NfI cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	NfI
Entrez Gene ID	43782
Full Name	Nuclear factor I
Synonyms	CG2380,Dmel\CG2380,NFI
General protein information	Preferred Names Nuclear factor I Names CG2380 gene product from transcript CG2380-RC CG2380-PB CG2380-PC NfI-PB NfI-PC nuclear factor I
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 4 Map Location: 102B1-102B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_205870.3	NP_995592.2	nuclear factor I, isoform B
NM_001272122.2	NP_001259051.1	nuclear factor I, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Evidence of conserved intron locations within DNA-binding domain of Drosophila, C. elegans and Ciona NFI genes
Title: The Nuclear Factor I (NFI) gene family in mammary gland development and function.

The initial description of the Drosophila NfI gene and conserved intron positions relative to the C. elegans nfi-1 gene and vertebrate NFI genes are discussed in this paper.
Title: Roles of the NFI/CTF gene family in transcription and development.

The following NfI gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NfI cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00150	NM_001272122.2 Latest version!	Drosophila melanogaster nuclear factor I, transcript variant C (NfI), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa00251	NM_205870.3 Latest version!	Drosophila melanogaster nuclear factor I, transcript variant B (NfI), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NfI ( NM_001272122.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259051.1 Drosophila melanogaster nuclear factor I, transcript variant C (NfI), mRNA.

Price & Availability↑

CloneID	OFa00150
Clone ID Related Accession (Same CDS sequence)	NM_001272122.2
Accession Version	NM_001272122.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nuclear factor I, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jul 15, 2014 this sequence version replaced NM_001272122.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTATAC CAGAAACTTT AAGAGGCTGT ATGATCGAGA CGGACGTTTC TTCTTATTTG 
CAAACATCAT CATCGGGACA GGATGAATTT CACCCTTTTA TTGAGGCACT CTTACCGTAC 
GTCAAATCAT TTTCCTACTC TTGGTTTAAT TTGCAAGCCG CCAAACGAAA ATATTATAAG 
AAACATGAAA AACGTATGTC ACTGGAAGAA GAAAGACATT GTAAAGATGA ACTCCAGAAT 
GAAAAGACTG AGGTGAAGCA AAAGTGGGCT TCCCGTCTTC TTGGAAAACT CAGAAAAGAC 
ATTACACAAG AAAGTCGAGA GGATTTTGTG CAATCTATAA TCGGGAAAAG AAAATCAATT 
TGTGTTCTCT CAAACCCCGA TCAAAAGGGA AAAATGAGAC GAATTGACTG CTTAAGACAG 
GCTGACAAAG TATGGCGGCT TGATTTGGTC ATGGTTATAC TCTTCAAGGC GATTCCATTA 
GAAAGCACAG ATGGTGAGCG GTTAGAAAAA AATCCGGAAT GTTTACATCC AGGATTATGT 
GTAAATCCGT ACCACATTAA CGTGTCTGTT CGGGAATTGG ACTTGTACTT GGCCAACTTT 
ATAAATACTC ATAATTCAAT TAACAATACA AATACGACTT TCTCAACTAC TCCGGGCGTA 
AGATCGCCAC ATATAACTAT GTATAATCGA AGTAATGATC CAAACAGAAA AGATGAGGTC 
GCTGACATGA AAAATGATGT CGTTAAACAA AACCCATATA ATGGTGTTGT TTGTAATGAT 
ATTATTTTAG CAACTGGCGT GTTTTCTTCT CAAGAACTGT GGACATTATC TAAAGATTTA 
ATTTTAGATG AATCAAATGA TATAAACCTA AACTCTAGCC TTATAAAGAG GGAGAACGTT 
GGAGCTACCT ATGAGTGTAA TACATATCAA ATTAACTCTG AATCCTCAAT TTCTGCTGCT 
CAGAGTCTTG TTCAATCTGG GTCAATTGCG GCTAATCCGA TTCCACTTGG ATATAGCAAT 
CAGAGGATTA CACAATTATC ACAAAGTCCA TTGCCACGTA CAGAAGGCCC AAACGGCGAT 
TCCCATGATG CTAACAAAAT CGATCAAAGT AGCTCACCGC CAATCACGAG CACTGCATCA 
GATAATGGCA CAAGTAGTTT TTCTTTGATC GTGAGAGCTA CGGATAGCTC TATTGAAGAT 
CCAAAAACTT CACCCATTTC TTTGCATCGC TATACCTCTT TAAGTAGTCG GACGCTGGCC 
CAAGCTCTCC CACAACATAG TTGCTCCTTG CTACATTCGT CTTCCACTAG TCCGACTAAT 
ACCTTATATT ACCCCCAGCA TAATAATACA AGACCATCTC AAACAACTTC CGTTGAGGAA 
CAAAATCGTG TTGGCTCCGA CAAATATTCA ACGGAGCCTC AAGATATTTC GGACTTTGTC 
ACTTATGTTT GTCAAGACAC CAGTCATACG ACAACTATAA CAGGATCAGC TGAAAACCAT 
TCATTTCAAC ACTCCCATAC ACTCCCACAA GGTCATGGTC ATTCGCCGCA TTTTCAAATT 
CATCAACAGC TGCGATCTGC AAAATTAACG ACTTCACCAT CAACTCATTA TCACAGTACA 
ATGCTTCCGC CGATGCTGCC ACCAATGGCT CGACCTGTAG CTATAATTCG CAGTAGCAGT 
GATCTAACCT TAGTCCAATC CCCACCAACT TCTTTGCCAC TGACAGCCCA ATCTACAAGC 
TTGAACGACA ATAGTTGTAT AGCGAAACAT AGCCTGCCAA CGGATAATAG ACTTGCAAGT 
AACCTTGACA ACAGAAATAG CCCCAATAAC ACAGATGTCG TTTCACAACA CGAAATGACT 
GGAACTGCAT CTCCTCAACC GCAATCTCAC ACACCGACAT TAGCGAGTCC CCAATCTTAT 
CTGGATCCTG GGCGATGTAA GCTTTTATCA GCTGGCTCAA ACATAGGCAG AATTACAACA 
CAAATGTATC CCTCATCAAA TAATAGAGAA TATTTTAATC ACTTTCATTC GCAGCCCACA 
TCGCTTTTGG GATATGCCGG CTCTATTTCT ACAATGTCTG GAGTTATAAG TCCAACAGAC 
CTAACACTTT ACTCAGCTCC AATGGCTGTA TCACGTTCAA GTACCAGGAC TAGATGGAAC 
GAAGAGGAGC ATAATGTAAT TCCACAGTCT GCATCTAGTA CAAACATGGA TAACACCCAA 
GTTATATTAA TGGAAGACTC GACTGGTCGT TATATAGATG AGTATTCTAG TCGTGACTAT 
GTGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259051.1
CDS	139..2427
Translation	MFIPETLRGCMIETDVSSYLQTSSSGQDEFHPFIEALLPYVKSFSYSWFNLQAAKRKYYKKHEKRMSLEEERHCKDELQNEKTEVKQKWASRLLGKLRKDITQESREDFVQSIIGKRKSICVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKAIPLESTDGERLEKNPECLHPGLCVNPYHINVSVRELDLYLANFINTHNSINNTNTTFSTTPGVRSPHITMYNRSNDPNRKDEVADMKNDVVKQNPYNGVVCNDIILATGVFSSQELWTLSKDLILDESNDINLNSSLIKRENVGATYECNTYQINSESSISAAQSLVQSGSIAANPIPLGYSNQRITQLSQSPLPRTEGPNGDSHDANKIDQSSSPPITSTASDNGTSSFSLIVRATDSSIEDPKTSPISLHRYTSLSSRTLAQALPQHSCSLLHSSSTSPTNTLYYPQHNNTRPSQTTSVEEQNRVGSDKYSTEPQDISDFVTYVCQDTSHTTTITGSAENHSFQHSHTLPQGHGHSPHFQIHQQLRSAKLTTSPSTHYHSTMLPPMLPPMARPVAIIRSSSDLTLVQSPPTSLPLTAQSTSLNDNSCIAKHSLPTDNRLASNLDNRNSPNNTDVVSQHEMTGTASPQPQSHTPTLASPQSYLDPGRCKLLSAGSNIGRITTQMYPSSNNREYFNHFHSQPTSLLGYAGSISTMSGVISPTDLTLYSAPMAVSRSSTRTRWNEEEHNVIPQSASSTNMDNTQVILMEDSTGRYIDEYSSRDYVS

Target ORF information:

RefSeq Version	NM_001272122.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nuclear factor I, transcript variant C (NfI), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272122.2

ORF Insert Sequence:

ATGTTTATAC CAGAAACTTT AAGAGGCTGT ATGATCGAGA CGGACGTTTC TTCTTATTTG 
CAAACATCAT CATCGGGACA GGATGAATTT CACCCTTTTA TTGAGGCACT CTTACCGTAC 
GTCAAATCAT TTTCCTACTC TTGGTTTAAT TTGCAAGCCG CCAAACGAAA ATATTATAAG 
AAACATGAAA AACGTATGTC ACTGGAAGAA GAAAGACATT GTAAAGATGA ACTCCAGAAT 
GAAAAGACTG AGGTGAAGCA AAAGTGGGCT TCCCGTCTTC TTGGAAAACT CAGAAAAGAC 
ATTACACAAG AAAGTCGAGA GGATTTTGTG CAATCTATAA TCGGGAAAAG AAAATCAATT 
TGTGTTCTCT CAAACCCCGA TCAAAAGGGA AAAATGAGAC GAATTGACTG CTTAAGACAG 
GCTGACAAAG TATGGCGGCT TGATTTGGTC ATGGTTATAC TCTTCAAGGC GATTCCATTA 
GAAAGCACAG ATGGTGAGCG GTTAGAAAAA AATCCGGAAT GTTTACATCC AGGATTATGT 
GTAAATCCGT ACCACATTAA CGTGTCTGTT CGGGAATTGG ACTTGTACTT GGCCAACTTT 
ATAAATACTC ATAATTCAAT TAACAATACA AATACGACTT TCTCAACTAC TCCGGGCGTA 
AGATCGCCAC ATATAACTAT GTATAATCGA AGTAATGATC CAAACAGAAA AGATGAGGTC 
GCTGACATGA AAAATGATGT CGTTAAACAA AACCCATATA ATGGTGTTGT TTGTAATGAT 
ATTATTTTAG CAACTGGCGT GTTTTCTTCT CAAGAACTGT GGACATTATC TAAAGATTTA 
ATTTTAGATG AATCAAATGA TATAAACCTA AACTCTAGCC TTATAAAGAG GGAGAACGTT 
GGAGCTACCT ATGAGTGTAA TACATATCAA ATTAACTCTG AATCCTCAAT TTCTGCTGCT 
CAGAGTCTTG TTCAATCTGG GTCAATTGCG GCTAATCCGA TTCCACTTGG ATATAGCAAT 
CAGAGGATTA CACAATTATC ACAAAGTCCA TTGCCACGTA CAGAAGGCCC AAACGGCGAT 
TCCCATGATG CTAACAAAAT CGATCAAAGT AGCTCACCGC CAATCACGAG CACTGCATCA 
GATAATGGCA CAAGTAGTTT TTCTTTGATC GTGAGAGCTA CGGATAGCTC TATTGAAGAT 
CCAAAAACTT CACCCATTTC TTTGCATCGC TATACCTCTT TAAGTAGTCG GACGCTGGCC 
CAAGCTCTCC CACAACATAG TTGCTCCTTG CTACATTCGT CTTCCACTAG TCCGACTAAT 
ACCTTATATT ACCCCCAGCA TAATAATACA AGACCATCTC AAACAACTTC CGTTGAGGAA 
CAAAATCGTG TTGGCTCCGA CAAATATTCA ACGGAGCCTC AAGATATTTC GGACTTTGTC 
ACTTATGTTT GTCAAGACAC CAGTCATACG ACAACTATAA CAGGATCAGC TGAAAACCAT 
TCATTTCAAC ACTCCCATAC ACTCCCACAA GGTCATGGTC ATTCGCCGCA TTTTCAAATT 
CATCAACAGC TGCGATCTGC AAAATTAACG ACTTCACCAT CAACTCATTA TCACAGTACA 
ATGCTTCCGC CGATGCTGCC ACCAATGGCT CGACCTGTAG CTATAATTCG CAGTAGCAGT 
GATCTAACCT TAGTCCAATC CCCACCAACT TCTTTGCCAC TGACAGCCCA ATCTACAAGC 
TTGAACGACA ATAGTTGTAT AGCGAAACAT AGCCTGCCAA CGGATAATAG ACTTGCAAGT 
AACCTTGACA ACAGAAATAG CCCCAATAAC ACAGATGTCG TTTCACAACA CGAAATGACT 
GGAACTGCAT CTCCTCAACC GCAATCTCAC ACACCGACAT TAGCGAGTCC CCAATCTTAT 
CTGGATCCTG GGCGATGTAA GCTTTTATCA GCTGGCTCAA ACATAGGCAG AATTACAACA 
CAAATGTATC CCTCATCAAA TAATAGAGAA TATTTTAATC ACTTTCATTC GCAGCCCACA 
TCGCTTTTGG GATATGCCGG CTCTATTTCT ACAATGTCTG GAGTTATAAG TCCAACAGAC 
CTAACACTTT ACTCAGCTCC AATGGCTGTA TCACGTTCAA GTACCAGGAC TAGATGGAAC 
GAAGAGGAGC ATAATGTAAT TCCACAGTCT GCATCTAGTA CAAACATGGA TAACACCCAA 
GTTATATTAA TGGAAGACTC GACTGGTCGT TATATAGATG AGTATTCTAG TCGTGACTAT 
GTGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NfI ( NM_205870.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995592.2 Drosophila melanogaster nuclear factor I, transcript variant B (NfI), mRNA.

Price & Availability↑

CloneID	OFa00251
Clone ID Related Accession (Same CDS sequence)	NM_205870.3
Accession Version	NM_205870.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nuclear factor I, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_205870.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTATAC CAGAAACTTT AAGAGGCTGT ATGATCGAGA CGGACGTTTC TTCTTATTTG 
CAAACATCAT CATCGGGACA GGATGAATTT CACCCTTTTA TTGAGGCACT CTTACCGTAC 
GTCAAATCAT TTTCCTACTC TTGGTTTAAT TTGCAAGCCG CCAAACGAAA ATATTATAAG 
AAACATGAAA AACGTATGTC ACTGGAAGAA GAAAGACATT GTAAAGATGA ACTCCAGAAT 
GAAAAGACTG AGGTGAAGCA AAAGTGGGCT TCCCGTCTTC TTGGAAAACT CAGAAAAGAC 
ATTACACAAG AAAGTCGAGA GGATTTTGTG CAATCTATAA TCGGGAAAAG AAAATCAATT 
TGTGTTCTCT CAAACCCCGA TCAAAAGGGA AAAATGAGAC GAATTGACTG CTTAAGACAG 
GCTGACAAAG TATGGCGGCT TGATTTGGTC ATGGTTATAC TCTTCAAGGC GATTCCATTA 
GAAAGCACAG ATGGTGAGCG GTTAGAAAAA AATCCGGAAT GTTTACATCC AGGATTATGT 
GTAAATCCGT ACCACATTAA CGTGTCTGTT CGGGAATTGG ACTTGTACTT GGCCAACTTT 
ATAAATACTC ATAATTCAAT TAACAATACA AATACGACTT TCTCAACTAC TCCGGGCGTA 
AGATCGCCAC ATATAACTAT GTATAATCGA AGTAATGATC CAAACAGAAA AGATGAGGTC 
GCTGACATGA AAAATGATGT CGTTAAACAA AACCCATATA ATGGTGTTGT TTGTAATGAT 
ATTATTTTAG CAACTGGCGT GTTTTCTTCT CAAGAACTGT GGACATTATC TAAAGATTTA 
ATTTTAGATG AATCAAATGA TATAAACCTA AACTCTAGCC TTATAAAGAG GGAGAACGTT 
GGAGCTACCT ATGAGTGTAA TACATATCAA ATTAACTCTG AATCCTCAAT TTCTGCTGCT 
CAGAGTCTTG TTCAATCTGG GTCAATTGCG GCTAATCCGA TTCCACTTGG ATATAGCATT 
GATTTTATAG ATCAGAGGAT TACACAATTA TCACAAAGTC CATTGCCACG TACAGAAGGC 
CCAAACGGCG ATTCCCATGA TGCTAACAAA ATCGATCAAA GTAGCTCACC GCCAATCACG 
AGCACTGCAT CAGATAATGG CACAAGTAGT TTTTCTTTGA TCGTGAGAGC TACGGATAGC 
TCTATTGAAG ATCCAAAAAC TTCACCCATT TCTTTGCATC GCTATACCTC TTTAAGTAGT 
CGGACGCTGG CCCAAGCTCT CCCACAACAT AGTTGCTCCT TGCTACATTC GTCTTCCACT 
AGTCCGACTA ATACCTTATA TTACCCCCAG CATAATAATA CAAGACCATC TCAAACAACT 
TCCGTTGAGG AACAAAATCG TGTTGGCTCC GACAAATATT CAACGGAGCC TCAAGATATT 
TCGGACTTTG TCACTTATGT TTGTCAAGAC ACCAGTCATA CGACAACTAT AACAGGATCA 
GCTGAAAACC ATTCATTTCA ACACTCCCAT ACACTCCCAC AAGGTCATGG TCATTCGCCG 
CATTTTCAAA TTCATCAACA GCTGCGATCT GCAAAATTAA CGACTTCACC ATCAACTCAT 
TATCACAGTA CAATGCTTCC GCCGATGCTG CCACCAATGG CTCGACCTGT AGCTATAATT 
CGCAGTAGCA GTGATCTAAC CTTAGTCCAA TCCCCACCAA CTTCTTTGCC ACTGACAGCC 
CAATCTACAA GCTTGAACGA CAATAGTTGT ATAGCGAAAC ATAGCCTGCC AACGGATAAT 
AGACTTGCAA GTAACCTTGA CAACAGAAAT AGCCCCAATA ACACAGATGT CGTTTCACAA 
CACGAAATGA CTGGAACTGC ATCTCCTCAA CCGCAATCTC ACACACCGAC ATTAGCGAGT 
CCCCAATCTT ATCTGGATCC TGGGCGATGT AAGCTTTTAT CAGCTGGCTC AAACATAGGC 
AGAATTACAA CACAAATGTA TCCCTCATCA AATAATAGAG AATATTTTAA TCACTTTCAT 
TCGCAGCCCA CATCGCTTTT GGGATATGCC GGCTCTATTT CTACAATGTC TGGAGTTATA 
AGTCCAACAG ACCTAACACT TTACTCAGCT CCAATGGCTG TATCACGTTC AAGTACCAGG 
ACTAGATGGA ACGAAGAGGA GCATAATGTA ATTCCACAGT CTGCATCTAG TACAAACATG 
GATAACACCC AAGTTATATT AATGGAAGAC TCGACTGGTC GTTATATAGA TGAGTATTCT 
AGTCGTGACT ATGTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995592.2
CDS	139..2439
Translation	MFIPETLRGCMIETDVSSYLQTSSSGQDEFHPFIEALLPYVKSFSYSWFNLQAAKRKYYKKHEKRMSLEEERHCKDELQNEKTEVKQKWASRLLGKLRKDITQESREDFVQSIIGKRKSICVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKAIPLESTDGERLEKNPECLHPGLCVNPYHINVSVRELDLYLANFINTHNSINNTNTTFSTTPGVRSPHITMYNRSNDPNRKDEVADMKNDVVKQNPYNGVVCNDIILATGVFSSQELWTLSKDLILDESNDINLNSSLIKRENVGATYECNTYQINSESSISAAQSLVQSGSIAANPIPLGYSIDFIDQRITQLSQSPLPRTEGPNGDSHDANKIDQSSSPPITSTASDNGTSSFSLIVRATDSSIEDPKTSPISLHRYTSLSSRTLAQALPQHSCSLLHSSSTSPTNTLYYPQHNNTRPSQTTSVEEQNRVGSDKYSTEPQDISDFVTYVCQDTSHTTTITGSAENHSFQHSHTLPQGHGHSPHFQIHQQLRSAKLTTSPSTHYHSTMLPPMLPPMARPVAIIRSSSDLTLVQSPPTSLPLTAQSTSLNDNSCIAKHSLPTDNRLASNLDNRNSPNNTDVVSQHEMTGTASPQPQSHTPTLASPQSYLDPGRCKLLSAGSNIGRITTQMYPSSNNREYFNHFHSQPTSLLGYAGSISTMSGVISPTDLTLYSAPMAVSRSSTRTRWNEEEHNVIPQSASSTNMDNTQVILMEDSTGRYIDEYSSRDYVS

Target ORF information:

RefSeq Version	NM_205870.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nuclear factor I, transcript variant B (NfI), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205870.3

ORF Insert Sequence:

ATGTTTATAC CAGAAACTTT AAGAGGCTGT ATGATCGAGA CGGACGTTTC TTCTTATTTG 
CAAACATCAT CATCGGGACA GGATGAATTT CACCCTTTTA TTGAGGCACT CTTACCGTAC 
GTCAAATCAT TTTCCTACTC TTGGTTTAAT TTGCAAGCCG CCAAACGAAA ATATTATAAG 
AAACATGAAA AACGTATGTC ACTGGAAGAA GAAAGACATT GTAAAGATGA ACTCCAGAAT 
GAAAAGACTG AGGTGAAGCA AAAGTGGGCT TCCCGTCTTC TTGGAAAACT CAGAAAAGAC 
ATTACACAAG AAAGTCGAGA GGATTTTGTG CAATCTATAA TCGGGAAAAG AAAATCAATT 
TGTGTTCTCT CAAACCCCGA TCAAAAGGGA AAAATGAGAC GAATTGACTG CTTAAGACAG 
GCTGACAAAG TATGGCGGCT TGATTTGGTC ATGGTTATAC TCTTCAAGGC GATTCCATTA 
GAAAGCACAG ATGGTGAGCG GTTAGAAAAA AATCCGGAAT GTTTACATCC AGGATTATGT 
GTAAATCCGT ACCACATTAA CGTGTCTGTT CGGGAATTGG ACTTGTACTT GGCCAACTTT 
ATAAATACTC ATAATTCAAT TAACAATACA AATACGACTT TCTCAACTAC TCCGGGCGTA 
AGATCGCCAC ATATAACTAT GTATAATCGA AGTAATGATC CAAACAGAAA AGATGAGGTC 
GCTGACATGA AAAATGATGT CGTTAAACAA AACCCATATA ATGGTGTTGT TTGTAATGAT 
ATTATTTTAG CAACTGGCGT GTTTTCTTCT CAAGAACTGT GGACATTATC TAAAGATTTA 
ATTTTAGATG AATCAAATGA TATAAACCTA AACTCTAGCC TTATAAAGAG GGAGAACGTT 
GGAGCTACCT ATGAGTGTAA TACATATCAA ATTAACTCTG AATCCTCAAT TTCTGCTGCT 
CAGAGTCTTG TTCAATCTGG GTCAATTGCG GCTAATCCGA TTCCACTTGG ATATAGCATT 
GATTTTATAG ATCAGAGGAT TACACAATTA TCACAAAGTC CATTGCCACG TACAGAAGGC 
CCAAACGGCG ATTCCCATGA TGCTAACAAA ATCGATCAAA GTAGCTCACC GCCAATCACG 
AGCACTGCAT CAGATAATGG CACAAGTAGT TTTTCTTTGA TCGTGAGAGC TACGGATAGC 
TCTATTGAAG ATCCAAAAAC TTCACCCATT TCTTTGCATC GCTATACCTC TTTAAGTAGT 
CGGACGCTGG CCCAAGCTCT CCCACAACAT AGTTGCTCCT TGCTACATTC GTCTTCCACT 
AGTCCGACTA ATACCTTATA TTACCCCCAG CATAATAATA CAAGACCATC TCAAACAACT 
TCCGTTGAGG AACAAAATCG TGTTGGCTCC GACAAATATT CAACGGAGCC TCAAGATATT 
TCGGACTTTG TCACTTATGT TTGTCAAGAC ACCAGTCATA CGACAACTAT AACAGGATCA 
GCTGAAAACC ATTCATTTCA ACACTCCCAT ACACTCCCAC AAGGTCATGG TCATTCGCCG 
CATTTTCAAA TTCATCAACA GCTGCGATCT GCAAAATTAA CGACTTCACC ATCAACTCAT 
TATCACAGTA CAATGCTTCC GCCGATGCTG CCACCAATGG CTCGACCTGT AGCTATAATT 
CGCAGTAGCA GTGATCTAAC CTTAGTCCAA TCCCCACCAA CTTCTTTGCC ACTGACAGCC 
CAATCTACAA GCTTGAACGA CAATAGTTGT ATAGCGAAAC ATAGCCTGCC AACGGATAAT 
AGACTTGCAA GTAACCTTGA CAACAGAAAT AGCCCCAATA ACACAGATGT CGTTTCACAA 
CACGAAATGA CTGGAACTGC ATCTCCTCAA CCGCAATCTC ACACACCGAC ATTAGCGAGT 
CCCCAATCTT ATCTGGATCC TGGGCGATGT AAGCTTTTAT CAGCTGGCTC AAACATAGGC 
AGAATTACAA CACAAATGTA TCCCTCATCA AATAATAGAG AATATTTTAA TCACTTTCAT 
TCGCAGCCCA CATCGCTTTT GGGATATGCC GGCTCTATTT CTACAATGTC TGGAGTTATA 
AGTCCAACAG ACCTAACACT TTACTCAGCT CCAATGGCTG TATCACGTTC AAGTACCAGG 
ACTAGATGGA ACGAAGAGGA GCATAATGTA ATTCCACAGT CTGCATCTAG TACAAACATG 
GATAACACCC AAGTTATATT AATGGAAGAC TCGACTGGTC GTTATATAGA TGAGTATTCT 
AGTCGTGACT ATGTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
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The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
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Combined evidence annotation of transposable elements in genome sequences.
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