Letm1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Letm1
Entrez Gene ID	37912
Full Name	LETM1 ortholog
Synonyms	B4D,CG4589,DmLETM1,Dmel\CG4589,anon-60Da,anon60Da,dLetm1,l(2)k10502,letm1
General protein information	Preferred Names LETM1 ortholog Names CG4589 gene product from transcript CG4589-RB CG4589-PA CG4589-PB CG4589-PC Letm1-PA Letm1-PB Letm1-PC lethal (2) k10502 leucine zipper and EF-hand containing transmembrane protein 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60D3-60D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_166675.2	NP_726454.1	leucine zipper and EF-hand containing transmembrane protein 1, isoform C
NM_138078.3	NP_611922.1	leucine zipper and EF-hand containing transmembrane protein 1, isoform B
NM_166674.2	NP_726453.1	leucine zipper and EF-hand containing transmembrane protein 1, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results demonstrate the function of DmLETM1 as a mitochondrial osmoregulator.
Title: A Drosophila mutant of LETM1, a candidate gene for seizures in Wolf-Hirschhorn syndrome.

CG4589, the Drosophila homolog of the human gene Letm1, was identified as a gene strongly affecting [Ca2+]mito and [H+]mito responses
Title: Genome-wide RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+ antiporter.

The following Letm1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Letm1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11183	NM_138078.3 Latest version!	Drosophila melanogaster leucine zipper and EF-hand containing transmembrane protein 1, transcript variant B (Letm1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa11183	NM_166675.2 Latest version!	Drosophila melanogaster leucine zipper and EF-hand containing transmembrane protein 1, transcript variant C (Letm1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa11183	NM_166674.2 Latest version!	Drosophila melanogaster leucine zipper and EF-hand containing transmembrane protein 1, transcript variant A (Letm1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Letm1 ( NM_166675.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726454.1 Drosophila melanogaster leucine zipper and EF-hand containing transmembrane protein 1, transcript variant C (Letm1), mRNA.

Price & Availability↑

CloneID	OFa11183
Clone ID Related Accession (Same CDS sequence)	NM_166675.2 , NM_138078.3 , NM_166674.2
Accession Version	NM_166675.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	leucine zipper and EF-hand containing transmembrane protein 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166675.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACGCCC TGCTGCGTCA CAAGGGACGC AATCTGCGGA CGAGTCACTT GGCCCAGAAT 
GTCTACAAGC GCTTTCTTAA ATCGAACTGT TGCGCATGCA GTTCGGTGAA CGTCACAGAT 
GAACCCGCCA AAGAAGATGA ACTACCGCGA AGATCTGCTT CGACCTCGGT GCTGGAGCTA 
AGCAGATCGC TTGGCACTTA CCGAAGGTTT CAGCCACATG CCAACTATGG ATACGACTAC 
TCCGGCTATG GATTTAGGCA TCTGCATACG AGCCGGACTT TGCTGGAGAC GTCCAGCTCA 
AAGATCGACG CAACAGTCAA GAAGCTGAAA AACCAGCAGA AGGAGAAGGT GGAGGAGATC 
ATGAAGGAGG TGGCCAATGG ACAGGCCGCG GCAGTTCGTG CCAGCAGTGC CGCTACCGCC 
ACGGCCTCCT CGGAGAAGGG TCAAAACGCT AGTGCGACCG CGGGCTCCAC GTCGGCGACT 
GCCTCAACGA CGTCTTTGGC CAAGACCGCA GACAAGTCGG TGGCCAAGCC AAAGAAACCA 
CTCCGCACAC GGATTTGGGA TGAGCTGGTG CACTATTACC ACGGCTTTAG GCTGCTCTTC 
ATCGATGTGG CAATTTGCTC TAAGCTCCTT TGGCGAGTGC TCAACGGCAA GACGCTCACG 
CGGCGCGAGA ACAAGCAGCT TCAGCGGACC ACCTCTGACT TGTTCCGTCT GATCCCCTTT 
TCGGTGTTCA TCATCGTGCC CTTCATGGAG CTGCTGCTGC CGTTGTTCAT AAAATTCTTC 
CCCGGCATGC TGCCCTCCAC GTTCCAGACT TCGACCGATC GACAGGAGAA GCTCCGGCAG 
TCACTTAGCG TTCGCCTGGA GGTGGCCAAG TTCTTGCAGC AGACCCTAGA TCAGATGCCC 
GTCCAGCACA AGGAACACAG CAGCGAAGAG GCCAAGCAGT TCGAGGCCTT TTTCACTAAA 
ATCCGGAATC CCACTGAACC AGTGAGCAAC GATGAAATCA TCAAATTCGC CAAGCGATTC 
GACGATGAGA TCACCTTAGA TTCACTGTCC CGGGAGCAAT TGGCGGCTCT TTGTCGCGTC 
CTTGAACTGA ACACAATTGG CACCACCACG CTGCTGCGGT TCCAGCTGCG TCTGAAGCTG 
AGATCTTTGG CCACCGATGA CCGCGTTATT GCCCGCGAAG GAGTCGACTC CCTGGACCTT 
CTAGAGCTGC AACAGGCGTG CAAGGCACGT GGAATGCGGG CATATGGCCT AACGGAGGAG 
CGTCTTCGTT TTCAGCTTAA GGAGTGGATA GATCTCTCCC TGAACGAACA GGTACCGCCT 
ACGCTGCTGC TCCTGTCAAG AACCATGCTT ATTTCCGACG ACAGCATCAC CACCGATAAG 
CTCAAGGAGA CGATTCGTGT GCTACCGGAT GCAGTGGGTG CCCACACACG GCACGCTATC 
GGGGAGAGTG AGGGCAAGGT GGACAACAAG ACGAAGATCG AAATCATCAA GGAGGAGGAG 
CGCAAGATTC GCGAAGAGCG CGAGGAAGAG CGGGAAGAGA CCATCGCAAA ACGATCGGCC 
ATCAAGGAGG AGATTCCCGC TCCTTATGTA TTTGCTGAGA AGTTGTCGGG TTCCCAGGAT 
CTTCTGGATC ACAAGGAACA GTCCTCGGTG TCCGAGACGG ACAAGGGCAT TTCCTCTACC 
GATGTACAGC TGCTGTCTGA GGCCCTAAAG ACACTCTCAT CTGATAAACA GCTCGTGGTG 
GAAAAGGAAA CAATCAAGGA ACTCAAGGAG GAATTGGCCG ACTATAAGGA GGATGTGGAG 
GAGCTACGCG AGGTGCGACA GGTAGTCAAG GAGCCTGTGC GCGAAAGCCG GGCGGCCAAG 
TTGCTCTACA ATCGGGTCAA CAAGATGATT TCCCAACTCG ATAATGTGCT TAACGACCTA 
GAGGCCAGGC AGCATCAAAT CAAGCAGGCG GAATCCTCTG ATTACGCGGC GTCCAGTCCC 
ACTGTGGAAC CACAGCAAAT GGTGCACATT GACGAGCTGG TCGCCACCAT CCGGCGAATG 
AAGGAGGCAT CCGATGAAGA GCGATTCAAG GTTGTCGGGG ATCTGCTGGT CAAACTCGAC 
GCGGACAAAG ACGGTGTGAT TTCGGTGAAC GAGATCACCA AGGCGGTGCA GAGCATCGAC 
CGCGAGGCCA CGAATATCGA CAAGAAGCAG CTGGAGGAGT TTACCGAGCT ACTTTCCAAG 
TTGGCGTCCC GGCGACGACA CGAGGAGATC GTGCACATCG ACGATTTAAT GAATAACATC 
AAGGTGTTGA AGGAAACTAG CGATGAGGCA CGACTTAAGC ACATCGAAGC TGTACTGGAG 
AAATTTGATG CCGACAAGGA TGGCGTGGTG ACCGTCAACG ATATTCGTAA GGTGTTGGAA 
TCCATTGGTC GTGATAACAT CAAGCTGAGC GACAAGGCCA TTGAGGAACT CATCAGTCTG 
CTTGACAAGG AGCAAGTACT GCAGGCAGAG CAGAAGATTG AGAAGGCCAT TGCTAAGAGC 
ATGAAAGAGG CTGAGAAACT GAAGTCTGAG GTGGACAAAG CGGATAAGGA CTTGTCCAAG 
CTTGTTAATG ATATTCACGA TTCCGCCAAG GAGATTCAGG ATATTGCCAA CGAGATGCGT 
GATAAGGAGG AAACTGTGCC CGATAAAGCG AAGGAGCTAA AAGCAGAGCC CGCATTCAAG 
GATACGGCCA AAACATTGAA GGACAACGCA AAGGACCTGG ACGATTTAGC GAAGGACCCA 
AAGTCAGACC CCAAATCCCC GACAAAGGCT TCTACAGGGT CCGGTCCAGC CGGACTGTCA 
GGTGGAGGCC CCAGCAGTGG CAGTAGCGGT ATTGCCACCG GATCAACGAC GGAATCAGCG 
CTCCGAGAGG CGGCCGAGCG TCAAATGGAA AAGATCCTGC CCTCCACAGA CATTGGTCTG 
CCCCCCACGA TACAAACACC ATCGCAACCA CCGACGTCAA AAAAAGCAAC GGCCACTGCG 
TCGACCCTGT CCACGACGAT AACGGCCAAG AAACTGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726454.1
CDS	390..3431
Translation	MNALLRHKGRNLRTSHLAQNVYKRFLKSNCCACSSVNVTDEPAKEDELPRRSASTSVLELSRSLGTYRRFQPHANYGYDYSGYGFRHLHTSRTLLETSSSKIDATVKKLKNQQKEKVEEIMKEVANGQAAAVRASSAATATASSEKGQNASATAGSTSATASTTSLAKTADKSVAKPKKPLRTRIWDELVHYYHGFRLLFIDVAICSKLLWRVLNGKTLTRRENKQLQRTTSDLFRLIPFSVFIIVPFMELLLPLFIKFFPGMLPSTFQTSTDRQEKLRQSLSVRLEVAKFLQQTLDQMPVQHKEHSSEEAKQFEAFFTKIRNPTEPVSNDEIIKFAKRFDDEITLDSLSREQLAALCRVLELNTIGTTTLLRFQLRLKLRSLATDDRVIAREGVDSLDLLELQQACKARGMRAYGLTEERLRFQLKEWIDLSLNEQVPPTLLLLSRTMLISDDSITTDKLKETIRVLPDAVGAHTRHAIGESEGKVDNKTKIEIIKEEERKIREEREEEREETIAKRSAIKEEIPAPYVFAEKLSGSQDLLDHKEQSSVSETDKGISSTDVQLLSEALKTLSSDKQLVVEKETIKELKEELADYKEDVEELREVRQVVKEPVRESRAAKLLYNRVNKMISQLDNVLNDLEARQHQIKQAESSDYAASSPTVEPQQMVHIDELVATIRRMKEASDEERFKVVGDLLVKLDADKDGVISVNEITKAVQSIDREATNIDKKQLEEFTELLSKLASRRRHEEIVHIDDLMNNIKVLKETSDEARLKHIEAVLEKFDADKDGVVTVNDIRKVLESIGRDNIKLSDKAIEELISLLDKEQVLQAEQKIEKAIAKSMKEAEKLKSEVDKADKDLSKLVNDIHDSAKEIQDIANEMRDKEETVPDKAKELKAEPAFKDTAKTLKDNAKDLDDLAKDPKSDPKSPTKASTGSGPAGLSGGGPSSGSSGIATGSTTESALREAAERQMEKILPSTDIGLPPTIQTPSQPPTSKKATATASTLSTTITAKKLL

Target ORF information:

RefSeq Version	NM_166675.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster leucine zipper and EF-hand containing transmembrane protein 1, transcript variant C (Letm1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166675.2

ORF Insert Sequence:

ATGAACGCCC TGCTGCGTCA CAAGGGACGC AATCTGCGGA CGAGTCACTT GGCCCAGAAT 
GTCTACAAGC GCTTTCTTAA ATCGAACTGT TGCGCATGCA GTTCGGTGAA CGTCACAGAT 
GAACCCGCCA AAGAAGATGA ACTACCGCGA AGATCTGCTT CGACCTCGGT GCTGGAGCTA 
AGCAGATCGC TTGGCACTTA CCGAAGGTTT CAGCCACATG CCAACTATGG ATACGACTAC 
TCCGGCTATG GATTTAGGCA TCTGCATACG AGCCGGACTT TGCTGGAGAC GTCCAGCTCA 
AAGATCGACG CAACAGTCAA GAAGCTGAAA AACCAGCAGA AGGAGAAGGT GGAGGAGATC 
ATGAAGGAGG TGGCCAATGG ACAGGCCGCG GCAGTTCGTG CCAGCAGTGC CGCTACCGCC 
ACGGCCTCCT CGGAGAAGGG TCAAAACGCT AGTGCGACCG CGGGCTCCAC GTCGGCGACT 
GCCTCAACGA CGTCTTTGGC CAAGACCGCA GACAAGTCGG TGGCCAAGCC AAAGAAACCA 
CTCCGCACAC GGATTTGGGA TGAGCTGGTG CACTATTACC ACGGCTTTAG GCTGCTCTTC 
ATCGATGTGG CAATTTGCTC TAAGCTCCTT TGGCGAGTGC TCAACGGCAA GACGCTCACG 
CGGCGCGAGA ACAAGCAGCT TCAGCGGACC ACCTCTGACT TGTTCCGTCT GATCCCCTTT 
TCGGTGTTCA TCATCGTGCC CTTCATGGAG CTGCTGCTGC CGTTGTTCAT AAAATTCTTC 
CCCGGCATGC TGCCCTCCAC GTTCCAGACT TCGACCGATC GACAGGAGAA GCTCCGGCAG 
TCACTTAGCG TTCGCCTGGA GGTGGCCAAG TTCTTGCAGC AGACCCTAGA TCAGATGCCC 
GTCCAGCACA AGGAACACAG CAGCGAAGAG GCCAAGCAGT TCGAGGCCTT TTTCACTAAA 
ATCCGGAATC CCACTGAACC AGTGAGCAAC GATGAAATCA TCAAATTCGC CAAGCGATTC 
GACGATGAGA TCACCTTAGA TTCACTGTCC CGGGAGCAAT TGGCGGCTCT TTGTCGCGTC 
CTTGAACTGA ACACAATTGG CACCACCACG CTGCTGCGGT TCCAGCTGCG TCTGAAGCTG 
AGATCTTTGG CCACCGATGA CCGCGTTATT GCCCGCGAAG GAGTCGACTC CCTGGACCTT 
CTAGAGCTGC AACAGGCGTG CAAGGCACGT GGAATGCGGG CATATGGCCT AACGGAGGAG 
CGTCTTCGTT TTCAGCTTAA GGAGTGGATA GATCTCTCCC TGAACGAACA GGTACCGCCT 
ACGCTGCTGC TCCTGTCAAG AACCATGCTT ATTTCCGACG ACAGCATCAC CACCGATAAG 
CTCAAGGAGA CGATTCGTGT GCTACCGGAT GCAGTGGGTG CCCACACACG GCACGCTATC 
GGGGAGAGTG AGGGCAAGGT GGACAACAAG ACGAAGATCG AAATCATCAA GGAGGAGGAG 
CGCAAGATTC GCGAAGAGCG CGAGGAAGAG CGGGAAGAGA CCATCGCAAA ACGATCGGCC 
ATCAAGGAGG AGATTCCCGC TCCTTATGTA TTTGCTGAGA AGTTGTCGGG TTCCCAGGAT 
CTTCTGGATC ACAAGGAACA GTCCTCGGTG TCCGAGACGG ACAAGGGCAT TTCCTCTACC 
GATGTACAGC TGCTGTCTGA GGCCCTAAAG ACACTCTCAT CTGATAAACA GCTCGTGGTG 
GAAAAGGAAA CAATCAAGGA ACTCAAGGAG GAATTGGCCG ACTATAAGGA GGATGTGGAG 
GAGCTACGCG AGGTGCGACA GGTAGTCAAG GAGCCTGTGC GCGAAAGCCG GGCGGCCAAG 
TTGCTCTACA ATCGGGTCAA CAAGATGATT TCCCAACTCG ATAATGTGCT TAACGACCTA 
GAGGCCAGGC AGCATCAAAT CAAGCAGGCG GAATCCTCTG ATTACGCGGC GTCCAGTCCC 
ACTGTGGAAC CACAGCAAAT GGTGCACATT GACGAGCTGG TCGCCACCAT CCGGCGAATG 
AAGGAGGCAT CCGATGAAGA GCGATTCAAG GTTGTCGGGG ATCTGCTGGT CAAACTCGAC 
GCGGACAAAG ACGGTGTGAT TTCGGTGAAC GAGATCACCA AGGCGGTGCA GAGCATCGAC 
CGCGAGGCCA CGAATATCGA CAAGAAGCAG CTGGAGGAGT TTACCGAGCT ACTTTCCAAG 
TTGGCGTCCC GGCGACGACA CGAGGAGATC GTGCACATCG ACGATTTAAT GAATAACATC 
AAGGTGTTGA AGGAAACTAG CGATGAGGCA CGACTTAAGC ACATCGAAGC TGTACTGGAG 
AAATTTGATG CCGACAAGGA TGGCGTGGTG ACCGTCAACG ATATTCGTAA GGTGTTGGAA 
TCCATTGGTC GTGATAACAT CAAGCTGAGC GACAAGGCCA TTGAGGAACT CATCAGTCTG 
CTTGACAAGG AGCAAGTACT GCAGGCAGAG CAGAAGATTG AGAAGGCCAT TGCTAAGAGC 
ATGAAAGAGG CTGAGAAACT GAAGTCTGAG GTGGACAAAG CGGATAAGGA CTTGTCCAAG 
CTTGTTAATG ATATTCACGA TTCCGCCAAG GAGATTCAGG ATATTGCCAA CGAGATGCGT 
GATAAGGAGG AAACTGTGCC CGATAAAGCG AAGGAGCTAA AAGCAGAGCC CGCATTCAAG 
GATACGGCCA AAACATTGAA GGACAACGCA AAGGACCTGG ACGATTTAGC GAAGGACCCA 
AAGTCAGACC CCAAATCCCC GACAAAGGCT TCTACAGGGT CCGGTCCAGC CGGACTGTCA 
GGTGGAGGCC CCAGCAGTGG CAGTAGCGGT ATTGCCACCG GATCAACGAC GGAATCAGCG 
CTCCGAGAGG CGGCCGAGCG TCAAATGGAA AAGATCCTGC CCTCCACAGA CATTGGTCTG 
CCCCCCACGA TACAAACACC ATCGCAACCA CCGACGTCAA AAAAAGCAAC GGCCACTGCG 
TCGACCCTGT CCACGACGAT AACGGCCAAG AAACTGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Letm1 ( NM_138078.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611922.1 Drosophila melanogaster leucine zipper and EF-hand containing transmembrane protein 1, transcript variant B (Letm1), mRNA.

Price & Availability↑

CloneID	OFa11183
Clone ID Related Accession (Same CDS sequence)	NM_166675.2 , NM_138078.3 , NM_166674.2
Accession Version	NM_138078.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	leucine zipper and EF-hand containing transmembrane protein 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_138078.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACGCCC TGCTGCGTCA CAAGGGACGC AATCTGCGGA CGAGTCACTT GGCCCAGAAT 
GTCTACAAGC GCTTTCTTAA ATCGAACTGT TGCGCATGCA GTTCGGTGAA CGTCACAGAT 
GAACCCGCCA AAGAAGATGA ACTACCGCGA AGATCTGCTT CGACCTCGGT GCTGGAGCTA 
AGCAGATCGC TTGGCACTTA CCGAAGGTTT CAGCCACATG CCAACTATGG ATACGACTAC 
TCCGGCTATG GATTTAGGCA TCTGCATACG AGCCGGACTT TGCTGGAGAC GTCCAGCTCA 
AAGATCGACG CAACAGTCAA GAAGCTGAAA AACCAGCAGA AGGAGAAGGT GGAGGAGATC 
ATGAAGGAGG TGGCCAATGG ACAGGCCGCG GCAGTTCGTG CCAGCAGTGC CGCTACCGCC 
ACGGCCTCCT CGGAGAAGGG TCAAAACGCT AGTGCGACCG CGGGCTCCAC GTCGGCGACT 
GCCTCAACGA CGTCTTTGGC CAAGACCGCA GACAAGTCGG TGGCCAAGCC AAAGAAACCA 
CTCCGCACAC GGATTTGGGA TGAGCTGGTG CACTATTACC ACGGCTTTAG GCTGCTCTTC 
ATCGATGTGG CAATTTGCTC TAAGCTCCTT TGGCGAGTGC TCAACGGCAA GACGCTCACG 
CGGCGCGAGA ACAAGCAGCT TCAGCGGACC ACCTCTGACT TGTTCCGTCT GATCCCCTTT 
TCGGTGTTCA TCATCGTGCC CTTCATGGAG CTGCTGCTGC CGTTGTTCAT AAAATTCTTC 
CCCGGCATGC TGCCCTCCAC GTTCCAGACT TCGACCGATC GACAGGAGAA GCTCCGGCAG 
TCACTTAGCG TTCGCCTGGA GGTGGCCAAG TTCTTGCAGC AGACCCTAGA TCAGATGCCC 
GTCCAGCACA AGGAACACAG CAGCGAAGAG GCCAAGCAGT TCGAGGCCTT TTTCACTAAA 
ATCCGGAATC CCACTGAACC AGTGAGCAAC GATGAAATCA TCAAATTCGC CAAGCGATTC 
GACGATGAGA TCACCTTAGA TTCACTGTCC CGGGAGCAAT TGGCGGCTCT TTGTCGCGTC 
CTTGAACTGA ACACAATTGG CACCACCACG CTGCTGCGGT TCCAGCTGCG TCTGAAGCTG 
AGATCTTTGG CCACCGATGA CCGCGTTATT GCCCGCGAAG GAGTCGACTC CCTGGACCTT 
CTAGAGCTGC AACAGGCGTG CAAGGCACGT GGAATGCGGG CATATGGCCT AACGGAGGAG 
CGTCTTCGTT TTCAGCTTAA GGAGTGGATA GATCTCTCCC TGAACGAACA GGTACCGCCT 
ACGCTGCTGC TCCTGTCAAG AACCATGCTT ATTTCCGACG ACAGCATCAC CACCGATAAG 
CTCAAGGAGA CGATTCGTGT GCTACCGGAT GCAGTGGGTG CCCACACACG GCACGCTATC 
GGGGAGAGTG AGGGCAAGGT GGACAACAAG ACGAAGATCG AAATCATCAA GGAGGAGGAG 
CGCAAGATTC GCGAAGAGCG CGAGGAAGAG CGGGAAGAGA CCATCGCAAA ACGATCGGCC 
ATCAAGGAGG AGATTCCCGC TCCTTATGTA TTTGCTGAGA AGTTGTCGGG TTCCCAGGAT 
CTTCTGGATC ACAAGGAACA GTCCTCGGTG TCCGAGACGG ACAAGGGCAT TTCCTCTACC 
GATGTACAGC TGCTGTCTGA GGCCCTAAAG ACACTCTCAT CTGATAAACA GCTCGTGGTG 
GAAAAGGAAA CAATCAAGGA ACTCAAGGAG GAATTGGCCG ACTATAAGGA GGATGTGGAG 
GAGCTACGCG AGGTGCGACA GGTAGTCAAG GAGCCTGTGC GCGAAAGCCG GGCGGCCAAG 
TTGCTCTACA ATCGGGTCAA CAAGATGATT TCCCAACTCG ATAATGTGCT TAACGACCTA 
GAGGCCAGGC AGCATCAAAT CAAGCAGGCG GAATCCTCTG ATTACGCGGC GTCCAGTCCC 
ACTGTGGAAC CACAGCAAAT GGTGCACATT GACGAGCTGG TCGCCACCAT CCGGCGAATG 
AAGGAGGCAT CCGATGAAGA GCGATTCAAG GTTGTCGGGG ATCTGCTGGT CAAACTCGAC 
GCGGACAAAG ACGGTGTGAT TTCGGTGAAC GAGATCACCA AGGCGGTGCA GAGCATCGAC 
CGCGAGGCCA CGAATATCGA CAAGAAGCAG CTGGAGGAGT TTACCGAGCT ACTTTCCAAG 
TTGGCGTCCC GGCGACGACA CGAGGAGATC GTGCACATCG ACGATTTAAT GAATAACATC 
AAGGTGTTGA AGGAAACTAG CGATGAGGCA CGACTTAAGC ACATCGAAGC TGTACTGGAG 
AAATTTGATG CCGACAAGGA TGGCGTGGTG ACCGTCAACG ATATTCGTAA GGTGTTGGAA 
TCCATTGGTC GTGATAACAT CAAGCTGAGC GACAAGGCCA TTGAGGAACT CATCAGTCTG 
CTTGACAAGG AGCAAGTACT GCAGGCAGAG CAGAAGATTG AGAAGGCCAT TGCTAAGAGC 
ATGAAAGAGG CTGAGAAACT GAAGTCTGAG GTGGACAAAG CGGATAAGGA CTTGTCCAAG 
CTTGTTAATG ATATTCACGA TTCCGCCAAG GAGATTCAGG ATATTGCCAA CGAGATGCGT 
GATAAGGAGG AAACTGTGCC CGATAAAGCG AAGGAGCTAA AAGCAGAGCC CGCATTCAAG 
GATACGGCCA AAACATTGAA GGACAACGCA AAGGACCTGG ACGATTTAGC GAAGGACCCA 
AAGTCAGACC CCAAATCCCC GACAAAGGCT TCTACAGGGT CCGGTCCAGC CGGACTGTCA 
GGTGGAGGCC CCAGCAGTGG CAGTAGCGGT ATTGCCACCG GATCAACGAC GGAATCAGCG 
CTCCGAGAGG CGGCCGAGCG TCAAATGGAA AAGATCCTGC CCTCCACAGA CATTGGTCTG 
CCCCCCACGA TACAAACACC ATCGCAACCA CCGACGTCAA AAAAAGCAAC GGCCACTGCG 
TCGACCCTGT CCACGACGAT AACGGCCAAG AAACTGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611922.1
CDS	353..3394
Translation	MNALLRHKGRNLRTSHLAQNVYKRFLKSNCCACSSVNVTDEPAKEDELPRRSASTSVLELSRSLGTYRRFQPHANYGYDYSGYGFRHLHTSRTLLETSSSKIDATVKKLKNQQKEKVEEIMKEVANGQAAAVRASSAATATASSEKGQNASATAGSTSATASTTSLAKTADKSVAKPKKPLRTRIWDELVHYYHGFRLLFIDVAICSKLLWRVLNGKTLTRRENKQLQRTTSDLFRLIPFSVFIIVPFMELLLPLFIKFFPGMLPSTFQTSTDRQEKLRQSLSVRLEVAKFLQQTLDQMPVQHKEHSSEEAKQFEAFFTKIRNPTEPVSNDEIIKFAKRFDDEITLDSLSREQLAALCRVLELNTIGTTTLLRFQLRLKLRSLATDDRVIAREGVDSLDLLELQQACKARGMRAYGLTEERLRFQLKEWIDLSLNEQVPPTLLLLSRTMLISDDSITTDKLKETIRVLPDAVGAHTRHAIGESEGKVDNKTKIEIIKEEERKIREEREEEREETIAKRSAIKEEIPAPYVFAEKLSGSQDLLDHKEQSSVSETDKGISSTDVQLLSEALKTLSSDKQLVVEKETIKELKEELADYKEDVEELREVRQVVKEPVRESRAAKLLYNRVNKMISQLDNVLNDLEARQHQIKQAESSDYAASSPTVEPQQMVHIDELVATIRRMKEASDEERFKVVGDLLVKLDADKDGVISVNEITKAVQSIDREATNIDKKQLEEFTELLSKLASRRRHEEIVHIDDLMNNIKVLKETSDEARLKHIEAVLEKFDADKDGVVTVNDIRKVLESIGRDNIKLSDKAIEELISLLDKEQVLQAEQKIEKAIAKSMKEAEKLKSEVDKADKDLSKLVNDIHDSAKEIQDIANEMRDKEETVPDKAKELKAEPAFKDTAKTLKDNAKDLDDLAKDPKSDPKSPTKASTGSGPAGLSGGGPSSGSSGIATGSTTESALREAAERQMEKILPSTDIGLPPTIQTPSQPPTSKKATATASTLSTTITAKKLL

Target ORF information:

RefSeq Version	NM_138078.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster leucine zipper and EF-hand containing transmembrane protein 1, transcript variant B (Letm1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138078.3

ORF Insert Sequence:

ATGAACGCCC TGCTGCGTCA CAAGGGACGC AATCTGCGGA CGAGTCACTT GGCCCAGAAT 
GTCTACAAGC GCTTTCTTAA ATCGAACTGT TGCGCATGCA GTTCGGTGAA CGTCACAGAT 
GAACCCGCCA AAGAAGATGA ACTACCGCGA AGATCTGCTT CGACCTCGGT GCTGGAGCTA 
AGCAGATCGC TTGGCACTTA CCGAAGGTTT CAGCCACATG CCAACTATGG ATACGACTAC 
TCCGGCTATG GATTTAGGCA TCTGCATACG AGCCGGACTT TGCTGGAGAC GTCCAGCTCA 
AAGATCGACG CAACAGTCAA GAAGCTGAAA AACCAGCAGA AGGAGAAGGT GGAGGAGATC 
ATGAAGGAGG TGGCCAATGG ACAGGCCGCG GCAGTTCGTG CCAGCAGTGC CGCTACCGCC 
ACGGCCTCCT CGGAGAAGGG TCAAAACGCT AGTGCGACCG CGGGCTCCAC GTCGGCGACT 
GCCTCAACGA CGTCTTTGGC CAAGACCGCA GACAAGTCGG TGGCCAAGCC AAAGAAACCA 
CTCCGCACAC GGATTTGGGA TGAGCTGGTG CACTATTACC ACGGCTTTAG GCTGCTCTTC 
ATCGATGTGG CAATTTGCTC TAAGCTCCTT TGGCGAGTGC TCAACGGCAA GACGCTCACG 
CGGCGCGAGA ACAAGCAGCT TCAGCGGACC ACCTCTGACT TGTTCCGTCT GATCCCCTTT 
TCGGTGTTCA TCATCGTGCC CTTCATGGAG CTGCTGCTGC CGTTGTTCAT AAAATTCTTC 
CCCGGCATGC TGCCCTCCAC GTTCCAGACT TCGACCGATC GACAGGAGAA GCTCCGGCAG 
TCACTTAGCG TTCGCCTGGA GGTGGCCAAG TTCTTGCAGC AGACCCTAGA TCAGATGCCC 
GTCCAGCACA AGGAACACAG CAGCGAAGAG GCCAAGCAGT TCGAGGCCTT TTTCACTAAA 
ATCCGGAATC CCACTGAACC AGTGAGCAAC GATGAAATCA TCAAATTCGC CAAGCGATTC 
GACGATGAGA TCACCTTAGA TTCACTGTCC CGGGAGCAAT TGGCGGCTCT TTGTCGCGTC 
CTTGAACTGA ACACAATTGG CACCACCACG CTGCTGCGGT TCCAGCTGCG TCTGAAGCTG 
AGATCTTTGG CCACCGATGA CCGCGTTATT GCCCGCGAAG GAGTCGACTC CCTGGACCTT 
CTAGAGCTGC AACAGGCGTG CAAGGCACGT GGAATGCGGG CATATGGCCT AACGGAGGAG 
CGTCTTCGTT TTCAGCTTAA GGAGTGGATA GATCTCTCCC TGAACGAACA GGTACCGCCT 
ACGCTGCTGC TCCTGTCAAG AACCATGCTT ATTTCCGACG ACAGCATCAC CACCGATAAG 
CTCAAGGAGA CGATTCGTGT GCTACCGGAT GCAGTGGGTG CCCACACACG GCACGCTATC 
GGGGAGAGTG AGGGCAAGGT GGACAACAAG ACGAAGATCG AAATCATCAA GGAGGAGGAG 
CGCAAGATTC GCGAAGAGCG CGAGGAAGAG CGGGAAGAGA CCATCGCAAA ACGATCGGCC 
ATCAAGGAGG AGATTCCCGC TCCTTATGTA TTTGCTGAGA AGTTGTCGGG TTCCCAGGAT 
CTTCTGGATC ACAAGGAACA GTCCTCGGTG TCCGAGACGG ACAAGGGCAT TTCCTCTACC 
GATGTACAGC TGCTGTCTGA GGCCCTAAAG ACACTCTCAT CTGATAAACA GCTCGTGGTG 
GAAAAGGAAA CAATCAAGGA ACTCAAGGAG GAATTGGCCG ACTATAAGGA GGATGTGGAG 
GAGCTACGCG AGGTGCGACA GGTAGTCAAG GAGCCTGTGC GCGAAAGCCG GGCGGCCAAG 
TTGCTCTACA ATCGGGTCAA CAAGATGATT TCCCAACTCG ATAATGTGCT TAACGACCTA 
GAGGCCAGGC AGCATCAAAT CAAGCAGGCG GAATCCTCTG ATTACGCGGC GTCCAGTCCC 
ACTGTGGAAC CACAGCAAAT GGTGCACATT GACGAGCTGG TCGCCACCAT CCGGCGAATG 
AAGGAGGCAT CCGATGAAGA GCGATTCAAG GTTGTCGGGG ATCTGCTGGT CAAACTCGAC 
GCGGACAAAG ACGGTGTGAT TTCGGTGAAC GAGATCACCA AGGCGGTGCA GAGCATCGAC 
CGCGAGGCCA CGAATATCGA CAAGAAGCAG CTGGAGGAGT TTACCGAGCT ACTTTCCAAG 
TTGGCGTCCC GGCGACGACA CGAGGAGATC GTGCACATCG ACGATTTAAT GAATAACATC 
AAGGTGTTGA AGGAAACTAG CGATGAGGCA CGACTTAAGC ACATCGAAGC TGTACTGGAG 
AAATTTGATG CCGACAAGGA TGGCGTGGTG ACCGTCAACG ATATTCGTAA GGTGTTGGAA 
TCCATTGGTC GTGATAACAT CAAGCTGAGC GACAAGGCCA TTGAGGAACT CATCAGTCTG 
CTTGACAAGG AGCAAGTACT GCAGGCAGAG CAGAAGATTG AGAAGGCCAT TGCTAAGAGC 
ATGAAAGAGG CTGAGAAACT GAAGTCTGAG GTGGACAAAG CGGATAAGGA CTTGTCCAAG 
CTTGTTAATG ATATTCACGA TTCCGCCAAG GAGATTCAGG ATATTGCCAA CGAGATGCGT 
GATAAGGAGG AAACTGTGCC CGATAAAGCG AAGGAGCTAA AAGCAGAGCC CGCATTCAAG 
GATACGGCCA AAACATTGAA GGACAACGCA AAGGACCTGG ACGATTTAGC GAAGGACCCA 
AAGTCAGACC CCAAATCCCC GACAAAGGCT TCTACAGGGT CCGGTCCAGC CGGACTGTCA 
GGTGGAGGCC CCAGCAGTGG CAGTAGCGGT ATTGCCACCG GATCAACGAC GGAATCAGCG 
CTCCGAGAGG CGGCCGAGCG TCAAATGGAA AAGATCCTGC CCTCCACAGA CATTGGTCTG 
CCCCCCACGA TACAAACACC ATCGCAACCA CCGACGTCAA AAAAAGCAAC GGCCACTGCG 
TCGACCCTGT CCACGACGAT AACGGCCAAG AAACTGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Letm1 ( NM_166674.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726453.1 Drosophila melanogaster leucine zipper and EF-hand containing transmembrane protein 1, transcript variant A (Letm1), mRNA.

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CloneID	OFa11183
Clone ID Related Accession (Same CDS sequence)	NM_166675.2 , NM_138078.3 , NM_166674.2
Accession Version	NM_166674.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	leucine zipper and EF-hand containing transmembrane protein 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_166674.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACGCCC TGCTGCGTCA CAAGGGACGC AATCTGCGGA CGAGTCACTT GGCCCAGAAT 
GTCTACAAGC GCTTTCTTAA ATCGAACTGT TGCGCATGCA GTTCGGTGAA CGTCACAGAT 
GAACCCGCCA AAGAAGATGA ACTACCGCGA AGATCTGCTT CGACCTCGGT GCTGGAGCTA 
AGCAGATCGC TTGGCACTTA CCGAAGGTTT CAGCCACATG CCAACTATGG ATACGACTAC 
TCCGGCTATG GATTTAGGCA TCTGCATACG AGCCGGACTT TGCTGGAGAC GTCCAGCTCA 
AAGATCGACG CAACAGTCAA GAAGCTGAAA AACCAGCAGA AGGAGAAGGT GGAGGAGATC 
ATGAAGGAGG TGGCCAATGG ACAGGCCGCG GCAGTTCGTG CCAGCAGTGC CGCTACCGCC 
ACGGCCTCCT CGGAGAAGGG TCAAAACGCT AGTGCGACCG CGGGCTCCAC GTCGGCGACT 
GCCTCAACGA CGTCTTTGGC CAAGACCGCA GACAAGTCGG TGGCCAAGCC AAAGAAACCA 
CTCCGCACAC GGATTTGGGA TGAGCTGGTG CACTATTACC ACGGCTTTAG GCTGCTCTTC 
ATCGATGTGG CAATTTGCTC TAAGCTCCTT TGGCGAGTGC TCAACGGCAA GACGCTCACG 
CGGCGCGAGA ACAAGCAGCT TCAGCGGACC ACCTCTGACT TGTTCCGTCT GATCCCCTTT 
TCGGTGTTCA TCATCGTGCC CTTCATGGAG CTGCTGCTGC CGTTGTTCAT AAAATTCTTC 
CCCGGCATGC TGCCCTCCAC GTTCCAGACT TCGACCGATC GACAGGAGAA GCTCCGGCAG 
TCACTTAGCG TTCGCCTGGA GGTGGCCAAG TTCTTGCAGC AGACCCTAGA TCAGATGCCC 
GTCCAGCACA AGGAACACAG CAGCGAAGAG GCCAAGCAGT TCGAGGCCTT TTTCACTAAA 
ATCCGGAATC CCACTGAACC AGTGAGCAAC GATGAAATCA TCAAATTCGC CAAGCGATTC 
GACGATGAGA TCACCTTAGA TTCACTGTCC CGGGAGCAAT TGGCGGCTCT TTGTCGCGTC 
CTTGAACTGA ACACAATTGG CACCACCACG CTGCTGCGGT TCCAGCTGCG TCTGAAGCTG 
AGATCTTTGG CCACCGATGA CCGCGTTATT GCCCGCGAAG GAGTCGACTC CCTGGACCTT 
CTAGAGCTGC AACAGGCGTG CAAGGCACGT GGAATGCGGG CATATGGCCT AACGGAGGAG 
CGTCTTCGTT TTCAGCTTAA GGAGTGGATA GATCTCTCCC TGAACGAACA GGTACCGCCT 
ACGCTGCTGC TCCTGTCAAG AACCATGCTT ATTTCCGACG ACAGCATCAC CACCGATAAG 
CTCAAGGAGA CGATTCGTGT GCTACCGGAT GCAGTGGGTG CCCACACACG GCACGCTATC 
GGGGAGAGTG AGGGCAAGGT GGACAACAAG ACGAAGATCG AAATCATCAA GGAGGAGGAG 
CGCAAGATTC GCGAAGAGCG CGAGGAAGAG CGGGAAGAGA CCATCGCAAA ACGATCGGCC 
ATCAAGGAGG AGATTCCCGC TCCTTATGTA TTTGCTGAGA AGTTGTCGGG TTCCCAGGAT 
CTTCTGGATC ACAAGGAACA GTCCTCGGTG TCCGAGACGG ACAAGGGCAT TTCCTCTACC 
GATGTACAGC TGCTGTCTGA GGCCCTAAAG ACACTCTCAT CTGATAAACA GCTCGTGGTG 
GAAAAGGAAA CAATCAAGGA ACTCAAGGAG GAATTGGCCG ACTATAAGGA GGATGTGGAG 
GAGCTACGCG AGGTGCGACA GGTAGTCAAG GAGCCTGTGC GCGAAAGCCG GGCGGCCAAG 
TTGCTCTACA ATCGGGTCAA CAAGATGATT TCCCAACTCG ATAATGTGCT TAACGACCTA 
GAGGCCAGGC AGCATCAAAT CAAGCAGGCG GAATCCTCTG ATTACGCGGC GTCCAGTCCC 
ACTGTGGAAC CACAGCAAAT GGTGCACATT GACGAGCTGG TCGCCACCAT CCGGCGAATG 
AAGGAGGCAT CCGATGAAGA GCGATTCAAG GTTGTCGGGG ATCTGCTGGT CAAACTCGAC 
GCGGACAAAG ACGGTGTGAT TTCGGTGAAC GAGATCACCA AGGCGGTGCA GAGCATCGAC 
CGCGAGGCCA CGAATATCGA CAAGAAGCAG CTGGAGGAGT TTACCGAGCT ACTTTCCAAG 
TTGGCGTCCC GGCGACGACA CGAGGAGATC GTGCACATCG ACGATTTAAT GAATAACATC 
AAGGTGTTGA AGGAAACTAG CGATGAGGCA CGACTTAAGC ACATCGAAGC TGTACTGGAG 
AAATTTGATG CCGACAAGGA TGGCGTGGTG ACCGTCAACG ATATTCGTAA GGTGTTGGAA 
TCCATTGGTC GTGATAACAT CAAGCTGAGC GACAAGGCCA TTGAGGAACT CATCAGTCTG 
CTTGACAAGG AGCAAGTACT GCAGGCAGAG CAGAAGATTG AGAAGGCCAT TGCTAAGAGC 
ATGAAAGAGG CTGAGAAACT GAAGTCTGAG GTGGACAAAG CGGATAAGGA CTTGTCCAAG 
CTTGTTAATG ATATTCACGA TTCCGCCAAG GAGATTCAGG ATATTGCCAA CGAGATGCGT 
GATAAGGAGG AAACTGTGCC CGATAAAGCG AAGGAGCTAA AAGCAGAGCC CGCATTCAAG 
GATACGGCCA AAACATTGAA GGACAACGCA AAGGACCTGG ACGATTTAGC GAAGGACCCA 
AAGTCAGACC CCAAATCCCC GACAAAGGCT TCTACAGGGT CCGGTCCAGC CGGACTGTCA 
GGTGGAGGCC CCAGCAGTGG CAGTAGCGGT ATTGCCACCG GATCAACGAC GGAATCAGCG 
CTCCGAGAGG CGGCCGAGCG TCAAATGGAA AAGATCCTGC CCTCCACAGA CATTGGTCTG 
CCCCCCACGA TACAAACACC ATCGCAACCA CCGACGTCAA AAAAAGCAAC GGCCACTGCG 
TCGACCCTGT CCACGACGAT AACGGCCAAG AAACTGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726453.1
CDS	353..3394
Translation	MNALLRHKGRNLRTSHLAQNVYKRFLKSNCCACSSVNVTDEPAKEDELPRRSASTSVLELSRSLGTYRRFQPHANYGYDYSGYGFRHLHTSRTLLETSSSKIDATVKKLKNQQKEKVEEIMKEVANGQAAAVRASSAATATASSEKGQNASATAGSTSATASTTSLAKTADKSVAKPKKPLRTRIWDELVHYYHGFRLLFIDVAICSKLLWRVLNGKTLTRRENKQLQRTTSDLFRLIPFSVFIIVPFMELLLPLFIKFFPGMLPSTFQTSTDRQEKLRQSLSVRLEVAKFLQQTLDQMPVQHKEHSSEEAKQFEAFFTKIRNPTEPVSNDEIIKFAKRFDDEITLDSLSREQLAALCRVLELNTIGTTTLLRFQLRLKLRSLATDDRVIAREGVDSLDLLELQQACKARGMRAYGLTEERLRFQLKEWIDLSLNEQVPPTLLLLSRTMLISDDSITTDKLKETIRVLPDAVGAHTRHAIGESEGKVDNKTKIEIIKEEERKIREEREEEREETIAKRSAIKEEIPAPYVFAEKLSGSQDLLDHKEQSSVSETDKGISSTDVQLLSEALKTLSSDKQLVVEKETIKELKEELADYKEDVEELREVRQVVKEPVRESRAAKLLYNRVNKMISQLDNVLNDLEARQHQIKQAESSDYAASSPTVEPQQMVHIDELVATIRRMKEASDEERFKVVGDLLVKLDADKDGVISVNEITKAVQSIDREATNIDKKQLEEFTELLSKLASRRRHEEIVHIDDLMNNIKVLKETSDEARLKHIEAVLEKFDADKDGVVTVNDIRKVLESIGRDNIKLSDKAIEELISLLDKEQVLQAEQKIEKAIAKSMKEAEKLKSEVDKADKDLSKLVNDIHDSAKEIQDIANEMRDKEETVPDKAKELKAEPAFKDTAKTLKDNAKDLDDLAKDPKSDPKSPTKASTGSGPAGLSGGGPSSGSSGIATGSTTESALREAAERQMEKILPSTDIGLPPTIQTPSQPPTSKKATATASTLSTTITAKKLL

Target ORF information:

RefSeq Version	NM_166674.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster leucine zipper and EF-hand containing transmembrane protein 1, transcript variant A (Letm1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166674.2

ORF Insert Sequence:

ATGAACGCCC TGCTGCGTCA CAAGGGACGC AATCTGCGGA CGAGTCACTT GGCCCAGAAT 
GTCTACAAGC GCTTTCTTAA ATCGAACTGT TGCGCATGCA GTTCGGTGAA CGTCACAGAT 
GAACCCGCCA AAGAAGATGA ACTACCGCGA AGATCTGCTT CGACCTCGGT GCTGGAGCTA 
AGCAGATCGC TTGGCACTTA CCGAAGGTTT CAGCCACATG CCAACTATGG ATACGACTAC 
TCCGGCTATG GATTTAGGCA TCTGCATACG AGCCGGACTT TGCTGGAGAC GTCCAGCTCA 
AAGATCGACG CAACAGTCAA GAAGCTGAAA AACCAGCAGA AGGAGAAGGT GGAGGAGATC 
ATGAAGGAGG TGGCCAATGG ACAGGCCGCG GCAGTTCGTG CCAGCAGTGC CGCTACCGCC 
ACGGCCTCCT CGGAGAAGGG TCAAAACGCT AGTGCGACCG CGGGCTCCAC GTCGGCGACT 
GCCTCAACGA CGTCTTTGGC CAAGACCGCA GACAAGTCGG TGGCCAAGCC AAAGAAACCA 
CTCCGCACAC GGATTTGGGA TGAGCTGGTG CACTATTACC ACGGCTTTAG GCTGCTCTTC 
ATCGATGTGG CAATTTGCTC TAAGCTCCTT TGGCGAGTGC TCAACGGCAA GACGCTCACG 
CGGCGCGAGA ACAAGCAGCT TCAGCGGACC ACCTCTGACT TGTTCCGTCT GATCCCCTTT 
TCGGTGTTCA TCATCGTGCC CTTCATGGAG CTGCTGCTGC CGTTGTTCAT AAAATTCTTC 
CCCGGCATGC TGCCCTCCAC GTTCCAGACT TCGACCGATC GACAGGAGAA GCTCCGGCAG 
TCACTTAGCG TTCGCCTGGA GGTGGCCAAG TTCTTGCAGC AGACCCTAGA TCAGATGCCC 
GTCCAGCACA AGGAACACAG CAGCGAAGAG GCCAAGCAGT TCGAGGCCTT TTTCACTAAA 
ATCCGGAATC CCACTGAACC AGTGAGCAAC GATGAAATCA TCAAATTCGC CAAGCGATTC 
GACGATGAGA TCACCTTAGA TTCACTGTCC CGGGAGCAAT TGGCGGCTCT TTGTCGCGTC 
CTTGAACTGA ACACAATTGG CACCACCACG CTGCTGCGGT TCCAGCTGCG TCTGAAGCTG 
AGATCTTTGG CCACCGATGA CCGCGTTATT GCCCGCGAAG GAGTCGACTC CCTGGACCTT 
CTAGAGCTGC AACAGGCGTG CAAGGCACGT GGAATGCGGG CATATGGCCT AACGGAGGAG 
CGTCTTCGTT TTCAGCTTAA GGAGTGGATA GATCTCTCCC TGAACGAACA GGTACCGCCT 
ACGCTGCTGC TCCTGTCAAG AACCATGCTT ATTTCCGACG ACAGCATCAC CACCGATAAG 
CTCAAGGAGA CGATTCGTGT GCTACCGGAT GCAGTGGGTG CCCACACACG GCACGCTATC 
GGGGAGAGTG AGGGCAAGGT GGACAACAAG ACGAAGATCG AAATCATCAA GGAGGAGGAG 
CGCAAGATTC GCGAAGAGCG CGAGGAAGAG CGGGAAGAGA CCATCGCAAA ACGATCGGCC 
ATCAAGGAGG AGATTCCCGC TCCTTATGTA TTTGCTGAGA AGTTGTCGGG TTCCCAGGAT 
CTTCTGGATC ACAAGGAACA GTCCTCGGTG TCCGAGACGG ACAAGGGCAT TTCCTCTACC 
GATGTACAGC TGCTGTCTGA GGCCCTAAAG ACACTCTCAT CTGATAAACA GCTCGTGGTG 
GAAAAGGAAA CAATCAAGGA ACTCAAGGAG GAATTGGCCG ACTATAAGGA GGATGTGGAG 
GAGCTACGCG AGGTGCGACA GGTAGTCAAG GAGCCTGTGC GCGAAAGCCG GGCGGCCAAG 
TTGCTCTACA ATCGGGTCAA CAAGATGATT TCCCAACTCG ATAATGTGCT TAACGACCTA 
GAGGCCAGGC AGCATCAAAT CAAGCAGGCG GAATCCTCTG ATTACGCGGC GTCCAGTCCC 
ACTGTGGAAC CACAGCAAAT GGTGCACATT GACGAGCTGG TCGCCACCAT CCGGCGAATG 
AAGGAGGCAT CCGATGAAGA GCGATTCAAG GTTGTCGGGG ATCTGCTGGT CAAACTCGAC 
GCGGACAAAG ACGGTGTGAT TTCGGTGAAC GAGATCACCA AGGCGGTGCA GAGCATCGAC 
CGCGAGGCCA CGAATATCGA CAAGAAGCAG CTGGAGGAGT TTACCGAGCT ACTTTCCAAG 
TTGGCGTCCC GGCGACGACA CGAGGAGATC GTGCACATCG ACGATTTAAT GAATAACATC 
AAGGTGTTGA AGGAAACTAG CGATGAGGCA CGACTTAAGC ACATCGAAGC TGTACTGGAG 
AAATTTGATG CCGACAAGGA TGGCGTGGTG ACCGTCAACG ATATTCGTAA GGTGTTGGAA 
TCCATTGGTC GTGATAACAT CAAGCTGAGC GACAAGGCCA TTGAGGAACT CATCAGTCTG 
CTTGACAAGG AGCAAGTACT GCAGGCAGAG CAGAAGATTG AGAAGGCCAT TGCTAAGAGC 
ATGAAAGAGG CTGAGAAACT GAAGTCTGAG GTGGACAAAG CGGATAAGGA CTTGTCCAAG 
CTTGTTAATG ATATTCACGA TTCCGCCAAG GAGATTCAGG ATATTGCCAA CGAGATGCGT 
GATAAGGAGG AAACTGTGCC CGATAAAGCG AAGGAGCTAA AAGCAGAGCC CGCATTCAAG 
GATACGGCCA AAACATTGAA GGACAACGCA AAGGACCTGG ACGATTTAGC GAAGGACCCA 
AAGTCAGACC CCAAATCCCC GACAAAGGCT TCTACAGGGT CCGGTCCAGC CGGACTGTCA 
GGTGGAGGCC CCAGCAGTGG CAGTAGCGGT ATTGCCACCG GATCAACGAC GGAATCAGCG 
CTCCGAGAGG CGGCCGAGCG TCAAATGGAA AAGATCCTGC CCTCCACAGA CATTGGTCTG 
CCCCCCACGA TACAAACACC ATCGCAACCA CCGACGTCAA AAAAAGCAAC GGCCACTGCG 
TCGACCCTGT CCACGACGAT AACGGCCAAG AAACTGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Letm1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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