LINJ_08_0080 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	LINJ_08_0080
Entrez Gene ID	5066753
Full Name	putative ATP-dependent DEAD/H RNA helicase
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_001463312.1	XP_001463349.1	putative ATP-dependent DEAD/H RNA helicase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LINJ_08_0080 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LINJ_08_0080 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc00727	XM_001463312.1 Latest version!	Leishmania infantum JPCM5 putative ATP-dependent DEAD/H RNA helicase partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LINJ_08_0080 ( XM_001463312.1 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_001463349.1 Leishmania infantum JPCM5 putative ATP-dependent DEAD/H RNA helicase partial mRNA.

Price & Availability↑

CloneID	OLc00727
Clone ID Related Accession (Same CDS sequence)	XM_001463312.1
Accession Version	XM_001463312.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-07-15
Organism	Leishmania infantum JPCM5
Product	putative ATP-dependent DEAD/H RNA helicase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009392). COMPLETENESS: incomplete on both ends.

ATGAACTACG CCTCCATTCG CACAGACTTC TACGTGGTGC CGCCGGACAT GACGAATCTC 
ACAGCGCAGG AGATGCGAGA ACTGCTGCGA GAGCTGGATG GGGCAAAGGT TCACGGGAAA 
GATGTGCCGC GGCCGATTCG CTCCTGGCAT GGCACCGGAT TGCCGGATCG CGTGTTGGAG 
GTGCTGGAGG AGCACGAGTA CAAGTGCCCT TTTGCGGTGC AGTCGCTCGG CGTGCCGGCG 
CTGATGAGCG GACGGGATTT GCTCCTCACA GCAAAGACGG GGTCCGGCAA AACGCTCTGC 
TATGCGCTGC CGCTCATCCG GCACTGCGCG GACCAGCCGC GGTGTGAAAA GGGCGAGGGC 
CCTATAGGGC TGGTGCTTGT GCCCACGCAG GAGCTCGCTA TGCAGGTATT CACGCTTCTC 
GACGAGTTGG GGGAGGCTGC TGGGCTGCGC TGTGTCGCCT CGTATGGCAG CACGTCGCTA 
TCCGACAATA TTCGCCATGC CAAGGCGGGG TGTGAGTTGA TGGTGGCGAC GCCCGGCCGG 
CTGCTAGACC TTCTCACAGT GAATGGAGGG AAGACGTTGA GCCTGTCGCG CGTCTCCTTC 
GTCATTGTTG ACGAGGCGGA CCGCCTCTTC GACAGCGGCT TCATGGAGCA CGTGGAGGCC 
TTTCTGAAGA ACATACGCCC TGACCGCGTA ACAGGGATGA TCAGCGCTAC GATGCCAAAG 
GAGCTGCGCG GGGTAGTAGC GCAACACCTG CGGAACCCTG TTGTCATTTC CGTCGGCGGA 
AAACCGACGC CTGCCTCGAA CGTCGAGCAG CAGTTCTTCT TTTTCGACGA GGAGGTATAC 
GACGCGAACA ACATCAAGGC AGACATGAGC CCACGTCTTG TAAAGTTACT GGCATTGCTG 
GGAGACGAGG GCGGTGACGG TCAGAACCTA ATCCTGGTCT TTACTCAACG GAAGGAGGAG 
GTAGACGAGT TGCTGGGGCG GCTCACCACG CTGGGCTACG CCAACCGCGT GGCAACACTC 
TACAGCGGCA TGGACCCCAT CGACCGCGAG TTCGCGCTGG AGCACTTTGT TCCAGGCAAG 
CAGTTTATTC TCGTCGCCAC CGCAGTTGCA GAGCGCGGCT TGGATATTCC CTACCTCGGC 
CTTGTGATCA ACTACCGGTT GCCCAACCAC TACGAGGCGT ACGTGCATCG TATCGGCCGC 
ACCGGCCGCG CCGGGCGCAG CGGGCGTGCC GTGAGCTTTT TCACTCGTGG CAAGGATGAC 
GACATCGCTC CAGAGCTGGT GGAAGGGCTG GAGCGTGCGG AGCAGCGAGT ACCAGAGGAG 
TTGTACGAGG TTGCGGAGAA GGTGCGTGCG CTGCGCAAGT CTGGCGATGC AAGGTACAAC 
AGCGGCTTCT TTCGCGGCTA CCGCGACGCG AAGGCGCAGC GCTTCACTGA CCGTGACAAG 
AAGGAGAAGA TGCGCGAGGC TGCCCGCGCG GCCGGGTTAG AGCAGTTTCT CAGCTCCTCC 
TCCGAGTCCG ACTCGGATGC CTCGAGCGAC GAAGATGCAA ACATCACTGC GGTGCCGGTG 
CAATCGAGAG CTCGCGCCGC GTCGGCAGAA GAGGCAGCGG CGAGCTCGAT GGCGCTGACG 
CTGCACCGTG GTGCGCAAAG CGGCGCGTTG ACGTTGTCCG CTGCGCAGGA GGACCGCATT 
TCCAAGGCGC TGGCGTTCGC GCAGAAAACA ACCACGGCGG CAACGATGGC GGCGGACGAG 
GCGACGATGG TGCGCTTTCA GAGTGAGTAC CCCATCAATG ACTTGCCCGA TGCCGTGCGT 
GGCAAGCTGC AGAGCGGCGC TTTTATGCGC TCCATCGCGG AGGAGACGGA AACATCATTG 
ATCCGAAAGG GCGTGTATTT CGACCCACGC TACAAGCACT CGCGGCGGAT GAAGGAGGGG 
GAGAAGCCGC TGTATTTTCT GATAGTGGGA AAGTCGATGG AGGCGGTGCG GGCAGCTCGC 
AACAAACTCG ACGAGGTGAA GGCAGAGCTG CTGAGCAAAC AGAAGAAGAC GGGATCCGTG 
ACTGGTGCCA CGATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001463349.1
CDS	1..2058
Translation	MNYASIRTDFYVVPPDMTNLTAQEMRELLRELDGAKVHGKDVPRPIRSWHGTGLPDRVLEVLEEHEYKCPFAVQSLGVPALMSGRDLLLTAKTGSGKTLCYALPLIRHCADQPRCEKGEGPIGLVLVPTQELAMQVFTLLDELGEAAGLRCVASYGSTSLSDNIRHAKAGCELMVATPGRLLDLLTVNGGKTLSLSRVSFVIVDEADRLFDSGFMEHVEAFLKNIRPDRVTGMISATMPKELRGVVAQHLRNPVVISVGGKPTPASNVEQQFFFFDEEVYDANNIKADMSPRLVKLLALLGDEGGDGQNLILVFTQRKEEVDELLGRLTTLGYANRVATLYSGMDPIDREFALEHFVPGKQFILVATAVAERGLDIPYLGLVINYRLPNHYEAYVHRIGRTGRAGRSGRAVSFFTRGKDDDIAPELVEGLERAEQRVPEELYEVAEKVRALRKSGDARYNSGFFRGYRDAKAQRFTDRDKKEKMREAARAAGLEQFLSSSSESDSDASSDEDANITAVPVQSRARAASAEEAAASSMALTLHRGAQSGALTLSAAQEDRISKALAFAQKTTTAATMAADEATMVRFQSEYPINDLPDAVRGKLQSGAFMRSIAEETETSLIRKGVYFDPRYKHSRRMKEGEKPLYFLIVGKSMEAVRAARNKLDEVKAELLSKQKKTGSVTGATI

Target ORF information:

RefSeq Version	XM_001463312.1
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 putative ATP-dependent DEAD/H RNA helicase partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001463312.1

ORF Insert Sequence:

ATGAACTACG CCTCCATTCG CACAGACTTC TACGTGGTGC CGCCGGACAT GACGAATCTC 
ACAGCGCAGG AGATGCGAGA ACTGCTGCGA GAGCTGGATG GGGCAAAGGT TCACGGGAAA 
GATGTGCCGC GGCCGATTCG CTCCTGGCAT GGCACCGGAT TGCCGGATCG CGTGTTGGAG 
GTGCTGGAGG AGCACGAGTA CAAGTGCCCT TTTGCGGTGC AGTCGCTCGG CGTGCCGGCG 
CTGATGAGCG GACGGGATTT GCTCCTCACA GCAAAGACGG GGTCCGGCAA AACGCTCTGC 
TATGCGCTGC CGCTCATCCG GCACTGCGCG GACCAGCCGC GGTGTGAAAA GGGCGAGGGC 
CCTATAGGGC TGGTGCTTGT GCCCACGCAG GAGCTCGCTA TGCAGGTATT CACGCTTCTC 
GACGAGTTGG GGGAGGCTGC TGGGCTGCGC TGTGTCGCCT CGTATGGCAG CACGTCGCTA 
TCCGACAATA TTCGCCATGC CAAGGCGGGG TGTGAGTTGA TGGTGGCGAC GCCCGGCCGG 
CTGCTAGACC TTCTCACAGT GAATGGAGGG AAGACGTTGA GCCTGTCGCG CGTCTCCTTC 
GTCATTGTTG ACGAGGCGGA CCGCCTCTTC GACAGCGGCT TCATGGAGCA CGTGGAGGCC 
TTTCTGAAGA ACATACGCCC TGACCGCGTA ACAGGGATGA TCAGCGCTAC GATGCCAAAG 
GAGCTGCGCG GGGTAGTAGC GCAACACCTG CGGAACCCTG TTGTCATTTC CGTCGGCGGA 
AAACCGACGC CTGCCTCGAA CGTCGAGCAG CAGTTCTTCT TTTTCGACGA GGAGGTATAC 
GACGCGAACA ACATCAAGGC AGACATGAGC CCACGTCTTG TAAAGTTACT GGCATTGCTG 
GGAGACGAGG GCGGTGACGG TCAGAACCTA ATCCTGGTCT TTACTCAACG GAAGGAGGAG 
GTAGACGAGT TGCTGGGGCG GCTCACCACG CTGGGCTACG CCAACCGCGT GGCAACACTC 
TACAGCGGCA TGGACCCCAT CGACCGCGAG TTCGCGCTGG AGCACTTTGT TCCAGGCAAG 
CAGTTTATTC TCGTCGCCAC CGCAGTTGCA GAGCGCGGCT TGGATATTCC CTACCTCGGC 
CTTGTGATCA ACTACCGGTT GCCCAACCAC TACGAGGCGT ACGTGCATCG TATCGGCCGC 
ACCGGCCGCG CCGGGCGCAG CGGGCGTGCC GTGAGCTTTT TCACTCGTGG CAAGGATGAC 
GACATCGCTC CAGAGCTGGT GGAAGGGCTG GAGCGTGCGG AGCAGCGAGT ACCAGAGGAG 
TTGTACGAGG TTGCGGAGAA GGTGCGTGCG CTGCGCAAGT CTGGCGATGC AAGGTACAAC 
AGCGGCTTCT TTCGCGGCTA CCGCGACGCG AAGGCGCAGC GCTTCACTGA CCGTGACAAG 
AAGGAGAAGA TGCGCGAGGC TGCCCGCGCG GCCGGGTTAG AGCAGTTTCT CAGCTCCTCC 
TCCGAGTCCG ACTCGGATGC CTCGAGCGAC GAAGATGCAA ACATCACTGC GGTGCCGGTG 
CAATCGAGAG CTCGCGCCGC GTCGGCAGAA GAGGCAGCGG CGAGCTCGAT GGCGCTGACG 
CTGCACCGTG GTGCGCAAAG CGGCGCGTTG ACGTTGTCCG CTGCGCAGGA GGACCGCATT 
TCCAAGGCGC TGGCGTTCGC GCAGAAAACA ACCACGGCGG CAACGATGGC GGCGGACGAG 
GCGACGATGG TGCGCTTTCA GAGTGAGTAC CCCATCAATG ACTTGCCCGA TGCCGTGCGT 
GGCAAGCTGC AGAGCGGCGC TTTTATGCGC TCCATCGCGG AGGAGACGGA AACATCATTG 
ATCCGAAAGG GCGTGTATTT CGACCCACGC TACAAGCACT CGCGGCGGAT GAAGGAGGGG 
GAGAAGCCGC TGTATTTTCT GATAGTGGGA AAGTCGATGG AGGCGGTGCG GGCAGCTCGC 
AACAAACTCG ACGAGGTGAA GGCAGAGCTG CTGAGCAAAC AGAAGAAGAC GGGATCCGTG 
ACTGGTGCCA CGATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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LINJ_08_0080 cDNA ORF clone, Leishmania infantum JPCM5

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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