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A2M cDNA ORF clone, Homo sapiens(Human)

Gene Symbol A2M
Entrez Gene ID 2
Full Name alpha-2-macroglobulin
Synonyms A2MD,CPAMD5,FWP007,S863-7
General protein information
Preferred Names

alpha-2-macroglobulin

Names

alpha-2-macroglobulin
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
alpha-2-M

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

12

12p13.31

Summary The protein encoded by this gene is a protease inhibitor and cytokine transporter. It uses a bait-and-trap mechanism to inhibit a broad spectrum of proteases, including trypsin, thrombin and collagenase. It can also inhibit inflammatory cytokines, and it thus disrupts inflammatory cascades. Mutations in this gene are a cause of alpha-2-macroglobulin deficiency. This gene is implicated in Alzheimer's disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. A related pseudogene, which is also located on the p arm of chromosome 12, has been identified. [provided by RefSeq, Nov 2016].
Disorder MIM:

103950

Disorder Html: Emphysema due to alpha-2-macroglobulin deficiency (1); {Alzheimer

mRNA and Protein(s)

mRNA Protein Name
NM_001347423.1 NP_001334352.1 alpha-2-macroglobulin isoform a precursor
NM_001347425.1 NP_001334354.1 alpha-2-macroglobulin isoform c
NM_000014.5 NP_000005.2 alpha-2-macroglobulin isoform a precursor
NM_001347424.1 NP_001334353.1 alpha-2-macroglobulin isoform b
XM_006719056.2 XP_006719119.1 alpha-2-macroglobulin isoform X1



Rattus norvegicus (Norway rat) LOC100911545 XP_003749876.1
Danio rerio (zebrafish) LOC100003785 XP_005157599.1
Danio rerio (zebrafish) a2ml NP_001132951.1
Danio rerio (zebrafish) LOC100006925 XP_001921577.3
Danio rerio (zebrafish) LOC100006895 XP_001923676.1
Danio rerio (zebrafish) LOC100006782 XP_001345438.5
Danio rerio (zebrafish) a2ml1 XP_697477.6
Danio rerio (zebrafish) zgc:171446 NP_001108028.1
Danio rerio (zebrafish) sb:cb37 XP_001345541.5
Macaca mulatta (rhesus monkey) A2M XP_001114328.1
Homo sapiens (human) A2M NP_000005.2
Canis lupus familiaris (dog) A2M XP_534893.3
Bos taurus (cattle) A2M NP_001103265.1
Danio rerio (zebrafish) zgc:171426 XP_003198073.1
Danio rerio (zebrafish) zgc:165453 XP_001918704.3
Pan troglodytes (chimpanzee) A2M XP_001139819.1
Danio rerio (zebrafish) zgc:171445 NP_001107101.1
Mus musculus (house mouse) A2m NP_783327.2
Rattus norvegicus (Norway rat) A2m NP_036620.2
Gallus gallus (chicken) A2M XP_416476.3
Danio rerio (zebrafish) zgc:165518 NP_001093623.2
Danio rerio (zebrafish) si:dkey-52d15.2 XP_001345569.3
Danio rerio (zebrafish) si:dkey-46g23.5 NP_001188334.1
Danio rerio (zebrafish) si:dkey-46g23.2 XP_001332356.1
Xenopus tropicalis (tropical clawed frog) LOC100489060 XP_002938549.2
Xenopus tropicalis (tropical clawed frog) a2m XP_002938550.2


Related articles in PubMed

HK2 Proximal Tubule Epithelial Cells Synthesize and Secrete Plasma Proteins Predominantly Through the Apical Surface.
Zhao KW, Murray EJ, Murray SS
Journal of cellular biochemistry118(4)924-933(2017 Apr)

Inactivation of viruses by pasteurization at 60 °C for 10 h with and without 40% glucose as stabilizer during a new manufacturing process of α2-Macroglobulin from Cohn Fraction IV.
Huangfu C, Ma Y, Jia J, Lv M, Zhu F, Ma X, Zhao X, Zhang J
Biologicals : journal of the International Association of Biological Standardization46139-142(2017 Mar)

Alpha-2 macroglobulin in Alzheimer's disease: a marker of neuronal injury through the RCAN1 pathway.
Varma VR, Varma S, An Y, Hohman TJ, Seddighi S, Casanova R, Beri A, Dammer EB, Seyfried NT, Pletnikova O, Moghekar A, Wilson MR, Lah JJ, O'Brien RJ, Levey AI, Troncoso JC, Albert MS, Thambisetty M
Molecular psychiatry22(1)13-23(2017 Jan)

Mutations in CPAMD8 Cause a Unique Form of Autosomal-Recessive Anterior Segment Dysgenesis.
Cheong SS, Hentschel L, Davidson AE, Gerrelli D, Davie R, Rizzo R, Pontikos N, Plagnol V, Moore AT, Sowden JC, Michaelides M, Snead M, Tuft SJ, Hardcastle AJ
American journal of human genetics99(6)1338-1352(2016 Dec)

Primary structure of human alpha 2-macroglobulin. Complete disulfide bridge assignment and localization of two interchain bridges in the dimeric proteinase binding unit.
Jensen PE, Sottrup-Jensen L
The Journal of biological chemistry261(34)15863-9(1986 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Stabilizers during pasteurization improved alpha-2-macroglobulin (alpha2-M) activity by more than 300% points compared with the unstabilized control.
Title: Inactivation of viruses by pasteurization at 60 °C for 10 h with and without 40% glucose as stabilizer during a new manufacturing process of α2-Macroglobulin from Cohn Fraction IV.

The plasma level of alpha2M was found positively correlated, while HCII level was negatively correlated with higher vulnerability of carotid plaques. Both proteins were efficient in differentiating stable and vulnerable carotid plaques. These provide potential new targets for the research of carotid plaque vulnerability. Plasma alpha2M and HCII may be potential biomarkers for evaluation of the vulnerability of carotid ...
Title: Alpha-2-macroglobulin and heparin cofactor II and the vulnerability of carotid atherosclerotic plaques: An iTRAQ-based analysis.

This work demonstrates that alpha2M*/CS-GRP78 acts as an upstream regulator of the PDK1/PLK1 signaling axis to modulate c-MYC transcription and its target genes, suggesting a therapeutic strategy for targeting c-MYC-associated malignant progression.
Title: Activated α2-Macroglobulin Regulates Transcriptional Activation of c-MYC Target Genes through Cell Surface GRP78 Protein.

These data demonstrate that alpha2M* and LRP1 modulate the BKCachannel in human myometrium and that BKCaand its immunomodulatory interacting partners regulate Ca(2+)dynamics in hMSMCs during pregnancy.
Title: BKCa channel regulates calcium oscillations induced by alpha-2-macroglobulin in human myometrial smooth muscle cells.

Data show that low that low globulin (GLB) levels were significantly associated with favorable survival, and low ALB levels were associated with a significantly worse survival .
Title: Low pretreatment serum globulin may predict favorable prognosis for gastric cancer patients.

The following A2M gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the A2M cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu102221 NM_001347424.1
Latest version!
Homo sapiens alpha-2-macroglobulin (A2M), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $727.30
$1039.00
OHu102390 NM_001347425.1
Latest version!
Homo sapiens alpha-2-macroglobulin (A2M), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
OHu18488 NM_001347423.1
Latest version!
Homo sapiens alpha-2-macroglobulin (A2M), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu18488 NM_000014.5
Latest version!
Homo sapiens alpha-2-macroglobulin (A2M), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu46081 XM_006719056.2
Latest version!
Homo sapiens alpha-2-macroglobulin (A2M), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $811.30
$1159.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu102221
Clone ID Related Accession (Same CDS sequence) NM_001347424.1
Accession Version NM_001347424.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4125bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-24
Organism Homo sapiens(Human)
Product alpha-2-macroglobulin isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC334781.1, DC402196.1, DB288732.1, AC007436.1, BC040071.1 and AB209614.2. Transcript Variant: This variant (3) contains an alternate splice site in the 5' region, and it thus differs in its 5' UTR and initiates translation from a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.221045.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
ATGTTCCTCA CTGTCCAAGT GAAAGGACCA ACCCAAGAAT TTAAGAAGCG GACCACAGTG 
ATGGTTAAGA ACGAGGACAG TCTGGTCTTT GTCCAGACAG ACAAATCAAT CTACAAACCA
GGGCAGACAG TGAAATTTCG TGTTGTCTCC ATGGATGAAA ACTTTCACCC CCTGAATGAG
TTGATTCCAC TAGTATACAT TCAGGATCCC AAAGGAAATC GCATCGCACA ATGGCAGAGT
TTCCAGTTAG AGGGTGGCCT CAAGCAATTT TCTTTTCCCC TCTCATCAGA GCCCTTCCAG
GGCTCCTACA AGGTGGTGGT ACAGAAGAAA TCAGGTGGAA GGACAGAGCA CCCTTTCACC
GTGGAGGAAT TTGTTCTTCC CAAGTTTGAA GTACAAGTAA CAGTGCCAAA GATAATCACC
ATCTTGGAAG AAGAGATGAA TGTATCAGTG TGTGGCCTAT ACACATATGG GAAGCCTGTC
CCTGGACATG TGACTGTGAG CATTTGCAGA AAGTATAGTG ACGCTTCCGA CTGCCACGGT
GAAGATTCAC AGGCTTTCTG TGAGAAATTC AGTGGACAGC TAAACAGCCA TGGCTGCTTC
TATCAGCAAG TAAAAACCAA GGTCTTCCAG CTGAAGAGGA AGGAGTATGA AATGAAACTT
CACACTGAGG CCCAGATCCA AGAAGAAGGA ACAGTGGTGG AATTGACTGG AAGGCAGTCC
AGTGAAATCA CAAGAACCAT AACCAAACTC TCATTTGTGA AAGTGGACTC ACACTTTCGA
CAGGGAATTC CCTTCTTTGG GCAGGTGCGC CTAGTAGATG GGAAAGGCGT CCCTATACCA
AATAAAGTCA TATTCATCAG AGGAAATGAA GCAAACTATT ACTCCAATGC TACCACGGAT
GAGCATGGCC TTGTACAGTT CTCTATCAAC ACCACCAATG TTATGGGTAC CTCTCTTACT
GTTAGGGTCA ATTACAAGGA TCGTAGTCCC TGTTACGGCT ACCAGTGGGT GTCAGAAGAA
CACGAAGAGG CACATCACAC TGCTTATCTT GTGTTCTCCC CAAGCAAGAG CTTTGTCCAC
CTTGAGCCCA TGTCTCATGA ACTACCCTGT GGCCATACTC AGACAGTCCA GGCACATTAT
ATTCTGAATG GAGGCACCCT GCTGGGGCTG AAGAAGCTCT CCTTCTATTA TCTGATAATG
GCAAAGGGAG GCATTGTCCG AACTGGGACT CATGGACTGC TTGTGAAGCA GGAAGACATG
AAGGGCCATT TTTCCATCTC AATCCCTGTG AAGTCAGACA TTGCTCCTGT CGCTCGGTTG
CTCATCTATG CTGTTTTACC TACCGGGGAC GTGATTGGGG ATTCTGCAAA ATATGATGTT
GAAAATTGTC TGGCCAACAA GGTGGATTTG AGCTTCAGCC CATCACAAAG TCTCCCAGCC
TCACACGCCC ACCTGCGAGT CACAGCGGCT CCTCAGTCCG TCTGCGCCCT CCGTGCTGTG
GACCAAAGCG TGCTGCTCAT GAAGCCTGAT GCTGAGCTCT CGGCGTCCTC GGTTTACAAC
CTGCTACCAG AAAAGGACCT CACTGGCTTC CCTGGGCCTT TGAATGACCA GGACGATGAA
GACTGCATCA ATCGTCATAA TGTCTATATT AATGGAATCA CATATACTCC AGTATCAAGT
ACAAATGAAA AGGATATGTA CAGCTTCCTA GAGGACATGG GCTTAAAGGC ATTCACCAAC
TCAAAGATTC GTAAACCCAA AATGTGTCCA CAGCTTCAAC AGTATGAAAT GCATGGACCT
GAAGGTCTAC GTGTAGGTTT TTATGAGTCA GATGTAATGG GAAGAGGCCA TGCACGCCTG
GTGCATGTTG AAGAGCCTCA CACGGAGACC GTACGAAAGT ACTTCCCTGA GACATGGATC
TGGGATTTGG TGGTGGTAAA CTCAGCAGGT GTGGCTGAGG TAGGAGTAAC AGTCCCTGAC
ACCATCACCG AGTGGAAGGC AGGGGCCTTC TGCCTGTCTG AAGATGCTGG ACTTGGTATC
TCTTCCACTG CCTCTCTCCG AGCCTTCCAG CCCTTCTTTG TGGAGCTCAC AATGCCTTAC
TCTGTGATTC GTGGAGAGGC CTTCACACTC AAGGCCACGG TCCTAAACTA CCTTCCCAAA
TGCATCCGGG TCAGTGTGCA GCTGGAAGCC TCTCCCGCCT TCCTAGCTGT CCCAGTGGAG
AAGGAACAAG CGCCTCACTG CATCTGTGCA AACGGGCGGC AAACTGTGTC CTGGGCAGTA
ACCCCAAAGT CATTAGGAAA TGTGAATTTC ACTGTGAGCG CAGAGGCACT AGAGTCTCAA
GAGCTGTGTG GGACTGAGGT GCCTTCAGTT CCTGAACACG GAAGGAAAGA CACAGTCATC
AAGCCTCTGT TGGTTGAACC TGAAGGACTA GAGAAGGAAA CAACATTCAA CTCCCTACTT
TGTCCATCAG GTGGTGAGGT TTCTGAAGAA TTATCCCTGA AACTGCCACC AAATGTGGTA
GAAGAATCTG CCCGAGCTTC TGTCTCAGTT TTGGGAGACA TATTAGGCTC TGCCATGCAA
AACACACAAA ATCTTCTCCA GATGCCCTAT GGCTGTGGAG AGCAGAATAT GGTCCTCTTT
GCTCCTAACA TCTATGTACT GGATTATCTA AATGAAACAC AGCAGCTTAC TCCAGAGATC
AAGTCCAAGG CCATTGGCTA TCTCAACACT GGTTACCAGA GACAGTTGAA CTACAAACAC
TATGATGGCT CCTACAGCAC CTTTGGGGAG CGATATGGCA GGAACCAGGG CAACACCTGG
CTCACAGCCT TTGTTCTGAA GACTTTTGCC CAAGCTCGAG CCTACATCTT CATCGATGAA
GCACACATTA CCCAAGCCCT CATATGGCTC TCCCAGAGGC AGAAGGACAA TGGCTGTTTC
AGGAGCTCTG GGTCACTGCT CAACAATGCC ATAAAGGGAG GAGTAGAAGA TGAAGTGACC
CTCTCCGCCT ATATCACCAT CGCCCTTCTG GAGATTCCTC TCACAGTCAC TCACCCTGTT
GTCCGCAATG CCCTGTTTTG CCTGGAGTCA GCCTGGAAGA CAGCACAAGA AGGGGACCAT
GGCAGCCATG TATATACCAA AGCACTGCTG GCCTATGCTT TTGCCCTGGC AGGTAACCAG
GACAAGAGGA AGGAAGTACT CAAGTCACTT AATGAGGAAG CTGTGAAGAA AGACAACTCT
GTCCATTGGG AGCGCCCTCA GAAACCCAAG GCACCAGTGG GGCATTTTTA CGAACCCCAG
GCTCCCTCTG CTGAGGTGGA GATGACATCC TATGTGCTCC TCGCTTATCT CACGGCCCAG
CCAGCCCCAA CCTCGGAGGA CCTGACCTCT GCAACCAACA TCGTGAAGTG GATCACGAAG
CAGCAGAATG CCCAGGGCGG TTTCTCCTCC ACCCAGGACA CAGTGGTGGC TCTCCATGCT
CTGTCCAAAT ATGGAGCAGC CACATTTACC AGGACTGGGA AGGCTGCACA GGTGACTATC
CAGTCTTCAG GGACATTTTC CAGCAAATTC CAAGTGGACA ACAACAACCG CCTGTTACTG
CAGCAGGTCT CATTGCCAGA GCTGCCTGGG GAATACAGCA TGAAAGTGAC AGGAGAAGGA
TGTGTCTACC TCCAGACATC CTTGAAATAC AATATTCTCC CAGAAAAGGA AGAGTTCCCC
TTTGCTTTAG GAGTGCAGAC TCTGCCTCAA ACTTGTGATG AACCCAAAGC CCACACCAGC
TTCCAAATCT CCCTAAGTGT CAGTTACACA GGGAGCCGCT CTGCCTCCAA CATGGCGATC
GTTGATGTGA AGATGGTCTC TGGCTTCATT CCCCTGAAGC CAACAGTGAA AATGCTTGAA
AGATCTAACC ATGTGAGCCG GACAGAAGTC AGCAGCAACC ATGTCTTGAT TTACCTTGAT
AAGGTGTCAA ATCAGACACT GAGCTTGTTC TTCACGGTTC TGCAAGATGT CCCAGTAAGA
GATCTGAAAC CAGCCATAGT GAAAGTCTAT GATTACTACG AGACGGATGA GTTTGCAATT
GCTGAGTACA ATGCTCCTTG CAGCAAAGAT CTTGGAAATG CTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001334353.1
CDS834..4958
Misc Feature(1)747..749
Exon (1)1..466
Exon (2)467..650
Exon (3)651..963
Exon (4)964..1016
Exon (5)1017..1037
Exon (6)1038..1206
Exon (7)1207..1291
Exon (8)1292..1412
Exon (9)1413..1527
Exon (10)1528..1637
Exon (11)1638..1799
Exon (12)1800..2027
Exon (13)2028..2091
Exon (14)2092..2234
Exon (15)2235..2384
Exon (16)2385..2546
Exon (17)2547..2658
Exon (18)2659..2773
Exon (19)2774..3002
Exon (20)3003..3129
Exon (21)3130..3251
Exon (22)3252..3303
Exon (23)3304..3387
Exon (24)3388..3564
Exon (25)3565..3652
Exon (26)3653..3809
Exon (27)3810..3884
Exon (28)3885..4065
Exon (29)4066..4289
Exon (30)4290..4508
Exon (31)4509..4636
Exon (32)4637..4727
Exon (33)4728..4796
Exon (34)4797..4899
Exon (35)4900..4941
Exon (36)4942..5073
Translation

Target ORF information:

RefSeq Version NM_001347424.1
Organism Homo sapiens(Human)
Definition Homo sapiens alpha-2-macroglobulin (A2M), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001347424.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
ATGTTCCTCA CTGTCCAAGT GAAAGGACCA ACCCAAGAAT TTAAGAAGCG GACCACAGTG 
ATGGTTAAGA ACGAGGACAG TCTGGTCTTT GTCCAGACAG ACAAATCAAT CTACAAACCA
GGGCAGACAG TGAAATTTCG TGTTGTCTCC ATGGATGAAA ACTTTCACCC CCTGAATGAG
TTGATTCCAC TAGTATACAT TCAGGATCCC AAAGGAAATC GCATCGCACA ATGGCAGAGT
TTCCAGTTAG AGGGTGGCCT CAAGCAATTT TCTTTTCCCC TCTCATCAGA GCCCTTCCAG
GGCTCCTACA AGGTGGTGGT ACAGAAGAAA TCAGGTGGAA GGACAGAGCA CCCTTTCACC
GTGGAGGAAT TTGTTCTTCC CAAGTTTGAA GTACAAGTAA CAGTGCCAAA GATAATCACC
ATCTTGGAAG AAGAGATGAA TGTATCAGTG TGTGGCCTAT ACACATATGG GAAGCCTGTC
CCTGGACATG TGACTGTGAG CATTTGCAGA AAGTATAGTG ACGCTTCCGA CTGCCACGGT
GAAGATTCAC AGGCTTTCTG TGAGAAATTC AGTGGACAGC TAAACAGCCA TGGCTGCTTC
TATCAGCAAG TAAAAACCAA GGTCTTCCAG CTGAAGAGGA AGGAGTATGA AATGAAACTT
CACACTGAGG CCCAGATCCA AGAAGAAGGA ACAGTGGTGG AATTGACTGG AAGGCAGTCC
AGTGAAATCA CAAGAACCAT AACCAAACTC TCATTTGTGA AAGTGGACTC ACACTTTCGA
CAGGGAATTC CCTTCTTTGG GCAGGTGCGC CTAGTAGATG GGAAAGGCGT CCCTATACCA
AATAAAGTCA TATTCATCAG AGGAAATGAA GCAAACTATT ACTCCAATGC TACCACGGAT
GAGCATGGCC TTGTACAGTT CTCTATCAAC ACCACCAATG TTATGGGTAC CTCTCTTACT
GTTAGGGTCA ATTACAAGGA TCGTAGTCCC TGTTACGGCT ACCAGTGGGT GTCAGAAGAA
CACGAAGAGG CACATCACAC TGCTTATCTT GTGTTCTCCC CAAGCAAGAG CTTTGTCCAC
CTTGAGCCCA TGTCTCATGA ACTACCCTGT GGCCATACTC AGACAGTCCA GGCACATTAT
ATTCTGAATG GAGGCACCCT GCTGGGGCTG AAGAAGCTCT CCTTCTATTA TCTGATAATG
GCAAAGGGAG GCATTGTCCG AACTGGGACT CATGGACTGC TTGTGAAGCA GGAAGACATG
AAGGGCCATT TTTCCATCTC AATCCCTGTG AAGTCAGACA TTGCTCCTGT CGCTCGGTTG
CTCATCTATG CTGTTTTACC TACCGGGGAC GTGATTGGGG ATTCTGCAAA ATATGATGTT
GAAAATTGTC TGGCCAACAA GGTGGATTTG AGCTTCAGCC CATCACAAAG TCTCCCAGCC
TCACACGCCC ACCTGCGAGT CACAGCGGCT CCTCAGTCCG TCTGCGCCCT CCGTGCTGTG
GACCAAAGCG TGCTGCTCAT GAAGCCTGAT GCTGAGCTCT CGGCGTCCTC GGTTTACAAC
CTGCTACCAG AAAAGGACCT CACTGGCTTC CCTGGGCCTT TGAATGACCA GGACGATGAA
GACTGCATCA ATCGTCATAA TGTCTATATT AATGGAATCA CATATACTCC AGTATCAAGT
ACAAATGAAA AGGATATGTA CAGCTTCCTA GAGGACATGG GCTTAAAGGC ATTCACCAAC
TCAAAGATTC GTAAACCCAA AATGTGTCCA CAGCTTCAAC AGTATGAAAT GCATGGACCT
GAAGGTCTAC GTGTAGGTTT TTATGAGTCA GATGTAATGG GAAGAGGCCA TGCACGCCTG
GTGCATGTTG AAGAGCCTCA CACGGAGACC GTACGAAAGT ACTTCCCTGA GACATGGATC
TGGGATTTGG TGGTGGTAAA CTCAGCAGGT GTGGCTGAGG TAGGAGTAAC AGTCCCTGAC
ACCATCACCG AGTGGAAGGC AGGGGCCTTC TGCCTGTCTG AAGATGCTGG ACTTGGTATC
TCTTCCACTG CCTCTCTCCG AGCCTTCCAG CCCTTCTTTG TGGAGCTCAC AATGCCTTAC
TCTGTGATTC GTGGAGAGGC CTTCACACTC AAGGCCACGG TCCTAAACTA CCTTCCCAAA
TGCATCCGGG TCAGTGTGCA GCTGGAAGCC TCTCCCGCCT TCCTAGCTGT CCCAGTGGAG
AAGGAACAAG CGCCTCACTG CATCTGTGCA AACGGGCGGC AAACTGTGTC CTGGGCAGTA
ACCCCAAAGT CATTAGGAAA TGTGAATTTC ACTGTGAGCG CAGAGGCACT AGAGTCTCAA
GAGCTGTGTG GGACTGAGGT GCCTTCAGTT CCTGAACACG GAAGGAAAGA CACAGTCATC
AAGCCTCTGT TGGTTGAACC TGAAGGACTA GAGAAGGAAA CAACATTCAA CTCCCTACTT
TGTCCATCAG GTGGTGAGGT TTCTGAAGAA TTATCCCTGA AACTGCCACC AAATGTGGTA
GAAGAATCTG CCCGAGCTTC TGTCTCAGTT TTGGGAGACA TATTAGGCTC TGCCATGCAA
AACACACAAA ATCTTCTCCA GATGCCCTAT GGCTGTGGAG AGCAGAATAT GGTCCTCTTT
GCTCCTAACA TCTATGTACT GGATTATCTA AATGAAACAC AGCAGCTTAC TCCAGAGATC
AAGTCCAAGG CCATTGGCTA TCTCAACACT GGTTACCAGA GACAGTTGAA CTACAAACAC
TATGATGGCT CCTACAGCAC CTTTGGGGAG CGATATGGCA GGAACCAGGG CAACACCTGG
CTCACAGCCT TTGTTCTGAA GACTTTTGCC CAAGCTCGAG CCTACATCTT CATCGATGAA
GCACACATTA CCCAAGCCCT CATATGGCTC TCCCAGAGGC AGAAGGACAA TGGCTGTTTC
AGGAGCTCTG GGTCACTGCT CAACAATGCC ATAAAGGGAG GAGTAGAAGA TGAAGTGACC
CTCTCCGCCT ATATCACCAT CGCCCTTCTG GAGATTCCTC TCACAGTCAC TCACCCTGTT
GTCCGCAATG CCCTGTTTTG CCTGGAGTCA GCCTGGAAGA CAGCACAAGA AGGGGACCAT
GGCAGCCATG TATATACCAA AGCACTGCTG GCCTATGCTT TTGCCCTGGC AGGTAACCAG
GACAAGAGGA AGGAAGTACT CAAGTCACTT AATGAGGAAG CTGTGAAGAA AGACAACTCT
GTCCATTGGG AGCGCCCTCA GAAACCCAAG GCACCAGTGG GGCATTTTTA CGAACCCCAG
GCTCCCTCTG CTGAGGTGGA GATGACATCC TATGTGCTCC TCGCTTATCT CACGGCCCAG
CCAGCCCCAA CCTCGGAGGA CCTGACCTCT GCAACCAACA TCGTGAAGTG GATCACGAAG
CAGCAGAATG CCCAGGGCGG TTTCTCCTCC ACCCAGGACA CAGTGGTGGC TCTCCATGCT
CTGTCCAAAT ATGGAGCAGC CACATTTACC AGGACTGGGA AGGCTGCACA GGTGACTATC
CAGTCTTCAG GGACATTTTC CAGCAAATTC CAAGTGGACA ACAACAACCG CCTGTTACTG
CAGCAGGTCT CATTGCCAGA GCTGCCTGGG GAATACAGCA TGAAAGTGAC AGGAGAAGGA
TGTGTCTACC TCCAGACATC CTTGAAATAC AATATTCTCC CAGAAAAGGA AGAGTTCCCC
TTTGCTTTAG GAGTGCAGAC TCTGCCTCAA ACTTGTGATG AACCCAAAGC CCACACCAGC
TTCCAAATCT CCCTAAGTGT CAGTTACACA GGGAGCCGCT CTGCCTCCAA CATGGCGATC
GTTGATGTGA AGATGGTCTC TGGCTTCATT CCCCTGAAGC CAACAGTGAA AATGCTTGAA
AGATCTAACC ATGTGAGCCG GACAGAAGTC AGCAGCAACC ATGTCTTGAT TTACCTTGAT
AAGGTGTCAA ATCAGACACT GAGCTTGTTC TTCACGGTTC TGCAAGATGT CCCAGTAAGA
GATCTGAAAC CAGCCATAGT GAAAGTCTAT GATTACTACG AGACGGATGA GTTTGCAATT
GCTGAGTACA ATGCTCCTTG CAGCAAAGAT CTTGGAAATG CTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu102390
Clone ID Related Accession (Same CDS sequence) NM_001347425.1
Accession Version NM_001347425.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3975bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-24
Organism Homo sapiens(Human)
Product alpha-2-macroglobulin isoform c
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC334781.1, DC402196.1, AK307832.1 and BC040071.1. Transcript Variant: This variant (4) lacks an exon in the 5' region, and it thus differs in its 5' UTR and initiates translation from a downstream in-frame start codon, compared to variant 1. The encoded isoform (c) is shorter at the N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.96034.1, AK307832.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
ATGGATGAAA ACTTTCACCC CCTGAATGAG TTGATTCCAC TAGTATACAT TCAGGATCCC 
AAAGGAAATC GCATCGCACA ATGGCAGAGT TTCCAGTTAG AGGGTGGCCT CAAGCAATTT
TCTTTTCCCC TCTCATCAGA GCCCTTCCAG GGCTCCTACA AGGTGGTGGT ACAGAAGAAA
TCAGGTGGAA GGACAGAGCA CCCTTTCACC GTGGAGGAAT TTGTTCTTCC CAAGTTTGAA
GTACAAGTAA CAGTGCCAAA GATAATCACC ATCTTGGAAG AAGAGATGAA TGTATCAGTG
TGTGGCCTAT ACACATATGG GAAGCCTGTC CCTGGACATG TGACTGTGAG CATTTGCAGA
AAGTATAGTG ACGCTTCCGA CTGCCACGGT GAAGATTCAC AGGCTTTCTG TGAGAAATTC
AGTGGACAGC TAAACAGCCA TGGCTGCTTC TATCAGCAAG TAAAAACCAA GGTCTTCCAG
CTGAAGAGGA AGGAGTATGA AATGAAACTT CACACTGAGG CCCAGATCCA AGAAGAAGGA
ACAGTGGTGG AATTGACTGG AAGGCAGTCC AGTGAAATCA CAAGAACCAT AACCAAACTC
TCATTTGTGA AAGTGGACTC ACACTTTCGA CAGGGAATTC CCTTCTTTGG GCAGGTGCGC
CTAGTAGATG GGAAAGGCGT CCCTATACCA AATAAAGTCA TATTCATCAG AGGAAATGAA
GCAAACTATT ACTCCAATGC TACCACGGAT GAGCATGGCC TTGTACAGTT CTCTATCAAC
ACCACCAATG TTATGGGTAC CTCTCTTACT GTTAGGGTCA ATTACAAGGA TCGTAGTCCC
TGTTACGGCT ACCAGTGGGT GTCAGAAGAA CACGAAGAGG CACATCACAC TGCTTATCTT
GTGTTCTCCC CAAGCAAGAG CTTTGTCCAC CTTGAGCCCA TGTCTCATGA ACTACCCTGT
GGCCATACTC AGACAGTCCA GGCACATTAT ATTCTGAATG GAGGCACCCT GCTGGGGCTG
AAGAAGCTCT CCTTCTATTA TCTGATAATG GCAAAGGGAG GCATTGTCCG AACTGGGACT
CATGGACTGC TTGTGAAGCA GGAAGACATG AAGGGCCATT TTTCCATCTC AATCCCTGTG
AAGTCAGACA TTGCTCCTGT CGCTCGGTTG CTCATCTATG CTGTTTTACC TACCGGGGAC
GTGATTGGGG ATTCTGCAAA ATATGATGTT GAAAATTGTC TGGCCAACAA GGTGGATTTG
AGCTTCAGCC CATCACAAAG TCTCCCAGCC TCACACGCCC ACCTGCGAGT CACAGCGGCT
CCTCAGTCCG TCTGCGCCCT CCGTGCTGTG GACCAAAGCG TGCTGCTCAT GAAGCCTGAT
GCTGAGCTCT CGGCGTCCTC GGTTTACAAC CTGCTACCAG AAAAGGACCT CACTGGCTTC
CCTGGGCCTT TGAATGACCA GGACGATGAA GACTGCATCA ATCGTCATAA TGTCTATATT
AATGGAATCA CATATACTCC AGTATCAAGT ACAAATGAAA AGGATATGTA CAGCTTCCTA
GAGGACATGG GCTTAAAGGC ATTCACCAAC TCAAAGATTC GTAAACCCAA AATGTGTCCA
CAGCTTCAAC AGTATGAAAT GCATGGACCT GAAGGTCTAC GTGTAGGTTT TTATGAGTCA
GATGTAATGG GAAGAGGCCA TGCACGCCTG GTGCATGTTG AAGAGCCTCA CACGGAGACC
GTACGAAAGT ACTTCCCTGA GACATGGATC TGGGATTTGG TGGTGGTAAA CTCAGCAGGT
GTGGCTGAGG TAGGAGTAAC AGTCCCTGAC ACCATCACCG AGTGGAAGGC AGGGGCCTTC
TGCCTGTCTG AAGATGCTGG ACTTGGTATC TCTTCCACTG CCTCTCTCCG AGCCTTCCAG
CCCTTCTTTG TGGAGCTCAC AATGCCTTAC TCTGTGATTC GTGGAGAGGC CTTCACACTC
AAGGCCACGG TCCTAAACTA CCTTCCCAAA TGCATCCGGG TCAGTGTGCA GCTGGAAGCC
TCTCCCGCCT TCCTAGCTGT CCCAGTGGAG AAGGAACAAG CGCCTCACTG CATCTGTGCA
AACGGGCGGC AAACTGTGTC CTGGGCAGTA ACCCCAAAGT CATTAGGAAA TGTGAATTTC
ACTGTGAGCG CAGAGGCACT AGAGTCTCAA GAGCTGTGTG GGACTGAGGT GCCTTCAGTT
CCTGAACACG GAAGGAAAGA CACAGTCATC AAGCCTCTGT TGGTTGAACC TGAAGGACTA
GAGAAGGAAA CAACATTCAA CTCCCTACTT TGTCCATCAG GTGGTGAGGT TTCTGAAGAA
TTATCCCTGA AACTGCCACC AAATGTGGTA GAAGAATCTG CCCGAGCTTC TGTCTCAGTT
TTGGGAGACA TATTAGGCTC TGCCATGCAA AACACACAAA ATCTTCTCCA GATGCCCTAT
GGCTGTGGAG AGCAGAATAT GGTCCTCTTT GCTCCTAACA TCTATGTACT GGATTATCTA
AATGAAACAC AGCAGCTTAC TCCAGAGATC AAGTCCAAGG CCATTGGCTA TCTCAACACT
GGTTACCAGA GACAGTTGAA CTACAAACAC TATGATGGCT CCTACAGCAC CTTTGGGGAG
CGATATGGCA GGAACCAGGG CAACACCTGG CTCACAGCCT TTGTTCTGAA GACTTTTGCC
CAAGCTCGAG CCTACATCTT CATCGATGAA GCACACATTA CCCAAGCCCT CATATGGCTC
TCCCAGAGGC AGAAGGACAA TGGCTGTTTC AGGAGCTCTG GGTCACTGCT CAACAATGCC
ATAAAGGGAG GAGTAGAAGA TGAAGTGACC CTCTCCGCCT ATATCACCAT CGCCCTTCTG
GAGATTCCTC TCACAGTCAC TCACCCTGTT GTCCGCAATG CCCTGTTTTG CCTGGAGTCA
GCCTGGAAGA CAGCACAAGA AGGGGACCAT GGCAGCCATG TATATACCAA AGCACTGCTG
GCCTATGCTT TTGCCCTGGC AGGTAACCAG GACAAGAGGA AGGAAGTACT CAAGTCACTT
AATGAGGAAG CTGTGAAGAA AGACAACTCT GTCCATTGGG AGCGCCCTCA GAAACCCAAG
GCACCAGTGG GGCATTTTTA CGAACCCCAG GCTCCCTCTG CTGAGGTGGA GATGACATCC
TATGTGCTCC TCGCTTATCT CACGGCCCAG CCAGCCCCAA CCTCGGAGGA CCTGACCTCT
GCAACCAACA TCGTGAAGTG GATCACGAAG CAGCAGAATG CCCAGGGCGG TTTCTCCTCC
ACCCAGGACA CAGTGGTGGC TCTCCATGCT CTGTCCAAAT ATGGAGCAGC CACATTTACC
AGGACTGGGA AGGCTGCACA GGTGACTATC CAGTCTTCAG GGACATTTTC CAGCAAATTC
CAAGTGGACA ACAACAACCG CCTGTTACTG CAGCAGGTCT CATTGCCAGA GCTGCCTGGG
GAATACAGCA TGAAAGTGAC AGGAGAAGGA TGTGTCTACC TCCAGACATC CTTGAAATAC
AATATTCTCC CAGAAAAGGA AGAGTTCCCC TTTGCTTTAG GAGTGCAGAC TCTGCCTCAA
ACTTGTGATG AACCCAAAGC CCACACCAGC TTCCAAATCT CCCTAAGTGT CAGTTACACA
GGGAGCCGCT CTGCCTCCAA CATGGCGATC GTTGATGTGA AGATGGTCTC TGGCTTCATT
CCCCTGAAGC CAACAGTGAA AATGCTTGAA AGATCTAACC ATGTGAGCCG GACAGAAGTC
AGCAGCAACC ATGTCTTGAT TTACCTTGAT AAGGTGTCAA ATCAGACACT GAGCTTGTTC
TTCACGGTTC TGCAAGATGT CCCAGTAAGA GATCTGAAAC CAGCCATAGT GAAAGTCTAT
GATTACTACG AGACGGATGA GTTTGCAATT GCTGAGTACA ATGCTCCTTG CAGCAAAGAT
CTTGGAAATG CTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001334354.1
CDS671..4645
Misc Feature(1)623..625
Exon (1)1..466
Exon (2)467..650
Exon (3)651..703
Exon (4)704..724
Exon (5)725..893
Exon (6)894..978
Exon (7)979..1099
Exon (8)1100..1214
Exon (9)1215..1324
Exon (10)1325..1486
Exon (11)1487..1714
Exon (12)1715..1778
Exon (13)1779..1921
Exon (14)1922..2071
Exon (15)2072..2233
Exon (16)2234..2345
Exon (17)2346..2460
Exon (18)2461..2689
Exon (19)2690..2816
Exon (20)2817..2938
Exon (21)2939..2990
Exon (22)2991..3074
Exon (23)3075..3251
Exon (24)3252..3339
Exon (25)3340..3496
Exon (26)3497..3571
Exon (27)3572..3752
Exon (28)3753..3976
Exon (29)3977..4195
Exon (30)4196..4323
Exon (31)4324..4414
Exon (32)4415..4483
Exon (33)4484..4586
Exon (34)4587..4628
Exon (35)4629..4760
Translation

Target ORF information:

RefSeq Version NM_001347425.1
Organism Homo sapiens(Human)
Definition Homo sapiens alpha-2-macroglobulin (A2M), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001347425.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
ATGGATGAAA ACTTTCACCC CCTGAATGAG TTGATTCCAC TAGTATACAT TCAGGATCCC 
AAAGGAAATC GCATCGCACA ATGGCAGAGT TTCCAGTTAG AGGGTGGCCT CAAGCAATTT
TCTTTTCCCC TCTCATCAGA GCCCTTCCAG GGCTCCTACA AGGTGGTGGT ACAGAAGAAA
TCAGGTGGAA GGACAGAGCA CCCTTTCACC GTGGAGGAAT TTGTTCTTCC CAAGTTTGAA
GTACAAGTAA CAGTGCCAAA GATAATCACC ATCTTGGAAG AAGAGATGAA TGTATCAGTG
TGTGGCCTAT ACACATATGG GAAGCCTGTC CCTGGACATG TGACTGTGAG CATTTGCAGA
AAGTATAGTG ACGCTTCCGA CTGCCACGGT GAAGATTCAC AGGCTTTCTG TGAGAAATTC
AGTGGACAGC TAAACAGCCA TGGCTGCTTC TATCAGCAAG TAAAAACCAA GGTCTTCCAG
CTGAAGAGGA AGGAGTATGA AATGAAACTT CACACTGAGG CCCAGATCCA AGAAGAAGGA
ACAGTGGTGG AATTGACTGG AAGGCAGTCC AGTGAAATCA CAAGAACCAT AACCAAACTC
TCATTTGTGA AAGTGGACTC ACACTTTCGA CAGGGAATTC CCTTCTTTGG GCAGGTGCGC
CTAGTAGATG GGAAAGGCGT CCCTATACCA AATAAAGTCA TATTCATCAG AGGAAATGAA
GCAAACTATT ACTCCAATGC TACCACGGAT GAGCATGGCC TTGTACAGTT CTCTATCAAC
ACCACCAATG TTATGGGTAC CTCTCTTACT GTTAGGGTCA ATTACAAGGA TCGTAGTCCC
TGTTACGGCT ACCAGTGGGT GTCAGAAGAA CACGAAGAGG CACATCACAC TGCTTATCTT
GTGTTCTCCC CAAGCAAGAG CTTTGTCCAC CTTGAGCCCA TGTCTCATGA ACTACCCTGT
GGCCATACTC AGACAGTCCA GGCACATTAT ATTCTGAATG GAGGCACCCT GCTGGGGCTG
AAGAAGCTCT CCTTCTATTA TCTGATAATG GCAAAGGGAG GCATTGTCCG AACTGGGACT
CATGGACTGC TTGTGAAGCA GGAAGACATG AAGGGCCATT TTTCCATCTC AATCCCTGTG
AAGTCAGACA TTGCTCCTGT CGCTCGGTTG CTCATCTATG CTGTTTTACC TACCGGGGAC
GTGATTGGGG ATTCTGCAAA ATATGATGTT GAAAATTGTC TGGCCAACAA GGTGGATTTG
AGCTTCAGCC CATCACAAAG TCTCCCAGCC TCACACGCCC ACCTGCGAGT CACAGCGGCT
CCTCAGTCCG TCTGCGCCCT CCGTGCTGTG GACCAAAGCG TGCTGCTCAT GAAGCCTGAT
GCTGAGCTCT CGGCGTCCTC GGTTTACAAC CTGCTACCAG AAAAGGACCT CACTGGCTTC
CCTGGGCCTT TGAATGACCA GGACGATGAA GACTGCATCA ATCGTCATAA TGTCTATATT
AATGGAATCA CATATACTCC AGTATCAAGT ACAAATGAAA AGGATATGTA CAGCTTCCTA
GAGGACATGG GCTTAAAGGC ATTCACCAAC TCAAAGATTC GTAAACCCAA AATGTGTCCA
CAGCTTCAAC AGTATGAAAT GCATGGACCT GAAGGTCTAC GTGTAGGTTT TTATGAGTCA
GATGTAATGG GAAGAGGCCA TGCACGCCTG GTGCATGTTG AAGAGCCTCA CACGGAGACC
GTACGAAAGT ACTTCCCTGA GACATGGATC TGGGATTTGG TGGTGGTAAA CTCAGCAGGT
GTGGCTGAGG TAGGAGTAAC AGTCCCTGAC ACCATCACCG AGTGGAAGGC AGGGGCCTTC
TGCCTGTCTG AAGATGCTGG ACTTGGTATC TCTTCCACTG CCTCTCTCCG AGCCTTCCAG
CCCTTCTTTG TGGAGCTCAC AATGCCTTAC TCTGTGATTC GTGGAGAGGC CTTCACACTC
AAGGCCACGG TCCTAAACTA CCTTCCCAAA TGCATCCGGG TCAGTGTGCA GCTGGAAGCC
TCTCCCGCCT TCCTAGCTGT CCCAGTGGAG AAGGAACAAG CGCCTCACTG CATCTGTGCA
AACGGGCGGC AAACTGTGTC CTGGGCAGTA ACCCCAAAGT CATTAGGAAA TGTGAATTTC
ACTGTGAGCG CAGAGGCACT AGAGTCTCAA GAGCTGTGTG GGACTGAGGT GCCTTCAGTT
CCTGAACACG GAAGGAAAGA CACAGTCATC AAGCCTCTGT TGGTTGAACC TGAAGGACTA
GAGAAGGAAA CAACATTCAA CTCCCTACTT TGTCCATCAG GTGGTGAGGT TTCTGAAGAA
TTATCCCTGA AACTGCCACC AAATGTGGTA GAAGAATCTG CCCGAGCTTC TGTCTCAGTT
TTGGGAGACA TATTAGGCTC TGCCATGCAA AACACACAAA ATCTTCTCCA GATGCCCTAT
GGCTGTGGAG AGCAGAATAT GGTCCTCTTT GCTCCTAACA TCTATGTACT GGATTATCTA
AATGAAACAC AGCAGCTTAC TCCAGAGATC AAGTCCAAGG CCATTGGCTA TCTCAACACT
GGTTACCAGA GACAGTTGAA CTACAAACAC TATGATGGCT CCTACAGCAC CTTTGGGGAG
CGATATGGCA GGAACCAGGG CAACACCTGG CTCACAGCCT TTGTTCTGAA GACTTTTGCC
CAAGCTCGAG CCTACATCTT CATCGATGAA GCACACATTA CCCAAGCCCT CATATGGCTC
TCCCAGAGGC AGAAGGACAA TGGCTGTTTC AGGAGCTCTG GGTCACTGCT CAACAATGCC
ATAAAGGGAG GAGTAGAAGA TGAAGTGACC CTCTCCGCCT ATATCACCAT CGCCCTTCTG
GAGATTCCTC TCACAGTCAC TCACCCTGTT GTCCGCAATG CCCTGTTTTG CCTGGAGTCA
GCCTGGAAGA CAGCACAAGA AGGGGACCAT GGCAGCCATG TATATACCAA AGCACTGCTG
GCCTATGCTT TTGCCCTGGC AGGTAACCAG GACAAGAGGA AGGAAGTACT CAAGTCACTT
AATGAGGAAG CTGTGAAGAA AGACAACTCT GTCCATTGGG AGCGCCCTCA GAAACCCAAG
GCACCAGTGG GGCATTTTTA CGAACCCCAG GCTCCCTCTG CTGAGGTGGA GATGACATCC
TATGTGCTCC TCGCTTATCT CACGGCCCAG CCAGCCCCAA CCTCGGAGGA CCTGACCTCT
GCAACCAACA TCGTGAAGTG GATCACGAAG CAGCAGAATG CCCAGGGCGG TTTCTCCTCC
ACCCAGGACA CAGTGGTGGC TCTCCATGCT CTGTCCAAAT ATGGAGCAGC CACATTTACC
AGGACTGGGA AGGCTGCACA GGTGACTATC CAGTCTTCAG GGACATTTTC CAGCAAATTC
CAAGTGGACA ACAACAACCG CCTGTTACTG CAGCAGGTCT CATTGCCAGA GCTGCCTGGG
GAATACAGCA TGAAAGTGAC AGGAGAAGGA TGTGTCTACC TCCAGACATC CTTGAAATAC
AATATTCTCC CAGAAAAGGA AGAGTTCCCC TTTGCTTTAG GAGTGCAGAC TCTGCCTCAA
ACTTGTGATG AACCCAAAGC CCACACCAGC TTCCAAATCT CCCTAAGTGT CAGTTACACA
GGGAGCCGCT CTGCCTCCAA CATGGCGATC GTTGATGTGA AGATGGTCTC TGGCTTCATT
CCCCTGAAGC CAACAGTGAA AATGCTTGAA AGATCTAACC ATGTGAGCCG GACAGAAGTC
AGCAGCAACC ATGTCTTGAT TTACCTTGAT AAGGTGTCAA ATCAGACACT GAGCTTGTTC
TTCACGGTTC TGCAAGATGT CCCAGTAAGA GATCTGAAAC CAGCCATAGT GAAAGTCTAT
GATTACTACG AGACGGATGA GTTTGCAATT GCTGAGTACA ATGCTCCTTG CAGCAAAGAT
CTTGGAAATG CTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18488
Clone ID Related Accession (Same CDS sequence) NM_001347423.1 , NM_000014.5
Accession Version NM_001347423.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4425bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-24
Organism Homo sapiens(Human)
Product alpha-2-macroglobulin isoform a precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC334781.1, BC040071.1 and AB209614.2. Transcript Variant: This variant (2) splices out a region of the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.140912.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGGGGAAGA ACAAACTCCT TCATCCAAGT CTGGTTCTTC TCCTCTTGGT CCTCCTGCCC 
ACAGACGCCT CAGTCTCTGG AAAACCGCAG TATATGGTTC TGGTCCCCTC CCTGCTCCAC
ACTGAGACCA CTGAGAAGGG CTGTGTCCTT CTGAGCTACC TGAATGAGAC AGTGACTGTA
AGTGCTTCCT TGGAGTCTGT CAGGGGAAAC AGGAGCCTCT TCACTGACCT GGAGGCGGAG
AATGACGTAC TCCACTGTGT CGCCTTCGCT GTCCCAAAGT CTTCATCCAA TGAGGAGGTA
ATGTTCCTCA CTGTCCAAGT GAAAGGACCA ACCCAAGAAT TTAAGAAGCG GACCACAGTG
ATGGTTAAGA ACGAGGACAG TCTGGTCTTT GTCCAGACAG ACAAATCAAT CTACAAACCA
GGGCAGACAG TGAAATTTCG TGTTGTCTCC ATGGATGAAA ACTTTCACCC CCTGAATGAG
TTGATTCCAC TAGTATACAT TCAGGATCCC AAAGGAAATC GCATCGCACA ATGGCAGAGT
TTCCAGTTAG AGGGTGGCCT CAAGCAATTT TCTTTTCCCC TCTCATCAGA GCCCTTCCAG
GGCTCCTACA AGGTGGTGGT ACAGAAGAAA TCAGGTGGAA GGACAGAGCA CCCTTTCACC
GTGGAGGAAT TTGTTCTTCC CAAGTTTGAA GTACAAGTAA CAGTGCCAAA GATAATCACC
ATCTTGGAAG AAGAGATGAA TGTATCAGTG TGTGGCCTAT ACACATATGG GAAGCCTGTC
CCTGGACATG TGACTGTGAG CATTTGCAGA AAGTATAGTG ACGCTTCCGA CTGCCACGGT
GAAGATTCAC AGGCTTTCTG TGAGAAATTC AGTGGACAGC TAAACAGCCA TGGCTGCTTC
TATCAGCAAG TAAAAACCAA GGTCTTCCAG CTGAAGAGGA AGGAGTATGA AATGAAACTT
CACACTGAGG CCCAGATCCA AGAAGAAGGA ACAGTGGTGG AATTGACTGG AAGGCAGTCC
AGTGAAATCA CAAGAACCAT AACCAAACTC TCATTTGTGA AAGTGGACTC ACACTTTCGA
CAGGGAATTC CCTTCTTTGG GCAGGTGCGC CTAGTAGATG GGAAAGGCGT CCCTATACCA
AATAAAGTCA TATTCATCAG AGGAAATGAA GCAAACTATT ACTCCAATGC TACCACGGAT
GAGCATGGCC TTGTACAGTT CTCTATCAAC ACCACCAATG TTATGGGTAC CTCTCTTACT
GTTAGGGTCA ATTACAAGGA TCGTAGTCCC TGTTACGGCT ACCAGTGGGT GTCAGAAGAA
CACGAAGAGG CACATCACAC TGCTTATCTT GTGTTCTCCC CAAGCAAGAG CTTTGTCCAC
CTTGAGCCCA TGTCTCATGA ACTACCCTGT GGCCATACTC AGACAGTCCA GGCACATTAT
ATTCTGAATG GAGGCACCCT GCTGGGGCTG AAGAAGCTCT CCTTCTATTA TCTGATAATG
GCAAAGGGAG GCATTGTCCG AACTGGGACT CATGGACTGC TTGTGAAGCA GGAAGACATG
AAGGGCCATT TTTCCATCTC AATCCCTGTG AAGTCAGACA TTGCTCCTGT CGCTCGGTTG
CTCATCTATG CTGTTTTACC TACCGGGGAC GTGATTGGGG ATTCTGCAAA ATATGATGTT
GAAAATTGTC TGGCCAACAA GGTGGATTTG AGCTTCAGCC CATCACAAAG TCTCCCAGCC
TCACACGCCC ACCTGCGAGT CACAGCGGCT CCTCAGTCCG TCTGCGCCCT CCGTGCTGTG
GACCAAAGCG TGCTGCTCAT GAAGCCTGAT GCTGAGCTCT CGGCGTCCTC GGTTTACAAC
CTGCTACCAG AAAAGGACCT CACTGGCTTC CCTGGGCCTT TGAATGACCA GGACGATGAA
GACTGCATCA ATCGTCATAA TGTCTATATT AATGGAATCA CATATACTCC AGTATCAAGT
ACAAATGAAA AGGATATGTA CAGCTTCCTA GAGGACATGG GCTTAAAGGC ATTCACCAAC
TCAAAGATTC GTAAACCCAA AATGTGTCCA CAGCTTCAAC AGTATGAAAT GCATGGACCT
GAAGGTCTAC GTGTAGGTTT TTATGAGTCA GATGTAATGG GAAGAGGCCA TGCACGCCTG
GTGCATGTTG AAGAGCCTCA CACGGAGACC GTACGAAAGT ACTTCCCTGA GACATGGATC
TGGGATTTGG TGGTGGTAAA CTCAGCAGGT GTGGCTGAGG TAGGAGTAAC AGTCCCTGAC
ACCATCACCG AGTGGAAGGC AGGGGCCTTC TGCCTGTCTG AAGATGCTGG ACTTGGTATC
TCTTCCACTG CCTCTCTCCG AGCCTTCCAG CCCTTCTTTG TGGAGCTCAC AATGCCTTAC
TCTGTGATTC GTGGAGAGGC CTTCACACTC AAGGCCACGG TCCTAAACTA CCTTCCCAAA
TGCATCCGGG TCAGTGTGCA GCTGGAAGCC TCTCCCGCCT TCCTAGCTGT CCCAGTGGAG
AAGGAACAAG CGCCTCACTG CATCTGTGCA AACGGGCGGC AAACTGTGTC CTGGGCAGTA
ACCCCAAAGT CATTAGGAAA TGTGAATTTC ACTGTGAGCG CAGAGGCACT AGAGTCTCAA
GAGCTGTGTG GGACTGAGGT GCCTTCAGTT CCTGAACACG GAAGGAAAGA CACAGTCATC
AAGCCTCTGT TGGTTGAACC TGAAGGACTA GAGAAGGAAA CAACATTCAA CTCCCTACTT
TGTCCATCAG GTGGTGAGGT TTCTGAAGAA TTATCCCTGA AACTGCCACC AAATGTGGTA
GAAGAATCTG CCCGAGCTTC TGTCTCAGTT TTGGGAGACA TATTAGGCTC TGCCATGCAA
AACACACAAA ATCTTCTCCA GATGCCCTAT GGCTGTGGAG AGCAGAATAT GGTCCTCTTT
GCTCCTAACA TCTATGTACT GGATTATCTA AATGAAACAC AGCAGCTTAC TCCAGAGATC
AAGTCCAAGG CCATTGGCTA TCTCAACACT GGTTACCAGA GACAGTTGAA CTACAAACAC
TATGATGGCT CCTACAGCAC CTTTGGGGAG CGATATGGCA GGAACCAGGG CAACACCTGG
CTCACAGCCT TTGTTCTGAA GACTTTTGCC CAAGCTCGAG CCTACATCTT CATCGATGAA
GCACACATTA CCCAAGCCCT CATATGGCTC TCCCAGAGGC AGAAGGACAA TGGCTGTTTC
AGGAGCTCTG GGTCACTGCT CAACAATGCC ATAAAGGGAG GAGTAGAAGA TGAAGTGACC
CTCTCCGCCT ATATCACCAT CGCCCTTCTG GAGATTCCTC TCACAGTCAC TCACCCTGTT
GTCCGCAATG CCCTGTTTTG CCTGGAGTCA GCCTGGAAGA CAGCACAAGA AGGGGACCAT
GGCAGCCATG TATATACCAA AGCACTGCTG GCCTATGCTT TTGCCCTGGC AGGTAACCAG
GACAAGAGGA AGGAAGTACT CAAGTCACTT AATGAGGAAG CTGTGAAGAA AGACAACTCT
GTCCATTGGG AGCGCCCTCA GAAACCCAAG GCACCAGTGG GGCATTTTTA CGAACCCCAG
GCTCCCTCTG CTGAGGTGGA GATGACATCC TATGTGCTCC TCGCTTATCT CACGGCCCAG
CCAGCCCCAA CCTCGGAGGA CCTGACCTCT GCAACCAACA TCGTGAAGTG GATCACGAAG
CAGCAGAATG CCCAGGGCGG TTTCTCCTCC ACCCAGGACA CAGTGGTGGC TCTCCATGCT
CTGTCCAAAT ATGGAGCAGC CACATTTACC AGGACTGGGA AGGCTGCACA GGTGACTATC
CAGTCTTCAG GGACATTTTC CAGCAAATTC CAAGTGGACA ACAACAACCG CCTGTTACTG
CAGCAGGTCT CATTGCCAGA GCTGCCTGGG GAATACAGCA TGAAAGTGAC AGGAGAAGGA
TGTGTCTACC TCCAGACATC CTTGAAATAC AATATTCTCC CAGAAAAGGA AGAGTTCCCC
TTTGCTTTAG GAGTGCAGAC TCTGCCTCAA ACTTGTGATG AACCCAAAGC CCACACCAGC
TTCCAAATCT CCCTAAGTGT CAGTTACACA GGGAGCCGCT CTGCCTCCAA CATGGCGATC
GTTGATGTGA AGATGGTCTC TGGCTTCATT CCCCTGAAGC CAACAGTGAA AATGCTTGAA
AGATCTAACC ATGTGAGCCG GACAGAAGTC AGCAGCAACC ATGTCTTGAT TTACCTTGAT
AAGGTGTCAA ATCAGACACT GAGCTTGTTC TTCACGGTTC TGCAAGATGT CCCAGTAAGA
GATCTGAAAC CAGCCATAGT GAAAGTCTAT GATTACTACG AGACGGATGA GTTTGCAATT
GCTGAGTACA ATGCTCCTTG CAGCAAAGAT CTTGGAAATG CTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001334352.1
CDS120..4544
Misc Feature(1)84..86
Misc Feature(2)2187..2303
Misc Feature(3)2229..2246
Misc Feature(4)2274..2288
Misc Feature(5)2307..2324
Exon (1)1..102
Exon (2)103..205
Exon (3)206..389
Exon (4)390..549
Exon (5)550..602
Exon (6)603..623
Exon (7)624..792
Exon (8)793..877
Exon (9)878..998
Exon (10)999..1113
Exon (11)1114..1223
Exon (12)1224..1385
Exon (13)1386..1613
Exon (14)1614..1677
Exon (15)1678..1820
Exon (16)1821..1970
Exon (17)1971..2132
Exon (18)2133..2244
Exon (19)2245..2359
Exon (20)2360..2588
Exon (21)2589..2715
Exon (22)2716..2837
Exon (23)2838..2889
Exon (24)2890..2973
Exon (25)2974..3150
Exon (26)3151..3238
Exon (27)3239..3395
Exon (28)3396..3470
Exon (29)3471..3651
Exon (30)3652..3875
Exon (31)3876..4094
Exon (32)4095..4222
Exon (33)4223..4313
Exon (34)4314..4382
Exon (35)4383..4485
Exon (36)4486..4527
Exon (37)4528..4659
Translation

Target ORF information:

RefSeq Version NM_001347423.1
Organism Homo sapiens(Human)
Definition Homo sapiens alpha-2-macroglobulin (A2M), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001347423.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGGGGAAGA ACAAACTCCT TCATCCAAGT CTGGTTCTTC TCCTCTTGGT CCTCCTGCCC 
ACAGACGCCT CAGTCTCTGG AAAACCGCAG TATATGGTTC TGGTCCCCTC CCTGCTCCAC
ACTGAGACCA CTGAGAAGGG CTGTGTCCTT CTGAGCTACC TGAATGAGAC AGTGACTGTA
AGTGCTTCCT TGGAGTCTGT CAGGGGAAAC AGGAGCCTCT TCACTGACCT GGAGGCGGAG
AATGACGTAC TCCACTGTGT CGCCTTCGCT GTCCCAAAGT CTTCATCCAA TGAGGAGGTA
ATGTTCCTCA CTGTCCAAGT GAAAGGACCA ACCCAAGAAT TTAAGAAGCG GACCACAGTG
ATGGTTAAGA ACGAGGACAG TCTGGTCTTT GTCCAGACAG ACAAATCAAT CTACAAACCA
GGGCAGACAG TGAAATTTCG TGTTGTCTCC ATGGATGAAA ACTTTCACCC CCTGAATGAG
TTGATTCCAC TAGTATACAT TCAGGATCCC AAAGGAAATC GCATCGCACA ATGGCAGAGT
TTCCAGTTAG AGGGTGGCCT CAAGCAATTT TCTTTTCCCC TCTCATCAGA GCCCTTCCAG
GGCTCCTACA AGGTGGTGGT ACAGAAGAAA TCAGGTGGAA GGACAGAGCA CCCTTTCACC
GTGGAGGAAT TTGTTCTTCC CAAGTTTGAA GTACAAGTAA CAGTGCCAAA GATAATCACC
ATCTTGGAAG AAGAGATGAA TGTATCAGTG TGTGGCCTAT ACACATATGG GAAGCCTGTC
CCTGGACATG TGACTGTGAG CATTTGCAGA AAGTATAGTG ACGCTTCCGA CTGCCACGGT
GAAGATTCAC AGGCTTTCTG TGAGAAATTC AGTGGACAGC TAAACAGCCA TGGCTGCTTC
TATCAGCAAG TAAAAACCAA GGTCTTCCAG CTGAAGAGGA AGGAGTATGA AATGAAACTT
CACACTGAGG CCCAGATCCA AGAAGAAGGA ACAGTGGTGG AATTGACTGG AAGGCAGTCC
AGTGAAATCA CAAGAACCAT AACCAAACTC TCATTTGTGA AAGTGGACTC ACACTTTCGA
CAGGGAATTC CCTTCTTTGG GCAGGTGCGC CTAGTAGATG GGAAAGGCGT CCCTATACCA
AATAAAGTCA TATTCATCAG AGGAAATGAA GCAAACTATT ACTCCAATGC TACCACGGAT
GAGCATGGCC TTGTACAGTT CTCTATCAAC ACCACCAATG TTATGGGTAC CTCTCTTACT
GTTAGGGTCA ATTACAAGGA TCGTAGTCCC TGTTACGGCT ACCAGTGGGT GTCAGAAGAA
CACGAAGAGG CACATCACAC TGCTTATCTT GTGTTCTCCC CAAGCAAGAG CTTTGTCCAC
CTTGAGCCCA TGTCTCATGA ACTACCCTGT GGCCATACTC AGACAGTCCA GGCACATTAT
ATTCTGAATG GAGGCACCCT GCTGGGGCTG AAGAAGCTCT CCTTCTATTA TCTGATAATG
GCAAAGGGAG GCATTGTCCG AACTGGGACT CATGGACTGC TTGTGAAGCA GGAAGACATG
AAGGGCCATT TTTCCATCTC AATCCCTGTG AAGTCAGACA TTGCTCCTGT CGCTCGGTTG
CTCATCTATG CTGTTTTACC TACCGGGGAC GTGATTGGGG ATTCTGCAAA ATATGATGTT
GAAAATTGTC TGGCCAACAA GGTGGATTTG AGCTTCAGCC CATCACAAAG TCTCCCAGCC
TCACACGCCC ACCTGCGAGT CACAGCGGCT CCTCAGTCCG TCTGCGCCCT CCGTGCTGTG
GACCAAAGCG TGCTGCTCAT GAAGCCTGAT GCTGAGCTCT CGGCGTCCTC GGTTTACAAC
CTGCTACCAG AAAAGGACCT CACTGGCTTC CCTGGGCCTT TGAATGACCA GGACGATGAA
GACTGCATCA ATCGTCATAA TGTCTATATT AATGGAATCA CATATACTCC AGTATCAAGT
ACAAATGAAA AGGATATGTA CAGCTTCCTA GAGGACATGG GCTTAAAGGC ATTCACCAAC
TCAAAGATTC GTAAACCCAA AATGTGTCCA CAGCTTCAAC AGTATGAAAT GCATGGACCT
GAAGGTCTAC GTGTAGGTTT TTATGAGTCA GATGTAATGG GAAGAGGCCA TGCACGCCTG
GTGCATGTTG AAGAGCCTCA CACGGAGACC GTACGAAAGT ACTTCCCTGA GACATGGATC
TGGGATTTGG TGGTGGTAAA CTCAGCAGGT GTGGCTGAGG TAGGAGTAAC AGTCCCTGAC
ACCATCACCG AGTGGAAGGC AGGGGCCTTC TGCCTGTCTG AAGATGCTGG ACTTGGTATC
TCTTCCACTG CCTCTCTCCG AGCCTTCCAG CCCTTCTTTG TGGAGCTCAC AATGCCTTAC
TCTGTGATTC GTGGAGAGGC CTTCACACTC AAGGCCACGG TCCTAAACTA CCTTCCCAAA
TGCATCCGGG TCAGTGTGCA GCTGGAAGCC TCTCCCGCCT TCCTAGCTGT CCCAGTGGAG
AAGGAACAAG CGCCTCACTG CATCTGTGCA AACGGGCGGC AAACTGTGTC CTGGGCAGTA
ACCCCAAAGT CATTAGGAAA TGTGAATTTC ACTGTGAGCG CAGAGGCACT AGAGTCTCAA
GAGCTGTGTG GGACTGAGGT GCCTTCAGTT CCTGAACACG GAAGGAAAGA CACAGTCATC
AAGCCTCTGT TGGTTGAACC TGAAGGACTA GAGAAGGAAA CAACATTCAA CTCCCTACTT
TGTCCATCAG GTGGTGAGGT TTCTGAAGAA TTATCCCTGA AACTGCCACC AAATGTGGTA
GAAGAATCTG CCCGAGCTTC TGTCTCAGTT TTGGGAGACA TATTAGGCTC TGCCATGCAA
AACACACAAA ATCTTCTCCA GATGCCCTAT GGCTGTGGAG AGCAGAATAT GGTCCTCTTT
GCTCCTAACA TCTATGTACT GGATTATCTA AATGAAACAC AGCAGCTTAC TCCAGAGATC
AAGTCCAAGG CCATTGGCTA TCTCAACACT GGTTACCAGA GACAGTTGAA CTACAAACAC
TATGATGGCT CCTACAGCAC CTTTGGGGAG CGATATGGCA GGAACCAGGG CAACACCTGG
CTCACAGCCT TTGTTCTGAA GACTTTTGCC CAAGCTCGAG CCTACATCTT CATCGATGAA
GCACACATTA CCCAAGCCCT CATATGGCTC TCCCAGAGGC AGAAGGACAA TGGCTGTTTC
AGGAGCTCTG GGTCACTGCT CAACAATGCC ATAAAGGGAG GAGTAGAAGA TGAAGTGACC
CTCTCCGCCT ATATCACCAT CGCCCTTCTG GAGATTCCTC TCACAGTCAC TCACCCTGTT
GTCCGCAATG CCCTGTTTTG CCTGGAGTCA GCCTGGAAGA CAGCACAAGA AGGGGACCAT
GGCAGCCATG TATATACCAA AGCACTGCTG GCCTATGCTT TTGCCCTGGC AGGTAACCAG
GACAAGAGGA AGGAAGTACT CAAGTCACTT AATGAGGAAG CTGTGAAGAA AGACAACTCT
GTCCATTGGG AGCGCCCTCA GAAACCCAAG GCACCAGTGG GGCATTTTTA CGAACCCCAG
GCTCCCTCTG CTGAGGTGGA GATGACATCC TATGTGCTCC TCGCTTATCT CACGGCCCAG
CCAGCCCCAA CCTCGGAGGA CCTGACCTCT GCAACCAACA TCGTGAAGTG GATCACGAAG
CAGCAGAATG CCCAGGGCGG TTTCTCCTCC ACCCAGGACA CAGTGGTGGC TCTCCATGCT
CTGTCCAAAT ATGGAGCAGC CACATTTACC AGGACTGGGA AGGCTGCACA GGTGACTATC
CAGTCTTCAG GGACATTTTC CAGCAAATTC CAAGTGGACA ACAACAACCG CCTGTTACTG
CAGCAGGTCT CATTGCCAGA GCTGCCTGGG GAATACAGCA TGAAAGTGAC AGGAGAAGGA
TGTGTCTACC TCCAGACATC CTTGAAATAC AATATTCTCC CAGAAAAGGA AGAGTTCCCC
TTTGCTTTAG GAGTGCAGAC TCTGCCTCAA ACTTGTGATG AACCCAAAGC CCACACCAGC
TTCCAAATCT CCCTAAGTGT CAGTTACACA GGGAGCCGCT CTGCCTCCAA CATGGCGATC
GTTGATGTGA AGATGGTCTC TGGCTTCATT CCCCTGAAGC CAACAGTGAA AATGCTTGAA
AGATCTAACC ATGTGAGCCG GACAGAAGTC AGCAGCAACC ATGTCTTGAT TTACCTTGAT
AAGGTGTCAA ATCAGACACT GAGCTTGTTC TTCACGGTTC TGCAAGATGT CCCAGTAAGA
GATCTGAAAC CAGCCATAGT GAAAGTCTAT GATTACTACG AGACGGATGA GTTTGCAATT
GCTGAGTACA ATGCTCCTTG CAGCAAAGAT CTTGGAAATG CTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18488
Clone ID Related Accession (Same CDS sequence) NM_001347423.1 , NM_000014.5
Accession Version NM_000014.5 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4425bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-24
Organism Homo sapiens(Human)
Product alpha-2-macroglobulin isoform a precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC334781.1, DC402196.1, BC040071.1 and AB209614.2. On Nov 26, 2016 this sequence version replaced NM_000014.4. Transcript Variant: This variant (1) encodes the longest isoform (a). Both variants 1 and 2 encode isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC040071.1, SRR1660809.61793.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGGGGAAGA ACAAACTCCT TCATCCAAGT CTGGTTCTTC TCCTCTTGGT CCTCCTGCCC 
ACAGACGCCT CAGTCTCTGG AAAACCGCAG TATATGGTTC TGGTCCCCTC CCTGCTCCAC
ACTGAGACCA CTGAGAAGGG CTGTGTCCTT CTGAGCTACC TGAATGAGAC AGTGACTGTA
AGTGCTTCCT TGGAGTCTGT CAGGGGAAAC AGGAGCCTCT TCACTGACCT GGAGGCGGAG
AATGACGTAC TCCACTGTGT CGCCTTCGCT GTCCCAAAGT CTTCATCCAA TGAGGAGGTA
ATGTTCCTCA CTGTCCAAGT GAAAGGACCA ACCCAAGAAT TTAAGAAGCG GACCACAGTG
ATGGTTAAGA ACGAGGACAG TCTGGTCTTT GTCCAGACAG ACAAATCAAT CTACAAACCA
GGGCAGACAG TGAAATTTCG TGTTGTCTCC ATGGATGAAA ACTTTCACCC CCTGAATGAG
TTGATTCCAC TAGTATACAT TCAGGATCCC AAAGGAAATC GCATCGCACA ATGGCAGAGT
TTCCAGTTAG AGGGTGGCCT CAAGCAATTT TCTTTTCCCC TCTCATCAGA GCCCTTCCAG
GGCTCCTACA AGGTGGTGGT ACAGAAGAAA TCAGGTGGAA GGACAGAGCA CCCTTTCACC
GTGGAGGAAT TTGTTCTTCC CAAGTTTGAA GTACAAGTAA CAGTGCCAAA GATAATCACC
ATCTTGGAAG AAGAGATGAA TGTATCAGTG TGTGGCCTAT ACACATATGG GAAGCCTGTC
CCTGGACATG TGACTGTGAG CATTTGCAGA AAGTATAGTG ACGCTTCCGA CTGCCACGGT
GAAGATTCAC AGGCTTTCTG TGAGAAATTC AGTGGACAGC TAAACAGCCA TGGCTGCTTC
TATCAGCAAG TAAAAACCAA GGTCTTCCAG CTGAAGAGGA AGGAGTATGA AATGAAACTT
CACACTGAGG CCCAGATCCA AGAAGAAGGA ACAGTGGTGG AATTGACTGG AAGGCAGTCC
AGTGAAATCA CAAGAACCAT AACCAAACTC TCATTTGTGA AAGTGGACTC ACACTTTCGA
CAGGGAATTC CCTTCTTTGG GCAGGTGCGC CTAGTAGATG GGAAAGGCGT CCCTATACCA
AATAAAGTCA TATTCATCAG AGGAAATGAA GCAAACTATT ACTCCAATGC TACCACGGAT
GAGCATGGCC TTGTACAGTT CTCTATCAAC ACCACCAATG TTATGGGTAC CTCTCTTACT
GTTAGGGTCA ATTACAAGGA TCGTAGTCCC TGTTACGGCT ACCAGTGGGT GTCAGAAGAA
CACGAAGAGG CACATCACAC TGCTTATCTT GTGTTCTCCC CAAGCAAGAG CTTTGTCCAC
CTTGAGCCCA TGTCTCATGA ACTACCCTGT GGCCATACTC AGACAGTCCA GGCACATTAT
ATTCTGAATG GAGGCACCCT GCTGGGGCTG AAGAAGCTCT CCTTCTATTA TCTGATAATG
GCAAAGGGAG GCATTGTCCG AACTGGGACT CATGGACTGC TTGTGAAGCA GGAAGACATG
AAGGGCCATT TTTCCATCTC AATCCCTGTG AAGTCAGACA TTGCTCCTGT CGCTCGGTTG
CTCATCTATG CTGTTTTACC TACCGGGGAC GTGATTGGGG ATTCTGCAAA ATATGATGTT
GAAAATTGTC TGGCCAACAA GGTGGATTTG AGCTTCAGCC CATCACAAAG TCTCCCAGCC
TCACACGCCC ACCTGCGAGT CACAGCGGCT CCTCAGTCCG TCTGCGCCCT CCGTGCTGTG
GACCAAAGCG TGCTGCTCAT GAAGCCTGAT GCTGAGCTCT CGGCGTCCTC GGTTTACAAC
CTGCTACCAG AAAAGGACCT CACTGGCTTC CCTGGGCCTT TGAATGACCA GGACGATGAA
GACTGCATCA ATCGTCATAA TGTCTATATT AATGGAATCA CATATACTCC AGTATCAAGT
ACAAATGAAA AGGATATGTA CAGCTTCCTA GAGGACATGG GCTTAAAGGC ATTCACCAAC
TCAAAGATTC GTAAACCCAA AATGTGTCCA CAGCTTCAAC AGTATGAAAT GCATGGACCT
GAAGGTCTAC GTGTAGGTTT TTATGAGTCA GATGTAATGG GAAGAGGCCA TGCACGCCTG
GTGCATGTTG AAGAGCCTCA CACGGAGACC GTACGAAAGT ACTTCCCTGA GACATGGATC
TGGGATTTGG TGGTGGTAAA CTCAGCAGGT GTGGCTGAGG TAGGAGTAAC AGTCCCTGAC
ACCATCACCG AGTGGAAGGC AGGGGCCTTC TGCCTGTCTG AAGATGCTGG ACTTGGTATC
TCTTCCACTG CCTCTCTCCG AGCCTTCCAG CCCTTCTTTG TGGAGCTCAC AATGCCTTAC
TCTGTGATTC GTGGAGAGGC CTTCACACTC AAGGCCACGG TCCTAAACTA CCTTCCCAAA
TGCATCCGGG TCAGTGTGCA GCTGGAAGCC TCTCCCGCCT TCCTAGCTGT CCCAGTGGAG
AAGGAACAAG CGCCTCACTG CATCTGTGCA AACGGGCGGC AAACTGTGTC CTGGGCAGTA
ACCCCAAAGT CATTAGGAAA TGTGAATTTC ACTGTGAGCG CAGAGGCACT AGAGTCTCAA
GAGCTGTGTG GGACTGAGGT GCCTTCAGTT CCTGAACACG GAAGGAAAGA CACAGTCATC
AAGCCTCTGT TGGTTGAACC TGAAGGACTA GAGAAGGAAA CAACATTCAA CTCCCTACTT
TGTCCATCAG GTGGTGAGGT TTCTGAAGAA TTATCCCTGA AACTGCCACC AAATGTGGTA
GAAGAATCTG CCCGAGCTTC TGTCTCAGTT TTGGGAGACA TATTAGGCTC TGCCATGCAA
AACACACAAA ATCTTCTCCA GATGCCCTAT GGCTGTGGAG AGCAGAATAT GGTCCTCTTT
GCTCCTAACA TCTATGTACT GGATTATCTA AATGAAACAC AGCAGCTTAC TCCAGAGATC
AAGTCCAAGG CCATTGGCTA TCTCAACACT GGTTACCAGA GACAGTTGAA CTACAAACAC
TATGATGGCT CCTACAGCAC CTTTGGGGAG CGATATGGCA GGAACCAGGG CAACACCTGG
CTCACAGCCT TTGTTCTGAA GACTTTTGCC CAAGCTCGAG CCTACATCTT CATCGATGAA
GCACACATTA CCCAAGCCCT CATATGGCTC TCCCAGAGGC AGAAGGACAA TGGCTGTTTC
AGGAGCTCTG GGTCACTGCT CAACAATGCC ATAAAGGGAG GAGTAGAAGA TGAAGTGACC
CTCTCCGCCT ATATCACCAT CGCCCTTCTG GAGATTCCTC TCACAGTCAC TCACCCTGTT
GTCCGCAATG CCCTGTTTTG CCTGGAGTCA GCCTGGAAGA CAGCACAAGA AGGGGACCAT
GGCAGCCATG TATATACCAA AGCACTGCTG GCCTATGCTT TTGCCCTGGC AGGTAACCAG
GACAAGAGGA AGGAAGTACT CAAGTCACTT AATGAGGAAG CTGTGAAGAA AGACAACTCT
GTCCATTGGG AGCGCCCTCA GAAACCCAAG GCACCAGTGG GGCATTTTTA CGAACCCCAG
GCTCCCTCTG CTGAGGTGGA GATGACATCC TATGTGCTCC TCGCTTATCT CACGGCCCAG
CCAGCCCCAA CCTCGGAGGA CCTGACCTCT GCAACCAACA TCGTGAAGTG GATCACGAAG
CAGCAGAATG CCCAGGGCGG TTTCTCCTCC ACCCAGGACA CAGTGGTGGC TCTCCATGCT
CTGTCCAAAT ATGGAGCAGC CACATTTACC AGGACTGGGA AGGCTGCACA GGTGACTATC
CAGTCTTCAG GGACATTTTC CAGCAAATTC CAAGTGGACA ACAACAACCG CCTGTTACTG
CAGCAGGTCT CATTGCCAGA GCTGCCTGGG GAATACAGCA TGAAAGTGAC AGGAGAAGGA
TGTGTCTACC TCCAGACATC CTTGAAATAC AATATTCTCC CAGAAAAGGA AGAGTTCCCC
TTTGCTTTAG GAGTGCAGAC TCTGCCTCAA ACTTGTGATG AACCCAAAGC CCACACCAGC
TTCCAAATCT CCCTAAGTGT CAGTTACACA GGGAGCCGCT CTGCCTCCAA CATGGCGATC
GTTGATGTGA AGATGGTCTC TGGCTTCATT CCCCTGAAGC CAACAGTGAA AATGCTTGAA
AGATCTAACC ATGTGAGCCG GACAGAAGTC AGCAGCAACC ATGTCTTGAT TTACCTTGAT
AAGGTGTCAA ATCAGACACT GAGCTTGTTC TTCACGGTTC TGCAAGATGT CCCAGTAAGA
GATCTGAAAC CAGCCATAGT GAAAGTCTAT GATTACTACG AGACGGATGA GTTTGCAATT
GCTGAGTACA ATGCTCCTTG CAGCAAAGAT CTTGGAAATG CTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000005.2
CDS381..4805
Misc Feature(1)327..329
Misc Feature(2)<750..>3593
Misc Feature(3)765..1043
Misc Feature(4)1761..2198
Misc Feature(5)2448..2564
Misc Feature(6)2484..2486
Misc Feature(7)2490..2507
Misc Feature(8)2535..2549
Misc Feature(9)2535..2537
Misc Feature(10)2568..2585
Misc Feature(11)2592..2864
Misc Feature(12)2880..2882
Misc Feature(13)2985..2987
Misc Feature(14)3252..4172
Misc Feature(15)order(3294..3296,3300..3305,3312..3314,3432..3434,3462..3464,3471..3473,3633..3635,3639..3644,4122..4124)
Misc Feature(16)3294..3305
Misc Feature(17)3351..3353
Misc Feature(18)3408..4178
Misc Feature(19)order(3600..3602,3612..3614,3618..3620,3642..3644)
Misc Feature(20)4506..4769
Misc Feature(21)4650..4652
Exon (1)1..466
Exon (2)467..650
Exon (3)651..810
Exon (4)811..863
Exon (5)864..884
Exon (6)885..1053
Exon (7)1054..1138
Exon (8)1139..1259
Exon (9)1260..1374
Exon (10)1375..1484
Exon (11)1485..1646
Exon (12)1647..1874
Exon (13)1875..1938
Exon (14)1939..2081
Exon (15)2082..2231
Exon (16)2232..2393
Exon (17)2394..2505
Exon (18)2506..2620
Exon (19)2621..2849
Exon (20)2850..2976
Exon (21)2977..3098
Exon (22)3099..3150
Exon (23)3151..3234
Exon (24)3235..3411
Exon (25)3412..3499
Exon (26)3500..3656
Exon (27)3657..3731
Exon (28)3732..3912
Exon (29)3913..4136
Exon (30)4137..4355
Exon (31)4356..4483
Exon (32)4484..4574
Exon (33)4575..4643
Exon (34)4644..4746
Exon (35)4747..4788
Exon (36)4789..4920
Translation

Target ORF information:

RefSeq Version NM_000014.5
Organism Homo sapiens(Human)
Definition Homo sapiens alpha-2-macroglobulin (A2M), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000014.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGGGGAAGA ACAAACTCCT TCATCCAAGT CTGGTTCTTC TCCTCTTGGT CCTCCTGCCC 
ACAGACGCCT CAGTCTCTGG AAAACCGCAG TATATGGTTC TGGTCCCCTC CCTGCTCCAC
ACTGAGACCA CTGAGAAGGG CTGTGTCCTT CTGAGCTACC TGAATGAGAC AGTGACTGTA
AGTGCTTCCT TGGAGTCTGT CAGGGGAAAC AGGAGCCTCT TCACTGACCT GGAGGCGGAG
AATGACGTAC TCCACTGTGT CGCCTTCGCT GTCCCAAAGT CTTCATCCAA TGAGGAGGTA
ATGTTCCTCA CTGTCCAAGT GAAAGGACCA ACCCAAGAAT TTAAGAAGCG GACCACAGTG
ATGGTTAAGA ACGAGGACAG TCTGGTCTTT GTCCAGACAG ACAAATCAAT CTACAAACCA
GGGCAGACAG TGAAATTTCG TGTTGTCTCC ATGGATGAAA ACTTTCACCC CCTGAATGAG
TTGATTCCAC TAGTATACAT TCAGGATCCC AAAGGAAATC GCATCGCACA ATGGCAGAGT
TTCCAGTTAG AGGGTGGCCT CAAGCAATTT TCTTTTCCCC TCTCATCAGA GCCCTTCCAG
GGCTCCTACA AGGTGGTGGT ACAGAAGAAA TCAGGTGGAA GGACAGAGCA CCCTTTCACC
GTGGAGGAAT TTGTTCTTCC CAAGTTTGAA GTACAAGTAA CAGTGCCAAA GATAATCACC
ATCTTGGAAG AAGAGATGAA TGTATCAGTG TGTGGCCTAT ACACATATGG GAAGCCTGTC
CCTGGACATG TGACTGTGAG CATTTGCAGA AAGTATAGTG ACGCTTCCGA CTGCCACGGT
GAAGATTCAC AGGCTTTCTG TGAGAAATTC AGTGGACAGC TAAACAGCCA TGGCTGCTTC
TATCAGCAAG TAAAAACCAA GGTCTTCCAG CTGAAGAGGA AGGAGTATGA AATGAAACTT
CACACTGAGG CCCAGATCCA AGAAGAAGGA ACAGTGGTGG AATTGACTGG AAGGCAGTCC
AGTGAAATCA CAAGAACCAT AACCAAACTC TCATTTGTGA AAGTGGACTC ACACTTTCGA
CAGGGAATTC CCTTCTTTGG GCAGGTGCGC CTAGTAGATG GGAAAGGCGT CCCTATACCA
AATAAAGTCA TATTCATCAG AGGAAATGAA GCAAACTATT ACTCCAATGC TACCACGGAT
GAGCATGGCC TTGTACAGTT CTCTATCAAC ACCACCAATG TTATGGGTAC CTCTCTTACT
GTTAGGGTCA ATTACAAGGA TCGTAGTCCC TGTTACGGCT ACCAGTGGGT GTCAGAAGAA
CACGAAGAGG CACATCACAC TGCTTATCTT GTGTTCTCCC CAAGCAAGAG CTTTGTCCAC
CTTGAGCCCA TGTCTCATGA ACTACCCTGT GGCCATACTC AGACAGTCCA GGCACATTAT
ATTCTGAATG GAGGCACCCT GCTGGGGCTG AAGAAGCTCT CCTTCTATTA TCTGATAATG
GCAAAGGGAG GCATTGTCCG AACTGGGACT CATGGACTGC TTGTGAAGCA GGAAGACATG
AAGGGCCATT TTTCCATCTC AATCCCTGTG AAGTCAGACA TTGCTCCTGT CGCTCGGTTG
CTCATCTATG CTGTTTTACC TACCGGGGAC GTGATTGGGG ATTCTGCAAA ATATGATGTT
GAAAATTGTC TGGCCAACAA GGTGGATTTG AGCTTCAGCC CATCACAAAG TCTCCCAGCC
TCACACGCCC ACCTGCGAGT CACAGCGGCT CCTCAGTCCG TCTGCGCCCT CCGTGCTGTG
GACCAAAGCG TGCTGCTCAT GAAGCCTGAT GCTGAGCTCT CGGCGTCCTC GGTTTACAAC
CTGCTACCAG AAAAGGACCT CACTGGCTTC CCTGGGCCTT TGAATGACCA GGACGATGAA
GACTGCATCA ATCGTCATAA TGTCTATATT AATGGAATCA CATATACTCC AGTATCAAGT
ACAAATGAAA AGGATATGTA CAGCTTCCTA GAGGACATGG GCTTAAAGGC ATTCACCAAC
TCAAAGATTC GTAAACCCAA AATGTGTCCA CAGCTTCAAC AGTATGAAAT GCATGGACCT
GAAGGTCTAC GTGTAGGTTT TTATGAGTCA GATGTAATGG GAAGAGGCCA TGCACGCCTG
GTGCATGTTG AAGAGCCTCA CACGGAGACC GTACGAAAGT ACTTCCCTGA GACATGGATC
TGGGATTTGG TGGTGGTAAA CTCAGCAGGT GTGGCTGAGG TAGGAGTAAC AGTCCCTGAC
ACCATCACCG AGTGGAAGGC AGGGGCCTTC TGCCTGTCTG AAGATGCTGG ACTTGGTATC
TCTTCCACTG CCTCTCTCCG AGCCTTCCAG CCCTTCTTTG TGGAGCTCAC AATGCCTTAC
TCTGTGATTC GTGGAGAGGC CTTCACACTC AAGGCCACGG TCCTAAACTA CCTTCCCAAA
TGCATCCGGG TCAGTGTGCA GCTGGAAGCC TCTCCCGCCT TCCTAGCTGT CCCAGTGGAG
AAGGAACAAG CGCCTCACTG CATCTGTGCA AACGGGCGGC AAACTGTGTC CTGGGCAGTA
ACCCCAAAGT CATTAGGAAA TGTGAATTTC ACTGTGAGCG CAGAGGCACT AGAGTCTCAA
GAGCTGTGTG GGACTGAGGT GCCTTCAGTT CCTGAACACG GAAGGAAAGA CACAGTCATC
AAGCCTCTGT TGGTTGAACC TGAAGGACTA GAGAAGGAAA CAACATTCAA CTCCCTACTT
TGTCCATCAG GTGGTGAGGT TTCTGAAGAA TTATCCCTGA AACTGCCACC AAATGTGGTA
GAAGAATCTG CCCGAGCTTC TGTCTCAGTT TTGGGAGACA TATTAGGCTC TGCCATGCAA
AACACACAAA ATCTTCTCCA GATGCCCTAT GGCTGTGGAG AGCAGAATAT GGTCCTCTTT
GCTCCTAACA TCTATGTACT GGATTATCTA AATGAAACAC AGCAGCTTAC TCCAGAGATC
AAGTCCAAGG CCATTGGCTA TCTCAACACT GGTTACCAGA GACAGTTGAA CTACAAACAC
TATGATGGCT CCTACAGCAC CTTTGGGGAG CGATATGGCA GGAACCAGGG CAACACCTGG
CTCACAGCCT TTGTTCTGAA GACTTTTGCC CAAGCTCGAG CCTACATCTT CATCGATGAA
GCACACATTA CCCAAGCCCT CATATGGCTC TCCCAGAGGC AGAAGGACAA TGGCTGTTTC
AGGAGCTCTG GGTCACTGCT CAACAATGCC ATAAAGGGAG GAGTAGAAGA TGAAGTGACC
CTCTCCGCCT ATATCACCAT CGCCCTTCTG GAGATTCCTC TCACAGTCAC TCACCCTGTT
GTCCGCAATG CCCTGTTTTG CCTGGAGTCA GCCTGGAAGA CAGCACAAGA AGGGGACCAT
GGCAGCCATG TATATACCAA AGCACTGCTG GCCTATGCTT TTGCCCTGGC AGGTAACCAG
GACAAGAGGA AGGAAGTACT CAAGTCACTT AATGAGGAAG CTGTGAAGAA AGACAACTCT
GTCCATTGGG AGCGCCCTCA GAAACCCAAG GCACCAGTGG GGCATTTTTA CGAACCCCAG
GCTCCCTCTG CTGAGGTGGA GATGACATCC TATGTGCTCC TCGCTTATCT CACGGCCCAG
CCAGCCCCAA CCTCGGAGGA CCTGACCTCT GCAACCAACA TCGTGAAGTG GATCACGAAG
CAGCAGAATG CCCAGGGCGG TTTCTCCTCC ACCCAGGACA CAGTGGTGGC TCTCCATGCT
CTGTCCAAAT ATGGAGCAGC CACATTTACC AGGACTGGGA AGGCTGCACA GGTGACTATC
CAGTCTTCAG GGACATTTTC CAGCAAATTC CAAGTGGACA ACAACAACCG CCTGTTACTG
CAGCAGGTCT CATTGCCAGA GCTGCCTGGG GAATACAGCA TGAAAGTGAC AGGAGAAGGA
TGTGTCTACC TCCAGACATC CTTGAAATAC AATATTCTCC CAGAAAAGGA AGAGTTCCCC
TTTGCTTTAG GAGTGCAGAC TCTGCCTCAA ACTTGTGATG AACCCAAAGC CCACACCAGC
TTCCAAATCT CCCTAAGTGT CAGTTACACA GGGAGCCGCT CTGCCTCCAA CATGGCGATC
GTTGATGTGA AGATGGTCTC TGGCTTCATT CCCCTGAAGC CAACAGTGAA AATGCTTGAA
AGATCTAACC ATGTGAGCCG GACAGAAGTC AGCAGCAACC ATGTCTTGAT TTACCTTGAT
AAGGTGTCAA ATCAGACACT GAGCTTGTTC TTCACGGTTC TGCAAGATGT CCCAGTAAGA
GATCTGAAAC CAGCCATAGT GAAAGTCTAT GATTACTACG AGACGGATGA GTTTGCAATT
GCTGAGTACA ATGCTCCTTG CAGCAAAGAT CTTGGAAATG CTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu46081
Clone ID Related Accession (Same CDS sequence) XM_006719056.2
Accession Version XM_006719056.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4539bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product alpha-2-macroglobulin isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006719056.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
ATGGGGAAGA ACAAACTCCT TCATCCAAGT CTGGTTCTTC TCCTCTTGGT CCTCCTGCCC 
ACAGACGCCT CAGTCTCTGG AAAACCGCAG TATATGGTTC TGGTCCCCTC CCTGCTCCAC
ACTGAGACCA CTGAGAAGGG CTGTGTCCTT CTGAGCTACC TGAATGAGAC AGTGACTGTA
AGTGCTTCCT TGGAGTCTGT CAGGGGAAAC AGGAGCCTCT TCACTGACCT GGAGGCGGAG
AATGACGTAC TCCACTGTGT CGCCTTCGCT GTCCCAAAGT CTTCATCCAA TGAGGAGGTA
ATGTTCCTCA CTGTCCAAGT GAAAGGACCA ACCCAAGAAT TTAAGAAGCG GACCACAGTG
ATGGTTAAGA ACGAGGACAG TCTGGTCTTT GTCCAGACAG ACAAATCAAT CTACAAACCA
GGGCAGACAG TGAAATTTCG TGTTGTCTCC ATGGATGAAA ACTTTCACCC CCTGAATGAG
TTGATTCCAC TAGTATACAT TCAGGATCCC AAAGGAAATC GCATCGCACA ATGGCAGAGT
TTCCAGTTAG AGGGTGGCCT CAAGCAATTT TCTTTTCCCC TCTCATCAGA GCCCTTCCAG
GGCTCCTACA AGGTGGTGGT ACAGAAGAAA TCAGGTGGAA GGACAGAGCA CCCTTTCACC
GTGGAGGAAT TTGTTCTTCC CAAGTTTGAA GTACAAGTAA CAGTGCCAAA GATAATCACC
ATCTTGGAAG AAGAGATGAA TGTATCAGTG TGTGGCCTAT ACACATATGG GAAGCCTGTC
CCTGGACATG TGACTGTGAG CATTTGCAGA AAGTATAGTG ACGCTTCCGA CTGCCACGGT
GAAGATTCAC AGGCTTTCTG TGAGAAATTC AGTGGACAGC TAAACAGCCA TGGCTGCTTC
TATCAGCAAG TAAAAACCAA GGTCTTCCAG CTGAAGAGGA AGGAGTATGA AATGAAACTT
CACACTGAGG CCCAGATCCA AGAAGAAGGA ACAGTGGTGG AATTGACTGG AAGGCAGTCC
AGTGAAATCA CAAGAACCAT AACCAAACTC TCATTTGTGA AAGTGGACTC ACACTTTCGA
CAGGGAATTC CCTTCTTTGG GCAGGTGCGC CTAGTAGATG GGAAAGGCGT CCCTATACCA
AATAAAGTCA TATTCATCAG AGGAAATGAA GCAAACTATT ACTCCAATGC TACCACGGAT
GAGCATGGCC TTGTACAGTT CTCTATCAAC ACCACCAATG TTATGGGTAC CTCTCTTACT
GTTAGGGTCA ATTACAAGGA TCGTAGTCCC TGTTACGGCT ACCAGTGGGT GTCAGAAGAA
CACGAAGAGG CACATCACAC TGCTTATCTT GTGTTCTCCC CAAGCAAGAG CTTTGTCCAC
CTTGAGCCCA TGTCTCATGA ACTACCCTGT GGCCATACTC AGACAGTCCA GGCACATTAT
ATTCTGAATG GAGGCACCCT GCTGGGGCTG AAGAAGCTCT CCTTCTATTA TCTGATAATG
GCAAAGGGAG GCATTGTCCG AACTGGGACT CATGGACTGC TTGTGAAGCA GGAAGACATG
AAGGGCCATT TTTCCATCTC AATCCCTGTG AAGTCAGACA TTGCTCCTGT CGCTCGGTTG
CTCATCTATG CTGTTTTACC TACCGGGGAC GTGATTGGGG ATTCTGCAAA ATATGATGTT
GAAAATTGTC TGGCCAACAA GGTGGATTTG AGCTTCAGCC CATCACAAAG TCTCCCAGCC
TCACACGCCC ACCTGCGAGT CACAGCGGCT CCTCAGTCCG TCTGCGCCCT CCGTGCTGTG
GACCAAAGCG TGCTGCTCAT GAAGCCTGAT GCTGAGCTCT CGGCGTCCTC GGTTTACAAC
CTGCTACCAG AAAAGGACCT CACTGGCTTC CCTGGGCCTT TGAATGACCA GGACAATGAA
GACTGCATCA ATCGTCATAA TGTCTATATT AATGGAATCA CATATACTCC AGTATCAAGT
ACAAATGAAA AGGATATGTA CAGCTTCCTA GAGGACATGG GCTTAAAGGC ATTCACCAAC
TCAAAGATTC GTAAACCCAA AATGTGTCCA CAGCTTCAAC AGTATGAAAT GCATGGACCT
GAAGGTCTAC GTGTAGGTTT TTATGAGTCA GATGTAATGG GAAGAGGCCA TGCACGCCTG
GTGCATGTTG AAGAGCCTCA CACGGAGACC GTACGAAAGT ACTTCCCTGA GACATGGATC
TGGGATTTGG TGGTGGTAAA CTCAGCAGGT GTGGCTGAGG TAGGAGTAAC AGTCCCTGAC
ACCATCACCG AGTGGAAGGC AGGGGCCTTC TGCCTGTCTG AAGATGCTGG ACTTGGTATC
TCTTCCACTG CCTCTCTCCG AGCCTTCCAG CCCTTCTTTG TGGAGCTCAC AATGCCTTAC
TCTGTGATTC GTGGAGAGGC CTTCACACTC AAGGCCACGG TCCTAAACTA CCTTCCCAAA
TGCATCCGGG TCAGTGTGCA GCTGGAAGCC TCTCCCGCCT TCCTAGCTGT CCCAGTGGAG
AAGGAACAAG CGCCTCACTG CATCTGTGCA AACGGGCGGC AAACTGTGTC CTGGGCAGTA
ACCCCAAAGT CATTAGGAAA TGTGAATTTC ACTGTGAGCG CAGAGGCACT AGAGTCTCAA
GAGCTGTGTG GGACTGAGGT GCCTTCAGTT CCTGAACACG GAAGGAAAGA CACAGTCATC
AAGCCTCTGT TGGTTGAACC TGAAGGACTA GAGAAGGAAA CAACATTCAA CTCCCTACTT
TGTCCATCAG GTGGTGAGGT TTCTGAAGAA TTATCCCTGA AACTGCCACC AAATGTGGTA
GAAGAATCTG CCCGAGCTTC TGTCTCAGTT TTGGGAGACA TATTAGGCTC TGCCATGCAA
AACACACAAA ATCTTCTCCA GATGCCCTAT GGCTGTGGAG AGCAGAATAT GGTCCTCTTT
GCTCCTAACA TCTATGTACT GGATTATCTA AATGAAACAC AGCAGCTTAC TCCAGAGATC
AAGTCCAAGG CCATTGGCTA TCTCAACACT GGTTACCAGA GACAGTTGAA CTACAAACAC
TATGATGGCT CCTACAGCAC CTTTGGGGAG CGATATGGCA GGAACCAGGG CAACACCTGG
CTCACAGCCT TTGTTCTGAA GACTTTTGCC CAAGCTCGAG CCTACATCTT CATCGATGAA
GCACACATTA CCCAAGCCCT CATATGGCTC TCCCAGAGGC AGAAGGACAA TGGCTGTTTC
AGGAGCTCTG GGTCACTGCT CAACAATGCC ATAAAGGGAG GAGTAGAAGA TGAAGTGACC
CTCTCCGCCT ATATCACCAT CGCCCTTCTG GAGATTCCTC TCACAGTCAC TCACCCTGTT
GTCCGCAATG CCCTGTTTTG CCTGGAGTCA GCCTGGAAGA CAGCACAAGA AGGGGACCAT
GGCAGCCATG TATATACCAA AGCACTGCTG GCCTATGCTT TTGCCCTGGC AGGTAACCAG
GACAAGAGGA AGGAAGTACT CAAGTCACTT AATGAGGAAG CTGTGAAGAA AGACAACTCT
GTCCATTGGG AGCGCCCTCA GAAACCCAAG GCACCAGTGG GGCATTTTTA CGAACCCCAG
GCTCCCTCTG CTGAGGTGGA GATGACATCC TATGTGCTCC TCGCTTATCT CACGGCCCAG
CCAGCCCCAA CCTCGGAGGA CCTGACCTCT GCAACCAACA TCGTGAAGTG GATCACGAAG
CAGCAGAATG CCCAGGGCGG TTTCTCCTCC ACCCAGGACA CAGTGGTGGC TCTCCATGCT
CTGTCCAAAT ATGGAGCAGC CACATTTACC AGGACTGGGA AGGCTGCACA GGTGACTATC
CAGTCTTCAG GGACATTTTC CAGCAAATTC CAAGTGGACA ACAACAACCG CCTGTTACTG
CAGCAGGTCT CATTGCCAGA GCTGCCTGGG GAATACAGCA TGAAAGTGAC AGGAGAAGGA
TGTGTCTACC TCCAGACATC CTTGAAATAC AATATTCTCC CAGAAAAGGA AGAGTTCCCC
TTTGCTTTAG GAGTGCAGAC TCTGCCTCAA ACTTGTGATG AACCCAAAGC CCACACCAGC
TTCCAAATCT CCCTAAGTGT CAGTTACACA GGGAGCCGCT CTGCCTCCAA CATGGCGATC
GTTGATGTGA AGATGGTCTC TGGCTTCATT CCCCTGAAGC CAACAGTGAA AATGCTTGAA
AGATCTAACC ATGTGAGCCG GACAGAAGTC AGCAGCAACC ATGTCTTGAT TTACCTTGAT
AAGGTGTCAA ATCAGACACT GAGCTTGTTC TTCACGGTTC TGCAAGATGT CCCAGTAAGA
GATCTGAAAC CAGCCATAGT GAAAGTCTAT GATTACTACG AGACGGATGA GTTTGCAATT
GCTGAGTACA ATGCTCCTTG CAGCAAAGGT AAGCCACTCA CACTCCTCCA AAAGGCAGTC
AGAGCTCCTT CAGCTTGCCC CCCAAACCTT CTCCTTCATA AAACGCTGGG TAAATATTTG
TCAAAAACAT CAAATTACTC ACACTGCACA TTATTATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006719119.1
CDS92..4630
Misc Feature(1)<461..>3304
Misc Feature(2)476..754
Misc Feature(3)1472..1909
Misc Feature(4)2303..2575
Misc Feature(5)2963..3883
Misc Feature(6)order(3005..3007,3011..3016,3023..3025,3143..3145,3173..3175,3182..3184,3344..3346,3350..3355,3833..3835)
Misc Feature(7)3005..3016
Misc Feature(8)3119..3889
Misc Feature(9)order(3311..3313,3323..3325,3329..3331,3353..3355)
Misc Feature(10)4217..4480
Translation

Target ORF information:

RefSeq Version XM_006719056.2
Organism Homo sapiens(Human)
Definition Homo sapiens alpha-2-macroglobulin (A2M), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006719056.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
ATGGGGAAGA ACAAACTCCT TCATCCAAGT CTGGTTCTTC TCCTCTTGGT CCTCCTGCCC 
ACAGACGCCT CAGTCTCTGG AAAACCGCAG TATATGGTTC TGGTCCCCTC CCTGCTCCAC
ACTGAGACCA CTGAGAAGGG CTGTGTCCTT CTGAGCTACC TGAATGAGAC AGTGACTGTA
AGTGCTTCCT TGGAGTCTGT CAGGGGAAAC AGGAGCCTCT TCACTGACCT GGAGGCGGAG
AATGACGTAC TCCACTGTGT CGCCTTCGCT GTCCCAAAGT CTTCATCCAA TGAGGAGGTA
ATGTTCCTCA CTGTCCAAGT GAAAGGACCA ACCCAAGAAT TTAAGAAGCG GACCACAGTG
ATGGTTAAGA ACGAGGACAG TCTGGTCTTT GTCCAGACAG ACAAATCAAT CTACAAACCA
GGGCAGACAG TGAAATTTCG TGTTGTCTCC ATGGATGAAA ACTTTCACCC CCTGAATGAG
TTGATTCCAC TAGTATACAT TCAGGATCCC AAAGGAAATC GCATCGCACA ATGGCAGAGT
TTCCAGTTAG AGGGTGGCCT CAAGCAATTT TCTTTTCCCC TCTCATCAGA GCCCTTCCAG
GGCTCCTACA AGGTGGTGGT ACAGAAGAAA TCAGGTGGAA GGACAGAGCA CCCTTTCACC
GTGGAGGAAT TTGTTCTTCC CAAGTTTGAA GTACAAGTAA CAGTGCCAAA GATAATCACC
ATCTTGGAAG AAGAGATGAA TGTATCAGTG TGTGGCCTAT ACACATATGG GAAGCCTGTC
CCTGGACATG TGACTGTGAG CATTTGCAGA AAGTATAGTG ACGCTTCCGA CTGCCACGGT
GAAGATTCAC AGGCTTTCTG TGAGAAATTC AGTGGACAGC TAAACAGCCA TGGCTGCTTC
TATCAGCAAG TAAAAACCAA GGTCTTCCAG CTGAAGAGGA AGGAGTATGA AATGAAACTT
CACACTGAGG CCCAGATCCA AGAAGAAGGA ACAGTGGTGG AATTGACTGG AAGGCAGTCC
AGTGAAATCA CAAGAACCAT AACCAAACTC TCATTTGTGA AAGTGGACTC ACACTTTCGA
CAGGGAATTC CCTTCTTTGG GCAGGTGCGC CTAGTAGATG GGAAAGGCGT CCCTATACCA
AATAAAGTCA TATTCATCAG AGGAAATGAA GCAAACTATT ACTCCAATGC TACCACGGAT
GAGCATGGCC TTGTACAGTT CTCTATCAAC ACCACCAATG TTATGGGTAC CTCTCTTACT
GTTAGGGTCA ATTACAAGGA TCGTAGTCCC TGTTACGGCT ACCAGTGGGT GTCAGAAGAA
CACGAAGAGG CACATCACAC TGCTTATCTT GTGTTCTCCC CAAGCAAGAG CTTTGTCCAC
CTTGAGCCCA TGTCTCATGA ACTACCCTGT GGCCATACTC AGACAGTCCA GGCACATTAT
ATTCTGAATG GAGGCACCCT GCTGGGGCTG AAGAAGCTCT CCTTCTATTA TCTGATAATG
GCAAAGGGAG GCATTGTCCG AACTGGGACT CATGGACTGC TTGTGAAGCA GGAAGACATG
AAGGGCCATT TTTCCATCTC AATCCCTGTG AAGTCAGACA TTGCTCCTGT CGCTCGGTTG
CTCATCTATG CTGTTTTACC TACCGGGGAC GTGATTGGGG ATTCTGCAAA ATATGATGTT
GAAAATTGTC TGGCCAACAA GGTGGATTTG AGCTTCAGCC CATCACAAAG TCTCCCAGCC
TCACACGCCC ACCTGCGAGT CACAGCGGCT CCTCAGTCCG TCTGCGCCCT CCGTGCTGTG
GACCAAAGCG TGCTGCTCAT GAAGCCTGAT GCTGAGCTCT CGGCGTCCTC GGTTTACAAC
CTGCTACCAG AAAAGGACCT CACTGGCTTC CCTGGGCCTT TGAATGACCA GGACAATGAA
GACTGCATCA ATCGTCATAA TGTCTATATT AATGGAATCA CATATACTCC AGTATCAAGT
ACAAATGAAA AGGATATGTA CAGCTTCCTA GAGGACATGG GCTTAAAGGC ATTCACCAAC
TCAAAGATTC GTAAACCCAA AATGTGTCCA CAGCTTCAAC AGTATGAAAT GCATGGACCT
GAAGGTCTAC GTGTAGGTTT TTATGAGTCA GATGTAATGG GAAGAGGCCA TGCACGCCTG
GTGCATGTTG AAGAGCCTCA CACGGAGACC GTACGAAAGT ACTTCCCTGA GACATGGATC
TGGGATTTGG TGGTGGTAAA CTCAGCAGGT GTGGCTGAGG TAGGAGTAAC AGTCCCTGAC
ACCATCACCG AGTGGAAGGC AGGGGCCTTC TGCCTGTCTG AAGATGCTGG ACTTGGTATC
TCTTCCACTG CCTCTCTCCG AGCCTTCCAG CCCTTCTTTG TGGAGCTCAC AATGCCTTAC
TCTGTGATTC GTGGAGAGGC CTTCACACTC AAGGCCACGG TCCTAAACTA CCTTCCCAAA
TGCATCCGGG TCAGTGTGCA GCTGGAAGCC TCTCCCGCCT TCCTAGCTGT CCCAGTGGAG
AAGGAACAAG CGCCTCACTG CATCTGTGCA AACGGGCGGC AAACTGTGTC CTGGGCAGTA
ACCCCAAAGT CATTAGGAAA TGTGAATTTC ACTGTGAGCG CAGAGGCACT AGAGTCTCAA
GAGCTGTGTG GGACTGAGGT GCCTTCAGTT CCTGAACACG GAAGGAAAGA CACAGTCATC
AAGCCTCTGT TGGTTGAACC TGAAGGACTA GAGAAGGAAA CAACATTCAA CTCCCTACTT
TGTCCATCAG GTGGTGAGGT TTCTGAAGAA TTATCCCTGA AACTGCCACC AAATGTGGTA
GAAGAATCTG CCCGAGCTTC TGTCTCAGTT TTGGGAGACA TATTAGGCTC TGCCATGCAA
AACACACAAA ATCTTCTCCA GATGCCCTAT GGCTGTGGAG AGCAGAATAT GGTCCTCTTT
GCTCCTAACA TCTATGTACT GGATTATCTA AATGAAACAC AGCAGCTTAC TCCAGAGATC
AAGTCCAAGG CCATTGGCTA TCTCAACACT GGTTACCAGA GACAGTTGAA CTACAAACAC
TATGATGGCT CCTACAGCAC CTTTGGGGAG CGATATGGCA GGAACCAGGG CAACACCTGG
CTCACAGCCT TTGTTCTGAA GACTTTTGCC CAAGCTCGAG CCTACATCTT CATCGATGAA
GCACACATTA CCCAAGCCCT CATATGGCTC TCCCAGAGGC AGAAGGACAA TGGCTGTTTC
AGGAGCTCTG GGTCACTGCT CAACAATGCC ATAAAGGGAG GAGTAGAAGA TGAAGTGACC
CTCTCCGCCT ATATCACCAT CGCCCTTCTG GAGATTCCTC TCACAGTCAC TCACCCTGTT
GTCCGCAATG CCCTGTTTTG CCTGGAGTCA GCCTGGAAGA CAGCACAAGA AGGGGACCAT
GGCAGCCATG TATATACCAA AGCACTGCTG GCCTATGCTT TTGCCCTGGC AGGTAACCAG
GACAAGAGGA AGGAAGTACT CAAGTCACTT AATGAGGAAG CTGTGAAGAA AGACAACTCT
GTCCATTGGG AGCGCCCTCA GAAACCCAAG GCACCAGTGG GGCATTTTTA CGAACCCCAG
GCTCCCTCTG CTGAGGTGGA GATGACATCC TATGTGCTCC TCGCTTATCT CACGGCCCAG
CCAGCCCCAA CCTCGGAGGA CCTGACCTCT GCAACCAACA TCGTGAAGTG GATCACGAAG
CAGCAGAATG CCCAGGGCGG TTTCTCCTCC ACCCAGGACA CAGTGGTGGC TCTCCATGCT
CTGTCCAAAT ATGGAGCAGC CACATTTACC AGGACTGGGA AGGCTGCACA GGTGACTATC
CAGTCTTCAG GGACATTTTC CAGCAAATTC CAAGTGGACA ACAACAACCG CCTGTTACTG
CAGCAGGTCT CATTGCCAGA GCTGCCTGGG GAATACAGCA TGAAAGTGAC AGGAGAAGGA
TGTGTCTACC TCCAGACATC CTTGAAATAC AATATTCTCC CAGAAAAGGA AGAGTTCCCC
TTTGCTTTAG GAGTGCAGAC TCTGCCTCAA ACTTGTGATG AACCCAAAGC CCACACCAGC
TTCCAAATCT CCCTAAGTGT CAGTTACACA GGGAGCCGCT CTGCCTCCAA CATGGCGATC
GTTGATGTGA AGATGGTCTC TGGCTTCATT CCCCTGAAGC CAACAGTGAA AATGCTTGAA
AGATCTAACC ATGTGAGCCG GACAGAAGTC AGCAGCAACC ATGTCTTGAT TTACCTTGAT
AAGGTGTCAA ATCAGACACT GAGCTTGTTC TTCACGGTTC TGCAAGATGT CCCAGTAAGA
GATCTGAAAC CAGCCATAGT GAAAGTCTAT GATTACTACG AGACGGATGA GTTTGCAATT
GCTGAGTACA ATGCTCCTTG CAGCAAAGGT AAGCCACTCA CACTCCTCCA AAAGGCAGTC
AGAGCTCCTT CAGCTTGCCC CCCAAACCTT CTCCTTCATA AAACGCTGGG TAAATATTTG
TCAAAAACAT CAAATTACTC ACACTGCACA TTATTATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

HK2 Proximal Tubule Epithelial Cells Synthesize and Secrete Plasma Proteins Predominantly Through the Apical Surface.
Journal of cellular biochemistry118(4)924-933(2017 Apr)
Zhao KW,Murray EJ,Murray SS


book

Inactivation of viruses by pasteurization at 60 °C for 10 h with and without 40% glucose as stabilizer during a new manufacturing process of α2-Macroglobulin from Cohn Fraction IV.
Biologicals : journal of the International Association of Biological Standardization46139-142(2017 Mar)
Huangfu C,Ma Y,Jia J,Lv M,Zhu F,Ma X,Zhao X,Zhang J


book

Alpha-2 macroglobulin in Alzheimer's disease: a marker of neuronal injury through the RCAN1 pathway.
Molecular psychiatry22(1)13-23(2017 Jan)
Varma VR,Varma S,An Y,Hohman TJ,Seddighi S,Casanova R,Beri A,Dammer EB,Seyfried NT,Pletnikova O,Moghekar A,Wilson MR,Lah JJ,O'Brien RJ,Levey AI,Troncoso JC,Albert MS,Thambisetty M


book

Mutations in CPAMD8 Cause a Unique Form of Autosomal-Recessive Anterior Segment Dysgenesis.
American journal of human genetics99(6)1338-1352(2016 Dec)
Cheong SS,Hentschel L,Davidson AE,Gerrelli D,Davie R,Rizzo R,Pontikos N,Plagnol V,Moore AT,Sowden JC,Michaelides M,Snead M,Tuft SJ,Hardcastle AJ


book

Primary structure of human alpha 2-macroglobulin. Complete disulfide bridge assignment and localization of two interchain bridges in the dimeric proteinase binding unit.
The Journal of biological chemistry261(34)15863-9(1986 Dec)
Jensen PE,Sottrup-Jensen L


 
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