CHERP cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHERP
Entrez Gene ID	10523
Full Name	calcium homeostasis endoplasmic reticulum protein
Synonyms	DAN16,SCAF6,SRA1
General protein information	Preferred Names calcium homeostasis endoplasmic reticulum protein Names calcium homeostasis endoplasmic reticulum protein ERPROT 213-21 ERPROT213-21 SR-related CTD associated factor 6 protein with polyglutamine repeat
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.11

mRNA and Protein(s)

mRNA	Protein	Name
NM_006387.6	NP_006378.3	calcium homeostasis endoplasmic reticulum protein
NM_006387.5	NP_006378.3	calcium homeostasis endoplasmic reticulum protein

Pathways from BioSystems

Homologies

	CHERP	XP_001112800.1
Gallus gallus (chicken)	CHERP	NP_001006341.2
Bos taurus (cattle)	CHERP	NP_001103268.1
Mus musculus (house mouse)	Cherp	NP_613051.3
Xenopus tropicalis (tropical clawed frog)	cherp	NP_001011099.1
Rattus norvegicus (Norway rat)	Cherp	NP_001099534.1
Danio rerio (zebrafish)	cherp	NP_956368.2
Homo sapiens (human)	CHERP	NP_006378.3
Pan troglodytes (chimpanzee)	CHERP	XP_001172855.3
Canis lupus familiaris (dog)	CHERP	XP_852773.2

Gene Ontology
Bibliography

Related articles in PubMed

Mammalian splicing factor SF1 interacts with SURP domains of U2 snRNP-associated proteins.
Crisci A, Raleff F, Bagdiul I, Raabe M, Urlaub H, Rain JC, Kr?mer A
Nucleic acids research43(21)10456-73(2015 Dec)

Nuclear ALG-2 protein interacts with Ca2+ homeostasis endoplasmic reticulum protein (CHERP) Ca2+-dependently and participates in regulation of alternative splicing of inositol trisphosphate receptor type 1 (IP3R1) pre-mRNA.
Sasaki-Osugi K, Imoto C, Takahara T, Shibata H, Maki M
The Journal of biological chemistry288(46)33361-75(2013 Nov)

Re-evaluation of the role of calcium homeostasis endoplasmic reticulum protein (CHERP) in cellular calcium signaling.
Lin-Moshier Y, Sebastian PJ, Higgins L, Sampson ND, Hewitt JE, Marchant JS
The Journal of biological chemistry288(1)355-67(2013 Jan)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CHERP and ALG-2 participate in regulation of alternative splicing of IP3R1 pre-mRNA and provide new insights into post-transcriptional regulation of splicing variants in Ca(2+) signaling pathways.
Title: Nuclear ALG-2 protein interacts with Ca2+ homeostasis endoplasmic reticulum protein (CHERP) Ca2+-dependently and participates in regulation of alternative splicing of inositol trisphosphate receptor type 1 (IP3R1) pre-mRNA.

analysis of the role of calcium homeostasis endoplasmic reticulum protein (CHERP) in cellular calcium signaling
Title: Re-evaluation of the role of calcium homeostasis endoplasmic reticulum protein (CHERP) in cellular calcium signaling.

CHERP is an RyR1 interacting protein that may be involved in the regulation of excitation-contraction coupling
Title: Identification of novel ryanodine receptor 1 (RyR1) protein interaction with calcium homeostasis endoplasmic reticulum protein (CHERP).

The following CHERP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHERP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu05387	NM_006387.6 Latest version!	Homo sapiens calcium homeostasis endoplasmic reticulum protein (CHERP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
View Old Accession Versions >>
OHu05387	NM_006387.5	Homo sapiens calcium homeostasis endoplasmic reticulum protein (CHERP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHERP ( NM_006387.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_006378.3 Homo sapiens calcium homeostasis endoplasmic reticulum protein (CHERP), mRNA.

Price & Availability↑

CloneID	OHu05387
Clone ID Related Accession (Same CDS sequence)	NM_006387.6 , NM_006387.5
Accession Version	NM_006387.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	calcium homeostasis endoplasmic reticulum protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC021294.2, U94836.2 and AA782418.1. On Nov 22, 2018 this sequence version replaced NM_006387.5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC021294.2, AF536542.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000546361.7/ ENSP00000439856.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGATGC CGCTGCCCCC CGATGACCAG GAGCTTCGAA ATGTCATCGA CAAGCTCGCC 
CAGTTCGTGG CTCGCAATGG GCCCGAGTTT GAGAAGATGA CTATGGAGAA GCAGAAGGAC 
AACCCCAAAT TCTCGTTTCT TTTCGGAGGC GAATTCTACA GTTACTACAA GTGCAAGCTG 
GCGCTGGAGC AGCAGCAGCT CATCTGCAAG CAGCAGACCC CGGAGCTGGA GCCAGCCGCC 
ACCATGCCAC CCCTGCCACA GCCCCCGCTG GCCCCCGCCG CGCCCATCCC GCCGGCCCAG 
GGCGCGCCAT CCATGGACGA GCTCATCCAG CAGAGCCAGT GGAACCTCCA GCAGCAGGAG 
CAGCACTTGC TGGCGCTCAG ACAGGAGCAA GTGACAGCGG CCGTGGCCCA CGCGGTGGAG 
CAGCAGATGC AGAAGCTTCT GGAGGAGACC CAGCTAGACA TGAACGAGTT TGACAACCTC 
CTGCAGCCCA TCATCGACAC GTGCACCAAG GACGCCATCT CGGCCGGGAA GAACTGGATG 
TTCAGCAATG CCAAGTCCCC GCCGCACTGT GAGCTGATGG CCGGCCACCT CCGGAACCGC 
ATCACGGCTG ATGGGGCACA CTTCGAGCTG CGGCTGCACC TCATCTACCT GATCAATGAC 
GTGCTGCACC ACTGCCAGCG CAAGCAGGCC CGGGAGCTGC TGGCCGCCCT GCAGAAGGTC 
GTGGTGCCCA TCTACTGCAC CAGCTTCTTG GCCGTGGAGG AAGACAAGCA GCAGAAGATC 
GCCCGGCTCC TGCAGCTCTG GGAGAAAAAC GGCTACTTCG ATGACTCCAT CATTCAGCAG 
CTACAGAGCC CAGCCCTGGG GCTTGGTCAG TACCAGGCCA CCCTCATCAA CGAGTACTCC 
TCAGTGGTCC AGCCGGTGCA GCTGGCCTTC CAGCAGCAGA TCCAGACCCT CAAGACGCAG 
CACGAGGAGT TTGTCACCAG CCTGGCCCAG CAGCAGCAGC AGCAGCAACA GCAGCAGCAG 
CAGCTCCAGA TGCCGCAGAT GGAGGCTGAA GTCAAGGCCA CGCCTCCACC GCCTGCTCCA 
CCCCCGGCCC CAGCACCTGC CCCTGCCATC CCGCCCACCA CCCAGCCTGA TGACAGCAAG 
CCTCCCATCC AGATGCCTGG CTCTTCAGAG TACGAAGCTC CAGGAGGGGT CCAGGATCCT 
GCAGCTGCCG GCCCCCGGGG CCCCGGGCCA CACGACCAGA TCCCACCAAA CAAGCCCCCT 
TGGTTTGACC AGCCTCACCC CGTGGCTCCT TGGGGCCAGC AGCAGCCGCC AGAGCAGCCA 
CCCTACCCGC ACCACCAGGG CGGCCCACCC CACTGCCCCC CCTGGAACAA CAGCCATGAG 
GGCATGTGGG GCGAGCAGCG CGGTGACCCC GGCTGGAACG GCCAGCGCGA CGCGCCCTGG 
AACAACCAGC CCGACGCCGC CTGGAACAGC CAGTTCGAGG GCCCCTGGAA CAGCCAGCAC 
GAGCAGCCGC CCTGGGGCGG GGGCCAGCGC GAGCCACCCT TCCGCATGCA GCGGCCCCCA 
CACTTCCGGG GGCCCTTCCC GCCCCACCAG CAGCACCCGC AGTTCAACCA GCCTCCGCAC 
CCCCACAACT TCAACCGCTT CCCGCCCCGC TTCATGCAGG ACGACTTCCC GCCACGGCAC 
CCCTTCGAGC GGCCGCCCTA TCCCCACCGC TTCGACTACC CCCAGGGGGA CTTCCCTGCC 
GAAATGGGGC CCCCTCACCA CCACCCTGGC CACCGCATGC CTCATCCTGG CATCAACGAG 
CACCCGCCTT GGGCTGGACC CCAGCACCCT GACTTCGGCC CTCCCCCCCA TGGCTTCAAC 
GGGCAGCCCC CACACATGCG GCGACAGGGC CCACCCCACA TCAACCACGA TGACCCCAGC 
CTGGTCCCCA ATGTGCCCTA CTTCGATCTC CCTGCTGGGC TGATGGCCCC CCTCGTGAAG 
CTGGAAGATC ACGAGTACAA GCCTTTGGAC CCTAAAGACA TCCGCCTCCC ACCCCCCATG 
CCGCCCAGCG AGAGGCTGCT GGCTGCAGTG GAGGCCTTCT ACAGCCCCCC GTCCCACGAC 
AGGCCCAGGA ACAGTGAAGG CTGGGAGCAG AACGGCCTCT ATGAGTTCTT CCGAGCAAAA 
ATGCGGGCCC GGCGGAGGAA AGGCCAGGAG AAGAGGAACA GCGGACCCTC GAGGTCTCGG 
AGCAGATCCA AGAGTCGAGG GCGTTCTTCC TCCCGCTCCA ACTCAAGATC CTCCAAGTCT 
TCAGGCTCGT ACTCAAGGTC AAGGTCGCGC TCCTGCTCCC GTTCCTACTC CCGCTCCAGA 
TCTAGAAGTC GGAGCAGGTC GCGCTCCTCC AGAAGCCGCT CCCGGTCCCA GTCGCGGTCC 
CGGTCCAAGT CGTACTCCCC AGGAAGAAGA CGCCGGTCAC GGTCCAGGAG CCCCACCCCG 
CCTTCCTCTG CTGGTCTGGG TTCTAATTCG GCGCCTCCCA TCCCTGACTC AAGGCTCGGA 
GAAGAGAACA AAGGCCATCA GATGCTGGTG AAGATGGGCT GGAGCGGCTC AGGCGGCCTC 
GGTGCGAAGG AGCAAGGGAT CCAGGACCCC ATCAAGGGCG GGGACGTCCG GGATAAGTGG 
GACCAGTATA AAGGCGTGGG CGTGGCTCTG GATGACCCCT ATGAGAACTA CCGCAGGAAC 
AAGAGCTACT CCTTCATCGC CCGCATGAAG GCCAGGGACG AGTGTAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006378.3
CDS	60..2810
Translation	MEMPLPPDDQELRNVIDKLAQFVARNGPEFEKMTMEKQKDNPKFSFLFGGEFYSYYKCKLALEQQQLICKQQTPELEPAATMPPLPQPPLAPAAPIPPAQGAPSMDELIQQSQWNLQQQEQHLLALRQEQVTAAVAHAVEQQMQKLLEETQLDMNEFDNLLQPIIDTCTKDAISAGKNWMFSNAKSPPHCELMAGHLRNRITADGAHFELRLHLIYLINDVLHHCQRKQARELLAALQKVVVPIYCTSFLAVEEDKQQKIARLLQLWEKNGYFDDSIIQQLQSPALGLGQYQATLINEYSSVVQPVQLAFQQQIQTLKTQHEEFVTSLAQQQQQQQQQQQQLQMPQMEAEVKATPPPPAPPPAPAPAPAIPPTTQPDDSKPPIQMPGSSEYEAPGGVQDPAAAGPRGPGPHDQIPPNKPPWFDQPHPVAPWGQQQPPEQPPYPHHQGGPPHCPPWNNSHEGMWGEQRGDPGWNGQRDAPWNNQPDAAWNSQFEGPWNSQHEQPPWGGGQREPPFRMQRPPHFRGPFPPHQQHPQFNQPPHPHNFNRFPPRFMQDDFPPRHPFERPPYPHRFDYPQGDFPAEMGPPHHHPGHRMPHPGINEHPPWAGPQHPDFGPPPHGFNGQPPHMRRQGPPHINHDDPSLVPNVPYFDLPAGLMAPLVKLEDHEYKPLDPKDIRLPPPMPPSERLLAAVEAFYSPPSHDRPRNSEGWEQNGLYEFFRAKMRARRRKGQEKRNSGPSRSRSRSKSRGRSSSRSNSRSSKSSGSYSRSRSRSCSRSYSRSRSRSRSRSRSSRSRSRSQSRSRSKSYSPGRRRRSRSRSPTPPSSAGLGSNSAPPIPDSRLGEENKGHQMLVKMGWSGSGGLGAKEQGIQDPIKGGDVRDKWDQYKGVGVALDDPYENYRRNKSYSFIARMKARDECK

Target ORF information:

RefSeq Version	NM_006387.6
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium homeostasis endoplasmic reticulum protein (CHERP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006387.6

ORF Insert Sequence:

ATGGAGATGC CGCTGCCCCC CGATGACCAG GAGCTTCGAA ATGTCATCGA CAAGCTCGCC 
CAGTTCGTGG CTCGCAATGG GCCCGAGTTT GAGAAGATGA CTATGGAGAA GCAGAAGGAC 
AACCCCAAAT TCTCGTTTCT TTTCGGAGGC GAATTCTACA GTTACTACAA GTGCAAGCTG 
GCGCTGGAGC AGCAGCAGCT CATCTGCAAG CAGCAGACCC CGGAGCTGGA GCCAGCCGCC 
ACCATGCCAC CCCTGCCACA GCCCCCGCTG GCCCCCGCCG CGCCCATCCC GCCGGCCCAG 
GGCGCGCCAT CCATGGACGA GCTCATCCAG CAGAGCCAGT GGAACCTCCA GCAGCAGGAG 
CAGCACTTGC TGGCGCTCAG ACAGGAGCAA GTGACAGCGG CCGTGGCCCA CGCGGTGGAG 
CAGCAGATGC AGAAGCTTCT GGAGGAGACC CAGCTAGACA TGAACGAGTT TGACAACCTC 
CTGCAGCCCA TCATCGACAC GTGCACCAAG GACGCCATCT CGGCCGGGAA GAACTGGATG 
TTCAGCAATG CCAAGTCCCC GCCGCACTGT GAGCTGATGG CCGGCCACCT CCGGAACCGC 
ATCACGGCTG ATGGGGCACA CTTCGAGCTG CGGCTGCACC TCATCTACCT GATCAATGAC 
GTGCTGCACC ACTGCCAGCG CAAGCAGGCC CGGGAGCTGC TGGCCGCCCT GCAGAAGGTC 
GTGGTGCCCA TCTACTGCAC CAGCTTCTTG GCCGTGGAGG AAGACAAGCA GCAGAAGATC 
GCCCGGCTCC TGCAGCTCTG GGAGAAAAAC GGCTACTTCG ATGACTCCAT CATTCAGCAG 
CTACAGAGCC CAGCCCTGGG GCTTGGTCAG TACCAGGCCA CCCTCATCAA CGAGTACTCC 
TCAGTGGTCC AGCCGGTGCA GCTGGCCTTC CAGCAGCAGA TCCAGACCCT CAAGACGCAG 
CACGAGGAGT TTGTCACCAG CCTGGCCCAG CAGCAGCAGC AGCAGCAACA GCAGCAGCAG 
CAGCTCCAGA TGCCGCAGAT GGAGGCTGAA GTCAAGGCCA CGCCTCCACC GCCTGCTCCA 
CCCCCGGCCC CAGCACCTGC CCCTGCCATC CCGCCCACCA CCCAGCCTGA TGACAGCAAG 
CCTCCCATCC AGATGCCTGG CTCTTCAGAG TACGAAGCTC CAGGAGGGGT CCAGGATCCT 
GCAGCTGCCG GCCCCCGGGG CCCCGGGCCA CACGACCAGA TCCCACCAAA CAAGCCCCCT 
TGGTTTGACC AGCCTCACCC CGTGGCTCCT TGGGGCCAGC AGCAGCCGCC AGAGCAGCCA 
CCCTACCCGC ACCACCAGGG CGGCCCACCC CACTGCCCCC CCTGGAACAA CAGCCATGAG 
GGCATGTGGG GCGAGCAGCG CGGTGACCCC GGCTGGAACG GCCAGCGCGA CGCGCCCTGG 
AACAACCAGC CCGACGCCGC CTGGAACAGC CAGTTCGAGG GCCCCTGGAA CAGCCAGCAC 
GAGCAGCCGC CCTGGGGCGG GGGCCAGCGC GAGCCACCCT TCCGCATGCA GCGGCCCCCA 
CACTTCCGGG GGCCCTTCCC GCCCCACCAG CAGCACCCGC AGTTCAACCA GCCTCCGCAC 
CCCCACAACT TCAACCGCTT CCCGCCCCGC TTCATGCAGG ACGACTTCCC GCCACGGCAC 
CCCTTCGAGC GGCCGCCCTA TCCCCACCGC TTCGACTACC CCCAGGGGGA CTTCCCTGCC 
GAAATGGGGC CCCCTCACCA CCACCCTGGC CACCGCATGC CTCATCCTGG CATCAACGAG 
CACCCGCCTT GGGCTGGACC CCAGCACCCT GACTTCGGCC CTCCCCCCCA TGGCTTCAAC 
GGGCAGCCCC CACACATGCG GCGACAGGGC CCACCCCACA TCAACCACGA TGACCCCAGC 
CTGGTCCCCA ATGTGCCCTA CTTCGATCTC CCTGCTGGGC TGATGGCCCC CCTCGTGAAG 
CTGGAAGATC ACGAGTACAA GCCTTTGGAC CCTAAAGACA TCCGCCTCCC ACCCCCCATG 
CCGCCCAGCG AGAGGCTGCT GGCTGCAGTG GAGGCCTTCT ACAGCCCCCC GTCCCACGAC 
AGGCCCAGGA ACAGTGAAGG CTGGGAGCAG AACGGCCTCT ATGAGTTCTT CCGAGCAAAA 
ATGCGGGCCC GGCGGAGGAA AGGCCAGGAG AAGAGGAACA GCGGACCCTC GAGGTCTCGG 
AGCAGATCCA AGAGTCGAGG GCGTTCTTCC TCCCGCTCCA ACTCAAGATC CTCCAAGTCT 
TCAGGCTCGT ACTCAAGGTC AAGGTCGCGC TCCTGCTCCC GTTCCTACTC CCGCTCCAGA 
TCTAGAAGTC GGAGCAGGTC GCGCTCCTCC AGAAGCCGCT CCCGGTCCCA GTCGCGGTCC 
CGGTCCAAGT CGTACTCCCC AGGAAGAAGA CGCCGGTCAC GGTCCAGGAG CCCCACCCCG 
CCTTCCTCTG CTGGTCTGGG TTCTAATTCG GCGCCTCCCA TCCCTGACTC AAGGCTCGGA 
GAAGAGAACA AAGGCCATCA GATGCTGGTG AAGATGGGCT GGAGCGGCTC AGGCGGCCTC 
GGTGCGAAGG AGCAAGGGAT CCAGGACCCC ATCAAGGGCG GGGACGTCCG GGATAAGTGG 
GACCAGTATA AAGGCGTGGG CGTGGCTCTG GATGACCCCT ATGAGAACTA CCGCAGGAAC 
AAGAGCTACT CCTTCATCGC CCGCATGAAG GCCAGGGACG AGTGTAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHERP ( NM_006387.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_006378.3 Homo sapiens calcium homeostasis endoplasmic reticulum protein (CHERP), mRNA.

Price & Availability↑

CloneID	OHu05387
Clone ID Related Accession (Same CDS sequence)	NM_006387.6 , NM_006387.5
Accession Version	NM_006387.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	calcium homeostasis endoplasmic reticulum protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CN336425.1, BC021294.2, U94836.2 and AA782418.1. On Dec 9, 2006 this sequence version replaced NM_006387.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC021294.2, AF536542.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGAGATGC CGCTGCCCCC CGATGACCAG GAGCTTCGAA ATGTCATCGA CAAGCTCGCC 
CAGTTCGTGG CTCGCAATGG GCCCGAGTTT GAGAAGATGA CTATGGAGAA GCAGAAGGAC 
AACCCCAAAT TCTCGTTTCT TTTCGGAGGC GAATTCTACA GTTACTACAA GTGCAAGCTG 
GCGCTGGAGC AGCAGCAGCT CATCTGCAAG CAGCAGACCC CGGAGCTGGA GCCAGCCGCC 
ACCATGCCAC CCCTGCCACA GCCCCCGCTG GCCCCCGCCG CGCCCATCCC GCCGGCCCAG 
GGCGCGCCAT CCATGGACGA GCTCATCCAG CAGAGCCAGT GGAACCTCCA GCAGCAGGAG 
CAGCACTTGC TGGCGCTCAG ACAGGAGCAA GTGACAGCGG CCGTGGCCCA CGCGGTGGAG 
CAGCAGATGC AGAAGCTTCT GGAGGAGACC CAGCTAGACA TGAACGAGTT TGACAACCTC 
CTGCAGCCCA TCATCGACAC GTGCACCAAG GACGCCATCT CGGCCGGGAA GAACTGGATG 
TTCAGCAATG CCAAGTCCCC GCCGCACTGT GAGCTGATGG CCGGCCACCT CCGGAACCGC 
ATCACGGCTG ATGGGGCACA CTTCGAGCTG CGGCTGCACC TCATCTACCT GATCAATGAC 
GTGCTGCACC ACTGCCAGCG CAAGCAGGCC CGGGAGCTGC TGGCCGCCCT GCAGAAGGTC 
GTGGTGCCCA TCTACTGCAC CAGCTTCTTG GCCGTGGAGG AAGACAAGCA GCAGAAGATC 
GCCCGGCTCC TGCAGCTCTG GGAGAAAAAC GGCTACTTCG ATGACTCCAT CATTCAGCAG 
CTACAGAGCC CAGCCCTGGG GCTTGGTCAG TACCAGGCCA CCCTCATCAA CGAGTACTCC 
TCAGTGGTCC AGCCGGTGCA GCTGGCCTTC CAGCAGCAGA TCCAGACCCT CAAGACGCAG 
CACGAGGAGT TTGTCACCAG CCTGGCCCAG CAGCAGCAGC AGCAGCAACA GCAGCAGCAG 
CAGCTCCAGA TGCCGCAGAT GGAGGCTGAA GTCAAGGCCA CGCCTCCACC GCCTGCTCCA 
CCCCCGGCCC CAGCACCTGC CCCTGCCATC CCGCCCACCA CCCAGCCTGA TGACAGCAAG 
CCTCCCATCC AGATGCCTGG CTCTTCAGAG TACGAAGCTC CAGGAGGGGT CCAGGATCCT 
GCAGCTGCCG GCCCCCGGGG CCCCGGGCCA CACGACCAGA TCCCACCAAA CAAGCCCCCT 
TGGTTTGACC AGCCTCACCC CGTGGCTCCT TGGGGCCAGC AGCAGCCGCC AGAGCAGCCA 
CCCTACCCGC ACCACCAGGG CGGCCCACCC CACTGCCCCC CCTGGAACAA CAGCCATGAG 
GGCATGTGGG GCGAGCAGCG CGGTGACCCC GGCTGGAACG GCCAGCGCGA CGCGCCCTGG 
AACAACCAGC CCGACGCCGC CTGGAACAGC CAGTTCGAGG GCCCCTGGAA CAGCCAGCAC 
GAGCAGCCGC CCTGGGGCGG GGGCCAGCGC GAGCCACCCT TCCGCATGCA GCGGCCCCCA 
CACTTCCGGG GGCCCTTCCC GCCCCACCAG CAGCACCCGC AGTTCAACCA GCCTCCGCAC 
CCCCACAACT TCAACCGCTT CCCGCCCCGC TTCATGCAGG ACGACTTCCC GCCACGGCAC 
CCCTTCGAGC GGCCGCCCTA TCCCCACCGC TTCGACTACC CCCAGGGGGA CTTCCCTGCC 
GAAATGGGGC CCCCTCACCA CCACCCTGGC CACCGCATGC CTCATCCTGG CATCAACGAG 
CACCCGCCTT GGGCTGGACC CCAGCACCCT GACTTCGGCC CTCCCCCCCA TGGCTTCAAC 
GGGCAGCCCC CACACATGCG GCGACAGGGC CCACCCCACA TCAACCACGA TGACCCCAGC 
CTGGTCCCCA ATGTGCCCTA CTTCGATCTC CCTGCTGGGC TGATGGCCCC CCTCGTGAAG 
CTGGAAGATC ACGAGTACAA GCCTTTGGAC CCTAAAGACA TCCGCCTCCC ACCCCCCATG 
CCGCCCAGCG AGAGGCTGCT GGCTGCAGTG GAGGCCTTCT ACAGCCCCCC GTCCCACGAC 
AGGCCCAGGA ACAGTGAAGG CTGGGAGCAG AACGGCCTCT ATGAGTTCTT CCGAGCAAAA 
ATGCGGGCCC GGCGGAGGAA AGGCCAGGAG AAGAGGAACA GCGGACCCTC GAGGTCTCGG 
AGCAGATCCA AGAGTCGAGG GCGTTCTTCC TCCCGCTCCA ACTCAAGATC CTCCAAGTCT 
TCAGGCTCGT ACTCAAGGTC AAGGTCGCGC TCCTGCTCCC GTTCCTACTC CCGCTCCAGA 
TCTAGAAGTC GGAGCAGGTC GCGCTCCTCC AGAAGCCGCT CCCGGTCCCA GTCGCGGTCC 
CGGTCCAAGT CGTACTCCCC AGGAAGAAGA CGCCGGTCAC GGTCCAGGAG CCCCACCCCG 
CCTTCCTCTG CTGGTCTGGG TTCTAATTCG GCGCCTCCCA TCCCTGACTC AAGGCTCGGA 
GAAGAGAACA AAGGCCATCA GATGCTGGTG AAGATGGGCT GGAGCGGCTC AGGCGGCCTC 
GGTGCGAAGG AGCAAGGGAT CCAGGACCCC ATCAAGGGCG GGGACGTCCG GGATAAGTGG 
GACCAGTATA AAGGCGTGGG CGTGGCTCTG GATGACCCCT ATGAGAACTA CCGCAGGAAC 
AAGAGCTACT CCTTCATCGC CCGCATGAAG GCCAGGGACG AGTGTAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006378.3
CDS	75..2825
Translation	MEMPLPPDDQELRNVIDKLAQFVARNGPEFEKMTMEKQKDNPKFSFLFGGEFYSYYKCKLALEQQQLICKQQTPELEPAATMPPLPQPPLAPAAPIPPAQGAPSMDELIQQSQWNLQQQEQHLLALRQEQVTAAVAHAVEQQMQKLLEETQLDMNEFDNLLQPIIDTCTKDAISAGKNWMFSNAKSPPHCELMAGHLRNRITADGAHFELRLHLIYLINDVLHHCQRKQARELLAALQKVVVPIYCTSFLAVEEDKQQKIARLLQLWEKNGYFDDSIIQQLQSPALGLGQYQATLINEYSSVVQPVQLAFQQQIQTLKTQHEEFVTSLAQQQQQQQQQQQQLQMPQMEAEVKATPPPPAPPPAPAPAPAIPPTTQPDDSKPPIQMPGSSEYEAPGGVQDPAAAGPRGPGPHDQIPPNKPPWFDQPHPVAPWGQQQPPEQPPYPHHQGGPPHCPPWNNSHEGMWGEQRGDPGWNGQRDAPWNNQPDAAWNSQFEGPWNSQHEQPPWGGGQREPPFRMQRPPHFRGPFPPHQQHPQFNQPPHPHNFNRFPPRFMQDDFPPRHPFERPPYPHRFDYPQGDFPAEMGPPHHHPGHRMPHPGINEHPPWAGPQHPDFGPPPHGFNGQPPHMRRQGPPHINHDDPSLVPNVPYFDLPAGLMAPLVKLEDHEYKPLDPKDIRLPPPMPPSERLLAAVEAFYSPPSHDRPRNSEGWEQNGLYEFFRAKMRARRRKGQEKRNSGPSRSRSRSKSRGRSSSRSNSRSSKSSGSYSRSRSRSCSRSYSRSRSRSRSRSRSSRSRSRSQSRSRSKSYSPGRRRRSRSRSPTPPSSAGLGSNSAPPIPDSRLGEENKGHQMLVKMGWSGSGGLGAKEQGIQDPIKGGDVRDKWDQYKGVGVALDDPYENYRRNKSYSFIARMKARDECK

Target ORF information:

RefSeq Version	NM_006387.5
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium homeostasis endoplasmic reticulum protein (CHERP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006387.5

ORF Insert Sequence:

ATGGAGATGC CGCTGCCCCC CGATGACCAG GAGCTTCGAA ATGTCATCGA CAAGCTCGCC 
CAGTTCGTGG CTCGCAATGG GCCCGAGTTT GAGAAGATGA CTATGGAGAA GCAGAAGGAC 
AACCCCAAAT TCTCGTTTCT TTTCGGAGGC GAATTCTACA GTTACTACAA GTGCAAGCTG 
GCGCTGGAGC AGCAGCAGCT CATCTGCAAG CAGCAGACCC CGGAGCTGGA GCCAGCCGCC 
ACCATGCCAC CCCTGCCACA GCCCCCGCTG GCCCCCGCCG CGCCCATCCC GCCGGCCCAG 
GGCGCGCCAT CCATGGACGA GCTCATCCAG CAGAGCCAGT GGAACCTCCA GCAGCAGGAG 
CAGCACTTGC TGGCGCTCAG ACAGGAGCAA GTGACAGCGG CCGTGGCCCA CGCGGTGGAG 
CAGCAGATGC AGAAGCTTCT GGAGGAGACC CAGCTAGACA TGAACGAGTT TGACAACCTC 
CTGCAGCCCA TCATCGACAC GTGCACCAAG GACGCCATCT CGGCCGGGAA GAACTGGATG 
TTCAGCAATG CCAAGTCCCC GCCGCACTGT GAGCTGATGG CCGGCCACCT CCGGAACCGC 
ATCACGGCTG ATGGGGCACA CTTCGAGCTG CGGCTGCACC TCATCTACCT GATCAATGAC 
GTGCTGCACC ACTGCCAGCG CAAGCAGGCC CGGGAGCTGC TGGCCGCCCT GCAGAAGGTC 
GTGGTGCCCA TCTACTGCAC CAGCTTCTTG GCCGTGGAGG AAGACAAGCA GCAGAAGATC 
GCCCGGCTCC TGCAGCTCTG GGAGAAAAAC GGCTACTTCG ATGACTCCAT CATTCAGCAG 
CTACAGAGCC CAGCCCTGGG GCTTGGTCAG TACCAGGCCA CCCTCATCAA CGAGTACTCC 
TCAGTGGTCC AGCCGGTGCA GCTGGCCTTC CAGCAGCAGA TCCAGACCCT CAAGACGCAG 
CACGAGGAGT TTGTCACCAG CCTGGCCCAG CAGCAGCAGC AGCAGCAACA GCAGCAGCAG 
CAGCTCCAGA TGCCGCAGAT GGAGGCTGAA GTCAAGGCCA CGCCTCCACC GCCTGCTCCA 
CCCCCGGCCC CAGCACCTGC CCCTGCCATC CCGCCCACCA CCCAGCCTGA TGACAGCAAG 
CCTCCCATCC AGATGCCTGG CTCTTCAGAG TACGAAGCTC CAGGAGGGGT CCAGGATCCT 
GCAGCTGCCG GCCCCCGGGG CCCCGGGCCA CACGACCAGA TCCCACCAAA CAAGCCCCCT 
TGGTTTGACC AGCCTCACCC CGTGGCTCCT TGGGGCCAGC AGCAGCCGCC AGAGCAGCCA 
CCCTACCCGC ACCACCAGGG CGGCCCACCC CACTGCCCCC CCTGGAACAA CAGCCATGAG 
GGCATGTGGG GCGAGCAGCG CGGTGACCCC GGCTGGAACG GCCAGCGCGA CGCGCCCTGG 
AACAACCAGC CCGACGCCGC CTGGAACAGC CAGTTCGAGG GCCCCTGGAA CAGCCAGCAC 
GAGCAGCCGC CCTGGGGCGG GGGCCAGCGC GAGCCACCCT TCCGCATGCA GCGGCCCCCA 
CACTTCCGGG GGCCCTTCCC GCCCCACCAG CAGCACCCGC AGTTCAACCA GCCTCCGCAC 
CCCCACAACT TCAACCGCTT CCCGCCCCGC TTCATGCAGG ACGACTTCCC GCCACGGCAC 
CCCTTCGAGC GGCCGCCCTA TCCCCACCGC TTCGACTACC CCCAGGGGGA CTTCCCTGCC 
GAAATGGGGC CCCCTCACCA CCACCCTGGC CACCGCATGC CTCATCCTGG CATCAACGAG 
CACCCGCCTT GGGCTGGACC CCAGCACCCT GACTTCGGCC CTCCCCCCCA TGGCTTCAAC 
GGGCAGCCCC CACACATGCG GCGACAGGGC CCACCCCACA TCAACCACGA TGACCCCAGC 
CTGGTCCCCA ATGTGCCCTA CTTCGATCTC CCTGCTGGGC TGATGGCCCC CCTCGTGAAG 
CTGGAAGATC ACGAGTACAA GCCTTTGGAC CCTAAAGACA TCCGCCTCCC ACCCCCCATG 
CCGCCCAGCG AGAGGCTGCT GGCTGCAGTG GAGGCCTTCT ACAGCCCCCC GTCCCACGAC 
AGGCCCAGGA ACAGTGAAGG CTGGGAGCAG AACGGCCTCT ATGAGTTCTT CCGAGCAAAA 
ATGCGGGCCC GGCGGAGGAA AGGCCAGGAG AAGAGGAACA GCGGACCCTC GAGGTCTCGG 
AGCAGATCCA AGAGTCGAGG GCGTTCTTCC TCCCGCTCCA ACTCAAGATC CTCCAAGTCT 
TCAGGCTCGT ACTCAAGGTC AAGGTCGCGC TCCTGCTCCC GTTCCTACTC CCGCTCCAGA 
TCTAGAAGTC GGAGCAGGTC GCGCTCCTCC AGAAGCCGCT CCCGGTCCCA GTCGCGGTCC 
CGGTCCAAGT CGTACTCCCC AGGAAGAAGA CGCCGGTCAC GGTCCAGGAG CCCCACCCCG 
CCTTCCTCTG CTGGTCTGGG TTCTAATTCG GCGCCTCCCA TCCCTGACTC AAGGCTCGGA 
GAAGAGAACA AAGGCCATCA GATGCTGGTG AAGATGGGCT GGAGCGGCTC AGGCGGCCTC 
GGTGCGAAGG AGCAAGGGAT CCAGGACCCC ATCAAGGGCG GGGACGTCCG GGATAAGTGG 
GACCAGTATA AAGGCGTGGG CGTGGCTCTG GATGACCCCT ATGAGAACTA CCGCAGGAAC 
AAGAGCTACT CCTTCATCGC CCGCATGAAG GCCAGGGACG AGTGTAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mammalian splicing factor SF1 interacts with SURP domains of U2 snRNP-associated proteins.
Nucleic acids research43(21)10456-73(2015 Dec)
Crisci A,Raleff F,Bagdiul I,Raabe M,Urlaub H,Rain JC,Kr?mer A

Nuclear ALG-2 protein interacts with Ca2+ homeostasis endoplasmic reticulum protein (CHERP) Ca2+-dependently and participates in regulation of alternative splicing of inositol trisphosphate receptor type 1 (IP3R1) pre-mRNA.
The Journal of biological chemistry288(46)33361-75(2013 Nov)
Sasaki-Osugi K,Imoto C,Takahara T,Shibata H,Maki M

Re-evaluation of the role of calcium homeostasis endoplasmic reticulum protein (CHERP) in cellular calcium signaling.
The Journal of biological chemistry288(1)355-67(2013 Jan)
Lin-Moshier Y,Sebastian PJ,Higgins L,Sampson ND,Hewitt JE,Marchant JS

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

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CHERP cDNA ORF clone, Homo sapiens(human)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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