GOLGA2 cDNA ORF clone, Homo sapiens(human)
| Gene Symbol | GOLGA2 |
| Entrez Gene ID | 2801 |
| Full Name | golgin A2 |
| Synonyms | GM130 |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Homo sapiens(human) |
| Genome | |
| Summary | The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Feb 2010]. |
| Disorder MIM: |
mRNA and Protein(s)
| mRNA | Protein | Name |
|---|---|---|
| NM_004486.4 | NP_004477.3 | golgin subfamily A member 2 |
| NM_001366246.1 | NP_001353175.1 | golgin subfamily A member 2 isoform 3 |
| NM_004486.5 | NP_004477.4 | golgin subfamily A member 2 isoform 2 |
| XM_005251932.1 | XP_005251989.1 | golgin subfamily A member 2 isoform X3 |
| XM_005251931.1 | XP_005251988.1 | golgin subfamily A member 2 isoform X2 |
| NM_001366244.1 | NP_001353173.1 | golgin subfamily A member 2 isoform 1 |
| XM_005251930.1 | XP_005251987.1 | golgin subfamily A member 2 isoform X1 |
| Mus musculus (house mouse) | Golga2 | NP_598613.2 |
| Danio rerio (zebrafish) | golga2 | NP_001120806.1 |
| Homo sapiens (human) | GOLGA2 | NP_004477.3 |
| Pan troglodytes (chimpanzee) | GOLGA2 | XP_001156160.2 |
| GOLGA2 | XP_001112219.2 | |
| Canis lupus familiaris (dog) | GOLGA2 | XP_537828.3 |
| Xenopus tropicalis (tropical clawed frog) | LOC100492442 | XP_002935553.2 |
| Bos taurus (cattle) | GOLGA2 | NP_001179124.1 |
| Anopheles gambiae (African malaria mosquito) | AgaP_AGAP011337 | XP_309313.4 |
| Rattus norvegicus (Norway rat) | Golga2 | NP_072118.2 |
| Gallus gallus (chicken) | GOLGA2 | XP_004945878.1 |
Related articles in PubMed
Shifted Golgi targeting of glycosyltransferases and ?-mannosidase IA from giantin to GM130-GRASP65 results in formation of high mannose N-glycans in aggressive prostate cancer cells.
Bhat G, Hothpet VR, Lin MF, Cheng PW
Biochimica et biophysica acta1861(11 Pt A)2891-2901(2017 11)
Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)
Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Yachie N, Petsalaki E, Mellor JC, Weile J, Jacob Y, Verby M, Ozturk SB, Li S, Cote AG, Mosca R, Knapp JJ, Ko M, Yu A, Gebbia M, Sahni N, Yi S, Tyagi T, Sheykhkarimli D, Roth JF, Wong C, Musa L, Snider J, Liu YC, Yu H, Braun P, Stagljar I, Hao T, Calderwood MA, Pelletier L, Aloy P, Hill DE, Vidal M, Roth FP
Molecular systems biology12(4)863(2016 Apr)
An inter-species protein-protein interaction network across vast evolutionary distance.
Zhong Q, Pevzner SJ, Hao T, Wang Y, Mosca R, Menche J, Taipale M, Ta?an M, Fan C, Yang X, Haley P, Murray RR, Mer F, Gebreab F, Tam S, MacWilliams A, Dricot A, Reichert P, Santhanam B, Ghamsari L, Calderwood MA, Rolland T, Charloteaux B, Lindquist S, Barab?si AL, Hill DE, Aloy P, Cusick ME, Xia Y, Roth FP, Vidal M
Molecular systems biology12(4)865(2016 Apr)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
We describe the first human patient with a homozygous apparently loss of function mutation in GOLGA2. The phenotype is a neuromuscular disorder characterized by developmental delay, seizures, progressive microcephaly, and muscular dystrophy.
Title: GOLGA2, encoding a master regulator of golgi apparatus, is mutated in a patient with a neuromuscular disorder.
Data show that WAC directly binds to GM130 and that this binding is required for autophagosome formation through interacting with GABARAP regulating its subcellular localization.
Title: Activation of ULK Kinase and Autophagy by GABARAP Trafficking from the Centrosome Is Regulated by WAC and GM130.
Mutagenesis experiments support these structural observations and demonstrate that they are required for GRASP65-GM130 association.
Title: Structural basis for the interaction between the Golgi reassembly-stacking protein GRASP65 and the Golgi matrix protein GM130.
the study identified GM130 as a novel target of Coxsackievirus B3 (CVB3), which may implicate in the pathogenesis of CVB3-induced acute pancreatitis.
Title: Identification of the interaction of VP1 with GM130 which may implicate in the pathogenesis of CVB3-induced acute pancreatitis.
GM130 upregulated expression of the key epithelial-mesenchymal transformation regulator Snail (SNAI1), which mediated EMT activation and cell invasion by GM130.
Title: GM130 regulates epithelial-to-mesenchymal transition and invasion of gastric cancer cells via snail.
The following GOLGA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GOLGA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OHu04398 |
|
| Clone ID Related Accession (Same CDS sequence) | NM_004486.4 | |
| Accession Version | NM_004486.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3009bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-11-26 | |
| Organism | Homo sapiens(human) | |
| Product | golgin subfamily A member 2 | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC069268.1. On May 7, 2004 this sequence version replaced NM_004486.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC069268.1, SRR1660805.115790.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGTGGCCCC AACCCCGCCT CCCTCCCCGC CCCGCGATGT CGGAAGAAAC CCGACAGAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_004477.3 |
| CDS | 14..3022 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_004486.4 |
ORF Insert Sequence:
1 | ATGTGGCCCC AACCCCGCCT CCCTCCCCGC CCCGCGATGT CGGAAGAAAC CCGACAGAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu43204 |
|
| Clone ID Related Accession (Same CDS sequence) | XM_005251930.1 | |
| Accession Version | XM_005251930.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-05 | |
| Organism | Homo sapiens(human) | |
| Product | golgin subfamily A member 2 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGTGGCCCC AACCCCGCCT CCCTCCCCGC CCCGCGATGT CGGAAGAAAC CCGACAGAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005251987.1 |
| CDS | 32..3121 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005251930.1 |
ORF Insert Sequence:
1 | ATGTGGCCCC AACCCCGCCT CCCTCCCCGC CCCGCGATGT CGGAAGAAAC CCGACAGAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu107612 | |
| Clone ID Related Accession (Same CDS sequence) | NM_004486.5 | |
| Accession Version | NM_004486.5 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2973bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-09 | |
| Organism | Homo sapiens(human) | |
| Product | golgin subfamily A member 2 isoform 2 | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC069268.1. On Sep 27, 2018 this sequence version replaced NM_004486.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC069268.1, SRR7346977.2158357.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGTCGGAAG AAACCCGACA GAGCAAATTG GCCGCAGCGA AGAAAAAGTT GAGAGAATAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_004477.4 |
| CDS | 13..2985 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_004486.5 |
ORF Insert Sequence:
1 | ATGTCGGAAG AAACCCGACA GAGCAAATTG GCCGCAGCGA AGAAAAAGTT GAGAGAATAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu107626 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001366246.1 | |
| Accession Version | NM_001366246.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2973bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-09 | |
| Organism | Homo sapiens(human) | |
| Product | golgin subfamily A member 2 isoform 3 | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL590708.18. On Sep 27, 2018 this sequence version replaced XM_005251931.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.18808.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGTCGGAAG AAACCCGACA GAGCAAATTG GCCGCAGCGA AGAAAAAGTT GAGAGAATAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001353175.1 |
| CDS | 13..2985 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001366246.1 |
ORF Insert Sequence:
1 | ATGTCGGAAG AAACCCGACA GAGCAAATTG GCCGCAGCGA AGAAAAAGTT GAGAGAATAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu107829 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001366244.1 | |
| Accession Version | NM_001366244.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-10 | |
| Organism | Homo sapiens(human) | |
| Product | golgin subfamily A member 2 isoform 1 | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL590708.18. On Sep 27, 2018 this sequence version replaced XM_005251930.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.221892.1, SRR1660807.3720.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGTCGGAAG AAACCCGACA GAGCAAATTG GCCGCAGCGA AGAAAAAGTT GAGAGAATAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001353173.1 |
| CDS | 13..3066 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001366244.1 |
ORF Insert Sequence:
1 | ATGTCGGAAG AAACCCGACA GAGCAAATTG GCCGCAGCGA AGAAAAAGTT GAGAGAATAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu43205 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005251931.1 | |
| Accession Version | XM_005251931.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3009bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-05 | |
| Organism | Homo sapiens(human) | |
| Product | golgin subfamily A member 2 isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGTGGCCCC AACCCCGCCT CCCTCCCCGC CCCGCGATGT CGGAAGAAAC CCGACAGAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005251988.1 |
| CDS | 32..3040 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005251931.1 |
ORF Insert Sequence:
1 | ATGTGGCCCC AACCCCGCCT CCCTCCCCGC CCCGCGATGT CGGAAGAAAC CCGACAGAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu43206 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005251932.1 | |
| Accession Version | XM_005251932.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-05 | |
| Organism | Homo sapiens(human) | |
| Product | golgin subfamily A member 2 isoform X3 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGTGGCCCC AACCCCGCCT CCCTCCCCGC CCCGCGATGT CGGAAGAAAC CCGACAGAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005251989.1 |
| CDS | 25..2952 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005251932.1 |
ORF Insert Sequence:
1 | ATGTGGCCCC AACCCCGCCT CCCTCCCCGC CCCGCGATGT CGGAAGAAAC CCGACAGAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
 |
Shifted Golgi targeting of glycosyltransferases and ?-mannosidase IA from giantin to GM130-GRASP65 results in formation of high mannose N-glycans in aggressive prostate cancer cells. |
|
Architecture of the human interactome defines protein communities and disease networks. |
|
Pooled-matrix protein interaction screens using Barcode Fusion Genetics. |
|
An inter-species protein-protein interaction network across vast evolutionary distance. |