RASSF8 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	RASSF8
Entrez Gene ID	43096
Full Name	Ras association domain family member 8
Synonyms	Boa,CG5053,Dmel\CG5053,dRASSF8,dmRASSF7/8
General protein information	Preferred Names Ras association domain family member 8 Names CG5053 gene product from transcript CG5053-RA CG5053-PA RASSF8-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 96E8-96E8

mRNA and Protein(s)

mRNA	Protein	Name
NM_143154.4	NP_651411.1	Ras association domain family member 8

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	RASSF8	NP_651411.1
Danio rerio (zebrafish)	LOC100003291	XP_001342892.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Drosophila MAGI interacts with RASSF8 to regulate E-Cadherin-based adherens junctions in the developing eye.
Title: Drosophila MAGI interacts with RASSF8 to regulate E-Cadherin-based adherens junctions in the developing eye.

The following RASSF8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASSF8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06114	NM_143154.4 Latest version!	Drosophila melanogaster Ras association domain family member 8 (RASSF8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RASSF8 ( NM_143154.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651411.1 Drosophila melanogaster Ras association domain family member 8 (RASSF8), mRNA.

Price & Availability↑

CloneID	OFa06114
Clone ID Related Accession (Same CDS sequence)	NM_143154.4
Accession Version	NM_143154.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ras association domain family member 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_143154.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCTGA AAGTTTGGGT CGAGGGCATC CAGCGGATAG TTTGCGGCGT TACAGTGAAC 
ACCACATGTC AGGATGTGGT TTTTGCTTTG GCCCATGCCA CTGGAAAAGT GGGCAGATTC 
ACTTTAATCG AAAGGTGGCG CAATAACGAA CGCCACCTGG CCCCCAATGA GAATCCAATG 
AAGCTGCTGC TCAAATGGGG CGAGTATGCA AATGATGTGC AGTTTATATT ACAACGTTCG 
GAAAACAAAA CACAGCAGCA GCAGCAGCAA CAACAACAGC AGCAGCAGAA CAATAATAAT 
AATAACAACT ATGCTGTGAT GACAACAACA AAAAAGCCGC TGGGGCCGAA CAACAATAAC 
AATAATAAGC CAGCGCCCAC TTTGCTAAAG CAAAAGTCGA ACGTCGAACT CAGCTACCGC 
ACAAAGGAAT TGAAAAAAAG TTTCGGGGGC CACGATGCCA AGCAGTTTGA CAACATTGGA 
ATCGTCAAGG GAATTCCGCA GCAGCAGCAA TTGCAGTCAC AACAAACGGC CATGGGAGCA 
CCGGCGAGTC CACCACAGGA GCAGCTGACA CCGCAGGATA ACAATAATGT AGCACCACTG 
GTGCCAAAGC ATGAGAAATC GCTGTCCAAT CCGCTGGACA TGACCAGCAC GGGTAGCAAT 
CCACCCGCAC CTACAAATGG CTACAATGAC TTCTACAACA ATAACAGCAG CAGTAATCAG 
CATATATACA GCCAGCTGAG CAAATCCAGC AATGAACTCC GACGCTCCAG TCCCAACGAA 
ATGTACTACA ACAATAATAA CAATATTGTT AATAACAATA ACAACTCACC GGAGCTGTAC 
AAGCAAACGC CGACGATTTC CGCGAATGGA GCTCTAGTCC CACCACCATA TCGTGACCCG 
CCACCCCCGA GAAACAGCCC CATGTGCCAG GGGCAGCGTT TGGCCAGCAG CAATGCGGAC 
ACCATGTCCA ATGCCTCCTC CTCGACGAAT CCGTTTCTCA GCGACTACGA TCAGCATAGC 
AGCCACAACA CTAATAGCAT CAACAATAAT AGCCAGGCCA TGGAGGAGGG TGAGCCCATG 
TTCCAGACCA CGCAGTATAA CGATTTGCTG CAGCTCATCA AGTTTCAGCG GGAGAAGATC 
ACAGCCCAGC AAATGGAATT GCAAAAGTTC GATACGGAAA TAGTTTATTT GGAGAGCAAA 
GAACGCGACC ACGCCCACGA GTTGGATGCT ATTTCGCGTG AGATTTCCAA GGCGGATCAA 
ATCTTTCGGC AAGGAAGCGA ACAGCTCCAG ACTCTGCAGT ATGTGGAAGA GGAAAACGAA 
TTGGTCAAGC AGCAAGAGAA GACTTTGAAA TCAGAAATAG CTCTACTGCG ATCCAAGCTG 
GCCAATTGCG AAACAGAGCT GCTACAATGC AAGAACAAAA TGCGCTTGCT GATGGATGAC 
ATTGAGCTGG AGCAGCAACA TCAACAGCAG ACCAATAGGC AAACTGTGGA GCGAGACTTC 
TTGATGGAAA TGGAGCGCAT TCAGAGTGAA ATCGACCAGG CTCTGCACAA CACTGACACT 
TCAAACAAAA CTGCAGATAG TTTAAAGAAG GAACTACTCA TGATCGAGCA TGCCATCGCG 
GACAAGAAGC GGCAGGTGGA GCAGCTGGTC AACGAGATGA AGGAGGTGAA TTTGCAGAGC 
CTGACTGTGA CCACCACGGA GGAGATCAAG CATCTATTGG AAGGACCCAA CAAGCAGGGC 
AGCAGCCGGC GGATCATCGG TTCGCCCAGG CAACTTGAAA CGGCGGTTCC AACCAGTAAA 
AATCCTCATG GTGTGTGGGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651411.1
CDS	621..2444
Translation	MELKVWVEGIQRIVCGVTVNTTCQDVVFALAHATGKVGRFTLIERWRNNERHLAPNENPMKLLLKWGEYANDVQFILQRSENKTQQQQQQQQQQQQNNNNNNNYAVMTTTKKPLGPNNNNNNKPAPTLLKQKSNVELSYRTKELKKSFGGHDAKQFDNIGIVKGIPQQQQLQSQQTAMGAPASPPQEQLTPQDNNNVAPLVPKHEKSLSNPLDMTSTGSNPPAPTNGYNDFYNNNSSSNQHIYSQLSKSSNELRRSSPNEMYYNNNNNIVNNNNNSPELYKQTPTISANGALVPPPYRDPPPPRNSPMCQGQRLASSNADTMSNASSSTNPFLSDYDQHSSHNTNSINNNSQAMEEGEPMFQTTQYNDLLQLIKFQREKITAQQMELQKFDTEIVYLESKERDHAHELDAISREISKADQIFRQGSEQLQTLQYVEEENELVKQQEKTLKSEIALLRSKLANCETELLQCKNKMRLLMDDIELEQQHQQQTNRQTVERDFLMEMERIQSEIDQALHNTDTSNKTADSLKKELLMIEHAIADKKRQVEQLVNEMKEVNLQSLTVTTTEEIKHLLEGPNKQGSSRRIIGSPRQLETAVPTSKNPHGVWV

Target ORF information:

RefSeq Version	NM_143154.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ras association domain family member 8 (RASSF8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143154.4

ORF Insert Sequence:

ATGGAGCTGA AAGTTTGGGT CGAGGGCATC CAGCGGATAG TTTGCGGCGT TACAGTGAAC 
ACCACATGTC AGGATGTGGT TTTTGCTTTG GCCCATGCCA CTGGAAAAGT GGGCAGATTC 
ACTTTAATCG AAAGGTGGCG CAATAACGAA CGCCACCTGG CCCCCAATGA GAATCCAATG 
AAGCTGCTGC TCAAATGGGG CGAGTATGCA AATGATGTGC AGTTTATATT ACAACGTTCG 
GAAAACAAAA CACAGCAGCA GCAGCAGCAA CAACAACAGC AGCAGCAGAA CAATAATAAT 
AATAACAACT ATGCTGTGAT GACAACAACA AAAAAGCCGC TGGGGCCGAA CAACAATAAC 
AATAATAAGC CAGCGCCCAC TTTGCTAAAG CAAAAGTCGA ACGTCGAACT CAGCTACCGC 
ACAAAGGAAT TGAAAAAAAG TTTCGGGGGC CACGATGCCA AGCAGTTTGA CAACATTGGA 
ATCGTCAAGG GAATTCCGCA GCAGCAGCAA TTGCAGTCAC AACAAACGGC CATGGGAGCA 
CCGGCGAGTC CACCACAGGA GCAGCTGACA CCGCAGGATA ACAATAATGT AGCACCACTG 
GTGCCAAAGC ATGAGAAATC GCTGTCCAAT CCGCTGGACA TGACCAGCAC GGGTAGCAAT 
CCACCCGCAC CTACAAATGG CTACAATGAC TTCTACAACA ATAACAGCAG CAGTAATCAG 
CATATATACA GCCAGCTGAG CAAATCCAGC AATGAACTCC GACGCTCCAG TCCCAACGAA 
ATGTACTACA ACAATAATAA CAATATTGTT AATAACAATA ACAACTCACC GGAGCTGTAC 
AAGCAAACGC CGACGATTTC CGCGAATGGA GCTCTAGTCC CACCACCATA TCGTGACCCG 
CCACCCCCGA GAAACAGCCC CATGTGCCAG GGGCAGCGTT TGGCCAGCAG CAATGCGGAC 
ACCATGTCCA ATGCCTCCTC CTCGACGAAT CCGTTTCTCA GCGACTACGA TCAGCATAGC 
AGCCACAACA CTAATAGCAT CAACAATAAT AGCCAGGCCA TGGAGGAGGG TGAGCCCATG 
TTCCAGACCA CGCAGTATAA CGATTTGCTG CAGCTCATCA AGTTTCAGCG GGAGAAGATC 
ACAGCCCAGC AAATGGAATT GCAAAAGTTC GATACGGAAA TAGTTTATTT GGAGAGCAAA 
GAACGCGACC ACGCCCACGA GTTGGATGCT ATTTCGCGTG AGATTTCCAA GGCGGATCAA 
ATCTTTCGGC AAGGAAGCGA ACAGCTCCAG ACTCTGCAGT ATGTGGAAGA GGAAAACGAA 
TTGGTCAAGC AGCAAGAGAA GACTTTGAAA TCAGAAATAG CTCTACTGCG ATCCAAGCTG 
GCCAATTGCG AAACAGAGCT GCTACAATGC AAGAACAAAA TGCGCTTGCT GATGGATGAC 
ATTGAGCTGG AGCAGCAACA TCAACAGCAG ACCAATAGGC AAACTGTGGA GCGAGACTTC 
TTGATGGAAA TGGAGCGCAT TCAGAGTGAA ATCGACCAGG CTCTGCACAA CACTGACACT 
TCAAACAAAA CTGCAGATAG TTTAAAGAAG GAACTACTCA TGATCGAGCA TGCCATCGCG 
GACAAGAAGC GGCAGGTGGA GCAGCTGGTC AACGAGATGA AGGAGGTGAA TTTGCAGAGC 
CTGACTGTGA CCACCACGGA GGAGATCAAG CATCTATTGG AAGGACCCAA CAAGCAGGGC 
AGCAGCCGGC GGATCATCGG TTCGCCCAGG CAACTTGAAA CGGCGGTTCC AACCAGTAAA 
AATCCTCATG GTGTGTGGGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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RASSF8 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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