LOC8029650 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC8029650
Entrez Gene ID	8029650
Full Name	cytoskeletal protein Sojo, putative
Gene Type	protein-coding
Organism	Ixodes scapularis(black-legged tick)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029995331.1	XP_029851191.1	rootletin isoform X1
XM_029995334.1	XP_029851194.1	rootletin isoform X4
XM_029995333.1	XP_029851193.1	rootletin isoform X3
XM_002406583.1	XP_002406627.1	cytoskeletal protein Sojo, putative
XM_029995332.1	XP_029851192.1	rootletin isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC8029650 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC8029650 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OIa03670	XM_002406583.1 Latest version!	Ixodes scapularis cytoskeletal protein Sojo, putative, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OIa36134	XM_029995331.1 Latest version!	Ixodes scapularis rootletin (LOC8029650), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIa36135	XM_029995332.1 Latest version!	Ixodes scapularis rootletin (LOC8029650), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIa36136	XM_029995333.1 Latest version!	Ixodes scapularis rootletin (LOC8029650), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OIa36137	XM_029995334.1 Latest version!	Ixodes scapularis rootletin (LOC8029650), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC8029650 ( XM_002406583.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_002406627.1 Ixodes scapularis cytoskeletal protein Sojo, putative, mRNA.

Price & Availability↑

CloneID	OIa03670
Clone ID Related Accession (Same CDS sequence)	XM_002406583.1
Accession Version	XM_002406583.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2009-06-22
Organism	Ixodes scapularis(black-legged tick)
Product	cytoskeletal protein Sojo, putative
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002615885). COMPLETENESS: incomplete on both ends.

ATGTCTGGTC GGCTCGTGCT CCGCAGATGT GCGGAGCTGA AGCAGCTGAA CGTGGTCCTC 
CGCGAGCACC TGGAGATAAC GACGGAGACG AATGCGACCC TCACCATGCA CATGGAGAGG 
ATGACGGAGG ACTGGCAGAA CGCGACCCGG GCTGACGTGG CCAATCGCGG CAGCAGCAGC 
AGCGCCACCC ACGACGAGCT GTCCTCTCAG AAGGACGCGC TCTCGCAAGA AGCGTCCAGC 
CTTCGGCAGA GTGTCGCGAG CCAGCGGGAG ACAAACGAGC AGCTCAGCGC ACAGAAGGAA 
GAGCTGGCCA CGGCCAAGTC TCAGCTGGAA ATGGAGCTAC GGCACTGCCG GGCCGAGGGG 
GACAAGCTCC AGAGCGACCT GACTGGCTGC AAGGAAGCCC GGGAAGCGAC AGAAGTGGAC 
CTGCGGAACG CCAGGAATTC CCTCGCAAAG ATCCAGCGGG AGAAAGAGAC GACGGACGCC 
AAGCTTCGGG ATTCAGCGGC AGAGCTGGAG AAATTGCACG GCTGGCCAAT TGCGGCAACT 
CGAGGAAAGC CACGTAGCAG AACTGAAGCG CCAGGAAGAG CTGCTGAGCG AGACAAAGAC 
CAAGTGGCAC AGCGAGGTGG CCCTGCACAG CAGAAGCTGG AAGACGTGAG GGTGGAGCTC 
CAGGAAAAGC TAGCTTCTTC CGAGCAGCTC AGAGCCGAGA TGCAGTCTAT GGCGGAGGAC 
GAGCGCAGGG AGCTGCTGAA CTCTCTCGGC CGCTGCAAGG AGGAAGTGTC CGTGCTCCGG 
CGAAACCTGG TGGCATTCAG GGATGAAGCA GGCTCCAAGA TGGCCGCCGA GCAGGCCAAG 
GTGGCGTCTG CTCTGAAGGA CGTCGACACA CTTCGCGTGG AACTGGAGCA GACTAGGACG 
GCGTTGCGTT TGAAAGAGGC ACAGGAAAAG TCACGTGAGT CGCAGTTTCA CAAGGAACTC 
AGCCGGGTGA CCGAAGAAAG GGAGCGTGCC ATTCAGCGGC TGAACGAGGC CGATGCCAGT 
CACGCGGCTC TTCAGCGCCA GCTGTCTGAA GCTCTTGGCA AGAAATCCGA AGGGGAGCGT 
CGAGTTGAAG TTCTGACTCA CCAGTTATCC GAGGCGCGTC AGAGTTACAC GTTGGTCACG 
GCAGAGACCG AACAGCTTCG CCGAGTCATT GGGGATTTGC AGCAGGAGTT GCGCAGCACC 
ACTCAGGAGA GGGACGTCTC GCTCCAGGAG GCTTCCGAAA AATTCCGTGG CGCGGAGAAA 
GCGCGGGCGA CGTTGGCGGA GGAGCTCCGC GAGCTCCGGA ACACGCACAG CGGCGTGGAG 
CGGGCTCGGC AGGTCTTGGA GCGCAAGGTG CGAGCCCTGG AGTCGGCTTT GCTGGAGCAC 
GGCTCGGAGC TGGACTTGCT CCGGAAGCTG TCCGAGCAGA GGGGACATCA AGTTGCCGTG 
CTCGAGGATG AGCGGGACGC GTGGCAGCGC GAGCGTGCCT CTTTGGGGCG TCGCCTGGAC 
GAGAGCGAGC GCCGTGCACA GGCGGAGCGC AACGCTCGCG AAGGGGCGGT GCGCGAGGCC 
GGCCGGGTTG CCGAGAGGCT GGCCCAGGAC CGGGCCGACC TGGAGAAGCA GCTGCTGGCA 
TCTGGGCAGG CAACGCACGA GGCACGCCTG GCACTGTGTG CCGCGCAGGG CTCCACGGCT 
GCCCTCGAGA GCGAGCTCAA GATCGTCTCC GAGTCTCGGA AGTCTATCGA GGGCACTCTG 
GACTCGCTGC TGAGCGTGCT GCGCTGCTCC CTGGGGATCC AGACCAAGTA CGAGCTGCGG 
TCCCCCTCCC CCCAGCGACC GGCGGCAAGC CGGCCCCACG GCGGCGACCT GTCCAACGAG 
AGCTTTCGGA GGGACAGCTC GCCCTTTCGG TCAGAGGACT CCCTCGGCCG CTCCCTGCTC 
CGGCGGCCCA CCCCCGACGT CGAGGCCGAG CTCGTGCTAC AAGCCGTGGA CGCCCTGTCG 
CACCGCATCG CCGCGCTTGA AGCCGACAAG GAGAAGGTGG AGGCCGAGTG CGAGCGTTCC 
CAGCGGGTGG TTGCCGGACT GGAGGACGAG CTCGCGGCCA CAAAGTCCAC GCTTGTGCGA 
CTGACCAAGG CAAACGATGC TCTCGTTGCA GACAATGCGG AGCTGCGCTC GCAAGTGTCC 
TCTCAGCTGG AGGCGGTCTC GGAACTCAAA CACGCGTCCC AGACCCTGGA GTGGGAGAAA 
GCATCTCTCG CGGACCAGCT GGCAGACGCG GCGCGCCGCT ACCAGCTCAA GACGGACCAG 
TGCGACAAGC TGCAGGCCGC CCTGAGTCGC AGCCGCGACC ACCCACCATC CCCGATCCGG 
CCCTCCGAGC CCCTCGAGTC TCGGCTGTCG AAGCTCGAGC TCGAGCGACA ACTCTCGCAA 
GCCAAGGTGG AGAGGCTCAA GCTCAGGGTG ACCGAGAGCC AGGCCGAAGT GAGGAGGCTC 
CAGGAAGCCA AGGAGGAAGC CACTTCCCAG CTGACTCGGC TCGAGAGGAG GGCGGAGAGC 
CTCGACCACG ACAACAGCCG CCTGAGGGCA GAGATTGCGG AGCATGCGTC CAAGGTGAAG 
GTCCTGAATG CTCACCTGGT GGAAGAGATC GCCAAGGGAC GCGGCCTGGA GGAGGAGAAG 
CGATCTCTGG AAAGCCAGCT CAGGGTGGCC AGGGTGCAGC TCGAGTCTGC CAGGTTTGAC 
CGGAAAAGAG ATGGGTCGGT GATTGACGAG CTGAAGGGCC AGAAAGGCAC CCTGGAGACC 
AGGCTGTCTG CGCTCGCCGT ACAGCTGGCA GAATGCGAGT CGGCCAAGCA GCGCCTCGAC 
AAGACGCCCA TCAGGACTCC CCACAGGGAA GAGACCAGGA CTTGGCAGCA GATGGTGGAG 
ACGCTGCGCG AGGAGAAGCG GCAGCTCCAG GGGGAGGTGA CGCGCCTCCG GCGGCAGCTC 
ACGGGCACCA TTGACAGTAT CCTGCCGCCG GCGACTCCCG ACGGGAGCCG AGCTTCGAGG 
CGGGCACGCC GCAGTCTGGC GGAGCGTGAC GTGCCAGACG GCGCAAGCCT CCATTCTCCC 
GAGGTGTCTC GCCTGAAGAC GCAGAAGAGC CTCCTGGAGG CCAAAGTCCG CACTTTGGAG 
GAGCGCCTCT CTCAGCAAGA CCAGGTGCAC TCGGAGGAGC TGACGCGAGA ACATCGGGAG 
CTGATCGCCG AGATCGCCAG ACTGCGGGAG GAGCTGGCAA CGCACGAGCG GCGTCGGACG 
TCGACCCCCG TGCACAACGA GGTAGGCGGC GCCACCTGGA AGCACTCCCT GTTGAACCCC 
CTGCTCAAAC TCTGCCTGCT CGAGACCTCT GCCTTGCTCC TGGGGCTGCT GCTCTGCCTC 
CTGCACATCC TGCTTGCCCC CGAGGCGCCC GCGTCCGACC GGGAAGCCTA CCTGCTCGAG 
TCTGTGGCGT CGGGCGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002406627.1
CDS	1..3441
Translation	MSGRLVLRRCAELKQLNVVLREHLEITTETNATLTMHMERMTEDWQNATRADVANRGSSSSATHDELSSQKDALSQEASSLRQSVASQRETNEQLSAQKEELATAKSQLEMELRHCRAEGDKLQSDLTGCKEAREATEVDLRNARNSLAKIQREKETTDAKLRDSAAELEKLHGWPIAATRGKPRSRTEAPGRAAERDKDQVAQRGGPAQQKLEDVRVELQEKLASSEQLRAEMQSMAEDERRELLNSLGRCKEEVSVLRRNLVAFRDEAGSKMAAEQAKVASALKDVDTLRVELEQTRTALRLKEAQEKSRESQFHKELSRVTEERERAIQRLNEADASHAALQRQLSEALGKKSEGERRVEVLTHQLSEARQSYTLVTAETEQLRRVIGDLQQELRSTTQERDVSLQEASEKFRGAEKARATLAEELRELRNTHSGVERARQVLERKVRALESALLEHGSELDLLRKLSEQRGHQVAVLEDERDAWQRERASLGRRLDESERRAQAERNAREGAVREAGRVAERLAQDRADLEKQLLASGQATHEARLALCAAQGSTAALESELKIVSESRKSIEGTLDSLLSVLRCSLGIQTKYELRSPSPQRPAASRPHGGDLSNESFRRDSSPFRSEDSLGRSLLRRPTPDVEAELVLQAVDALSHRIAALEADKEKVEAECERSQRVVAGLEDELAATKSTLVRLTKANDALVADNAELRSQVSSQLEAVSELKHASQTLEWEKASLADQLADAARRYQLKTDQCDKLQAALSRSRDHPPSPIRPSEPLESRLSKLELERQLSQAKVERLKLRVTESQAEVRRLQEAKEEATSQLTRLERRAESLDHDNSRLRAEIAEHASKVKVLNAHLVEEIAKGRGLEEEKRSLESQLRVARVQLESARFDRKRDGSVIDELKGQKGTLETRLSALAVQLAECESAKQRLDKTPIRTPHREETRTWQQMVETLREEKRQLQGEVTRLRRQLTGTIDSILPPATPDGSRASRRARRSLAERDVPDGASLHSPEVSRLKTQKSLLEAKVRTLEERLSQQDQVHSEELTREHRELIAEIARLREELATHERRRTSTPVHNEVGGATWKHSLLNPLLKLCLLETSALLLGLLLCLLHILLAPEAPASDREAYLLESVASGA

Target ORF information:

RefSeq Version	XM_002406583.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis cytoskeletal protein Sojo, putative, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002406583.1

ORF Insert Sequence:

ATGTCTGGTC GGCTCGTGCT CCGCAGATGT GCGGAGCTGA AGCAGCTGAA CGTGGTCCTC 
CGCGAGCACC TGGAGATAAC GACGGAGACG AATGCGACCC TCACCATGCA CATGGAGAGG 
ATGACGGAGG ACTGGCAGAA CGCGACCCGG GCTGACGTGG CCAATCGCGG CAGCAGCAGC 
AGCGCCACCC ACGACGAGCT GTCCTCTCAG AAGGACGCGC TCTCGCAAGA AGCGTCCAGC 
CTTCGGCAGA GTGTCGCGAG CCAGCGGGAG ACAAACGAGC AGCTCAGCGC ACAGAAGGAA 
GAGCTGGCCA CGGCCAAGTC TCAGCTGGAA ATGGAGCTAC GGCACTGCCG GGCCGAGGGG 
GACAAGCTCC AGAGCGACCT GACTGGCTGC AAGGAAGCCC GGGAAGCGAC AGAAGTGGAC 
CTGCGGAACG CCAGGAATTC CCTCGCAAAG ATCCAGCGGG AGAAAGAGAC GACGGACGCC 
AAGCTTCGGG ATTCAGCGGC AGAGCTGGAG AAATTGCACG GCTGGCCAAT TGCGGCAACT 
CGAGGAAAGC CACGTAGCAG AACTGAAGCG CCAGGAAGAG CTGCTGAGCG AGACAAAGAC 
CAAGTGGCAC AGCGAGGTGG CCCTGCACAG CAGAAGCTGG AAGACGTGAG GGTGGAGCTC 
CAGGAAAAGC TAGCTTCTTC CGAGCAGCTC AGAGCCGAGA TGCAGTCTAT GGCGGAGGAC 
GAGCGCAGGG AGCTGCTGAA CTCTCTCGGC CGCTGCAAGG AGGAAGTGTC CGTGCTCCGG 
CGAAACCTGG TGGCATTCAG GGATGAAGCA GGCTCCAAGA TGGCCGCCGA GCAGGCCAAG 
GTGGCGTCTG CTCTGAAGGA CGTCGACACA CTTCGCGTGG AACTGGAGCA GACTAGGACG 
GCGTTGCGTT TGAAAGAGGC ACAGGAAAAG TCACGTGAGT CGCAGTTTCA CAAGGAACTC 
AGCCGGGTGA CCGAAGAAAG GGAGCGTGCC ATTCAGCGGC TGAACGAGGC CGATGCCAGT 
CACGCGGCTC TTCAGCGCCA GCTGTCTGAA GCTCTTGGCA AGAAATCCGA AGGGGAGCGT 
CGAGTTGAAG TTCTGACTCA CCAGTTATCC GAGGCGCGTC AGAGTTACAC GTTGGTCACG 
GCAGAGACCG AACAGCTTCG CCGAGTCATT GGGGATTTGC AGCAGGAGTT GCGCAGCACC 
ACTCAGGAGA GGGACGTCTC GCTCCAGGAG GCTTCCGAAA AATTCCGTGG CGCGGAGAAA 
GCGCGGGCGA CGTTGGCGGA GGAGCTCCGC GAGCTCCGGA ACACGCACAG CGGCGTGGAG 
CGGGCTCGGC AGGTCTTGGA GCGCAAGGTG CGAGCCCTGG AGTCGGCTTT GCTGGAGCAC 
GGCTCGGAGC TGGACTTGCT CCGGAAGCTG TCCGAGCAGA GGGGACATCA AGTTGCCGTG 
CTCGAGGATG AGCGGGACGC GTGGCAGCGC GAGCGTGCCT CTTTGGGGCG TCGCCTGGAC 
GAGAGCGAGC GCCGTGCACA GGCGGAGCGC AACGCTCGCG AAGGGGCGGT GCGCGAGGCC 
GGCCGGGTTG CCGAGAGGCT GGCCCAGGAC CGGGCCGACC TGGAGAAGCA GCTGCTGGCA 
TCTGGGCAGG CAACGCACGA GGCACGCCTG GCACTGTGTG CCGCGCAGGG CTCCACGGCT 
GCCCTCGAGA GCGAGCTCAA GATCGTCTCC GAGTCTCGGA AGTCTATCGA GGGCACTCTG 
GACTCGCTGC TGAGCGTGCT GCGCTGCTCC CTGGGGATCC AGACCAAGTA CGAGCTGCGG 
TCCCCCTCCC CCCAGCGACC GGCGGCAAGC CGGCCCCACG GCGGCGACCT GTCCAACGAG 
AGCTTTCGGA GGGACAGCTC GCCCTTTCGG TCAGAGGACT CCCTCGGCCG CTCCCTGCTC 
CGGCGGCCCA CCCCCGACGT CGAGGCCGAG CTCGTGCTAC AAGCCGTGGA CGCCCTGTCG 
CACCGCATCG CCGCGCTTGA AGCCGACAAG GAGAAGGTGG AGGCCGAGTG CGAGCGTTCC 
CAGCGGGTGG TTGCCGGACT GGAGGACGAG CTCGCGGCCA CAAAGTCCAC GCTTGTGCGA 
CTGACCAAGG CAAACGATGC TCTCGTTGCA GACAATGCGG AGCTGCGCTC GCAAGTGTCC 
TCTCAGCTGG AGGCGGTCTC GGAACTCAAA CACGCGTCCC AGACCCTGGA GTGGGAGAAA 
GCATCTCTCG CGGACCAGCT GGCAGACGCG GCGCGCCGCT ACCAGCTCAA GACGGACCAG 
TGCGACAAGC TGCAGGCCGC CCTGAGTCGC AGCCGCGACC ACCCACCATC CCCGATCCGG 
CCCTCCGAGC CCCTCGAGTC TCGGCTGTCG AAGCTCGAGC TCGAGCGACA ACTCTCGCAA 
GCCAAGGTGG AGAGGCTCAA GCTCAGGGTG ACCGAGAGCC AGGCCGAAGT GAGGAGGCTC 
CAGGAAGCCA AGGAGGAAGC CACTTCCCAG CTGACTCGGC TCGAGAGGAG GGCGGAGAGC 
CTCGACCACG ACAACAGCCG CCTGAGGGCA GAGATTGCGG AGCATGCGTC CAAGGTGAAG 
GTCCTGAATG CTCACCTGGT GGAAGAGATC GCCAAGGGAC GCGGCCTGGA GGAGGAGAAG 
CGATCTCTGG AAAGCCAGCT CAGGGTGGCC AGGGTGCAGC TCGAGTCTGC CAGGTTTGAC 
CGGAAAAGAG ATGGGTCGGT GATTGACGAG CTGAAGGGCC AGAAAGGCAC CCTGGAGACC 
AGGCTGTCTG CGCTCGCCGT ACAGCTGGCA GAATGCGAGT CGGCCAAGCA GCGCCTCGAC 
AAGACGCCCA TCAGGACTCC CCACAGGGAA GAGACCAGGA CTTGGCAGCA GATGGTGGAG 
ACGCTGCGCG AGGAGAAGCG GCAGCTCCAG GGGGAGGTGA CGCGCCTCCG GCGGCAGCTC 
ACGGGCACCA TTGACAGTAT CCTGCCGCCG GCGACTCCCG ACGGGAGCCG AGCTTCGAGG 
CGGGCACGCC GCAGTCTGGC GGAGCGTGAC GTGCCAGACG GCGCAAGCCT CCATTCTCCC 
GAGGTGTCTC GCCTGAAGAC GCAGAAGAGC CTCCTGGAGG CCAAAGTCCG CACTTTGGAG 
GAGCGCCTCT CTCAGCAAGA CCAGGTGCAC TCGGAGGAGC TGACGCGAGA ACATCGGGAG 
CTGATCGCCG AGATCGCCAG ACTGCGGGAG GAGCTGGCAA CGCACGAGCG GCGTCGGACG 
TCGACCCCCG TGCACAACGA GGTAGGCGGC GCCACCTGGA AGCACTCCCT GTTGAACCCC 
CTGCTCAAAC TCTGCCTGCT CGAGACCTCT GCCTTGCTCC TGGGGCTGCT GCTCTGCCTC 
CTGCACATCC TGCTTGCCCC CGAGGCGCCC GCGTCCGACC GGGAAGCCTA CCTGCTCGAG 
TCTGTGGCGT CGGGCGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8029650 ( XM_029995331.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029851191.1 Ixodes scapularis rootletin (LOC8029650), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OIa36134
Clone ID Related Accession (Same CDS sequence)	XM_029995331.1
Accession Version	XM_029995331.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	rootletin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021922528.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATATC TTGGCGACGG GCAAGTTCAG GCGGTTCAGG CACTCGGTGG TTCATTCCAA 
CATTCTGGTG GAGCGGTATT TGAAAGCCCG CTTCGCATTG CAGTGATGGA GCAGCCACCG 
GAGAACGTAA TTGCGAGGCT ACCGTCTTCG TCGACCATCA ATGACGAGGA CTATGCAACG 
GCAGAGGAGG AACCACCAGG CAGAACCTCG AGGCTCGAGG ACGACGACCA GCAGGGCGTG 
CTCTCGGACG CCTGCTCGAC CGACAGCGAC CACGAGCTCG AGTTGGTGGG TCTGGATCGC 
GAACACGAGC TCAAGTCGAA GCTGGAGAAG TCGAGGACCA TGTTGCGGGC CTACGTGCAG 
CACACGACCA ATCAGGACAG GCTGCTTGGG CACCTGGGAG CACAGGTGGA GGAGTACCGG 
AACCGCTGCT CGAGATTGGA AGAGGTCCTC CGCCAGTGCC CGTGTGCCGA TCGAACTGAG 
AACATTCAAG ACGATCGCTC GTACGAAGAA GATCCTCCTG ACCTTCCGTC GGCCTTATCA 
GCGCTGGAAC TAGAGAGGGC AAGATGTGCG GAGCTGAAGC AGCTGAACGT GGTCCTCCGC 
GAGCACCTGG AGATAACGAC GGAGACGAAT GCGACCCTCA CCATGCACAT GGAGAGGATG 
ACGGAGGACT GGCAGAACGC GACCCGGGCT GACGCGGTCA ATCGCGGCAG CAGCAGCAGC 
GCCACCCACG ACGAGTTTCG CGCAGACTCC GAGCAGGGCA GCGTTCCCAG GCTGCGTCGA 
GACTTGCAGG AGCTGAAGGG GGACCTGGGA GACATGAGGC AGGCCTTTGC CACCAGCCTG 
GCCTCCATCC AGACGATGCT GCACGACGCC GCCGAGGCGG CCGGGCGTGT CCAACTCTCG 
CTTGTGGACG ACGCGGCGGA CAGCGACCAG CCAGACCTCG GGCTCACCAG GTCTCGGGAC 
GAGGGCGTGG CGGGAGTCTC AGCGGCGCCG CTGGACTCTG TCGCCAAAAT GGTGACAGAC 
GACCTCAACC ACCAAGTGGA GGCGTTGCAT CGTGAAAACG AGGGCCTGCA CACGGTCCTG 
GCGAGCAGGC TGGAGACCAT CGAGCAGATG ACAAACGTCA TCGATGCGCT TAAGGCTGCG 
CAGTGTAACC TCGGAGCCAT ATCTGAGAAG AGCAGCCAGG AAAACGTGGA CCTGCGCGTG 
CGGGAAGCCA TGCTACAGTC GGAGGTGCGC AGCCACGAGG CCACGTCGAA GAATCTCGCC 
TTGGAGCTGG AGTCCGTCCA AAAGGAAAAG AAAGAGTGCA TGGGTGTAAT TGCGTCGCTG 
CGCAAGCATG TCGCCAACCT CGAGGACCAG CAACAGGTGT CCGAACGCGA GCTCAGCGTT 
CTGAGGAAAG CGGTGCGTGA CATCGAAGAA CTCAACGACT CCCTGGAACG GGAGATGCAA 
GAGATGTGCA ACGCCAGCAT GCAGCTTAAA CAGTCCAACT CGGAGCTGGC AAAGGCAAAG 
AGTGACATGG AACTTGCGCA CGAAGAGTTG GCCGCGGAAC GGGACTCCCT GGCACGACGG 
GAAGCGGATG TAACGCGAGC GATGGCCCGT CTGGAGTCGC TCTTGGAAGA GAGCGCGTCT 
CGGCAGACGG ATCTGGAGGC CACGCTCTCT GCCAGGGACA AGGAGATCGC CTACAGGAAG 
GCGGCGTTTC TTCAGCTGTC CTCTCAGAAG GACGCGCTCT CGCAAGAAGC GTCCAGCCTT 
CGGCAGAGTG TCGCGAGCCA GCGGGAGACA AACGAGCAGC TCAGCGCACA GAAGGAAGAG 
CTGGCCACGG CCAAGTCTCA GCTGGAAATG GAGCTACGGC ACTGCCGGGC CGAGGGGGAC 
AAGCTCCAGA GCGACCTGAC TGGCTGCAAG GAAGCCCGGG AAGCGACAGA AGTGGACCTG 
CGGAACGCCA GGAATTCCCT CGCAAAGATC CAGCGGGAGA AAGAGACGAC GGACGCCAAG 
CTTCGGGACT CAGCGGCAGA GCTGGAGAAA TTGCACGAGA AGCTGAAGAG GCTGGAAAGG 
GAGCGAAAAG CCGATGCCGA GGAGTGGGGC ATGCTGCGGG CATCCCTGAC CGAGAAGCTG 
CAAGCTGCGG AGGAGGCGGC GTTGCGGAGG GTAGAAGAGC AACGGCGGCT GTTCAAGGAA 
GAGATGGACA AACACCGGCA GGAGAAGGCT GGCCAATTGC GGCAGCTCGA GGAAAGCCAC 
GCAGCAGAAC TGAAGCGCCA GGAAGAGCTG CTGAGCGAGA CAGAAACCAA GTGGCACAGC 
GAGGTGGCCC TGCACCAGCA GAGGCTGGAA GACGTGAGGG TGGAGCTCCA GGAAAAGCTA 
GCTTCTTCCG AGCAGCTCAG AGCCGAGATG CAGTCTATGG CGGAGGACGA GCGCAGGGAG 
CTGCTGAACT CTCTCGGCCG CTGCAAGGAG GAAGTGTCCG TGCTCCGGCG AAACCTGGTG 
GCATTCAGGG ATGAAGCAGG CTCCAAGATG GCCGCCGAGC AGGCCAAGGT GGCGTCTGCT 
CTGAAGGACG TCGACACGCT TCGCGTGGAA CTGGAGCAGA CTAGGACGGC GTTGCGTTTG 
AAAGAGGCAC AGGAAAAGTC ACGTGAGTCG CAGTTTCACA AGGAACTCAG CCGGGTGACC 
GAAGAAAGGG AGCGTGCCAT TCAGCGGCTG AACGAGGTCG ATGCCAGTCA TGCGGCTCTT 
CAGCGCGAGC TGTCTGAAAC TCTTGGCAAG AAATCCGAAG GGGAGCGTCG AGTCGAAGTT 
CTGACTCACC AGTTGTCCGA GGCACGTCAG AGTTACACGT TGGTCACGGC AGAGACCGAG 
CAGCTTCGCC GAGTCATTGG GGAGCTGCAG CAGGAGTTGC GCAGCGCCAC TCAGGAGAGG 
GACGCCTCGC TTCAGGAGGC TTCCGAAAAA TTCCGCGGCG CGGAGAAAGC GCGGGCGACG 
TTGGCGGAGG AGCTTCGCGA GCTCCGGAAC ACGCACGGCG GCGTAGAGCG GGCTCGGCAG 
GTCTTGGAGC GCAAGGTGCG AGCCCTGGAG TCGGCTTTGC TGGAGCACGG CTCGGAGCTG 
GACTCGCTCC GGAAGCTGTC CGAGCAGAGG GGACATCAGG TGGAATCGGC ACGTCAGGAC 
GTTCTCTCCT GGAAGTGGAA GGTTGCCGTG CTCGAGGAAG AGCGGGACGC GTGGCAGCGC 
GAGCGTGCCT CCTTGGGCCG TCGTCTGGAC GAGAGCGAGC GCCGGGCACA GGCGGAGCGC 
AACGCTCGTG AAGGGGCGGT GCGCGAGGCC GGCCGGGTTG CCGAGAGACT GGCCCAGGAC 
CGGGCCGACC TGGAGAAGCA GCTGCTGGCA TCCGGGCAAG CAACGCACGA GGCACGCCTG 
GCACTGTGCG CCGCGCAGGG CTCCACGGCT GCCCTCGAGA GCGAGCTCAA GGTCGTCTCC 
GAGTCTCGGA AGTCGATCGA GGGCACTCTG GACTCGCTGC TGAGCGTGCT GCGCTGCTCC 
CTGGGGATCC AGACCAAATA CGAGCTGCGG TCCCCCTCCC CCCAGCGACC GGCGGCGGGC 
CGGCGCCACG GCGGCGATCC GTCCAACGAG AGCTTTCGGG GGGACAGCTC GCCCTTTCGG 
TCGGAGGACT CCCTCGGCCG CTCCCTGCTG CGGCGGCCCA CCCCCGACGT CGAGGCCGAG 
CTCGTGCTAC AAGCCGTGGA CGCCCTGTCG CACCGCATCG CCACGCTTGA AGCCGACAAG 
GAGAAGGTGG AGGCTGAGTG CGAGCGTTCC CAGCGGGTGG TTGCCGGACT GGAGGACGAG 
CTCACGGCCA CAAAGTCCGC GCTTGCGCGA CTGACCAAGG CAAACGATGC TCTCGTTGCA 
GACAATGCGG AGCTGCGCTC GCAAGTGTCC TCTCAGCTGG AGGCGGTCTC GGAGCTCAAA 
CACACGTCCC AGACCCTGGA GTGGGAGAAA GCGTCCCTCG TGGACCAGCT GGCAGACGCA 
GCGCGCCGCT ACCAGCTCAA GACTGACCAG TGCGACAAAC TTCAGGCCGC CCTGAGTCGC 
AGCCGCGACC ACCCACCATC CCCGATTCGG CCCTCCGAGC CCCTCGAGTC TCGGCTGTCG 
AAGCTGGAGC TCGAGCGACA ACTCTCGCAA GCCAAGGTCG AGAGGCTCAA GCTCAGGGTG 
ACCGAGAGCC AGGCCGAAGT GAGGAGGCTG CAGGAAGCCA AGGAGGAAGC CACTTCCCAG 
CTGACTCGGC TCGAGAGGAG GGCGGAGAGC CTCGACCACG GCAACAGCCG CCTGAGGGCA 
GAGATTGCGG AGCATGCGTC CAAGGTGAAG CTCCTGAACG CTCACCTGGT GGAAGAGATT 
GCCAAGGGAC GCGGCCTGGA GGAGGAGAAG CGATCTCTGG AAAGCCAGCT CAGGGTGGCC 
AGGGTGCAGC TCGAGTCTGC CAGGTTCGAC CGGAAAAGAG ATGGGTCGGT GATTGACGAG 
CTGAAGGGCC AGAAGGGCAC TCTGGAGACC AGGCTGTCTG CGCTCGCCGT ACAGCTGGCA 
GAATGCGAGT CAGCCAAGCA GCGCCTCGAC AAGACGCCCA TCAGGACTCC CCACAGGGAA 
GAGACCAGGA CTTGGCAGCA GATGGTGGAG ACGTTGCGCG AGGAGAAGCG GCAGCTCCAG 
GGGGAGGTGA CGCGCCTCCG GCGGCAGCTC ACGGGCACCA TTGACAGTAT CCTGCCGCCG 
GCGACTCCCG ACGGGAGCCG AGCTTCGAGG CGGACACGCC GCAGTCTGGC AGAGCGTGAC 
GTGCCAGACG GCGCAAGCCT CCATTCTCCC GAGGTGTCTC GCCTGAAGAC GCAGAAGAGC 
CTCCTGGAGG CCAAAGTCCG CACTTTGGAG GAGCGCCTCT CTCAGCAAGA CCAGGTGCAC 
TCGGAGGAGC TGACGCGGGA ACACCGGGAG CTGGTCGCCG AGATCGCCAG ACTGCGGGAG 
GAGCTGGCAA CGCACGAGCG GCGCCGGACG TCGACCCCCG TGCACAACGA GCCTCCCGAG 
TTTGCCATCA ATCTAAGCCA CAGGCAAGAC ATCCAAGGGC TGCTCGACCG TTCCCTCCAC 
ACCGTGTCCG AAGACCCGGA GCGGCTGCGC GACCAAGCCA TGTTGCTCAG CGCGTTCATC 
GACGGAGCAC TGGAACCGGG GGACAGCCTT CGGACCCCTT CTAAGCTTGC GCCAAACAAG 
CGGACCACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029851191.1
CDS	110..5281
Translation	MTYLGDGQVQAVQALGGSFQHSGGAVFESPLRIAVMEQPPENVIARLPSSSTINDEDYATAEEEPPGRTSRLEDDDQQGVLSDACSTDSDHELELVGLDREHELKSKLEKSRTMLRAYVQHTTNQDRLLGHLGAQVEEYRNRCSRLEEVLRQCPCADRTENIQDDRSYEEDPPDLPSALSALELERARCAELKQLNVVLREHLEITTETNATLTMHMERMTEDWQNATRADAVNRGSSSSATHDEFRADSEQGSVPRLRRDLQELKGDLGDMRQAFATSLASIQTMLHDAAEAAGRVQLSLVDDAADSDQPDLGLTRSRDEGVAGVSAAPLDSVAKMVTDDLNHQVEALHRENEGLHTVLASRLETIEQMTNVIDALKAAQCNLGAISEKSSQENVDLRVREAMLQSEVRSHEATSKNLALELESVQKEKKECMGVIASLRKHVANLEDQQQVSERELSVLRKAVRDIEELNDSLEREMQEMCNASMQLKQSNSELAKAKSDMELAHEELAAERDSLARREADVTRAMARLESLLEESASRQTDLEATLSARDKEIAYRKAAFLQLSSQKDALSQEASSLRQSVASQRETNEQLSAQKEELATAKSQLEMELRHCRAEGDKLQSDLTGCKEAREATEVDLRNARNSLAKIQREKETTDAKLRDSAAELEKLHEKLKRLERERKADAEEWGMLRASLTEKLQAAEEAALRRVEEQRRLFKEEMDKHRQEKAGQLRQLEESHAAELKRQEELLSETETKWHSEVALHQQRLEDVRVELQEKLASSEQLRAEMQSMAEDERRELLNSLGRCKEEVSVLRRNLVAFRDEAGSKMAAEQAKVASALKDVDTLRVELEQTRTALRLKEAQEKSRESQFHKELSRVTEERERAIQRLNEVDASHAALQRELSETLGKKSEGERRVEVLTHQLSEARQSYTLVTAETEQLRRVIGELQQELRSATQERDASLQEASEKFRGAEKARATLAEELRELRNTHGGVERARQVLERKVRALESALLEHGSELDSLRKLSEQRGHQVESARQDVLSWKWKVAVLEEERDAWQRERASLGRRLDESERRAQAERNAREGAVREAGRVAERLAQDRADLEKQLLASGQATHEARLALCAAQGSTAALESELKVVSESRKSIEGTLDSLLSVLRCSLGIQTKYELRSPSPQRPAAGRRHGGDPSNESFRGDSSPFRSEDSLGRSLLRRPTPDVEAELVLQAVDALSHRIATLEADKEKVEAECERSQRVVAGLEDELTATKSALARLTKANDALVADNAELRSQVSSQLEAVSELKHTSQTLEWEKASLVDQLADAARRYQLKTDQCDKLQAALSRSRDHPPSPIRPSEPLESRLSKLELERQLSQAKVERLKLRVTESQAEVRRLQEAKEEATSQLTRLERRAESLDHGNSRLRAEIAEHASKVKLLNAHLVEEIAKGRGLEEEKRSLESQLRVARVQLESARFDRKRDGSVIDELKGQKGTLETRLSALAVQLAECESAKQRLDKTPIRTPHREETRTWQQMVETLREEKRQLQGEVTRLRRQLTGTIDSILPPATPDGSRASRRTRRSLAERDVPDGASLHSPEVSRLKTQKSLLEAKVRTLEERLSQQDQVHSEELTREHRELVAEIARLREELATHERRRTSTPVHNEPPEFAINLSHRQDIQGLLDRSLHTVSEDPERLRDQAMLLSAFIDGALEPGDSLRTPSKLAPNKRTT

Target ORF information:

RefSeq Version	XM_029995331.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis rootletin (LOC8029650), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029995331.1

ORF Insert Sequence:

ATGACATATC TTGGCGACGG GCAAGTTCAG GCGGTTCAGG CACTCGGTGG TTCATTCCAA 
CATTCTGGTG GAGCGGTATT TGAAAGCCCG CTTCGCATTG CAGTGATGGA GCAGCCACCG 
GAGAACGTAA TTGCGAGGCT ACCGTCTTCG TCGACCATCA ATGACGAGGA CTATGCAACG 
GCAGAGGAGG AACCACCAGG CAGAACCTCG AGGCTCGAGG ACGACGACCA GCAGGGCGTG 
CTCTCGGACG CCTGCTCGAC CGACAGCGAC CACGAGCTCG AGTTGGTGGG TCTGGATCGC 
GAACACGAGC TCAAGTCGAA GCTGGAGAAG TCGAGGACCA TGTTGCGGGC CTACGTGCAG 
CACACGACCA ATCAGGACAG GCTGCTTGGG CACCTGGGAG CACAGGTGGA GGAGTACCGG 
AACCGCTGCT CGAGATTGGA AGAGGTCCTC CGCCAGTGCC CGTGTGCCGA TCGAACTGAG 
AACATTCAAG ACGATCGCTC GTACGAAGAA GATCCTCCTG ACCTTCCGTC GGCCTTATCA 
GCGCTGGAAC TAGAGAGGGC AAGATGTGCG GAGCTGAAGC AGCTGAACGT GGTCCTCCGC 
GAGCACCTGG AGATAACGAC GGAGACGAAT GCGACCCTCA CCATGCACAT GGAGAGGATG 
ACGGAGGACT GGCAGAACGC GACCCGGGCT GACGCGGTCA ATCGCGGCAG CAGCAGCAGC 
GCCACCCACG ACGAGTTTCG CGCAGACTCC GAGCAGGGCA GCGTTCCCAG GCTGCGTCGA 
GACTTGCAGG AGCTGAAGGG GGACCTGGGA GACATGAGGC AGGCCTTTGC CACCAGCCTG 
GCCTCCATCC AGACGATGCT GCACGACGCC GCCGAGGCGG CCGGGCGTGT CCAACTCTCG 
CTTGTGGACG ACGCGGCGGA CAGCGACCAG CCAGACCTCG GGCTCACCAG GTCTCGGGAC 
GAGGGCGTGG CGGGAGTCTC AGCGGCGCCG CTGGACTCTG TCGCCAAAAT GGTGACAGAC 
GACCTCAACC ACCAAGTGGA GGCGTTGCAT CGTGAAAACG AGGGCCTGCA CACGGTCCTG 
GCGAGCAGGC TGGAGACCAT CGAGCAGATG ACAAACGTCA TCGATGCGCT TAAGGCTGCG 
CAGTGTAACC TCGGAGCCAT ATCTGAGAAG AGCAGCCAGG AAAACGTGGA CCTGCGCGTG 
CGGGAAGCCA TGCTACAGTC GGAGGTGCGC AGCCACGAGG CCACGTCGAA GAATCTCGCC 
TTGGAGCTGG AGTCCGTCCA AAAGGAAAAG AAAGAGTGCA TGGGTGTAAT TGCGTCGCTG 
CGCAAGCATG TCGCCAACCT CGAGGACCAG CAACAGGTGT CCGAACGCGA GCTCAGCGTT 
CTGAGGAAAG CGGTGCGTGA CATCGAAGAA CTCAACGACT CCCTGGAACG GGAGATGCAA 
GAGATGTGCA ACGCCAGCAT GCAGCTTAAA CAGTCCAACT CGGAGCTGGC AAAGGCAAAG 
AGTGACATGG AACTTGCGCA CGAAGAGTTG GCCGCGGAAC GGGACTCCCT GGCACGACGG 
GAAGCGGATG TAACGCGAGC GATGGCCCGT CTGGAGTCGC TCTTGGAAGA GAGCGCGTCT 
CGGCAGACGG ATCTGGAGGC CACGCTCTCT GCCAGGGACA AGGAGATCGC CTACAGGAAG 
GCGGCGTTTC TTCAGCTGTC CTCTCAGAAG GACGCGCTCT CGCAAGAAGC GTCCAGCCTT 
CGGCAGAGTG TCGCGAGCCA GCGGGAGACA AACGAGCAGC TCAGCGCACA GAAGGAAGAG 
CTGGCCACGG CCAAGTCTCA GCTGGAAATG GAGCTACGGC ACTGCCGGGC CGAGGGGGAC 
AAGCTCCAGA GCGACCTGAC TGGCTGCAAG GAAGCCCGGG AAGCGACAGA AGTGGACCTG 
CGGAACGCCA GGAATTCCCT CGCAAAGATC CAGCGGGAGA AAGAGACGAC GGACGCCAAG 
CTTCGGGACT CAGCGGCAGA GCTGGAGAAA TTGCACGAGA AGCTGAAGAG GCTGGAAAGG 
GAGCGAAAAG CCGATGCCGA GGAGTGGGGC ATGCTGCGGG CATCCCTGAC CGAGAAGCTG 
CAAGCTGCGG AGGAGGCGGC GTTGCGGAGG GTAGAAGAGC AACGGCGGCT GTTCAAGGAA 
GAGATGGACA AACACCGGCA GGAGAAGGCT GGCCAATTGC GGCAGCTCGA GGAAAGCCAC 
GCAGCAGAAC TGAAGCGCCA GGAAGAGCTG CTGAGCGAGA CAGAAACCAA GTGGCACAGC 
GAGGTGGCCC TGCACCAGCA GAGGCTGGAA GACGTGAGGG TGGAGCTCCA GGAAAAGCTA 
GCTTCTTCCG AGCAGCTCAG AGCCGAGATG CAGTCTATGG CGGAGGACGA GCGCAGGGAG 
CTGCTGAACT CTCTCGGCCG CTGCAAGGAG GAAGTGTCCG TGCTCCGGCG AAACCTGGTG 
GCATTCAGGG ATGAAGCAGG CTCCAAGATG GCCGCCGAGC AGGCCAAGGT GGCGTCTGCT 
CTGAAGGACG TCGACACGCT TCGCGTGGAA CTGGAGCAGA CTAGGACGGC GTTGCGTTTG 
AAAGAGGCAC AGGAAAAGTC ACGTGAGTCG CAGTTTCACA AGGAACTCAG CCGGGTGACC 
GAAGAAAGGG AGCGTGCCAT TCAGCGGCTG AACGAGGTCG ATGCCAGTCA TGCGGCTCTT 
CAGCGCGAGC TGTCTGAAAC TCTTGGCAAG AAATCCGAAG GGGAGCGTCG AGTCGAAGTT 
CTGACTCACC AGTTGTCCGA GGCACGTCAG AGTTACACGT TGGTCACGGC AGAGACCGAG 
CAGCTTCGCC GAGTCATTGG GGAGCTGCAG CAGGAGTTGC GCAGCGCCAC TCAGGAGAGG 
GACGCCTCGC TTCAGGAGGC TTCCGAAAAA TTCCGCGGCG CGGAGAAAGC GCGGGCGACG 
TTGGCGGAGG AGCTTCGCGA GCTCCGGAAC ACGCACGGCG GCGTAGAGCG GGCTCGGCAG 
GTCTTGGAGC GCAAGGTGCG AGCCCTGGAG TCGGCTTTGC TGGAGCACGG CTCGGAGCTG 
GACTCGCTCC GGAAGCTGTC CGAGCAGAGG GGACATCAGG TGGAATCGGC ACGTCAGGAC 
GTTCTCTCCT GGAAGTGGAA GGTTGCCGTG CTCGAGGAAG AGCGGGACGC GTGGCAGCGC 
GAGCGTGCCT CCTTGGGCCG TCGTCTGGAC GAGAGCGAGC GCCGGGCACA GGCGGAGCGC 
AACGCTCGTG AAGGGGCGGT GCGCGAGGCC GGCCGGGTTG CCGAGAGACT GGCCCAGGAC 
CGGGCCGACC TGGAGAAGCA GCTGCTGGCA TCCGGGCAAG CAACGCACGA GGCACGCCTG 
GCACTGTGCG CCGCGCAGGG CTCCACGGCT GCCCTCGAGA GCGAGCTCAA GGTCGTCTCC 
GAGTCTCGGA AGTCGATCGA GGGCACTCTG GACTCGCTGC TGAGCGTGCT GCGCTGCTCC 
CTGGGGATCC AGACCAAATA CGAGCTGCGG TCCCCCTCCC CCCAGCGACC GGCGGCGGGC 
CGGCGCCACG GCGGCGATCC GTCCAACGAG AGCTTTCGGG GGGACAGCTC GCCCTTTCGG 
TCGGAGGACT CCCTCGGCCG CTCCCTGCTG CGGCGGCCCA CCCCCGACGT CGAGGCCGAG 
CTCGTGCTAC AAGCCGTGGA CGCCCTGTCG CACCGCATCG CCACGCTTGA AGCCGACAAG 
GAGAAGGTGG AGGCTGAGTG CGAGCGTTCC CAGCGGGTGG TTGCCGGACT GGAGGACGAG 
CTCACGGCCA CAAAGTCCGC GCTTGCGCGA CTGACCAAGG CAAACGATGC TCTCGTTGCA 
GACAATGCGG AGCTGCGCTC GCAAGTGTCC TCTCAGCTGG AGGCGGTCTC GGAGCTCAAA 
CACACGTCCC AGACCCTGGA GTGGGAGAAA GCGTCCCTCG TGGACCAGCT GGCAGACGCA 
GCGCGCCGCT ACCAGCTCAA GACTGACCAG TGCGACAAAC TTCAGGCCGC CCTGAGTCGC 
AGCCGCGACC ACCCACCATC CCCGATTCGG CCCTCCGAGC CCCTCGAGTC TCGGCTGTCG 
AAGCTGGAGC TCGAGCGACA ACTCTCGCAA GCCAAGGTCG AGAGGCTCAA GCTCAGGGTG 
ACCGAGAGCC AGGCCGAAGT GAGGAGGCTG CAGGAAGCCA AGGAGGAAGC CACTTCCCAG 
CTGACTCGGC TCGAGAGGAG GGCGGAGAGC CTCGACCACG GCAACAGCCG CCTGAGGGCA 
GAGATTGCGG AGCATGCGTC CAAGGTGAAG CTCCTGAACG CTCACCTGGT GGAAGAGATT 
GCCAAGGGAC GCGGCCTGGA GGAGGAGAAG CGATCTCTGG AAAGCCAGCT CAGGGTGGCC 
AGGGTGCAGC TCGAGTCTGC CAGGTTCGAC CGGAAAAGAG ATGGGTCGGT GATTGACGAG 
CTGAAGGGCC AGAAGGGCAC TCTGGAGACC AGGCTGTCTG CGCTCGCCGT ACAGCTGGCA 
GAATGCGAGT CAGCCAAGCA GCGCCTCGAC AAGACGCCCA TCAGGACTCC CCACAGGGAA 
GAGACCAGGA CTTGGCAGCA GATGGTGGAG ACGTTGCGCG AGGAGAAGCG GCAGCTCCAG 
GGGGAGGTGA CGCGCCTCCG GCGGCAGCTC ACGGGCACCA TTGACAGTAT CCTGCCGCCG 
GCGACTCCCG ACGGGAGCCG AGCTTCGAGG CGGACACGCC GCAGTCTGGC AGAGCGTGAC 
GTGCCAGACG GCGCAAGCCT CCATTCTCCC GAGGTGTCTC GCCTGAAGAC GCAGAAGAGC 
CTCCTGGAGG CCAAAGTCCG CACTTTGGAG GAGCGCCTCT CTCAGCAAGA CCAGGTGCAC 
TCGGAGGAGC TGACGCGGGA ACACCGGGAG CTGGTCGCCG AGATCGCCAG ACTGCGGGAG 
GAGCTGGCAA CGCACGAGCG GCGCCGGACG TCGACCCCCG TGCACAACGA GCCTCCCGAG 
TTTGCCATCA ATCTAAGCCA CAGGCAAGAC ATCCAAGGGC TGCTCGACCG TTCCCTCCAC 
ACCGTGTCCG AAGACCCGGA GCGGCTGCGC GACCAAGCCA TGTTGCTCAG CGCGTTCATC 
GACGGAGCAC TGGAACCGGG GGACAGCCTT CGGACCCCTT CTAAGCTTGC GCCAAACAAG 
CGGACCACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8029650 ( XM_029995332.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029851192.1 Ixodes scapularis rootletin (LOC8029650), transcript variant X2, mRNA.

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CloneID	OIa36135
Clone ID Related Accession (Same CDS sequence)	XM_029995332.1
Accession Version	XM_029995332.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	rootletin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021922528.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATATC TTGGCGACGG GCAAGTTCAG GCGGTTCAGG CACTCGGTGG TTCATTCCAA 
CATTCTGGTG GAGCGGTATT TGAAAGCCCG CTTCGCATTG CAGTGATGGA GCAGCCACCG 
GAGAACGTAA TTGCGAGGCT ACCGTCTTCG TCGACCATCA ATGACGAGGA CTATGCAACG 
GCAGAGGAGG AACCACCAGG CAGAACCTCG AGGCTCGAGG ACGACGACCA GCAGGGCGTG 
CTCTCGGACG CCTGCTCGAC CGACAGCGAC CACGAGCTCG AGTTGGTGGG TCTGGATCGC 
GAACACGAGC TCAAGTCGAA GCTGGAGAAG TCGAGGACCA TGTTGCGGGC CTACGTGCAG 
CACACGACCA ATCAGGACAG GCTGCTTGGG CACCTGGGAG CACAGGTGGA GGAGTACCGG 
AACCGCTGCT CGAGATTGGA AGAGGTCCTC CGCCAGTGCC CGTGTGCCGA TCGAACTGAG 
AACATTCAAG ACGATCGCTC GTACGAAGAA GATCCTCCTG ACCTTCCGTC GGCCTTATCA 
GCGCTGGAAC TAGAGAGGGC AAGATGTGCG GAGCTGAAGC AGCTGAACGT GGTCCTCCGC 
GAGCACCTGG AGATAACGAC GGAGACGAAT GCGACCCTCA CCATGCACAT GGAGAGGATG 
ACGGAGGACT GGCAGAACGC GACCCGGGCT GACGCGGTCA ATCGCGGCAG CAGCAGCAGC 
GCCACCCACG ACGAGTTTCG CGCAGACTCC GAGCAGGGCA GCGTTCCCAG GCTGCGTCGA 
GACTTGCAGG AGCTGAAGGG GGACCTGGGA GACATGAGGC AGGCCTTTGC CACCAGCCTG 
GCCTCCATCC AGACGATGCT GCACGACGCC GCCGAGGCGG CCGGGCGTGT CCAACTCTCG 
CTTGTGGACG ACGCGGCGGA CAGCGACCAG CCAGACCTCG GGCTCACCAG GTCTCGGGAC 
GAGGGCGTGG CGGGAGTCTC AGCGGCGCCG CTGGACTCTG TCGCCAAAAT GGTGACAGAC 
GACCTCAACC ACCAAGTGGA GGCGTTGCAT CGTGAAAACG AGGGCCTGCA CACGGTCCTG 
GCGAGCAGGC TGGAGACCAT CGAGCAGATG ACAAACGTCA TCGATGCGCT TAAGGCTGCG 
CAGTGTAACC TCGGAGCCAT ATCTGAGAAG AGCCAGGAAA ACGTGGACCT GCGCGTGCGG 
GAAGCCATGC TACAGTCGGA GGTGCGCAGC CACGAGGCCA CGTCGAAGAA TCTCGCCTTG 
GAGCTGGAGT CCGTCCAAAA GGAAAAGAAA GAGTGCATGG GTGTAATTGC GTCGCTGCGC 
AAGCATGTCG CCAACCTCGA GGACCAGCAA CAGGTGTCCG AACGCGAGCT CAGCGTTCTG 
AGGAAAGCGG TGCGTGACAT CGAAGAACTC AACGACTCCC TGGAACGGGA GATGCAAGAG 
ATGTGCAACG CCAGCATGCA GCTTAAACAG TCCAACTCGG AGCTGGCAAA GGCAAAGAGT 
GACATGGAAC TTGCGCACGA AGAGTTGGCC GCGGAACGGG ACTCCCTGGC ACGACGGGAA 
GCGGATGTAA CGCGAGCGAT GGCCCGTCTG GAGTCGCTCT TGGAAGAGAG CGCGTCTCGG 
CAGACGGATC TGGAGGCCAC GCTCTCTGCC AGGGACAAGG AGATCGCCTA CAGGAAGGCG 
GCGTTTCTTC AGCTGTCCTC TCAGAAGGAC GCGCTCTCGC AAGAAGCGTC CAGCCTTCGG 
CAGAGTGTCG CGAGCCAGCG GGAGACAAAC GAGCAGCTCA GCGCACAGAA GGAAGAGCTG 
GCCACGGCCA AGTCTCAGCT GGAAATGGAG CTACGGCACT GCCGGGCCGA GGGGGACAAG 
CTCCAGAGCG ACCTGACTGG CTGCAAGGAA GCCCGGGAAG CGACAGAAGT GGACCTGCGG 
AACGCCAGGA ATTCCCTCGC AAAGATCCAG CGGGAGAAAG AGACGACGGA CGCCAAGCTT 
CGGGACTCAG CGGCAGAGCT GGAGAAATTG CACGAGAAGC TGAAGAGGCT GGAAAGGGAG 
CGAAAAGCCG ATGCCGAGGA GTGGGGCATG CTGCGGGCAT CCCTGACCGA GAAGCTGCAA 
GCTGCGGAGG AGGCGGCGTT GCGGAGGGTA GAAGAGCAAC GGCGGCTGTT CAAGGAAGAG 
ATGGACAAAC ACCGGCAGGA GAAGGCTGGC CAATTGCGGC AGCTCGAGGA AAGCCACGCA 
GCAGAACTGA AGCGCCAGGA AGAGCTGCTG AGCGAGACAG AAACCAAGTG GCACAGCGAG 
GTGGCCCTGC ACCAGCAGAG GCTGGAAGAC GTGAGGGTGG AGCTCCAGGA AAAGCTAGCT 
TCTTCCGAGC AGCTCAGAGC CGAGATGCAG TCTATGGCGG AGGACGAGCG CAGGGAGCTG 
CTGAACTCTC TCGGCCGCTG CAAGGAGGAA GTGTCCGTGC TCCGGCGAAA CCTGGTGGCA 
TTCAGGGATG AAGCAGGCTC CAAGATGGCC GCCGAGCAGG CCAAGGTGGC GTCTGCTCTG 
AAGGACGTCG ACACGCTTCG CGTGGAACTG GAGCAGACTA GGACGGCGTT GCGTTTGAAA 
GAGGCACAGG AAAAGTCACG TGAGTCGCAG TTTCACAAGG AACTCAGCCG GGTGACCGAA 
GAAAGGGAGC GTGCCATTCA GCGGCTGAAC GAGGTCGATG CCAGTCATGC GGCTCTTCAG 
CGCGAGCTGT CTGAAACTCT TGGCAAGAAA TCCGAAGGGG AGCGTCGAGT CGAAGTTCTG 
ACTCACCAGT TGTCCGAGGC ACGTCAGAGT TACACGTTGG TCACGGCAGA GACCGAGCAG 
CTTCGCCGAG TCATTGGGGA GCTGCAGCAG GAGTTGCGCA GCGCCACTCA GGAGAGGGAC 
GCCTCGCTTC AGGAGGCTTC CGAAAAATTC CGCGGCGCGG AGAAAGCGCG GGCGACGTTG 
GCGGAGGAGC TTCGCGAGCT CCGGAACACG CACGGCGGCG TAGAGCGGGC TCGGCAGGTC 
TTGGAGCGCA AGGTGCGAGC CCTGGAGTCG GCTTTGCTGG AGCACGGCTC GGAGCTGGAC 
TCGCTCCGGA AGCTGTCCGA GCAGAGGGGA CATCAGGTGG AATCGGCACG TCAGGACGTT 
CTCTCCTGGA AGTGGAAGGT TGCCGTGCTC GAGGAAGAGC GGGACGCGTG GCAGCGCGAG 
CGTGCCTCCT TGGGCCGTCG TCTGGACGAG AGCGAGCGCC GGGCACAGGC GGAGCGCAAC 
GCTCGTGAAG GGGCGGTGCG CGAGGCCGGC CGGGTTGCCG AGAGACTGGC CCAGGACCGG 
GCCGACCTGG AGAAGCAGCT GCTGGCATCC GGGCAAGCAA CGCACGAGGC ACGCCTGGCA 
CTGTGCGCCG CGCAGGGCTC CACGGCTGCC CTCGAGAGCG AGCTCAAGGT CGTCTCCGAG 
TCTCGGAAGT CGATCGAGGG CACTCTGGAC TCGCTGCTGA GCGTGCTGCG CTGCTCCCTG 
GGGATCCAGA CCAAATACGA GCTGCGGTCC CCCTCCCCCC AGCGACCGGC GGCGGGCCGG 
CGCCACGGCG GCGATCCGTC CAACGAGAGC TTTCGGGGGG ACAGCTCGCC CTTTCGGTCG 
GAGGACTCCC TCGGCCGCTC CCTGCTGCGG CGGCCCACCC CCGACGTCGA GGCCGAGCTC 
GTGCTACAAG CCGTGGACGC CCTGTCGCAC CGCATCGCCA CGCTTGAAGC CGACAAGGAG 
AAGGTGGAGG CTGAGTGCGA GCGTTCCCAG CGGGTGGTTG CCGGACTGGA GGACGAGCTC 
ACGGCCACAA AGTCCGCGCT TGCGCGACTG ACCAAGGCAA ACGATGCTCT CGTTGCAGAC 
AATGCGGAGC TGCGCTCGCA AGTGTCCTCT CAGCTGGAGG CGGTCTCGGA GCTCAAACAC 
ACGTCCCAGA CCCTGGAGTG GGAGAAAGCG TCCCTCGTGG ACCAGCTGGC AGACGCAGCG 
CGCCGCTACC AGCTCAAGAC TGACCAGTGC GACAAACTTC AGGCCGCCCT GAGTCGCAGC 
CGCGACCACC CACCATCCCC GATTCGGCCC TCCGAGCCCC TCGAGTCTCG GCTGTCGAAG 
CTGGAGCTCG AGCGACAACT CTCGCAAGCC AAGGTCGAGA GGCTCAAGCT CAGGGTGACC 
GAGAGCCAGG CCGAAGTGAG GAGGCTGCAG GAAGCCAAGG AGGAAGCCAC TTCCCAGCTG 
ACTCGGCTCG AGAGGAGGGC GGAGAGCCTC GACCACGGCA ACAGCCGCCT GAGGGCAGAG 
ATTGCGGAGC ATGCGTCCAA GGTGAAGCTC CTGAACGCTC ACCTGGTGGA AGAGATTGCC 
AAGGGACGCG GCCTGGAGGA GGAGAAGCGA TCTCTGGAAA GCCAGCTCAG GGTGGCCAGG 
GTGCAGCTCG AGTCTGCCAG GTTCGACCGG AAAAGAGATG GGTCGGTGAT TGACGAGCTG 
AAGGGCCAGA AGGGCACTCT GGAGACCAGG CTGTCTGCGC TCGCCGTACA GCTGGCAGAA 
TGCGAGTCAG CCAAGCAGCG CCTCGACAAG ACGCCCATCA GGACTCCCCA CAGGGAAGAG 
ACCAGGACTT GGCAGCAGAT GGTGGAGACG TTGCGCGAGG AGAAGCGGCA GCTCCAGGGG 
GAGGTGACGC GCCTCCGGCG GCAGCTCACG GGCACCATTG ACAGTATCCT GCCGCCGGCG 
ACTCCCGACG GGAGCCGAGC TTCGAGGCGG ACACGCCGCA GTCTGGCAGA GCGTGACGTG 
CCAGACGGCG CAAGCCTCCA TTCTCCCGAG GTGTCTCGCC TGAAGACGCA GAAGAGCCTC 
CTGGAGGCCA AAGTCCGCAC TTTGGAGGAG CGCCTCTCTC AGCAAGACCA GGTGCACTCG 
GAGGAGCTGA CGCGGGAACA CCGGGAGCTG GTCGCCGAGA TCGCCAGACT GCGGGAGGAG 
CTGGCAACGC ACGAGCGGCG CCGGACGTCG ACCCCCGTGC ACAACGAGCC TCCCGAGTTT 
GCCATCAATC TAAGCCACAG GCAAGACATC CAAGGGCTGC TCGACCGTTC CCTCCACACC 
GTGTCCGAAG ACCCGGAGCG GCTGCGCGAC CAAGCCATGT TGCTCAGCGC GTTCATCGAC 
GGAGCACTGG AACCGGGGGA CAGCCTTCGG ACCCCTTCTA AGCTTGCGCC AAACAAGCGG 
ACCACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029851192.1
CDS	110..5278
Translation	MTYLGDGQVQAVQALGGSFQHSGGAVFESPLRIAVMEQPPENVIARLPSSSTINDEDYATAEEEPPGRTSRLEDDDQQGVLSDACSTDSDHELELVGLDREHELKSKLEKSRTMLRAYVQHTTNQDRLLGHLGAQVEEYRNRCSRLEEVLRQCPCADRTENIQDDRSYEEDPPDLPSALSALELERARCAELKQLNVVLREHLEITTETNATLTMHMERMTEDWQNATRADAVNRGSSSSATHDEFRADSEQGSVPRLRRDLQELKGDLGDMRQAFATSLASIQTMLHDAAEAAGRVQLSLVDDAADSDQPDLGLTRSRDEGVAGVSAAPLDSVAKMVTDDLNHQVEALHRENEGLHTVLASRLETIEQMTNVIDALKAAQCNLGAISEKSQENVDLRVREAMLQSEVRSHEATSKNLALELESVQKEKKECMGVIASLRKHVANLEDQQQVSERELSVLRKAVRDIEELNDSLEREMQEMCNASMQLKQSNSELAKAKSDMELAHEELAAERDSLARREADVTRAMARLESLLEESASRQTDLEATLSARDKEIAYRKAAFLQLSSQKDALSQEASSLRQSVASQRETNEQLSAQKEELATAKSQLEMELRHCRAEGDKLQSDLTGCKEAREATEVDLRNARNSLAKIQREKETTDAKLRDSAAELEKLHEKLKRLERERKADAEEWGMLRASLTEKLQAAEEAALRRVEEQRRLFKEEMDKHRQEKAGQLRQLEESHAAELKRQEELLSETETKWHSEVALHQQRLEDVRVELQEKLASSEQLRAEMQSMAEDERRELLNSLGRCKEEVSVLRRNLVAFRDEAGSKMAAEQAKVASALKDVDTLRVELEQTRTALRLKEAQEKSRESQFHKELSRVTEERERAIQRLNEVDASHAALQRELSETLGKKSEGERRVEVLTHQLSEARQSYTLVTAETEQLRRVIGELQQELRSATQERDASLQEASEKFRGAEKARATLAEELRELRNTHGGVERARQVLERKVRALESALLEHGSELDSLRKLSEQRGHQVESARQDVLSWKWKVAVLEEERDAWQRERASLGRRLDESERRAQAERNAREGAVREAGRVAERLAQDRADLEKQLLASGQATHEARLALCAAQGSTAALESELKVVSESRKSIEGTLDSLLSVLRCSLGIQTKYELRSPSPQRPAAGRRHGGDPSNESFRGDSSPFRSEDSLGRSLLRRPTPDVEAELVLQAVDALSHRIATLEADKEKVEAECERSQRVVAGLEDELTATKSALARLTKANDALVADNAELRSQVSSQLEAVSELKHTSQTLEWEKASLVDQLADAARRYQLKTDQCDKLQAALSRSRDHPPSPIRPSEPLESRLSKLELERQLSQAKVERLKLRVTESQAEVRRLQEAKEEATSQLTRLERRAESLDHGNSRLRAEIAEHASKVKLLNAHLVEEIAKGRGLEEEKRSLESQLRVARVQLESARFDRKRDGSVIDELKGQKGTLETRLSALAVQLAECESAKQRLDKTPIRTPHREETRTWQQMVETLREEKRQLQGEVTRLRRQLTGTIDSILPPATPDGSRASRRTRRSLAERDVPDGASLHSPEVSRLKTQKSLLEAKVRTLEERLSQQDQVHSEELTREHRELVAEIARLREELATHERRRTSTPVHNEPPEFAINLSHRQDIQGLLDRSLHTVSEDPERLRDQAMLLSAFIDGALEPGDSLRTPSKLAPNKRTT

Target ORF information:

RefSeq Version	XM_029995332.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis rootletin (LOC8029650), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029995332.1

ORF Insert Sequence:

ATGACATATC TTGGCGACGG GCAAGTTCAG GCGGTTCAGG CACTCGGTGG TTCATTCCAA 
CATTCTGGTG GAGCGGTATT TGAAAGCCCG CTTCGCATTG CAGTGATGGA GCAGCCACCG 
GAGAACGTAA TTGCGAGGCT ACCGTCTTCG TCGACCATCA ATGACGAGGA CTATGCAACG 
GCAGAGGAGG AACCACCAGG CAGAACCTCG AGGCTCGAGG ACGACGACCA GCAGGGCGTG 
CTCTCGGACG CCTGCTCGAC CGACAGCGAC CACGAGCTCG AGTTGGTGGG TCTGGATCGC 
GAACACGAGC TCAAGTCGAA GCTGGAGAAG TCGAGGACCA TGTTGCGGGC CTACGTGCAG 
CACACGACCA ATCAGGACAG GCTGCTTGGG CACCTGGGAG CACAGGTGGA GGAGTACCGG 
AACCGCTGCT CGAGATTGGA AGAGGTCCTC CGCCAGTGCC CGTGTGCCGA TCGAACTGAG 
AACATTCAAG ACGATCGCTC GTACGAAGAA GATCCTCCTG ACCTTCCGTC GGCCTTATCA 
GCGCTGGAAC TAGAGAGGGC AAGATGTGCG GAGCTGAAGC AGCTGAACGT GGTCCTCCGC 
GAGCACCTGG AGATAACGAC GGAGACGAAT GCGACCCTCA CCATGCACAT GGAGAGGATG 
ACGGAGGACT GGCAGAACGC GACCCGGGCT GACGCGGTCA ATCGCGGCAG CAGCAGCAGC 
GCCACCCACG ACGAGTTTCG CGCAGACTCC GAGCAGGGCA GCGTTCCCAG GCTGCGTCGA 
GACTTGCAGG AGCTGAAGGG GGACCTGGGA GACATGAGGC AGGCCTTTGC CACCAGCCTG 
GCCTCCATCC AGACGATGCT GCACGACGCC GCCGAGGCGG CCGGGCGTGT CCAACTCTCG 
CTTGTGGACG ACGCGGCGGA CAGCGACCAG CCAGACCTCG GGCTCACCAG GTCTCGGGAC 
GAGGGCGTGG CGGGAGTCTC AGCGGCGCCG CTGGACTCTG TCGCCAAAAT GGTGACAGAC 
GACCTCAACC ACCAAGTGGA GGCGTTGCAT CGTGAAAACG AGGGCCTGCA CACGGTCCTG 
GCGAGCAGGC TGGAGACCAT CGAGCAGATG ACAAACGTCA TCGATGCGCT TAAGGCTGCG 
CAGTGTAACC TCGGAGCCAT ATCTGAGAAG AGCCAGGAAA ACGTGGACCT GCGCGTGCGG 
GAAGCCATGC TACAGTCGGA GGTGCGCAGC CACGAGGCCA CGTCGAAGAA TCTCGCCTTG 
GAGCTGGAGT CCGTCCAAAA GGAAAAGAAA GAGTGCATGG GTGTAATTGC GTCGCTGCGC 
AAGCATGTCG CCAACCTCGA GGACCAGCAA CAGGTGTCCG AACGCGAGCT CAGCGTTCTG 
AGGAAAGCGG TGCGTGACAT CGAAGAACTC AACGACTCCC TGGAACGGGA GATGCAAGAG 
ATGTGCAACG CCAGCATGCA GCTTAAACAG TCCAACTCGG AGCTGGCAAA GGCAAAGAGT 
GACATGGAAC TTGCGCACGA AGAGTTGGCC GCGGAACGGG ACTCCCTGGC ACGACGGGAA 
GCGGATGTAA CGCGAGCGAT GGCCCGTCTG GAGTCGCTCT TGGAAGAGAG CGCGTCTCGG 
CAGACGGATC TGGAGGCCAC GCTCTCTGCC AGGGACAAGG AGATCGCCTA CAGGAAGGCG 
GCGTTTCTTC AGCTGTCCTC TCAGAAGGAC GCGCTCTCGC AAGAAGCGTC CAGCCTTCGG 
CAGAGTGTCG CGAGCCAGCG GGAGACAAAC GAGCAGCTCA GCGCACAGAA GGAAGAGCTG 
GCCACGGCCA AGTCTCAGCT GGAAATGGAG CTACGGCACT GCCGGGCCGA GGGGGACAAG 
CTCCAGAGCG ACCTGACTGG CTGCAAGGAA GCCCGGGAAG CGACAGAAGT GGACCTGCGG 
AACGCCAGGA ATTCCCTCGC AAAGATCCAG CGGGAGAAAG AGACGACGGA CGCCAAGCTT 
CGGGACTCAG CGGCAGAGCT GGAGAAATTG CACGAGAAGC TGAAGAGGCT GGAAAGGGAG 
CGAAAAGCCG ATGCCGAGGA GTGGGGCATG CTGCGGGCAT CCCTGACCGA GAAGCTGCAA 
GCTGCGGAGG AGGCGGCGTT GCGGAGGGTA GAAGAGCAAC GGCGGCTGTT CAAGGAAGAG 
ATGGACAAAC ACCGGCAGGA GAAGGCTGGC CAATTGCGGC AGCTCGAGGA AAGCCACGCA 
GCAGAACTGA AGCGCCAGGA AGAGCTGCTG AGCGAGACAG AAACCAAGTG GCACAGCGAG 
GTGGCCCTGC ACCAGCAGAG GCTGGAAGAC GTGAGGGTGG AGCTCCAGGA AAAGCTAGCT 
TCTTCCGAGC AGCTCAGAGC CGAGATGCAG TCTATGGCGG AGGACGAGCG CAGGGAGCTG 
CTGAACTCTC TCGGCCGCTG CAAGGAGGAA GTGTCCGTGC TCCGGCGAAA CCTGGTGGCA 
TTCAGGGATG AAGCAGGCTC CAAGATGGCC GCCGAGCAGG CCAAGGTGGC GTCTGCTCTG 
AAGGACGTCG ACACGCTTCG CGTGGAACTG GAGCAGACTA GGACGGCGTT GCGTTTGAAA 
GAGGCACAGG AAAAGTCACG TGAGTCGCAG TTTCACAAGG AACTCAGCCG GGTGACCGAA 
GAAAGGGAGC GTGCCATTCA GCGGCTGAAC GAGGTCGATG CCAGTCATGC GGCTCTTCAG 
CGCGAGCTGT CTGAAACTCT TGGCAAGAAA TCCGAAGGGG AGCGTCGAGT CGAAGTTCTG 
ACTCACCAGT TGTCCGAGGC ACGTCAGAGT TACACGTTGG TCACGGCAGA GACCGAGCAG 
CTTCGCCGAG TCATTGGGGA GCTGCAGCAG GAGTTGCGCA GCGCCACTCA GGAGAGGGAC 
GCCTCGCTTC AGGAGGCTTC CGAAAAATTC CGCGGCGCGG AGAAAGCGCG GGCGACGTTG 
GCGGAGGAGC TTCGCGAGCT CCGGAACACG CACGGCGGCG TAGAGCGGGC TCGGCAGGTC 
TTGGAGCGCA AGGTGCGAGC CCTGGAGTCG GCTTTGCTGG AGCACGGCTC GGAGCTGGAC 
TCGCTCCGGA AGCTGTCCGA GCAGAGGGGA CATCAGGTGG AATCGGCACG TCAGGACGTT 
CTCTCCTGGA AGTGGAAGGT TGCCGTGCTC GAGGAAGAGC GGGACGCGTG GCAGCGCGAG 
CGTGCCTCCT TGGGCCGTCG TCTGGACGAG AGCGAGCGCC GGGCACAGGC GGAGCGCAAC 
GCTCGTGAAG GGGCGGTGCG CGAGGCCGGC CGGGTTGCCG AGAGACTGGC CCAGGACCGG 
GCCGACCTGG AGAAGCAGCT GCTGGCATCC GGGCAAGCAA CGCACGAGGC ACGCCTGGCA 
CTGTGCGCCG CGCAGGGCTC CACGGCTGCC CTCGAGAGCG AGCTCAAGGT CGTCTCCGAG 
TCTCGGAAGT CGATCGAGGG CACTCTGGAC TCGCTGCTGA GCGTGCTGCG CTGCTCCCTG 
GGGATCCAGA CCAAATACGA GCTGCGGTCC CCCTCCCCCC AGCGACCGGC GGCGGGCCGG 
CGCCACGGCG GCGATCCGTC CAACGAGAGC TTTCGGGGGG ACAGCTCGCC CTTTCGGTCG 
GAGGACTCCC TCGGCCGCTC CCTGCTGCGG CGGCCCACCC CCGACGTCGA GGCCGAGCTC 
GTGCTACAAG CCGTGGACGC CCTGTCGCAC CGCATCGCCA CGCTTGAAGC CGACAAGGAG 
AAGGTGGAGG CTGAGTGCGA GCGTTCCCAG CGGGTGGTTG CCGGACTGGA GGACGAGCTC 
ACGGCCACAA AGTCCGCGCT TGCGCGACTG ACCAAGGCAA ACGATGCTCT CGTTGCAGAC 
AATGCGGAGC TGCGCTCGCA AGTGTCCTCT CAGCTGGAGG CGGTCTCGGA GCTCAAACAC 
ACGTCCCAGA CCCTGGAGTG GGAGAAAGCG TCCCTCGTGG ACCAGCTGGC AGACGCAGCG 
CGCCGCTACC AGCTCAAGAC TGACCAGTGC GACAAACTTC AGGCCGCCCT GAGTCGCAGC 
CGCGACCACC CACCATCCCC GATTCGGCCC TCCGAGCCCC TCGAGTCTCG GCTGTCGAAG 
CTGGAGCTCG AGCGACAACT CTCGCAAGCC AAGGTCGAGA GGCTCAAGCT CAGGGTGACC 
GAGAGCCAGG CCGAAGTGAG GAGGCTGCAG GAAGCCAAGG AGGAAGCCAC TTCCCAGCTG 
ACTCGGCTCG AGAGGAGGGC GGAGAGCCTC GACCACGGCA ACAGCCGCCT GAGGGCAGAG 
ATTGCGGAGC ATGCGTCCAA GGTGAAGCTC CTGAACGCTC ACCTGGTGGA AGAGATTGCC 
AAGGGACGCG GCCTGGAGGA GGAGAAGCGA TCTCTGGAAA GCCAGCTCAG GGTGGCCAGG 
GTGCAGCTCG AGTCTGCCAG GTTCGACCGG AAAAGAGATG GGTCGGTGAT TGACGAGCTG 
AAGGGCCAGA AGGGCACTCT GGAGACCAGG CTGTCTGCGC TCGCCGTACA GCTGGCAGAA 
TGCGAGTCAG CCAAGCAGCG CCTCGACAAG ACGCCCATCA GGACTCCCCA CAGGGAAGAG 
ACCAGGACTT GGCAGCAGAT GGTGGAGACG TTGCGCGAGG AGAAGCGGCA GCTCCAGGGG 
GAGGTGACGC GCCTCCGGCG GCAGCTCACG GGCACCATTG ACAGTATCCT GCCGCCGGCG 
ACTCCCGACG GGAGCCGAGC TTCGAGGCGG ACACGCCGCA GTCTGGCAGA GCGTGACGTG 
CCAGACGGCG CAAGCCTCCA TTCTCCCGAG GTGTCTCGCC TGAAGACGCA GAAGAGCCTC 
CTGGAGGCCA AAGTCCGCAC TTTGGAGGAG CGCCTCTCTC AGCAAGACCA GGTGCACTCG 
GAGGAGCTGA CGCGGGAACA CCGGGAGCTG GTCGCCGAGA TCGCCAGACT GCGGGAGGAG 
CTGGCAACGC ACGAGCGGCG CCGGACGTCG ACCCCCGTGC ACAACGAGCC TCCCGAGTTT 
GCCATCAATC TAAGCCACAG GCAAGACATC CAAGGGCTGC TCGACCGTTC CCTCCACACC 
GTGTCCGAAG ACCCGGAGCG GCTGCGCGAC CAAGCCATGT TGCTCAGCGC GTTCATCGAC 
GGAGCACTGG AACCGGGGGA CAGCCTTCGG ACCCCTTCTA AGCTTGCGCC AAACAAGCGG 
ACCACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8029650 ( XM_029995333.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029851193.1 Ixodes scapularis rootletin (LOC8029650), transcript variant X3, mRNA.

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CloneID	OIa36136
Clone ID Related Accession (Same CDS sequence)	XM_029995333.1
Accession Version	XM_029995333.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	rootletin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021922528.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATATC TTGGCGACGG GCAAGTTCAG GCGGTTCAGG CACTCGGTGG TTCATTCCAA 
CATTCTGGTG GAGCGGTATT TGAAAGCCCG CTTCGCATTG CAGTGATGGA GCAGCCACCG 
GAGAACGTAA TTGCGAGGCT ACCGTCTTCG TCGACCATCA ATGACGAGGA CTATGCAACG 
GCAGAGGAGG AACCACCAGG CAGAACCTCG AGGCTCGAGG ACGACGACCA GCAGGGCGTG 
CTCTCGGACG CCTGCTCGAC CGACAGCGAC CACGAGCTCG AGTTGGTGGG TCTGGATCGC 
GAACACGAGC TCAAGTCGAA GCTGGAGAAG TCGAGGACCA TGTTGCGGGC CTACGTGCAG 
CACACGACCA ATCAGGACAG GCTGCTTGGG CACCTGGGAG CACAGGTGGA GGAGTACCGG 
AACCGCTGCT CGAGATTGGA AGAGGTCCTC CGCCAGTGCC CGTGTGCCGA TCGAACTGAG 
AACATTCAAG ACGATCGCTC GTACGAAGAA GATCCTCCTG ACCTTCCGTC GGCCTTATCA 
GCGCTGGAAC TAGAGAGGGC AAGATGTGCG GAGCTGAAGC AGCTGAACGT GGTCCTCCGC 
GAGCACCTGG AGATAACGAC GGAGACGAAT GCGACCCTCA CCATGCACAT GGAGAGGATG 
ACGGAGGACT GGCAGAACGC GACCCGGGCT GACGCGGTCA ATCGCGGCAG CAGCAGCAGC 
GCCACCCACG ACGAGTTTCG CGCAGACTCC GAGCAGGGCA GCGTTCCCAG GCTGCGTCGA 
GACTTGCAGG AGCTGAAGGG GGACCTGGGA GACATGAGGC AGGCCTTTGC CACCAGCCTG 
GCCTCCATCC AGACGATGCT GCACGACGCC GCCGAGGCGG CCGGGCGTGT CCAACTCTCG 
CTTGTGGACG ACGCGGCGGA CAGCGACCAG CCAGACCTCG GGCTCACCAG CAGCCAGGAA 
AACGTGGACC TGCGCGTGCG GGAAGCCATG CTACAGTCGG AGGTGCGCAG CCACGAGGCC 
ACGTCGAAGA ATCTCGCCTT GGAGCTGGAG TCCGTCCAAA AGGAAAAGAA AGAGTGCATG 
GGTGTAATTG CGTCGCTGCG CAAGCATGTC GCCAACCTCG AGGACCAGCA ACAGGTGTCC 
GAACGCGAGC TCAGCGTTCT GAGGAAAGCG GTGCGTGACA TCGAAGAACT CAACGACTCC 
CTGGAACGGG AGATGCAAGA GATGTGCAAC GCCAGCATGC AGCTTAAACA GTCCAACTCG 
GAGCTGGCAA AGGCAAAGAG TGACATGGAA CTTGCGCACG AAGAGTTGGC CGCGGAACGG 
GACTCCCTGG CACGACGGGA AGCGGATGTA ACGCGAGCGA TGGCCCGTCT GGAGTCGCTC 
TTGGAAGAGA GCGCGTCTCG GCAGACGGAT CTGGAGGCCA CGCTCTCTGC CAGGGACAAG 
GAGATCGCCT ACAGGAAGGC GGCGTTTCTT CAGCTGTCCT CTCAGAAGGA CGCGCTCTCG 
CAAGAAGCGT CCAGCCTTCG GCAGAGTGTC GCGAGCCAGC GGGAGACAAA CGAGCAGCTC 
AGCGCACAGA AGGAAGAGCT GGCCACGGCC AAGTCTCAGC TGGAAATGGA GCTACGGCAC 
TGCCGGGCCG AGGGGGACAA GCTCCAGAGC GACCTGACTG GCTGCAAGGA AGCCCGGGAA 
GCGACAGAAG TGGACCTGCG GAACGCCAGG AATTCCCTCG CAAAGATCCA GCGGGAGAAA 
GAGACGACGG ACGCCAAGCT TCGGGACTCA GCGGCAGAGC TGGAGAAATT GCACGAGAAG 
CTGAAGAGGC TGGAAAGGGA GCGAAAAGCC GATGCCGAGG AGTGGGGCAT GCTGCGGGCA 
TCCCTGACCG AGAAGCTGCA AGCTGCGGAG GAGGCGGCGT TGCGGAGGGT AGAAGAGCAA 
CGGCGGCTGT TCAAGGAAGA GATGGACAAA CACCGGCAGG AGAAGGCTGG CCAATTGCGG 
CAGCTCGAGG AAAGCCACGC AGCAGAACTG AAGCGCCAGG AAGAGCTGCT GAGCGAGACA 
GAAACCAAGT GGCACAGCGA GGTGGCCCTG CACCAGCAGA GGCTGGAAGA CGTGAGGGTG 
GAGCTCCAGG AAAAGCTAGC TTCTTCCGAG CAGCTCAGAG CCGAGATGCA GTCTATGGCG 
GAGGACGAGC GCAGGGAGCT GCTGAACTCT CTCGGCCGCT GCAAGGAGGA AGTGTCCGTG 
CTCCGGCGAA ACCTGGTGGC ATTCAGGGAT GAAGCAGGCT CCAAGATGGC CGCCGAGCAG 
GCCAAGGTGG CGTCTGCTCT GAAGGACGTC GACACGCTTC GCGTGGAACT GGAGCAGACT 
AGGACGGCGT TGCGTTTGAA AGAGGCACAG GAAAAGTCAC GTGAGTCGCA GTTTCACAAG 
GAACTCAGCC GGGTGACCGA AGAAAGGGAG CGTGCCATTC AGCGGCTGAA CGAGGTCGAT 
GCCAGTCATG CGGCTCTTCA GCGCGAGCTG TCTGAAACTC TTGGCAAGAA ATCCGAAGGG 
GAGCGTCGAG TCGAAGTTCT GACTCACCAG TTGTCCGAGG CACGTCAGAG TTACACGTTG 
GTCACGGCAG AGACCGAGCA GCTTCGCCGA GTCATTGGGG AGCTGCAGCA GGAGTTGCGC 
AGCGCCACTC AGGAGAGGGA CGCCTCGCTT CAGGAGGCTT CCGAAAAATT CCGCGGCGCG 
GAGAAAGCGC GGGCGACGTT GGCGGAGGAG CTTCGCGAGC TCCGGAACAC GCACGGCGGC 
GTAGAGCGGG CTCGGCAGGT CTTGGAGCGC AAGGTGCGAG CCCTGGAGTC GGCTTTGCTG 
GAGCACGGCT CGGAGCTGGA CTCGCTCCGG AAGCTGTCCG AGCAGAGGGG ACATCAGGTG 
GAATCGGCAC GTCAGGACGT TCTCTCCTGG AAGTGGAAGG TTGCCGTGCT CGAGGAAGAG 
CGGGACGCGT GGCAGCGCGA GCGTGCCTCC TTGGGCCGTC GTCTGGACGA GAGCGAGCGC 
CGGGCACAGG CGGAGCGCAA CGCTCGTGAA GGGGCGGTGC GCGAGGCCGG CCGGGTTGCC 
GAGAGACTGG CCCAGGACCG GGCCGACCTG GAGAAGCAGC TGCTGGCATC CGGGCAAGCA 
ACGCACGAGG CACGCCTGGC ACTGTGCGCC GCGCAGGGCT CCACGGCTGC CCTCGAGAGC 
GAGCTCAAGG TCGTCTCCGA GTCTCGGAAG TCGATCGAGG GCACTCTGGA CTCGCTGCTG 
AGCGTGCTGC GCTGCTCCCT GGGGATCCAG ACCAAATACG AGCTGCGGTC CCCCTCCCCC 
CAGCGACCGG CGGCGGGCCG GCGCCACGGC GGCGATCCGT CCAACGAGAG CTTTCGGGGG 
GACAGCTCGC CCTTTCGGTC GGAGGACTCC CTCGGCCGCT CCCTGCTGCG GCGGCCCACC 
CCCGACGTCG AGGCCGAGCT CGTGCTACAA GCCGTGGACG CCCTGTCGCA CCGCATCGCC 
ACGCTTGAAG CCGACAAGGA GAAGGTGGAG GCTGAGTGCG AGCGTTCCCA GCGGGTGGTT 
GCCGGACTGG AGGACGAGCT CACGGCCACA AAGTCCGCGC TTGCGCGACT GACCAAGGCA 
AACGATGCTC TCGTTGCAGA CAATGCGGAG CTGCGCTCGC AAGTGTCCTC TCAGCTGGAG 
GCGGTCTCGG AGCTCAAACA CACGTCCCAG ACCCTGGAGT GGGAGAAAGC GTCCCTCGTG 
GACCAGCTGG CAGACGCAGC GCGCCGCTAC CAGCTCAAGA CTGACCAGTG CGACAAACTT 
CAGGCCGCCC TGAGTCGCAG CCGCGACCAC CCACCATCCC CGATTCGGCC CTCCGAGCCC 
CTCGAGTCTC GGCTGTCGAA GCTGGAGCTC GAGCGACAAC TCTCGCAAGC CAAGGTCGAG 
AGGCTCAAGC TCAGGGTGAC CGAGAGCCAG GCCGAAGTGA GGAGGCTGCA GGAAGCCAAG 
GAGGAAGCCA CTTCCCAGCT GACTCGGCTC GAGAGGAGGG CGGAGAGCCT CGACCACGGC 
AACAGCCGCC TGAGGGCAGA GATTGCGGAG CATGCGTCCA AGGTGAAGCT CCTGAACGCT 
CACCTGGTGG AAGAGATTGC CAAGGGACGC GGCCTGGAGG AGGAGAAGCG ATCTCTGGAA 
AGCCAGCTCA GGGTGGCCAG GGTGCAGCTC GAGTCTGCCA GGTTCGACCG GAAAAGAGAT 
GGGTCGGTGA TTGACGAGCT GAAGGGCCAG AAGGGCACTC TGGAGACCAG GCTGTCTGCG 
CTCGCCGTAC AGCTGGCAGA ATGCGAGTCA GCCAAGCAGC GCCTCGACAA GACGCCCATC 
AGGACTCCCC ACAGGGAAGA GACCAGGACT TGGCAGCAGA TGGTGGAGAC GTTGCGCGAG 
GAGAAGCGGC AGCTCCAGGG GGAGGTGACG CGCCTCCGGC GGCAGCTCAC GGGCACCATT 
GACAGTATCC TGCCGCCGGC GACTCCCGAC GGGAGCCGAG CTTCGAGGCG GACACGCCGC 
AGTCTGGCAG AGCGTGACGT GCCAGACGGC GCAAGCCTCC ATTCTCCCGA GGTGTCTCGC 
CTGAAGACGC AGAAGAGCCT CCTGGAGGCC AAAGTCCGCA CTTTGGAGGA GCGCCTCTCT 
CAGCAAGACC AGGTGCACTC GGAGGAGCTG ACGCGGGAAC ACCGGGAGCT GGTCGCCGAG 
ATCGCCAGAC TGCGGGAGGA GCTGGCAACG CACGAGCGGC GCCGGACGTC GACCCCCGTG 
CACAACGAGC CTCCCGAGTT TGCCATCAAT CTAAGCCACA GGCAAGACAT CCAAGGGCTG 
CTCGACCGTT CCCTCCACAC CGTGTCCGAA GACCCGGAGC GGCTGCGCGA CCAAGCCATG 
TTGCTCAGCG CGTTCATCGA CGGAGCACTG GAACCGGGGG ACAGCCTTCG GACCCCTTCT 
AAGCTTGCGC CAAACAAGCG GACCACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029851193.1
CDS	110..5059
Translation	MTYLGDGQVQAVQALGGSFQHSGGAVFESPLRIAVMEQPPENVIARLPSSSTINDEDYATAEEEPPGRTSRLEDDDQQGVLSDACSTDSDHELELVGLDREHELKSKLEKSRTMLRAYVQHTTNQDRLLGHLGAQVEEYRNRCSRLEEVLRQCPCADRTENIQDDRSYEEDPPDLPSALSALELERARCAELKQLNVVLREHLEITTETNATLTMHMERMTEDWQNATRADAVNRGSSSSATHDEFRADSEQGSVPRLRRDLQELKGDLGDMRQAFATSLASIQTMLHDAAEAAGRVQLSLVDDAADSDQPDLGLTSSQENVDLRVREAMLQSEVRSHEATSKNLALELESVQKEKKECMGVIASLRKHVANLEDQQQVSERELSVLRKAVRDIEELNDSLEREMQEMCNASMQLKQSNSELAKAKSDMELAHEELAAERDSLARREADVTRAMARLESLLEESASRQTDLEATLSARDKEIAYRKAAFLQLSSQKDALSQEASSLRQSVASQRETNEQLSAQKEELATAKSQLEMELRHCRAEGDKLQSDLTGCKEAREATEVDLRNARNSLAKIQREKETTDAKLRDSAAELEKLHEKLKRLERERKADAEEWGMLRASLTEKLQAAEEAALRRVEEQRRLFKEEMDKHRQEKAGQLRQLEESHAAELKRQEELLSETETKWHSEVALHQQRLEDVRVELQEKLASSEQLRAEMQSMAEDERRELLNSLGRCKEEVSVLRRNLVAFRDEAGSKMAAEQAKVASALKDVDTLRVELEQTRTALRLKEAQEKSRESQFHKELSRVTEERERAIQRLNEVDASHAALQRELSETLGKKSEGERRVEVLTHQLSEARQSYTLVTAETEQLRRVIGELQQELRSATQERDASLQEASEKFRGAEKARATLAEELRELRNTHGGVERARQVLERKVRALESALLEHGSELDSLRKLSEQRGHQVESARQDVLSWKWKVAVLEEERDAWQRERASLGRRLDESERRAQAERNAREGAVREAGRVAERLAQDRADLEKQLLASGQATHEARLALCAAQGSTAALESELKVVSESRKSIEGTLDSLLSVLRCSLGIQTKYELRSPSPQRPAAGRRHGGDPSNESFRGDSSPFRSEDSLGRSLLRRPTPDVEAELVLQAVDALSHRIATLEADKEKVEAECERSQRVVAGLEDELTATKSALARLTKANDALVADNAELRSQVSSQLEAVSELKHTSQTLEWEKASLVDQLADAARRYQLKTDQCDKLQAALSRSRDHPPSPIRPSEPLESRLSKLELERQLSQAKVERLKLRVTESQAEVRRLQEAKEEATSQLTRLERRAESLDHGNSRLRAEIAEHASKVKLLNAHLVEEIAKGRGLEEEKRSLESQLRVARVQLESARFDRKRDGSVIDELKGQKGTLETRLSALAVQLAECESAKQRLDKTPIRTPHREETRTWQQMVETLREEKRQLQGEVTRLRRQLTGTIDSILPPATPDGSRASRRTRRSLAERDVPDGASLHSPEVSRLKTQKSLLEAKVRTLEERLSQQDQVHSEELTREHRELVAEIARLREELATHERRRTSTPVHNEPPEFAINLSHRQDIQGLLDRSLHTVSEDPERLRDQAMLLSAFIDGALEPGDSLRTPSKLAPNKRTT

Target ORF information:

RefSeq Version	XM_029995333.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis rootletin (LOC8029650), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029995333.1

ORF Insert Sequence:

ATGACATATC TTGGCGACGG GCAAGTTCAG GCGGTTCAGG CACTCGGTGG TTCATTCCAA 
CATTCTGGTG GAGCGGTATT TGAAAGCCCG CTTCGCATTG CAGTGATGGA GCAGCCACCG 
GAGAACGTAA TTGCGAGGCT ACCGTCTTCG TCGACCATCA ATGACGAGGA CTATGCAACG 
GCAGAGGAGG AACCACCAGG CAGAACCTCG AGGCTCGAGG ACGACGACCA GCAGGGCGTG 
CTCTCGGACG CCTGCTCGAC CGACAGCGAC CACGAGCTCG AGTTGGTGGG TCTGGATCGC 
GAACACGAGC TCAAGTCGAA GCTGGAGAAG TCGAGGACCA TGTTGCGGGC CTACGTGCAG 
CACACGACCA ATCAGGACAG GCTGCTTGGG CACCTGGGAG CACAGGTGGA GGAGTACCGG 
AACCGCTGCT CGAGATTGGA AGAGGTCCTC CGCCAGTGCC CGTGTGCCGA TCGAACTGAG 
AACATTCAAG ACGATCGCTC GTACGAAGAA GATCCTCCTG ACCTTCCGTC GGCCTTATCA 
GCGCTGGAAC TAGAGAGGGC AAGATGTGCG GAGCTGAAGC AGCTGAACGT GGTCCTCCGC 
GAGCACCTGG AGATAACGAC GGAGACGAAT GCGACCCTCA CCATGCACAT GGAGAGGATG 
ACGGAGGACT GGCAGAACGC GACCCGGGCT GACGCGGTCA ATCGCGGCAG CAGCAGCAGC 
GCCACCCACG ACGAGTTTCG CGCAGACTCC GAGCAGGGCA GCGTTCCCAG GCTGCGTCGA 
GACTTGCAGG AGCTGAAGGG GGACCTGGGA GACATGAGGC AGGCCTTTGC CACCAGCCTG 
GCCTCCATCC AGACGATGCT GCACGACGCC GCCGAGGCGG CCGGGCGTGT CCAACTCTCG 
CTTGTGGACG ACGCGGCGGA CAGCGACCAG CCAGACCTCG GGCTCACCAG CAGCCAGGAA 
AACGTGGACC TGCGCGTGCG GGAAGCCATG CTACAGTCGG AGGTGCGCAG CCACGAGGCC 
ACGTCGAAGA ATCTCGCCTT GGAGCTGGAG TCCGTCCAAA AGGAAAAGAA AGAGTGCATG 
GGTGTAATTG CGTCGCTGCG CAAGCATGTC GCCAACCTCG AGGACCAGCA ACAGGTGTCC 
GAACGCGAGC TCAGCGTTCT GAGGAAAGCG GTGCGTGACA TCGAAGAACT CAACGACTCC 
CTGGAACGGG AGATGCAAGA GATGTGCAAC GCCAGCATGC AGCTTAAACA GTCCAACTCG 
GAGCTGGCAA AGGCAAAGAG TGACATGGAA CTTGCGCACG AAGAGTTGGC CGCGGAACGG 
GACTCCCTGG CACGACGGGA AGCGGATGTA ACGCGAGCGA TGGCCCGTCT GGAGTCGCTC 
TTGGAAGAGA GCGCGTCTCG GCAGACGGAT CTGGAGGCCA CGCTCTCTGC CAGGGACAAG 
GAGATCGCCT ACAGGAAGGC GGCGTTTCTT CAGCTGTCCT CTCAGAAGGA CGCGCTCTCG 
CAAGAAGCGT CCAGCCTTCG GCAGAGTGTC GCGAGCCAGC GGGAGACAAA CGAGCAGCTC 
AGCGCACAGA AGGAAGAGCT GGCCACGGCC AAGTCTCAGC TGGAAATGGA GCTACGGCAC 
TGCCGGGCCG AGGGGGACAA GCTCCAGAGC GACCTGACTG GCTGCAAGGA AGCCCGGGAA 
GCGACAGAAG TGGACCTGCG GAACGCCAGG AATTCCCTCG CAAAGATCCA GCGGGAGAAA 
GAGACGACGG ACGCCAAGCT TCGGGACTCA GCGGCAGAGC TGGAGAAATT GCACGAGAAG 
CTGAAGAGGC TGGAAAGGGA GCGAAAAGCC GATGCCGAGG AGTGGGGCAT GCTGCGGGCA 
TCCCTGACCG AGAAGCTGCA AGCTGCGGAG GAGGCGGCGT TGCGGAGGGT AGAAGAGCAA 
CGGCGGCTGT TCAAGGAAGA GATGGACAAA CACCGGCAGG AGAAGGCTGG CCAATTGCGG 
CAGCTCGAGG AAAGCCACGC AGCAGAACTG AAGCGCCAGG AAGAGCTGCT GAGCGAGACA 
GAAACCAAGT GGCACAGCGA GGTGGCCCTG CACCAGCAGA GGCTGGAAGA CGTGAGGGTG 
GAGCTCCAGG AAAAGCTAGC TTCTTCCGAG CAGCTCAGAG CCGAGATGCA GTCTATGGCG 
GAGGACGAGC GCAGGGAGCT GCTGAACTCT CTCGGCCGCT GCAAGGAGGA AGTGTCCGTG 
CTCCGGCGAA ACCTGGTGGC ATTCAGGGAT GAAGCAGGCT CCAAGATGGC CGCCGAGCAG 
GCCAAGGTGG CGTCTGCTCT GAAGGACGTC GACACGCTTC GCGTGGAACT GGAGCAGACT 
AGGACGGCGT TGCGTTTGAA AGAGGCACAG GAAAAGTCAC GTGAGTCGCA GTTTCACAAG 
GAACTCAGCC GGGTGACCGA AGAAAGGGAG CGTGCCATTC AGCGGCTGAA CGAGGTCGAT 
GCCAGTCATG CGGCTCTTCA GCGCGAGCTG TCTGAAACTC TTGGCAAGAA ATCCGAAGGG 
GAGCGTCGAG TCGAAGTTCT GACTCACCAG TTGTCCGAGG CACGTCAGAG TTACACGTTG 
GTCACGGCAG AGACCGAGCA GCTTCGCCGA GTCATTGGGG AGCTGCAGCA GGAGTTGCGC 
AGCGCCACTC AGGAGAGGGA CGCCTCGCTT CAGGAGGCTT CCGAAAAATT CCGCGGCGCG 
GAGAAAGCGC GGGCGACGTT GGCGGAGGAG CTTCGCGAGC TCCGGAACAC GCACGGCGGC 
GTAGAGCGGG CTCGGCAGGT CTTGGAGCGC AAGGTGCGAG CCCTGGAGTC GGCTTTGCTG 
GAGCACGGCT CGGAGCTGGA CTCGCTCCGG AAGCTGTCCG AGCAGAGGGG ACATCAGGTG 
GAATCGGCAC GTCAGGACGT TCTCTCCTGG AAGTGGAAGG TTGCCGTGCT CGAGGAAGAG 
CGGGACGCGT GGCAGCGCGA GCGTGCCTCC TTGGGCCGTC GTCTGGACGA GAGCGAGCGC 
CGGGCACAGG CGGAGCGCAA CGCTCGTGAA GGGGCGGTGC GCGAGGCCGG CCGGGTTGCC 
GAGAGACTGG CCCAGGACCG GGCCGACCTG GAGAAGCAGC TGCTGGCATC CGGGCAAGCA 
ACGCACGAGG CACGCCTGGC ACTGTGCGCC GCGCAGGGCT CCACGGCTGC CCTCGAGAGC 
GAGCTCAAGG TCGTCTCCGA GTCTCGGAAG TCGATCGAGG GCACTCTGGA CTCGCTGCTG 
AGCGTGCTGC GCTGCTCCCT GGGGATCCAG ACCAAATACG AGCTGCGGTC CCCCTCCCCC 
CAGCGACCGG CGGCGGGCCG GCGCCACGGC GGCGATCCGT CCAACGAGAG CTTTCGGGGG 
GACAGCTCGC CCTTTCGGTC GGAGGACTCC CTCGGCCGCT CCCTGCTGCG GCGGCCCACC 
CCCGACGTCG AGGCCGAGCT CGTGCTACAA GCCGTGGACG CCCTGTCGCA CCGCATCGCC 
ACGCTTGAAG CCGACAAGGA GAAGGTGGAG GCTGAGTGCG AGCGTTCCCA GCGGGTGGTT 
GCCGGACTGG AGGACGAGCT CACGGCCACA AAGTCCGCGC TTGCGCGACT GACCAAGGCA 
AACGATGCTC TCGTTGCAGA CAATGCGGAG CTGCGCTCGC AAGTGTCCTC TCAGCTGGAG 
GCGGTCTCGG AGCTCAAACA CACGTCCCAG ACCCTGGAGT GGGAGAAAGC GTCCCTCGTG 
GACCAGCTGG CAGACGCAGC GCGCCGCTAC CAGCTCAAGA CTGACCAGTG CGACAAACTT 
CAGGCCGCCC TGAGTCGCAG CCGCGACCAC CCACCATCCC CGATTCGGCC CTCCGAGCCC 
CTCGAGTCTC GGCTGTCGAA GCTGGAGCTC GAGCGACAAC TCTCGCAAGC CAAGGTCGAG 
AGGCTCAAGC TCAGGGTGAC CGAGAGCCAG GCCGAAGTGA GGAGGCTGCA GGAAGCCAAG 
GAGGAAGCCA CTTCCCAGCT GACTCGGCTC GAGAGGAGGG CGGAGAGCCT CGACCACGGC 
AACAGCCGCC TGAGGGCAGA GATTGCGGAG CATGCGTCCA AGGTGAAGCT CCTGAACGCT 
CACCTGGTGG AAGAGATTGC CAAGGGACGC GGCCTGGAGG AGGAGAAGCG ATCTCTGGAA 
AGCCAGCTCA GGGTGGCCAG GGTGCAGCTC GAGTCTGCCA GGTTCGACCG GAAAAGAGAT 
GGGTCGGTGA TTGACGAGCT GAAGGGCCAG AAGGGCACTC TGGAGACCAG GCTGTCTGCG 
CTCGCCGTAC AGCTGGCAGA ATGCGAGTCA GCCAAGCAGC GCCTCGACAA GACGCCCATC 
AGGACTCCCC ACAGGGAAGA GACCAGGACT TGGCAGCAGA TGGTGGAGAC GTTGCGCGAG 
GAGAAGCGGC AGCTCCAGGG GGAGGTGACG CGCCTCCGGC GGCAGCTCAC GGGCACCATT 
GACAGTATCC TGCCGCCGGC GACTCCCGAC GGGAGCCGAG CTTCGAGGCG GACACGCCGC 
AGTCTGGCAG AGCGTGACGT GCCAGACGGC GCAAGCCTCC ATTCTCCCGA GGTGTCTCGC 
CTGAAGACGC AGAAGAGCCT CCTGGAGGCC AAAGTCCGCA CTTTGGAGGA GCGCCTCTCT 
CAGCAAGACC AGGTGCACTC GGAGGAGCTG ACGCGGGAAC ACCGGGAGCT GGTCGCCGAG 
ATCGCCAGAC TGCGGGAGGA GCTGGCAACG CACGAGCGGC GCCGGACGTC GACCCCCGTG 
CACAACGAGC CTCCCGAGTT TGCCATCAAT CTAAGCCACA GGCAAGACAT CCAAGGGCTG 
CTCGACCGTT CCCTCCACAC CGTGTCCGAA GACCCGGAGC GGCTGCGCGA CCAAGCCATG 
TTGCTCAGCG CGTTCATCGA CGGAGCACTG GAACCGGGGG ACAGCCTTCG GACCCCTTCT 
AAGCTTGCGC CAAACAAGCG GACCACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8029650 ( XM_029995334.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029851194.1 Ixodes scapularis rootletin (LOC8029650), transcript variant X4, mRNA.

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CloneID	OIa36137
Clone ID Related Accession (Same CDS sequence)	XM_029995334.1
Accession Version	XM_029995334.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	rootletin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021922528.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATATC TTGGCGACGG GCAAGTTCAG GCGGTTCAGG CACTCGGTGG TTCATTCCAA 
CATTCTGGTG GAGCGGTATT TGAAAGCCCG CTTCGCATTG CAGTGATGGA GCAGCCACCG 
GAGAACGTAA TTGCGAGGCT ACCGTCTTCG TCGACCATCA ATGACGAGGA CTATGCAACG 
GCAGAGGAGG AACCACCAGG CAGAACCTCG AGGCTCGAGG ACGACGACCA GCAGGGCGTG 
CTCTCGGACG CCTGCTCGAC CGACAGCGAC CACGAGCTCG AGTTGGTGGG TCTGGATCGC 
GAACACGAGC TCAAGTCGAA GCTGGAGAAG TCGAGGACCA TGTTGCGGGC CTACGTGCAG 
CACACGACCA ATCAGGACAG GCTGCTTGGG CACCTGGGAG CACAGGTGGA GGAGTACCGG 
AACCGCTGCT CGAGATTGGA AGAGGTCCTC CGCCAGTGCC CGTGTGCCGA TCGAACTGAG 
AACATTCAAG ACGATCGCTC GTACGAAGAA GATCCTCCTG ACCTTCCGTC GGCCTTATCA 
GCGCTGGAAC TAGAGAGGGC AAGATGTGCG GAGCTGAAGC AGCTGAACGT GGTCCTCCGC 
GAGCACCTGG AGATAACGAC GGAGACGAAT GCGACCCTCA CCATGCACAT GGAGAGGATG 
ACGGAGGACT GGCAGAACGC GACCCGGGCT GACGCGGTCA ATCGCGGCAG CAGCAGCAGC 
GCCACCCACG ACGAGTTTCG CGCAGACTCC GAGCAGGGCA GCGTTCCCAG GCTGCGTCGA 
GACTTGCAGG AGCTGAAGGG GGACCTGGGA GACATGAGGC AGGCCTTTGC CACCAGCCTG 
GCCTCCATCC AGACGATGCT GCACGACGCC GCCGAGGCGG CCGGGCGTGT CCAACTCTCG 
CTTGTGGACG ACGCGGCGGA CAGCGACCAG CCAGACCTCG GGCTCACCAG CCAGGAAAAC 
GTGGACCTGC GCGTGCGGGA AGCCATGCTA CAGTCGGAGG TGCGCAGCCA CGAGGCCACG 
TCGAAGAATC TCGCCTTGGA GCTGGAGTCC GTCCAAAAGG AAAAGAAAGA GTGCATGGGT 
GTAATTGCGT CGCTGCGCAA GCATGTCGCC AACCTCGAGG ACCAGCAACA GGTGTCCGAA 
CGCGAGCTCA GCGTTCTGAG GAAAGCGGTG CGTGACATCG AAGAACTCAA CGACTCCCTG 
GAACGGGAGA TGCAAGAGAT GTGCAACGCC AGCATGCAGC TTAAACAGTC CAACTCGGAG 
CTGGCAAAGG CAAAGAGTGA CATGGAACTT GCGCACGAAG AGTTGGCCGC GGAACGGGAC 
TCCCTGGCAC GACGGGAAGC GGATGTAACG CGAGCGATGG CCCGTCTGGA GTCGCTCTTG 
GAAGAGAGCG CGTCTCGGCA GACGGATCTG GAGGCCACGC TCTCTGCCAG GGACAAGGAG 
ATCGCCTACA GGAAGGCGGC GTTTCTTCAG CTGTCCTCTC AGAAGGACGC GCTCTCGCAA 
GAAGCGTCCA GCCTTCGGCA GAGTGTCGCG AGCCAGCGGG AGACAAACGA GCAGCTCAGC 
GCACAGAAGG AAGAGCTGGC CACGGCCAAG TCTCAGCTGG AAATGGAGCT ACGGCACTGC 
CGGGCCGAGG GGGACAAGCT CCAGAGCGAC CTGACTGGCT GCAAGGAAGC CCGGGAAGCG 
ACAGAAGTGG ACCTGCGGAA CGCCAGGAAT TCCCTCGCAA AGATCCAGCG GGAGAAAGAG 
ACGACGGACG CCAAGCTTCG GGACTCAGCG GCAGAGCTGG AGAAATTGCA CGAGAAGCTG 
AAGAGGCTGG AAAGGGAGCG AAAAGCCGAT GCCGAGGAGT GGGGCATGCT GCGGGCATCC 
CTGACCGAGA AGCTGCAAGC TGCGGAGGAG GCGGCGTTGC GGAGGGTAGA AGAGCAACGG 
CGGCTGTTCA AGGAAGAGAT GGACAAACAC CGGCAGGAGA AGGCTGGCCA ATTGCGGCAG 
CTCGAGGAAA GCCACGCAGC AGAACTGAAG CGCCAGGAAG AGCTGCTGAG CGAGACAGAA 
ACCAAGTGGC ACAGCGAGGT GGCCCTGCAC CAGCAGAGGC TGGAAGACGT GAGGGTGGAG 
CTCCAGGAAA AGCTAGCTTC TTCCGAGCAG CTCAGAGCCG AGATGCAGTC TATGGCGGAG 
GACGAGCGCA GGGAGCTGCT GAACTCTCTC GGCCGCTGCA AGGAGGAAGT GTCCGTGCTC 
CGGCGAAACC TGGTGGCATT CAGGGATGAA GCAGGCTCCA AGATGGCCGC CGAGCAGGCC 
AAGGTGGCGT CTGCTCTGAA GGACGTCGAC ACGCTTCGCG TGGAACTGGA GCAGACTAGG 
ACGGCGTTGC GTTTGAAAGA GGCACAGGAA AAGTCACGTG AGTCGCAGTT TCACAAGGAA 
CTCAGCCGGG TGACCGAAGA AAGGGAGCGT GCCATTCAGC GGCTGAACGA GGTCGATGCC 
AGTCATGCGG CTCTTCAGCG CGAGCTGTCT GAAACTCTTG GCAAGAAATC CGAAGGGGAG 
CGTCGAGTCG AAGTTCTGAC TCACCAGTTG TCCGAGGCAC GTCAGAGTTA CACGTTGGTC 
ACGGCAGAGA CCGAGCAGCT TCGCCGAGTC ATTGGGGAGC TGCAGCAGGA GTTGCGCAGC 
GCCACTCAGG AGAGGGACGC CTCGCTTCAG GAGGCTTCCG AAAAATTCCG CGGCGCGGAG 
AAAGCGCGGG CGACGTTGGC GGAGGAGCTT CGCGAGCTCC GGAACACGCA CGGCGGCGTA 
GAGCGGGCTC GGCAGGTCTT GGAGCGCAAG GTGCGAGCCC TGGAGTCGGC TTTGCTGGAG 
CACGGCTCGG AGCTGGACTC GCTCCGGAAG CTGTCCGAGC AGAGGGGACA TCAGGTGGAA 
TCGGCACGTC AGGACGTTCT CTCCTGGAAG TGGAAGGTTG CCGTGCTCGA GGAAGAGCGG 
GACGCGTGGC AGCGCGAGCG TGCCTCCTTG GGCCGTCGTC TGGACGAGAG CGAGCGCCGG 
GCACAGGCGG AGCGCAACGC TCGTGAAGGG GCGGTGCGCG AGGCCGGCCG GGTTGCCGAG 
AGACTGGCCC AGGACCGGGC CGACCTGGAG AAGCAGCTGC TGGCATCCGG GCAAGCAACG 
CACGAGGCAC GCCTGGCACT GTGCGCCGCG CAGGGCTCCA CGGCTGCCCT CGAGAGCGAG 
CTCAAGGTCG TCTCCGAGTC TCGGAAGTCG ATCGAGGGCA CTCTGGACTC GCTGCTGAGC 
GTGCTGCGCT GCTCCCTGGG GATCCAGACC AAATACGAGC TGCGGTCCCC CTCCCCCCAG 
CGACCGGCGG CGGGCCGGCG CCACGGCGGC GATCCGTCCA ACGAGAGCTT TCGGGGGGAC 
AGCTCGCCCT TTCGGTCGGA GGACTCCCTC GGCCGCTCCC TGCTGCGGCG GCCCACCCCC 
GACGTCGAGG CCGAGCTCGT GCTACAAGCC GTGGACGCCC TGTCGCACCG CATCGCCACG 
CTTGAAGCCG ACAAGGAGAA GGTGGAGGCT GAGTGCGAGC GTTCCCAGCG GGTGGTTGCC 
GGACTGGAGG ACGAGCTCAC GGCCACAAAG TCCGCGCTTG CGCGACTGAC CAAGGCAAAC 
GATGCTCTCG TTGCAGACAA TGCGGAGCTG CGCTCGCAAG TGTCCTCTCA GCTGGAGGCG 
GTCTCGGAGC TCAAACACAC GTCCCAGACC CTGGAGTGGG AGAAAGCGTC CCTCGTGGAC 
CAGCTGGCAG ACGCAGCGCG CCGCTACCAG CTCAAGACTG ACCAGTGCGA CAAACTTCAG 
GCCGCCCTGA GTCGCAGCCG CGACCACCCA CCATCCCCGA TTCGGCCCTC CGAGCCCCTC 
GAGTCTCGGC TGTCGAAGCT GGAGCTCGAG CGACAACTCT CGCAAGCCAA GGTCGAGAGG 
CTCAAGCTCA GGGTGACCGA GAGCCAGGCC GAAGTGAGGA GGCTGCAGGA AGCCAAGGAG 
GAAGCCACTT CCCAGCTGAC TCGGCTCGAG AGGAGGGCGG AGAGCCTCGA CCACGGCAAC 
AGCCGCCTGA GGGCAGAGAT TGCGGAGCAT GCGTCCAAGG TGAAGCTCCT GAACGCTCAC 
CTGGTGGAAG AGATTGCCAA GGGACGCGGC CTGGAGGAGG AGAAGCGATC TCTGGAAAGC 
CAGCTCAGGG TGGCCAGGGT GCAGCTCGAG TCTGCCAGGT TCGACCGGAA AAGAGATGGG 
TCGGTGATTG ACGAGCTGAA GGGCCAGAAG GGCACTCTGG AGACCAGGCT GTCTGCGCTC 
GCCGTACAGC TGGCAGAATG CGAGTCAGCC AAGCAGCGCC TCGACAAGAC GCCCATCAGG 
ACTCCCCACA GGGAAGAGAC CAGGACTTGG CAGCAGATGG TGGAGACGTT GCGCGAGGAG 
AAGCGGCAGC TCCAGGGGGA GGTGACGCGC CTCCGGCGGC AGCTCACGGG CACCATTGAC 
AGTATCCTGC CGCCGGCGAC TCCCGACGGG AGCCGAGCTT CGAGGCGGAC ACGCCGCAGT 
CTGGCAGAGC GTGACGTGCC AGACGGCGCA AGCCTCCATT CTCCCGAGGT GTCTCGCCTG 
AAGACGCAGA AGAGCCTCCT GGAGGCCAAA GTCCGCACTT TGGAGGAGCG CCTCTCTCAG 
CAAGACCAGG TGCACTCGGA GGAGCTGACG CGGGAACACC GGGAGCTGGT CGCCGAGATC 
GCCAGACTGC GGGAGGAGCT GGCAACGCAC GAGCGGCGCC GGACGTCGAC CCCCGTGCAC 
AACGAGCCTC CCGAGTTTGC CATCAATCTA AGCCACAGGC AAGACATCCA AGGGCTGCTC 
GACCGTTCCC TCCACACCGT GTCCGAAGAC CCGGAGCGGC TGCGCGACCA AGCCATGTTG 
CTCAGCGCGT TCATCGACGG AGCACTGGAA CCGGGGGACA GCCTTCGGAC CCCTTCTAAG 
CTTGCGCCAA ACAAGCGGAC CACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029851194.1
CDS	110..5056
Translation	MTYLGDGQVQAVQALGGSFQHSGGAVFESPLRIAVMEQPPENVIARLPSSSTINDEDYATAEEEPPGRTSRLEDDDQQGVLSDACSTDSDHELELVGLDREHELKSKLEKSRTMLRAYVQHTTNQDRLLGHLGAQVEEYRNRCSRLEEVLRQCPCADRTENIQDDRSYEEDPPDLPSALSALELERARCAELKQLNVVLREHLEITTETNATLTMHMERMTEDWQNATRADAVNRGSSSSATHDEFRADSEQGSVPRLRRDLQELKGDLGDMRQAFATSLASIQTMLHDAAEAAGRVQLSLVDDAADSDQPDLGLTSQENVDLRVREAMLQSEVRSHEATSKNLALELESVQKEKKECMGVIASLRKHVANLEDQQQVSERELSVLRKAVRDIEELNDSLEREMQEMCNASMQLKQSNSELAKAKSDMELAHEELAAERDSLARREADVTRAMARLESLLEESASRQTDLEATLSARDKEIAYRKAAFLQLSSQKDALSQEASSLRQSVASQRETNEQLSAQKEELATAKSQLEMELRHCRAEGDKLQSDLTGCKEAREATEVDLRNARNSLAKIQREKETTDAKLRDSAAELEKLHEKLKRLERERKADAEEWGMLRASLTEKLQAAEEAALRRVEEQRRLFKEEMDKHRQEKAGQLRQLEESHAAELKRQEELLSETETKWHSEVALHQQRLEDVRVELQEKLASSEQLRAEMQSMAEDERRELLNSLGRCKEEVSVLRRNLVAFRDEAGSKMAAEQAKVASALKDVDTLRVELEQTRTALRLKEAQEKSRESQFHKELSRVTEERERAIQRLNEVDASHAALQRELSETLGKKSEGERRVEVLTHQLSEARQSYTLVTAETEQLRRVIGELQQELRSATQERDASLQEASEKFRGAEKARATLAEELRELRNTHGGVERARQVLERKVRALESALLEHGSELDSLRKLSEQRGHQVESARQDVLSWKWKVAVLEEERDAWQRERASLGRRLDESERRAQAERNAREGAVREAGRVAERLAQDRADLEKQLLASGQATHEARLALCAAQGSTAALESELKVVSESRKSIEGTLDSLLSVLRCSLGIQTKYELRSPSPQRPAAGRRHGGDPSNESFRGDSSPFRSEDSLGRSLLRRPTPDVEAELVLQAVDALSHRIATLEADKEKVEAECERSQRVVAGLEDELTATKSALARLTKANDALVADNAELRSQVSSQLEAVSELKHTSQTLEWEKASLVDQLADAARRYQLKTDQCDKLQAALSRSRDHPPSPIRPSEPLESRLSKLELERQLSQAKVERLKLRVTESQAEVRRLQEAKEEATSQLTRLERRAESLDHGNSRLRAEIAEHASKVKLLNAHLVEEIAKGRGLEEEKRSLESQLRVARVQLESARFDRKRDGSVIDELKGQKGTLETRLSALAVQLAECESAKQRLDKTPIRTPHREETRTWQQMVETLREEKRQLQGEVTRLRRQLTGTIDSILPPATPDGSRASRRTRRSLAERDVPDGASLHSPEVSRLKTQKSLLEAKVRTLEERLSQQDQVHSEELTREHRELVAEIARLREELATHERRRTSTPVHNEPPEFAINLSHRQDIQGLLDRSLHTVSEDPERLRDQAMLLSAFIDGALEPGDSLRTPSKLAPNKRTT

Target ORF information:

RefSeq Version	XM_029995334.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis rootletin (LOC8029650), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029995334.1

ORF Insert Sequence:

ATGACATATC TTGGCGACGG GCAAGTTCAG GCGGTTCAGG CACTCGGTGG TTCATTCCAA 
CATTCTGGTG GAGCGGTATT TGAAAGCCCG CTTCGCATTG CAGTGATGGA GCAGCCACCG 
GAGAACGTAA TTGCGAGGCT ACCGTCTTCG TCGACCATCA ATGACGAGGA CTATGCAACG 
GCAGAGGAGG AACCACCAGG CAGAACCTCG AGGCTCGAGG ACGACGACCA GCAGGGCGTG 
CTCTCGGACG CCTGCTCGAC CGACAGCGAC CACGAGCTCG AGTTGGTGGG TCTGGATCGC 
GAACACGAGC TCAAGTCGAA GCTGGAGAAG TCGAGGACCA TGTTGCGGGC CTACGTGCAG 
CACACGACCA ATCAGGACAG GCTGCTTGGG CACCTGGGAG CACAGGTGGA GGAGTACCGG 
AACCGCTGCT CGAGATTGGA AGAGGTCCTC CGCCAGTGCC CGTGTGCCGA TCGAACTGAG 
AACATTCAAG ACGATCGCTC GTACGAAGAA GATCCTCCTG ACCTTCCGTC GGCCTTATCA 
GCGCTGGAAC TAGAGAGGGC AAGATGTGCG GAGCTGAAGC AGCTGAACGT GGTCCTCCGC 
GAGCACCTGG AGATAACGAC GGAGACGAAT GCGACCCTCA CCATGCACAT GGAGAGGATG 
ACGGAGGACT GGCAGAACGC GACCCGGGCT GACGCGGTCA ATCGCGGCAG CAGCAGCAGC 
GCCACCCACG ACGAGTTTCG CGCAGACTCC GAGCAGGGCA GCGTTCCCAG GCTGCGTCGA 
GACTTGCAGG AGCTGAAGGG GGACCTGGGA GACATGAGGC AGGCCTTTGC CACCAGCCTG 
GCCTCCATCC AGACGATGCT GCACGACGCC GCCGAGGCGG CCGGGCGTGT CCAACTCTCG 
CTTGTGGACG ACGCGGCGGA CAGCGACCAG CCAGACCTCG GGCTCACCAG CCAGGAAAAC 
GTGGACCTGC GCGTGCGGGA AGCCATGCTA CAGTCGGAGG TGCGCAGCCA CGAGGCCACG 
TCGAAGAATC TCGCCTTGGA GCTGGAGTCC GTCCAAAAGG AAAAGAAAGA GTGCATGGGT 
GTAATTGCGT CGCTGCGCAA GCATGTCGCC AACCTCGAGG ACCAGCAACA GGTGTCCGAA 
CGCGAGCTCA GCGTTCTGAG GAAAGCGGTG CGTGACATCG AAGAACTCAA CGACTCCCTG 
GAACGGGAGA TGCAAGAGAT GTGCAACGCC AGCATGCAGC TTAAACAGTC CAACTCGGAG 
CTGGCAAAGG CAAAGAGTGA CATGGAACTT GCGCACGAAG AGTTGGCCGC GGAACGGGAC 
TCCCTGGCAC GACGGGAAGC GGATGTAACG CGAGCGATGG CCCGTCTGGA GTCGCTCTTG 
GAAGAGAGCG CGTCTCGGCA GACGGATCTG GAGGCCACGC TCTCTGCCAG GGACAAGGAG 
ATCGCCTACA GGAAGGCGGC GTTTCTTCAG CTGTCCTCTC AGAAGGACGC GCTCTCGCAA 
GAAGCGTCCA GCCTTCGGCA GAGTGTCGCG AGCCAGCGGG AGACAAACGA GCAGCTCAGC 
GCACAGAAGG AAGAGCTGGC CACGGCCAAG TCTCAGCTGG AAATGGAGCT ACGGCACTGC 
CGGGCCGAGG GGGACAAGCT CCAGAGCGAC CTGACTGGCT GCAAGGAAGC CCGGGAAGCG 
ACAGAAGTGG ACCTGCGGAA CGCCAGGAAT TCCCTCGCAA AGATCCAGCG GGAGAAAGAG 
ACGACGGACG CCAAGCTTCG GGACTCAGCG GCAGAGCTGG AGAAATTGCA CGAGAAGCTG 
AAGAGGCTGG AAAGGGAGCG AAAAGCCGAT GCCGAGGAGT GGGGCATGCT GCGGGCATCC 
CTGACCGAGA AGCTGCAAGC TGCGGAGGAG GCGGCGTTGC GGAGGGTAGA AGAGCAACGG 
CGGCTGTTCA AGGAAGAGAT GGACAAACAC CGGCAGGAGA AGGCTGGCCA ATTGCGGCAG 
CTCGAGGAAA GCCACGCAGC AGAACTGAAG CGCCAGGAAG AGCTGCTGAG CGAGACAGAA 
ACCAAGTGGC ACAGCGAGGT GGCCCTGCAC CAGCAGAGGC TGGAAGACGT GAGGGTGGAG 
CTCCAGGAAA AGCTAGCTTC TTCCGAGCAG CTCAGAGCCG AGATGCAGTC TATGGCGGAG 
GACGAGCGCA GGGAGCTGCT GAACTCTCTC GGCCGCTGCA AGGAGGAAGT GTCCGTGCTC 
CGGCGAAACC TGGTGGCATT CAGGGATGAA GCAGGCTCCA AGATGGCCGC CGAGCAGGCC 
AAGGTGGCGT CTGCTCTGAA GGACGTCGAC ACGCTTCGCG TGGAACTGGA GCAGACTAGG 
ACGGCGTTGC GTTTGAAAGA GGCACAGGAA AAGTCACGTG AGTCGCAGTT TCACAAGGAA 
CTCAGCCGGG TGACCGAAGA AAGGGAGCGT GCCATTCAGC GGCTGAACGA GGTCGATGCC 
AGTCATGCGG CTCTTCAGCG CGAGCTGTCT GAAACTCTTG GCAAGAAATC CGAAGGGGAG 
CGTCGAGTCG AAGTTCTGAC TCACCAGTTG TCCGAGGCAC GTCAGAGTTA CACGTTGGTC 
ACGGCAGAGA CCGAGCAGCT TCGCCGAGTC ATTGGGGAGC TGCAGCAGGA GTTGCGCAGC 
GCCACTCAGG AGAGGGACGC CTCGCTTCAG GAGGCTTCCG AAAAATTCCG CGGCGCGGAG 
AAAGCGCGGG CGACGTTGGC GGAGGAGCTT CGCGAGCTCC GGAACACGCA CGGCGGCGTA 
GAGCGGGCTC GGCAGGTCTT GGAGCGCAAG GTGCGAGCCC TGGAGTCGGC TTTGCTGGAG 
CACGGCTCGG AGCTGGACTC GCTCCGGAAG CTGTCCGAGC AGAGGGGACA TCAGGTGGAA 
TCGGCACGTC AGGACGTTCT CTCCTGGAAG TGGAAGGTTG CCGTGCTCGA GGAAGAGCGG 
GACGCGTGGC AGCGCGAGCG TGCCTCCTTG GGCCGTCGTC TGGACGAGAG CGAGCGCCGG 
GCACAGGCGG AGCGCAACGC TCGTGAAGGG GCGGTGCGCG AGGCCGGCCG GGTTGCCGAG 
AGACTGGCCC AGGACCGGGC CGACCTGGAG AAGCAGCTGC TGGCATCCGG GCAAGCAACG 
CACGAGGCAC GCCTGGCACT GTGCGCCGCG CAGGGCTCCA CGGCTGCCCT CGAGAGCGAG 
CTCAAGGTCG TCTCCGAGTC TCGGAAGTCG ATCGAGGGCA CTCTGGACTC GCTGCTGAGC 
GTGCTGCGCT GCTCCCTGGG GATCCAGACC AAATACGAGC TGCGGTCCCC CTCCCCCCAG 
CGACCGGCGG CGGGCCGGCG CCACGGCGGC GATCCGTCCA ACGAGAGCTT TCGGGGGGAC 
AGCTCGCCCT TTCGGTCGGA GGACTCCCTC GGCCGCTCCC TGCTGCGGCG GCCCACCCCC 
GACGTCGAGG CCGAGCTCGT GCTACAAGCC GTGGACGCCC TGTCGCACCG CATCGCCACG 
CTTGAAGCCG ACAAGGAGAA GGTGGAGGCT GAGTGCGAGC GTTCCCAGCG GGTGGTTGCC 
GGACTGGAGG ACGAGCTCAC GGCCACAAAG TCCGCGCTTG CGCGACTGAC CAAGGCAAAC 
GATGCTCTCG TTGCAGACAA TGCGGAGCTG CGCTCGCAAG TGTCCTCTCA GCTGGAGGCG 
GTCTCGGAGC TCAAACACAC GTCCCAGACC CTGGAGTGGG AGAAAGCGTC CCTCGTGGAC 
CAGCTGGCAG ACGCAGCGCG CCGCTACCAG CTCAAGACTG ACCAGTGCGA CAAACTTCAG 
GCCGCCCTGA GTCGCAGCCG CGACCACCCA CCATCCCCGA TTCGGCCCTC CGAGCCCCTC 
GAGTCTCGGC TGTCGAAGCT GGAGCTCGAG CGACAACTCT CGCAAGCCAA GGTCGAGAGG 
CTCAAGCTCA GGGTGACCGA GAGCCAGGCC GAAGTGAGGA GGCTGCAGGA AGCCAAGGAG 
GAAGCCACTT CCCAGCTGAC TCGGCTCGAG AGGAGGGCGG AGAGCCTCGA CCACGGCAAC 
AGCCGCCTGA GGGCAGAGAT TGCGGAGCAT GCGTCCAAGG TGAAGCTCCT GAACGCTCAC 
CTGGTGGAAG AGATTGCCAA GGGACGCGGC CTGGAGGAGG AGAAGCGATC TCTGGAAAGC 
CAGCTCAGGG TGGCCAGGGT GCAGCTCGAG TCTGCCAGGT TCGACCGGAA AAGAGATGGG 
TCGGTGATTG ACGAGCTGAA GGGCCAGAAG GGCACTCTGG AGACCAGGCT GTCTGCGCTC 
GCCGTACAGC TGGCAGAATG CGAGTCAGCC AAGCAGCGCC TCGACAAGAC GCCCATCAGG 
ACTCCCCACA GGGAAGAGAC CAGGACTTGG CAGCAGATGG TGGAGACGTT GCGCGAGGAG 
AAGCGGCAGC TCCAGGGGGA GGTGACGCGC CTCCGGCGGC AGCTCACGGG CACCATTGAC 
AGTATCCTGC CGCCGGCGAC TCCCGACGGG AGCCGAGCTT CGAGGCGGAC ACGCCGCAGT 
CTGGCAGAGC GTGACGTGCC AGACGGCGCA AGCCTCCATT CTCCCGAGGT GTCTCGCCTG 
AAGACGCAGA AGAGCCTCCT GGAGGCCAAA GTCCGCACTT TGGAGGAGCG CCTCTCTCAG 
CAAGACCAGG TGCACTCGGA GGAGCTGACG CGGGAACACC GGGAGCTGGT CGCCGAGATC 
GCCAGACTGC GGGAGGAGCT GGCAACGCAC GAGCGGCGCC GGACGTCGAC CCCCGTGCAC 
AACGAGCCTC CCGAGTTTGC CATCAATCTA AGCCACAGGC AAGACATCCA AGGGCTGCTC 
GACCGTTCCC TCCACACCGT GTCCGAAGAC CCGGAGCGGC TGCGCGACCA AGCCATGTTG 
CTCAGCGCGT TCATCGACGG AGCACTGGAA CCGGGGGACA GCCTTCGGAC CCCTTCTAAG 
CTTGCGCCAA ACAAGCGGAC CACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC8029650 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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