sad cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	sad
Entrez Gene ID	44858
Full Name	shadow
Synonyms	315a1,CG14728,CYP315A1,Cyp315a1,Dmel\CG14728,Sad
General protein information	Preferred Names shadow Names CG14728 gene product from transcript CG14728-RA CG14728-PA cytochrome P450 ecdysteroid hydroxylase karmoisin ghost sad-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 87A1-87A1\|3-51 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_141866.2	NP_650123.1	shadow

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	sad	NP_650123.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000284	XP_310837.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

codes for a mitochondrial cytochrome P450 that mediates the last two hydroxylation reactions in the ecdysteroidogenic pathway in Drosophila
Title: Molecular and biochemical characterization of two P450 enzymes in the ecdysteroidogenic pathway of Drosophila melanogaster.

The following sad gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sad cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06867	NM_141866.2 Latest version!	Drosophila melanogaster shadow (sad), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sad ( NM_141866.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650123.1 Drosophila melanogaster shadow (sad), mRNA.

Price & Availability↑

CloneID	OFa06867
Clone ID Related Accession (Same CDS sequence)	NM_141866.2
Accession Version	NM_141866.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	shadow
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Mar 18, 2004 this sequence version replaced NM_141866.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCGAGA AGAGGGAGCG GCCGGGCCCG CTGCGCTGGC TGAGACACCT GCTCGACCAG 
CTCCTGGTGC GAATCCTTAG CCTGAGCCTC TTCCGTTCGC GCTGCGATCC GCCGCCTTTG 
CAGCGTTTTC CCGCAACGGA ACTACCGCCT GCCGTCGCCG CCAAATACGT GCCCATTCCA 
AGGGTGAAGG GACTTCCGGT AGTTGGGACA CTTGTGGATC TTATAGCCGC CGGCGGAGCC 
ACGCACCTTC ATAAGTACAT CGATGCGAGG CACAAGCAGT ATGGTCCCAT TTTCCGGGAG 
CGATTGGGCG GTACCCAGGA TGCAGTGTTC GTATCGTCCG CAAATCTCAT GCGCGGAGTC 
TTCCAGCACG AGGGTCAGTA TCCGCAGCAT CCGCTGCCGG ATGCCTGGAC GCTGTATAAC 
CAGCAACATG CCTGCCAACG GGGACTGTTC TTCATGGAGG GCGCCGAGTG GCTGCACAAC 
CGACGCATAC TTAATCGACT GCTGCTCAAC GGAAATTTGA ATTGGATGGA CGTGCATATT 
GAGAGCTGTA CCAGACGAAT GGTGGATCAG TGGAAAAGAC GCACTGCGGA GGCGGCGGCG 
ATTCCGCTAG CGGAGAGTGG TGAAATACGA AGCTACGAAC TGCCCCTGTT GGAACAACAG 
CTCTACCGTT GGTCCATAGA AGTTCTGTGC TGCATCATGT TTGGCACCAG CGTGCTCACC 
TGCCCCAAGA TCCAGTCCTC GCTGGACTAC TTCACGCAGA TTGTGCACAA GGTGTTTGAG 
CATAGCTCGC GACTGATGAC ATTCCCGCCT CGCTTGGCCC AGATTTTGCG CCTGCCCATC 
TGGCGGGATT TCGAGGCCAA TGTGGATGAG GTGCTGCGTG AGGGAGCTGC CATAATCGAT 
CACTGCATCA GAGTGCAGGA GGACCAAAGG AGACCGCACG ATGAGGCGCT TTACCATCGC 
CTCCAGGCGG CGGATGTGCC AGGCGATATG ATCAAGCGGA TATTTGTAGA CTTGGTCATT 
GCAGCAGGTG ACACGACCGC ATTCAGCAGT CAGTGGGCTT TGTTTGCCCT TTCAAAGGAG 
CCGAGGCTCC AGCAACGACT GGCCAAGGAG CGAGCTACCA ATGATTCTCG CCTGATGCAC 
GGCCTGATCA AGGAGTCCCT GCGTCTGTAC CCCGTAGCTC CCTTCATTGG CCGATATCTG 
CCGCAGGACG CGCAACTTGG CGGTCACTTT ATCGAAAAGG ATACCATGGT GCTGCTCTCC 
TTGTACACGG CAGGTCGCGA TCCATCACAC TTTGAGCAGC CGGAACGTGT GCTCCCGGAG 
CGCTGGTGCA TTGGAGAGAC GGAGCAGGTG CATAAGTCAC ACGGCAGTCT GCCTTTTGCC 
ATCGGCCAGC GGTCTTGCAT TGGTCGCCGT GTGGCACTCA AGCAGCTCCA CTCCCTGCTG 
GGCCGATGTG CTGCTCAGTT TGAGATGAGC TGCCTTAACG AGATGCCCGT TGACAGCGTA 
CTCCGCATGG TCACCGTGCC CGATCGGACT TTGCGTTTAG CCCTTCGGCC GCGAACCGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650123.1
CDS	107..1669
Translation	MTEKRERPGPLRWLRHLLDQLLVRILSLSLFRSRCDPPPLQRFPATELPPAVAAKYVPIPRVKGLPVVGTLVDLIAAGGATHLHKYIDARHKQYGPIFRERLGGTQDAVFVSSANLMRGVFQHEGQYPQHPLPDAWTLYNQQHACQRGLFFMEGAEWLHNRRILNRLLLNGNLNWMDVHIESCTRRMVDQWKRRTAEAAAIPLAESGEIRSYELPLLEQQLYRWSIEVLCCIMFGTSVLTCPKIQSSLDYFTQIVHKVFEHSSRLMTFPPRLAQILRLPIWRDFEANVDEVLREGAAIIDHCIRVQEDQRRPHDEALYHRLQAADVPGDMIKRIFVDLVIAAGDTTAFSSQWALFALSKEPRLQQRLAKERATNDSRLMHGLIKESLRLYPVAPFIGRYLPQDAQLGGHFIEKDTMVLLSLYTAGRDPSHFEQPERVLPERWCIGETEQVHKSHGSLPFAIGQRSCIGRRVALKQLHSLLGRCAAQFEMSCLNEMPVDSVLRMVTVPDRTLRLALRPRTE

Target ORF information:

RefSeq Version	NM_141866.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster shadow (sad), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141866.2

ORF Insert Sequence:

ATGACCGAGA AGAGGGAGCG GCCGGGCCCG CTGCGCTGGC TGAGACACCT GCTCGACCAG 
CTCCTGGTGC GAATCCTTAG CCTGAGCCTC TTCCGTTCGC GCTGCGATCC GCCGCCTTTG 
CAGCGTTTTC CCGCAACGGA ACTACCGCCT GCCGTCGCCG CCAAATACGT GCCCATTCCA 
AGGGTGAAGG GACTTCCGGT AGTTGGGACA CTTGTGGATC TTATAGCCGC CGGCGGAGCC 
ACGCACCTTC ATAAGTACAT CGATGCGAGG CACAAGCAGT ATGGTCCCAT TTTCCGGGAG 
CGATTGGGCG GTACCCAGGA TGCAGTGTTC GTATCGTCCG CAAATCTCAT GCGCGGAGTC 
TTCCAGCACG AGGGTCAGTA TCCGCAGCAT CCGCTGCCGG ATGCCTGGAC GCTGTATAAC 
CAGCAACATG CCTGCCAACG GGGACTGTTC TTCATGGAGG GCGCCGAGTG GCTGCACAAC 
CGACGCATAC TTAATCGACT GCTGCTCAAC GGAAATTTGA ATTGGATGGA CGTGCATATT 
GAGAGCTGTA CCAGACGAAT GGTGGATCAG TGGAAAAGAC GCACTGCGGA GGCGGCGGCG 
ATTCCGCTAG CGGAGAGTGG TGAAATACGA AGCTACGAAC TGCCCCTGTT GGAACAACAG 
CTCTACCGTT GGTCCATAGA AGTTCTGTGC TGCATCATGT TTGGCACCAG CGTGCTCACC 
TGCCCCAAGA TCCAGTCCTC GCTGGACTAC TTCACGCAGA TTGTGCACAA GGTGTTTGAG 
CATAGCTCGC GACTGATGAC ATTCCCGCCT CGCTTGGCCC AGATTTTGCG CCTGCCCATC 
TGGCGGGATT TCGAGGCCAA TGTGGATGAG GTGCTGCGTG AGGGAGCTGC CATAATCGAT 
CACTGCATCA GAGTGCAGGA GGACCAAAGG AGACCGCACG ATGAGGCGCT TTACCATCGC 
CTCCAGGCGG CGGATGTGCC AGGCGATATG ATCAAGCGGA TATTTGTAGA CTTGGTCATT 
GCAGCAGGTG ACACGACCGC ATTCAGCAGT CAGTGGGCTT TGTTTGCCCT TTCAAAGGAG 
CCGAGGCTCC AGCAACGACT GGCCAAGGAG CGAGCTACCA ATGATTCTCG CCTGATGCAC 
GGCCTGATCA AGGAGTCCCT GCGTCTGTAC CCCGTAGCTC CCTTCATTGG CCGATATCTG 
CCGCAGGACG CGCAACTTGG CGGTCACTTT ATCGAAAAGG ATACCATGGT GCTGCTCTCC 
TTGTACACGG CAGGTCGCGA TCCATCACAC TTTGAGCAGC CGGAACGTGT GCTCCCGGAG 
CGCTGGTGCA TTGGAGAGAC GGAGCAGGTG CATAAGTCAC ACGGCAGTCT GCCTTTTGCC 
ATCGGCCAGC GGTCTTGCAT TGGTCGCCGT GTGGCACTCA AGCAGCTCCA CTCCCTGCTG 
GGCCGATGTG CTGCTCAGTT TGAGATGAGC TGCCTTAACG AGATGCCCGT TGACAGCGTA 
CTCCGCATGG TCACCGTGCC CGATCGGACT TTGCGTTTAG CCCTTCGGCC GCGAACCGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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sad cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

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Target ORF information:

ORF Insert Sequence:

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