Him cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Him
Entrez Gene ID	32801
Full Name	Holes in muscle
Synonyms	CG15064,Dmel\CG15064,him
General protein information	Preferred Names Holes in muscle Names CG15064 gene product from transcript CG15064-RA CG15064-PA Him-PA holes in muscle holes in muscles holes-in-muscle
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 17A2-17A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_133045.4	NP_573273.1	holes in muscle

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Findings provide mechanistic insight into the brake on myogenesis that occurs during mesoderm specification: twist and tin expression at early stages in turn activate the myogenic inhibitor Him; yet, once Twist or Tin levels decline at mid-embryogenesis, Him is no longer expressed in the mesoderm, and MEF2-dependent muscle differentiation can proceed.
Title: The myogenic repressor gene Holes in muscles is a direct transcriptional target of Twist and Tinman in the Drosophila embryonic mesoderm.

Him may antagonise mef2 function during normal dorso-longitudinal muscle development.
Title: The Him gene inhibits the development of Drosophila flight muscles during metamorphosis.

Him expression rapidly declines as embryonic muscle differentiates, and consistent with this, Him overexpression inhibits muscle differentiation.
Title: The Him gene reveals a balance of inputs controlling muscle differentiation in Drosophila.

The following Him gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Him cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01990	NM_133045.4 Latest version!	Drosophila melanogaster holes in muscle (Him), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Him ( NM_133045.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_573273.1 Drosophila melanogaster holes in muscle (Him), mRNA.

Price & Availability↑

CloneID	OFa01990
Clone ID Related Accession (Same CDS sequence)	NM_133045.4
Accession Version	NM_133045.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	holes in muscle
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_133045.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCGTCA TCTACAAGAT ACTGAAGCAG CAACGCAGCA TGGATCTCAG CCTGAGCTCG 
GCCACCAGGT GCAAGGTGCA GGCGGCCATC AAGCAGCGCC AGCAGCAGCG CCGCCTTGAG 
GATCACATGG TGGAGGAGCG GGATCGGGAT CGGGAGCGGG ACCAGCAGCC GGATCAGGAT 
CAGAACCAGG TCCAGAATCA CATCGACAAG GATAAGGAGG AGCTGGCCGA GCAGCAATTA 
CAGCAGCTGT GCAGATTTCT AGCCGAGAAT GCGGCGCGAA AAAAGCGTCA GCGTTTCAAG 
CTGCAGTACC AATGCAATCT GGCCATCGAT CAGGATAACG ATCAGGAGCA GATGCCGGAG 
AAGGAGCACT TTGCAGCGCC GCCGCACGAG ATGGACCTCG AGTTCATAGA GCAGCTGCAG 
CAATCCTCGC CCGCCAAATC CCACGGAGCA ACTGGGCAGG CGCCACGTGA TAGCATCCTG 
CTGAAGATTC GCCACCAGAT CTTCGAGAGA AAGCGGCAGA GGCAGCGCCA GTTGGCGGAG 
CTGGTGCAGC AGCGAATGGT GGTGTGGCGA CCTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_573273.1
CDS	108..686
Translation	MGVIYKILKQQRSMDLSLSSATRCKVQAAIKQRQQQRRLEDHMVEERDRDRERDQQPDQDQNQVQNHIDKDKEELAEQQLQQLCRFLAENAARKKRQRFKLQYQCNLAIDQDNDQEQMPEKEHFAAPPHEMDLEFIEQLQQSSPAKSHGATGQAPRDSILLKIRHQIFERKRQRQRQLAELVQQRMVVWRPW

Target ORF information:

RefSeq Version	NM_133045.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster holes in muscle (Him), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133045.4

ORF Insert Sequence:

ATGGGCGTCA TCTACAAGAT ACTGAAGCAG CAACGCAGCA TGGATCTCAG CCTGAGCTCG 
GCCACCAGGT GCAAGGTGCA GGCGGCCATC AAGCAGCGCC AGCAGCAGCG CCGCCTTGAG 
GATCACATGG TGGAGGAGCG GGATCGGGAT CGGGAGCGGG ACCAGCAGCC GGATCAGGAT 
CAGAACCAGG TCCAGAATCA CATCGACAAG GATAAGGAGG AGCTGGCCGA GCAGCAATTA 
CAGCAGCTGT GCAGATTTCT AGCCGAGAAT GCGGCGCGAA AAAAGCGTCA GCGTTTCAAG 
CTGCAGTACC AATGCAATCT GGCCATCGAT CAGGATAACG ATCAGGAGCA GATGCCGGAG 
AAGGAGCACT TTGCAGCGCC GCCGCACGAG ATGGACCTCG AGTTCATAGA GCAGCTGCAG 
CAATCCTCGC CCGCCAAATC CCACGGAGCA ACTGGGCAGG CGCCACGTGA TAGCATCCTG 
CTGAAGATTC GCCACCAGAT CTTCGAGAGA AAGCGGCAGA GGCAGCGCCA GTTGGCGGAG 
CTGGTGCAGC AGCGAATGGT GGTGTGGCGA CCTTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Him cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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