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F7 cDNA ORF clone, Homo sapiens (human)

Gene Symbol F7
Entrez Gene ID 2155
Full Name coagulation factor VII (serum prothrombin conversion accelerator)
Synonyms SPCA
General protein information
Preferred Names
coagulation factor VII
Names
coagulation factor VII
eptacog alfa
proconvertin
FVII coagulation protein
NP_000122.1
NP_001254483.1
NP_062562.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

13

13q34

Summary This gene encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Defects in this gene can cause coagulopathy. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides. [provided by RefSeq, Aug 2015]. lac of sum
Disorder MIM:

613878


mRNA and Protein(s)

mRNA Protein Name
NM_000131 NP_000122 coagulation factor VII isoform a preproprotein
NM_019616 NP_062562 coagulation factor VII isoform b precursor
NM_001267554 NP_001254483 coagulation factor VII isoform c precursor
XM_011537474 XP_011535776 coagulation factor VII isoform X1
XM_011537475 XP_011535777 coagulation factor VII isoform X2
XM_006719963 XP_006720026 coagulation factor VII isoform X3
XM_011537476 XP_011535778 coagulation factor VII isoform X4
XM_011537477 XP_011535779 coagulation factor VII isoform X5



Homo sapiens (human) F7 NP_062562.1
Pan troglodytes (chimpanzee) F7 XP_001149885.2
Macaca mulatta (Rhesus monkey) F7 NP_001073605.1
Canis lupus familiaris (dog) F7 NP_001041498.1
Bos taurus (cattle) F7 NP_001029978.1
Mus musculus (house mouse) F7 NP_034302.2
Rattus norvegicus (Norway rat) F7 NP_690059.1
Gallus gallus (chicken) F7 NP_989773.1
Danio rerio (zebrafish) zgc:163025 NP_001083027.1
Xenopus (Silurana) tropicalis (western clawed frog) f7 NP_001072819.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following F7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu25117
NM_000131 Homo sapiens coagulation factor VII (serum prothrombin conversion accelerator) (F7), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$194.50-$272.30
$389.00
OHu25271
NM_019616 Homo sapiens coagulation factor VII (serum prothrombin conversion accelerator) (F7), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu25174 NM_001267554 Homo sapiens coagulation factor VII (serum prothrombin conversion accelerator) (F7), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu57662 XM_011537474 PREDICTED: Homo sapiens coagulation factor VII (serum prothrombin conversion accelerator) (F7), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu57663 XM_011537475 PREDICTED: Homo sapiens coagulation factor VII (serum prothrombin conversion accelerator) (F7), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu47412 XM_006719963 PREDICTED: Homo sapiens coagulation factor VII (serum prothrombin conversion accelerator) (F7), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu57664 XM_011537476 PREDICTED: Homo sapiens coagulation factor VII (serum prothrombin conversion accelerator) (F7), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu57665 XM_011537477 PREDICTED: Homo sapiens coagulation factor VII (serum prothrombin conversion accelerator) (F7), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu25117
Clone ID Related Accession (Same CDS sequence) NM_000131
Accession Version NM_000131.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1401bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 16-AUG-2015
Organism Homo sapiens (human)
Product coagulation factor VII isoform a preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK310779.1, M13232.1, AL137002.19 and AI076552.1. On May 31, 2012 this sequence version replaced gi:116805320. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. A downstream translational start codon is selected for this RefSeq based on its better conservation in mammalian species. An upstream in-frame start codon is also present and would result in a protein that is 15 aa longer at the N-terminus, but this start codon is poorly conserved and located within a protein-binding site identified in the promoter region, as described in PubMed: 8576177. Leaky scanning by ribosomes may allow translation initiation at the downstream start codon. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:8576177) ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: M13232.1, EU557239.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2155590 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTCTCCC AGGCCCTCAG GCTCCTCTGC CTTCTGCTTG GGCTTCAGGG CTGCCTGGCT 
GCAGGCGGGG TCGCTAAGGC CTCAGGAGGA GAAACACGGG ACATGCCGTG GAAGCCGGGG
CCTCACAGAG TCTTCGTAAC CCAGGAGGAA GCCCACGGCG TCCTGCACCG GCGCCGGCGC
GCCAACGCGT TCCTGGAGGA GCTGCGGCCG GGCTCCCTGG AGAGGGAGTG CAAGGAGGAG
CAGTGCTCCT TCGAGGAGGC CCGGGAGATC TTCAAGGACG CGGAGAGGAC GAAGCTGTTC
TGGATTTCTT ACAGTGATGG GGACCAGTGT GCCTCAAGTC CATGCCAGAA TGGGGGCTCC
TGCAAGGACC AGCTCCAGTC CTATATCTGC TTCTGCCTCC CTGCCTTCGA GGGCCGGAAC
TGTGAGACGC ACAAGGATGA CCAGCTGATC TGTGTGAACG AGAACGGCGG CTGTGAGCAG
TACTGCAGTG ACCACACGGG CACCAAGCGC TCCTGTCGGT GCCACGAGGG GTACTCTCTG
CTGGCAGACG GGGTGTCCTG CACACCCACA GTTGAATATC CATGTGGAAA AATACCTATT
CTAGAAAAAA GAAATGCCAG CAAACCCCAA GGCCGAATTG TGGGGGGCAA GGTGTGCCCC
AAAGGGGAGT GTCCATGGCA GGTCCTGTTG TTGGTGAATG GAGCTCAGTT GTGTGGGGGG
ACCCTGATCA ACACCATCTG GGTGGTCTCC GCGGCCCACT GTTTCGACAA AATCAAGAAC
TGGAGGAACC TGATCGCGGT GCTGGGCGAG CACGACCTCA GCGAGCACGA CGGGGATGAG
CAGAGCCGGC GGGTGGCGCA GGTCATCATC CCCAGCACGT ACGTCCCGGG CACCACCAAC
CACGACATCG CGCTGCTCCG CCTGCACCAG CCCGTGGTCC TCACTGACCA TGTGGTGCCC
CTCTGCCTGC CCGAACGGAC GTTCTCTGAG AGGACGCTGG CCTTCGTGCG CTTCTCATTG
GTCAGCGGCT GGGGCCAGCT GCTGGACCGT GGCGCCACGG CCCTGGAGCT CATGGTCCTC
AACGTGCCCC GGCTGATGAC CCAGGACTGC CTGCAGCAGT CACGGAAGGT GGGAGACTCC
CCAAATATCA CGGAGTACAT GTTCTGTGCC GGCTACTCGG ATGGCAGCAA GGACTCCTGC
AAGGGGGACA GTGGAGGCCC ACATGCCACC CACTACCGGG GCACGTGGTA CCTGACGGGC
ATCGTCAGCT GGGGCCAGGG CTGCGCAACC GTGGGCCACT TTGGGGTGTA CACCAGGGTC
TCCCAGTACA TCGAGTGGCT GCAAAAGCTC ATGCGCTCAG AGCCACGCCC AGGAGTCCTC
CTGCGAGCCC CATTTCCCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000122.1
CDS55..1455
Misc Feature(1)178..366(+)
Misc Feature(2)250..252(+)
Misc Feature(3)253..255(+)
Misc Feature(4)274..276(+)
Misc Feature(5)280..282(+)
Misc Feature(6)289..291(+)
Misc Feature(7)292..294(+)
Misc Feature(8)307..309(+)
Misc Feature(9)310..312(+)
Misc Feature(10)319..321(+)
Misc Feature(11)337..339(+)
Misc Feature(12)337..339(+)
Misc Feature(13)370..480(+)
Misc Feature(14)370..423(+)
Misc Feature(15)391..393(+)
Misc Feature(16)421..423(+)
Misc Feature(17)505..615(+)
Misc Feature(18)667..669(+)
Misc Feature(19)688..1392(+)
Misc Feature(20)688..693(+)
Misc Feature(21)688..690(+)
Misc Feature(22)691..1401(+)
Misc Feature(23)691..693(+)
Misc Feature(24)811..1266(+)
Misc Feature(25)1198..1200(+)
Misc Feature(26)1246..1329(+)
Exon (1)1..118
Gene:F7
Gene Synonym:
Exon (2)119..184
Gene:F7
Gene Synonym:
Exon (3)185..345
Gene:F7
Gene Synonym:
Exon (4)346..370
Gene:F7
Gene Synonym:
Exon (5)371..484
Gene:F7
Gene Synonym:
Exon (6)485..625
Gene:F7
Gene Synonym:
Exon (7)626..735
Gene:F7
Gene Synonym:
Exon (8)736..859
Gene:F7
Gene Synonym:
Exon (9)860..3128
Gene:F7
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000131
Organism Homo sapiens (human)
Definition Homo sapiens coagulation factor VII (serum prothrombin conversion accelerator) (F7), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000131

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTCTCCC AGGCCCTCAG GCTCCTCTGC CTTCTGCTTG GGCTTCAGGG CTGCCTGGCT 
GCAGGCGGGG TCGCTAAGGC CTCAGGAGGA GAAACACGGG ACATGCCGTG GAAGCCGGGG
CCTCACAGAG TCTTCGTAAC CCAGGAGGAA GCCCACGGCG TCCTGCACCG GCGCCGGCGC
GCCAACGCGT TCCTGGAGGA GCTGCGGCCG GGCTCCCTGG AGAGGGAGTG CAAGGAGGAG
CAGTGCTCCT TCGAGGAGGC CCGGGAGATC TTCAAGGACG CGGAGAGGAC GAAGCTGTTC
TGGATTTCTT ACAGTGATGG GGACCAGTGT GCCTCAAGTC CATGCCAGAA TGGGGGCTCC
TGCAAGGACC AGCTCCAGTC CTATATCTGC TTCTGCCTCC CTGCCTTCGA GGGCCGGAAC
TGTGAGACGC ACAAGGATGA CCAGCTGATC TGTGTGAACG AGAACGGCGG CTGTGAGCAG
TACTGCAGTG ACCACACGGG CACCAAGCGC TCCTGTCGGT GCCACGAGGG GTACTCTCTG
CTGGCAGACG GGGTGTCCTG CACACCCACA GTTGAATATC CATGTGGAAA AATACCTATT
CTAGAAAAAA GAAATGCCAG CAAACCCCAA GGCCGAATTG TGGGGGGCAA GGTGTGCCCC
AAAGGGGAGT GTCCATGGCA GGTCCTGTTG TTGGTGAATG GAGCTCAGTT GTGTGGGGGG
ACCCTGATCA ACACCATCTG GGTGGTCTCC GCGGCCCACT GTTTCGACAA AATCAAGAAC
TGGAGGAACC TGATCGCGGT GCTGGGCGAG CACGACCTCA GCGAGCACGA CGGGGATGAG
CAGAGCCGGC GGGTGGCGCA GGTCATCATC CCCAGCACGT ACGTCCCGGG CACCACCAAC
CACGACATCG CGCTGCTCCG CCTGCACCAG CCCGTGGTCC TCACTGACCA TGTGGTGCCC
CTCTGCCTGC CCGAACGGAC GTTCTCTGAG AGGACGCTGG CCTTCGTGCG CTTCTCATTG
GTCAGCGGCT GGGGCCAGCT GCTGGACCGT GGCGCCACGG CCCTGGAGCT CATGGTCCTC
AACGTGCCCC GGCTGATGAC CCAGGACTGC CTGCAGCAGT CACGGAAGGT GGGAGACTCC
CCAAATATCA CGGAGTACAT GTTCTGTGCC GGCTACTCGG ATGGCAGCAA GGACTCCTGC
AAGGGGGACA GTGGAGGCCC ACATGCCACC CACTACCGGG GCACGTGGTA CCTGACGGGC
ATCGTCAGCT GGGGCCAGGG CTGCGCAACC GTGGGCCACT TTGGGGTGTA CACCAGGGTC
TCCCAGTACA TCGAGTGGCT GCAAAAGCTC ATGCGCTCAG AGCCACGCCC AGGAGTCCTC
CTGCGAGCCC CATTTCCCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25271
Clone ID Related Accession (Same CDS sequence) NM_019616
Accession Version NM_019616.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1335bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 16-AUG-2015
Organism Homo sapiens (human)
Product coagulation factor VII isoform b precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK310779.1, BC130468.1, AL137002.19 and AI076552.1. On May 31, 2012 this sequence version replaced gi:116805323. Transcript Variant: This variant (2) lacks an exon in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (b) is shorter than isoform a. This isoform (b) may undergo proteolytic processing similar to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. A downstream translational start codon is selected for this RefSeq based on its better conservation in mammalian species. An upstream in-frame start codon is also present and would result in a protein that is 15 aa longer at the N-terminus, but this start codon is poorly conserved and located within a protein-binding site identified in the promoter region, as described in PubMed: 8576177. Leaky scanning by ribosomes may allow translation initiation at the downstream start codon. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:8576177) ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BC130468.1, AL531727.3 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2155590 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGTCTCCC AGGCCCTCAG GCTCCTCTGC CTTCTGCTTG GGCTTCAGGG CTGCCTGGCT 
GCAGTCTTCG TAACCCAGGA GGAAGCCCAC GGCGTCCTGC ACCGGCGCCG GCGCGCCAAC
GCGTTCCTGG AGGAGCTGCG GCCGGGCTCC CTGGAGAGGG AGTGCAAGGA GGAGCAGTGC
TCCTTCGAGG AGGCCCGGGA GATCTTCAAG GACGCGGAGA GGACGAAGCT GTTCTGGATT
TCTTACAGTG ATGGGGACCA GTGTGCCTCA AGTCCATGCC AGAATGGGGG CTCCTGCAAG
GACCAGCTCC AGTCCTATAT CTGCTTCTGC CTCCCTGCCT TCGAGGGCCG GAACTGTGAG
ACGCACAAGG ATGACCAGCT GATCTGTGTG AACGAGAACG GCGGCTGTGA GCAGTACTGC
AGTGACCACA CGGGCACCAA GCGCTCCTGT CGGTGCCACG AGGGGTACTC TCTGCTGGCA
GACGGGGTGT CCTGCACACC CACAGTTGAA TATCCATGTG GAAAAATACC TATTCTAGAA
AAAAGAAATG CCAGCAAACC CCAAGGCCGA ATTGTGGGGG GCAAGGTGTG CCCCAAAGGG
GAGTGTCCAT GGCAGGTCCT GTTGTTGGTG AATGGAGCTC AGTTGTGTGG GGGGACCCTG
ATCAACACCA TCTGGGTGGT CTCCGCGGCC CACTGTTTCG ACAAAATCAA GAACTGGAGG
AACCTGATCG CGGTGCTGGG CGAGCACGAC CTCAGCGAGC ACGACGGGGA TGAGCAGAGC
CGGCGGGTGG CGCAGGTCAT CATCCCCAGC ACGTACGTCC CGGGCACCAC CAACCACGAC
ATCGCGCTGC TCCGCCTGCA CCAGCCCGTG GTCCTCACTG ACCATGTGGT GCCCCTCTGC
CTGCCCGAAC GGACGTTCTC TGAGAGGACG CTGGCCTTCG TGCGCTTCTC ATTGGTCAGC
GGCTGGGGCC AGCTGCTGGA CCGTGGCGCC ACGGCCCTGG AGCTCATGGT CCTCAACGTG
CCCCGGCTGA TGACCCAGGA CTGCCTGCAG CAGTCACGGA AGGTGGGAGA CTCCCCAAAT
ATCACGGAGT ACATGTTCTG TGCCGGCTAC TCGGATGGCA GCAAGGACTC CTGCAAGGGG
GACAGTGGAG GCCCACATGC CACCCACTAC CGGGGCACGT GGTACCTGAC GGGCATCGTC
AGCTGGGGCC AGGGCTGCGC AACCGTGGGC CACTTTGGGG TGTACACCAG GGTCTCCCAG
TACATCGAGT GGCTGCAAAA GCTCATGCGC TCAGAGCCAC GCCCAGGAGT CCTCCTGCGA
GCCCCATTTC CCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_062562.1
CDS55..1389
Misc Feature(1)115..300(+)
Misc Feature(2)271..273(+)
Misc Feature(3)304..414(+)
Misc Feature(4)304..357(+)
Misc Feature(5)439..549(+)
Misc Feature(6)601..603(+)
Misc Feature(7)622..1326(+)
Misc Feature(8)622..624(+)
Misc Feature(9)625..1335(+)
Misc Feature(10)625..627(+)
Misc Feature(11)745..1200(+)
Misc Feature(12)1132..1134(+)
Misc Feature(13)1180..1263(+)
Exon (1)1..118
Gene:F7
Gene Synonym:
Exon (2)119..279
Gene:F7
Gene Synonym:
Exon (3)280..304
Gene:F7
Gene Synonym:
Exon (4)305..418
Gene:F7
Gene Synonym:
Exon (5)419..559
Gene:F7
Gene Synonym:
Exon (6)560..669
Gene:F7
Gene Synonym:
Exon (7)670..793
Gene:F7
Gene Synonym:
Exon (8)794..3062
Gene:F7
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_019616
Organism Homo sapiens (human)
Definition Homo sapiens coagulation factor VII (serum prothrombin conversion accelerator) (F7), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_019616

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGTCTCCC AGGCCCTCAG GCTCCTCTGC CTTCTGCTTG GGCTTCAGGG CTGCCTGGCT 
GCAGTCTTCG TAACCCAGGA GGAAGCCCAC GGCGTCCTGC ACCGGCGCCG GCGCGCCAAC
GCGTTCCTGG AGGAGCTGCG GCCGGGCTCC CTGGAGAGGG AGTGCAAGGA GGAGCAGTGC
TCCTTCGAGG AGGCCCGGGA GATCTTCAAG GACGCGGAGA GGACGAAGCT GTTCTGGATT
TCTTACAGTG ATGGGGACCA GTGTGCCTCA AGTCCATGCC AGAATGGGGG CTCCTGCAAG
GACCAGCTCC AGTCCTATAT CTGCTTCTGC CTCCCTGCCT TCGAGGGCCG GAACTGTGAG
ACGCACAAGG ATGACCAGCT GATCTGTGTG AACGAGAACG GCGGCTGTGA GCAGTACTGC
AGTGACCACA CGGGCACCAA GCGCTCCTGT CGGTGCCACG AGGGGTACTC TCTGCTGGCA
GACGGGGTGT CCTGCACACC CACAGTTGAA TATCCATGTG GAAAAATACC TATTCTAGAA
AAAAGAAATG CCAGCAAACC CCAAGGCCGA ATTGTGGGGG GCAAGGTGTG CCCCAAAGGG
GAGTGTCCAT GGCAGGTCCT GTTGTTGGTG AATGGAGCTC AGTTGTGTGG GGGGACCCTG
ATCAACACCA TCTGGGTGGT CTCCGCGGCC CACTGTTTCG ACAAAATCAA GAACTGGAGG
AACCTGATCG CGGTGCTGGG CGAGCACGAC CTCAGCGAGC ACGACGGGGA TGAGCAGAGC
CGGCGGGTGG CGCAGGTCAT CATCCCCAGC ACGTACGTCC CGGGCACCAC CAACCACGAC
ATCGCGCTGC TCCGCCTGCA CCAGCCCGTG GTCCTCACTG ACCATGTGGT GCCCCTCTGC
CTGCCCGAAC GGACGTTCTC TGAGAGGACG CTGGCCTTCG TGCGCTTCTC ATTGGTCAGC
GGCTGGGGCC AGCTGCTGGA CCGTGGCGCC ACGGCCCTGG AGCTCATGGT CCTCAACGTG
CCCCGGCTGA TGACCCAGGA CTGCCTGCAG CAGTCACGGA AGGTGGGAGA CTCCCCAAAT
ATCACGGAGT ACATGTTCTG TGCCGGCTAC TCGGATGGCA GCAAGGACTC CTGCAAGGGG
GACAGTGGAG GCCCACATGC CACCCACTAC CGGGGCACGT GGTACCTGAC GGGCATCGTC
AGCTGGGGCC AGGGCTGCGC AACCGTGGGC CACTTTGGGG TGTACACCAG GGTCTCCCAG
TACATCGAGT GGCTGCAAAA GCTCATGCGC TCAGAGCCAC GCCCAGGAGT CCTCCTGCGA
GCCCCATTTC CCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25174
Clone ID Related Accession (Same CDS sequence) NM_001267554
Accession Version NM_001267554.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1149bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 16-AUG-2015
Organism Homo sapiens (human)
Product coagulation factor VII isoform c precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK310779.1, AK298404.1, AL137002.19 and AI076552.1. Transcript Variant: This variant (3) lacks three consecutive exons in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (c) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. A downstream translational start codon is selected for this RefSeq based on its better conservation in mammalian species. An upstream in-frame start codon is also present and would result in a protein that is 15 aa longer at the N-terminus, but this start codon is poorly conserved and located within a protein-binding site identified in the promoter region, as described in PubMed: 8576177. Leaky scanning by ribosomes may allow translation initiation at the downstream start codon. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:8576177) ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AK298404.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2155590 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGTCTCCC AGGCCCTCAG GCTCCTCTGC CTTCTGCTTG GGCTTCAGGG CTGCCTGGCT 
GCAGATGGGG ACCAGTGTGC CTCAAGTCCA TGCCAGAATG GGGGCTCCTG CAAGGACCAG
CTCCAGTCCT ATATCTGCTT CTGCCTCCCT GCCTTCGAGG GCCGGAACTG TGAGACGCAC
AAGGATGACC AGCTGATCTG TGTGAACGAG AACGGCGGCT GTGAGCAGTA CTGCAGTGAC
CACACGGGCA CCAAGCGCTC CTGTCGGTGC CACGAGGGGT ACTCTCTGCT GGCAGACGGG
GTGTCCTGCA CACCCACAGT TGAATATCCA TGTGGAAAAA TACCTATTCT AGAAAAAAGA
AATGCCAGCA AACCCCAAGG CCGAATTGTG GGGGGCAAGG TGTGCCCCAA AGGGGAGTGT
CCATGGCAGG TCCTGTTGTT GGTGAATGGA GCTCAGTTGT GTGGGGGGAC CCTGATCAAC
ACCATCTGGG TGGTCTCCGC GGCCCACTGT TTCGACAAAA TCAAGAACTG GAGGAACCTG
ATCGCGGTGC TGGGCGAGCA CGACCTCAGC GAGCACGACG GGGATGAGCA GAGCCGGCGG
GTGGCGCAGG TCATCATCCC CAGCACGTAC GTCCCGGGCA CCACCAACCA CGACATCGCG
CTGCTCCGCC TGCACCAGCC CGTGGTCCTC ACTGACCATG TGGTGCCCCT CTGCCTGCCC
GAACGGACGT TCTCTGAGAG GACGCTGGCC TTCGTGCGCT TCTCATTGGT CAGCGGCTGG
GGCCAGCTGC TGGACCGTGG CGCCACGGCC CTGGAGCTCA TGGTCCTCAA CGTGCCCCGG
CTGATGACCC AGGACTGCCT GCAGCAGTCA CGGAAGGTGG GAGACTCCCC AAATATCACG
GAGTACATGT TCTGTGCCGG CTACTCGGAT GGCAGCAAGG ACTCCTGCAA GGGGGACAGT
GGAGGCCCAC ATGCCACCCA CTACCGGGGC ACGTGGTACC TGACGGGCAT CGTCAGCTGG
GGCCAGGGCT GCGCAACCGT GGGCCACTTT GGGGTGTACA CCAGGGTCTC CCAGTACATC
GAGTGGCTGC AAAAGCTCAT GCGCTCAGAG CCACGCCCAG GAGTCCTCCT GCGAGCCCCA
TTTCCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001254483.1
CDS55..1203
Misc Feature(1)118..228(+)
Misc Feature(2)118..171(+)
Misc Feature(3)253..363(+)
Misc Feature(4)436..1140(+)
Misc Feature(5)439..1149(+)
Misc Feature(6)439..441(+)
Misc Feature(7)559..1014(+)
Misc Feature(8)994..1077(+)
Exon (1)1..118
Gene:F7
Gene Synonym:
Exon (2)119..232
Gene:F7
Gene Synonym:
Exon (3)233..373
Gene:F7
Gene Synonym:
Exon (4)374..483
Gene:F7
Gene Synonym:
Exon (5)484..607
Gene:F7
Gene Synonym:
Exon (6)608..2876
Gene:F7
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001267554
Organism Homo sapiens (human)
Definition Homo sapiens coagulation factor VII (serum prothrombin conversion accelerator) (F7), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001267554

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGTCTCCC AGGCCCTCAG GCTCCTCTGC CTTCTGCTTG GGCTTCAGGG CTGCCTGGCT 
GCAGATGGGG ACCAGTGTGC CTCAAGTCCA TGCCAGAATG GGGGCTCCTG CAAGGACCAG
CTCCAGTCCT ATATCTGCTT CTGCCTCCCT GCCTTCGAGG GCCGGAACTG TGAGACGCAC
AAGGATGACC AGCTGATCTG TGTGAACGAG AACGGCGGCT GTGAGCAGTA CTGCAGTGAC
CACACGGGCA CCAAGCGCTC CTGTCGGTGC CACGAGGGGT ACTCTCTGCT GGCAGACGGG
GTGTCCTGCA CACCCACAGT TGAATATCCA TGTGGAAAAA TACCTATTCT AGAAAAAAGA
AATGCCAGCA AACCCCAAGG CCGAATTGTG GGGGGCAAGG TGTGCCCCAA AGGGGAGTGT
CCATGGCAGG TCCTGTTGTT GGTGAATGGA GCTCAGTTGT GTGGGGGGAC CCTGATCAAC
ACCATCTGGG TGGTCTCCGC GGCCCACTGT TTCGACAAAA TCAAGAACTG GAGGAACCTG
ATCGCGGTGC TGGGCGAGCA CGACCTCAGC GAGCACGACG GGGATGAGCA GAGCCGGCGG
GTGGCGCAGG TCATCATCCC CAGCACGTAC GTCCCGGGCA CCACCAACCA CGACATCGCG
CTGCTCCGCC TGCACCAGCC CGTGGTCCTC ACTGACCATG TGGTGCCCCT CTGCCTGCCC
GAACGGACGT TCTCTGAGAG GACGCTGGCC TTCGTGCGCT TCTCATTGGT CAGCGGCTGG
GGCCAGCTGC TGGACCGTGG CGCCACGGCC CTGGAGCTCA TGGTCCTCAA CGTGCCCCGG
CTGATGACCC AGGACTGCCT GCAGCAGTCA CGGAAGGTGG GAGACTCCCC AAATATCACG
GAGTACATGT TCTGTGCCGG CTACTCGGAT GGCAGCAAGG ACTCCTGCAA GGGGGACAGT
GGAGGCCCAC ATGCCACCCA CTACCGGGGC ACGTGGTACC TGACGGGCAT CGTCAGCTGG
GGCCAGGGCT GCGCAACCGT GGGCCACTTT GGGGTGTACA CCAGGGTCTC CCAGTACATC
GAGTGGCTGC AAAAGCTCAT GCGCTCAGAG CCACGCCCAG GAGTCCTCCT GCGAGCCCCA
TTTCCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57662
Clone ID Related Accession (Same CDS sequence) XM_011537474
Accession Version XM_011537474.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1443bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product coagulation factor VII isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_027140.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGTCTCCC AGGCCCTCAG GCTCCTCTGC CTTCTGCTTG GGCTTCAGGG CTGCCTGGCT 
GCAGTCTTCG TAACCCAGGA GGAAGCCCAC GGCGTCCTGC ACCGGCGCCG GCGCGCCAAC
GCGTTCCTGG AGGAGCTGCG GCCGGGCTCC CTGGAGAGGG AGTGCAAGGA GGAGCAGTGC
TCCTTCGAGG AGGCCCGGGA GATCTTCAAG GACGCGGAGA GGACGAAGCT GTTCTGGATT
TCTTACAGTG ATGGGGACCA GTGTGCCTCA AGTCCATGCC AGAATGGGGG CTCCTGCAAG
GACCAGCTCC AGTCCTATAT CTGCTTCTGC CTCCCTGCCT TCGAGGGCCG GAACTGTGAG
ACGCAAGAGA GCCCAGCAAG CTGGAGGAGG CTGAAGAGAG AGGCTTCCTG CTGGTCATCT
GGGTCCAGAA TGCCTGGAGA TCTCTGCTCA GCCCTGGTGC CCAGCAGCCC TGACAAGGAT
GACCAGCTGA TCTGTGTGAA CGAGAACGGC GGCTGTGAGC AGTACTGCAG TGACCACACG
GGCACCAAGC GCTCCTGTCG GTGCCACGAG GGGTACTCTC TGCTGGCAGA CGGGGTGTCC
TGCACACCCA CAGTTGAATA TCCATGTGGA AAAATACCTA TTCTAGAAAA AAGAAATGCC
AGCAAACCCC AAGGCCGAAT TGTGGGGGGC AAGGTGTGCC CCAAAGGGGA GTGTCCATGG
CAGGTCCTGT TGTTGGTGAA TGGAGCTCAG TTGTGTGGGG GGACCCTGAT CAACACCATC
TGGGTGGTCT CCGCGGCCCA CTGTTTCGAC AAAATCAAGA ACTGGAGGAA CCTGATCGCG
GTGCTGGGCG AGCACGACCT CAGCGAGCAC GACGGGGATG AGCAGAGCCG GCGGGTGGCG
CAGGTCATCA TCCCCAGCAC GTACGTCCCG GGCACCACCA ACCACGACAT CGCGCTGCTC
CGCCTGCACC AGCCCGTGGT CCTCACTGAC CATGTGGTGC CCCTCTGCCT GCCCGAACGG
ACGTTCTCTG AGAGGACGCT GGCCTTCGTG CGCTTCTCAT TGGTCAGCGG CTGGGGCCAG
CTGCTGGACC GTGGCGCCAC GGCCCTGGAG CTCATGGTCC TCAACGTGCC CCGGCTGATG
ACCCAGGACT GCCTGCAGCA GTCACGGAAG GTGGGAGACT CCCCAAATAT CACGGAGTAC
ATGTTCTGTG CCGGCTACTC GGATGGCAGC AAGGACTCCT GCAAGGGGGA CAGTGGAGGC
CCACATGCCA CCCACTACCG GGGCACGTGG TACCTGACGG GCATCGTCAG CTGGGGCCAG
GGCTGCGCAA CCGTGGGCCA CTTTGGGGTG TACACCAGGG TCTCCCAGTA CATCGAGTGG
CTGCAAAAGC TCATGCGCTC AGAGCCACGC CCAGGAGTCC TCCTGCGAGC CCCATTTCCC
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011535776.1
CDS28..1470
Misc Feature(1)88..273(+)
Misc Feature(2)277..387(+)
Misc Feature(3)277..330(+)
Misc Feature(4)520..630(+)
Misc Feature(5)703..1407(+)
Misc Feature(6)706..1416(+)
Misc Feature(7)706..708(+)
Misc Feature(8)826..1281(+)
Misc Feature(9)1261..1344(+)
Translation

Target ORF information:

RefSeq Version XM_011537474
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens coagulation factor VII (serum prothrombin conversion accelerator) (F7), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011537474

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGTCTCCC AGGCCCTCAG GCTCCTCTGC CTTCTGCTTG GGCTTCAGGG CTGCCTGGCT 
GCAGTCTTCG TAACCCAGGA GGAAGCCCAC GGCGTCCTGC ACCGGCGCCG GCGCGCCAAC
GCGTTCCTGG AGGAGCTGCG GCCGGGCTCC CTGGAGAGGG AGTGCAAGGA GGAGCAGTGC
TCCTTCGAGG AGGCCCGGGA GATCTTCAAG GACGCGGAGA GGACGAAGCT GTTCTGGATT
TCTTACAGTG ATGGGGACCA GTGTGCCTCA AGTCCATGCC AGAATGGGGG CTCCTGCAAG
GACCAGCTCC AGTCCTATAT CTGCTTCTGC CTCCCTGCCT TCGAGGGCCG GAACTGTGAG
ACGCAAGAGA GCCCAGCAAG CTGGAGGAGG CTGAAGAGAG AGGCTTCCTG CTGGTCATCT
GGGTCCAGAA TGCCTGGAGA TCTCTGCTCA GCCCTGGTGC CCAGCAGCCC TGACAAGGAT
GACCAGCTGA TCTGTGTGAA CGAGAACGGC GGCTGTGAGC AGTACTGCAG TGACCACACG
GGCACCAAGC GCTCCTGTCG GTGCCACGAG GGGTACTCTC TGCTGGCAGA CGGGGTGTCC
TGCACACCCA CAGTTGAATA TCCATGTGGA AAAATACCTA TTCTAGAAAA AAGAAATGCC
AGCAAACCCC AAGGCCGAAT TGTGGGGGGC AAGGTGTGCC CCAAAGGGGA GTGTCCATGG
CAGGTCCTGT TGTTGGTGAA TGGAGCTCAG TTGTGTGGGG GGACCCTGAT CAACACCATC
TGGGTGGTCT CCGCGGCCCA CTGTTTCGAC AAAATCAAGA ACTGGAGGAA CCTGATCGCG
GTGCTGGGCG AGCACGACCT CAGCGAGCAC GACGGGGATG AGCAGAGCCG GCGGGTGGCG
CAGGTCATCA TCCCCAGCAC GTACGTCCCG GGCACCACCA ACCACGACAT CGCGCTGCTC
CGCCTGCACC AGCCCGTGGT CCTCACTGAC CATGTGGTGC CCCTCTGCCT GCCCGAACGG
ACGTTCTCTG AGAGGACGCT GGCCTTCGTG CGCTTCTCAT TGGTCAGCGG CTGGGGCCAG
CTGCTGGACC GTGGCGCCAC GGCCCTGGAG CTCATGGTCC TCAACGTGCC CCGGCTGATG
ACCCAGGACT GCCTGCAGCA GTCACGGAAG GTGGGAGACT CCCCAAATAT CACGGAGTAC
ATGTTCTGTG CCGGCTACTC GGATGGCAGC AAGGACTCCT GCAAGGGGGA CAGTGGAGGC
CCACATGCCA CCCACTACCG GGGCACGTGG TACCTGACGG GCATCGTCAG CTGGGGCCAG
GGCTGCGCAA CCGTGGGCCA CTTTGGGGTG TACACCAGGG TCTCCCAGTA CATCGAGTGG
CTGCAAAAGC TCATGCGCTC AGAGCCACGC CCAGGAGTCC TCCTGCGAGC CCCATTTCCC
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57663
Clone ID Related Accession (Same CDS sequence) XM_011537475
Accession Version XM_011537475.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1257bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product coagulation factor VII isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_027140.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGTCTCCC AGGCCCTCAG GCTCCTCTGC CTTCTGCTTG GGCTTCAGGG CTGCCTGGCT 
GCAGATGGGG ACCAGTGTGC CTCAAGTCCA TGCCAGAATG GGGGCTCCTG CAAGGACCAG
CTCCAGTCCT ATATCTGCTT CTGCCTCCCT GCCTTCGAGG GCCGGAACTG TGAGACGCAA
GAGAGCCCAG CAAGCTGGAG GAGGCTGAAG AGAGAGGCTT CCTGCTGGTC ATCTGGGTCC
AGAATGCCTG GAGATCTCTG CTCAGCCCTG GTGCCCAGCA GCCCTGACAA GGATGACCAG
CTGATCTGTG TGAACGAGAA CGGCGGCTGT GAGCAGTACT GCAGTGACCA CACGGGCACC
AAGCGCTCCT GTCGGTGCCA CGAGGGGTAC TCTCTGCTGG CAGACGGGGT GTCCTGCACA
CCCACAGTTG AATATCCATG TGGAAAAATA CCTATTCTAG AAAAAAGAAA TGCCAGCAAA
CCCCAAGGCC GAATTGTGGG GGGCAAGGTG TGCCCCAAAG GGGAGTGTCC ATGGCAGGTC
CTGTTGTTGG TGAATGGAGC TCAGTTGTGT GGGGGGACCC TGATCAACAC CATCTGGGTG
GTCTCCGCGG CCCACTGTTT CGACAAAATC AAGAACTGGA GGAACCTGAT CGCGGTGCTG
GGCGAGCACG ACCTCAGCGA GCACGACGGG GATGAGCAGA GCCGGCGGGT GGCGCAGGTC
ATCATCCCCA GCACGTACGT CCCGGGCACC ACCAACCACG ACATCGCGCT GCTCCGCCTG
CACCAGCCCG TGGTCCTCAC TGACCATGTG GTGCCCCTCT GCCTGCCCGA ACGGACGTTC
TCTGAGAGGA CGCTGGCCTT CGTGCGCTTC TCATTGGTCA GCGGCTGGGG CCAGCTGCTG
GACCGTGGCG CCACGGCCCT GGAGCTCATG GTCCTCAACG TGCCCCGGCT GATGACCCAG
GACTGCCTGC AGCAGTCACG GAAGGTGGGA GACTCCCCAA ATATCACGGA GTACATGTTC
TGTGCCGGCT ACTCGGATGG CAGCAAGGAC TCCTGCAAGG GGGACAGTGG AGGCCCACAT
GCCACCCACT ACCGGGGCAC GTGGTACCTG ACGGGCATCG TCAGCTGGGG CCAGGGCTGC
GCAACCGTGG GCCACTTTGG GGTGTACACC AGGGTCTCCC AGTACATCGA GTGGCTGCAA
AAGCTCATGC GCTCAGAGCC ACGCCCAGGA GTCCTCCTGC GAGCCCCATT TCCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011535777.1
CDS21..1277
Misc Feature(1)84..194(+)
Misc Feature(2)84..137(+)
Misc Feature(3)327..437(+)
Misc Feature(4)510..1214(+)
Misc Feature(5)513..1223(+)
Misc Feature(6)513..515(+)
Misc Feature(7)633..1088(+)
Misc Feature(8)1068..1151(+)
Translation

Target ORF information:

RefSeq Version XM_011537475
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens coagulation factor VII (serum prothrombin conversion accelerator) (F7), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011537475

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGTCTCCC AGGCCCTCAG GCTCCTCTGC CTTCTGCTTG GGCTTCAGGG CTGCCTGGCT 
GCAGATGGGG ACCAGTGTGC CTCAAGTCCA TGCCAGAATG GGGGCTCCTG CAAGGACCAG
CTCCAGTCCT ATATCTGCTT CTGCCTCCCT GCCTTCGAGG GCCGGAACTG TGAGACGCAA
GAGAGCCCAG CAAGCTGGAG GAGGCTGAAG AGAGAGGCTT CCTGCTGGTC ATCTGGGTCC
AGAATGCCTG GAGATCTCTG CTCAGCCCTG GTGCCCAGCA GCCCTGACAA GGATGACCAG
CTGATCTGTG TGAACGAGAA CGGCGGCTGT GAGCAGTACT GCAGTGACCA CACGGGCACC
AAGCGCTCCT GTCGGTGCCA CGAGGGGTAC TCTCTGCTGG CAGACGGGGT GTCCTGCACA
CCCACAGTTG AATATCCATG TGGAAAAATA CCTATTCTAG AAAAAAGAAA TGCCAGCAAA
CCCCAAGGCC GAATTGTGGG GGGCAAGGTG TGCCCCAAAG GGGAGTGTCC ATGGCAGGTC
CTGTTGTTGG TGAATGGAGC TCAGTTGTGT GGGGGGACCC TGATCAACAC CATCTGGGTG
GTCTCCGCGG CCCACTGTTT CGACAAAATC AAGAACTGGA GGAACCTGAT CGCGGTGCTG
GGCGAGCACG ACCTCAGCGA GCACGACGGG GATGAGCAGA GCCGGCGGGT GGCGCAGGTC
ATCATCCCCA GCACGTACGT CCCGGGCACC ACCAACCACG ACATCGCGCT GCTCCGCCTG
CACCAGCCCG TGGTCCTCAC TGACCATGTG GTGCCCCTCT GCCTGCCCGA ACGGACGTTC
TCTGAGAGGA CGCTGGCCTT CGTGCGCTTC TCATTGGTCA GCGGCTGGGG CCAGCTGCTG
GACCGTGGCG CCACGGCCCT GGAGCTCATG GTCCTCAACG TGCCCCGGCT GATGACCCAG
GACTGCCTGC AGCAGTCACG GAAGGTGGGA GACTCCCCAA ATATCACGGA GTACATGTTC
TGTGCCGGCT ACTCGGATGG CAGCAAGGAC TCCTGCAAGG GGGACAGTGG AGGCCCACAT
GCCACCCACT ACCGGGGCAC GTGGTACCTG ACGGGCATCG TCAGCTGGGG CCAGGGCTGC
GCAACCGTGG GCCACTTTGG GGTGTACACC AGGGTCTCCC AGTACATCGA GTGGCTGCAA
AAGCTCATGC GCTCAGAGCC ACGCCCAGGA GTCCTCCTGC GAGCCCCATT TCCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu47412
Clone ID Related Accession (Same CDS sequence) XM_006719963
Accession Version XM_006719963.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1194bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product coagulation factor VII isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_027140.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578825304. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGTCTCCC AGGCCCTCAG GCTCCTCTGC CTTCTGCTTG GGCTTCAGGG CTGCCTGGCT 
GCAGTCTTCG TAACCCAGGA GGAAGCCCAC GGCGTCCTGC ACCGGCGCCG GCGCGCCAAC
GCGTTCCTGG AGGAGCTGCG GCCGGGCTCC CTGGAGAGGG AGTGCAAGGA GGAGCAGTGC
TCCTTCGAGG AGGCCCGGGA GATCTTCAAG GACGCGGAGA GGACGAAGCT GTTCTGGATT
TCTTACAGTG ATGGGGACCA GTGTGCCTCA AGTCCATGCC AGAATGGGGG CTCCTGCAAG
GACCAGCTCC AGTCCTATAT CTGCTTCTGC CTCCCTGCCT TCGAGGGCCG GAACTGTGAG
ACGCTTGAAT ATCCATGTGG AAAAATACCT ATTCTAGAAA AAAGAAATGC CAGCAAACCC
CAAGGCCGAA TTGTGGGGGG CAAGGTGTGC CCCAAAGGGG AGTGTCCATG GCAGGTCCTG
TTGTTGGTGA ATGGAGCTCA GTTGTGTGGG GGGACCCTGA TCAACACCAT CTGGGTGGTC
TCCGCGGCCC ACTGTTTCGA CAAAATCAAG AACTGGAGGA ACCTGATCGC GGTGCTGGGC
GAGCACGACC TCAGCGAGCA CGACGGGGAT GAGCAGAGCC GGCGGGTGGC GCAGGTCATC
ATCCCCAGCA CGTACGTCCC GGGCACCACC AACCACGACA TCGCGCTGCT CCGCCTGCAC
CAGCCCGTGG TCCTCACTGA CCATGTGGTG CCCCTCTGCC TGCCCGAACG GACGTTCTCT
GAGAGGACGC TGGCCTTCGT GCGCTTCTCA TTGGTCAGCG GCTGGGGCCA GCTGCTGGAC
CGTGGCGCCA CGGCCCTGGA GCTCATGGTC CTCAACGTGC CCCGGCTGAT GACCCAGGAC
TGCCTGCAGC AGTCACGGAA GGTGGGAGAC TCCCCAAATA TCACGGAGTA CATGTTCTGT
GCCGGCTACT CGGATGGCAG CAAGGACTCC TGCAAGGGGG ACAGTGGAGG CCCACATGCC
ACCCACTACC GGGGCACGTG GTACCTGACG GGCATCGTCA GCTGGGGCCA GGGCTGCGCA
ACCGTGGGCC ACTTTGGGGT GTACACCAGG GTCTCCCAGT ACATCGAGTG GCTGCAAAAG
CTCATGCGCT CAGAGCCACG CCCAGGAGTC CTCCTGCGAG CCCCATTTCC CTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006720026.1
CDS28..1221
Misc Feature(1)88..273(+)
Misc Feature(2)277..387(+)
Misc Feature(3)277..330(+)
Misc Feature(4)454..1158(+)
Misc Feature(5)457..1167(+)
Misc Feature(6)457..459(+)
Misc Feature(7)577..1032(+)
Misc Feature(8)1012..1095(+)
Translation

Target ORF information:

RefSeq Version XM_006719963
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens coagulation factor VII (serum prothrombin conversion accelerator) (F7), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006719963

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGTCTCCC AGGCCCTCAG GCTCCTCTGC CTTCTGCTTG GGCTTCAGGG CTGCCTGGCT 
GCAGTCTTCG TAACCCAGGA GGAAGCCCAC GGCGTCCTGC ACCGGCGCCG GCGCGCCAAC
GCGTTCCTGG AGGAGCTGCG GCCGGGCTCC CTGGAGAGGG AGTGCAAGGA GGAGCAGTGC
TCCTTCGAGG AGGCCCGGGA GATCTTCAAG GACGCGGAGA GGACGAAGCT GTTCTGGATT
TCTTACAGTG ATGGGGACCA GTGTGCCTCA AGTCCATGCC AGAATGGGGG CTCCTGCAAG
GACCAGCTCC AGTCCTATAT CTGCTTCTGC CTCCCTGCCT TCGAGGGCCG GAACTGTGAG
ACGCTTGAAT ATCCATGTGG AAAAATACCT ATTCTAGAAA AAAGAAATGC CAGCAAACCC
CAAGGCCGAA TTGTGGGGGG CAAGGTGTGC CCCAAAGGGG AGTGTCCATG GCAGGTCCTG
TTGTTGGTGA ATGGAGCTCA GTTGTGTGGG GGGACCCTGA TCAACACCAT CTGGGTGGTC
TCCGCGGCCC ACTGTTTCGA CAAAATCAAG AACTGGAGGA ACCTGATCGC GGTGCTGGGC
GAGCACGACC TCAGCGAGCA CGACGGGGAT GAGCAGAGCC GGCGGGTGGC GCAGGTCATC
ATCCCCAGCA CGTACGTCCC GGGCACCACC AACCACGACA TCGCGCTGCT CCGCCTGCAC
CAGCCCGTGG TCCTCACTGA CCATGTGGTG CCCCTCTGCC TGCCCGAACG GACGTTCTCT
GAGAGGACGC TGGCCTTCGT GCGCTTCTCA TTGGTCAGCG GCTGGGGCCA GCTGCTGGAC
CGTGGCGCCA CGGCCCTGGA GCTCATGGTC CTCAACGTGC CCCGGCTGAT GACCCAGGAC
TGCCTGCAGC AGTCACGGAA GGTGGGAGAC TCCCCAAATA TCACGGAGTA CATGTTCTGT
GCCGGCTACT CGGATGGCAG CAAGGACTCC TGCAAGGGGG ACAGTGGAGG CCCACATGCC
ACCCACTACC GGGGCACGTG GTACCTGACG GGCATCGTCA GCTGGGGCCA GGGCTGCGCA
ACCGTGGGCC ACTTTGGGGT GTACACCAGG GTCTCCCAGT ACATCGAGTG GCTGCAAAAG
CTCATGCGCT CAGAGCCACG CCCAGGAGTC CTCCTGCGAG CCCCATTTCC CTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57664
Clone ID Related Accession (Same CDS sequence) XM_011537476
Accession Version XM_011537476.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1095bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product coagulation factor VII isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_027140.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCTCAAG GCCCAGAAGA GAGCCCAGCA AGCTGGAGGA GGCTGAAGAG AGAGGCTTCC 
TGCTGGTCAT CTGGGTCCAG AATGCCTGGA GATCTCTGCT CAGCCCTGGT GCCCAGCAGC
CCTGACAAGG ATGACCAGCT GATCTGTGTG AACGAGAACG GCGGCTGTGA GCAGTACTGC
AGTGACCACA CGGGCACCAA GCGCTCCTGT CGGTGCCACG AGGGGTACTC TCTGCTGGCA
GACGGGGTGT CCTGCACACC CACAGTTGAA TATCCATGTG GAAAAATACC TATTCTAGAA
AAAAGAAATG CCAGCAAACC CCAAGGCCGA ATTGTGGGGG GCAAGGTGTG CCCCAAAGGG
GAGTGTCCAT GGCAGGTCCT GTTGTTGGTG AATGGAGCTC AGTTGTGTGG GGGGACCCTG
ATCAACACCA TCTGGGTGGT CTCCGCGGCC CACTGTTTCG ACAAAATCAA GAACTGGAGG
AACCTGATCG CGGTGCTGGG CGAGCACGAC CTCAGCGAGC ACGACGGGGA TGAGCAGAGC
CGGCGGGTGG CGCAGGTCAT CATCCCCAGC ACGTACGTCC CGGGCACCAC CAACCACGAC
ATCGCGCTGC TCCGCCTGCA CCAGCCCGTG GTCCTCACTG ACCATGTGGT GCCCCTCTGC
CTGCCCGAAC GGACGTTCTC TGAGAGGACG CTGGCCTTCG TGCGCTTCTC ATTGGTCAGC
GGCTGGGGCC AGCTGCTGGA CCGTGGCGCC ACGGCCCTGG AGCTCATGGT CCTCAACGTG
CCCCGGCTGA TGACCCAGGA CTGCCTGCAG CAGTCACGGA AGGTGGGAGA CTCCCCAAAT
ATCACGGAGT ACATGTTCTG TGCCGGCTAC TCGGATGGCA GCAAGGACTC CTGCAAGGGG
GACAGTGGAG GCCCACATGC CACCCACTAC CGGGGCACGT GGTACCTGAC GGGCATCGTC
AGCTGGGGCC AGGGCTGCGC AACCGTGGGC CACTTTGGGG TGTACACCAG GGTCTCCCAG
TACATCGAGT GGCTGCAAAA GCTCATGCGC TCAGAGCCAC GCCCAGGAGT CCTCCTGCGA
GCCCCATTTC CCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011535778.1
CDS399..1493
Misc Feature(1)543..653(+)
Misc Feature(2)726..1430(+)
Misc Feature(3)729..1439(+)
Misc Feature(4)729..731(+)
Misc Feature(5)849..1304(+)
Misc Feature(6)1284..1367(+)
Translation

Target ORF information:

RefSeq Version XM_011537476
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens coagulation factor VII (serum prothrombin conversion accelerator) (F7), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011537476

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCTCAAG GCCCAGAAGA GAGCCCAGCA AGCTGGAGGA GGCTGAAGAG AGAGGCTTCC 
TGCTGGTCAT CTGGGTCCAG AATGCCTGGA GATCTCTGCT CAGCCCTGGT GCCCAGCAGC
CCTGACAAGG ATGACCAGCT GATCTGTGTG AACGAGAACG GCGGCTGTGA GCAGTACTGC
AGTGACCACA CGGGCACCAA GCGCTCCTGT CGGTGCCACG AGGGGTACTC TCTGCTGGCA
GACGGGGTGT CCTGCACACC CACAGTTGAA TATCCATGTG GAAAAATACC TATTCTAGAA
AAAAGAAATG CCAGCAAACC CCAAGGCCGA ATTGTGGGGG GCAAGGTGTG CCCCAAAGGG
GAGTGTCCAT GGCAGGTCCT GTTGTTGGTG AATGGAGCTC AGTTGTGTGG GGGGACCCTG
ATCAACACCA TCTGGGTGGT CTCCGCGGCC CACTGTTTCG ACAAAATCAA GAACTGGAGG
AACCTGATCG CGGTGCTGGG CGAGCACGAC CTCAGCGAGC ACGACGGGGA TGAGCAGAGC
CGGCGGGTGG CGCAGGTCAT CATCCCCAGC ACGTACGTCC CGGGCACCAC CAACCACGAC
ATCGCGCTGC TCCGCCTGCA CCAGCCCGTG GTCCTCACTG ACCATGTGGT GCCCCTCTGC
CTGCCCGAAC GGACGTTCTC TGAGAGGACG CTGGCCTTCG TGCGCTTCTC ATTGGTCAGC
GGCTGGGGCC AGCTGCTGGA CCGTGGCGCC ACGGCCCTGG AGCTCATGGT CCTCAACGTG
CCCCGGCTGA TGACCCAGGA CTGCCTGCAG CAGTCACGGA AGGTGGGAGA CTCCCCAAAT
ATCACGGAGT ACATGTTCTG TGCCGGCTAC TCGGATGGCA GCAAGGACTC CTGCAAGGGG
GACAGTGGAG GCCCACATGC CACCCACTAC CGGGGCACGT GGTACCTGAC GGGCATCGTC
AGCTGGGGCC AGGGCTGCGC AACCGTGGGC CACTTTGGGG TGTACACCAG GGTCTCCCAG
TACATCGAGT GGCTGCAAAA GCTCATGCGC TCAGAGCCAC GCCCAGGAGT CCTCCTGCGA
GCCCCATTTC CCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57665
Clone ID Related Accession (Same CDS sequence) XM_011537477
Accession Version XM_011537477.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1404bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product coagulation factor VII isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_027140.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTCTCCC AGGCCCTCAG GCTCCTCTGC CTTCTGCTTG GGCTTCAGGG CTGCCTGGCT 
GCAGGAGGAA GCCCACGGCG TCCTGCACCG GCGCCGGCGC GCCAACGCGT TCCTGGAGGA
GCTGCGGCCG GGCTCCCTGG AGAGGGAGTG CAAGGAGGAG CAGTGCTCCT TCGAGGAGGC
CCGGGAGATC TTCAAGGACG CGGAGAGGAC GATGGGGACC AGTGTGCCTC AAGTCCATGC
CAGAATGGGG GCTCCTGCAA GGACCAGCTC CAGTCCTATA TCTGCTTCTG CCTCCCTGCC
TTCGAGGGCC GGAACTGTGA GACGCAAGAG AGCCCAGCAA GCTGGAGGAG GCTGAAGAGA
GAGGCTTCCT GCTGGTCATC TGGGTCCAGA ATGCCTGGAG ATCTCTGCTC AGCCCTGGTG
CCCAGCAGCC CTGACAAGGA TGACCAGCTG ATCTGTGTGA ACGAGAACGG CGGCTGTGAG
CAGTACTGCA GTGACCACAC GGGCACCAAG CGCTCCTGTC GGTGCCACGA GGGGTACTCT
CTGCTGGCAG ACGGGGTGTC CTGCACACCC ACAGTTGAAT ATCCATGTGG AAAAATACCT
ATTCTAGAAA AAAGAAATGC CAGCAAACCC CAAGGCCGAA TTGTGGGGGG CAAGGTGTGC
CCCAAAGGGG AGTGTCCATG GCAGGTCCTG TTGTTGGTGA ATGGAGCTCA GTTGTGTGGG
GGGACCCTGA TCAACACCAT CTGGGTGGTC TCCGCGGCCC ACTGTTTCGA CAAAATCAAG
AACTGGAGGA ACCTGATCGC GGTGCTGGGC GAGCACGACC TCAGCGAGCA CGACGGGGAT
GAGCAGAGCC GGCGGGTGGC GCAGGTCATC ATCCCCAGCA CGTACGTCCC GGGCACCACC
AACCACGACA TCGCGCTGCT CCGCCTGCAC CAGCCCGTGG TCCTCACTGA CCATGTGGTG
CCCCTCTGCC TGCCCGAACG GACGTTCTCT GAGAGGACGC TGGCCTTCGT GCGCTTCTCA
TTGGTCAGCG GCTGGGGCCA GCTGCTGGAC CGTGGCGCCA CGGCCCTGGA GCTCATGGTC
CTCAACGTGC CCCGGCTGAT GACCCAGGAC TGCCTGCAGC AGTCACGGAA GGTGGGAGAC
TCCCCAAATA TCACGGAGTA CATGTTCTGT GCCGGCTACT CGGATGGCAG CAAGGACTCC
TGCAAGGGGG ACAGTGGAGG CCCACATGCC ACCCACTACC GGGGCACGTG GTACCTGACG
GGCATCGTCA GCTGGGGCCA GGGCTGCGCA ACCGTGGGCC ACTTTGGGGT GTACACCAGG
GTCTCCCAGT ACATCGAGTG GCTGCAAAAG CTCATGCGCT CAGAGCCACG CCCAGGAGTC
CTCCTGCGAG CCCCATTTCC CTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011535779.1
CDS38..1441
Misc Feature(1)248..358(+)
Misc Feature(2)248..301(+)
Misc Feature(3)491..601(+)
Misc Feature(4)674..1378(+)
Misc Feature(5)677..1387(+)
Misc Feature(6)677..679(+)
Misc Feature(7)797..1252(+)
Misc Feature(8)1232..1315(+)
Translation

Target ORF information:

RefSeq Version XM_011537477
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens coagulation factor VII (serum prothrombin conversion accelerator) (F7), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011537477

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGTCTCCC AGGCCCTCAG GCTCCTCTGC CTTCTGCTTG GGCTTCAGGG CTGCCTGGCT 
GCAGGAGGAA GCCCACGGCG TCCTGCACCG GCGCCGGCGC GCCAACGCGT TCCTGGAGGA
GCTGCGGCCG GGCTCCCTGG AGAGGGAGTG CAAGGAGGAG CAGTGCTCCT TCGAGGAGGC
CCGGGAGATC TTCAAGGACG CGGAGAGGAC GATGGGGACC AGTGTGCCTC AAGTCCATGC
CAGAATGGGG GCTCCTGCAA GGACCAGCTC CAGTCCTATA TCTGCTTCTG CCTCCCTGCC
TTCGAGGGCC GGAACTGTGA GACGCAAGAG AGCCCAGCAA GCTGGAGGAG GCTGAAGAGA
GAGGCTTCCT GCTGGTCATC TGGGTCCAGA ATGCCTGGAG ATCTCTGCTC AGCCCTGGTG
CCCAGCAGCC CTGACAAGGA TGACCAGCTG ATCTGTGTGA ACGAGAACGG CGGCTGTGAG
CAGTACTGCA GTGACCACAC GGGCACCAAG CGCTCCTGTC GGTGCCACGA GGGGTACTCT
CTGCTGGCAG ACGGGGTGTC CTGCACACCC ACAGTTGAAT ATCCATGTGG AAAAATACCT
ATTCTAGAAA AAAGAAATGC CAGCAAACCC CAAGGCCGAA TTGTGGGGGG CAAGGTGTGC
CCCAAAGGGG AGTGTCCATG GCAGGTCCTG TTGTTGGTGA ATGGAGCTCA GTTGTGTGGG
GGGACCCTGA TCAACACCAT CTGGGTGGTC TCCGCGGCCC ACTGTTTCGA CAAAATCAAG
AACTGGAGGA ACCTGATCGC GGTGCTGGGC GAGCACGACC TCAGCGAGCA CGACGGGGAT
GAGCAGAGCC GGCGGGTGGC GCAGGTCATC ATCCCCAGCA CGTACGTCCC GGGCACCACC
AACCACGACA TCGCGCTGCT CCGCCTGCAC CAGCCCGTGG TCCTCACTGA CCATGTGGTG
CCCCTCTGCC TGCCCGAACG GACGTTCTCT GAGAGGACGC TGGCCTTCGT GCGCTTCTCA
TTGGTCAGCG GCTGGGGCCA GCTGCTGGAC CGTGGCGCCA CGGCCCTGGA GCTCATGGTC
CTCAACGTGC CCCGGCTGAT GACCCAGGAC TGCCTGCAGC AGTCACGGAA GGTGGGAGAC
TCCCCAAATA TCACGGAGTA CATGTTCTGT GCCGGCTACT CGGATGGCAG CAAGGACTCC
TGCAAGGGGG ACAGTGGAGG CCCACATGCC ACCCACTACC GGGGCACGTG GTACCTGACG
GGCATCGTCA GCTGGGGCCA GGGCTGCGCA ACCGTGGGCC ACTTTGGGGT GTACACCAGG
GTCTCCCAGT ACATCGAGTG GCTGCAAAAG CTCATGCGCT CAGAGCCACG CCCAGGAGTC
CTCCTGCGAG CCCCATTTCC CTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Coagulation factor VII is regulated by androgen receptor in breast cancer
Exp. Cell Res. 331 (1), 239-250 (2015)
Naderi A.


book

Sites involved in intra- and interdomain allostery associated with the activation of factor VIIa pinpointed by hydrogen-deuterium exchange and electron transfer dissociation mass spectrometry
J. Biol. Chem. 289 (51), 35388-35396 (2014)
Song H, Olsen OH, Persson E and Rand KD.


book

Using an in situ proximity ligation assay to systematically profile endogenous protein-protein interactions in a pathway network
J. Proteome Res. 13 (12), 5339-5346 (2014)
Chen TC, Lin KT, Chen CH, Lee SA, Lee PY, Liu YW, Kuo YL, Wang FS, Lai JM and Huang CY.


book

The Eph tyrosine kinase receptors EphB2 and EphA2 are novel proteolytic substrates of tissue factor/coagulation factor VIIa
J. Biol. Chem. 289 (47), 32379-32391 (2014)
Eriksson O, Ramstrom M, Hornaeus K, Bergquist J, Mokhtari D and Siegbahn A.


book

Functional characterization of the human factor VII 5'-flanking region
J. Biol. Chem. 271 (3), 1738-1747 (1996)
Pollak ES, Hung HL, Godin W, Overton GC and High KA.


book

Detection of two missense mutations and characterization of a repeat polymorphism in the factor VII gene (F7)
Hum. Genet. 89 (5), 497-502 (1992)
Marchetti G, Patracchini P, Gemmati D, DeRosa V, Pinotti M, Rodorigo G, Casonato A, Girolami A and Bernardi F.


book

Purification and characterization of factor VII 304-Gln: a variant molecule with reduced activity isolated from a clinically unaffected male
Blood 78 (1), 132-140 (1991)
O'Brien DP, Gale KM, Anderson JS, McVey JH, Miller GJ, Meade TW and Tuddenham EG.


book

Human plasma and recombinant factor VII. Characterization of O-glycosylations at serine residues 52 and 60 and effects of site-directed mutagenesis of serine 52 to alanine
J. Biol. Chem. 266 (17), 11051-11057 (1991)
Bjoern S, Foster DC, Thim L, Wiberg FC, Christensen M, Komiyama Y, Pedersen AH and Kisiel W.


book

A new trisaccharide sugar chain linked to a serine residue in the first EGF-like domain of clotting factors VII and IX and protein Z
Adv. Exp. Med. Biol. 281, 121-131 (1990)
Iwanaga S, Nishimura H, Kawabata S, Kisiel W, Hase S and Ikenaka T.


book

Amino acid sequence and posttranslational modifications of human factor VIIa from plasma and transfected baby hamster kidney cells
Biochemistry 27 (20), 7785-7793 (1988)
Thim L, Bjoern S, Christensen M, Nicolaisen EM, Lund-Hansen T, Pedersen AH and Hedner U.


 
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