pum2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pum2
Entrez Gene ID	567494
Full Name	pumilio RNA-binding family member 2
Synonyms	Pumilio2,si:ch1073-351m12.1
General protein information	Preferred Names pumilio RNA-binding family member 2 Names pumilio RNA-binding family member 2 pumilio homolog 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001102570.1	NP_001096040.1	pumilio RNA-binding family member 2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Extensive alternative polyadenylation during zebrafish development.
Ulitsky I, Shkumatava A, Jan CH, Subtelny AO, Koppstein D, Bell GW, Sive H, Bartel DP
Genome research22(10)2054-66(2012 Oct)

Identification and characterization of the pumilio-2 expressed in zebrafish embryos and adult tissues.
Wang HN, Xu Y, Tao LJ, Zhou J, Qiu MX, Teng YH, Deng FJ
Molecular biology reports39(3)2811-9(2012 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

pumilio-2 plays an important role in the development of the zebrafish germ cells and nervous system.
Title: Identification and characterization of the pumilio-2 expressed in zebrafish embryos and adult tissues.

The following pum2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pum2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02648	NM_001102570.1 Latest version!	Danio rerio pumilio RNA-binding family member 2 (pum2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pum2 ( NM_001102570.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001096040.1 Danio rerio pumilio RNA-binding family member 2 (pum2), mRNA.

Price & Availability↑

CloneID	ODa02648
Clone ID Related Accession (Same CDS sequence)	NM_001102570.1
Accession Version	NM_001102570.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	pumilio RNA-binding family member 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EU003998.1. On Aug 29, 2007 this sequence version replaced XM_690791.2. ##Evidence-Data-START## Transcript exon combination :: EU003998.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA4476738, SAMEA4476771 [ECO:0000350] ##Evidence-Data-END##

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTAC CCTGGCAGGA GACACAATGT 
GGAACGCTGC ACACCAATGG TGCACCAGAA ACTGGTGGGG TCAGAGTCCA TGCAGGGTGT 
CCTCTAGCCA CGGTTGGAGG TGCCAGGCAG GTACCTGGAG GAGTTCACCC TTTGCAGGGA 
ATGGCCAATC CCAACCCCTG CACCCAGCCT CTTCTGAGTG GTCGTTCACA AGATGATGCC 
ACTGTGGGCT ATTTCTTTCA AAGGCAGCCT GGAGAGCAAA TGGGAGGCTG CACTGGCAAC 
AAACACCGCT GGCCCACTGG TGATGGAAAC CATGTTGACC AGTTGCGCTC TGTGGATGAG 
ATGAACTATA ACTTTCAAGC ATTGGCTCTG GAGTCTCGCG GAATGGGTGA ACTACTTCCT 
GCAAAAAAAT TGTGGGAATC AGATGAATTG GCAAAAGATG GACGTAAAGC AATGCTTGTG 
GGTGAAGAAT GGAGAGAGAA TGCCTGGAGT TCTTCTCATC ATGCTCTCTC TCAACCCATT 
ATGGTGCAGA AGCGACCAGG CCAAGGCTAC AATGGGACTG GAGAGACAAG CTCAGTGCTG 
TCCCCACGAT CAGAGGGGGG TGGTCTTGGT GTGAGTATGG TGGAGTATGT CCTCAGCTCC 
TCCCCTGGAG ACAAGATGGA TGGTCGCTAC AGGAATGGAG GATATGGTGA AGCAGATGCA 
GATCCAGATG GAAGAGAAAA GGGAGAATCA AAAGAGAAGT CTTCACCTTT CGAAGAAAAC 
AAAAGTCCTG AGATGAAAGT TGGAGATGAA GGGGATACCT CAAAAGCAAA TGCCAGGGGA 
TTACTCAATG GAATGGACCG AGATTGCAAA GACTTCAACC CCACTCCAGG TAGTCGCCAG 
GCATCCCCCA CAGAAGCAGT AGAACGACTG GGACCCAGTC AGGCTGGGCT GGAAATAATG 
GTGCAACACC ACCAGCACAC TCTGCAGGCA CCCAACCCTG TTACAAACAA ACCTCCTCCT 
GAAGACTTCC AGAGTCAGGA AGCCCAGAAC ATGGGTGGCA TGGAGCAGCA GGCGGGGCTG 
GAGGCTCTTC AGTTTGACTA TGGTGGAAAT CAGATCCAGT TGGACTCATC AGGTGCTGCA 
GTCGGCCTAT TTAACTACGA CTCTCAACAG CAGTTGTTCC AGAGATCCAG TCATCTCACA 
GTTCAGCAGC TGAATGCAGC ACAGCAACAA CAGTACGCCC TTGCTGCAGC CCAACAGCAA 
CACCTTGGCC TTGCACCTGC ATTTGTGCCA AACCCTTACA TCATCAATGC TGCCCCTCCT 
GGAGCAGACC CCTACACTGC TGCAGGATTA GCAGCAGCTG CAACACTTGC AGGTCCAGCT 
GTAGTTCCGC CCCAGTACTA TGGGGTCCCT TGGGGTGTGT ATCCAGCCAA TCTCTTCCAA 
CAACAAGCTA CAGCTGCTGC CAGTCATTCT GCTAACCAGC AGGCATCCAG TCAGGGTCCA 
GGGCAACAGG TGATTCGAGC AGGGTCCAAT CAACGACCTT TAACTCCAGG ACAGAGCCAA 
CAGAGTCAGC AGGAGTCGCT TGCTGCAGCT GCCACAAACC CAACACTGGC CTTTCCAGGC 
ATGTCAGGCT ACCAGGTCCT AGCGCCGGCT GCTTATTATG ATCAGACTGG TGCATTGGTC 
ATGGGTCCTG GAACTCGCAC TGGCCTGGGT GGACCTGTTC GACTGGTCCA AACCCCTCTT 
CTCATCAACC CAGCAGCCGC ACAAGCTGCT GCAGCTGCAT CTGTATCAGG CTCTGGTAAT 
AGTATGTCTG GTCCTGCTAA TGGTCTATAC CGGTCCATGG CATGCCAACA ACCCCAGCAG 
CCTCAACCTC AGCAGCAGCT TCCTCCACCT TCCAGCGGTC TGCCTTCAAA CTCCTTCTAT 
GGCTCTGGTT CTGTAGCACC CAGTTCCCAG AGCAGCTCAC TTTTCTCCCA TACAAGTGCA 
GCTCCTCCAC CTAGCACCTC TCTTGGTTTC AGCAGCACTA GTGGATCTTT AGGTGTTGGA 
TTAGGCTCTG CTCTTGGTGC ATTTGGCTCC TCAGTGTCCA GTTCCACTAA CAGCAGCGCA 
GTTCGTCGGG ACTCACTTTT GGCCAGTTCA GAGCTGTATA AGCGGGGTGG CGGTGGCAGC 
TTGACTCCCA TCGGCCAGTC TTTTTACAAC AGTCTTGGAT ACTCTTCCTC TCCCAGCCCC 
ATTGGACTGA CACCTGGTCA CTCACCTCTG ACACCTCCAC CCTCTTTACC CTCCTCGCAT 
GGATCCTCAT CCAGTATCCA CCTAGGTGGT TTGCCCAATG GCAGTGGCCG ATATATTTCT 
GCTGCTCCAG GAGCAGAAGC CAAATACAGA AGCTCGGGCA GTACCTCCAG TCTTTTTAGC 
TCTAGCAGTC AACTTTTCCC CCCTCCACGA GCTCGATACA GCCGATCAGA TGTCATGCCT 
TCAGGTCGCA GCCGCTTACT AGAGGATTTC CGAAATAACC GTTTTCCTAA CCTGCAACTA 
CGAGACTTGC CTGGACACAT GGTGGAGTTC TCTCAAGATC AGCATGGGTC CAGGTTTATC 
CAGCAAAAGT TGGAGCGTGC AACATCTGCA GAGAGACAGA TGGTGTTTGG GGAAATTCTA 
CAAGCTGCTT ACCAACTCAT GACTGATGTA TTTGGCAACT ATGTCATACA GAAGTTCTTT 
GAGTTTGGCA GTGCAGATCA GAAACTAGCT CTGGCGACTC GTATCCGTGG CCATGTGCTG 
CCACTTGCTC TGCAGATGTA TGGTTGTCGT GTGATCCAGA AAGCTCTGGA GTCCATCTCC 
TCTGACCAGC AGGTAATTAG TGACATTGTC CGAGAGTTGG ATGGCCATGT TCTGAAGTGT 
GTGAAGGATC AGAATGGAAA TCACGTGGTG CGGAAATGTA TTGAGTGTGT CCAGCCCCAG 
GCTCTTCAGT TCATCATTGA TGCCTTTCAG GGCCAGGTAT TTGTTTTGTC CACTCATCCA 
TATGGCTGCA GAGTGATACA GCGTATCCTG GAGCACTGTA CTCAGGAACA AACCTTGCCC 
ATCCTGGAAG AACTCCACCA GCACTCTGAG CAGTTGGGAC AGAAGTATCA AGGTGTTTCA 
TTGGAGATGA CCCCCAAAAC ATATTATACA GTGTCCAGCG ATGCACTGTT CAAGGATCAG 
TATGGTAACT ATGTTATCCA GCATGTTTTG GAGCATGGGC GACCAGAAGA TAAGAGTAAG 
ATAGTGGCGG AGGTCAGAGG GAAGGTTCTG GCTCTGAGTC AGCATAAATC GTTGCAAGCA 
ATGTTGTGGA GAAGTGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096040.1
CDS	109..3429
Translation	MSIPCSILGMNDVPWQETQCGTLHTNGAPETGGVRVHAGCPLATVGGARQVPGGVHPLQGMANPNPCTQPLLSGRSQDDATVGYFFQRQPGEQMGGCTGNKHRWPTGDGNHVDQLRSVDEMNYNFQALALESRGMGELLPAKKLWESDELAKDGRKAMLVGEEWRENAWSSSHHALSQPIMVQKRPGQGYNGTGETSSVLSPRSEGGGLGVSMVEYVLSSSPGDKMDGRYRNGGYGEADADPDGREKGESKEKSSPFEENKSPEMKVGDEGDTSKANARGLLNGMDRDCKDFNPTPGSRQASPTEAVERLGPSQAGLEIMVQHHQHTLQAPNPVTNKPPPEDFQSQEAQNMGGMEQQAGLEALQFDYGGNQIQLDSSGAAVGLFNYDSQQQLFQRSSHLTVQQLNAAQQQQYALAAAQQQHLGLAPAFVPNPYIINAAPPGADPYTAAGLAAAATLAGPAVVPPQYYGVPWGVYPANLFQQQATAAASHSANQQASSQGPGQQVIRAGSNQRPLTPGQSQQSQQESLAAAATNPTLAFPGMSGYQVLAPAAYYDQTGALVMGPGTRTGLGGPVRLVQTPLLINPAAAQAAAAASVSGSGNSMSGPANGLYRSMACQQPQQPQPQQQLPPPSSGLPSNSFYGSGSVAPSSQSSSLFSHTSAAPPPSTSLGFSSTSGSLGVGLGSALGAFGSSVSSSTNSSAVRRDSLLASSELYKRGGGGSLTPIGQSFYNSLGYSSSPSPIGLTPGHSPLTPPPSLPSSHGSSSSIHLGGLPNGSGRYISAAPGAEAKYRSSGSTSSLFSSSSQLFPPPRARYSRSDVMPSGRSRLLEDFRNNRFPNLQLRDLPGHMVEFSQDQHGSRFIQQKLERATSAERQMVFGEILQAAYQLMTDVFGNYVIQKFFEFGSADQKLALATRIRGHVLPLALQMYGCRVIQKALESISSDQQVISDIVRELDGHVLKCVKDQNGNHVVRKCIECVQPQALQFIIDAFQGQVFVLSTHPYGCRVIQRILEHCTQEQTLPILEELHQHSEQLGQKYQGVSLEMTPKTYYTVSSDALFKDQYGNYVIQHVLEHGRPEDKSKIVAEVRGKVLALSQHKSLQAMLWRSV

Target ORF information:

RefSeq Version	NM_001102570.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pumilio RNA-binding family member 2 (pum2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001102570.1

ORF Insert Sequence:

ATGAGCATTC CATGCAGCAT CCTAGGTATG AATGACGTAC CCTGGCAGGA GACACAATGT 
GGAACGCTGC ACACCAATGG TGCACCAGAA ACTGGTGGGG TCAGAGTCCA TGCAGGGTGT 
CCTCTAGCCA CGGTTGGAGG TGCCAGGCAG GTACCTGGAG GAGTTCACCC TTTGCAGGGA 
ATGGCCAATC CCAACCCCTG CACCCAGCCT CTTCTGAGTG GTCGTTCACA AGATGATGCC 
ACTGTGGGCT ATTTCTTTCA AAGGCAGCCT GGAGAGCAAA TGGGAGGCTG CACTGGCAAC 
AAACACCGCT GGCCCACTGG TGATGGAAAC CATGTTGACC AGTTGCGCTC TGTGGATGAG 
ATGAACTATA ACTTTCAAGC ATTGGCTCTG GAGTCTCGCG GAATGGGTGA ACTACTTCCT 
GCAAAAAAAT TGTGGGAATC AGATGAATTG GCAAAAGATG GACGTAAAGC AATGCTTGTG 
GGTGAAGAAT GGAGAGAGAA TGCCTGGAGT TCTTCTCATC ATGCTCTCTC TCAACCCATT 
ATGGTGCAGA AGCGACCAGG CCAAGGCTAC AATGGGACTG GAGAGACAAG CTCAGTGCTG 
TCCCCACGAT CAGAGGGGGG TGGTCTTGGT GTGAGTATGG TGGAGTATGT CCTCAGCTCC 
TCCCCTGGAG ACAAGATGGA TGGTCGCTAC AGGAATGGAG GATATGGTGA AGCAGATGCA 
GATCCAGATG GAAGAGAAAA GGGAGAATCA AAAGAGAAGT CTTCACCTTT CGAAGAAAAC 
AAAAGTCCTG AGATGAAAGT TGGAGATGAA GGGGATACCT CAAAAGCAAA TGCCAGGGGA 
TTACTCAATG GAATGGACCG AGATTGCAAA GACTTCAACC CCACTCCAGG TAGTCGCCAG 
GCATCCCCCA CAGAAGCAGT AGAACGACTG GGACCCAGTC AGGCTGGGCT GGAAATAATG 
GTGCAACACC ACCAGCACAC TCTGCAGGCA CCCAACCCTG TTACAAACAA ACCTCCTCCT 
GAAGACTTCC AGAGTCAGGA AGCCCAGAAC ATGGGTGGCA TGGAGCAGCA GGCGGGGCTG 
GAGGCTCTTC AGTTTGACTA TGGTGGAAAT CAGATCCAGT TGGACTCATC AGGTGCTGCA 
GTCGGCCTAT TTAACTACGA CTCTCAACAG CAGTTGTTCC AGAGATCCAG TCATCTCACA 
GTTCAGCAGC TGAATGCAGC ACAGCAACAA CAGTACGCCC TTGCTGCAGC CCAACAGCAA 
CACCTTGGCC TTGCACCTGC ATTTGTGCCA AACCCTTACA TCATCAATGC TGCCCCTCCT 
GGAGCAGACC CCTACACTGC TGCAGGATTA GCAGCAGCTG CAACACTTGC AGGTCCAGCT 
GTAGTTCCGC CCCAGTACTA TGGGGTCCCT TGGGGTGTGT ATCCAGCCAA TCTCTTCCAA 
CAACAAGCTA CAGCTGCTGC CAGTCATTCT GCTAACCAGC AGGCATCCAG TCAGGGTCCA 
GGGCAACAGG TGATTCGAGC AGGGTCCAAT CAACGACCTT TAACTCCAGG ACAGAGCCAA 
CAGAGTCAGC AGGAGTCGCT TGCTGCAGCT GCCACAAACC CAACACTGGC CTTTCCAGGC 
ATGTCAGGCT ACCAGGTCCT AGCGCCGGCT GCTTATTATG ATCAGACTGG TGCATTGGTC 
ATGGGTCCTG GAACTCGCAC TGGCCTGGGT GGACCTGTTC GACTGGTCCA AACCCCTCTT 
CTCATCAACC CAGCAGCCGC ACAAGCTGCT GCAGCTGCAT CTGTATCAGG CTCTGGTAAT 
AGTATGTCTG GTCCTGCTAA TGGTCTATAC CGGTCCATGG CATGCCAACA ACCCCAGCAG 
CCTCAACCTC AGCAGCAGCT TCCTCCACCT TCCAGCGGTC TGCCTTCAAA CTCCTTCTAT 
GGCTCTGGTT CTGTAGCACC CAGTTCCCAG AGCAGCTCAC TTTTCTCCCA TACAAGTGCA 
GCTCCTCCAC CTAGCACCTC TCTTGGTTTC AGCAGCACTA GTGGATCTTT AGGTGTTGGA 
TTAGGCTCTG CTCTTGGTGC ATTTGGCTCC TCAGTGTCCA GTTCCACTAA CAGCAGCGCA 
GTTCGTCGGG ACTCACTTTT GGCCAGTTCA GAGCTGTATA AGCGGGGTGG CGGTGGCAGC 
TTGACTCCCA TCGGCCAGTC TTTTTACAAC AGTCTTGGAT ACTCTTCCTC TCCCAGCCCC 
ATTGGACTGA CACCTGGTCA CTCACCTCTG ACACCTCCAC CCTCTTTACC CTCCTCGCAT 
GGATCCTCAT CCAGTATCCA CCTAGGTGGT TTGCCCAATG GCAGTGGCCG ATATATTTCT 
GCTGCTCCAG GAGCAGAAGC CAAATACAGA AGCTCGGGCA GTACCTCCAG TCTTTTTAGC 
TCTAGCAGTC AACTTTTCCC CCCTCCACGA GCTCGATACA GCCGATCAGA TGTCATGCCT 
TCAGGTCGCA GCCGCTTACT AGAGGATTTC CGAAATAACC GTTTTCCTAA CCTGCAACTA 
CGAGACTTGC CTGGACACAT GGTGGAGTTC TCTCAAGATC AGCATGGGTC CAGGTTTATC 
CAGCAAAAGT TGGAGCGTGC AACATCTGCA GAGAGACAGA TGGTGTTTGG GGAAATTCTA 
CAAGCTGCTT ACCAACTCAT GACTGATGTA TTTGGCAACT ATGTCATACA GAAGTTCTTT 
GAGTTTGGCA GTGCAGATCA GAAACTAGCT CTGGCGACTC GTATCCGTGG CCATGTGCTG 
CCACTTGCTC TGCAGATGTA TGGTTGTCGT GTGATCCAGA AAGCTCTGGA GTCCATCTCC 
TCTGACCAGC AGGTAATTAG TGACATTGTC CGAGAGTTGG ATGGCCATGT TCTGAAGTGT 
GTGAAGGATC AGAATGGAAA TCACGTGGTG CGGAAATGTA TTGAGTGTGT CCAGCCCCAG 
GCTCTTCAGT TCATCATTGA TGCCTTTCAG GGCCAGGTAT TTGTTTTGTC CACTCATCCA 
TATGGCTGCA GAGTGATACA GCGTATCCTG GAGCACTGTA CTCAGGAACA AACCTTGCCC 
ATCCTGGAAG AACTCCACCA GCACTCTGAG CAGTTGGGAC AGAAGTATCA AGGTGTTTCA 
TTGGAGATGA CCCCCAAAAC ATATTATACA GTGTCCAGCG ATGCACTGTT CAAGGATCAG 
TATGGTAACT ATGTTATCCA GCATGTTTTG GAGCATGGGC GACCAGAAGA TAAGAGTAAG 
ATAGTGGCGG AGGTCAGAGG GAAGGTTCTG GCTCTGAGTC AGCATAAATC GTTGCAAGCA 
ATGTTGTGGA GAAGTGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Extensive alternative polyadenylation during zebrafish development.
Genome research22(10)2054-66(2012 Oct)
Ulitsky I,Shkumatava A,Jan CH,Subtelny AO,Koppstein D,Bell GW,Sive H,Bartel DP

Identification and characterization of the pumilio-2 expressed in zebrafish embryos and adult tissues.
Molecular biology reports39(3)2811-9(2012 Mar)
Wang HN,Xu Y,Tao LJ,Zhou J,Qiu MX,Teng YH,Deng FJ