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ARSA cDNA ORF clone, Homo sapiens (human)

Gene Symbol ARSA
Entrez Gene ID 410
Full Name arylsulfatase A
Synonyms MLD
General protein information
Preferred Names
arylsulfatase A
Names
arylsulfatase A
ASA
cerebroside-sulfatase
NP_000478.3
NP_001078894.2
NP_001078895.2
NP_001078896.2
NP_001078897.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

22

22q13.33

Summary The protein encoded by this gene hydrolyzes cerebroside sulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Dec 2010]. lac of sum
Disorder MIM:

607574

Disorder Html: Metachromatic leukodystrophy, 250100 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_011530690 XP_011528992 arylsulfatase A isoform X1
XM_011530691 XP_011528993 arylsulfatase A isoform X2
NM_000487 NP_000478 arylsulfatase A isoform a precursor
NM_001085425 NP_001078894 arylsulfatase A isoform a precursor
NM_001085426 NP_001078895 arylsulfatase A isoform a precursor
NM_001085428 NP_001078897 arylsulfatase A isoform b
NM_001085427 NP_001078896 arylsulfatase A isoform a precursor



Homo sapiens (human) ARSA NP_000478.3
Pan troglodytes (chimpanzee) ARSA XP_003317377.1
Macaca mulatta (Rhesus monkey) ARSA XP_001113032.1
Canis lupus familiaris (dog) ARSA NP_001041548.1
Bos taurus (cattle) ARSA NP_001068673.1
Mus musculus (house mouse) Arsa NP_033843.2
Rattus norvegicus (Norway rat) Arsa NP_001030105.2
Gallus gallus (chicken) ARSA XP_424471.3
Danio rerio (zebrafish) arsa NP_001013561.2
Xenopus (Silurana) tropicalis (western clawed frog) LOC100487749 XP_002941879.1
Xenopus (Silurana) tropicalis (western clawed frog) arsa XP_002938337.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ARSA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARSA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu23221 XM_011530690 PREDICTED: Homo sapiens arylsulfatase A (ARSA), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu56370 XM_011530691 PREDICTED: Homo sapiens arylsulfatase A (ARSA), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu22035
NM_000487 Homo sapiens arylsulfatase A (ARSA), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu22035
NM_001085425 Homo sapiens arylsulfatase A (ARSA), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu22035
NM_001085426 Homo sapiens arylsulfatase A (ARSA), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu23221 NM_001085428 Homo sapiens arylsulfatase A (ARSA), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu22035
NM_001085427 Homo sapiens arylsulfatase A (ARSA), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu23221
Clone ID Related Accession (Same CDS sequence) NM_001085428 , XM_011530690
Accession Version XM_011530690.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1272bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product arylsulfatase A isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011526.8) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGGCATGT ACCCTGGCGT CCTGGTGCCC AGCTCCCGGG GGGGCCTGCC CCTGGAGGAG 
GTGACCGTGG CCGAAGTCCT GGCTGCCCGA GGCTACCTCA CAGGAATGGC CGGCAAGTGG
CACCTTGGGG TGGGGCCTGA GGGGGCCTTC CTGCCCCCCC ATCAGGGCTT CCATCGATTT
CTAGGCATCC CGTACTCCCA CGACCAGGGC CCCTGCCAGA ACCTGACCTG CTTCCCGCCG
GCCACTCCTT GCGACGGTGG CTGTGACCAG GGCCTGGTCC CCATCCCACT GTTGGCCAAC
CTGTCCGTGG AGGCGCAGCC CCCCTGGCTG CCCGGACTAG AGGCCCGCTA CATGGCTTTC
GCCCATGACC TCATGGCCGA CGCCCAGCGC CAGGATCGCC CCTTCTTCCT GTACTATGCC
TCTCACCACA CCCACTACCC TCAGTTCAGT GGGCAGAGCT TTGCAGAGCG TTCAGGCCGC
GGGCCATTTG GGGACTCCCT GATGGAGCTG GATGCAGCTG TGGGGACCCT GATGACAGCC
ATAGGGGACC TGGGGCTGCT TGAAGAGACG CTGGTCATCT TCACTGCAGA CAATGGACCT
GAGACCATGC GTATGTCCCG AGGCGGCTGC TCCGGTCTCT TGCGGTGTGG AAAGGGAACG
ACCTACGAGG GCGGTGTCCG AGAGCCTGCC TTGGCCTTCT GGCCAGGTCA TATCGCTCCC
GGCGTGACCC ACGAGCTGGC CAGCTCCCTG GACCTGCTGC CTACCCTGGC AGCCCTGGCT
GGGGCCCCAC TGCCCAATGT CACCTTGGAT GGCTTTGACC TCAGCCCCCT GCTGCTGGGC
ACAGGCAAGA GCCCTCGGCA GTCTCTCTTC TTCTACCCGT CCTACCCAGA CGAGGTCCGT
GGGGTTTTTG CTGTGCGGAC TGGAAAGTAC AAGGCTCACT TCTTCACCCA GGGCTCTGCC
CACAGTGATA CCACTGCAGA CCCTGCCTGC CACGCCTCCA GCTCTCTGAC TGCTCATGAG
CCCCCGCTGC TCTATGACCT GTCCAAGGAC CCTGGTGAGA ACTACAACCT GCTGGGGGGT
GTGGCCGGGG CCACCCCAGA GGTGCTGCAA GCCCTGAAAC AGCTTCAGCT GCTCAAGGCC
CAGTTAGACG CAGCTGTGAC CTTCGGCCCC AGCCAGGTGG CCCGGGGCGA GGACCCCGCC
CTGCAGATCT GCTGTCATCC TGGCTGCACC CCCCGCCCAG CTTGCTGCCA TTGCCCAGAT
CCCCATGCCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011528992.1
CDS217..1488
Misc Feature(1)217..1287(+)
Misc Feature(2)241..1002(+)
Misc Feature(3)1066..1470(+)
Translation

Target ORF information:

RefSeq Version XM_011530690
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens arylsulfatase A (ARSA), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011530690

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGGCATGT ACCCTGGCGT CCTGGTGCCC AGCTCCCGGG GGGGCCTGCC CCTGGAGGAG 
GTGACCGTGG CCGAAGTCCT GGCTGCCCGA GGCTACCTCA CAGGAATGGC CGGCAAGTGG
CACCTTGGGG TGGGGCCTGA GGGGGCCTTC CTGCCCCCCC ATCAGGGCTT CCATCGATTT
CTAGGCATCC CGTACTCCCA CGACCAGGGC CCCTGCCAGA ACCTGACCTG CTTCCCGCCG
GCCACTCCTT GCGACGGTGG CTGTGACCAG GGCCTGGTCC CCATCCCACT GTTGGCCAAC
CTGTCCGTGG AGGCGCAGCC CCCCTGGCTG CCCGGACTAG AGGCCCGCTA CATGGCTTTC
GCCCATGACC TCATGGCCGA CGCCCAGCGC CAGGATCGCC CCTTCTTCCT GTACTATGCC
TCTCACCACA CCCACTACCC TCAGTTCAGT GGGCAGAGCT TTGCAGAGCG TTCAGGCCGC
GGGCCATTTG GGGACTCCCT GATGGAGCTG GATGCAGCTG TGGGGACCCT GATGACAGCC
ATAGGGGACC TGGGGCTGCT TGAAGAGACG CTGGTCATCT TCACTGCAGA CAATGGACCT
GAGACCATGC GTATGTCCCG AGGCGGCTGC TCCGGTCTCT TGCGGTGTGG AAAGGGAACG
ACCTACGAGG GCGGTGTCCG AGAGCCTGCC TTGGCCTTCT GGCCAGGTCA TATCGCTCCC
GGCGTGACCC ACGAGCTGGC CAGCTCCCTG GACCTGCTGC CTACCCTGGC AGCCCTGGCT
GGGGCCCCAC TGCCCAATGT CACCTTGGAT GGCTTTGACC TCAGCCCCCT GCTGCTGGGC
ACAGGCAAGA GCCCTCGGCA GTCTCTCTTC TTCTACCCGT CCTACCCAGA CGAGGTCCGT
GGGGTTTTTG CTGTGCGGAC TGGAAAGTAC AAGGCTCACT TCTTCACCCA GGGCTCTGCC
CACAGTGATA CCACTGCAGA CCCTGCCTGC CACGCCTCCA GCTCTCTGAC TGCTCATGAG
CCCCCGCTGC TCTATGACCT GTCCAAGGAC CCTGGTGAGA ACTACAACCT GCTGGGGGGT
GTGGCCGGGG CCACCCCAGA GGTGCTGCAA GCCCTGAAAC AGCTTCAGCT GCTCAAGGCC
CAGTTAGACG CAGCTGTGAC CTTCGGCCCC AGCCAGGTGG CCCGGGGCGA GGACCCCGCC
CTGCAGATCT GCTGTCATCC TGGCTGCACC CCCCGCCCAG CTTGCTGCCA TTGCCCAGAT
CCCCATGCCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56370
Clone ID Related Accession (Same CDS sequence) XM_011530691
Accession Version XM_011530691.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1164bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product arylsulfatase A isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011526.8) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGTCCATGG GGGCACCGCG GTCCCTCCTC CTGGCCCTGG CTGCTGGCCT GGCCGTTGCC 
CGTCCGCCCA ACATCGTGCT GATCTTTGCC GACGACCTCG GCTATGGGGA CCTGGGCTGC
TATGGGCACC CCAGCTCTAC CACTCCCAAC CTGGACCAGC TGGCGGCGGG AGGGCTGCGG
TTCACAGACT TCTACGTGCC TGTGTCTCTG TGCACACCCT CTAGGGCCGC CCTCCTGACC
GGCCGGCTCC CGGTTCGGAT GGGCATGTAC CCTGGCGTCC TGGTGCCCAG CTCCCGGGGG
GGCCTGCCCC TGGAGGAGGT GACCGTGGCC GAAGTCCTGG CTGCCCGAGG CTACCTCACA
GGAATGGCCG GCAAGTGGCA CCTTGGGGTG GGGCCTGAGG GGGCCTTCCT GCCCCCCCAT
CAGGGCTTCC ATCGATTTCT AGGCATCCCG TACTCCCACG ACCAGGGCCC CTGCCAGAAC
CTGACCTGCT TCCCGCCGGC CACTCCTTGC GACGGTGGCT GTGACCAGGG CCTGGTCCCC
ATCCCACTGT TGGCCAACCT GTCCGTGGAG GCGCAGCCCC CCTGGCTGCC CGGACTAGAG
GCCCGCTACA TGGCTTTCGC CCATGACCTC ATGGCCGACG CCCAGCGCCA GGATCGCCCC
TTCTTCCTGT ACTATGCCTC TCACCACACC CACTACCCTC AGTTCAGTGG GCAGAGCTTT
GCAGAGCGTT CAGGCCGCGG GCCATTTGGG GACTCCCTGA TGGAGCTGGA TGCAGCTGTG
GGGACCCTGA TGACAGCCAT AGGGGACCTG GGGCTGCTTG AAGAGACGCT GGTCATCTTC
ACTGCAGACA ATGGACCTGA GACCATGCGT ATGTCCCGAG GCGGCTGCTC CGGTCTCTTG
CGGTGTGGAA AGGGAACGAC CTACGAGGGC GGTGTCCGAG AGCCTGCCTT GGCCTTCTGG
CCAGGTCATA TCGCTCCCGG CGTGACCCAC GAGCTGGCCA GCTCCCTGGA CCTGCTGCCT
ACCCTGGCAG CCCTGGCTGG GGCCCCACTG CCCAATGTCA CCTTGGATGG CTTTGACCTC
AGCCCCCTGC TGCTGGGCAC AGGCAAGGCT CTGCCCACAG TGATACCACT GCAGACCCTG
CCTGCCACGC CTCCAGCTCT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011528993.1
CDS373..1536
Misc Feature(1)433..>1476(+)
Misc Feature(2)439..1416(+)
Translation

Target ORF information:

RefSeq Version XM_011530691
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens arylsulfatase A (ARSA), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011530691

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGTCCATGG GGGCACCGCG GTCCCTCCTC CTGGCCCTGG CTGCTGGCCT GGCCGTTGCC 
CGTCCGCCCA ACATCGTGCT GATCTTTGCC GACGACCTCG GCTATGGGGA CCTGGGCTGC
TATGGGCACC CCAGCTCTAC CACTCCCAAC CTGGACCAGC TGGCGGCGGG AGGGCTGCGG
TTCACAGACT TCTACGTGCC TGTGTCTCTG TGCACACCCT CTAGGGCCGC CCTCCTGACC
GGCCGGCTCC CGGTTCGGAT GGGCATGTAC CCTGGCGTCC TGGTGCCCAG CTCCCGGGGG
GGCCTGCCCC TGGAGGAGGT GACCGTGGCC GAAGTCCTGG CTGCCCGAGG CTACCTCACA
GGAATGGCCG GCAAGTGGCA CCTTGGGGTG GGGCCTGAGG GGGCCTTCCT GCCCCCCCAT
CAGGGCTTCC ATCGATTTCT AGGCATCCCG TACTCCCACG ACCAGGGCCC CTGCCAGAAC
CTGACCTGCT TCCCGCCGGC CACTCCTTGC GACGGTGGCT GTGACCAGGG CCTGGTCCCC
ATCCCACTGT TGGCCAACCT GTCCGTGGAG GCGCAGCCCC CCTGGCTGCC CGGACTAGAG
GCCCGCTACA TGGCTTTCGC CCATGACCTC ATGGCCGACG CCCAGCGCCA GGATCGCCCC
TTCTTCCTGT ACTATGCCTC TCACCACACC CACTACCCTC AGTTCAGTGG GCAGAGCTTT
GCAGAGCGTT CAGGCCGCGG GCCATTTGGG GACTCCCTGA TGGAGCTGGA TGCAGCTGTG
GGGACCCTGA TGACAGCCAT AGGGGACCTG GGGCTGCTTG AAGAGACGCT GGTCATCTTC
ACTGCAGACA ATGGACCTGA GACCATGCGT ATGTCCCGAG GCGGCTGCTC CGGTCTCTTG
CGGTGTGGAA AGGGAACGAC CTACGAGGGC GGTGTCCGAG AGCCTGCCTT GGCCTTCTGG
CCAGGTCATA TCGCTCCCGG CGTGACCCAC GAGCTGGCCA GCTCCCTGGA CCTGCTGCCT
ACCCTGGCAG CCCTGGCTGG GGCCCCACTG CCCAATGTCA CCTTGGATGG CTTTGACCTC
AGCCCCCTGC TGCTGGGCAC AGGCAAGGCT CTGCCCACAG TGATACCACT GCAGACCCTG
CCTGCCACGC CTCCAGCTCT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22035
Clone ID Related Accession (Same CDS sequence) NM_000487 , NM_001085425 , NM_001085426 , NM_001085427
Accession Version NM_000487.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1530bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product arylsulfatase A isoform a precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB028013.1, AK301098.1, AB448736.1, U62317.2 and BQ184813.1. This sequence is a reference standard in the RefSeqGene project. On Dec 4, 2010 this sequence version replaced gi:146229325. Transcript Variant: This variant (1) represents the longest transcript and it encodes the longer protein (isoform a). Variants 1, 2, 3, and 4 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB448736.1, BC014210.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962337 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGTCCATGG GGGCACCGCG GTCCCTCCTC CTGGCCCTGG CTGCTGGCCT GGCCGTTGCC 
CGTCCGCCCA ACATCGTGCT GATCTTTGCC GACGACCTCG GCTATGGGGA CCTGGGCTGC
TATGGGCACC CCAGCTCTAC CACTCCCAAC CTGGACCAGC TGGCGGCGGG AGGGCTGCGG
TTCACAGACT TCTACGTGCC TGTGTCTCTG TGCACACCCT CTAGGGCCGC CCTCCTGACC
GGCCGGCTCC CGGTTCGGAT GGGCATGTAC CCTGGCGTCC TGGTGCCCAG CTCCCGGGGG
GGCCTGCCCC TGGAGGAGGT GACCGTGGCC GAAGTCCTGG CTGCCCGAGG CTACCTCACA
GGAATGGCCG GCAAGTGGCA CCTTGGGGTG GGGCCTGAGG GGGCCTTCCT GCCCCCCCAT
CAGGGCTTCC ATCGATTTCT AGGCATCCCG TACTCCCACG ACCAGGGCCC CTGCCAGAAC
CTGACCTGCT TCCCGCCGGC CACTCCTTGC GACGGTGGCT GTGACCAGGG CCTGGTCCCC
ATCCCACTGT TGGCCAACCT GTCCGTGGAG GCGCAGCCCC CCTGGCTGCC CGGACTAGAG
GCCCGCTACA TGGCTTTCGC CCATGACCTC ATGGCCGACG CCCAGCGCCA GGATCGCCCC
TTCTTCCTGT ACTATGCCTC TCACCACACC CACTACCCTC AGTTCAGTGG GCAGAGCTTT
GCAGAGCGTT CAGGCCGCGG GCCATTTGGG GACTCCCTGA TGGAGCTGGA TGCAGCTGTG
GGGACCCTGA TGACAGCCAT AGGGGACCTG GGGCTGCTTG AAGAGACGCT GGTCATCTTC
ACTGCAGACA ATGGACCTGA GACCATGCGT ATGTCCCGAG GCGGCTGCTC CGGTCTCTTG
CGGTGTGGAA AGGGAACGAC CTACGAGGGC GGTGTCCGAG AGCCTGCCTT GGCCTTCTGG
CCAGGTCATA TCGCTCCCGG CGTGACCCAC GAGCTGGCCA GCTCCCTGGA CCTGCTGCCT
ACCCTGGCAG CCCTGGCTGG GGCCCCACTG CCCAATGTCA CCTTGGATGG CTTTGACCTC
AGCCCCCTGC TGCTGGGCAC AGGCAAGAGC CCTCGGCAGT CTCTCTTCTT CTACCCGTCC
TACCCAGACG AGGTCCGTGG GGTTTTTGCT GTGCGGACTG GAAAGTACAA GGCTCACTTC
TTCACCCAGG GCTCTGCCCA CAGTGATACC ACTGCAGACC CTGCCTGCCA CGCCTCCAGC
TCTCTGACTG CTCATGAGCC CCCGCTGCTC TATGACCTGT CCAAGGACCC TGGTGAGAAC
TACAACCTGC TGGGGGGTGT GGCCGGGGCC ACCCCAGAGG TGCTGCAAGC CCTGAAACAG
CTTCAGCTGC TCAAGGCCCA GTTAGACGCA GCTGTGACCT TCGGCCCCAG CCAGGTGGCC
CGGGGCGAGG ACCCCGCCCT GCAGATCTGC TGTCATCCTG GCTGCACCCC CCGCCCAGCT
TGCTGCCATT GCCCAGATCC CCATGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000478.3
CDS395..1924
Misc Feature(1)359..361(+)
Misc Feature(2)455..1723(+)
Misc Feature(3)461..1438(+)
Misc Feature(4)1502..1906(+)
Exon (1)1..618
Gene:ARSA
Gene Synonym:
Exon (2)619..859
Gene:ARSA
Gene Synonym:
Exon (3)860..1078
Gene:ARSA
Gene Synonym:
Exon (4)1079..1248
Gene:ARSA
Gene Synonym:
Exon (5)1249..1373
Gene:ARSA
Gene Synonym:
Exon (6)1374..1501
Gene:ARSA
Gene Synonym:
Exon (7)1502..1604
Gene:ARSA
Gene Synonym:
Exon (8)1605..4315
Gene:ARSA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000487
Organism Homo sapiens (human)
Definition Homo sapiens arylsulfatase A (ARSA), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000487

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGTCCATGG GGGCACCGCG GTCCCTCCTC CTGGCCCTGG CTGCTGGCCT GGCCGTTGCC 
CGTCCGCCCA ACATCGTGCT GATCTTTGCC GACGACCTCG GCTATGGGGA CCTGGGCTGC
TATGGGCACC CCAGCTCTAC CACTCCCAAC CTGGACCAGC TGGCGGCGGG AGGGCTGCGG
TTCACAGACT TCTACGTGCC TGTGTCTCTG TGCACACCCT CTAGGGCCGC CCTCCTGACC
GGCCGGCTCC CGGTTCGGAT GGGCATGTAC CCTGGCGTCC TGGTGCCCAG CTCCCGGGGG
GGCCTGCCCC TGGAGGAGGT GACCGTGGCC GAAGTCCTGG CTGCCCGAGG CTACCTCACA
GGAATGGCCG GCAAGTGGCA CCTTGGGGTG GGGCCTGAGG GGGCCTTCCT GCCCCCCCAT
CAGGGCTTCC ATCGATTTCT AGGCATCCCG TACTCCCACG ACCAGGGCCC CTGCCAGAAC
CTGACCTGCT TCCCGCCGGC CACTCCTTGC GACGGTGGCT GTGACCAGGG CCTGGTCCCC
ATCCCACTGT TGGCCAACCT GTCCGTGGAG GCGCAGCCCC CCTGGCTGCC CGGACTAGAG
GCCCGCTACA TGGCTTTCGC CCATGACCTC ATGGCCGACG CCCAGCGCCA GGATCGCCCC
TTCTTCCTGT ACTATGCCTC TCACCACACC CACTACCCTC AGTTCAGTGG GCAGAGCTTT
GCAGAGCGTT CAGGCCGCGG GCCATTTGGG GACTCCCTGA TGGAGCTGGA TGCAGCTGTG
GGGACCCTGA TGACAGCCAT AGGGGACCTG GGGCTGCTTG AAGAGACGCT GGTCATCTTC
ACTGCAGACA ATGGACCTGA GACCATGCGT ATGTCCCGAG GCGGCTGCTC CGGTCTCTTG
CGGTGTGGAA AGGGAACGAC CTACGAGGGC GGTGTCCGAG AGCCTGCCTT GGCCTTCTGG
CCAGGTCATA TCGCTCCCGG CGTGACCCAC GAGCTGGCCA GCTCCCTGGA CCTGCTGCCT
ACCCTGGCAG CCCTGGCTGG GGCCCCACTG CCCAATGTCA CCTTGGATGG CTTTGACCTC
AGCCCCCTGC TGCTGGGCAC AGGCAAGAGC CCTCGGCAGT CTCTCTTCTT CTACCCGTCC
TACCCAGACG AGGTCCGTGG GGTTTTTGCT GTGCGGACTG GAAAGTACAA GGCTCACTTC
TTCACCCAGG GCTCTGCCCA CAGTGATACC ACTGCAGACC CTGCCTGCCA CGCCTCCAGC
TCTCTGACTG CTCATGAGCC CCCGCTGCTC TATGACCTGT CCAAGGACCC TGGTGAGAAC
TACAACCTGC TGGGGGGTGT GGCCGGGGCC ACCCCAGAGG TGCTGCAAGC CCTGAAACAG
CTTCAGCTGC TCAAGGCCCA GTTAGACGCA GCTGTGACCT TCGGCCCCAG CCAGGTGGCC
CGGGGCGAGG ACCCCGCCCT GCAGATCTGC TGTCATCCTG GCTGCACCCC CCGCCCAGCT
TGCTGCCATT GCCCAGATCC CCATGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22035
Clone ID Related Accession (Same CDS sequence) NM_000487 , NM_001085425 , NM_001085426 , NM_001085427
Accession Version NM_001085425.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1530bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product arylsulfatase A isoform a precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB028013.1, AB448736.1, AK092752.1, U62317.2 and BQ184813.1. On Dec 4, 2010 this sequence version replaced gi:146229323. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3, and 4 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK092752.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGTCCATGG GGGCACCGCG GTCCCTCCTC CTGGCCCTGG CTGCTGGCCT GGCCGTTGCC 
CGTCCGCCCA ACATCGTGCT GATCTTTGCC GACGACCTCG GCTATGGGGA CCTGGGCTGC
TATGGGCACC CCAGCTCTAC CACTCCCAAC CTGGACCAGC TGGCGGCGGG AGGGCTGCGG
TTCACAGACT TCTACGTGCC TGTGTCTCTG TGCACACCCT CTAGGGCCGC CCTCCTGACC
GGCCGGCTCC CGGTTCGGAT GGGCATGTAC CCTGGCGTCC TGGTGCCCAG CTCCCGGGGG
GGCCTGCCCC TGGAGGAGGT GACCGTGGCC GAAGTCCTGG CTGCCCGAGG CTACCTCACA
GGAATGGCCG GCAAGTGGCA CCTTGGGGTG GGGCCTGAGG GGGCCTTCCT GCCCCCCCAT
CAGGGCTTCC ATCGATTTCT AGGCATCCCG TACTCCCACG ACCAGGGCCC CTGCCAGAAC
CTGACCTGCT TCCCGCCGGC CACTCCTTGC GACGGTGGCT GTGACCAGGG CCTGGTCCCC
ATCCCACTGT TGGCCAACCT GTCCGTGGAG GCGCAGCCCC CCTGGCTGCC CGGACTAGAG
GCCCGCTACA TGGCTTTCGC CCATGACCTC ATGGCCGACG CCCAGCGCCA GGATCGCCCC
TTCTTCCTGT ACTATGCCTC TCACCACACC CACTACCCTC AGTTCAGTGG GCAGAGCTTT
GCAGAGCGTT CAGGCCGCGG GCCATTTGGG GACTCCCTGA TGGAGCTGGA TGCAGCTGTG
GGGACCCTGA TGACAGCCAT AGGGGACCTG GGGCTGCTTG AAGAGACGCT GGTCATCTTC
ACTGCAGACA ATGGACCTGA GACCATGCGT ATGTCCCGAG GCGGCTGCTC CGGTCTCTTG
CGGTGTGGAA AGGGAACGAC CTACGAGGGC GGTGTCCGAG AGCCTGCCTT GGCCTTCTGG
CCAGGTCATA TCGCTCCCGG CGTGACCCAC GAGCTGGCCA GCTCCCTGGA CCTGCTGCCT
ACCCTGGCAG CCCTGGCTGG GGCCCCACTG CCCAATGTCA CCTTGGATGG CTTTGACCTC
AGCCCCCTGC TGCTGGGCAC AGGCAAGAGC CCTCGGCAGT CTCTCTTCTT CTACCCGTCC
TACCCAGACG AGGTCCGTGG GGTTTTTGCT GTGCGGACTG GAAAGTACAA GGCTCACTTC
TTCACCCAGG GCTCTGCCCA CAGTGATACC ACTGCAGACC CTGCCTGCCA CGCCTCCAGC
TCTCTGACTG CTCATGAGCC CCCGCTGCTC TATGACCTGT CCAAGGACCC TGGTGAGAAC
TACAACCTGC TGGGGGGTGT GGCCGGGGCC ACCCCAGAGG TGCTGCAAGC CCTGAAACAG
CTTCAGCTGC TCAAGGCCCA GTTAGACGCA GCTGTGACCT TCGGCCCCAG CCAGGTGGCC
CGGGGCGAGG ACCCCGCCCT GCAGATCTGC TGTCATCCTG GCTGCACCCC CCGCCCAGCT
TGCTGCCATT GCCCAGATCC CCATGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001078894.2
CDS290..1819
Misc Feature(1)179..181(+)
Misc Feature(2)350..1618(+)
Misc Feature(3)356..1333(+)
Misc Feature(4)1397..1801(+)
Exon (1)1..276
Gene:ARSA
Gene Synonym:
Exon (2)277..513
Gene:ARSA
Gene Synonym:
Exon (3)514..754
Gene:ARSA
Gene Synonym:
Exon (4)755..973
Gene:ARSA
Gene Synonym:
Exon (5)974..1143
Gene:ARSA
Gene Synonym:
Exon (6)1144..1268
Gene:ARSA
Gene Synonym:
Exon (7)1269..1396
Gene:ARSA
Gene Synonym:
Exon (8)1397..1499
Gene:ARSA
Gene Synonym:
Exon (9)1500..4210
Gene:ARSA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001085425
Organism Homo sapiens (human)
Definition Homo sapiens arylsulfatase A (ARSA), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001085425

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGTCCATGG GGGCACCGCG GTCCCTCCTC CTGGCCCTGG CTGCTGGCCT GGCCGTTGCC 
CGTCCGCCCA ACATCGTGCT GATCTTTGCC GACGACCTCG GCTATGGGGA CCTGGGCTGC
TATGGGCACC CCAGCTCTAC CACTCCCAAC CTGGACCAGC TGGCGGCGGG AGGGCTGCGG
TTCACAGACT TCTACGTGCC TGTGTCTCTG TGCACACCCT CTAGGGCCGC CCTCCTGACC
GGCCGGCTCC CGGTTCGGAT GGGCATGTAC CCTGGCGTCC TGGTGCCCAG CTCCCGGGGG
GGCCTGCCCC TGGAGGAGGT GACCGTGGCC GAAGTCCTGG CTGCCCGAGG CTACCTCACA
GGAATGGCCG GCAAGTGGCA CCTTGGGGTG GGGCCTGAGG GGGCCTTCCT GCCCCCCCAT
CAGGGCTTCC ATCGATTTCT AGGCATCCCG TACTCCCACG ACCAGGGCCC CTGCCAGAAC
CTGACCTGCT TCCCGCCGGC CACTCCTTGC GACGGTGGCT GTGACCAGGG CCTGGTCCCC
ATCCCACTGT TGGCCAACCT GTCCGTGGAG GCGCAGCCCC CCTGGCTGCC CGGACTAGAG
GCCCGCTACA TGGCTTTCGC CCATGACCTC ATGGCCGACG CCCAGCGCCA GGATCGCCCC
TTCTTCCTGT ACTATGCCTC TCACCACACC CACTACCCTC AGTTCAGTGG GCAGAGCTTT
GCAGAGCGTT CAGGCCGCGG GCCATTTGGG GACTCCCTGA TGGAGCTGGA TGCAGCTGTG
GGGACCCTGA TGACAGCCAT AGGGGACCTG GGGCTGCTTG AAGAGACGCT GGTCATCTTC
ACTGCAGACA ATGGACCTGA GACCATGCGT ATGTCCCGAG GCGGCTGCTC CGGTCTCTTG
CGGTGTGGAA AGGGAACGAC CTACGAGGGC GGTGTCCGAG AGCCTGCCTT GGCCTTCTGG
CCAGGTCATA TCGCTCCCGG CGTGACCCAC GAGCTGGCCA GCTCCCTGGA CCTGCTGCCT
ACCCTGGCAG CCCTGGCTGG GGCCCCACTG CCCAATGTCA CCTTGGATGG CTTTGACCTC
AGCCCCCTGC TGCTGGGCAC AGGCAAGAGC CCTCGGCAGT CTCTCTTCTT CTACCCGTCC
TACCCAGACG AGGTCCGTGG GGTTTTTGCT GTGCGGACTG GAAAGTACAA GGCTCACTTC
TTCACCCAGG GCTCTGCCCA CAGTGATACC ACTGCAGACC CTGCCTGCCA CGCCTCCAGC
TCTCTGACTG CTCATGAGCC CCCGCTGCTC TATGACCTGT CCAAGGACCC TGGTGAGAAC
TACAACCTGC TGGGGGGTGT GGCCGGGGCC ACCCCAGAGG TGCTGCAAGC CCTGAAACAG
CTTCAGCTGC TCAAGGCCCA GTTAGACGCA GCTGTGACCT TCGGCCCCAG CCAGGTGGCC
CGGGGCGAGG ACCCCGCCCT GCAGATCTGC TGTCATCCTG GCTGCACCCC CCGCCCAGCT
TGCTGCCATT GCCCAGATCC CCATGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22035
Clone ID Related Accession (Same CDS sequence) NM_000487 , NM_001085425 , NM_001085426 , NM_001085427
Accession Version NM_001085426.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1530bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product arylsulfatase A isoform a precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB028013.1, AK315011.1, AB448736.1, U62317.2 and BQ184813.1. On Dec 4, 2010 this sequence version replaced gi:146229328. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3, and 4 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX648618.1, AK315011.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGTCCATGG GGGCACCGCG GTCCCTCCTC CTGGCCCTGG CTGCTGGCCT GGCCGTTGCC 
CGTCCGCCCA ACATCGTGCT GATCTTTGCC GACGACCTCG GCTATGGGGA CCTGGGCTGC
TATGGGCACC CCAGCTCTAC CACTCCCAAC CTGGACCAGC TGGCGGCGGG AGGGCTGCGG
TTCACAGACT TCTACGTGCC TGTGTCTCTG TGCACACCCT CTAGGGCCGC CCTCCTGACC
GGCCGGCTCC CGGTTCGGAT GGGCATGTAC CCTGGCGTCC TGGTGCCCAG CTCCCGGGGG
GGCCTGCCCC TGGAGGAGGT GACCGTGGCC GAAGTCCTGG CTGCCCGAGG CTACCTCACA
GGAATGGCCG GCAAGTGGCA CCTTGGGGTG GGGCCTGAGG GGGCCTTCCT GCCCCCCCAT
CAGGGCTTCC ATCGATTTCT AGGCATCCCG TACTCCCACG ACCAGGGCCC CTGCCAGAAC
CTGACCTGCT TCCCGCCGGC CACTCCTTGC GACGGTGGCT GTGACCAGGG CCTGGTCCCC
ATCCCACTGT TGGCCAACCT GTCCGTGGAG GCGCAGCCCC CCTGGCTGCC CGGACTAGAG
GCCCGCTACA TGGCTTTCGC CCATGACCTC ATGGCCGACG CCCAGCGCCA GGATCGCCCC
TTCTTCCTGT ACTATGCCTC TCACCACACC CACTACCCTC AGTTCAGTGG GCAGAGCTTT
GCAGAGCGTT CAGGCCGCGG GCCATTTGGG GACTCCCTGA TGGAGCTGGA TGCAGCTGTG
GGGACCCTGA TGACAGCCAT AGGGGACCTG GGGCTGCTTG AAGAGACGCT GGTCATCTTC
ACTGCAGACA ATGGACCTGA GACCATGCGT ATGTCCCGAG GCGGCTGCTC CGGTCTCTTG
CGGTGTGGAA AGGGAACGAC CTACGAGGGC GGTGTCCGAG AGCCTGCCTT GGCCTTCTGG
CCAGGTCATA TCGCTCCCGG CGTGACCCAC GAGCTGGCCA GCTCCCTGGA CCTGCTGCCT
ACCCTGGCAG CCCTGGCTGG GGCCCCACTG CCCAATGTCA CCTTGGATGG CTTTGACCTC
AGCCCCCTGC TGCTGGGCAC AGGCAAGAGC CCTCGGCAGT CTCTCTTCTT CTACCCGTCC
TACCCAGACG AGGTCCGTGG GGTTTTTGCT GTGCGGACTG GAAAGTACAA GGCTCACTTC
TTCACCCAGG GCTCTGCCCA CAGTGATACC ACTGCAGACC CTGCCTGCCA CGCCTCCAGC
TCTCTGACTG CTCATGAGCC CCCGCTGCTC TATGACCTGT CCAAGGACCC TGGTGAGAAC
TACAACCTGC TGGGGGGTGT GGCCGGGGCC ACCCCAGAGG TGCTGCAAGC CCTGAAACAG
CTTCAGCTGC TCAAGGCCCA GTTAGACGCA GCTGTGACCT TCGGCCCCAG CCAGGTGGCC
CGGGGCGAGG ACCCCGCCCT GCAGATCTGC TGTCATCCTG GCTGCACCCC CCGCCCAGCT
TGCTGCCATT GCCCAGATCC CCATGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001078895.2
CDS242..1771
Misc Feature(1)179..181(+)
Misc Feature(2)302..1570(+)
Misc Feature(3)308..1285(+)
Misc Feature(4)1349..1753(+)
Exon (1)1..228
Gene:ARSA
Gene Synonym:
Exon (2)229..465
Gene:ARSA
Gene Synonym:
Exon (3)466..706
Gene:ARSA
Gene Synonym:
Exon (4)707..925
Gene:ARSA
Gene Synonym:
Exon (5)926..1095
Gene:ARSA
Gene Synonym:
Exon (6)1096..1220
Gene:ARSA
Gene Synonym:
Exon (7)1221..1348
Gene:ARSA
Gene Synonym:
Exon (8)1349..1451
Gene:ARSA
Gene Synonym:
Exon (9)1452..4162
Gene:ARSA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001085426
Organism Homo sapiens (human)
Definition Homo sapiens arylsulfatase A (ARSA), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001085426

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGTCCATGG GGGCACCGCG GTCCCTCCTC CTGGCCCTGG CTGCTGGCCT GGCCGTTGCC 
CGTCCGCCCA ACATCGTGCT GATCTTTGCC GACGACCTCG GCTATGGGGA CCTGGGCTGC
TATGGGCACC CCAGCTCTAC CACTCCCAAC CTGGACCAGC TGGCGGCGGG AGGGCTGCGG
TTCACAGACT TCTACGTGCC TGTGTCTCTG TGCACACCCT CTAGGGCCGC CCTCCTGACC
GGCCGGCTCC CGGTTCGGAT GGGCATGTAC CCTGGCGTCC TGGTGCCCAG CTCCCGGGGG
GGCCTGCCCC TGGAGGAGGT GACCGTGGCC GAAGTCCTGG CTGCCCGAGG CTACCTCACA
GGAATGGCCG GCAAGTGGCA CCTTGGGGTG GGGCCTGAGG GGGCCTTCCT GCCCCCCCAT
CAGGGCTTCC ATCGATTTCT AGGCATCCCG TACTCCCACG ACCAGGGCCC CTGCCAGAAC
CTGACCTGCT TCCCGCCGGC CACTCCTTGC GACGGTGGCT GTGACCAGGG CCTGGTCCCC
ATCCCACTGT TGGCCAACCT GTCCGTGGAG GCGCAGCCCC CCTGGCTGCC CGGACTAGAG
GCCCGCTACA TGGCTTTCGC CCATGACCTC ATGGCCGACG CCCAGCGCCA GGATCGCCCC
TTCTTCCTGT ACTATGCCTC TCACCACACC CACTACCCTC AGTTCAGTGG GCAGAGCTTT
GCAGAGCGTT CAGGCCGCGG GCCATTTGGG GACTCCCTGA TGGAGCTGGA TGCAGCTGTG
GGGACCCTGA TGACAGCCAT AGGGGACCTG GGGCTGCTTG AAGAGACGCT GGTCATCTTC
ACTGCAGACA ATGGACCTGA GACCATGCGT ATGTCCCGAG GCGGCTGCTC CGGTCTCTTG
CGGTGTGGAA AGGGAACGAC CTACGAGGGC GGTGTCCGAG AGCCTGCCTT GGCCTTCTGG
CCAGGTCATA TCGCTCCCGG CGTGACCCAC GAGCTGGCCA GCTCCCTGGA CCTGCTGCCT
ACCCTGGCAG CCCTGGCTGG GGCCCCACTG CCCAATGTCA CCTTGGATGG CTTTGACCTC
AGCCCCCTGC TGCTGGGCAC AGGCAAGAGC CCTCGGCAGT CTCTCTTCTT CTACCCGTCC
TACCCAGACG AGGTCCGTGG GGTTTTTGCT GTGCGGACTG GAAAGTACAA GGCTCACTTC
TTCACCCAGG GCTCTGCCCA CAGTGATACC ACTGCAGACC CTGCCTGCCA CGCCTCCAGC
TCTCTGACTG CTCATGAGCC CCCGCTGCTC TATGACCTGT CCAAGGACCC TGGTGAGAAC
TACAACCTGC TGGGGGGTGT GGCCGGGGCC ACCCCAGAGG TGCTGCAAGC CCTGAAACAG
CTTCAGCTGC TCAAGGCCCA GTTAGACGCA GCTGTGACCT TCGGCCCCAG CCAGGTGGCC
CGGGGCGAGG ACCCCGCCCT GCAGATCTGC TGTCATCCTG GCTGCACCCC CCGCCCAGCT
TGCTGCCATT GCCCAGATCC CCATGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23221
Clone ID Related Accession (Same CDS sequence) NM_001085428 , XM_011530690
Accession Version NM_001085428.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1272bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product arylsulfatase A isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB028013.1, AB448736.1, U62317.2 and BQ184813.1. On or before Dec 5, 2010 this sequence version replaced gi:310114078, gi:310133113, gi:310124166, gi:146229326. Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK098659.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962337 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGGCATGT ACCCTGGCGT CCTGGTGCCC AGCTCCCGGG GGGGCCTGCC CCTGGAGGAG 
GTGACCGTGG CCGAAGTCCT GGCTGCCCGA GGCTACCTCA CAGGAATGGC CGGCAAGTGG
CACCTTGGGG TGGGGCCTGA GGGGGCCTTC CTGCCCCCCC ATCAGGGCTT CCATCGATTT
CTAGGCATCC CGTACTCCCA CGACCAGGGC CCCTGCCAGA ACCTGACCTG CTTCCCGCCG
GCCACTCCTT GCGACGGTGG CTGTGACCAG GGCCTGGTCC CCATCCCACT GTTGGCCAAC
CTGTCCGTGG AGGCGCAGCC CCCCTGGCTG CCCGGACTAG AGGCCCGCTA CATGGCTTTC
GCCCATGACC TCATGGCCGA CGCCCAGCGC CAGGATCGCC CCTTCTTCCT GTACTATGCC
TCTCACCACA CCCACTACCC TCAGTTCAGT GGGCAGAGCT TTGCAGAGCG TTCAGGCCGC
GGGCCATTTG GGGACTCCCT GATGGAGCTG GATGCAGCTG TGGGGACCCT GATGACAGCC
ATAGGGGACC TGGGGCTGCT TGAAGAGACG CTGGTCATCT TCACTGCAGA CAATGGACCT
GAGACCATGC GTATGTCCCG AGGCGGCTGC TCCGGTCTCT TGCGGTGTGG AAAGGGAACG
ACCTACGAGG GCGGTGTCCG AGAGCCTGCC TTGGCCTTCT GGCCAGGTCA TATCGCTCCC
GGCGTGACCC ACGAGCTGGC CAGCTCCCTG GACCTGCTGC CTACCCTGGC AGCCCTGGCT
GGGGCCCCAC TGCCCAATGT CACCTTGGAT GGCTTTGACC TCAGCCCCCT GCTGCTGGGC
ACAGGCAAGA GCCCTCGGCA GTCTCTCTTC TTCTACCCGT CCTACCCAGA CGAGGTCCGT
GGGGTTTTTG CTGTGCGGAC TGGAAAGTAC AAGGCTCACT TCTTCACCCA GGGCTCTGCC
CACAGTGATA CCACTGCAGA CCCTGCCTGC CACGCCTCCA GCTCTCTGAC TGCTCATGAG
CCCCCGCTGC TCTATGACCT GTCCAAGGAC CCTGGTGAGA ACTACAACCT GCTGGGGGGT
GTGGCCGGGG CCACCCCAGA GGTGCTGCAA GCCCTGAAAC AGCTTCAGCT GCTCAAGGCC
CAGTTAGACG CAGCTGTGAC CTTCGGCCCC AGCCAGGTGG CCCGGGGCGA GGACCCCGCC
CTGCAGATCT GCTGTCATCC TGGCTGCACC CCCCGCCCAG CTTGCTGCCA TTGCCCAGAT
CCCCATGCCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001078897.1
CDS263..1534
Misc Feature(1)179..181(+)
Misc Feature(2)263..1333(+)
Misc Feature(3)287..1048(+)
Misc Feature(4)1112..1516(+)
Exon (1)1..228
Gene:ARSA
Gene Synonym:
Exon (2)229..469
Gene:ARSA
Gene Synonym:
Exon (3)470..688
Gene:ARSA
Gene Synonym:
Exon (4)689..858
Gene:ARSA
Gene Synonym:
Exon (5)859..983
Gene:ARSA
Gene Synonym:
Exon (6)984..1111
Gene:ARSA
Gene Synonym:
Exon (7)1112..1214
Gene:ARSA
Gene Synonym:
Exon (8)1215..3925
Gene:ARSA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001085428
Organism Homo sapiens (human)
Definition Homo sapiens arylsulfatase A (ARSA), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001085428

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGGCATGT ACCCTGGCGT CCTGGTGCCC AGCTCCCGGG GGGGCCTGCC CCTGGAGGAG 
GTGACCGTGG CCGAAGTCCT GGCTGCCCGA GGCTACCTCA CAGGAATGGC CGGCAAGTGG
CACCTTGGGG TGGGGCCTGA GGGGGCCTTC CTGCCCCCCC ATCAGGGCTT CCATCGATTT
CTAGGCATCC CGTACTCCCA CGACCAGGGC CCCTGCCAGA ACCTGACCTG CTTCCCGCCG
GCCACTCCTT GCGACGGTGG CTGTGACCAG GGCCTGGTCC CCATCCCACT GTTGGCCAAC
CTGTCCGTGG AGGCGCAGCC CCCCTGGCTG CCCGGACTAG AGGCCCGCTA CATGGCTTTC
GCCCATGACC TCATGGCCGA CGCCCAGCGC CAGGATCGCC CCTTCTTCCT GTACTATGCC
TCTCACCACA CCCACTACCC TCAGTTCAGT GGGCAGAGCT TTGCAGAGCG TTCAGGCCGC
GGGCCATTTG GGGACTCCCT GATGGAGCTG GATGCAGCTG TGGGGACCCT GATGACAGCC
ATAGGGGACC TGGGGCTGCT TGAAGAGACG CTGGTCATCT TCACTGCAGA CAATGGACCT
GAGACCATGC GTATGTCCCG AGGCGGCTGC TCCGGTCTCT TGCGGTGTGG AAAGGGAACG
ACCTACGAGG GCGGTGTCCG AGAGCCTGCC TTGGCCTTCT GGCCAGGTCA TATCGCTCCC
GGCGTGACCC ACGAGCTGGC CAGCTCCCTG GACCTGCTGC CTACCCTGGC AGCCCTGGCT
GGGGCCCCAC TGCCCAATGT CACCTTGGAT GGCTTTGACC TCAGCCCCCT GCTGCTGGGC
ACAGGCAAGA GCCCTCGGCA GTCTCTCTTC TTCTACCCGT CCTACCCAGA CGAGGTCCGT
GGGGTTTTTG CTGTGCGGAC TGGAAAGTAC AAGGCTCACT TCTTCACCCA GGGCTCTGCC
CACAGTGATA CCACTGCAGA CCCTGCCTGC CACGCCTCCA GCTCTCTGAC TGCTCATGAG
CCCCCGCTGC TCTATGACCT GTCCAAGGAC CCTGGTGAGA ACTACAACCT GCTGGGGGGT
GTGGCCGGGG CCACCCCAGA GGTGCTGCAA GCCCTGAAAC AGCTTCAGCT GCTCAAGGCC
CAGTTAGACG CAGCTGTGAC CTTCGGCCCC AGCCAGGTGG CCCGGGGCGA GGACCCCGCC
CTGCAGATCT GCTGTCATCC TGGCTGCACC CCCCGCCCAG CTTGCTGCCA TTGCCCAGAT
CCCCATGCCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22035
Clone ID Related Accession (Same CDS sequence) NM_000487 , NM_001085425 , NM_001085426 , NM_001085427
Accession Version NM_001085427.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1530bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product arylsulfatase A isoform a precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB028013.1, U62317.2 and BQ184813.1. On Dec 4, 2010 this sequence version replaced gi:146229330. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3, and 4 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BX648618.1, X52151.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGTCCATGG GGGCACCGCG GTCCCTCCTC CTGGCCCTGG CTGCTGGCCT GGCCGTTGCC 
CGTCCGCCCA ACATCGTGCT GATCTTTGCC GACGACCTCG GCTATGGGGA CCTGGGCTGC
TATGGGCACC CCAGCTCTAC CACTCCCAAC CTGGACCAGC TGGCGGCGGG AGGGCTGCGG
TTCACAGACT TCTACGTGCC TGTGTCTCTG TGCACACCCT CTAGGGCCGC CCTCCTGACC
GGCCGGCTCC CGGTTCGGAT GGGCATGTAC CCTGGCGTCC TGGTGCCCAG CTCCCGGGGG
GGCCTGCCCC TGGAGGAGGT GACCGTGGCC GAAGTCCTGG CTGCCCGAGG CTACCTCACA
GGAATGGCCG GCAAGTGGCA CCTTGGGGTG GGGCCTGAGG GGGCCTTCCT GCCCCCCCAT
CAGGGCTTCC ATCGATTTCT AGGCATCCCG TACTCCCACG ACCAGGGCCC CTGCCAGAAC
CTGACCTGCT TCCCGCCGGC CACTCCTTGC GACGGTGGCT GTGACCAGGG CCTGGTCCCC
ATCCCACTGT TGGCCAACCT GTCCGTGGAG GCGCAGCCCC CCTGGCTGCC CGGACTAGAG
GCCCGCTACA TGGCTTTCGC CCATGACCTC ATGGCCGACG CCCAGCGCCA GGATCGCCCC
TTCTTCCTGT ACTATGCCTC TCACCACACC CACTACCCTC AGTTCAGTGG GCAGAGCTTT
GCAGAGCGTT CAGGCCGCGG GCCATTTGGG GACTCCCTGA TGGAGCTGGA TGCAGCTGTG
GGGACCCTGA TGACAGCCAT AGGGGACCTG GGGCTGCTTG AAGAGACGCT GGTCATCTTC
ACTGCAGACA ATGGACCTGA GACCATGCGT ATGTCCCGAG GCGGCTGCTC CGGTCTCTTG
CGGTGTGGAA AGGGAACGAC CTACGAGGGC GGTGTCCGAG AGCCTGCCTT GGCCTTCTGG
CCAGGTCATA TCGCTCCCGG CGTGACCCAC GAGCTGGCCA GCTCCCTGGA CCTGCTGCCT
ACCCTGGCAG CCCTGGCTGG GGCCCCACTG CCCAATGTCA CCTTGGATGG CTTTGACCTC
AGCCCCCTGC TGCTGGGCAC AGGCAAGAGC CCTCGGCAGT CTCTCTTCTT CTACCCGTCC
TACCCAGACG AGGTCCGTGG GGTTTTTGCT GTGCGGACTG GAAAGTACAA GGCTCACTTC
TTCACCCAGG GCTCTGCCCA CAGTGATACC ACTGCAGACC CTGCCTGCCA CGCCTCCAGC
TCTCTGACTG CTCATGAGCC CCCGCTGCTC TATGACCTGT CCAAGGACCC TGGTGAGAAC
TACAACCTGC TGGGGGGTGT GGCCGGGGCC ACCCCAGAGG TGCTGCAAGC CCTGAAACAG
CTTCAGCTGC TCAAGGCCCA GTTAGACGCA GCTGTGACCT TCGGCCCCAG CCAGGTGGCC
CGGGGCGAGG ACCCCGCCCT GCAGATCTGC TGTCATCCTG GCTGCACCCC CCGCCCAGCT
TGCTGCCATT GCCCAGATCC CCATGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001078896.2
CDS197..1726
Misc Feature(1)179..181(+)
Misc Feature(2)257..1525(+)
Misc Feature(3)263..1240(+)
Misc Feature(4)1304..1708(+)
Exon (1)1..183
Gene:ARSA
Gene Synonym:
Exon (2)184..420
Gene:ARSA
Gene Synonym:
Exon (3)421..661
Gene:ARSA
Gene Synonym:
Exon (4)662..880
Gene:ARSA
Gene Synonym:
Exon (5)881..1050
Gene:ARSA
Gene Synonym:
Exon (6)1051..1175
Gene:ARSA
Gene Synonym:
Exon (7)1176..1303
Gene:ARSA
Gene Synonym:
Exon (8)1304..1406
Gene:ARSA
Gene Synonym:
Exon (9)1407..4117
Gene:ARSA
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001085427
Organism Homo sapiens (human)
Definition Homo sapiens arylsulfatase A (ARSA), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001085427

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGTCCATGG GGGCACCGCG GTCCCTCCTC CTGGCCCTGG CTGCTGGCCT GGCCGTTGCC 
CGTCCGCCCA ACATCGTGCT GATCTTTGCC GACGACCTCG GCTATGGGGA CCTGGGCTGC
TATGGGCACC CCAGCTCTAC CACTCCCAAC CTGGACCAGC TGGCGGCGGG AGGGCTGCGG
TTCACAGACT TCTACGTGCC TGTGTCTCTG TGCACACCCT CTAGGGCCGC CCTCCTGACC
GGCCGGCTCC CGGTTCGGAT GGGCATGTAC CCTGGCGTCC TGGTGCCCAG CTCCCGGGGG
GGCCTGCCCC TGGAGGAGGT GACCGTGGCC GAAGTCCTGG CTGCCCGAGG CTACCTCACA
GGAATGGCCG GCAAGTGGCA CCTTGGGGTG GGGCCTGAGG GGGCCTTCCT GCCCCCCCAT
CAGGGCTTCC ATCGATTTCT AGGCATCCCG TACTCCCACG ACCAGGGCCC CTGCCAGAAC
CTGACCTGCT TCCCGCCGGC CACTCCTTGC GACGGTGGCT GTGACCAGGG CCTGGTCCCC
ATCCCACTGT TGGCCAACCT GTCCGTGGAG GCGCAGCCCC CCTGGCTGCC CGGACTAGAG
GCCCGCTACA TGGCTTTCGC CCATGACCTC ATGGCCGACG CCCAGCGCCA GGATCGCCCC
TTCTTCCTGT ACTATGCCTC TCACCACACC CACTACCCTC AGTTCAGTGG GCAGAGCTTT
GCAGAGCGTT CAGGCCGCGG GCCATTTGGG GACTCCCTGA TGGAGCTGGA TGCAGCTGTG
GGGACCCTGA TGACAGCCAT AGGGGACCTG GGGCTGCTTG AAGAGACGCT GGTCATCTTC
ACTGCAGACA ATGGACCTGA GACCATGCGT ATGTCCCGAG GCGGCTGCTC CGGTCTCTTG
CGGTGTGGAA AGGGAACGAC CTACGAGGGC GGTGTCCGAG AGCCTGCCTT GGCCTTCTGG
CCAGGTCATA TCGCTCCCGG CGTGACCCAC GAGCTGGCCA GCTCCCTGGA CCTGCTGCCT
ACCCTGGCAG CCCTGGCTGG GGCCCCACTG CCCAATGTCA CCTTGGATGG CTTTGACCTC
AGCCCCCTGC TGCTGGGCAC AGGCAAGAGC CCTCGGCAGT CTCTCTTCTT CTACCCGTCC
TACCCAGACG AGGTCCGTGG GGTTTTTGCT GTGCGGACTG GAAAGTACAA GGCTCACTTC
TTCACCCAGG GCTCTGCCCA CAGTGATACC ACTGCAGACC CTGCCTGCCA CGCCTCCAGC
TCTCTGACTG CTCATGAGCC CCCGCTGCTC TATGACCTGT CCAAGGACCC TGGTGAGAAC
TACAACCTGC TGGGGGGTGT GGCCGGGGCC ACCCCAGAGG TGCTGCAAGC CCTGAAACAG
CTTCAGCTGC TCAAGGCCCA GTTAGACGCA GCTGTGACCT TCGGCCCCAG CCAGGTGGCC
CGGGGCGAGG ACCCCGCCCT GCAGATCTGC TGTCATCCTG GCTGCACCCC CCGCCCAGCT
TGCTGCCATT GCCCAGATCC CCATGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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