LOC8037553 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC8037553
Entrez Gene ID	8037553
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Ixodes scapularis(black-legged tick)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029973180.1	XP_029829040.1	spatacsin isoform X2
XM_002412352.1	XP_002412397.1	hypothetical protein
XM_029973181.1	XP_029829041.1	spatacsin isoform X2
XM_029973179.1	XP_029829039.1	spatacsin isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC8037553 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC8037553 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OIa08191	XM_002412352.1 Latest version!	Ixodes scapularis hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OIa42604	XM_029973181.1 Latest version!	Ixodes scapularis spatacsin (LOC8037553), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIa42604	XM_029973180.1 Latest version!	Ixodes scapularis spatacsin (LOC8037553), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIa42603	XM_029973179.1 Latest version!	Ixodes scapularis spatacsin (LOC8037553), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC8037553 ( XM_002412352.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_002412397.1 Ixodes scapularis hypothetical protein, mRNA.

Price & Availability↑

CloneID	OIa08191
Clone ID Related Accession (Same CDS sequence)	XM_002412352.1
Accession Version	XM_002412352.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2009-06-22
Organism	Ixodes scapularis(black-legged tick)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002778756). COMPLETENESS: incomplete on both ends.

ATGCAAGAGC TTGGAGGCGA CAATTGGCGT CTACTTCTGG AGCACGGCCA TCTGGAGCGT 
GCCCTGTCGG GTCCACTTCC CAGCGAAGCT CTTGAGGCGT CCTCCTGCAT GTCGCACGTG 
CATCGTCTGC TGTTGGCTGC GCTGGCAAGG GGTGGCCAGA TACACGAGGA CGTTCTGGAG 
GACTTTGGGG ACCTTTTGGG TCTCATGGGG GAGTCTGGCC TGAAGCCCTC ACAGCTGGCC 
AGGGTCTGCC ACGGCCCCGT GACCCCCGAG GACTTTGTCC ACCGCATGGT GCACTACTGC 
CTCCGGGAAA GGCTGCACCA CTTCCTGTAC CTGTTTCTCA CGGACCTCAG GCCGGAGCTT 
CCTCCACTGT GCCCCCAGTG CAACGGCTGC TTTGTCCAGG CACTTCGGGA TCACTGGCAG 
TGGCTGGACG ACCCACAAGA TGTTGCGAAG GCAGAGCGTG CCATTGTATC CTTGGCTCGA 
AGCCTGCCAG CAAAGGGCAG CGCAGTGCCG AGGATATTGG AGCCGGCTGT TGTGGGTGCC 
GACCTCCAGG ACATCTCTCT ATACCAGCTG CTGCAGAAAG GAACCCCCTT TGAGATAGCG 
GGCCTGTTTG CCTGGCAGAC CACCAACGCC TGTCAGGTGG ACTCTGGCGG GTCGCTGATG 
CCCCACTTCT CCCTGCCGTC CCTGCGCGAC AAGTTTGGGC TGCGGGAGGA GCTCAGCTTC 
CCCTACTACC TCCACAAGGG GCGCCCCTGT GCGGCTGCCT TCAAGTTCCT CGCGCAGGAG 
CTCCTTCTGC ACGGATCTCT CTCCACCAAA AGGATGGACG AGAAGTGCAG CAGCGGAATC 
TTGCTGCTTT TACGGTTTGC AACTGAGCAC GGGTTTGAGG TGCCCGACGT CGCGCTGCGA 
TTGTGCGCAG CTGCGAACGA CTGGCTCGGC TTTCTGCTCT GGGCACAGCT CCTTCAGTGC 
CCCAGGACAC AGGTGCTGGA TCTAGTGCAG CTCTTCCAAG ATGGCTGCCT CCGTGAGCAC 
TTGAGCAAGG CGCTTTCAGT AGTGAGCTCC CTGCTGCCGA GCAAGCATCA CCGCCGGGAC 
CTCCGGGCAT CTCTTTATGC ACGCATTGGA CTTCAGGATC CCTCCACTAG GGGGCACGAC 
CCTTCAGAAC CGCACTACTC GACGGACCTC GTTGAGCTGA TGCTGCAGTG CCATTGCGGC 
AGCGGAACTA CCCCCGGGTC TTCGGGCATG GCCTGGCAGA GCCTGCTGCG TGCTGCGGTG 
GTCCTGAAGC ACCAGACACC CTGCGTCATT GCCGGCTGCT GCCCCGATGC CCCCCTGGGC 
AGCTGCCTGG CATTGTGGCT CAAACTTTGT TCTGGCGGAG AGGAAGCGGA GATGCCCGAG 
GACGGCCGCC TCGAATTCAC CCTGGCGGAG TTGCAGCAGA CGATGGAGGC GGTGATCAAG 
AGGGGCCGAC TGCGGACTCT TCTCCTGGGA CTCCAGCTCT TCCAGCCTAT GGAAGCCGCT 
TCAACACCAG GGGTCGGCCT GACCTCTCTG CCTTGGCTAC GTGAGACCTC CCAGCGTCTG 
TTGGGACGCG CTTTTGGCCT GTGCCGTTCC CACCTCGACC TGGCAGCCCT CGCGGCCTTC 
TGCGACGAGC TTCACGGGGG CGATAAGATG CCGCACCTCC ACAAACTCCT CGCATCTCTG 
AGTCCGGCGT CCCTGCTTCA TGAGTTTGAC TGGAGCTCGT TGTGGGGCCA ACCGGGTGAA 
AGGGCAGCCA CCCTGGCACG ACTGGTGTCG CAGCTCAACC AGACGGGCCG TTTCTCGGAG 
GCGCTGGCGC TGGCTAGGGC GGCGGGACTG CCACTAGCGG ACGTGGCGCT CCAGCAGCTG 
GAACTGAGGT TCGAGGATCG CTCTGCCACC GCTAATGACA CATTCTGGGA GGATTGCGAC 
CAAGTGCTTC AAGAGAACTT CGTGGATCCT GCACTCGCCT TCGACTTTCT CAAGGGCAAA 
ACGCTGCAAG TGGAAGGTGC GCGGGAACTT TACGAGGTGG GCCACCGTGC GCTGCAGTGG 
CTGCGCCGCA GCGCTGCCTG GGGCTCGCCC GAGGCCCAGC GCTGGGAGGC GCGGGTGTGG 
CTATGGTGTG TGCGCAGCGG GGCAGAGCCC ACCCTGGAAG CGAACCCCAA GCCAGACCCC 
CTGGTGGCAC AGCCCCCTCC CAGCCCGGGG GCCTGCGTCA CCCTGGACGA CTCGCAGGTG 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002412397.1
CDS	1..2226
Translation	MQELGGDNWRLLLEHGHLERALSGPLPSEALEASSCMSHVHRLLLAALARGGQIHEDVLEDFGDLLGLMGESGLKPSQLARVCHGPVTPEDFVHRMVHYCLRERLHHFLYLFLTDLRPELPPLCPQCNGCFVQALRDHWQWLDDPQDVAKAERAIVSLARSLPAKGSAVPRILEPAVVGADLQDISLYQLLQKGTPFEIAGLFAWQTTNACQVDSGGSLMPHFSLPSLRDKFGLREELSFPYYLHKGRPCAAAFKFLAQELLLHGSLSTKRMDEKCSSGILLLLRFATEHGFEVPDVALRLCAAANDWLGFLLWAQLLQCPRTQVLDLVQLFQDGCLREHLSKALSVVSSLLPSKHHRRDLRASLYARIGLQDPSTRGHDPSEPHYSTDLVELMLQCHCGSGTTPGSSGMAWQSLLRAAVVLKHQTPCVIAGCCPDAPLGSCLALWLKLCSGGEEAEMPEDGRLEFTLAELQQTMEAVIKRGRLRTLLLGLQLFQPMEAASTPGVGLTSLPWLRETSQRLLGRAFGLCRSHLDLAALAAFCDELHGGDKMPHLHKLLASLSPASLLHEFDWSSLWGQPGERAATLARLVSQLNQTGRFSEALALARAAGLPLADVALQQLELRFEDRSATANDTFWEDCDQVLQENFVDPALAFDFLKGKTLQVEGARELYEVGHRALQWLRRSAAWGSPEAQRWEARVWLWCVRSGAEPTLEANPKPDPLVAQPPPSPGACVTLDDSQVQ

Target ORF information:

RefSeq Version	XM_002412352.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002412352.1

ORF Insert Sequence:

ATGCAAGAGC TTGGAGGCGA CAATTGGCGT CTACTTCTGG AGCACGGCCA TCTGGAGCGT 
GCCCTGTCGG GTCCACTTCC CAGCGAAGCT CTTGAGGCGT CCTCCTGCAT GTCGCACGTG 
CATCGTCTGC TGTTGGCTGC GCTGGCAAGG GGTGGCCAGA TACACGAGGA CGTTCTGGAG 
GACTTTGGGG ACCTTTTGGG TCTCATGGGG GAGTCTGGCC TGAAGCCCTC ACAGCTGGCC 
AGGGTCTGCC ACGGCCCCGT GACCCCCGAG GACTTTGTCC ACCGCATGGT GCACTACTGC 
CTCCGGGAAA GGCTGCACCA CTTCCTGTAC CTGTTTCTCA CGGACCTCAG GCCGGAGCTT 
CCTCCACTGT GCCCCCAGTG CAACGGCTGC TTTGTCCAGG CACTTCGGGA TCACTGGCAG 
TGGCTGGACG ACCCACAAGA TGTTGCGAAG GCAGAGCGTG CCATTGTATC CTTGGCTCGA 
AGCCTGCCAG CAAAGGGCAG CGCAGTGCCG AGGATATTGG AGCCGGCTGT TGTGGGTGCC 
GACCTCCAGG ACATCTCTCT ATACCAGCTG CTGCAGAAAG GAACCCCCTT TGAGATAGCG 
GGCCTGTTTG CCTGGCAGAC CACCAACGCC TGTCAGGTGG ACTCTGGCGG GTCGCTGATG 
CCCCACTTCT CCCTGCCGTC CCTGCGCGAC AAGTTTGGGC TGCGGGAGGA GCTCAGCTTC 
CCCTACTACC TCCACAAGGG GCGCCCCTGT GCGGCTGCCT TCAAGTTCCT CGCGCAGGAG 
CTCCTTCTGC ACGGATCTCT CTCCACCAAA AGGATGGACG AGAAGTGCAG CAGCGGAATC 
TTGCTGCTTT TACGGTTTGC AACTGAGCAC GGGTTTGAGG TGCCCGACGT CGCGCTGCGA 
TTGTGCGCAG CTGCGAACGA CTGGCTCGGC TTTCTGCTCT GGGCACAGCT CCTTCAGTGC 
CCCAGGACAC AGGTGCTGGA TCTAGTGCAG CTCTTCCAAG ATGGCTGCCT CCGTGAGCAC 
TTGAGCAAGG CGCTTTCAGT AGTGAGCTCC CTGCTGCCGA GCAAGCATCA CCGCCGGGAC 
CTCCGGGCAT CTCTTTATGC ACGCATTGGA CTTCAGGATC CCTCCACTAG GGGGCACGAC 
CCTTCAGAAC CGCACTACTC GACGGACCTC GTTGAGCTGA TGCTGCAGTG CCATTGCGGC 
AGCGGAACTA CCCCCGGGTC TTCGGGCATG GCCTGGCAGA GCCTGCTGCG TGCTGCGGTG 
GTCCTGAAGC ACCAGACACC CTGCGTCATT GCCGGCTGCT GCCCCGATGC CCCCCTGGGC 
AGCTGCCTGG CATTGTGGCT CAAACTTTGT TCTGGCGGAG AGGAAGCGGA GATGCCCGAG 
GACGGCCGCC TCGAATTCAC CCTGGCGGAG TTGCAGCAGA CGATGGAGGC GGTGATCAAG 
AGGGGCCGAC TGCGGACTCT TCTCCTGGGA CTCCAGCTCT TCCAGCCTAT GGAAGCCGCT 
TCAACACCAG GGGTCGGCCT GACCTCTCTG CCTTGGCTAC GTGAGACCTC CCAGCGTCTG 
TTGGGACGCG CTTTTGGCCT GTGCCGTTCC CACCTCGACC TGGCAGCCCT CGCGGCCTTC 
TGCGACGAGC TTCACGGGGG CGATAAGATG CCGCACCTCC ACAAACTCCT CGCATCTCTG 
AGTCCGGCGT CCCTGCTTCA TGAGTTTGAC TGGAGCTCGT TGTGGGGCCA ACCGGGTGAA 
AGGGCAGCCA CCCTGGCACG ACTGGTGTCG CAGCTCAACC AGACGGGCCG TTTCTCGGAG 
GCGCTGGCGC TGGCTAGGGC GGCGGGACTG CCACTAGCGG ACGTGGCGCT CCAGCAGCTG 
GAACTGAGGT TCGAGGATCG CTCTGCCACC GCTAATGACA CATTCTGGGA GGATTGCGAC 
CAAGTGCTTC AAGAGAACTT CGTGGATCCT GCACTCGCCT TCGACTTTCT CAAGGGCAAA 
ACGCTGCAAG TGGAAGGTGC GCGGGAACTT TACGAGGTGG GCCACCGTGC GCTGCAGTGG 
CTGCGCCGCA GCGCTGCCTG GGGCTCGCCC GAGGCCCAGC GCTGGGAGGC GCGGGTGTGG 
CTATGGTGTG TGCGCAGCGG GGCAGAGCCC ACCCTGGAAG CGAACCCCAA GCCAGACCCC 
CTGGTGGCAC AGCCCCCTCC CAGCCCGGGG GCCTGCGTCA CCCTGGACGA CTCGCAGGTG 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8037553 ( XM_029973180.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029829040.1 Ixodes scapularis spatacsin (LOC8037553), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OIa42604
Clone ID Related Accession (Same CDS sequence)	XM_029973180.1 , XM_029973181.1
Accession Version	XM_029973180.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	spatacsin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021924022.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGGT GCGTGCACCA AGGGAACATT CCGGAGGGCC AGTACCACCT GCTGCGTCGC 
AGGGCGGGCT CCGTCGAGCC CCTGCTGAGC ACCTTTCAGG AGGTTGCTTT GAGGGAGGCA 
TTGTGCCGGC TCCGTGAGGA TGACTTTCCC GAGGCGGAAT GGCTCTTGAC AAACGTGGGG 
TTCTTTGCCA GAGTTAAGAT GCGGGAGCTG CTCCTCCTGT CGCGCGACCG GCGGATTCGG 
CGGGTGCTGA GACGGGAGCT GGTGAGGATG GCGGTCCTGA CCGAGGCGGA CTCCAGTCTT 
CTCGACAAAG TGGCGGCCAT CGAAGAGTGT TTTCCCCAGG AAGATGTGGG ACAGGCATCA 
TGGTTGGCCT CGCAGAAGCG AGCGCAGCTT AGGTTGGTCA CTCCACAGGC TTCTGAGCTT 
GCTGTGGCGG TACTGAAACC GGCGGAGTTG CCACCAAGGC AGGCACCGAA GGCAGGTGGC 
GACACTTGGC TCCTGGACGA GTCGCCCTAT TTCCGAGTGG CGCCTGCCTG GCTTCATTAC 
TGGGACGCCG ACACCTTAGA CAGAGTTTTG GCCGAGGGGC GTGCCGGCTC GCCTCTCCTG 
GATATGCAAG AGCTTGGAGG CGACAATTGG CGTCTACTTC TGGAGCACGG CCATCTGGAG 
CGTGCCCTGT CGGGTCCACT TCCCAGCGAA GCTCTTGAGG CGTCCTCCTG CATGTCCCAC 
GTGCATCGTC TGCTGTTGGC TGCACTGGCA AGGGGTGGCC AGATACATGA GGACGTTCTG 
GAGGACTTTG GGGACCTCCT GGGTCTCATG GGGGAGTCTG GCCTGAAGCC CTCGCAGCTG 
GCCAGGGTCT GCCACGGCCC CGTGACCCCC GAGGACTTTG TCCATCGCAT GGTGCACTAC 
TGCCTCCGGG AGAGGCTGCA CCACTTCCTG TACCTGTTTC TCACGGACCT CAGGCCAGAG 
CTTCCTCCAC TGTGCCCCCA GTGCAACGGC TGCTTTGTCC AGGCACTTCG GGATCATTGG 
CAGTGGCTGG ACGACCCACA AGATGTTGCA AAGGCAGAGC GTGCCATTGC ATCCTTGGCT 
CGAAGCCTGC CAGCAAAGGG CAGCGCAGTG CCGAGGATAT TGGAGCCGGC TGTTGTGGGT 
GCCGACCTCC AGGACATCTC TCTGTACCAG CTGCTGCAGA AAGGAACCCC CTTTGAGATA 
GCGGGCCTGT TTGCCTGGCA GACTACCAAC GCCTGTCAGG TGGACTCTGG CGGGTCGCTG 
ATGCCCCACT TCTCCCTGCC GTCCCTGCGC GACAAGTTTG GGCTGCGGGA GGAGCTGAGC 
TTCCCCTACT ACCTCCACAA GGGGCGCCCC TGTGCGGCTG CCTTCAAGTT CCTCGCGCAG 
GAGCTCCTGC TGCACGGATC TCTCTCCACC AAAAGGATAC GCTCAGCCTG TCATCGAGCT 
TTGCGGTTGG CGGAACAACG CCCCCACGAC GCCCGTGCCG TTGCTGCGAG CGTCGCTTTT 
GCGGAGATTC TGGGCATGGA TACTCGCAGC TTCCGAGTCA CCCTCCGGAC AACTCGGCTG 
GCCTTGACAT CTTCTCCTGG CTGCCAAAGT CGACTCAGCG ACATCCTGAG AGTGCTTCGT 
CGAAGCAAGG ACGCCATGCA GGAAGTCCTG CTGCAGGCAA CTCTTGCCTT AGAAGAACAG 
CTGACGGGTG CTGCGTCCTT CTCACCGATG GACGAGAAGT GCAGCAGCGG AATCTTGCTG 
CTTTTACGGT TTGCAACTGA GCACGGGTTT GAGGTGCCCG ACGTCGCGCT GCGATTGTGC 
GCAGCTGCGA ACGACTGGCT CGGTTTTCTG CTCTGGGCAC AGCTCCTTCA GTGCCCCAGG 
ACACAGGTGC TGGATCTAGT GCAGCTCTTC CAAGATGGCT GCCTCCGTGA GCACTTGAGC 
AAGGCGCTTT CACTAGTGAG CTCCCTGCAG CCGAGCAAGC ATCACCGCCG GGACCTCCGG 
GCATCTCTTT ATGTGCGCAT TGGACTTCAG GATCCCTCCA CCAGGGGGCA CGACCCTTCA 
GAATCGGCGG CTGAACAAGA TGCTGTGTCA GTGTGCAGCA GTGAAGGCAT CGACGCTGCT 
GCTGCCCTTA ACACCGAACC GCACTACTCG ACGGACCTCG TTGAGCTGAT GCTGCAGTGC 
CATTGCGGCG GCGGGGCTAC CCCCGGGTCT TCGGGCATGG CCTGGCAGAG CCTGCTGCGT 
GCTGCGGTGG TCCTGAAGCA CCAGACGCCC TGCGTCATTG CCGGCTGCTG TCCCGATGCC 
CCCCTGGGCA GCTGCCTGGC ATTGTGGCTC AAACTTTGTT CCGGCGGAGA GGAAGCGGAA 
ATGCCCGAGG ACGGTCGCCT TGAATTCACC CTGGCGGAGC TGCAGCAGAC GATGGAGGTG 
GTGGTCAAGA GGGGCCGACT GCGGACTCTT CTCCTGGGAC TCCAGCTCTT CCAGCCTAGA 
AGCCCTCTAG TCACACTCGT CGAGTTCCTG GTCGCATTCT TTCTGGAGCG GAACCGAAAA 
CGAGCCGAGG AATGCCTTGA GCGCCTCAAG CGCAAGTTCA CCAAGATGGA AGCCGCTTCA 
ACACCAGGGG TCGGCCTGAC CTCTCTGCCT TGGCTACGTG AGACCTCCCA GCGTCTGTTG 
GGACGCGCTT TTGGCCTGTG CCGTTCCCAC CTCGACCTGG CAGCCCTCGC GGCCTTCTGC 
GACAAGCTTC ACGGGGGCGA TAAGATGCCG CACCTCCACA AACTCCTCGC ATCTTTGAGT 
CCGGCGTCCC TGCTTCATGA GTTTGACTGG AGCTCGTTGT GGGGCCAACC GGGTGAAAGG 
GCGGCCACCC TGGCACGACT GGTGTCGCAG CTCAACCAGA CGGGCCGTTT CTCGGAGGCG 
CTGGCGCTGG CTAGGGCGGC GGGACTGCCA CTAGCGGACG TGGCGCTCCA GCAGCTGGAA 
CTGAGGTTCG AGGATCGCTC TGCCACCGCT AATGACACAT TCTGGGAGGA TTGCGACCAA 
GTGCTTCAGG AGAACTCCGT GGATCCTGCA CTCGCCTTCG ACTTTCTCAA GGGCAAAACG 
CTGCAAGTGG AAGGTGCACG GGAACTGTAC GAGGTGGGCC ACCGTGCGCT GCAGTGGCTG 
CGCCGCAGCG CTGCCTGGGG CTCGCCCGAG GCCCAGCGCT GGGAGGCGCG GGTGTGGCTA 
TGGTGTGTGC GCAGCGGGGC AGAGCCCACC CTCGAAGCGA ACCCCAAGCC AGACCCCCTG 
GTGGCACAGC CCCCTCCCAG CCCGGGGGCC TGCGTCACCC TGGACGACTC GCAGCAAGTG 
GCGGCGACGG AAAAGTTAAT TGACGAGCTG CTGTCTCGGG GCCACTTCTG CAAGGCTTGC 
CAGCTGGCTT TCCTGTTCGG CTGCTCCACA AGCGACCTGG AACTCTTGCT GACATGCATC 
CGTCTAGCAC AAGGAACGCA GTCGTCCGAC AACCTTGATC CACGAATCCA ACTTCGGATC 
AGCGAACCAG CACCAGTTCT GGCCCACAGG CTTTCAGTGG TTGCATCTAT GTCTGCTGTG 
GACTTGAAGT CCTTGGAAGG TGGCGAGGAC TCACCGGAGA GAAAGACACT CCAACTCCTG 
GAGCGCATCA CCAGAGCAGC GAAGAAGAGC CAGCAGCTGT GTCAGCGTGT GCTGCTCACC 
TTCACCCTTT CACTGAACCT GGGGTGCAGC TACTCGGTCC TGGCCCTGGA GTCGGACCCC 
GTGGCCCTTT TGGGCAACCT GGTGGGCCAT AGCCTGGCCA AGATGGAGGT CCAGAAGCGG 
GCGTCTGGCC AGAGGGACGG CGCCCGCCAG TGCTGCTCTG CGGACTTTGC CCTGGCCAAG 
AAACTGGTGG CCGTCTTCGG GATCCCGGAC GACCGGGTGG CAAACTTTCT CTTCCACATG 
GCCATGGACG CTATCCGAGG AAACGCAGTT GCAAGTAAAG CGGGCATCTT GGAGGTGTGG 
GACTTGGCCT TGGAGCTCTG CCCCGACCCT TCGCTACTGG GGAACCTTTT GCTCAGGGCT 
AGGGTTCACG ACCTGCGCGC TCTGAGCAGC AACCCCAAAG CGCTGTCTGT GGAGGTGGAG 
CTGTGCGTGC GCGCCCACAG CTGCTTCCTG GAGGCGTGCA GCATGGAGGG CATCTCGCGG 
GTGCTCCACC GCTGCCACCG TCTCACGCCC TGCTTGGTGG CGGGGCGGCA CTTCTCGCTG 
CTCGTGTCTT TGCTCACTGG CATGGCGCGC TACAGCGAGA TGGCCTACGT CTTTGACCTG 
CTCCTGCAGA ACCACCACTT TGAGCTGCTC TTCCAGCGGG GCATGGACAA GGTGCCTTAC 
CTGCGGGTGG CGCTTCTAGA CTACCTGAAA CACCGCGCCA GCACCGACCC AGATCTCTAC 
TCGATGCTCA CCCTCAACTT CAACATGCAT CGCGAGATTG CCGAGAGCCT CGAACTGACT 
GCACTGACCA AGATGAAGAA GCTCGTCACG GACGGACCAA TGGCATGGAG TCCCCAAGAG 
CAGCGGGCGC TGGAAACGGT GCTTCAAGAC CTGGCAGACG CAGCCGAGAG CTACGTCAAG 
GCGGAGTGCT TGCTGCGGGC GCAGTCGTGC GGCAGGAAGG CACAGCTGGT TGCCCTGCAG 
CTACGCTACT TCGCGTCACA GCTGGTGCTC ATCAACCTGG AGCCGTCAGC CGCCATGACG 
CAGGTGGCAC GTCACCCAAA CTTCTTTGAG GCACACATAG TGGCGGAGGC GTACGGCCTG 
CAGGGCTGGC ACAGCGCGGC CCTCTTCAGC AGGGTGCTCC TGGACGGCGA CTGGGGCTAC 
CTGGCCGACT TCTGCTCCGT CTGCGAGCTC ACCTCTCAGC ACGCACACGA GCTTGCACTC 
AGGTACCAGA ACGAAGCGGC CGGAAACGCA AAATGTCGGG ATGCGCTCGA GAAGCTCCTG 
GAACGTCTCC CGTGCGTGCT GTCTCGTCTG CAGCTGGCCC AGAAACTAGG CTTTGCACGC 
CTTGCGTCGC AGACTCTCGA GGCACACCCT TACCTTCGAG ACTACCTCGA CCAGCGGACG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029829040.1
CDS	30..5072
Translation	MLRCVHQGNIPEGQYHLLRRRAGSVEPLLSTFQEVALREALCRLREDDFPEAEWLLTNVGFFARVKMRELLLLSRDRRIRRVLRRELVRMAVLTEADSSLLDKVAAIEECFPQEDVGQASWLASQKRAQLRLVTPQASELAVAVLKPAELPPRQAPKAGGDTWLLDESPYFRVAPAWLHYWDADTLDRVLAEGRAGSPLLDMQELGGDNWRLLLEHGHLERALSGPLPSEALEASSCMSHVHRLLLAALARGGQIHEDVLEDFGDLLGLMGESGLKPSQLARVCHGPVTPEDFVHRMVHYCLRERLHHFLYLFLTDLRPELPPLCPQCNGCFVQALRDHWQWLDDPQDVAKAERAIASLARSLPAKGSAVPRILEPAVVGADLQDISLYQLLQKGTPFEIAGLFAWQTTNACQVDSGGSLMPHFSLPSLRDKFGLREELSFPYYLHKGRPCAAAFKFLAQELLLHGSLSTKRIRSACHRALRLAEQRPHDARAVAASVAFAEILGMDTRSFRVTLRTTRLALTSSPGCQSRLSDILRVLRRSKDAMQEVLLQATLALEEQLTGAASFSPMDEKCSSGILLLLRFATEHGFEVPDVALRLCAAANDWLGFLLWAQLLQCPRTQVLDLVQLFQDGCLREHLSKALSLVSSLQPSKHHRRDLRASLYVRIGLQDPSTRGHDPSESAAEQDAVSVCSSEGIDAAAALNTEPHYSTDLVELMLQCHCGGGATPGSSGMAWQSLLRAAVVLKHQTPCVIAGCCPDAPLGSCLALWLKLCSGGEEAEMPEDGRLEFTLAELQQTMEVVVKRGRLRTLLLGLQLFQPRSPLVTLVEFLVAFFLERNRKRAEECLERLKRKFTKMEAASTPGVGLTSLPWLRETSQRLLGRAFGLCRSHLDLAALAAFCDKLHGGDKMPHLHKLLASLSPASLLHEFDWSSLWGQPGERAATLARLVSQLNQTGRFSEALALARAAGLPLADVALQQLELRFEDRSATANDTFWEDCDQVLQENSVDPALAFDFLKGKTLQVEGARELYEVGHRALQWLRRSAAWGSPEAQRWEARVWLWCVRSGAEPTLEANPKPDPLVAQPPPSPGACVTLDDSQQVAATEKLIDELLSRGHFCKACQLAFLFGCSTSDLELLLTCIRLAQGTQSSDNLDPRIQLRISEPAPVLAHRLSVVASMSAVDLKSLEGGEDSPERKTLQLLERITRAAKKSQQLCQRVLLTFTLSLNLGCSYSVLALESDPVALLGNLVGHSLAKMEVQKRASGQRDGARQCCSADFALAKKLVAVFGIPDDRVANFLFHMAMDAIRGNAVASKAGILEVWDLALELCPDPSLLGNLLLRARVHDLRALSSNPKALSVEVELCVRAHSCFLEACSMEGISRVLHRCHRLTPCLVAGRHFSLLVSLLTGMARYSEMAYVFDLLLQNHHFELLFQRGMDKVPYLRVALLDYLKHRASTDPDLYSMLTLNFNMHREIAESLELTALTKMKKLVTDGPMAWSPQEQRALETVLQDLADAAESYVKAECLLRAQSCGRKAQLVALQLRYFASQLVLINLEPSAAMTQVARHPNFFEAHIVAEAYGLQGWHSAALFSRVLLDGDWGYLADFCSVCELTSQHAHELALRYQNEAAGNAKCRDALEKLLERLPCVLSRLQLAQKLGFARLASQTLEAHPYLRDYLDQRT

Target ORF information:

RefSeq Version	XM_029973180.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis spatacsin (LOC8037553), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029973180.1

ORF Insert Sequence:

ATGCTGCGGT GCGTGCACCA AGGGAACATT CCGGAGGGCC AGTACCACCT GCTGCGTCGC 
AGGGCGGGCT CCGTCGAGCC CCTGCTGAGC ACCTTTCAGG AGGTTGCTTT GAGGGAGGCA 
TTGTGCCGGC TCCGTGAGGA TGACTTTCCC GAGGCGGAAT GGCTCTTGAC AAACGTGGGG 
TTCTTTGCCA GAGTTAAGAT GCGGGAGCTG CTCCTCCTGT CGCGCGACCG GCGGATTCGG 
CGGGTGCTGA GACGGGAGCT GGTGAGGATG GCGGTCCTGA CCGAGGCGGA CTCCAGTCTT 
CTCGACAAAG TGGCGGCCAT CGAAGAGTGT TTTCCCCAGG AAGATGTGGG ACAGGCATCA 
TGGTTGGCCT CGCAGAAGCG AGCGCAGCTT AGGTTGGTCA CTCCACAGGC TTCTGAGCTT 
GCTGTGGCGG TACTGAAACC GGCGGAGTTG CCACCAAGGC AGGCACCGAA GGCAGGTGGC 
GACACTTGGC TCCTGGACGA GTCGCCCTAT TTCCGAGTGG CGCCTGCCTG GCTTCATTAC 
TGGGACGCCG ACACCTTAGA CAGAGTTTTG GCCGAGGGGC GTGCCGGCTC GCCTCTCCTG 
GATATGCAAG AGCTTGGAGG CGACAATTGG CGTCTACTTC TGGAGCACGG CCATCTGGAG 
CGTGCCCTGT CGGGTCCACT TCCCAGCGAA GCTCTTGAGG CGTCCTCCTG CATGTCCCAC 
GTGCATCGTC TGCTGTTGGC TGCACTGGCA AGGGGTGGCC AGATACATGA GGACGTTCTG 
GAGGACTTTG GGGACCTCCT GGGTCTCATG GGGGAGTCTG GCCTGAAGCC CTCGCAGCTG 
GCCAGGGTCT GCCACGGCCC CGTGACCCCC GAGGACTTTG TCCATCGCAT GGTGCACTAC 
TGCCTCCGGG AGAGGCTGCA CCACTTCCTG TACCTGTTTC TCACGGACCT CAGGCCAGAG 
CTTCCTCCAC TGTGCCCCCA GTGCAACGGC TGCTTTGTCC AGGCACTTCG GGATCATTGG 
CAGTGGCTGG ACGACCCACA AGATGTTGCA AAGGCAGAGC GTGCCATTGC ATCCTTGGCT 
CGAAGCCTGC CAGCAAAGGG CAGCGCAGTG CCGAGGATAT TGGAGCCGGC TGTTGTGGGT 
GCCGACCTCC AGGACATCTC TCTGTACCAG CTGCTGCAGA AAGGAACCCC CTTTGAGATA 
GCGGGCCTGT TTGCCTGGCA GACTACCAAC GCCTGTCAGG TGGACTCTGG CGGGTCGCTG 
ATGCCCCACT TCTCCCTGCC GTCCCTGCGC GACAAGTTTG GGCTGCGGGA GGAGCTGAGC 
TTCCCCTACT ACCTCCACAA GGGGCGCCCC TGTGCGGCTG CCTTCAAGTT CCTCGCGCAG 
GAGCTCCTGC TGCACGGATC TCTCTCCACC AAAAGGATAC GCTCAGCCTG TCATCGAGCT 
TTGCGGTTGG CGGAACAACG CCCCCACGAC GCCCGTGCCG TTGCTGCGAG CGTCGCTTTT 
GCGGAGATTC TGGGCATGGA TACTCGCAGC TTCCGAGTCA CCCTCCGGAC AACTCGGCTG 
GCCTTGACAT CTTCTCCTGG CTGCCAAAGT CGACTCAGCG ACATCCTGAG AGTGCTTCGT 
CGAAGCAAGG ACGCCATGCA GGAAGTCCTG CTGCAGGCAA CTCTTGCCTT AGAAGAACAG 
CTGACGGGTG CTGCGTCCTT CTCACCGATG GACGAGAAGT GCAGCAGCGG AATCTTGCTG 
CTTTTACGGT TTGCAACTGA GCACGGGTTT GAGGTGCCCG ACGTCGCGCT GCGATTGTGC 
GCAGCTGCGA ACGACTGGCT CGGTTTTCTG CTCTGGGCAC AGCTCCTTCA GTGCCCCAGG 
ACACAGGTGC TGGATCTAGT GCAGCTCTTC CAAGATGGCT GCCTCCGTGA GCACTTGAGC 
AAGGCGCTTT CACTAGTGAG CTCCCTGCAG CCGAGCAAGC ATCACCGCCG GGACCTCCGG 
GCATCTCTTT ATGTGCGCAT TGGACTTCAG GATCCCTCCA CCAGGGGGCA CGACCCTTCA 
GAATCGGCGG CTGAACAAGA TGCTGTGTCA GTGTGCAGCA GTGAAGGCAT CGACGCTGCT 
GCTGCCCTTA ACACCGAACC GCACTACTCG ACGGACCTCG TTGAGCTGAT GCTGCAGTGC 
CATTGCGGCG GCGGGGCTAC CCCCGGGTCT TCGGGCATGG CCTGGCAGAG CCTGCTGCGT 
GCTGCGGTGG TCCTGAAGCA CCAGACGCCC TGCGTCATTG CCGGCTGCTG TCCCGATGCC 
CCCCTGGGCA GCTGCCTGGC ATTGTGGCTC AAACTTTGTT CCGGCGGAGA GGAAGCGGAA 
ATGCCCGAGG ACGGTCGCCT TGAATTCACC CTGGCGGAGC TGCAGCAGAC GATGGAGGTG 
GTGGTCAAGA GGGGCCGACT GCGGACTCTT CTCCTGGGAC TCCAGCTCTT CCAGCCTAGA 
AGCCCTCTAG TCACACTCGT CGAGTTCCTG GTCGCATTCT TTCTGGAGCG GAACCGAAAA 
CGAGCCGAGG AATGCCTTGA GCGCCTCAAG CGCAAGTTCA CCAAGATGGA AGCCGCTTCA 
ACACCAGGGG TCGGCCTGAC CTCTCTGCCT TGGCTACGTG AGACCTCCCA GCGTCTGTTG 
GGACGCGCTT TTGGCCTGTG CCGTTCCCAC CTCGACCTGG CAGCCCTCGC GGCCTTCTGC 
GACAAGCTTC ACGGGGGCGA TAAGATGCCG CACCTCCACA AACTCCTCGC ATCTTTGAGT 
CCGGCGTCCC TGCTTCATGA GTTTGACTGG AGCTCGTTGT GGGGCCAACC GGGTGAAAGG 
GCGGCCACCC TGGCACGACT GGTGTCGCAG CTCAACCAGA CGGGCCGTTT CTCGGAGGCG 
CTGGCGCTGG CTAGGGCGGC GGGACTGCCA CTAGCGGACG TGGCGCTCCA GCAGCTGGAA 
CTGAGGTTCG AGGATCGCTC TGCCACCGCT AATGACACAT TCTGGGAGGA TTGCGACCAA 
GTGCTTCAGG AGAACTCCGT GGATCCTGCA CTCGCCTTCG ACTTTCTCAA GGGCAAAACG 
CTGCAAGTGG AAGGTGCACG GGAACTGTAC GAGGTGGGCC ACCGTGCGCT GCAGTGGCTG 
CGCCGCAGCG CTGCCTGGGG CTCGCCCGAG GCCCAGCGCT GGGAGGCGCG GGTGTGGCTA 
TGGTGTGTGC GCAGCGGGGC AGAGCCCACC CTCGAAGCGA ACCCCAAGCC AGACCCCCTG 
GTGGCACAGC CCCCTCCCAG CCCGGGGGCC TGCGTCACCC TGGACGACTC GCAGCAAGTG 
GCGGCGACGG AAAAGTTAAT TGACGAGCTG CTGTCTCGGG GCCACTTCTG CAAGGCTTGC 
CAGCTGGCTT TCCTGTTCGG CTGCTCCACA AGCGACCTGG AACTCTTGCT GACATGCATC 
CGTCTAGCAC AAGGAACGCA GTCGTCCGAC AACCTTGATC CACGAATCCA ACTTCGGATC 
AGCGAACCAG CACCAGTTCT GGCCCACAGG CTTTCAGTGG TTGCATCTAT GTCTGCTGTG 
GACTTGAAGT CCTTGGAAGG TGGCGAGGAC TCACCGGAGA GAAAGACACT CCAACTCCTG 
GAGCGCATCA CCAGAGCAGC GAAGAAGAGC CAGCAGCTGT GTCAGCGTGT GCTGCTCACC 
TTCACCCTTT CACTGAACCT GGGGTGCAGC TACTCGGTCC TGGCCCTGGA GTCGGACCCC 
GTGGCCCTTT TGGGCAACCT GGTGGGCCAT AGCCTGGCCA AGATGGAGGT CCAGAAGCGG 
GCGTCTGGCC AGAGGGACGG CGCCCGCCAG TGCTGCTCTG CGGACTTTGC CCTGGCCAAG 
AAACTGGTGG CCGTCTTCGG GATCCCGGAC GACCGGGTGG CAAACTTTCT CTTCCACATG 
GCCATGGACG CTATCCGAGG AAACGCAGTT GCAAGTAAAG CGGGCATCTT GGAGGTGTGG 
GACTTGGCCT TGGAGCTCTG CCCCGACCCT TCGCTACTGG GGAACCTTTT GCTCAGGGCT 
AGGGTTCACG ACCTGCGCGC TCTGAGCAGC AACCCCAAAG CGCTGTCTGT GGAGGTGGAG 
CTGTGCGTGC GCGCCCACAG CTGCTTCCTG GAGGCGTGCA GCATGGAGGG CATCTCGCGG 
GTGCTCCACC GCTGCCACCG TCTCACGCCC TGCTTGGTGG CGGGGCGGCA CTTCTCGCTG 
CTCGTGTCTT TGCTCACTGG CATGGCGCGC TACAGCGAGA TGGCCTACGT CTTTGACCTG 
CTCCTGCAGA ACCACCACTT TGAGCTGCTC TTCCAGCGGG GCATGGACAA GGTGCCTTAC 
CTGCGGGTGG CGCTTCTAGA CTACCTGAAA CACCGCGCCA GCACCGACCC AGATCTCTAC 
TCGATGCTCA CCCTCAACTT CAACATGCAT CGCGAGATTG CCGAGAGCCT CGAACTGACT 
GCACTGACCA AGATGAAGAA GCTCGTCACG GACGGACCAA TGGCATGGAG TCCCCAAGAG 
CAGCGGGCGC TGGAAACGGT GCTTCAAGAC CTGGCAGACG CAGCCGAGAG CTACGTCAAG 
GCGGAGTGCT TGCTGCGGGC GCAGTCGTGC GGCAGGAAGG CACAGCTGGT TGCCCTGCAG 
CTACGCTACT TCGCGTCACA GCTGGTGCTC ATCAACCTGG AGCCGTCAGC CGCCATGACG 
CAGGTGGCAC GTCACCCAAA CTTCTTTGAG GCACACATAG TGGCGGAGGC GTACGGCCTG 
CAGGGCTGGC ACAGCGCGGC CCTCTTCAGC AGGGTGCTCC TGGACGGCGA CTGGGGCTAC 
CTGGCCGACT TCTGCTCCGT CTGCGAGCTC ACCTCTCAGC ACGCACACGA GCTTGCACTC 
AGGTACCAGA ACGAAGCGGC CGGAAACGCA AAATGTCGGG ATGCGCTCGA GAAGCTCCTG 
GAACGTCTCC CGTGCGTGCT GTCTCGTCTG CAGCTGGCCC AGAAACTAGG CTTTGCACGC 
CTTGCGTCGC AGACTCTCGA GGCACACCCT TACCTTCGAG ACTACCTCGA CCAGCGGACG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8037553 ( XM_029973181.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029829041.1 Ixodes scapularis spatacsin (LOC8037553), transcript variant X3, mRNA.

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CloneID	OIa42604
Clone ID Related Accession (Same CDS sequence)	XM_029973180.1 , XM_029973181.1
Accession Version	XM_029973181.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	spatacsin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021924022.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGGT GCGTGCACCA AGGGAACATT CCGGAGGGCC AGTACCACCT GCTGCGTCGC 
AGGGCGGGCT CCGTCGAGCC CCTGCTGAGC ACCTTTCAGG AGGTTGCTTT GAGGGAGGCA 
TTGTGCCGGC TCCGTGAGGA TGACTTTCCC GAGGCGGAAT GGCTCTTGAC AAACGTGGGG 
TTCTTTGCCA GAGTTAAGAT GCGGGAGCTG CTCCTCCTGT CGCGCGACCG GCGGATTCGG 
CGGGTGCTGA GACGGGAGCT GGTGAGGATG GCGGTCCTGA CCGAGGCGGA CTCCAGTCTT 
CTCGACAAAG TGGCGGCCAT CGAAGAGTGT TTTCCCCAGG AAGATGTGGG ACAGGCATCA 
TGGTTGGCCT CGCAGAAGCG AGCGCAGCTT AGGTTGGTCA CTCCACAGGC TTCTGAGCTT 
GCTGTGGCGG TACTGAAACC GGCGGAGTTG CCACCAAGGC AGGCACCGAA GGCAGGTGGC 
GACACTTGGC TCCTGGACGA GTCGCCCTAT TTCCGAGTGG CGCCTGCCTG GCTTCATTAC 
TGGGACGCCG ACACCTTAGA CAGAGTTTTG GCCGAGGGGC GTGCCGGCTC GCCTCTCCTG 
GATATGCAAG AGCTTGGAGG CGACAATTGG CGTCTACTTC TGGAGCACGG CCATCTGGAG 
CGTGCCCTGT CGGGTCCACT TCCCAGCGAA GCTCTTGAGG CGTCCTCCTG CATGTCCCAC 
GTGCATCGTC TGCTGTTGGC TGCACTGGCA AGGGGTGGCC AGATACATGA GGACGTTCTG 
GAGGACTTTG GGGACCTCCT GGGTCTCATG GGGGAGTCTG GCCTGAAGCC CTCGCAGCTG 
GCCAGGGTCT GCCACGGCCC CGTGACCCCC GAGGACTTTG TCCATCGCAT GGTGCACTAC 
TGCCTCCGGG AGAGGCTGCA CCACTTCCTG TACCTGTTTC TCACGGACCT CAGGCCAGAG 
CTTCCTCCAC TGTGCCCCCA GTGCAACGGC TGCTTTGTCC AGGCACTTCG GGATCATTGG 
CAGTGGCTGG ACGACCCACA AGATGTTGCA AAGGCAGAGC GTGCCATTGC ATCCTTGGCT 
CGAAGCCTGC CAGCAAAGGG CAGCGCAGTG CCGAGGATAT TGGAGCCGGC TGTTGTGGGT 
GCCGACCTCC AGGACATCTC TCTGTACCAG CTGCTGCAGA AAGGAACCCC CTTTGAGATA 
GCGGGCCTGT TTGCCTGGCA GACTACCAAC GCCTGTCAGG TGGACTCTGG CGGGTCGCTG 
ATGCCCCACT TCTCCCTGCC GTCCCTGCGC GACAAGTTTG GGCTGCGGGA GGAGCTGAGC 
TTCCCCTACT ACCTCCACAA GGGGCGCCCC TGTGCGGCTG CCTTCAAGTT CCTCGCGCAG 
GAGCTCCTGC TGCACGGATC TCTCTCCACC AAAAGGATAC GCTCAGCCTG TCATCGAGCT 
TTGCGGTTGG CGGAACAACG CCCCCACGAC GCCCGTGCCG TTGCTGCGAG CGTCGCTTTT 
GCGGAGATTC TGGGCATGGA TACTCGCAGC TTCCGAGTCA CCCTCCGGAC AACTCGGCTG 
GCCTTGACAT CTTCTCCTGG CTGCCAAAGT CGACTCAGCG ACATCCTGAG AGTGCTTCGT 
CGAAGCAAGG ACGCCATGCA GGAAGTCCTG CTGCAGGCAA CTCTTGCCTT AGAAGAACAG 
CTGACGGGTG CTGCGTCCTT CTCACCGATG GACGAGAAGT GCAGCAGCGG AATCTTGCTG 
CTTTTACGGT TTGCAACTGA GCACGGGTTT GAGGTGCCCG ACGTCGCGCT GCGATTGTGC 
GCAGCTGCGA ACGACTGGCT CGGTTTTCTG CTCTGGGCAC AGCTCCTTCA GTGCCCCAGG 
ACACAGGTGC TGGATCTAGT GCAGCTCTTC CAAGATGGCT GCCTCCGTGA GCACTTGAGC 
AAGGCGCTTT CACTAGTGAG CTCCCTGCAG CCGAGCAAGC ATCACCGCCG GGACCTCCGG 
GCATCTCTTT ATGTGCGCAT TGGACTTCAG GATCCCTCCA CCAGGGGGCA CGACCCTTCA 
GAATCGGCGG CTGAACAAGA TGCTGTGTCA GTGTGCAGCA GTGAAGGCAT CGACGCTGCT 
GCTGCCCTTA ACACCGAACC GCACTACTCG ACGGACCTCG TTGAGCTGAT GCTGCAGTGC 
CATTGCGGCG GCGGGGCTAC CCCCGGGTCT TCGGGCATGG CCTGGCAGAG CCTGCTGCGT 
GCTGCGGTGG TCCTGAAGCA CCAGACGCCC TGCGTCATTG CCGGCTGCTG TCCCGATGCC 
CCCCTGGGCA GCTGCCTGGC ATTGTGGCTC AAACTTTGTT CCGGCGGAGA GGAAGCGGAA 
ATGCCCGAGG ACGGTCGCCT TGAATTCACC CTGGCGGAGC TGCAGCAGAC GATGGAGGTG 
GTGGTCAAGA GGGGCCGACT GCGGACTCTT CTCCTGGGAC TCCAGCTCTT CCAGCCTAGA 
AGCCCTCTAG TCACACTCGT CGAGTTCCTG GTCGCATTCT TTCTGGAGCG GAACCGAAAA 
CGAGCCGAGG AATGCCTTGA GCGCCTCAAG CGCAAGTTCA CCAAGATGGA AGCCGCTTCA 
ACACCAGGGG TCGGCCTGAC CTCTCTGCCT TGGCTACGTG AGACCTCCCA GCGTCTGTTG 
GGACGCGCTT TTGGCCTGTG CCGTTCCCAC CTCGACCTGG CAGCCCTCGC GGCCTTCTGC 
GACAAGCTTC ACGGGGGCGA TAAGATGCCG CACCTCCACA AACTCCTCGC ATCTTTGAGT 
CCGGCGTCCC TGCTTCATGA GTTTGACTGG AGCTCGTTGT GGGGCCAACC GGGTGAAAGG 
GCGGCCACCC TGGCACGACT GGTGTCGCAG CTCAACCAGA CGGGCCGTTT CTCGGAGGCG 
CTGGCGCTGG CTAGGGCGGC GGGACTGCCA CTAGCGGACG TGGCGCTCCA GCAGCTGGAA 
CTGAGGTTCG AGGATCGCTC TGCCACCGCT AATGACACAT TCTGGGAGGA TTGCGACCAA 
GTGCTTCAGG AGAACTCCGT GGATCCTGCA CTCGCCTTCG ACTTTCTCAA GGGCAAAACG 
CTGCAAGTGG AAGGTGCACG GGAACTGTAC GAGGTGGGCC ACCGTGCGCT GCAGTGGCTG 
CGCCGCAGCG CTGCCTGGGG CTCGCCCGAG GCCCAGCGCT GGGAGGCGCG GGTGTGGCTA 
TGGTGTGTGC GCAGCGGGGC AGAGCCCACC CTCGAAGCGA ACCCCAAGCC AGACCCCCTG 
GTGGCACAGC CCCCTCCCAG CCCGGGGGCC TGCGTCACCC TGGACGACTC GCAGCAAGTG 
GCGGCGACGG AAAAGTTAAT TGACGAGCTG CTGTCTCGGG GCCACTTCTG CAAGGCTTGC 
CAGCTGGCTT TCCTGTTCGG CTGCTCCACA AGCGACCTGG AACTCTTGCT GACATGCATC 
CGTCTAGCAC AAGGAACGCA GTCGTCCGAC AACCTTGATC CACGAATCCA ACTTCGGATC 
AGCGAACCAG CACCAGTTCT GGCCCACAGG CTTTCAGTGG TTGCATCTAT GTCTGCTGTG 
GACTTGAAGT CCTTGGAAGG TGGCGAGGAC TCACCGGAGA GAAAGACACT CCAACTCCTG 
GAGCGCATCA CCAGAGCAGC GAAGAAGAGC CAGCAGCTGT GTCAGCGTGT GCTGCTCACC 
TTCACCCTTT CACTGAACCT GGGGTGCAGC TACTCGGTCC TGGCCCTGGA GTCGGACCCC 
GTGGCCCTTT TGGGCAACCT GGTGGGCCAT AGCCTGGCCA AGATGGAGGT CCAGAAGCGG 
GCGTCTGGCC AGAGGGACGG CGCCCGCCAG TGCTGCTCTG CGGACTTTGC CCTGGCCAAG 
AAACTGGTGG CCGTCTTCGG GATCCCGGAC GACCGGGTGG CAAACTTTCT CTTCCACATG 
GCCATGGACG CTATCCGAGG AAACGCAGTT GCAAGTAAAG CGGGCATCTT GGAGGTGTGG 
GACTTGGCCT TGGAGCTCTG CCCCGACCCT TCGCTACTGG GGAACCTTTT GCTCAGGGCT 
AGGGTTCACG ACCTGCGCGC TCTGAGCAGC AACCCCAAAG CGCTGTCTGT GGAGGTGGAG 
CTGTGCGTGC GCGCCCACAG CTGCTTCCTG GAGGCGTGCA GCATGGAGGG CATCTCGCGG 
GTGCTCCACC GCTGCCACCG TCTCACGCCC TGCTTGGTGG CGGGGCGGCA CTTCTCGCTG 
CTCGTGTCTT TGCTCACTGG CATGGCGCGC TACAGCGAGA TGGCCTACGT CTTTGACCTG 
CTCCTGCAGA ACCACCACTT TGAGCTGCTC TTCCAGCGGG GCATGGACAA GGTGCCTTAC 
CTGCGGGTGG CGCTTCTAGA CTACCTGAAA CACCGCGCCA GCACCGACCC AGATCTCTAC 
TCGATGCTCA CCCTCAACTT CAACATGCAT CGCGAGATTG CCGAGAGCCT CGAACTGACT 
GCACTGACCA AGATGAAGAA GCTCGTCACG GACGGACCAA TGGCATGGAG TCCCCAAGAG 
CAGCGGGCGC TGGAAACGGT GCTTCAAGAC CTGGCAGACG CAGCCGAGAG CTACGTCAAG 
GCGGAGTGCT TGCTGCGGGC GCAGTCGTGC GGCAGGAAGG CACAGCTGGT TGCCCTGCAG 
CTACGCTACT TCGCGTCACA GCTGGTGCTC ATCAACCTGG AGCCGTCAGC CGCCATGACG 
CAGGTGGCAC GTCACCCAAA CTTCTTTGAG GCACACATAG TGGCGGAGGC GTACGGCCTG 
CAGGGCTGGC ACAGCGCGGC CCTCTTCAGC AGGGTGCTCC TGGACGGCGA CTGGGGCTAC 
CTGGCCGACT TCTGCTCCGT CTGCGAGCTC ACCTCTCAGC ACGCACACGA GCTTGCACTC 
AGGTACCAGA ACGAAGCGGC CGGAAACGCA AAATGTCGGG ATGCGCTCGA GAAGCTCCTG 
GAACGTCTCC CGTGCGTGCT GTCTCGTCTG CAGCTGGCCC AGAAACTAGG CTTTGCACGC 
CTTGCGTCGC AGACTCTCGA GGCACACCCT TACCTTCGAG ACTACCTCGA CCAGCGGACG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029829041.1
CDS	26..5068
Translation	MLRCVHQGNIPEGQYHLLRRRAGSVEPLLSTFQEVALREALCRLREDDFPEAEWLLTNVGFFARVKMRELLLLSRDRRIRRVLRRELVRMAVLTEADSSLLDKVAAIEECFPQEDVGQASWLASQKRAQLRLVTPQASELAVAVLKPAELPPRQAPKAGGDTWLLDESPYFRVAPAWLHYWDADTLDRVLAEGRAGSPLLDMQELGGDNWRLLLEHGHLERALSGPLPSEALEASSCMSHVHRLLLAALARGGQIHEDVLEDFGDLLGLMGESGLKPSQLARVCHGPVTPEDFVHRMVHYCLRERLHHFLYLFLTDLRPELPPLCPQCNGCFVQALRDHWQWLDDPQDVAKAERAIASLARSLPAKGSAVPRILEPAVVGADLQDISLYQLLQKGTPFEIAGLFAWQTTNACQVDSGGSLMPHFSLPSLRDKFGLREELSFPYYLHKGRPCAAAFKFLAQELLLHGSLSTKRIRSACHRALRLAEQRPHDARAVAASVAFAEILGMDTRSFRVTLRTTRLALTSSPGCQSRLSDILRVLRRSKDAMQEVLLQATLALEEQLTGAASFSPMDEKCSSGILLLLRFATEHGFEVPDVALRLCAAANDWLGFLLWAQLLQCPRTQVLDLVQLFQDGCLREHLSKALSLVSSLQPSKHHRRDLRASLYVRIGLQDPSTRGHDPSESAAEQDAVSVCSSEGIDAAAALNTEPHYSTDLVELMLQCHCGGGATPGSSGMAWQSLLRAAVVLKHQTPCVIAGCCPDAPLGSCLALWLKLCSGGEEAEMPEDGRLEFTLAELQQTMEVVVKRGRLRTLLLGLQLFQPRSPLVTLVEFLVAFFLERNRKRAEECLERLKRKFTKMEAASTPGVGLTSLPWLRETSQRLLGRAFGLCRSHLDLAALAAFCDKLHGGDKMPHLHKLLASLSPASLLHEFDWSSLWGQPGERAATLARLVSQLNQTGRFSEALALARAAGLPLADVALQQLELRFEDRSATANDTFWEDCDQVLQENSVDPALAFDFLKGKTLQVEGARELYEVGHRALQWLRRSAAWGSPEAQRWEARVWLWCVRSGAEPTLEANPKPDPLVAQPPPSPGACVTLDDSQQVAATEKLIDELLSRGHFCKACQLAFLFGCSTSDLELLLTCIRLAQGTQSSDNLDPRIQLRISEPAPVLAHRLSVVASMSAVDLKSLEGGEDSPERKTLQLLERITRAAKKSQQLCQRVLLTFTLSLNLGCSYSVLALESDPVALLGNLVGHSLAKMEVQKRASGQRDGARQCCSADFALAKKLVAVFGIPDDRVANFLFHMAMDAIRGNAVASKAGILEVWDLALELCPDPSLLGNLLLRARVHDLRALSSNPKALSVEVELCVRAHSCFLEACSMEGISRVLHRCHRLTPCLVAGRHFSLLVSLLTGMARYSEMAYVFDLLLQNHHFELLFQRGMDKVPYLRVALLDYLKHRASTDPDLYSMLTLNFNMHREIAESLELTALTKMKKLVTDGPMAWSPQEQRALETVLQDLADAAESYVKAECLLRAQSCGRKAQLVALQLRYFASQLVLINLEPSAAMTQVARHPNFFEAHIVAEAYGLQGWHSAALFSRVLLDGDWGYLADFCSVCELTSQHAHELALRYQNEAAGNAKCRDALEKLLERLPCVLSRLQLAQKLGFARLASQTLEAHPYLRDYLDQRT

Target ORF information:

RefSeq Version	XM_029973181.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis spatacsin (LOC8037553), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029973181.1

ORF Insert Sequence:

ATGCTGCGGT GCGTGCACCA AGGGAACATT CCGGAGGGCC AGTACCACCT GCTGCGTCGC 
AGGGCGGGCT CCGTCGAGCC CCTGCTGAGC ACCTTTCAGG AGGTTGCTTT GAGGGAGGCA 
TTGTGCCGGC TCCGTGAGGA TGACTTTCCC GAGGCGGAAT GGCTCTTGAC AAACGTGGGG 
TTCTTTGCCA GAGTTAAGAT GCGGGAGCTG CTCCTCCTGT CGCGCGACCG GCGGATTCGG 
CGGGTGCTGA GACGGGAGCT GGTGAGGATG GCGGTCCTGA CCGAGGCGGA CTCCAGTCTT 
CTCGACAAAG TGGCGGCCAT CGAAGAGTGT TTTCCCCAGG AAGATGTGGG ACAGGCATCA 
TGGTTGGCCT CGCAGAAGCG AGCGCAGCTT AGGTTGGTCA CTCCACAGGC TTCTGAGCTT 
GCTGTGGCGG TACTGAAACC GGCGGAGTTG CCACCAAGGC AGGCACCGAA GGCAGGTGGC 
GACACTTGGC TCCTGGACGA GTCGCCCTAT TTCCGAGTGG CGCCTGCCTG GCTTCATTAC 
TGGGACGCCG ACACCTTAGA CAGAGTTTTG GCCGAGGGGC GTGCCGGCTC GCCTCTCCTG 
GATATGCAAG AGCTTGGAGG CGACAATTGG CGTCTACTTC TGGAGCACGG CCATCTGGAG 
CGTGCCCTGT CGGGTCCACT TCCCAGCGAA GCTCTTGAGG CGTCCTCCTG CATGTCCCAC 
GTGCATCGTC TGCTGTTGGC TGCACTGGCA AGGGGTGGCC AGATACATGA GGACGTTCTG 
GAGGACTTTG GGGACCTCCT GGGTCTCATG GGGGAGTCTG GCCTGAAGCC CTCGCAGCTG 
GCCAGGGTCT GCCACGGCCC CGTGACCCCC GAGGACTTTG TCCATCGCAT GGTGCACTAC 
TGCCTCCGGG AGAGGCTGCA CCACTTCCTG TACCTGTTTC TCACGGACCT CAGGCCAGAG 
CTTCCTCCAC TGTGCCCCCA GTGCAACGGC TGCTTTGTCC AGGCACTTCG GGATCATTGG 
CAGTGGCTGG ACGACCCACA AGATGTTGCA AAGGCAGAGC GTGCCATTGC ATCCTTGGCT 
CGAAGCCTGC CAGCAAAGGG CAGCGCAGTG CCGAGGATAT TGGAGCCGGC TGTTGTGGGT 
GCCGACCTCC AGGACATCTC TCTGTACCAG CTGCTGCAGA AAGGAACCCC CTTTGAGATA 
GCGGGCCTGT TTGCCTGGCA GACTACCAAC GCCTGTCAGG TGGACTCTGG CGGGTCGCTG 
ATGCCCCACT TCTCCCTGCC GTCCCTGCGC GACAAGTTTG GGCTGCGGGA GGAGCTGAGC 
TTCCCCTACT ACCTCCACAA GGGGCGCCCC TGTGCGGCTG CCTTCAAGTT CCTCGCGCAG 
GAGCTCCTGC TGCACGGATC TCTCTCCACC AAAAGGATAC GCTCAGCCTG TCATCGAGCT 
TTGCGGTTGG CGGAACAACG CCCCCACGAC GCCCGTGCCG TTGCTGCGAG CGTCGCTTTT 
GCGGAGATTC TGGGCATGGA TACTCGCAGC TTCCGAGTCA CCCTCCGGAC AACTCGGCTG 
GCCTTGACAT CTTCTCCTGG CTGCCAAAGT CGACTCAGCG ACATCCTGAG AGTGCTTCGT 
CGAAGCAAGG ACGCCATGCA GGAAGTCCTG CTGCAGGCAA CTCTTGCCTT AGAAGAACAG 
CTGACGGGTG CTGCGTCCTT CTCACCGATG GACGAGAAGT GCAGCAGCGG AATCTTGCTG 
CTTTTACGGT TTGCAACTGA GCACGGGTTT GAGGTGCCCG ACGTCGCGCT GCGATTGTGC 
GCAGCTGCGA ACGACTGGCT CGGTTTTCTG CTCTGGGCAC AGCTCCTTCA GTGCCCCAGG 
ACACAGGTGC TGGATCTAGT GCAGCTCTTC CAAGATGGCT GCCTCCGTGA GCACTTGAGC 
AAGGCGCTTT CACTAGTGAG CTCCCTGCAG CCGAGCAAGC ATCACCGCCG GGACCTCCGG 
GCATCTCTTT ATGTGCGCAT TGGACTTCAG GATCCCTCCA CCAGGGGGCA CGACCCTTCA 
GAATCGGCGG CTGAACAAGA TGCTGTGTCA GTGTGCAGCA GTGAAGGCAT CGACGCTGCT 
GCTGCCCTTA ACACCGAACC GCACTACTCG ACGGACCTCG TTGAGCTGAT GCTGCAGTGC 
CATTGCGGCG GCGGGGCTAC CCCCGGGTCT TCGGGCATGG CCTGGCAGAG CCTGCTGCGT 
GCTGCGGTGG TCCTGAAGCA CCAGACGCCC TGCGTCATTG CCGGCTGCTG TCCCGATGCC 
CCCCTGGGCA GCTGCCTGGC ATTGTGGCTC AAACTTTGTT CCGGCGGAGA GGAAGCGGAA 
ATGCCCGAGG ACGGTCGCCT TGAATTCACC CTGGCGGAGC TGCAGCAGAC GATGGAGGTG 
GTGGTCAAGA GGGGCCGACT GCGGACTCTT CTCCTGGGAC TCCAGCTCTT CCAGCCTAGA 
AGCCCTCTAG TCACACTCGT CGAGTTCCTG GTCGCATTCT TTCTGGAGCG GAACCGAAAA 
CGAGCCGAGG AATGCCTTGA GCGCCTCAAG CGCAAGTTCA CCAAGATGGA AGCCGCTTCA 
ACACCAGGGG TCGGCCTGAC CTCTCTGCCT TGGCTACGTG AGACCTCCCA GCGTCTGTTG 
GGACGCGCTT TTGGCCTGTG CCGTTCCCAC CTCGACCTGG CAGCCCTCGC GGCCTTCTGC 
GACAAGCTTC ACGGGGGCGA TAAGATGCCG CACCTCCACA AACTCCTCGC ATCTTTGAGT 
CCGGCGTCCC TGCTTCATGA GTTTGACTGG AGCTCGTTGT GGGGCCAACC GGGTGAAAGG 
GCGGCCACCC TGGCACGACT GGTGTCGCAG CTCAACCAGA CGGGCCGTTT CTCGGAGGCG 
CTGGCGCTGG CTAGGGCGGC GGGACTGCCA CTAGCGGACG TGGCGCTCCA GCAGCTGGAA 
CTGAGGTTCG AGGATCGCTC TGCCACCGCT AATGACACAT TCTGGGAGGA TTGCGACCAA 
GTGCTTCAGG AGAACTCCGT GGATCCTGCA CTCGCCTTCG ACTTTCTCAA GGGCAAAACG 
CTGCAAGTGG AAGGTGCACG GGAACTGTAC GAGGTGGGCC ACCGTGCGCT GCAGTGGCTG 
CGCCGCAGCG CTGCCTGGGG CTCGCCCGAG GCCCAGCGCT GGGAGGCGCG GGTGTGGCTA 
TGGTGTGTGC GCAGCGGGGC AGAGCCCACC CTCGAAGCGA ACCCCAAGCC AGACCCCCTG 
GTGGCACAGC CCCCTCCCAG CCCGGGGGCC TGCGTCACCC TGGACGACTC GCAGCAAGTG 
GCGGCGACGG AAAAGTTAAT TGACGAGCTG CTGTCTCGGG GCCACTTCTG CAAGGCTTGC 
CAGCTGGCTT TCCTGTTCGG CTGCTCCACA AGCGACCTGG AACTCTTGCT GACATGCATC 
CGTCTAGCAC AAGGAACGCA GTCGTCCGAC AACCTTGATC CACGAATCCA ACTTCGGATC 
AGCGAACCAG CACCAGTTCT GGCCCACAGG CTTTCAGTGG TTGCATCTAT GTCTGCTGTG 
GACTTGAAGT CCTTGGAAGG TGGCGAGGAC TCACCGGAGA GAAAGACACT CCAACTCCTG 
GAGCGCATCA CCAGAGCAGC GAAGAAGAGC CAGCAGCTGT GTCAGCGTGT GCTGCTCACC 
TTCACCCTTT CACTGAACCT GGGGTGCAGC TACTCGGTCC TGGCCCTGGA GTCGGACCCC 
GTGGCCCTTT TGGGCAACCT GGTGGGCCAT AGCCTGGCCA AGATGGAGGT CCAGAAGCGG 
GCGTCTGGCC AGAGGGACGG CGCCCGCCAG TGCTGCTCTG CGGACTTTGC CCTGGCCAAG 
AAACTGGTGG CCGTCTTCGG GATCCCGGAC GACCGGGTGG CAAACTTTCT CTTCCACATG 
GCCATGGACG CTATCCGAGG AAACGCAGTT GCAAGTAAAG CGGGCATCTT GGAGGTGTGG 
GACTTGGCCT TGGAGCTCTG CCCCGACCCT TCGCTACTGG GGAACCTTTT GCTCAGGGCT 
AGGGTTCACG ACCTGCGCGC TCTGAGCAGC AACCCCAAAG CGCTGTCTGT GGAGGTGGAG 
CTGTGCGTGC GCGCCCACAG CTGCTTCCTG GAGGCGTGCA GCATGGAGGG CATCTCGCGG 
GTGCTCCACC GCTGCCACCG TCTCACGCCC TGCTTGGTGG CGGGGCGGCA CTTCTCGCTG 
CTCGTGTCTT TGCTCACTGG CATGGCGCGC TACAGCGAGA TGGCCTACGT CTTTGACCTG 
CTCCTGCAGA ACCACCACTT TGAGCTGCTC TTCCAGCGGG GCATGGACAA GGTGCCTTAC 
CTGCGGGTGG CGCTTCTAGA CTACCTGAAA CACCGCGCCA GCACCGACCC AGATCTCTAC 
TCGATGCTCA CCCTCAACTT CAACATGCAT CGCGAGATTG CCGAGAGCCT CGAACTGACT 
GCACTGACCA AGATGAAGAA GCTCGTCACG GACGGACCAA TGGCATGGAG TCCCCAAGAG 
CAGCGGGCGC TGGAAACGGT GCTTCAAGAC CTGGCAGACG CAGCCGAGAG CTACGTCAAG 
GCGGAGTGCT TGCTGCGGGC GCAGTCGTGC GGCAGGAAGG CACAGCTGGT TGCCCTGCAG 
CTACGCTACT TCGCGTCACA GCTGGTGCTC ATCAACCTGG AGCCGTCAGC CGCCATGACG 
CAGGTGGCAC GTCACCCAAA CTTCTTTGAG GCACACATAG TGGCGGAGGC GTACGGCCTG 
CAGGGCTGGC ACAGCGCGGC CCTCTTCAGC AGGGTGCTCC TGGACGGCGA CTGGGGCTAC 
CTGGCCGACT TCTGCTCCGT CTGCGAGCTC ACCTCTCAGC ACGCACACGA GCTTGCACTC 
AGGTACCAGA ACGAAGCGGC CGGAAACGCA AAATGTCGGG ATGCGCTCGA GAAGCTCCTG 
GAACGTCTCC CGTGCGTGCT GTCTCGTCTG CAGCTGGCCC AGAAACTAGG CTTTGCACGC 
CTTGCGTCGC AGACTCTCGA GGCACACCCT TACCTTCGAG ACTACCTCGA CCAGCGGACG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8037553 ( XM_029973179.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029829039.1 Ixodes scapularis spatacsin (LOC8037553), transcript variant X1, mRNA.

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CloneID	OIa42603
Clone ID Related Accession (Same CDS sequence)	XM_029973179.1
Accession Version	XM_029973179.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	spatacsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021924022.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCTAA CTCTGCTCAA GAAACTCAAA GGCCCTCGGT CCGAGCCACG CGCACACATC 
GTCAGTCCGT CCCCGCTGCT AGTGTCGTAC TGTTTCATCC ACGAGTCGAA CCTGAAAGTC 
GTCGACCTGA AAACGGACCG TGTCATTTCG TCCTTACGCG ACGTTCAGGA CTGTATCTGG 
AGGGACGGTC TCGCCGACGC CGACGAACTT GCGGTCGTCT CGAGCGGACA TGAAGTTTCC 
CTGGTGCGAT ACGACGGGCA CGGCTGTACT TACAAGCAGA GATCTCTGCG TTGGAACTCC 
GTCCAAAGAC TGGCGAAGGT CGAATGGGTG CTCTGCCACG GTGACAACTT CGTCTTCCTG 
ATCAACCGGT CTCTCTTGCT GTGCACTCTT TTGATCGGAG ATGCTCTCGA GACGGTGTGG 
CACTCAGACT TGGGTTGCGA GGTGGTGCGG GTGAGGGTGA TAGACTCCGA GGTGTACGTG 
CTTACGTCGC AAGCAAGCAT TGTAGGGTAC AGCCTCCAAA CTGGGTTGCG ATCCTCTGTT 
CTGGAGCTAC AGTCCTGCCC CTTCGATGGC AGCCAGGTCA CAGACTTCGT CCTGTGCCCC 
GAACAAGGTG CCCTCGCAGT ATGCGTCGGG CACGCAAGGC TTTACTCAGT CGTGGCGGAG 
AAGCCCGCCT TCGGCGAGAG CTGGCGCCTC GCGCCGACCC CTAAACATGG GGTGAGGGTC 
ACGGCGTTTG TGAAGAAAAA AACGCTCGAC AATGAAGTGT GTGCCACCCG GAATTGGAAG 
CTGCTTTTGG ACGTGTCCGA CGAAGCGGTG AACAGTCACG TCATGTGGAT GGGCGAAGAC 
TGGAGGATTG TGTTTTGCTA CGGCGCAGGA GGACTGCAGA GGGTCGTGGC TTTCGAGATG 
GGTACAGATG GCGCGTTCTC GCCCATTGTC GAGCAGGCGG ATCTTTCGAG CCACGGGAGC 
TTTGTGTTAA TGCACGACAC GAGGAGTACC CTGCAGTGCG CCCCGGCATT CCTGTTTGCC 
GACAACACCG CCACCCTCAC CTTTGGCGCC GCCAGCCGTC GACTGTTGGC TGAGGTCATG 
GAGCAGACTG GGCGACGTGC TGCGGAGACG CTGTCGCAGC TCAACCGGTG GCAGGCGATG 
GCCGTGGACT TGTCCGTTCT GCTCCGGGGA GTTCGACACC GGCAGCTCGA CATGCTGGCT 
TTTTTTCTCA AGGCCAAGGA GGACGCCATG GAGCTCCAGC TGACGAGAAG TCGGCCCAAG 
GACACCGGGG ATGCGCAGCA GCTCGAGTTG CTCGCCTCGG AAGTGAACGC CTGGGACAGC 
GTCCTGACCG AACTGGGCCA TGCAGTCGTG GAAGCCTTAG GCGATCTCTC GTCCAAACAC 
TTCTCACAGC AGCTGCTTCA GCTTGTCCTG TCGAGTGTTG TTCGTCTTCT GGCGATGGCA 
TTGCCTCGAG AGTTGAACGA CACTTGGCCC GAGAGAGTTC AGCTTCGAAA GCGGATGTCT 
GTCATGCTGG CAGGCCATCT GGGGAAGCTC CGAAGCCAGA TCCATCACGG ATCGGTTTTG 
GCAGCTGCCG TTCCGGAGCT GAAGGATGCA GCACAGGCTG ATGCTTCTGA ATGGGACAAG 
CTCACCGAGA AAGAACTCAT GCTGCGGTGC GTGCACCAAG GGAACATTCC GGAGGGCCAG 
TACCACCTGC TGCGTCGCAG GGCGGGCTCC GTCGAGCCCC TGCTGAGCAC CTTTCAGGAG 
GTTGCTTTGA GGGAGGCATT GTGCCGGCTC CGTGAGGATG ACTTTCCCGA GGCGGAATGG 
CTCTTGACAA ACGTGGGGTT CTTTGCCAGA GTTAAGATGC GGGAGCTGCT CCTCCTGTCG 
CGCGACCGGC GGATTCGGCG GGTGCTGAGA CGGGAGCTGG TGAGGATGGC GGTCCTGACC 
GAGGCGGACT CCAGTCTTCT CGACAAAGTG GCGGCCATCG AAGAGTGTTT TCCCCAGGAA 
GATGTGGGAC AGGCATCATG GTTGGCCTCG CAGAAGCGAG CGCAGCTTAG GTTGGTCACT 
CCACAGGCTT CTGAGCTTGC TGTGGCGGTA CTGAAACCGG CGGAGTTGCC ACCAAGGCAG 
GCACCGAAGG CAGGTGGCGA CACTTGGCTC CTGGACGAGT CGCCCTATTT CCGAGTGGCG 
CCTGCCTGGC TTCATTACTG GGACGCCGAC ACCTTAGACA GAGTTTTGGC CGAGGGGCGT 
GCCGGCTCGC CTCTCCTGGA TATGCAAGAG CTTGGAGGCG ACAATTGGCG TCTACTTCTG 
GAGCACGGCC ATCTGGAGCG TGCCCTGTCG GGTCCACTTC CCAGCGAAGC TCTTGAGGCG 
TCCTCCTGCA TGTCCCACGT GCATCGTCTG CTGTTGGCTG CACTGGCAAG GGGTGGCCAG 
ATACATGAGG ACGTTCTGGA GGACTTTGGG GACCTCCTGG GTCTCATGGG GGAGTCTGGC 
CTGAAGCCCT CGCAGCTGGC CAGGGTCTGC CACGGCCCCG TGACCCCCGA GGACTTTGTC 
CATCGCATGG TGCACTACTG CCTCCGGGAG AGGCTGCACC ACTTCCTGTA CCTGTTTCTC 
ACGGACCTCA GGCCAGAGCT TCCTCCACTG TGCCCCCAGT GCAACGGCTG CTTTGTCCAG 
GCACTTCGGG ATCATTGGCA GTGGCTGGAC GACCCACAAG ATGTTGCAAA GGCAGAGCGT 
GCCATTGCAT CCTTGGCTCG AAGCCTGCCA GCAAAGGGCA GCGCAGTGCC GAGGATATTG 
GAGCCGGCTG TTGTGGGTGC CGACCTCCAG GACATCTCTC TGTACCAGCT GCTGCAGAAA 
GGAACCCCCT TTGAGATAGC GGGCCTGTTT GCCTGGCAGA CTACCAACGC CTGTCAGGTG 
GACTCTGGCG GGTCGCTGAT GCCCCACTTC TCCCTGCCGT CCCTGCGCGA CAAGTTTGGG 
CTGCGGGAGG AGCTGAGCTT CCCCTACTAC CTCCACAAGG GGCGCCCCTG TGCGGCTGCC 
TTCAAGTTCC TCGCGCAGGA GCTCCTGCTG CACGGATCTC TCTCCACCAA AAGGATACGC 
TCAGCCTGTC ATCGAGCTTT GCGGTTGGCG GAACAACGCC CCCACGACGC CCGTGCCGTT 
GCTGCGAGCG TCGCTTTTGC GGAGATTCTG GGCATGGATA CTCGCAGCTT CCGAGTCACC 
CTCCGGACAA CTCGGCTGGC CTTGACATCT TCTCCTGGCT GCCAAAGTCG ACTCAGCGAC 
ATCCTGAGAG TGCTTCGTCG AAGCAAGGAC GCCATGCAGG AAGTCCTGCT GCAGGCAACT 
CTTGCCTTAG AAGAACAGCT GACGGGTGCT GCGTCCTTCT CACCGATGGA CGAGAAGTGC 
AGCAGCGGAA TCTTGCTGCT TTTACGGTTT GCAACTGAGC ACGGGTTTGA GGTGCCCGAC 
GTCGCGCTGC GATTGTGCGC AGCTGCGAAC GACTGGCTCG GTTTTCTGCT CTGGGCACAG 
CTCCTTCAGT GCCCCAGGAC ACAGGTGCTG GATCTAGTGC AGCTCTTCCA AGATGGCTGC 
CTCCGTGAGC ACTTGAGCAA GGCGCTTTCA CTAGTGAGCT CCCTGCAGCC GAGCAAGCAT 
CACCGCCGGG ACCTCCGGGC ATCTCTTTAT GTGCGCATTG GACTTCAGGA TCCCTCCACC 
AGGGGGCACG ACCCTTCAGA ATCGGCGGCT GAACAAGATG CTGTGTCAGT GTGCAGCAGT 
GAAGGCATCG ACGCTGCTGC TGCCCTTAAC ACCGAACCGC ACTACTCGAC GGACCTCGTT 
GAGCTGATGC TGCAGTGCCA TTGCGGCGGC GGGGCTACCC CCGGGTCTTC GGGCATGGCC 
TGGCAGAGCC TGCTGCGTGC TGCGGTGGTC CTGAAGCACC AGACGCCCTG CGTCATTGCC 
GGCTGCTGTC CCGATGCCCC CCTGGGCAGC TGCCTGGCAT TGTGGCTCAA ACTTTGTTCC 
GGCGGAGAGG AAGCGGAAAT GCCCGAGGAC GGTCGCCTTG AATTCACCCT GGCGGAGCTG 
CAGCAGACGA TGGAGGTGGT GGTCAAGAGG GGCCGACTGC GGACTCTTCT CCTGGGACTC 
CAGCTCTTCC AGCCTAGAAG CCCTCTAGTC ACACTCGTCG AGTTCCTGGT CGCATTCTTT 
CTGGAGCGGA ACCGAAAACG AGCCGAGGAA TGCCTTGAGC GCCTCAAGCG CAAGTTCACC 
AAGATGGAAG CCGCTTCAAC ACCAGGGGTC GGCCTGACCT CTCTGCCTTG GCTACGTGAG 
ACCTCCCAGC GTCTGTTGGG ACGCGCTTTT GGCCTGTGCC GTTCCCACCT CGACCTGGCA 
GCCCTCGCGG CCTTCTGCGA CAAGCTTCAC GGGGGCGATA AGATGCCGCA CCTCCACAAA 
CTCCTCGCAT CTTTGAGTCC GGCGTCCCTG CTTCATGAGT TTGACTGGAG CTCGTTGTGG 
GGCCAACCGG GTGAAAGGGC GGCCACCCTG GCACGACTGG TGTCGCAGCT CAACCAGACG 
GGCCGTTTCT CGGAGGCGCT GGCGCTGGCT AGGGCGGCGG GACTGCCACT AGCGGACGTG 
GCGCTCCAGC AGCTGGAACT GAGGTTCGAG GATCGCTCTG CCACCGCTAA TGACACATTC 
TGGGAGGATT GCGACCAAGT GCTTCAGGAG AACTCCGTGG ATCCTGCACT CGCCTTCGAC 
TTTCTCAAGG GCAAAACGCT GCAAGTGGAA GGTGCACGGG AACTGTACGA GGTGGGCCAC 
CGTGCGCTGC AGTGGCTGCG CCGCAGCGCT GCCTGGGGCT CGCCCGAGGC CCAGCGCTGG 
GAGGCGCGGG TGTGGCTATG GTGTGTGCGC AGCGGGGCAG AGCCCACCCT CGAAGCGAAC 
CCCAAGCCAG ACCCCCTGGT GGCACAGCCC CCTCCCAGCC CGGGGGCCTG CGTCACCCTG 
GACGACTCGC AGCAAGTGGC GGCGACGGAA AAGTTAATTG ACGAGCTGCT GTCTCGGGGC 
CACTTCTGCA AGGCTTGCCA GCTGGCTTTC CTGTTCGGCT GCTCCACAAG CGACCTGGAA 
CTCTTGCTGA CATGCATCCG TCTAGCACAA GGAACGCAGT CGTCCGACAA CCTTGATCCA 
CGAATCCAAC TTCGGATCAG CGAACCAGCA CCAGTTCTGG CCCACAGGCT TTCAGTGGTT 
GCATCTATGT CTGCTGTGGA CTTGAAGTCC TTGGAAGGTG GCGAGGACTC ACCGGAGAGA 
AAGACACTCC AACTCCTGGA GCGCATCACC AGAGCAGCGA AGAAGAGCCA GCAGCTGTGT 
CAGCGTGTGC TGCTCACCTT CACCCTTTCA CTGAACCTGG GGTGCAGCTA CTCGGTCCTG 
GCCCTGGAGT CGGACCCCGT GGCCCTTTTG GGCAACCTGG TGGGCCATAG CCTGGCCAAG 
ATGGAGGTCC AGAAGCGGGC GTCTGGCCAG AGGGACGGCG CCCGCCAGTG CTGCTCTGCG 
GACTTTGCCC TGGCCAAGAA ACTGGTGGCC GTCTTCGGGA TCCCGGACGA CCGGGTGGCA 
AACTTTCTCT TCCACATGGC CATGGACGCT ATCCGAGGAA ACGCAGTTGC AAGTAAAGCG 
GGCATCTTGG AGGTGTGGGA CTTGGCCTTG GAGCTCTGCC CCGACCCTTC GCTACTGGGG 
AACCTTTTGC TCAGGGCTAG GGTTCACGAC CTGCGCGCTC TGAGCAGCAA CCCCAAAGCG 
CTGTCTGTGG AGGTGGAGCT GTGCGTGCGC GCCCACAGCT GCTTCCTGGA GGCGTGCAGC 
ATGGAGGGCA TCTCGCGGGT GCTCCACCGC TGCCACCGTC TCACGCCCTG CTTGGTGGCG 
GGGCGGCACT TCTCGCTGCT CGTGTCTTTG CTCACTGGCA TGGCGCGCTA CAGCGAGATG 
GCCTACGTCT TTGACCTGCT CCTGCAGAAC CACCACTTTG AGCTGCTCTT CCAGCGGGGC 
ATGGACAAGG TGCCTTACCT GCGGGTGGCG CTTCTAGACT ACCTGAAACA CCGCGCCAGC 
ACCGACCCAG ATCTCTACTC GATGCTCACC CTCAACTTCA ACATGCATCG CGAGATTGCC 
GAGAGCCTCG AACTGACTGC ACTGACCAAG ATGAAGAAGC TCGTCACGGA CGGACCAATG 
GCATGGAGTC CCCAAGAGCA GCGGGCGCTG GAAACGGTGC TTCAAGACCT GGCAGACGCA 
GCCGAGAGCT ACGTCAAGGC GGAGTGCTTG CTGCGGGCGC AGTCGTGCGG CAGGAAGGCA 
CAGCTGGTTG CCCTGCAGCT ACGCTACTTC GCGTCACAGC TGGTGCTCAT CAACCTGGAG 
CCGTCAGCCG CCATGACGCA GGTGGCACGT CACCCAAACT TCTTTGAGGC ACACATAGTG 
GCGGAGGCGT ACGGCCTGCA GGGCTGGCAC AGCGCGGCCC TCTTCAGCAG GGTGCTCCTG 
GACGGCGACT GGGGCTACCT GGCCGACTTC TGCTCCGTCT GCGAGCTCAC CTCTCAGCAC 
GCACACGAGC TTGCACTCAG GTACCAGAAC GAAGCGGCCG GAAACGCAAA ATGTCGGGAT 
GCGCTCGAGA AGCTCCTGGA ACGTCTCCCG TGCGTGCTGT CTCGTCTGCA GCTGGCCCAG 
AAACTAGGCT TTGCACGCCT TGCGTCGCAG ACTCTCGAGG CACACCCTTA CCTTCGAGAC 
TACCTCGACC AGCGGACGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029829039.1
CDS	89..6769
Translation	MTLTLLKKLKGPRSEPRAHIVSPSPLLVSYCFIHESNLKVVDLKTDRVISSLRDVQDCIWRDGLADADELAVVSSGHEVSLVRYDGHGCTYKQRSLRWNSVQRLAKVEWVLCHGDNFVFLINRSLLLCTLLIGDALETVWHSDLGCEVVRVRVIDSEVYVLTSQASIVGYSLQTGLRSSVLELQSCPFDGSQVTDFVLCPEQGALAVCVGHARLYSVVAEKPAFGESWRLAPTPKHGVRVTAFVKKKTLDNEVCATRNWKLLLDVSDEAVNSHVMWMGEDWRIVFCYGAGGLQRVVAFEMGTDGAFSPIVEQADLSSHGSFVLMHDTRSTLQCAPAFLFADNTATLTFGAASRRLLAEVMEQTGRRAAETLSQLNRWQAMAVDLSVLLRGVRHRQLDMLAFFLKAKEDAMELQLTRSRPKDTGDAQQLELLASEVNAWDSVLTELGHAVVEALGDLSSKHFSQQLLQLVLSSVVRLLAMALPRELNDTWPERVQLRKRMSVMLAGHLGKLRSQIHHGSVLAAAVPELKDAAQADASEWDKLTEKELMLRCVHQGNIPEGQYHLLRRRAGSVEPLLSTFQEVALREALCRLREDDFPEAEWLLTNVGFFARVKMRELLLLSRDRRIRRVLRRELVRMAVLTEADSSLLDKVAAIEECFPQEDVGQASWLASQKRAQLRLVTPQASELAVAVLKPAELPPRQAPKAGGDTWLLDESPYFRVAPAWLHYWDADTLDRVLAEGRAGSPLLDMQELGGDNWRLLLEHGHLERALSGPLPSEALEASSCMSHVHRLLLAALARGGQIHEDVLEDFGDLLGLMGESGLKPSQLARVCHGPVTPEDFVHRMVHYCLRERLHHFLYLFLTDLRPELPPLCPQCNGCFVQALRDHWQWLDDPQDVAKAERAIASLARSLPAKGSAVPRILEPAVVGADLQDISLYQLLQKGTPFEIAGLFAWQTTNACQVDSGGSLMPHFSLPSLRDKFGLREELSFPYYLHKGRPCAAAFKFLAQELLLHGSLSTKRIRSACHRALRLAEQRPHDARAVAASVAFAEILGMDTRSFRVTLRTTRLALTSSPGCQSRLSDILRVLRRSKDAMQEVLLQATLALEEQLTGAASFSPMDEKCSSGILLLLRFATEHGFEVPDVALRLCAAANDWLGFLLWAQLLQCPRTQVLDLVQLFQDGCLREHLSKALSLVSSLQPSKHHRRDLRASLYVRIGLQDPSTRGHDPSESAAEQDAVSVCSSEGIDAAAALNTEPHYSTDLVELMLQCHCGGGATPGSSGMAWQSLLRAAVVLKHQTPCVIAGCCPDAPLGSCLALWLKLCSGGEEAEMPEDGRLEFTLAELQQTMEVVVKRGRLRTLLLGLQLFQPRSPLVTLVEFLVAFFLERNRKRAEECLERLKRKFTKMEAASTPGVGLTSLPWLRETSQRLLGRAFGLCRSHLDLAALAAFCDKLHGGDKMPHLHKLLASLSPASLLHEFDWSSLWGQPGERAATLARLVSQLNQTGRFSEALALARAAGLPLADVALQQLELRFEDRSATANDTFWEDCDQVLQENSVDPALAFDFLKGKTLQVEGARELYEVGHRALQWLRRSAAWGSPEAQRWEARVWLWCVRSGAEPTLEANPKPDPLVAQPPPSPGACVTLDDSQQVAATEKLIDELLSRGHFCKACQLAFLFGCSTSDLELLLTCIRLAQGTQSSDNLDPRIQLRISEPAPVLAHRLSVVASMSAVDLKSLEGGEDSPERKTLQLLERITRAAKKSQQLCQRVLLTFTLSLNLGCSYSVLALESDPVALLGNLVGHSLAKMEVQKRASGQRDGARQCCSADFALAKKLVAVFGIPDDRVANFLFHMAMDAIRGNAVASKAGILEVWDLALELCPDPSLLGNLLLRARVHDLRALSSNPKALSVEVELCVRAHSCFLEACSMEGISRVLHRCHRLTPCLVAGRHFSLLVSLLTGMARYSEMAYVFDLLLQNHHFELLFQRGMDKVPYLRVALLDYLKHRASTDPDLYSMLTLNFNMHREIAESLELTALTKMKKLVTDGPMAWSPQEQRALETVLQDLADAAESYVKAECLLRAQSCGRKAQLVALQLRYFASQLVLINLEPSAAMTQVARHPNFFEAHIVAEAYGLQGWHSAALFSRVLLDGDWGYLADFCSVCELTSQHAHELALRYQNEAAGNAKCRDALEKLLERLPCVLSRLQLAQKLGFARLASQTLEAHPYLRDYLDQRT

Target ORF information:

RefSeq Version	XM_029973179.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis spatacsin (LOC8037553), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029973179.1

ORF Insert Sequence:

ATGACTCTAA CTCTGCTCAA GAAACTCAAA GGCCCTCGGT CCGAGCCACG CGCACACATC 
GTCAGTCCGT CCCCGCTGCT AGTGTCGTAC TGTTTCATCC ACGAGTCGAA CCTGAAAGTC 
GTCGACCTGA AAACGGACCG TGTCATTTCG TCCTTACGCG ACGTTCAGGA CTGTATCTGG 
AGGGACGGTC TCGCCGACGC CGACGAACTT GCGGTCGTCT CGAGCGGACA TGAAGTTTCC 
CTGGTGCGAT ACGACGGGCA CGGCTGTACT TACAAGCAGA GATCTCTGCG TTGGAACTCC 
GTCCAAAGAC TGGCGAAGGT CGAATGGGTG CTCTGCCACG GTGACAACTT CGTCTTCCTG 
ATCAACCGGT CTCTCTTGCT GTGCACTCTT TTGATCGGAG ATGCTCTCGA GACGGTGTGG 
CACTCAGACT TGGGTTGCGA GGTGGTGCGG GTGAGGGTGA TAGACTCCGA GGTGTACGTG 
CTTACGTCGC AAGCAAGCAT TGTAGGGTAC AGCCTCCAAA CTGGGTTGCG ATCCTCTGTT 
CTGGAGCTAC AGTCCTGCCC CTTCGATGGC AGCCAGGTCA CAGACTTCGT CCTGTGCCCC 
GAACAAGGTG CCCTCGCAGT ATGCGTCGGG CACGCAAGGC TTTACTCAGT CGTGGCGGAG 
AAGCCCGCCT TCGGCGAGAG CTGGCGCCTC GCGCCGACCC CTAAACATGG GGTGAGGGTC 
ACGGCGTTTG TGAAGAAAAA AACGCTCGAC AATGAAGTGT GTGCCACCCG GAATTGGAAG 
CTGCTTTTGG ACGTGTCCGA CGAAGCGGTG AACAGTCACG TCATGTGGAT GGGCGAAGAC 
TGGAGGATTG TGTTTTGCTA CGGCGCAGGA GGACTGCAGA GGGTCGTGGC TTTCGAGATG 
GGTACAGATG GCGCGTTCTC GCCCATTGTC GAGCAGGCGG ATCTTTCGAG CCACGGGAGC 
TTTGTGTTAA TGCACGACAC GAGGAGTACC CTGCAGTGCG CCCCGGCATT CCTGTTTGCC 
GACAACACCG CCACCCTCAC CTTTGGCGCC GCCAGCCGTC GACTGTTGGC TGAGGTCATG 
GAGCAGACTG GGCGACGTGC TGCGGAGACG CTGTCGCAGC TCAACCGGTG GCAGGCGATG 
GCCGTGGACT TGTCCGTTCT GCTCCGGGGA GTTCGACACC GGCAGCTCGA CATGCTGGCT 
TTTTTTCTCA AGGCCAAGGA GGACGCCATG GAGCTCCAGC TGACGAGAAG TCGGCCCAAG 
GACACCGGGG ATGCGCAGCA GCTCGAGTTG CTCGCCTCGG AAGTGAACGC CTGGGACAGC 
GTCCTGACCG AACTGGGCCA TGCAGTCGTG GAAGCCTTAG GCGATCTCTC GTCCAAACAC 
TTCTCACAGC AGCTGCTTCA GCTTGTCCTG TCGAGTGTTG TTCGTCTTCT GGCGATGGCA 
TTGCCTCGAG AGTTGAACGA CACTTGGCCC GAGAGAGTTC AGCTTCGAAA GCGGATGTCT 
GTCATGCTGG CAGGCCATCT GGGGAAGCTC CGAAGCCAGA TCCATCACGG ATCGGTTTTG 
GCAGCTGCCG TTCCGGAGCT GAAGGATGCA GCACAGGCTG ATGCTTCTGA ATGGGACAAG 
CTCACCGAGA AAGAACTCAT GCTGCGGTGC GTGCACCAAG GGAACATTCC GGAGGGCCAG 
TACCACCTGC TGCGTCGCAG GGCGGGCTCC GTCGAGCCCC TGCTGAGCAC CTTTCAGGAG 
GTTGCTTTGA GGGAGGCATT GTGCCGGCTC CGTGAGGATG ACTTTCCCGA GGCGGAATGG 
CTCTTGACAA ACGTGGGGTT CTTTGCCAGA GTTAAGATGC GGGAGCTGCT CCTCCTGTCG 
CGCGACCGGC GGATTCGGCG GGTGCTGAGA CGGGAGCTGG TGAGGATGGC GGTCCTGACC 
GAGGCGGACT CCAGTCTTCT CGACAAAGTG GCGGCCATCG AAGAGTGTTT TCCCCAGGAA 
GATGTGGGAC AGGCATCATG GTTGGCCTCG CAGAAGCGAG CGCAGCTTAG GTTGGTCACT 
CCACAGGCTT CTGAGCTTGC TGTGGCGGTA CTGAAACCGG CGGAGTTGCC ACCAAGGCAG 
GCACCGAAGG CAGGTGGCGA CACTTGGCTC CTGGACGAGT CGCCCTATTT CCGAGTGGCG 
CCTGCCTGGC TTCATTACTG GGACGCCGAC ACCTTAGACA GAGTTTTGGC CGAGGGGCGT 
GCCGGCTCGC CTCTCCTGGA TATGCAAGAG CTTGGAGGCG ACAATTGGCG TCTACTTCTG 
GAGCACGGCC ATCTGGAGCG TGCCCTGTCG GGTCCACTTC CCAGCGAAGC TCTTGAGGCG 
TCCTCCTGCA TGTCCCACGT GCATCGTCTG CTGTTGGCTG CACTGGCAAG GGGTGGCCAG 
ATACATGAGG ACGTTCTGGA GGACTTTGGG GACCTCCTGG GTCTCATGGG GGAGTCTGGC 
CTGAAGCCCT CGCAGCTGGC CAGGGTCTGC CACGGCCCCG TGACCCCCGA GGACTTTGTC 
CATCGCATGG TGCACTACTG CCTCCGGGAG AGGCTGCACC ACTTCCTGTA CCTGTTTCTC 
ACGGACCTCA GGCCAGAGCT TCCTCCACTG TGCCCCCAGT GCAACGGCTG CTTTGTCCAG 
GCACTTCGGG ATCATTGGCA GTGGCTGGAC GACCCACAAG ATGTTGCAAA GGCAGAGCGT 
GCCATTGCAT CCTTGGCTCG AAGCCTGCCA GCAAAGGGCA GCGCAGTGCC GAGGATATTG 
GAGCCGGCTG TTGTGGGTGC CGACCTCCAG GACATCTCTC TGTACCAGCT GCTGCAGAAA 
GGAACCCCCT TTGAGATAGC GGGCCTGTTT GCCTGGCAGA CTACCAACGC CTGTCAGGTG 
GACTCTGGCG GGTCGCTGAT GCCCCACTTC TCCCTGCCGT CCCTGCGCGA CAAGTTTGGG 
CTGCGGGAGG AGCTGAGCTT CCCCTACTAC CTCCACAAGG GGCGCCCCTG TGCGGCTGCC 
TTCAAGTTCC TCGCGCAGGA GCTCCTGCTG CACGGATCTC TCTCCACCAA AAGGATACGC 
TCAGCCTGTC ATCGAGCTTT GCGGTTGGCG GAACAACGCC CCCACGACGC CCGTGCCGTT 
GCTGCGAGCG TCGCTTTTGC GGAGATTCTG GGCATGGATA CTCGCAGCTT CCGAGTCACC 
CTCCGGACAA CTCGGCTGGC CTTGACATCT TCTCCTGGCT GCCAAAGTCG ACTCAGCGAC 
ATCCTGAGAG TGCTTCGTCG AAGCAAGGAC GCCATGCAGG AAGTCCTGCT GCAGGCAACT 
CTTGCCTTAG AAGAACAGCT GACGGGTGCT GCGTCCTTCT CACCGATGGA CGAGAAGTGC 
AGCAGCGGAA TCTTGCTGCT TTTACGGTTT GCAACTGAGC ACGGGTTTGA GGTGCCCGAC 
GTCGCGCTGC GATTGTGCGC AGCTGCGAAC GACTGGCTCG GTTTTCTGCT CTGGGCACAG 
CTCCTTCAGT GCCCCAGGAC ACAGGTGCTG GATCTAGTGC AGCTCTTCCA AGATGGCTGC 
CTCCGTGAGC ACTTGAGCAA GGCGCTTTCA CTAGTGAGCT CCCTGCAGCC GAGCAAGCAT 
CACCGCCGGG ACCTCCGGGC ATCTCTTTAT GTGCGCATTG GACTTCAGGA TCCCTCCACC 
AGGGGGCACG ACCCTTCAGA ATCGGCGGCT GAACAAGATG CTGTGTCAGT GTGCAGCAGT 
GAAGGCATCG ACGCTGCTGC TGCCCTTAAC ACCGAACCGC ACTACTCGAC GGACCTCGTT 
GAGCTGATGC TGCAGTGCCA TTGCGGCGGC GGGGCTACCC CCGGGTCTTC GGGCATGGCC 
TGGCAGAGCC TGCTGCGTGC TGCGGTGGTC CTGAAGCACC AGACGCCCTG CGTCATTGCC 
GGCTGCTGTC CCGATGCCCC CCTGGGCAGC TGCCTGGCAT TGTGGCTCAA ACTTTGTTCC 
GGCGGAGAGG AAGCGGAAAT GCCCGAGGAC GGTCGCCTTG AATTCACCCT GGCGGAGCTG 
CAGCAGACGA TGGAGGTGGT GGTCAAGAGG GGCCGACTGC GGACTCTTCT CCTGGGACTC 
CAGCTCTTCC AGCCTAGAAG CCCTCTAGTC ACACTCGTCG AGTTCCTGGT CGCATTCTTT 
CTGGAGCGGA ACCGAAAACG AGCCGAGGAA TGCCTTGAGC GCCTCAAGCG CAAGTTCACC 
AAGATGGAAG CCGCTTCAAC ACCAGGGGTC GGCCTGACCT CTCTGCCTTG GCTACGTGAG 
ACCTCCCAGC GTCTGTTGGG ACGCGCTTTT GGCCTGTGCC GTTCCCACCT CGACCTGGCA 
GCCCTCGCGG CCTTCTGCGA CAAGCTTCAC GGGGGCGATA AGATGCCGCA CCTCCACAAA 
CTCCTCGCAT CTTTGAGTCC GGCGTCCCTG CTTCATGAGT TTGACTGGAG CTCGTTGTGG 
GGCCAACCGG GTGAAAGGGC GGCCACCCTG GCACGACTGG TGTCGCAGCT CAACCAGACG 
GGCCGTTTCT CGGAGGCGCT GGCGCTGGCT AGGGCGGCGG GACTGCCACT AGCGGACGTG 
GCGCTCCAGC AGCTGGAACT GAGGTTCGAG GATCGCTCTG CCACCGCTAA TGACACATTC 
TGGGAGGATT GCGACCAAGT GCTTCAGGAG AACTCCGTGG ATCCTGCACT CGCCTTCGAC 
TTTCTCAAGG GCAAAACGCT GCAAGTGGAA GGTGCACGGG AACTGTACGA GGTGGGCCAC 
CGTGCGCTGC AGTGGCTGCG CCGCAGCGCT GCCTGGGGCT CGCCCGAGGC CCAGCGCTGG 
GAGGCGCGGG TGTGGCTATG GTGTGTGCGC AGCGGGGCAG AGCCCACCCT CGAAGCGAAC 
CCCAAGCCAG ACCCCCTGGT GGCACAGCCC CCTCCCAGCC CGGGGGCCTG CGTCACCCTG 
GACGACTCGC AGCAAGTGGC GGCGACGGAA AAGTTAATTG ACGAGCTGCT GTCTCGGGGC 
CACTTCTGCA AGGCTTGCCA GCTGGCTTTC CTGTTCGGCT GCTCCACAAG CGACCTGGAA 
CTCTTGCTGA CATGCATCCG TCTAGCACAA GGAACGCAGT CGTCCGACAA CCTTGATCCA 
CGAATCCAAC TTCGGATCAG CGAACCAGCA CCAGTTCTGG CCCACAGGCT TTCAGTGGTT 
GCATCTATGT CTGCTGTGGA CTTGAAGTCC TTGGAAGGTG GCGAGGACTC ACCGGAGAGA 
AAGACACTCC AACTCCTGGA GCGCATCACC AGAGCAGCGA AGAAGAGCCA GCAGCTGTGT 
CAGCGTGTGC TGCTCACCTT CACCCTTTCA CTGAACCTGG GGTGCAGCTA CTCGGTCCTG 
GCCCTGGAGT CGGACCCCGT GGCCCTTTTG GGCAACCTGG TGGGCCATAG CCTGGCCAAG 
ATGGAGGTCC AGAAGCGGGC GTCTGGCCAG AGGGACGGCG CCCGCCAGTG CTGCTCTGCG 
GACTTTGCCC TGGCCAAGAA ACTGGTGGCC GTCTTCGGGA TCCCGGACGA CCGGGTGGCA 
AACTTTCTCT TCCACATGGC CATGGACGCT ATCCGAGGAA ACGCAGTTGC AAGTAAAGCG 
GGCATCTTGG AGGTGTGGGA CTTGGCCTTG GAGCTCTGCC CCGACCCTTC GCTACTGGGG 
AACCTTTTGC TCAGGGCTAG GGTTCACGAC CTGCGCGCTC TGAGCAGCAA CCCCAAAGCG 
CTGTCTGTGG AGGTGGAGCT GTGCGTGCGC GCCCACAGCT GCTTCCTGGA GGCGTGCAGC 
ATGGAGGGCA TCTCGCGGGT GCTCCACCGC TGCCACCGTC TCACGCCCTG CTTGGTGGCG 
GGGCGGCACT TCTCGCTGCT CGTGTCTTTG CTCACTGGCA TGGCGCGCTA CAGCGAGATG 
GCCTACGTCT TTGACCTGCT CCTGCAGAAC CACCACTTTG AGCTGCTCTT CCAGCGGGGC 
ATGGACAAGG TGCCTTACCT GCGGGTGGCG CTTCTAGACT ACCTGAAACA CCGCGCCAGC 
ACCGACCCAG ATCTCTACTC GATGCTCACC CTCAACTTCA ACATGCATCG CGAGATTGCC 
GAGAGCCTCG AACTGACTGC ACTGACCAAG ATGAAGAAGC TCGTCACGGA CGGACCAATG 
GCATGGAGTC CCCAAGAGCA GCGGGCGCTG GAAACGGTGC TTCAAGACCT GGCAGACGCA 
GCCGAGAGCT ACGTCAAGGC GGAGTGCTTG CTGCGGGCGC AGTCGTGCGG CAGGAAGGCA 
CAGCTGGTTG CCCTGCAGCT ACGCTACTTC GCGTCACAGC TGGTGCTCAT CAACCTGGAG 
CCGTCAGCCG CCATGACGCA GGTGGCACGT CACCCAAACT TCTTTGAGGC ACACATAGTG 
GCGGAGGCGT ACGGCCTGCA GGGCTGGCAC AGCGCGGCCC TCTTCAGCAG GGTGCTCCTG 
GACGGCGACT GGGGCTACCT GGCCGACTTC TGCTCCGTCT GCGAGCTCAC CTCTCAGCAC 
GCACACGAGC TTGCACTCAG GTACCAGAAC GAAGCGGCCG GAAACGCAAA ATGTCGGGAT 
GCGCTCGAGA AGCTCCTGGA ACGTCTCCCG TGCGTGCTGT CTCGTCTGCA GCTGGCCCAG 
AAACTAGGCT TTGCACGCCT TGCGTCGCAG ACTCTCGAGG CACACCCTTA CCTTCGAGAC 
TACCTCGACC AGCGGACGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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1-732-210-0262

LOC8037553 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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