POLIB cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	POLIB
Entrez Gene ID	5067416
Full Name	putative mitochondrial DNA polymerase I protein B
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_001463998.1	XP_001464035.1	putative mitochondrial DNA polymerase I protein B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following POLIB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLIB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc01236	XM_001463998.1 Latest version!	Leishmania infantum JPCM5 putative mitochondrial DNA polymerase I protein B (POLIB), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLIB ( XM_001463998.1 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_001464035.1 Leishmania infantum JPCM5 putative mitochondrial DNA polymerase I protein B (POLIB), partial mRNA.

Price & Availability↑

CloneID	OLc01236
Clone ID Related Accession (Same CDS sequence)	XM_001463998.1
Accession Version	XM_001463998.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-07-15
Organism	Leishmania infantum JPCM5
Product	putative mitochondrial DNA polymerase I protein B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009397). COMPLETENESS: incomplete on both ends.

ATGTTTCGTC GCTTGTCTAG CTCCTGGCCC CGCGCGCTGG TGCAACCACT GCGCTCAAGC 
TGCACTGTGC AGTCCCTGAC GAAGGCCCAG GCAGCGCTGG CAAGAGGGAA GGCTCCGTCA 
ATGGACGCCG CTGCGGAGTA CGCCGAGTTC TTTCGCGTCC CCATTTCCGT GGACGCCGGT 
GTCGTACGCA GCACGCAGGA ATACTACACC AACATCCGGA GGCTGGCAGC CGAGAATCAA 
AGCGCCGTTG CTCAGCGTAA CTCGCCGAAT AAGAGCTGCG GGGAAAGCAG CGTGGCGACT 
TTCCTGGTGG GGTACCACTT CCAGACAGCC ACCTTCCAGA TCTTTTCCAT CGCGGCGGAC 
ACCATTGTGT GTAGCGTGGA TACGAGGATG GAGGCTTCCG AGGTGTGGAG TGTAGCGGAT 
GAGCTCATGA CCCACATTAG CGGCGAATCA CCGCAGCTTG CTCTCATACC CGTTGTAGAG 
ACGGAACAGG AGCGGAAAAG GCTGCAGGAG CCTCTCCAAA AGCTCACGGC CTTGCTGAAG 
TTCAGCGGGC TGCAAGTGGC CACTCACGTT GACACCCTCG AGCTCAGCTC CGTGCTGTGC 
CACAGTCCTC CGTCGCCACA TGATATCACA CAGGACGCCT TGTCTGGTGT GTCTCCAGCT 
CCTATGACGT GCACGTGGTT CCAGGCGCAT TGGGCATTCA TCCGCACTGC AGCGTGCCAG 
GCGATGTCGG CAGACCCGTT GCAGACTGTG AGCACGTACG TGCTGCCCCG CTTCTCCGTC 
TACCTCATCC ATCTCGTGCG CGCCGGCACT CGTGGGCTTA ACGTGTCGCT CTGGGACCCG 
GTGAGCCGCC GCCGCTTCTC CGCCAAGGGC ATGCTCTCTG ACGTGCTTGC CTGCCTGCTC 
GATACGCGTT CCAGCCAGAT GATCTTGGTG CCCACGAACC GAACCGACCG CGCTGCACTC 
TTTCGCTGCC GCACCGCCGT CGTGAAGGCC GGCCACGATT GCGTCGTCGT ATACGCGTCA 
GAGGTGAGCG ACCGCCTTCG TGGCTGCTAC CAGTCCGACC TGATGCGGTA CTGGGGGGCG 
ATGAAGGAGG CTGCGAACTT TCTGGACGCG GATCTGCTCT TCGGCATCTA CACCGCAGCC 
GGCAACACAC TCCGCAGTTA TCAGCGCAAG AAGCTTTCCC AGAGCCAGAT GTGTGACCAC 
TTGCGTCTGC TAAACAGCTC CTCGGATGAG GGCATCGCGA CCCCACAGAA CGTCCTCGAC 
TGGGCCTGCT ACTTTACTCT CCCCAAGCGT GTTGCAGCTG AGAAGGCGGA GCTGCGCCAT 
TACGACAAGT TCATCACCAT CGCCTACGTC GCCACCGACC ACACCGTGCA TGCCCAGCCG 
ATGAACCCGG TGGCGGACAT CAACCACGTG CTGAGTGCGT GCATCACCGA CCACGCGAAC 
CGGACAGTAG AGCCGTGGGC CATCTACACG AAGCGCGGGC AGTTCTGCTT ACCCAGCCTC 
GATGGATACG ACGTGCTCGT CACACACGAT GCCAAGTCGC TTGTGCTGCT GCTGTCGGGG 
GATGCCGAGC TACAGAAGTT CATCAAGCGC GGCGGCCGTG TGTGGTGTGT GACGCTGGCC 
GAGTACCTTC TCGAGGCGCA GCGATGTACC ACTGGCAGCA ACGGCCTTTA CGATTTGGCC 
CTACGTCACG GTGTGCAGCT GCCACCATCG TCGCGCGTGG GTACGCCAAA CGACCGTCTC 
CCGTTTGCCT TTCAGCGGCA GTTTCTCCTC CATGCAGCGC CCGCGGTAGT GAAGGTGTTT 
GTGGAGCAGT TGAAGCGTGC GCTTGAACAA TGCCAGGTGC TGAGCTTGGC GCACCGCATG 
GACTCCCTGC TTGCGATGGC GAGCATCGAG AAGGCCGGCA TTCACATCGA TGCGGAGGAG 
GCGGCACGGC AGACGGCCTC GCTGAAGAGC GCCGCGTCTG TGCTGGACGC GGCGATGGAG 
GCGTACGTGC CGAGCGAGGT ACCGGTTGAC ATGAGGATTT ACTTTGACTG GGCGTCGCTG 
CAGCAGCAGC ACGCCTACTT CTTTGGTGGC ACAATTTCGC TAGGACACCA GGCCCACGCC 
CGCGATACCC CTCTATGGAC TTCGAATGTG GTCCATCTTT GCCACCGCTA CGGCGCTTTC 
CCACACATGG TTGGGGAGCT ACACCTGCAG CGTTACGCGG CCCAATGCGC TCTGCCCACG 
TCGGGAGGGC TGCCGAACCG CGTCCATCAG CACATCGAGG CGCAGGGCTC GCAGAAACTA 
AAAACGTACC GCATCGTCTT CTTTGACATC GAGACAACGG GGCTCAACCC AAGCACGGAC 
GCCATTGTGG AAGTGGCGAT GTTCGACCCG ATCGAGAACA GCACGTTTCA CACGCTCGTG 
AACCCGCAGC GTTCCATCAC TCCGCGAACT GTTGGGATTC ACCACATCAC AAACGCTATG 
GTGCGCGACG CGCCGACCGT GGACGTGGTC GCGAAGAGCA TTGGCCAGTA TCTGCGCCTC 
GACAAGGCCT CGTACAACCC GCATGAGATT TTGGTGCTGG TAGGCCACAA CGTCTTCTCC 
GTGGACGAGC CGATGCTGCG CCGCGCGCTC GAGTGTCACG CGCCGGAGTG CCAGCTGGAC 
GGCATTCTGT TTTGCGACTC ACTGGCGCTC CTTAATGCTC ACCGGAGCGA GCTGCGATGG 
CGCATGCGCA CCAAACGCAC GAACCAACCC CTCATGGATG CCCTCGCATC CTCTCTGCGC 
CTCAGTCGCC TGATCACAGC GCTCAACGTG CGGCCGGAAG GCGACCTGCA CCGCGCTGAC 
ACGGACACAA AGGCACTCTG GTATGTGCTG GTCAACATCC TCGGTGTCGC CGACAAAGAC 
GCTGTAGTGC AGCGGGACAA GATTTTGATC GAGGCCGCCA ACTGCTTCCT GCGCTCTCCG 
TCGATCGGGT GCTTTGTACC AGCAGAGCGT CAAAACCGGC TCGTGAAGGT GCGGCTGCCC 
GGCGTGGCTG CCGACTACGT CCGCAACGTG AAGCTGATTG CAAGTCTGCA GAGCAAAGTC 
TTCTCCGAAA CTGTGCTCGC GTCCTTGCAC GCGCACGGCG TGAAGCCGGC AGGCCTGCTC 
CTGCAGCGGC AGCTGCTCGA CCGTCACACC TCCACGTTCC TGCAACCGAG CACAGGCGGG 
CGGCTGGCCA TTCTGCACCG AGACGGCAGA GTGCACCAGC ACATCGACAT GACCGCCACG 
ACGACGTCGC GTACTGTTAG CGCCTACCCG AGTTGCCAGA ACATTCCGAA GGACGATAAG 
TCGTCTGTGC GGCGTCTCTT TGTGTCCCGC TTCGGCTCCA AGGGGCGTTG CGTCGAGGTG 
GACTACTCGC AGCTGGAGAT TGTGGTGCTG GCGATCCTGT GCAACGACGC AAACCTCACA 
AAAGACCTCA ATAGCGGTGT CGACTTCCAC GTGAAGCGGG CGGCGTTCTT TAGCGGGCTG 
CCGTACGACG AGATTTACCA GGGTTACAAG CGCAATGTCC CCAAGTACGT CAAGCTGCGC 
AAGACGGCGA AGCAGTTCTC CTTTCAACGT CTGTACGGCG CTGGGGTGCC GCTGTTGCAC 
AAGACCACCG GCATTCCCGT AAAGGACCTG GAGGCGTCGA TTCAGAGAGA GAATGAGGAG 
TACCCCGGCA TCGCGCAGTT TCACCGCATT ATCCGGTCGG TCGCGCTTCG CCCGAACAAC 
CCAGGACTGC CCACCAGCTT CATTGCCGAG ATGCCGACGG GGCTGCGGAT GAGCCTGCGC 
ACGCGGGATG TGGTGCTGAA CCTGCCTCCC ATCAAGAACT ACCCAATTCA AGGCTACGGC 
GCGGAGCTGG CGCAGATGAT GCTCGGCCGG CTCTACCGGC ACTTTGTGCG GAAAGACTTC 
TACGAGGACC GCGCGTTTCT CATCAACTTT GTTCACGACT CGGTGTGGAT GGACTGCCAC 
GTCGATGTGC TGCGGGAATG CGTAACGGAC ACATGCCGCA TCCTCGGCTC GGTGCACGAG 
TACGTCCCAA AGGTGTTCCC TGGAGTGAAG ATCAGCGTGC CGCTGCAAGT CTCTGCGTCG 
TGCGGGGTAG ACATGTGCTC CATGGAGAGC ATCAAAGACG ACGACTACAC GTTTGTGTCG 
AAGCAGCGCA AGACGAGGCC CGCAGAGGTG CAGGACTTTT TGGACCTGAC GACGGCCAAG 
TCGAACTTCT CGGCGGTGAT GGAGGAGAGC GTCGCGTCCG AGGAGGAGGA GAGCGGGGAA 
ACGGCGACGT CCAAGGCGGA GGCGACCGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001464035.1
CDS	1..4293
Translation	MFRRLSSSWPRALVQPLRSSCTVQSLTKAQAALARGKAPSMDAAAEYAEFFRVPISVDAGVVRSTQEYYTNIRRLAAENQSAVAQRNSPNKSCGESSVATFLVGYHFQTATFQIFSIAADTIVCSVDTRMEASEVWSVADELMTHISGESPQLALIPVVETEQERKRLQEPLQKLTALLKFSGLQVATHVDTLELSSVLCHSPPSPHDITQDALSGVSPAPMTCTWFQAHWAFIRTAACQAMSADPLQTVSTYVLPRFSVYLIHLVRAGTRGLNVSLWDPVSRRRFSAKGMLSDVLACLLDTRSSQMILVPTNRTDRAALFRCRTAVVKAGHDCVVVYASEVSDRLRGCYQSDLMRYWGAMKEAANFLDADLLFGIYTAAGNTLRSYQRKKLSQSQMCDHLRLLNSSSDEGIATPQNVLDWACYFTLPKRVAAEKAELRHYDKFITIAYVATDHTVHAQPMNPVADINHVLSACITDHANRTVEPWAIYTKRGQFCLPSLDGYDVLVTHDAKSLVLLLSGDAELQKFIKRGGRVWCVTLAEYLLEAQRCTTGSNGLYDLALRHGVQLPPSSRVGTPNDRLPFAFQRQFLLHAAPAVVKVFVEQLKRALEQCQVLSLAHRMDSLLAMASIEKAGIHIDAEEAARQTASLKSAASVLDAAMEAYVPSEVPVDMRIYFDWASLQQQHAYFFGGTISLGHQAHARDTPLWTSNVVHLCHRYGAFPHMVGELHLQRYAAQCALPTSGGLPNRVHQHIEAQGSQKLKTYRIVFFDIETTGLNPSTDAIVEVAMFDPIENSTFHTLVNPQRSITPRTVGIHHITNAMVRDAPTVDVVAKSIGQYLRLDKASYNPHEILVLVGHNVFSVDEPMLRRALECHAPECQLDGILFCDSLALLNAHRSELRWRMRTKRTNQPLMDALASSLRLSRLITALNVRPEGDLHRADTDTKALWYVLVNILGVADKDAVVQRDKILIEAANCFLRSPSIGCFVPAERQNRLVKVRLPGVAADYVRNVKLIASLQSKVFSETVLASLHAHGVKPAGLLLQRQLLDRHTSTFLQPSTGGRLAILHRDGRVHQHIDMTATTTSRTVSAYPSCQNIPKDDKSSVRRLFVSRFGSKGRCVEVDYSQLEIVVLAILCNDANLTKDLNSGVDFHVKRAAFFSGLPYDEIYQGYKRNVPKYVKLRKTAKQFSFQRLYGAGVPLLHKTTGIPVKDLEASIQRENEEYPGIAQFHRIIRSVALRPNNPGLPTSFIAEMPTGLRMSLRTRDVVLNLPPIKNYPIQGYGAELAQMMLGRLYRHFVRKDFYEDRAFLINFVHDSVWMDCHVDVLRECVTDTCRILGSVHEYVPKVFPGVKISVPLQVSASCGVDMCSMESIKDDDYTFVSKQRKTRPAEVQDFLDLTTAKSNFSAVMEESVASEEEESGETATSKAEATE

Target ORF information:

RefSeq Version	XM_001463998.1
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 putative mitochondrial DNA polymerase I protein B (POLIB), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001463998.1

ORF Insert Sequence:

ATGTTTCGTC GCTTGTCTAG CTCCTGGCCC CGCGCGCTGG TGCAACCACT GCGCTCAAGC 
TGCACTGTGC AGTCCCTGAC GAAGGCCCAG GCAGCGCTGG CAAGAGGGAA GGCTCCGTCA 
ATGGACGCCG CTGCGGAGTA CGCCGAGTTC TTTCGCGTCC CCATTTCCGT GGACGCCGGT 
GTCGTACGCA GCACGCAGGA ATACTACACC AACATCCGGA GGCTGGCAGC CGAGAATCAA 
AGCGCCGTTG CTCAGCGTAA CTCGCCGAAT AAGAGCTGCG GGGAAAGCAG CGTGGCGACT 
TTCCTGGTGG GGTACCACTT CCAGACAGCC ACCTTCCAGA TCTTTTCCAT CGCGGCGGAC 
ACCATTGTGT GTAGCGTGGA TACGAGGATG GAGGCTTCCG AGGTGTGGAG TGTAGCGGAT 
GAGCTCATGA CCCACATTAG CGGCGAATCA CCGCAGCTTG CTCTCATACC CGTTGTAGAG 
ACGGAACAGG AGCGGAAAAG GCTGCAGGAG CCTCTCCAAA AGCTCACGGC CTTGCTGAAG 
TTCAGCGGGC TGCAAGTGGC CACTCACGTT GACACCCTCG AGCTCAGCTC CGTGCTGTGC 
CACAGTCCTC CGTCGCCACA TGATATCACA CAGGACGCCT TGTCTGGTGT GTCTCCAGCT 
CCTATGACGT GCACGTGGTT CCAGGCGCAT TGGGCATTCA TCCGCACTGC AGCGTGCCAG 
GCGATGTCGG CAGACCCGTT GCAGACTGTG AGCACGTACG TGCTGCCCCG CTTCTCCGTC 
TACCTCATCC ATCTCGTGCG CGCCGGCACT CGTGGGCTTA ACGTGTCGCT CTGGGACCCG 
GTGAGCCGCC GCCGCTTCTC CGCCAAGGGC ATGCTCTCTG ACGTGCTTGC CTGCCTGCTC 
GATACGCGTT CCAGCCAGAT GATCTTGGTG CCCACGAACC GAACCGACCG CGCTGCACTC 
TTTCGCTGCC GCACCGCCGT CGTGAAGGCC GGCCACGATT GCGTCGTCGT ATACGCGTCA 
GAGGTGAGCG ACCGCCTTCG TGGCTGCTAC CAGTCCGACC TGATGCGGTA CTGGGGGGCG 
ATGAAGGAGG CTGCGAACTT TCTGGACGCG GATCTGCTCT TCGGCATCTA CACCGCAGCC 
GGCAACACAC TCCGCAGTTA TCAGCGCAAG AAGCTTTCCC AGAGCCAGAT GTGTGACCAC 
TTGCGTCTGC TAAACAGCTC CTCGGATGAG GGCATCGCGA CCCCACAGAA CGTCCTCGAC 
TGGGCCTGCT ACTTTACTCT CCCCAAGCGT GTTGCAGCTG AGAAGGCGGA GCTGCGCCAT 
TACGACAAGT TCATCACCAT CGCCTACGTC GCCACCGACC ACACCGTGCA TGCCCAGCCG 
ATGAACCCGG TGGCGGACAT CAACCACGTG CTGAGTGCGT GCATCACCGA CCACGCGAAC 
CGGACAGTAG AGCCGTGGGC CATCTACACG AAGCGCGGGC AGTTCTGCTT ACCCAGCCTC 
GATGGATACG ACGTGCTCGT CACACACGAT GCCAAGTCGC TTGTGCTGCT GCTGTCGGGG 
GATGCCGAGC TACAGAAGTT CATCAAGCGC GGCGGCCGTG TGTGGTGTGT GACGCTGGCC 
GAGTACCTTC TCGAGGCGCA GCGATGTACC ACTGGCAGCA ACGGCCTTTA CGATTTGGCC 
CTACGTCACG GTGTGCAGCT GCCACCATCG TCGCGCGTGG GTACGCCAAA CGACCGTCTC 
CCGTTTGCCT TTCAGCGGCA GTTTCTCCTC CATGCAGCGC CCGCGGTAGT GAAGGTGTTT 
GTGGAGCAGT TGAAGCGTGC GCTTGAACAA TGCCAGGTGC TGAGCTTGGC GCACCGCATG 
GACTCCCTGC TTGCGATGGC GAGCATCGAG AAGGCCGGCA TTCACATCGA TGCGGAGGAG 
GCGGCACGGC AGACGGCCTC GCTGAAGAGC GCCGCGTCTG TGCTGGACGC GGCGATGGAG 
GCGTACGTGC CGAGCGAGGT ACCGGTTGAC ATGAGGATTT ACTTTGACTG GGCGTCGCTG 
CAGCAGCAGC ACGCCTACTT CTTTGGTGGC ACAATTTCGC TAGGACACCA GGCCCACGCC 
CGCGATACCC CTCTATGGAC TTCGAATGTG GTCCATCTTT GCCACCGCTA CGGCGCTTTC 
CCACACATGG TTGGGGAGCT ACACCTGCAG CGTTACGCGG CCCAATGCGC TCTGCCCACG 
TCGGGAGGGC TGCCGAACCG CGTCCATCAG CACATCGAGG CGCAGGGCTC GCAGAAACTA 
AAAACGTACC GCATCGTCTT CTTTGACATC GAGACAACGG GGCTCAACCC AAGCACGGAC 
GCCATTGTGG AAGTGGCGAT GTTCGACCCG ATCGAGAACA GCACGTTTCA CACGCTCGTG 
AACCCGCAGC GTTCCATCAC TCCGCGAACT GTTGGGATTC ACCACATCAC AAACGCTATG 
GTGCGCGACG CGCCGACCGT GGACGTGGTC GCGAAGAGCA TTGGCCAGTA TCTGCGCCTC 
GACAAGGCCT CGTACAACCC GCATGAGATT TTGGTGCTGG TAGGCCACAA CGTCTTCTCC 
GTGGACGAGC CGATGCTGCG CCGCGCGCTC GAGTGTCACG CGCCGGAGTG CCAGCTGGAC 
GGCATTCTGT TTTGCGACTC ACTGGCGCTC CTTAATGCTC ACCGGAGCGA GCTGCGATGG 
CGCATGCGCA CCAAACGCAC GAACCAACCC CTCATGGATG CCCTCGCATC CTCTCTGCGC 
CTCAGTCGCC TGATCACAGC GCTCAACGTG CGGCCGGAAG GCGACCTGCA CCGCGCTGAC 
ACGGACACAA AGGCACTCTG GTATGTGCTG GTCAACATCC TCGGTGTCGC CGACAAAGAC 
GCTGTAGTGC AGCGGGACAA GATTTTGATC GAGGCCGCCA ACTGCTTCCT GCGCTCTCCG 
TCGATCGGGT GCTTTGTACC AGCAGAGCGT CAAAACCGGC TCGTGAAGGT GCGGCTGCCC 
GGCGTGGCTG CCGACTACGT CCGCAACGTG AAGCTGATTG CAAGTCTGCA GAGCAAAGTC 
TTCTCCGAAA CTGTGCTCGC GTCCTTGCAC GCGCACGGCG TGAAGCCGGC AGGCCTGCTC 
CTGCAGCGGC AGCTGCTCGA CCGTCACACC TCCACGTTCC TGCAACCGAG CACAGGCGGG 
CGGCTGGCCA TTCTGCACCG AGACGGCAGA GTGCACCAGC ACATCGACAT GACCGCCACG 
ACGACGTCGC GTACTGTTAG CGCCTACCCG AGTTGCCAGA ACATTCCGAA GGACGATAAG 
TCGTCTGTGC GGCGTCTCTT TGTGTCCCGC TTCGGCTCCA AGGGGCGTTG CGTCGAGGTG 
GACTACTCGC AGCTGGAGAT TGTGGTGCTG GCGATCCTGT GCAACGACGC AAACCTCACA 
AAAGACCTCA ATAGCGGTGT CGACTTCCAC GTGAAGCGGG CGGCGTTCTT TAGCGGGCTG 
CCGTACGACG AGATTTACCA GGGTTACAAG CGCAATGTCC CCAAGTACGT CAAGCTGCGC 
AAGACGGCGA AGCAGTTCTC CTTTCAACGT CTGTACGGCG CTGGGGTGCC GCTGTTGCAC 
AAGACCACCG GCATTCCCGT AAAGGACCTG GAGGCGTCGA TTCAGAGAGA GAATGAGGAG 
TACCCCGGCA TCGCGCAGTT TCACCGCATT ATCCGGTCGG TCGCGCTTCG CCCGAACAAC 
CCAGGACTGC CCACCAGCTT CATTGCCGAG ATGCCGACGG GGCTGCGGAT GAGCCTGCGC 
ACGCGGGATG TGGTGCTGAA CCTGCCTCCC ATCAAGAACT ACCCAATTCA AGGCTACGGC 
GCGGAGCTGG CGCAGATGAT GCTCGGCCGG CTCTACCGGC ACTTTGTGCG GAAAGACTTC 
TACGAGGACC GCGCGTTTCT CATCAACTTT GTTCACGACT CGGTGTGGAT GGACTGCCAC 
GTCGATGTGC TGCGGGAATG CGTAACGGAC ACATGCCGCA TCCTCGGCTC GGTGCACGAG 
TACGTCCCAA AGGTGTTCCC TGGAGTGAAG ATCAGCGTGC CGCTGCAAGT CTCTGCGTCG 
TGCGGGGTAG ACATGTGCTC CATGGAGAGC ATCAAAGACG ACGACTACAC GTTTGTGTCG 
AAGCAGCGCA AGACGAGGCC CGCAGAGGTG CAGGACTTTT TGGACCTGAC GACGGCCAAG 
TCGAACTTCT CGGCGGTGAT GGAGGAGAGC GTCGCGTCCG AGGAGGAGGA GAGCGGGGAA 
ACGGCGACGT CCAAGGCGGA GGCGACCGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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POLIB cDNA ORF clone, Leishmania infantum JPCM5

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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