Notum cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Notum
Entrez Gene ID	39751
Full Name	CG13076 gene product from transcript CG13076-RA
Synonyms	CG13076,Dmel\CG13076,Not,Nt,Wf,anon-WO0118547.482,anon-WO03062410.10,anon-WO03062410.8,l(3)72CDf,l(3)72Da,notum,ntm,wf
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 72C3-72D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_168643.3	NP_730096.2	notum

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NOTUM	XP_003315832.1
Canis lupus familiaris (dog)	NOTUM	XP_540493.3
Homo sapiens (human)	NOTUM	NP_848588.3
Bos taurus (cattle)	NOTUM	XP_003587522.2
Rattus norvegicus (Norway rat)	Notum	NP_001013997.2
Gallus gallus (chicken)	NOTUM	XP_415640.4
Drosophila melanogaster (fruit fly)	Notum	NP_730096.2
Arabidopsis thaliana (thale cress)	AT5G45280	NP_199341.1
Oryza sativa (rice)	Os04g0602500	NP_001053773.1
	NOTUM	XP_001112829.2
Mus musculus (house mouse)	Notum	NP_780472.3
Arabidopsis thaliana (thale cress)	AT4G19410	NP_567585.1
Xenopus tropicalis (tropical clawed frog)	notum	NP_001120228.2
Danio rerio (zebrafish)	notum1a	NP_001155126.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Notum normally functions to coordinate synaptic structural and functional differentiation via negative regulation of Wg trans-synaptic signaling in the extracellular synaptomatrix.
Title: Notum coordinates synapse development via extracellular regulation of Wingless trans-synaptic signaling.

Notum requires glypicans to suppress Wnt signalling, but does not cleave their glycophosphatidylinositol anchor; structural analyses reveal glycosaminoglycan binding sites on Notum, which probably help Notum to co-localize with Wnt proteins
Title: Notum deacylates Wnt proteins to suppress signalling activity.

Hydrolysis of the Dally-GPI by Notum provides a switch from low to high level signalling by promoting internalisation of the Hh-Patched ligand-receptor complex.
Title: Dally and Notum regulate the switch between low and high level Hedgehog pathway signalling.

the long-range activity of Hedgehog is regulated in the apical extracellular space by the glypican Dally and the hydrolase Notum
Title: The long-range activity of Hedgehog is regulated in the apical extracellular space by the glypican Dally and the hydrolase Notum.

notum can cleave mammalian glypticans and GPI-anchored proteins from the cell surface.
Title: Mammalian Notum induces the release of glypicans and other GPI-anchored proteins from the cell surface.

The following Notum gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Notum cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa20968	NM_168643.3 Latest version!	Drosophila melanogaster notum (Notum), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Notum ( NM_168643.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730096.2 Drosophila melanogaster notum (Notum), mRNA.

Price & Availability↑

CloneID	OFa20968
Clone ID Related Accession (Same CDS sequence)	NM_168643.3
Accession Version	NM_168643.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	notum
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168643.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGTCG AACAAATTGA TAAAATGGCA GCTAAAGCCG GAGAAGCTAC AAATAAATGG 
ATTAAGCCAC AGCAGCCGCT GCTAACGCTT CTGCTGCTCT TAGCCACGTT CAGTCAGCTG 
CCAGCCGTTT GCAGCAGCAG CATCTTGGAT GCGGCGAGTC TGCAGGAGAA GGATCCATTG 
AGGGACACCA GCATGAACAT GATCCAGCGC AACTACATGG TGATGCACTC GGCCAGCGGC 
TCCGGTGACC ACAGCCGCTC CCTGAAACGC GCCAATCTGG CCAACACGAG CATCACCTGC 
AACGATGGCA GTCATGCCGG TTTCTATCTG CGCAAGCATC CCAGCTCCAA GAAGTGGATC 
GTTCTACTCG AAGGTGGCTG GCACTGTTTC GATGTGCGCT CGTGCCGCTC CAGATGGATG 
CGACTGCGCC ACCTAATGAC CTCCTCCCAG TGGCCAGAGA CGAGAGATGT TGGCGGCATT 
CTATCGCCAC ATCCCGAGGA GAATCCCTAC TGGCACAATG CCAACCATGT GCTCATCCCG 
TACTGCAGCA GTGATTCGTG GTCGGGAACA CGAACGGAGC CGGATACCAG CGATCGGGAG 
AACAGTTGGC GGTTCATGGG TGCCCTGATT CTGCGCCAGG TGATCGCCGA GCTGATTCCC 
GTGGGATTGG GCCGAGTGCC GGGTGGCGAA CTGATGCTCG TTGGCTCATC GGCTGGTGGC 
ATGGGTGTAA TGCTCAATTT GGATCGCATT CGAGACTTTC TGGTCAACGA GAAAAAGCTA 
CAGATCACAG TGAGGGGAGT GAGTGACTCG GGTTGGTTCC TGGACAGAGA GCCATACACG 
CCGGCGGCCG TGGCCTCCAA TGAGGCAGTG CGTCAGGGTT GGAAGTTGTG GCAGGGCTTG 
CTGCCCGAGG AGTGCACCAA AAGTTATCCC ACGGAGCCAT GGAGATGTTA CTATGGATAC 
CGACTATATC CCACTTTGAA AACTCCGCTT TTCGTTTTCC AATGGCTTTT CGACGAGGCC 
CAAATGCGAG TGGATAATGT TGGAGCACCC GTGACGCCTC AGCAATGGAA CTACATCCAC 
GAAATGGGCG GCGCCCTGAG ATCCTCGCTG GACAATGTGA GCGCAGTGTT TGCACCCAGT 
TGCATAGGTC ATGGGGTGCT CTTCAAACGG GATTGGGTCA ATATCAAGAT CGACGATATA 
TCGCTGCCCT CAGCGCTGCG CTGTTGGGAG CACTCAACGC GCAGTAGGCG TCATGACAAG 
CTCAAGCGTT CCACGGAACC ATCGACGGCG GTCTCGCATC CAGAACATGC CAATAACCAA 
AGACACCAGC GACACCGTCA GCGGCTGCAG CGACAGAAGC ATAACAATGT GGCCCAATCT 
GGAGGGCAGC AGCGCAAGCA CAATCACCTG AGCAAGGAGG AGCGAGAGGA GCGAAAACGA 
TTGCGCCAGG AGCAGCGACA GAGGCGGAAG CAACGTCGCC GCCAGCAGCA GCAAAAGAAG 
GCCAATGGCG GGCAGGAGCA TCGCAATAAG AAGGACAACA GCCCCAAGTC GAGCAATGGC 
AATGATCAGC GGAAACAGCG ACGGCGCCAG CAATTGACAG CCGAGGAAAG GCAGGAGCAG 
CGCAAGCGAC GCAGGAAGGC TCAGCAGCAG CAGATGAAAA TGCAGCGGGA ACAGCCGGCG 
GCGGGTGTTT TCCTGGAAGC GAGTGCGCCC CAGAAGACAC GCTCCTCCAA CAACGCCTCC 
GCGGGCACAA AGTCCAAGAA GCGACATCGC GTTCCGCGAG TGCCAGAGAA ATGCGGCCTC 
CGTCTGCTGG AACGCTGCAG TTGGCCGCAA TGCAATCACT CGTGTCCCAC GCTCACCAAT 
CCCATGACCG GCGAGGAGAT GCGATTCCTC GAGCTGCTCA CCGCCTTTGG CTTGGACATC 
GAGGCGGTGG CCGCCGCTCT GGGAGTCGAT ATGCACACGC TCAACAACAT GGAGCGCACC 
GAGTTGGTCA ACATGCTCAC CCAGCAGGCT AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730096.2
CDS	366..2381
Translation	MAVEQIDKMAAKAGEATNKWIKPQQPLLTLLLLLATFSQLPAVCSSSILDAASLQEKDPLRDTSMNMIQRNYMVMHSASGSGDHSRSLKRANLANTSITCNDGSHAGFYLRKHPSSKKWIVLLEGGWHCFDVRSCRSRWMRLRHLMTSSQWPETRDVGGILSPHPEENPYWHNANHVLIPYCSSDSWSGTRTEPDTSDRENSWRFMGALILRQVIAELIPVGLGRVPGGELMLVGSSAGGMGVMLNLDRIRDFLVNEKKLQITVRGVSDSGWFLDREPYTPAAVASNEAVRQGWKLWQGLLPEECTKSYPTEPWRCYYGYRLYPTLKTPLFVFQWLFDEAQMRVDNVGAPVTPQQWNYIHEMGGALRSSLDNVSAVFAPSCIGHGVLFKRDWVNIKIDDISLPSALRCWEHSTRSRRHDKLKRSTEPSTAVSHPEHANNQRHQRHRQRLQRQKHNNVAQSGGQQRKHNHLSKEEREERKRLRQEQRQRRKQRRRQQQQKKANGGQEHRNKKDNSPKSSNGNDQRKQRRRQQLTAEERQEQRKRRRKAQQQQMKMQREQPAAGVFLEASAPQKTRSSNNASAGTKSKKRHRVPRVPEKCGLRLLERCSWPQCNHSCPTLTNPMTGEEMRFLELLTAFGLDIEAVAAALGVDMHTLNNMERTELVNMLTQQAN

Target ORF information:

RefSeq Version	NM_168643.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster notum (Notum), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168643.3

ORF Insert Sequence:

ATGGCAGTCG AACAAATTGA TAAAATGGCA GCTAAAGCCG GAGAAGCTAC AAATAAATGG 
ATTAAGCCAC AGCAGCCGCT GCTAACGCTT CTGCTGCTCT TAGCCACGTT CAGTCAGCTG 
CCAGCCGTTT GCAGCAGCAG CATCTTGGAT GCGGCGAGTC TGCAGGAGAA GGATCCATTG 
AGGGACACCA GCATGAACAT GATCCAGCGC AACTACATGG TGATGCACTC GGCCAGCGGC 
TCCGGTGACC ACAGCCGCTC CCTGAAACGC GCCAATCTGG CCAACACGAG CATCACCTGC 
AACGATGGCA GTCATGCCGG TTTCTATCTG CGCAAGCATC CCAGCTCCAA GAAGTGGATC 
GTTCTACTCG AAGGTGGCTG GCACTGTTTC GATGTGCGCT CGTGCCGCTC CAGATGGATG 
CGACTGCGCC ACCTAATGAC CTCCTCCCAG TGGCCAGAGA CGAGAGATGT TGGCGGCATT 
CTATCGCCAC ATCCCGAGGA GAATCCCTAC TGGCACAATG CCAACCATGT GCTCATCCCG 
TACTGCAGCA GTGATTCGTG GTCGGGAACA CGAACGGAGC CGGATACCAG CGATCGGGAG 
AACAGTTGGC GGTTCATGGG TGCCCTGATT CTGCGCCAGG TGATCGCCGA GCTGATTCCC 
GTGGGATTGG GCCGAGTGCC GGGTGGCGAA CTGATGCTCG TTGGCTCATC GGCTGGTGGC 
ATGGGTGTAA TGCTCAATTT GGATCGCATT CGAGACTTTC TGGTCAACGA GAAAAAGCTA 
CAGATCACAG TGAGGGGAGT GAGTGACTCG GGTTGGTTCC TGGACAGAGA GCCATACACG 
CCGGCGGCCG TGGCCTCCAA TGAGGCAGTG CGTCAGGGTT GGAAGTTGTG GCAGGGCTTG 
CTGCCCGAGG AGTGCACCAA AAGTTATCCC ACGGAGCCAT GGAGATGTTA CTATGGATAC 
CGACTATATC CCACTTTGAA AACTCCGCTT TTCGTTTTCC AATGGCTTTT CGACGAGGCC 
CAAATGCGAG TGGATAATGT TGGAGCACCC GTGACGCCTC AGCAATGGAA CTACATCCAC 
GAAATGGGCG GCGCCCTGAG ATCCTCGCTG GACAATGTGA GCGCAGTGTT TGCACCCAGT 
TGCATAGGTC ATGGGGTGCT CTTCAAACGG GATTGGGTCA ATATCAAGAT CGACGATATA 
TCGCTGCCCT CAGCGCTGCG CTGTTGGGAG CACTCAACGC GCAGTAGGCG TCATGACAAG 
CTCAAGCGTT CCACGGAACC ATCGACGGCG GTCTCGCATC CAGAACATGC CAATAACCAA 
AGACACCAGC GACACCGTCA GCGGCTGCAG CGACAGAAGC ATAACAATGT GGCCCAATCT 
GGAGGGCAGC AGCGCAAGCA CAATCACCTG AGCAAGGAGG AGCGAGAGGA GCGAAAACGA 
TTGCGCCAGG AGCAGCGACA GAGGCGGAAG CAACGTCGCC GCCAGCAGCA GCAAAAGAAG 
GCCAATGGCG GGCAGGAGCA TCGCAATAAG AAGGACAACA GCCCCAAGTC GAGCAATGGC 
AATGATCAGC GGAAACAGCG ACGGCGCCAG CAATTGACAG CCGAGGAAAG GCAGGAGCAG 
CGCAAGCGAC GCAGGAAGGC TCAGCAGCAG CAGATGAAAA TGCAGCGGGA ACAGCCGGCG 
GCGGGTGTTT TCCTGGAAGC GAGTGCGCCC CAGAAGACAC GCTCCTCCAA CAACGCCTCC 
GCGGGCACAA AGTCCAAGAA GCGACATCGC GTTCCGCGAG TGCCAGAGAA ATGCGGCCTC 
CGTCTGCTGG AACGCTGCAG TTGGCCGCAA TGCAATCACT CGTGTCCCAC GCTCACCAAT 
CCCATGACCG GCGAGGAGAT GCGATTCCTC GAGCTGCTCA CCGCCTTTGG CTTGGACATC 
GAGGCGGTGG CCGCCGCTCT GGGAGTCGAT ATGCACACGC TCAACAACAT GGAGCGCACC 
GAGTTGGTCA ACATGCTCAC CCAGCAGGCT AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Notum cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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