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FXYD5 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol FXYD5
Entrez Gene ID 53827
Full Name FXYD domain containing ion transport regulator 5
Synonyms DYSAD,HSPC113,IWU1,KCT1,OIT2,PRO6241,RIC
General protein information
Preferred Names

FXYD domain containing ion transport regulator 5

Names

FXYD domain-containing ion transport regulator 5
dysadherin
keratinocytes associated transmembrane protein 1
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

19

19q13.12
Summary This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. This gene product, FXYD5, is a glycoprotein that functions in the up-regulation of chemokine production, and it is involved in the reduction of cell adhesion via its ability to down-regulate E-cadherin. It also promotes metastasis, and has been linked to a variety of cancers. Alternative splicing results in multiple transcript variants. [RefSeq curation by Kathleen J. Sweadner, Ph.D., [email protected], Sep 2009].
Disorder MIM:

606669


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_014164.5 NP_054883.3 FXYD domain-containing ion transport regulator 5 isoform 1 precursor
NM_014164.6 NP_054883.3 FXYD domain-containing ion transport regulator 5 isoform 1 precursor
NM_001320912.1 NP_001307841.1 FXYD domain-containing ion transport regulator 5 isoform 2 precursor
NM_001164605.1 NP_001158077.1 FXYD domain-containing ion transport regulator 5 isoform 1 precursor
NM_001320912.2 NP_001307841.1 FXYD domain-containing ion transport regulator 5 isoform 2 precursor
NM_001320913.1 NP_001307842.1 FXYD domain-containing ion transport regulator 5 isoform 3 precursor
NM_144779.2 NP_659003.1 FXYD domain-containing ion transport regulator 5 isoform 1 precursor
NM_001320913.2 NP_001307842.1 FXYD domain-containing ion transport regulator 5 isoform 3 precursor
NM_001164605.2 NP_001158077.1 FXYD domain-containing ion transport regulator 5 isoform 1 precursor
NM_144779.3 NP_659003.1 FXYD domain-containing ion transport regulator 5 isoform 1 precursor

Pathways from BioSystems


Homologies


Bos taurus (cattle) FXYD5 NP_001178992.1
Pan troglodytes (chimpanzee) FXYD5 XP_001158330.1
Canis lupus familiaris (dog) FXYD5 XP_005616778.1
Mus musculus (house mouse) Fxyd5 NP_001274146.1
LOC718467 XP_001111193.1
Homo sapiens (human) FXYD5 NP_001158077.1
Canis lupus familiaris (dog) LOC102156583 XP_005642142.1
Rattus norvegicus (Norway rat) Fxyd5 NP_068709.2

Gene Ontology


Bibliography

Related articles in PubMed

The O-glycosylated ectodomain of FXYD5 impairs adhesion by disrupting cell-cell trans-dimerization of Na,K-ATPase ?1 subunits.
Tokhtaeva E, Sun H, Deiss-Yehiely N, Wen Y, Soni PN, Gabrielli NM, Marcus EA, Ridge KM, Sachs G, Vazquez-Levin M, Sznajder JI, Vagin O, Dada LA
Journal of cell science129(12)2394-406(2016 06)

FXYD5 Protein Has a Pro-inflammatory Role in Epithelial Cells.
Lubarski-Gotliv I, Asher C, Dada LA, Garty H
The Journal of biological chemistry291(21)11072-82(2016 May)

FXYD1 negatively regulates Na(+)/K(+)-ATPase activity in lung alveolar epithelial cells.
Wujak ?A, Blume A, Balo?lu E, Wygrecka M, Wygowski J, Herold S, Mayer K, Vad?sz I, Besuch P, Mairb?url H, Seeger W, Morty RE
Respiratory physiology & neurobiology22054-61(2016 Jan)

Aberrantly regulated dysadherin and B-cell lymphoma 2/B-cell lymphoma 2-associated X enhances tumorigenesis and DNA targeting drug resistance of liver cancer stem cells.
Jiang N, Chen W, Zhang JW, Li Y, Zeng XC, Zhang T, Fu BS, Yi HM, Zhang Q
Molecular medicine reports12(5)7239-46(2015 Nov)

Modulation of cell polarization by the Na+-K+-ATPase-associated protein FXYD5 (dysadherin).
Lubarski I, Asher C, Garty H
American journal of physiology. Cell physiology306(11)C1080-8(2014 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the ratio between FXYD5 and alpha1-beta1 heterodimer determines whether the Na,K-ATPase acts as a positive or negative regulator of intercellular adhesion.
Title: The O-glycosylated ectodomain of FXYD5 impairs adhesion by disrupting cell-cell trans-dimerization of Na,K-ATPase ?1 subunits.

Knockdown of FXYD5 in MDA-MB-231 breast cancer cells largely decreases expression and secretion of the chemokine CCL2 (MCP-1). A related effect has also been observed in renal cell carcinoma cells.
Title: FXYD5 Protein Has a Pro-inflammatory Role in Epithelial Cells.

Study demonstrated that the expression of FXYD1, FXYD3 and FXYD5 is elevated in the lungs of Acute respiratory distress syndrome patients
Title: FXYD1 negatively regulates Na(+)/K(+)-ATPase activity in lung alveolar epithelial cells.

Dysadherin-silenced side population cells exhibited reduced expression of Bcl-2 and Bax compared with that prior to silencing.
Title: Aberrantly regulated dysadherin and B-cell lymphoma 2/B-cell lymphoma 2-associated X enhances tumorigenesis and DNA targeting drug resistance of liver cancer stem cells.

These results suggest a role of let-7a down-regulation in the development of thyroid neoplasias of the follicular histotype, likely regulating dysadherin protein expression levels.
Title: Let-7a down-regulation plays a role in thyroid neoplasias of follicular histotype affecting cell adhesion and migration through its ability to target the FXYD5 (Dysadherin) gene.

The following FXYD5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FXYD5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu52314 NM_001320912.2
Latest version!		 Homo sapiens FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu01680 NM_001164605.2
Latest version!		 Homo sapiens FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu01680 NM_144779.3
Latest version!		 Homo sapiens FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu01680 NM_014164.6
Latest version!		 Homo sapiens FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu52315 NM_001320913.2
Latest version!		 Homo sapiens FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
View Old Accession Versions >>
OHu01680 NM_014164.5 Homo sapiens FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu52314 NM_001320912.1 Homo sapiens FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu52315 NM_001320913.1 Homo sapiens FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu01680 NM_001164605.1 Homo sapiens FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu01680 NM_144779.2 Homo sapiens FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu52314
Clone ID Related Accession (Same CDS sequence) NM_001320912.1 , NM_001320912.2
Accession Version NM_001320912.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 585bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-06-30
Organism Homo sapiens(human)
Product FXYD domain-containing ion transport regulator 5 isoform 2 precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG025158.1, BM550889.1, AC002390.1 and AI424071.1. On Mar 10, 2016 this sequence version replaced XM_006723245.2. Transcript Variant: This variant (5) differs in the 5' and 3' UTRs and uses an alternate splice site in the penultimate exon, compared to variant 1. It encodes isoform 2, which is longer and has a distinct C-tetminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM550889.1, BM810965.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2155590, SAMEA2162823 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
ATGTCGCCCT CTGGTCGCCT GTGTCTTCTC ACCATCGTTG GCCTGATTCT CCCCACCAGA 
GGACAGACGT TGAAAGATAC CACGTCCAGT TCTTCAGCAG ACTCAACTAT CATGGACATT 
CAGGTCCCGA CACGAGCCCC AGATGCAGTC TACACAGAAC TCCAGCCCAC CTCTCCAACC 
CCAACCTGGC CTGCTGATGA AACACCACAA CCCCAGACCC AGACCCAGCA ACTGGAAGGA 
ACGGATGGGC CTCTAGTGAC AGATCCAGAG ACACACAAGA GCACCAAAGC AGCTCATCCC 
ACTGATGACA CCACGACGCT CTCTGAGAGA CCATCCCCAA GCACAGACGT CCAGACAGAC 
CCCCAGACCC TCAAGCCATC TGGTTTTCAT GAGGATGACC CCTTCTTCTA TGATGAACAC 
ACCCTCCGGA AACGGGGGCT GTTGGTCGCA GCTGTGCTGT TCATCACAGG CATCATCATC 
CTCACCAGTG AGAACTGGGG TTGGGTGGGA AGGACACCAA GACCAGGAGG GGGACTTATG 
ACGGAGGGCT GGGTTCTCAA AGGTCCCTGG GCAATTTTGT TTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001307841.1
CDS89..673
Translation

Target ORF information:

RefSeq Version NM_001320912.1
Organism Homo sapiens(human)
Definition Homo sapiens FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001320912.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGTCGCCCT CTGGTCGCCT GTGTCTTCTC ACCATCGTTG GCCTGATTCT CCCCACCAGA 
GGACAGACGT TGAAAGATAC CACGTCCAGT TCTTCAGCAG ACTCAACTAT CATGGACATT 
CAGGTCCCGA CACGAGCCCC AGATGCAGTC TACACAGAAC TCCAGCCCAC CTCTCCAACC 
CCAACCTGGC CTGCTGATGA AACACCACAA CCCCAGACCC AGACCCAGCA ACTGGAAGGA 
ACGGATGGGC CTCTAGTGAC AGATCCAGAG ACACACAAGA GCACCAAAGC AGCTCATCCC 
ACTGATGACA CCACGACGCT CTCTGAGAGA CCATCCCCAA GCACAGACGT CCAGACAGAC 
CCCCAGACCC TCAAGCCATC TGGTTTTCAT GAGGATGACC CCTTCTTCTA TGATGAACAC 
ACCCTCCGGA AACGGGGGCT GTTGGTCGCA GCTGTGCTGT TCATCACAGG CATCATCATC 
CTCACCAGTG AGAACTGGGG TTGGGTGGGA AGGACACCAA GACCAGGAGG GGGACTTATG 
ACGGAGGGCT GGGTTCTCAA AGGTCCCTGG GCAATTTTGT TTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu52314
Clone ID Related Accession (Same CDS sequence) NM_001320912.1 , NM_001320912.2
Accession Version NM_001320912.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 585bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-20
Organism Homo sapiens(human)
Product FXYD domain-containing ion transport regulator 5 isoform 2 precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG025158.1, BM550889.1, AC002390.1 and AI424071.1. On May 31, 2019 this sequence version replaced NM_001320912.1. Transcript Variant: This variant (5) differs in the 5' and 3' UTRs and uses an alternate splice site in the penultimate exon, compared to variant 1. It encodes isoform 2, which is longer and has a distinct C-tetminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM550889.1, BM810965.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2155590, SAMEA2162823 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
ATGTCGCCCT CTGGTCGCCT GTGTCTTCTC ACCATCGTTG GCCTGATTCT CCCCACCAGA 
GGACAGACGT TGAAAGATAC CACGTCCAGT TCTTCAGCAG ACTCAACTAT CATGGACATT 
CAGGTCCCGA CACGAGCCCC AGATGCAGTC TACACAGAAC TCCAGCCCAC CTCTCCAACC 
CCAACCTGGC CTGCTGATGA AACACCACAA CCCCAGACCC AGACCCAGCA ACTGGAAGGA 
ACGGATGGGC CTCTAGTGAC AGATCCAGAG ACACACAAGA GCACCAAAGC AGCTCATCCC 
ACTGATGACA CCACGACGCT CTCTGAGAGA CCATCCCCAA GCACAGACGT CCAGACAGAC 
CCCCAGACCC TCAAGCCATC TGGTTTTCAT GAGGATGACC CCTTCTTCTA TGATGAACAC 
ACCCTCCGGA AACGGGGGCT GTTGGTCGCA GCTGTGCTGT TCATCACAGG CATCATCATC 
CTCACCAGTG AGAACTGGGG TTGGGTGGGA AGGACACCAA GACCAGGAGG GGGACTTATG 
ACGGAGGGCT GGGTTCTCAA AGGTCCCTGG GCAATTTTGT TTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001307841.1
CDS76..660
Translation

Target ORF information:

RefSeq Version NM_001320912.2
Organism Homo sapiens(human)
Definition Homo sapiens FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001320912.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGTCGCCCT CTGGTCGCCT GTGTCTTCTC ACCATCGTTG GCCTGATTCT CCCCACCAGA 
GGACAGACGT TGAAAGATAC CACGTCCAGT TCTTCAGCAG ACTCAACTAT CATGGACATT 
CAGGTCCCGA CACGAGCCCC AGATGCAGTC TACACAGAAC TCCAGCCCAC CTCTCCAACC 
CCAACCTGGC CTGCTGATGA AACACCACAA CCCCAGACCC AGACCCAGCA ACTGGAAGGA 
ACGGATGGGC CTCTAGTGAC AGATCCAGAG ACACACAAGA GCACCAAAGC AGCTCATCCC 
ACTGATGACA CCACGACGCT CTCTGAGAGA CCATCCCCAA GCACAGACGT CCAGACAGAC 
CCCCAGACCC TCAAGCCATC TGGTTTTCAT GAGGATGACC CCTTCTTCTA TGATGAACAC 
ACCCTCCGGA AACGGGGGCT GTTGGTCGCA GCTGTGCTGT TCATCACAGG CATCATCATC 
CTCACCAGTG AGAACTGGGG TTGGGTGGGA AGGACACCAA GACCAGGAGG GGGACTTATG 
ACGGAGGGCT GGGTTCTCAA AGGTCCCTGG GCAATTTTGT TTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu01680
Clone ID Related Accession (Same CDS sequence) NM_014164.5 , NM_014164.6 , NM_001164605.1 , NM_144779.2 , NM_001164605.2 , NM_144779.3
Accession Version NM_014164.5 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 537bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-06-23
Organism Homo sapiens(human)
Product FXYD domain-containing ion transport regulator 5 isoform 1 precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Kathleen Sweadner. The reference sequence was derived from BM839241.1, AB072911.1, BQ941279.1 and AI424071.1. This sequence is a reference standard in the RefSeqGene project. On Sep 3, 2009 this sequence version replaced NM_014164.4. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279839.464.1, SRR1163655.610293.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
ATGTCGCCCT CTGGTCGCCT GTGTCTTCTC ACCATCGTTG GCCTGATTCT CCCCACCAGA 
GGACAGACGT TGAAAGATAC CACGTCCAGT TCTTCAGCAG ACTCAACTAT CATGGACATT 
CAGGTCCCGA CACGAGCCCC AGATGCAGTC TACACAGAAC TCCAGCCCAC CTCTCCAACC 
CCAACCTGGC CTGCTGATGA AACACCACAA CCCCAGACCC AGACCCAGCA ACTGGAAGGA 
ACGGATGGGC CTCTAGTGAC AGATCCAGAG ACACACAAGA GCACCAAAGC AGCTCATCCC 
ACTGATGACA CCACGACGCT CTCTGAGAGA CCATCCCCAA GCACAGACGT CCAGACAGAC 
CCCCAGACCC TCAAGCCATC TGGTTTTCAT GAGGATGACC CCTTCTTCTA TGATGAACAC 
ACCCTCCGGA AACGGGGGCT GTTGGTCGCA GCTGTGCTGT TCATCACAGG CATCATCATC 
CTCACCAGTG GCAAGTGCAG GCAGCTGTCC CGGTTATGCC GGAATCGTTG CAGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_054883.3
CDS89..625
Translation

Target ORF information:

RefSeq Version NM_014164.5
Organism Homo sapiens(human)
Definition Homo sapiens FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_014164.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGTCGCCCT CTGGTCGCCT GTGTCTTCTC ACCATCGTTG GCCTGATTCT CCCCACCAGA 
GGACAGACGT TGAAAGATAC CACGTCCAGT TCTTCAGCAG ACTCAACTAT CATGGACATT 
CAGGTCCCGA CACGAGCCCC AGATGCAGTC TACACAGAAC TCCAGCCCAC CTCTCCAACC 
CCAACCTGGC CTGCTGATGA AACACCACAA CCCCAGACCC AGACCCAGCA ACTGGAAGGA 
ACGGATGGGC CTCTAGTGAC AGATCCAGAG ACACACAAGA GCACCAAAGC AGCTCATCCC 
ACTGATGACA CCACGACGCT CTCTGAGAGA CCATCCCCAA GCACAGACGT CCAGACAGAC 
CCCCAGACCC TCAAGCCATC TGGTTTTCAT GAGGATGACC CCTTCTTCTA TGATGAACAC 
ACCCTCCGGA AACGGGGGCT GTTGGTCGCA GCTGTGCTGT TCATCACAGG CATCATCATC 
CTCACCAGTG GCAAGTGCAG GCAGCTGTCC CGGTTATGCC GGAATCGTTG CAGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu01680
Clone ID Related Accession (Same CDS sequence) NM_014164.5 , NM_014164.6 , NM_001164605.1 , NM_144779.2 , NM_001164605.2 , NM_144779.3
Accession Version NM_014164.6 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 537bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-31
Organism Homo sapiens(human)
Product FXYD domain-containing ion transport regulator 5 isoform 1 precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Kathleen Sweadner. The reference sequence was derived from BM839241.1, AB072911.1, BQ941279.1 and AI424071.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_014164.5. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.652985.1, SRR1163655.364398.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000392219.7/ ENSP00000376053.2 RefSeq Select criteria :: based on expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
ATGTCGCCCT CTGGTCGCCT GTGTCTTCTC ACCATCGTTG GCCTGATTCT CCCCACCAGA 
GGACAGACGT TGAAAGATAC CACGTCCAGT TCTTCAGCAG ACTCAACTAT CATGGACATT 
CAGGTCCCGA CACGAGCCCC AGATGCAGTC TACACAGAAC TCCAGCCCAC CTCTCCAACC 
CCAACCTGGC CTGCTGATGA AACACCACAA CCCCAGACCC AGACCCAGCA ACTGGAAGGA 
ACGGATGGGC CTCTAGTGAC AGATCCAGAG ACACACAAGA GCACCAAAGC AGCTCATCCC 
ACTGATGACA CCACGACGCT CTCTGAGAGA CCATCCCCAA GCACAGACGT CCAGACAGAC 
CCCCAGACCC TCAAGCCATC TGGTTTTCAT GAGGATGACC CCTTCTTCTA TGATGAACAC 
ACCCTCCGGA AACGGGGGCT GTTGGTCGCA GCTGTGCTGT TCATCACAGG CATCATCATC 
CTCACCAGTG GCAAGTGCAG GCAGCTGTCC CGGTTATGCC GGAATCGTTG CAGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_054883.3
CDS76..612
Translation

Target ORF information:

RefSeq Version NM_014164.6
Organism Homo sapiens(human)
Definition Homo sapiens FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_014164.6

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGTCGCCCT CTGGTCGCCT GTGTCTTCTC ACCATCGTTG GCCTGATTCT CCCCACCAGA 
GGACAGACGT TGAAAGATAC CACGTCCAGT TCTTCAGCAG ACTCAACTAT CATGGACATT 
CAGGTCCCGA CACGAGCCCC AGATGCAGTC TACACAGAAC TCCAGCCCAC CTCTCCAACC 
CCAACCTGGC CTGCTGATGA AACACCACAA CCCCAGACCC AGACCCAGCA ACTGGAAGGA 
ACGGATGGGC CTCTAGTGAC AGATCCAGAG ACACACAAGA GCACCAAAGC AGCTCATCCC 
ACTGATGACA CCACGACGCT CTCTGAGAGA CCATCCCCAA GCACAGACGT CCAGACAGAC 
CCCCAGACCC TCAAGCCATC TGGTTTTCAT GAGGATGACC CCTTCTTCTA TGATGAACAC 
ACCCTCCGGA AACGGGGGCT GTTGGTCGCA GCTGTGCTGT TCATCACAGG CATCATCATC 
CTCACCAGTG GCAAGTGCAG GCAGCTGTCC CGGTTATGCC GGAATCGTTG CAGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu01680
Clone ID Related Accession (Same CDS sequence) NM_014164.5 , NM_014164.6 , NM_001164605.1 , NM_144779.2 , NM_001164605.2 , NM_144779.3
Accession Version NM_001164605.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 537bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-06-23
Organism Homo sapiens(human)
Product FXYD domain-containing ion transport regulator 5 isoform 1 precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU164524.1 and AI424071.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU164524.1, BQ051926.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
ATGTCGCCCT CTGGTCGCCT GTGTCTTCTC ACCATCGTTG GCCTGATTCT CCCCACCAGA 
GGACAGACGT TGAAAGATAC CACGTCCAGT TCTTCAGCAG ACTCAACTAT CATGGACATT 
CAGGTCCCGA CACGAGCCCC AGATGCAGTC TACACAGAAC TCCAGCCCAC CTCTCCAACC 
CCAACCTGGC CTGCTGATGA AACACCACAA CCCCAGACCC AGACCCAGCA ACTGGAAGGA 
ACGGATGGGC CTCTAGTGAC AGATCCAGAG ACACACAAGA GCACCAAAGC AGCTCATCCC 
ACTGATGACA CCACGACGCT CTCTGAGAGA CCATCCCCAA GCACAGACGT CCAGACAGAC 
CCCCAGACCC TCAAGCCATC TGGTTTTCAT GAGGATGACC CCTTCTTCTA TGATGAACAC 
ACCCTCCGGA AACGGGGGCT GTTGGTCGCA GCTGTGCTGT TCATCACAGG CATCATCATC 
CTCACCAGTG GCAAGTGCAG GCAGCTGTCC CGGTTATGCC GGAATCGTTG CAGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001158077.1
CDS92..628
Translation

Target ORF information:

RefSeq Version NM_001164605.1
Organism Homo sapiens(human)
Definition Homo sapiens FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001164605.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGTCGCCCT CTGGTCGCCT GTGTCTTCTC ACCATCGTTG GCCTGATTCT CCCCACCAGA 
GGACAGACGT TGAAAGATAC CACGTCCAGT TCTTCAGCAG ACTCAACTAT CATGGACATT 
CAGGTCCCGA CACGAGCCCC AGATGCAGTC TACACAGAAC TCCAGCCCAC CTCTCCAACC 
CCAACCTGGC CTGCTGATGA AACACCACAA CCCCAGACCC AGACCCAGCA ACTGGAAGGA 
ACGGATGGGC CTCTAGTGAC AGATCCAGAG ACACACAAGA GCACCAAAGC AGCTCATCCC 
ACTGATGACA CCACGACGCT CTCTGAGAGA CCATCCCCAA GCACAGACGT CCAGACAGAC 
CCCCAGACCC TCAAGCCATC TGGTTTTCAT GAGGATGACC CCTTCTTCTA TGATGAACAC 
ACCCTCCGGA AACGGGGGCT GTTGGTCGCA GCTGTGCTGT TCATCACAGG CATCATCATC 
CTCACCAGTG GCAAGTGCAG GCAGCTGTCC CGGTTATGCC GGAATCGTTG CAGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu01680
Clone ID Related Accession (Same CDS sequence) NM_014164.5 , NM_014164.6 , NM_001164605.1 , NM_144779.2 , NM_001164605.2 , NM_144779.3
Accession Version NM_144779.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 537bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-06-23
Organism Homo sapiens(human)
Product FXYD domain-containing ion transport regulator 5 isoform 1 precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Kathleen Sweadner. The reference sequence was derived from BQ440098.1, AC020907.6 and AI424071.1. On Sep 3, 2009 this sequence version replaced NM_144779.1. Transcript Variant: This variant (1) encodes the same protein as variants 2 and 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BQ440098.1, SRR1163658.289325.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
ATGTCGCCCT CTGGTCGCCT GTGTCTTCTC ACCATCGTTG GCCTGATTCT CCCCACCAGA 
GGACAGACGT TGAAAGATAC CACGTCCAGT TCTTCAGCAG ACTCAACTAT CATGGACATT 
CAGGTCCCGA CACGAGCCCC AGATGCAGTC TACACAGAAC TCCAGCCCAC CTCTCCAACC 
CCAACCTGGC CTGCTGATGA AACACCACAA CCCCAGACCC AGACCCAGCA ACTGGAAGGA 
ACGGATGGGC CTCTAGTGAC AGATCCAGAG ACACACAAGA GCACCAAAGC AGCTCATCCC 
ACTGATGACA CCACGACGCT CTCTGAGAGA CCATCCCCAA GCACAGACGT CCAGACAGAC 
CCCCAGACCC TCAAGCCATC TGGTTTTCAT GAGGATGACC CCTTCTTCTA TGATGAACAC 
ACCCTCCGGA AACGGGGGCT GTTGGTCGCA GCTGTGCTGT TCATCACAGG CATCATCATC 
CTCACCAGTG GCAAGTGCAG GCAGCTGTCC CGGTTATGCC GGAATCGTTG CAGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_659003.1
CDS102..638
Translation

Target ORF information:

RefSeq Version NM_144779.2
Organism Homo sapiens(human)
Definition Homo sapiens FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_144779.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGTCGCCCT CTGGTCGCCT GTGTCTTCTC ACCATCGTTG GCCTGATTCT CCCCACCAGA 
GGACAGACGT TGAAAGATAC CACGTCCAGT TCTTCAGCAG ACTCAACTAT CATGGACATT 
CAGGTCCCGA CACGAGCCCC AGATGCAGTC TACACAGAAC TCCAGCCCAC CTCTCCAACC 
CCAACCTGGC CTGCTGATGA AACACCACAA CCCCAGACCC AGACCCAGCA ACTGGAAGGA 
ACGGATGGGC CTCTAGTGAC AGATCCAGAG ACACACAAGA GCACCAAAGC AGCTCATCCC 
ACTGATGACA CCACGACGCT CTCTGAGAGA CCATCCCCAA GCACAGACGT CCAGACAGAC 
CCCCAGACCC TCAAGCCATC TGGTTTTCAT GAGGATGACC CCTTCTTCTA TGATGAACAC 
ACCCTCCGGA AACGGGGGCT GTTGGTCGCA GCTGTGCTGT TCATCACAGG CATCATCATC 
CTCACCAGTG GCAAGTGCAG GCAGCTGTCC CGGTTATGCC GGAATCGTTG CAGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu01680
Clone ID Related Accession (Same CDS sequence) NM_014164.5 , NM_014164.6 , NM_001164605.1 , NM_144779.2 , NM_001164605.2 , NM_144779.3
Accession Version NM_001164605.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 537bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2020-01-03
Organism Homo sapiens(human)
Product FXYD domain-containing ion transport regulator 5 isoform 1 precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020907.6, BU164524.1 and AI424071.1. On May 31, 2019 this sequence version replaced NM_001164605.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU164524.1, BQ051926.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
ATGTCGCCCT CTGGTCGCCT GTGTCTTCTC ACCATCGTTG GCCTGATTCT CCCCACCAGA 
GGACAGACGT TGAAAGATAC CACGTCCAGT TCTTCAGCAG ACTCAACTAT CATGGACATT 
CAGGTCCCGA CACGAGCCCC AGATGCAGTC TACACAGAAC TCCAGCCCAC CTCTCCAACC 
CCAACCTGGC CTGCTGATGA AACACCACAA CCCCAGACCC AGACCCAGCA ACTGGAAGGA 
ACGGATGGGC CTCTAGTGAC AGATCCAGAG ACACACAAGA GCACCAAAGC AGCTCATCCC 
ACTGATGACA CCACGACGCT CTCTGAGAGA CCATCCCCAA GCACAGACGT CCAGACAGAC 
CCCCAGACCC TCAAGCCATC TGGTTTTCAT GAGGATGACC CCTTCTTCTA TGATGAACAC 
ACCCTCCGGA AACGGGGGCT GTTGGTCGCA GCTGTGCTGT TCATCACAGG CATCATCATC 
CTCACCAGTG GCAAGTGCAG GCAGCTGTCC CGGTTATGCC GGAATCGTTG CAGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001158077.1
CDS299..835
Translation

Target ORF information:

RefSeq Version NM_001164605.2
Organism Homo sapiens(human)
Definition Homo sapiens FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001164605.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGTCGCCCT CTGGTCGCCT GTGTCTTCTC ACCATCGTTG GCCTGATTCT CCCCACCAGA 
GGACAGACGT TGAAAGATAC CACGTCCAGT TCTTCAGCAG ACTCAACTAT CATGGACATT 
CAGGTCCCGA CACGAGCCCC AGATGCAGTC TACACAGAAC TCCAGCCCAC CTCTCCAACC 
CCAACCTGGC CTGCTGATGA AACACCACAA CCCCAGACCC AGACCCAGCA ACTGGAAGGA 
ACGGATGGGC CTCTAGTGAC AGATCCAGAG ACACACAAGA GCACCAAAGC AGCTCATCCC 
ACTGATGACA CCACGACGCT CTCTGAGAGA CCATCCCCAA GCACAGACGT CCAGACAGAC 
CCCCAGACCC TCAAGCCATC TGGTTTTCAT GAGGATGACC CCTTCTTCTA TGATGAACAC 
ACCCTCCGGA AACGGGGGCT GTTGGTCGCA GCTGTGCTGT TCATCACAGG CATCATCATC 
CTCACCAGTG GCAAGTGCAG GCAGCTGTCC CGGTTATGCC GGAATCGTTG CAGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu01680
Clone ID Related Accession (Same CDS sequence) NM_014164.5 , NM_014164.6 , NM_001164605.1 , NM_144779.2 , NM_001164605.2 , NM_144779.3
Accession Version NM_144779.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 537bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2020-01-03
Organism Homo sapiens(human)
Product FXYD domain-containing ion transport regulator 5 isoform 1 precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Kathleen Sweadner. The reference sequence was derived from AC020907.6, BQ440098.1 and AI424071.1. On May 31, 2019 this sequence version replaced NM_144779.2. Transcript Variant: This variant (1) encodes the same protein as variants 2 and 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.157292.1, SRR7346977.33535.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
ATGTCGCCCT CTGGTCGCCT GTGTCTTCTC ACCATCGTTG GCCTGATTCT CCCCACCAGA 
GGACAGACGT TGAAAGATAC CACGTCCAGT TCTTCAGCAG ACTCAACTAT CATGGACATT 
CAGGTCCCGA CACGAGCCCC AGATGCAGTC TACACAGAAC TCCAGCCCAC CTCTCCAACC 
CCAACCTGGC CTGCTGATGA AACACCACAA CCCCAGACCC AGACCCAGCA ACTGGAAGGA 
ACGGATGGGC CTCTAGTGAC AGATCCAGAG ACACACAAGA GCACCAAAGC AGCTCATCCC 
ACTGATGACA CCACGACGCT CTCTGAGAGA CCATCCCCAA GCACAGACGT CCAGACAGAC 
CCCCAGACCC TCAAGCCATC TGGTTTTCAT GAGGATGACC CCTTCTTCTA TGATGAACAC 
ACCCTCCGGA AACGGGGGCT GTTGGTCGCA GCTGTGCTGT TCATCACAGG CATCATCATC 
CTCACCAGTG GCAAGTGCAG GCAGCTGTCC CGGTTATGCC GGAATCGTTG CAGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_659003.1
CDS136..672
Translation

Target ORF information:

RefSeq Version NM_144779.3
Organism Homo sapiens(human)
Definition Homo sapiens FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_144779.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGTCGCCCT CTGGTCGCCT GTGTCTTCTC ACCATCGTTG GCCTGATTCT CCCCACCAGA 
GGACAGACGT TGAAAGATAC CACGTCCAGT TCTTCAGCAG ACTCAACTAT CATGGACATT 
CAGGTCCCGA CACGAGCCCC AGATGCAGTC TACACAGAAC TCCAGCCCAC CTCTCCAACC 
CCAACCTGGC CTGCTGATGA AACACCACAA CCCCAGACCC AGACCCAGCA ACTGGAAGGA 
ACGGATGGGC CTCTAGTGAC AGATCCAGAG ACACACAAGA GCACCAAAGC AGCTCATCCC 
ACTGATGACA CCACGACGCT CTCTGAGAGA CCATCCCCAA GCACAGACGT CCAGACAGAC 
CCCCAGACCC TCAAGCCATC TGGTTTTCAT GAGGATGACC CCTTCTTCTA TGATGAACAC 
ACCCTCCGGA AACGGGGGCT GTTGGTCGCA GCTGTGCTGT TCATCACAGG CATCATCATC 
CTCACCAGTG GCAAGTGCAG GCAGCTGTCC CGGTTATGCC GGAATCGTTG CAGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu52315
Clone ID Related Accession (Same CDS sequence) NM_001320913.1 , NM_001320913.2
Accession Version NM_001320913.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 444bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-12-04
Organism Homo sapiens(human)
Product FXYD domain-containing ion transport regulator 5 isoform 3 precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM839241.1, AB072911.1, BQ941279.1 and AI424071.1. On Mar 10, 2016 this sequence version replaced XM_006723247.2. Transcript Variant: This variant (6) differs in the 5' UTR and lacks an alternate in-frame exon in the coding region, compared to variant 1. It encodes isoform 3, which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.520240.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
ATGTCGCCCT CTGGTCGCCT GTGTCTTCTC ACCATCGTTG GCCTGATTCT CCCCACCAGA 
GGACAGACGT TGAAAGATAC CACGTCCAGT TCTTCAGCAG ACTCAACTAT CATGGACATT 
CAGGTCCCGA CACGAGCCCC AGATGCAGTC TACACAGAAC TCCAGCCCAC CTCTCCAACC 
CCAACCTGGC CTGCTGATGC TCATCCCACT GATGACACCA CGACGCTCTC TGAGAGACCA 
TCCCCAAGCA CAGACGTCCA GACAGACCCC CAGACCCTCA AGCCATCTGG TTTTCATGAG 
GATGACCCCT TCTTCTATGA TGAACACACC CTCCGGAAAC GGGGGCTGTT GGTCGCAGCT 
GTGCTGTTCA TCACAGGCAT CATCATCCTC ACCAGTGGCA AGTGCAGGCA GCTGTCCCGG 
TTATGCCGGA ATCGTTGCAG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001307842.1
CDS89..532
Translation

Target ORF information:

RefSeq Version NM_001320913.1
Organism Homo sapiens(human)
Definition Homo sapiens FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001320913.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGTCGCCCT CTGGTCGCCT GTGTCTTCTC ACCATCGTTG GCCTGATTCT CCCCACCAGA 
GGACAGACGT TGAAAGATAC CACGTCCAGT TCTTCAGCAG ACTCAACTAT CATGGACATT 
CAGGTCCCGA CACGAGCCCC AGATGCAGTC TACACAGAAC TCCAGCCCAC CTCTCCAACC 
CCAACCTGGC CTGCTGATGC TCATCCCACT GATGACACCA CGACGCTCTC TGAGAGACCA 
TCCCCAAGCA CAGACGTCCA GACAGACCCC CAGACCCTCA AGCCATCTGG TTTTCATGAG 
GATGACCCCT TCTTCTATGA TGAACACACC CTCCGGAAAC GGGGGCTGTT GGTCGCAGCT 
GTGCTGTTCA TCACAGGCAT CATCATCCTC ACCAGTGGCA AGTGCAGGCA GCTGTCCCGG 
TTATGCCGGA ATCGTTGCAG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu52315
Clone ID Related Accession (Same CDS sequence) NM_001320913.1 , NM_001320913.2
Accession Version NM_001320913.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 444bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-20
Organism Homo sapiens(human)
Product FXYD domain-containing ion transport regulator 5 isoform 3 precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM839241.1, AB072911.1, BQ941279.1 and AI424071.1. On May 31, 2019 this sequence version replaced NM_001320913.1. Transcript Variant: This variant (6) differs in the 5' UTR and lacks an alternate in-frame exon in the coding region, compared to variant 1. It encodes isoform 3, which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.520240.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
ATGTCGCCCT CTGGTCGCCT GTGTCTTCTC ACCATCGTTG GCCTGATTCT CCCCACCAGA 
GGACAGACGT TGAAAGATAC CACGTCCAGT TCTTCAGCAG ACTCAACTAT CATGGACATT 
CAGGTCCCGA CACGAGCCCC AGATGCAGTC TACACAGAAC TCCAGCCCAC CTCTCCAACC 
CCAACCTGGC CTGCTGATGC TCATCCCACT GATGACACCA CGACGCTCTC TGAGAGACCA 
TCCCCAAGCA CAGACGTCCA GACAGACCCC CAGACCCTCA AGCCATCTGG TTTTCATGAG 
GATGACCCCT TCTTCTATGA TGAACACACC CTCCGGAAAC GGGGGCTGTT GGTCGCAGCT 
GTGCTGTTCA TCACAGGCAT CATCATCCTC ACCAGTGGCA AGTGCAGGCA GCTGTCCCGG 
TTATGCCGGA ATCGTTGCAG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001307842.1
CDS76..519
Translation

Target ORF information:

RefSeq Version NM_001320913.2
Organism Homo sapiens(human)
Definition Homo sapiens FXYD domain containing ion transport regulator 5 (FXYD5), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001320913.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGTCGCCCT CTGGTCGCCT GTGTCTTCTC ACCATCGTTG GCCTGATTCT CCCCACCAGA 
GGACAGACGT TGAAAGATAC CACGTCCAGT TCTTCAGCAG ACTCAACTAT CATGGACATT 
CAGGTCCCGA CACGAGCCCC AGATGCAGTC TACACAGAAC TCCAGCCCAC CTCTCCAACC 
CCAACCTGGC CTGCTGATGC TCATCCCACT GATGACACCA CGACGCTCTC TGAGAGACCA 
TCCCCAAGCA CAGACGTCCA GACAGACCCC CAGACCCTCA AGCCATCTGG TTTTCATGAG 
GATGACCCCT TCTTCTATGA TGAACACACC CTCCGGAAAC GGGGGCTGTT GGTCGCAGCT 
GTGCTGTTCA TCACAGGCAT CATCATCCTC ACCAGTGGCA AGTGCAGGCA GCTGTCCCGG 
TTATGCCGGA ATCGTTGCAG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

The O-glycosylated ectodomain of FXYD5 impairs adhesion by disrupting cell-cell trans-dimerization of Na,K-ATPase ?1 subunits.
Journal of cell science129(12)2394-406(2016 06)
Tokhtaeva E,Sun H,Deiss-Yehiely N,Wen Y,Soni PN,Gabrielli NM,Marcus EA,Ridge KM,Sachs G,Vazquez-Levin M,Sznajder JI,Vagin O,Dada LA


book

FXYD5 Protein Has a Pro-inflammatory Role in Epithelial Cells.
The Journal of biological chemistry291(21)11072-82(2016 May)
Lubarski-Gotliv I,Asher C,Dada LA,Garty H


book

FXYD1 negatively regulates Na(+)/K(+)-ATPase activity in lung alveolar epithelial cells.
Respiratory physiology & neurobiology22054-61(2016 Jan)
Wujak ?A,Blume A,Balo?lu E,Wygrecka M,Wygowski J,Herold S,Mayer K,Vad?sz I,Besuch P,Mairb?url H,Seeger W,Morty RE


book

Aberrantly regulated dysadherin and B-cell lymphoma 2/B-cell lymphoma 2-associated X enhances tumorigenesis and DNA targeting drug resistance of liver cancer stem cells.
Molecular medicine reports12(5)7239-46(2015 Nov)
Jiang N,Chen W,Zhang JW,Li Y,Zeng XC,Zhang T,Fu BS,Yi HM,Zhang Q


book

Modulation of cell polarization by the Na+-K+-ATPase-associated protein FXYD5 (dysadherin).
American journal of physiology. Cell physiology306(11)C1080-8(2014 Jun)
Lubarski I,Asher C,Garty H