×

UCHL3 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol UCHL3
Entrez Gene ID 7347
Full Name ubiquitin C-terminal hydrolase L3
Synonyms UCH-L3
General protein information
Preferred Names

ubiquitin C-terminal hydrolase L3

Names

ubiquitin carboxyl-terminal hydrolase isozyme L3
testicular tissue protein Li 221
ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
ubiquitin thioesterase L3
ubiquitin thiolesterase

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

13

13q22.2

Summary The protein encoded by this gene is a member of the deubiquitinating enzyme family. Members of this family are proteases that catalyze the removal of ubiquitin from polypeptides and are divided into five classes, depending on the mechanism of catalysis. This protein may hydrolyze the ubiquitinyl-N-epsilon amide bond of ubiquitinated proteins to regenerate ubiquitin for another catalytic cycle. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012].
Disorder MIM:

603090


mRNA and Protein(s)

mRNA Protein Name
NM_006002.4 NP_005993.1 ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform 2
XM_011535214.2 XP_011533516.1 ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform X4
XM_017020726.1 XP_016876215.1 ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform X5
XM_017020725.1 XP_016876214.1 ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform X3
XM_017020727.1 XP_016876216.1 ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform X6
XM_011535213.2 XP_011533515.1 ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform X2
XM_011535212.1 XP_011533514.1 ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform X1
NM_001270952.1 NP_001257881.1 ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform 1



Gallus gallus (chicken) UCHL3 NP_990156.1
Drosophila melanogaster (fruit fly) Uch NP_001188681.1
Saccharomyces cerevisiae (baker's yeast) YUH1 NP_012633.1
Canis lupus familiaris (dog) UCHL3 XP_534147.3
Pan troglodytes (chimpanzee) UCHL3 XP_003314228.1
Bos taurus (cattle) UCHL3 NP_001035631.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP012113 XP_320415.2
Kluyveromyces lactis KLLA0E09967g XP_454399.1
Eremothecium gossypii AGOS_AGL314C NP_986353.1
Oryza sativa (rice) Os02g0654500 NP_001047612.1
Homo sapiens (human) UCHL3 NP_005993.1
Neurospora crassa NCU06372 XP_962406.1
Mus musculus (house mouse) Uchl3 NP_057932.2
Rattus norvegicus (Norway rat) Uchl3 NP_001103635.1
Danio rerio (zebrafish) uchl3 NP_001019576.1
Magnaporthe oryzae (rice blast fungus) MGG_01683 XP_003714672.1
Arabidopsis thaliana (thale cress) UCH3 NP_193484.1
Xenopus tropicalis (tropical clawed frog) uchl3 NP_001017120.1
Macaca mulatta (rhesus monkey) UCHL3 XP_001085879.2


Related articles in PubMed

Characterizing substrate selectivity of ubiquitin C-terminal hydrolase-L3 using engineered α-linked ubiquitin substrates.
Navarro MF, Carmody L, Romo-Fewell O, Lokensgard ME, Love JJ
Biochemistry53(51)8031-42(2014 Dec)

Ubiquitin C-terminal hydrolase-L3 regulates EMT process and cancer metastasis in prostate cell lines.
Song HM, Lee JE, Kim JH
Biochemical and biophysical research communications452(3)722-7(2014 Sep)

Genome-wide association study in a Chinese population with diabetic retinopathy.
Sheu WH, Kuo JZ, Lee IT, Hung YJ, Lee WJ, Tsai HY, Wang JS, Goodarzi MO, Klein R, Klein BE, Ipp E, Lin SY, Guo X, Hsieh CH, Taylor KD, Fu CP, Rotter JI, Chen YD
Human molecular genetics22(15)3165-73(2013 Aug)

A novel RNAi lethality rescue screen to identify regulators of adipogenesis.
van Beekum O, Gao Y, Berger R, Koppen A, Kalkhoven E
PloS one7(6)e37680(2012)

Structure of the ubiquitin hydrolase UCH-L3 complexed with a suicide substrate.
Misaghi S, Galardy PJ, Meester WJ, Ovaa H, Ploegh HL, Gaudet R
The Journal of biological chemistry280(2)1512-20(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study demonstrated positive correlations between the level and activity of UCHL3 and sperm characteristics and function suggesting that UCHL3 may play a role in male infertility.
Title: Ubiquitin Carboxy-Terminal HydrolaseL3 Correlates with Human Sperm Count, Motility and Fertilization.

Using a series of engineered protein substrates, which are similar in size yet differ in secondary structure, we demonstrate that thermal stability is a key factor that significantly affects UCH-L3 hydrolysis.
Title: Characterizing substrate selectivity of ubiquitin C-terminal hydrolase-L3 using engineered α-linked ubiquitin substrates.

UCH-L3 is a novel regulator of epithelial-mesenchymal transition and cell migration in prostate cancer cells.
Title: Ubiquitin C-terminal hydrolase-L3 regulates EMT process and cancer metastasis in prostate cell lines.

Data identified of UCHL3 as an essential deubiquitinase in adipogenesis.
Title: A novel RNAi lethality rescue screen to identify regulators of adipogenesis.

impaired UCH-L3 function may contribute to the accumulation of full length UBB(+1) in various pathologie
Title: Mutant ubiquitin (UBB+1) associated with neurodegenerative disorders is hydrolyzed by ubiquitin C-terminal hydrolase L3 (UCH-L3).

The following UCHL3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UCHL3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

Free DYK Tag Antibody

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu10261
NM_001270952.1
Latest version!
Homo sapiens ubiquitin C-terminal hydrolase L3 (UCHL3), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu56052
NM_006002.4
Latest version!
Homo sapiens ubiquitin C-terminal hydrolase L3 (UCHL3), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu61217 XM_011535212.1
Latest version!
Homo sapiens ubiquitin C-terminal hydrolase L3 (UCHL3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu61218 XM_011535213.2
Latest version!
Homo sapiens ubiquitin C-terminal hydrolase L3 (UCHL3), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu61219 XM_011535214.2
Latest version!
Homo sapiens ubiquitin C-terminal hydrolase L3 (UCHL3), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu84626 XM_017020725.1
Latest version!
Homo sapiens ubiquitin C-terminal hydrolase L3 (UCHL3), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $139.30
$199.00
OHu84627 XM_017020726.1
Latest version!
Homo sapiens ubiquitin C-terminal hydrolase L3 (UCHL3), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $139.30
$199.00
OHu84628 XM_017020727.1
Latest version!
Homo sapiens ubiquitin C-terminal hydrolase L3 (UCHL3), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $139.30
$199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu10261
Clone ID Related Accession (Same CDS sequence) NM_001270952.1
Accession Version NM_001270952.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 585bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-04-12
Organism Homo sapiens(Human)
Product ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL137244.28, BQ427804.1, BC018125.2 and AA234624.1. Transcript Variant: This variant (1) represents the longer transcript and encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU945230.1, AA746355.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
ATGGATCCTG AACTCCTTAG CATGGTACCA AGACCAGTCT GTGCAGTCTT ACTTCTCTTT 
CCTATTACAG AAAAGTATGA AGTATTCAGA ACAGAAGAGG AAGAAAAAAT AAAATCTCAG
GGACAAGATG TTACATCATC AGTATATTTC ATGAAGCAAA CAATCAGCAA TGCCTGTGGA
ACAATTGGAC TGATTCATGC TATTGCAAAC AATAAAGACA AGATGCACTT TGAATCTGGA
TCAACCTTGA AAAAATTCCT GGAGGAATCT GTGTCAATGA GCCCTGAAGA ACGAGCCAGA
TACCTGGAGA ACTATGATGC CATCCGAGTT ACTCATGAGA CCAGTGCCCA TGAAGGTCAG
ACTGAGGCAC CAAGTATAGA TGAGAAAGTA GATCTTCATT TTATTGCATT AGTTCATGTA
GATGGGCATC TCTATGAATT AGATGGGCGG AAGCCATTTC CAATTAACCA TGGTGAAACT
AGTGATGAAA CTTTATTAGA GGATGCCATA GAAGTTTGCA AGAAGTTTAT GGAGCGCGAC
CCTGATGAAC TAAGATTTAA TGCGATTGCT CTTTCTGCAG CATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001257881.1
CDS258..842
Misc Feature(1)159..161
Misc Feature(2)258..830
Misc Feature(3)order(414..416,432..434,654..656,699..701)
Misc Feature(4)order(414..416,426..428,432..434,651..656)
Exon (1)1..191
Exon (2)192..203
Exon (3)204..332
Exon (4)333..489
Exon (5)490..575
Exon (6)576..623
Exon (7)624..699
Exon (8)700..758
Exon (9)759..1050
Translation

Target ORF information:

RefSeq Version NM_001270952.1
Organism Homo sapiens(Human)
Definition Homo sapiens ubiquitin C-terminal hydrolase L3 (UCHL3), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001270952.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGATCCTG AACTCCTTAG CATGGTACCA AGACCAGTCT GTGCAGTCTT ACTTCTCTTT 
CCTATTACAG AAAAGTATGA AGTATTCAGA ACAGAAGAGG AAGAAAAAAT AAAATCTCAG
GGACAAGATG TTACATCATC AGTATATTTC ATGAAGCAAA CAATCAGCAA TGCCTGTGGA
ACAATTGGAC TGATTCATGC TATTGCAAAC AATAAAGACA AGATGCACTT TGAATCTGGA
TCAACCTTGA AAAAATTCCT GGAGGAATCT GTGTCAATGA GCCCTGAAGA ACGAGCCAGA
TACCTGGAGA ACTATGATGC CATCCGAGTT ACTCATGAGA CCAGTGCCCA TGAAGGTCAG
ACTGAGGCAC CAAGTATAGA TGAGAAAGTA GATCTTCATT TTATTGCATT AGTTCATGTA
GATGGGCATC TCTATGAATT AGATGGGCGG AAGCCATTTC CAATTAACCA TGGTGAAACT
AGTGATGAAA CTTTATTAGA GGATGCCATA GAAGTTTGCA AGAAGTTTAT GGAGCGCGAC
CCTGATGAAC TAAGATTTAA TGCGATTGCT CTTTCTGCAG CATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56052
Clone ID Related Accession (Same CDS sequence) NM_006002.4
Accession Version NM_006002.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 693bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-04-12
Organism Homo sapiens(Human)
Product ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA820150.1, BC018125.2 and AA234624.1. On Aug 18, 2012 this sequence version replaced NM_006002.3. Transcript Variant: This variant (2) contains an alternate exon in the 5' coding region and initiates translation at an alternate start codon compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is longer than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI602995.1, BC018125.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGGAGGGTC AACGCTGGCT GCCGCTGGAG GCCAATCCCG AGGTCACCAA CCAGTTTCTT 
AAACAATTAG GTCTACATCC TAACTGGCAA TTCGTTGATG TATATGGAAT GGATCCTGAA
CTCCTTAGCA TGGTACCAAG ACCAGTCTGT GCAGTCTTAC TTCTCTTTCC TATTACAGAA
AAGTATGAAG TATTCAGAAC AGAAGAGGAA GAAAAAATAA AATCTCAGGG ACAAGATGTT
ACATCATCAG TATATTTCAT GAAGCAAACA ATCAGCAATG CCTGTGGAAC AATTGGACTG
ATTCATGCTA TTGCAAACAA TAAAGACAAG ATGCACTTTG AATCTGGATC AACCTTGAAA
AAATTCCTGG AGGAATCTGT GTCAATGAGC CCTGAAGAAC GAGCCAGATA CCTGGAGAAC
TATGATGCCA TCCGAGTTAC TCATGAGACC AGTGCCCATG AAGGTCAGAC TGAGGCACCA
AGTATAGATG AGAAAGTAGA TCTTCATTTT ATTGCATTAG TTCATGTAGA TGGGCATCTC
TATGAATTAG ATGGGCGGAA GCCATTTCCA ATTAACCATG GTGAAACTAG TGATGAAACT
TTATTAGAGG ATGCCATAGA AGTTTGCAAG AAGTTTATGG AGCGCGACCC TGATGAACTA
AGATTTAATG CGATTGCTCT TTCTGCAGCA TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005993.1
CDS72..764
Misc Feature(1)87..752
Misc Feature(2)order(93..110,168..179,234..236,354..356,573..575,579..581,708..710)
Misc Feature(3)order(93..95,336..338,348..350,354..356,573..578)
Misc Feature(4)93..110
Misc Feature(5)294..296
Misc Feature(6)order(336..338,354..356,576..578,621..623)
Misc Feature(7)459..461
Misc Feature(8)525..548
Misc Feature(9)621..623
Misc Feature(10)726..743
Exon (1)1..113
Exon (2)114..125
Exon (3)126..254
Exon (4)255..411
Exon (5)412..497
Exon (6)498..545
Exon (7)546..621
Exon (8)622..680
Exon (9)681..972
Translation

Target ORF information:

RefSeq Version NM_006002.4
Organism Homo sapiens(Human)
Definition Homo sapiens ubiquitin C-terminal hydrolase L3 (UCHL3), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_006002.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGAGGGTC AACGCTGGCT GCCGCTGGAG GCCAATCCCG AGGTCACCAA CCAGTTTCTT 
AAACAATTAG GTCTACATCC TAACTGGCAA TTCGTTGATG TATATGGAAT GGATCCTGAA
CTCCTTAGCA TGGTACCAAG ACCAGTCTGT GCAGTCTTAC TTCTCTTTCC TATTACAGAA
AAGTATGAAG TATTCAGAAC AGAAGAGGAA GAAAAAATAA AATCTCAGGG ACAAGATGTT
ACATCATCAG TATATTTCAT GAAGCAAACA ATCAGCAATG CCTGTGGAAC AATTGGACTG
ATTCATGCTA TTGCAAACAA TAAAGACAAG ATGCACTTTG AATCTGGATC AACCTTGAAA
AAATTCCTGG AGGAATCTGT GTCAATGAGC CCTGAAGAAC GAGCCAGATA CCTGGAGAAC
TATGATGCCA TCCGAGTTAC TCATGAGACC AGTGCCCATG AAGGTCAGAC TGAGGCACCA
AGTATAGATG AGAAAGTAGA TCTTCATTTT ATTGCATTAG TTCATGTAGA TGGGCATCTC
TATGAATTAG ATGGGCGGAA GCCATTTCCA ATTAACCATG GTGAAACTAG TGATGAAACT
TTATTAGAGG ATGCCATAGA AGTTTGCAAG AAGTTTATGG AGCGCGACCC TGATGAACTA
AGATTTAATG CGATTGCTCT TTCTGCAGCA TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61217
Clone ID Related Accession (Same CDS sequence) XM_011535212.1
Accession Version XM_011535212.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 885bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGAGGGTC AACGCTGGCT GCCGCTGGAG GCCAATCCCG AGGTCACCAA CCAGTTTCTT 
AAACAATTAG GTCTACATCC TAACTGGCAA TTCGTTGATG TATATGGAAT GGATCCTGAA
CTCCTTAGCA TGGTACCAAG ACCAGTCTGT GCAGTCTTAC TTCTCTTTCC TATTACAGAA
AAGTATGAAG TATTCAGAAC AGAAGAGGAA GAAAAAATAA AATCTCAGGG ACAAGATGTT
ACATCATCAG TATATTTCAT GAAGCAAACA ATCAGCAATG CCTGTGGAAC AATTGGACTG
ATTCATGCTA TTGCAAACAA TAAAGACAAG ATGCACTTTG AATCTGGATC AACCTTGAAA
AAATTCCTGG AGGAATCTGT GTCAATGAGC CCTGAAGAAC GAGCCAGATA CCTGGAGAAC
TATGATGCCA TCCGAGTTAC TCATGAGACC AGTGCCCATG AAGGTCAGAC TGAGTCCTCC
TCGCCATCCT CATCACAGCC TCATTCTAGC CACTGCAGAA CGAAGGCCTC ATCATTGTGT
CACCATGCAT CCCTGCCCTG GGTCAAACGT AACCATGTAG GCCCTGCAAA GGCTACCAGT
CCATCTCTCC GTCTTCGTCG CTGGCGACCC TTCTTACGTC TACCATCTTT GGGTCTCCAT
TCTGTTGCAC CAAGTATAGA TGAGAAAGTA GATCTTCATT TTATTGCATT AGTTCATGTA
GATGGGCATC TCTATGAATT AGATGGGCGG AAGCCATTTC CAATTAACCA TGGTGAAACT
AGTGATGAAA CTTTATTAGA GGATGCCATA GAAGTTTGCA AGAAGTTTAT GGAGCGCGAC
CCTGATGAAC TAAGATTTAA TGCGATTGCT CTTTCTGCAG CATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011533514.1
CDS72..956
Misc Feature(1)87..944
Misc Feature(2)order(93..110,168..179,234..236,354..356,765..767,771..773,900..902)
Misc Feature(3)order(93..95,336..338,348..350,354..356,765..770)
Misc Feature(4)order(336..338,354..356,768..770,813..815)
Translation

Target ORF information:

RefSeq Version XM_011535212.1
Organism Homo sapiens(Human)
Definition Homo sapiens ubiquitin C-terminal hydrolase L3 (UCHL3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011535212.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGAGGGTC AACGCTGGCT GCCGCTGGAG GCCAATCCCG AGGTCACCAA CCAGTTTCTT 
AAACAATTAG GTCTACATCC TAACTGGCAA TTCGTTGATG TATATGGAAT GGATCCTGAA
CTCCTTAGCA TGGTACCAAG ACCAGTCTGT GCAGTCTTAC TTCTCTTTCC TATTACAGAA
AAGTATGAAG TATTCAGAAC AGAAGAGGAA GAAAAAATAA AATCTCAGGG ACAAGATGTT
ACATCATCAG TATATTTCAT GAAGCAAACA ATCAGCAATG CCTGTGGAAC AATTGGACTG
ATTCATGCTA TTGCAAACAA TAAAGACAAG ATGCACTTTG AATCTGGATC AACCTTGAAA
AAATTCCTGG AGGAATCTGT GTCAATGAGC CCTGAAGAAC GAGCCAGATA CCTGGAGAAC
TATGATGCCA TCCGAGTTAC TCATGAGACC AGTGCCCATG AAGGTCAGAC TGAGTCCTCC
TCGCCATCCT CATCACAGCC TCATTCTAGC CACTGCAGAA CGAAGGCCTC ATCATTGTGT
CACCATGCAT CCCTGCCCTG GGTCAAACGT AACCATGTAG GCCCTGCAAA GGCTACCAGT
CCATCTCTCC GTCTTCGTCG CTGGCGACCC TTCTTACGTC TACCATCTTT GGGTCTCCAT
TCTGTTGCAC CAAGTATAGA TGAGAAAGTA GATCTTCATT TTATTGCATT AGTTCATGTA
GATGGGCATC TCTATGAATT AGATGGGCGG AAGCCATTTC CAATTAACCA TGGTGAAACT
AGTGATGAAA CTTTATTAGA GGATGCCATA GAAGTTTGCA AGAAGTTTAT GGAGCGCGAC
CCTGATGAAC TAAGATTTAA TGCGATTGCT CTTTCTGCAG CATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61218
Clone ID Related Accession (Same CDS sequence) XM_011535213.2
Accession Version XM_011535213.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 873bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535213.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGAGGGTC AACGCTGGCT GCCGCTGGAG GCCAATCCCG AGTTTCTTAA ACAATTAGGT 
CTACATCCTA ACTGGCAATT CGTTGATGTA TATGGAATGG ATCCTGAACT CCTTAGCATG
GTACCAAGAC CAGTCTGTGC AGTCTTACTT CTCTTTCCTA TTACAGAAAA GTATGAAGTA
TTCAGAACAG AAGAGGAAGA AAAAATAAAA TCTCAGGGAC AAGATGTTAC ATCATCAGTA
TATTTCATGA AGCAAACAAT CAGCAATGCC TGTGGAACAA TTGGACTGAT TCATGCTATT
GCAAACAATA AAGACAAGAT GCACTTTGAA TCTGGATCAA CCTTGAAAAA ATTCCTGGAG
GAATCTGTGT CAATGAGCCC TGAAGAACGA GCCAGATACC TGGAGAACTA TGATGCCATC
CGAGTTACTC ATGAGACCAG TGCCCATGAA GGTCAGACTG AGTCCTCCTC GCCATCCTCA
TCACAGCCTC ATTCTAGCCA CTGCAGAACG AAGGCCTCAT CATTGTGTCA CCATGCATCC
CTGCCCTGGG TCAAACGTAA CCATGTAGGC CCTGCAAAGG CTACCAGTCC ATCTCTCCGT
CTTCGTCGCT GGCGACCCTT CTTACGTCTA CCATCTTTGG GTCTCCATTC TGTTGCACCA
AGTATAGATG AGAAAGTAGA TCTTCATTTT ATTGCATTAG TTCATGTAGA TGGGCATCTC
TATGAATTAG ATGGGCGGAA GCCATTTCCA ATTAACCATG GTGAAACTAG TGATGAAACT
TTATTAGAGG ATGCCATAGA AGTTTGCAAG AAGTTTATGG AGCGCGACCC TGATGAACTA
AGATTTAATG CGATTGCTCT TTCTGCAGCA TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011533515.1
CDS65..937
Misc Feature(1)80..925
Misc Feature(2)order(86..103,149..160,215..217,335..337,746..748,752..754,881..883)
Misc Feature(3)order(86..88,317..319,329..331,335..337,746..751)
Misc Feature(4)order(317..319,335..337,749..751,794..796)
Translation

Target ORF information:

RefSeq Version XM_011535213.2
Organism Homo sapiens(Human)
Definition Homo sapiens ubiquitin C-terminal hydrolase L3 (UCHL3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011535213.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGAGGGTC AACGCTGGCT GCCGCTGGAG GCCAATCCCG AGTTTCTTAA ACAATTAGGT 
CTACATCCTA ACTGGCAATT CGTTGATGTA TATGGAATGG ATCCTGAACT CCTTAGCATG
GTACCAAGAC CAGTCTGTGC AGTCTTACTT CTCTTTCCTA TTACAGAAAA GTATGAAGTA
TTCAGAACAG AAGAGGAAGA AAAAATAAAA TCTCAGGGAC AAGATGTTAC ATCATCAGTA
TATTTCATGA AGCAAACAAT CAGCAATGCC TGTGGAACAA TTGGACTGAT TCATGCTATT
GCAAACAATA AAGACAAGAT GCACTTTGAA TCTGGATCAA CCTTGAAAAA ATTCCTGGAG
GAATCTGTGT CAATGAGCCC TGAAGAACGA GCCAGATACC TGGAGAACTA TGATGCCATC
CGAGTTACTC ATGAGACCAG TGCCCATGAA GGTCAGACTG AGTCCTCCTC GCCATCCTCA
TCACAGCCTC ATTCTAGCCA CTGCAGAACG AAGGCCTCAT CATTGTGTCA CCATGCATCC
CTGCCCTGGG TCAAACGTAA CCATGTAGGC CCTGCAAAGG CTACCAGTCC ATCTCTCCGT
CTTCGTCGCT GGCGACCCTT CTTACGTCTA CCATCTTTGG GTCTCCATTC TGTTGCACCA
AGTATAGATG AGAAAGTAGA TCTTCATTTT ATTGCATTAG TTCATGTAGA TGGGCATCTC
TATGAATTAG ATGGGCGGAA GCCATTTCCA ATTAACCATG GTGAAACTAG TGATGAAACT
TTATTAGAGG ATGCCATAGA AGTTTGCAAG AAGTTTATGG AGCGCGACCC TGATGAACTA
AGATTTAATG CGATTGCTCT TTCTGCAGCA TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61219
Clone ID Related Accession (Same CDS sequence) XM_011535214.2
Accession Version XM_011535214.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 777bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535214.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGATCCTG AACTCCTTAG CATGGTACCA AGACCAGTCT GTGCAGTCTT ACTTCTCTTT 
CCTATTACAG AAAAGTATGA AGTATTCAGA ACAGAAGAGG AAGAAAAAAT AAAATCTCAG
GGACAAGATG TTACATCATC AGTATATTTC ATGAAGCAAA CAATCAGCAA TGCCTGTGGA
ACAATTGGAC TGATTCATGC TATTGCAAAC AATAAAGACA AGATGCACTT TGAATCTGGA
TCAACCTTGA AAAAATTCCT GGAGGAATCT GTGTCAATGA GCCCTGAAGA ACGAGCCAGA
TACCTGGAGA ACTATGATGC CATCCGAGTT ACTCATGAGA CCAGTGCCCA TGAAGGTCAG
ACTGAGTCCT CCTCGCCATC CTCATCACAG CCTCATTCTA GCCACTGCAG AACGAAGGCC
TCATCATTGT GTCACCATGC ATCCCTGCCC TGGGTCAAAC GTAACCATGT AGGCCCTGCA
AAGGCTACCA GTCCATCTCT CCGTCTTCGT CGCTGGCGAC CCTTCTTACG TCTACCATCT
TTGGGTCTCC ATTCTGTTGC ACCAAGTATA GATGAGAAAG TAGATCTTCA TTTTATTGCA
TTAGTTCATG TAGATGGGCA TCTCTATGAA TTAGATGGGC GGAAGCCATT TCCAATTAAC
CATGGTGAAA CTAGTGATGA AACTTTATTA GAGGATGCCA TAGAAGTTTG CAAGAAGTTT
ATGGAGCGCG ACCCTGATGA ACTAAGATTT AATGCGATTG CTCTTTCTGC AGCATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011533516.1
CDS248..1024
Misc Feature(1)248..1012
Misc Feature(2)order(404..406,422..424,836..838,881..883)
Misc Feature(3)order(404..406,416..418,422..424,833..838)
Translation

Target ORF information:

RefSeq Version XM_011535214.2
Organism Homo sapiens(Human)
Definition Homo sapiens ubiquitin C-terminal hydrolase L3 (UCHL3), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011535214.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGATCCTG AACTCCTTAG CATGGTACCA AGACCAGTCT GTGCAGTCTT ACTTCTCTTT 
CCTATTACAG AAAAGTATGA AGTATTCAGA ACAGAAGAGG AAGAAAAAAT AAAATCTCAG
GGACAAGATG TTACATCATC AGTATATTTC ATGAAGCAAA CAATCAGCAA TGCCTGTGGA
ACAATTGGAC TGATTCATGC TATTGCAAAC AATAAAGACA AGATGCACTT TGAATCTGGA
TCAACCTTGA AAAAATTCCT GGAGGAATCT GTGTCAATGA GCCCTGAAGA ACGAGCCAGA
TACCTGGAGA ACTATGATGC CATCCGAGTT ACTCATGAGA CCAGTGCCCA TGAAGGTCAG
ACTGAGTCCT CCTCGCCATC CTCATCACAG CCTCATTCTA GCCACTGCAG AACGAAGGCC
TCATCATTGT GTCACCATGC ATCCCTGCCC TGGGTCAAAC GTAACCATGT AGGCCCTGCA
AAGGCTACCA GTCCATCTCT CCGTCTTCGT CGCTGGCGAC CCTTCTTACG TCTACCATCT
TTGGGTCTCC ATTCTGTTGC ACCAAGTATA GATGAGAAAG TAGATCTTCA TTTTATTGCA
TTAGTTCATG TAGATGGGCA TCTCTATGAA TTAGATGGGC GGAAGCCATT TCCAATTAAC
CATGGTGAAA CTAGTGATGA AACTTTATTA GAGGATGCCA TAGAAGTTTG CAAGAAGTTT
ATGGAGCGCG ACCCTGATGA ACTAAGATTT AATGCGATTG CTCTTTCTGC AGCATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu84626
Clone ID Related Accession (Same CDS sequence) XM_017020725.1
Accession Version XM_017020725.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 786bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGAGGGTC AACGCTGGCT GCCGCTGGAG GCCAATCCCG AGGTCACCAA CCAGTTTCTT 
AAACAATTAG GTCTACATCC TAACTGGCAA TTCGTTGATG TATATGGAAT GGATCCTGAA
CTCCTTAGCA TGGTACCAAG ACCAGTCTGT GCAGTCTTAC TTCTCTTTCC TATTACAGAA
AAGTATGAAG TATTCAGAAC AGAAGAGGAA GAAAAAATAA AATCTCAGGG ACAAGATGTT
ACATCATCAG TATATTTCAT GAAGCAAACA ATCAGCAATG CCTGTGGAAC AATTGGACTG
ATTCATGCTA TTGCAAACAA TAAAGACAAG ATGCACTTTG AATCTGGATC AACCTTGAAA
AAATTCCTGG AGGAATCTGT GTCAATGAGC CCTGAAGAAC GAGCCAGATA CCTGGAGAAC
TATGATGCCA TCCGAGTTAC TCATGAGACC AGTGCCCATG AAGGTCAGAC TGAGTCCTCC
TCGCCATCCT CATCACAGCC TCATTCTAGC CACTGCAGAA CGAAGGCCTC ATCATTGTGT
CACCATGCAT CCCTGCCCTG GGTCAAACGT AACCATGTAG GCCCTGCAAA GGCTACCAGT
CCATCTCTCC GTCTTCGTCG CTGGCGACCC TTCTTACGTC TACCATCTTT GGGTCTCCAT
TCTGTTGCAC CAAGTATAGA TGAGAAAGTA GATCTTCATT TTATTGCATT AGTTCATGTA
GATGGGCATC TCTATGAATT AGGAAATTTC CACAGCCACC CCAATCTTCA GCAACCGCCG
TCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016876214.1
CDS72..857
Translation

Target ORF information:

RefSeq Version XM_017020725.1
Organism Homo sapiens(Human)
Definition Homo sapiens ubiquitin C-terminal hydrolase L3 (UCHL3), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017020725.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGAGGGTC AACGCTGGCT GCCGCTGGAG GCCAATCCCG AGGTCACCAA CCAGTTTCTT 
AAACAATTAG GTCTACATCC TAACTGGCAA TTCGTTGATG TATATGGAAT GGATCCTGAA
CTCCTTAGCA TGGTACCAAG ACCAGTCTGT GCAGTCTTAC TTCTCTTTCC TATTACAGAA
AAGTATGAAG TATTCAGAAC AGAAGAGGAA GAAAAAATAA AATCTCAGGG ACAAGATGTT
ACATCATCAG TATATTTCAT GAAGCAAACA ATCAGCAATG CCTGTGGAAC AATTGGACTG
ATTCATGCTA TTGCAAACAA TAAAGACAAG ATGCACTTTG AATCTGGATC AACCTTGAAA
AAATTCCTGG AGGAATCTGT GTCAATGAGC CCTGAAGAAC GAGCCAGATA CCTGGAGAAC
TATGATGCCA TCCGAGTTAC TCATGAGACC AGTGCCCATG AAGGTCAGAC TGAGTCCTCC
TCGCCATCCT CATCACAGCC TCATTCTAGC CACTGCAGAA CGAAGGCCTC ATCATTGTGT
CACCATGCAT CCCTGCCCTG GGTCAAACGT AACCATGTAG GCCCTGCAAA GGCTACCAGT
CCATCTCTCC GTCTTCGTCG CTGGCGACCC TTCTTACGTC TACCATCTTT GGGTCTCCAT
TCTGTTGCAC CAAGTATAGA TGAGAAAGTA GATCTTCATT TTATTGCATT AGTTCATGTA
GATGGGCATC TCTATGAATT AGGAAATTTC CACAGCCACC CCAATCTTCA GCAACCGCCG
TCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu84627
Clone ID Related Accession (Same CDS sequence) XM_017020726.1
Accession Version XM_017020726.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 759bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGAGGGTC AACGCTGGCT GCCGCTGGAG GCCAATCCCG AGGTCACCAA CCAGTTTCTT 
AAACAATTAG GTCTACATCC TAACTGGCAA TTCGTTGATG TATATGGAAT GGATCCTGAA
CTCCTTAGCA TGGTACCAAG ACCAGTCTGT GCAGTCTTAC TTCTCTTTCC TATTACAGAA
AAGTATGAAG TATTCAGAAC AGAAGAGGAA GAAAAAATAA AATCTCAGGG ACAAGATGTT
ACATCATCAG TATATTTCAT GAAGCAAACA ATCAGCAATG CCTGTGGAAC AATTGGACTG
ATTCATGCTA TTGCAAACAA TAAAGACAAG ATGCACTTTG AATCTGGATC AACCTTGAAA
AAATTCCTGG AGGAATCTGT GTCAATGAGC CCTGAAGAAC GAGCCAGATA CCTGGAGAAC
TATGATGCCA TCCGAGTTAC TCATGAGACC AGTGCCCATG AAGGTCAGAC TGAGTCCTCC
TCGCCATCCT CATCACAGCC TCATTCTAGC CACTGCAGAA CGAAGGCCTC ATCATTGTGT
CACCATGCAT CCCTGCCCTG GGTCAAACGT AACCATGTAG GCCCTGCAAA GGCTACCAGT
CCATCTCTCC GTCTTCGTCG CTGGCGACCC TTCTTACGTC TACCATCTTT GGGTCTCCAT
TCTGTTGCAC CAAGTATAGA TGAGAAAGTA GATCTTCATT TTATTGCATT AGTTCATGTA
GATGGGCATC TCTATGAATT AGACCTTCTC GAGTTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016876215.1
CDS72..830
Translation

Target ORF information:

RefSeq Version XM_017020726.1
Organism Homo sapiens(Human)
Definition Homo sapiens ubiquitin C-terminal hydrolase L3 (UCHL3), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017020726.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGAGGGTC AACGCTGGCT GCCGCTGGAG GCCAATCCCG AGGTCACCAA CCAGTTTCTT 
AAACAATTAG GTCTACATCC TAACTGGCAA TTCGTTGATG TATATGGAAT GGATCCTGAA
CTCCTTAGCA TGGTACCAAG ACCAGTCTGT GCAGTCTTAC TTCTCTTTCC TATTACAGAA
AAGTATGAAG TATTCAGAAC AGAAGAGGAA GAAAAAATAA AATCTCAGGG ACAAGATGTT
ACATCATCAG TATATTTCAT GAAGCAAACA ATCAGCAATG CCTGTGGAAC AATTGGACTG
ATTCATGCTA TTGCAAACAA TAAAGACAAG ATGCACTTTG AATCTGGATC AACCTTGAAA
AAATTCCTGG AGGAATCTGT GTCAATGAGC CCTGAAGAAC GAGCCAGATA CCTGGAGAAC
TATGATGCCA TCCGAGTTAC TCATGAGACC AGTGCCCATG AAGGTCAGAC TGAGTCCTCC
TCGCCATCCT CATCACAGCC TCATTCTAGC CACTGCAGAA CGAAGGCCTC ATCATTGTGT
CACCATGCAT CCCTGCCCTG GGTCAAACGT AACCATGTAG GCCCTGCAAA GGCTACCAGT
CCATCTCTCC GTCTTCGTCG CTGGCGACCC TTCTTACGTC TACCATCTTT GGGTCTCCAT
TCTGTTGCAC CAAGTATAGA TGAGAAAGTA GATCTTCATT TTATTGCATT AGTTCATGTA
GATGGGCATC TCTATGAATT AGACCTTCTC GAGTTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu84628
Clone ID Related Accession (Same CDS sequence) XM_017020727.1
Accession Version XM_017020727.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 681bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGGAGGGTC AACGCTGGCT GCCGCTGGAG GCCAATCCCG AGTTTCTTAA ACAATTAGGT 
CTACATCCTA ACTGGCAATT CGTTGATGTA TATGGAATGG ATCCTGAACT CCTTAGCATG
GTACCAAGAC CAGTCTGTGC AGTCTTACTT CTCTTTCCTA TTACAGAAAA GTATGAAGTA
TTCAGAACAG AAGAGGAAGA AAAAATAAAA TCTCAGGGAC AAGATGTTAC ATCATCAGTA
TATTTCATGA AGCAAACAAT CAGCAATGCC TGTGGAACAA TTGGACTGAT TCATGCTATT
GCAAACAATA AAGACAAGAT GCACTTTGAA TCTGGATCAA CCTTGAAAAA ATTCCTGGAG
GAATCTGTGT CAATGAGCCC TGAAGAACGA GCCAGATACC TGGAGAACTA TGATGCCATC
CGAGTTACTC ATGAGACCAG TGCCCATGAA GGTCAGACTG AGGCACCAAG TATAGATGAG
AAAGTAGATC TTCATTTTAT TGCATTAGTT CATGTAGATG GGCATCTCTA TGAATTAGAT
GGGCGGAAGC CATTTCCAAT TAACCATGGT GAAACTAGTG ATGAAACTTT ATTAGAGGAT
GCCATAGAAG TTTGCAAGAA GTTTATGGAG CGCGACCCTG ATGAACTAAG ATTTAATGCG
ATTGCTCTTT CTGCAGCATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016876216.1
CDS59..739
Misc Feature(1)74..727
Misc Feature(2)order(80..97,143..154,209..211,329..331,548..550,554..556,683..685)
Misc Feature(3)order(80..82,311..313,323..325,329..331,548..553)
Misc Feature(4)order(311..313,329..331,551..553,596..598)
Translation

Target ORF information:

RefSeq Version XM_017020727.1
Organism Homo sapiens(Human)
Definition Homo sapiens ubiquitin C-terminal hydrolase L3 (UCHL3), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017020727.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGAGGGTC AACGCTGGCT GCCGCTGGAG GCCAATCCCG AGTTTCTTAA ACAATTAGGT 
CTACATCCTA ACTGGCAATT CGTTGATGTA TATGGAATGG ATCCTGAACT CCTTAGCATG
GTACCAAGAC CAGTCTGTGC AGTCTTACTT CTCTTTCCTA TTACAGAAAA GTATGAAGTA
TTCAGAACAG AAGAGGAAGA AAAAATAAAA TCTCAGGGAC AAGATGTTAC ATCATCAGTA
TATTTCATGA AGCAAACAAT CAGCAATGCC TGTGGAACAA TTGGACTGAT TCATGCTATT
GCAAACAATA AAGACAAGAT GCACTTTGAA TCTGGATCAA CCTTGAAAAA ATTCCTGGAG
GAATCTGTGT CAATGAGCCC TGAAGAACGA GCCAGATACC TGGAGAACTA TGATGCCATC
CGAGTTACTC ATGAGACCAG TGCCCATGAA GGTCAGACTG AGGCACCAAG TATAGATGAG
AAAGTAGATC TTCATTTTAT TGCATTAGTT CATGTAGATG GGCATCTCTA TGAATTAGAT
GGGCGGAAGC CATTTCCAAT TAACCATGGT GAAACTAGTG ATGAAACTTT ATTAGAGGAT
GCCATAGAAG TTTGCAAGAA GTTTATGGAG CGCGACCCTG ATGAACTAAG ATTTAATGCG
ATTGCTCTTT CTGCAGCATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Characterizing substrate selectivity of ubiquitin C-terminal hydrolase-L3 using engineered α-linked ubiquitin substrates.
Biochemistry53(51)8031-42(2014 Dec)
Navarro MF,Carmody L,Romo-Fewell O,Lokensgard ME,Love JJ


book

Ubiquitin C-terminal hydrolase-L3 regulates EMT process and cancer metastasis in prostate cell lines.
Biochemical and biophysical research communications452(3)722-7(2014 Sep)
Song HM,Lee JE,Kim JH


book

Genome-wide association study in a Chinese population with diabetic retinopathy.
Human molecular genetics22(15)3165-73(2013 Aug)
Sheu WH,Kuo JZ,Lee IT,Hung YJ,Lee WJ,Tsai HY,Wang JS,Goodarzi MO,Klein R,Klein BE,Ipp E,Lin SY,Guo X,Hsieh CH,Taylor KD,Fu CP,Rotter JI,Chen YD


book

A novel RNAi lethality rescue screen to identify regulators of adipogenesis.
PloS one7(6)e37680(2012)
van Beekum O,Gao Y,Berger R,Koppen A,Kalkhoven E


book

Structure of the ubiquitin hydrolase UCH-L3 complexed with a suicide substrate.
The Journal of biological chemistry280(2)1512-20(2005 Jan)
Misaghi S,Galardy PJ,Meester WJ,Ovaa H,Ploegh HL,Gaudet R


 
*
*
*
*