LOC8026993 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC8026993
Entrez Gene ID	8026993
Full Name	CDC42 binding protein kinase alpha, putative
Gene Type	protein-coding
Organism	Ixodes scapularis(black-legged tick)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029966709.1	XP_029822569.1	serine/threonine-protein kinase MRCK alpha isoform X1
XM_029966712.1	XP_029822572.1	serine/threonine-protein kinase MRCK alpha isoform X4
XM_002406814.1	XP_002406858.1	CDC42 binding protein kinase alpha, putative
XM_029966711.1	XP_029822571.1	serine/threonine-protein kinase MRCK alpha isoform X3
XM_029966710.1	XP_029822570.1	serine/threonine-protein kinase MRCK alpha isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC8026993 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC8026993 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OIa37168	XM_029966709.1 Latest version!	Ixodes scapularis serine/threonine-protein kinase MRCK alpha (LOC8026993), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIa37169	XM_029966710.1 Latest version!	Ixodes scapularis serine/threonine-protein kinase MRCK alpha (LOC8026993), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIa37170	XM_029966711.1 Latest version!	Ixodes scapularis serine/threonine-protein kinase MRCK alpha (LOC8026993), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIa37171	XM_029966712.1 Latest version!	Ixodes scapularis serine/threonine-protein kinase MRCK alpha (LOC8026993), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC8026993 ( XM_029966709.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029822569.1 Ixodes scapularis serine/threonine-protein kinase MRCK alpha (LOC8026993), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OIa37168
Clone ID Related Accession (Same CDS sequence)	XM_029966709.1
Accession Version	XM_029966709.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	serine/threonine-protein kinase MRCK alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021922756.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGA AAGGGGACTG CCGCTGCCCC GAGGAGCGGA TACGCGAGCT CGAACAAATG 
TTCCTCGGAG GGCCCGTGCT GGCCAGCGGC AAGGCCTTCA CCGTCGAGGG CCTCATCGAC 
GTGCTGGTGG TGCTTTACGA CGAGTGCTGC AACTCGTCGC TACGCAAGGA GAAGACAATG 
ACCAACTTCA TCGAGTACGT CAAACCTGTG GTTCAGAGGA TCAAAGACCT GAGGCTGACC 
AAGGACGACT TCGACGTGCT CAAGGTGATA GGAAGGGGAG CGTTTGGAGA GGTGGCTGTG 
GTGCGAGAGC GGAACACGGG CCAAGTGTAC GCCATGAAGA TCCTCAACAA GTGGGAAATG 
CTCAAGAGGG CGGAGACGGC CTGTTTCCAA GAAGAGCGTG ACGTCCTCGT TTTCGGAGAC 
AGGCGGTGGA TCACGAATCT GCACTACTCC TTTCAAGATG AGACCAACTT GTACCTGGTA 
ATGGACTACT ACTGTGGCGG CGACCTGCTC ACGCTGCTGA GCAAGTTCGA GGACAAGCTG 
CCCGAGGAGA TGTCCCGCTT CTACATCACG GAGATGATCC TGGCCGTGGA CTCCATCCAC 
TCGCTCCACT ACGTCCACCG GGACATCAAG CCAGACAACG TGCTGCTGGA CGCCAACGGA 
CACATTCGCC TGGCCGACTT TGGCTCCTGC CTCAGGCTGT GCGACGACGG AACGGTTCAG 
TCCAACGTTG CGGTCGGCAC CCCTGACTAC ATATCTCCCG AAATCCTAAG GGCGATGGAA 
GATGGGCAGG GCAGGTACGG CCCGGAGTGC GACTGGTGGT CCCTGGGCGT CTGCATGTAC 
GAGATGCTGT ACGGGGAGAC GCCTTTCTAC GCCGAGTCCC TCGTGGAGAC CTACGGCAAG 
ATCATGAACC ACAAGAACTG CTTCGACTTC CCGGACGATC CGGGCTGCTC GGTGTCGGAG 
GATGCCAAGG ACCTGATGCG GCGGCTCATC TGCAGCGCGG ACTGCCGGCT GGGTCAGAAC 
GGCCTGGAGG ACTTCAAGCG CCACCCCTGG TTCAAAGGGG TCGACTGGGA CCACGTACGC 
CAGAGCCAGG CGCCCTACAT TCCGGAGGTG AGCAGTCCGA CGGACACCTC CAACTTCGAC 
GTGGACGAGG CCGACCTCAA GCAGCCCGAG TCCGGCCCCC CGAGCGCCAA TGCGGTGTTC 
TCTGGGCTGC ACCTGCCCTT TGTGGGCTTC ACGTACACCC TGGGCAGCCG GCTGGCCCAG 
GGGGAGGAGA CGGCCGTGGA CGACAGCCAC GTCGTCTGCC GCAGGAAGAT CCAGGCCCTG 
GAGCGGGAGA AGCAGGACAT GTGCCAGAGG ATGGCCAGGG ACCTCAAGAG GGGGCTCCCC 
AACTCTGGCG GCGGAGGTGA CGTGGTGCTG CGCAACAGCT CCACCACGGA CGGGGAGGTG 
CGCAAGCTGC AGGACGAGGT GAACACCTTC CGGAAGAAGA ATGCTGAACT GCAGCTAGAG 
CTGGAGCAGC ACCAGCCCCT GTCCAACTCG CACGGCGGCC ACTCCGTCGG GGTGGGCGAC 
GTCAAGGTCA AGGAGCTGGA GAAGGCGCTG CGTCTCGCAC GGGCGGAGAA GGATGACATC 
CACCGCGACC TGACAGAAGC CCAAGAGAAG CTGAAGCAGC AGACAAAGGA ACTGAAGGAT 
GCCCTAGCCC AGAGGAAACT AGCCATGGAT GAATACACCG AAGTCAGCGA CAAGCTGTCC 
GACCTGCGCA CCCAGAAGCA GAAGCTGTCG CGGCAGGTGC GGGACAAGGA GGAGGAACTG 
GAGAAGGCCA TGCACAAGAT CGACAGCCTC CGGCAAGACC TCAGGAAGGC CGACAAGCTG 
CGCAGAGAAC TGGAGGCCCG GGCGGAGGAG ACCCAGGCGG AAGCCCTGAA GGAGCGGCGC 
TTGAGAGAGC GCAGCGACGA GTACGTGCAC CACCTGGAGG GGGAAGTGGA GGCCCTGCGC 
CAATGCCAGC TGGGACGCCT CTCCACCCAG GGCACGGACG TCTCCCAGGA GGCCGCCAGG 
TTGAAGAGTG AGCTGGAGAA CCAAGAGGTG AAGTTCCGGG AGCTGGAGAT TCAGCTGCAG 
ACGAGGCACG CGACCGAGCT GAACTCGGTG CGTGACCAGC TCCGCGAAGC CGACAGCGCC 
CGGGACACCC TCTTCAAGGA GGTGATGATG CTCAAGGAGA AGAATGAAAA GTACCGGGCT 
GAAAGCGCCA TGGAGAGCCA GGAGACCCTG GCGGAGATCA AGAAGGCCCA CGAGCGGGAG 
AAGGCCATGC TCTCCGAAGA GGTCCGCAAG CTCACCCGGG AAGGGGAGCA GCTGGCGGAG 
ACTGTGAGGA GACTGCACGA GGAGCGGAGG TGCTTCGAAG AGGAGATGGG CCAGCTGCGG 
GAGAAGAAGG AGTCCGTCGC TCAGTGGGAG GCACAGATCA GCGAGATCAT CCAGTGGGTG 
AGCGACGAAA AGGATGCCCG TGGCTACCTG CAAGCCCTGG CCACTAAGAT GACGGAGGAG 
CTGGACTACC TGAGGATGAG CGGACTGCCC ACGCCCACTG CGGAGAAGAA CTGGCGGAAC 
CGGCGGTCCC AGAAGCTGGA AAAGATGGAG CTGCTCACCC TGCAGTCGAG CCTCCAGAGC 
GAGATCCAGG CCAAGCAGGT GGTCTGCGAG GAGCTGAGCC GGGTGCGGGC CGAGCTGGTC 
GCCCAGCAGA AGGAGCACCG CAAGATTCAG CAGGAGTTTG AGCACTTCCG GAAGGAGTCT 
ACCAAGAAGG ACTCCCAGAT CCGAGACCTG CAGCAGCGCT TGGAAGCTAC AGGTGACGGA 
TTCCTGGAGC GGCCGTCTTC GCAGATGTCT TTCCTCGACC AGTTCCTCAA GGACACCTCG 
CGGCTCAATC ACAGCGAAAG CGGGGAGTCC GCCGGAGAAG CCGTTGCCAA CGACACGACA 
GAGTCTGAGA TGGAGAACCA GGTGCCGCCG CCGCCCATCG TCAGGTCCAG CACCTCCACT 
GAGGAACGGC GAGGAATCAA AATCTCCTCC CCGATTAGCG ACGCGCGTCC CACAATCACG 
GTTCTCACGC CCAAGCCCAA GGCGCACCAG TTCCTGGTGC GGACGTTTGT GGCGCCCCTC 
AAGTGCAACC ACTGCACCTC CCTCATGGTG GGCCTCATCC GGCAAGGGGC TGTGTGCGAG 
GTGTGTGGCT TTGCCTGCCA CGTCACGTGT CAAGACAAGG TGCCTGCCGT GTGTCCAGTG 
CCAATGGATC AGAGTGAGCA GACCTTCTTT CAGACTGCCG CCTCAAAAAG GCCAGTTGGA 
ATCGACCCAA CAAGAGGCGT GGGAACAGCC TACGAAGGAT ATGTCAAGGT GCCCAAACTG 
GGCGGCGTGA AGAAAGGCTG GCAGAGGCAG TTTGTGGCTG TCTGCGACTT CAAGCTCTTC 
CTGTACGACC TGCTGCAGGA GAAGAACGTC CAGCCGGCGG TGTCTGCCTC CCAGGTGCTC 
GACATGAGGG ACGAGGAATT CTCGGTCACC TCCGTGCTCG AGTCGGACGT GATCCACGCC 
AACCGGAAGG ACATCCCGTG CATCTTTCGA GTGTCGACGT CCATGATGGA TCCCCCCGGG 
CTGCGCAGCC AGACGCTGAT GATGGTGGAC AAGGAGAGCG AGAAGGTCAA GTGGGTGGAC 
GCCCTCAATG AGCTGCACCG CATCCTCCGC CGGAACAAGC TGCCCCACAG GACGGTGTTC 
CAAGCCAAGG AGATTCTGGA CAGCACCCTC GTGATTATCA AGAATGCCTT ATCAGCCACT 
GTGATAGACC CGGACCGGAT AGCGCTGGGA ACCGAGGAGG GGCTGTACTG CGTCGACCTG 
GACCGAGAAG GTGAGGGAGA GATTGCTCGG ATCGGGGACG GCAAGAGGAT CGCTCAGCTC 
GAGTACATTC CTGATGAGCA GTTGCTCATT GCCATAGCAG GCAAGCAGCG ACACTTGCGG 
CTGATTCCCG TGGGGGCACT GGACGGCCAC GAGGTCGACT GGATCAAGGT GGCGGACACC 
AAGGGCTGCT CGAGCTTCTG CACCACCCGC ATGGACCATG GGGGCTCGTC TACTTACTAC 
TTCTGTGTGG CCGTCAAGAA GCAGGTGCTG GTACACCAAG TGAACCGCAC CAAGAGCCGG 
CACGGGGGCC GGCAGGTGAT CAGCGTGCCC GGGGTGGCCC AGTGCCTGGA CGTCTGGCAA 
GACCGCCTCT GCGTGGGGCT GCCCTCCCTC TTCTACCTTT ACAGCCTACA GGGCACCACC 
GAGCCCATCT GCCTGGTGCA GCCGGACAAC AACCTGTCCT TCCTGTACCA CAACCCGATG 
GATGCCTTCA TGGCTGTGGA GCTCCCCAAC AACGAGTACC TACTCGTCTT CAGCCAGCTT 
GGAGTGTTTG TGAACAACCT TGGGAAGAAG ACCCGGGAGA AAGAAGTTAT GTTCTCGGCC 
TTACCGCTGG CAGTCAGTTA CCACGACTCC TACCTGTGCG TCTACATGGA GAGCCACGTG 
GATGTGTACG AGGTGACCTC TGGGGACTGG GTGCAGACCA TGAACCTCAA GAAGACGAAG 
CCCCTGTGCC GGCAGGGCCA GCTGTGCTTC TCGCTGACGA CGGAGGCGCC CCAGGTGCTG 
TACCTGCACC CCGTGCACCG GAGGGAGCAC GCCGTGAGGG TGGAGCCCCA CCGGCCAGGG 
GCCCCCCACG CCCTGGGGCC CCTCAGGGGT TCCTCGTCCT CCGTCGCTGC CTCCCTGGTC 
ACCAGGGCCC GCAGGAGGTT CTCCGTGCGC GAGCAGGAGA AGCTGCCCAA GGGGGACCGC 
CGGTCGAGGA TGATCTCTGC ACCCTCGAAC TTCAACCACA TCCAACACAT GGGACCCAGC 
GAGCTCCAGA AACTCATCGA CCTGCCCACG ACGGCACAGG GGGTTGCAGC CATGGAGGAG 
AAGAGGGCTT GGGTGAAGTC TGTGAAACCT CCAGGTCTGT TTCAAGCGTC ACACCTCGAA 
AGCAGAACCC AAGGGGTGCC CCTGCAGCCC AAGCGGCCCG CCCCCCTGGC TCCCGGCCAC 
AGAACTGCCA AGTCGTCGCT GACAGCCTCT CCCGACGGAT CGCTCTCTTC TCAGGAGCAC 
ACCTCCGGTC CGCAGGCCTC CTCGGCTCAC GACGACTCTG GGCACCAGTC GAGTCCGCGG 
CACTCCATCG CCTCCAACAA CAGCTCCAAC TTGAGCAGCC CCCCAAGTCC GGTGGATCTC 
GGCCTTGGCT TGGAAGATGC GCGCGAGCAG TTCTCCTCGA GCTACGAGTC GCAGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029822569.1
CDS	650..5929
Translation	MSKKGDCRCPEERIRELEQMFLGGPVLASGKAFTVEGLIDVLVVLYDECCNSSLRKEKTMTNFIEYVKPVVQRIKDLRLTKDDFDVLKVIGRGAFGEVAVVRERNTGQVYAMKILNKWEMLKRAETACFQEERDVLVFGDRRWITNLHYSFQDETNLYLVMDYYCGGDLLTLLSKFEDKLPEEMSRFYITEMILAVDSIHSLHYVHRDIKPDNVLLDANGHIRLADFGSCLRLCDDGTVQSNVAVGTPDYISPEILRAMEDGQGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKNCFDFPDDPGCSVSEDAKDLMRRLICSADCRLGQNGLEDFKRHPWFKGVDWDHVRQSQAPYIPEVSSPTDTSNFDVDEADLKQPESGPPSANAVFSGLHLPFVGFTYTLGSRLAQGEETAVDDSHVVCRRKIQALEREKQDMCQRMARDLKRGLPNSGGGGDVVLRNSSTTDGEVRKLQDEVNTFRKKNAELQLELEQHQPLSNSHGGHSVGVGDVKVKELEKALRLARAEKDDIHRDLTEAQEKLKQQTKELKDALAQRKLAMDEYTEVSDKLSDLRTQKQKLSRQVRDKEEELEKAMHKIDSLRQDLRKADKLRRELEARAEETQAEALKERRLRERSDEYVHHLEGEVEALRQCQLGRLSTQGTDVSQEAARLKSELENQEVKFRELEIQLQTRHATELNSVRDQLREADSARDTLFKEVMMLKEKNEKYRAESAMESQETLAEIKKAHEREKAMLSEEVRKLTREGEQLAETVRRLHEERRCFEEEMGQLREKKESVAQWEAQISEIIQWVSDEKDARGYLQALATKMTEELDYLRMSGLPTPTAEKNWRNRRSQKLEKMELLTLQSSLQSEIQAKQVVCEELSRVRAELVAQQKEHRKIQQEFEHFRKESTKKDSQIRDLQQRLEATGDGFLERPSSQMSFLDQFLKDTSRLNHSESGESAGEAVANDTTESEMENQVPPPPIVRSSTSTEERRGIKISSPISDARPTITVLTPKPKAHQFLVRTFVAPLKCNHCTSLMVGLIRQGAVCEVCGFACHVTCQDKVPAVCPVPMDQSEQTFFQTAASKRPVGIDPTRGVGTAYEGYVKVPKLGGVKKGWQRQFVAVCDFKLFLYDLLQEKNVQPAVSASQVLDMRDEEFSVTSVLESDVIHANRKDIPCIFRVSTSMMDPPGLRSQTLMMVDKESEKVKWVDALNELHRILRRNKLPHRTVFQAKEILDSTLVIIKNALSATVIDPDRIALGTEEGLYCVDLDREGEGEIARIGDGKRIAQLEYIPDEQLLIAIAGKQRHLRLIPVGALDGHEVDWIKVADTKGCSSFCTTRMDHGGSSTYYFCVAVKKQVLVHQVNRTKSRHGGRQVISVPGVAQCLDVWQDRLCVGLPSLFYLYSLQGTTEPICLVQPDNNLSFLYHNPMDAFMAVELPNNEYLLVFSQLGVFVNNLGKKTREKEVMFSALPLAVSYHDSYLCVYMESHVDVYEVTSGDWVQTMNLKKTKPLCRQGQLCFSLTTEAPQVLYLHPVHRREHAVRVEPHRPGAPHALGPLRGSSSSVAASLVTRARRRFSVREQEKLPKGDRRSRMISAPSNFNHIQHMGPSELQKLIDLPTTAQGVAAMEEKRAWVKSVKPPGLFQASHLESRTQGVPLQPKRPAPLAPGHRTAKSSLTASPDGSLSSQEHTSGPQASSAHDDSGHQSSPRHSIASNNSSNLSSPPSPVDLGLGLEDAREQFSSSYESQS

Target ORF information:

RefSeq Version	XM_029966709.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis serine/threonine-protein kinase MRCK alpha (LOC8026993), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029966709.1

ORF Insert Sequence:

ATGTCCAAGA AAGGGGACTG CCGCTGCCCC GAGGAGCGGA TACGCGAGCT CGAACAAATG 
TTCCTCGGAG GGCCCGTGCT GGCCAGCGGC AAGGCCTTCA CCGTCGAGGG CCTCATCGAC 
GTGCTGGTGG TGCTTTACGA CGAGTGCTGC AACTCGTCGC TACGCAAGGA GAAGACAATG 
ACCAACTTCA TCGAGTACGT CAAACCTGTG GTTCAGAGGA TCAAAGACCT GAGGCTGACC 
AAGGACGACT TCGACGTGCT CAAGGTGATA GGAAGGGGAG CGTTTGGAGA GGTGGCTGTG 
GTGCGAGAGC GGAACACGGG CCAAGTGTAC GCCATGAAGA TCCTCAACAA GTGGGAAATG 
CTCAAGAGGG CGGAGACGGC CTGTTTCCAA GAAGAGCGTG ACGTCCTCGT TTTCGGAGAC 
AGGCGGTGGA TCACGAATCT GCACTACTCC TTTCAAGATG AGACCAACTT GTACCTGGTA 
ATGGACTACT ACTGTGGCGG CGACCTGCTC ACGCTGCTGA GCAAGTTCGA GGACAAGCTG 
CCCGAGGAGA TGTCCCGCTT CTACATCACG GAGATGATCC TGGCCGTGGA CTCCATCCAC 
TCGCTCCACT ACGTCCACCG GGACATCAAG CCAGACAACG TGCTGCTGGA CGCCAACGGA 
CACATTCGCC TGGCCGACTT TGGCTCCTGC CTCAGGCTGT GCGACGACGG AACGGTTCAG 
TCCAACGTTG CGGTCGGCAC CCCTGACTAC ATATCTCCCG AAATCCTAAG GGCGATGGAA 
GATGGGCAGG GCAGGTACGG CCCGGAGTGC GACTGGTGGT CCCTGGGCGT CTGCATGTAC 
GAGATGCTGT ACGGGGAGAC GCCTTTCTAC GCCGAGTCCC TCGTGGAGAC CTACGGCAAG 
ATCATGAACC ACAAGAACTG CTTCGACTTC CCGGACGATC CGGGCTGCTC GGTGTCGGAG 
GATGCCAAGG ACCTGATGCG GCGGCTCATC TGCAGCGCGG ACTGCCGGCT GGGTCAGAAC 
GGCCTGGAGG ACTTCAAGCG CCACCCCTGG TTCAAAGGGG TCGACTGGGA CCACGTACGC 
CAGAGCCAGG CGCCCTACAT TCCGGAGGTG AGCAGTCCGA CGGACACCTC CAACTTCGAC 
GTGGACGAGG CCGACCTCAA GCAGCCCGAG TCCGGCCCCC CGAGCGCCAA TGCGGTGTTC 
TCTGGGCTGC ACCTGCCCTT TGTGGGCTTC ACGTACACCC TGGGCAGCCG GCTGGCCCAG 
GGGGAGGAGA CGGCCGTGGA CGACAGCCAC GTCGTCTGCC GCAGGAAGAT CCAGGCCCTG 
GAGCGGGAGA AGCAGGACAT GTGCCAGAGG ATGGCCAGGG ACCTCAAGAG GGGGCTCCCC 
AACTCTGGCG GCGGAGGTGA CGTGGTGCTG CGCAACAGCT CCACCACGGA CGGGGAGGTG 
CGCAAGCTGC AGGACGAGGT GAACACCTTC CGGAAGAAGA ATGCTGAACT GCAGCTAGAG 
CTGGAGCAGC ACCAGCCCCT GTCCAACTCG CACGGCGGCC ACTCCGTCGG GGTGGGCGAC 
GTCAAGGTCA AGGAGCTGGA GAAGGCGCTG CGTCTCGCAC GGGCGGAGAA GGATGACATC 
CACCGCGACC TGACAGAAGC CCAAGAGAAG CTGAAGCAGC AGACAAAGGA ACTGAAGGAT 
GCCCTAGCCC AGAGGAAACT AGCCATGGAT GAATACACCG AAGTCAGCGA CAAGCTGTCC 
GACCTGCGCA CCCAGAAGCA GAAGCTGTCG CGGCAGGTGC GGGACAAGGA GGAGGAACTG 
GAGAAGGCCA TGCACAAGAT CGACAGCCTC CGGCAAGACC TCAGGAAGGC CGACAAGCTG 
CGCAGAGAAC TGGAGGCCCG GGCGGAGGAG ACCCAGGCGG AAGCCCTGAA GGAGCGGCGC 
TTGAGAGAGC GCAGCGACGA GTACGTGCAC CACCTGGAGG GGGAAGTGGA GGCCCTGCGC 
CAATGCCAGC TGGGACGCCT CTCCACCCAG GGCACGGACG TCTCCCAGGA GGCCGCCAGG 
TTGAAGAGTG AGCTGGAGAA CCAAGAGGTG AAGTTCCGGG AGCTGGAGAT TCAGCTGCAG 
ACGAGGCACG CGACCGAGCT GAACTCGGTG CGTGACCAGC TCCGCGAAGC CGACAGCGCC 
CGGGACACCC TCTTCAAGGA GGTGATGATG CTCAAGGAGA AGAATGAAAA GTACCGGGCT 
GAAAGCGCCA TGGAGAGCCA GGAGACCCTG GCGGAGATCA AGAAGGCCCA CGAGCGGGAG 
AAGGCCATGC TCTCCGAAGA GGTCCGCAAG CTCACCCGGG AAGGGGAGCA GCTGGCGGAG 
ACTGTGAGGA GACTGCACGA GGAGCGGAGG TGCTTCGAAG AGGAGATGGG CCAGCTGCGG 
GAGAAGAAGG AGTCCGTCGC TCAGTGGGAG GCACAGATCA GCGAGATCAT CCAGTGGGTG 
AGCGACGAAA AGGATGCCCG TGGCTACCTG CAAGCCCTGG CCACTAAGAT GACGGAGGAG 
CTGGACTACC TGAGGATGAG CGGACTGCCC ACGCCCACTG CGGAGAAGAA CTGGCGGAAC 
CGGCGGTCCC AGAAGCTGGA AAAGATGGAG CTGCTCACCC TGCAGTCGAG CCTCCAGAGC 
GAGATCCAGG CCAAGCAGGT GGTCTGCGAG GAGCTGAGCC GGGTGCGGGC CGAGCTGGTC 
GCCCAGCAGA AGGAGCACCG CAAGATTCAG CAGGAGTTTG AGCACTTCCG GAAGGAGTCT 
ACCAAGAAGG ACTCCCAGAT CCGAGACCTG CAGCAGCGCT TGGAAGCTAC AGGTGACGGA 
TTCCTGGAGC GGCCGTCTTC GCAGATGTCT TTCCTCGACC AGTTCCTCAA GGACACCTCG 
CGGCTCAATC ACAGCGAAAG CGGGGAGTCC GCCGGAGAAG CCGTTGCCAA CGACACGACA 
GAGTCTGAGA TGGAGAACCA GGTGCCGCCG CCGCCCATCG TCAGGTCCAG CACCTCCACT 
GAGGAACGGC GAGGAATCAA AATCTCCTCC CCGATTAGCG ACGCGCGTCC CACAATCACG 
GTTCTCACGC CCAAGCCCAA GGCGCACCAG TTCCTGGTGC GGACGTTTGT GGCGCCCCTC 
AAGTGCAACC ACTGCACCTC CCTCATGGTG GGCCTCATCC GGCAAGGGGC TGTGTGCGAG 
GTGTGTGGCT TTGCCTGCCA CGTCACGTGT CAAGACAAGG TGCCTGCCGT GTGTCCAGTG 
CCAATGGATC AGAGTGAGCA GACCTTCTTT CAGACTGCCG CCTCAAAAAG GCCAGTTGGA 
ATCGACCCAA CAAGAGGCGT GGGAACAGCC TACGAAGGAT ATGTCAAGGT GCCCAAACTG 
GGCGGCGTGA AGAAAGGCTG GCAGAGGCAG TTTGTGGCTG TCTGCGACTT CAAGCTCTTC 
CTGTACGACC TGCTGCAGGA GAAGAACGTC CAGCCGGCGG TGTCTGCCTC CCAGGTGCTC 
GACATGAGGG ACGAGGAATT CTCGGTCACC TCCGTGCTCG AGTCGGACGT GATCCACGCC 
AACCGGAAGG ACATCCCGTG CATCTTTCGA GTGTCGACGT CCATGATGGA TCCCCCCGGG 
CTGCGCAGCC AGACGCTGAT GATGGTGGAC AAGGAGAGCG AGAAGGTCAA GTGGGTGGAC 
GCCCTCAATG AGCTGCACCG CATCCTCCGC CGGAACAAGC TGCCCCACAG GACGGTGTTC 
CAAGCCAAGG AGATTCTGGA CAGCACCCTC GTGATTATCA AGAATGCCTT ATCAGCCACT 
GTGATAGACC CGGACCGGAT AGCGCTGGGA ACCGAGGAGG GGCTGTACTG CGTCGACCTG 
GACCGAGAAG GTGAGGGAGA GATTGCTCGG ATCGGGGACG GCAAGAGGAT CGCTCAGCTC 
GAGTACATTC CTGATGAGCA GTTGCTCATT GCCATAGCAG GCAAGCAGCG ACACTTGCGG 
CTGATTCCCG TGGGGGCACT GGACGGCCAC GAGGTCGACT GGATCAAGGT GGCGGACACC 
AAGGGCTGCT CGAGCTTCTG CACCACCCGC ATGGACCATG GGGGCTCGTC TACTTACTAC 
TTCTGTGTGG CCGTCAAGAA GCAGGTGCTG GTACACCAAG TGAACCGCAC CAAGAGCCGG 
CACGGGGGCC GGCAGGTGAT CAGCGTGCCC GGGGTGGCCC AGTGCCTGGA CGTCTGGCAA 
GACCGCCTCT GCGTGGGGCT GCCCTCCCTC TTCTACCTTT ACAGCCTACA GGGCACCACC 
GAGCCCATCT GCCTGGTGCA GCCGGACAAC AACCTGTCCT TCCTGTACCA CAACCCGATG 
GATGCCTTCA TGGCTGTGGA GCTCCCCAAC AACGAGTACC TACTCGTCTT CAGCCAGCTT 
GGAGTGTTTG TGAACAACCT TGGGAAGAAG ACCCGGGAGA AAGAAGTTAT GTTCTCGGCC 
TTACCGCTGG CAGTCAGTTA CCACGACTCC TACCTGTGCG TCTACATGGA GAGCCACGTG 
GATGTGTACG AGGTGACCTC TGGGGACTGG GTGCAGACCA TGAACCTCAA GAAGACGAAG 
CCCCTGTGCC GGCAGGGCCA GCTGTGCTTC TCGCTGACGA CGGAGGCGCC CCAGGTGCTG 
TACCTGCACC CCGTGCACCG GAGGGAGCAC GCCGTGAGGG TGGAGCCCCA CCGGCCAGGG 
GCCCCCCACG CCCTGGGGCC CCTCAGGGGT TCCTCGTCCT CCGTCGCTGC CTCCCTGGTC 
ACCAGGGCCC GCAGGAGGTT CTCCGTGCGC GAGCAGGAGA AGCTGCCCAA GGGGGACCGC 
CGGTCGAGGA TGATCTCTGC ACCCTCGAAC TTCAACCACA TCCAACACAT GGGACCCAGC 
GAGCTCCAGA AACTCATCGA CCTGCCCACG ACGGCACAGG GGGTTGCAGC CATGGAGGAG 
AAGAGGGCTT GGGTGAAGTC TGTGAAACCT CCAGGTCTGT TTCAAGCGTC ACACCTCGAA 
AGCAGAACCC AAGGGGTGCC CCTGCAGCCC AAGCGGCCCG CCCCCCTGGC TCCCGGCCAC 
AGAACTGCCA AGTCGTCGCT GACAGCCTCT CCCGACGGAT CGCTCTCTTC TCAGGAGCAC 
ACCTCCGGTC CGCAGGCCTC CTCGGCTCAC GACGACTCTG GGCACCAGTC GAGTCCGCGG 
CACTCCATCG CCTCCAACAA CAGCTCCAAC TTGAGCAGCC CCCCAAGTCC GGTGGATCTC 
GGCCTTGGCT TGGAAGATGC GCGCGAGCAG TTCTCCTCGA GCTACGAGTC GCAGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8026993 ( XM_029966710.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029822570.1 Ixodes scapularis serine/threonine-protein kinase MRCK alpha (LOC8026993), transcript variant X2, mRNA.

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CloneID	OIa37169
Clone ID Related Accession (Same CDS sequence)	XM_029966710.1
Accession Version	XM_029966710.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	serine/threonine-protein kinase MRCK alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021922756.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGA AAGGGGACTG CCGCTGCCCC GAGGAGCGGA TACGCGAGCT CGAACAAATG 
TTCCTCGGAG GGCCCGTGCT GGCCAGCGGC AAGGCCTTCA CCGTCGAGGG CCTCATCGAC 
GTGCTGGTGG TGCTTTACGA CGAGTGCTGC AACTCGTCGC TACGCAAGGA GAAGACAATG 
ACCAACTTCA TCGAGTACGT CAAACCTGTG GTTCAGAGGA TCAAAGACCT GAGGCTGACC 
AAGGACGACT TCGACGTGCT CAAGGTGATA GGAAGGGGAG CGTTTGGAGA GGTGGCTGTG 
GTGCGAGAGC GGAACACGGG CCAAGTGTAC GCCATGAAGA TCCTCAACAA GTGGGAAATG 
CTCAAGAGGG CGGAGACGGC CTGTTTCCAA GAAGAGCGTG ACGTCCTCGT TTTCGGAGAC 
AGGCGGTGGA TCACGAATCT GCACTACTCC TTTCAAGATG AGACCAACTT GTACCTGGTA 
ATGGACTACT ACTGTGGCGG CGACCTGCTC ACGCTGCTGA GCAAGTTCGA GGACAAGCTG 
CCCGAGGAGA TGTCCCGCTT CTACATCACG GAGATGATCC TGGCCGTGGA CTCCATCCAC 
TCGCTCCACT ACGTCCACCG GGACATCAAG CCAGACAACG TGCTGCTGGA CGCCAACGGA 
CACATTCGCC TGGCCGACTT TGGCTCCTGC CTCAGGCTGT GCGACGACGG AACGGTTCAG 
TCCAACGTTG CGGTCGGCAC CCCTGACTAC ATATCTCCCG AAATCCTAAG GGCGATGGAA 
GATGGGCAGG GCAGGTACGG CCCGGAGTGC GACTGGTGGT CCCTGGGCGT CTGCATGTAC 
GAGATGCTGT ACGGGGAGAC GCCTTTCTAC GCCGAGTCCC TCGTGGAGAC CTACGGCAAG 
ATCATGAACC ACAAGAACTG CTTCGACTTC CCGGACGATC CGGGCTGCTC GGTGTCGGAG 
GATGCCAAGG ACCTGATGCG GCGGCTCATC TGCAGCGCGG ACTGCCGGCT GGGTCAGAAC 
GGCCTGGAGG ACTTCAAGCG CCACCCCTGG TTCAAAGGGG TCGACTGGGA CCACGTACGC 
CAGAGCCAGG CGCCCTACAT TCCGGAGGTG AGCAGTCCGA CGGACACCTC CAACTTCGAC 
GTGGACGAGG CCGACCTCAA GCAGCCCGAG TCCGGCCCCC CGAGCGCCAA TGCGGTGTTC 
TCTGGGCTGC ACCTGCCCTT TGTGGGCTTC ACGTACACCC TGGGCAGCCG GCTGGCCCAG 
GGGGAGGAGA CGGCCGTGGA CGACAGCCAC GTCGTCTGCC GCAGGAAGAT CCAGGCCCTG 
GAGCGGGAGA AGCAGGACAT GTGCCAGAGG ATGGCCAGGG ACCTCAAGAG GGGGCTCCCC 
AACTCTGGCG GCGGAGGTGA CGTGGTGCTG CGCAACAGCT CCACCACGGA CGGGGAGGTG 
CGCAAGCTGC AGGACGAGGT GAACACCTTC CGGAAGAAGA ATGCTGAACT GCAGCTAGAG 
CTGGAGCAGC ACCAGCCCCT GTCCAACTCG CACGGCGGCC ACTCCGTCGG GGTGGGCGAC 
GTCAAGGTCA AGGAGCTGGA GAAGGCGCTG CGTCTCGCAC GGGCGGAGAA GGATGACATC 
CACCGCGACC TGACAGAAGC CCAAGAGAAG CTGAAGCAGC AGACAAAGGA ACTGAAGGAT 
GCCCTAGCCC AGAGGAAACT AGCCATGGAT GAATACACCG AAGTCAGCGA CAAGCTGTCC 
GACCTGCGCA CCCAGAAGCA GAAGCTGTCG CGGCAGGTGC GGGACAAGGA GGAGGAACTG 
GAGAAGGCCA TGCACAAGAT CGACAGCCTC CGGCAAGACC TCAGGAAGGC CGACAAGCTG 
CGCAGAGAAC TGGAGGCCCG GGCGGAGGAG ACCCAGGCGG AAGCCCTGAA GGAGCGGCGC 
TTGAGAGAGC GCAGCGACGA GTACGTGCAC CACCTGGAGG GGGAAGTGGA GGCCCTGCGC 
CAATGCCAGC TGGGACGCCT CTCCACCCAG GGCACGGACG TCTCCCAGGA GGCCGCCAGG 
TTGAAGAGTG AGCTGGAGAA CCAAGAGGTG AAGTTCCGGG AGCTGGAGAT TCAGCTGCAG 
ACGAGGCACG CGACCGAGCT GAACTCGGTG CGTGACCAGC TCCGCGAAGC CGACAGCGCC 
CGGGACACCC TCTTCAAGGA GGTGATGATG CTCAAGGAGA AGAATGAAAA GTACCGGGCT 
GAAAGCGCCA TGGAGAGCCA GGAGACCCTG GCGGAGATCA AGAAGGCCCA CGAGCGGGAG 
AAGGCCATGC TCTCCGAAGA GGTCCGCAAG CTCACCCGGG AAGGGGAGCA GCTGGCGGAG 
ACTGTGAGGA GACTGCACGA GGAGCGGAGG TGCTTCGAAG AGGAGATGGG CCAGCTGCGG 
GAGAAGAAGG AGTCCGTCGC TCAGTGGGAG GCACAGATCA GCGAGATCAT CCAGTGGGTG 
AGCGACGAAA AGGATGCCCG TGGCTACCTG CAAGCCCTGG CCACTAAGAT GACGGAGGAG 
CTGGACTACC TGAGGATGAG CGGACTGCCC ACGCCCACTG CGGAGAAGAA CTGGCGGAAC 
CGGCGGTCCC AGAAGCTGGA AAAGATGGAG CTGCTCACCC TGCAGTCGAG CCTCCAGAGC 
GAGATCCAGG CCAAGCAGGT GGTCTGCGAG GAGCTGAGCC GGGTGCGGGC CGAGCTGGTC 
GCCCAGCAGA AGGAGCACCG CAAGATTCAG CAGGAGTTTG AGCACTTCCG GAAGGAGTCT 
ACCAAGAAGG ACTCCCAGAT CCGAGACCTG CAGCAGCGCT TGGAAGCTAC AGGTGACGGA 
TTCCTGGAGC GGCCGTCTTC GCAGATGTCT TTCCTCGACC AGTTCCTCAA GGACACCTCG 
CGGCTCAATC ACAGCGAAAG CGGGGAGTCC GCCGGAGAAG CCGTTGCCAA CGACACGACA 
GAGTCTGAGA TGGAGAACCA GGTGCCGCCG CCGCCCATCG TCAGGTCCAG CACCTCCACT 
GAGGAACGGC GAGGAATCAA AATCTCCTCC CCGATTAGCG ACGCGCGTCC CACAATCACG 
GTTCTCACGC CCAAGCCCAA GGCGCACCAG TTCCTGGTGC GGACGTTTGT GGCGCCCCTC 
AAGTGCAACC ACTGCACCTC CCTCATGGTG GGCCTCATCC GGCAAGGGGC TGTGTGCGAG 
GTGTGTGGCT TTGCCTGCCA CGTCACGTGT CAAGACAAGG TGCCTGCCGT GTGTCCAGTG 
CCAATGGATC AGAGTGAGCA GACCTTCTTT CAGACTGCCG CCTCAAAAAG GCCAGTTGGA 
ATCGACCCAA CAAGAGGCGT GGGAACAGCC TACGAAGGAT ATGTCAAGGT GCCCAAACTG 
GGCGGCGTGA AGAAAGGCTG GCAGAGGCAG TTTGTGGCTG TCTGCGACTT CAAGCTCTTC 
CTGTACGACC TGCTGCAGGA GAAGAACGTC CAGCCGGCGG TGTCTGCCTC CCAGGTGCTC 
GACATGAGGG ACGAGGAATT CTCGGTCACC TCCGTGCTCG AGTCGGACGT GATCCACGCC 
AACCGGAAGG ACATCCCGTG CATCTTTCGA GTGTCGACGT CCATGATGGA TCCCCCCGGG 
CTGCGCAGCC AGACGCTGAT GATGGTGGAC AAGGAGAGCG AGAAGGTCAA GTGGGTGGAC 
GCCCTCAATG AGCTGCACCG CATCCTCCGC CGGAACAAGC TGCCCCACAG GACGGTGTTC 
CAAGCCAAGG AGATTCTGGA CAGCACCCTC GTGATTATCA AGAATGCCTT ATCAGCCACT 
GTGATAGACC CGGACCGGAT AGCGCTGGGA ACCGAGGAGG GGCTGTACTG CGTCGACCTG 
GACCGAGAAG AGATTGCTCG GATCGGGGAC GGCAAGAGGA TCGCTCAGCT CGAGTACATT 
CCTGATGAGC AGTTGCTCAT TGCCATAGCA GGCAAGCAGC GACACTTGCG GCTGATTCCC 
GTGGGGGCAC TGGACGGCCA CGAGGTCGAC TGGATCAAGG TGGCGGACAC CAAGGGCTGC 
TCGAGCTTCT GCACCACCCG CATGGACCAT GGGGGCTCGT CTACTTACTA CTTCTGTGTG 
GCCGTCAAGA AGCAGGTGCT GGTACACCAA GTGAACCGCA CCAAGAGCCG GCACGGGGGC 
CGGCAGGTGA TCAGCGTGCC CGGGGTGGCC CAGTGCCTGG ACGTCTGGCA AGACCGCCTC 
TGCGTGGGGC TGCCCTCCCT CTTCTACCTT TACAGCCTAC AGGGCACCAC CGAGCCCATC 
TGCCTGGTGC AGCCGGACAA CAACCTGTCC TTCCTGTACC ACAACCCGAT GGATGCCTTC 
ATGGCTGTGG AGCTCCCCAA CAACGAGTAC CTACTCGTCT TCAGCCAGCT TGGAGTGTTT 
GTGAACAACC TTGGGAAGAA GACCCGGGAG AAAGAAGTTA TGTTCTCGGC CTTACCGCTG 
GCAGTCAGTT ACCACGACTC CTACCTGTGC GTCTACATGG AGAGCCACGT GGATGTGTAC 
GAGGTGACCT CTGGGGACTG GGTGCAGACC ATGAACCTCA AGAAGACGAA GCCCCTGTGC 
CGGCAGGGCC AGCTGTGCTT CTCGCTGACG ACGGAGGCGC CCCAGGTGCT GTACCTGCAC 
CCCGTGCACC GGAGGGAGCA CGCCGTGAGG GTGGAGCCCC ACCGGCCAGG GGCCCCCCAC 
GCCCTGGGGC CCCTCAGGGG TTCCTCGTCC TCCGTCGCTG CCTCCCTGGT CACCAGGGCC 
CGCAGGAGGT TCTCCGTGCG CGAGCAGGAG AAGCTGCCCA AGGGGGACCG CCGGTCGAGG 
ATGATCTCTG CACCCTCGAA CTTCAACCAC ATCCAACACA TGGGACCCAG CGAGCTCCAG 
AAACTCATCG ACCTGCCCAC GACGGCACAG GGGGTTGCAG CCATGGAGGA GAAGAGGGCT 
TGGGTGAAGT CTGTGAAACC TCCAGGTCTG TTTCAAGCGT CACACCTCGA AAGCAGAACC 
CAAGGGGTGC CCCTGCAGCC CAAGCGGCCC GCCCCCCTGG CTCCCGGCCA CAGAACTGCC 
AAGTCGTCGC TGACAGCCTC TCCCGACGGA TCGCTCTCTT CTCAGGAGCA CACCTCCGGT 
CCGCAGGCCT CCTCGGCTCA CGACGACTCT GGGCACCAGT CGAGTCCGCG GCACTCCATC 
GCCTCCAACA ACAGCTCCAA CTTGAGCAGC CCCCCAAGTC CGGTGGATCT CGGCCTTGGC 
TTGGAAGATG CGCGCGAGCA GTTCTCCTCG AGCTACGAGT CGCAGAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029822570.1
CDS	650..5920
Translation	MSKKGDCRCPEERIRELEQMFLGGPVLASGKAFTVEGLIDVLVVLYDECCNSSLRKEKTMTNFIEYVKPVVQRIKDLRLTKDDFDVLKVIGRGAFGEVAVVRERNTGQVYAMKILNKWEMLKRAETACFQEERDVLVFGDRRWITNLHYSFQDETNLYLVMDYYCGGDLLTLLSKFEDKLPEEMSRFYITEMILAVDSIHSLHYVHRDIKPDNVLLDANGHIRLADFGSCLRLCDDGTVQSNVAVGTPDYISPEILRAMEDGQGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKNCFDFPDDPGCSVSEDAKDLMRRLICSADCRLGQNGLEDFKRHPWFKGVDWDHVRQSQAPYIPEVSSPTDTSNFDVDEADLKQPESGPPSANAVFSGLHLPFVGFTYTLGSRLAQGEETAVDDSHVVCRRKIQALEREKQDMCQRMARDLKRGLPNSGGGGDVVLRNSSTTDGEVRKLQDEVNTFRKKNAELQLELEQHQPLSNSHGGHSVGVGDVKVKELEKALRLARAEKDDIHRDLTEAQEKLKQQTKELKDALAQRKLAMDEYTEVSDKLSDLRTQKQKLSRQVRDKEEELEKAMHKIDSLRQDLRKADKLRRELEARAEETQAEALKERRLRERSDEYVHHLEGEVEALRQCQLGRLSTQGTDVSQEAARLKSELENQEVKFRELEIQLQTRHATELNSVRDQLREADSARDTLFKEVMMLKEKNEKYRAESAMESQETLAEIKKAHEREKAMLSEEVRKLTREGEQLAETVRRLHEERRCFEEEMGQLREKKESVAQWEAQISEIIQWVSDEKDARGYLQALATKMTEELDYLRMSGLPTPTAEKNWRNRRSQKLEKMELLTLQSSLQSEIQAKQVVCEELSRVRAELVAQQKEHRKIQQEFEHFRKESTKKDSQIRDLQQRLEATGDGFLERPSSQMSFLDQFLKDTSRLNHSESGESAGEAVANDTTESEMENQVPPPPIVRSSTSTEERRGIKISSPISDARPTITVLTPKPKAHQFLVRTFVAPLKCNHCTSLMVGLIRQGAVCEVCGFACHVTCQDKVPAVCPVPMDQSEQTFFQTAASKRPVGIDPTRGVGTAYEGYVKVPKLGGVKKGWQRQFVAVCDFKLFLYDLLQEKNVQPAVSASQVLDMRDEEFSVTSVLESDVIHANRKDIPCIFRVSTSMMDPPGLRSQTLMMVDKESEKVKWVDALNELHRILRRNKLPHRTVFQAKEILDSTLVIIKNALSATVIDPDRIALGTEEGLYCVDLDREEIARIGDGKRIAQLEYIPDEQLLIAIAGKQRHLRLIPVGALDGHEVDWIKVADTKGCSSFCTTRMDHGGSSTYYFCVAVKKQVLVHQVNRTKSRHGGRQVISVPGVAQCLDVWQDRLCVGLPSLFYLYSLQGTTEPICLVQPDNNLSFLYHNPMDAFMAVELPNNEYLLVFSQLGVFVNNLGKKTREKEVMFSALPLAVSYHDSYLCVYMESHVDVYEVTSGDWVQTMNLKKTKPLCRQGQLCFSLTTEAPQVLYLHPVHRREHAVRVEPHRPGAPHALGPLRGSSSSVAASLVTRARRRFSVREQEKLPKGDRRSRMISAPSNFNHIQHMGPSELQKLIDLPTTAQGVAAMEEKRAWVKSVKPPGLFQASHLESRTQGVPLQPKRPAPLAPGHRTAKSSLTASPDGSLSSQEHTSGPQASSAHDDSGHQSSPRHSIASNNSSNLSSPPSPVDLGLGLEDAREQFSSSYESQS

Target ORF information:

RefSeq Version	XM_029966710.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis serine/threonine-protein kinase MRCK alpha (LOC8026993), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029966710.1

ORF Insert Sequence:

ATGTCCAAGA AAGGGGACTG CCGCTGCCCC GAGGAGCGGA TACGCGAGCT CGAACAAATG 
TTCCTCGGAG GGCCCGTGCT GGCCAGCGGC AAGGCCTTCA CCGTCGAGGG CCTCATCGAC 
GTGCTGGTGG TGCTTTACGA CGAGTGCTGC AACTCGTCGC TACGCAAGGA GAAGACAATG 
ACCAACTTCA TCGAGTACGT CAAACCTGTG GTTCAGAGGA TCAAAGACCT GAGGCTGACC 
AAGGACGACT TCGACGTGCT CAAGGTGATA GGAAGGGGAG CGTTTGGAGA GGTGGCTGTG 
GTGCGAGAGC GGAACACGGG CCAAGTGTAC GCCATGAAGA TCCTCAACAA GTGGGAAATG 
CTCAAGAGGG CGGAGACGGC CTGTTTCCAA GAAGAGCGTG ACGTCCTCGT TTTCGGAGAC 
AGGCGGTGGA TCACGAATCT GCACTACTCC TTTCAAGATG AGACCAACTT GTACCTGGTA 
ATGGACTACT ACTGTGGCGG CGACCTGCTC ACGCTGCTGA GCAAGTTCGA GGACAAGCTG 
CCCGAGGAGA TGTCCCGCTT CTACATCACG GAGATGATCC TGGCCGTGGA CTCCATCCAC 
TCGCTCCACT ACGTCCACCG GGACATCAAG CCAGACAACG TGCTGCTGGA CGCCAACGGA 
CACATTCGCC TGGCCGACTT TGGCTCCTGC CTCAGGCTGT GCGACGACGG AACGGTTCAG 
TCCAACGTTG CGGTCGGCAC CCCTGACTAC ATATCTCCCG AAATCCTAAG GGCGATGGAA 
GATGGGCAGG GCAGGTACGG CCCGGAGTGC GACTGGTGGT CCCTGGGCGT CTGCATGTAC 
GAGATGCTGT ACGGGGAGAC GCCTTTCTAC GCCGAGTCCC TCGTGGAGAC CTACGGCAAG 
ATCATGAACC ACAAGAACTG CTTCGACTTC CCGGACGATC CGGGCTGCTC GGTGTCGGAG 
GATGCCAAGG ACCTGATGCG GCGGCTCATC TGCAGCGCGG ACTGCCGGCT GGGTCAGAAC 
GGCCTGGAGG ACTTCAAGCG CCACCCCTGG TTCAAAGGGG TCGACTGGGA CCACGTACGC 
CAGAGCCAGG CGCCCTACAT TCCGGAGGTG AGCAGTCCGA CGGACACCTC CAACTTCGAC 
GTGGACGAGG CCGACCTCAA GCAGCCCGAG TCCGGCCCCC CGAGCGCCAA TGCGGTGTTC 
TCTGGGCTGC ACCTGCCCTT TGTGGGCTTC ACGTACACCC TGGGCAGCCG GCTGGCCCAG 
GGGGAGGAGA CGGCCGTGGA CGACAGCCAC GTCGTCTGCC GCAGGAAGAT CCAGGCCCTG 
GAGCGGGAGA AGCAGGACAT GTGCCAGAGG ATGGCCAGGG ACCTCAAGAG GGGGCTCCCC 
AACTCTGGCG GCGGAGGTGA CGTGGTGCTG CGCAACAGCT CCACCACGGA CGGGGAGGTG 
CGCAAGCTGC AGGACGAGGT GAACACCTTC CGGAAGAAGA ATGCTGAACT GCAGCTAGAG 
CTGGAGCAGC ACCAGCCCCT GTCCAACTCG CACGGCGGCC ACTCCGTCGG GGTGGGCGAC 
GTCAAGGTCA AGGAGCTGGA GAAGGCGCTG CGTCTCGCAC GGGCGGAGAA GGATGACATC 
CACCGCGACC TGACAGAAGC CCAAGAGAAG CTGAAGCAGC AGACAAAGGA ACTGAAGGAT 
GCCCTAGCCC AGAGGAAACT AGCCATGGAT GAATACACCG AAGTCAGCGA CAAGCTGTCC 
GACCTGCGCA CCCAGAAGCA GAAGCTGTCG CGGCAGGTGC GGGACAAGGA GGAGGAACTG 
GAGAAGGCCA TGCACAAGAT CGACAGCCTC CGGCAAGACC TCAGGAAGGC CGACAAGCTG 
CGCAGAGAAC TGGAGGCCCG GGCGGAGGAG ACCCAGGCGG AAGCCCTGAA GGAGCGGCGC 
TTGAGAGAGC GCAGCGACGA GTACGTGCAC CACCTGGAGG GGGAAGTGGA GGCCCTGCGC 
CAATGCCAGC TGGGACGCCT CTCCACCCAG GGCACGGACG TCTCCCAGGA GGCCGCCAGG 
TTGAAGAGTG AGCTGGAGAA CCAAGAGGTG AAGTTCCGGG AGCTGGAGAT TCAGCTGCAG 
ACGAGGCACG CGACCGAGCT GAACTCGGTG CGTGACCAGC TCCGCGAAGC CGACAGCGCC 
CGGGACACCC TCTTCAAGGA GGTGATGATG CTCAAGGAGA AGAATGAAAA GTACCGGGCT 
GAAAGCGCCA TGGAGAGCCA GGAGACCCTG GCGGAGATCA AGAAGGCCCA CGAGCGGGAG 
AAGGCCATGC TCTCCGAAGA GGTCCGCAAG CTCACCCGGG AAGGGGAGCA GCTGGCGGAG 
ACTGTGAGGA GACTGCACGA GGAGCGGAGG TGCTTCGAAG AGGAGATGGG CCAGCTGCGG 
GAGAAGAAGG AGTCCGTCGC TCAGTGGGAG GCACAGATCA GCGAGATCAT CCAGTGGGTG 
AGCGACGAAA AGGATGCCCG TGGCTACCTG CAAGCCCTGG CCACTAAGAT GACGGAGGAG 
CTGGACTACC TGAGGATGAG CGGACTGCCC ACGCCCACTG CGGAGAAGAA CTGGCGGAAC 
CGGCGGTCCC AGAAGCTGGA AAAGATGGAG CTGCTCACCC TGCAGTCGAG CCTCCAGAGC 
GAGATCCAGG CCAAGCAGGT GGTCTGCGAG GAGCTGAGCC GGGTGCGGGC CGAGCTGGTC 
GCCCAGCAGA AGGAGCACCG CAAGATTCAG CAGGAGTTTG AGCACTTCCG GAAGGAGTCT 
ACCAAGAAGG ACTCCCAGAT CCGAGACCTG CAGCAGCGCT TGGAAGCTAC AGGTGACGGA 
TTCCTGGAGC GGCCGTCTTC GCAGATGTCT TTCCTCGACC AGTTCCTCAA GGACACCTCG 
CGGCTCAATC ACAGCGAAAG CGGGGAGTCC GCCGGAGAAG CCGTTGCCAA CGACACGACA 
GAGTCTGAGA TGGAGAACCA GGTGCCGCCG CCGCCCATCG TCAGGTCCAG CACCTCCACT 
GAGGAACGGC GAGGAATCAA AATCTCCTCC CCGATTAGCG ACGCGCGTCC CACAATCACG 
GTTCTCACGC CCAAGCCCAA GGCGCACCAG TTCCTGGTGC GGACGTTTGT GGCGCCCCTC 
AAGTGCAACC ACTGCACCTC CCTCATGGTG GGCCTCATCC GGCAAGGGGC TGTGTGCGAG 
GTGTGTGGCT TTGCCTGCCA CGTCACGTGT CAAGACAAGG TGCCTGCCGT GTGTCCAGTG 
CCAATGGATC AGAGTGAGCA GACCTTCTTT CAGACTGCCG CCTCAAAAAG GCCAGTTGGA 
ATCGACCCAA CAAGAGGCGT GGGAACAGCC TACGAAGGAT ATGTCAAGGT GCCCAAACTG 
GGCGGCGTGA AGAAAGGCTG GCAGAGGCAG TTTGTGGCTG TCTGCGACTT CAAGCTCTTC 
CTGTACGACC TGCTGCAGGA GAAGAACGTC CAGCCGGCGG TGTCTGCCTC CCAGGTGCTC 
GACATGAGGG ACGAGGAATT CTCGGTCACC TCCGTGCTCG AGTCGGACGT GATCCACGCC 
AACCGGAAGG ACATCCCGTG CATCTTTCGA GTGTCGACGT CCATGATGGA TCCCCCCGGG 
CTGCGCAGCC AGACGCTGAT GATGGTGGAC AAGGAGAGCG AGAAGGTCAA GTGGGTGGAC 
GCCCTCAATG AGCTGCACCG CATCCTCCGC CGGAACAAGC TGCCCCACAG GACGGTGTTC 
CAAGCCAAGG AGATTCTGGA CAGCACCCTC GTGATTATCA AGAATGCCTT ATCAGCCACT 
GTGATAGACC CGGACCGGAT AGCGCTGGGA ACCGAGGAGG GGCTGTACTG CGTCGACCTG 
GACCGAGAAG AGATTGCTCG GATCGGGGAC GGCAAGAGGA TCGCTCAGCT CGAGTACATT 
CCTGATGAGC AGTTGCTCAT TGCCATAGCA GGCAAGCAGC GACACTTGCG GCTGATTCCC 
GTGGGGGCAC TGGACGGCCA CGAGGTCGAC TGGATCAAGG TGGCGGACAC CAAGGGCTGC 
TCGAGCTTCT GCACCACCCG CATGGACCAT GGGGGCTCGT CTACTTACTA CTTCTGTGTG 
GCCGTCAAGA AGCAGGTGCT GGTACACCAA GTGAACCGCA CCAAGAGCCG GCACGGGGGC 
CGGCAGGTGA TCAGCGTGCC CGGGGTGGCC CAGTGCCTGG ACGTCTGGCA AGACCGCCTC 
TGCGTGGGGC TGCCCTCCCT CTTCTACCTT TACAGCCTAC AGGGCACCAC CGAGCCCATC 
TGCCTGGTGC AGCCGGACAA CAACCTGTCC TTCCTGTACC ACAACCCGAT GGATGCCTTC 
ATGGCTGTGG AGCTCCCCAA CAACGAGTAC CTACTCGTCT TCAGCCAGCT TGGAGTGTTT 
GTGAACAACC TTGGGAAGAA GACCCGGGAG AAAGAAGTTA TGTTCTCGGC CTTACCGCTG 
GCAGTCAGTT ACCACGACTC CTACCTGTGC GTCTACATGG AGAGCCACGT GGATGTGTAC 
GAGGTGACCT CTGGGGACTG GGTGCAGACC ATGAACCTCA AGAAGACGAA GCCCCTGTGC 
CGGCAGGGCC AGCTGTGCTT CTCGCTGACG ACGGAGGCGC CCCAGGTGCT GTACCTGCAC 
CCCGTGCACC GGAGGGAGCA CGCCGTGAGG GTGGAGCCCC ACCGGCCAGG GGCCCCCCAC 
GCCCTGGGGC CCCTCAGGGG TTCCTCGTCC TCCGTCGCTG CCTCCCTGGT CACCAGGGCC 
CGCAGGAGGT TCTCCGTGCG CGAGCAGGAG AAGCTGCCCA AGGGGGACCG CCGGTCGAGG 
ATGATCTCTG CACCCTCGAA CTTCAACCAC ATCCAACACA TGGGACCCAG CGAGCTCCAG 
AAACTCATCG ACCTGCCCAC GACGGCACAG GGGGTTGCAG CCATGGAGGA GAAGAGGGCT 
TGGGTGAAGT CTGTGAAACC TCCAGGTCTG TTTCAAGCGT CACACCTCGA AAGCAGAACC 
CAAGGGGTGC CCCTGCAGCC CAAGCGGCCC GCCCCCCTGG CTCCCGGCCA CAGAACTGCC 
AAGTCGTCGC TGACAGCCTC TCCCGACGGA TCGCTCTCTT CTCAGGAGCA CACCTCCGGT 
CCGCAGGCCT CCTCGGCTCA CGACGACTCT GGGCACCAGT CGAGTCCGCG GCACTCCATC 
GCCTCCAACA ACAGCTCCAA CTTGAGCAGC CCCCCAAGTC CGGTGGATCT CGGCCTTGGC 
TTGGAAGATG CGCGCGAGCA GTTCTCCTCG AGCTACGAGT CGCAGAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8026993 ( XM_029966711.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029822571.1 Ixodes scapularis serine/threonine-protein kinase MRCK alpha (LOC8026993), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OIa37170
Clone ID Related Accession (Same CDS sequence)	XM_029966711.1
Accession Version	XM_029966711.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	serine/threonine-protein kinase MRCK alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021922756.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGA AAGGGGACTG CCGCTGCCCC GAGGAGCGGA TACGCGAGCT CGAACAAATG 
TTCCTCGGAG GGCCCGTGCT GGCCAGCGGC AAGGCCTTCA CCGTCGAGGG CCTCATCGAC 
GTGCTGGTGG TGCTTTACGA CGAGTGCTGC AACTCGTCGC TACGCAAGGA GAAGACAATG 
ACCAACTTCA TCGAGTACGT CAAACCTGTG GTTCAGAGGA TCAAAGACCT GAGGCTGACC 
AAGGACGACT TCGACGTGCT CAAGGTGATA GGAAGGGGAG CGTTTGGAGA GGTGGCTGTG 
GTGCGAGAGC GGAACACGGG CCAAGTGTAC GCCATGAAGA TCCTCAACAA GTGGGAAATG 
CTCAAGAGGG CGGAGACGGC CTGTTTCCAA GAAGAGCGTG ACGTCCTCGT TTTCGGAGAC 
AGGCGGTGGA TCACGAATCT GCACTACTCC TTTCAAGATG AGACCAACTT GTACCTGGTA 
ATGGACTACT ACTGTGGCGG CGACCTGCTC ACGCTGCTGA GCAAGTTCGA GGACAAGCTG 
CCCGAGGAGA TGTCCCGCTT CTACATCACG GAGATGATCC TGGCCGTGGA CTCCATCCAC 
TCGCTCCACT ACGTCCACCG GGACATCAAG CCAGACAACG TGCTGCTGGA CGCCAACGGA 
CACATTCGCC TGGCCGACTT TGGCTCCTGC CTCAGGCTGT GCGACGACGG AACGGTTCAG 
TCCAACGTTG CGGTCGGCAC CCCTGACTAC ATATCTCCCG AAATCCTAAG GGCGATGGAA 
GATGGGCAGG GCAGGTACGG CCCGGAGTGC GACTGGTGGT CCCTGGGCGT CTGCATGTAC 
GAGATGCTGT ACGGGGAGAC GCCTTTCTAC GCCGAGTCCC TCGTGGAGAC CTACGGCAAG 
ATCATGAACC ACAAGAACTG CTTCGACTTC CCGGACGATC CGGGCTGCTC GGTGTCGGAG 
GATGCCAAGG ACCTGATGCG GCGGCTCATC TGCAGCGCGG ACTGCCGGCT GGGTCAGAAC 
GGCCTGGAGG ACTTCAAGCG CCACCCCTGG TTCAAAGGGG TCGACTGGGA CCACGTACGC 
CAGAGCCAGG CGCCCTACAT TCCGGAGGTG AGCAGTCCGA CGGACACCTC CAACTTCGAC 
GTGGACGAGG CCGACCTCAA GCAGCCCGAG TCCGGCCCCC CGAGCGCCAA TGCGGTGTTC 
TCTGGGCTGC ACCTGCCCTT TGTGGGCTTC ACGTACACCC TGGGCAGCCG GCTGGCCCAG 
GGGGAGGAGA CGGCCGTGGA CGACAGCCAC GTCGTCTGCC GCAGGAAGAT CCAGGCCCTG 
GAGCGGGAGA AGCAGGACAT GTGCCAGAGG ATGGCCAGGG ACCTCAAGAG GGGGCTCCCC 
AACTCTGGCG GCGGAGGTGA CGTGGTGCTG CGCAACAGCT CCACCACGGA CGGGGAGGTG 
CGCAAGCTGC AGGACGAGGT GAACACCTTC CGGAAGAAGA ATGCTGAACT GCAGCTAGAG 
CTGGAGCAGC ACCAGCCCCT GTCCAACTCG CACGGCGGCC ACTCCGTCGG GGTGGGCGAC 
GTCAAGGTCA AGGAGCTGGA GAAGGCGCTG CGTCTCGCAC GGGCGGAGAA GGATGACATC 
CACCGCGACC TGACAGAAGC CCAAGAGAAG CTGAAGCAGC AGACAAAGGA ACTGAAGGAT 
GCCCTAGCCC AGAGGAAACT AGCCATGGAT GAATACACCG AAGTCAGCGA CAAGCTGTCC 
GACCTGCGCA CCCAGAAGCA GAAGCTGTCG CGGCAGGTGC GGGACAAGGA GGAGGAACTG 
GAGAAGGCCA TGCACAAGAT CGACAGCCTC CGGCAAGACC TCAGGAAGGC CGACAAGCTG 
CGCAGAGAAC TGGAGGCCCG GGCGGAGGAG ACCCAGGCGG AAGCCCTGAA GGAGCGGCGC 
TTGAGAGAGC GCAGCGACGA GTACGTGCAC CACCTGGAGG GGGAAGTGGA GGCCCTGCGC 
CAATGCCAGC TGGGACGCCT CTCCACCCAG GGCACGGACG TCTCCCAGGA GGCCGCCAGG 
TTGAAGAGTG AGCTGGAGAA CCAAGAGGTG AAGTTCCGGG AGCTGGAGAT TCAGCTGCAG 
ACGAGGCACG CGACCGAGCT GAACTCGGTG CGTGACCAGC TCCGCGAAGC CGACAGCGCC 
CGGGACACCC TCTTCAAGGA GGTGATGATG CTCAAGGAGA AGAATGAAAA GTACCGGGCT 
GAAAGCGCCA TGGAGAGCCA GGAGACCCTG GCGGAGATCA AGAAGGCCCA CGAGCGGGAG 
AAGGCCATGC TCTCCGAAGA GGTCCGCAAG CTCACCCGGG AAGGGGAGCA GCTGGCGGAG 
ACTGTGAGGA GACTGCACGA GGAGCGGAGG TGCTTCGAAG AGGAGATGGG CCAGCTGCGG 
GAGAAGAAGG AGTCCGTCGC TCAGTGGGAG GCACAGATCA GCGAGATCAT CCAGTGGGTG 
AGCGACGAAA AGGATGCCCG TGGCTACCTG CAAGCCCTGG CCACTAAGAT GACGGAGGAG 
CTGGACTACC TGAGGATGAG CGGACTGCCC ACGCCCACTG CGGAGAAGAA CTGGCGGAAC 
CGGCGGTCCC AGAAGCTGGA AAAGATGGAG CTGCTCACCC TGCAGTCGAG CCTCCAGAGC 
GAGATCCAGG CCAAGCAGGT GGTCTGCGAG GAGCTGAGCC GGGTGCGGGC CGAGCTGGTC 
GCCCAGCAGA AGGAGCACCG CAAGATTCAG CAGGAGTTTG AGCACTTCCG GAAGGAGTCT 
ACCAAGAAGG ACTCCCAGAT CCGAGACCTG CAGCAGCGCT TGGAAGCTAC AGGTGACGGA 
TTCCTGGAGC GGCCGTCTTC GCAGATGTCT TTCCTCGACC AGTTCCTCAA GGACACCTCG 
CGGCTCAATC ACAGCGAAAG CGGGGAGTCC GCCGGAGAAG CCGTTGCCAA CGACACGACA 
GAGTCTGAGA TGGAGAACCA GGTGCCGCCG CCGCCCATCG TCAGGTCCAG CACCTCCACT 
GAGGAACGGC GAGGAATCAA AATCTCCTCC CCGATTAGCG ACGCGCGTCC CACAATCACG 
GTTCTCACGC CCAAGCCCAA GGCGCACCAG TTCCTGGTGC GGACGTTTGT GGCGCCCCTC 
AAGTGCAACC ACTGCACCTC CCTCATGGTG GGCCTCATCC GGCAAGGGGC TGTGTGCGAG 
GTGTGTGGCT TTGCCTGCCA CGTCACGTGT CAAGACAAGG TGCCTGCCGT GTGTCCAGTG 
CCAATGGATC AGACAAAAAG GCCAGTTGGA ATCGACCCAA CAAGAGGCGT GGGAACAGCC 
TACGAAGGAT ATGTCAAGGT GCCCAAACTG GGCGGCGTGA AGAAAGGCTG GCAGAGGCAG 
TTTGTGGCTG TCTGCGACTT CAAGCTCTTC CTGTACGACC TGCTGCAGGA GAAGAACGTC 
CAGCCGGCGG TGTCTGCCTC CCAGGTGCTC GACATGAGGG ACGAGGAATT CTCGGTCACC 
TCCGTGCTCG AGTCGGACGT GATCCACGCC AACCGGAAGG ACATCCCGTG CATCTTTCGA 
GTGTCGACGT CCATGATGGA TCCCCCCGGG CTGCGCAGCC AGACGCTGAT GATGGTGGAC 
AAGGAGAGCG AGAAGGTCAA GTGGGTGGAC GCCCTCAATG AGCTGCACCG CATCCTCCGC 
CGGAACAAGC TGCCCCACAG GACGGTGTTC CAAGCCAAGG AGATTCTGGA CAGCACCCTC 
GTGATTATCA AGAATGCCTT ATCAGCCACT GTGATAGACC CGGACCGGAT AGCGCTGGGA 
ACCGAGGAGG GGCTGTACTG CGTCGACCTG GACCGAGAAG GTGAGGGAGA GATTGCTCGG 
ATCGGGGACG GCAAGAGGAT CGCTCAGCTC GAGTACATTC CTGATGAGCA GTTGCTCATT 
GCCATAGCAG GCAAGCAGCG ACACTTGCGG CTGATTCCCG TGGGGGCACT GGACGGCCAC 
GAGGTCGACT GGATCAAGGT GGCGGACACC AAGGGCTGCT CGAGCTTCTG CACCACCCGC 
ATGGACCATG GGGGCTCGTC TACTTACTAC TTCTGTGTGG CCGTCAAGAA GCAGGTGCTG 
GTACACCAAG TGAACCGCAC CAAGAGCCGG CACGGGGGCC GGCAGGTGAT CAGCGTGCCC 
GGGGTGGCCC AGTGCCTGGA CGTCTGGCAA GACCGCCTCT GCGTGGGGCT GCCCTCCCTC 
TTCTACCTTT ACAGCCTACA GGGCACCACC GAGCCCATCT GCCTGGTGCA GCCGGACAAC 
AACCTGTCCT TCCTGTACCA CAACCCGATG GATGCCTTCA TGGCTGTGGA GCTCCCCAAC 
AACGAGTACC TACTCGTCTT CAGCCAGCTT GGAGTGTTTG TGAACAACCT TGGGAAGAAG 
ACCCGGGAGA AAGAAGTTAT GTTCTCGGCC TTACCGCTGG CAGTCAGTTA CCACGACTCC 
TACCTGTGCG TCTACATGGA GAGCCACGTG GATGTGTACG AGGTGACCTC TGGGGACTGG 
GTGCAGACCA TGAACCTCAA GAAGACGAAG CCCCTGTGCC GGCAGGGCCA GCTGTGCTTC 
TCGCTGACGA CGGAGGCGCC CCAGGTGCTG TACCTGCACC CCGTGCACCG GAGGGAGCAC 
GCCGTGAGGG TGGAGCCCCA CCGGCCAGGG GCCCCCCACG CCCTGGGGCC CCTCAGGGGT 
TCCTCGTCCT CCGTCGCTGC CTCCCTGGTC ACCAGGGCCC GCAGGAGGTT CTCCGTGCGC 
GAGCAGGAGA AGCTGCCCAA GGGGGACCGC CGGTCGAGGA TGATCTCTGC ACCCTCGAAC 
TTCAACCACA TCCAACACAT GGGACCCAGC GAGCTCCAGA AACTCATCGA CCTGCCCACG 
ACGGCACAGG GGGTTGCAGC CATGGAGGAG AAGAGGGCTT GGGTGAAGTC TGTGAAACCT 
CCAGGTCTGT TTCAAGCGTC ACACCTCGAA AGCAGAACCC AAGGGGTGCC CCTGCAGCCC 
AAGCGGCCCG CCCCCCTGGC TCCCGGCCAC AGAACTGCCA AGTCGTCGCT GACAGCCTCT 
CCCGACGGAT CGCTCTCTTC TCAGGAGCAC ACCTCCGGTC CGCAGGCCTC CTCGGCTCAC 
GACGACTCTG GGCACCAGTC GAGTCCGCGG CACTCCATCG CCTCCAACAA CAGCTCCAAC 
TTGAGCAGCC CCCCAAGTCC GGTGGATCTC GGCCTTGGCT TGGAAGATGC GCGCGAGCAG 
TTCTCCTCGA GCTACGAGTC GCAGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029822571.1
CDS	650..5899
Translation	MSKKGDCRCPEERIRELEQMFLGGPVLASGKAFTVEGLIDVLVVLYDECCNSSLRKEKTMTNFIEYVKPVVQRIKDLRLTKDDFDVLKVIGRGAFGEVAVVRERNTGQVYAMKILNKWEMLKRAETACFQEERDVLVFGDRRWITNLHYSFQDETNLYLVMDYYCGGDLLTLLSKFEDKLPEEMSRFYITEMILAVDSIHSLHYVHRDIKPDNVLLDANGHIRLADFGSCLRLCDDGTVQSNVAVGTPDYISPEILRAMEDGQGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKNCFDFPDDPGCSVSEDAKDLMRRLICSADCRLGQNGLEDFKRHPWFKGVDWDHVRQSQAPYIPEVSSPTDTSNFDVDEADLKQPESGPPSANAVFSGLHLPFVGFTYTLGSRLAQGEETAVDDSHVVCRRKIQALEREKQDMCQRMARDLKRGLPNSGGGGDVVLRNSSTTDGEVRKLQDEVNTFRKKNAELQLELEQHQPLSNSHGGHSVGVGDVKVKELEKALRLARAEKDDIHRDLTEAQEKLKQQTKELKDALAQRKLAMDEYTEVSDKLSDLRTQKQKLSRQVRDKEEELEKAMHKIDSLRQDLRKADKLRRELEARAEETQAEALKERRLRERSDEYVHHLEGEVEALRQCQLGRLSTQGTDVSQEAARLKSELENQEVKFRELEIQLQTRHATELNSVRDQLREADSARDTLFKEVMMLKEKNEKYRAESAMESQETLAEIKKAHEREKAMLSEEVRKLTREGEQLAETVRRLHEERRCFEEEMGQLREKKESVAQWEAQISEIIQWVSDEKDARGYLQALATKMTEELDYLRMSGLPTPTAEKNWRNRRSQKLEKMELLTLQSSLQSEIQAKQVVCEELSRVRAELVAQQKEHRKIQQEFEHFRKESTKKDSQIRDLQQRLEATGDGFLERPSSQMSFLDQFLKDTSRLNHSESGESAGEAVANDTTESEMENQVPPPPIVRSSTSTEERRGIKISSPISDARPTITVLTPKPKAHQFLVRTFVAPLKCNHCTSLMVGLIRQGAVCEVCGFACHVTCQDKVPAVCPVPMDQTKRPVGIDPTRGVGTAYEGYVKVPKLGGVKKGWQRQFVAVCDFKLFLYDLLQEKNVQPAVSASQVLDMRDEEFSVTSVLESDVIHANRKDIPCIFRVSTSMMDPPGLRSQTLMMVDKESEKVKWVDALNELHRILRRNKLPHRTVFQAKEILDSTLVIIKNALSATVIDPDRIALGTEEGLYCVDLDREGEGEIARIGDGKRIAQLEYIPDEQLLIAIAGKQRHLRLIPVGALDGHEVDWIKVADTKGCSSFCTTRMDHGGSSTYYFCVAVKKQVLVHQVNRTKSRHGGRQVISVPGVAQCLDVWQDRLCVGLPSLFYLYSLQGTTEPICLVQPDNNLSFLYHNPMDAFMAVELPNNEYLLVFSQLGVFVNNLGKKTREKEVMFSALPLAVSYHDSYLCVYMESHVDVYEVTSGDWVQTMNLKKTKPLCRQGQLCFSLTTEAPQVLYLHPVHRREHAVRVEPHRPGAPHALGPLRGSSSSVAASLVTRARRRFSVREQEKLPKGDRRSRMISAPSNFNHIQHMGPSELQKLIDLPTTAQGVAAMEEKRAWVKSVKPPGLFQASHLESRTQGVPLQPKRPAPLAPGHRTAKSSLTASPDGSLSSQEHTSGPQASSAHDDSGHQSSPRHSIASNNSSNLSSPPSPVDLGLGLEDAREQFSSSYESQS

Target ORF information:

RefSeq Version	XM_029966711.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis serine/threonine-protein kinase MRCK alpha (LOC8026993), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029966711.1

ORF Insert Sequence:

ATGTCCAAGA AAGGGGACTG CCGCTGCCCC GAGGAGCGGA TACGCGAGCT CGAACAAATG 
TTCCTCGGAG GGCCCGTGCT GGCCAGCGGC AAGGCCTTCA CCGTCGAGGG CCTCATCGAC 
GTGCTGGTGG TGCTTTACGA CGAGTGCTGC AACTCGTCGC TACGCAAGGA GAAGACAATG 
ACCAACTTCA TCGAGTACGT CAAACCTGTG GTTCAGAGGA TCAAAGACCT GAGGCTGACC 
AAGGACGACT TCGACGTGCT CAAGGTGATA GGAAGGGGAG CGTTTGGAGA GGTGGCTGTG 
GTGCGAGAGC GGAACACGGG CCAAGTGTAC GCCATGAAGA TCCTCAACAA GTGGGAAATG 
CTCAAGAGGG CGGAGACGGC CTGTTTCCAA GAAGAGCGTG ACGTCCTCGT TTTCGGAGAC 
AGGCGGTGGA TCACGAATCT GCACTACTCC TTTCAAGATG AGACCAACTT GTACCTGGTA 
ATGGACTACT ACTGTGGCGG CGACCTGCTC ACGCTGCTGA GCAAGTTCGA GGACAAGCTG 
CCCGAGGAGA TGTCCCGCTT CTACATCACG GAGATGATCC TGGCCGTGGA CTCCATCCAC 
TCGCTCCACT ACGTCCACCG GGACATCAAG CCAGACAACG TGCTGCTGGA CGCCAACGGA 
CACATTCGCC TGGCCGACTT TGGCTCCTGC CTCAGGCTGT GCGACGACGG AACGGTTCAG 
TCCAACGTTG CGGTCGGCAC CCCTGACTAC ATATCTCCCG AAATCCTAAG GGCGATGGAA 
GATGGGCAGG GCAGGTACGG CCCGGAGTGC GACTGGTGGT CCCTGGGCGT CTGCATGTAC 
GAGATGCTGT ACGGGGAGAC GCCTTTCTAC GCCGAGTCCC TCGTGGAGAC CTACGGCAAG 
ATCATGAACC ACAAGAACTG CTTCGACTTC CCGGACGATC CGGGCTGCTC GGTGTCGGAG 
GATGCCAAGG ACCTGATGCG GCGGCTCATC TGCAGCGCGG ACTGCCGGCT GGGTCAGAAC 
GGCCTGGAGG ACTTCAAGCG CCACCCCTGG TTCAAAGGGG TCGACTGGGA CCACGTACGC 
CAGAGCCAGG CGCCCTACAT TCCGGAGGTG AGCAGTCCGA CGGACACCTC CAACTTCGAC 
GTGGACGAGG CCGACCTCAA GCAGCCCGAG TCCGGCCCCC CGAGCGCCAA TGCGGTGTTC 
TCTGGGCTGC ACCTGCCCTT TGTGGGCTTC ACGTACACCC TGGGCAGCCG GCTGGCCCAG 
GGGGAGGAGA CGGCCGTGGA CGACAGCCAC GTCGTCTGCC GCAGGAAGAT CCAGGCCCTG 
GAGCGGGAGA AGCAGGACAT GTGCCAGAGG ATGGCCAGGG ACCTCAAGAG GGGGCTCCCC 
AACTCTGGCG GCGGAGGTGA CGTGGTGCTG CGCAACAGCT CCACCACGGA CGGGGAGGTG 
CGCAAGCTGC AGGACGAGGT GAACACCTTC CGGAAGAAGA ATGCTGAACT GCAGCTAGAG 
CTGGAGCAGC ACCAGCCCCT GTCCAACTCG CACGGCGGCC ACTCCGTCGG GGTGGGCGAC 
GTCAAGGTCA AGGAGCTGGA GAAGGCGCTG CGTCTCGCAC GGGCGGAGAA GGATGACATC 
CACCGCGACC TGACAGAAGC CCAAGAGAAG CTGAAGCAGC AGACAAAGGA ACTGAAGGAT 
GCCCTAGCCC AGAGGAAACT AGCCATGGAT GAATACACCG AAGTCAGCGA CAAGCTGTCC 
GACCTGCGCA CCCAGAAGCA GAAGCTGTCG CGGCAGGTGC GGGACAAGGA GGAGGAACTG 
GAGAAGGCCA TGCACAAGAT CGACAGCCTC CGGCAAGACC TCAGGAAGGC CGACAAGCTG 
CGCAGAGAAC TGGAGGCCCG GGCGGAGGAG ACCCAGGCGG AAGCCCTGAA GGAGCGGCGC 
TTGAGAGAGC GCAGCGACGA GTACGTGCAC CACCTGGAGG GGGAAGTGGA GGCCCTGCGC 
CAATGCCAGC TGGGACGCCT CTCCACCCAG GGCACGGACG TCTCCCAGGA GGCCGCCAGG 
TTGAAGAGTG AGCTGGAGAA CCAAGAGGTG AAGTTCCGGG AGCTGGAGAT TCAGCTGCAG 
ACGAGGCACG CGACCGAGCT GAACTCGGTG CGTGACCAGC TCCGCGAAGC CGACAGCGCC 
CGGGACACCC TCTTCAAGGA GGTGATGATG CTCAAGGAGA AGAATGAAAA GTACCGGGCT 
GAAAGCGCCA TGGAGAGCCA GGAGACCCTG GCGGAGATCA AGAAGGCCCA CGAGCGGGAG 
AAGGCCATGC TCTCCGAAGA GGTCCGCAAG CTCACCCGGG AAGGGGAGCA GCTGGCGGAG 
ACTGTGAGGA GACTGCACGA GGAGCGGAGG TGCTTCGAAG AGGAGATGGG CCAGCTGCGG 
GAGAAGAAGG AGTCCGTCGC TCAGTGGGAG GCACAGATCA GCGAGATCAT CCAGTGGGTG 
AGCGACGAAA AGGATGCCCG TGGCTACCTG CAAGCCCTGG CCACTAAGAT GACGGAGGAG 
CTGGACTACC TGAGGATGAG CGGACTGCCC ACGCCCACTG CGGAGAAGAA CTGGCGGAAC 
CGGCGGTCCC AGAAGCTGGA AAAGATGGAG CTGCTCACCC TGCAGTCGAG CCTCCAGAGC 
GAGATCCAGG CCAAGCAGGT GGTCTGCGAG GAGCTGAGCC GGGTGCGGGC CGAGCTGGTC 
GCCCAGCAGA AGGAGCACCG CAAGATTCAG CAGGAGTTTG AGCACTTCCG GAAGGAGTCT 
ACCAAGAAGG ACTCCCAGAT CCGAGACCTG CAGCAGCGCT TGGAAGCTAC AGGTGACGGA 
TTCCTGGAGC GGCCGTCTTC GCAGATGTCT TTCCTCGACC AGTTCCTCAA GGACACCTCG 
CGGCTCAATC ACAGCGAAAG CGGGGAGTCC GCCGGAGAAG CCGTTGCCAA CGACACGACA 
GAGTCTGAGA TGGAGAACCA GGTGCCGCCG CCGCCCATCG TCAGGTCCAG CACCTCCACT 
GAGGAACGGC GAGGAATCAA AATCTCCTCC CCGATTAGCG ACGCGCGTCC CACAATCACG 
GTTCTCACGC CCAAGCCCAA GGCGCACCAG TTCCTGGTGC GGACGTTTGT GGCGCCCCTC 
AAGTGCAACC ACTGCACCTC CCTCATGGTG GGCCTCATCC GGCAAGGGGC TGTGTGCGAG 
GTGTGTGGCT TTGCCTGCCA CGTCACGTGT CAAGACAAGG TGCCTGCCGT GTGTCCAGTG 
CCAATGGATC AGACAAAAAG GCCAGTTGGA ATCGACCCAA CAAGAGGCGT GGGAACAGCC 
TACGAAGGAT ATGTCAAGGT GCCCAAACTG GGCGGCGTGA AGAAAGGCTG GCAGAGGCAG 
TTTGTGGCTG TCTGCGACTT CAAGCTCTTC CTGTACGACC TGCTGCAGGA GAAGAACGTC 
CAGCCGGCGG TGTCTGCCTC CCAGGTGCTC GACATGAGGG ACGAGGAATT CTCGGTCACC 
TCCGTGCTCG AGTCGGACGT GATCCACGCC AACCGGAAGG ACATCCCGTG CATCTTTCGA 
GTGTCGACGT CCATGATGGA TCCCCCCGGG CTGCGCAGCC AGACGCTGAT GATGGTGGAC 
AAGGAGAGCG AGAAGGTCAA GTGGGTGGAC GCCCTCAATG AGCTGCACCG CATCCTCCGC 
CGGAACAAGC TGCCCCACAG GACGGTGTTC CAAGCCAAGG AGATTCTGGA CAGCACCCTC 
GTGATTATCA AGAATGCCTT ATCAGCCACT GTGATAGACC CGGACCGGAT AGCGCTGGGA 
ACCGAGGAGG GGCTGTACTG CGTCGACCTG GACCGAGAAG GTGAGGGAGA GATTGCTCGG 
ATCGGGGACG GCAAGAGGAT CGCTCAGCTC GAGTACATTC CTGATGAGCA GTTGCTCATT 
GCCATAGCAG GCAAGCAGCG ACACTTGCGG CTGATTCCCG TGGGGGCACT GGACGGCCAC 
GAGGTCGACT GGATCAAGGT GGCGGACACC AAGGGCTGCT CGAGCTTCTG CACCACCCGC 
ATGGACCATG GGGGCTCGTC TACTTACTAC TTCTGTGTGG CCGTCAAGAA GCAGGTGCTG 
GTACACCAAG TGAACCGCAC CAAGAGCCGG CACGGGGGCC GGCAGGTGAT CAGCGTGCCC 
GGGGTGGCCC AGTGCCTGGA CGTCTGGCAA GACCGCCTCT GCGTGGGGCT GCCCTCCCTC 
TTCTACCTTT ACAGCCTACA GGGCACCACC GAGCCCATCT GCCTGGTGCA GCCGGACAAC 
AACCTGTCCT TCCTGTACCA CAACCCGATG GATGCCTTCA TGGCTGTGGA GCTCCCCAAC 
AACGAGTACC TACTCGTCTT CAGCCAGCTT GGAGTGTTTG TGAACAACCT TGGGAAGAAG 
ACCCGGGAGA AAGAAGTTAT GTTCTCGGCC TTACCGCTGG CAGTCAGTTA CCACGACTCC 
TACCTGTGCG TCTACATGGA GAGCCACGTG GATGTGTACG AGGTGACCTC TGGGGACTGG 
GTGCAGACCA TGAACCTCAA GAAGACGAAG CCCCTGTGCC GGCAGGGCCA GCTGTGCTTC 
TCGCTGACGA CGGAGGCGCC CCAGGTGCTG TACCTGCACC CCGTGCACCG GAGGGAGCAC 
GCCGTGAGGG TGGAGCCCCA CCGGCCAGGG GCCCCCCACG CCCTGGGGCC CCTCAGGGGT 
TCCTCGTCCT CCGTCGCTGC CTCCCTGGTC ACCAGGGCCC GCAGGAGGTT CTCCGTGCGC 
GAGCAGGAGA AGCTGCCCAA GGGGGACCGC CGGTCGAGGA TGATCTCTGC ACCCTCGAAC 
TTCAACCACA TCCAACACAT GGGACCCAGC GAGCTCCAGA AACTCATCGA CCTGCCCACG 
ACGGCACAGG GGGTTGCAGC CATGGAGGAG AAGAGGGCTT GGGTGAAGTC TGTGAAACCT 
CCAGGTCTGT TTCAAGCGTC ACACCTCGAA AGCAGAACCC AAGGGGTGCC CCTGCAGCCC 
AAGCGGCCCG CCCCCCTGGC TCCCGGCCAC AGAACTGCCA AGTCGTCGCT GACAGCCTCT 
CCCGACGGAT CGCTCTCTTC TCAGGAGCAC ACCTCCGGTC CGCAGGCCTC CTCGGCTCAC 
GACGACTCTG GGCACCAGTC GAGTCCGCGG CACTCCATCG CCTCCAACAA CAGCTCCAAC 
TTGAGCAGCC CCCCAAGTCC GGTGGATCTC GGCCTTGGCT TGGAAGATGC GCGCGAGCAG 
TTCTCCTCGA GCTACGAGTC GCAGAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8026993 ( XM_029966712.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029822572.1 Ixodes scapularis serine/threonine-protein kinase MRCK alpha (LOC8026993), transcript variant X4, mRNA.

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CloneID	OIa37171
Clone ID Related Accession (Same CDS sequence)	XM_029966712.1
Accession Version	XM_029966712.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	serine/threonine-protein kinase MRCK alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021922756.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGA AAGGGGACTG CCGCTGCCCC GAGGAGCGGA TACGCGAGCT CGAACAAATG 
TTCCTCGGAG GGCCCGTGCT GGCCAGCGGC AAGGCCTTCA CCGTCGAGGG CCTCATCGAC 
GTGCTGGTGG TGCTTTACGA CGAGTGCTGC AACTCGTCGC TACGCAAGGA GAAGACAATG 
ACCAACTTCA TCGAGTACGT CAAACCTGTG GTTCAGAGGA TCAAAGACCT GAGGCTGACC 
AAGGACGACT TCGACGTGCT CAAGGTGATA GGAAGGGGAG CGTTTGGAGA GGTGGCTGTG 
GTGCGAGAGC GGAACACGGG CCAAGTGTAC GCCATGAAGA TCCTCAACAA GTGGGAAATG 
CTCAAGAGGG CGGAGACGGC CTGTTTCCAA GAAGAGCGTG ACGTCCTCGT TTTCGGAGAC 
AGGCGGTGGA TCACGAATCT GCACTACTCC TTTCAAGATG AGACCAACTT GTACCTGGTA 
ATGGACTACT ACTGTGGCGG CGACCTGCTC ACGCTGCTGA GCAAGTTCGA GGACAAGCTG 
CCCGAGGAGA TGTCCCGCTT CTACATCACG GAGATGATCC TGGCCGTGGA CTCCATCCAC 
TCGCTCCACT ACGTCCACCG GGACATCAAG CCAGACAACG TGCTGCTGGA CGCCAACGGA 
CACATTCGCC TGGCCGACTT TGGCTCCTGC CTCAGGCTGT GCGACGACGG AACGGTTCAG 
TCCAACGTTG CGGTCGGCAC CCCTGACTAC ATATCTCCCG AAATCCTAAG GGCGATGGAA 
GATGGGCAGG GCAGGTACGG CCCGGAGTGC GACTGGTGGT CCCTGGGCGT CTGCATGTAC 
GAGATGCTGT ACGGGGAGAC GCCTTTCTAC GCCGAGTCCC TCGTGGAGAC CTACGGCAAG 
ATCATGAACC ACAAGAACTG CTTCGACTTC CCGGACGATC CGGGCTGCTC GGTGTCGGAG 
GATGCCAAGG ACCTGATGCG GCGGCTCATC TGCAGCGCGG ACTGCCGGCT GGGTCAGAAC 
GGCCTGGAGG ACTTCAAGCG CCACCCCTGG TTCAAAGGGG TCGACTGGGA CCACGTACGC 
CAGAGCCAGG CGCCCTACAT TCCGGAGGTG AGCAGTCCGA CGGACACCTC CAACTTCGAC 
GTGGACGAGG CCGACCTCAA GCAGCCCGAG TCCGGCCCCC CGAGCGCCAA TGCGGTGTTC 
TCTGGGCTGC ACCTGCCCTT TGTGGGCTTC ACGTACACCC TGGGCAGCCG GCTGGCCCAG 
GGGGAGGAGA CGGCCGTGGA CGACAGCCAC GTCGTCTGCC GCAGGAAGAT CCAGGCCCTG 
GAGCGGGAGA AGCAGGACAT GTGCCAGAGG ATGGCCAGGG ACCTCAAGAG GGGGCTCCCC 
AACTCTGGCG GCGGAGGTGA CGTGGTGCTG CGCAACAGCT CCACCACGGA CGGGGAGGTG 
CGCAAGCTGC AGGACGAGGT GAACACCTTC CGGAAGAAGA ATGCTGAACT GCAGCTAGAG 
CTGGAGCAGC ACCAGCCCCT GTCCAACTCG CACGGCGGCC ACTCCGTCGG GGTGGGCGAC 
GTCAAGGTCA AGGAGCTGGA GAAGGCGCTG CGTCTCGCAC GGGCGGAGAA GGATGACATC 
CACCGCGACC TGACAGAAGC CCAAGAGAAG CTGAAGCAGC AGACAAAGGA ACTGAAGGAT 
GCCCTAGCCC AGAGGAAACT AGCCATGGAT GAATACACCG AAGTCAGCGA CAAGCTGTCC 
GACCTGCGCA CCCAGAAGCA GAAGCTGTCG CGGCAGGTGC GGGACAAGGA GGAGGAACTG 
GAGAAGGCCA TGCACAAGAT CGACAGCCTC CGGCAAGACC TCAGGAAGGC CGACAAGCTG 
CGCAGAGAAC TGGAGGCCCG GGCGGAGGAG ACCCAGGCGG AAGCCCTGAA GGAGCGGCGC 
TTGAGAGAGC GCAGCGACGA GTACGTGCAC CACCTGGAGG GGGAAGTGGA GGCCCTGCGC 
CAATGCCAGC TGGGACGCCT CTCCACCCAG GGCACGGACG TCTCCCAGGA GGCCGCCAGG 
TTGAAGAGTG AGCTGGAGAA CCAAGAGGTG AAGTTCCGGG AGCTGGAGAT TCAGCTGCAG 
ACGAGGCACG CGACCGAGCT GAACTCGGTG CGTGACCAGC TCCGCGAAGC CGACAGCGCC 
CGGGACACCC TCTTCAAGGA GGTGATGATG CTCAAGGAGA AGAATGAAAA GTACCGGGCT 
GAAAGCGCCA TGGAGAGCCA GGAGACCCTG GCGGAGATCA AGAAGGCCCA CGAGCGGGAG 
AAGGCCATGC TCTCCGAAGA GGTCCGCAAG CTCACCCGGG AAGGGGAGCA GCTGGCGGAG 
ACTGTGAGGA GACTGCACGA GGAGCGGAGG TGCTTCGAAG AGGAGATGGG CCAGCTGCGG 
GAGAAGAAGG AGTCCGTCGC TCAGTGGGAG GCACAGATCA GCGAGATCAT CCAGTGGGTG 
AGCGACGAAA AGGATGCCCG TGGCTACCTG CAAGCCCTGG CCACTAAGAT GACGGAGGAG 
CTGGACTACC TGAGGATGAG CGGACTGCCC ACGCCCACTG CGGAGAAGAA CTGGCGGAAC 
CGGCGGTCCC AGAAGCTGGA AAAGATGGAG CTGCTCACCC TGCAGTCGAG CCTCCAGAGC 
GAGATCCAGG CCAAGCAGGT GGTCTGCGAG GAGCTGAGCC GGGTGCGGGC CGAGCTGGTC 
GCCCAGCAGA AGGAGCACCG CAAGATTCAG CAGGAGTTTG AGCACTTCCG GAAGGAGTCT 
ACCAAGAAGG ACTCCCAGAT CCGAGACCTG CAGCAGCGCT TGGAAGCTAC AGGTGACGGA 
TTCCTGGAGC GGCCGTCTTC GCAGATGTCT TTCCTCGACC AGTTCCTCAA GGACACCTCG 
CGGCTCAATC ACAGCGAAAG CGGGGAGTCC GCCGGAGAAG CCGTTGCCAA CGACACGACA 
GAGTCTGAGA TGGAGAACCA GGTGCCGCCG CCGCCCATCG TCAGGTCCAG CACCTCCACT 
GAGGAACGGC GAGGAATCAA AATCTCCTCC CCGATTAGCG ACGCGCGTCC CACAATCACG 
GTTCTCACGC CCAAGCCCAA GGCGCACCAG TTCCTGGTGC GGACGTTTGT GGCGCCCCTC 
AAGTGCAACC ACTGCACCTC CCTCATGGTG GGCCTCATCC GGCAAGGGGC TGTGTGCGAG 
GTGTGTGGCT TTGCCTGCCA CGTCACGTGT CAAGACAAGG TGCCTGCCGT GTGTCCAGTG 
CCAATGGATC AGACAAAAAG GCCAGTTGGA ATCGACCCAA CAAGAGGCGT GGGAACAGCC 
TACGAAGGAT ATGTCAAGGT GCCCAAACTG GGCGGCGTGA AGAAAGGCTG GCAGAGGCAG 
TTTGTGGCTG TCTGCGACTT CAAGCTCTTC CTGTACGACC TGCTGCAGGA GAAGAACGTC 
CAGCCGGCGG TGTCTGCCTC CCAGGTGCTC GACATGAGGG ACGAGGAATT CTCGGTCACC 
TCCGTGCTCG AGTCGGACGT GATCCACGCC AACCGGAAGG ACATCCCGTG CATCTTTCGA 
GTGTCGACGT CCATGATGGA TCCCCCCGGG CTGCGCAGCC AGACGCTGAT GATGGTGGAC 
AAGGAGAGCG AGAAGGTCAA GTGGGTGGAC GCCCTCAATG AGCTGCACCG CATCCTCCGC 
CGGAACAAGC TGCCCCACAG GACGGTGTTC CAAGCCAAGG AGATTCTGGA CAGCACCCTC 
GTGATTATCA AGAATGCCTT ATCAGCCACT GTGATAGACC CGGACCGGAT AGCGCTGGGA 
ACCGAGGAGG GGCTGTACTG CGTCGACCTG GACCGAGAAG AGATTGCTCG GATCGGGGAC 
GGCAAGAGGA TCGCTCAGCT CGAGTACATT CCTGATGAGC AGTTGCTCAT TGCCATAGCA 
GGCAAGCAGC GACACTTGCG GCTGATTCCC GTGGGGGCAC TGGACGGCCA CGAGGTCGAC 
TGGATCAAGG TGGCGGACAC CAAGGGCTGC TCGAGCTTCT GCACCACCCG CATGGACCAT 
GGGGGCTCGT CTACTTACTA CTTCTGTGTG GCCGTCAAGA AGCAGGTGCT GGTACACCAA 
GTGAACCGCA CCAAGAGCCG GCACGGGGGC CGGCAGGTGA TCAGCGTGCC CGGGGTGGCC 
CAGTGCCTGG ACGTCTGGCA AGACCGCCTC TGCGTGGGGC TGCCCTCCCT CTTCTACCTT 
TACAGCCTAC AGGGCACCAC CGAGCCCATC TGCCTGGTGC AGCCGGACAA CAACCTGTCC 
TTCCTGTACC ACAACCCGAT GGATGCCTTC ATGGCTGTGG AGCTCCCCAA CAACGAGTAC 
CTACTCGTCT TCAGCCAGCT TGGAGTGTTT GTGAACAACC TTGGGAAGAA GACCCGGGAG 
AAAGAAGTTA TGTTCTCGGC CTTACCGCTG GCAGTCAGTT ACCACGACTC CTACCTGTGC 
GTCTACATGG AGAGCCACGT GGATGTGTAC GAGGTGACCT CTGGGGACTG GGTGCAGACC 
ATGAACCTCA AGAAGACGAA GCCCCTGTGC CGGCAGGGCC AGCTGTGCTT CTCGCTGACG 
ACGGAGGCGC CCCAGGTGCT GTACCTGCAC CCCGTGCACC GGAGGGAGCA CGCCGTGAGG 
GTGGAGCCCC ACCGGCCAGG GGCCCCCCAC GCCCTGGGGC CCCTCAGGGG TTCCTCGTCC 
TCCGTCGCTG CCTCCCTGGT CACCAGGGCC CGCAGGAGGT TCTCCGTGCG CGAGCAGGAG 
AAGCTGCCCA AGGGGGACCG CCGGTCGAGG ATGATCTCTG CACCCTCGAA CTTCAACCAC 
ATCCAACACA TGGGACCCAG CGAGCTCCAG AAACTCATCG ACCTGCCCAC GACGGCACAG 
GGGGTTGCAG CCATGGAGGA GAAGAGGGCT TGGGTGAAGT CTGTGAAACC TCCAGGTCTG 
TTTCAAGCGT CACACCTCGA AAGCAGAACC CAAGGGGTGC CCCTGCAGCC CAAGCGGCCC 
GCCCCCCTGG CTCCCGGCCA CAGAACTGCC AAGTCGTCGC TGACAGCCTC TCCCGACGGA 
TCGCTCTCTT CTCAGGAGCA CACCTCCGGT CCGCAGGCCT CCTCGGCTCA CGACGACTCT 
GGGCACCAGT CGAGTCCGCG GCACTCCATC GCCTCCAACA ACAGCTCCAA CTTGAGCAGC 
CCCCCAAGTC CGGTGGATCT CGGCCTTGGC TTGGAAGATG CGCGCGAGCA GTTCTCCTCG 
AGCTACGAGT CGCAGAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029822572.1
CDS	650..5890
Translation	MSKKGDCRCPEERIRELEQMFLGGPVLASGKAFTVEGLIDVLVVLYDECCNSSLRKEKTMTNFIEYVKPVVQRIKDLRLTKDDFDVLKVIGRGAFGEVAVVRERNTGQVYAMKILNKWEMLKRAETACFQEERDVLVFGDRRWITNLHYSFQDETNLYLVMDYYCGGDLLTLLSKFEDKLPEEMSRFYITEMILAVDSIHSLHYVHRDIKPDNVLLDANGHIRLADFGSCLRLCDDGTVQSNVAVGTPDYISPEILRAMEDGQGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKNCFDFPDDPGCSVSEDAKDLMRRLICSADCRLGQNGLEDFKRHPWFKGVDWDHVRQSQAPYIPEVSSPTDTSNFDVDEADLKQPESGPPSANAVFSGLHLPFVGFTYTLGSRLAQGEETAVDDSHVVCRRKIQALEREKQDMCQRMARDLKRGLPNSGGGGDVVLRNSSTTDGEVRKLQDEVNTFRKKNAELQLELEQHQPLSNSHGGHSVGVGDVKVKELEKALRLARAEKDDIHRDLTEAQEKLKQQTKELKDALAQRKLAMDEYTEVSDKLSDLRTQKQKLSRQVRDKEEELEKAMHKIDSLRQDLRKADKLRRELEARAEETQAEALKERRLRERSDEYVHHLEGEVEALRQCQLGRLSTQGTDVSQEAARLKSELENQEVKFRELEIQLQTRHATELNSVRDQLREADSARDTLFKEVMMLKEKNEKYRAESAMESQETLAEIKKAHEREKAMLSEEVRKLTREGEQLAETVRRLHEERRCFEEEMGQLREKKESVAQWEAQISEIIQWVSDEKDARGYLQALATKMTEELDYLRMSGLPTPTAEKNWRNRRSQKLEKMELLTLQSSLQSEIQAKQVVCEELSRVRAELVAQQKEHRKIQQEFEHFRKESTKKDSQIRDLQQRLEATGDGFLERPSSQMSFLDQFLKDTSRLNHSESGESAGEAVANDTTESEMENQVPPPPIVRSSTSTEERRGIKISSPISDARPTITVLTPKPKAHQFLVRTFVAPLKCNHCTSLMVGLIRQGAVCEVCGFACHVTCQDKVPAVCPVPMDQTKRPVGIDPTRGVGTAYEGYVKVPKLGGVKKGWQRQFVAVCDFKLFLYDLLQEKNVQPAVSASQVLDMRDEEFSVTSVLESDVIHANRKDIPCIFRVSTSMMDPPGLRSQTLMMVDKESEKVKWVDALNELHRILRRNKLPHRTVFQAKEILDSTLVIIKNALSATVIDPDRIALGTEEGLYCVDLDREEIARIGDGKRIAQLEYIPDEQLLIAIAGKQRHLRLIPVGALDGHEVDWIKVADTKGCSSFCTTRMDHGGSSTYYFCVAVKKQVLVHQVNRTKSRHGGRQVISVPGVAQCLDVWQDRLCVGLPSLFYLYSLQGTTEPICLVQPDNNLSFLYHNPMDAFMAVELPNNEYLLVFSQLGVFVNNLGKKTREKEVMFSALPLAVSYHDSYLCVYMESHVDVYEVTSGDWVQTMNLKKTKPLCRQGQLCFSLTTEAPQVLYLHPVHRREHAVRVEPHRPGAPHALGPLRGSSSSVAASLVTRARRRFSVREQEKLPKGDRRSRMISAPSNFNHIQHMGPSELQKLIDLPTTAQGVAAMEEKRAWVKSVKPPGLFQASHLESRTQGVPLQPKRPAPLAPGHRTAKSSLTASPDGSLSSQEHTSGPQASSAHDDSGHQSSPRHSIASNNSSNLSSPPSPVDLGLGLEDAREQFSSSYESQS

Target ORF information:

RefSeq Version	XM_029966712.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis serine/threonine-protein kinase MRCK alpha (LOC8026993), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029966712.1

ORF Insert Sequence:

ATGTCCAAGA AAGGGGACTG CCGCTGCCCC GAGGAGCGGA TACGCGAGCT CGAACAAATG 
TTCCTCGGAG GGCCCGTGCT GGCCAGCGGC AAGGCCTTCA CCGTCGAGGG CCTCATCGAC 
GTGCTGGTGG TGCTTTACGA CGAGTGCTGC AACTCGTCGC TACGCAAGGA GAAGACAATG 
ACCAACTTCA TCGAGTACGT CAAACCTGTG GTTCAGAGGA TCAAAGACCT GAGGCTGACC 
AAGGACGACT TCGACGTGCT CAAGGTGATA GGAAGGGGAG CGTTTGGAGA GGTGGCTGTG 
GTGCGAGAGC GGAACACGGG CCAAGTGTAC GCCATGAAGA TCCTCAACAA GTGGGAAATG 
CTCAAGAGGG CGGAGACGGC CTGTTTCCAA GAAGAGCGTG ACGTCCTCGT TTTCGGAGAC 
AGGCGGTGGA TCACGAATCT GCACTACTCC TTTCAAGATG AGACCAACTT GTACCTGGTA 
ATGGACTACT ACTGTGGCGG CGACCTGCTC ACGCTGCTGA GCAAGTTCGA GGACAAGCTG 
CCCGAGGAGA TGTCCCGCTT CTACATCACG GAGATGATCC TGGCCGTGGA CTCCATCCAC 
TCGCTCCACT ACGTCCACCG GGACATCAAG CCAGACAACG TGCTGCTGGA CGCCAACGGA 
CACATTCGCC TGGCCGACTT TGGCTCCTGC CTCAGGCTGT GCGACGACGG AACGGTTCAG 
TCCAACGTTG CGGTCGGCAC CCCTGACTAC ATATCTCCCG AAATCCTAAG GGCGATGGAA 
GATGGGCAGG GCAGGTACGG CCCGGAGTGC GACTGGTGGT CCCTGGGCGT CTGCATGTAC 
GAGATGCTGT ACGGGGAGAC GCCTTTCTAC GCCGAGTCCC TCGTGGAGAC CTACGGCAAG 
ATCATGAACC ACAAGAACTG CTTCGACTTC CCGGACGATC CGGGCTGCTC GGTGTCGGAG 
GATGCCAAGG ACCTGATGCG GCGGCTCATC TGCAGCGCGG ACTGCCGGCT GGGTCAGAAC 
GGCCTGGAGG ACTTCAAGCG CCACCCCTGG TTCAAAGGGG TCGACTGGGA CCACGTACGC 
CAGAGCCAGG CGCCCTACAT TCCGGAGGTG AGCAGTCCGA CGGACACCTC CAACTTCGAC 
GTGGACGAGG CCGACCTCAA GCAGCCCGAG TCCGGCCCCC CGAGCGCCAA TGCGGTGTTC 
TCTGGGCTGC ACCTGCCCTT TGTGGGCTTC ACGTACACCC TGGGCAGCCG GCTGGCCCAG 
GGGGAGGAGA CGGCCGTGGA CGACAGCCAC GTCGTCTGCC GCAGGAAGAT CCAGGCCCTG 
GAGCGGGAGA AGCAGGACAT GTGCCAGAGG ATGGCCAGGG ACCTCAAGAG GGGGCTCCCC 
AACTCTGGCG GCGGAGGTGA CGTGGTGCTG CGCAACAGCT CCACCACGGA CGGGGAGGTG 
CGCAAGCTGC AGGACGAGGT GAACACCTTC CGGAAGAAGA ATGCTGAACT GCAGCTAGAG 
CTGGAGCAGC ACCAGCCCCT GTCCAACTCG CACGGCGGCC ACTCCGTCGG GGTGGGCGAC 
GTCAAGGTCA AGGAGCTGGA GAAGGCGCTG CGTCTCGCAC GGGCGGAGAA GGATGACATC 
CACCGCGACC TGACAGAAGC CCAAGAGAAG CTGAAGCAGC AGACAAAGGA ACTGAAGGAT 
GCCCTAGCCC AGAGGAAACT AGCCATGGAT GAATACACCG AAGTCAGCGA CAAGCTGTCC 
GACCTGCGCA CCCAGAAGCA GAAGCTGTCG CGGCAGGTGC GGGACAAGGA GGAGGAACTG 
GAGAAGGCCA TGCACAAGAT CGACAGCCTC CGGCAAGACC TCAGGAAGGC CGACAAGCTG 
CGCAGAGAAC TGGAGGCCCG GGCGGAGGAG ACCCAGGCGG AAGCCCTGAA GGAGCGGCGC 
TTGAGAGAGC GCAGCGACGA GTACGTGCAC CACCTGGAGG GGGAAGTGGA GGCCCTGCGC 
CAATGCCAGC TGGGACGCCT CTCCACCCAG GGCACGGACG TCTCCCAGGA GGCCGCCAGG 
TTGAAGAGTG AGCTGGAGAA CCAAGAGGTG AAGTTCCGGG AGCTGGAGAT TCAGCTGCAG 
ACGAGGCACG CGACCGAGCT GAACTCGGTG CGTGACCAGC TCCGCGAAGC CGACAGCGCC 
CGGGACACCC TCTTCAAGGA GGTGATGATG CTCAAGGAGA AGAATGAAAA GTACCGGGCT 
GAAAGCGCCA TGGAGAGCCA GGAGACCCTG GCGGAGATCA AGAAGGCCCA CGAGCGGGAG 
AAGGCCATGC TCTCCGAAGA GGTCCGCAAG CTCACCCGGG AAGGGGAGCA GCTGGCGGAG 
ACTGTGAGGA GACTGCACGA GGAGCGGAGG TGCTTCGAAG AGGAGATGGG CCAGCTGCGG 
GAGAAGAAGG AGTCCGTCGC TCAGTGGGAG GCACAGATCA GCGAGATCAT CCAGTGGGTG 
AGCGACGAAA AGGATGCCCG TGGCTACCTG CAAGCCCTGG CCACTAAGAT GACGGAGGAG 
CTGGACTACC TGAGGATGAG CGGACTGCCC ACGCCCACTG CGGAGAAGAA CTGGCGGAAC 
CGGCGGTCCC AGAAGCTGGA AAAGATGGAG CTGCTCACCC TGCAGTCGAG CCTCCAGAGC 
GAGATCCAGG CCAAGCAGGT GGTCTGCGAG GAGCTGAGCC GGGTGCGGGC CGAGCTGGTC 
GCCCAGCAGA AGGAGCACCG CAAGATTCAG CAGGAGTTTG AGCACTTCCG GAAGGAGTCT 
ACCAAGAAGG ACTCCCAGAT CCGAGACCTG CAGCAGCGCT TGGAAGCTAC AGGTGACGGA 
TTCCTGGAGC GGCCGTCTTC GCAGATGTCT TTCCTCGACC AGTTCCTCAA GGACACCTCG 
CGGCTCAATC ACAGCGAAAG CGGGGAGTCC GCCGGAGAAG CCGTTGCCAA CGACACGACA 
GAGTCTGAGA TGGAGAACCA GGTGCCGCCG CCGCCCATCG TCAGGTCCAG CACCTCCACT 
GAGGAACGGC GAGGAATCAA AATCTCCTCC CCGATTAGCG ACGCGCGTCC CACAATCACG 
GTTCTCACGC CCAAGCCCAA GGCGCACCAG TTCCTGGTGC GGACGTTTGT GGCGCCCCTC 
AAGTGCAACC ACTGCACCTC CCTCATGGTG GGCCTCATCC GGCAAGGGGC TGTGTGCGAG 
GTGTGTGGCT TTGCCTGCCA CGTCACGTGT CAAGACAAGG TGCCTGCCGT GTGTCCAGTG 
CCAATGGATC AGACAAAAAG GCCAGTTGGA ATCGACCCAA CAAGAGGCGT GGGAACAGCC 
TACGAAGGAT ATGTCAAGGT GCCCAAACTG GGCGGCGTGA AGAAAGGCTG GCAGAGGCAG 
TTTGTGGCTG TCTGCGACTT CAAGCTCTTC CTGTACGACC TGCTGCAGGA GAAGAACGTC 
CAGCCGGCGG TGTCTGCCTC CCAGGTGCTC GACATGAGGG ACGAGGAATT CTCGGTCACC 
TCCGTGCTCG AGTCGGACGT GATCCACGCC AACCGGAAGG ACATCCCGTG CATCTTTCGA 
GTGTCGACGT CCATGATGGA TCCCCCCGGG CTGCGCAGCC AGACGCTGAT GATGGTGGAC 
AAGGAGAGCG AGAAGGTCAA GTGGGTGGAC GCCCTCAATG AGCTGCACCG CATCCTCCGC 
CGGAACAAGC TGCCCCACAG GACGGTGTTC CAAGCCAAGG AGATTCTGGA CAGCACCCTC 
GTGATTATCA AGAATGCCTT ATCAGCCACT GTGATAGACC CGGACCGGAT AGCGCTGGGA 
ACCGAGGAGG GGCTGTACTG CGTCGACCTG GACCGAGAAG AGATTGCTCG GATCGGGGAC 
GGCAAGAGGA TCGCTCAGCT CGAGTACATT CCTGATGAGC AGTTGCTCAT TGCCATAGCA 
GGCAAGCAGC GACACTTGCG GCTGATTCCC GTGGGGGCAC TGGACGGCCA CGAGGTCGAC 
TGGATCAAGG TGGCGGACAC CAAGGGCTGC TCGAGCTTCT GCACCACCCG CATGGACCAT 
GGGGGCTCGT CTACTTACTA CTTCTGTGTG GCCGTCAAGA AGCAGGTGCT GGTACACCAA 
GTGAACCGCA CCAAGAGCCG GCACGGGGGC CGGCAGGTGA TCAGCGTGCC CGGGGTGGCC 
CAGTGCCTGG ACGTCTGGCA AGACCGCCTC TGCGTGGGGC TGCCCTCCCT CTTCTACCTT 
TACAGCCTAC AGGGCACCAC CGAGCCCATC TGCCTGGTGC AGCCGGACAA CAACCTGTCC 
TTCCTGTACC ACAACCCGAT GGATGCCTTC ATGGCTGTGG AGCTCCCCAA CAACGAGTAC 
CTACTCGTCT TCAGCCAGCT TGGAGTGTTT GTGAACAACC TTGGGAAGAA GACCCGGGAG 
AAAGAAGTTA TGTTCTCGGC CTTACCGCTG GCAGTCAGTT ACCACGACTC CTACCTGTGC 
GTCTACATGG AGAGCCACGT GGATGTGTAC GAGGTGACCT CTGGGGACTG GGTGCAGACC 
ATGAACCTCA AGAAGACGAA GCCCCTGTGC CGGCAGGGCC AGCTGTGCTT CTCGCTGACG 
ACGGAGGCGC CCCAGGTGCT GTACCTGCAC CCCGTGCACC GGAGGGAGCA CGCCGTGAGG 
GTGGAGCCCC ACCGGCCAGG GGCCCCCCAC GCCCTGGGGC CCCTCAGGGG TTCCTCGTCC 
TCCGTCGCTG CCTCCCTGGT CACCAGGGCC CGCAGGAGGT TCTCCGTGCG CGAGCAGGAG 
AAGCTGCCCA AGGGGGACCG CCGGTCGAGG ATGATCTCTG CACCCTCGAA CTTCAACCAC 
ATCCAACACA TGGGACCCAG CGAGCTCCAG AAACTCATCG ACCTGCCCAC GACGGCACAG 
GGGGTTGCAG CCATGGAGGA GAAGAGGGCT TGGGTGAAGT CTGTGAAACC TCCAGGTCTG 
TTTCAAGCGT CACACCTCGA AAGCAGAACC CAAGGGGTGC CCCTGCAGCC CAAGCGGCCC 
GCCCCCCTGG CTCCCGGCCA CAGAACTGCC AAGTCGTCGC TGACAGCCTC TCCCGACGGA 
TCGCTCTCTT CTCAGGAGCA CACCTCCGGT CCGCAGGCCT CCTCGGCTCA CGACGACTCT 
GGGCACCAGT CGAGTCCGCG GCACTCCATC GCCTCCAACA ACAGCTCCAA CTTGAGCAGC 
CCCCCAAGTC CGGTGGATCT CGGCCTTGGC TTGGAAGATG CGCGCGAGCA GTTCTCCTCG 
AGCTACGAGT CGCAGAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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1-732-210-0262

LOC8026993 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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