Ttc7 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ttc7
Entrez Gene ID	48387
Full Name	lethal (2) k14710
Synonyms	BcDNA:LD21719,CG8325,Dmel\CG8325,l(2)k15603
General protein information	Preferred Names lethal (2) k14710 Names CG8325 gene product from transcript CG8325-RB CG8325-PA CG8325-PB l(2)k14710-PA l(2)k14710-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 42A13-42A13

mRNA and Protein(s)

mRNA	Protein	Name
NM_143806.4	NP_652063.1	tetratricopeptide repeat domain 7, isoform A
NM_001259218.2	NP_001246147.1	tetratricopeptide repeat domain 7, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TTC7B	XP_510118.2
Bos taurus (cattle)	TTC7B	XP_002691137.2
Homo sapiens (human)	TTC7B	NP_001010854.1
	TTC7B	XP_001090821.1
Mus musculus (house mouse)	Ttc7b	NP_001028385.1
Caenorhabditis elegans (roundworm)	Y39A3CR.3	NP_001254845.1
Xenopus tropicalis (tropical clawed frog)	ttc7b	NP_001090808.1
Canis lupus familiaris (dog)	TTC7B	XP_005623799.1
Gallus gallus (chicken)	TTC7B	NP_001006484.1
Danio rerio (zebrafish)	ttc7b	NP_001074072.1
Drosophila melanogaster (fruit fly)	l(2)k14710	NP_652063.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010587	XP_314552.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ttc7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ttc7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11458	NM_143806.4 Latest version!	Drosophila melanogaster tetratricopeptide repeat domain 7, transcript variant A (Ttc7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa12848	NM_001259218.2 Latest version!	Drosophila melanogaster tetratricopeptide repeat domain 7, transcript variant B (Ttc7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ttc7 ( NM_143806.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_652063.1 Drosophila melanogaster tetratricopeptide repeat domain 7, transcript variant A (Ttc7), mRNA.

Price & Availability↑

CloneID	OFa11458
Clone ID Related Accession (Same CDS sequence)	NM_143806.4
Accession Version	NM_143806.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tetratricopeptide repeat domain 7, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_143806.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAACA AGAACATGCG TAACACCACA ACCAAAGTGG AGGCCCTGAT TGAGAGCTGT 
CGCAGCGAGG GCAAGTGGCA CCGGGTCATC GAGCTAACGG ATGAACTGAA GACCGGGTCC 
CCGCACAATG AGTGCCTGGC CAACTTTCTG GTGGGGGAAG CTCGTCTGGA GAGTTACCTG 
GAGGAAAACG CTCTCGCGTC AGACTCAAAT TTTGGTCGCG CCAAGTCTGG ATTGGCGGAG 
GCCCGGCGTT TCCTTCACTT GGCTTTGGGT GAGAGTGGCC AGAAGGCGGG CATCGCCCTG 
GACGCCTATT TGCTGCTGGC CAAGCTGTGC TTTGCCTGCG GCGAGTATGA GCAGAGTCTA 
GACAATTTCG TCAAGGCAGA ACTCAACACG CTTGCCGAGA AGGAGCTGAC CCTTCGCAGC 
CTGAAGATCC TTGCGGAATC GTATGCCATC AAGGGATTGT GTCTGGAGCA GCAGACTACG 
AAGCCGTCAT CTAAGTTTAA GAAGGCCGAA AAGGACACGG AAATGATTAG CTGTTTTGAG 
CGCGCATCTG ATCTGGGGCT GCTCTATCTG CAGGAATACG ATCTCGTTAG TGGAAGCAGT 
GGCTCGTCCA ACAACTCGAC AGCTGGTTCT ACGTTGAATG TAAACGCCTC TACTGTGCAG 
CCGTCGAGCA GCAGTTTTGC AATCAGCAGT ACAATACCGG CGAGTGGTCC AAGTGGACTG 
GAAATGAACC GCAGGATGGG CGCCATCCTA GAGACCGCCC TGCAACGGGC ACCCATAGTG 
CTTATTAAGA CGGAAAAGCT TCAAGAGGCC GTTGAACGGT ATCGAATCAT GCTAAACGCC 
ATCGAAACGA GGGCTACTCA ATCGTTGCGC CTCACGCTGG CCCGCCAGCT AGCTGAGGTT 
CTTTTGAGAG GGGTCTCGGG CACGATTTAC TCGCCTCCTT TTACCGGAAA ATCTGGAGGT 
GGGACGCTGC GAGGAGGATC CTCCAAGAAA CTTTGGAAAC CGCGTAAATA CGCAGCCCGC 
CAGCAGTTTA ACCCTCGGAA CCAGCAGGAA GAGGTAATTC TGTTGCTACT CATAGCCGAG 
GCACTGGCTG TGAGGGATAC AGTTTTGTCG CAGAGTCCAG AGTTTAGACA GGCCCGTCAG 
CATGCTATGG GCAACGTTAC GGCCGTCTAT GACCTCCTGA CGTTGGCTAC TGTGCGCTGG 
GGGCTCGTCC AGCTATTAAA TGAGTCCTTT GAGAAGGCGC TAAAGTTTAG CTTTGGTGAA 
CAGCATGTGT GGCGGCAGTA CGGCTTAAGT TTGATGGCAG CCGAAAAGCA CTCGCACGCT 
TTAAGGGTCT TGCAAGAATC AATGAAGTTG ACTCCTAGTG ATCCTTTGCC ATGTCTGTTG 
GCTTCTCGCC TTTGCTACGA GAGTCTGGAG ACGGTAAAGC AAGGTCTGGA CTATGCTCAG 
CAAGCGCTGA AGCGCGAAGT AAAGGGCTTG CGACCATCGC GAAGCCAACT CTTTGTGGGC 
ATCGGTCACC AACAGCTAGC CATCCAGTCA AATCTTAAAA GCGAGCGAGA TGCTTGTCAC 
AAGCTGGCTT TGGACGCCCT GGAGCGCGCT GTGCAGTTTG ATGGGAACGA CCACCTGGCG 
GAATACTACT TGTCGTTGCA GTACGCACTT CTGGGACAGC TGGCGGAGGC ATTGGTTCAT 
ATCCGTTTCG CGCTGGCGTT GCGTATGGAA CATGCGCCAT GTCTACACCT GTTCGCACTG 
TTGCTGACAT CGTCGCGCCG ACCTCGTGAA GCTTTGGGAG TTGTTGAGGA TGCTTTACAC 
GAGTTTCCCG ATAACCTGCA GCTACTGCAC GTTAAGGCAC ATCTTCAGCT GCATCTAGAG 
GACGCGGAGA CGGCGTTGGG CACTGTGCAG CACATGCTGG CCGTGTGGCG GGACGTTTAC 
GAGGCCCAGC TAGCGGGAGA GGAGGAAAAG CACTCAGACA CCAAGAGTGG TGTTCACTTG 
GCACATTCCT CACAGATGTC CGACAAGGAT TCAAATTCTG TGTACGCGGC TTCATTGGCT 
GCAGTCTCCC GCGTTGAACA GGCTCTGAGT GAAGCAGCAA GCTCATTGAG CTCATTTACG 
CAGCGTCCTG GACCCCGACG ACCCTGGATG CTACAGATTG AAATATGGCT TCTGCTGGCT 
GATGTCTATC TGCGGATTGA TCAGCCGAAC GAGGCACTCA ACTGCATACA CGAAGCCTCA 
CAGATTTATC CGCTTTCGCA TCAGATTATG TTTATGCGTG GCCAGGTGCA TGTCTATTTG 
GAGCAATGGT TTGACGCCAA GCAATGTTTC CTGAACGCCG TGGCCGCCAA CCCAAATCAC 
ACAGAGGCTT TGCGTGCGCT TGGAGAGGCG CATTTGGTAC TGGGCGAGCC GAGGTTGGCT 
GAAAAAATGC TAAAAGATGC GGCCAAACTG GATCCGAGCT GTCCAAAAAT TTGGTTCGCA 
CTGGGAAAGG TGATGGAGAT CCTGGGCGAT TTCCATGCCT CAGCCGATTG CTTCGCCACG 
TCGCTGCAGT TAGAGCCATC ATGTCCGGTG CTACCTTTTA CTTCTATACC TTTGGTGTTT 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_652063.1
CDS	182..2767
Translation	MANKNMRNTTTKVEALIESCRSEGKWHRVIELTDELKTGSPHNECLANFLVGEARLESYLEENALASDSNFGRAKSGLAEARRFLHLALGESGQKAGIALDAYLLLAKLCFACGEYEQSLDNFVKAELNTLAEKELTLRSLKILAESYAIKGLCLEQQTTKPSSKFKKAEKDTEMISCFERASDLGLLYLQEYDLVSGSSGSSNNSTAGSTLNVNASTVQPSSSSFAISSTIPASGPSGLEMNRRMGAILETALQRAPIVLIKTEKLQEAVERYRIMLNAIETRATQSLRLTLARQLAEVLLRGVSGTIYSPPFTGKSGGGTLRGGSSKKLWKPRKYAARQQFNPRNQQEEVILLLLIAEALAVRDTVLSQSPEFRQARQHAMGNVTAVYDLLTLATVRWGLVQLLNESFEKALKFSFGEQHVWRQYGLSLMAAEKHSHALRVLQESMKLTPSDPLPCLLASRLCYESLETVKQGLDYAQQALKREVKGLRPSRSQLFVGIGHQQLAIQSNLKSERDACHKLALDALERAVQFDGNDHLAEYYLSLQYALLGQLAEALVHIRFALALRMEHAPCLHLFALLLTSSRRPREALGVVEDALHEFPDNLQLLHVKAHLQLHLEDAETALGTVQHMLAVWRDVYEAQLAGEEEKHSDTKSGVHLAHSSQMSDKDSNSVYAASLAAVSRVEQALSEAASSLSSFTQRPGPRRPWMLQIEIWLLLADVYLRIDQPNEALNCIHEASQIYPLSHQIMFMRGQVHVYLEQWFDAKQCFLNAVAANPNHTEALRALGEAHLVLGEPRLAEKMLKDAAKLDPSCPKIWFALGKVMEILGDFHASADCFATSLQLEPSCPVLPFTSIPLVFE

Target ORF information:

RefSeq Version	NM_143806.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tetratricopeptide repeat domain 7, transcript variant A (Ttc7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143806.4

ORF Insert Sequence:

ATGGCCAACA AGAACATGCG TAACACCACA ACCAAAGTGG AGGCCCTGAT TGAGAGCTGT 
CGCAGCGAGG GCAAGTGGCA CCGGGTCATC GAGCTAACGG ATGAACTGAA GACCGGGTCC 
CCGCACAATG AGTGCCTGGC CAACTTTCTG GTGGGGGAAG CTCGTCTGGA GAGTTACCTG 
GAGGAAAACG CTCTCGCGTC AGACTCAAAT TTTGGTCGCG CCAAGTCTGG ATTGGCGGAG 
GCCCGGCGTT TCCTTCACTT GGCTTTGGGT GAGAGTGGCC AGAAGGCGGG CATCGCCCTG 
GACGCCTATT TGCTGCTGGC CAAGCTGTGC TTTGCCTGCG GCGAGTATGA GCAGAGTCTA 
GACAATTTCG TCAAGGCAGA ACTCAACACG CTTGCCGAGA AGGAGCTGAC CCTTCGCAGC 
CTGAAGATCC TTGCGGAATC GTATGCCATC AAGGGATTGT GTCTGGAGCA GCAGACTACG 
AAGCCGTCAT CTAAGTTTAA GAAGGCCGAA AAGGACACGG AAATGATTAG CTGTTTTGAG 
CGCGCATCTG ATCTGGGGCT GCTCTATCTG CAGGAATACG ATCTCGTTAG TGGAAGCAGT 
GGCTCGTCCA ACAACTCGAC AGCTGGTTCT ACGTTGAATG TAAACGCCTC TACTGTGCAG 
CCGTCGAGCA GCAGTTTTGC AATCAGCAGT ACAATACCGG CGAGTGGTCC AAGTGGACTG 
GAAATGAACC GCAGGATGGG CGCCATCCTA GAGACCGCCC TGCAACGGGC ACCCATAGTG 
CTTATTAAGA CGGAAAAGCT TCAAGAGGCC GTTGAACGGT ATCGAATCAT GCTAAACGCC 
ATCGAAACGA GGGCTACTCA ATCGTTGCGC CTCACGCTGG CCCGCCAGCT AGCTGAGGTT 
CTTTTGAGAG GGGTCTCGGG CACGATTTAC TCGCCTCCTT TTACCGGAAA ATCTGGAGGT 
GGGACGCTGC GAGGAGGATC CTCCAAGAAA CTTTGGAAAC CGCGTAAATA CGCAGCCCGC 
CAGCAGTTTA ACCCTCGGAA CCAGCAGGAA GAGGTAATTC TGTTGCTACT CATAGCCGAG 
GCACTGGCTG TGAGGGATAC AGTTTTGTCG CAGAGTCCAG AGTTTAGACA GGCCCGTCAG 
CATGCTATGG GCAACGTTAC GGCCGTCTAT GACCTCCTGA CGTTGGCTAC TGTGCGCTGG 
GGGCTCGTCC AGCTATTAAA TGAGTCCTTT GAGAAGGCGC TAAAGTTTAG CTTTGGTGAA 
CAGCATGTGT GGCGGCAGTA CGGCTTAAGT TTGATGGCAG CCGAAAAGCA CTCGCACGCT 
TTAAGGGTCT TGCAAGAATC AATGAAGTTG ACTCCTAGTG ATCCTTTGCC ATGTCTGTTG 
GCTTCTCGCC TTTGCTACGA GAGTCTGGAG ACGGTAAAGC AAGGTCTGGA CTATGCTCAG 
CAAGCGCTGA AGCGCGAAGT AAAGGGCTTG CGACCATCGC GAAGCCAACT CTTTGTGGGC 
ATCGGTCACC AACAGCTAGC CATCCAGTCA AATCTTAAAA GCGAGCGAGA TGCTTGTCAC 
AAGCTGGCTT TGGACGCCCT GGAGCGCGCT GTGCAGTTTG ATGGGAACGA CCACCTGGCG 
GAATACTACT TGTCGTTGCA GTACGCACTT CTGGGACAGC TGGCGGAGGC ATTGGTTCAT 
ATCCGTTTCG CGCTGGCGTT GCGTATGGAA CATGCGCCAT GTCTACACCT GTTCGCACTG 
TTGCTGACAT CGTCGCGCCG ACCTCGTGAA GCTTTGGGAG TTGTTGAGGA TGCTTTACAC 
GAGTTTCCCG ATAACCTGCA GCTACTGCAC GTTAAGGCAC ATCTTCAGCT GCATCTAGAG 
GACGCGGAGA CGGCGTTGGG CACTGTGCAG CACATGCTGG CCGTGTGGCG GGACGTTTAC 
GAGGCCCAGC TAGCGGGAGA GGAGGAAAAG CACTCAGACA CCAAGAGTGG TGTTCACTTG 
GCACATTCCT CACAGATGTC CGACAAGGAT TCAAATTCTG TGTACGCGGC TTCATTGGCT 
GCAGTCTCCC GCGTTGAACA GGCTCTGAGT GAAGCAGCAA GCTCATTGAG CTCATTTACG 
CAGCGTCCTG GACCCCGACG ACCCTGGATG CTACAGATTG AAATATGGCT TCTGCTGGCT 
GATGTCTATC TGCGGATTGA TCAGCCGAAC GAGGCACTCA ACTGCATACA CGAAGCCTCA 
CAGATTTATC CGCTTTCGCA TCAGATTATG TTTATGCGTG GCCAGGTGCA TGTCTATTTG 
GAGCAATGGT TTGACGCCAA GCAATGTTTC CTGAACGCCG TGGCCGCCAA CCCAAATCAC 
ACAGAGGCTT TGCGTGCGCT TGGAGAGGCG CATTTGGTAC TGGGCGAGCC GAGGTTGGCT 
GAAAAAATGC TAAAAGATGC GGCCAAACTG GATCCGAGCT GTCCAAAAAT TTGGTTCGCA 
CTGGGAAAGG TGATGGAGAT CCTGGGCGAT TTCCATGCCT CAGCCGATTG CTTCGCCACG 
TCGCTGCAGT TAGAGCCATC ATGTCCGGTG CTACCTTTTA CTTCTATACC TTTGGTGTTT 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ttc7 ( NM_001259218.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246147.1 Drosophila melanogaster tetratricopeptide repeat domain 7, transcript variant B (Ttc7), mRNA.

Price & Availability↑

CloneID	OFa12848
Clone ID Related Accession (Same CDS sequence)	NM_001259218.2
Accession Version	NM_001259218.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tetratricopeptide repeat domain 7, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001259218.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAACA AGAACATGCG TAACACCACA ACCAAAGTGG AGGCCCTGAT TGAGAGCTGT 
CGCAGCGAGG GCAAGTGGCA CCGGGTCATC GAGCTAACGG ATGAACTGAA GACCGGGTCC 
CCGCACAATG AGTGCCTGGC CAACTTTCTG GTGGGGGAAG CTCGTCTGGA GAGTTACCTG 
GAGGAAAACG CTCTCGCGTC AGACTCAAAT TTTGGTCGCG CCAAGTCTGG ATTGGCGGAG 
GCCCGGCGTT TCCTTCACTT GGCTTTGGGT GAGAGTGGCC AGAAGGCGGG CATCGCCCTG 
GACGCCTATT TGCTGCTGGC CAAGCTGTGC TTTGCCTGCG GCGAGTATGA GCAGAGTCTA 
GACAATTTCG TCAAGGCAGA ACTCAACACG CTTGCCGAGA AGGAGCTGAC CCTTCGCAGC 
CTGAAGATCC TTGCGGAATC GTATGCCATC AAGGGATTGT GTCTGGAGCA GCAGACTACG 
AAGCCGTCAT CTAAGTTTAA GAAGGCCGAA AAGGACACGG AAATGATTAG CTGTTTTGAG 
CGCGCATCTG ATCTGGGGCT GCTCTATCTG CAGGAATACG ATCTCGTTAG TGGAAGCAGT 
GGCTCGTCCA ACAACTCGAC AGCTGGTTCT ACGTTGAATG TAAACGCCTC TACTGTGCAG 
CCGTCGAGCA GCAGTTTTGC AATCAGCAGT ACAATACCGG CGAGTGGTCC AAGTGGACTG 
GAAATGAACC GCAGGATGGG CGCCATCCTA GAGACCGCCC TGCAACGGGC ACCCATAGTG 
CTTATTAAGA CGGAAAAGCT TCAAGAGGCC GTTGAACGGT ATCGAATCAT GCTAAACGCC 
ATCGAAACGA GGGCTACTCA ATCGTTGCGC CTCACGCTGG CCCGCCAGCT AGCTGAGGTT 
CTTTTGAGAG GGGTCTCGGG CACGATTTAC TCGCCTCCTT TTACCGGAAA ATCTGGAGGT 
GGGACGCTGC GAGGAGGATC CTCCAAGAAA CTTTGGAAAC CGCGTAAATA CGCAGCCCGC 
CAGCAGTTTA ACCCTCGGAA CCAGCAGGAA GAGGTAATTC TGTTGCTACT CATAGCCGAG 
GCACTGGCTG TGAGGGATAC AGTTTTGTCG CAGAGTCCAG AGTTTAGACA GGCCCGTCAG 
CATGCTATGG GCAACGTTAC GGCCGTCTAT GACCTCCTGA CGTTGGCTAC TGTGCGCTGG 
GGGCTCGTCC AGCTATTAAA TGAGTCCTTT GAGAAGGCGC TAAAGTTTAG CTTTGGTGAA 
CAGCATGTGT GGCGGCAGTA CGGCTTAAGT TTGATGGCAG CCGAAAAGCA CTCGCACGCT 
TTAAGGGTCT TGCAAGAATC AATGAAGTTG ACTCCTAGTG ATCCTTTGCC ATGTCTGTTG 
GCTTCTCGCC TTTGCTACGA GAGTCTGGAG ACGGTAAAGC AAGGTCTGGA CTATGCTCAG 
CAAGCGCTGA AGCGCGAAGT AAAGGGCTTG CGACCATCGC GAAGCCAACT CTTTGTGGGC 
ATCGGTCACC AACAGCTAGC CATCCAGTCA AATCTTAAAA GCGAGCGAGA TGCTTGTCAC 
AAGCTGGCTT TGGACGCCCT GGAGCGCGCT GTGCAGTTTG ATGGGAACGA CCACCTGGCG 
GAATACTACT TGTCGTTGCA GTACGCACTT CTGGGACAGC TGGCGGAGGC ATTGGTTCAT 
ATCCGTTTCG CGCTGGCGTT GCGTATGGAA CATGCGCCAT GTCTACACCT GTTCGCACTG 
TTGCTGACAT CGTCGCGCCG ACCTCGTGAA GCTTTGGGAG TTGTTGAGGA TGCTTTACAC 
GAGTTTCCCG ATAACCTGCA GCTACTGCAC GTTAAGGCAC ATCTTCAGCT GCATCTAGAG 
GACGCGGAGA CGGCGTTGGG CACTGTGCAG CACATGCTGG CCGTGTGGCG GGACGTTTAC 
GAGGCCCAGC TAGCGGGAGA GGAGGAAAAG CACTCAGACA CCAAGAGTGG TGTTCACTTG 
GCACATTCCT CACAGATGTC CGACAAGGAT TCAATCTCCC GCGTTGAACA GGCTCTGAGT 
GAAGCAGCAA GCTCATTGAG CTCATTTACG CAGCGTCCTG GACCCCGACG ACCCTGGATG 
CTACAGATTG AAATATGGCT TCTGCTGGCT GATGTCTATC TGCGGATTGA TCAGCCGAAC 
GAGGCACTCA ACTGCATACA CGAAGCCTCA CAGATTTATC CGCTTTCGCA TCAGATTATG 
TTTATGCGTG GCCAGGTGCA TGTCTATTTG GAGCAATGGT TTGACGCCAA GCAATGTTTC 
CTGAACGCCG TGGCCGCCAA CCCAAATCAC ACAGAGGCTT TGCGTGCGCT TGGAGAGGCG 
CATTTGGTAC TGGGCGAGCC GAGGTTGGCT GAAAAAATGC TAAAAGATGC GGCCAAACTG 
GATCCGAGCT GTCCAAAAAT TTGGTTCGCA CTGGGAAAGG TGATGGAGAT CCTGGGCGAT 
TTCCATGCCT CAGCCGATTG CTTCGCCACG TCGCTGCAGT TAGAGCCATC ATGTCCGGTG 
CTACCTTTTA CTTCTATACC TTTGGTGTTT GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246147.1
CDS	182..2737
Translation	MANKNMRNTTTKVEALIESCRSEGKWHRVIELTDELKTGSPHNECLANFLVGEARLESYLEENALASDSNFGRAKSGLAEARRFLHLALGESGQKAGIALDAYLLLAKLCFACGEYEQSLDNFVKAELNTLAEKELTLRSLKILAESYAIKGLCLEQQTTKPSSKFKKAEKDTEMISCFERASDLGLLYLQEYDLVSGSSGSSNNSTAGSTLNVNASTVQPSSSSFAISSTIPASGPSGLEMNRRMGAILETALQRAPIVLIKTEKLQEAVERYRIMLNAIETRATQSLRLTLARQLAEVLLRGVSGTIYSPPFTGKSGGGTLRGGSSKKLWKPRKYAARQQFNPRNQQEEVILLLLIAEALAVRDTVLSQSPEFRQARQHAMGNVTAVYDLLTLATVRWGLVQLLNESFEKALKFSFGEQHVWRQYGLSLMAAEKHSHALRVLQESMKLTPSDPLPCLLASRLCYESLETVKQGLDYAQQALKREVKGLRPSRSQLFVGIGHQQLAIQSNLKSERDACHKLALDALERAVQFDGNDHLAEYYLSLQYALLGQLAEALVHIRFALALRMEHAPCLHLFALLLTSSRRPREALGVVEDALHEFPDNLQLLHVKAHLQLHLEDAETALGTVQHMLAVWRDVYEAQLAGEEEKHSDTKSGVHLAHSSQMSDKDSISRVEQALSEAASSLSSFTQRPGPRRPWMLQIEIWLLLADVYLRIDQPNEALNCIHEASQIYPLSHQIMFMRGQVHVYLEQWFDAKQCFLNAVAANPNHTEALRALGEAHLVLGEPRLAEKMLKDAAKLDPSCPKIWFALGKVMEILGDFHASADCFATSLQLEPSCPVLPFTSIPLVFE

Target ORF information:

RefSeq Version	NM_001259218.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tetratricopeptide repeat domain 7, transcript variant B (Ttc7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259218.2

ORF Insert Sequence:

ATGGCCAACA AGAACATGCG TAACACCACA ACCAAAGTGG AGGCCCTGAT TGAGAGCTGT 
CGCAGCGAGG GCAAGTGGCA CCGGGTCATC GAGCTAACGG ATGAACTGAA GACCGGGTCC 
CCGCACAATG AGTGCCTGGC CAACTTTCTG GTGGGGGAAG CTCGTCTGGA GAGTTACCTG 
GAGGAAAACG CTCTCGCGTC AGACTCAAAT TTTGGTCGCG CCAAGTCTGG ATTGGCGGAG 
GCCCGGCGTT TCCTTCACTT GGCTTTGGGT GAGAGTGGCC AGAAGGCGGG CATCGCCCTG 
GACGCCTATT TGCTGCTGGC CAAGCTGTGC TTTGCCTGCG GCGAGTATGA GCAGAGTCTA 
GACAATTTCG TCAAGGCAGA ACTCAACACG CTTGCCGAGA AGGAGCTGAC CCTTCGCAGC 
CTGAAGATCC TTGCGGAATC GTATGCCATC AAGGGATTGT GTCTGGAGCA GCAGACTACG 
AAGCCGTCAT CTAAGTTTAA GAAGGCCGAA AAGGACACGG AAATGATTAG CTGTTTTGAG 
CGCGCATCTG ATCTGGGGCT GCTCTATCTG CAGGAATACG ATCTCGTTAG TGGAAGCAGT 
GGCTCGTCCA ACAACTCGAC AGCTGGTTCT ACGTTGAATG TAAACGCCTC TACTGTGCAG 
CCGTCGAGCA GCAGTTTTGC AATCAGCAGT ACAATACCGG CGAGTGGTCC AAGTGGACTG 
GAAATGAACC GCAGGATGGG CGCCATCCTA GAGACCGCCC TGCAACGGGC ACCCATAGTG 
CTTATTAAGA CGGAAAAGCT TCAAGAGGCC GTTGAACGGT ATCGAATCAT GCTAAACGCC 
ATCGAAACGA GGGCTACTCA ATCGTTGCGC CTCACGCTGG CCCGCCAGCT AGCTGAGGTT 
CTTTTGAGAG GGGTCTCGGG CACGATTTAC TCGCCTCCTT TTACCGGAAA ATCTGGAGGT 
GGGACGCTGC GAGGAGGATC CTCCAAGAAA CTTTGGAAAC CGCGTAAATA CGCAGCCCGC 
CAGCAGTTTA ACCCTCGGAA CCAGCAGGAA GAGGTAATTC TGTTGCTACT CATAGCCGAG 
GCACTGGCTG TGAGGGATAC AGTTTTGTCG CAGAGTCCAG AGTTTAGACA GGCCCGTCAG 
CATGCTATGG GCAACGTTAC GGCCGTCTAT GACCTCCTGA CGTTGGCTAC TGTGCGCTGG 
GGGCTCGTCC AGCTATTAAA TGAGTCCTTT GAGAAGGCGC TAAAGTTTAG CTTTGGTGAA 
CAGCATGTGT GGCGGCAGTA CGGCTTAAGT TTGATGGCAG CCGAAAAGCA CTCGCACGCT 
TTAAGGGTCT TGCAAGAATC AATGAAGTTG ACTCCTAGTG ATCCTTTGCC ATGTCTGTTG 
GCTTCTCGCC TTTGCTACGA GAGTCTGGAG ACGGTAAAGC AAGGTCTGGA CTATGCTCAG 
CAAGCGCTGA AGCGCGAAGT AAAGGGCTTG CGACCATCGC GAAGCCAACT CTTTGTGGGC 
ATCGGTCACC AACAGCTAGC CATCCAGTCA AATCTTAAAA GCGAGCGAGA TGCTTGTCAC 
AAGCTGGCTT TGGACGCCCT GGAGCGCGCT GTGCAGTTTG ATGGGAACGA CCACCTGGCG 
GAATACTACT TGTCGTTGCA GTACGCACTT CTGGGACAGC TGGCGGAGGC ATTGGTTCAT 
ATCCGTTTCG CGCTGGCGTT GCGTATGGAA CATGCGCCAT GTCTACACCT GTTCGCACTG 
TTGCTGACAT CGTCGCGCCG ACCTCGTGAA GCTTTGGGAG TTGTTGAGGA TGCTTTACAC 
GAGTTTCCCG ATAACCTGCA GCTACTGCAC GTTAAGGCAC ATCTTCAGCT GCATCTAGAG 
GACGCGGAGA CGGCGTTGGG CACTGTGCAG CACATGCTGG CCGTGTGGCG GGACGTTTAC 
GAGGCCCAGC TAGCGGGAGA GGAGGAAAAG CACTCAGACA CCAAGAGTGG TGTTCACTTG 
GCACATTCCT CACAGATGTC CGACAAGGAT TCAATCTCCC GCGTTGAACA GGCTCTGAGT 
GAAGCAGCAA GCTCATTGAG CTCATTTACG CAGCGTCCTG GACCCCGACG ACCCTGGATG 
CTACAGATTG AAATATGGCT TCTGCTGGCT GATGTCTATC TGCGGATTGA TCAGCCGAAC 
GAGGCACTCA ACTGCATACA CGAAGCCTCA CAGATTTATC CGCTTTCGCA TCAGATTATG 
TTTATGCGTG GCCAGGTGCA TGTCTATTTG GAGCAATGGT TTGACGCCAA GCAATGTTTC 
CTGAACGCCG TGGCCGCCAA CCCAAATCAC ACAGAGGCTT TGCGTGCGCT TGGAGAGGCG 
CATTTGGTAC TGGGCGAGCC GAGGTTGGCT GAAAAAATGC TAAAAGATGC GGCCAAACTG 
GATCCGAGCT GTCCAAAAAT TTGGTTCGCA CTGGGAAAGG TGATGGAGAT CCTGGGCGAT 
TTCCATGCCT CAGCCGATTG CTTCGCCACG TCGCTGCAGT TAGAGCCATC ATGTCCGGTG 
CTACCTTTTA CTTCTATACC TTTGGTGTTT GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ttc7 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

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ORF Insert Sequence:

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