Vhl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vhl
Entrez Gene ID	53433
Full Name	von Hippel-Lindau
Synonyms	BcDNA:RH61560,CG13221,DVhl,Dmel\CG13221,Dvhl,VHL,d-VHL,d-vhl,dVHL,dmVHL,vhl
General protein information	Preferred Names von Hippel-Lindau Names CG13221 gene product from transcript CG13221-RA CG13221-PA CG13221-PB Vhl-PA Vhl-PB von hippel lindau von hippel lindau protein
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 47E5-47E6

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273956.1	NP_001260885.1	von Hippel-Lindau, isoform B
NM_080247.3	NP_524986.1	von Hippel-Lindau, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Interaction between Nm23 and the tumor suppressor VHL
Title: Interaction between Nm23 and the tumor suppressor VHL.

Transgenic pVHL can fully maintain normal dVHL-HIF1-alpha downstream pathways in flies.
Title: Computational and experimental characterization of dVHL establish a Drosophila model of VHL syndrome.

Codepletion of Vhl with Mgr gives partial rescue of tubulin instability, monopolar spindle formation, and loss of centrosomes.
Title: Drosophila Mgr, a Prefoldin subunit cooperating with von Hippel Lindau to regulate tubulin stability.

The results establish a developmental function of the VHL gene that is relevant to its tumor-suppressor activity.
Title: Drosophila VHL tumor-suppressor gene regulates epithelial morphogenesis by promoting microtubule and aPKC stability.

findings reveal a second type of tracheal hypoxic response in which Sima activation conflicts with developmental tracheogenesis, and identify the dVHL and ago ubiquitin ligases as key determinants of hypoxia sensitivity in tracheal cells
Title: Regulation of Drosophila embryonic tracheogenesis by dVHL and hypoxia.

The following Vhl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vhl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11526	NM_080247.3 Latest version!	Drosophila melanogaster von Hippel-Lindau (Vhl), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa11526	NM_001273956.1 Latest version!	Drosophila melanogaster von Hippel-Lindau (Vhl), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vhl ( NM_001273956.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260885.1 Drosophila melanogaster von Hippel-Lindau (Vhl), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa11526
Clone ID Related Accession (Same CDS sequence)	NM_001273956.1 , NM_080247.3
Accession Version	NM_001273956.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	von Hippel-Lindau, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCTCC AAATAGCGCA GAACAACCGC GACGGCCAGC AGCTGGTGGG CGCTGATCAG 
GGAAAGGTGG AGGTGTACGT GCTGTTTGCA AACACCACCT ATCGCACCCT GGATCTGTAC 
TGGGTGTGCG AGCGGGAGCG GGAGAACATG TACCTTACCC TCAAGCCCTT CGAGGAGGTG 
CGGGTGAACA CGTTCACCAC ACACAGCTGG CTGTTCCGGG ATTACTACAC GGGCGAACGG 
ATGCACGTAC GCTCGCAGAG GATCTTTCAG CCGATTCGGG TGCGGGTACC CAAGAGCCAG 
CAGAGTCCGG ATCAGCTGGT TGACGTACGC AGCGAAGTGC TGATCCACTT TCCGATGAGA 
TCGCTGCGCG AAAACTGCCT CTGGCTGGTC GCCCGCTGGC TGATCCGGAC GAGCAACGCG 
CCACGGAGGA TTATCCACGG ATACCACATA CCCTCGACGC TGAAGCAACA GCTGCTCAGC 
CTGCTGACCT GCATCGAGTC CTATTCCCGA GTGGCGGGCA CGCGACGTCG GCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260885.1
CDS	154..690
Translation	MALQIAQNNRDGQQLVGADQGKVEVYVLFANTTYRTLDLYWVCERERENMYLTLKPFEEVRVNTFTTHSWLFRDYYTGERMHVRSQRIFQPIRVRVPKSQQSPDQLVDVRSEVLIHFPMRSLRENCLWLVARWLIRTSNAPRRIIHGYHIPSTLKQQLLSLLTCIESYSRVAGTRRRR

Target ORF information:

RefSeq Version	NM_001273956.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster von Hippel-Lindau (Vhl), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273956.1

ORF Insert Sequence:

ATGGCGCTCC AAATAGCGCA GAACAACCGC GACGGCCAGC AGCTGGTGGG CGCTGATCAG 
GGAAAGGTGG AGGTGTACGT GCTGTTTGCA AACACCACCT ATCGCACCCT GGATCTGTAC 
TGGGTGTGCG AGCGGGAGCG GGAGAACATG TACCTTACCC TCAAGCCCTT CGAGGAGGTG 
CGGGTGAACA CGTTCACCAC ACACAGCTGG CTGTTCCGGG ATTACTACAC GGGCGAACGG 
ATGCACGTAC GCTCGCAGAG GATCTTTCAG CCGATTCGGG TGCGGGTACC CAAGAGCCAG 
CAGAGTCCGG ATCAGCTGGT TGACGTACGC AGCGAAGTGC TGATCCACTT TCCGATGAGA 
TCGCTGCGCG AAAACTGCCT CTGGCTGGTC GCCCGCTGGC TGATCCGGAC GAGCAACGCG 
CCACGGAGGA TTATCCACGG ATACCACATA CCCTCGACGC TGAAGCAACA GCTGCTCAGC 
CTGCTGACCT GCATCGAGTC CTATTCCCGA GTGGCGGGCA CGCGACGTCG GCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vhl ( NM_080247.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524986.1 Drosophila melanogaster von Hippel-Lindau (Vhl), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa11526
Clone ID Related Accession (Same CDS sequence)	NM_001273956.1 , NM_080247.3
Accession Version	NM_080247.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	von Hippel-Lindau, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_080247.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCTCC AAATAGCGCA GAACAACCGC GACGGCCAGC AGCTGGTGGG CGCTGATCAG 
GGAAAGGTGG AGGTGTACGT GCTGTTTGCA AACACCACCT ATCGCACCCT GGATCTGTAC 
TGGGTGTGCG AGCGGGAGCG GGAGAACATG TACCTTACCC TCAAGCCCTT CGAGGAGGTG 
CGGGTGAACA CGTTCACCAC ACACAGCTGG CTGTTCCGGG ATTACTACAC GGGCGAACGG 
ATGCACGTAC GCTCGCAGAG GATCTTTCAG CCGATTCGGG TGCGGGTACC CAAGAGCCAG 
CAGAGTCCGG ATCAGCTGGT TGACGTACGC AGCGAAGTGC TGATCCACTT TCCGATGAGA 
TCGCTGCGCG AAAACTGCCT CTGGCTGGTC GCCCGCTGGC TGATCCGGAC GAGCAACGCG 
CCACGGAGGA TTATCCACGG ATACCACATA CCCTCGACGC TGAAGCAACA GCTGCTCAGC 
CTGCTGACCT GCATCGAGTC CTATTCCCGA GTGGCGGGCA CGCGACGTCG GCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524986.1
CDS	154..690
Translation	MALQIAQNNRDGQQLVGADQGKVEVYVLFANTTYRTLDLYWVCERERENMYLTLKPFEEVRVNTFTTHSWLFRDYYTGERMHVRSQRIFQPIRVRVPKSQQSPDQLVDVRSEVLIHFPMRSLRENCLWLVARWLIRTSNAPRRIIHGYHIPSTLKQQLLSLLTCIESYSRVAGTRRRR

Target ORF information:

RefSeq Version	NM_080247.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster von Hippel-Lindau (Vhl), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080247.3

ORF Insert Sequence:

ATGGCGCTCC AAATAGCGCA GAACAACCGC GACGGCCAGC AGCTGGTGGG CGCTGATCAG 
GGAAAGGTGG AGGTGTACGT GCTGTTTGCA AACACCACCT ATCGCACCCT GGATCTGTAC 
TGGGTGTGCG AGCGGGAGCG GGAGAACATG TACCTTACCC TCAAGCCCTT CGAGGAGGTG 
CGGGTGAACA CGTTCACCAC ACACAGCTGG CTGTTCCGGG ATTACTACAC GGGCGAACGG 
ATGCACGTAC GCTCGCAGAG GATCTTTCAG CCGATTCGGG TGCGGGTACC CAAGAGCCAG 
CAGAGTCCGG ATCAGCTGGT TGACGTACGC AGCGAAGTGC TGATCCACTT TCCGATGAGA 
TCGCTGCGCG AAAACTGCCT CTGGCTGGTC GCCCGCTGGC TGATCCGGAC GAGCAACGCG 
CCACGGAGGA TTATCCACGG ATACCACATA CCCTCGACGC TGAAGCAACA GCTGCTCAGC 
CTGCTGACCT GCATCGAGTC CTATTCCCGA GTGGCGGGCA CGCGACGTCG GCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Vhl cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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