Sps2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sps2
Entrez Gene ID	34397
Full Name	Selenophosphate synthetase 2
Synonyms	CG5025,Dmel\CG5025,Dsps2,SPS2,dSPS2,dsps2,sps2
General protein information	Preferred Names Selenophosphate synthetase 2 Names CG5025 gene product from transcript CG5025-RA CG5025-PA CG5025-PC Sps2-PA Sps2-PC selenophosphate synthetase selenophosphate synthetase 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 31D9-31D9

mRNA and Protein(s)

mRNA	Protein	Name
NM_058130.6	NP_477478.4	selenophosphate synthetase 2, isoform A
NM_001273400.2	NP_001260329.1	selenophosphate synthetase 2, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the transcriptome of fruit flies with knockdown of SPS2 in larval and adult stages, is reported.[Sps2]
Title: Gene expression profiling of selenophosphate synthetase 2 knockdown in Drosophila melanogaster.

The downstream DNA replication-related element has been identified as an essential element for dsps2 promoter activity.
Title: A DNA replication-related element downstream from the initiation site of Drosophila selenophosphate synthetase 2 gene is essential for its transcription.

The following Sps2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sps2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14328	NM_001273400.2 Latest version!	Drosophila melanogaster selenophosphate synthetase 2, transcript variant C (Sps2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa14328	NM_058130.6 Latest version!	Drosophila melanogaster selenophosphate synthetase 2, transcript variant A (Sps2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sps2 ( NM_058130.6 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477478.4 Drosophila melanogaster selenophosphate synthetase 2, transcript variant A (Sps2), mRNA.

Price & Availability↑

CloneID	OFa14328
Clone ID Related Accession (Same CDS sequence)	NM_058130.6 , NM_001273400.2
Accession Version	NM_058130.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	selenophosphate synthetase 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_058130.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTCAAC CCGAAAAACA TGGGCTTGAG CCCGACTTTC AGCTTACTAA GTTTACCACC 
CACACCGGGT GAAGTTGCAA AATCCCCCAA AAAGTACTGG AGAAGTACCT AAGGGGAACA 
GAAATAGAAA ATAAGAATAA TGATGGATAC CTTATCGGCT CTGGAATGGA CTGTGCAGTT 
ATACCACTCA AGCGGCACAA GGACTATTTG CTTATTCAAA CCGTTGACTT CTTCTATCCC 
ATGGTTAATG ATCCCGAATT ACTGGGTCGT ATTGCGCTAG CTAATGTCTT GAGTGATGTC 
TATGCGGTTG GGGTGACGCA GTTTGATACA GTGGAGATGA TCGTCAGCAC TTCCACAAGT 
TTCAGTGAGA AGGAACGCGA CGTTGTGATT GGATTGGTGA TGAAGGGTTT CCAAAACTCC 
CTGAAGGCAA ATGGCTATCG TAATACCCCG CTGATTATTA GGCAATTGAA GATCAATCCG 
TGGTGCATTA TTGGTGGCAT AGCCACATCT GTCTGCCGTA GCGAGGAAAT AATACTACCG 
TCAAACGCGC AGCCCGGCGA TGTATTGGTC CTCACGAAAC CTTTGGGCGG TCAAATGGCG 
ATGGATGCTC ACCTTTGGCA GCTTAACCAG ACCGAAAAGT ACAAGAAACT GCTGTCTGAA 
TGTAGTGATG CTGATATAAA AGAAACCTTT GAAATAGCAG TGAAATCTAT GACCTATTTG 
AACAAAAATG CCGCCCTCTT AATGCACAAA TATCAAGCGC ACTGTGCCAC TGATATCACC 
GGATTTGGTT TACTGGGTCA CGCCAATAAT CTAGCACAAT TCCAAAAGGA AAAGGTTTTA 
TTCCAAATCA ACAAGTTGCC CATCATCAAA AATGTTCTTA AATTCAGCAC CTTGGTGGGC 
CAAAGCACAA AGTTTCGTTC TGGCAGATCG GTGGAAACCT CTGGAGGCCT TTTAATATGC 
CTTCCTGCTG ATGCCGCTGA TAAATTTTGC CGCGACTTTG AAGAAGCTAC CAACGGAGAG 
CAGAAATCCT TTCAAATTGG ACATGTTACA GCCGCAAATG AGTCTGATGC AGTTCTGTGC 
GAAGATGTAG AGTTTATCGA AGTTAGCCTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477478.4
CDS	166..1278
Translation	MFQPEKHGLEPDFQLTKFTTHTG*SCKIPQKVLEKYLRGTEIENKNNDGYLIGSGMDCAVIPLKRHKDYLLIQTVDFFYPMVNDPELLGRIALANVLSDVYAVGVTQFDTVEMIVSTSTSFSEKERDVVIGLVMKGFQNSLKANGYRNTPLIIRQLKINPWCIIGGIATSVCRSEEIILPSNAQPGDVLVLTKPLGGQMAMDAHLWQLNQTEKYKKLLSECSDADIKETFEIAVKSMTYLNKNAALLMHKYQAHCATDITGFGLLGHANNLAQFQKEKVLFQINKLPIIKNVLKFSTLVGQSTKFRSGRSVETSGGLLICLPADAADKFCRDFEEATNGEQKSFQIGHVTAANESDAVLCEDVEFIEVSL

Target ORF information:

RefSeq Version	NM_058130.6
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster selenophosphate synthetase 2, transcript variant A (Sps2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058130.6

ORF Insert Sequence:

ATGTTTCAAC CCGAAAAACA TGGGCTTGAG CCCGACTTTC AGCTTACTAA GTTTACCACC 
CACACCGGGT GAAGTTGCAA AATCCCCCAA AAAGTACTGG AGAAGTACCT AAGGGGAACA 
GAAATAGAAA ATAAGAATAA TGATGGATAC CTTATCGGCT CTGGAATGGA CTGTGCAGTT 
ATACCACTCA AGCGGCACAA GGACTATTTG CTTATTCAAA CCGTTGACTT CTTCTATCCC 
ATGGTTAATG ATCCCGAATT ACTGGGTCGT ATTGCGCTAG CTAATGTCTT GAGTGATGTC 
TATGCGGTTG GGGTGACGCA GTTTGATACA GTGGAGATGA TCGTCAGCAC TTCCACAAGT 
TTCAGTGAGA AGGAACGCGA CGTTGTGATT GGATTGGTGA TGAAGGGTTT CCAAAACTCC 
CTGAAGGCAA ATGGCTATCG TAATACCCCG CTGATTATTA GGCAATTGAA GATCAATCCG 
TGGTGCATTA TTGGTGGCAT AGCCACATCT GTCTGCCGTA GCGAGGAAAT AATACTACCG 
TCAAACGCGC AGCCCGGCGA TGTATTGGTC CTCACGAAAC CTTTGGGCGG TCAAATGGCG 
ATGGATGCTC ACCTTTGGCA GCTTAACCAG ACCGAAAAGT ACAAGAAACT GCTGTCTGAA 
TGTAGTGATG CTGATATAAA AGAAACCTTT GAAATAGCAG TGAAATCTAT GACCTATTTG 
AACAAAAATG CCGCCCTCTT AATGCACAAA TATCAAGCGC ACTGTGCCAC TGATATCACC 
GGATTTGGTT TACTGGGTCA CGCCAATAAT CTAGCACAAT TCCAAAAGGA AAAGGTTTTA 
TTCCAAATCA ACAAGTTGCC CATCATCAAA AATGTTCTTA AATTCAGCAC CTTGGTGGGC 
CAAAGCACAA AGTTTCGTTC TGGCAGATCG GTGGAAACCT CTGGAGGCCT TTTAATATGC 
CTTCCTGCTG ATGCCGCTGA TAAATTTTGC CGCGACTTTG AAGAAGCTAC CAACGGAGAG 
CAGAAATCCT TTCAAATTGG ACATGTTACA GCCGCAAATG AGTCTGATGC AGTTCTGTGC 
GAAGATGTAG AGTTTATCGA AGTTAGCCTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sps2 ( NM_001273400.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260329.1 Drosophila melanogaster selenophosphate synthetase 2, transcript variant C (Sps2), mRNA.

Price & Availability↑

CloneID	OFa14328
Clone ID Related Accession (Same CDS sequence)	NM_058130.6 , NM_001273400.2
Accession Version	NM_001273400.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	selenophosphate synthetase 2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001273400.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTCAAC CCGAAAAACA TGGGCTTGAG CCCGACTTTC AGCTTACTAA GTTTACCACC 
CACACCGGGT GAAGTTGCAA AATCCCCCAA AAAGTACTGG AGAAGTACCT AAGGGGAACA 
GAAATAGAAA ATAAGAATAA TGATGGATAC CTTATCGGCT CTGGAATGGA CTGTGCAGTT 
ATACCACTCA AGCGGCACAA GGACTATTTG CTTATTCAAA CCGTTGACTT CTTCTATCCC 
ATGGTTAATG ATCCCGAATT ACTGGGTCGT ATTGCGCTAG CTAATGTCTT GAGTGATGTC 
TATGCGGTTG GGGTGACGCA GTTTGATACA GTGGAGATGA TCGTCAGCAC TTCCACAAGT 
TTCAGTGAGA AGGAACGCGA CGTTGTGATT GGATTGGTGA TGAAGGGTTT CCAAAACTCC 
CTGAAGGCAA ATGGCTATCG TAATACCCCG CTGATTATTA GGCAATTGAA GATCAATCCG 
TGGTGCATTA TTGGTGGCAT AGCCACATCT GTCTGCCGTA GCGAGGAAAT AATACTACCG 
TCAAACGCGC AGCCCGGCGA TGTATTGGTC CTCACGAAAC CTTTGGGCGG TCAAATGGCG 
ATGGATGCTC ACCTTTGGCA GCTTAACCAG ACCGAAAAGT ACAAGAAACT GCTGTCTGAA 
TGTAGTGATG CTGATATAAA AGAAACCTTT GAAATAGCAG TGAAATCTAT GACCTATTTG 
AACAAAAATG CCGCCCTCTT AATGCACAAA TATCAAGCGC ACTGTGCCAC TGATATCACC 
GGATTTGGTT TACTGGGTCA CGCCAATAAT CTAGCACAAT TCCAAAAGGA AAAGGTTTTA 
TTCCAAATCA ACAAGTTGCC CATCATCAAA AATGTTCTTA AATTCAGCAC CTTGGTGGGC 
CAAAGCACAA AGTTTCGTTC TGGCAGATCG GTGGAAACCT CTGGAGGCCT TTTAATATGC 
CTTCCTGCTG ATGCCGCTGA TAAATTTTGC CGCGACTTTG AAGAAGCTAC CAACGGAGAG 
CAGAAATCCT TTCAAATTGG ACATGTTACA GCCGCAAATG AGTCTGATGC AGTTCTGTGC 
GAAGATGTAG AGTTTATCGA AGTTAGCCTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260329.1
CDS	58..1170
Translation	MFQPEKHGLEPDFQLTKFTTHTG*SCKIPQKVLEKYLRGTEIENKNNDGYLIGSGMDCAVIPLKRHKDYLLIQTVDFFYPMVNDPELLGRIALANVLSDVYAVGVTQFDTVEMIVSTSTSFSEKERDVVIGLVMKGFQNSLKANGYRNTPLIIRQLKINPWCIIGGIATSVCRSEEIILPSNAQPGDVLVLTKPLGGQMAMDAHLWQLNQTEKYKKLLSECSDADIKETFEIAVKSMTYLNKNAALLMHKYQAHCATDITGFGLLGHANNLAQFQKEKVLFQINKLPIIKNVLKFSTLVGQSTKFRSGRSVETSGGLLICLPADAADKFCRDFEEATNGEQKSFQIGHVTAANESDAVLCEDVEFIEVSL

Target ORF information:

RefSeq Version	NM_001273400.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster selenophosphate synthetase 2, transcript variant C (Sps2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273400.2

ORF Insert Sequence:

ATGTTTCAAC CCGAAAAACA TGGGCTTGAG CCCGACTTTC AGCTTACTAA GTTTACCACC 
CACACCGGGT GAAGTTGCAA AATCCCCCAA AAAGTACTGG AGAAGTACCT AAGGGGAACA 
GAAATAGAAA ATAAGAATAA TGATGGATAC CTTATCGGCT CTGGAATGGA CTGTGCAGTT 
ATACCACTCA AGCGGCACAA GGACTATTTG CTTATTCAAA CCGTTGACTT CTTCTATCCC 
ATGGTTAATG ATCCCGAATT ACTGGGTCGT ATTGCGCTAG CTAATGTCTT GAGTGATGTC 
TATGCGGTTG GGGTGACGCA GTTTGATACA GTGGAGATGA TCGTCAGCAC TTCCACAAGT 
TTCAGTGAGA AGGAACGCGA CGTTGTGATT GGATTGGTGA TGAAGGGTTT CCAAAACTCC 
CTGAAGGCAA ATGGCTATCG TAATACCCCG CTGATTATTA GGCAATTGAA GATCAATCCG 
TGGTGCATTA TTGGTGGCAT AGCCACATCT GTCTGCCGTA GCGAGGAAAT AATACTACCG 
TCAAACGCGC AGCCCGGCGA TGTATTGGTC CTCACGAAAC CTTTGGGCGG TCAAATGGCG 
ATGGATGCTC ACCTTTGGCA GCTTAACCAG ACCGAAAAGT ACAAGAAACT GCTGTCTGAA 
TGTAGTGATG CTGATATAAA AGAAACCTTT GAAATAGCAG TGAAATCTAT GACCTATTTG 
AACAAAAATG CCGCCCTCTT AATGCACAAA TATCAAGCGC ACTGTGCCAC TGATATCACC 
GGATTTGGTT TACTGGGTCA CGCCAATAAT CTAGCACAAT TCCAAAAGGA AAAGGTTTTA 
TTCCAAATCA ACAAGTTGCC CATCATCAAA AATGTTCTTA AATTCAGCAC CTTGGTGGGC 
CAAAGCACAA AGTTTCGTTC TGGCAGATCG GTGGAAACCT CTGGAGGCCT TTTAATATGC 
CTTCCTGCTG ATGCCGCTGA TAAATTTTGC CGCGACTTTG AAGAAGCTAC CAACGGAGAG 
CAGAAATCCT TTCAAATTGG ACATGTTACA GCCGCAAATG AGTCTGATGC AGTTCTGTGC 
GAAGATGTAG AGTTTATCGA AGTTAGCCTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sps2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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