tert cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tert
Entrez Gene ID	796551
Full Name	telomerase reverse transcriptase
General protein information	Preferred Names telomerase reverse transcriptase Names telomerase reverse transcriptase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009293924.2	XP_009292199.1	telomerase reverse transcriptase isoform X1
NM_001083866.1	NP_001077335.1	telomerase reverse transcriptase

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TERT	NP_001026800.1
Gallus gallus (chicken)	TERT	NP_001026178.1
Mus musculus (house mouse)	Tert	NP_033380.1
Rattus norvegicus (Norway rat)	Tert	NP_445875.1
Xenopus tropicalis (tropical clawed frog)	tert	XP_004915405.1
Bos taurus (cattle)	TERT	NP_001039707.1
Danio rerio (zebrafish)	tert	NP_001077335.1
Homo sapiens (human)	TERT	NP_937983.2
Pan troglodytes (chimpanzee)	TERT	XP_003950543.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Short Telomeres in Key Tissues Initiate Local and Systemic Aging in Zebrafish.
Carneiro MC, Henriques CM, Nabais J, Ferreira T, Carvalho T, Ferreira MG
PLoS genetics12(1)e1005798(2016 Jan)

DNA methylation of genes of the main components of the telomerase complex in Danio rerio.
Belova EV, Kozlov AE, Shubernetskaya OS, Zvereva MI, Shpanchenko OV, Dontsova OA
Doklady. Biochemistry and biophysics464329-32(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Telomerase is essential for heart muscle regeneration in zebrafish.
Title: Telomerase Is Essential for Zebrafish Heart Regeneration.

methylation status of the genes of telomerase reverse transcriptase (tert) and telomerase RNA (terc) was determined in brain tissues; study found that, regardless of the age of fish, the regulatory region of the tert gene was completely methylated, whereas the coding region remained unmethylated
Title: DNA methylation of genes of the main components of the telomerase complex in Danio rerio.

Telomerase-deficient zebrafish show p53-dependent premature aging and reduced lifespan in the first generation.
Title: Premature aging in telomerase-deficient zebrafish.

Thus, telomerase is limiting for zebrafish lifespan, enabling the study of telomere shortening in naturally ageing individuals.
Title: Telomerase is required for zebrafish lifespan.

a direct relationship between the expression of telomerase, telomere length and the efficiency of tissue regeneration
Title: Behaviour of telomere and telomerase during aging and regeneration in zebrafish.

The following tert gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tert cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01322	NM_001083866.1 Latest version!	Danio rerio telomerase reverse transcriptase (tert), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
ODa47086	XM_009293924.2 Latest version!	Danio rerio telomerase reverse transcriptase (tert), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tert ( NM_001083866.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001077335.1 Danio rerio telomerase reverse transcriptase (tert), mRNA.

Price & Availability↑

CloneID	ODa01322
Clone ID Related Accession (Same CDS sequence)	NM_001083866.1
Accession Version	NM_001083866.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-20
Organism	Danio rerio(zebrafish)
Product	telomerase reverse transcriptase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF202140.1. On Apr 5, 2007 this sequence version replaced XM_001336868.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF202140.1, AB458561.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCTGGAC AGTACTCGAC AGATGGCGGA TTTAGGCCGG TTTTGGAGAT TCTGCGCTCC 
TTATATCCGG TCGTGCAGAC TTTGGAGGAG TTCACCGACG GACTGCAATT CCCTGACGGC 
CGAAAGCCGG TTCTGCTGGA GGAAACAGAC GGCGCGCGCT TTAAAAAGCT CCTCAGTGGA 
CTTATTGTAT GTGCGTACAC GCCGCCGCAG CTGCGCGTCC CCGCCCAGCT CAGCACCCTG 
CCGGAGGTCT TGGCGTTCAC TCTGAACCAC ATTAAACGTA AGAAACTGAG GAACGTCCTG 
GGCTTCGGTT ATCAATGCAG CGACGTGACG ACCAGTTCGG ATCCCTTCCG TTTCCATGGC 
GACGTTTCGC AGACGGCTGC CTCCATCAGC ACCAGCGAGG TCTGGAAGCG TATCAACCAG 
CGTCTGGGCA CGGAGGTAAC GCGGTACCTG CTGCAGGACT GTGCCGTTTT CACCACCGTC 
CCGCCATCGT GTGTTCTGCA GGTGTGCGGA GAACCTGTTT ACGACTTGCT GATGCCGCGC 
TCATGGTCTG GCTTTTTCCT CAGTAACTCA GATAATGAAC GAATCAGCGG CGCGATGCGG 
AAATTCCCTG CTGTCCAGAA GACAGTCGCA ATTTCCAAAA AGAGAACAAG AGATAACGAA 
AAATATATTT CGGTAAAGCG GCGGAGGGTA AAGGAAACTG TGAATAATAA TAACGGAAAT 
TACAGATCTC TGTGTTTTGC AATTTCTAAA AAGAGAGCGA TAGATAATGA AGAAAATATT 
TCGTTAAAGC GACGGAGGAT GGAGGAAACT GACCAAGTAG CGAAAATACG TAATGAAAAT 
CACGAATCTC AGAGTTTCGC AATTTCTAAA AAGAGAGCGA GAGATAATGA AGAAAATATT 
TCGTTAAAGC GACAAAGGAT GGAGGAAATT GACCAAGTAG CGAAAATACG TAACGAAAAT 
CATGGATCTC AGAGTTGGAA ACCAGCAGAT CAGCGTCCTC CTCGACCCTC GCAATGTTCA 
ATACGCGTTC TGAGCATGCT CTACAATGGG CGGGGCATGA AGAACTTCCT GCTCAACAGG 
AAGTTGAAAG GAGTGGGCGG GGCCAGGCGC ATGCAAGGGG AGGATCTTGT CCGCATGATT 
TTCCTCCAAT CAGAATCCAA CGACAGCAAA CCGAAAAAAC TTCCCAAACG ATTCTTCGCA 
ATGGTGCCGC TATTCAGTCG GCTGTTGCGG CAGCACAGGA AGTGTCCGTA TCGGCTGTTC 
CTGCAGAGGA AGTGTGCAGG AAATCCAGAC GTGAAGGATA TGGAGTCTCT GCTGAAGTCA 
CACTCGTCTC CATATAGAGT TTATCTGTTC GTCAGGGAGT GTCTGCGCCA TATTATTCCC 
CACGAGCTCT GGGGCTGCCA GGAAAACCAG CTCCACTTCC TGTCTAATGT AAAGAACTTC 
CTGCTTCTGG GGAAGTTTGA GCGCCTCACG CTGGTCCAGC TGATGTGGAG GATGAAGGTT 
CAGGCCTGCC ATTGGCTGGG GCCCAAGAAA CGTCAGTGTG CGAGCGAGCA CCGCTACCGT 
GAGTGGATGT TGGGTCAGTG TATGGGCTGG ATGTTGAGTG GTTTTGTGGT CGGTCTGGTC 
AGAGCTCAGT TCTACATCAC GGAGAGTATG GGCCACAAAC ACACACTGCG CTTCTACAGG 
GGAGATGTCT GGAGCAGACT GCAGGACCAG GCCTTCAGGG CTCATCTGTG TAAGGGCCAG 
TGGAGGCCCC TGTCTCCATC CCAGGCGCTG AAGGTCCCCA ATAGTGCAGT GACATCCCGC 
ATCCGCTTTA TTCCCAAAAC CAGCAGCATG AGGCCCATCA CACGCCTCAG CGGCAGCAGA 
GACACACTGC AGTATTTTCA GAGCTGTGTG CGTGTGCTGC AGAATGTGTT GAGTGTGTGT 
GTGCGTGAGG CCCCGGGGCC CATGGGCTCC ACCGTCTGGG GTTGGCAGGA CATTCACAGA 
CGCCTGCAAG ACTTCAGCCC TCAGCAGAAG AGCTCGCCAC GACCGCTCTA CTTCGTCAAG 
GTGGATGTGA GCGGAGCGTA TGACAGTCTC CCGCACCTGA AGCTGGTGGA GGTGCTGAAG 
GAAGTGTTGG GTCCGTTTGC AGAGCAGAGC TTCTTCCTGC GTCAGTACAG CAGTGTGTGG 
AGCGACCCGA CCCGCGGCCT GCGCAAACGC TTCTGCACCA AAGCTGAGAT GTCAGAGCCG 
CTCAACATGA AGGGGTTTGT TGTGGATGAA CAGGTCAGCG GGCGCCTGCA TGACGCTATA 
TTAGTGGAGC GGCACTCGTC TGAGGTCAGA GGTGGAGACG TCTTCCAGTT CTTCCAGAAG 
ATGCTCTGCA GTTACGTCAT CCATTACGAC CAGCAGATGT TCCGGCAGGT GTGTGGGATC 
CCGCAGGGCT CTTCAGTGTC TTCTCTGCTG TGTAATCTGT GTTACGGACA CATGGAGAAA 
GCCCTGCTGA AGGACATCGC TAAAGGAGGG TGTCTGATGA GGCTGATTGA TGATTTTTTG 
CTCATTACTC CTCATCTGAG TAAAGCCACA GAGTTCCTGA CCACTCTTCT GTCTGGAGTT 
CCAGATTACG GTTGCCAGAT TAACCCTCAG AAGGTGGCGG TGAACTTCCC CGTGTGTGTG 
TCCTGGGTAA ACTCGGGCGT CTCTGTGCTG CCGTCCAGCT GCCTGTTCCC CTGGTGCGGC 
TTGATGATAC ACACACACAC GCTGGACGTC TATAAAGACT ACTCACGGTA TGACGGCCTA 
TCACTGCGCT ACAGCCTGAC TCTTGGCTCC GCCCACTCTC CATCTACAGT CATGAAGAAG 
CTGCTGTCGG TGCTCAGCAT CAAAAGCACG GACATCTTCT TAGACCTCAG GCTGAACTCT 
GTGGAGGCCG TTTACAGGAG TCTGTATAAG CTGATTCTGC TGCAGGCGCT CAGGTTTCAT 
GCGTGCGTGA GGAGTCTGCC GTTGGGTCAG AGTGTGAACA GAAACCCGTC GTTCTTCCTG 
AAGATGATCT GGAGAATGAC TCGAGTCACC AATAAACTCC TCACACACAT TAACAAAGGT 
CTGCCTGTGT GTTCTGTGGA CAGTGGTGGT GTTCTGCAGT CTGAGGCGGT TCAGCTTTTA 
TTCTGTTTGG CCTTCGAGAC GCTTTTCAGA CGGTTTCGCT CGGTTTACCA CTGCCTGATC 
CCTGCACTGC ACAAACGGAA GCGTGCTCTT CAGCGTGAGC TCTGCGGGAT CACTCTGGCT 
CGGGTCCGTC AAGCTTCCTC TCCCAGAATC CCCCTGGATT TCAGCATGCG GGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077335.1
CDS	29..3325
Translation	MSGQYSTDGGFRPVLEILRSLYPVVQTLEEFTDGLQFPDGRKPVLLEETDGARFKKLLSGLIVCAYTPPQLRVPAQLSTLPEVLAFTLNHIKRKKLRNVLGFGYQCSDVTTSSDPFRFHGDVSQTAASISTSEVWKRINQRLGTEVTRYLLQDCAVFTTVPPSCVLQVCGEPVYDLLMPRSWSGFFLSNSDNERISGAMRKFPAVQKTVAISKKRTRDNEKYISVKRRRVKETVNNNNGNYRSLCFAISKKRAIDNEENISLKRRRMEETDQVAKIRNENHESQSFAISKKRARDNEENISLKRQRMEEIDQVAKIRNENHGSQSWKPADQRPPRPSQCSIRVLSMLYNGRGMKNFLLNRKLKGVGGARRMQGEDLVRMIFLQSESNDSKPKKLPKRFFAMVPLFSRLLRQHRKCPYRLFLQRKCAGNPDVKDMESLLKSHSSPYRVYLFVRECLRHIIPHELWGCQENQLHFLSNVKNFLLLGKFERLTLVQLMWRMKVQACHWLGPKKRQCASEHRYREWMLGQCMGWMLSGFVVGLVRAQFYITESMGHKHTLRFYRGDVWSRLQDQAFRAHLCKGQWRPLSPSQALKVPNSAVTSRIRFIPKTSSMRPITRLSGSRDTLQYFQSCVRVLQNVLSVCVREAPGPMGSTVWGWQDIHRRLQDFSPQQKSSPRPLYFVKVDVSGAYDSLPHLKLVEVLKEVLGPFAEQSFFLRQYSSVWSDPTRGLRKRFCTKAEMSEPLNMKGFVVDEQVSGRLHDAILVERHSSEVRGGDVFQFFQKMLCSYVIHYDQQMFRQVCGIPQGSSVSSLLCNLCYGHMEKALLKDIAKGGCLMRLIDDFLLITPHLSKATEFLTTLLSGVPDYGCQINPQKVAVNFPVCVSWVNSGVSVLPSSCLFPWCGLMIHTHTLDVYKDYSRYDGLSLRYSLTLGSAHSPSTVMKKLLSVLSIKSTDIFLDLRLNSVEAVYRSLYKLILLQALRFHACVRSLPLGQSVNRNPSFFLKMIWRMTRVTNKLLTHINKGLPVCSVDSGGVLQSEAVQLLFCLAFETLFRRFRSVYHCLIPALHKRKRALQRELCGITLARVRQASSPRIPLDFSMRV

Target ORF information:

RefSeq Version	NM_001083866.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio telomerase reverse transcriptase (tert), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083866.1

ORF Insert Sequence:

ATGTCTGGAC AGTACTCGAC AGATGGCGGA TTTAGGCCGG TTTTGGAGAT TCTGCGCTCC 
TTATATCCGG TCGTGCAGAC TTTGGAGGAG TTCACCGACG GACTGCAATT CCCTGACGGC 
CGAAAGCCGG TTCTGCTGGA GGAAACAGAC GGCGCGCGCT TTAAAAAGCT CCTCAGTGGA 
CTTATTGTAT GTGCGTACAC GCCGCCGCAG CTGCGCGTCC CCGCCCAGCT CAGCACCCTG 
CCGGAGGTCT TGGCGTTCAC TCTGAACCAC ATTAAACGTA AGAAACTGAG GAACGTCCTG 
GGCTTCGGTT ATCAATGCAG CGACGTGACG ACCAGTTCGG ATCCCTTCCG TTTCCATGGC 
GACGTTTCGC AGACGGCTGC CTCCATCAGC ACCAGCGAGG TCTGGAAGCG TATCAACCAG 
CGTCTGGGCA CGGAGGTAAC GCGGTACCTG CTGCAGGACT GTGCCGTTTT CACCACCGTC 
CCGCCATCGT GTGTTCTGCA GGTGTGCGGA GAACCTGTTT ACGACTTGCT GATGCCGCGC 
TCATGGTCTG GCTTTTTCCT CAGTAACTCA GATAATGAAC GAATCAGCGG CGCGATGCGG 
AAATTCCCTG CTGTCCAGAA GACAGTCGCA ATTTCCAAAA AGAGAACAAG AGATAACGAA 
AAATATATTT CGGTAAAGCG GCGGAGGGTA AAGGAAACTG TGAATAATAA TAACGGAAAT 
TACAGATCTC TGTGTTTTGC AATTTCTAAA AAGAGAGCGA TAGATAATGA AGAAAATATT 
TCGTTAAAGC GACGGAGGAT GGAGGAAACT GACCAAGTAG CGAAAATACG TAATGAAAAT 
CACGAATCTC AGAGTTTCGC AATTTCTAAA AAGAGAGCGA GAGATAATGA AGAAAATATT 
TCGTTAAAGC GACAAAGGAT GGAGGAAATT GACCAAGTAG CGAAAATACG TAACGAAAAT 
CATGGATCTC AGAGTTGGAA ACCAGCAGAT CAGCGTCCTC CTCGACCCTC GCAATGTTCA 
ATACGCGTTC TGAGCATGCT CTACAATGGG CGGGGCATGA AGAACTTCCT GCTCAACAGG 
AAGTTGAAAG GAGTGGGCGG GGCCAGGCGC ATGCAAGGGG AGGATCTTGT CCGCATGATT 
TTCCTCCAAT CAGAATCCAA CGACAGCAAA CCGAAAAAAC TTCCCAAACG ATTCTTCGCA 
ATGGTGCCGC TATTCAGTCG GCTGTTGCGG CAGCACAGGA AGTGTCCGTA TCGGCTGTTC 
CTGCAGAGGA AGTGTGCAGG AAATCCAGAC GTGAAGGATA TGGAGTCTCT GCTGAAGTCA 
CACTCGTCTC CATATAGAGT TTATCTGTTC GTCAGGGAGT GTCTGCGCCA TATTATTCCC 
CACGAGCTCT GGGGCTGCCA GGAAAACCAG CTCCACTTCC TGTCTAATGT AAAGAACTTC 
CTGCTTCTGG GGAAGTTTGA GCGCCTCACG CTGGTCCAGC TGATGTGGAG GATGAAGGTT 
CAGGCCTGCC ATTGGCTGGG GCCCAAGAAA CGTCAGTGTG CGAGCGAGCA CCGCTACCGT 
GAGTGGATGT TGGGTCAGTG TATGGGCTGG ATGTTGAGTG GTTTTGTGGT CGGTCTGGTC 
AGAGCTCAGT TCTACATCAC GGAGAGTATG GGCCACAAAC ACACACTGCG CTTCTACAGG 
GGAGATGTCT GGAGCAGACT GCAGGACCAG GCCTTCAGGG CTCATCTGTG TAAGGGCCAG 
TGGAGGCCCC TGTCTCCATC CCAGGCGCTG AAGGTCCCCA ATAGTGCAGT GACATCCCGC 
ATCCGCTTTA TTCCCAAAAC CAGCAGCATG AGGCCCATCA CACGCCTCAG CGGCAGCAGA 
GACACACTGC AGTATTTTCA GAGCTGTGTG CGTGTGCTGC AGAATGTGTT GAGTGTGTGT 
GTGCGTGAGG CCCCGGGGCC CATGGGCTCC ACCGTCTGGG GTTGGCAGGA CATTCACAGA 
CGCCTGCAAG ACTTCAGCCC TCAGCAGAAG AGCTCGCCAC GACCGCTCTA CTTCGTCAAG 
GTGGATGTGA GCGGAGCGTA TGACAGTCTC CCGCACCTGA AGCTGGTGGA GGTGCTGAAG 
GAAGTGTTGG GTCCGTTTGC AGAGCAGAGC TTCTTCCTGC GTCAGTACAG CAGTGTGTGG 
AGCGACCCGA CCCGCGGCCT GCGCAAACGC TTCTGCACCA AAGCTGAGAT GTCAGAGCCG 
CTCAACATGA AGGGGTTTGT TGTGGATGAA CAGGTCAGCG GGCGCCTGCA TGACGCTATA 
TTAGTGGAGC GGCACTCGTC TGAGGTCAGA GGTGGAGACG TCTTCCAGTT CTTCCAGAAG 
ATGCTCTGCA GTTACGTCAT CCATTACGAC CAGCAGATGT TCCGGCAGGT GTGTGGGATC 
CCGCAGGGCT CTTCAGTGTC TTCTCTGCTG TGTAATCTGT GTTACGGACA CATGGAGAAA 
GCCCTGCTGA AGGACATCGC TAAAGGAGGG TGTCTGATGA GGCTGATTGA TGATTTTTTG 
CTCATTACTC CTCATCTGAG TAAAGCCACA GAGTTCCTGA CCACTCTTCT GTCTGGAGTT 
CCAGATTACG GTTGCCAGAT TAACCCTCAG AAGGTGGCGG TGAACTTCCC CGTGTGTGTG 
TCCTGGGTAA ACTCGGGCGT CTCTGTGCTG CCGTCCAGCT GCCTGTTCCC CTGGTGCGGC 
TTGATGATAC ACACACACAC GCTGGACGTC TATAAAGACT ACTCACGGTA TGACGGCCTA 
TCACTGCGCT ACAGCCTGAC TCTTGGCTCC GCCCACTCTC CATCTACAGT CATGAAGAAG 
CTGCTGTCGG TGCTCAGCAT CAAAAGCACG GACATCTTCT TAGACCTCAG GCTGAACTCT 
GTGGAGGCCG TTTACAGGAG TCTGTATAAG CTGATTCTGC TGCAGGCGCT CAGGTTTCAT 
GCGTGCGTGA GGAGTCTGCC GTTGGGTCAG AGTGTGAACA GAAACCCGTC GTTCTTCCTG 
AAGATGATCT GGAGAATGAC TCGAGTCACC AATAAACTCC TCACACACAT TAACAAAGGT 
CTGCCTGTGT GTTCTGTGGA CAGTGGTGGT GTTCTGCAGT CTGAGGCGGT TCAGCTTTTA 
TTCTGTTTGG CCTTCGAGAC GCTTTTCAGA CGGTTTCGCT CGGTTTACCA CTGCCTGATC 
CCTGCACTGC ACAAACGGAA GCGTGCTCTT CAGCGTGAGC TCTGCGGGAT CACTCTGGCT 
CGGGTCCGTC AAGCTTCCTC TCCCAGAATC CCCCTGGATT TCAGCATGCG GGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tert ( XM_009293924.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292199.1 Danio rerio telomerase reverse transcriptase (tert), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa47086
Clone ID Related Accession (Same CDS sequence)	XM_009293924.2
Accession Version	XM_009293924.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	telomerase reverse transcriptase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293924.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAC AGTACTCGAC AGATGGCGGA TTCAGGCCGG TTTTGGAGAT TCTGCGCTCC 
TTATATCCAG TCGTGCAGAC TTTGGAGGAG TTCACCGACG GACTGCAATT CCCTGACGGC 
CGAAAGCCGG TTCTGCTGGA GGAAACAGAC GGCGCGCGCT TTAAAAAGCT CCTCAGTGGA 
CTTATTGTAT GCGCCTACAC GCCGCCGCAG CTGCGCGTCC CCGCCCAGCT CAGCACCCTG 
CCGGAGGTCT TGGCGTTCAC TCTGAACCAC ATTAAACGTA AGAAACTGAG GAACGTCCTG 
GGCTTCGGTT ATCAATGCAG CGACGTGACG ACCAGTTCGG ATCCCTTCCG TTTCCATGGT 
GACGTTTCGC AGACGGCTGC TTCCATCAGC ACCAGCGAGG TCTGGAAGCG TATCAACCAG 
CGTCTGGGCA CGGAGGTAAC GCGGTACCTG CTGCAGGACT GTGCCGTTTT CACCACCGTC 
CCGCCATCGT GTGTTCTGCA GGTGTGCGGA GAACCTGTTT ACGACTTGCT GATGCCGCGC 
TCATGGTCTG GCTTTTTCCT CAGTAACTCA GATAATGAAC GAATCAGCGG CGCGATGCGG 
AAATCCCCTG CTGTCCAGAA GACAGTCGCA ATTTCCAAAA AGAGAACAAG AGATAACGAA 
AAATATATTT CGGTAAAGCG GCGGAGGGTA AAGGAAACTG TGAATAATAA TAACGGAAAT 
TACAGATCTC GGTGTTTTGC AATTTCTAAA AAGAGAGCGA GAGATAATGA AGAAAATATT 
TCGTTAAAGC GACGGAGGAT GGAGGAAACT GACCAAGAAG CGAAAATACG TAATGAAAAT 
CACGGATCTC AGAGTTTCAC AATTTCTAAA AAGAGAGCGA GAGATAACGA AGAAAATATT 
TCGTTAAAGC GACAAAGGAT GGAGGAAATT GACCAAGTAG CGAAAATACG TAACGAAAAT 
CACGGATCTC AGAGTTGGAA ACCAGCGGAT CAGCGTCCTC CTCGACCCTC GCAATGTTCA 
ATACGCGTTC TGAGCATGCT CTACAATGGG CGGGGCATGA AGAACTTCCT GCTCAACAGG 
AAGTTGAAAG GAGTGGGCGG GGCCAGGCGC ATGCAAGGGG AGGATCTTGT CCGCATGATT 
TTCCTCCAAT CAGAATCCAA CGATAGCAAA CCGAAAAAAC TTCCTAAACG ATTCTTCGCA 
ATGGTGCCGC TATTCAGTCG GCTGTTGCGG CAGCACAGGA AGTGTCCGTA TCGGCTGTTC 
CTGCAAAGAA AGTGTGCAGG AAATCCAGAC GTGGAGGATA TGGAGTCTCT GCTGAAGTCA 
CACTCGTCTC CATATAGAGT TTATCTGTTC GTCAGGGAGT GTCTGCGCCA TATTATTCCC 
CACGAGCTCT GGGGCTGCCA GGAAAACCAG CTCCACTTCC TGTCTAATGT AAAGAACTTC 
CTGCTTCTGG GGAAGTTTGA GCGCCTCACG CTGGTCCAGC TGATGTGGAG GATGAAGGTT 
CAGGCCTGCC ATTGGCTGGG GCCCAAGAAA CGTCAGTGTG CGAGCGAGCA CCGCTACCGT 
GAGTGGATGT TGGGTCAGTG TATGGGCTGG GTGTTGAGTG GTTTTGTGGT CGGTCTGGTC 
AGAGCTCAGT TCTACATCAC GGAGAGTATG GGCCACAAAC ACACACTGCG CTTCTACAGG 
GGAGATGTCT GGAGCAGACT GCAGGACCAG GCCTTCAGGG CTCATCTGTG TAAGGGCCAG 
TGGAGGCCCC TGTCTCCATC CCAGGCGCTG AAGGTCCCCA ATAGTGCAGT GACATCCCGC 
ATCCGCTTTA TTCCCAAAAC CAGCAGCATG AGGCCCATCA CACGCCTCAG CGGCAGCAGA 
GACACACTGC AGTATTTTCA GAGCTGTGTG CGTGTGCTGC AGAATGTGTT GAGTGTGTGT 
GTGCGTGAGG CCCCGGGGCC CATGGGCTCC ACCGTCTGGG GTTGGCAGGA CATTCACAGA 
CGCCTGCAAG ACTTCAGCCC TCAGCAGAAG AGCTCGCCAC GACCGCTCTA CTTCGTCAAG 
GTGGATGTGA GCGGAGCGTA TGACAGTCTC CCGCACCTGA AGCTGGTGGA GGTGCTGAAG 
GAAGTGTTGG GTCCGTTTGC AGAGCAGAGC TTCTTCCTGC GTCAGTACAG CAGTGTGTGG 
AGCGACCCGA CCCGCGGCCT GCGCAAACGC TTCTGCACCA AAGCTGAGAT GTCGGAGCCG 
CTCAACATGA AGGGGTTTGT TGTGGATGAA CAGGTCAGCG GGCGCCTGCA TGATGCTATA 
TTAGTGGAGC GGCACTCGTC TGAGGTCAGA GGTGGAGACG TCTTCCAGTT CTTCCAGAAG 
ATGCTCAGCA GTTACGTCAT CCATTACGAC CAGCAGATGT TCCGGCAGGT GTGTGGGATC 
CCGCAGGGCT CTTCTGTGTC TTCTCTGCTG TGTAATCTGT GTTACGGACA CATGGAGAAA 
GCCCTGCTGA AGGACATCGC TAAAGGAGGG TGTCTGATGA GGCTGATTGA TGATTTTTTG 
CTCATTACTC CTCATCTGAG TAAAGCCACA GAGTTTCTGA CCACTCTTCT GTCTGGAGTT 
CCAGATTACG GTTGCCAGAT TAACCCTCAG AAGGTGGCGG TGAACTTCCC GGTGTGTGTG 
TCCTGGGTAA ACTCGGGCGT CTCTGTGCTG CCGTCCAGCT GCCTGTTCCC CTGGTGCGGC 
TTGATGATAC ACACACACAC GCTGGACGTC TATAAAGACT ACTCACGGTA TTATATTTCT 
CCATTTACAC AATCTGACTG TCCAGACTGT ATGAATGCTC CGCCCACTCA GGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292199.1
CDS	564..3380
Translation	MSGQYSTDGGFRPVLEILRSLYPVVQTLEEFTDGLQFPDGRKPVLLEETDGARFKKLLSGLIVCAYTPPQLRVPAQLSTLPEVLAFTLNHIKRKKLRNVLGFGYQCSDVTTSSDPFRFHGDVSQTAASISTSEVWKRINQRLGTEVTRYLLQDCAVFTTVPPSCVLQVCGEPVYDLLMPRSWSGFFLSNSDNERISGAMRKSPAVQKTVAISKKRTRDNEKYISVKRRRVKETVNNNNGNYRSRCFAISKKRARDNEENISLKRRRMEETDQEAKIRNENHGSQSFTISKKRARDNEENISLKRQRMEEIDQVAKIRNENHGSQSWKPADQRPPRPSQCSIRVLSMLYNGRGMKNFLLNRKLKGVGGARRMQGEDLVRMIFLQSESNDSKPKKLPKRFFAMVPLFSRLLRQHRKCPYRLFLQRKCAGNPDVEDMESLLKSHSSPYRVYLFVRECLRHIIPHELWGCQENQLHFLSNVKNFLLLGKFERLTLVQLMWRMKVQACHWLGPKKRQCASEHRYREWMLGQCMGWVLSGFVVGLVRAQFYITESMGHKHTLRFYRGDVWSRLQDQAFRAHLCKGQWRPLSPSQALKVPNSAVTSRIRFIPKTSSMRPITRLSGSRDTLQYFQSCVRVLQNVLSVCVREAPGPMGSTVWGWQDIHRRLQDFSPQQKSSPRPLYFVKVDVSGAYDSLPHLKLVEVLKEVLGPFAEQSFFLRQYSSVWSDPTRGLRKRFCTKAEMSEPLNMKGFVVDEQVSGRLHDAILVERHSSEVRGGDVFQFFQKMLSSYVIHYDQQMFRQVCGIPQGSSVSSLLCNLCYGHMEKALLKDIAKGGCLMRLIDDFLLITPHLSKATEFLTTLLSGVPDYGCQINPQKVAVNFPVCVSWVNSGVSVLPSSCLFPWCGLMIHTHTLDVYKDYSRYYISPFTQSDCPDCMNAPPTQV

Target ORF information:

RefSeq Version	XM_009293924.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio telomerase reverse transcriptase (tert), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293924.2

ORF Insert Sequence:

ATGTCTGGAC AGTACTCGAC AGATGGCGGA TTCAGGCCGG TTTTGGAGAT TCTGCGCTCC 
TTATATCCAG TCGTGCAGAC TTTGGAGGAG TTCACCGACG GACTGCAATT CCCTGACGGC 
CGAAAGCCGG TTCTGCTGGA GGAAACAGAC GGCGCGCGCT TTAAAAAGCT CCTCAGTGGA 
CTTATTGTAT GCGCCTACAC GCCGCCGCAG CTGCGCGTCC CCGCCCAGCT CAGCACCCTG 
CCGGAGGTCT TGGCGTTCAC TCTGAACCAC ATTAAACGTA AGAAACTGAG GAACGTCCTG 
GGCTTCGGTT ATCAATGCAG CGACGTGACG ACCAGTTCGG ATCCCTTCCG TTTCCATGGT 
GACGTTTCGC AGACGGCTGC TTCCATCAGC ACCAGCGAGG TCTGGAAGCG TATCAACCAG 
CGTCTGGGCA CGGAGGTAAC GCGGTACCTG CTGCAGGACT GTGCCGTTTT CACCACCGTC 
CCGCCATCGT GTGTTCTGCA GGTGTGCGGA GAACCTGTTT ACGACTTGCT GATGCCGCGC 
TCATGGTCTG GCTTTTTCCT CAGTAACTCA GATAATGAAC GAATCAGCGG CGCGATGCGG 
AAATCCCCTG CTGTCCAGAA GACAGTCGCA ATTTCCAAAA AGAGAACAAG AGATAACGAA 
AAATATATTT CGGTAAAGCG GCGGAGGGTA AAGGAAACTG TGAATAATAA TAACGGAAAT 
TACAGATCTC GGTGTTTTGC AATTTCTAAA AAGAGAGCGA GAGATAATGA AGAAAATATT 
TCGTTAAAGC GACGGAGGAT GGAGGAAACT GACCAAGAAG CGAAAATACG TAATGAAAAT 
CACGGATCTC AGAGTTTCAC AATTTCTAAA AAGAGAGCGA GAGATAACGA AGAAAATATT 
TCGTTAAAGC GACAAAGGAT GGAGGAAATT GACCAAGTAG CGAAAATACG TAACGAAAAT 
CACGGATCTC AGAGTTGGAA ACCAGCGGAT CAGCGTCCTC CTCGACCCTC GCAATGTTCA 
ATACGCGTTC TGAGCATGCT CTACAATGGG CGGGGCATGA AGAACTTCCT GCTCAACAGG 
AAGTTGAAAG GAGTGGGCGG GGCCAGGCGC ATGCAAGGGG AGGATCTTGT CCGCATGATT 
TTCCTCCAAT CAGAATCCAA CGATAGCAAA CCGAAAAAAC TTCCTAAACG ATTCTTCGCA 
ATGGTGCCGC TATTCAGTCG GCTGTTGCGG CAGCACAGGA AGTGTCCGTA TCGGCTGTTC 
CTGCAAAGAA AGTGTGCAGG AAATCCAGAC GTGGAGGATA TGGAGTCTCT GCTGAAGTCA 
CACTCGTCTC CATATAGAGT TTATCTGTTC GTCAGGGAGT GTCTGCGCCA TATTATTCCC 
CACGAGCTCT GGGGCTGCCA GGAAAACCAG CTCCACTTCC TGTCTAATGT AAAGAACTTC 
CTGCTTCTGG GGAAGTTTGA GCGCCTCACG CTGGTCCAGC TGATGTGGAG GATGAAGGTT 
CAGGCCTGCC ATTGGCTGGG GCCCAAGAAA CGTCAGTGTG CGAGCGAGCA CCGCTACCGT 
GAGTGGATGT TGGGTCAGTG TATGGGCTGG GTGTTGAGTG GTTTTGTGGT CGGTCTGGTC 
AGAGCTCAGT TCTACATCAC GGAGAGTATG GGCCACAAAC ACACACTGCG CTTCTACAGG 
GGAGATGTCT GGAGCAGACT GCAGGACCAG GCCTTCAGGG CTCATCTGTG TAAGGGCCAG 
TGGAGGCCCC TGTCTCCATC CCAGGCGCTG AAGGTCCCCA ATAGTGCAGT GACATCCCGC 
ATCCGCTTTA TTCCCAAAAC CAGCAGCATG AGGCCCATCA CACGCCTCAG CGGCAGCAGA 
GACACACTGC AGTATTTTCA GAGCTGTGTG CGTGTGCTGC AGAATGTGTT GAGTGTGTGT 
GTGCGTGAGG CCCCGGGGCC CATGGGCTCC ACCGTCTGGG GTTGGCAGGA CATTCACAGA 
CGCCTGCAAG ACTTCAGCCC TCAGCAGAAG AGCTCGCCAC GACCGCTCTA CTTCGTCAAG 
GTGGATGTGA GCGGAGCGTA TGACAGTCTC CCGCACCTGA AGCTGGTGGA GGTGCTGAAG 
GAAGTGTTGG GTCCGTTTGC AGAGCAGAGC TTCTTCCTGC GTCAGTACAG CAGTGTGTGG 
AGCGACCCGA CCCGCGGCCT GCGCAAACGC TTCTGCACCA AAGCTGAGAT GTCGGAGCCG 
CTCAACATGA AGGGGTTTGT TGTGGATGAA CAGGTCAGCG GGCGCCTGCA TGATGCTATA 
TTAGTGGAGC GGCACTCGTC TGAGGTCAGA GGTGGAGACG TCTTCCAGTT CTTCCAGAAG 
ATGCTCAGCA GTTACGTCAT CCATTACGAC CAGCAGATGT TCCGGCAGGT GTGTGGGATC 
CCGCAGGGCT CTTCTGTGTC TTCTCTGCTG TGTAATCTGT GTTACGGACA CATGGAGAAA 
GCCCTGCTGA AGGACATCGC TAAAGGAGGG TGTCTGATGA GGCTGATTGA TGATTTTTTG 
CTCATTACTC CTCATCTGAG TAAAGCCACA GAGTTTCTGA CCACTCTTCT GTCTGGAGTT 
CCAGATTACG GTTGCCAGAT TAACCCTCAG AAGGTGGCGG TGAACTTCCC GGTGTGTGTG 
TCCTGGGTAA ACTCGGGCGT CTCTGTGCTG CCGTCCAGCT GCCTGTTCCC CTGGTGCGGC 
TTGATGATAC ACACACACAC GCTGGACGTC TATAAAGACT ACTCACGGTA TTATATTTCT 
CCATTTACAC AATCTGACTG TCCAGACTGT ATGAATGCTC CGCCCACTCA GGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Short Telomeres in Key Tissues Initiate Local and Systemic Aging in Zebrafish.
PLoS genetics12(1)e1005798(2016 Jan)
Carneiro MC,Henriques CM,Nabais J,Ferreira T,Carvalho T,Ferreira MG

DNA methylation of genes of the main components of the telomerase complex in Danio rerio.
Doklady. Biochemistry and biophysics464329-32(2015)
Belova EV,Kozlov AE,Shubernetskaya OS,Zvereva MI,Shpanchenko OV,Dontsova OA

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tert cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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