LOC8039200 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC8039200
Entrez Gene ID	8039200
Full Name	tuberin, putative
Gene Type	protein-coding
Organism	Ixodes scapularis(black-legged tick)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029982219.1	XP_029838079.1	tuberin isoform X2
XM_002413207.1	XP_002413252.1	tuberin, putative
XM_029982218.1	XP_029838078.1	tuberin isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC8039200 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC8039200 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OIa09117	XM_002413207.1 Latest version!	Ixodes scapularis tuberin, putative, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OIa24904	XM_029982218.1 Latest version!	Ixodes scapularis tuberin (LOC8039200), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIa24905	XM_029982219.1 Latest version!	Ixodes scapularis tuberin (LOC8039200), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC8039200 ( XM_002413207.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_002413252.1 Ixodes scapularis tuberin, putative, mRNA.

Price & Availability↑

CloneID	OIa09117
Clone ID Related Accession (Same CDS sequence)	XM_002413207.1
Accession Version	XM_002413207.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2009-06-22
Organism	Ixodes scapularis(black-legged tick)
Product	tuberin, putative
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002801145). COMPLETENESS: incomplete on both ends.

ATGTCGAAAA CAAGTCGCGA GAAAGAAAAG CCGCTGGAGC GCCTCAAGTC CCTCTTCGGC 
CTGGGCAAAG GCGGATCCTC CTCGGGGCCG GGTTTCGCCG TCCCCAAAAT CGAGGAGTTC 
GCCATCACGC CGGAGATCGT CCGGGATATC GGAAATGAGA GCAGCATCTC GCACCGCACG 
AAATCCATCA AGGAACTCGT GGACATTGTA CGAACCAAAA GGCTCCAAGA GGGAGTTCCG 
GAGACCCTGT ACACGCAGAC CTGCGACCTG CTGCACCCCG CGCACGGGCA GGACGTGCGG 
CACACTGTAC TCTACTTCTA CTGCTGCCTC ATTCAGGGAC AGTTTGAGCG CCTGGACATC 
TCGCGGACGC ACTTCTTCAA CCTGATCCAG GACTTGCGAC ACCCAGAGGA CCTCGCTCTG 
AGACTGGACC TGCTGAAAGT GCTCACCGAC AAGGGAAAGT GTCTAAGCCA CTTCGAAGAC 
AAAGCACCCC AGTTCCTGCT TCAATGGATG CCAGAGGTGT TGAGTGCCGG CAGGGCGTTC 
GACTTCCTGT CTCTCCTGAT CAACGTCATC AAGTTCAACT CCTCCTTCCT GGACAAAGAA 
GTGGTTGCCG GATTTGTGCA GGCCAACTAC AGAGACTACA CCCTGCTCAA AGGAGCGGTG 
TTCTTCGTGG GTGCAGCGCT GTGGAGCCAC AAGGCGGTGC CGACTCTCAA ACACACGCCT 
GCCGCGGTGC TGCCTTCCAT ATACAGGGCC CTGGACTGCA AGCACCCCTC TGTGGCCTAC 
GAGGCGGCCG TGGCTCTTCA GTGGCTGATC GGCGATCCGC CCAAGCCGAT CACTCCCTTC 
GTCTGGAAGT TTGTCCTTGA TGTCCTCGAG AAACTGGTTT GCCTCCCCGA GGTGGAGCAG 
GCTTCTGCCA GCTCACAGCA GTTGCAGCAG ATGGTTCATG ACATTGTGTC CAAGGTGGAA 
AAATCGTACG ACGAAGGGAC GTACAAGGGT TGCCCCCAGC GCCTCTTCAA CATTGTGGCA 
AAGTGCGCCT CCCTCAGGCC CGAGGCTTCC CTGCAGCGTC TGATCCAGTA CTGTGCGGAG 
AAGGCGCAGG TCCCCACCCT GAACTGGGTG ACCAACCTGT CGGAGATCCT GGAGAAGTAC 
TTCAAGGCGG AGCCGCGGCC CTCTGTCCGC CTCTACACGC TCAACGTCGT CTCTTCCGTG 
CTCAGCAACA ACAGGCACCT CTACGAGGCA AGTGGCGGGA ACCCGAAAGC ACGAGACGCA 
GGGGATTCGA ACGCAGTTCG GCCAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002413252.1
CDS	1..1290
Translation	MSKTSREKEKPLERLKSLFGLGKGGSSSGPGFAVPKIEEFAITPEIVRDIGNESSISHRTKSIKELVDIVRTKRLQEGVPETLYTQTCDLLHPAHGQDVRHTVLYFYCCLIQGQFERLDISRTHFFNLIQDLRHPEDLALRLDLLKVLTDKGKCLSHFEDKAPQFLLQWMPEVLSAGRAFDFLSLLINVIKFNSSFLDKEVVAGFVQANYRDYTLLKGAVFFVGAALWSHKAVPTLKHTPAAVLPSIYRALDCKHPSVAYEAAVALQWLIGDPPKPITPFVWKFVLDVLEKLVCLPEVEQASASSQQLQQMVHDIVSKVEKSYDEGTYKGCPQRLFNIVAKCASLRPEASLQRLIQYCAEKAQVPTLNWVTNLSEILEKYFKAEPRPSVRLYTLNVVSSVLSNNRHLYEASGGNPKARDAGDSNAVRPV

Target ORF information:

RefSeq Version	XM_002413207.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis tuberin, putative, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002413207.1

ORF Insert Sequence:

ATGTCGAAAA CAAGTCGCGA GAAAGAAAAG CCGCTGGAGC GCCTCAAGTC CCTCTTCGGC 
CTGGGCAAAG GCGGATCCTC CTCGGGGCCG GGTTTCGCCG TCCCCAAAAT CGAGGAGTTC 
GCCATCACGC CGGAGATCGT CCGGGATATC GGAAATGAGA GCAGCATCTC GCACCGCACG 
AAATCCATCA AGGAACTCGT GGACATTGTA CGAACCAAAA GGCTCCAAGA GGGAGTTCCG 
GAGACCCTGT ACACGCAGAC CTGCGACCTG CTGCACCCCG CGCACGGGCA GGACGTGCGG 
CACACTGTAC TCTACTTCTA CTGCTGCCTC ATTCAGGGAC AGTTTGAGCG CCTGGACATC 
TCGCGGACGC ACTTCTTCAA CCTGATCCAG GACTTGCGAC ACCCAGAGGA CCTCGCTCTG 
AGACTGGACC TGCTGAAAGT GCTCACCGAC AAGGGAAAGT GTCTAAGCCA CTTCGAAGAC 
AAAGCACCCC AGTTCCTGCT TCAATGGATG CCAGAGGTGT TGAGTGCCGG CAGGGCGTTC 
GACTTCCTGT CTCTCCTGAT CAACGTCATC AAGTTCAACT CCTCCTTCCT GGACAAAGAA 
GTGGTTGCCG GATTTGTGCA GGCCAACTAC AGAGACTACA CCCTGCTCAA AGGAGCGGTG 
TTCTTCGTGG GTGCAGCGCT GTGGAGCCAC AAGGCGGTGC CGACTCTCAA ACACACGCCT 
GCCGCGGTGC TGCCTTCCAT ATACAGGGCC CTGGACTGCA AGCACCCCTC TGTGGCCTAC 
GAGGCGGCCG TGGCTCTTCA GTGGCTGATC GGCGATCCGC CCAAGCCGAT CACTCCCTTC 
GTCTGGAAGT TTGTCCTTGA TGTCCTCGAG AAACTGGTTT GCCTCCCCGA GGTGGAGCAG 
GCTTCTGCCA GCTCACAGCA GTTGCAGCAG ATGGTTCATG ACATTGTGTC CAAGGTGGAA 
AAATCGTACG ACGAAGGGAC GTACAAGGGT TGCCCCCAGC GCCTCTTCAA CATTGTGGCA 
AAGTGCGCCT CCCTCAGGCC CGAGGCTTCC CTGCAGCGTC TGATCCAGTA CTGTGCGGAG 
AAGGCGCAGG TCCCCACCCT GAACTGGGTG ACCAACCTGT CGGAGATCCT GGAGAAGTAC 
TTCAAGGCGG AGCCGCGGCC CTCTGTCCGC CTCTACACGC TCAACGTCGT CTCTTCCGTG 
CTCAGCAACA ACAGGCACCT CTACGAGGCA AGTGGCGGGA ACCCGAAAGC ACGAGACGCA 
GGGGATTCGA ACGCAGTTCG GCCAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8039200 ( XM_029982218.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029838078.1 Ixodes scapularis tuberin (LOC8039200), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OIa24904
Clone ID Related Accession (Same CDS sequence)	XM_029982218.1
Accession Version	XM_029982218.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021919882.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAA CAAGTCGCGA GAAAGAAAAG CCGCTGGAGC GCCTCAAGTC CCTCTTCGGC 
CTGGGCAAAG GCGGATCCTC CTCGGGGCCG GGTTTCGCCG TCCCCAAAAT CGAGGAGTTC 
GCCATCACGC CGGAGATCGT CCGGGATATC GGAAATGAGA GCAGCGTCTC GCACCGCACG 
AAATCCATCA AGGAACTCGT GGACATTGTA CGAACCAAAA GGCTCCAAGA GGGAGTTCCG 
GAGACCCTGT ACACGCAGAC CTGCGACCTG CTGCACCCCG CGCACGGGCA GGACGTGCGG 
CACACTGTAC TCTACTTCTA CTGCTGCCTC ATTCAGGGAC AGTTTGAGCG CCTGGACATC 
TCGCGGACGC ACTTCTTCAA CCTGATCCAG GACTTGCGAC ACCCAGAGGA CCTCGCTCTG 
AGACTGGACC TGCTGAAAGT GCTCACCGAC AAGGGAAAGT GTCTAAGCCA CTTCGAAGAC 
AAAGCACCCC AGTTCCTGCT TCAATGGATG CCAGAGGTGT TGAGTGCCGG CAGGGCGTTC 
GACTTCCTGT CTCTCCTGAT CAACGTCATC AAGTTCAACT CCTCCTTCCT GGACAAAGAA 
GTGGTTGCCG GATTTGTGCA AAATACCTGC ATCCTTTGCT GTAGGACCAA CTCTGAAAGC 
GACGTCAGGC TGGGACTGGA GGTGCTGGAC AGCGTAGTGG GCTACAGCCT ACTCTCATCC 
GAGGTGCTAC CTCACCTGGT GCCTACCCTG TGCCGGACGG TGGTCCTTGC CAAGCTGTCC 
GAGCCCAGCT GGAGGACGAT GCGCAACCTC CAGGGGACTC ATCTTGGCCA CAACGTCATC 
TCCTACCTCT GCCGGATCCT CGAAGACAGG GCCAACTACA GAGACTACAC CCTGCTCAAA 
GGAGCGGTGT TCTTCGTGGG TGCAGCGCTG TGGAGCCACA AGGCGGTGCC GACGCTCAAA 
CACACGCCTG CCGCGGTGCT GCCTTCCATA TACAGGGCCC TGGACTGCAA GCACCCCTCT 
GTGGCCTACG AGGCGGCCGT GGCTCTTCAG TGGCTGATCG GCGATCCGCC CAAGCCGATC 
ACTCCCTTCG TCTGGAAGTT TGTCCTTGAT GTCCTCGAGA AACTGGTTTG CCTCCCCGAG 
GTGGAGCAGG CTTCTGCCAG CTCACAGCAG TTGCAGCAGA TGGTTCATGA CATTGTGTCC 
AAGGTGGAAA AATCGTACGA CGAAGGGACG TACAAGGGCT GCCCCCAGCG CCTCTTCAAC 
ATTGTGGCAA AGTGCGCCTC CCTCAGGCCC GAGGCTTCCC TGCAGCGTCT GATCCAGTAC 
TGTGCGGAGA AGGCGCAGGT CCCCACCCTG AACTGGGTGA CCAACCTGTC GGAGATCCTG 
GAGAAGTACT TCAAGGCGGA GCCGCGGCCT TCTGTCCGCC TCTACACGCT CAACGTCGTC 
TCTTCCGTGC TCAGCAACAA CAGGCACCTC TACGAGGAGG AACTGATGAA GAAGGTGCTG 
CTCCCCCACC TCGGCGCGCT GGAAACAGAT CCAGATCCGG CCGTGGTGAC CGCGGCGGTC 
CAGCTGTTGA CCGACTTCTT TCAGGACAGC GGCAGCCAGC AGAACAATGA AGTTCTGGAC 
ATCATCGAAA AGGTGCTGAA CCACCCGACT TGGTCGACAA AGCCGAACGG CGCTGAGGAG 
GTGCTTCCCG TGGAGACGGC GGCCAGAGGT CTCATTGACG TTTTTCGGAA GAAGATGTGG 
CTGAGCCCTC AGTGTGCCGT GGCCACTTTC CGTCTGCTAG TTGACCACGT ACGTCGTCAA 
TACCGGCAGG ACCCCTGTCA GCTGCTGGCG CCCGTACGCC TAGTGATCAT GAAGTTCCTC 
GTGAGCCTGC GGACCAATGC CCTGTACCAG ATCGGCATGG ACGAAGGGTC GGAAGAGGGA 
ATTCGCTACA GCCCCCACCT CCTCTGCCCC TTCAGGGAAG GCTCAGCCAT GAAGCAGTGC 
TCCCCAGCAA GTGCCATTAC AAACAGCGGC GTGGTGGCTG CATCGGCACA ACCCCACATT 
GCTCACTTGG GTGTGGCGGA AGCCTTTGAC ACCATCATCG CAAGCATCAG GGAGGAGAGC 
GACTGGCCAG TCCTGTCGAC AGTCCTGCAG GGTCTTCCCG GCCTGCTAAA AGACAAAGCG 
CTTGTCCTGA GTGGGCAGAT GTCCTTCCGT GCACTCTGCG TCCGCCTCCG CAATCAAGCC 
AACGAGCGAA ACCGGAAGCC GCTGGACCAG ATGCACAGGG TGCCGGCCGG TGTCACGGCC 
TCTGACCTGA ACGCTCTGCT GTACCCAGCC ATGGGTGCCA TGACCATGTA CCGGACGGAA 
CTGGACAGCT CCTCCATGTG TATGGTCATC CACATGCTGG AGCACGGGCT GGCTTCCAAG 
CAGGCCTGCG TGTGCTTGCG TGCCTTGGTG CTGGCAACAC TGGAGATGAG GGACGCGATG 
GGCAAGTTGC TGCCCGAAGT GCTGCAGCGG CTCGCGCAGG TGTCGGCAAC ACTGGCGGTG 
GCCGTCCCGG TCCTCGAGTT CCTCTCAAAC CTCATCAGGA TCCCGCAGCT GTACGTCAGC 
TTTGTCGAGG AGCAGTACCG CAAGATCTTT GCCATTGCTC TGCCATACAC CAACCCCGTC 
AAGTTCAACC GCTTTGTGGT GGCGCTGGCT CATCACGTGA TTGCCATGTG GTTCCTCAAG 
TGCAGAATCA CGTTTCGGAA GGACCTTGTG CCTTACGTCA TCAAGAACCT CAAGAACAAC 
TTGGAACTCC ACGTGGAAGA CGGTGGCATG GAAGACACAA CGCAGCGGAA GAGAAGCAGC 
AGCCTCAACG AACCTATGGC GGTGGCAGCC GCGTCGCGCA GCCGAAGCGA GGTGCGCCAC 
TCTGTGGCGA GCGTCTCTTA CGCCAGCGGC TCTGGCTCTT CTAGCGACAT GGTGAAGCTA 
CAGGAGGAGC TGCTCGAGAC CTTCGTCGAC CTCATGTCTC AGTACTGCTT TGGCACCTGC 
TCCTCGCAGC CGCACAGGTC GGCCACGTCC GAGTTCATGC TGAAGCAGGG TCGCTCCATG 
ACCTGGCTGC TGGGCCACAA GATGGTGACC ATCACGACGA GTGGCTGCGG GGGCCGTGCC 
TACCGGCTGG GCATGTGTGA GCGCTGCTAC TGCTTCTGCA GGGACGCACA AGACCAACCG 
GACCCCTGCC AAGAAGGACT CCGGCCGGCG TCGGAAGTGC CACTGGCCGG TTTCCCGTCG 
GAAGAGCAGC AGGGCCAGAA CCGGCGGCGC CACCAGTCGG AGGCGGACGG GCGGAGCTCC 
CTCTCGGGGG CCGGAGCGTT TGCGTCACGC ATGCTCTCCG GCACCAAGGA CGACCTCAGC 
CTTCGGCAGG TGCCCCCAGA CAAGCGGGCC GGCATGGGGG GCGACAGCGA TCCGGGTGAC 
GGCAGGGGCG GTGGCATCCG GTTGGGCGGA GCGCATGGGT CTAGTCACCT GTCCGGAAGC 
ACTTCTGGAG AAGTGCTAAG TCACGTGTGC AGCTGCTGGT GCTGCGGCTG GGCCGAGGTG 
TACGTCCGGC GCCCCACGGG TAACACCTCC TGGGTCATGC GGCTCCAAAA TGACATGTTC 
TCCAAGACCT GCACACCCGA CTTCCCCCTG CCTGATGTGT CGGCCATCTT TCGCCAGCCC 
CAGATGATGG CCCAGGACTG GGCGGGACCC CCGGAAGAGC CTGTCGTCTG CAAACGGGAC 
CGACGTCCGA GCGAAGGAGG GCGAGTCAGA AGCGCCGGCA GCTCGGAGGA CATCTCTCCG 
AGTGCCCCTT TGGAGCGCAG CGGATCTCCC CTGCGCCGCA CAAACTCTAG TCCCGAGGTT 
CCCGCCCAGA GGCCCCCCGG AGACGGCTGC AAAACGGCCA GCCCCGGGTC GGCTCCGCAG 
AGTCCGTCGG CTATCTTCGA TCGGCTGGAC GAAGAGGGCG CCAGGCTGGC ACTGCGGAAG 
TCGCCGCGCA AGGACGACAG GTACGACTGC ATTCCCGAAG AATCGCACTG GGGTCTGCGG 
GCCCCTCCGT TCTCAAAGGC AAGGCTCAAA TTGGACCTGT CCCCGTCGGC CATGAGCGAG 
CCCCCAGTGG GGCCGGCCAC GTCGCTGCAC AGTCCCCCGC CACTGTCGCG TCTGCAAAGC 
CTGCAGGGTC GGCCCAACGA CGGGAGCAGC CCCACCCCGG CGAGCTCGTA CCGGGACCGG 
GGACACACGG TGTCCGGCAT CAGCCCCGTG ACGCACCAGG CCGCCACACC CACGGTGTCC 
TACATCGACA TGTACCGGTG CGGCATGAAC CCACGGTTCG TGTTCCTGCA CCTGTACCAC 
AACTCGTTCT TTGGGAAGCC GGGCACTGAC GAACCGATCC CTGTGCCGGA GTCAAAGTGG 
GATCGCAGCT CCAATTCCGG GAACGCGCTG GATTTTGTGC CGACCTACGA AACTCACAAG 
ATTGGCGTGG TGTACGTCGG CCCAGGACAG GCCCAGTCGG AGACGGCGAT GCTCTCGAAC 
CGGAGCGGCT CGGAGCGCTA CACTCGATTC CTGCTTGGCC TCGGACGCAC GGTGTGCTTG 
CGGGACCTGG ACACCAAGGC CACCTACACG GGAGGGCTGG ACGTGCGCGG CGCGGACGGC 
AAGTACTCCA TCAGCTGGCA GGACGACATC ATGCAGGTGA TCTTCCACGT GGCCACGCTG 
ATGCCTAACA AGGAGAACGA CCGGCAACGC AGCGACAAGA AGCGTCACAT CGGCAATGAC 
TACGTCATGG TGCTCTACAA CGACAGCTCC GAGTCCTGCG ACAGGGAAAC TTTCCGGGGC 
GAATTTCACC ATGCCTTCGT GATCATAGAA CCGTGGAAGC TGGGCAACAA TTTGGTGACT 
CTCGAGGGAA AAGCAGAGGT GATGGAGCTC TTTCGACACA GCGAGCCGCA GGTGGTTCCG 
GACAGCAGCT TGTCCCTGTA CGTTCGGCAA CTCGCATTGC ACGCCAACAT TGCGTGCAAC 
ATAGTGCTGC AAAAGACGAA GGGACCCGAC CTGTTTGTGA ACAACTGGCA CGGGCGGCTG 
CGCCACATCA ACCTCATCCG TCAGCGCCTC CTGGATGCCA GGGCCAAGGG GGAGTCTACA 
GACCCACCTG CTGCCACGGA GGACTTCACT CGGTACCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029838078.1
CDS	116..5257
Translation	MSKTSREKEKPLERLKSLFGLGKGGSSSGPGFAVPKIEEFAITPEIVRDIGNESSVSHRTKSIKELVDIVRTKRLQEGVPETLYTQTCDLLHPAHGQDVRHTVLYFYCCLIQGQFERLDISRTHFFNLIQDLRHPEDLALRLDLLKVLTDKGKCLSHFEDKAPQFLLQWMPEVLSAGRAFDFLSLLINVIKFNSSFLDKEVVAGFVQNTCILCCRTNSESDVRLGLEVLDSVVGYSLLSSEVLPHLVPTLCRTVVLAKLSEPSWRTMRNLQGTHLGHNVISYLCRILEDRANYRDYTLLKGAVFFVGAALWSHKAVPTLKHTPAAVLPSIYRALDCKHPSVAYEAAVALQWLIGDPPKPITPFVWKFVLDVLEKLVCLPEVEQASASSQQLQQMVHDIVSKVEKSYDEGTYKGCPQRLFNIVAKCASLRPEASLQRLIQYCAEKAQVPTLNWVTNLSEILEKYFKAEPRPSVRLYTLNVVSSVLSNNRHLYEEELMKKVLLPHLGALETDPDPAVVTAAVQLLTDFFQDSGSQQNNEVLDIIEKVLNHPTWSTKPNGAEEVLPVETAARGLIDVFRKKMWLSPQCAVATFRLLVDHVRRQYRQDPCQLLAPVRLVIMKFLVSLRTNALYQIGMDEGSEEGIRYSPHLLCPFREGSAMKQCSPASAITNSGVVAASAQPHIAHLGVAEAFDTIIASIREESDWPVLSTVLQGLPGLLKDKALVLSGQMSFRALCVRLRNQANERNRKPLDQMHRVPAGVTASDLNALLYPAMGAMTMYRTELDSSSMCMVIHMLEHGLASKQACVCLRALVLATLEMRDAMGKLLPEVLQRLAQVSATLAVAVPVLEFLSNLIRIPQLYVSFVEEQYRKIFAIALPYTNPVKFNRFVVALAHHVIAMWFLKCRITFRKDLVPYVIKNLKNNLELHVEDGGMEDTTQRKRSSSLNEPMAVAAASRSRSEVRHSVASVSYASGSGSSSDMVKLQEELLETFVDLMSQYCFGTCSSQPHRSATSEFMLKQGRSMTWLLGHKMVTITTSGCGGRAYRLGMCERCYCFCRDAQDQPDPCQEGLRPASEVPLAGFPSEEQQGQNRRRHQSEADGRSSLSGAGAFASRMLSGTKDDLSLRQVPPDKRAGMGGDSDPGDGRGGGIRLGGAHGSSHLSGSTSGEVLSHVCSCWCCGWAEVYVRRPTGNTSWVMRLQNDMFSKTCTPDFPLPDVSAIFRQPQMMAQDWAGPPEEPVVCKRDRRPSEGGRVRSAGSSEDISPSAPLERSGSPLRRTNSSPEVPAQRPPGDGCKTASPGSAPQSPSAIFDRLDEEGARLALRKSPRKDDRYDCIPEESHWGLRAPPFSKARLKLDLSPSAMSEPPVGPATSLHSPPPLSRLQSLQGRPNDGSSPTPASSYRDRGHTVSGISPVTHQAATPTVSYIDMYRCGMNPRFVFLHLYHNSFFGKPGTDEPIPVPESKWDRSSNSGNALDFVPTYETHKIGVVYVGPGQAQSETAMLSNRSGSERYTRFLLGLGRTVCLRDLDTKATYTGGLDVRGADGKYSISWQDDIMQVIFHVATLMPNKENDRQRSDKKRHIGNDYVMVLYNDSSESCDRETFRGEFHHAFVIIEPWKLGNNLVTLEGKAEVMELFRHSEPQVVPDSSLSLYVRQLALHANIACNIVLQKTKGPDLFVNNWHGRLRHINLIRQRLLDARAKGESTDPPAATEDFTRYL

Target ORF information:

RefSeq Version	XM_029982218.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis tuberin (LOC8039200), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029982218.1

ORF Insert Sequence:

ATGTCGAAAA CAAGTCGCGA GAAAGAAAAG CCGCTGGAGC GCCTCAAGTC CCTCTTCGGC 
CTGGGCAAAG GCGGATCCTC CTCGGGGCCG GGTTTCGCCG TCCCCAAAAT CGAGGAGTTC 
GCCATCACGC CGGAGATCGT CCGGGATATC GGAAATGAGA GCAGCGTCTC GCACCGCACG 
AAATCCATCA AGGAACTCGT GGACATTGTA CGAACCAAAA GGCTCCAAGA GGGAGTTCCG 
GAGACCCTGT ACACGCAGAC CTGCGACCTG CTGCACCCCG CGCACGGGCA GGACGTGCGG 
CACACTGTAC TCTACTTCTA CTGCTGCCTC ATTCAGGGAC AGTTTGAGCG CCTGGACATC 
TCGCGGACGC ACTTCTTCAA CCTGATCCAG GACTTGCGAC ACCCAGAGGA CCTCGCTCTG 
AGACTGGACC TGCTGAAAGT GCTCACCGAC AAGGGAAAGT GTCTAAGCCA CTTCGAAGAC 
AAAGCACCCC AGTTCCTGCT TCAATGGATG CCAGAGGTGT TGAGTGCCGG CAGGGCGTTC 
GACTTCCTGT CTCTCCTGAT CAACGTCATC AAGTTCAACT CCTCCTTCCT GGACAAAGAA 
GTGGTTGCCG GATTTGTGCA AAATACCTGC ATCCTTTGCT GTAGGACCAA CTCTGAAAGC 
GACGTCAGGC TGGGACTGGA GGTGCTGGAC AGCGTAGTGG GCTACAGCCT ACTCTCATCC 
GAGGTGCTAC CTCACCTGGT GCCTACCCTG TGCCGGACGG TGGTCCTTGC CAAGCTGTCC 
GAGCCCAGCT GGAGGACGAT GCGCAACCTC CAGGGGACTC ATCTTGGCCA CAACGTCATC 
TCCTACCTCT GCCGGATCCT CGAAGACAGG GCCAACTACA GAGACTACAC CCTGCTCAAA 
GGAGCGGTGT TCTTCGTGGG TGCAGCGCTG TGGAGCCACA AGGCGGTGCC GACGCTCAAA 
CACACGCCTG CCGCGGTGCT GCCTTCCATA TACAGGGCCC TGGACTGCAA GCACCCCTCT 
GTGGCCTACG AGGCGGCCGT GGCTCTTCAG TGGCTGATCG GCGATCCGCC CAAGCCGATC 
ACTCCCTTCG TCTGGAAGTT TGTCCTTGAT GTCCTCGAGA AACTGGTTTG CCTCCCCGAG 
GTGGAGCAGG CTTCTGCCAG CTCACAGCAG TTGCAGCAGA TGGTTCATGA CATTGTGTCC 
AAGGTGGAAA AATCGTACGA CGAAGGGACG TACAAGGGCT GCCCCCAGCG CCTCTTCAAC 
ATTGTGGCAA AGTGCGCCTC CCTCAGGCCC GAGGCTTCCC TGCAGCGTCT GATCCAGTAC 
TGTGCGGAGA AGGCGCAGGT CCCCACCCTG AACTGGGTGA CCAACCTGTC GGAGATCCTG 
GAGAAGTACT TCAAGGCGGA GCCGCGGCCT TCTGTCCGCC TCTACACGCT CAACGTCGTC 
TCTTCCGTGC TCAGCAACAA CAGGCACCTC TACGAGGAGG AACTGATGAA GAAGGTGCTG 
CTCCCCCACC TCGGCGCGCT GGAAACAGAT CCAGATCCGG CCGTGGTGAC CGCGGCGGTC 
CAGCTGTTGA CCGACTTCTT TCAGGACAGC GGCAGCCAGC AGAACAATGA AGTTCTGGAC 
ATCATCGAAA AGGTGCTGAA CCACCCGACT TGGTCGACAA AGCCGAACGG CGCTGAGGAG 
GTGCTTCCCG TGGAGACGGC GGCCAGAGGT CTCATTGACG TTTTTCGGAA GAAGATGTGG 
CTGAGCCCTC AGTGTGCCGT GGCCACTTTC CGTCTGCTAG TTGACCACGT ACGTCGTCAA 
TACCGGCAGG ACCCCTGTCA GCTGCTGGCG CCCGTACGCC TAGTGATCAT GAAGTTCCTC 
GTGAGCCTGC GGACCAATGC CCTGTACCAG ATCGGCATGG ACGAAGGGTC GGAAGAGGGA 
ATTCGCTACA GCCCCCACCT CCTCTGCCCC TTCAGGGAAG GCTCAGCCAT GAAGCAGTGC 
TCCCCAGCAA GTGCCATTAC AAACAGCGGC GTGGTGGCTG CATCGGCACA ACCCCACATT 
GCTCACTTGG GTGTGGCGGA AGCCTTTGAC ACCATCATCG CAAGCATCAG GGAGGAGAGC 
GACTGGCCAG TCCTGTCGAC AGTCCTGCAG GGTCTTCCCG GCCTGCTAAA AGACAAAGCG 
CTTGTCCTGA GTGGGCAGAT GTCCTTCCGT GCACTCTGCG TCCGCCTCCG CAATCAAGCC 
AACGAGCGAA ACCGGAAGCC GCTGGACCAG ATGCACAGGG TGCCGGCCGG TGTCACGGCC 
TCTGACCTGA ACGCTCTGCT GTACCCAGCC ATGGGTGCCA TGACCATGTA CCGGACGGAA 
CTGGACAGCT CCTCCATGTG TATGGTCATC CACATGCTGG AGCACGGGCT GGCTTCCAAG 
CAGGCCTGCG TGTGCTTGCG TGCCTTGGTG CTGGCAACAC TGGAGATGAG GGACGCGATG 
GGCAAGTTGC TGCCCGAAGT GCTGCAGCGG CTCGCGCAGG TGTCGGCAAC ACTGGCGGTG 
GCCGTCCCGG TCCTCGAGTT CCTCTCAAAC CTCATCAGGA TCCCGCAGCT GTACGTCAGC 
TTTGTCGAGG AGCAGTACCG CAAGATCTTT GCCATTGCTC TGCCATACAC CAACCCCGTC 
AAGTTCAACC GCTTTGTGGT GGCGCTGGCT CATCACGTGA TTGCCATGTG GTTCCTCAAG 
TGCAGAATCA CGTTTCGGAA GGACCTTGTG CCTTACGTCA TCAAGAACCT CAAGAACAAC 
TTGGAACTCC ACGTGGAAGA CGGTGGCATG GAAGACACAA CGCAGCGGAA GAGAAGCAGC 
AGCCTCAACG AACCTATGGC GGTGGCAGCC GCGTCGCGCA GCCGAAGCGA GGTGCGCCAC 
TCTGTGGCGA GCGTCTCTTA CGCCAGCGGC TCTGGCTCTT CTAGCGACAT GGTGAAGCTA 
CAGGAGGAGC TGCTCGAGAC CTTCGTCGAC CTCATGTCTC AGTACTGCTT TGGCACCTGC 
TCCTCGCAGC CGCACAGGTC GGCCACGTCC GAGTTCATGC TGAAGCAGGG TCGCTCCATG 
ACCTGGCTGC TGGGCCACAA GATGGTGACC ATCACGACGA GTGGCTGCGG GGGCCGTGCC 
TACCGGCTGG GCATGTGTGA GCGCTGCTAC TGCTTCTGCA GGGACGCACA AGACCAACCG 
GACCCCTGCC AAGAAGGACT CCGGCCGGCG TCGGAAGTGC CACTGGCCGG TTTCCCGTCG 
GAAGAGCAGC AGGGCCAGAA CCGGCGGCGC CACCAGTCGG AGGCGGACGG GCGGAGCTCC 
CTCTCGGGGG CCGGAGCGTT TGCGTCACGC ATGCTCTCCG GCACCAAGGA CGACCTCAGC 
CTTCGGCAGG TGCCCCCAGA CAAGCGGGCC GGCATGGGGG GCGACAGCGA TCCGGGTGAC 
GGCAGGGGCG GTGGCATCCG GTTGGGCGGA GCGCATGGGT CTAGTCACCT GTCCGGAAGC 
ACTTCTGGAG AAGTGCTAAG TCACGTGTGC AGCTGCTGGT GCTGCGGCTG GGCCGAGGTG 
TACGTCCGGC GCCCCACGGG TAACACCTCC TGGGTCATGC GGCTCCAAAA TGACATGTTC 
TCCAAGACCT GCACACCCGA CTTCCCCCTG CCTGATGTGT CGGCCATCTT TCGCCAGCCC 
CAGATGATGG CCCAGGACTG GGCGGGACCC CCGGAAGAGC CTGTCGTCTG CAAACGGGAC 
CGACGTCCGA GCGAAGGAGG GCGAGTCAGA AGCGCCGGCA GCTCGGAGGA CATCTCTCCG 
AGTGCCCCTT TGGAGCGCAG CGGATCTCCC CTGCGCCGCA CAAACTCTAG TCCCGAGGTT 
CCCGCCCAGA GGCCCCCCGG AGACGGCTGC AAAACGGCCA GCCCCGGGTC GGCTCCGCAG 
AGTCCGTCGG CTATCTTCGA TCGGCTGGAC GAAGAGGGCG CCAGGCTGGC ACTGCGGAAG 
TCGCCGCGCA AGGACGACAG GTACGACTGC ATTCCCGAAG AATCGCACTG GGGTCTGCGG 
GCCCCTCCGT TCTCAAAGGC AAGGCTCAAA TTGGACCTGT CCCCGTCGGC CATGAGCGAG 
CCCCCAGTGG GGCCGGCCAC GTCGCTGCAC AGTCCCCCGC CACTGTCGCG TCTGCAAAGC 
CTGCAGGGTC GGCCCAACGA CGGGAGCAGC CCCACCCCGG CGAGCTCGTA CCGGGACCGG 
GGACACACGG TGTCCGGCAT CAGCCCCGTG ACGCACCAGG CCGCCACACC CACGGTGTCC 
TACATCGACA TGTACCGGTG CGGCATGAAC CCACGGTTCG TGTTCCTGCA CCTGTACCAC 
AACTCGTTCT TTGGGAAGCC GGGCACTGAC GAACCGATCC CTGTGCCGGA GTCAAAGTGG 
GATCGCAGCT CCAATTCCGG GAACGCGCTG GATTTTGTGC CGACCTACGA AACTCACAAG 
ATTGGCGTGG TGTACGTCGG CCCAGGACAG GCCCAGTCGG AGACGGCGAT GCTCTCGAAC 
CGGAGCGGCT CGGAGCGCTA CACTCGATTC CTGCTTGGCC TCGGACGCAC GGTGTGCTTG 
CGGGACCTGG ACACCAAGGC CACCTACACG GGAGGGCTGG ACGTGCGCGG CGCGGACGGC 
AAGTACTCCA TCAGCTGGCA GGACGACATC ATGCAGGTGA TCTTCCACGT GGCCACGCTG 
ATGCCTAACA AGGAGAACGA CCGGCAACGC AGCGACAAGA AGCGTCACAT CGGCAATGAC 
TACGTCATGG TGCTCTACAA CGACAGCTCC GAGTCCTGCG ACAGGGAAAC TTTCCGGGGC 
GAATTTCACC ATGCCTTCGT GATCATAGAA CCGTGGAAGC TGGGCAACAA TTTGGTGACT 
CTCGAGGGAA AAGCAGAGGT GATGGAGCTC TTTCGACACA GCGAGCCGCA GGTGGTTCCG 
GACAGCAGCT TGTCCCTGTA CGTTCGGCAA CTCGCATTGC ACGCCAACAT TGCGTGCAAC 
ATAGTGCTGC AAAAGACGAA GGGACCCGAC CTGTTTGTGA ACAACTGGCA CGGGCGGCTG 
CGCCACATCA ACCTCATCCG TCAGCGCCTC CTGGATGCCA GGGCCAAGGG GGAGTCTACA 
GACCCACCTG CTGCCACGGA GGACTTCACT CGGTACCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8039200 ( XM_029982219.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029838079.1 Ixodes scapularis tuberin (LOC8039200), transcript variant X2, mRNA.

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CloneID	OIa24905
Clone ID Related Accession (Same CDS sequence)	XM_029982219.1
Accession Version	XM_029982219.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	tuberin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021919882.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAA CAAGTCGCGA GAAAGAAAAG CCGCTGGAGC GCCTCAAGTC CCTCTTCGGC 
CTGGGCAAAG GCGGATCCTC CTCGGGGCCG GGTTTCGCCG TCCCCAAAAT CGAGGAGTTC 
GCCATCACGC CGGAGATCGT CCGGGATATC GGAAATGAGA GCAGCGTCTC GCACCGCACG 
AAATCCATCA AGGAACTCGT GGACATTGTA CGAACCAAAA GGCTCCAAGA GGGAGTTCCG 
GAGACCCTGT ACACGCAGAC CTGCGACCTG CTGCACCCCG CGCACGGGCA GGACGTGCGG 
CACACTGTAC TCTACTTCTA CTGCTGCCTC ATTCAGGGAC AGTTTGAGCG CCTGGACATC 
TCGCGGACGC ACTTCTTCAA CCTGATCCAG GACTTGCGAC ACCCAGAGGA CCTCGCTCTG 
AGACTGGACC TGCTGAAAGT GCTCACCGAC AAGGGAAAGT GTCTAAGCCA CTTCGAAGAC 
AAAGCACCCC AGTTCCTGCT TCAATGGATG CCAGAGGTGT TGAGTGCCGG CAGGGCGTTC 
GACTTCCTGT CTCTCCTGAT CAACGTCATC AAGTTCAACT CCTCCTTCCT GGACAAAGAA 
GTGGTTGCCG GATTTGTGCA AAATACCTGC ATCCTTTGCT GTAGGACCAA CTCTGAAAGC 
GACGTCAGGC TGGGACTGGA GGTGCTGGAC AGCGTAGTGG GCTACAGCCT ACTCTCATCC 
GAGGTGCTAC CTCACCTGGT GCCTACCCTG TGCCGGACGG TGGTCCTTGC CAAGCTGTCC 
GAGCCCAGCT GGAGGACGAT GCGCAACCTC CAGGGGACTC ATCTTGGCCA CAACGTCATC 
TCCTACCTCT GCCGGATCCT CGAAGACAGG GCCAACTACA GAGACTACAC CCTGCTCAAA 
GGAGCGGTGT TCTTCGTGGG TGCAGCGCTG TGGAGCCACA AGGCGGTGCC GACGCTCAAA 
CACACGCCTG CCGCGGTGCT GCCTTCCATA TACAGGGCCC TGGACTGCAA GCACCCCTCT 
GTGGCCTACG AGGCGGCCGT GGCTCTTCAG TGGCTGATCG GCGATCCGCC CAAGCCGATC 
ACTCCCTTCG TCTGGAAGTT TGTCCTTGAT GTCCTCGAGA AACTGGTTTG CCTCCCCGAG 
GTGGAGCAGG CTTCTGCCAG CTCACAGCAG TTGCAGCAGA TGGTTCATGA CATTGTGTCC 
AAGGTGGAAA AATCGTACGA CGAAGGGACG TACAAGGGCT GCCCCCAGCG CCTCTTCAAC 
ATTGTGGCAA AGTGCGCCTC CCTCAGGCCC GAGGCTTCCC TGCAGCGTCT GATCCAGTAC 
TGTGCGGAGA AGGCGCAGGT CCCCACCCTG AACTGGGTGA CCAACCTGTC GGAGATCCTG 
GAGAAGTACT TCAAGGCGGA GCCGCGGCCT TCTGTCCGCC TCTACACGCT CAACGTCGTC 
TCTTCCGTGC TCAGCAACAA CAGGCACCTC TACGAGGAGG AACTGATGAA GAAGGTGCTG 
CTCCCCCACC TCGGCGCGCT GGAAACAGAT CCAGATCCGG CCGTGGTGAC CGCGGCGGTC 
CAGCTGTTGA CCGACTTCTT TCAGGACAGC GGCAGCCAGC AGAACAATGA AGTTCTGGAC 
ATCATCGAAA AGGTGCTGAA CCACCCGACT TGGTCGACAA AGCCGAACGG CGCTGAGGAG 
GTGCTTCCCG TGGAGACGGC GGCCAGAGGT CTCATTGACG TTTTTCGGAA GAAGATGTGG 
CTGAGCCCTC AGTGTGCCGT GGCCACTTTC CGTCTGCTAG TTGACCACGT ACGTCGTCAA 
TACCGGCAGG ACCCCTGTCA GCTGCTGGCG CCCGTACGCC TAGTGATCAT GAAGTTCCTC 
GTGAGCCTGC GGACCAATGC CCTGTACCAG ATCGGCATGG ACGAAGGGTC GGAAGAGGGA 
ATTCGCTACA GCCCCCACCT CCTCTGCCCC TTCAGGGAAG GCTCAGCCAT GAAGCAGTGC 
TCCCCAGCAA GTGCCATTAC AAACAGCGGC GTGGTGGCTG CATCGGCACA ACCCCACATT 
GCTCACTTGG GTGTGGCGGA AGCCTTTGAC ACCATCATCG CAAGCATCAG GGAGGAGAGC 
GACTGGCCAG TCCTGTCGAC AGTCCTGCAG GGTCTTCCCG GCCTGCTAAA AGACAAAGCG 
CTTGTCCTGA GTGGGCAGAT GTCCTTCCGT GCACTCTGCG TCCGCCTCCG CAATCAAGCC 
AACGAGCGAA ACCGGAAGCC GCTGGACCAG ATGCACAGGG TGCCGGCCGG TGTCACGGCC 
TCTGACCTGA ACGCTCTGCT GTACCCAGCC ATGGGTGCCA TGACCATGTA CCGGACGGAA 
CTGGACAGCT CCTCCATGTG TATGGTCATC CACATGCTGG AGCACGGGCT GGCTTCCAAG 
CAGGCCTGCG TGTGCTTGCG TGCCTTGGTG CTGGCAACAC TGGAGATGAG GGACGCGATG 
GGCAAGTTGC TGCCCGAAGT GCTGCAGCGG CTCGCGCAGG TGTCGGCAAC ACTGGCGGTG 
GCCGTCCCGG TCCTCGAGTT CCTCTCAAAC CTCATCAGGA TCCCGCAGCT GTACGTCAGC 
TTTGTCGAGG AGCAGTACCG CAAGATCTTT GCCATTGCTC TGCCATACAC CAACCCCGTC 
AAGTTCAACC GCTTTGTGGT GGCGCTGGCT CATCACGTGA TTGCCATGTG GTTCCTCAAG 
TGCAGAATCA CGTTTCGGAA GGACCTTGTG CCTTACGTCA TCAAGAACCT CAAGAACAAC 
TTGGAACTCC ACGTGGAAGA CGGTGGCATG GAAGACACAA CGCAGCGGAA GAGAAGCAGC 
AGCCTCAACG AACCTATGGC GGTGGCAGCC GCGTCGCGCA GCGACATGGT GAAGCTACAG 
GAGGAGCTGC TCGAGACCTT CGTCGACCTC ATGTCTCAGT ACTGCTTTGG CACCTGCTCC 
TCGCAGCCGC ACAGGTCGGC CACGTCCGAG TTCATGCTGA AGCAGGGTCG CTCCATGACC 
TGGCTGCTGG GCCACAAGAT GGTGACCATC ACGACGAGTG GCTGCGGGGG CCGTGCCTAC 
CGGCTGGGCA TGTGTGAGCG CTGCTACTGC TTCTGCAGGG ACGCACAAGA CCAACCGGAC 
CCCTGCCAAG AAGGACTCCG GCCGGCGTCG GAAGTGCCAC TGGCCGGTTT CCCGTCGGAA 
GAGCAGCAGG GCCAGAACCG GCGGCGCCAC CAGTCGGAGG CGGACGGGCG GAGCTCCCTC 
TCGGGGGCCG GAGCGTTTGC GTCACGCATG CTCTCCGGCA CCAAGGACGA CCTCAGCCTT 
CGGCAGGTGC CCCCAGACAA GCGGGCCGGC ATGGGGGGCG ACAGCGATCC GGGTGACGGC 
AGGGGCGGTG GCATCCGGTT GGGCGGAGCG CATGGGTCTA GTCACCTGTC CGGAAGCACT 
TCTGGAGAAG TGCTAAGTCA CGTGTGCAGC TGCTGGTGCT GCGGCTGGGC CGAGGTGTAC 
GTCCGGCGCC CCACGGGTAA CACCTCCTGG GTCATGCGGC TCCAAAATGA CATGTTCTCC 
AAGACCTGCA CACCCGACTT CCCCCTGCCT GATGTGTCGG CCATCTTTCG CCAGCCCCAG 
ATGATGGCCC AGGACTGGGC GGGACCCCCG GAAGAGCCTG TCGTCTGCAA ACGGGACCGA 
CGTCCGAGCG AAGGAGGGCG AGTCAGAAGC GCCGGCAGCT CGGAGGACAT CTCTCCGAGT 
GCCCCTTTGG AGCGCAGCGG ATCTCCCCTG CGCCGCACAA ACTCTAGTCC CGAGGTTCCC 
GCCCAGAGGC CCCCCGGAGA CGGCTGCAAA ACGGCCAGCC CCGGGTCGGC TCCGCAGAGT 
CCGTCGGCTA TCTTCGATCG GCTGGACGAA GAGGGCGCCA GGCTGGCACT GCGGAAGTCG 
CCGCGCAAGG ACGACAGGTA CGACTGCATT CCCGAAGAAT CGCACTGGGG TCTGCGGGCC 
CCTCCGTTCT CAAAGGCAAG GCTCAAATTG GACCTGTCCC CGTCGGCCAT GAGCGAGCCC 
CCAGTGGGGC CGGCCACGTC GCTGCACAGT CCCCCGCCAC TGTCGCGTCT GCAAAGCCTG 
CAGGGTCGGC CCAACGACGG GAGCAGCCCC ACCCCGGCGA GCTCGTACCG GGACCGGGGA 
CACACGGTGT CCGGCATCAG CCCCGTGACG CACCAGGCCG CCACACCCAC GGTGTCCTAC 
ATCGACATGT ACCGGTGCGG CATGAACCCA CGGTTCGTGT TCCTGCACCT GTACCACAAC 
TCGTTCTTTG GGAAGCCGGG CACTGACGAA CCGATCCCTG TGCCGGAGTC AAAGTGGGAT 
CGCAGCTCCA ATTCCGGGAA CGCGCTGGAT TTTGTGCCGA CCTACGAAAC TCACAAGATT 
GGCGTGGTGT ACGTCGGCCC AGGACAGGCC CAGTCGGAGA CGGCGATGCT CTCGAACCGG 
AGCGGCTCGG AGCGCTACAC TCGATTCCTG CTTGGCCTCG GACGCACGGT GTGCTTGCGG 
GACCTGGACA CCAAGGCCAC CTACACGGGA GGGCTGGACG TGCGCGGCGC GGACGGCAAG 
TACTCCATCA GCTGGCAGGA CGACATCATG CAGGTGATCT TCCACGTGGC CACGCTGATG 
CCTAACAAGG AGAACGACCG GCAACGCAGC GACAAGAAGC GTCACATCGG CAATGACTAC 
GTCATGGTGC TCTACAACGA CAGCTCCGAG TCCTGCGACA GGGAAACTTT CCGGGGCGAA 
TTTCACCATG CCTTCGTGAT CATAGAACCG TGGAAGCTGG GCAACAATTT GGTGACTCTC 
GAGGGAAAAG CAGAGGTGAT GGAGCTCTTT CGACACAGCG AGCCGCAGGT GGTTCCGGAC 
AGCAGCTTGT CCCTGTACGT TCGGCAACTC GCATTGCACG CCAACATTGC GTGCAACATA 
GTGCTGCAAA AGACGAAGGG ACCCGACCTG TTTGTGAACA ACTGGCACGG GCGGCTGCGC 
CACATCAACC TCATCCGTCA GCGCCTCCTG GATGCCAGGG CCAAGGGGGA GTCTACAGAC 
CCACCTGCTG CCACGGAGGA CTTCACTCGG TACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029838079.1
CDS	116..5194
Translation	MSKTSREKEKPLERLKSLFGLGKGGSSSGPGFAVPKIEEFAITPEIVRDIGNESSVSHRTKSIKELVDIVRTKRLQEGVPETLYTQTCDLLHPAHGQDVRHTVLYFYCCLIQGQFERLDISRTHFFNLIQDLRHPEDLALRLDLLKVLTDKGKCLSHFEDKAPQFLLQWMPEVLSAGRAFDFLSLLINVIKFNSSFLDKEVVAGFVQNTCILCCRTNSESDVRLGLEVLDSVVGYSLLSSEVLPHLVPTLCRTVVLAKLSEPSWRTMRNLQGTHLGHNVISYLCRILEDRANYRDYTLLKGAVFFVGAALWSHKAVPTLKHTPAAVLPSIYRALDCKHPSVAYEAAVALQWLIGDPPKPITPFVWKFVLDVLEKLVCLPEVEQASASSQQLQQMVHDIVSKVEKSYDEGTYKGCPQRLFNIVAKCASLRPEASLQRLIQYCAEKAQVPTLNWVTNLSEILEKYFKAEPRPSVRLYTLNVVSSVLSNNRHLYEEELMKKVLLPHLGALETDPDPAVVTAAVQLLTDFFQDSGSQQNNEVLDIIEKVLNHPTWSTKPNGAEEVLPVETAARGLIDVFRKKMWLSPQCAVATFRLLVDHVRRQYRQDPCQLLAPVRLVIMKFLVSLRTNALYQIGMDEGSEEGIRYSPHLLCPFREGSAMKQCSPASAITNSGVVAASAQPHIAHLGVAEAFDTIIASIREESDWPVLSTVLQGLPGLLKDKALVLSGQMSFRALCVRLRNQANERNRKPLDQMHRVPAGVTASDLNALLYPAMGAMTMYRTELDSSSMCMVIHMLEHGLASKQACVCLRALVLATLEMRDAMGKLLPEVLQRLAQVSATLAVAVPVLEFLSNLIRIPQLYVSFVEEQYRKIFAIALPYTNPVKFNRFVVALAHHVIAMWFLKCRITFRKDLVPYVIKNLKNNLELHVEDGGMEDTTQRKRSSSLNEPMAVAAASRSDMVKLQEELLETFVDLMSQYCFGTCSSQPHRSATSEFMLKQGRSMTWLLGHKMVTITTSGCGGRAYRLGMCERCYCFCRDAQDQPDPCQEGLRPASEVPLAGFPSEEQQGQNRRRHQSEADGRSSLSGAGAFASRMLSGTKDDLSLRQVPPDKRAGMGGDSDPGDGRGGGIRLGGAHGSSHLSGSTSGEVLSHVCSCWCCGWAEVYVRRPTGNTSWVMRLQNDMFSKTCTPDFPLPDVSAIFRQPQMMAQDWAGPPEEPVVCKRDRRPSEGGRVRSAGSSEDISPSAPLERSGSPLRRTNSSPEVPAQRPPGDGCKTASPGSAPQSPSAIFDRLDEEGARLALRKSPRKDDRYDCIPEESHWGLRAPPFSKARLKLDLSPSAMSEPPVGPATSLHSPPPLSRLQSLQGRPNDGSSPTPASSYRDRGHTVSGISPVTHQAATPTVSYIDMYRCGMNPRFVFLHLYHNSFFGKPGTDEPIPVPESKWDRSSNSGNALDFVPTYETHKIGVVYVGPGQAQSETAMLSNRSGSERYTRFLLGLGRTVCLRDLDTKATYTGGLDVRGADGKYSISWQDDIMQVIFHVATLMPNKENDRQRSDKKRHIGNDYVMVLYNDSSESCDRETFRGEFHHAFVIIEPWKLGNNLVTLEGKAEVMELFRHSEPQVVPDSSLSLYVRQLALHANIACNIVLQKTKGPDLFVNNWHGRLRHINLIRQRLLDARAKGESTDPPAATEDFTRYL

Target ORF information:

RefSeq Version	XM_029982219.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis tuberin (LOC8039200), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029982219.1

ORF Insert Sequence:

ATGTCGAAAA CAAGTCGCGA GAAAGAAAAG CCGCTGGAGC GCCTCAAGTC CCTCTTCGGC 
CTGGGCAAAG GCGGATCCTC CTCGGGGCCG GGTTTCGCCG TCCCCAAAAT CGAGGAGTTC 
GCCATCACGC CGGAGATCGT CCGGGATATC GGAAATGAGA GCAGCGTCTC GCACCGCACG 
AAATCCATCA AGGAACTCGT GGACATTGTA CGAACCAAAA GGCTCCAAGA GGGAGTTCCG 
GAGACCCTGT ACACGCAGAC CTGCGACCTG CTGCACCCCG CGCACGGGCA GGACGTGCGG 
CACACTGTAC TCTACTTCTA CTGCTGCCTC ATTCAGGGAC AGTTTGAGCG CCTGGACATC 
TCGCGGACGC ACTTCTTCAA CCTGATCCAG GACTTGCGAC ACCCAGAGGA CCTCGCTCTG 
AGACTGGACC TGCTGAAAGT GCTCACCGAC AAGGGAAAGT GTCTAAGCCA CTTCGAAGAC 
AAAGCACCCC AGTTCCTGCT TCAATGGATG CCAGAGGTGT TGAGTGCCGG CAGGGCGTTC 
GACTTCCTGT CTCTCCTGAT CAACGTCATC AAGTTCAACT CCTCCTTCCT GGACAAAGAA 
GTGGTTGCCG GATTTGTGCA AAATACCTGC ATCCTTTGCT GTAGGACCAA CTCTGAAAGC 
GACGTCAGGC TGGGACTGGA GGTGCTGGAC AGCGTAGTGG GCTACAGCCT ACTCTCATCC 
GAGGTGCTAC CTCACCTGGT GCCTACCCTG TGCCGGACGG TGGTCCTTGC CAAGCTGTCC 
GAGCCCAGCT GGAGGACGAT GCGCAACCTC CAGGGGACTC ATCTTGGCCA CAACGTCATC 
TCCTACCTCT GCCGGATCCT CGAAGACAGG GCCAACTACA GAGACTACAC CCTGCTCAAA 
GGAGCGGTGT TCTTCGTGGG TGCAGCGCTG TGGAGCCACA AGGCGGTGCC GACGCTCAAA 
CACACGCCTG CCGCGGTGCT GCCTTCCATA TACAGGGCCC TGGACTGCAA GCACCCCTCT 
GTGGCCTACG AGGCGGCCGT GGCTCTTCAG TGGCTGATCG GCGATCCGCC CAAGCCGATC 
ACTCCCTTCG TCTGGAAGTT TGTCCTTGAT GTCCTCGAGA AACTGGTTTG CCTCCCCGAG 
GTGGAGCAGG CTTCTGCCAG CTCACAGCAG TTGCAGCAGA TGGTTCATGA CATTGTGTCC 
AAGGTGGAAA AATCGTACGA CGAAGGGACG TACAAGGGCT GCCCCCAGCG CCTCTTCAAC 
ATTGTGGCAA AGTGCGCCTC CCTCAGGCCC GAGGCTTCCC TGCAGCGTCT GATCCAGTAC 
TGTGCGGAGA AGGCGCAGGT CCCCACCCTG AACTGGGTGA CCAACCTGTC GGAGATCCTG 
GAGAAGTACT TCAAGGCGGA GCCGCGGCCT TCTGTCCGCC TCTACACGCT CAACGTCGTC 
TCTTCCGTGC TCAGCAACAA CAGGCACCTC TACGAGGAGG AACTGATGAA GAAGGTGCTG 
CTCCCCCACC TCGGCGCGCT GGAAACAGAT CCAGATCCGG CCGTGGTGAC CGCGGCGGTC 
CAGCTGTTGA CCGACTTCTT TCAGGACAGC GGCAGCCAGC AGAACAATGA AGTTCTGGAC 
ATCATCGAAA AGGTGCTGAA CCACCCGACT TGGTCGACAA AGCCGAACGG CGCTGAGGAG 
GTGCTTCCCG TGGAGACGGC GGCCAGAGGT CTCATTGACG TTTTTCGGAA GAAGATGTGG 
CTGAGCCCTC AGTGTGCCGT GGCCACTTTC CGTCTGCTAG TTGACCACGT ACGTCGTCAA 
TACCGGCAGG ACCCCTGTCA GCTGCTGGCG CCCGTACGCC TAGTGATCAT GAAGTTCCTC 
GTGAGCCTGC GGACCAATGC CCTGTACCAG ATCGGCATGG ACGAAGGGTC GGAAGAGGGA 
ATTCGCTACA GCCCCCACCT CCTCTGCCCC TTCAGGGAAG GCTCAGCCAT GAAGCAGTGC 
TCCCCAGCAA GTGCCATTAC AAACAGCGGC GTGGTGGCTG CATCGGCACA ACCCCACATT 
GCTCACTTGG GTGTGGCGGA AGCCTTTGAC ACCATCATCG CAAGCATCAG GGAGGAGAGC 
GACTGGCCAG TCCTGTCGAC AGTCCTGCAG GGTCTTCCCG GCCTGCTAAA AGACAAAGCG 
CTTGTCCTGA GTGGGCAGAT GTCCTTCCGT GCACTCTGCG TCCGCCTCCG CAATCAAGCC 
AACGAGCGAA ACCGGAAGCC GCTGGACCAG ATGCACAGGG TGCCGGCCGG TGTCACGGCC 
TCTGACCTGA ACGCTCTGCT GTACCCAGCC ATGGGTGCCA TGACCATGTA CCGGACGGAA 
CTGGACAGCT CCTCCATGTG TATGGTCATC CACATGCTGG AGCACGGGCT GGCTTCCAAG 
CAGGCCTGCG TGTGCTTGCG TGCCTTGGTG CTGGCAACAC TGGAGATGAG GGACGCGATG 
GGCAAGTTGC TGCCCGAAGT GCTGCAGCGG CTCGCGCAGG TGTCGGCAAC ACTGGCGGTG 
GCCGTCCCGG TCCTCGAGTT CCTCTCAAAC CTCATCAGGA TCCCGCAGCT GTACGTCAGC 
TTTGTCGAGG AGCAGTACCG CAAGATCTTT GCCATTGCTC TGCCATACAC CAACCCCGTC 
AAGTTCAACC GCTTTGTGGT GGCGCTGGCT CATCACGTGA TTGCCATGTG GTTCCTCAAG 
TGCAGAATCA CGTTTCGGAA GGACCTTGTG CCTTACGTCA TCAAGAACCT CAAGAACAAC 
TTGGAACTCC ACGTGGAAGA CGGTGGCATG GAAGACACAA CGCAGCGGAA GAGAAGCAGC 
AGCCTCAACG AACCTATGGC GGTGGCAGCC GCGTCGCGCA GCGACATGGT GAAGCTACAG 
GAGGAGCTGC TCGAGACCTT CGTCGACCTC ATGTCTCAGT ACTGCTTTGG CACCTGCTCC 
TCGCAGCCGC ACAGGTCGGC CACGTCCGAG TTCATGCTGA AGCAGGGTCG CTCCATGACC 
TGGCTGCTGG GCCACAAGAT GGTGACCATC ACGACGAGTG GCTGCGGGGG CCGTGCCTAC 
CGGCTGGGCA TGTGTGAGCG CTGCTACTGC TTCTGCAGGG ACGCACAAGA CCAACCGGAC 
CCCTGCCAAG AAGGACTCCG GCCGGCGTCG GAAGTGCCAC TGGCCGGTTT CCCGTCGGAA 
GAGCAGCAGG GCCAGAACCG GCGGCGCCAC CAGTCGGAGG CGGACGGGCG GAGCTCCCTC 
TCGGGGGCCG GAGCGTTTGC GTCACGCATG CTCTCCGGCA CCAAGGACGA CCTCAGCCTT 
CGGCAGGTGC CCCCAGACAA GCGGGCCGGC ATGGGGGGCG ACAGCGATCC GGGTGACGGC 
AGGGGCGGTG GCATCCGGTT GGGCGGAGCG CATGGGTCTA GTCACCTGTC CGGAAGCACT 
TCTGGAGAAG TGCTAAGTCA CGTGTGCAGC TGCTGGTGCT GCGGCTGGGC CGAGGTGTAC 
GTCCGGCGCC CCACGGGTAA CACCTCCTGG GTCATGCGGC TCCAAAATGA CATGTTCTCC 
AAGACCTGCA CACCCGACTT CCCCCTGCCT GATGTGTCGG CCATCTTTCG CCAGCCCCAG 
ATGATGGCCC AGGACTGGGC GGGACCCCCG GAAGAGCCTG TCGTCTGCAA ACGGGACCGA 
CGTCCGAGCG AAGGAGGGCG AGTCAGAAGC GCCGGCAGCT CGGAGGACAT CTCTCCGAGT 
GCCCCTTTGG AGCGCAGCGG ATCTCCCCTG CGCCGCACAA ACTCTAGTCC CGAGGTTCCC 
GCCCAGAGGC CCCCCGGAGA CGGCTGCAAA ACGGCCAGCC CCGGGTCGGC TCCGCAGAGT 
CCGTCGGCTA TCTTCGATCG GCTGGACGAA GAGGGCGCCA GGCTGGCACT GCGGAAGTCG 
CCGCGCAAGG ACGACAGGTA CGACTGCATT CCCGAAGAAT CGCACTGGGG TCTGCGGGCC 
CCTCCGTTCT CAAAGGCAAG GCTCAAATTG GACCTGTCCC CGTCGGCCAT GAGCGAGCCC 
CCAGTGGGGC CGGCCACGTC GCTGCACAGT CCCCCGCCAC TGTCGCGTCT GCAAAGCCTG 
CAGGGTCGGC CCAACGACGG GAGCAGCCCC ACCCCGGCGA GCTCGTACCG GGACCGGGGA 
CACACGGTGT CCGGCATCAG CCCCGTGACG CACCAGGCCG CCACACCCAC GGTGTCCTAC 
ATCGACATGT ACCGGTGCGG CATGAACCCA CGGTTCGTGT TCCTGCACCT GTACCACAAC 
TCGTTCTTTG GGAAGCCGGG CACTGACGAA CCGATCCCTG TGCCGGAGTC AAAGTGGGAT 
CGCAGCTCCA ATTCCGGGAA CGCGCTGGAT TTTGTGCCGA CCTACGAAAC TCACAAGATT 
GGCGTGGTGT ACGTCGGCCC AGGACAGGCC CAGTCGGAGA CGGCGATGCT CTCGAACCGG 
AGCGGCTCGG AGCGCTACAC TCGATTCCTG CTTGGCCTCG GACGCACGGT GTGCTTGCGG 
GACCTGGACA CCAAGGCCAC CTACACGGGA GGGCTGGACG TGCGCGGCGC GGACGGCAAG 
TACTCCATCA GCTGGCAGGA CGACATCATG CAGGTGATCT TCCACGTGGC CACGCTGATG 
CCTAACAAGG AGAACGACCG GCAACGCAGC GACAAGAAGC GTCACATCGG CAATGACTAC 
GTCATGGTGC TCTACAACGA CAGCTCCGAG TCCTGCGACA GGGAAACTTT CCGGGGCGAA 
TTTCACCATG CCTTCGTGAT CATAGAACCG TGGAAGCTGG GCAACAATTT GGTGACTCTC 
GAGGGAAAAG CAGAGGTGAT GGAGCTCTTT CGACACAGCG AGCCGCAGGT GGTTCCGGAC 
AGCAGCTTGT CCCTGTACGT TCGGCAACTC GCATTGCACG CCAACATTGC GTGCAACATA 
GTGCTGCAAA AGACGAAGGG ACCCGACCTG TTTGTGAACA ACTGGCACGG GCGGCTGCGC 
CACATCAACC TCATCCGTCA GCGCCTCCTG GATGCCAGGG CCAAGGGGGA GTCTACAGAC 
CCACCTGCTG CCACGGAGGA CTTCACTCGG TACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC8039200 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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