A2M cDNA ORF clone, Homo sapiens(human)

The following A2M gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the A2M cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu18488 NM_000014.5
Latest version!
Homo sapiens alpha-2-macroglobulin (A2M), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$519.50-$727.30
$1039.00
OHu102221 NM_001347424.1
Latest version!
Homo sapiens alpha-2-macroglobulin (A2M), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu102390 NM_001347425.1
Latest version!
Homo sapiens alpha-2-macroglobulin (A2M), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu108400 NM_001347423.2
Latest version!
Homo sapiens alpha-2-macroglobulin (A2M), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu108400 NM_000014.6
Latest version!
Homo sapiens alpha-2-macroglobulin (A2M), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu109274 NM_001347424.2
Latest version!
Homo sapiens alpha-2-macroglobulin (A2M), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu109275 NM_001347425.2
Latest version!
Homo sapiens alpha-2-macroglobulin (A2M), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu18488 NM_001347423.1
Latest version!
Homo sapiens alpha-2-macroglobulin (A2M), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu46081 XM_006719056.3
Latest version!
Homo sapiens alpha-2-macroglobulin (A2M), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
View Old Accession Versions >>
OHu46081 XM_006719056.2 Homo sapiens alpha-2-macroglobulin (A2M), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu18488
    Clone ID Related Accession (Same CDS sequence) NM_001347423.1 , NM_000014.5
    Accession Version NM_000014.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4425bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-27
    Organism Homo sapiens(human)
    Product alpha-2-macroglobulin isoform a precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC334781.1, DC402196.1, BC040071.1 and AB209614.2. On Nov 26, 2016 this sequence version replaced NM_000014.4. Transcript Variant: This variant (1) encodes the longest isoform (a). Both variants 1 and 2 encode isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC040071.1, SRR1660809.61793.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGGGAAGA ACAAACTCCT TCATCCAAGT CTGGTTCTTC TCCTCTTGGT CCTCCTGCCC 
    ACAGACGCCT CAGTCTCTGG AAAACCGCAG TATATGGTTC TGGTCCCCTC CCTGCTCCAC
    ACTGAGACCA CTGAGAAGGG CTGTGTCCTT CTGAGCTACC TGAATGAGAC AGTGACTGTA
    AGTGCTTCCT TGGAGTCTGT CAGGGGAAAC AGGAGCCTCT TCACTGACCT GGAGGCGGAG
    AATGACGTAC TCCACTGTGT CGCCTTCGCT GTCCCAAAGT CTTCATCCAA TGAGGAGGTA
    ATGTTCCTCA CTGTCCAAGT GAAAGGACCA ACCCAAGAAT TTAAGAAGCG GACCACAGTG
    ATGGTTAAGA ACGAGGACAG TCTGGTCTTT GTCCAGACAG ACAAATCAAT CTACAAACCA
    GGGCAGACAG TGAAATTTCG TGTTGTCTCC ATGGATGAAA ACTTTCACCC CCTGAATGAG
    TTGATTCCAC TAGTATACAT TCAGGATCCC AAAGGAAATC GCATCGCACA ATGGCAGAGT
    TTCCAGTTAG AGGGTGGCCT CAAGCAATTT TCTTTTCCCC TCTCATCAGA GCCCTTCCAG
    GGCTCCTACA AGGTGGTGGT ACAGAAGAAA TCAGGTGGAA GGACAGAGCA CCCTTTCACC
    GTGGAGGAAT TTGTTCTTCC CAAGTTTGAA GTACAAGTAA CAGTGCCAAA GATAATCACC
    ATCTTGGAAG AAGAGATGAA TGTATCAGTG TGTGGCCTAT ACACATATGG GAAGCCTGTC
    CCTGGACATG TGACTGTGAG CATTTGCAGA AAGTATAGTG ACGCTTCCGA CTGCCACGGT
    GAAGATTCAC AGGCTTTCTG TGAGAAATTC AGTGGACAGC TAAACAGCCA TGGCTGCTTC
    TATCAGCAAG TAAAAACCAA GGTCTTCCAG CTGAAGAGGA AGGAGTATGA AATGAAACTT
    CACACTGAGG CCCAGATCCA AGAAGAAGGA ACAGTGGTGG AATTGACTGG AAGGCAGTCC
    AGTGAAATCA CAAGAACCAT AACCAAACTC TCATTTGTGA AAGTGGACTC ACACTTTCGA
    CAGGGAATTC CCTTCTTTGG GCAGGTGCGC CTAGTAGATG GGAAAGGCGT CCCTATACCA
    AATAAAGTCA TATTCATCAG AGGAAATGAA GCAAACTATT ACTCCAATGC TACCACGGAT
    GAGCATGGCC TTGTACAGTT CTCTATCAAC ACCACCAATG TTATGGGTAC CTCTCTTACT
    GTTAGGGTCA ATTACAAGGA TCGTAGTCCC TGTTACGGCT ACCAGTGGGT GTCAGAAGAA
    CACGAAGAGG CACATCACAC TGCTTATCTT GTGTTCTCCC CAAGCAAGAG CTTTGTCCAC
    CTTGAGCCCA TGTCTCATGA ACTACCCTGT GGCCATACTC AGACAGTCCA GGCACATTAT
    ATTCTGAATG GAGGCACCCT GCTGGGGCTG AAGAAGCTCT CCTTCTATTA TCTGATAATG
    GCAAAGGGAG GCATTGTCCG AACTGGGACT CATGGACTGC TTGTGAAGCA GGAAGACATG
    AAGGGCCATT TTTCCATCTC AATCCCTGTG AAGTCAGACA TTGCTCCTGT CGCTCGGTTG
    CTCATCTATG CTGTTTTACC TACCGGGGAC GTGATTGGGG ATTCTGCAAA ATATGATGTT
    GAAAATTGTC TGGCCAACAA GGTGGATTTG AGCTTCAGCC CATCACAAAG TCTCCCAGCC
    TCACACGCCC ACCTGCGAGT CACAGCGGCT CCTCAGTCCG TCTGCGCCCT CCGTGCTGTG
    GACCAAAGCG TGCTGCTCAT GAAGCCTGAT GCTGAGCTCT CGGCGTCCTC GGTTTACAAC
    CTGCTACCAG AAAAGGACCT CACTGGCTTC CCTGGGCCTT TGAATGACCA GGACGATGAA
    GACTGCATCA ATCGTCATAA TGTCTATATT AATGGAATCA CATATACTCC AGTATCAAGT
    ACAAATGAAA AGGATATGTA CAGCTTCCTA GAGGACATGG GCTTAAAGGC ATTCACCAAC
    TCAAAGATTC GTAAACCCAA AATGTGTCCA CAGCTTCAAC AGTATGAAAT GCATGGACCT
    GAAGGTCTAC GTGTAGGTTT TTATGAGTCA GATGTAATGG GAAGAGGCCA TGCACGCCTG
    GTGCATGTTG AAGAGCCTCA CACGGAGACC GTACGAAAGT ACTTCCCTGA GACATGGATC
    TGGGATTTGG TGGTGGTAAA CTCAGCAGGT GTGGCTGAGG TAGGAGTAAC AGTCCCTGAC
    ACCATCACCG AGTGGAAGGC AGGGGCCTTC TGCCTGTCTG AAGATGCTGG ACTTGGTATC
    TCTTCCACTG CCTCTCTCCG AGCCTTCCAG CCCTTCTTTG TGGAGCTCAC AATGCCTTAC
    TCTGTGATTC GTGGAGAGGC CTTCACACTC AAGGCCACGG TCCTAAACTA CCTTCCCAAA
    TGCATCCGGG TCAGTGTGCA GCTGGAAGCC TCTCCCGCCT TCCTAGCTGT CCCAGTGGAG
    AAGGAACAAG CGCCTCACTG CATCTGTGCA AACGGGCGGC AAACTGTGTC CTGGGCAGTA
    ACCCCAAAGT CATTAGGAAA TGTGAATTTC ACTGTGAGCG CAGAGGCACT AGAGTCTCAA
    GAGCTGTGTG GGACTGAGGT GCCTTCAGTT CCTGAACACG GAAGGAAAGA CACAGTCATC
    AAGCCTCTGT TGGTTGAACC TGAAGGACTA GAGAAGGAAA CAACATTCAA CTCCCTACTT
    TGTCCATCAG GTGGTGAGGT TTCTGAAGAA TTATCCCTGA AACTGCCACC AAATGTGGTA
    GAAGAATCTG CCCGAGCTTC TGTCTCAGTT TTGGGAGACA TATTAGGCTC TGCCATGCAA
    AACACACAAA ATCTTCTCCA GATGCCCTAT GGCTGTGGAG AGCAGAATAT GGTCCTCTTT
    GCTCCTAACA TCTATGTACT GGATTATCTA AATGAAACAC AGCAGCTTAC TCCAGAGATC
    AAGTCCAAGG CCATTGGCTA TCTCAACACT GGTTACCAGA GACAGTTGAA CTACAAACAC
    TATGATGGCT CCTACAGCAC CTTTGGGGAG CGATATGGCA GGAACCAGGG CAACACCTGG
    CTCACAGCCT TTGTTCTGAA GACTTTTGCC CAAGCTCGAG CCTACATCTT CATCGATGAA
    GCACACATTA CCCAAGCCCT CATATGGCTC TCCCAGAGGC AGAAGGACAA TGGCTGTTTC
    AGGAGCTCTG GGTCACTGCT CAACAATGCC ATAAAGGGAG GAGTAGAAGA TGAAGTGACC
    CTCTCCGCCT ATATCACCAT CGCCCTTCTG GAGATTCCTC TCACAGTCAC TCACCCTGTT
    GTCCGCAATG CCCTGTTTTG CCTGGAGTCA GCCTGGAAGA CAGCACAAGA AGGGGACCAT
    GGCAGCCATG TATATACCAA AGCACTGCTG GCCTATGCTT TTGCCCTGGC AGGTAACCAG
    GACAAGAGGA AGGAAGTACT CAAGTCACTT AATGAGGAAG CTGTGAAGAA AGACAACTCT
    GTCCATTGGG AGCGCCCTCA GAAACCCAAG GCACCAGTGG GGCATTTTTA CGAACCCCAG
    GCTCCCTCTG CTGAGGTGGA GATGACATCC TATGTGCTCC TCGCTTATCT CACGGCCCAG
    CCAGCCCCAA CCTCGGAGGA CCTGACCTCT GCAACCAACA TCGTGAAGTG GATCACGAAG
    CAGCAGAATG CCCAGGGCGG TTTCTCCTCC ACCCAGGACA CAGTGGTGGC TCTCCATGCT
    CTGTCCAAAT ATGGAGCAGC CACATTTACC AGGACTGGGA AGGCTGCACA GGTGACTATC
    CAGTCTTCAG GGACATTTTC CAGCAAATTC CAAGTGGACA ACAACAACCG CCTGTTACTG
    CAGCAGGTCT CATTGCCAGA GCTGCCTGGG GAATACAGCA TGAAAGTGAC AGGAGAAGGA
    TGTGTCTACC TCCAGACATC CTTGAAATAC AATATTCTCC CAGAAAAGGA AGAGTTCCCC
    TTTGCTTTAG GAGTGCAGAC TCTGCCTCAA ACTTGTGATG AACCCAAAGC CCACACCAGC
    TTCCAAATCT CCCTAAGTGT CAGTTACACA GGGAGCCGCT CTGCCTCCAA CATGGCGATC
    GTTGATGTGA AGATGGTCTC TGGCTTCATT CCCCTGAAGC CAACAGTGAA AATGCTTGAA
    AGATCTAACC ATGTGAGCCG GACAGAAGTC AGCAGCAACC ATGTCTTGAT TTACCTTGAT
    AAGGTGTCAA ATCAGACACT GAGCTTGTTC TTCACGGTTC TGCAAGATGT CCCAGTAAGA
    GATCTGAAAC CAGCCATAGT GAAAGTCTAT GATTACTACG AGACGGATGA GTTTGCAATT
    GCTGAGTACA ATGCTCCTTG CAGCAAAGAT CTTGGAAATG CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000005.2
    CDS381..4805
    Translation

    Target ORF information:

    RefSeq Version NM_000014.5
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha-2-macroglobulin (A2M), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000014.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGGGAAGA ACAAACTCCT TCATCCAAGT CTGGTTCTTC TCCTCTTGGT CCTCCTGCCC 
    ACAGACGCCT CAGTCTCTGG AAAACCGCAG TATATGGTTC TGGTCCCCTC CCTGCTCCAC
    ACTGAGACCA CTGAGAAGGG CTGTGTCCTT CTGAGCTACC TGAATGAGAC AGTGACTGTA
    AGTGCTTCCT TGGAGTCTGT CAGGGGAAAC AGGAGCCTCT TCACTGACCT GGAGGCGGAG
    AATGACGTAC TCCACTGTGT CGCCTTCGCT GTCCCAAAGT CTTCATCCAA TGAGGAGGTA
    ATGTTCCTCA CTGTCCAAGT GAAAGGACCA ACCCAAGAAT TTAAGAAGCG GACCACAGTG
    ATGGTTAAGA ACGAGGACAG TCTGGTCTTT GTCCAGACAG ACAAATCAAT CTACAAACCA
    GGGCAGACAG TGAAATTTCG TGTTGTCTCC ATGGATGAAA ACTTTCACCC CCTGAATGAG
    TTGATTCCAC TAGTATACAT TCAGGATCCC AAAGGAAATC GCATCGCACA ATGGCAGAGT
    TTCCAGTTAG AGGGTGGCCT CAAGCAATTT TCTTTTCCCC TCTCATCAGA GCCCTTCCAG
    GGCTCCTACA AGGTGGTGGT ACAGAAGAAA TCAGGTGGAA GGACAGAGCA CCCTTTCACC
    GTGGAGGAAT TTGTTCTTCC CAAGTTTGAA GTACAAGTAA CAGTGCCAAA GATAATCACC
    ATCTTGGAAG AAGAGATGAA TGTATCAGTG TGTGGCCTAT ACACATATGG GAAGCCTGTC
    CCTGGACATG TGACTGTGAG CATTTGCAGA AAGTATAGTG ACGCTTCCGA CTGCCACGGT
    GAAGATTCAC AGGCTTTCTG TGAGAAATTC AGTGGACAGC TAAACAGCCA TGGCTGCTTC
    TATCAGCAAG TAAAAACCAA GGTCTTCCAG CTGAAGAGGA AGGAGTATGA AATGAAACTT
    CACACTGAGG CCCAGATCCA AGAAGAAGGA ACAGTGGTGG AATTGACTGG AAGGCAGTCC
    AGTGAAATCA CAAGAACCAT AACCAAACTC TCATTTGTGA AAGTGGACTC ACACTTTCGA
    CAGGGAATTC CCTTCTTTGG GCAGGTGCGC CTAGTAGATG GGAAAGGCGT CCCTATACCA
    AATAAAGTCA TATTCATCAG AGGAAATGAA GCAAACTATT ACTCCAATGC TACCACGGAT
    GAGCATGGCC TTGTACAGTT CTCTATCAAC ACCACCAATG TTATGGGTAC CTCTCTTACT
    GTTAGGGTCA ATTACAAGGA TCGTAGTCCC TGTTACGGCT ACCAGTGGGT GTCAGAAGAA
    CACGAAGAGG CACATCACAC TGCTTATCTT GTGTTCTCCC CAAGCAAGAG CTTTGTCCAC
    CTTGAGCCCA TGTCTCATGA ACTACCCTGT GGCCATACTC AGACAGTCCA GGCACATTAT
    ATTCTGAATG GAGGCACCCT GCTGGGGCTG AAGAAGCTCT CCTTCTATTA TCTGATAATG
    GCAAAGGGAG GCATTGTCCG AACTGGGACT CATGGACTGC TTGTGAAGCA GGAAGACATG
    AAGGGCCATT TTTCCATCTC AATCCCTGTG AAGTCAGACA TTGCTCCTGT CGCTCGGTTG
    CTCATCTATG CTGTTTTACC TACCGGGGAC GTGATTGGGG ATTCTGCAAA ATATGATGTT
    GAAAATTGTC TGGCCAACAA GGTGGATTTG AGCTTCAGCC CATCACAAAG TCTCCCAGCC
    TCACACGCCC ACCTGCGAGT CACAGCGGCT CCTCAGTCCG TCTGCGCCCT CCGTGCTGTG
    GACCAAAGCG TGCTGCTCAT GAAGCCTGAT GCTGAGCTCT CGGCGTCCTC GGTTTACAAC
    CTGCTACCAG AAAAGGACCT CACTGGCTTC CCTGGGCCTT TGAATGACCA GGACGATGAA
    GACTGCATCA ATCGTCATAA TGTCTATATT AATGGAATCA CATATACTCC AGTATCAAGT
    ACAAATGAAA AGGATATGTA CAGCTTCCTA GAGGACATGG GCTTAAAGGC ATTCACCAAC
    TCAAAGATTC GTAAACCCAA AATGTGTCCA CAGCTTCAAC AGTATGAAAT GCATGGACCT
    GAAGGTCTAC GTGTAGGTTT TTATGAGTCA GATGTAATGG GAAGAGGCCA TGCACGCCTG
    GTGCATGTTG AAGAGCCTCA CACGGAGACC GTACGAAAGT ACTTCCCTGA GACATGGATC
    TGGGATTTGG TGGTGGTAAA CTCAGCAGGT GTGGCTGAGG TAGGAGTAAC AGTCCCTGAC
    ACCATCACCG AGTGGAAGGC AGGGGCCTTC TGCCTGTCTG AAGATGCTGG ACTTGGTATC
    TCTTCCACTG CCTCTCTCCG AGCCTTCCAG CCCTTCTTTG TGGAGCTCAC AATGCCTTAC
    TCTGTGATTC GTGGAGAGGC CTTCACACTC AAGGCCACGG TCCTAAACTA CCTTCCCAAA
    TGCATCCGGG TCAGTGTGCA GCTGGAAGCC TCTCCCGCCT TCCTAGCTGT CCCAGTGGAG
    AAGGAACAAG CGCCTCACTG CATCTGTGCA AACGGGCGGC AAACTGTGTC CTGGGCAGTA
    ACCCCAAAGT CATTAGGAAA TGTGAATTTC ACTGTGAGCG CAGAGGCACT AGAGTCTCAA
    GAGCTGTGTG GGACTGAGGT GCCTTCAGTT CCTGAACACG GAAGGAAAGA CACAGTCATC
    AAGCCTCTGT TGGTTGAACC TGAAGGACTA GAGAAGGAAA CAACATTCAA CTCCCTACTT
    TGTCCATCAG GTGGTGAGGT TTCTGAAGAA TTATCCCTGA AACTGCCACC AAATGTGGTA
    GAAGAATCTG CCCGAGCTTC TGTCTCAGTT TTGGGAGACA TATTAGGCTC TGCCATGCAA
    AACACACAAA ATCTTCTCCA GATGCCCTAT GGCTGTGGAG AGCAGAATAT GGTCCTCTTT
    GCTCCTAACA TCTATGTACT GGATTATCTA AATGAAACAC AGCAGCTTAC TCCAGAGATC
    AAGTCCAAGG CCATTGGCTA TCTCAACACT GGTTACCAGA GACAGTTGAA CTACAAACAC
    TATGATGGCT CCTACAGCAC CTTTGGGGAG CGATATGGCA GGAACCAGGG CAACACCTGG
    CTCACAGCCT TTGTTCTGAA GACTTTTGCC CAAGCTCGAG CCTACATCTT CATCGATGAA
    GCACACATTA CCCAAGCCCT CATATGGCTC TCCCAGAGGC AGAAGGACAA TGGCTGTTTC
    AGGAGCTCTG GGTCACTGCT CAACAATGCC ATAAAGGGAG GAGTAGAAGA TGAAGTGACC
    CTCTCCGCCT ATATCACCAT CGCCCTTCTG GAGATTCCTC TCACAGTCAC TCACCCTGTT
    GTCCGCAATG CCCTGTTTTG CCTGGAGTCA GCCTGGAAGA CAGCACAAGA AGGGGACCAT
    GGCAGCCATG TATATACCAA AGCACTGCTG GCCTATGCTT TTGCCCTGGC AGGTAACCAG
    GACAAGAGGA AGGAAGTACT CAAGTCACTT AATGAGGAAG CTGTGAAGAA AGACAACTCT
    GTCCATTGGG AGCGCCCTCA GAAACCCAAG GCACCAGTGG GGCATTTTTA CGAACCCCAG
    GCTCCCTCTG CTGAGGTGGA GATGACATCC TATGTGCTCC TCGCTTATCT CACGGCCCAG
    CCAGCCCCAA CCTCGGAGGA CCTGACCTCT GCAACCAACA TCGTGAAGTG GATCACGAAG
    CAGCAGAATG CCCAGGGCGG TTTCTCCTCC ACCCAGGACA CAGTGGTGGC TCTCCATGCT
    CTGTCCAAAT ATGGAGCAGC CACATTTACC AGGACTGGGA AGGCTGCACA GGTGACTATC
    CAGTCTTCAG GGACATTTTC CAGCAAATTC CAAGTGGACA ACAACAACCG CCTGTTACTG
    CAGCAGGTCT CATTGCCAGA GCTGCCTGGG GAATACAGCA TGAAAGTGAC AGGAGAAGGA
    TGTGTCTACC TCCAGACATC CTTGAAATAC AATATTCTCC CAGAAAAGGA AGAGTTCCCC
    TTTGCTTTAG GAGTGCAGAC TCTGCCTCAA ACTTGTGATG AACCCAAAGC CCACACCAGC
    TTCCAAATCT CCCTAAGTGT CAGTTACACA GGGAGCCGCT CTGCCTCCAA CATGGCGATC
    GTTGATGTGA AGATGGTCTC TGGCTTCATT CCCCTGAAGC CAACAGTGAA AATGCTTGAA
    AGATCTAACC ATGTGAGCCG GACAGAAGTC AGCAGCAACC ATGTCTTGAT TTACCTTGAT
    AAGGTGTCAA ATCAGACACT GAGCTTGTTC TTCACGGTTC TGCAAGATGT CCCAGTAAGA
    GATCTGAAAC CAGCCATAGT GAAAGTCTAT GATTACTACG AGACGGATGA GTTTGCAATT
    GCTGAGTACA ATGCTCCTTG CAGCAAAGAT CTTGGAAATG CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102221
    Clone ID Related Accession (Same CDS sequence) NM_001347424.1
    Accession Version NM_001347424.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4125bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-27
    Organism Homo sapiens(human)
    Product alpha-2-macroglobulin isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC334781.1, DC402196.1, DB288732.1, AC007436.1, BC040071.1 and AB209614.2. Transcript Variant: This variant (3) contains an alternate splice site in the 5' region, and it thus differs in its 5' UTR and initiates translation from a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.221045.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTTCCTCA CTGTCCAAGT GAAAGGACCA ACCCAAGAAT TTAAGAAGCG GACCACAGTG 
    ATGGTTAAGA ACGAGGACAG TCTGGTCTTT GTCCAGACAG ACAAATCAAT CTACAAACCA
    GGGCAGACAG TGAAATTTCG TGTTGTCTCC ATGGATGAAA ACTTTCACCC CCTGAATGAG
    TTGATTCCAC TAGTATACAT TCAGGATCCC AAAGGAAATC GCATCGCACA ATGGCAGAGT
    TTCCAGTTAG AGGGTGGCCT CAAGCAATTT TCTTTTCCCC TCTCATCAGA GCCCTTCCAG
    GGCTCCTACA AGGTGGTGGT ACAGAAGAAA TCAGGTGGAA GGACAGAGCA CCCTTTCACC
    GTGGAGGAAT TTGTTCTTCC CAAGTTTGAA GTACAAGTAA CAGTGCCAAA GATAATCACC
    ATCTTGGAAG AAGAGATGAA TGTATCAGTG TGTGGCCTAT ACACATATGG GAAGCCTGTC
    CCTGGACATG TGACTGTGAG CATTTGCAGA AAGTATAGTG ACGCTTCCGA CTGCCACGGT
    GAAGATTCAC AGGCTTTCTG TGAGAAATTC AGTGGACAGC TAAACAGCCA TGGCTGCTTC
    TATCAGCAAG TAAAAACCAA GGTCTTCCAG CTGAAGAGGA AGGAGTATGA AATGAAACTT
    CACACTGAGG CCCAGATCCA AGAAGAAGGA ACAGTGGTGG AATTGACTGG AAGGCAGTCC
    AGTGAAATCA CAAGAACCAT AACCAAACTC TCATTTGTGA AAGTGGACTC ACACTTTCGA
    CAGGGAATTC CCTTCTTTGG GCAGGTGCGC CTAGTAGATG GGAAAGGCGT CCCTATACCA
    AATAAAGTCA TATTCATCAG AGGAAATGAA GCAAACTATT ACTCCAATGC TACCACGGAT
    GAGCATGGCC TTGTACAGTT CTCTATCAAC ACCACCAATG TTATGGGTAC CTCTCTTACT
    GTTAGGGTCA ATTACAAGGA TCGTAGTCCC TGTTACGGCT ACCAGTGGGT GTCAGAAGAA
    CACGAAGAGG CACATCACAC TGCTTATCTT GTGTTCTCCC CAAGCAAGAG CTTTGTCCAC
    CTTGAGCCCA TGTCTCATGA ACTACCCTGT GGCCATACTC AGACAGTCCA GGCACATTAT
    ATTCTGAATG GAGGCACCCT GCTGGGGCTG AAGAAGCTCT CCTTCTATTA TCTGATAATG
    GCAAAGGGAG GCATTGTCCG AACTGGGACT CATGGACTGC TTGTGAAGCA GGAAGACATG
    AAGGGCCATT TTTCCATCTC AATCCCTGTG AAGTCAGACA TTGCTCCTGT CGCTCGGTTG
    CTCATCTATG CTGTTTTACC TACCGGGGAC GTGATTGGGG ATTCTGCAAA ATATGATGTT
    GAAAATTGTC TGGCCAACAA GGTGGATTTG AGCTTCAGCC CATCACAAAG TCTCCCAGCC
    TCACACGCCC ACCTGCGAGT CACAGCGGCT CCTCAGTCCG TCTGCGCCCT CCGTGCTGTG
    GACCAAAGCG TGCTGCTCAT GAAGCCTGAT GCTGAGCTCT CGGCGTCCTC GGTTTACAAC
    CTGCTACCAG AAAAGGACCT CACTGGCTTC CCTGGGCCTT TGAATGACCA GGACGATGAA
    GACTGCATCA ATCGTCATAA TGTCTATATT AATGGAATCA CATATACTCC AGTATCAAGT
    ACAAATGAAA AGGATATGTA CAGCTTCCTA GAGGACATGG GCTTAAAGGC ATTCACCAAC
    TCAAAGATTC GTAAACCCAA AATGTGTCCA CAGCTTCAAC AGTATGAAAT GCATGGACCT
    GAAGGTCTAC GTGTAGGTTT TTATGAGTCA GATGTAATGG GAAGAGGCCA TGCACGCCTG
    GTGCATGTTG AAGAGCCTCA CACGGAGACC GTACGAAAGT ACTTCCCTGA GACATGGATC
    TGGGATTTGG TGGTGGTAAA CTCAGCAGGT GTGGCTGAGG TAGGAGTAAC AGTCCCTGAC
    ACCATCACCG AGTGGAAGGC AGGGGCCTTC TGCCTGTCTG AAGATGCTGG ACTTGGTATC
    TCTTCCACTG CCTCTCTCCG AGCCTTCCAG CCCTTCTTTG TGGAGCTCAC AATGCCTTAC
    TCTGTGATTC GTGGAGAGGC CTTCACACTC AAGGCCACGG TCCTAAACTA CCTTCCCAAA
    TGCATCCGGG TCAGTGTGCA GCTGGAAGCC TCTCCCGCCT TCCTAGCTGT CCCAGTGGAG
    AAGGAACAAG CGCCTCACTG CATCTGTGCA AACGGGCGGC AAACTGTGTC CTGGGCAGTA
    ACCCCAAAGT CATTAGGAAA TGTGAATTTC ACTGTGAGCG CAGAGGCACT AGAGTCTCAA
    GAGCTGTGTG GGACTGAGGT GCCTTCAGTT CCTGAACACG GAAGGAAAGA CACAGTCATC
    AAGCCTCTGT TGGTTGAACC TGAAGGACTA GAGAAGGAAA CAACATTCAA CTCCCTACTT
    TGTCCATCAG GTGGTGAGGT TTCTGAAGAA TTATCCCTGA AACTGCCACC AAATGTGGTA
    GAAGAATCTG CCCGAGCTTC TGTCTCAGTT TTGGGAGACA TATTAGGCTC TGCCATGCAA
    AACACACAAA ATCTTCTCCA GATGCCCTAT GGCTGTGGAG AGCAGAATAT GGTCCTCTTT
    GCTCCTAACA TCTATGTACT GGATTATCTA AATGAAACAC AGCAGCTTAC TCCAGAGATC
    AAGTCCAAGG CCATTGGCTA TCTCAACACT GGTTACCAGA GACAGTTGAA CTACAAACAC
    TATGATGGCT CCTACAGCAC CTTTGGGGAG CGATATGGCA GGAACCAGGG CAACACCTGG
    CTCACAGCCT TTGTTCTGAA GACTTTTGCC CAAGCTCGAG CCTACATCTT CATCGATGAA
    GCACACATTA CCCAAGCCCT CATATGGCTC TCCCAGAGGC AGAAGGACAA TGGCTGTTTC
    AGGAGCTCTG GGTCACTGCT CAACAATGCC ATAAAGGGAG GAGTAGAAGA TGAAGTGACC
    CTCTCCGCCT ATATCACCAT CGCCCTTCTG GAGATTCCTC TCACAGTCAC TCACCCTGTT
    GTCCGCAATG CCCTGTTTTG CCTGGAGTCA GCCTGGAAGA CAGCACAAGA AGGGGACCAT
    GGCAGCCATG TATATACCAA AGCACTGCTG GCCTATGCTT TTGCCCTGGC AGGTAACCAG
    GACAAGAGGA AGGAAGTACT CAAGTCACTT AATGAGGAAG CTGTGAAGAA AGACAACTCT
    GTCCATTGGG AGCGCCCTCA GAAACCCAAG GCACCAGTGG GGCATTTTTA CGAACCCCAG
    GCTCCCTCTG CTGAGGTGGA GATGACATCC TATGTGCTCC TCGCTTATCT CACGGCCCAG
    CCAGCCCCAA CCTCGGAGGA CCTGACCTCT GCAACCAACA TCGTGAAGTG GATCACGAAG
    CAGCAGAATG CCCAGGGCGG TTTCTCCTCC ACCCAGGACA CAGTGGTGGC TCTCCATGCT
    CTGTCCAAAT ATGGAGCAGC CACATTTACC AGGACTGGGA AGGCTGCACA GGTGACTATC
    CAGTCTTCAG GGACATTTTC CAGCAAATTC CAAGTGGACA ACAACAACCG CCTGTTACTG
    CAGCAGGTCT CATTGCCAGA GCTGCCTGGG GAATACAGCA TGAAAGTGAC AGGAGAAGGA
    TGTGTCTACC TCCAGACATC CTTGAAATAC AATATTCTCC CAGAAAAGGA AGAGTTCCCC
    TTTGCTTTAG GAGTGCAGAC TCTGCCTCAA ACTTGTGATG AACCCAAAGC CCACACCAGC
    TTCCAAATCT CCCTAAGTGT CAGTTACACA GGGAGCCGCT CTGCCTCCAA CATGGCGATC
    GTTGATGTGA AGATGGTCTC TGGCTTCATT CCCCTGAAGC CAACAGTGAA AATGCTTGAA
    AGATCTAACC ATGTGAGCCG GACAGAAGTC AGCAGCAACC ATGTCTTGAT TTACCTTGAT
    AAGGTGTCAA ATCAGACACT GAGCTTGTTC TTCACGGTTC TGCAAGATGT CCCAGTAAGA
    GATCTGAAAC CAGCCATAGT GAAAGTCTAT GATTACTACG AGACGGATGA GTTTGCAATT
    GCTGAGTACA ATGCTCCTTG CAGCAAAGAT CTTGGAAATG CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001334353.1
    CDS834..4958
    Translation

    Target ORF information:

    RefSeq Version NM_001347424.1
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha-2-macroglobulin (A2M), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001347424.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTTCCTCA CTGTCCAAGT GAAAGGACCA ACCCAAGAAT TTAAGAAGCG GACCACAGTG 
    ATGGTTAAGA ACGAGGACAG TCTGGTCTTT GTCCAGACAG ACAAATCAAT CTACAAACCA
    GGGCAGACAG TGAAATTTCG TGTTGTCTCC ATGGATGAAA ACTTTCACCC CCTGAATGAG
    TTGATTCCAC TAGTATACAT TCAGGATCCC AAAGGAAATC GCATCGCACA ATGGCAGAGT
    TTCCAGTTAG AGGGTGGCCT CAAGCAATTT TCTTTTCCCC TCTCATCAGA GCCCTTCCAG
    GGCTCCTACA AGGTGGTGGT ACAGAAGAAA TCAGGTGGAA GGACAGAGCA CCCTTTCACC
    GTGGAGGAAT TTGTTCTTCC CAAGTTTGAA GTACAAGTAA CAGTGCCAAA GATAATCACC
    ATCTTGGAAG AAGAGATGAA TGTATCAGTG TGTGGCCTAT ACACATATGG GAAGCCTGTC
    CCTGGACATG TGACTGTGAG CATTTGCAGA AAGTATAGTG ACGCTTCCGA CTGCCACGGT
    GAAGATTCAC AGGCTTTCTG TGAGAAATTC AGTGGACAGC TAAACAGCCA TGGCTGCTTC
    TATCAGCAAG TAAAAACCAA GGTCTTCCAG CTGAAGAGGA AGGAGTATGA AATGAAACTT
    CACACTGAGG CCCAGATCCA AGAAGAAGGA ACAGTGGTGG AATTGACTGG AAGGCAGTCC
    AGTGAAATCA CAAGAACCAT AACCAAACTC TCATTTGTGA AAGTGGACTC ACACTTTCGA
    CAGGGAATTC CCTTCTTTGG GCAGGTGCGC CTAGTAGATG GGAAAGGCGT CCCTATACCA
    AATAAAGTCA TATTCATCAG AGGAAATGAA GCAAACTATT ACTCCAATGC TACCACGGAT
    GAGCATGGCC TTGTACAGTT CTCTATCAAC ACCACCAATG TTATGGGTAC CTCTCTTACT
    GTTAGGGTCA ATTACAAGGA TCGTAGTCCC TGTTACGGCT ACCAGTGGGT GTCAGAAGAA
    CACGAAGAGG CACATCACAC TGCTTATCTT GTGTTCTCCC CAAGCAAGAG CTTTGTCCAC
    CTTGAGCCCA TGTCTCATGA ACTACCCTGT GGCCATACTC AGACAGTCCA GGCACATTAT
    ATTCTGAATG GAGGCACCCT GCTGGGGCTG AAGAAGCTCT CCTTCTATTA TCTGATAATG
    GCAAAGGGAG GCATTGTCCG AACTGGGACT CATGGACTGC TTGTGAAGCA GGAAGACATG
    AAGGGCCATT TTTCCATCTC AATCCCTGTG AAGTCAGACA TTGCTCCTGT CGCTCGGTTG
    CTCATCTATG CTGTTTTACC TACCGGGGAC GTGATTGGGG ATTCTGCAAA ATATGATGTT
    GAAAATTGTC TGGCCAACAA GGTGGATTTG AGCTTCAGCC CATCACAAAG TCTCCCAGCC
    TCACACGCCC ACCTGCGAGT CACAGCGGCT CCTCAGTCCG TCTGCGCCCT CCGTGCTGTG
    GACCAAAGCG TGCTGCTCAT GAAGCCTGAT GCTGAGCTCT CGGCGTCCTC GGTTTACAAC
    CTGCTACCAG AAAAGGACCT CACTGGCTTC CCTGGGCCTT TGAATGACCA GGACGATGAA
    GACTGCATCA ATCGTCATAA TGTCTATATT AATGGAATCA CATATACTCC AGTATCAAGT
    ACAAATGAAA AGGATATGTA CAGCTTCCTA GAGGACATGG GCTTAAAGGC ATTCACCAAC
    TCAAAGATTC GTAAACCCAA AATGTGTCCA CAGCTTCAAC AGTATGAAAT GCATGGACCT
    GAAGGTCTAC GTGTAGGTTT TTATGAGTCA GATGTAATGG GAAGAGGCCA TGCACGCCTG
    GTGCATGTTG AAGAGCCTCA CACGGAGACC GTACGAAAGT ACTTCCCTGA GACATGGATC
    TGGGATTTGG TGGTGGTAAA CTCAGCAGGT GTGGCTGAGG TAGGAGTAAC AGTCCCTGAC
    ACCATCACCG AGTGGAAGGC AGGGGCCTTC TGCCTGTCTG AAGATGCTGG ACTTGGTATC
    TCTTCCACTG CCTCTCTCCG AGCCTTCCAG CCCTTCTTTG TGGAGCTCAC AATGCCTTAC
    TCTGTGATTC GTGGAGAGGC CTTCACACTC AAGGCCACGG TCCTAAACTA CCTTCCCAAA
    TGCATCCGGG TCAGTGTGCA GCTGGAAGCC TCTCCCGCCT TCCTAGCTGT CCCAGTGGAG
    AAGGAACAAG CGCCTCACTG CATCTGTGCA AACGGGCGGC AAACTGTGTC CTGGGCAGTA
    ACCCCAAAGT CATTAGGAAA TGTGAATTTC ACTGTGAGCG CAGAGGCACT AGAGTCTCAA
    GAGCTGTGTG GGACTGAGGT GCCTTCAGTT CCTGAACACG GAAGGAAAGA CACAGTCATC
    AAGCCTCTGT TGGTTGAACC TGAAGGACTA GAGAAGGAAA CAACATTCAA CTCCCTACTT
    TGTCCATCAG GTGGTGAGGT TTCTGAAGAA TTATCCCTGA AACTGCCACC AAATGTGGTA
    GAAGAATCTG CCCGAGCTTC TGTCTCAGTT TTGGGAGACA TATTAGGCTC TGCCATGCAA
    AACACACAAA ATCTTCTCCA GATGCCCTAT GGCTGTGGAG AGCAGAATAT GGTCCTCTTT
    GCTCCTAACA TCTATGTACT GGATTATCTA AATGAAACAC AGCAGCTTAC TCCAGAGATC
    AAGTCCAAGG CCATTGGCTA TCTCAACACT GGTTACCAGA GACAGTTGAA CTACAAACAC
    TATGATGGCT CCTACAGCAC CTTTGGGGAG CGATATGGCA GGAACCAGGG CAACACCTGG
    CTCACAGCCT TTGTTCTGAA GACTTTTGCC CAAGCTCGAG CCTACATCTT CATCGATGAA
    GCACACATTA CCCAAGCCCT CATATGGCTC TCCCAGAGGC AGAAGGACAA TGGCTGTTTC
    AGGAGCTCTG GGTCACTGCT CAACAATGCC ATAAAGGGAG GAGTAGAAGA TGAAGTGACC
    CTCTCCGCCT ATATCACCAT CGCCCTTCTG GAGATTCCTC TCACAGTCAC TCACCCTGTT
    GTCCGCAATG CCCTGTTTTG CCTGGAGTCA GCCTGGAAGA CAGCACAAGA AGGGGACCAT
    GGCAGCCATG TATATACCAA AGCACTGCTG GCCTATGCTT TTGCCCTGGC AGGTAACCAG
    GACAAGAGGA AGGAAGTACT CAAGTCACTT AATGAGGAAG CTGTGAAGAA AGACAACTCT
    GTCCATTGGG AGCGCCCTCA GAAACCCAAG GCACCAGTGG GGCATTTTTA CGAACCCCAG
    GCTCCCTCTG CTGAGGTGGA GATGACATCC TATGTGCTCC TCGCTTATCT CACGGCCCAG
    CCAGCCCCAA CCTCGGAGGA CCTGACCTCT GCAACCAACA TCGTGAAGTG GATCACGAAG
    CAGCAGAATG CCCAGGGCGG TTTCTCCTCC ACCCAGGACA CAGTGGTGGC TCTCCATGCT
    CTGTCCAAAT ATGGAGCAGC CACATTTACC AGGACTGGGA AGGCTGCACA GGTGACTATC
    CAGTCTTCAG GGACATTTTC CAGCAAATTC CAAGTGGACA ACAACAACCG CCTGTTACTG
    CAGCAGGTCT CATTGCCAGA GCTGCCTGGG GAATACAGCA TGAAAGTGAC AGGAGAAGGA
    TGTGTCTACC TCCAGACATC CTTGAAATAC AATATTCTCC CAGAAAAGGA AGAGTTCCCC
    TTTGCTTTAG GAGTGCAGAC TCTGCCTCAA ACTTGTGATG AACCCAAAGC CCACACCAGC
    TTCCAAATCT CCCTAAGTGT CAGTTACACA GGGAGCCGCT CTGCCTCCAA CATGGCGATC
    GTTGATGTGA AGATGGTCTC TGGCTTCATT CCCCTGAAGC CAACAGTGAA AATGCTTGAA
    AGATCTAACC ATGTGAGCCG GACAGAAGTC AGCAGCAACC ATGTCTTGAT TTACCTTGAT
    AAGGTGTCAA ATCAGACACT GAGCTTGTTC TTCACGGTTC TGCAAGATGT CCCAGTAAGA
    GATCTGAAAC CAGCCATAGT GAAAGTCTAT GATTACTACG AGACGGATGA GTTTGCAATT
    GCTGAGTACA ATGCTCCTTG CAGCAAAGAT CTTGGAAATG CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102390
    Clone ID Related Accession (Same CDS sequence) NM_001347425.1
    Accession Version NM_001347425.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-27
    Organism Homo sapiens(human)
    Product alpha-2-macroglobulin isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC334781.1, DC402196.1, AK307832.1 and BC040071.1. Transcript Variant: This variant (4) lacks an exon in the 5' region, and it thus differs in its 5' UTR and initiates translation from a downstream in-frame start codon, compared to variant 1. The encoded isoform (c) is shorter at the N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.96034.1, AK307832.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGAAA ACTTTCACCC CCTGAATGAG TTGATTCCAC TAGTATACAT TCAGGATCCC 
    AAAGGAAATC GCATCGCACA ATGGCAGAGT TTCCAGTTAG AGGGTGGCCT CAAGCAATTT
    TCTTTTCCCC TCTCATCAGA GCCCTTCCAG GGCTCCTACA AGGTGGTGGT ACAGAAGAAA
    TCAGGTGGAA GGACAGAGCA CCCTTTCACC GTGGAGGAAT TTGTTCTTCC CAAGTTTGAA
    GTACAAGTAA CAGTGCCAAA GATAATCACC ATCTTGGAAG AAGAGATGAA TGTATCAGTG
    TGTGGCCTAT ACACATATGG GAAGCCTGTC CCTGGACATG TGACTGTGAG CATTTGCAGA
    AAGTATAGTG ACGCTTCCGA CTGCCACGGT GAAGATTCAC AGGCTTTCTG TGAGAAATTC
    AGTGGACAGC TAAACAGCCA TGGCTGCTTC TATCAGCAAG TAAAAACCAA GGTCTTCCAG
    CTGAAGAGGA AGGAGTATGA AATGAAACTT CACACTGAGG CCCAGATCCA AGAAGAAGGA
    ACAGTGGTGG AATTGACTGG AAGGCAGTCC AGTGAAATCA CAAGAACCAT AACCAAACTC
    TCATTTGTGA AAGTGGACTC ACACTTTCGA CAGGGAATTC CCTTCTTTGG GCAGGTGCGC
    CTAGTAGATG GGAAAGGCGT CCCTATACCA AATAAAGTCA TATTCATCAG AGGAAATGAA
    GCAAACTATT ACTCCAATGC TACCACGGAT GAGCATGGCC TTGTACAGTT CTCTATCAAC
    ACCACCAATG TTATGGGTAC CTCTCTTACT GTTAGGGTCA ATTACAAGGA TCGTAGTCCC
    TGTTACGGCT ACCAGTGGGT GTCAGAAGAA CACGAAGAGG CACATCACAC TGCTTATCTT
    GTGTTCTCCC CAAGCAAGAG CTTTGTCCAC CTTGAGCCCA TGTCTCATGA ACTACCCTGT
    GGCCATACTC AGACAGTCCA GGCACATTAT ATTCTGAATG GAGGCACCCT GCTGGGGCTG
    AAGAAGCTCT CCTTCTATTA TCTGATAATG GCAAAGGGAG GCATTGTCCG AACTGGGACT
    CATGGACTGC TTGTGAAGCA GGAAGACATG AAGGGCCATT TTTCCATCTC AATCCCTGTG
    AAGTCAGACA TTGCTCCTGT CGCTCGGTTG CTCATCTATG CTGTTTTACC TACCGGGGAC
    GTGATTGGGG ATTCTGCAAA ATATGATGTT GAAAATTGTC TGGCCAACAA GGTGGATTTG
    AGCTTCAGCC CATCACAAAG TCTCCCAGCC TCACACGCCC ACCTGCGAGT CACAGCGGCT
    CCTCAGTCCG TCTGCGCCCT CCGTGCTGTG GACCAAAGCG TGCTGCTCAT GAAGCCTGAT
    GCTGAGCTCT CGGCGTCCTC GGTTTACAAC CTGCTACCAG AAAAGGACCT CACTGGCTTC
    CCTGGGCCTT TGAATGACCA GGACGATGAA GACTGCATCA ATCGTCATAA TGTCTATATT
    AATGGAATCA CATATACTCC AGTATCAAGT ACAAATGAAA AGGATATGTA CAGCTTCCTA
    GAGGACATGG GCTTAAAGGC ATTCACCAAC TCAAAGATTC GTAAACCCAA AATGTGTCCA
    CAGCTTCAAC AGTATGAAAT GCATGGACCT GAAGGTCTAC GTGTAGGTTT TTATGAGTCA
    GATGTAATGG GAAGAGGCCA TGCACGCCTG GTGCATGTTG AAGAGCCTCA CACGGAGACC
    GTACGAAAGT ACTTCCCTGA GACATGGATC TGGGATTTGG TGGTGGTAAA CTCAGCAGGT
    GTGGCTGAGG TAGGAGTAAC AGTCCCTGAC ACCATCACCG AGTGGAAGGC AGGGGCCTTC
    TGCCTGTCTG AAGATGCTGG ACTTGGTATC TCTTCCACTG CCTCTCTCCG AGCCTTCCAG
    CCCTTCTTTG TGGAGCTCAC AATGCCTTAC TCTGTGATTC GTGGAGAGGC CTTCACACTC
    AAGGCCACGG TCCTAAACTA CCTTCCCAAA TGCATCCGGG TCAGTGTGCA GCTGGAAGCC
    TCTCCCGCCT TCCTAGCTGT CCCAGTGGAG AAGGAACAAG CGCCTCACTG CATCTGTGCA
    AACGGGCGGC AAACTGTGTC CTGGGCAGTA ACCCCAAAGT CATTAGGAAA TGTGAATTTC
    ACTGTGAGCG CAGAGGCACT AGAGTCTCAA GAGCTGTGTG GGACTGAGGT GCCTTCAGTT
    CCTGAACACG GAAGGAAAGA CACAGTCATC AAGCCTCTGT TGGTTGAACC TGAAGGACTA
    GAGAAGGAAA CAACATTCAA CTCCCTACTT TGTCCATCAG GTGGTGAGGT TTCTGAAGAA
    TTATCCCTGA AACTGCCACC AAATGTGGTA GAAGAATCTG CCCGAGCTTC TGTCTCAGTT
    TTGGGAGACA TATTAGGCTC TGCCATGCAA AACACACAAA ATCTTCTCCA GATGCCCTAT
    GGCTGTGGAG AGCAGAATAT GGTCCTCTTT GCTCCTAACA TCTATGTACT GGATTATCTA
    AATGAAACAC AGCAGCTTAC TCCAGAGATC AAGTCCAAGG CCATTGGCTA TCTCAACACT
    GGTTACCAGA GACAGTTGAA CTACAAACAC TATGATGGCT CCTACAGCAC CTTTGGGGAG
    CGATATGGCA GGAACCAGGG CAACACCTGG CTCACAGCCT TTGTTCTGAA GACTTTTGCC
    CAAGCTCGAG CCTACATCTT CATCGATGAA GCACACATTA CCCAAGCCCT CATATGGCTC
    TCCCAGAGGC AGAAGGACAA TGGCTGTTTC AGGAGCTCTG GGTCACTGCT CAACAATGCC
    ATAAAGGGAG GAGTAGAAGA TGAAGTGACC CTCTCCGCCT ATATCACCAT CGCCCTTCTG
    GAGATTCCTC TCACAGTCAC TCACCCTGTT GTCCGCAATG CCCTGTTTTG CCTGGAGTCA
    GCCTGGAAGA CAGCACAAGA AGGGGACCAT GGCAGCCATG TATATACCAA AGCACTGCTG
    GCCTATGCTT TTGCCCTGGC AGGTAACCAG GACAAGAGGA AGGAAGTACT CAAGTCACTT
    AATGAGGAAG CTGTGAAGAA AGACAACTCT GTCCATTGGG AGCGCCCTCA GAAACCCAAG
    GCACCAGTGG GGCATTTTTA CGAACCCCAG GCTCCCTCTG CTGAGGTGGA GATGACATCC
    TATGTGCTCC TCGCTTATCT CACGGCCCAG CCAGCCCCAA CCTCGGAGGA CCTGACCTCT
    GCAACCAACA TCGTGAAGTG GATCACGAAG CAGCAGAATG CCCAGGGCGG TTTCTCCTCC
    ACCCAGGACA CAGTGGTGGC TCTCCATGCT CTGTCCAAAT ATGGAGCAGC CACATTTACC
    AGGACTGGGA AGGCTGCACA GGTGACTATC CAGTCTTCAG GGACATTTTC CAGCAAATTC
    CAAGTGGACA ACAACAACCG CCTGTTACTG CAGCAGGTCT CATTGCCAGA GCTGCCTGGG
    GAATACAGCA TGAAAGTGAC AGGAGAAGGA TGTGTCTACC TCCAGACATC CTTGAAATAC
    AATATTCTCC CAGAAAAGGA AGAGTTCCCC TTTGCTTTAG GAGTGCAGAC TCTGCCTCAA
    ACTTGTGATG AACCCAAAGC CCACACCAGC TTCCAAATCT CCCTAAGTGT CAGTTACACA
    GGGAGCCGCT CTGCCTCCAA CATGGCGATC GTTGATGTGA AGATGGTCTC TGGCTTCATT
    CCCCTGAAGC CAACAGTGAA AATGCTTGAA AGATCTAACC ATGTGAGCCG GACAGAAGTC
    AGCAGCAACC ATGTCTTGAT TTACCTTGAT AAGGTGTCAA ATCAGACACT GAGCTTGTTC
    TTCACGGTTC TGCAAGATGT CCCAGTAAGA GATCTGAAAC CAGCCATAGT GAAAGTCTAT
    GATTACTACG AGACGGATGA GTTTGCAATT GCTGAGTACA ATGCTCCTTG CAGCAAAGAT
    CTTGGAAATG CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001334354.1
    CDS671..4645
    Translation

    Target ORF information:

    RefSeq Version NM_001347425.1
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha-2-macroglobulin (A2M), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001347425.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGAAA ACTTTCACCC CCTGAATGAG TTGATTCCAC TAGTATACAT TCAGGATCCC 
    AAAGGAAATC GCATCGCACA ATGGCAGAGT TTCCAGTTAG AGGGTGGCCT CAAGCAATTT
    TCTTTTCCCC TCTCATCAGA GCCCTTCCAG GGCTCCTACA AGGTGGTGGT ACAGAAGAAA
    TCAGGTGGAA GGACAGAGCA CCCTTTCACC GTGGAGGAAT TTGTTCTTCC CAAGTTTGAA
    GTACAAGTAA CAGTGCCAAA GATAATCACC ATCTTGGAAG AAGAGATGAA TGTATCAGTG
    TGTGGCCTAT ACACATATGG GAAGCCTGTC CCTGGACATG TGACTGTGAG CATTTGCAGA
    AAGTATAGTG ACGCTTCCGA CTGCCACGGT GAAGATTCAC AGGCTTTCTG TGAGAAATTC
    AGTGGACAGC TAAACAGCCA TGGCTGCTTC TATCAGCAAG TAAAAACCAA GGTCTTCCAG
    CTGAAGAGGA AGGAGTATGA AATGAAACTT CACACTGAGG CCCAGATCCA AGAAGAAGGA
    ACAGTGGTGG AATTGACTGG AAGGCAGTCC AGTGAAATCA CAAGAACCAT AACCAAACTC
    TCATTTGTGA AAGTGGACTC ACACTTTCGA CAGGGAATTC CCTTCTTTGG GCAGGTGCGC
    CTAGTAGATG GGAAAGGCGT CCCTATACCA AATAAAGTCA TATTCATCAG AGGAAATGAA
    GCAAACTATT ACTCCAATGC TACCACGGAT GAGCATGGCC TTGTACAGTT CTCTATCAAC
    ACCACCAATG TTATGGGTAC CTCTCTTACT GTTAGGGTCA ATTACAAGGA TCGTAGTCCC
    TGTTACGGCT ACCAGTGGGT GTCAGAAGAA CACGAAGAGG CACATCACAC TGCTTATCTT
    GTGTTCTCCC CAAGCAAGAG CTTTGTCCAC CTTGAGCCCA TGTCTCATGA ACTACCCTGT
    GGCCATACTC AGACAGTCCA GGCACATTAT ATTCTGAATG GAGGCACCCT GCTGGGGCTG
    AAGAAGCTCT CCTTCTATTA TCTGATAATG GCAAAGGGAG GCATTGTCCG AACTGGGACT
    CATGGACTGC TTGTGAAGCA GGAAGACATG AAGGGCCATT TTTCCATCTC AATCCCTGTG
    AAGTCAGACA TTGCTCCTGT CGCTCGGTTG CTCATCTATG CTGTTTTACC TACCGGGGAC
    GTGATTGGGG ATTCTGCAAA ATATGATGTT GAAAATTGTC TGGCCAACAA GGTGGATTTG
    AGCTTCAGCC CATCACAAAG TCTCCCAGCC TCACACGCCC ACCTGCGAGT CACAGCGGCT
    CCTCAGTCCG TCTGCGCCCT CCGTGCTGTG GACCAAAGCG TGCTGCTCAT GAAGCCTGAT
    GCTGAGCTCT CGGCGTCCTC GGTTTACAAC CTGCTACCAG AAAAGGACCT CACTGGCTTC
    CCTGGGCCTT TGAATGACCA GGACGATGAA GACTGCATCA ATCGTCATAA TGTCTATATT
    AATGGAATCA CATATACTCC AGTATCAAGT ACAAATGAAA AGGATATGTA CAGCTTCCTA
    GAGGACATGG GCTTAAAGGC ATTCACCAAC TCAAAGATTC GTAAACCCAA AATGTGTCCA
    CAGCTTCAAC AGTATGAAAT GCATGGACCT GAAGGTCTAC GTGTAGGTTT TTATGAGTCA
    GATGTAATGG GAAGAGGCCA TGCACGCCTG GTGCATGTTG AAGAGCCTCA CACGGAGACC
    GTACGAAAGT ACTTCCCTGA GACATGGATC TGGGATTTGG TGGTGGTAAA CTCAGCAGGT
    GTGGCTGAGG TAGGAGTAAC AGTCCCTGAC ACCATCACCG AGTGGAAGGC AGGGGCCTTC
    TGCCTGTCTG AAGATGCTGG ACTTGGTATC TCTTCCACTG CCTCTCTCCG AGCCTTCCAG
    CCCTTCTTTG TGGAGCTCAC AATGCCTTAC TCTGTGATTC GTGGAGAGGC CTTCACACTC
    AAGGCCACGG TCCTAAACTA CCTTCCCAAA TGCATCCGGG TCAGTGTGCA GCTGGAAGCC
    TCTCCCGCCT TCCTAGCTGT CCCAGTGGAG AAGGAACAAG CGCCTCACTG CATCTGTGCA
    AACGGGCGGC AAACTGTGTC CTGGGCAGTA ACCCCAAAGT CATTAGGAAA TGTGAATTTC
    ACTGTGAGCG CAGAGGCACT AGAGTCTCAA GAGCTGTGTG GGACTGAGGT GCCTTCAGTT
    CCTGAACACG GAAGGAAAGA CACAGTCATC AAGCCTCTGT TGGTTGAACC TGAAGGACTA
    GAGAAGGAAA CAACATTCAA CTCCCTACTT TGTCCATCAG GTGGTGAGGT TTCTGAAGAA
    TTATCCCTGA AACTGCCACC AAATGTGGTA GAAGAATCTG CCCGAGCTTC TGTCTCAGTT
    TTGGGAGACA TATTAGGCTC TGCCATGCAA AACACACAAA ATCTTCTCCA GATGCCCTAT
    GGCTGTGGAG AGCAGAATAT GGTCCTCTTT GCTCCTAACA TCTATGTACT GGATTATCTA
    AATGAAACAC AGCAGCTTAC TCCAGAGATC AAGTCCAAGG CCATTGGCTA TCTCAACACT
    GGTTACCAGA GACAGTTGAA CTACAAACAC TATGATGGCT CCTACAGCAC CTTTGGGGAG
    CGATATGGCA GGAACCAGGG CAACACCTGG CTCACAGCCT TTGTTCTGAA GACTTTTGCC
    CAAGCTCGAG CCTACATCTT CATCGATGAA GCACACATTA CCCAAGCCCT CATATGGCTC
    TCCCAGAGGC AGAAGGACAA TGGCTGTTTC AGGAGCTCTG GGTCACTGCT CAACAATGCC
    ATAAAGGGAG GAGTAGAAGA TGAAGTGACC CTCTCCGCCT ATATCACCAT CGCCCTTCTG
    GAGATTCCTC TCACAGTCAC TCACCCTGTT GTCCGCAATG CCCTGTTTTG CCTGGAGTCA
    GCCTGGAAGA CAGCACAAGA AGGGGACCAT GGCAGCCATG TATATACCAA AGCACTGCTG
    GCCTATGCTT TTGCCCTGGC AGGTAACCAG GACAAGAGGA AGGAAGTACT CAAGTCACTT
    AATGAGGAAG CTGTGAAGAA AGACAACTCT GTCCATTGGG AGCGCCCTCA GAAACCCAAG
    GCACCAGTGG GGCATTTTTA CGAACCCCAG GCTCCCTCTG CTGAGGTGGA GATGACATCC
    TATGTGCTCC TCGCTTATCT CACGGCCCAG CCAGCCCCAA CCTCGGAGGA CCTGACCTCT
    GCAACCAACA TCGTGAAGTG GATCACGAAG CAGCAGAATG CCCAGGGCGG TTTCTCCTCC
    ACCCAGGACA CAGTGGTGGC TCTCCATGCT CTGTCCAAAT ATGGAGCAGC CACATTTACC
    AGGACTGGGA AGGCTGCACA GGTGACTATC CAGTCTTCAG GGACATTTTC CAGCAAATTC
    CAAGTGGACA ACAACAACCG CCTGTTACTG CAGCAGGTCT CATTGCCAGA GCTGCCTGGG
    GAATACAGCA TGAAAGTGAC AGGAGAAGGA TGTGTCTACC TCCAGACATC CTTGAAATAC
    AATATTCTCC CAGAAAAGGA AGAGTTCCCC TTTGCTTTAG GAGTGCAGAC TCTGCCTCAA
    ACTTGTGATG AACCCAAAGC CCACACCAGC TTCCAAATCT CCCTAAGTGT CAGTTACACA
    GGGAGCCGCT CTGCCTCCAA CATGGCGATC GTTGATGTGA AGATGGTCTC TGGCTTCATT
    CCCCTGAAGC CAACAGTGAA AATGCTTGAA AGATCTAACC ATGTGAGCCG GACAGAAGTC
    AGCAGCAACC ATGTCTTGAT TTACCTTGAT AAGGTGTCAA ATCAGACACT GAGCTTGTTC
    TTCACGGTTC TGCAAGATGT CCCAGTAAGA GATCTGAAAC CAGCCATAGT GAAAGTCTAT
    GATTACTACG AGACGGATGA GTTTGCAATT GCTGAGTACA ATGCTCCTTG CAGCAAAGAT
    CTTGGAAATG CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108400
    Clone ID Related Accession (Same CDS sequence) NM_001347423.2 , NM_000014.6
    Accession Version NM_001347423.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4425bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-02
    Organism Homo sapiens(human)
    Product alpha-2-macroglobulin isoform a precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC007436.1. On Jun 2, 2019 this sequence version replaced NM_001347423.1. Transcript Variant: This variant (2) splices out a region of the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.140912.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGGGAAGA ACAAACTCCT TCATCCAAGT CTGGTTCTTC TCCTCTTGGT CCTCCTGCCC 
    ACAGACGCCT CAGTCTCTGG AAAACCGCAG TATATGGTTC TGGTCCCCTC CCTGCTCCAC
    ACTGAGACCA CTGAGAAGGG CTGTGTCCTT CTGAGCTACC TGAATGAGAC AGTGACTGTA
    AGTGCTTCCT TGGAGTCTGT CAGGGGAAAC AGGAGCCTCT TCACTGACCT GGAGGCGGAG
    AATGACGTAC TCCACTGTGT CGCCTTCGCT GTCCCAAAGT CTTCATCCAA TGAGGAGGTA
    ATGTTCCTCA CTGTCCAAGT GAAAGGACCA ACCCAAGAAT TTAAGAAGCG GACCACAGTG
    ATGGTTAAGA ACGAGGACAG TCTGGTCTTT GTCCAGACAG ACAAATCAAT CTACAAACCA
    GGGCAGACAG TGAAATTTCG TGTTGTCTCC ATGGATGAAA ACTTTCACCC CCTGAATGAG
    TTGATTCCAC TAGTATACAT TCAGGATCCC AAAGGAAATC GCATCGCACA ATGGCAGAGT
    TTCCAGTTAG AGGGTGGCCT CAAGCAATTT TCTTTTCCCC TCTCATCAGA GCCCTTCCAG
    GGCTCCTACA AGGTGGTGGT ACAGAAGAAA TCAGGTGGAA GGACAGAGCA CCCTTTCACC
    GTGGAGGAAT TTGTTCTTCC CAAGTTTGAA GTACAAGTAA CAGTGCCAAA GATAATCACC
    ATCTTGGAAG AAGAGATGAA TGTATCAGTG TGTGGCCTAT ACACATATGG GAAGCCTGTC
    CCTGGACATG TGACTGTGAG CATTTGCAGA AAGTATAGTG ACGCTTCCGA CTGCCACGGT
    GAAGATTCAC AGGCTTTCTG TGAGAAATTC AGTGGACAGC TAAACAGCCA TGGCTGCTTC
    TATCAGCAAG TAAAAACCAA GGTCTTCCAG CTGAAGAGGA AGGAGTATGA AATGAAACTT
    CACACTGAGG CCCAGATCCA AGAAGAAGGA ACAGTGGTGG AATTGACTGG AAGGCAGTCC
    AGTGAAATCA CAAGAACCAT AACCAAACTC TCATTTGTGA AAGTGGACTC ACACTTTCGA
    CAGGGAATTC CCTTCTTTGG GCAGGTGCGC CTAGTAGATG GGAAAGGCGT CCCTATACCA
    AATAAAGTCA TATTCATCAG AGGAAATGAA GCAAACTATT ACTCCAATGC TACCACGGAT
    GAGCATGGCC TTGTACAGTT CTCTATCAAC ACCACCAATG TTATGGGTAC CTCTCTTACT
    GTTAGGGTCA ATTACAAGGA TCGTAGTCCC TGTTACGGCT ACCAGTGGGT GTCAGAAGAA
    CACGAAGAGG CACATCACAC TGCTTATCTT GTGTTCTCCC CAAGCAAGAG CTTTGTCCAC
    CTTGAGCCCA TGTCTCATGA ACTACCCTGT GGCCATACTC AGACAGTCCA GGCACATTAT
    ATTCTGAATG GAGGCACCCT GCTGGGGCTG AAGAAGCTCT CCTTCTATTA TCTGATAATG
    GCAAAGGGAG GCATTGTCCG AACTGGGACT CATGGACTGC TTGTGAAGCA GGAAGACATG
    AAGGGCCATT TTTCCATCTC AATCCCTGTG AAGTCAGACA TTGCTCCTGT CGCTCGGTTG
    CTCATCTATG CTGTTTTACC TACCGGGGAC GTGATTGGGG ATTCTGCAAA ATATGATGTT
    GAAAATTGTC TGGCCAACAA GGTGGATTTG AGCTTCAGCC CATCACAAAG TCTCCCAGCC
    TCACACGCCC ACCTGCGAGT CACAGCGGCT CCTCAGTCCG TCTGCGCCCT CCGTGCTGTG
    GACCAAAGCG TGCTGCTCAT GAAGCCTGAT GCTGAGCTCT CGGCGTCCTC GGTTTACAAC
    CTGCTACCAG AAAAGGACCT CACTGGCTTC CCTGGGCCTT TGAATGACCA GGACAATGAA
    GACTGCATCA ATCGTCATAA TGTCTATATT AATGGAATCA CATATACTCC AGTATCAAGT
    ACAAATGAAA AGGATATGTA CAGCTTCCTA GAGGACATGG GCTTAAAGGC ATTCACCAAC
    TCAAAGATTC GTAAACCCAA AATGTGTCCA CAGCTTCAAC AGTATGAAAT GCATGGACCT
    GAAGGTCTAC GTGTAGGTTT TTATGAGTCA GATGTAATGG GAAGAGGCCA TGCACGCCTG
    GTGCATGTTG AAGAGCCTCA CACGGAGACC GTACGAAAGT ACTTCCCTGA GACATGGATC
    TGGGATTTGG TGGTGGTAAA CTCAGCAGGT GTGGCTGAGG TAGGAGTAAC AGTCCCTGAC
    ACCATCACCG AGTGGAAGGC AGGGGCCTTC TGCCTGTCTG AAGATGCTGG ACTTGGTATC
    TCTTCCACTG CCTCTCTCCG AGCCTTCCAG CCCTTCTTTG TGGAGCTCAC AATGCCTTAC
    TCTGTGATTC GTGGAGAGGC CTTCACACTC AAGGCCACGG TCCTAAACTA CCTTCCCAAA
    TGCATCCGGG TCAGTGTGCA GCTGGAAGCC TCTCCCGCCT TCCTAGCTGT CCCAGTGGAG
    AAGGAACAAG CGCCTCACTG CATCTGTGCA AACGGGCGGC AAACTGTGTC CTGGGCAGTA
    ACCCCAAAGT CATTAGGAAA TGTGAATTTC ACTGTGAGCG CAGAGGCACT AGAGTCTCAA
    GAGCTGTGTG GGACTGAGGT GCCTTCAGTT CCTGAACACG GAAGGAAAGA CACAGTCATC
    AAGCCTCTGT TGGTTGAACC TGAAGGACTA GAGAAGGAAA CAACATTCAA CTCCCTACTT
    TGTCCATCAG GTGGTGAGGT TTCTGAAGAA TTATCCCTGA AACTGCCACC AAATGTGGTA
    GAAGAATCTG CCCGAGCTTC TGTCTCAGTT TTGGGAGACA TATTAGGCTC TGCCATGCAA
    AACACACAAA ATCTTCTCCA GATGCCCTAT GGCTGTGGAG AGCAGAATAT GGTCCTCTTT
    GCTCCTAACA TCTATGTACT GGATTATCTA AATGAAACAC AGCAGCTTAC TCCAGAGATC
    AAGTCCAAGG CCATTGGCTA TCTCAACACT GGTTACCAGA GACAGTTGAA CTACAAACAC
    TATGATGGCT CCTACAGCAC CTTTGGGGAG CGATATGGCA GGAACCAGGG CAACACCTGG
    CTCACAGCCT TTGTTCTGAA GACTTTTGCC CAAGCTCGAG CCTACATCTT CATCGATGAA
    GCACACATTA CCCAAGCCCT CATATGGCTC TCCCAGAGGC AGAAGGACAA TGGCTGTTTC
    AGGAGCTCTG GGTCACTGCT CAACAATGCC ATAAAGGGAG GAGTAGAAGA TGAAGTGACC
    CTCTCCGCCT ATATCACCAT CGCCCTTCTG GAGATTCCTC TCACAGTCAC TCACCCTGTT
    GTCCGCAATG CCCTGTTTTG CCTGGAGTCA GCCTGGAAGA CAGCACAAGA AGGGGACCAT
    GGCAGCCATG TATATACCAA AGCACTGCTG GCCTATGCTT TTGCCCTGGC AGGTAACCAG
    GACAAGAGGA AGGAAGTACT CAAGTCACTT AATGAGGAAG CTGTGAAGAA AGACAACTCT
    GTCCATTGGG AGCGCCCTCA GAAACCCAAG GCACCAGTGG GGCATTTTTA CGAACCCCAG
    GCTCCCTCTG CTGAGGTGGA GATGACATCC TATGTGCTCC TCGCTTATCT CACGGCCCAG
    CCAGCCCCAA CCTCGGAGGA CCTGACCTCT GCAACCAACA TCGTGAAGTG GATCACGAAG
    CAGCAGAATG CCCAGGGCGG TTTCTCCTCC ACCCAGGACA CAGTGGTGGC TCTCCATGCT
    CTGTCCAAAT ATGGAGCAGC CACATTTACC AGGACTGGGA AGGCTGCACA GGTGACTATC
    CAGTCTTCAG GGACATTTTC CAGCAAATTC CAAGTGGACA ACAACAACCG CCTGTTACTG
    CAGCAGGTCT CATTGCCAGA GCTGCCTGGG GAATACAGCA TGAAAGTGAC AGGAGAAGGA
    TGTGTCTACC TCCAGACATC CTTGAAATAC AATATTCTCC CAGAAAAGGA AGAGTTCCCC
    TTTGCTTTAG GAGTGCAGAC TCTGCCTCAA ACTTGTGATG AACCCAAAGC CCACACCAGC
    TTCCAAATCT CCCTAAGTGT CAGTTACACA GGGAGCCGCT CTGCCTCCAA CATGGCGATC
    GTTGATGTGA AGATGGTCTC TGGCTTCATT CCCCTGAAGC CAACAGTGAA AATGCTTGAA
    AGATCTAACC ATGTGAGCCG GACAGAAGTC AGCAGCAACC ATGTCTTGAT TTACCTTGAT
    AAGGTGTCAA ATCAGACACT GAGCTTGTTC TTCACGGTTC TGCAAGATGT CCCAGTAAGA
    GATCTGAAAC CAGCCATAGT GAAAGTCTAT GATTACTACG AGACGGATGA GTTTGCAATT
    GCTGAGTACA ATGCTCCTTG CAGCAAAGAT CTTGGAAATG CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001334352.2
    CDS120..4544
    Translation

    Target ORF information:

    RefSeq Version NM_001347423.2
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha-2-macroglobulin (A2M), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001347423.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGGGAAGA ACAAACTCCT TCATCCAAGT CTGGTTCTTC TCCTCTTGGT CCTCCTGCCC 
    ACAGACGCCT CAGTCTCTGG AAAACCGCAG TATATGGTTC TGGTCCCCTC CCTGCTCCAC
    ACTGAGACCA CTGAGAAGGG CTGTGTCCTT CTGAGCTACC TGAATGAGAC AGTGACTGTA
    AGTGCTTCCT TGGAGTCTGT CAGGGGAAAC AGGAGCCTCT TCACTGACCT GGAGGCGGAG
    AATGACGTAC TCCACTGTGT CGCCTTCGCT GTCCCAAAGT CTTCATCCAA TGAGGAGGTA
    ATGTTCCTCA CTGTCCAAGT GAAAGGACCA ACCCAAGAAT TTAAGAAGCG GACCACAGTG
    ATGGTTAAGA ACGAGGACAG TCTGGTCTTT GTCCAGACAG ACAAATCAAT CTACAAACCA
    GGGCAGACAG TGAAATTTCG TGTTGTCTCC ATGGATGAAA ACTTTCACCC CCTGAATGAG
    TTGATTCCAC TAGTATACAT TCAGGATCCC AAAGGAAATC GCATCGCACA ATGGCAGAGT
    TTCCAGTTAG AGGGTGGCCT CAAGCAATTT TCTTTTCCCC TCTCATCAGA GCCCTTCCAG
    GGCTCCTACA AGGTGGTGGT ACAGAAGAAA TCAGGTGGAA GGACAGAGCA CCCTTTCACC
    GTGGAGGAAT TTGTTCTTCC CAAGTTTGAA GTACAAGTAA CAGTGCCAAA GATAATCACC
    ATCTTGGAAG AAGAGATGAA TGTATCAGTG TGTGGCCTAT ACACATATGG GAAGCCTGTC
    CCTGGACATG TGACTGTGAG CATTTGCAGA AAGTATAGTG ACGCTTCCGA CTGCCACGGT
    GAAGATTCAC AGGCTTTCTG TGAGAAATTC AGTGGACAGC TAAACAGCCA TGGCTGCTTC
    TATCAGCAAG TAAAAACCAA GGTCTTCCAG CTGAAGAGGA AGGAGTATGA AATGAAACTT
    CACACTGAGG CCCAGATCCA AGAAGAAGGA ACAGTGGTGG AATTGACTGG AAGGCAGTCC
    AGTGAAATCA CAAGAACCAT AACCAAACTC TCATTTGTGA AAGTGGACTC ACACTTTCGA
    CAGGGAATTC CCTTCTTTGG GCAGGTGCGC CTAGTAGATG GGAAAGGCGT CCCTATACCA
    AATAAAGTCA TATTCATCAG AGGAAATGAA GCAAACTATT ACTCCAATGC TACCACGGAT
    GAGCATGGCC TTGTACAGTT CTCTATCAAC ACCACCAATG TTATGGGTAC CTCTCTTACT
    GTTAGGGTCA ATTACAAGGA TCGTAGTCCC TGTTACGGCT ACCAGTGGGT GTCAGAAGAA
    CACGAAGAGG CACATCACAC TGCTTATCTT GTGTTCTCCC CAAGCAAGAG CTTTGTCCAC
    CTTGAGCCCA TGTCTCATGA ACTACCCTGT GGCCATACTC AGACAGTCCA GGCACATTAT
    ATTCTGAATG GAGGCACCCT GCTGGGGCTG AAGAAGCTCT CCTTCTATTA TCTGATAATG
    GCAAAGGGAG GCATTGTCCG AACTGGGACT CATGGACTGC TTGTGAAGCA GGAAGACATG
    AAGGGCCATT TTTCCATCTC AATCCCTGTG AAGTCAGACA TTGCTCCTGT CGCTCGGTTG
    CTCATCTATG CTGTTTTACC TACCGGGGAC GTGATTGGGG ATTCTGCAAA ATATGATGTT
    GAAAATTGTC TGGCCAACAA GGTGGATTTG AGCTTCAGCC CATCACAAAG TCTCCCAGCC
    TCACACGCCC ACCTGCGAGT CACAGCGGCT CCTCAGTCCG TCTGCGCCCT CCGTGCTGTG
    GACCAAAGCG TGCTGCTCAT GAAGCCTGAT GCTGAGCTCT CGGCGTCCTC GGTTTACAAC
    CTGCTACCAG AAAAGGACCT CACTGGCTTC CCTGGGCCTT TGAATGACCA GGACAATGAA
    GACTGCATCA ATCGTCATAA TGTCTATATT AATGGAATCA CATATACTCC AGTATCAAGT
    ACAAATGAAA AGGATATGTA CAGCTTCCTA GAGGACATGG GCTTAAAGGC ATTCACCAAC
    TCAAAGATTC GTAAACCCAA AATGTGTCCA CAGCTTCAAC AGTATGAAAT GCATGGACCT
    GAAGGTCTAC GTGTAGGTTT TTATGAGTCA GATGTAATGG GAAGAGGCCA TGCACGCCTG
    GTGCATGTTG AAGAGCCTCA CACGGAGACC GTACGAAAGT ACTTCCCTGA GACATGGATC
    TGGGATTTGG TGGTGGTAAA CTCAGCAGGT GTGGCTGAGG TAGGAGTAAC AGTCCCTGAC
    ACCATCACCG AGTGGAAGGC AGGGGCCTTC TGCCTGTCTG AAGATGCTGG ACTTGGTATC
    TCTTCCACTG CCTCTCTCCG AGCCTTCCAG CCCTTCTTTG TGGAGCTCAC AATGCCTTAC
    TCTGTGATTC GTGGAGAGGC CTTCACACTC AAGGCCACGG TCCTAAACTA CCTTCCCAAA
    TGCATCCGGG TCAGTGTGCA GCTGGAAGCC TCTCCCGCCT TCCTAGCTGT CCCAGTGGAG
    AAGGAACAAG CGCCTCACTG CATCTGTGCA AACGGGCGGC AAACTGTGTC CTGGGCAGTA
    ACCCCAAAGT CATTAGGAAA TGTGAATTTC ACTGTGAGCG CAGAGGCACT AGAGTCTCAA
    GAGCTGTGTG GGACTGAGGT GCCTTCAGTT CCTGAACACG GAAGGAAAGA CACAGTCATC
    AAGCCTCTGT TGGTTGAACC TGAAGGACTA GAGAAGGAAA CAACATTCAA CTCCCTACTT
    TGTCCATCAG GTGGTGAGGT TTCTGAAGAA TTATCCCTGA AACTGCCACC AAATGTGGTA
    GAAGAATCTG CCCGAGCTTC TGTCTCAGTT TTGGGAGACA TATTAGGCTC TGCCATGCAA
    AACACACAAA ATCTTCTCCA GATGCCCTAT GGCTGTGGAG AGCAGAATAT GGTCCTCTTT
    GCTCCTAACA TCTATGTACT GGATTATCTA AATGAAACAC AGCAGCTTAC TCCAGAGATC
    AAGTCCAAGG CCATTGGCTA TCTCAACACT GGTTACCAGA GACAGTTGAA CTACAAACAC
    TATGATGGCT CCTACAGCAC CTTTGGGGAG CGATATGGCA GGAACCAGGG CAACACCTGG
    CTCACAGCCT TTGTTCTGAA GACTTTTGCC CAAGCTCGAG CCTACATCTT CATCGATGAA
    GCACACATTA CCCAAGCCCT CATATGGCTC TCCCAGAGGC AGAAGGACAA TGGCTGTTTC
    AGGAGCTCTG GGTCACTGCT CAACAATGCC ATAAAGGGAG GAGTAGAAGA TGAAGTGACC
    CTCTCCGCCT ATATCACCAT CGCCCTTCTG GAGATTCCTC TCACAGTCAC TCACCCTGTT
    GTCCGCAATG CCCTGTTTTG CCTGGAGTCA GCCTGGAAGA CAGCACAAGA AGGGGACCAT
    GGCAGCCATG TATATACCAA AGCACTGCTG GCCTATGCTT TTGCCCTGGC AGGTAACCAG
    GACAAGAGGA AGGAAGTACT CAAGTCACTT AATGAGGAAG CTGTGAAGAA AGACAACTCT
    GTCCATTGGG AGCGCCCTCA GAAACCCAAG GCACCAGTGG GGCATTTTTA CGAACCCCAG
    GCTCCCTCTG CTGAGGTGGA GATGACATCC TATGTGCTCC TCGCTTATCT CACGGCCCAG
    CCAGCCCCAA CCTCGGAGGA CCTGACCTCT GCAACCAACA TCGTGAAGTG GATCACGAAG
    CAGCAGAATG CCCAGGGCGG TTTCTCCTCC ACCCAGGACA CAGTGGTGGC TCTCCATGCT
    CTGTCCAAAT ATGGAGCAGC CACATTTACC AGGACTGGGA AGGCTGCACA GGTGACTATC
    CAGTCTTCAG GGACATTTTC CAGCAAATTC CAAGTGGACA ACAACAACCG CCTGTTACTG
    CAGCAGGTCT CATTGCCAGA GCTGCCTGGG GAATACAGCA TGAAAGTGAC AGGAGAAGGA
    TGTGTCTACC TCCAGACATC CTTGAAATAC AATATTCTCC CAGAAAAGGA AGAGTTCCCC
    TTTGCTTTAG GAGTGCAGAC TCTGCCTCAA ACTTGTGATG AACCCAAAGC CCACACCAGC
    TTCCAAATCT CCCTAAGTGT CAGTTACACA GGGAGCCGCT CTGCCTCCAA CATGGCGATC
    GTTGATGTGA AGATGGTCTC TGGCTTCATT CCCCTGAAGC CAACAGTGAA AATGCTTGAA
    AGATCTAACC ATGTGAGCCG GACAGAAGTC AGCAGCAACC ATGTCTTGAT TTACCTTGAT
    AAGGTGTCAA ATCAGACACT GAGCTTGTTC TTCACGGTTC TGCAAGATGT CCCAGTAAGA
    GATCTGAAAC CAGCCATAGT GAAAGTCTAT GATTACTACG AGACGGATGA GTTTGCAATT
    GCTGAGTACA ATGCTCCTTG CAGCAAAGAT CTTGGAAATG CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108400
    Clone ID Related Accession (Same CDS sequence) NM_001347423.2 , NM_000014.6
    Accession Version NM_000014.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4425bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-29
    Organism Homo sapiens(human)
    Product alpha-2-macroglobulin isoform a precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC007436.1. On Nov 30, 2019 this sequence version replaced NM_000014.5. Transcript Variant: This variant (1) encodes the longest isoform (a). Both variants 1 and 2 encode isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC040071.1, AB209614.2 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGGGAAGA ACAAACTCCT TCATCCAAGT CTGGTTCTTC TCCTCTTGGT CCTCCTGCCC 
    ACAGACGCCT CAGTCTCTGG AAAACCGCAG TATATGGTTC TGGTCCCCTC CCTGCTCCAC
    ACTGAGACCA CTGAGAAGGG CTGTGTCCTT CTGAGCTACC TGAATGAGAC AGTGACTGTA
    AGTGCTTCCT TGGAGTCTGT CAGGGGAAAC AGGAGCCTCT TCACTGACCT GGAGGCGGAG
    AATGACGTAC TCCACTGTGT CGCCTTCGCT GTCCCAAAGT CTTCATCCAA TGAGGAGGTA
    ATGTTCCTCA CTGTCCAAGT GAAAGGACCA ACCCAAGAAT TTAAGAAGCG GACCACAGTG
    ATGGTTAAGA ACGAGGACAG TCTGGTCTTT GTCCAGACAG ACAAATCAAT CTACAAACCA
    GGGCAGACAG TGAAATTTCG TGTTGTCTCC ATGGATGAAA ACTTTCACCC CCTGAATGAG
    TTGATTCCAC TAGTATACAT TCAGGATCCC AAAGGAAATC GCATCGCACA ATGGCAGAGT
    TTCCAGTTAG AGGGTGGCCT CAAGCAATTT TCTTTTCCCC TCTCATCAGA GCCCTTCCAG
    GGCTCCTACA AGGTGGTGGT ACAGAAGAAA TCAGGTGGAA GGACAGAGCA CCCTTTCACC
    GTGGAGGAAT TTGTTCTTCC CAAGTTTGAA GTACAAGTAA CAGTGCCAAA GATAATCACC
    ATCTTGGAAG AAGAGATGAA TGTATCAGTG TGTGGCCTAT ACACATATGG GAAGCCTGTC
    CCTGGACATG TGACTGTGAG CATTTGCAGA AAGTATAGTG ACGCTTCCGA CTGCCACGGT
    GAAGATTCAC AGGCTTTCTG TGAGAAATTC AGTGGACAGC TAAACAGCCA TGGCTGCTTC
    TATCAGCAAG TAAAAACCAA GGTCTTCCAG CTGAAGAGGA AGGAGTATGA AATGAAACTT
    CACACTGAGG CCCAGATCCA AGAAGAAGGA ACAGTGGTGG AATTGACTGG AAGGCAGTCC
    AGTGAAATCA CAAGAACCAT AACCAAACTC TCATTTGTGA AAGTGGACTC ACACTTTCGA
    CAGGGAATTC CCTTCTTTGG GCAGGTGCGC CTAGTAGATG GGAAAGGCGT CCCTATACCA
    AATAAAGTCA TATTCATCAG AGGAAATGAA GCAAACTATT ACTCCAATGC TACCACGGAT
    GAGCATGGCC TTGTACAGTT CTCTATCAAC ACCACCAATG TTATGGGTAC CTCTCTTACT
    GTTAGGGTCA ATTACAAGGA TCGTAGTCCC TGTTACGGCT ACCAGTGGGT GTCAGAAGAA
    CACGAAGAGG CACATCACAC TGCTTATCTT GTGTTCTCCC CAAGCAAGAG CTTTGTCCAC
    CTTGAGCCCA TGTCTCATGA ACTACCCTGT GGCCATACTC AGACAGTCCA GGCACATTAT
    ATTCTGAATG GAGGCACCCT GCTGGGGCTG AAGAAGCTCT CCTTCTATTA TCTGATAATG
    GCAAAGGGAG GCATTGTCCG AACTGGGACT CATGGACTGC TTGTGAAGCA GGAAGACATG
    AAGGGCCATT TTTCCATCTC AATCCCTGTG AAGTCAGACA TTGCTCCTGT CGCTCGGTTG
    CTCATCTATG CTGTTTTACC TACCGGGGAC GTGATTGGGG ATTCTGCAAA ATATGATGTT
    GAAAATTGTC TGGCCAACAA GGTGGATTTG AGCTTCAGCC CATCACAAAG TCTCCCAGCC
    TCACACGCCC ACCTGCGAGT CACAGCGGCT CCTCAGTCCG TCTGCGCCCT CCGTGCTGTG
    GACCAAAGCG TGCTGCTCAT GAAGCCTGAT GCTGAGCTCT CGGCGTCCTC GGTTTACAAC
    CTGCTACCAG AAAAGGACCT CACTGGCTTC CCTGGGCCTT TGAATGACCA GGACAATGAA
    GACTGCATCA ATCGTCATAA TGTCTATATT AATGGAATCA CATATACTCC AGTATCAAGT
    ACAAATGAAA AGGATATGTA CAGCTTCCTA GAGGACATGG GCTTAAAGGC ATTCACCAAC
    TCAAAGATTC GTAAACCCAA AATGTGTCCA CAGCTTCAAC AGTATGAAAT GCATGGACCT
    GAAGGTCTAC GTGTAGGTTT TTATGAGTCA GATGTAATGG GAAGAGGCCA TGCACGCCTG
    GTGCATGTTG AAGAGCCTCA CACGGAGACC GTACGAAAGT ACTTCCCTGA GACATGGATC
    TGGGATTTGG TGGTGGTAAA CTCAGCAGGT GTGGCTGAGG TAGGAGTAAC AGTCCCTGAC
    ACCATCACCG AGTGGAAGGC AGGGGCCTTC TGCCTGTCTG AAGATGCTGG ACTTGGTATC
    TCTTCCACTG CCTCTCTCCG AGCCTTCCAG CCCTTCTTTG TGGAGCTCAC AATGCCTTAC
    TCTGTGATTC GTGGAGAGGC CTTCACACTC AAGGCCACGG TCCTAAACTA CCTTCCCAAA
    TGCATCCGGG TCAGTGTGCA GCTGGAAGCC TCTCCCGCCT TCCTAGCTGT CCCAGTGGAG
    AAGGAACAAG CGCCTCACTG CATCTGTGCA AACGGGCGGC AAACTGTGTC CTGGGCAGTA
    ACCCCAAAGT CATTAGGAAA TGTGAATTTC ACTGTGAGCG CAGAGGCACT AGAGTCTCAA
    GAGCTGTGTG GGACTGAGGT GCCTTCAGTT CCTGAACACG GAAGGAAAGA CACAGTCATC
    AAGCCTCTGT TGGTTGAACC TGAAGGACTA GAGAAGGAAA CAACATTCAA CTCCCTACTT
    TGTCCATCAG GTGGTGAGGT TTCTGAAGAA TTATCCCTGA AACTGCCACC AAATGTGGTA
    GAAGAATCTG CCCGAGCTTC TGTCTCAGTT TTGGGAGACA TATTAGGCTC TGCCATGCAA
    AACACACAAA ATCTTCTCCA GATGCCCTAT GGCTGTGGAG AGCAGAATAT GGTCCTCTTT
    GCTCCTAACA TCTATGTACT GGATTATCTA AATGAAACAC AGCAGCTTAC TCCAGAGATC
    AAGTCCAAGG CCATTGGCTA TCTCAACACT GGTTACCAGA GACAGTTGAA CTACAAACAC
    TATGATGGCT CCTACAGCAC CTTTGGGGAG CGATATGGCA GGAACCAGGG CAACACCTGG
    CTCACAGCCT TTGTTCTGAA GACTTTTGCC CAAGCTCGAG CCTACATCTT CATCGATGAA
    GCACACATTA CCCAAGCCCT CATATGGCTC TCCCAGAGGC AGAAGGACAA TGGCTGTTTC
    AGGAGCTCTG GGTCACTGCT CAACAATGCC ATAAAGGGAG GAGTAGAAGA TGAAGTGACC
    CTCTCCGCCT ATATCACCAT CGCCCTTCTG GAGATTCCTC TCACAGTCAC TCACCCTGTT
    GTCCGCAATG CCCTGTTTTG CCTGGAGTCA GCCTGGAAGA CAGCACAAGA AGGGGACCAT
    GGCAGCCATG TATATACCAA AGCACTGCTG GCCTATGCTT TTGCCCTGGC AGGTAACCAG
    GACAAGAGGA AGGAAGTACT CAAGTCACTT AATGAGGAAG CTGTGAAGAA AGACAACTCT
    GTCCATTGGG AGCGCCCTCA GAAACCCAAG GCACCAGTGG GGCATTTTTA CGAACCCCAG
    GCTCCCTCTG CTGAGGTGGA GATGACATCC TATGTGCTCC TCGCTTATCT CACGGCCCAG
    CCAGCCCCAA CCTCGGAGGA CCTGACCTCT GCAACCAACA TCGTGAAGTG GATCACGAAG
    CAGCAGAATG CCCAGGGCGG TTTCTCCTCC ACCCAGGACA CAGTGGTGGC TCTCCATGCT
    CTGTCCAAAT ATGGAGCAGC CACATTTACC AGGACTGGGA AGGCTGCACA GGTGACTATC
    CAGTCTTCAG GGACATTTTC CAGCAAATTC CAAGTGGACA ACAACAACCG CCTGTTACTG
    CAGCAGGTCT CATTGCCAGA GCTGCCTGGG GAATACAGCA TGAAAGTGAC AGGAGAAGGA
    TGTGTCTACC TCCAGACATC CTTGAAATAC AATATTCTCC CAGAAAAGGA AGAGTTCCCC
    TTTGCTTTAG GAGTGCAGAC TCTGCCTCAA ACTTGTGATG AACCCAAAGC CCACACCAGC
    TTCCAAATCT CCCTAAGTGT CAGTTACACA GGGAGCCGCT CTGCCTCCAA CATGGCGATC
    GTTGATGTGA AGATGGTCTC TGGCTTCATT CCCCTGAAGC CAACAGTGAA AATGCTTGAA
    AGATCTAACC ATGTGAGCCG GACAGAAGTC AGCAGCAACC ATGTCTTGAT TTACCTTGAT
    AAGGTGTCAA ATCAGACACT GAGCTTGTTC TTCACGGTTC TGCAAGATGT CCCAGTAAGA
    GATCTGAAAC CAGCCATAGT GAAAGTCTAT GATTACTACG AGACGGATGA GTTTGCAATT
    GCTGAGTACA ATGCTCCTTG CAGCAAAGAT CTTGGAAATG CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000005.3
    CDS71..4495
    Translation

    Target ORF information:

    RefSeq Version NM_000014.6
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha-2-macroglobulin (A2M), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000014.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGGGAAGA ACAAACTCCT TCATCCAAGT CTGGTTCTTC TCCTCTTGGT CCTCCTGCCC 
    ACAGACGCCT CAGTCTCTGG AAAACCGCAG TATATGGTTC TGGTCCCCTC CCTGCTCCAC
    ACTGAGACCA CTGAGAAGGG CTGTGTCCTT CTGAGCTACC TGAATGAGAC AGTGACTGTA
    AGTGCTTCCT TGGAGTCTGT CAGGGGAAAC AGGAGCCTCT TCACTGACCT GGAGGCGGAG
    AATGACGTAC TCCACTGTGT CGCCTTCGCT GTCCCAAAGT CTTCATCCAA TGAGGAGGTA
    ATGTTCCTCA CTGTCCAAGT GAAAGGACCA ACCCAAGAAT TTAAGAAGCG GACCACAGTG
    ATGGTTAAGA ACGAGGACAG TCTGGTCTTT GTCCAGACAG ACAAATCAAT CTACAAACCA
    GGGCAGACAG TGAAATTTCG TGTTGTCTCC ATGGATGAAA ACTTTCACCC CCTGAATGAG
    TTGATTCCAC TAGTATACAT TCAGGATCCC AAAGGAAATC GCATCGCACA ATGGCAGAGT
    TTCCAGTTAG AGGGTGGCCT CAAGCAATTT TCTTTTCCCC TCTCATCAGA GCCCTTCCAG
    GGCTCCTACA AGGTGGTGGT ACAGAAGAAA TCAGGTGGAA GGACAGAGCA CCCTTTCACC
    GTGGAGGAAT TTGTTCTTCC CAAGTTTGAA GTACAAGTAA CAGTGCCAAA GATAATCACC
    ATCTTGGAAG AAGAGATGAA TGTATCAGTG TGTGGCCTAT ACACATATGG GAAGCCTGTC
    CCTGGACATG TGACTGTGAG CATTTGCAGA AAGTATAGTG ACGCTTCCGA CTGCCACGGT
    GAAGATTCAC AGGCTTTCTG TGAGAAATTC AGTGGACAGC TAAACAGCCA TGGCTGCTTC
    TATCAGCAAG TAAAAACCAA GGTCTTCCAG CTGAAGAGGA AGGAGTATGA AATGAAACTT
    CACACTGAGG CCCAGATCCA AGAAGAAGGA ACAGTGGTGG AATTGACTGG AAGGCAGTCC
    AGTGAAATCA CAAGAACCAT AACCAAACTC TCATTTGTGA AAGTGGACTC ACACTTTCGA
    CAGGGAATTC CCTTCTTTGG GCAGGTGCGC CTAGTAGATG GGAAAGGCGT CCCTATACCA
    AATAAAGTCA TATTCATCAG AGGAAATGAA GCAAACTATT ACTCCAATGC TACCACGGAT
    GAGCATGGCC TTGTACAGTT CTCTATCAAC ACCACCAATG TTATGGGTAC CTCTCTTACT
    GTTAGGGTCA ATTACAAGGA TCGTAGTCCC TGTTACGGCT ACCAGTGGGT GTCAGAAGAA
    CACGAAGAGG CACATCACAC TGCTTATCTT GTGTTCTCCC CAAGCAAGAG CTTTGTCCAC
    CTTGAGCCCA TGTCTCATGA ACTACCCTGT GGCCATACTC AGACAGTCCA GGCACATTAT
    ATTCTGAATG GAGGCACCCT GCTGGGGCTG AAGAAGCTCT CCTTCTATTA TCTGATAATG
    GCAAAGGGAG GCATTGTCCG AACTGGGACT CATGGACTGC TTGTGAAGCA GGAAGACATG
    AAGGGCCATT TTTCCATCTC AATCCCTGTG AAGTCAGACA TTGCTCCTGT CGCTCGGTTG
    CTCATCTATG CTGTTTTACC TACCGGGGAC GTGATTGGGG ATTCTGCAAA ATATGATGTT
    GAAAATTGTC TGGCCAACAA GGTGGATTTG AGCTTCAGCC CATCACAAAG TCTCCCAGCC
    TCACACGCCC ACCTGCGAGT CACAGCGGCT CCTCAGTCCG TCTGCGCCCT CCGTGCTGTG
    GACCAAAGCG TGCTGCTCAT GAAGCCTGAT GCTGAGCTCT CGGCGTCCTC GGTTTACAAC
    CTGCTACCAG AAAAGGACCT CACTGGCTTC CCTGGGCCTT TGAATGACCA GGACAATGAA
    GACTGCATCA ATCGTCATAA TGTCTATATT AATGGAATCA CATATACTCC AGTATCAAGT
    ACAAATGAAA AGGATATGTA CAGCTTCCTA GAGGACATGG GCTTAAAGGC ATTCACCAAC
    TCAAAGATTC GTAAACCCAA AATGTGTCCA CAGCTTCAAC AGTATGAAAT GCATGGACCT
    GAAGGTCTAC GTGTAGGTTT TTATGAGTCA GATGTAATGG GAAGAGGCCA TGCACGCCTG
    GTGCATGTTG AAGAGCCTCA CACGGAGACC GTACGAAAGT ACTTCCCTGA GACATGGATC
    TGGGATTTGG TGGTGGTAAA CTCAGCAGGT GTGGCTGAGG TAGGAGTAAC AGTCCCTGAC
    ACCATCACCG AGTGGAAGGC AGGGGCCTTC TGCCTGTCTG AAGATGCTGG ACTTGGTATC
    TCTTCCACTG CCTCTCTCCG AGCCTTCCAG CCCTTCTTTG TGGAGCTCAC AATGCCTTAC
    TCTGTGATTC GTGGAGAGGC CTTCACACTC AAGGCCACGG TCCTAAACTA CCTTCCCAAA
    TGCATCCGGG TCAGTGTGCA GCTGGAAGCC TCTCCCGCCT TCCTAGCTGT CCCAGTGGAG
    AAGGAACAAG CGCCTCACTG CATCTGTGCA AACGGGCGGC AAACTGTGTC CTGGGCAGTA
    ACCCCAAAGT CATTAGGAAA TGTGAATTTC ACTGTGAGCG CAGAGGCACT AGAGTCTCAA
    GAGCTGTGTG GGACTGAGGT GCCTTCAGTT CCTGAACACG GAAGGAAAGA CACAGTCATC
    AAGCCTCTGT TGGTTGAACC TGAAGGACTA GAGAAGGAAA CAACATTCAA CTCCCTACTT
    TGTCCATCAG GTGGTGAGGT TTCTGAAGAA TTATCCCTGA AACTGCCACC AAATGTGGTA
    GAAGAATCTG CCCGAGCTTC TGTCTCAGTT TTGGGAGACA TATTAGGCTC TGCCATGCAA
    AACACACAAA ATCTTCTCCA GATGCCCTAT GGCTGTGGAG AGCAGAATAT GGTCCTCTTT
    GCTCCTAACA TCTATGTACT GGATTATCTA AATGAAACAC AGCAGCTTAC TCCAGAGATC
    AAGTCCAAGG CCATTGGCTA TCTCAACACT GGTTACCAGA GACAGTTGAA CTACAAACAC
    TATGATGGCT CCTACAGCAC CTTTGGGGAG CGATATGGCA GGAACCAGGG CAACACCTGG
    CTCACAGCCT TTGTTCTGAA GACTTTTGCC CAAGCTCGAG CCTACATCTT CATCGATGAA
    GCACACATTA CCCAAGCCCT CATATGGCTC TCCCAGAGGC AGAAGGACAA TGGCTGTTTC
    AGGAGCTCTG GGTCACTGCT CAACAATGCC ATAAAGGGAG GAGTAGAAGA TGAAGTGACC
    CTCTCCGCCT ATATCACCAT CGCCCTTCTG GAGATTCCTC TCACAGTCAC TCACCCTGTT
    GTCCGCAATG CCCTGTTTTG CCTGGAGTCA GCCTGGAAGA CAGCACAAGA AGGGGACCAT
    GGCAGCCATG TATATACCAA AGCACTGCTG GCCTATGCTT TTGCCCTGGC AGGTAACCAG
    GACAAGAGGA AGGAAGTACT CAAGTCACTT AATGAGGAAG CTGTGAAGAA AGACAACTCT
    GTCCATTGGG AGCGCCCTCA GAAACCCAAG GCACCAGTGG GGCATTTTTA CGAACCCCAG
    GCTCCCTCTG CTGAGGTGGA GATGACATCC TATGTGCTCC TCGCTTATCT CACGGCCCAG
    CCAGCCCCAA CCTCGGAGGA CCTGACCTCT GCAACCAACA TCGTGAAGTG GATCACGAAG
    CAGCAGAATG CCCAGGGCGG TTTCTCCTCC ACCCAGGACA CAGTGGTGGC TCTCCATGCT
    CTGTCCAAAT ATGGAGCAGC CACATTTACC AGGACTGGGA AGGCTGCACA GGTGACTATC
    CAGTCTTCAG GGACATTTTC CAGCAAATTC CAAGTGGACA ACAACAACCG CCTGTTACTG
    CAGCAGGTCT CATTGCCAGA GCTGCCTGGG GAATACAGCA TGAAAGTGAC AGGAGAAGGA
    TGTGTCTACC TCCAGACATC CTTGAAATAC AATATTCTCC CAGAAAAGGA AGAGTTCCCC
    TTTGCTTTAG GAGTGCAGAC TCTGCCTCAA ACTTGTGATG AACCCAAAGC CCACACCAGC
    TTCCAAATCT CCCTAAGTGT CAGTTACACA GGGAGCCGCT CTGCCTCCAA CATGGCGATC
    GTTGATGTGA AGATGGTCTC TGGCTTCATT CCCCTGAAGC CAACAGTGAA AATGCTTGAA
    AGATCTAACC ATGTGAGCCG GACAGAAGTC AGCAGCAACC ATGTCTTGAT TTACCTTGAT
    AAGGTGTCAA ATCAGACACT GAGCTTGTTC TTCACGGTTC TGCAAGATGT CCCAGTAAGA
    GATCTGAAAC CAGCCATAGT GAAAGTCTAT GATTACTACG AGACGGATGA GTTTGCAATT
    GCTGAGTACA ATGCTCCTTG CAGCAAAGAT CTTGGAAATG CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu109274
    Clone ID Related Accession (Same CDS sequence) NM_001347424.2
    Accession Version NM_001347424.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4125bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-29
    Organism Homo sapiens(human)
    Product alpha-2-macroglobulin isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC007436.1. On Nov 30, 2019 this sequence version replaced NM_001347424.1. Transcript Variant: This variant (3) contains an alternate splice site in the 5' region, and it thus differs in its 5' UTR and initiates translation from a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.221045.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTTCCTCA CTGTCCAAGT GAAAGGACCA ACCCAAGAAT TTAAGAAGCG GACCACAGTG 
    ATGGTTAAGA ACGAGGACAG TCTGGTCTTT GTCCAGACAG ACAAATCAAT CTACAAACCA
    GGGCAGACAG TGAAATTTCG TGTTGTCTCC ATGGATGAAA ACTTTCACCC CCTGAATGAG
    TTGATTCCAC TAGTATACAT TCAGGATCCC AAAGGAAATC GCATCGCACA ATGGCAGAGT
    TTCCAGTTAG AGGGTGGCCT CAAGCAATTT TCTTTTCCCC TCTCATCAGA GCCCTTCCAG
    GGCTCCTACA AGGTGGTGGT ACAGAAGAAA TCAGGTGGAA GGACAGAGCA CCCTTTCACC
    GTGGAGGAAT TTGTTCTTCC CAAGTTTGAA GTACAAGTAA CAGTGCCAAA GATAATCACC
    ATCTTGGAAG AAGAGATGAA TGTATCAGTG TGTGGCCTAT ACACATATGG GAAGCCTGTC
    CCTGGACATG TGACTGTGAG CATTTGCAGA AAGTATAGTG ACGCTTCCGA CTGCCACGGT
    GAAGATTCAC AGGCTTTCTG TGAGAAATTC AGTGGACAGC TAAACAGCCA TGGCTGCTTC
    TATCAGCAAG TAAAAACCAA GGTCTTCCAG CTGAAGAGGA AGGAGTATGA AATGAAACTT
    CACACTGAGG CCCAGATCCA AGAAGAAGGA ACAGTGGTGG AATTGACTGG AAGGCAGTCC
    AGTGAAATCA CAAGAACCAT AACCAAACTC TCATTTGTGA AAGTGGACTC ACACTTTCGA
    CAGGGAATTC CCTTCTTTGG GCAGGTGCGC CTAGTAGATG GGAAAGGCGT CCCTATACCA
    AATAAAGTCA TATTCATCAG AGGAAATGAA GCAAACTATT ACTCCAATGC TACCACGGAT
    GAGCATGGCC TTGTACAGTT CTCTATCAAC ACCACCAATG TTATGGGTAC CTCTCTTACT
    GTTAGGGTCA ATTACAAGGA TCGTAGTCCC TGTTACGGCT ACCAGTGGGT GTCAGAAGAA
    CACGAAGAGG CACATCACAC TGCTTATCTT GTGTTCTCCC CAAGCAAGAG CTTTGTCCAC
    CTTGAGCCCA TGTCTCATGA ACTACCCTGT GGCCATACTC AGACAGTCCA GGCACATTAT
    ATTCTGAATG GAGGCACCCT GCTGGGGCTG AAGAAGCTCT CCTTCTATTA TCTGATAATG
    GCAAAGGGAG GCATTGTCCG AACTGGGACT CATGGACTGC TTGTGAAGCA GGAAGACATG
    AAGGGCCATT TTTCCATCTC AATCCCTGTG AAGTCAGACA TTGCTCCTGT CGCTCGGTTG
    CTCATCTATG CTGTTTTACC TACCGGGGAC GTGATTGGGG ATTCTGCAAA ATATGATGTT
    GAAAATTGTC TGGCCAACAA GGTGGATTTG AGCTTCAGCC CATCACAAAG TCTCCCAGCC
    TCACACGCCC ACCTGCGAGT CACAGCGGCT CCTCAGTCCG TCTGCGCCCT CCGTGCTGTG
    GACCAAAGCG TGCTGCTCAT GAAGCCTGAT GCTGAGCTCT CGGCGTCCTC GGTTTACAAC
    CTGCTACCAG AAAAGGACCT CACTGGCTTC CCTGGGCCTT TGAATGACCA GGACAATGAA
    GACTGCATCA ATCGTCATAA TGTCTATATT AATGGAATCA CATATACTCC AGTATCAAGT
    ACAAATGAAA AGGATATGTA CAGCTTCCTA GAGGACATGG GCTTAAAGGC ATTCACCAAC
    TCAAAGATTC GTAAACCCAA AATGTGTCCA CAGCTTCAAC AGTATGAAAT GCATGGACCT
    GAAGGTCTAC GTGTAGGTTT TTATGAGTCA GATGTAATGG GAAGAGGCCA TGCACGCCTG
    GTGCATGTTG AAGAGCCTCA CACGGAGACC GTACGAAAGT ACTTCCCTGA GACATGGATC
    TGGGATTTGG TGGTGGTAAA CTCAGCAGGT GTGGCTGAGG TAGGAGTAAC AGTCCCTGAC
    ACCATCACCG AGTGGAAGGC AGGGGCCTTC TGCCTGTCTG AAGATGCTGG ACTTGGTATC
    TCTTCCACTG CCTCTCTCCG AGCCTTCCAG CCCTTCTTTG TGGAGCTCAC AATGCCTTAC
    TCTGTGATTC GTGGAGAGGC CTTCACACTC AAGGCCACGG TCCTAAACTA CCTTCCCAAA
    TGCATCCGGG TCAGTGTGCA GCTGGAAGCC TCTCCCGCCT TCCTAGCTGT CCCAGTGGAG
    AAGGAACAAG CGCCTCACTG CATCTGTGCA AACGGGCGGC AAACTGTGTC CTGGGCAGTA
    ACCCCAAAGT CATTAGGAAA TGTGAATTTC ACTGTGAGCG CAGAGGCACT AGAGTCTCAA
    GAGCTGTGTG GGACTGAGGT GCCTTCAGTT CCTGAACACG GAAGGAAAGA CACAGTCATC
    AAGCCTCTGT TGGTTGAACC TGAAGGACTA GAGAAGGAAA CAACATTCAA CTCCCTACTT
    TGTCCATCAG GTGGTGAGGT TTCTGAAGAA TTATCCCTGA AACTGCCACC AAATGTGGTA
    GAAGAATCTG CCCGAGCTTC TGTCTCAGTT TTGGGAGACA TATTAGGCTC TGCCATGCAA
    AACACACAAA ATCTTCTCCA GATGCCCTAT GGCTGTGGAG AGCAGAATAT GGTCCTCTTT
    GCTCCTAACA TCTATGTACT GGATTATCTA AATGAAACAC AGCAGCTTAC TCCAGAGATC
    AAGTCCAAGG CCATTGGCTA TCTCAACACT GGTTACCAGA GACAGTTGAA CTACAAACAC
    TATGATGGCT CCTACAGCAC CTTTGGGGAG CGATATGGCA GGAACCAGGG CAACACCTGG
    CTCACAGCCT TTGTTCTGAA GACTTTTGCC CAAGCTCGAG CCTACATCTT CATCGATGAA
    GCACACATTA CCCAAGCCCT CATATGGCTC TCCCAGAGGC AGAAGGACAA TGGCTGTTTC
    AGGAGCTCTG GGTCACTGCT CAACAATGCC ATAAAGGGAG GAGTAGAAGA TGAAGTGACC
    CTCTCCGCCT ATATCACCAT CGCCCTTCTG GAGATTCCTC TCACAGTCAC TCACCCTGTT
    GTCCGCAATG CCCTGTTTTG CCTGGAGTCA GCCTGGAAGA CAGCACAAGA AGGGGACCAT
    GGCAGCCATG TATATACCAA AGCACTGCTG GCCTATGCTT TTGCCCTGGC AGGTAACCAG
    GACAAGAGGA AGGAAGTACT CAAGTCACTT AATGAGGAAG CTGTGAAGAA AGACAACTCT
    GTCCATTGGG AGCGCCCTCA GAAACCCAAG GCACCAGTGG GGCATTTTTA CGAACCCCAG
    GCTCCCTCTG CTGAGGTGGA GATGACATCC TATGTGCTCC TCGCTTATCT CACGGCCCAG
    CCAGCCCCAA CCTCGGAGGA CCTGACCTCT GCAACCAACA TCGTGAAGTG GATCACGAAG
    CAGCAGAATG CCCAGGGCGG TTTCTCCTCC ACCCAGGACA CAGTGGTGGC TCTCCATGCT
    CTGTCCAAAT ATGGAGCAGC CACATTTACC AGGACTGGGA AGGCTGCACA GGTGACTATC
    CAGTCTTCAG GGACATTTTC CAGCAAATTC CAAGTGGACA ACAACAACCG CCTGTTACTG
    CAGCAGGTCT CATTGCCAGA GCTGCCTGGG GAATACAGCA TGAAAGTGAC AGGAGAAGGA
    TGTGTCTACC TCCAGACATC CTTGAAATAC AATATTCTCC CAGAAAAGGA AGAGTTCCCC
    TTTGCTTTAG GAGTGCAGAC TCTGCCTCAA ACTTGTGATG AACCCAAAGC CCACACCAGC
    TTCCAAATCT CCCTAAGTGT CAGTTACACA GGGAGCCGCT CTGCCTCCAA CATGGCGATC
    GTTGATGTGA AGATGGTCTC TGGCTTCATT CCCCTGAAGC CAACAGTGAA AATGCTTGAA
    AGATCTAACC ATGTGAGCCG GACAGAAGTC AGCAGCAACC ATGTCTTGAT TTACCTTGAT
    AAGGTGTCAA ATCAGACACT GAGCTTGTTC TTCACGGTTC TGCAAGATGT CCCAGTAAGA
    GATCTGAAAC CAGCCATAGT GAAAGTCTAT GATTACTACG AGACGGATGA GTTTGCAATT
    GCTGAGTACA ATGCTCCTTG CAGCAAAGAT CTTGGAAATG CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001334353.2
    CDS524..4648
    Translation

    Target ORF information:

    RefSeq Version NM_001347424.2
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha-2-macroglobulin (A2M), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001347424.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTTCCTCA CTGTCCAAGT GAAAGGACCA ACCCAAGAAT TTAAGAAGCG GACCACAGTG 
    ATGGTTAAGA ACGAGGACAG TCTGGTCTTT GTCCAGACAG ACAAATCAAT CTACAAACCA
    GGGCAGACAG TGAAATTTCG TGTTGTCTCC ATGGATGAAA ACTTTCACCC CCTGAATGAG
    TTGATTCCAC TAGTATACAT TCAGGATCCC AAAGGAAATC GCATCGCACA ATGGCAGAGT
    TTCCAGTTAG AGGGTGGCCT CAAGCAATTT TCTTTTCCCC TCTCATCAGA GCCCTTCCAG
    GGCTCCTACA AGGTGGTGGT ACAGAAGAAA TCAGGTGGAA GGACAGAGCA CCCTTTCACC
    GTGGAGGAAT TTGTTCTTCC CAAGTTTGAA GTACAAGTAA CAGTGCCAAA GATAATCACC
    ATCTTGGAAG AAGAGATGAA TGTATCAGTG TGTGGCCTAT ACACATATGG GAAGCCTGTC
    CCTGGACATG TGACTGTGAG CATTTGCAGA AAGTATAGTG ACGCTTCCGA CTGCCACGGT
    GAAGATTCAC AGGCTTTCTG TGAGAAATTC AGTGGACAGC TAAACAGCCA TGGCTGCTTC
    TATCAGCAAG TAAAAACCAA GGTCTTCCAG CTGAAGAGGA AGGAGTATGA AATGAAACTT
    CACACTGAGG CCCAGATCCA AGAAGAAGGA ACAGTGGTGG AATTGACTGG AAGGCAGTCC
    AGTGAAATCA CAAGAACCAT AACCAAACTC TCATTTGTGA AAGTGGACTC ACACTTTCGA
    CAGGGAATTC CCTTCTTTGG GCAGGTGCGC CTAGTAGATG GGAAAGGCGT CCCTATACCA
    AATAAAGTCA TATTCATCAG AGGAAATGAA GCAAACTATT ACTCCAATGC TACCACGGAT
    GAGCATGGCC TTGTACAGTT CTCTATCAAC ACCACCAATG TTATGGGTAC CTCTCTTACT
    GTTAGGGTCA ATTACAAGGA TCGTAGTCCC TGTTACGGCT ACCAGTGGGT GTCAGAAGAA
    CACGAAGAGG CACATCACAC TGCTTATCTT GTGTTCTCCC CAAGCAAGAG CTTTGTCCAC
    CTTGAGCCCA TGTCTCATGA ACTACCCTGT GGCCATACTC AGACAGTCCA GGCACATTAT
    ATTCTGAATG GAGGCACCCT GCTGGGGCTG AAGAAGCTCT CCTTCTATTA TCTGATAATG
    GCAAAGGGAG GCATTGTCCG AACTGGGACT CATGGACTGC TTGTGAAGCA GGAAGACATG
    AAGGGCCATT TTTCCATCTC AATCCCTGTG AAGTCAGACA TTGCTCCTGT CGCTCGGTTG
    CTCATCTATG CTGTTTTACC TACCGGGGAC GTGATTGGGG ATTCTGCAAA ATATGATGTT
    GAAAATTGTC TGGCCAACAA GGTGGATTTG AGCTTCAGCC CATCACAAAG TCTCCCAGCC
    TCACACGCCC ACCTGCGAGT CACAGCGGCT CCTCAGTCCG TCTGCGCCCT CCGTGCTGTG
    GACCAAAGCG TGCTGCTCAT GAAGCCTGAT GCTGAGCTCT CGGCGTCCTC GGTTTACAAC
    CTGCTACCAG AAAAGGACCT CACTGGCTTC CCTGGGCCTT TGAATGACCA GGACAATGAA
    GACTGCATCA ATCGTCATAA TGTCTATATT AATGGAATCA CATATACTCC AGTATCAAGT
    ACAAATGAAA AGGATATGTA CAGCTTCCTA GAGGACATGG GCTTAAAGGC ATTCACCAAC
    TCAAAGATTC GTAAACCCAA AATGTGTCCA CAGCTTCAAC AGTATGAAAT GCATGGACCT
    GAAGGTCTAC GTGTAGGTTT TTATGAGTCA GATGTAATGG GAAGAGGCCA TGCACGCCTG
    GTGCATGTTG AAGAGCCTCA CACGGAGACC GTACGAAAGT ACTTCCCTGA GACATGGATC
    TGGGATTTGG TGGTGGTAAA CTCAGCAGGT GTGGCTGAGG TAGGAGTAAC AGTCCCTGAC
    ACCATCACCG AGTGGAAGGC AGGGGCCTTC TGCCTGTCTG AAGATGCTGG ACTTGGTATC
    TCTTCCACTG CCTCTCTCCG AGCCTTCCAG CCCTTCTTTG TGGAGCTCAC AATGCCTTAC
    TCTGTGATTC GTGGAGAGGC CTTCACACTC AAGGCCACGG TCCTAAACTA CCTTCCCAAA
    TGCATCCGGG TCAGTGTGCA GCTGGAAGCC TCTCCCGCCT TCCTAGCTGT CCCAGTGGAG
    AAGGAACAAG CGCCTCACTG CATCTGTGCA AACGGGCGGC AAACTGTGTC CTGGGCAGTA
    ACCCCAAAGT CATTAGGAAA TGTGAATTTC ACTGTGAGCG CAGAGGCACT AGAGTCTCAA
    GAGCTGTGTG GGACTGAGGT GCCTTCAGTT CCTGAACACG GAAGGAAAGA CACAGTCATC
    AAGCCTCTGT TGGTTGAACC TGAAGGACTA GAGAAGGAAA CAACATTCAA CTCCCTACTT
    TGTCCATCAG GTGGTGAGGT TTCTGAAGAA TTATCCCTGA AACTGCCACC AAATGTGGTA
    GAAGAATCTG CCCGAGCTTC TGTCTCAGTT TTGGGAGACA TATTAGGCTC TGCCATGCAA
    AACACACAAA ATCTTCTCCA GATGCCCTAT GGCTGTGGAG AGCAGAATAT GGTCCTCTTT
    GCTCCTAACA TCTATGTACT GGATTATCTA AATGAAACAC AGCAGCTTAC TCCAGAGATC
    AAGTCCAAGG CCATTGGCTA TCTCAACACT GGTTACCAGA GACAGTTGAA CTACAAACAC
    TATGATGGCT CCTACAGCAC CTTTGGGGAG CGATATGGCA GGAACCAGGG CAACACCTGG
    CTCACAGCCT TTGTTCTGAA GACTTTTGCC CAAGCTCGAG CCTACATCTT CATCGATGAA
    GCACACATTA CCCAAGCCCT CATATGGCTC TCCCAGAGGC AGAAGGACAA TGGCTGTTTC
    AGGAGCTCTG GGTCACTGCT CAACAATGCC ATAAAGGGAG GAGTAGAAGA TGAAGTGACC
    CTCTCCGCCT ATATCACCAT CGCCCTTCTG GAGATTCCTC TCACAGTCAC TCACCCTGTT
    GTCCGCAATG CCCTGTTTTG CCTGGAGTCA GCCTGGAAGA CAGCACAAGA AGGGGACCAT
    GGCAGCCATG TATATACCAA AGCACTGCTG GCCTATGCTT TTGCCCTGGC AGGTAACCAG
    GACAAGAGGA AGGAAGTACT CAAGTCACTT AATGAGGAAG CTGTGAAGAA AGACAACTCT
    GTCCATTGGG AGCGCCCTCA GAAACCCAAG GCACCAGTGG GGCATTTTTA CGAACCCCAG
    GCTCCCTCTG CTGAGGTGGA GATGACATCC TATGTGCTCC TCGCTTATCT CACGGCCCAG
    CCAGCCCCAA CCTCGGAGGA CCTGACCTCT GCAACCAACA TCGTGAAGTG GATCACGAAG
    CAGCAGAATG CCCAGGGCGG TTTCTCCTCC ACCCAGGACA CAGTGGTGGC TCTCCATGCT
    CTGTCCAAAT ATGGAGCAGC CACATTTACC AGGACTGGGA AGGCTGCACA GGTGACTATC
    CAGTCTTCAG GGACATTTTC CAGCAAATTC CAAGTGGACA ACAACAACCG CCTGTTACTG
    CAGCAGGTCT CATTGCCAGA GCTGCCTGGG GAATACAGCA TGAAAGTGAC AGGAGAAGGA
    TGTGTCTACC TCCAGACATC CTTGAAATAC AATATTCTCC CAGAAAAGGA AGAGTTCCCC
    TTTGCTTTAG GAGTGCAGAC TCTGCCTCAA ACTTGTGATG AACCCAAAGC CCACACCAGC
    TTCCAAATCT CCCTAAGTGT CAGTTACACA GGGAGCCGCT CTGCCTCCAA CATGGCGATC
    GTTGATGTGA AGATGGTCTC TGGCTTCATT CCCCTGAAGC CAACAGTGAA AATGCTTGAA
    AGATCTAACC ATGTGAGCCG GACAGAAGTC AGCAGCAACC ATGTCTTGAT TTACCTTGAT
    AAGGTGTCAA ATCAGACACT GAGCTTGTTC TTCACGGTTC TGCAAGATGT CCCAGTAAGA
    GATCTGAAAC CAGCCATAGT GAAAGTCTAT GATTACTACG AGACGGATGA GTTTGCAATT
    GCTGAGTACA ATGCTCCTTG CAGCAAAGAT CTTGGAAATG CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu109275
    Clone ID Related Accession (Same CDS sequence) NM_001347425.2
    Accession Version NM_001347425.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-29
    Organism Homo sapiens(human)
    Product alpha-2-macroglobulin isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC007436.1. On Nov 30, 2019 this sequence version replaced NM_001347425.1. Transcript Variant: This variant (4) lacks an exon in the 5' region, and it thus differs in its 5' UTR and initiates translation from a downstream in-frame start codon, compared to variant 1. The encoded isoform (c) is shorter at the N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK307832.1, SRR1660807.96034.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGAAA ACTTTCACCC CCTGAATGAG TTGATTCCAC TAGTATACAT TCAGGATCCC 
    AAAGGAAATC GCATCGCACA ATGGCAGAGT TTCCAGTTAG AGGGTGGCCT CAAGCAATTT
    TCTTTTCCCC TCTCATCAGA GCCCTTCCAG GGCTCCTACA AGGTGGTGGT ACAGAAGAAA
    TCAGGTGGAA GGACAGAGCA CCCTTTCACC GTGGAGGAAT TTGTTCTTCC CAAGTTTGAA
    GTACAAGTAA CAGTGCCAAA GATAATCACC ATCTTGGAAG AAGAGATGAA TGTATCAGTG
    TGTGGCCTAT ACACATATGG GAAGCCTGTC CCTGGACATG TGACTGTGAG CATTTGCAGA
    AAGTATAGTG ACGCTTCCGA CTGCCACGGT GAAGATTCAC AGGCTTTCTG TGAGAAATTC
    AGTGGACAGC TAAACAGCCA TGGCTGCTTC TATCAGCAAG TAAAAACCAA GGTCTTCCAG
    CTGAAGAGGA AGGAGTATGA AATGAAACTT CACACTGAGG CCCAGATCCA AGAAGAAGGA
    ACAGTGGTGG AATTGACTGG AAGGCAGTCC AGTGAAATCA CAAGAACCAT AACCAAACTC
    TCATTTGTGA AAGTGGACTC ACACTTTCGA CAGGGAATTC CCTTCTTTGG GCAGGTGCGC
    CTAGTAGATG GGAAAGGCGT CCCTATACCA AATAAAGTCA TATTCATCAG AGGAAATGAA
    GCAAACTATT ACTCCAATGC TACCACGGAT GAGCATGGCC TTGTACAGTT CTCTATCAAC
    ACCACCAATG TTATGGGTAC CTCTCTTACT GTTAGGGTCA ATTACAAGGA TCGTAGTCCC
    TGTTACGGCT ACCAGTGGGT GTCAGAAGAA CACGAAGAGG CACATCACAC TGCTTATCTT
    GTGTTCTCCC CAAGCAAGAG CTTTGTCCAC CTTGAGCCCA TGTCTCATGA ACTACCCTGT
    GGCCATACTC AGACAGTCCA GGCACATTAT ATTCTGAATG GAGGCACCCT GCTGGGGCTG
    AAGAAGCTCT CCTTCTATTA TCTGATAATG GCAAAGGGAG GCATTGTCCG AACTGGGACT
    CATGGACTGC TTGTGAAGCA GGAAGACATG AAGGGCCATT TTTCCATCTC AATCCCTGTG
    AAGTCAGACA TTGCTCCTGT CGCTCGGTTG CTCATCTATG CTGTTTTACC TACCGGGGAC
    GTGATTGGGG ATTCTGCAAA ATATGATGTT GAAAATTGTC TGGCCAACAA GGTGGATTTG
    AGCTTCAGCC CATCACAAAG TCTCCCAGCC TCACACGCCC ACCTGCGAGT CACAGCGGCT
    CCTCAGTCCG TCTGCGCCCT CCGTGCTGTG GACCAAAGCG TGCTGCTCAT GAAGCCTGAT
    GCTGAGCTCT CGGCGTCCTC GGTTTACAAC CTGCTACCAG AAAAGGACCT CACTGGCTTC
    CCTGGGCCTT TGAATGACCA GGACAATGAA GACTGCATCA ATCGTCATAA TGTCTATATT
    AATGGAATCA CATATACTCC AGTATCAAGT ACAAATGAAA AGGATATGTA CAGCTTCCTA
    GAGGACATGG GCTTAAAGGC ATTCACCAAC TCAAAGATTC GTAAACCCAA AATGTGTCCA
    CAGCTTCAAC AGTATGAAAT GCATGGACCT GAAGGTCTAC GTGTAGGTTT TTATGAGTCA
    GATGTAATGG GAAGAGGCCA TGCACGCCTG GTGCATGTTG AAGAGCCTCA CACGGAGACC
    GTACGAAAGT ACTTCCCTGA GACATGGATC TGGGATTTGG TGGTGGTAAA CTCAGCAGGT
    GTGGCTGAGG TAGGAGTAAC AGTCCCTGAC ACCATCACCG AGTGGAAGGC AGGGGCCTTC
    TGCCTGTCTG AAGATGCTGG ACTTGGTATC TCTTCCACTG CCTCTCTCCG AGCCTTCCAG
    CCCTTCTTTG TGGAGCTCAC AATGCCTTAC TCTGTGATTC GTGGAGAGGC CTTCACACTC
    AAGGCCACGG TCCTAAACTA CCTTCCCAAA TGCATCCGGG TCAGTGTGCA GCTGGAAGCC
    TCTCCCGCCT TCCTAGCTGT CCCAGTGGAG AAGGAACAAG CGCCTCACTG CATCTGTGCA
    AACGGGCGGC AAACTGTGTC CTGGGCAGTA ACCCCAAAGT CATTAGGAAA TGTGAATTTC
    ACTGTGAGCG CAGAGGCACT AGAGTCTCAA GAGCTGTGTG GGACTGAGGT GCCTTCAGTT
    CCTGAACACG GAAGGAAAGA CACAGTCATC AAGCCTCTGT TGGTTGAACC TGAAGGACTA
    GAGAAGGAAA CAACATTCAA CTCCCTACTT TGTCCATCAG GTGGTGAGGT TTCTGAAGAA
    TTATCCCTGA AACTGCCACC AAATGTGGTA GAAGAATCTG CCCGAGCTTC TGTCTCAGTT
    TTGGGAGACA TATTAGGCTC TGCCATGCAA AACACACAAA ATCTTCTCCA GATGCCCTAT
    GGCTGTGGAG AGCAGAATAT GGTCCTCTTT GCTCCTAACA TCTATGTACT GGATTATCTA
    AATGAAACAC AGCAGCTTAC TCCAGAGATC AAGTCCAAGG CCATTGGCTA TCTCAACACT
    GGTTACCAGA GACAGTTGAA CTACAAACAC TATGATGGCT CCTACAGCAC CTTTGGGGAG
    CGATATGGCA GGAACCAGGG CAACACCTGG CTCACAGCCT TTGTTCTGAA GACTTTTGCC
    CAAGCTCGAG CCTACATCTT CATCGATGAA GCACACATTA CCCAAGCCCT CATATGGCTC
    TCCCAGAGGC AGAAGGACAA TGGCTGTTTC AGGAGCTCTG GGTCACTGCT CAACAATGCC
    ATAAAGGGAG GAGTAGAAGA TGAAGTGACC CTCTCCGCCT ATATCACCAT CGCCCTTCTG
    GAGATTCCTC TCACAGTCAC TCACCCTGTT GTCCGCAATG CCCTGTTTTG CCTGGAGTCA
    GCCTGGAAGA CAGCACAAGA AGGGGACCAT GGCAGCCATG TATATACCAA AGCACTGCTG
    GCCTATGCTT TTGCCCTGGC AGGTAACCAG GACAAGAGGA AGGAAGTACT CAAGTCACTT
    AATGAGGAAG CTGTGAAGAA AGACAACTCT GTCCATTGGG AGCGCCCTCA GAAACCCAAG
    GCACCAGTGG GGCATTTTTA CGAACCCCAG GCTCCCTCTG CTGAGGTGGA GATGACATCC
    TATGTGCTCC TCGCTTATCT CACGGCCCAG CCAGCCCCAA CCTCGGAGGA CCTGACCTCT
    GCAACCAACA TCGTGAAGTG GATCACGAAG CAGCAGAATG CCCAGGGCGG TTTCTCCTCC
    ACCCAGGACA CAGTGGTGGC TCTCCATGCT CTGTCCAAAT ATGGAGCAGC CACATTTACC
    AGGACTGGGA AGGCTGCACA GGTGACTATC CAGTCTTCAG GGACATTTTC CAGCAAATTC
    CAAGTGGACA ACAACAACCG CCTGTTACTG CAGCAGGTCT CATTGCCAGA GCTGCCTGGG
    GAATACAGCA TGAAAGTGAC AGGAGAAGGA TGTGTCTACC TCCAGACATC CTTGAAATAC
    AATATTCTCC CAGAAAAGGA AGAGTTCCCC TTTGCTTTAG GAGTGCAGAC TCTGCCTCAA
    ACTTGTGATG AACCCAAAGC CCACACCAGC TTCCAAATCT CCCTAAGTGT CAGTTACACA
    GGGAGCCGCT CTGCCTCCAA CATGGCGATC GTTGATGTGA AGATGGTCTC TGGCTTCATT
    CCCCTGAAGC CAACAGTGAA AATGCTTGAA AGATCTAACC ATGTGAGCCG GACAGAAGTC
    AGCAGCAACC ATGTCTTGAT TTACCTTGAT AAGGTGTCAA ATCAGACACT GAGCTTGTTC
    TTCACGGTTC TGCAAGATGT CCCAGTAAGA GATCTGAAAC CAGCCATAGT GAAAGTCTAT
    GATTACTACG AGACGGATGA GTTTGCAATT GCTGAGTACA ATGCTCCTTG CAGCAAAGAT
    CTTGGAAATG CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001334354.2
    CDS361..4335
    Translation

    Target ORF information:

    RefSeq Version NM_001347425.2
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha-2-macroglobulin (A2M), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001347425.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGAAA ACTTTCACCC CCTGAATGAG TTGATTCCAC TAGTATACAT TCAGGATCCC 
    AAAGGAAATC GCATCGCACA ATGGCAGAGT TTCCAGTTAG AGGGTGGCCT CAAGCAATTT
    TCTTTTCCCC TCTCATCAGA GCCCTTCCAG GGCTCCTACA AGGTGGTGGT ACAGAAGAAA
    TCAGGTGGAA GGACAGAGCA CCCTTTCACC GTGGAGGAAT TTGTTCTTCC CAAGTTTGAA
    GTACAAGTAA CAGTGCCAAA GATAATCACC ATCTTGGAAG AAGAGATGAA TGTATCAGTG
    TGTGGCCTAT ACACATATGG GAAGCCTGTC CCTGGACATG TGACTGTGAG CATTTGCAGA
    AAGTATAGTG ACGCTTCCGA CTGCCACGGT GAAGATTCAC AGGCTTTCTG TGAGAAATTC
    AGTGGACAGC TAAACAGCCA TGGCTGCTTC TATCAGCAAG TAAAAACCAA GGTCTTCCAG
    CTGAAGAGGA AGGAGTATGA AATGAAACTT CACACTGAGG CCCAGATCCA AGAAGAAGGA
    ACAGTGGTGG AATTGACTGG AAGGCAGTCC AGTGAAATCA CAAGAACCAT AACCAAACTC
    TCATTTGTGA AAGTGGACTC ACACTTTCGA CAGGGAATTC CCTTCTTTGG GCAGGTGCGC
    CTAGTAGATG GGAAAGGCGT CCCTATACCA AATAAAGTCA TATTCATCAG AGGAAATGAA
    GCAAACTATT ACTCCAATGC TACCACGGAT GAGCATGGCC TTGTACAGTT CTCTATCAAC
    ACCACCAATG TTATGGGTAC CTCTCTTACT GTTAGGGTCA ATTACAAGGA TCGTAGTCCC
    TGTTACGGCT ACCAGTGGGT GTCAGAAGAA CACGAAGAGG CACATCACAC TGCTTATCTT
    GTGTTCTCCC CAAGCAAGAG CTTTGTCCAC CTTGAGCCCA TGTCTCATGA ACTACCCTGT
    GGCCATACTC AGACAGTCCA GGCACATTAT ATTCTGAATG GAGGCACCCT GCTGGGGCTG
    AAGAAGCTCT CCTTCTATTA TCTGATAATG GCAAAGGGAG GCATTGTCCG AACTGGGACT
    CATGGACTGC TTGTGAAGCA GGAAGACATG AAGGGCCATT TTTCCATCTC AATCCCTGTG
    AAGTCAGACA TTGCTCCTGT CGCTCGGTTG CTCATCTATG CTGTTTTACC TACCGGGGAC
    GTGATTGGGG ATTCTGCAAA ATATGATGTT GAAAATTGTC TGGCCAACAA GGTGGATTTG
    AGCTTCAGCC CATCACAAAG TCTCCCAGCC TCACACGCCC ACCTGCGAGT CACAGCGGCT
    CCTCAGTCCG TCTGCGCCCT CCGTGCTGTG GACCAAAGCG TGCTGCTCAT GAAGCCTGAT
    GCTGAGCTCT CGGCGTCCTC GGTTTACAAC CTGCTACCAG AAAAGGACCT CACTGGCTTC
    CCTGGGCCTT TGAATGACCA GGACAATGAA GACTGCATCA ATCGTCATAA TGTCTATATT
    AATGGAATCA CATATACTCC AGTATCAAGT ACAAATGAAA AGGATATGTA CAGCTTCCTA
    GAGGACATGG GCTTAAAGGC ATTCACCAAC TCAAAGATTC GTAAACCCAA AATGTGTCCA
    CAGCTTCAAC AGTATGAAAT GCATGGACCT GAAGGTCTAC GTGTAGGTTT TTATGAGTCA
    GATGTAATGG GAAGAGGCCA TGCACGCCTG GTGCATGTTG AAGAGCCTCA CACGGAGACC
    GTACGAAAGT ACTTCCCTGA GACATGGATC TGGGATTTGG TGGTGGTAAA CTCAGCAGGT
    GTGGCTGAGG TAGGAGTAAC AGTCCCTGAC ACCATCACCG AGTGGAAGGC AGGGGCCTTC
    TGCCTGTCTG AAGATGCTGG ACTTGGTATC TCTTCCACTG CCTCTCTCCG AGCCTTCCAG
    CCCTTCTTTG TGGAGCTCAC AATGCCTTAC TCTGTGATTC GTGGAGAGGC CTTCACACTC
    AAGGCCACGG TCCTAAACTA CCTTCCCAAA TGCATCCGGG TCAGTGTGCA GCTGGAAGCC
    TCTCCCGCCT TCCTAGCTGT CCCAGTGGAG AAGGAACAAG CGCCTCACTG CATCTGTGCA
    AACGGGCGGC AAACTGTGTC CTGGGCAGTA ACCCCAAAGT CATTAGGAAA TGTGAATTTC
    ACTGTGAGCG CAGAGGCACT AGAGTCTCAA GAGCTGTGTG GGACTGAGGT GCCTTCAGTT
    CCTGAACACG GAAGGAAAGA CACAGTCATC AAGCCTCTGT TGGTTGAACC TGAAGGACTA
    GAGAAGGAAA CAACATTCAA CTCCCTACTT TGTCCATCAG GTGGTGAGGT TTCTGAAGAA
    TTATCCCTGA AACTGCCACC AAATGTGGTA GAAGAATCTG CCCGAGCTTC TGTCTCAGTT
    TTGGGAGACA TATTAGGCTC TGCCATGCAA AACACACAAA ATCTTCTCCA GATGCCCTAT
    GGCTGTGGAG AGCAGAATAT GGTCCTCTTT GCTCCTAACA TCTATGTACT GGATTATCTA
    AATGAAACAC AGCAGCTTAC TCCAGAGATC AAGTCCAAGG CCATTGGCTA TCTCAACACT
    GGTTACCAGA GACAGTTGAA CTACAAACAC TATGATGGCT CCTACAGCAC CTTTGGGGAG
    CGATATGGCA GGAACCAGGG CAACACCTGG CTCACAGCCT TTGTTCTGAA GACTTTTGCC
    CAAGCTCGAG CCTACATCTT CATCGATGAA GCACACATTA CCCAAGCCCT CATATGGCTC
    TCCCAGAGGC AGAAGGACAA TGGCTGTTTC AGGAGCTCTG GGTCACTGCT CAACAATGCC
    ATAAAGGGAG GAGTAGAAGA TGAAGTGACC CTCTCCGCCT ATATCACCAT CGCCCTTCTG
    GAGATTCCTC TCACAGTCAC TCACCCTGTT GTCCGCAATG CCCTGTTTTG CCTGGAGTCA
    GCCTGGAAGA CAGCACAAGA AGGGGACCAT GGCAGCCATG TATATACCAA AGCACTGCTG
    GCCTATGCTT TTGCCCTGGC AGGTAACCAG GACAAGAGGA AGGAAGTACT CAAGTCACTT
    AATGAGGAAG CTGTGAAGAA AGACAACTCT GTCCATTGGG AGCGCCCTCA GAAACCCAAG
    GCACCAGTGG GGCATTTTTA CGAACCCCAG GCTCCCTCTG CTGAGGTGGA GATGACATCC
    TATGTGCTCC TCGCTTATCT CACGGCCCAG CCAGCCCCAA CCTCGGAGGA CCTGACCTCT
    GCAACCAACA TCGTGAAGTG GATCACGAAG CAGCAGAATG CCCAGGGCGG TTTCTCCTCC
    ACCCAGGACA CAGTGGTGGC TCTCCATGCT CTGTCCAAAT ATGGAGCAGC CACATTTACC
    AGGACTGGGA AGGCTGCACA GGTGACTATC CAGTCTTCAG GGACATTTTC CAGCAAATTC
    CAAGTGGACA ACAACAACCG CCTGTTACTG CAGCAGGTCT CATTGCCAGA GCTGCCTGGG
    GAATACAGCA TGAAAGTGAC AGGAGAAGGA TGTGTCTACC TCCAGACATC CTTGAAATAC
    AATATTCTCC CAGAAAAGGA AGAGTTCCCC TTTGCTTTAG GAGTGCAGAC TCTGCCTCAA
    ACTTGTGATG AACCCAAAGC CCACACCAGC TTCCAAATCT CCCTAAGTGT CAGTTACACA
    GGGAGCCGCT CTGCCTCCAA CATGGCGATC GTTGATGTGA AGATGGTCTC TGGCTTCATT
    CCCCTGAAGC CAACAGTGAA AATGCTTGAA AGATCTAACC ATGTGAGCCG GACAGAAGTC
    AGCAGCAACC ATGTCTTGAT TTACCTTGAT AAGGTGTCAA ATCAGACACT GAGCTTGTTC
    TTCACGGTTC TGCAAGATGT CCCAGTAAGA GATCTGAAAC CAGCCATAGT GAAAGTCTAT
    GATTACTACG AGACGGATGA GTTTGCAATT GCTGAGTACA ATGCTCCTTG CAGCAAAGAT
    CTTGGAAATG CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18488
    Clone ID Related Accession (Same CDS sequence) NM_001347423.1 , NM_000014.5
    Accession Version NM_001347423.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4425bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-24
    Organism Homo sapiens(human)
    Product alpha-2-macroglobulin isoform a precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC334781.1, BC040071.1 and AB209614.2. Transcript Variant: This variant (2) splices out a region of the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.140912.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGGGAAGA ACAAACTCCT TCATCCAAGT CTGGTTCTTC TCCTCTTGGT CCTCCTGCCC 
    ACAGACGCCT CAGTCTCTGG AAAACCGCAG TATATGGTTC TGGTCCCCTC CCTGCTCCAC
    ACTGAGACCA CTGAGAAGGG CTGTGTCCTT CTGAGCTACC TGAATGAGAC AGTGACTGTA
    AGTGCTTCCT TGGAGTCTGT CAGGGGAAAC AGGAGCCTCT TCACTGACCT GGAGGCGGAG
    AATGACGTAC TCCACTGTGT CGCCTTCGCT GTCCCAAAGT CTTCATCCAA TGAGGAGGTA
    ATGTTCCTCA CTGTCCAAGT GAAAGGACCA ACCCAAGAAT TTAAGAAGCG GACCACAGTG
    ATGGTTAAGA ACGAGGACAG TCTGGTCTTT GTCCAGACAG ACAAATCAAT CTACAAACCA
    GGGCAGACAG TGAAATTTCG TGTTGTCTCC ATGGATGAAA ACTTTCACCC CCTGAATGAG
    TTGATTCCAC TAGTATACAT TCAGGATCCC AAAGGAAATC GCATCGCACA ATGGCAGAGT
    TTCCAGTTAG AGGGTGGCCT CAAGCAATTT TCTTTTCCCC TCTCATCAGA GCCCTTCCAG
    GGCTCCTACA AGGTGGTGGT ACAGAAGAAA TCAGGTGGAA GGACAGAGCA CCCTTTCACC
    GTGGAGGAAT TTGTTCTTCC CAAGTTTGAA GTACAAGTAA CAGTGCCAAA GATAATCACC
    ATCTTGGAAG AAGAGATGAA TGTATCAGTG TGTGGCCTAT ACACATATGG GAAGCCTGTC
    CCTGGACATG TGACTGTGAG CATTTGCAGA AAGTATAGTG ACGCTTCCGA CTGCCACGGT
    GAAGATTCAC AGGCTTTCTG TGAGAAATTC AGTGGACAGC TAAACAGCCA TGGCTGCTTC
    TATCAGCAAG TAAAAACCAA GGTCTTCCAG CTGAAGAGGA AGGAGTATGA AATGAAACTT
    CACACTGAGG CCCAGATCCA AGAAGAAGGA ACAGTGGTGG AATTGACTGG AAGGCAGTCC
    AGTGAAATCA CAAGAACCAT AACCAAACTC TCATTTGTGA AAGTGGACTC ACACTTTCGA
    CAGGGAATTC CCTTCTTTGG GCAGGTGCGC CTAGTAGATG GGAAAGGCGT CCCTATACCA
    AATAAAGTCA TATTCATCAG AGGAAATGAA GCAAACTATT ACTCCAATGC TACCACGGAT
    GAGCATGGCC TTGTACAGTT CTCTATCAAC ACCACCAATG TTATGGGTAC CTCTCTTACT
    GTTAGGGTCA ATTACAAGGA TCGTAGTCCC TGTTACGGCT ACCAGTGGGT GTCAGAAGAA
    CACGAAGAGG CACATCACAC TGCTTATCTT GTGTTCTCCC CAAGCAAGAG CTTTGTCCAC
    CTTGAGCCCA TGTCTCATGA ACTACCCTGT GGCCATACTC AGACAGTCCA GGCACATTAT
    ATTCTGAATG GAGGCACCCT GCTGGGGCTG AAGAAGCTCT CCTTCTATTA TCTGATAATG
    GCAAAGGGAG GCATTGTCCG AACTGGGACT CATGGACTGC TTGTGAAGCA GGAAGACATG
    AAGGGCCATT TTTCCATCTC AATCCCTGTG AAGTCAGACA TTGCTCCTGT CGCTCGGTTG
    CTCATCTATG CTGTTTTACC TACCGGGGAC GTGATTGGGG ATTCTGCAAA ATATGATGTT
    GAAAATTGTC TGGCCAACAA GGTGGATTTG AGCTTCAGCC CATCACAAAG TCTCCCAGCC
    TCACACGCCC ACCTGCGAGT CACAGCGGCT CCTCAGTCCG TCTGCGCCCT CCGTGCTGTG
    GACCAAAGCG TGCTGCTCAT GAAGCCTGAT GCTGAGCTCT CGGCGTCCTC GGTTTACAAC
    CTGCTACCAG AAAAGGACCT CACTGGCTTC CCTGGGCCTT TGAATGACCA GGACGATGAA
    GACTGCATCA ATCGTCATAA TGTCTATATT AATGGAATCA CATATACTCC AGTATCAAGT
    ACAAATGAAA AGGATATGTA CAGCTTCCTA GAGGACATGG GCTTAAAGGC ATTCACCAAC
    TCAAAGATTC GTAAACCCAA AATGTGTCCA CAGCTTCAAC AGTATGAAAT GCATGGACCT
    GAAGGTCTAC GTGTAGGTTT TTATGAGTCA GATGTAATGG GAAGAGGCCA TGCACGCCTG
    GTGCATGTTG AAGAGCCTCA CACGGAGACC GTACGAAAGT ACTTCCCTGA GACATGGATC
    TGGGATTTGG TGGTGGTAAA CTCAGCAGGT GTGGCTGAGG TAGGAGTAAC AGTCCCTGAC
    ACCATCACCG AGTGGAAGGC AGGGGCCTTC TGCCTGTCTG AAGATGCTGG ACTTGGTATC
    TCTTCCACTG CCTCTCTCCG AGCCTTCCAG CCCTTCTTTG TGGAGCTCAC AATGCCTTAC
    TCTGTGATTC GTGGAGAGGC CTTCACACTC AAGGCCACGG TCCTAAACTA CCTTCCCAAA
    TGCATCCGGG TCAGTGTGCA GCTGGAAGCC TCTCCCGCCT TCCTAGCTGT CCCAGTGGAG
    AAGGAACAAG CGCCTCACTG CATCTGTGCA AACGGGCGGC AAACTGTGTC CTGGGCAGTA
    ACCCCAAAGT CATTAGGAAA TGTGAATTTC ACTGTGAGCG CAGAGGCACT AGAGTCTCAA
    GAGCTGTGTG GGACTGAGGT GCCTTCAGTT CCTGAACACG GAAGGAAAGA CACAGTCATC
    AAGCCTCTGT TGGTTGAACC TGAAGGACTA GAGAAGGAAA CAACATTCAA CTCCCTACTT
    TGTCCATCAG GTGGTGAGGT TTCTGAAGAA TTATCCCTGA AACTGCCACC AAATGTGGTA
    GAAGAATCTG CCCGAGCTTC TGTCTCAGTT TTGGGAGACA TATTAGGCTC TGCCATGCAA
    AACACACAAA ATCTTCTCCA GATGCCCTAT GGCTGTGGAG AGCAGAATAT GGTCCTCTTT
    GCTCCTAACA TCTATGTACT GGATTATCTA AATGAAACAC AGCAGCTTAC TCCAGAGATC
    AAGTCCAAGG CCATTGGCTA TCTCAACACT GGTTACCAGA GACAGTTGAA CTACAAACAC
    TATGATGGCT CCTACAGCAC CTTTGGGGAG CGATATGGCA GGAACCAGGG CAACACCTGG
    CTCACAGCCT TTGTTCTGAA GACTTTTGCC CAAGCTCGAG CCTACATCTT CATCGATGAA
    GCACACATTA CCCAAGCCCT CATATGGCTC TCCCAGAGGC AGAAGGACAA TGGCTGTTTC
    AGGAGCTCTG GGTCACTGCT CAACAATGCC ATAAAGGGAG GAGTAGAAGA TGAAGTGACC
    CTCTCCGCCT ATATCACCAT CGCCCTTCTG GAGATTCCTC TCACAGTCAC TCACCCTGTT
    GTCCGCAATG CCCTGTTTTG CCTGGAGTCA GCCTGGAAGA CAGCACAAGA AGGGGACCAT
    GGCAGCCATG TATATACCAA AGCACTGCTG GCCTATGCTT TTGCCCTGGC AGGTAACCAG
    GACAAGAGGA AGGAAGTACT CAAGTCACTT AATGAGGAAG CTGTGAAGAA AGACAACTCT
    GTCCATTGGG AGCGCCCTCA GAAACCCAAG GCACCAGTGG GGCATTTTTA CGAACCCCAG
    GCTCCCTCTG CTGAGGTGGA GATGACATCC TATGTGCTCC TCGCTTATCT CACGGCCCAG
    CCAGCCCCAA CCTCGGAGGA CCTGACCTCT GCAACCAACA TCGTGAAGTG GATCACGAAG
    CAGCAGAATG CCCAGGGCGG TTTCTCCTCC ACCCAGGACA CAGTGGTGGC TCTCCATGCT
    CTGTCCAAAT ATGGAGCAGC CACATTTACC AGGACTGGGA AGGCTGCACA GGTGACTATC
    CAGTCTTCAG GGACATTTTC CAGCAAATTC CAAGTGGACA ACAACAACCG CCTGTTACTG
    CAGCAGGTCT CATTGCCAGA GCTGCCTGGG GAATACAGCA TGAAAGTGAC AGGAGAAGGA
    TGTGTCTACC TCCAGACATC CTTGAAATAC AATATTCTCC CAGAAAAGGA AGAGTTCCCC
    TTTGCTTTAG GAGTGCAGAC TCTGCCTCAA ACTTGTGATG AACCCAAAGC CCACACCAGC
    TTCCAAATCT CCCTAAGTGT CAGTTACACA GGGAGCCGCT CTGCCTCCAA CATGGCGATC
    GTTGATGTGA AGATGGTCTC TGGCTTCATT CCCCTGAAGC CAACAGTGAA AATGCTTGAA
    AGATCTAACC ATGTGAGCCG GACAGAAGTC AGCAGCAACC ATGTCTTGAT TTACCTTGAT
    AAGGTGTCAA ATCAGACACT GAGCTTGTTC TTCACGGTTC TGCAAGATGT CCCAGTAAGA
    GATCTGAAAC CAGCCATAGT GAAAGTCTAT GATTACTACG AGACGGATGA GTTTGCAATT
    GCTGAGTACA ATGCTCCTTG CAGCAAAGAT CTTGGAAATG CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001334352.1
    CDS120..4544
    Translation

    Target ORF information:

    RefSeq Version NM_001347423.1
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha-2-macroglobulin (A2M), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001347423.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGGGAAGA ACAAACTCCT TCATCCAAGT CTGGTTCTTC TCCTCTTGGT CCTCCTGCCC 
    ACAGACGCCT CAGTCTCTGG AAAACCGCAG TATATGGTTC TGGTCCCCTC CCTGCTCCAC
    ACTGAGACCA CTGAGAAGGG CTGTGTCCTT CTGAGCTACC TGAATGAGAC AGTGACTGTA
    AGTGCTTCCT TGGAGTCTGT CAGGGGAAAC AGGAGCCTCT TCACTGACCT GGAGGCGGAG
    AATGACGTAC TCCACTGTGT CGCCTTCGCT GTCCCAAAGT CTTCATCCAA TGAGGAGGTA
    ATGTTCCTCA CTGTCCAAGT GAAAGGACCA ACCCAAGAAT TTAAGAAGCG GACCACAGTG
    ATGGTTAAGA ACGAGGACAG TCTGGTCTTT GTCCAGACAG ACAAATCAAT CTACAAACCA
    GGGCAGACAG TGAAATTTCG TGTTGTCTCC ATGGATGAAA ACTTTCACCC CCTGAATGAG
    TTGATTCCAC TAGTATACAT TCAGGATCCC AAAGGAAATC GCATCGCACA ATGGCAGAGT
    TTCCAGTTAG AGGGTGGCCT CAAGCAATTT TCTTTTCCCC TCTCATCAGA GCCCTTCCAG
    GGCTCCTACA AGGTGGTGGT ACAGAAGAAA TCAGGTGGAA GGACAGAGCA CCCTTTCACC
    GTGGAGGAAT TTGTTCTTCC CAAGTTTGAA GTACAAGTAA CAGTGCCAAA GATAATCACC
    ATCTTGGAAG AAGAGATGAA TGTATCAGTG TGTGGCCTAT ACACATATGG GAAGCCTGTC
    CCTGGACATG TGACTGTGAG CATTTGCAGA AAGTATAGTG ACGCTTCCGA CTGCCACGGT
    GAAGATTCAC AGGCTTTCTG TGAGAAATTC AGTGGACAGC TAAACAGCCA TGGCTGCTTC
    TATCAGCAAG TAAAAACCAA GGTCTTCCAG CTGAAGAGGA AGGAGTATGA AATGAAACTT
    CACACTGAGG CCCAGATCCA AGAAGAAGGA ACAGTGGTGG AATTGACTGG AAGGCAGTCC
    AGTGAAATCA CAAGAACCAT AACCAAACTC TCATTTGTGA AAGTGGACTC ACACTTTCGA
    CAGGGAATTC CCTTCTTTGG GCAGGTGCGC CTAGTAGATG GGAAAGGCGT CCCTATACCA
    AATAAAGTCA TATTCATCAG AGGAAATGAA GCAAACTATT ACTCCAATGC TACCACGGAT
    GAGCATGGCC TTGTACAGTT CTCTATCAAC ACCACCAATG TTATGGGTAC CTCTCTTACT
    GTTAGGGTCA ATTACAAGGA TCGTAGTCCC TGTTACGGCT ACCAGTGGGT GTCAGAAGAA
    CACGAAGAGG CACATCACAC TGCTTATCTT GTGTTCTCCC CAAGCAAGAG CTTTGTCCAC
    CTTGAGCCCA TGTCTCATGA ACTACCCTGT GGCCATACTC AGACAGTCCA GGCACATTAT
    ATTCTGAATG GAGGCACCCT GCTGGGGCTG AAGAAGCTCT CCTTCTATTA TCTGATAATG
    GCAAAGGGAG GCATTGTCCG AACTGGGACT CATGGACTGC TTGTGAAGCA GGAAGACATG
    AAGGGCCATT TTTCCATCTC AATCCCTGTG AAGTCAGACA TTGCTCCTGT CGCTCGGTTG
    CTCATCTATG CTGTTTTACC TACCGGGGAC GTGATTGGGG ATTCTGCAAA ATATGATGTT
    GAAAATTGTC TGGCCAACAA GGTGGATTTG AGCTTCAGCC CATCACAAAG TCTCCCAGCC
    TCACACGCCC ACCTGCGAGT CACAGCGGCT CCTCAGTCCG TCTGCGCCCT CCGTGCTGTG
    GACCAAAGCG TGCTGCTCAT GAAGCCTGAT GCTGAGCTCT CGGCGTCCTC GGTTTACAAC
    CTGCTACCAG AAAAGGACCT CACTGGCTTC CCTGGGCCTT TGAATGACCA GGACGATGAA
    GACTGCATCA ATCGTCATAA TGTCTATATT AATGGAATCA CATATACTCC AGTATCAAGT
    ACAAATGAAA AGGATATGTA CAGCTTCCTA GAGGACATGG GCTTAAAGGC ATTCACCAAC
    TCAAAGATTC GTAAACCCAA AATGTGTCCA CAGCTTCAAC AGTATGAAAT GCATGGACCT
    GAAGGTCTAC GTGTAGGTTT TTATGAGTCA GATGTAATGG GAAGAGGCCA TGCACGCCTG
    GTGCATGTTG AAGAGCCTCA CACGGAGACC GTACGAAAGT ACTTCCCTGA GACATGGATC
    TGGGATTTGG TGGTGGTAAA CTCAGCAGGT GTGGCTGAGG TAGGAGTAAC AGTCCCTGAC
    ACCATCACCG AGTGGAAGGC AGGGGCCTTC TGCCTGTCTG AAGATGCTGG ACTTGGTATC
    TCTTCCACTG CCTCTCTCCG AGCCTTCCAG CCCTTCTTTG TGGAGCTCAC AATGCCTTAC
    TCTGTGATTC GTGGAGAGGC CTTCACACTC AAGGCCACGG TCCTAAACTA CCTTCCCAAA
    TGCATCCGGG TCAGTGTGCA GCTGGAAGCC TCTCCCGCCT TCCTAGCTGT CCCAGTGGAG
    AAGGAACAAG CGCCTCACTG CATCTGTGCA AACGGGCGGC AAACTGTGTC CTGGGCAGTA
    ACCCCAAAGT CATTAGGAAA TGTGAATTTC ACTGTGAGCG CAGAGGCACT AGAGTCTCAA
    GAGCTGTGTG GGACTGAGGT GCCTTCAGTT CCTGAACACG GAAGGAAAGA CACAGTCATC
    AAGCCTCTGT TGGTTGAACC TGAAGGACTA GAGAAGGAAA CAACATTCAA CTCCCTACTT
    TGTCCATCAG GTGGTGAGGT TTCTGAAGAA TTATCCCTGA AACTGCCACC AAATGTGGTA
    GAAGAATCTG CCCGAGCTTC TGTCTCAGTT TTGGGAGACA TATTAGGCTC TGCCATGCAA
    AACACACAAA ATCTTCTCCA GATGCCCTAT GGCTGTGGAG AGCAGAATAT GGTCCTCTTT
    GCTCCTAACA TCTATGTACT GGATTATCTA AATGAAACAC AGCAGCTTAC TCCAGAGATC
    AAGTCCAAGG CCATTGGCTA TCTCAACACT GGTTACCAGA GACAGTTGAA CTACAAACAC
    TATGATGGCT CCTACAGCAC CTTTGGGGAG CGATATGGCA GGAACCAGGG CAACACCTGG
    CTCACAGCCT TTGTTCTGAA GACTTTTGCC CAAGCTCGAG CCTACATCTT CATCGATGAA
    GCACACATTA CCCAAGCCCT CATATGGCTC TCCCAGAGGC AGAAGGACAA TGGCTGTTTC
    AGGAGCTCTG GGTCACTGCT CAACAATGCC ATAAAGGGAG GAGTAGAAGA TGAAGTGACC
    CTCTCCGCCT ATATCACCAT CGCCCTTCTG GAGATTCCTC TCACAGTCAC TCACCCTGTT
    GTCCGCAATG CCCTGTTTTG CCTGGAGTCA GCCTGGAAGA CAGCACAAGA AGGGGACCAT
    GGCAGCCATG TATATACCAA AGCACTGCTG GCCTATGCTT TTGCCCTGGC AGGTAACCAG
    GACAAGAGGA AGGAAGTACT CAAGTCACTT AATGAGGAAG CTGTGAAGAA AGACAACTCT
    GTCCATTGGG AGCGCCCTCA GAAACCCAAG GCACCAGTGG GGCATTTTTA CGAACCCCAG
    GCTCCCTCTG CTGAGGTGGA GATGACATCC TATGTGCTCC TCGCTTATCT CACGGCCCAG
    CCAGCCCCAA CCTCGGAGGA CCTGACCTCT GCAACCAACA TCGTGAAGTG GATCACGAAG
    CAGCAGAATG CCCAGGGCGG TTTCTCCTCC ACCCAGGACA CAGTGGTGGC TCTCCATGCT
    CTGTCCAAAT ATGGAGCAGC CACATTTACC AGGACTGGGA AGGCTGCACA GGTGACTATC
    CAGTCTTCAG GGACATTTTC CAGCAAATTC CAAGTGGACA ACAACAACCG CCTGTTACTG
    CAGCAGGTCT CATTGCCAGA GCTGCCTGGG GAATACAGCA TGAAAGTGAC AGGAGAAGGA
    TGTGTCTACC TCCAGACATC CTTGAAATAC AATATTCTCC CAGAAAAGGA AGAGTTCCCC
    TTTGCTTTAG GAGTGCAGAC TCTGCCTCAA ACTTGTGATG AACCCAAAGC CCACACCAGC
    TTCCAAATCT CCCTAAGTGT CAGTTACACA GGGAGCCGCT CTGCCTCCAA CATGGCGATC
    GTTGATGTGA AGATGGTCTC TGGCTTCATT CCCCTGAAGC CAACAGTGAA AATGCTTGAA
    AGATCTAACC ATGTGAGCCG GACAGAAGTC AGCAGCAACC ATGTCTTGAT TTACCTTGAT
    AAGGTGTCAA ATCAGACACT GAGCTTGTTC TTCACGGTTC TGCAAGATGT CCCAGTAAGA
    GATCTGAAAC CAGCCATAGT GAAAGTCTAT GATTACTACG AGACGGATGA GTTTGCAATT
    GCTGAGTACA ATGCTCCTTG CAGCAAAGAT CTTGGAAATG CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu46081
    Clone ID Related Accession (Same CDS sequence) XM_006719056.3 , XM_006719056.2
    Accession Version XM_006719056.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4539bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product alpha-2-macroglobulin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006719056.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGGAAGA ACAAACTCCT TCATCCAAGT CTGGTTCTTC TCCTCTTGGT CCTCCTGCCC 
    ACAGACGCCT CAGTCTCTGG AAAACCGCAG TATATGGTTC TGGTCCCCTC CCTGCTCCAC
    ACTGAGACCA CTGAGAAGGG CTGTGTCCTT CTGAGCTACC TGAATGAGAC AGTGACTGTA
    AGTGCTTCCT TGGAGTCTGT CAGGGGAAAC AGGAGCCTCT TCACTGACCT GGAGGCGGAG
    AATGACGTAC TCCACTGTGT CGCCTTCGCT GTCCCAAAGT CTTCATCCAA TGAGGAGGTA
    ATGTTCCTCA CTGTCCAAGT GAAAGGACCA ACCCAAGAAT TTAAGAAGCG GACCACAGTG
    ATGGTTAAGA ACGAGGACAG TCTGGTCTTT GTCCAGACAG ACAAATCAAT CTACAAACCA
    GGGCAGACAG TGAAATTTCG TGTTGTCTCC ATGGATGAAA ACTTTCACCC CCTGAATGAG
    TTGATTCCAC TAGTATACAT TCAGGATCCC AAAGGAAATC GCATCGCACA ATGGCAGAGT
    TTCCAGTTAG AGGGTGGCCT CAAGCAATTT TCTTTTCCCC TCTCATCAGA GCCCTTCCAG
    GGCTCCTACA AGGTGGTGGT ACAGAAGAAA TCAGGTGGAA GGACAGAGCA CCCTTTCACC
    GTGGAGGAAT TTGTTCTTCC CAAGTTTGAA GTACAAGTAA CAGTGCCAAA GATAATCACC
    ATCTTGGAAG AAGAGATGAA TGTATCAGTG TGTGGCCTAT ACACATATGG GAAGCCTGTC
    CCTGGACATG TGACTGTGAG CATTTGCAGA AAGTATAGTG ACGCTTCCGA CTGCCACGGT
    GAAGATTCAC AGGCTTTCTG TGAGAAATTC AGTGGACAGC TAAACAGCCA TGGCTGCTTC
    TATCAGCAAG TAAAAACCAA GGTCTTCCAG CTGAAGAGGA AGGAGTATGA AATGAAACTT
    CACACTGAGG CCCAGATCCA AGAAGAAGGA ACAGTGGTGG AATTGACTGG AAGGCAGTCC
    AGTGAAATCA CAAGAACCAT AACCAAACTC TCATTTGTGA AAGTGGACTC ACACTTTCGA
    CAGGGAATTC CCTTCTTTGG GCAGGTGCGC CTAGTAGATG GGAAAGGCGT CCCTATACCA
    AATAAAGTCA TATTCATCAG AGGAAATGAA GCAAACTATT ACTCCAATGC TACCACGGAT
    GAGCATGGCC TTGTACAGTT CTCTATCAAC ACCACCAATG TTATGGGTAC CTCTCTTACT
    GTTAGGGTCA ATTACAAGGA TCGTAGTCCC TGTTACGGCT ACCAGTGGGT GTCAGAAGAA
    CACGAAGAGG CACATCACAC TGCTTATCTT GTGTTCTCCC CAAGCAAGAG CTTTGTCCAC
    CTTGAGCCCA TGTCTCATGA ACTACCCTGT GGCCATACTC AGACAGTCCA GGCACATTAT
    ATTCTGAATG GAGGCACCCT GCTGGGGCTG AAGAAGCTCT CCTTCTATTA TCTGATAATG
    GCAAAGGGAG GCATTGTCCG AACTGGGACT CATGGACTGC TTGTGAAGCA GGAAGACATG
    AAGGGCCATT TTTCCATCTC AATCCCTGTG AAGTCAGACA TTGCTCCTGT CGCTCGGTTG
    CTCATCTATG CTGTTTTACC TACCGGGGAC GTGATTGGGG ATTCTGCAAA ATATGATGTT
    GAAAATTGTC TGGCCAACAA GGTGGATTTG AGCTTCAGCC CATCACAAAG TCTCCCAGCC
    TCACACGCCC ACCTGCGAGT CACAGCGGCT CCTCAGTCCG TCTGCGCCCT CCGTGCTGTG
    GACCAAAGCG TGCTGCTCAT GAAGCCTGAT GCTGAGCTCT CGGCGTCCTC GGTTTACAAC
    CTGCTACCAG AAAAGGACCT CACTGGCTTC CCTGGGCCTT TGAATGACCA GGACAATGAA
    GACTGCATCA ATCGTCATAA TGTCTATATT AATGGAATCA CATATACTCC AGTATCAAGT
    ACAAATGAAA AGGATATGTA CAGCTTCCTA GAGGACATGG GCTTAAAGGC ATTCACCAAC
    TCAAAGATTC GTAAACCCAA AATGTGTCCA CAGCTTCAAC AGTATGAAAT GCATGGACCT
    GAAGGTCTAC GTGTAGGTTT TTATGAGTCA GATGTAATGG GAAGAGGCCA TGCACGCCTG
    GTGCATGTTG AAGAGCCTCA CACGGAGACC GTACGAAAGT ACTTCCCTGA GACATGGATC
    TGGGATTTGG TGGTGGTAAA CTCAGCAGGT GTGGCTGAGG TAGGAGTAAC AGTCCCTGAC
    ACCATCACCG AGTGGAAGGC AGGGGCCTTC TGCCTGTCTG AAGATGCTGG ACTTGGTATC
    TCTTCCACTG CCTCTCTCCG AGCCTTCCAG CCCTTCTTTG TGGAGCTCAC AATGCCTTAC
    TCTGTGATTC GTGGAGAGGC CTTCACACTC AAGGCCACGG TCCTAAACTA CCTTCCCAAA
    TGCATCCGGG TCAGTGTGCA GCTGGAAGCC TCTCCCGCCT TCCTAGCTGT CCCAGTGGAG
    AAGGAACAAG CGCCTCACTG CATCTGTGCA AACGGGCGGC AAACTGTGTC CTGGGCAGTA
    ACCCCAAAGT CATTAGGAAA TGTGAATTTC ACTGTGAGCG CAGAGGCACT AGAGTCTCAA
    GAGCTGTGTG GGACTGAGGT GCCTTCAGTT CCTGAACACG GAAGGAAAGA CACAGTCATC
    AAGCCTCTGT TGGTTGAACC TGAAGGACTA GAGAAGGAAA CAACATTCAA CTCCCTACTT
    TGTCCATCAG GTGGTGAGGT TTCTGAAGAA TTATCCCTGA AACTGCCACC AAATGTGGTA
    GAAGAATCTG CCCGAGCTTC TGTCTCAGTT TTGGGAGACA TATTAGGCTC TGCCATGCAA
    AACACACAAA ATCTTCTCCA GATGCCCTAT GGCTGTGGAG AGCAGAATAT GGTCCTCTTT
    GCTCCTAACA TCTATGTACT GGATTATCTA AATGAAACAC AGCAGCTTAC TCCAGAGATC
    AAGTCCAAGG CCATTGGCTA TCTCAACACT GGTTACCAGA GACAGTTGAA CTACAAACAC
    TATGATGGCT CCTACAGCAC CTTTGGGGAG CGATATGGCA GGAACCAGGG CAACACCTGG
    CTCACAGCCT TTGTTCTGAA GACTTTTGCC CAAGCTCGAG CCTACATCTT CATCGATGAA
    GCACACATTA CCCAAGCCCT CATATGGCTC TCCCAGAGGC AGAAGGACAA TGGCTGTTTC
    AGGAGCTCTG GGTCACTGCT CAACAATGCC ATAAAGGGAG GAGTAGAAGA TGAAGTGACC
    CTCTCCGCCT ATATCACCAT CGCCCTTCTG GAGATTCCTC TCACAGTCAC TCACCCTGTT
    GTCCGCAATG CCCTGTTTTG CCTGGAGTCA GCCTGGAAGA CAGCACAAGA AGGGGACCAT
    GGCAGCCATG TATATACCAA AGCACTGCTG GCCTATGCTT TTGCCCTGGC AGGTAACCAG
    GACAAGAGGA AGGAAGTACT CAAGTCACTT AATGAGGAAG CTGTGAAGAA AGACAACTCT
    GTCCATTGGG AGCGCCCTCA GAAACCCAAG GCACCAGTGG GGCATTTTTA CGAACCCCAG
    GCTCCCTCTG CTGAGGTGGA GATGACATCC TATGTGCTCC TCGCTTATCT CACGGCCCAG
    CCAGCCCCAA CCTCGGAGGA CCTGACCTCT GCAACCAACA TCGTGAAGTG GATCACGAAG
    CAGCAGAATG CCCAGGGCGG TTTCTCCTCC ACCCAGGACA CAGTGGTGGC TCTCCATGCT
    CTGTCCAAAT ATGGAGCAGC CACATTTACC AGGACTGGGA AGGCTGCACA GGTGACTATC
    CAGTCTTCAG GGACATTTTC CAGCAAATTC CAAGTGGACA ACAACAACCG CCTGTTACTG
    CAGCAGGTCT CATTGCCAGA GCTGCCTGGG GAATACAGCA TGAAAGTGAC AGGAGAAGGA
    TGTGTCTACC TCCAGACATC CTTGAAATAC AATATTCTCC CAGAAAAGGA AGAGTTCCCC
    TTTGCTTTAG GAGTGCAGAC TCTGCCTCAA ACTTGTGATG AACCCAAAGC CCACACCAGC
    TTCCAAATCT CCCTAAGTGT CAGTTACACA GGGAGCCGCT CTGCCTCCAA CATGGCGATC
    GTTGATGTGA AGATGGTCTC TGGCTTCATT CCCCTGAAGC CAACAGTGAA AATGCTTGAA
    AGATCTAACC ATGTGAGCCG GACAGAAGTC AGCAGCAACC ATGTCTTGAT TTACCTTGAT
    AAGGTGTCAA ATCAGACACT GAGCTTGTTC TTCACGGTTC TGCAAGATGT CCCAGTAAGA
    GATCTGAAAC CAGCCATAGT GAAAGTCTAT GATTACTACG AGACGGATGA GTTTGCAATT
    GCTGAGTACA ATGCTCCTTG CAGCAAAGGT AAGCCACTCA CACTCCTCCA AAAGGCAGTC
    AGAGCTCCTT CAGCTTGCCC CCCAAACCTT CTCCTTCATA AAACGCTGGG TAAATATTTG
    TCAAAAACAT CAAATTACTC ACACTGCACA TTATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006719119.1
    CDS100..4638
    Translation

    Target ORF information:

    RefSeq Version XM_006719056.3
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha-2-macroglobulin (A2M), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006719056.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGGAAGA ACAAACTCCT TCATCCAAGT CTGGTTCTTC TCCTCTTGGT CCTCCTGCCC 
    ACAGACGCCT CAGTCTCTGG AAAACCGCAG TATATGGTTC TGGTCCCCTC CCTGCTCCAC
    ACTGAGACCA CTGAGAAGGG CTGTGTCCTT CTGAGCTACC TGAATGAGAC AGTGACTGTA
    AGTGCTTCCT TGGAGTCTGT CAGGGGAAAC AGGAGCCTCT TCACTGACCT GGAGGCGGAG
    AATGACGTAC TCCACTGTGT CGCCTTCGCT GTCCCAAAGT CTTCATCCAA TGAGGAGGTA
    ATGTTCCTCA CTGTCCAAGT GAAAGGACCA ACCCAAGAAT TTAAGAAGCG GACCACAGTG
    ATGGTTAAGA ACGAGGACAG TCTGGTCTTT GTCCAGACAG ACAAATCAAT CTACAAACCA
    GGGCAGACAG TGAAATTTCG TGTTGTCTCC ATGGATGAAA ACTTTCACCC CCTGAATGAG
    TTGATTCCAC TAGTATACAT TCAGGATCCC AAAGGAAATC GCATCGCACA ATGGCAGAGT
    TTCCAGTTAG AGGGTGGCCT CAAGCAATTT TCTTTTCCCC TCTCATCAGA GCCCTTCCAG
    GGCTCCTACA AGGTGGTGGT ACAGAAGAAA TCAGGTGGAA GGACAGAGCA CCCTTTCACC
    GTGGAGGAAT TTGTTCTTCC CAAGTTTGAA GTACAAGTAA CAGTGCCAAA GATAATCACC
    ATCTTGGAAG AAGAGATGAA TGTATCAGTG TGTGGCCTAT ACACATATGG GAAGCCTGTC
    CCTGGACATG TGACTGTGAG CATTTGCAGA AAGTATAGTG ACGCTTCCGA CTGCCACGGT
    GAAGATTCAC AGGCTTTCTG TGAGAAATTC AGTGGACAGC TAAACAGCCA TGGCTGCTTC
    TATCAGCAAG TAAAAACCAA GGTCTTCCAG CTGAAGAGGA AGGAGTATGA AATGAAACTT
    CACACTGAGG CCCAGATCCA AGAAGAAGGA ACAGTGGTGG AATTGACTGG AAGGCAGTCC
    AGTGAAATCA CAAGAACCAT AACCAAACTC TCATTTGTGA AAGTGGACTC ACACTTTCGA
    CAGGGAATTC CCTTCTTTGG GCAGGTGCGC CTAGTAGATG GGAAAGGCGT CCCTATACCA
    AATAAAGTCA TATTCATCAG AGGAAATGAA GCAAACTATT ACTCCAATGC TACCACGGAT
    GAGCATGGCC TTGTACAGTT CTCTATCAAC ACCACCAATG TTATGGGTAC CTCTCTTACT
    GTTAGGGTCA ATTACAAGGA TCGTAGTCCC TGTTACGGCT ACCAGTGGGT GTCAGAAGAA
    CACGAAGAGG CACATCACAC TGCTTATCTT GTGTTCTCCC CAAGCAAGAG CTTTGTCCAC
    CTTGAGCCCA TGTCTCATGA ACTACCCTGT GGCCATACTC AGACAGTCCA GGCACATTAT
    ATTCTGAATG GAGGCACCCT GCTGGGGCTG AAGAAGCTCT CCTTCTATTA TCTGATAATG
    GCAAAGGGAG GCATTGTCCG AACTGGGACT CATGGACTGC TTGTGAAGCA GGAAGACATG
    AAGGGCCATT TTTCCATCTC AATCCCTGTG AAGTCAGACA TTGCTCCTGT CGCTCGGTTG
    CTCATCTATG CTGTTTTACC TACCGGGGAC GTGATTGGGG ATTCTGCAAA ATATGATGTT
    GAAAATTGTC TGGCCAACAA GGTGGATTTG AGCTTCAGCC CATCACAAAG TCTCCCAGCC
    TCACACGCCC ACCTGCGAGT CACAGCGGCT CCTCAGTCCG TCTGCGCCCT CCGTGCTGTG
    GACCAAAGCG TGCTGCTCAT GAAGCCTGAT GCTGAGCTCT CGGCGTCCTC GGTTTACAAC
    CTGCTACCAG AAAAGGACCT CACTGGCTTC CCTGGGCCTT TGAATGACCA GGACAATGAA
    GACTGCATCA ATCGTCATAA TGTCTATATT AATGGAATCA CATATACTCC AGTATCAAGT
    ACAAATGAAA AGGATATGTA CAGCTTCCTA GAGGACATGG GCTTAAAGGC ATTCACCAAC
    TCAAAGATTC GTAAACCCAA AATGTGTCCA CAGCTTCAAC AGTATGAAAT GCATGGACCT
    GAAGGTCTAC GTGTAGGTTT TTATGAGTCA GATGTAATGG GAAGAGGCCA TGCACGCCTG
    GTGCATGTTG AAGAGCCTCA CACGGAGACC GTACGAAAGT ACTTCCCTGA GACATGGATC
    TGGGATTTGG TGGTGGTAAA CTCAGCAGGT GTGGCTGAGG TAGGAGTAAC AGTCCCTGAC
    ACCATCACCG AGTGGAAGGC AGGGGCCTTC TGCCTGTCTG AAGATGCTGG ACTTGGTATC
    TCTTCCACTG CCTCTCTCCG AGCCTTCCAG CCCTTCTTTG TGGAGCTCAC AATGCCTTAC
    TCTGTGATTC GTGGAGAGGC CTTCACACTC AAGGCCACGG TCCTAAACTA CCTTCCCAAA
    TGCATCCGGG TCAGTGTGCA GCTGGAAGCC TCTCCCGCCT TCCTAGCTGT CCCAGTGGAG
    AAGGAACAAG CGCCTCACTG CATCTGTGCA AACGGGCGGC AAACTGTGTC CTGGGCAGTA
    ACCCCAAAGT CATTAGGAAA TGTGAATTTC ACTGTGAGCG CAGAGGCACT AGAGTCTCAA
    GAGCTGTGTG GGACTGAGGT GCCTTCAGTT CCTGAACACG GAAGGAAAGA CACAGTCATC
    AAGCCTCTGT TGGTTGAACC TGAAGGACTA GAGAAGGAAA CAACATTCAA CTCCCTACTT
    TGTCCATCAG GTGGTGAGGT TTCTGAAGAA TTATCCCTGA AACTGCCACC AAATGTGGTA
    GAAGAATCTG CCCGAGCTTC TGTCTCAGTT TTGGGAGACA TATTAGGCTC TGCCATGCAA
    AACACACAAA ATCTTCTCCA GATGCCCTAT GGCTGTGGAG AGCAGAATAT GGTCCTCTTT
    GCTCCTAACA TCTATGTACT GGATTATCTA AATGAAACAC AGCAGCTTAC TCCAGAGATC
    AAGTCCAAGG CCATTGGCTA TCTCAACACT GGTTACCAGA GACAGTTGAA CTACAAACAC
    TATGATGGCT CCTACAGCAC CTTTGGGGAG CGATATGGCA GGAACCAGGG CAACACCTGG
    CTCACAGCCT TTGTTCTGAA GACTTTTGCC CAAGCTCGAG CCTACATCTT CATCGATGAA
    GCACACATTA CCCAAGCCCT CATATGGCTC TCCCAGAGGC AGAAGGACAA TGGCTGTTTC
    AGGAGCTCTG GGTCACTGCT CAACAATGCC ATAAAGGGAG GAGTAGAAGA TGAAGTGACC
    CTCTCCGCCT ATATCACCAT CGCCCTTCTG GAGATTCCTC TCACAGTCAC TCACCCTGTT
    GTCCGCAATG CCCTGTTTTG CCTGGAGTCA GCCTGGAAGA CAGCACAAGA AGGGGACCAT
    GGCAGCCATG TATATACCAA AGCACTGCTG GCCTATGCTT TTGCCCTGGC AGGTAACCAG
    GACAAGAGGA AGGAAGTACT CAAGTCACTT AATGAGGAAG CTGTGAAGAA AGACAACTCT
    GTCCATTGGG AGCGCCCTCA GAAACCCAAG GCACCAGTGG GGCATTTTTA CGAACCCCAG
    GCTCCCTCTG CTGAGGTGGA GATGACATCC TATGTGCTCC TCGCTTATCT CACGGCCCAG
    CCAGCCCCAA CCTCGGAGGA CCTGACCTCT GCAACCAACA TCGTGAAGTG GATCACGAAG
    CAGCAGAATG CCCAGGGCGG TTTCTCCTCC ACCCAGGACA CAGTGGTGGC TCTCCATGCT
    CTGTCCAAAT ATGGAGCAGC CACATTTACC AGGACTGGGA AGGCTGCACA GGTGACTATC
    CAGTCTTCAG GGACATTTTC CAGCAAATTC CAAGTGGACA ACAACAACCG CCTGTTACTG
    CAGCAGGTCT CATTGCCAGA GCTGCCTGGG GAATACAGCA TGAAAGTGAC AGGAGAAGGA
    TGTGTCTACC TCCAGACATC CTTGAAATAC AATATTCTCC CAGAAAAGGA AGAGTTCCCC
    TTTGCTTTAG GAGTGCAGAC TCTGCCTCAA ACTTGTGATG AACCCAAAGC CCACACCAGC
    TTCCAAATCT CCCTAAGTGT CAGTTACACA GGGAGCCGCT CTGCCTCCAA CATGGCGATC
    GTTGATGTGA AGATGGTCTC TGGCTTCATT CCCCTGAAGC CAACAGTGAA AATGCTTGAA
    AGATCTAACC ATGTGAGCCG GACAGAAGTC AGCAGCAACC ATGTCTTGAT TTACCTTGAT
    AAGGTGTCAA ATCAGACACT GAGCTTGTTC TTCACGGTTC TGCAAGATGT CCCAGTAAGA
    GATCTGAAAC CAGCCATAGT GAAAGTCTAT GATTACTACG AGACGGATGA GTTTGCAATT
    GCTGAGTACA ATGCTCCTTG CAGCAAAGGT AAGCCACTCA CACTCCTCCA AAAGGCAGTC
    AGAGCTCCTT CAGCTTGCCC CCCAAACCTT CTCCTTCATA AAACGCTGGG TAAATATTTG
    TCAAAAACAT CAAATTACTC ACACTGCACA TTATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu46081
    Clone ID Related Accession (Same CDS sequence) XM_006719056.3 , XM_006719056.2
    Accession Version XM_006719056.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4539bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product alpha-2-macroglobulin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006719056.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGGAAGA ACAAACTCCT TCATCCAAGT CTGGTTCTTC TCCTCTTGGT CCTCCTGCCC 
    ACAGACGCCT CAGTCTCTGG AAAACCGCAG TATATGGTTC TGGTCCCCTC CCTGCTCCAC
    ACTGAGACCA CTGAGAAGGG CTGTGTCCTT CTGAGCTACC TGAATGAGAC AGTGACTGTA
    AGTGCTTCCT TGGAGTCTGT CAGGGGAAAC AGGAGCCTCT TCACTGACCT GGAGGCGGAG
    AATGACGTAC TCCACTGTGT CGCCTTCGCT GTCCCAAAGT CTTCATCCAA TGAGGAGGTA
    ATGTTCCTCA CTGTCCAAGT GAAAGGACCA ACCCAAGAAT TTAAGAAGCG GACCACAGTG
    ATGGTTAAGA ACGAGGACAG TCTGGTCTTT GTCCAGACAG ACAAATCAAT CTACAAACCA
    GGGCAGACAG TGAAATTTCG TGTTGTCTCC ATGGATGAAA ACTTTCACCC CCTGAATGAG
    TTGATTCCAC TAGTATACAT TCAGGATCCC AAAGGAAATC GCATCGCACA ATGGCAGAGT
    TTCCAGTTAG AGGGTGGCCT CAAGCAATTT TCTTTTCCCC TCTCATCAGA GCCCTTCCAG
    GGCTCCTACA AGGTGGTGGT ACAGAAGAAA TCAGGTGGAA GGACAGAGCA CCCTTTCACC
    GTGGAGGAAT TTGTTCTTCC CAAGTTTGAA GTACAAGTAA CAGTGCCAAA GATAATCACC
    ATCTTGGAAG AAGAGATGAA TGTATCAGTG TGTGGCCTAT ACACATATGG GAAGCCTGTC
    CCTGGACATG TGACTGTGAG CATTTGCAGA AAGTATAGTG ACGCTTCCGA CTGCCACGGT
    GAAGATTCAC AGGCTTTCTG TGAGAAATTC AGTGGACAGC TAAACAGCCA TGGCTGCTTC
    TATCAGCAAG TAAAAACCAA GGTCTTCCAG CTGAAGAGGA AGGAGTATGA AATGAAACTT
    CACACTGAGG CCCAGATCCA AGAAGAAGGA ACAGTGGTGG AATTGACTGG AAGGCAGTCC
    AGTGAAATCA CAAGAACCAT AACCAAACTC TCATTTGTGA AAGTGGACTC ACACTTTCGA
    CAGGGAATTC CCTTCTTTGG GCAGGTGCGC CTAGTAGATG GGAAAGGCGT CCCTATACCA
    AATAAAGTCA TATTCATCAG AGGAAATGAA GCAAACTATT ACTCCAATGC TACCACGGAT
    GAGCATGGCC TTGTACAGTT CTCTATCAAC ACCACCAATG TTATGGGTAC CTCTCTTACT
    GTTAGGGTCA ATTACAAGGA TCGTAGTCCC TGTTACGGCT ACCAGTGGGT GTCAGAAGAA
    CACGAAGAGG CACATCACAC TGCTTATCTT GTGTTCTCCC CAAGCAAGAG CTTTGTCCAC
    CTTGAGCCCA TGTCTCATGA ACTACCCTGT GGCCATACTC AGACAGTCCA GGCACATTAT
    ATTCTGAATG GAGGCACCCT GCTGGGGCTG AAGAAGCTCT CCTTCTATTA TCTGATAATG
    GCAAAGGGAG GCATTGTCCG AACTGGGACT CATGGACTGC TTGTGAAGCA GGAAGACATG
    AAGGGCCATT TTTCCATCTC AATCCCTGTG AAGTCAGACA TTGCTCCTGT CGCTCGGTTG
    CTCATCTATG CTGTTTTACC TACCGGGGAC GTGATTGGGG ATTCTGCAAA ATATGATGTT
    GAAAATTGTC TGGCCAACAA GGTGGATTTG AGCTTCAGCC CATCACAAAG TCTCCCAGCC
    TCACACGCCC ACCTGCGAGT CACAGCGGCT CCTCAGTCCG TCTGCGCCCT CCGTGCTGTG
    GACCAAAGCG TGCTGCTCAT GAAGCCTGAT GCTGAGCTCT CGGCGTCCTC GGTTTACAAC
    CTGCTACCAG AAAAGGACCT CACTGGCTTC CCTGGGCCTT TGAATGACCA GGACAATGAA
    GACTGCATCA ATCGTCATAA TGTCTATATT AATGGAATCA CATATACTCC AGTATCAAGT
    ACAAATGAAA AGGATATGTA CAGCTTCCTA GAGGACATGG GCTTAAAGGC ATTCACCAAC
    TCAAAGATTC GTAAACCCAA AATGTGTCCA CAGCTTCAAC AGTATGAAAT GCATGGACCT
    GAAGGTCTAC GTGTAGGTTT TTATGAGTCA GATGTAATGG GAAGAGGCCA TGCACGCCTG
    GTGCATGTTG AAGAGCCTCA CACGGAGACC GTACGAAAGT ACTTCCCTGA GACATGGATC
    TGGGATTTGG TGGTGGTAAA CTCAGCAGGT GTGGCTGAGG TAGGAGTAAC AGTCCCTGAC
    ACCATCACCG AGTGGAAGGC AGGGGCCTTC TGCCTGTCTG AAGATGCTGG ACTTGGTATC
    TCTTCCACTG CCTCTCTCCG AGCCTTCCAG CCCTTCTTTG TGGAGCTCAC AATGCCTTAC
    TCTGTGATTC GTGGAGAGGC CTTCACACTC AAGGCCACGG TCCTAAACTA CCTTCCCAAA
    TGCATCCGGG TCAGTGTGCA GCTGGAAGCC TCTCCCGCCT TCCTAGCTGT CCCAGTGGAG
    AAGGAACAAG CGCCTCACTG CATCTGTGCA AACGGGCGGC AAACTGTGTC CTGGGCAGTA
    ACCCCAAAGT CATTAGGAAA TGTGAATTTC ACTGTGAGCG CAGAGGCACT AGAGTCTCAA
    GAGCTGTGTG GGACTGAGGT GCCTTCAGTT CCTGAACACG GAAGGAAAGA CACAGTCATC
    AAGCCTCTGT TGGTTGAACC TGAAGGACTA GAGAAGGAAA CAACATTCAA CTCCCTACTT
    TGTCCATCAG GTGGTGAGGT TTCTGAAGAA TTATCCCTGA AACTGCCACC AAATGTGGTA
    GAAGAATCTG CCCGAGCTTC TGTCTCAGTT TTGGGAGACA TATTAGGCTC TGCCATGCAA
    AACACACAAA ATCTTCTCCA GATGCCCTAT GGCTGTGGAG AGCAGAATAT GGTCCTCTTT
    GCTCCTAACA TCTATGTACT GGATTATCTA AATGAAACAC AGCAGCTTAC TCCAGAGATC
    AAGTCCAAGG CCATTGGCTA TCTCAACACT GGTTACCAGA GACAGTTGAA CTACAAACAC
    TATGATGGCT CCTACAGCAC CTTTGGGGAG CGATATGGCA GGAACCAGGG CAACACCTGG
    CTCACAGCCT TTGTTCTGAA GACTTTTGCC CAAGCTCGAG CCTACATCTT CATCGATGAA
    GCACACATTA CCCAAGCCCT CATATGGCTC TCCCAGAGGC AGAAGGACAA TGGCTGTTTC
    AGGAGCTCTG GGTCACTGCT CAACAATGCC ATAAAGGGAG GAGTAGAAGA TGAAGTGACC
    CTCTCCGCCT ATATCACCAT CGCCCTTCTG GAGATTCCTC TCACAGTCAC TCACCCTGTT
    GTCCGCAATG CCCTGTTTTG CCTGGAGTCA GCCTGGAAGA CAGCACAAGA AGGGGACCAT
    GGCAGCCATG TATATACCAA AGCACTGCTG GCCTATGCTT TTGCCCTGGC AGGTAACCAG
    GACAAGAGGA AGGAAGTACT CAAGTCACTT AATGAGGAAG CTGTGAAGAA AGACAACTCT
    GTCCATTGGG AGCGCCCTCA GAAACCCAAG GCACCAGTGG GGCATTTTTA CGAACCCCAG
    GCTCCCTCTG CTGAGGTGGA GATGACATCC TATGTGCTCC TCGCTTATCT CACGGCCCAG
    CCAGCCCCAA CCTCGGAGGA CCTGACCTCT GCAACCAACA TCGTGAAGTG GATCACGAAG
    CAGCAGAATG CCCAGGGCGG TTTCTCCTCC ACCCAGGACA CAGTGGTGGC TCTCCATGCT
    CTGTCCAAAT ATGGAGCAGC CACATTTACC AGGACTGGGA AGGCTGCACA GGTGACTATC
    CAGTCTTCAG GGACATTTTC CAGCAAATTC CAAGTGGACA ACAACAACCG CCTGTTACTG
    CAGCAGGTCT CATTGCCAGA GCTGCCTGGG GAATACAGCA TGAAAGTGAC AGGAGAAGGA
    TGTGTCTACC TCCAGACATC CTTGAAATAC AATATTCTCC CAGAAAAGGA AGAGTTCCCC
    TTTGCTTTAG GAGTGCAGAC TCTGCCTCAA ACTTGTGATG AACCCAAAGC CCACACCAGC
    TTCCAAATCT CCCTAAGTGT CAGTTACACA GGGAGCCGCT CTGCCTCCAA CATGGCGATC
    GTTGATGTGA AGATGGTCTC TGGCTTCATT CCCCTGAAGC CAACAGTGAA AATGCTTGAA
    AGATCTAACC ATGTGAGCCG GACAGAAGTC AGCAGCAACC ATGTCTTGAT TTACCTTGAT
    AAGGTGTCAA ATCAGACACT GAGCTTGTTC TTCACGGTTC TGCAAGATGT CCCAGTAAGA
    GATCTGAAAC CAGCCATAGT GAAAGTCTAT GATTACTACG AGACGGATGA GTTTGCAATT
    GCTGAGTACA ATGCTCCTTG CAGCAAAGGT AAGCCACTCA CACTCCTCCA AAAGGCAGTC
    AGAGCTCCTT CAGCTTGCCC CCCAAACCTT CTCCTTCATA AAACGCTGGG TAAATATTTG
    TCAAAAACAT CAAATTACTC ACACTGCACA TTATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006719119.1
    CDS92..4630
    Translation

    Target ORF information:

    RefSeq Version XM_006719056.2
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha-2-macroglobulin (A2M), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006719056.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGGAAGA ACAAACTCCT TCATCCAAGT CTGGTTCTTC TCCTCTTGGT CCTCCTGCCC 
    ACAGACGCCT CAGTCTCTGG AAAACCGCAG TATATGGTTC TGGTCCCCTC CCTGCTCCAC
    ACTGAGACCA CTGAGAAGGG CTGTGTCCTT CTGAGCTACC TGAATGAGAC AGTGACTGTA
    AGTGCTTCCT TGGAGTCTGT CAGGGGAAAC AGGAGCCTCT TCACTGACCT GGAGGCGGAG
    AATGACGTAC TCCACTGTGT CGCCTTCGCT GTCCCAAAGT CTTCATCCAA TGAGGAGGTA
    ATGTTCCTCA CTGTCCAAGT GAAAGGACCA ACCCAAGAAT TTAAGAAGCG GACCACAGTG
    ATGGTTAAGA ACGAGGACAG TCTGGTCTTT GTCCAGACAG ACAAATCAAT CTACAAACCA
    GGGCAGACAG TGAAATTTCG TGTTGTCTCC ATGGATGAAA ACTTTCACCC CCTGAATGAG
    TTGATTCCAC TAGTATACAT TCAGGATCCC AAAGGAAATC GCATCGCACA ATGGCAGAGT
    TTCCAGTTAG AGGGTGGCCT CAAGCAATTT TCTTTTCCCC TCTCATCAGA GCCCTTCCAG
    GGCTCCTACA AGGTGGTGGT ACAGAAGAAA TCAGGTGGAA GGACAGAGCA CCCTTTCACC
    GTGGAGGAAT TTGTTCTTCC CAAGTTTGAA GTACAAGTAA CAGTGCCAAA GATAATCACC
    ATCTTGGAAG AAGAGATGAA TGTATCAGTG TGTGGCCTAT ACACATATGG GAAGCCTGTC
    CCTGGACATG TGACTGTGAG CATTTGCAGA AAGTATAGTG ACGCTTCCGA CTGCCACGGT
    GAAGATTCAC AGGCTTTCTG TGAGAAATTC AGTGGACAGC TAAACAGCCA TGGCTGCTTC
    TATCAGCAAG TAAAAACCAA GGTCTTCCAG CTGAAGAGGA AGGAGTATGA AATGAAACTT
    CACACTGAGG CCCAGATCCA AGAAGAAGGA ACAGTGGTGG AATTGACTGG AAGGCAGTCC
    AGTGAAATCA CAAGAACCAT AACCAAACTC TCATTTGTGA AAGTGGACTC ACACTTTCGA
    CAGGGAATTC CCTTCTTTGG GCAGGTGCGC CTAGTAGATG GGAAAGGCGT CCCTATACCA
    AATAAAGTCA TATTCATCAG AGGAAATGAA GCAAACTATT ACTCCAATGC TACCACGGAT
    GAGCATGGCC TTGTACAGTT CTCTATCAAC ACCACCAATG TTATGGGTAC CTCTCTTACT
    GTTAGGGTCA ATTACAAGGA TCGTAGTCCC TGTTACGGCT ACCAGTGGGT GTCAGAAGAA
    CACGAAGAGG CACATCACAC TGCTTATCTT GTGTTCTCCC CAAGCAAGAG CTTTGTCCAC
    CTTGAGCCCA TGTCTCATGA ACTACCCTGT GGCCATACTC AGACAGTCCA GGCACATTAT
    ATTCTGAATG GAGGCACCCT GCTGGGGCTG AAGAAGCTCT CCTTCTATTA TCTGATAATG
    GCAAAGGGAG GCATTGTCCG AACTGGGACT CATGGACTGC TTGTGAAGCA GGAAGACATG
    AAGGGCCATT TTTCCATCTC AATCCCTGTG AAGTCAGACA TTGCTCCTGT CGCTCGGTTG
    CTCATCTATG CTGTTTTACC TACCGGGGAC GTGATTGGGG ATTCTGCAAA ATATGATGTT
    GAAAATTGTC TGGCCAACAA GGTGGATTTG AGCTTCAGCC CATCACAAAG TCTCCCAGCC
    TCACACGCCC ACCTGCGAGT CACAGCGGCT CCTCAGTCCG TCTGCGCCCT CCGTGCTGTG
    GACCAAAGCG TGCTGCTCAT GAAGCCTGAT GCTGAGCTCT CGGCGTCCTC GGTTTACAAC
    CTGCTACCAG AAAAGGACCT CACTGGCTTC CCTGGGCCTT TGAATGACCA GGACAATGAA
    GACTGCATCA ATCGTCATAA TGTCTATATT AATGGAATCA CATATACTCC AGTATCAAGT
    ACAAATGAAA AGGATATGTA CAGCTTCCTA GAGGACATGG GCTTAAAGGC ATTCACCAAC
    TCAAAGATTC GTAAACCCAA AATGTGTCCA CAGCTTCAAC AGTATGAAAT GCATGGACCT
    GAAGGTCTAC GTGTAGGTTT TTATGAGTCA GATGTAATGG GAAGAGGCCA TGCACGCCTG
    GTGCATGTTG AAGAGCCTCA CACGGAGACC GTACGAAAGT ACTTCCCTGA GACATGGATC
    TGGGATTTGG TGGTGGTAAA CTCAGCAGGT GTGGCTGAGG TAGGAGTAAC AGTCCCTGAC
    ACCATCACCG AGTGGAAGGC AGGGGCCTTC TGCCTGTCTG AAGATGCTGG ACTTGGTATC
    TCTTCCACTG CCTCTCTCCG AGCCTTCCAG CCCTTCTTTG TGGAGCTCAC AATGCCTTAC
    TCTGTGATTC GTGGAGAGGC CTTCACACTC AAGGCCACGG TCCTAAACTA CCTTCCCAAA
    TGCATCCGGG TCAGTGTGCA GCTGGAAGCC TCTCCCGCCT TCCTAGCTGT CCCAGTGGAG
    AAGGAACAAG CGCCTCACTG CATCTGTGCA AACGGGCGGC AAACTGTGTC CTGGGCAGTA
    ACCCCAAAGT CATTAGGAAA TGTGAATTTC ACTGTGAGCG CAGAGGCACT AGAGTCTCAA
    GAGCTGTGTG GGACTGAGGT GCCTTCAGTT CCTGAACACG GAAGGAAAGA CACAGTCATC
    AAGCCTCTGT TGGTTGAACC TGAAGGACTA GAGAAGGAAA CAACATTCAA CTCCCTACTT
    TGTCCATCAG GTGGTGAGGT TTCTGAAGAA TTATCCCTGA AACTGCCACC AAATGTGGTA
    GAAGAATCTG CCCGAGCTTC TGTCTCAGTT TTGGGAGACA TATTAGGCTC TGCCATGCAA
    AACACACAAA ATCTTCTCCA GATGCCCTAT GGCTGTGGAG AGCAGAATAT GGTCCTCTTT
    GCTCCTAACA TCTATGTACT GGATTATCTA AATGAAACAC AGCAGCTTAC TCCAGAGATC
    AAGTCCAAGG CCATTGGCTA TCTCAACACT GGTTACCAGA GACAGTTGAA CTACAAACAC
    TATGATGGCT CCTACAGCAC CTTTGGGGAG CGATATGGCA GGAACCAGGG CAACACCTGG
    CTCACAGCCT TTGTTCTGAA GACTTTTGCC CAAGCTCGAG CCTACATCTT CATCGATGAA
    GCACACATTA CCCAAGCCCT CATATGGCTC TCCCAGAGGC AGAAGGACAA TGGCTGTTTC
    AGGAGCTCTG GGTCACTGCT CAACAATGCC ATAAAGGGAG GAGTAGAAGA TGAAGTGACC
    CTCTCCGCCT ATATCACCAT CGCCCTTCTG GAGATTCCTC TCACAGTCAC TCACCCTGTT
    GTCCGCAATG CCCTGTTTTG CCTGGAGTCA GCCTGGAAGA CAGCACAAGA AGGGGACCAT
    GGCAGCCATG TATATACCAA AGCACTGCTG GCCTATGCTT TTGCCCTGGC AGGTAACCAG
    GACAAGAGGA AGGAAGTACT CAAGTCACTT AATGAGGAAG CTGTGAAGAA AGACAACTCT
    GTCCATTGGG AGCGCCCTCA GAAACCCAAG GCACCAGTGG GGCATTTTTA CGAACCCCAG
    GCTCCCTCTG CTGAGGTGGA GATGACATCC TATGTGCTCC TCGCTTATCT CACGGCCCAG
    CCAGCCCCAA CCTCGGAGGA CCTGACCTCT GCAACCAACA TCGTGAAGTG GATCACGAAG
    CAGCAGAATG CCCAGGGCGG TTTCTCCTCC ACCCAGGACA CAGTGGTGGC TCTCCATGCT
    CTGTCCAAAT ATGGAGCAGC CACATTTACC AGGACTGGGA AGGCTGCACA GGTGACTATC
    CAGTCTTCAG GGACATTTTC CAGCAAATTC CAAGTGGACA ACAACAACCG CCTGTTACTG
    CAGCAGGTCT CATTGCCAGA GCTGCCTGGG GAATACAGCA TGAAAGTGAC AGGAGAAGGA
    TGTGTCTACC TCCAGACATC CTTGAAATAC AATATTCTCC CAGAAAAGGA AGAGTTCCCC
    TTTGCTTTAG GAGTGCAGAC TCTGCCTCAA ACTTGTGATG AACCCAAAGC CCACACCAGC
    TTCCAAATCT CCCTAAGTGT CAGTTACACA GGGAGCCGCT CTGCCTCCAA CATGGCGATC
    GTTGATGTGA AGATGGTCTC TGGCTTCATT CCCCTGAAGC CAACAGTGAA AATGCTTGAA
    AGATCTAACC ATGTGAGCCG GACAGAAGTC AGCAGCAACC ATGTCTTGAT TTACCTTGAT
    AAGGTGTCAA ATCAGACACT GAGCTTGTTC TTCACGGTTC TGCAAGATGT CCCAGTAAGA
    GATCTGAAAC CAGCCATAGT GAAAGTCTAT GATTACTACG AGACGGATGA GTTTGCAATT
    GCTGAGTACA ATGCTCCTTG CAGCAAAGGT AAGCCACTCA CACTCCTCCA AAAGGCAGTC
    AGAGCTCCTT CAGCTTGCCC CCCAAACCTT CTCCTTCATA AAACGCTGGG TAAATATTTG
    TCAAAAACAT CAAATTACTC ACACTGCACA TTATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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