Lrrk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lrrk
Entrez Gene ID	42447
Full Name	Leucine-rich repeat kinase
Synonyms	CG5483,CT17358,Dmel\CG5483,LRRK,LRRK2,dLRRK,lrrk
General protein information	Preferred Names Leucine-rich repeat kinase Names CG5483 gene product from transcript CG5483-RD CG5483-PB CG5483-PC CG5483-PD LRRK Lrrk-PB Lrrk-PC Lrrk-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 92F3-92F4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275842.1	NP_001262771.1	Leucine-rich repeat kinase, isoform C
NM_001275843.1	NP_001262772.1	Leucine-rich repeat kinase, isoform D
NM_001104377.2	NP_001097847.1	Leucine-rich repeat kinase, isoform B

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000810	XP_311349.5
Drosophila melanogaster (fruit fly)	Lrrk	NP_001097847.1
Caenorhabditis elegans (roundworm)	lrk-1	NP_492839.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our results suggest LRRK2 may be involved in a wide variety of cellular processes and the results from this screen provide an important genetic resource for further evaluation of LRRK2 function
Title: LRRK2(I2020T) functional genetic interactors that modify eye degeneration and dopaminergic cell loss in Drosophila.

Data support the view that LRRK2/Lrrk is a gene/protein involved in oxidative stress-signaling leading to neurodegenerative process and loss of DAergic neurons and that reduced/suppressed LRRK2 expression might delay or prevent motor symptoms and/or frank Parkinsonism in individuals at risk to suffer autosomal dominant Parkinsonism.
Title: Knockdown transgenic Lrrk Drosophila resists paraquat-induced locomotor impairment and neurodegeneration: A therapeutic strategy for Parkinson's disease.

EndophilinA-induced macroautophagy is activated when the kinase LRRK2 phosphorylates the EndophilinA-BAR domain and is blocked in animals where EndophilinA cannot be phosphorylated. Functionally, deregulation of the EndophilinA phosphorylation state accelerates activity-induced neurodegeneration.
Title: A LRRK2-Dependent EndophilinA Phosphoswitch Is Critical for Macroautophagy at Presynaptic Terminals.

Our findings demonstrated that lovastatin restored LRRK2-G2019S neurite degeneration by augmenting Akt/NRF2 pathway and inhibiting downstream GSK3beta activity, which decreased phospho-tau levels. We suggested that lovastatin is a potential disease-modifying agent for LRRK2-G2019S parkinsonism.
Title: Lovastatin protects neurite degeneration in LRRK2-G2019S parkinsonism through activating the Akt/Nrf pathway and inhibiting GSK3? activity.

The study reveals a pathogenic pathway for LRRK2 mutations causing dendrite degeneration.
Title: Lrrk regulates the dynamic profile of dendritic Golgi outposts through the golgin Lava lamp.

The following Lrrk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lrrk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05497	NM_001104377.2 Latest version!	Drosophila melanogaster Leucine-rich repeat kinase (Lrrk), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OFa07816	NM_001275843.1 Latest version!	Drosophila melanogaster Leucine-rich repeat kinase (Lrrk), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OFa07817	NM_001275842.1 Latest version!	Drosophila melanogaster Leucine-rich repeat kinase (Lrrk), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lrrk ( NM_001104377.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097847.1 Drosophila melanogaster Leucine-rich repeat kinase (Lrrk), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa05497
Clone ID Related Accession (Same CDS sequence)	NM_001104377.2
Accession Version	NM_001104377.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Leucine-rich repeat kinase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001104377.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAACATC CCAAAACTGG AACTGAAACA GCTCTGGAAG CCTGTGACTA CTTCGTGGAT 
GAGGTCATTG AGGCATCCTC AATACGGGAT GCCCGCGAGG AAGTGCGTCA GATCAAGCAC 
GGGGAGCTGC GAACGGCAGT GATCTCTGGC GATGAGCGGA CTGTGCGGGT GCTGCTGGCA 
GCTCTTGGAA CTGAGCGTCA GATTATAGTC AATATGGCTC CTTCAGGAGC AAACACCCTT 
CTATTCCTAG CCTGCCAGTC TGGTTACGAG AGCATTACAC AGCGATTGCT GGATGCTGGA 
GCGGATGGTC GCTCCCATGC CGTGACCAAG TACTCACCCT TGTACGCCGC CGTCCATAGC 
GGTCACTTGG GCATTGCCCG ACTGATGCTA GACCATTTTC CAGAACTGAT CCAGCAGCCG 
ACTGTAGAGC GCTGGCTGCC GCTGCACGCC GCCTGCATCA ATGGACACAT CAAGCTGCTG 
GAACTCCTTA TCAGCTACAG TTATCCCGAC TACCTCTACC AGACATATCG CGACGAGGAG 
GGCCAGTGGG AATGGCGGCT CCCCTTCGAC GCTAACGCTC ATGATGTGAC GGGTCAGACG 
AGTCTGTATA TTGCCAGCAT CCTAGGAAAC AAGCAGCTGG TTGGTGTGCT TCTAAAGTGG 
CAGCTCCATT GCCGGCGTAC GTTGGGCGAT TCCGCCAGCT CGGTGAGCAC TCCTATTACG 
CCCACCAGGA AACGTATTTC ATTTGGCATT CAGGCTATCA TGTCCAAACT GCACATATCT 
GGAGAGTCAG AAGGACCCGA CGACCTAGCT TCACAAGAGT CAACCGAGTG CCAACGGTGT 
CCCATTAACG TGAATCTGCT ATGTGGAGCG GCGAGAGAAA CAGCTCTGCT TGCGGCCGTT 
CGAGGCGGCC ACCTAGACGT GGTGCAGTCT CTGCTACAGC ACGGCGCAAA TCCGAATATT 
GTAGCCAAGC CAGTTGAGGA TCACAACGAC CCGAAATGTT GCGAGGAAAT ATATGGGCTC 
AGTAATGTCC CCATTGCAGA GGCCTGCAAG CAAAGGTCGC TGGCTATGTT GGATCTCTTG 
CTTAAGCATG GAGCCCGCGA CGATAATGGC ACGGCCATTG GAATGGCTAT TACATGCGGC 
GACGAGGCCA TCCTGAGTCG TCTTTTGGCC CGACGAGTCC ATCCGGACTC AGACTACAAG 
ATCAACAAGA AGGGTCTTCC CACACCAGTG GAGGTGAACG TGTTTTTGCC GTCCACCAGC 
AACATATCTT ACAGTGCTAT GTTCCCCAAC AATCCAACCA TTATTGACTG GCACAGCATG 
GGCTCCAGTG TCCAGTTGTC TGTTGTAAGG GTTCCCTGGA TGGTCAGCGG TGTGTTGCTG 
CTGAATCCTA AGCTACAGTC GCATCCGCGA CTTAATGAGG TTGCTCTGAC AGCTATCACG 
CGAATAGATT TCTCGCACAA CGTTCTCACG TCAATTCCAC AGGAGCTTTT CCATTTGGTT 
AGCCTAAGGT ATTTAAATGT GGCGCAAAAC AAGATAACCG ATCTACCTGC ACCAATAGGC 
CAGACATATG GGTGTCCGGT ACTGGATGAA CTCTTTCTAC AAGACAACCA GTTAACTACG 
CTGCCAGCCG CCATCTTTCA CCTGCCCGCC CTATCTATAT TGGATGTTTC AAATAACAAA 
CTGCAACAGC TTCCCTTCGA CCTGTGGCGT GCACCCAAGT TGCGCGAACT TAATGTGGCC 
TTCAACCTAT TGCGGGATCT TCCTGTGCCG CCAATGCAAA CTAGCAGTTC GCTGCTGAGC 
CTAGACAAAT TGAATCTGCA ATCTTTTGAG GAGCCGCCAT CAAACAAACC GCGAAATGTG 
ACTCAGCAAC GTCTCACCCA TCGCAATCTA TGGTCGGCTA CTTTGGACAT AACCGATAAT 
GACATGAAGT GGCAGCATGA ACAAGATTTA GGCGATGGAA AGACAGCTGG TGTGGGCTCC 
TCCCAGCTAA GTAGCTTAAA TATAGCAAAT AACCTCTTCA CCAGTATCCC CGCTGCTTTG 
CCCTGTCTGG CTGTAAACCT AACACGGCTG AATATGTCAT ACAACAGTCT GCGTTCTATG 
GGACATGTAA CCAGTTACCC GGCAACACTT AAGCAGTTGG ATCTAAGTCA CAACGAGATA 
TCCTGCTGGC CAAGTTTACC GCGAATTACT GAATCGGATC CGCATCTACT GTGCTACAGT 
TGTGTCCAGC TACCAGAGGG TCGAGATGAC GACTACAAGA CGGCCTCCTC AAAGGGCAGT 
AGCTCGTCTG CCACATCGTT TCGCGCTTCG GTGTTAAAAA GTGTTTGTCG GCACAGGCGT 
CATCTGCGCC TGGAGGCTCT ACGCACATTG ATCTTGGCTG ACAATCTGCT TACGCGCATC 
CAACTATCAA CTGATGACGC CACAACCCTT TTCAATGAAA GTGAGGACGC CGATTGGAGT 
GTGGTAGGCG TGAACCGGTC TAAGGTGATA TTTCCCAACC TGTCTATGCT GGATATGACC 
AACAATTGCC TGAAGGAGAT TCCCGCCTCG TTGCACGAAC TGAGCAGTCT GTCAGTGCTG 
AATATTAGTG GCAACGTCAA CATCACGGAA CTGCCGCCGC ACTTGGGACT GCTGTCGAGA 
CTTTGGAATC TTAATACGCG CGGTTGCCTA CTGCAGGAGC CACTGCGCTC TATGATCGAG 
AGCAAGAAGC ACAAGACGAT GGACATTGTT GGATATCTTA AATCTATTTA CGAAGATGCT 
CAGCCCTACG CACGGATGAA ACTGATGGTG GTTGGTGTGG CTGGAATTGG CAAGAGCACT 
CTGCTGGACT TACTGCGCCA GGGGGCGGGC TCAGGGTCCA GCTCCAGCTC TCATCGATCG 
CGCGCCAGCG AAAATCATTG GGCCAAGCGA ATGGGACACG CACGCAGCAC ATCTCGATCT 
CATCGCCATT CGTCGGCTTC TAGCGCAAAC ATTTCTACAG TAGGAGTGGA CATTGGAACA 
TGGATATGTG AGAAACGAAA GCGAGCACCC GGCTCCCACG GCCCAGTGGT GTTTCGTACT 
TGGGATTTTG GTGGTCAGAA GGAGTACTAC GCCACACATC AGTACTTTTT GTCCAAACGG 
AGTCTGTATT TGGTGCTCTG GCGCATTAGC GACGGTCACA AGGGTCTGGC GGAGTTGCTG 
CAGTGGCTTG GCAACATTCA AGCGAGGGCT CCCAACTCCC CCGTAATCAT TGTGGGCACA 
CACTTTGATG CCGTTGGTGA ATCCATCTCG CCCCAAAAGG CAGAGCAACT GCAGCAATTG 
ATTCGGGAGA AGTTTATCGC CATTCCCGAT GCGGAAAAAA TTGGGCTGCC ACGGGTGATT 
GATTCCATCG AAATAAGTTG CCGGACCCTA CACAATATCC ACTTATTGGC CAACATTATT 
TACGACACCG CCATGCAACT GCGATCCCCT GGTTCCAAGG AGCCCATGCT ACTGCAAAAG 
ATCCCCGCCA GCTACATTGC CTTGGAGGAT ATTGTGAATG TGATTGCATG CAATCTGCGC 
GCTGCTGGGC GAGATCCCGT CCTGGATGGT GAACAATATA AGCGCTTGGT CACCGAACAG 
ATGAGATTGC ACAACTACAA GAGCTTCCGG GATGCCGCTG AGCTGCAGCA GGCCACTACA 
TGGTGCCACG AAAATGGAGT TTTATTGCAC TATGATGATG CTACGCTAAG GGACTACTAC 
TTTCTCGATC CACAGTGGCT TTGCGACATG CTGGCCCATG TGGTCACCGT GCGGGAGATC 
AACCCCTTTG CTCCTACGGG CGTGATGAAG CTGGATGATC TCCAGATGTT GTTCCGAAGT 
GTGCAGGTTC AAGGAAATGG AAACAGAAGC TACATTGTCA GTCTACTTAA CAAGTTTGAG 
GTTGCTTTAA CGTGGGATTC GAGAACTCTA CTCATACCCT CCTTACTGCC TTCGCAGGAG 
GCAGCTACAC CCAATTCAGG AAGCACGGTT AAGCTATCGC AACGATCTCG CGGCCGTAGT 
TTGGGTTGCT CTGTATCGCA AGAAGTAAAT CTAAATAATT TGATATACGA ACAGAGGTCA 
GCTCCTTCTT CATCATCTTC CTCTGCATCG GTTAGCCAAG GCTTGCGGCG CATCCTATTG 
ATGACTTATT TCCCGTCCGG GTTTTGGTCA AGATTAATCA CCCGGATTTT AGCTGATGAA 
CAAATAATCG AAGCAATTCG TGGCGTTTAT ATGGCTTCAC AAGATTACGC GGACTTTGAT 
CTACGCACAT CGTTGGAGCA GGACACCCAA TGGAATCTTT GGCAGACAGG CTTAGCACTG 
TACTATGGAC CTATCTTAAT ATTCAAGATC TGGGAAGTAC CCTTTCAGAA GACAGAGCGA 
ACGCAACCTT TCCGCACTGA TGGCAACCGA TTTAAGCTGA AACAAGATGG TATCTGGAGC 
GATGTAAATC TCAGCTCCTC TAGCATTTTG GAAGTTTACT TTCCACTCTA TGAAGTGAAC 
ATATCGCAAG AAGTGGATGA CAATGAACGT CAATTGCTGG CCGAGATTCG TCCGCACATG 
TCTCAGGTGG CCAAGCTTTT GGCCCTAACA GTGGATCATA TTGACTTGCT TTTGGAGGAT 
TGGTATCCTT CATTGGGAAC GCGGTTTGTG CACACTTCCG AGGGTCGATT CCTAATCACT 
CGATTGGTGT TGTGTCCGCG ATGTCTTTGG AAGTTGCAGC TGCAGCAGAA CAACGAACCG 
TCTGATCGGG AAGTGCCTCC AGTGGGTTGC AATCGACCAA GTCGAAGTAG TAGGCGTGGA 
GCGGGAGCAT ACTTTCTCCA CGGTGTAGGT GATCCCGGAG AGGATGGTGC TTTAAACGTG 
TTCTCGGCCT ATTTAAACGC CACAGCAAGG AGAGAACGCC GATCGGAGGA TTCTTTGGGT 
GCTGGATCCG ACGCGGACTC GGGCGTGGGT CCCGACTCAG CCGGTTCTTC GCGCAACACT 
TCAGTTGATG GTCATCCCGG ATATCACTTG CCTGATAACT CAAATGTGTG TTATGCTTGG 
ATGATTGAGG AGTGCATTTT GTCCGTTTAT AATCAAAGCA AGATTAGTTG CCCTGTTCAT 
CTTGAACAGT CTATGGCTCA GCTTGCGCCA GACGTAATCT TTGCCGATAT TCCTGATAAG 
CACACTATTC CAAGCGAGTG CATCATCAAG GGATCGTTGC TTGGTCGAGG TGCTTTTGGG 
TTCGTTTTTA AAGCAAATTG CAAGGTAAGG GGCGCCAGAT CCTTTAAGCC GGTGGCAATG 
AAAATGCTTC AGCCAGTGCC TCCCGGAGCT CGTGCAAAAG AGAGCGCTTT GATGGCTTTC 
AAGGTAGCTG TAGGCAAATG GGACCGGGAT CCACTGCAGC ACTCTTGCAA GGCCTACTGC 
ACTGCGCGTC AGGAATTGGC AGTACTTCTA ACTCTCAAGC ATCCAAACAT TGTACCCTTG 
GTCGGGATCT GCATTAAACC ATTAGCTCTG GTTCTGGAAC TAGCTCCTCT AGGCGGTTTG 
GACGCTTTGC TCCGGCATTA CCGACGCAGC GGAGCCCACA TGGGACCGCA TACTTTCCAG 
ACCCTTGTCC TGCAGGCGGC TCGAGCAATC GAGTATTTGC ATCGCAGAAG AATCATCTAC 
CGCGATCTAA AGTCTGAAAA TGTCTTGGTT TGGGAGCTTC CACAACCACA CACGGAAGAC 
AGTCCCCGCA ACCTTGTGCA CATAAAGATT GCCGACTACG GAATTAGCCG GCAAACTGCT 
CCAAGTGGAG CTAAAGGATT TGGTGGCACC GAGGGTTTTA TGGCTCCGGA GATCATACGC 
TACAATGGTG AGGAGGAATA TACTGAAAAG GTGGACTGCT TCTCATTTGG AATGTTCATA 
TATGAGAATA TCAGTTTACG ACAACCTTTC GAAGGCCACG AGTCTATTAA AGAGTGCATC 
TTGGAGGGTA GTCGCCCGGC TTTGACTCAA AGGGAAACCC AGTTTCCCAC CTGCTGTTTG 
GATCTTATGG TCTTGTGTTG GCACGAACAG CCTCGTCGCA GACCGACGGC AAGTCAGATT 
GTTTCCATAC TAAGTGCACC GGAGTGCATC CACCTCCTGG ATGTCGTTGC CATGCCGCAT 
AGCGAGAAGA TTGTCTGTGG GGTTTTTCAG TCGCTTGTTG GTATGGGCGA TGATGAACGA 
TGTGGTCTTG AACTGTGGCT ACCCTCCTTC GGCTCTCGCA TTGACATTCT AGACTGCTCA 
CCCTCGGGCA GCCTACTGCA GTGCAACAGC ATCAGTTGTT CTCCGCAGCC ACAGGTTGCT 
CCGCCCAAGA CACCCGAAAA CGGTGCAAAT TCACGTGCCC GCTCTGCCCA ACGTTTGCCC 
AAGATGAACA TGTTGTGCTG CTGCCTGGTG GGTGAAGCCA TCTGGATGGG CGACGTTTCC 
GGCAATCTGC ACGCCTATAG CACATCCACC TATGCCCACC TGTTTTCCTA CATGCTCGAT 
CCCAACATTA AGTCAGCTGT GATCAGTCTA GTCTACATGG AGAAAATAGC TCGCGTGGCT 
GTCGGAACGC ATAACGGTCG GGTGTTCCTT GTTGACGCCA CGCAGATGCC CAGCAATTGC 
GCATTTGCCG AGGGCTCTTT TGTGCTTACA GAGATCTGTT CTGGTTTTGT GTTGCACGCC 
GCTTGTTCCG TTGTTGTGGA TGGAATCTAT GAATTGTGGT GCGGCGAAAT TGCAGGAAAG 
ATAAATGTTT TTCCGTTGAA CGAGAATGGA GTTTCCGGAC ATCAGGCTTT ATGCCACAGC 
GAGGAACCCA ATCTCATCGA GGACGTCAAG GTGGCTCGAA TGTGCAGTAA TGAAAGCCAT 
GTGTTCAGTT GTCTATATCC CGGTTGTATG GTGTACCAGT GGGATGTTAT CTCCAAGCGG 
ATAGAAAATA AGCTGGACTG CTCAAAGCTG CTGCCCTGCT CCGAGTCTCT GCAAAGTATT 
GCGATTGATG AGCATGTGAA CCTTATAAAA TGCCAGATTT CGGCACTGGC GGCTCACAAT 
TCAGAACTGT ATATTGGGAC GACCTGGGGC TGCTTAATTG TGGCCGAACT GCACACCCTG 
CGCCCCATTA GTGTTTTCCG ACCCTATGAA AATGAGATCA AATCTATAAT AACCCTTTCT 
AAAGACAATG TTCCGCTGAT CGCCACGATC GGAAGACGAT ATCGCTCTCT GATCTCTCGA 
TATGTGGACT CCGCAGAGTC ATCCACCAAG AGCTCTGCCG TCAGCACACC CACCCATGGT 
GCAGCCAAAT CCGTGCCACC GGCGGATGTG GATAATCACA TTCATTGCCT GCTGTGGCGC 
GCCAAGCACT GGACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097847.1
CDS	90..7427
Translation	MEHPKTGTETALEACDYFVDEVIEASSIRDAREEVRQIKHGELRTAVISGDERTVRVLLAALGTERQIIVNMAPSGANTLLFLACQSGYESITQRLLDAGADGRSHAVTKYSPLYAAVHSGHLGIARLMLDHFPELIQQPTVERWLPLHAACINGHIKLLELLISYSYPDYLYQTYRDEEGQWEWRLPFDANAHDVTGQTSLYIASILGNKQLVGVLLKWQLHCRRTLGDSASSVSTPITPTRKRISFGIQAIMSKLHISGESEGPDDLASQESTECQRCPINVNLLCGAARETALLAAVRGGHLDVVQSLLQHGANPNIVAKPVEDHNDPKCCEEIYGLSNVPIAEACKQRSLAMLDLLLKHGARDDNGTAIGMAITCGDEAILSRLLARRVHPDSDYKINKKGLPTPVEVNVFLPSTSNISYSAMFPNNPTIIDWHSMGSSVQLSVVRVPWMVSGVLLLNPKLQSHPRLNEVALTAITRIDFSHNVLTSIPQELFHLVSLRYLNVAQNKITDLPAPIGQTYGCPVLDELFLQDNQLTTLPAAIFHLPALSILDVSNNKLQQLPFDLWRAPKLRELNVAFNLLRDLPVPPMQTSSSLLSLDKLNLQSFEEPPSNKPRNVTQQRLTHRNLWSATLDITDNDMKWQHEQDLGDGKTAGVGSSQLSSLNIANNLFTSIPAALPCLAVNLTRLNMSYNSLRSMGHVTSYPATLKQLDLSHNEISCWPSLPRITESDPHLLCYSCVQLPEGRDDDYKTASSKGSSSSATSFRASVLKSVCRHRRHLRLEALRTLILADNLLTRIQLSTDDATTLFNESEDADWSVVGVNRSKVIFPNLSMLDMTNNCLKEIPASLHELSSLSVLNISGNVNITELPPHLGLLSRLWNLNTRGCLLQEPLRSMIESKKHKTMDIVGYLKSIYEDAQPYARMKLMVVGVAGIGKSTLLDLLRQGAGSGSSSSSHRSRASENHWAKRMGHARSTSRSHRHSSASSANISTVGVDIGTWICEKRKRAPGSHGPVVFRTWDFGGQKEYYATHQYFLSKRSLYLVLWRISDGHKGLAELLQWLGNIQARAPNSPVIIVGTHFDAVGESISPQKAEQLQQLIREKFIAIPDAEKIGLPRVIDSIEISCRTLHNIHLLANIIYDTAMQLRSPGSKEPMLLQKIPASYIALEDIVNVIACNLRAAGRDPVLDGEQYKRLVTEQMRLHNYKSFRDAAELQQATTWCHENGVLLHYDDATLRDYYFLDPQWLCDMLAHVVTVREINPFAPTGVMKLDDLQMLFRSVQVQGNGNRSYIVSLLNKFEVALTWDSRTLLIPSLLPSQEAATPNSGSTVKLSQRSRGRSLGCSVSQEVNLNNLIYEQRSAPSSSSSSASVSQGLRRILLMTYFPSGFWSRLITRILADEQIIEAIRGVYMASQDYADFDLRTSLEQDTQWNLWQTGLALYYGPILIFKIWEVPFQKTERTQPFRTDGNRFKLKQDGIWSDVNLSSSSILEVYFPLYEVNISQEVDDNERQLLAEIRPHMSQVAKLLALTVDHIDLLLEDWYPSLGTRFVHTSEGRFLITRLVLCPRCLWKLQLQQNNEPSDREVPPVGCNRPSRSSRRGAGAYFLHGVGDPGEDGALNVFSAYLNATARRERRSEDSLGAGSDADSGVGPDSAGSSRNTSVDGHPGYHLPDNSNVCYAWMIEECILSVYNQSKISCPVHLEQSMAQLAPDVIFADIPDKHTIPSECIIKGSLLGRGAFGFVFKANCKVRGARSFKPVAMKMLQPVPPGARAKESALMAFKVAVGKWDRDPLQHSCKAYCTARQELAVLLTLKHPNIVPLVGICIKPLALVLELAPLGGLDALLRHYRRSGAHMGPHTFQTLVLQAARAIEYLHRRRIIYRDLKSENVLVWELPQPHTEDSPRNLVHIKIADYGISRQTAPSGAKGFGGTEGFMAPEIIRYNGEEEYTEKVDCFSFGMFIYENISLRQPFEGHESIKECILEGSRPALTQRETQFPTCCLDLMVLCWHEQPRRRPTASQIVSILSAPECIHLLDVVAMPHSEKIVCGVFQSLVGMGDDERCGLELWLPSFGSRIDILDCSPSGSLLQCNSISCSPQPQVAPPKTPENGANSRARSAQRLPKMNMLCCCLVGEAIWMGDVSGNLHAYSTSTYAHLFSYMLDPNIKSAVISLVYMEKIARVAVGTHNGRVFLVDATQMPSNCAFAEGSFVLTEICSGFVLHAACSVVVDGIYELWCGEIAGKINVFPLNENGVSGHQALCHSEEPNLIEDVKVARMCSNESHVFSCLYPGCMVYQWDVISKRIENKLDCSKLLPCSESLQSIAIDEHVNLIKCQISALAAHNSELYIGTTWGCLIVAELHTLRPISVFRPYENEIKSIITLSKDNVPLIATIGRRYRSLISRYVDSAESSTKSSAVSTPTHGAAKSVPPADVDNHIHCLLWRAKHWT

Target ORF information:

RefSeq Version	NM_001104377.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Leucine-rich repeat kinase (Lrrk), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001104377.2

ORF Insert Sequence:

ATGGAACATC CCAAAACTGG AACTGAAACA GCTCTGGAAG CCTGTGACTA CTTCGTGGAT 
GAGGTCATTG AGGCATCCTC AATACGGGAT GCCCGCGAGG AAGTGCGTCA GATCAAGCAC 
GGGGAGCTGC GAACGGCAGT GATCTCTGGC GATGAGCGGA CTGTGCGGGT GCTGCTGGCA 
GCTCTTGGAA CTGAGCGTCA GATTATAGTC AATATGGCTC CTTCAGGAGC AAACACCCTT 
CTATTCCTAG CCTGCCAGTC TGGTTACGAG AGCATTACAC AGCGATTGCT GGATGCTGGA 
GCGGATGGTC GCTCCCATGC CGTGACCAAG TACTCACCCT TGTACGCCGC CGTCCATAGC 
GGTCACTTGG GCATTGCCCG ACTGATGCTA GACCATTTTC CAGAACTGAT CCAGCAGCCG 
ACTGTAGAGC GCTGGCTGCC GCTGCACGCC GCCTGCATCA ATGGACACAT CAAGCTGCTG 
GAACTCCTTA TCAGCTACAG TTATCCCGAC TACCTCTACC AGACATATCG CGACGAGGAG 
GGCCAGTGGG AATGGCGGCT CCCCTTCGAC GCTAACGCTC ATGATGTGAC GGGTCAGACG 
AGTCTGTATA TTGCCAGCAT CCTAGGAAAC AAGCAGCTGG TTGGTGTGCT TCTAAAGTGG 
CAGCTCCATT GCCGGCGTAC GTTGGGCGAT TCCGCCAGCT CGGTGAGCAC TCCTATTACG 
CCCACCAGGA AACGTATTTC ATTTGGCATT CAGGCTATCA TGTCCAAACT GCACATATCT 
GGAGAGTCAG AAGGACCCGA CGACCTAGCT TCACAAGAGT CAACCGAGTG CCAACGGTGT 
CCCATTAACG TGAATCTGCT ATGTGGAGCG GCGAGAGAAA CAGCTCTGCT TGCGGCCGTT 
CGAGGCGGCC ACCTAGACGT GGTGCAGTCT CTGCTACAGC ACGGCGCAAA TCCGAATATT 
GTAGCCAAGC CAGTTGAGGA TCACAACGAC CCGAAATGTT GCGAGGAAAT ATATGGGCTC 
AGTAATGTCC CCATTGCAGA GGCCTGCAAG CAAAGGTCGC TGGCTATGTT GGATCTCTTG 
CTTAAGCATG GAGCCCGCGA CGATAATGGC ACGGCCATTG GAATGGCTAT TACATGCGGC 
GACGAGGCCA TCCTGAGTCG TCTTTTGGCC CGACGAGTCC ATCCGGACTC AGACTACAAG 
ATCAACAAGA AGGGTCTTCC CACACCAGTG GAGGTGAACG TGTTTTTGCC GTCCACCAGC 
AACATATCTT ACAGTGCTAT GTTCCCCAAC AATCCAACCA TTATTGACTG GCACAGCATG 
GGCTCCAGTG TCCAGTTGTC TGTTGTAAGG GTTCCCTGGA TGGTCAGCGG TGTGTTGCTG 
CTGAATCCTA AGCTACAGTC GCATCCGCGA CTTAATGAGG TTGCTCTGAC AGCTATCACG 
CGAATAGATT TCTCGCACAA CGTTCTCACG TCAATTCCAC AGGAGCTTTT CCATTTGGTT 
AGCCTAAGGT ATTTAAATGT GGCGCAAAAC AAGATAACCG ATCTACCTGC ACCAATAGGC 
CAGACATATG GGTGTCCGGT ACTGGATGAA CTCTTTCTAC AAGACAACCA GTTAACTACG 
CTGCCAGCCG CCATCTTTCA CCTGCCCGCC CTATCTATAT TGGATGTTTC AAATAACAAA 
CTGCAACAGC TTCCCTTCGA CCTGTGGCGT GCACCCAAGT TGCGCGAACT TAATGTGGCC 
TTCAACCTAT TGCGGGATCT TCCTGTGCCG CCAATGCAAA CTAGCAGTTC GCTGCTGAGC 
CTAGACAAAT TGAATCTGCA ATCTTTTGAG GAGCCGCCAT CAAACAAACC GCGAAATGTG 
ACTCAGCAAC GTCTCACCCA TCGCAATCTA TGGTCGGCTA CTTTGGACAT AACCGATAAT 
GACATGAAGT GGCAGCATGA ACAAGATTTA GGCGATGGAA AGACAGCTGG TGTGGGCTCC 
TCCCAGCTAA GTAGCTTAAA TATAGCAAAT AACCTCTTCA CCAGTATCCC CGCTGCTTTG 
CCCTGTCTGG CTGTAAACCT AACACGGCTG AATATGTCAT ACAACAGTCT GCGTTCTATG 
GGACATGTAA CCAGTTACCC GGCAACACTT AAGCAGTTGG ATCTAAGTCA CAACGAGATA 
TCCTGCTGGC CAAGTTTACC GCGAATTACT GAATCGGATC CGCATCTACT GTGCTACAGT 
TGTGTCCAGC TACCAGAGGG TCGAGATGAC GACTACAAGA CGGCCTCCTC AAAGGGCAGT 
AGCTCGTCTG CCACATCGTT TCGCGCTTCG GTGTTAAAAA GTGTTTGTCG GCACAGGCGT 
CATCTGCGCC TGGAGGCTCT ACGCACATTG ATCTTGGCTG ACAATCTGCT TACGCGCATC 
CAACTATCAA CTGATGACGC CACAACCCTT TTCAATGAAA GTGAGGACGC CGATTGGAGT 
GTGGTAGGCG TGAACCGGTC TAAGGTGATA TTTCCCAACC TGTCTATGCT GGATATGACC 
AACAATTGCC TGAAGGAGAT TCCCGCCTCG TTGCACGAAC TGAGCAGTCT GTCAGTGCTG 
AATATTAGTG GCAACGTCAA CATCACGGAA CTGCCGCCGC ACTTGGGACT GCTGTCGAGA 
CTTTGGAATC TTAATACGCG CGGTTGCCTA CTGCAGGAGC CACTGCGCTC TATGATCGAG 
AGCAAGAAGC ACAAGACGAT GGACATTGTT GGATATCTTA AATCTATTTA CGAAGATGCT 
CAGCCCTACG CACGGATGAA ACTGATGGTG GTTGGTGTGG CTGGAATTGG CAAGAGCACT 
CTGCTGGACT TACTGCGCCA GGGGGCGGGC TCAGGGTCCA GCTCCAGCTC TCATCGATCG 
CGCGCCAGCG AAAATCATTG GGCCAAGCGA ATGGGACACG CACGCAGCAC ATCTCGATCT 
CATCGCCATT CGTCGGCTTC TAGCGCAAAC ATTTCTACAG TAGGAGTGGA CATTGGAACA 
TGGATATGTG AGAAACGAAA GCGAGCACCC GGCTCCCACG GCCCAGTGGT GTTTCGTACT 
TGGGATTTTG GTGGTCAGAA GGAGTACTAC GCCACACATC AGTACTTTTT GTCCAAACGG 
AGTCTGTATT TGGTGCTCTG GCGCATTAGC GACGGTCACA AGGGTCTGGC GGAGTTGCTG 
CAGTGGCTTG GCAACATTCA AGCGAGGGCT CCCAACTCCC CCGTAATCAT TGTGGGCACA 
CACTTTGATG CCGTTGGTGA ATCCATCTCG CCCCAAAAGG CAGAGCAACT GCAGCAATTG 
ATTCGGGAGA AGTTTATCGC CATTCCCGAT GCGGAAAAAA TTGGGCTGCC ACGGGTGATT 
GATTCCATCG AAATAAGTTG CCGGACCCTA CACAATATCC ACTTATTGGC CAACATTATT 
TACGACACCG CCATGCAACT GCGATCCCCT GGTTCCAAGG AGCCCATGCT ACTGCAAAAG 
ATCCCCGCCA GCTACATTGC CTTGGAGGAT ATTGTGAATG TGATTGCATG CAATCTGCGC 
GCTGCTGGGC GAGATCCCGT CCTGGATGGT GAACAATATA AGCGCTTGGT CACCGAACAG 
ATGAGATTGC ACAACTACAA GAGCTTCCGG GATGCCGCTG AGCTGCAGCA GGCCACTACA 
TGGTGCCACG AAAATGGAGT TTTATTGCAC TATGATGATG CTACGCTAAG GGACTACTAC 
TTTCTCGATC CACAGTGGCT TTGCGACATG CTGGCCCATG TGGTCACCGT GCGGGAGATC 
AACCCCTTTG CTCCTACGGG CGTGATGAAG CTGGATGATC TCCAGATGTT GTTCCGAAGT 
GTGCAGGTTC AAGGAAATGG AAACAGAAGC TACATTGTCA GTCTACTTAA CAAGTTTGAG 
GTTGCTTTAA CGTGGGATTC GAGAACTCTA CTCATACCCT CCTTACTGCC TTCGCAGGAG 
GCAGCTACAC CCAATTCAGG AAGCACGGTT AAGCTATCGC AACGATCTCG CGGCCGTAGT 
TTGGGTTGCT CTGTATCGCA AGAAGTAAAT CTAAATAATT TGATATACGA ACAGAGGTCA 
GCTCCTTCTT CATCATCTTC CTCTGCATCG GTTAGCCAAG GCTTGCGGCG CATCCTATTG 
ATGACTTATT TCCCGTCCGG GTTTTGGTCA AGATTAATCA CCCGGATTTT AGCTGATGAA 
CAAATAATCG AAGCAATTCG TGGCGTTTAT ATGGCTTCAC AAGATTACGC GGACTTTGAT 
CTACGCACAT CGTTGGAGCA GGACACCCAA TGGAATCTTT GGCAGACAGG CTTAGCACTG 
TACTATGGAC CTATCTTAAT ATTCAAGATC TGGGAAGTAC CCTTTCAGAA GACAGAGCGA 
ACGCAACCTT TCCGCACTGA TGGCAACCGA TTTAAGCTGA AACAAGATGG TATCTGGAGC 
GATGTAAATC TCAGCTCCTC TAGCATTTTG GAAGTTTACT TTCCACTCTA TGAAGTGAAC 
ATATCGCAAG AAGTGGATGA CAATGAACGT CAATTGCTGG CCGAGATTCG TCCGCACATG 
TCTCAGGTGG CCAAGCTTTT GGCCCTAACA GTGGATCATA TTGACTTGCT TTTGGAGGAT 
TGGTATCCTT CATTGGGAAC GCGGTTTGTG CACACTTCCG AGGGTCGATT CCTAATCACT 
CGATTGGTGT TGTGTCCGCG ATGTCTTTGG AAGTTGCAGC TGCAGCAGAA CAACGAACCG 
TCTGATCGGG AAGTGCCTCC AGTGGGTTGC AATCGACCAA GTCGAAGTAG TAGGCGTGGA 
GCGGGAGCAT ACTTTCTCCA CGGTGTAGGT GATCCCGGAG AGGATGGTGC TTTAAACGTG 
TTCTCGGCCT ATTTAAACGC CACAGCAAGG AGAGAACGCC GATCGGAGGA TTCTTTGGGT 
GCTGGATCCG ACGCGGACTC GGGCGTGGGT CCCGACTCAG CCGGTTCTTC GCGCAACACT 
TCAGTTGATG GTCATCCCGG ATATCACTTG CCTGATAACT CAAATGTGTG TTATGCTTGG 
ATGATTGAGG AGTGCATTTT GTCCGTTTAT AATCAAAGCA AGATTAGTTG CCCTGTTCAT 
CTTGAACAGT CTATGGCTCA GCTTGCGCCA GACGTAATCT TTGCCGATAT TCCTGATAAG 
CACACTATTC CAAGCGAGTG CATCATCAAG GGATCGTTGC TTGGTCGAGG TGCTTTTGGG 
TTCGTTTTTA AAGCAAATTG CAAGGTAAGG GGCGCCAGAT CCTTTAAGCC GGTGGCAATG 
AAAATGCTTC AGCCAGTGCC TCCCGGAGCT CGTGCAAAAG AGAGCGCTTT GATGGCTTTC 
AAGGTAGCTG TAGGCAAATG GGACCGGGAT CCACTGCAGC ACTCTTGCAA GGCCTACTGC 
ACTGCGCGTC AGGAATTGGC AGTACTTCTA ACTCTCAAGC ATCCAAACAT TGTACCCTTG 
GTCGGGATCT GCATTAAACC ATTAGCTCTG GTTCTGGAAC TAGCTCCTCT AGGCGGTTTG 
GACGCTTTGC TCCGGCATTA CCGACGCAGC GGAGCCCACA TGGGACCGCA TACTTTCCAG 
ACCCTTGTCC TGCAGGCGGC TCGAGCAATC GAGTATTTGC ATCGCAGAAG AATCATCTAC 
CGCGATCTAA AGTCTGAAAA TGTCTTGGTT TGGGAGCTTC CACAACCACA CACGGAAGAC 
AGTCCCCGCA ACCTTGTGCA CATAAAGATT GCCGACTACG GAATTAGCCG GCAAACTGCT 
CCAAGTGGAG CTAAAGGATT TGGTGGCACC GAGGGTTTTA TGGCTCCGGA GATCATACGC 
TACAATGGTG AGGAGGAATA TACTGAAAAG GTGGACTGCT TCTCATTTGG AATGTTCATA 
TATGAGAATA TCAGTTTACG ACAACCTTTC GAAGGCCACG AGTCTATTAA AGAGTGCATC 
TTGGAGGGTA GTCGCCCGGC TTTGACTCAA AGGGAAACCC AGTTTCCCAC CTGCTGTTTG 
GATCTTATGG TCTTGTGTTG GCACGAACAG CCTCGTCGCA GACCGACGGC AAGTCAGATT 
GTTTCCATAC TAAGTGCACC GGAGTGCATC CACCTCCTGG ATGTCGTTGC CATGCCGCAT 
AGCGAGAAGA TTGTCTGTGG GGTTTTTCAG TCGCTTGTTG GTATGGGCGA TGATGAACGA 
TGTGGTCTTG AACTGTGGCT ACCCTCCTTC GGCTCTCGCA TTGACATTCT AGACTGCTCA 
CCCTCGGGCA GCCTACTGCA GTGCAACAGC ATCAGTTGTT CTCCGCAGCC ACAGGTTGCT 
CCGCCCAAGA CACCCGAAAA CGGTGCAAAT TCACGTGCCC GCTCTGCCCA ACGTTTGCCC 
AAGATGAACA TGTTGTGCTG CTGCCTGGTG GGTGAAGCCA TCTGGATGGG CGACGTTTCC 
GGCAATCTGC ACGCCTATAG CACATCCACC TATGCCCACC TGTTTTCCTA CATGCTCGAT 
CCCAACATTA AGTCAGCTGT GATCAGTCTA GTCTACATGG AGAAAATAGC TCGCGTGGCT 
GTCGGAACGC ATAACGGTCG GGTGTTCCTT GTTGACGCCA CGCAGATGCC CAGCAATTGC 
GCATTTGCCG AGGGCTCTTT TGTGCTTACA GAGATCTGTT CTGGTTTTGT GTTGCACGCC 
GCTTGTTCCG TTGTTGTGGA TGGAATCTAT GAATTGTGGT GCGGCGAAAT TGCAGGAAAG 
ATAAATGTTT TTCCGTTGAA CGAGAATGGA GTTTCCGGAC ATCAGGCTTT ATGCCACAGC 
GAGGAACCCA ATCTCATCGA GGACGTCAAG GTGGCTCGAA TGTGCAGTAA TGAAAGCCAT 
GTGTTCAGTT GTCTATATCC CGGTTGTATG GTGTACCAGT GGGATGTTAT CTCCAAGCGG 
ATAGAAAATA AGCTGGACTG CTCAAAGCTG CTGCCCTGCT CCGAGTCTCT GCAAAGTATT 
GCGATTGATG AGCATGTGAA CCTTATAAAA TGCCAGATTT CGGCACTGGC GGCTCACAAT 
TCAGAACTGT ATATTGGGAC GACCTGGGGC TGCTTAATTG TGGCCGAACT GCACACCCTG 
CGCCCCATTA GTGTTTTCCG ACCCTATGAA AATGAGATCA AATCTATAAT AACCCTTTCT 
AAAGACAATG TTCCGCTGAT CGCCACGATC GGAAGACGAT ATCGCTCTCT GATCTCTCGA 
TATGTGGACT CCGCAGAGTC ATCCACCAAG AGCTCTGCCG TCAGCACACC CACCCATGGT 
GCAGCCAAAT CCGTGCCACC GGCGGATGTG GATAATCACA TTCATTGCCT GCTGTGGCGC 
GCCAAGCACT GGACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lrrk ( NM_001275843.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262772.1 Drosophila melanogaster Leucine-rich repeat kinase (Lrrk), transcript variant D, mRNA.

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CloneID	OFa07816
Clone ID Related Accession (Same CDS sequence)	NM_001275843.1
Accession Version	NM_001275843.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Leucine-rich repeat kinase, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGACT CCGACGAAGC AAGGGCACAG GGTGACATTG GTGCAATGGA AGTGGGCGCG 
CAAAAACTTT TTTCAAACAG CCACCCCCCA CAGAAGCAAC TGGTGCAACC ACTGGACCAT 
CCGACACCCA CGAAGAGACA AATCAAAACA AATGCCACGC ACTCACCCAA TGCCACATTC 
TTGAACAAGT GCAACAGTTG CCGCGCTTCT TGGGCTCCGA CTTGCGCAGT GCGACATAAA 
ACGGCCTGGA TCAATGACCA CAAAAACGTG GCCATCGATT TTCCGCAGTT CAATGCCCGC 
GAGGAAGTGC GTCAGATCAA GCACGGGGAG CTGCGAACGG CAGTGATCTC TGGCGATGAG 
CGGACTGTGC GGGTGCTGCT GGCAGCTCTT GGAACTGAGC GTCAGATTAT AGTCAATATG 
GCTCCTTCAG GAGCAAACAC CCTTCTATTC CTAGCCTGCC AGTCTGGTTA CGAGAGCATT 
ACACAGCGAT TGCTGGATGC TGGAGCGGAT GGTCGCTCCC ATGCCGTGAC CAAGTACTCA 
CCCTTGTACG CCGCCGTCCA TAGCGGTCAC TTGGGCATTG CCCGACTGAT GCTAGACCAT 
TTTCCAGAAC TGATCCAGCA GCCGACTGTA GAGCGCTGGC TGCCGCTGCA CGCCGCCTGC 
ATCAATGGAC ACATCAAGCT GCTGGAACTC CTTATCAGCT ACAGTTATCC CGACTACCTC 
TACCAGACAT ATCGCGACGA GGAGGGCCAG TGGGAATGGC GGCTCCCCTT CGACGCTAAC 
GCTCATGATG TGACGGGTCA GACGAGTCTG TATATTGCCA GCATCCTAGG AAACAAGCAG 
CTGGTTGGTG TGCTTCTAAA GTGGCAGCTC CATTGCCGGC GTACGTTGGG CGATTCCGCC 
AGCTCGGTGA GCACTCCTAT TACGCCCACC AGGAAACGTA TTTCATTTGG CATTCAGGCT 
ATCATGTCCA AACTGCACAT ATCTGGAGAG TCAGAAGGAC CCGACGACCT AGCTTCACAA 
GAGTCAACCG AGTGCCAACG GTGTCCCATT AACGTGAATC TGCTATGTGG AGCGGCGAGA 
GAAACAGCTC TGCTTGCGGC CGTTCGAGGC GGCCACCTAG ACGTGGTGCA GTCTCTGCTA 
CAGCACGGCG CAAATCCGAA TATTGTAGCC AAGCCAGTTG AGGATCACAA CGACCCGAAA 
TGTTGCGAGG AAATATATGG GCTCAGTAAT GTCCCCATTG CAGAGGCCTG CAAGCAAAGG 
TCGCTGGCTA TGTTGGATCT CTTGCTTAAG CATGGAGCCC GCGACGATAA TGGCACGGCC 
ATTGGAATGG CTATTACATG CGGCGACGAG GCCATCCTGA GTCGTCTTTT GGCCCGACGA 
GTCCATCCGG ACTCAGACTA CAAGATCAAC AAGAAGGGTC TTCCCACACC AGTGGAGGTG 
AACGTGTTTT TGCCGTCCAC CAGCAACATA TCTTACAGTG CTATGTTCCC CAACAATCCA 
ACCATTATTG ACTGGCACAG CATGGGCTCC AGTGTCCAGT TGTCTGTTGT AAGGGTTCCC 
TGGATGGTCA GCGGTGTGTT GCTGCTGAAT CCTAAGCTAC AGTCGCATCC GCGACTTAAT 
GAGGTTGCTC TGACAGCTAT CACGCGAATA GATTTCTCGC ACAACGTTCT CACGTCAATT 
CCACAGGAGC TTTTCCATTT GGTTAGCCTA AGGTATTTAA ATGTGGCGCA AAACAAGATA 
ACCGATCTAC CTGCACCAAT AGGCCAGACA TATGGGTGTC CGGTACTGGA TGAACTCTTT 
CTACAAGACA ACCAGTTAAC TACGCTGCCA GCCGCCATCT TTCACCTGCC CGCCCTATCT 
ATATTGGATG TTTCAAATAA CAAACTGCAA CAGCTTCCCT TCGACCTGTG GCGTGCACCC 
AAGTTGCGCG AACTTAATGT GGCCTTCAAC CTATTGCGGG ATCTTCCTGT GCCGCCAATG 
CAAACTAGCA GTTCGCTGCT GAGCCTAGAC AAATTGAATC TGCAATCTTT TGAGGAGCCG 
CCATCAAACA AACCGCGAAA TGTGACTCAG CAACGTCTCA CCCATCGCAA TCTATGGTCG 
GCTACTTTGG ACATAACCGA TAATGACATG AAGTGGCAGC ATGAACAAGA TTTAGGCGAT 
GGAAAGACAG CTGGTGTGGG CTCCTCCCAG CTAAGTAGCT TAAATATAGC AAATAACCTC 
TTCACCAGTA TCCCCGCTGC TTTGCCCTGT CTGGCTGTAA ACCTAACACG GCTGAATATG 
TCATACAACA GTCTGCGTTC TATGGGACAT GTAACCAGTT ACCCGGCAAC ACTTAAGCAG 
TTGGATCTAA GTCACAACGA GATATCCTGC TGGCCAAGTT TACCGCGAAT TACTGAATCG 
GATCCGCATC TACTGTGCTA CAGTTGTGTC CAGCTACCAG AGGGTCGAGA TGACGACTAC 
AAGACGGCCT CCTCAAAGGG CAGTAGCTCG TCTGCCACAT CGTTTCGCGC TTCGGTGTTA 
AAAAGTGTTT GTCGGCACAG GCGTCATCTG CGCCTGGAGG CTCTACGCAC ATTGATCTTG 
GCTGACAATC TGCTTACGCG CATCCAACTA TCAACTGATG ACGCCACAAC CCTTTTCAAT 
GAAAGTGAGG ACGCCGATTG GAGTGTGGTA GGCGTGAACC GGTCTAAGGT GATATTTCCC 
AACCTGTCTA TGCTGGATAT GACCAACAAT TGCCTGAAGG AGATTCCCGC CTCGTTGCAC 
GAACTGAGCA GTCTGTCAGT GCTGAATATT AGTGGCAACG TCAACATCAC GGAACTGCCG 
CCGCACTTGG GACTGCTGTC GAGACTTTGG AATCTTAATA CGCGCGGTTG CCTACTGCAG 
GAGCCACTGC GCTCTATGAT CGAGAGCAAG AAGCACAAGA CGATGGACAT TGTTGGATAT 
CTTAAATCTA TTTACGAAGA TGCTCAGCCC TACGCACGGA TGAAACTGAT GGTGGTTGGT 
GTGGCTGGAA TTGGCAAGAG CACTCTGCTG GACTTACTGC GCCAGGGGGC GGGCTCAGGG 
TCCAGCTCCA GCTCTCATCG ATCGCGCGCC AGCGAAAATC ATTGGGCCAA GCGAATGGGA 
CACGCACGCA GCACATCTCG ATCTCATCGC CATTCGTCGG CTTCTAGCGC AAACATTTCT 
ACAGTAGGAG TGGACATTGG AACATGGATA TGTGAGAAAC GAAAGCGAGC ACCCGGCTCC 
CACGGCCCAG TGGTGTTTCG TACTTGGGAT TTTGGTGGTC AGAAGGAGTA CTACGCCACA 
CATCAGTACT TTTTGTCCAA ACGGAGTCTG TATTTGGTGC TCTGGCGCAT TAGCGACGGT 
CACAAGGGTC TGGCGGAGTT GCTGCAGTGG CTTGGCAACA TTCAAGCGAG GGCTCCCAAC 
TCCCCCGTAA TCATTGTGGG CACACACTTT GATGCCGTTG GTGAATCCAT CTCGCCCCAA 
AAGGCAGAGC AACTGCAGCA ATTGATTCGG GAGAAGTTTA TCGCCATTCC CGATGCGGAA 
AAAATTGGGC TGCCACGGGT GATTGATTCC ATCGAAATAA GTTGCCGGAC CCTACACAAT 
ATCCACTTAT TGGCCAACAT TATTTACGAC ACCGCCATGC AACTGCGATC CCCTGGTTCC 
AAGGAGCCCA TGCTACTGCA AAAGATCCCC GCCAGCTACA TTGCCTTGGA GGATATTGTG 
AATGTGATTG CATGCAATCT GCGCGCTGCT GGGCGAGATC CCGTCCTGGA TGGTGAACAA 
TATAAGCGCT TGGTCACCGA ACAGATGAGA TTGCACAACT ACAAGAGCTT CCGGGATGCC 
GCTGAGCTGC AGCAGGCCAC TACATGGTGC CACGAAAATG GAGTTTTATT GCACTATGAT 
GATGCTACGC TAAGGGACTA CTACTTTCTC GATCCACAGT GGCTTTGCGA CATGCTGGCC 
CATGTGGTCA CCGTGCGGGA GATCAACCCC TTTGCTCCTA CGGGCGTGAT GAAGCTGGAT 
GATCTCCAGA TGTTGTTCCG AAGTGTGCAG GTTCAAGGAA ATGGAAACAG AAGCTACATT 
GTCAGTCTAC TTAACAAGTT TGAGGTTGCT TTAACGTGGG ATTCGAGAAC TCTACTCATA 
CCCTCCTTAC TGCCTTCGCA GGAGGCAGCT ACACCCAATT CAGGAAGCAC GGTTAAGCTA 
TCGCAACGAT CTCGCGGCCG TAGTTTGGGT TGCTCTGTAT CGCAAGAAGT AAATCTAAAT 
AATTTGATAT ACGAACAGAG GTCAGCTCCT TCTTCATCAT CTTCCTCTGC ATCGGTTAGC 
CAAGGCTTGC GGCGCATCCT ATTGATGACT TATTTCCCGT CCGGGTTTTG GTCAAGATTA 
ATCACCCGGA TTTTAGCTGA TGAACAAATA ATCGAAGCAA TTCGTGGCGT TTATATGGCT 
TCACAAGATT ACGCGGACTT TGATCTACGC ACATCGTTGG AGCAGGACAC CCAATGGAAT 
CTTTGGCAGA CAGGCTTAGC ACTGTACTAT GGACCTATCT TAATATTCAA GATCTGGGAA 
GTACCCTTTC AGAAGACAGA GCGAACGCAA CCTTTCCGCA CTGATGGCAA CCGATTTAAG 
CTGAAACAAG ATGGTATCTG GAGCGATGTA AATCTCAGCT CCTCTAGCAT TTTGGAAGTT 
TACTTTCCAC TCTATGAAGT GAACATATCG CAAGAAGTGG ATGACAATGA ACGTCAATTG 
CTGGCCGAGA TTCGTCCGCA CATGTCTCAG GTGGCCAAGC TTTTGGCCCT AACAGTGGAT 
CATATTGACT TGCTTTTGGA GGATTGGTAT CCTTCATTGG GAACGCGGTT TGTGCACACT 
TCCGAGGGTC GATTCCTAAT CACTCGATTG GTGTTGTGTC CGCGATGTCT TTGGAAGTTG 
CAGCTGCAGC AGAACAACGA ACCGTCTGAT CGGGAAGTGC CTCCAGTGGG TTGCAATCGA 
CCAAGTCGAA GTAGTAGGCG TGGAGCGGGA GCATACTTTC TCCACGGTGT AGGTGATCCC 
GGAGAGGATG GTGCTTTAAA CGTGTTCTCG GCCTATTTAA ACGCCACAGC AAGGAGAGAA 
CGCCGATCGG AGGATTCTTT GGGTGCTGGA TCCGACGCGG ACTCGGGCGT GGGTCCCGAC 
TCAGCCGGTT CTTCGCGCAA CACTTCAGTT GATGGTCATC CCGGATATCA CTTGCCTGAT 
AACTCAAATG TGTGTTATGC TTGGATGATT GAGGAGTGCA TTTTGTCCGT TTATAATCAA 
AGCAAGATTA GTTGCCCTGT TCATCTTGAA CAGTCTATGG CTCAGCTTGC GCCAGACGTA 
ATCTTTGCCG ATATTCCTGA TAAGCACACT ATTCCAAGCG AGTGCATCAT CAAGGGATCG 
TTGCTTGGTC GAGGTGCTTT TGGGTTCGTT TTTAAAGCAA ATTGCAAGGT AAGGGGCGCC 
AGATCCTTTA AGCCGGTGGC AATGAAAATG CTTCAGCCAG TGCCTCCCGG AGCTCGTGCA 
AAAGAGAGCG CTTTGATGGC TTTCAAGGTA GCTGTAGGCA AATGGGACCG GGATCCACTG 
CAGCACTCTT GCAAGGCCTA CTGCACTGCG CGTCAGGAAT TGGCAGTACT TCTAACTCTC 
AAGCATCCAA ACATTGTACC CTTGGTCGGG ATCTGCATTA AACCATTAGC TCTGGTTCTG 
GAACTAGCTC CTCTAGGCGG TTTGGACGCT TTGCTCCGGC ATTACCGACG CAGCGGAGCC 
CACATGGGAC CGCATACTTT CCAGACCCTT GTCCTGCAGG CGGCTCGAGC AATCGAGTAT 
TTGCATCGCA GAAGAATCAT CTACCGCGAT CTAAAGTCTG AAAATGTCTT GGTTTGGGAG 
CTTCCACAAC CACACACGGA AGACAGTCCC CGCAACCTTG TGCACATAAA GATTGCCGAC 
TACGGAATTA GCCGGCAAAC TGCTCCAAGT GGAGCTAAAG GATTTGGTGG CACCGAGGGT 
TTTATGGCTC CGGAGATCAT ACGCTACAAT GGTGAGGAGG AATATACTGA AAAGGTGGAC 
TGCTTCTCAT TTGGAATGTT CATATATGAG AATATCAGTT TACGACAACC TTTCGAAGGC 
CACGAGTCTA TTAAAGAGTG CATCTTGGAG GGTAGTCGCC CGGCTTTGAC TCAAAGGGAA 
ACCCAGTTTC CCACCTGCTG TTTGGATCTT ATGGTCTTGT GTTGGCACGA ACAGCCTCGT 
CGCAGACCGA CGGCAAGTCA GATTGTTTCC ATACTAAGTG CACCGGAGTG CATCCACCTC 
CTGGATGTCG TTGCCATGCC GCATAGCGAG AAGATTGTCT GTGGGGTTTT TCAGTCGCTT 
GTTGGTATGG GCGATGATGA ACGATGTGGT CTTGAACTGT GGCTACCCTC CTTCGGCTCT 
CGCATTGACA TTCTAGACTG CTCACCCTCG GGCAGCCTAC TGCAGTGCAA CAGCATCAGT 
TGTTCTCCGC AGCCACAGGT TGCTCCGCCC AAGACACCCG AAAACGGTGC AAATTCACGT 
GCCCGCTCTG CCCAACGTTT GCCCAAGATG AACATGTTGT GCTGCTGCCT GGTGGGTGAA 
GCCATCTGGA TGGGCGACGT TTCCGGCAAT CTGCACGCCT ATAGCACATC CACCTATGCC 
CACCTGTTTT CCTACATGCT CGATCCCAAC ATTAAGTCAG CTGTGATCAG TCTAGTCTAC 
ATGGAGAAAA TAGCTCGCGT GGCTGTCGGA ACGCATAACG GTCGGGTGTT CCTTGTTGAC 
GCCACGCAGA TGCCCAGCAA TTGCGCATTT GCCGAGGGCT CTTTTGTGCT TACAGAGATC 
TGTTCTGGTT TTGTGTTGCA CGCCGCTTGT TCCGTTGTTG TGGATGGAAT CTATGAATTG 
TGGTGCGGCG AAATTGCAGG AAAGATAAAT GTTTTTCCGT TGAACGAGAA TGGAGTTTCC 
GGACATCAGG CTTTATGCCA CAGCGAGGAA CCCAATCTCA TCGAGGACGT CAAGGTGGCT 
CGAATGTGCA GTAATGAAAG CCATGTGTTC AGTTGTCTAT ATCCCGGTTG TATGGTGTAC 
CAGTGGGATG TTATCTCCAA GCGGATAGAA AATAAGCTGG ACTGCTCAAA GCTGCTGCCC 
TGCTCCGAGT CTCTGCAAAG TATTGCGATT GATGAGCATG TGAACCTTAT AAAATGCCAG 
ATTTCGGCAC TGGCGGCTCA CAATTCAGAA CTGTATATTG GGACGACCTG GGGCTGCTTA 
ATTGTGGCCG AACTGCACAC CCTGCGCCCC ATTAGTGTTT TCCGACCCTA TGAAAATGAG 
ATCAAATCTA TAATAACCCT TTCTAAAGAC AATGTTCCGC TGATCGCCAC GATCGGAAGA 
CGATATCGCT CTCTGATCTC TCGATATGTG GACTCCGCAG AGTCATCCAC CAAGAGCTCT 
GCCGTCAGCA CACCCACCCA TGGTGCAGCC AAATCCGTGC CACCGGCGGA TGTGGATAAT 
CACATTCATT GCCTGCTGTG GCGCGCCAAG CACTGGACCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262772.1
CDS	159..7700
Translation	MSDSDEARAQGDIGAMEVGAQKLFSNSHPPQKQLVQPLDHPTPTKRQIKTNATHSPNATFLNKCNSCRASWAPTCAVRHKTAWINDHKNVAIDFPQFNAREEVRQIKHGELRTAVISGDERTVRVLLAALGTERQIIVNMAPSGANTLLFLACQSGYESITQRLLDAGADGRSHAVTKYSPLYAAVHSGHLGIARLMLDHFPELIQQPTVERWLPLHAACINGHIKLLELLISYSYPDYLYQTYRDEEGQWEWRLPFDANAHDVTGQTSLYIASILGNKQLVGVLLKWQLHCRRTLGDSASSVSTPITPTRKRISFGIQAIMSKLHISGESEGPDDLASQESTECQRCPINVNLLCGAARETALLAAVRGGHLDVVQSLLQHGANPNIVAKPVEDHNDPKCCEEIYGLSNVPIAEACKQRSLAMLDLLLKHGARDDNGTAIGMAITCGDEAILSRLLARRVHPDSDYKINKKGLPTPVEVNVFLPSTSNISYSAMFPNNPTIIDWHSMGSSVQLSVVRVPWMVSGVLLLNPKLQSHPRLNEVALTAITRIDFSHNVLTSIPQELFHLVSLRYLNVAQNKITDLPAPIGQTYGCPVLDELFLQDNQLTTLPAAIFHLPALSILDVSNNKLQQLPFDLWRAPKLRELNVAFNLLRDLPVPPMQTSSSLLSLDKLNLQSFEEPPSNKPRNVTQQRLTHRNLWSATLDITDNDMKWQHEQDLGDGKTAGVGSSQLSSLNIANNLFTSIPAALPCLAVNLTRLNMSYNSLRSMGHVTSYPATLKQLDLSHNEISCWPSLPRITESDPHLLCYSCVQLPEGRDDDYKTASSKGSSSSATSFRASVLKSVCRHRRHLRLEALRTLILADNLLTRIQLSTDDATTLFNESEDADWSVVGVNRSKVIFPNLSMLDMTNNCLKEIPASLHELSSLSVLNISGNVNITELPPHLGLLSRLWNLNTRGCLLQEPLRSMIESKKHKTMDIVGYLKSIYEDAQPYARMKLMVVGVAGIGKSTLLDLLRQGAGSGSSSSSHRSRASENHWAKRMGHARSTSRSHRHSSASSANISTVGVDIGTWICEKRKRAPGSHGPVVFRTWDFGGQKEYYATHQYFLSKRSLYLVLWRISDGHKGLAELLQWLGNIQARAPNSPVIIVGTHFDAVGESISPQKAEQLQQLIREKFIAIPDAEKIGLPRVIDSIEISCRTLHNIHLLANIIYDTAMQLRSPGSKEPMLLQKIPASYIALEDIVNVIACNLRAAGRDPVLDGEQYKRLVTEQMRLHNYKSFRDAAELQQATTWCHENGVLLHYDDATLRDYYFLDPQWLCDMLAHVVTVREINPFAPTGVMKLDDLQMLFRSVQVQGNGNRSYIVSLLNKFEVALTWDSRTLLIPSLLPSQEAATPNSGSTVKLSQRSRGRSLGCSVSQEVNLNNLIYEQRSAPSSSSSSASVSQGLRRILLMTYFPSGFWSRLITRILADEQIIEAIRGVYMASQDYADFDLRTSLEQDTQWNLWQTGLALYYGPILIFKIWEVPFQKTERTQPFRTDGNRFKLKQDGIWSDVNLSSSSILEVYFPLYEVNISQEVDDNERQLLAEIRPHMSQVAKLLALTVDHIDLLLEDWYPSLGTRFVHTSEGRFLITRLVLCPRCLWKLQLQQNNEPSDREVPPVGCNRPSRSSRRGAGAYFLHGVGDPGEDGALNVFSAYLNATARRERRSEDSLGAGSDADSGVGPDSAGSSRNTSVDGHPGYHLPDNSNVCYAWMIEECILSVYNQSKISCPVHLEQSMAQLAPDVIFADIPDKHTIPSECIIKGSLLGRGAFGFVFKANCKVRGARSFKPVAMKMLQPVPPGARAKESALMAFKVAVGKWDRDPLQHSCKAYCTARQELAVLLTLKHPNIVPLVGICIKPLALVLELAPLGGLDALLRHYRRSGAHMGPHTFQTLVLQAARAIEYLHRRRIIYRDLKSENVLVWELPQPHTEDSPRNLVHIKIADYGISRQTAPSGAKGFGGTEGFMAPEIIRYNGEEEYTEKVDCFSFGMFIYENISLRQPFEGHESIKECILEGSRPALTQRETQFPTCCLDLMVLCWHEQPRRRPTASQIVSILSAPECIHLLDVVAMPHSEKIVCGVFQSLVGMGDDERCGLELWLPSFGSRIDILDCSPSGSLLQCNSISCSPQPQVAPPKTPENGANSRARSAQRLPKMNMLCCCLVGEAIWMGDVSGNLHAYSTSTYAHLFSYMLDPNIKSAVISLVYMEKIARVAVGTHNGRVFLVDATQMPSNCAFAEGSFVLTEICSGFVLHAACSVVVDGIYELWCGEIAGKINVFPLNENGVSGHQALCHSEEPNLIEDVKVARMCSNESHVFSCLYPGCMVYQWDVISKRIENKLDCSKLLPCSESLQSIAIDEHVNLIKCQISALAAHNSELYIGTTWGCLIVAELHTLRPISVFRPYENEIKSIITLSKDNVPLIATIGRRYRSLISRYVDSAESSTKSSAVSTPTHGAAKSVPPADVDNHIHCLLWRAKHWT

Target ORF information:

RefSeq Version	NM_001275843.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Leucine-rich repeat kinase (Lrrk), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275843.1

ORF Insert Sequence:

ATGAGCGACT CCGACGAAGC AAGGGCACAG GGTGACATTG GTGCAATGGA AGTGGGCGCG 
CAAAAACTTT TTTCAAACAG CCACCCCCCA CAGAAGCAAC TGGTGCAACC ACTGGACCAT 
CCGACACCCA CGAAGAGACA AATCAAAACA AATGCCACGC ACTCACCCAA TGCCACATTC 
TTGAACAAGT GCAACAGTTG CCGCGCTTCT TGGGCTCCGA CTTGCGCAGT GCGACATAAA 
ACGGCCTGGA TCAATGACCA CAAAAACGTG GCCATCGATT TTCCGCAGTT CAATGCCCGC 
GAGGAAGTGC GTCAGATCAA GCACGGGGAG CTGCGAACGG CAGTGATCTC TGGCGATGAG 
CGGACTGTGC GGGTGCTGCT GGCAGCTCTT GGAACTGAGC GTCAGATTAT AGTCAATATG 
GCTCCTTCAG GAGCAAACAC CCTTCTATTC CTAGCCTGCC AGTCTGGTTA CGAGAGCATT 
ACACAGCGAT TGCTGGATGC TGGAGCGGAT GGTCGCTCCC ATGCCGTGAC CAAGTACTCA 
CCCTTGTACG CCGCCGTCCA TAGCGGTCAC TTGGGCATTG CCCGACTGAT GCTAGACCAT 
TTTCCAGAAC TGATCCAGCA GCCGACTGTA GAGCGCTGGC TGCCGCTGCA CGCCGCCTGC 
ATCAATGGAC ACATCAAGCT GCTGGAACTC CTTATCAGCT ACAGTTATCC CGACTACCTC 
TACCAGACAT ATCGCGACGA GGAGGGCCAG TGGGAATGGC GGCTCCCCTT CGACGCTAAC 
GCTCATGATG TGACGGGTCA GACGAGTCTG TATATTGCCA GCATCCTAGG AAACAAGCAG 
CTGGTTGGTG TGCTTCTAAA GTGGCAGCTC CATTGCCGGC GTACGTTGGG CGATTCCGCC 
AGCTCGGTGA GCACTCCTAT TACGCCCACC AGGAAACGTA TTTCATTTGG CATTCAGGCT 
ATCATGTCCA AACTGCACAT ATCTGGAGAG TCAGAAGGAC CCGACGACCT AGCTTCACAA 
GAGTCAACCG AGTGCCAACG GTGTCCCATT AACGTGAATC TGCTATGTGG AGCGGCGAGA 
GAAACAGCTC TGCTTGCGGC CGTTCGAGGC GGCCACCTAG ACGTGGTGCA GTCTCTGCTA 
CAGCACGGCG CAAATCCGAA TATTGTAGCC AAGCCAGTTG AGGATCACAA CGACCCGAAA 
TGTTGCGAGG AAATATATGG GCTCAGTAAT GTCCCCATTG CAGAGGCCTG CAAGCAAAGG 
TCGCTGGCTA TGTTGGATCT CTTGCTTAAG CATGGAGCCC GCGACGATAA TGGCACGGCC 
ATTGGAATGG CTATTACATG CGGCGACGAG GCCATCCTGA GTCGTCTTTT GGCCCGACGA 
GTCCATCCGG ACTCAGACTA CAAGATCAAC AAGAAGGGTC TTCCCACACC AGTGGAGGTG 
AACGTGTTTT TGCCGTCCAC CAGCAACATA TCTTACAGTG CTATGTTCCC CAACAATCCA 
ACCATTATTG ACTGGCACAG CATGGGCTCC AGTGTCCAGT TGTCTGTTGT AAGGGTTCCC 
TGGATGGTCA GCGGTGTGTT GCTGCTGAAT CCTAAGCTAC AGTCGCATCC GCGACTTAAT 
GAGGTTGCTC TGACAGCTAT CACGCGAATA GATTTCTCGC ACAACGTTCT CACGTCAATT 
CCACAGGAGC TTTTCCATTT GGTTAGCCTA AGGTATTTAA ATGTGGCGCA AAACAAGATA 
ACCGATCTAC CTGCACCAAT AGGCCAGACA TATGGGTGTC CGGTACTGGA TGAACTCTTT 
CTACAAGACA ACCAGTTAAC TACGCTGCCA GCCGCCATCT TTCACCTGCC CGCCCTATCT 
ATATTGGATG TTTCAAATAA CAAACTGCAA CAGCTTCCCT TCGACCTGTG GCGTGCACCC 
AAGTTGCGCG AACTTAATGT GGCCTTCAAC CTATTGCGGG ATCTTCCTGT GCCGCCAATG 
CAAACTAGCA GTTCGCTGCT GAGCCTAGAC AAATTGAATC TGCAATCTTT TGAGGAGCCG 
CCATCAAACA AACCGCGAAA TGTGACTCAG CAACGTCTCA CCCATCGCAA TCTATGGTCG 
GCTACTTTGG ACATAACCGA TAATGACATG AAGTGGCAGC ATGAACAAGA TTTAGGCGAT 
GGAAAGACAG CTGGTGTGGG CTCCTCCCAG CTAAGTAGCT TAAATATAGC AAATAACCTC 
TTCACCAGTA TCCCCGCTGC TTTGCCCTGT CTGGCTGTAA ACCTAACACG GCTGAATATG 
TCATACAACA GTCTGCGTTC TATGGGACAT GTAACCAGTT ACCCGGCAAC ACTTAAGCAG 
TTGGATCTAA GTCACAACGA GATATCCTGC TGGCCAAGTT TACCGCGAAT TACTGAATCG 
GATCCGCATC TACTGTGCTA CAGTTGTGTC CAGCTACCAG AGGGTCGAGA TGACGACTAC 
AAGACGGCCT CCTCAAAGGG CAGTAGCTCG TCTGCCACAT CGTTTCGCGC TTCGGTGTTA 
AAAAGTGTTT GTCGGCACAG GCGTCATCTG CGCCTGGAGG CTCTACGCAC ATTGATCTTG 
GCTGACAATC TGCTTACGCG CATCCAACTA TCAACTGATG ACGCCACAAC CCTTTTCAAT 
GAAAGTGAGG ACGCCGATTG GAGTGTGGTA GGCGTGAACC GGTCTAAGGT GATATTTCCC 
AACCTGTCTA TGCTGGATAT GACCAACAAT TGCCTGAAGG AGATTCCCGC CTCGTTGCAC 
GAACTGAGCA GTCTGTCAGT GCTGAATATT AGTGGCAACG TCAACATCAC GGAACTGCCG 
CCGCACTTGG GACTGCTGTC GAGACTTTGG AATCTTAATA CGCGCGGTTG CCTACTGCAG 
GAGCCACTGC GCTCTATGAT CGAGAGCAAG AAGCACAAGA CGATGGACAT TGTTGGATAT 
CTTAAATCTA TTTACGAAGA TGCTCAGCCC TACGCACGGA TGAAACTGAT GGTGGTTGGT 
GTGGCTGGAA TTGGCAAGAG CACTCTGCTG GACTTACTGC GCCAGGGGGC GGGCTCAGGG 
TCCAGCTCCA GCTCTCATCG ATCGCGCGCC AGCGAAAATC ATTGGGCCAA GCGAATGGGA 
CACGCACGCA GCACATCTCG ATCTCATCGC CATTCGTCGG CTTCTAGCGC AAACATTTCT 
ACAGTAGGAG TGGACATTGG AACATGGATA TGTGAGAAAC GAAAGCGAGC ACCCGGCTCC 
CACGGCCCAG TGGTGTTTCG TACTTGGGAT TTTGGTGGTC AGAAGGAGTA CTACGCCACA 
CATCAGTACT TTTTGTCCAA ACGGAGTCTG TATTTGGTGC TCTGGCGCAT TAGCGACGGT 
CACAAGGGTC TGGCGGAGTT GCTGCAGTGG CTTGGCAACA TTCAAGCGAG GGCTCCCAAC 
TCCCCCGTAA TCATTGTGGG CACACACTTT GATGCCGTTG GTGAATCCAT CTCGCCCCAA 
AAGGCAGAGC AACTGCAGCA ATTGATTCGG GAGAAGTTTA TCGCCATTCC CGATGCGGAA 
AAAATTGGGC TGCCACGGGT GATTGATTCC ATCGAAATAA GTTGCCGGAC CCTACACAAT 
ATCCACTTAT TGGCCAACAT TATTTACGAC ACCGCCATGC AACTGCGATC CCCTGGTTCC 
AAGGAGCCCA TGCTACTGCA AAAGATCCCC GCCAGCTACA TTGCCTTGGA GGATATTGTG 
AATGTGATTG CATGCAATCT GCGCGCTGCT GGGCGAGATC CCGTCCTGGA TGGTGAACAA 
TATAAGCGCT TGGTCACCGA ACAGATGAGA TTGCACAACT ACAAGAGCTT CCGGGATGCC 
GCTGAGCTGC AGCAGGCCAC TACATGGTGC CACGAAAATG GAGTTTTATT GCACTATGAT 
GATGCTACGC TAAGGGACTA CTACTTTCTC GATCCACAGT GGCTTTGCGA CATGCTGGCC 
CATGTGGTCA CCGTGCGGGA GATCAACCCC TTTGCTCCTA CGGGCGTGAT GAAGCTGGAT 
GATCTCCAGA TGTTGTTCCG AAGTGTGCAG GTTCAAGGAA ATGGAAACAG AAGCTACATT 
GTCAGTCTAC TTAACAAGTT TGAGGTTGCT TTAACGTGGG ATTCGAGAAC TCTACTCATA 
CCCTCCTTAC TGCCTTCGCA GGAGGCAGCT ACACCCAATT CAGGAAGCAC GGTTAAGCTA 
TCGCAACGAT CTCGCGGCCG TAGTTTGGGT TGCTCTGTAT CGCAAGAAGT AAATCTAAAT 
AATTTGATAT ACGAACAGAG GTCAGCTCCT TCTTCATCAT CTTCCTCTGC ATCGGTTAGC 
CAAGGCTTGC GGCGCATCCT ATTGATGACT TATTTCCCGT CCGGGTTTTG GTCAAGATTA 
ATCACCCGGA TTTTAGCTGA TGAACAAATA ATCGAAGCAA TTCGTGGCGT TTATATGGCT 
TCACAAGATT ACGCGGACTT TGATCTACGC ACATCGTTGG AGCAGGACAC CCAATGGAAT 
CTTTGGCAGA CAGGCTTAGC ACTGTACTAT GGACCTATCT TAATATTCAA GATCTGGGAA 
GTACCCTTTC AGAAGACAGA GCGAACGCAA CCTTTCCGCA CTGATGGCAA CCGATTTAAG 
CTGAAACAAG ATGGTATCTG GAGCGATGTA AATCTCAGCT CCTCTAGCAT TTTGGAAGTT 
TACTTTCCAC TCTATGAAGT GAACATATCG CAAGAAGTGG ATGACAATGA ACGTCAATTG 
CTGGCCGAGA TTCGTCCGCA CATGTCTCAG GTGGCCAAGC TTTTGGCCCT AACAGTGGAT 
CATATTGACT TGCTTTTGGA GGATTGGTAT CCTTCATTGG GAACGCGGTT TGTGCACACT 
TCCGAGGGTC GATTCCTAAT CACTCGATTG GTGTTGTGTC CGCGATGTCT TTGGAAGTTG 
CAGCTGCAGC AGAACAACGA ACCGTCTGAT CGGGAAGTGC CTCCAGTGGG TTGCAATCGA 
CCAAGTCGAA GTAGTAGGCG TGGAGCGGGA GCATACTTTC TCCACGGTGT AGGTGATCCC 
GGAGAGGATG GTGCTTTAAA CGTGTTCTCG GCCTATTTAA ACGCCACAGC AAGGAGAGAA 
CGCCGATCGG AGGATTCTTT GGGTGCTGGA TCCGACGCGG ACTCGGGCGT GGGTCCCGAC 
TCAGCCGGTT CTTCGCGCAA CACTTCAGTT GATGGTCATC CCGGATATCA CTTGCCTGAT 
AACTCAAATG TGTGTTATGC TTGGATGATT GAGGAGTGCA TTTTGTCCGT TTATAATCAA 
AGCAAGATTA GTTGCCCTGT TCATCTTGAA CAGTCTATGG CTCAGCTTGC GCCAGACGTA 
ATCTTTGCCG ATATTCCTGA TAAGCACACT ATTCCAAGCG AGTGCATCAT CAAGGGATCG 
TTGCTTGGTC GAGGTGCTTT TGGGTTCGTT TTTAAAGCAA ATTGCAAGGT AAGGGGCGCC 
AGATCCTTTA AGCCGGTGGC AATGAAAATG CTTCAGCCAG TGCCTCCCGG AGCTCGTGCA 
AAAGAGAGCG CTTTGATGGC TTTCAAGGTA GCTGTAGGCA AATGGGACCG GGATCCACTG 
CAGCACTCTT GCAAGGCCTA CTGCACTGCG CGTCAGGAAT TGGCAGTACT TCTAACTCTC 
AAGCATCCAA ACATTGTACC CTTGGTCGGG ATCTGCATTA AACCATTAGC TCTGGTTCTG 
GAACTAGCTC CTCTAGGCGG TTTGGACGCT TTGCTCCGGC ATTACCGACG CAGCGGAGCC 
CACATGGGAC CGCATACTTT CCAGACCCTT GTCCTGCAGG CGGCTCGAGC AATCGAGTAT 
TTGCATCGCA GAAGAATCAT CTACCGCGAT CTAAAGTCTG AAAATGTCTT GGTTTGGGAG 
CTTCCACAAC CACACACGGA AGACAGTCCC CGCAACCTTG TGCACATAAA GATTGCCGAC 
TACGGAATTA GCCGGCAAAC TGCTCCAAGT GGAGCTAAAG GATTTGGTGG CACCGAGGGT 
TTTATGGCTC CGGAGATCAT ACGCTACAAT GGTGAGGAGG AATATACTGA AAAGGTGGAC 
TGCTTCTCAT TTGGAATGTT CATATATGAG AATATCAGTT TACGACAACC TTTCGAAGGC 
CACGAGTCTA TTAAAGAGTG CATCTTGGAG GGTAGTCGCC CGGCTTTGAC TCAAAGGGAA 
ACCCAGTTTC CCACCTGCTG TTTGGATCTT ATGGTCTTGT GTTGGCACGA ACAGCCTCGT 
CGCAGACCGA CGGCAAGTCA GATTGTTTCC ATACTAAGTG CACCGGAGTG CATCCACCTC 
CTGGATGTCG TTGCCATGCC GCATAGCGAG AAGATTGTCT GTGGGGTTTT TCAGTCGCTT 
GTTGGTATGG GCGATGATGA ACGATGTGGT CTTGAACTGT GGCTACCCTC CTTCGGCTCT 
CGCATTGACA TTCTAGACTG CTCACCCTCG GGCAGCCTAC TGCAGTGCAA CAGCATCAGT 
TGTTCTCCGC AGCCACAGGT TGCTCCGCCC AAGACACCCG AAAACGGTGC AAATTCACGT 
GCCCGCTCTG CCCAACGTTT GCCCAAGATG AACATGTTGT GCTGCTGCCT GGTGGGTGAA 
GCCATCTGGA TGGGCGACGT TTCCGGCAAT CTGCACGCCT ATAGCACATC CACCTATGCC 
CACCTGTTTT CCTACATGCT CGATCCCAAC ATTAAGTCAG CTGTGATCAG TCTAGTCTAC 
ATGGAGAAAA TAGCTCGCGT GGCTGTCGGA ACGCATAACG GTCGGGTGTT CCTTGTTGAC 
GCCACGCAGA TGCCCAGCAA TTGCGCATTT GCCGAGGGCT CTTTTGTGCT TACAGAGATC 
TGTTCTGGTT TTGTGTTGCA CGCCGCTTGT TCCGTTGTTG TGGATGGAAT CTATGAATTG 
TGGTGCGGCG AAATTGCAGG AAAGATAAAT GTTTTTCCGT TGAACGAGAA TGGAGTTTCC 
GGACATCAGG CTTTATGCCA CAGCGAGGAA CCCAATCTCA TCGAGGACGT CAAGGTGGCT 
CGAATGTGCA GTAATGAAAG CCATGTGTTC AGTTGTCTAT ATCCCGGTTG TATGGTGTAC 
CAGTGGGATG TTATCTCCAA GCGGATAGAA AATAAGCTGG ACTGCTCAAA GCTGCTGCCC 
TGCTCCGAGT CTCTGCAAAG TATTGCGATT GATGAGCATG TGAACCTTAT AAAATGCCAG 
ATTTCGGCAC TGGCGGCTCA CAATTCAGAA CTGTATATTG GGACGACCTG GGGCTGCTTA 
ATTGTGGCCG AACTGCACAC CCTGCGCCCC ATTAGTGTTT TCCGACCCTA TGAAAATGAG 
ATCAAATCTA TAATAACCCT TTCTAAAGAC AATGTTCCGC TGATCGCCAC GATCGGAAGA 
CGATATCGCT CTCTGATCTC TCGATATGTG GACTCCGCAG AGTCATCCAC CAAGAGCTCT 
GCCGTCAGCA CACCCACCCA TGGTGCAGCC AAATCCGTGC CACCGGCGGA TGTGGATAAT 
CACATTCATT GCCTGCTGTG GCGCGCCAAG CACTGGACCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lrrk ( NM_001275842.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262771.1 Drosophila melanogaster Leucine-rich repeat kinase (Lrrk), transcript variant C, mRNA.

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CloneID	OFa07817
Clone ID Related Accession (Same CDS sequence)	NM_001275842.1
Accession Version	NM_001275842.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Leucine-rich repeat kinase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGACT CCGACGAAGA TGCCCGCGAG GAAGTGCGTC AGATCAAGCA CGGGGAGCTG 
CGAACGGCAG TGATCTCTGG CGATGAGCGG ACTGTGCGGG TGCTGCTGGC AGCTCTTGGA 
ACTGAGCGTC AGATTATAGT CAATATGGCT CCTTCAGGAG CAAACACCCT TCTATTCCTA 
GCCTGCCAGT CTGGTTACGA GAGCATTACA CAGCGATTGC TGGATGCTGG AGCGGATGGT 
CGCTCCCATG CCGTGACCAA GTACTCACCC TTGTACGCCG CCGTCCATAG CGGTCACTTG 
GGCATTGCCC GACTGATGCT AGACCATTTT CCAGAACTGA TCCAGCAGCC GACTGTAGAG 
CGCTGGCTGC CGCTGCACGC CGCCTGCATC AATGGACACA TCAAGCTGCT GGAACTCCTT 
ATCAGCTACA GTTATCCCGA CTACCTCTAC CAGACATATC GCGACGAGGA GGGCCAGTGG 
GAATGGCGGC TCCCCTTCGA CGCTAACGCT CATGATGTGA CGGGTCAGAC GAGTCTGTAT 
ATTGCCAGCA TCCTAGGAAA CAAGCAGCTG GTTGGTGTGC TTCTAAAGTG GCAGCTCCAT 
TGCCGGCGTA CGTTGGGCGA TTCCGCCAGC TCGGTGAGCA CTCCTATTAC GCCCACCAGG 
AAACGTATTT CATTTGGCAT TCAGGCTATC ATGTCCAAAC TGCACATATC TGGAGAGTCA 
GAAGGACCCG ACGACCTAGC TTCACAAGAG TCAACCGAGT GCCAACGGTG TCCCATTAAC 
GTGAATCTGC TATGTGGAGC GGCGAGAGAA ACAGCTCTGC TTGCGGCCGT TCGAGGCGGC 
CACCTAGACG TGGTGCAGTC TCTGCTACAG CACGGCGCAA ATCCGAATAT TGTAGCCAAG 
CCAGTTGAGG ATCACAACGA CCCGAAATGT TGCGAGGAAA TATATGGGCT CAGTAATGTC 
CCCATTGCAG AGGCCTGCAA GCAAAGGTCG CTGGCTATGT TGGATCTCTT GCTTAAGCAT 
GGAGCCCGCG ACGATAATGG CACGGCCATT GGAATGGCTA TTACATGCGG CGACGAGGCC 
ATCCTGAGTC GTCTTTTGGC CCGACGAGTC CATCCGGACT CAGACTACAA GATCAACAAG 
AAGGGTCTTC CCACACCAGT GGAGGTGAAC GTGTTTTTGC CGTCCACCAG CAACATATCT 
TACAGTGCTA TGTTCCCCAA CAATCCAACC ATTATTGACT GGCACAGCAT GGGCTCCAGT 
GTCCAGTTGT CTGTTGTAAG GGTTCCCTGG ATGGTCAGCG GTGTGTTGCT GCTGAATCCT 
AAGCTACAGT CGCATCCGCG ACTTAATGAG GTTGCTCTGA CAGCTATCAC GCGAATAGAT 
TTCTCGCACA ACGTTCTCAC GTCAATTCCA CAGGAGCTTT TCCATTTGGT TAGCCTAAGG 
TATTTAAATG TGGCGCAAAA CAAGATAACC GATCTACCTG CACCAATAGG CCAGACATAT 
GGGTGTCCGG TACTGGATGA ACTCTTTCTA CAAGACAACC AGTTAACTAC GCTGCCAGCC 
GCCATCTTTC ACCTGCCCGC CCTATCTATA TTGGATGTTT CAAATAACAA ACTGCAACAG 
CTTCCCTTCG ACCTGTGGCG TGCACCCAAG TTGCGCGAAC TTAATGTGGC CTTCAACCTA 
TTGCGGGATC TTCCTGTGCC GCCAATGCAA ACTAGCAGTT CGCTGCTGAG CCTAGACAAA 
TTGAATCTGC AATCTTTTGA GGAGCCGCCA TCAAACAAAC CGCGAAATGT GACTCAGCAA 
CGTCTCACCC ATCGCAATCT ATGGTCGGCT ACTTTGGACA TAACCGATAA TGACATGAAG 
TGGCAGCATG AACAAGATTT AGGCGATGGA AAGACAGCTG GTGTGGGCTC CTCCCAGCTA 
AGTAGCTTAA ATATAGCAAA TAACCTCTTC ACCAGTATCC CCGCTGCTTT GCCCTGTCTG 
GCTGTAAACC TAACACGGCT GAATATGTCA TACAACAGTC TGCGTTCTAT GGGACATGTA 
ACCAGTTACC CGGCAACACT TAAGCAGTTG GATCTAAGTC ACAACGAGAT ATCCTGCTGG 
CCAAGTTTAC CGCGAATTAC TGAATCGGAT CCGCATCTAC TGTGCTACAG TTGTGTCCAG 
CTACCAGAGG GTCGAGATGA CGACTACAAG ACGGCCTCCT CAAAGGGCAG TAGCTCGTCT 
GCCACATCGT TTCGCGCTTC GGTGTTAAAA AGTGTTTGTC GGCACAGGCG TCATCTGCGC 
CTGGAGGCTC TACGCACATT GATCTTGGCT GACAATCTGC TTACGCGCAT CCAACTATCA 
ACTGATGACG CCACAACCCT TTTCAATGAA AGTGAGGACG CCGATTGGAG TGTGGTAGGC 
GTGAACCGGT CTAAGGTGAT ATTTCCCAAC CTGTCTATGC TGGATATGAC CAACAATTGC 
CTGAAGGAGA TTCCCGCCTC GTTGCACGAA CTGAGCAGTC TGTCAGTGCT GAATATTAGT 
GGCAACGTCA ACATCACGGA ACTGCCGCCG CACTTGGGAC TGCTGTCGAG ACTTTGGAAT 
CTTAATACGC GCGGTTGCCT ACTGCAGGAG CCACTGCGCT CTATGATCGA GAGCAAGAAG 
CACAAGACGA TGGACATTGT TGGATATCTT AAATCTATTT ACGAAGATGC TCAGCCCTAC 
GCACGGATGA AACTGATGGT GGTTGGTGTG GCTGGAATTG GCAAGAGCAC TCTGCTGGAC 
TTACTGCGCC AGGGGGCGGG CTCAGGGTCC AGCTCCAGCT CTCATCGATC GCGCGCCAGC 
GAAAATCATT GGGCCAAGCG AATGGGACAC GCACGCAGCA CATCTCGATC TCATCGCCAT 
TCGTCGGCTT CTAGCGCAAA CATTTCTACA GTAGGAGTGG ACATTGGAAC ATGGATATGT 
GAGAAACGAA AGCGAGCACC CGGCTCCCAC GGCCCAGTGG TGTTTCGTAC TTGGGATTTT 
GGTGGTCAGA AGGAGTACTA CGCCACACAT CAGTACTTTT TGTCCAAACG GAGTCTGTAT 
TTGGTGCTCT GGCGCATTAG CGACGGTCAC AAGGGTCTGG CGGAGTTGCT GCAGTGGCTT 
GGCAACATTC AAGCGAGGGC TCCCAACTCC CCCGTAATCA TTGTGGGCAC ACACTTTGAT 
GCCGTTGGTG AATCCATCTC GCCCCAAAAG GCAGAGCAAC TGCAGCAATT GATTCGGGAG 
AAGTTTATCG CCATTCCCGA TGCGGAAAAA ATTGGGCTGC CACGGGTGAT TGATTCCATC 
GAAATAAGTT GCCGGACCCT ACACAATATC CACTTATTGG CCAACATTAT TTACGACACC 
GCCATGCAAC TGCGATCCCC TGGTTCCAAG GAGCCCATGC TACTGCAAAA GATCCCCGCC 
AGCTACATTG CCTTGGAGGA TATTGTGAAT GTGATTGCAT GCAATCTGCG CGCTGCTGGG 
CGAGATCCCG TCCTGGATGG TGAACAATAT AAGCGCTTGG TCACCGAACA GATGAGATTG 
CACAACTACA AGAGCTTCCG GGATGCCGCT GAGCTGCAGC AGGCCACTAC ATGGTGCCAC 
GAAAATGGAG TTTTATTGCA CTATGATGAT GCTACGCTAA GGGACTACTA CTTTCTCGAT 
CCACAGTGGC TTTGCGACAT GCTGGCCCAT GTGGTCACCG TGCGGGAGAT CAACCCCTTT 
GCTCCTACGG GCGTGATGAA GCTGGATGAT CTCCAGATGT TGTTCCGAAG TGTGCAGGTT 
CAAGGAAATG GAAACAGAAG CTACATTGTC AGTCTACTTA ACAAGTTTGA GGTTGCTTTA 
ACGTGGGATT CGAGAACTCT ACTCATACCC TCCTTACTGC CTTCGCAGGA GGCAGCTACA 
CCCAATTCAG GAAGCACGGT TAAGCTATCG CAACGATCTC GCGGCCGTAG TTTGGGTTGC 
TCTGTATCGC AAGAAGTAAA TCTAAATAAT TTGATATACG AACAGAGGTC AGCTCCTTCT 
TCATCATCTT CCTCTGCATC GGTTAGCCAA GGCTTGCGGC GCATCCTATT GATGACTTAT 
TTCCCGTCCG GGTTTTGGTC AAGATTAATC ACCCGGATTT TAGCTGATGA ACAAATAATC 
GAAGCAATTC GTGGCGTTTA TATGGCTTCA CAAGATTACG CGGACTTTGA TCTACGCACA 
TCGTTGGAGC AGGACACCCA ATGGAATCTT TGGCAGACAG GCTTAGCACT GTACTATGGA 
CCTATCTTAA TATTCAAGAT CTGGGAAGTA CCCTTTCAGA AGACAGAGCG AACGCAACCT 
TTCCGCACTG ATGGCAACCG ATTTAAGCTG AAACAAGATG GTATCTGGAG CGATGTAAAT 
CTCAGCTCCT CTAGCATTTT GGAAGTTTAC TTTCCACTCT ATGAAGTGAA CATATCGCAA 
GAAGTGGATG ACAATGAACG TCAATTGCTG GCCGAGATTC GTCCGCACAT GTCTCAGGTG 
GCCAAGCTTT TGGCCCTAAC AGTGGATCAT ATTGACTTGC TTTTGGAGGA TTGGTATCCT 
TCATTGGGAA CGCGGTTTGT GCACACTTCC GAGGGTCGAT TCCTAATCAC TCGATTGGTG 
TTGTGTCCGC GATGTCTTTG GAAGTTGCAG CTGCAGCAGA ACAACGAACC GTCTGATCGG 
GAAGTGCCTC CAGTGGGTTG CAATCGACCA AGTCGAAGTA GTAGGCGTGG AGCGGGAGCA 
TACTTTCTCC ACGGTGTAGG TGATCCCGGA GAGGATGGTG CTTTAAACGT GTTCTCGGCC 
TATTTAAACG CCACAGCAAG GAGAGAACGC CGATCGGAGG ATTCTTTGGG TGCTGGATCC 
GACGCGGACT CGGGCGTGGG TCCCGACTCA GCCGGTTCTT CGCGCAACAC TTCAGTTGAT 
GGTCATCCCG GATATCACTT GCCTGATAAC TCAAATGTGT GTTATGCTTG GATGATTGAG 
GAGTGCATTT TGTCCGTTTA TAATCAAAGC AAGATTAGTT GCCCTGTTCA TCTTGAACAG 
TCTATGGCTC AGCTTGCGCC AGACGTAATC TTTGCCGATA TTCCTGATAA GCACACTATT 
CCAAGCGAGT GCATCATCAA GGGATCGTTG CTTGGTCGAG GTGCTTTTGG GTTCGTTTTT 
AAAGCAAATT GCAAGGTAAG GGGCGCCAGA TCCTTTAAGC CGGTGGCAAT GAAAATGCTT 
CAGCCAGTGC CTCCCGGAGC TCGTGCAAAA GAGAGCGCTT TGATGGCTTT CAAGGTAGCT 
GTAGGCAAAT GGGACCGGGA TCCACTGCAG CACTCTTGCA AGGCCTACTG CACTGCGCGT 
CAGGAATTGG CAGTACTTCT AACTCTCAAG CATCCAAACA TTGTACCCTT GGTCGGGATC 
TGCATTAAAC CATTAGCTCT GGTTCTGGAA CTAGCTCCTC TAGGCGGTTT GGACGCTTTG 
CTCCGGCATT ACCGACGCAG CGGAGCCCAC ATGGGACCGC ATACTTTCCA GACCCTTGTC 
CTGCAGGCGG CTCGAGCAAT CGAGTATTTG CATCGCAGAA GAATCATCTA CCGCGATCTA 
AAGTCTGAAA ATGTCTTGGT TTGGGAGCTT CCACAACCAC ACACGGAAGA CAGTCCCCGC 
AACCTTGTGC ACATAAAGAT TGCCGACTAC GGAATTAGCC GGCAAACTGC TCCAAGTGGA 
GCTAAAGGAT TTGGTGGCAC CGAGGGTTTT ATGGCTCCGG AGATCATACG CTACAATGGT 
GAGGAGGAAT ATACTGAAAA GGTGGACTGC TTCTCATTTG GAATGTTCAT ATATGAGAAT 
ATCAGTTTAC GACAACCTTT CGAAGGCCAC GAGTCTATTA AAGAGTGCAT CTTGGAGGGT 
AGTCGCCCGG CTTTGACTCA AAGGGAAACC CAGTTTCCCA CCTGCTGTTT GGATCTTATG 
GTCTTGTGTT GGCACGAACA GCCTCGTCGC AGACCGACGG CAAGTCAGAT TGTTTCCATA 
CTAAGTGCAC CGGAGTGCAT CCACCTCCTG GATGTCGTTG CCATGCCGCA TAGCGAGAAG 
ATTGTCTGTG GGGTTTTTCA GTCGCTTGTT GGTATGGGCG ATGATGAACG ATGTGGTCTT 
GAACTGTGGC TACCCTCCTT CGGCTCTCGC ATTGACATTC TAGACTGCTC ACCCTCGGGC 
AGCCTACTGC AGTGCAACAG CATCAGTTGT TCTCCGCAGC CACAGGTTGC TCCGCCCAAG 
ACACCCGAAA ACGGTGCAAA TTCACGTGCC CGCTCTGCCC AACGTTTGCC CAAGATGAAC 
ATGTTGTGCT GCTGCCTGGT GGGTGAAGCC ATCTGGATGG GCGACGTTTC CGGCAATCTG 
CACGCCTATA GCACATCCAC CTATGCCCAC CTGTTTTCCT ACATGCTCGA TCCCAACATT 
AAGTCAGCTG TGATCAGTCT AGTCTACATG GAGAAAATAG CTCGCGTGGC TGTCGGAACG 
CATAACGGTC GGGTGTTCCT TGTTGACGCC ACGCAGATGC CCAGCAATTG CGCATTTGCC 
GAGGGCTCTT TTGTGCTTAC AGAGATCTGT TCTGGTTTTG TGTTGCACGC CGCTTGTTCC 
GTTGTTGTGG ATGGAATCTA TGAATTGTGG TGCGGCGAAA TTGCAGGAAA GATAAATGTT 
TTTCCGTTGA ACGAGAATGG AGTTTCCGGA CATCAGGCTT TATGCCACAG CGAGGAACCC 
AATCTCATCG AGGACGTCAA GGTGGCTCGA ATGTGCAGTA ATGAAAGCCA TGTGTTCAGT 
TGTCTATATC CCGGTTGTAT GGTGTACCAG TGGGATGTTA TCTCCAAGCG GATAGAAAAT 
AAGCTGGACT GCTCAAAGCT GCTGCCCTGC TCCGAGTCTC TGCAAAGTAT TGCGATTGAT 
GAGCATGTGA ACCTTATAAA ATGCCAGATT TCGGCACTGG CGGCTCACAA TTCAGAACTG 
TATATTGGGA CGACCTGGGG CTGCTTAATT GTGGCCGAAC TGCACACCCT GCGCCCCATT 
AGTGTTTTCC GACCCTATGA AAATGAGATC AAATCTATAA TAACCCTTTC TAAAGACAAT 
GTTCCGCTGA TCGCCACGAT CGGAAGACGA TATCGCTCTC TGATCTCTCG ATATGTGGAC 
TCCGCAGAGT CATCCACCAA GAGCTCTGCC GTCAGCACAC CCACCCATGG TGCAGCCAAA 
TCCGTGCCAC CGGCGGATGT GGATAATCAC ATTCATTGCC TGCTGTGGCG CGCCAAGCAC 
TGGACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262771.1
CDS	159..7427
Translation	MSDSDEDAREEVRQIKHGELRTAVISGDERTVRVLLAALGTERQIIVNMAPSGANTLLFLACQSGYESITQRLLDAGADGRSHAVTKYSPLYAAVHSGHLGIARLMLDHFPELIQQPTVERWLPLHAACINGHIKLLELLISYSYPDYLYQTYRDEEGQWEWRLPFDANAHDVTGQTSLYIASILGNKQLVGVLLKWQLHCRRTLGDSASSVSTPITPTRKRISFGIQAIMSKLHISGESEGPDDLASQESTECQRCPINVNLLCGAARETALLAAVRGGHLDVVQSLLQHGANPNIVAKPVEDHNDPKCCEEIYGLSNVPIAEACKQRSLAMLDLLLKHGARDDNGTAIGMAITCGDEAILSRLLARRVHPDSDYKINKKGLPTPVEVNVFLPSTSNISYSAMFPNNPTIIDWHSMGSSVQLSVVRVPWMVSGVLLLNPKLQSHPRLNEVALTAITRIDFSHNVLTSIPQELFHLVSLRYLNVAQNKITDLPAPIGQTYGCPVLDELFLQDNQLTTLPAAIFHLPALSILDVSNNKLQQLPFDLWRAPKLRELNVAFNLLRDLPVPPMQTSSSLLSLDKLNLQSFEEPPSNKPRNVTQQRLTHRNLWSATLDITDNDMKWQHEQDLGDGKTAGVGSSQLSSLNIANNLFTSIPAALPCLAVNLTRLNMSYNSLRSMGHVTSYPATLKQLDLSHNEISCWPSLPRITESDPHLLCYSCVQLPEGRDDDYKTASSKGSSSSATSFRASVLKSVCRHRRHLRLEALRTLILADNLLTRIQLSTDDATTLFNESEDADWSVVGVNRSKVIFPNLSMLDMTNNCLKEIPASLHELSSLSVLNISGNVNITELPPHLGLLSRLWNLNTRGCLLQEPLRSMIESKKHKTMDIVGYLKSIYEDAQPYARMKLMVVGVAGIGKSTLLDLLRQGAGSGSSSSSHRSRASENHWAKRMGHARSTSRSHRHSSASSANISTVGVDIGTWICEKRKRAPGSHGPVVFRTWDFGGQKEYYATHQYFLSKRSLYLVLWRISDGHKGLAELLQWLGNIQARAPNSPVIIVGTHFDAVGESISPQKAEQLQQLIREKFIAIPDAEKIGLPRVIDSIEISCRTLHNIHLLANIIYDTAMQLRSPGSKEPMLLQKIPASYIALEDIVNVIACNLRAAGRDPVLDGEQYKRLVTEQMRLHNYKSFRDAAELQQATTWCHENGVLLHYDDATLRDYYFLDPQWLCDMLAHVVTVREINPFAPTGVMKLDDLQMLFRSVQVQGNGNRSYIVSLLNKFEVALTWDSRTLLIPSLLPSQEAATPNSGSTVKLSQRSRGRSLGCSVSQEVNLNNLIYEQRSAPSSSSSSASVSQGLRRILLMTYFPSGFWSRLITRILADEQIIEAIRGVYMASQDYADFDLRTSLEQDTQWNLWQTGLALYYGPILIFKIWEVPFQKTERTQPFRTDGNRFKLKQDGIWSDVNLSSSSILEVYFPLYEVNISQEVDDNERQLLAEIRPHMSQVAKLLALTVDHIDLLLEDWYPSLGTRFVHTSEGRFLITRLVLCPRCLWKLQLQQNNEPSDREVPPVGCNRPSRSSRRGAGAYFLHGVGDPGEDGALNVFSAYLNATARRERRSEDSLGAGSDADSGVGPDSAGSSRNTSVDGHPGYHLPDNSNVCYAWMIEECILSVYNQSKISCPVHLEQSMAQLAPDVIFADIPDKHTIPSECIIKGSLLGRGAFGFVFKANCKVRGARSFKPVAMKMLQPVPPGARAKESALMAFKVAVGKWDRDPLQHSCKAYCTARQELAVLLTLKHPNIVPLVGICIKPLALVLELAPLGGLDALLRHYRRSGAHMGPHTFQTLVLQAARAIEYLHRRRIIYRDLKSENVLVWELPQPHTEDSPRNLVHIKIADYGISRQTAPSGAKGFGGTEGFMAPEIIRYNGEEEYTEKVDCFSFGMFIYENISLRQPFEGHESIKECILEGSRPALTQRETQFPTCCLDLMVLCWHEQPRRRPTASQIVSILSAPECIHLLDVVAMPHSEKIVCGVFQSLVGMGDDERCGLELWLPSFGSRIDILDCSPSGSLLQCNSISCSPQPQVAPPKTPENGANSRARSAQRLPKMNMLCCCLVGEAIWMGDVSGNLHAYSTSTYAHLFSYMLDPNIKSAVISLVYMEKIARVAVGTHNGRVFLVDATQMPSNCAFAEGSFVLTEICSGFVLHAACSVVVDGIYELWCGEIAGKINVFPLNENGVSGHQALCHSEEPNLIEDVKVARMCSNESHVFSCLYPGCMVYQWDVISKRIENKLDCSKLLPCSESLQSIAIDEHVNLIKCQISALAAHNSELYIGTTWGCLIVAELHTLRPISVFRPYENEIKSIITLSKDNVPLIATIGRRYRSLISRYVDSAESSTKSSAVSTPTHGAAKSVPPADVDNHIHCLLWRAKHWT

Target ORF information:

RefSeq Version	NM_001275842.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Leucine-rich repeat kinase (Lrrk), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275842.1

ORF Insert Sequence:

ATGAGCGACT CCGACGAAGA TGCCCGCGAG GAAGTGCGTC AGATCAAGCA CGGGGAGCTG 
CGAACGGCAG TGATCTCTGG CGATGAGCGG ACTGTGCGGG TGCTGCTGGC AGCTCTTGGA 
ACTGAGCGTC AGATTATAGT CAATATGGCT CCTTCAGGAG CAAACACCCT TCTATTCCTA 
GCCTGCCAGT CTGGTTACGA GAGCATTACA CAGCGATTGC TGGATGCTGG AGCGGATGGT 
CGCTCCCATG CCGTGACCAA GTACTCACCC TTGTACGCCG CCGTCCATAG CGGTCACTTG 
GGCATTGCCC GACTGATGCT AGACCATTTT CCAGAACTGA TCCAGCAGCC GACTGTAGAG 
CGCTGGCTGC CGCTGCACGC CGCCTGCATC AATGGACACA TCAAGCTGCT GGAACTCCTT 
ATCAGCTACA GTTATCCCGA CTACCTCTAC CAGACATATC GCGACGAGGA GGGCCAGTGG 
GAATGGCGGC TCCCCTTCGA CGCTAACGCT CATGATGTGA CGGGTCAGAC GAGTCTGTAT 
ATTGCCAGCA TCCTAGGAAA CAAGCAGCTG GTTGGTGTGC TTCTAAAGTG GCAGCTCCAT 
TGCCGGCGTA CGTTGGGCGA TTCCGCCAGC TCGGTGAGCA CTCCTATTAC GCCCACCAGG 
AAACGTATTT CATTTGGCAT TCAGGCTATC ATGTCCAAAC TGCACATATC TGGAGAGTCA 
GAAGGACCCG ACGACCTAGC TTCACAAGAG TCAACCGAGT GCCAACGGTG TCCCATTAAC 
GTGAATCTGC TATGTGGAGC GGCGAGAGAA ACAGCTCTGC TTGCGGCCGT TCGAGGCGGC 
CACCTAGACG TGGTGCAGTC TCTGCTACAG CACGGCGCAA ATCCGAATAT TGTAGCCAAG 
CCAGTTGAGG ATCACAACGA CCCGAAATGT TGCGAGGAAA TATATGGGCT CAGTAATGTC 
CCCATTGCAG AGGCCTGCAA GCAAAGGTCG CTGGCTATGT TGGATCTCTT GCTTAAGCAT 
GGAGCCCGCG ACGATAATGG CACGGCCATT GGAATGGCTA TTACATGCGG CGACGAGGCC 
ATCCTGAGTC GTCTTTTGGC CCGACGAGTC CATCCGGACT CAGACTACAA GATCAACAAG 
AAGGGTCTTC CCACACCAGT GGAGGTGAAC GTGTTTTTGC CGTCCACCAG CAACATATCT 
TACAGTGCTA TGTTCCCCAA CAATCCAACC ATTATTGACT GGCACAGCAT GGGCTCCAGT 
GTCCAGTTGT CTGTTGTAAG GGTTCCCTGG ATGGTCAGCG GTGTGTTGCT GCTGAATCCT 
AAGCTACAGT CGCATCCGCG ACTTAATGAG GTTGCTCTGA CAGCTATCAC GCGAATAGAT 
TTCTCGCACA ACGTTCTCAC GTCAATTCCA CAGGAGCTTT TCCATTTGGT TAGCCTAAGG 
TATTTAAATG TGGCGCAAAA CAAGATAACC GATCTACCTG CACCAATAGG CCAGACATAT 
GGGTGTCCGG TACTGGATGA ACTCTTTCTA CAAGACAACC AGTTAACTAC GCTGCCAGCC 
GCCATCTTTC ACCTGCCCGC CCTATCTATA TTGGATGTTT CAAATAACAA ACTGCAACAG 
CTTCCCTTCG ACCTGTGGCG TGCACCCAAG TTGCGCGAAC TTAATGTGGC CTTCAACCTA 
TTGCGGGATC TTCCTGTGCC GCCAATGCAA ACTAGCAGTT CGCTGCTGAG CCTAGACAAA 
TTGAATCTGC AATCTTTTGA GGAGCCGCCA TCAAACAAAC CGCGAAATGT GACTCAGCAA 
CGTCTCACCC ATCGCAATCT ATGGTCGGCT ACTTTGGACA TAACCGATAA TGACATGAAG 
TGGCAGCATG AACAAGATTT AGGCGATGGA AAGACAGCTG GTGTGGGCTC CTCCCAGCTA 
AGTAGCTTAA ATATAGCAAA TAACCTCTTC ACCAGTATCC CCGCTGCTTT GCCCTGTCTG 
GCTGTAAACC TAACACGGCT GAATATGTCA TACAACAGTC TGCGTTCTAT GGGACATGTA 
ACCAGTTACC CGGCAACACT TAAGCAGTTG GATCTAAGTC ACAACGAGAT ATCCTGCTGG 
CCAAGTTTAC CGCGAATTAC TGAATCGGAT CCGCATCTAC TGTGCTACAG TTGTGTCCAG 
CTACCAGAGG GTCGAGATGA CGACTACAAG ACGGCCTCCT CAAAGGGCAG TAGCTCGTCT 
GCCACATCGT TTCGCGCTTC GGTGTTAAAA AGTGTTTGTC GGCACAGGCG TCATCTGCGC 
CTGGAGGCTC TACGCACATT GATCTTGGCT GACAATCTGC TTACGCGCAT CCAACTATCA 
ACTGATGACG CCACAACCCT TTTCAATGAA AGTGAGGACG CCGATTGGAG TGTGGTAGGC 
GTGAACCGGT CTAAGGTGAT ATTTCCCAAC CTGTCTATGC TGGATATGAC CAACAATTGC 
CTGAAGGAGA TTCCCGCCTC GTTGCACGAA CTGAGCAGTC TGTCAGTGCT GAATATTAGT 
GGCAACGTCA ACATCACGGA ACTGCCGCCG CACTTGGGAC TGCTGTCGAG ACTTTGGAAT 
CTTAATACGC GCGGTTGCCT ACTGCAGGAG CCACTGCGCT CTATGATCGA GAGCAAGAAG 
CACAAGACGA TGGACATTGT TGGATATCTT AAATCTATTT ACGAAGATGC TCAGCCCTAC 
GCACGGATGA AACTGATGGT GGTTGGTGTG GCTGGAATTG GCAAGAGCAC TCTGCTGGAC 
TTACTGCGCC AGGGGGCGGG CTCAGGGTCC AGCTCCAGCT CTCATCGATC GCGCGCCAGC 
GAAAATCATT GGGCCAAGCG AATGGGACAC GCACGCAGCA CATCTCGATC TCATCGCCAT 
TCGTCGGCTT CTAGCGCAAA CATTTCTACA GTAGGAGTGG ACATTGGAAC ATGGATATGT 
GAGAAACGAA AGCGAGCACC CGGCTCCCAC GGCCCAGTGG TGTTTCGTAC TTGGGATTTT 
GGTGGTCAGA AGGAGTACTA CGCCACACAT CAGTACTTTT TGTCCAAACG GAGTCTGTAT 
TTGGTGCTCT GGCGCATTAG CGACGGTCAC AAGGGTCTGG CGGAGTTGCT GCAGTGGCTT 
GGCAACATTC AAGCGAGGGC TCCCAACTCC CCCGTAATCA TTGTGGGCAC ACACTTTGAT 
GCCGTTGGTG AATCCATCTC GCCCCAAAAG GCAGAGCAAC TGCAGCAATT GATTCGGGAG 
AAGTTTATCG CCATTCCCGA TGCGGAAAAA ATTGGGCTGC CACGGGTGAT TGATTCCATC 
GAAATAAGTT GCCGGACCCT ACACAATATC CACTTATTGG CCAACATTAT TTACGACACC 
GCCATGCAAC TGCGATCCCC TGGTTCCAAG GAGCCCATGC TACTGCAAAA GATCCCCGCC 
AGCTACATTG CCTTGGAGGA TATTGTGAAT GTGATTGCAT GCAATCTGCG CGCTGCTGGG 
CGAGATCCCG TCCTGGATGG TGAACAATAT AAGCGCTTGG TCACCGAACA GATGAGATTG 
CACAACTACA AGAGCTTCCG GGATGCCGCT GAGCTGCAGC AGGCCACTAC ATGGTGCCAC 
GAAAATGGAG TTTTATTGCA CTATGATGAT GCTACGCTAA GGGACTACTA CTTTCTCGAT 
CCACAGTGGC TTTGCGACAT GCTGGCCCAT GTGGTCACCG TGCGGGAGAT CAACCCCTTT 
GCTCCTACGG GCGTGATGAA GCTGGATGAT CTCCAGATGT TGTTCCGAAG TGTGCAGGTT 
CAAGGAAATG GAAACAGAAG CTACATTGTC AGTCTACTTA ACAAGTTTGA GGTTGCTTTA 
ACGTGGGATT CGAGAACTCT ACTCATACCC TCCTTACTGC CTTCGCAGGA GGCAGCTACA 
CCCAATTCAG GAAGCACGGT TAAGCTATCG CAACGATCTC GCGGCCGTAG TTTGGGTTGC 
TCTGTATCGC AAGAAGTAAA TCTAAATAAT TTGATATACG AACAGAGGTC AGCTCCTTCT 
TCATCATCTT CCTCTGCATC GGTTAGCCAA GGCTTGCGGC GCATCCTATT GATGACTTAT 
TTCCCGTCCG GGTTTTGGTC AAGATTAATC ACCCGGATTT TAGCTGATGA ACAAATAATC 
GAAGCAATTC GTGGCGTTTA TATGGCTTCA CAAGATTACG CGGACTTTGA TCTACGCACA 
TCGTTGGAGC AGGACACCCA ATGGAATCTT TGGCAGACAG GCTTAGCACT GTACTATGGA 
CCTATCTTAA TATTCAAGAT CTGGGAAGTA CCCTTTCAGA AGACAGAGCG AACGCAACCT 
TTCCGCACTG ATGGCAACCG ATTTAAGCTG AAACAAGATG GTATCTGGAG CGATGTAAAT 
CTCAGCTCCT CTAGCATTTT GGAAGTTTAC TTTCCACTCT ATGAAGTGAA CATATCGCAA 
GAAGTGGATG ACAATGAACG TCAATTGCTG GCCGAGATTC GTCCGCACAT GTCTCAGGTG 
GCCAAGCTTT TGGCCCTAAC AGTGGATCAT ATTGACTTGC TTTTGGAGGA TTGGTATCCT 
TCATTGGGAA CGCGGTTTGT GCACACTTCC GAGGGTCGAT TCCTAATCAC TCGATTGGTG 
TTGTGTCCGC GATGTCTTTG GAAGTTGCAG CTGCAGCAGA ACAACGAACC GTCTGATCGG 
GAAGTGCCTC CAGTGGGTTG CAATCGACCA AGTCGAAGTA GTAGGCGTGG AGCGGGAGCA 
TACTTTCTCC ACGGTGTAGG TGATCCCGGA GAGGATGGTG CTTTAAACGT GTTCTCGGCC 
TATTTAAACG CCACAGCAAG GAGAGAACGC CGATCGGAGG ATTCTTTGGG TGCTGGATCC 
GACGCGGACT CGGGCGTGGG TCCCGACTCA GCCGGTTCTT CGCGCAACAC TTCAGTTGAT 
GGTCATCCCG GATATCACTT GCCTGATAAC TCAAATGTGT GTTATGCTTG GATGATTGAG 
GAGTGCATTT TGTCCGTTTA TAATCAAAGC AAGATTAGTT GCCCTGTTCA TCTTGAACAG 
TCTATGGCTC AGCTTGCGCC AGACGTAATC TTTGCCGATA TTCCTGATAA GCACACTATT 
CCAAGCGAGT GCATCATCAA GGGATCGTTG CTTGGTCGAG GTGCTTTTGG GTTCGTTTTT 
AAAGCAAATT GCAAGGTAAG GGGCGCCAGA TCCTTTAAGC CGGTGGCAAT GAAAATGCTT 
CAGCCAGTGC CTCCCGGAGC TCGTGCAAAA GAGAGCGCTT TGATGGCTTT CAAGGTAGCT 
GTAGGCAAAT GGGACCGGGA TCCACTGCAG CACTCTTGCA AGGCCTACTG CACTGCGCGT 
CAGGAATTGG CAGTACTTCT AACTCTCAAG CATCCAAACA TTGTACCCTT GGTCGGGATC 
TGCATTAAAC CATTAGCTCT GGTTCTGGAA CTAGCTCCTC TAGGCGGTTT GGACGCTTTG 
CTCCGGCATT ACCGACGCAG CGGAGCCCAC ATGGGACCGC ATACTTTCCA GACCCTTGTC 
CTGCAGGCGG CTCGAGCAAT CGAGTATTTG CATCGCAGAA GAATCATCTA CCGCGATCTA 
AAGTCTGAAA ATGTCTTGGT TTGGGAGCTT CCACAACCAC ACACGGAAGA CAGTCCCCGC 
AACCTTGTGC ACATAAAGAT TGCCGACTAC GGAATTAGCC GGCAAACTGC TCCAAGTGGA 
GCTAAAGGAT TTGGTGGCAC CGAGGGTTTT ATGGCTCCGG AGATCATACG CTACAATGGT 
GAGGAGGAAT ATACTGAAAA GGTGGACTGC TTCTCATTTG GAATGTTCAT ATATGAGAAT 
ATCAGTTTAC GACAACCTTT CGAAGGCCAC GAGTCTATTA AAGAGTGCAT CTTGGAGGGT 
AGTCGCCCGG CTTTGACTCA AAGGGAAACC CAGTTTCCCA CCTGCTGTTT GGATCTTATG 
GTCTTGTGTT GGCACGAACA GCCTCGTCGC AGACCGACGG CAAGTCAGAT TGTTTCCATA 
CTAAGTGCAC CGGAGTGCAT CCACCTCCTG GATGTCGTTG CCATGCCGCA TAGCGAGAAG 
ATTGTCTGTG GGGTTTTTCA GTCGCTTGTT GGTATGGGCG ATGATGAACG ATGTGGTCTT 
GAACTGTGGC TACCCTCCTT CGGCTCTCGC ATTGACATTC TAGACTGCTC ACCCTCGGGC 
AGCCTACTGC AGTGCAACAG CATCAGTTGT TCTCCGCAGC CACAGGTTGC TCCGCCCAAG 
ACACCCGAAA ACGGTGCAAA TTCACGTGCC CGCTCTGCCC AACGTTTGCC CAAGATGAAC 
ATGTTGTGCT GCTGCCTGGT GGGTGAAGCC ATCTGGATGG GCGACGTTTC CGGCAATCTG 
CACGCCTATA GCACATCCAC CTATGCCCAC CTGTTTTCCT ACATGCTCGA TCCCAACATT 
AAGTCAGCTG TGATCAGTCT AGTCTACATG GAGAAAATAG CTCGCGTGGC TGTCGGAACG 
CATAACGGTC GGGTGTTCCT TGTTGACGCC ACGCAGATGC CCAGCAATTG CGCATTTGCC 
GAGGGCTCTT TTGTGCTTAC AGAGATCTGT TCTGGTTTTG TGTTGCACGC CGCTTGTTCC 
GTTGTTGTGG ATGGAATCTA TGAATTGTGG TGCGGCGAAA TTGCAGGAAA GATAAATGTT 
TTTCCGTTGA ACGAGAATGG AGTTTCCGGA CATCAGGCTT TATGCCACAG CGAGGAACCC 
AATCTCATCG AGGACGTCAA GGTGGCTCGA ATGTGCAGTA ATGAAAGCCA TGTGTTCAGT 
TGTCTATATC CCGGTTGTAT GGTGTACCAG TGGGATGTTA TCTCCAAGCG GATAGAAAAT 
AAGCTGGACT GCTCAAAGCT GCTGCCCTGC TCCGAGTCTC TGCAAAGTAT TGCGATTGAT 
GAGCATGTGA ACCTTATAAA ATGCCAGATT TCGGCACTGG CGGCTCACAA TTCAGAACTG 
TATATTGGGA CGACCTGGGG CTGCTTAATT GTGGCCGAAC TGCACACCCT GCGCCCCATT 
AGTGTTTTCC GACCCTATGA AAATGAGATC AAATCTATAA TAACCCTTTC TAAAGACAAT 
GTTCCGCTGA TCGCCACGAT CGGAAGACGA TATCGCTCTC TGATCTCTCG ATATGTGGAC 
TCCGCAGAGT CATCCACCAA GAGCTCTGCC GTCAGCACAC CCACCCATGG TGCAGCCAAA 
TCCGTGCCAC CGGCGGATGT GGATAATCAC ATTCATTGCC TGCTGTGGCG CGCCAAGCAC 
TGGACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Lrrk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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