 2 (gigfy2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358654.2

ORF Insert Sequence:

ATGGCGGAAA CCCAGACACT CAACTTTGGG CCAGAATGGC TCCGTGCTCT GGCTGGTGGG 
GTGGGCAGCA GCATTGTGGC CTCCCCTCCG CTCTCGCCTG CATTGCCAAA GTATAAACTT 
GCAGACTATC GCTACGGGAG AGAAGAGATG CTAGCACTTT ATGTAAAGGA TAACATGATT 
CCGGTAGATC TTCATGATAA GGAATTCCTA CCTATTTTAC AAGAGGAGCC CCTGCCACCT 
TTGGCATTAG TCCCTTTTAC AGAGGAGGAA CAGAGAAATT TCTCCATGTC TGTAAACAGT 
GCAGCAGTTC TCCGTCTGAC AGGAAGGGGG GGAGGGACGG TGGCAGGGGC ACCTAGAGGC 
CGAAGTTCTT CACGAGGGCG AGGGAGAGGC AGAGGAGATG GTTTTTACCA AAGAAGTTTT 
GATGATGTGG AAGGAGGTTT CGGTCGTGGA GGGAGAGAGA TGCATCGCTC CCAGAGTTGG 
GAGGAGAGGG GAGACAGAAG GTTTGAAAAG CCAGGTCGAA AAGATCCAGA TGGAGCACCA 
GCCCATTTTC CACTCAATCA CATAAGGGCA AACTATGAGG ACCCTGCTGC AGTGAATGCC 
CGAAAGTTCA TTCATGCAGA GTGTGACAAC TGGCGCACCA CTCGAGATGA GCTGAATGGA 
GAGGAGGATG ATAACGGATG GAGGCTTGCA GGCGCTCGTC GGGAAAATGA GCGATGGTGT 
CCTCCAAGTC CAGATGGTCC ACGATCAGCA GGGTGGCGGG AACACCCAGA TCAACGTCGA 
CGCTTTGGTT TTGGGGATGA GGAACGCAGT GGTTACCGTA GGCCTCGGTC GGGCAGCGGG 
AGCGCAGAGG AGGAGAGGGA CAGTTTACCA GAATGGTGTC TGGAAGATGC AGAGGAGGAG 
ACAGGGACGT TTGACTCCTC AGGGGCCTTC CTCTCACTTA AGAAAGCTCC TAAGGAGCCA 
ATCCTGGAAG AGGCGGAGCT GGACTTCCGG CCATTGGAGG AGAATGACGA ATATGCAGAA 
AAGGATGACA GTGAAACTGA ACAAACCAAA GATACTGACA CAAATACTAG GCACGAAAGT 
GATCGAAATG AAGAAAACTG TAAATCAGAA GAGCCGTCGC CAGTTGCGGT ACCATTTTCA 
GCAGTAGTTA CGCCTCCCAA AGCCACCACC CCTGCACCCA TTCAGCCTGT ACACCTAGAA 
AAAGCTGAAG ATAAGGAGAG ACCGTCAGAG AGAACAACAT TACCAGAAAT CAGACATGAA 
CTGAGTAAAG CACCACTACA CACAGCCCTA TCTAACAGCA TTGTGGAAGC CATTTCCATA 
CCGCATGTCG CAAACAAACT CCCAGATCTT CCGGTCCCAG CGCCTTCAGT TCTGCCTGTC 
AAGTCAGTGC CCCCTCAATC CCAACAGGTC AAGCCTATAG AGATGCCAGT GTCAGTTCCT 
CCTGCTCTGC TACGCTCCAC AGGCAGTGTG GGTCCTATCA GTCGACCCTC TGCCCTGCCA 
TCGGACTTGG ATGAGGATGA GGGACTCAAG CACTTTGAGC AGGAAGCTGA GAAAATGGTG 
GCGTACCTCC AGGATGGTGG CGTAGATGAT GAACGTTTGG CCTCTAAAAT TGGACCCAAA 
CCATCAGCTC TTCCAATCAC AAATGAAGCT GCATTCAAAT GGTTTTACAA AGATCCGCAG 
GGGGAAATCC AAGGACCATT TAATAATCAG GAGATGTCTG AGTGGTTTCA GGCGGGCTAT 
TTCACCATGA CCTTGCAGGT AAAGCGAGGA TGCGACGAGA TGTTCCAGCC TCTGGGCGAG 
ATGATTAAGT TGTGGGGACG AGTGCCCTTT ACACCGGGCC CGACACTACC TCCCATACTG 
GGAGATGCAG ATCAAGAAAG GATGAAGAGA CAACAGGAGA TTAATGCTCT AAATATGTAT 
CAGCTACAGC AGATGCAATA TCAGTATTTA CTCAGGCAAC AATATGCTCT GCAGCAGAAA 
GTGTTAAACT CTGCTCCTCC TCCTCCTCCA CCACCACAGC AGCAGCTCAA TCTTCTTCTC 
CATCAGGCGC TGAAGATCAG AACTCCTGAG CCGCAGCAGA GCCTCTTACC TCCCGTCACA 
CGCTCCATGT CGGTTCCAGA CTCGGGTTCT GTTTGGGAAA TGCAGAACCC GTCCACTCAG 
GCATCCTGTT CTCCAAACAT GCAGCCCGCC GCTCCCAGCA CTTGGGAAGG GAGTAGCGTA 
TGGGATCTGC CACTAGACAA TATGCCTCAA GCGTCTTCCA TTGAACAGCT GCAGCTGGAG 
AAAGCCAAAG CTTTGAAGCT GGAGATGGAG AGACGGGAGG CAGAGCTTCG GGCCAAAAGG 
GAAGAAGAGG AGAGGAAGCG TTTGGAAGAG GCTCTGCGTG CGAGACAAGA GGAAGAGAGG 
AAGCGTTTAG AGGAAGAAGA GCTGGCACGA CGCAAGCAGG AGGAGGCTCT GAAGAGACAG 
CGGGAGCAGG AGGAGGCGCA GCGGAGGAAA AAGGAAGAGG AGGAACGGTT AGCTCAGGAG 
GAGGCTCTCC GGCGACTGGA GGAGCGGAGG AGAGAAGAAG AGGAGAGGAG ACAAAGGGAA 
GAATTCCTCC GCAAACAGCA GGAAGAGGAG CGGAGGAAGC AGGAAGAACT GGAAGCTCAG 
AGGCGGCGTG AGGAAGAGAA GAGGCTGGAG GAGGAGGCTG CGGCCGCTGC AGCAGCACTG 
CTCAGACAAC AGCAGGAGGA GCAGAAGAAG AGAGAGCAGG AGGCGCAGAG ACAGCAGGAG 
CTGCAGAGAC AGAGACAGCA GCAACAGGAG GCACTCCGAC GACTCCAGCA GCAGCAGCAA 
CAACAGCAGC TCGCACAAAT GAAGCTACCT TCATCCTCAA AGTGGGGTCA GCAGTCGACC 
ACAGCAAACA GTCTCTCACA GTCTCAAAAT GCTCTTTCGC TCGCTGAGAT CCAGAAACTG 
GAGGAGGAGA GAGAACGACA GACTCGAGAA GAGCAGAGAC GTCAGCAGCA GGAGCTCCAG 
CGGGTCCAGC AGCAGCAGCC TCAGACCAAG CTCCCTGGCT GGGGCAGTGT GGCTAAGCAG 
CCCATGGCCA CTAAATCCCT GTTGGAGATC CAGAGAGAGG AAGCACAGCA GATGAAGCAG 
CGGAAAGATC AGCAGCAGCA GCAACAACAA CAACAACAAC CACCTCCACA GCCTCAGCCT 
CAGCAACATT CAACCAACAC CCAGCAAAAT CGCACACAAA ACCGGGCGGC CATCAATACG 
TCAGTGTGGG GTTCTGTCAA TAATGTGAGC TCAAACTGGA TGATGGACAG CAGTGTATGG 
GGCGACACGC AGAACTCTAA TATTGGCTTC TGGGATGAAG CAGTAAAGGA GGCCGCTCCA 
CCTCAAACCA CTCGCAAGAG CCACACTCCG AAAAACAAGG GCAACGCTAA CCTCAGTAAT 
AGCAGCAGCG GTAAAGCCAG TAAAAAGGTG GAAGAGGAGG AGAAGCTACT GAAACTGTTC 
CAGGGAGCCA ATAAGAACCA GGACGGCTTT ATGCAGTGGT GTGAACAGAC CCTGCACACT 
CTCAACACAG CCAATAACCT CGATGTTCCC ACTTTTGCAT CGTTCCTGAA GGAAGTGGAC 
TCTCCGTATG AGGTGCACGA TTATGTACGA GCTTATCTGG GTGACACTCC ACAGGCCAAG 
GACTTCGCCA AGCAGTTCCT GGAGCGCCGT GCCAAACAGA ACGACAACCA GCAGAAACCT 
CAGCAGGGCC AGCAACAAAA ACAGCAGGAG TCGGTGTGGG GAATAAGAGA AGTGCCACAG 
TCAGTCCTGC TTCAACAACA ACAACAACAA CAATTACAGC AGCAGCAACA GCAGCGGTTT 
GAAACGGTCA CTTCCGGCAA GAAGAAGAAG AAACAGAAGA TGGTACGAGC CGACCCAAGC 
CTTCTAGGTT TTTCTGTCAA TGCCTCCTCT GAGAGACTCA ATATGGGCGA GATCGAGACG 
GTTGAAGACT TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gigfy2 ( XM_017358657.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214146.1 Danio rerio GRB10 interacting GYF protein 2 (gigfy2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa56297
Clone ID Related Accession (Same CDS sequence)	XM_017358657.1
Accession Version	XM_017358657.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	PERQ amino acid-rich with GYF domain-containing protein 2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAAA CCCAGACACT CAACTTTGGG CCAGAATGGC TCCGTGCTCT GGCTGGTGGG 
GTGGGCAGCA GCATTGTGGC CTCCCCTCCG CTCTCGCCTG CATTGCCAAA GTATAAACTT 
GCAGACTATC GCTACGGGAG AGAAGAGATG CTAGCACTTT ATGTAAAGGA TAACATGATT 
CCGGTAGATC TTCATGATAA GGAATTCCTA CCTATTTTAC AAGAGGAGCC CCTGCCACCT 
TTGGCATTAG TCCCTTTTAC AGAGGAGGAA CAGAGAAATT TCTCCATGTC TGTAAACAGT 
GCAGCAGTTC TCCGTCTGAC AGGAAGGGGG GGAGGGACGG TGGCAGGGGC ACCTAGAGGC 
CGAAGTTCTT CACGAGGGCG AGGGAGAGGC AGAGGAGATG GTTTTTACCA AAGAAGTTTT 
GATGATGTGG AAGGAGGTTT CGGTCGTGGA GGGAGAGAGA TGCATCGCTC CCAGAGTTGG 
GAGGAGAGGG GAGACAGAAG GTTTGAAAAG CCAGGTCGAA AAGATCCAGA TGGAGCACCA 
GCCCATTTTC CACTCAATCA CATAAGGGCA AACTATGAGG ACCCTGCTGC AGTGAATGCC 
CGAAAGTTCA TTCATGCAGA GTGTGACAAC TGGCGCACCA CTCGAGATGA GCTGAATGGA 
GAGGAGGATG ATAACGGATG GAGGCTTGCA GGCGCTCGTC GGGAAAATGA GCGATGGTGT 
CCTCCAAGTC CAGATGGTCC ACGATCAGCA GGGTGGCGGG AACACCCAGA TCAACGTCGA 
CGCTTTGGTT TTGGGGATGA GGAACGCAGT GGTTACCGTA GGCCTCGGTC GGGCAGCGGG 
AGCGCAGAGG AGGAGAGGGA CAGTTTACCA GAATGGTGTC TGGAAGATGC AGAGGAGGAG 
ACAGGGACGT TTGACTCCTC AGGGGCCTTC CTCTCACTTA AGAAAGCTCC TAAGGAGCCA 
ATCCTGGAAG AGGCGGAGCT GGACTTCCGG CCATTGGAGG AGAATGACGA ATATGCAGAA 
AAGGATGACA GTGAAACTGA ACAAACCAAA GATACTGACA CAAATACTAG GCACGAAAGT 
GATCGAAATG AAGAAAACTG TAAATCAGAA GAGCCGTCGC CAGTTGCGGT ACCATTTTCA 
GCAGTAGTTA CGCCTCCCAA AGCCACCACC CCTGCACCCA TTCAGCCTGT ACACCTAGAA 
AAAGCTGAAG ATAAGGAGAG ACCGTCAGAG AGAACAACAT TACCAGAAAT CAGACATGAA 
CTGAGTAAAG CACCACTACA CACAGCCCTA TCTAACAGCA TTGTGGAAGC CATTTCCATA 
CCGCATGTCG CAAACAAACT CCCAGATCTT CCGGTCCCAG CGCCTTCAGT TCTGCCTGTC 
AAGTCAGTGC CCCCTCAATC CCAACAGGTC AAGCCTATAG AGATGCCAGT GTCAGTTCCT 
CCTGCTCTGC TACGCTCCAC AGGCAGTGTG GGTCCTATCA GTCGACCCTC TGCCCTGCCA 
TCGGACTTGG ATGAGGATGA GGGACTCAAG CACTTTGAGC AGGAAGCTGA GAAAATGGTG 
GCGTACCTCC AGGATGGTGG CGTAGATGAT GAACGTTTGG CCTCTAAAAT TGGACCCAAA 
CCATCAGCTC TTCCAATCAC AAATGAAGCT GCATTCAAAT GGTTTTACAA AGATCCGCAG 
GGGGAAATCC AAGGACCATT TAATAATCAG GAGATGTCTG AGTGGTTTCA GGCGGGCTAT 
TTCACCATGA CCTTGCAGGT AAAGCGAGGA TGCGACGAGA TGTTCCAGCC TCTGGGCGAG 
ATGATTAAGT TGTGGGGACG AGTGCCCTTT ACACCGGGCC CGACACTACC TCCCATACTG 
GGAGATGCAG ATCAAGAAAG GATGAAGAGA CAACAGGAGA TTAATGCTCT AAATATGTAT 
CAGCTACAGC AGATGCAATA TCAGTATTTA CTCAGGCAAC AATATGCTCT GCAGCAGAAA 
GTGTTAAACT CTGCTCCTCC TCCTCCTCCA CCACCACAGC AGCAGCTCAA TCTTCTTCTC 
CATCAGGCGC TGAAGATCAG AACTCCTGAG CCGCAGCAGA GCCTCTTACC TCCCGTCACA 
CGCTCCATGT CGGTTCCAGA CTCGGGTTCT GTTTGGGAAA TGCAGAACCC GTCCACTCAG 
GCATCCTGTT CTCCAAACAT GCAGCCCGCC GCTCCCAGCA CTTGGGAAGG GAGTAGCGTA 
TGGGATCTGC CACTAGACAA TATGCCTCAA GCGTCTTCCA TTGAACAGCT GCAGCTGGAG 
AAAGCCAAAG CTTTGAAGCT GGAGATGGAG AGACGGGAGG CAGAGCTTCG GGCCAAAAGG 
GAAGAAGAGG AGAGGAAGCG TTTGGAAGAG GCTCTGCGTG CGAGACAAGA GGAAGAGAGG 
AAGCGTTTAG AGGAAGAAGA GCTGGCACGA CGCAAGCAGG AGGAGGCTCT GAAGAGACAG 
CGGGAGCAGG AGGAGGCGCA GCGGAGGAAA AAGGAAGAGG AGGAACGGTT AGCTCAGGAG 
GAGGCTCTCC GGCGACTGGA GGAGCGGAGG AGAGAAGAAG AGGAGAGGAG ACAAAGGGAA 
GAATTCCTCC GCAAACAGGA AGAGGAGCGG AGGAAGCAGG AAGAACTGGA AGCTCAGAGG 
CGGCGTGAGG AAGAGAAGAG GCTGGAGGAG GAGGCTGCGG CCGCTGCAGC AGCACTGCTC 
AGACAACAGC AGGAGGAGCA GAAGAAGAGA GAGCAGGAGG CGCAGAGACA GCAGGAGCTG 
CAGAGACAGA GACAGCAGCA ACAGGAGGCA CTCCGACGAC TCCAGCAGCA GCAGCAACAA 
CAGCAGCTCG CACAAATGAA GCTACCTTCA TCCTCAAAGT GGGGTCAGCA GTCGACCACA 
GCAAACAGTC TCTCACAGTC TCAAAATGCT CTTTCGCTCG CTGAGATCCA GAAACTGGAG 
GAGGAGAGAG AACGACAGAC TCGAGAAGAG CAGAGACGTC AGCAGCAGGA GCTCCAGCGG 
GTCCAGCAGC AGCAGCCTCA GACCAAGCTC CCTGGCTGGG GCAGTGTGGC TAAGCAGCCC 
ATGGCCACTA AATCCCTGTT GGAGATCCAG AGAGAGGAAG CACAGCAGAT GAAGCAGCGG 
AAAGATCAGC AGCAGCAGCA ACAACAACAA CAACAACCAC CTCCACAGCC TCAGCCTCAG 
CAACATTCAA CCAACACCCA GCAAAATCGC ACACAAAACC GGGCGGCCAT CAATACGTCA 
GTGTGGGGTT CTGTCAATAA TGTGAGCTCA AACTGGATGA TGGACAGCAG TGTATGGGGC 
GACACGCAGA ACTCTAATAT TGGCTTCTGG GATGAAGCAG TAAAGGAGGC CGCTCCACCT 
CAAACCACTC GCAAGAGCCA CACTCCGAAA AACAAGGGCA ACGCTAACCT CAGTAATAGC 
AGCAGCGGTA AAGCCAGTAA AAAGGTGGAA GAGGAGGAGA AGCTACTGAA ACTGTTCCAG 
GGAGCCAATA AGAACCAGGA CGGCTTTATG CAGTGGTGTG AACAGACCCT GCACACTCTC 
AACACAGCCA ATAACCTCGA TGTTCCCACT TTTGCATCGT TCCTGAAGGA AGTGGACTCT 
CCGTATGAGG TGCACGATTA TGTACGAGCT TATCTGGGTG ACACTCCACA GGCCAAGGAC 
TTCGCCAAGC AGTTCCTGGA GCGCCGTGCC AAACAGAACG ACAACCAGCA GAAACCTCAG 
CAGGGCCAGC AACAAAAACA GCAGGAGTCG GTGTGGGGAA TAAGAGAAGT GCCACAGTCA 
GTCCTGCTTC AACAACAACA ACAACAACAA TTACAGCAGC AGCAACAGCA GCGGTTTGAA 
ACGGTCACTT CCGGCAAGAA GAAGAAGAAA CAGAAGATGG TACGAGCCGA CCCAAGCCTT 
CTAGGTTTTT CTGTCAATGC CTCCTCTGAG AGACTCAATA TGGGCGAGAT CGAGACGGTT 
GAAGACTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214146.1
CDS	102..4073
Translation	MAETQTLNFGPEWLRALAGGVGSSIVASPPLSPALPKYKLADYRYGREEMLALYVKDNMIPVDLHDKEFLPILQEEPLPPLALVPFTEEEQRNFSMSVNSAAVLRLTGRGGGTVAGAPRGRSSSRGRGRGRGDGFYQRSFDDVEGGFGRGGREMHRSQSWEERGDRRFEKPGRKDPDGAPAHFPLNHIRANYEDPAAVNARKFIHAECDNWRTTRDELNGEEDDNGWRLAGARRENERWCPPSPDGPRSAGWREHPDQRRRFGFGDEERSGYRRPRSGSGSAEEERDSLPEWCLEDAEEETGTFDSSGAFLSLKKAPKEPILEEAELDFRPLEENDEYAEKDDSETEQTKDTDTNTRHESDRNEENCKSEEPSPVAVPFSAVVTPPKATTPAPIQPVHLEKAEDKERPSERTTLPEIRHELSKAPLHTALSNSIVEAISIPHVANKLPDLPVPAPSVLPVKSVPPQSQQVKPIEMPVSVPPALLRSTGSVGPISRPSALPSDLDEDEGLKHFEQEAEKMVAYLQDGGVDDERLASKIGPKPSALPITNEAAFKWFYKDPQGEIQGPFNNQEMSEWFQAGYFTMTLQVKRGCDEMFQPLGEMIKLWGRVPFTPGPTLPPILGDADQERMKRQQEINALNMYQLQQMQYQYLLRQQYALQQKVLNSAPPPPPPPQQQLNLLLHQALKIRTPEPQQSLLPPVTRSMSVPDSGSVWEMQNPSTQASCSPNMQPAAPSTWEGSSVWDLPLDNMPQASSIEQLQLEKAKALKLEMERREAELRAKREEEERKRLEEALRARQEEERKRLEEEELARRKQEEALKRQREQEEAQRRKKEEEERLAQEEALRRLEERRREEEERRQREEFLRKQEEERRKQEELEAQRRREEEKRLEEEAAAAAAALLRQQQEEQKKREQEAQRQQELQRQRQQQQEALRRLQQQQQQQQLAQMKLPSSSKWGQQSTTANSLSQSQNALSLAEIQKLEEERERQTREEQRRQQQELQRVQQQQPQTKLPGWGSVAKQPMATKSLLEIQREEAQQMKQRKDQQQQQQQQQQPPPQPQPQQHSTNTQQNRTQNRAAINTSVWGSVNNVSSNWMMDSSVWGDTQNSNIGFWDEAVKEAAPPQTTRKSHTPKNKGNANLSNSSSGKASKKVEEEEKLLKLFQGANKNQDGFMQWCEQTLHTLNTANNLDVPTFASFLKEVDSPYEVHDYVRAYLGDTPQAKDFAKQFLERRAKQNDNQQKPQQGQQQKQQESVWGIREVPQSVLLQQQQQQQLQQQQQQRFETVTSGKKKKKQKMVRADPSLLGFSVNASSERLNMGEIETVEDF

Target ORF information:

RefSeq Version	XM_017358657.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio GRB10 interacting GYF protein 2 (gigfy2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358657.1

ORF Insert Sequence:

ATGGCGGAAA CCCAGACACT CAACTTTGGG CCAGAATGGC TCCGTGCTCT GGCTGGTGGG 
GTGGGCAGCA GCATTGTGGC CTCCCCTCCG CTCTCGCCTG CATTGCCAAA GTATAAACTT 
GCAGACTATC GCTACGGGAG AGAAGAGATG CTAGCACTTT ATGTAAAGGA TAACATGATT 
CCGGTAGATC TTCATGATAA GGAATTCCTA CCTATTTTAC AAGAGGAGCC CCTGCCACCT 
TTGGCATTAG TCCCTTTTAC AGAGGAGGAA CAGAGAAATT TCTCCATGTC TGTAAACAGT 
GCAGCAGTTC TCCGTCTGAC AGGAAGGGGG GGAGGGACGG TGGCAGGGGC ACCTAGAGGC 
CGAAGTTCTT CACGAGGGCG AGGGAGAGGC AGAGGAGATG GTTTTTACCA AAGAAGTTTT 
GATGATGTGG AAGGAGGTTT CGGTCGTGGA GGGAGAGAGA TGCATCGCTC CCAGAGTTGG 
GAGGAGAGGG GAGACAGAAG GTTTGAAAAG CCAGGTCGAA AAGATCCAGA TGGAGCACCA 
GCCCATTTTC CACTCAATCA CATAAGGGCA AACTATGAGG ACCCTGCTGC AGTGAATGCC 
CGAAAGTTCA TTCATGCAGA GTGTGACAAC TGGCGCACCA CTCGAGATGA GCTGAATGGA 
GAGGAGGATG ATAACGGATG GAGGCTTGCA GGCGCTCGTC GGGAAAATGA GCGATGGTGT 
CCTCCAAGTC CAGATGGTCC ACGATCAGCA GGGTGGCGGG AACACCCAGA TCAACGTCGA 
CGCTTTGGTT TTGGGGATGA GGAACGCAGT GGTTACCGTA GGCCTCGGTC GGGCAGCGGG 
AGCGCAGAGG AGGAGAGGGA CAGTTTACCA GAATGGTGTC TGGAAGATGC AGAGGAGGAG 
ACAGGGACGT TTGACTCCTC AGGGGCCTTC CTCTCACTTA AGAAAGCTCC TAAGGAGCCA 
ATCCTGGAAG AGGCGGAGCT GGACTTCCGG CCATTGGAGG AGAATGACGA ATATGCAGAA 
AAGGATGACA GTGAAACTGA ACAAACCAAA GATACTGACA CAAATACTAG GCACGAAAGT 
GATCGAAATG AAGAAAACTG TAAATCAGAA GAGCCGTCGC CAGTTGCGGT ACCATTTTCA 
GCAGTAGTTA CGCCTCCCAA AGCCACCACC CCTGCACCCA TTCAGCCTGT ACACCTAGAA 
AAAGCTGAAG ATAAGGAGAG ACCGTCAGAG AGAACAACAT TACCAGAAAT CAGACATGAA 
CTGAGTAAAG CACCACTACA CACAGCCCTA TCTAACAGCA TTGTGGAAGC CATTTCCATA 
CCGCATGTCG CAAACAAACT CCCAGATCTT CCGGTCCCAG CGCCTTCAGT TCTGCCTGTC 
AAGTCAGTGC CCCCTCAATC CCAACAGGTC AAGCCTATAG AGATGCCAGT GTCAGTTCCT 
CCTGCTCTGC TACGCTCCAC AGGCAGTGTG GGTCCTATCA GTCGACCCTC TGCCCTGCCA 
TCGGACTTGG ATGAGGATGA GGGACTCAAG CACTTTGAGC AGGAAGCTGA GAAAATGGTG 
GCGTACCTCC AGGATGGTGG CGTAGATGAT GAACGTTTGG CCTCTAAAAT TGGACCCAAA 
CCATCAGCTC TTCCAATCAC AAATGAAGCT GCATTCAAAT GGTTTTACAA AGATCCGCAG 
GGGGAAATCC AAGGACCATT TAATAATCAG GAGATGTCTG AGTGGTTTCA GGCGGGCTAT 
TTCACCATGA CCTTGCAGGT AAAGCGAGGA TGCGACGAGA TGTTCCAGCC TCTGGGCGAG 
ATGATTAAGT TGTGGGGACG AGTGCCCTTT ACACCGGGCC CGACACTACC TCCCATACTG 
GGAGATGCAG ATCAAGAAAG GATGAAGAGA CAACAGGAGA TTAATGCTCT AAATATGTAT 
CAGCTACAGC AGATGCAATA TCAGTATTTA CTCAGGCAAC AATATGCTCT GCAGCAGAAA 
GTGTTAAACT CTGCTCCTCC TCCTCCTCCA CCACCACAGC AGCAGCTCAA TCTTCTTCTC 
CATCAGGCGC TGAAGATCAG AACTCCTGAG CCGCAGCAGA GCCTCTTACC TCCCGTCACA 
CGCTCCATGT CGGTTCCAGA CTCGGGTTCT GTTTGGGAAA TGCAGAACCC GTCCACTCAG 
GCATCCTGTT CTCCAAACAT GCAGCCCGCC GCTCCCAGCA CTTGGGAAGG GAGTAGCGTA 
TGGGATCTGC CACTAGACAA TATGCCTCAA GCGTCTTCCA TTGAACAGCT GCAGCTGGAG 
AAAGCCAAAG CTTTGAAGCT GGAGATGGAG AGACGGGAGG CAGAGCTTCG GGCCAAAAGG 
GAAGAAGAGG AGAGGAAGCG TTTGGAAGAG GCTCTGCGTG CGAGACAAGA GGAAGAGAGG 
AAGCGTTTAG AGGAAGAAGA GCTGGCACGA CGCAAGCAGG AGGAGGCTCT GAAGAGACAG 
CGGGAGCAGG AGGAGGCGCA GCGGAGGAAA AAGGAAGAGG AGGAACGGTT AGCTCAGGAG 
GAGGCTCTCC GGCGACTGGA GGAGCGGAGG AGAGAAGAAG AGGAGAGGAG ACAAAGGGAA 
GAATTCCTCC GCAAACAGGA AGAGGAGCGG AGGAAGCAGG AAGAACTGGA AGCTCAGAGG 
CGGCGTGAGG AAGAGAAGAG GCTGGAGGAG GAGGCTGCGG CCGCTGCAGC AGCACTGCTC 
AGACAACAGC AGGAGGAGCA GAAGAAGAGA GAGCAGGAGG CGCAGAGACA GCAGGAGCTG 
CAGAGACAGA GACAGCAGCA ACAGGAGGCA CTCCGACGAC TCCAGCAGCA GCAGCAACAA 
CAGCAGCTCG CACAAATGAA GCTACCTTCA TCCTCAAAGT GGGGTCAGCA GTCGACCACA 
GCAAACAGTC TCTCACAGTC TCAAAATGCT CTTTCGCTCG CTGAGATCCA GAAACTGGAG 
GAGGAGAGAG AACGACAGAC TCGAGAAGAG CAGAGACGTC AGCAGCAGGA GCTCCAGCGG 
GTCCAGCAGC AGCAGCCTCA GACCAAGCTC CCTGGCTGGG GCAGTGTGGC TAAGCAGCCC 
ATGGCCACTA AATCCCTGTT GGAGATCCAG AGAGAGGAAG CACAGCAGAT GAAGCAGCGG 
AAAGATCAGC AGCAGCAGCA ACAACAACAA CAACAACCAC CTCCACAGCC TCAGCCTCAG 
CAACATTCAA CCAACACCCA GCAAAATCGC ACACAAAACC GGGCGGCCAT CAATACGTCA 
GTGTGGGGTT CTGTCAATAA TGTGAGCTCA AACTGGATGA TGGACAGCAG TGTATGGGGC 
GACACGCAGA ACTCTAATAT TGGCTTCTGG GATGAAGCAG TAAAGGAGGC CGCTCCACCT 
CAAACCACTC GCAAGAGCCA CACTCCGAAA AACAAGGGCA ACGCTAACCT CAGTAATAGC 
AGCAGCGGTA AAGCCAGTAA AAAGGTGGAA GAGGAGGAGA AGCTACTGAA ACTGTTCCAG 
GGAGCCAATA AGAACCAGGA CGGCTTTATG CAGTGGTGTG AACAGACCCT GCACACTCTC 
AACACAGCCA ATAACCTCGA TGTTCCCACT TTTGCATCGT TCCTGAAGGA AGTGGACTCT 
CCGTATGAGG TGCACGATTA TGTACGAGCT TATCTGGGTG ACACTCCACA GGCCAAGGAC 
TTCGCCAAGC AGTTCCTGGA GCGCCGTGCC AAACAGAACG ACAACCAGCA GAAACCTCAG 
CAGGGCCAGC AACAAAAACA GCAGGAGTCG GTGTGGGGAA TAAGAGAAGT GCCACAGTCA 
GTCCTGCTTC AACAACAACA ACAACAACAA TTACAGCAGC AGCAACAGCA GCGGTTTGAA 
ACGGTCACTT CCGGCAAGAA GAAGAAGAAA CAGAAGATGG TACGAGCCGA CCCAAGCCTT 
CTAGGTTTTT CTGTCAATGC CTCCTCTGAG AGACTCAATA TGGGCGAGAT CGAGACGGTT 
GAAGACTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine induced Parkinson's disease in zebrafish.
Proteomics16(9)1407-20(2016 05)
Sarath Babu N,Murthy ChL,Kakara S,Sharma R,Brahmendra Swamy CV,Idris MM

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Mutational screening and zebrafish functional analysis of GIGYF2 as a Parkinson-disease gene.
Neurobiology of aging32(11)1994-2005(2011 Nov)
Guella I,Pistocchi A,Asselta R,Rimoldi V,Ghilardi A,Sironi F,Trotta L,Primignani P,Zini M,Zecchinelli A,Coviello D,Pezzoli G,Del Giacco L,Duga S,Goldwurm S

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gigfy2 cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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