LINJ_31_0420 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	LINJ_31_0420
Entrez Gene ID	5071424
Full Name	putative calpain-like protein
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_001467337.1	XP_001467374.1	putative calpain-like protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LINJ_31_0420 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LINJ_31_0420 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc04820	XM_001467337.1 Latest version!	Leishmania infantum JPCM5 putative calpain-like protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LINJ_31_0420 ( XM_001467337.1 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_001467374.1 Leishmania infantum JPCM5 putative calpain-like protein partial mRNA.

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CloneID	OLc04820
Clone ID Related Accession (Same CDS sequence)	XM_001467337.1
Accession Version	XM_001467337.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-07-15
Organism	Leishmania infantum JPCM5
Product	putative calpain-like protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009415). COMPLETENESS: incomplete on both ends.

ATGAGCGCGA CAAAGGCAGC GATCACAGAA TCCTCATCGA ACCTCGCGGC GGCCTCGGTT 
GTGTCAGAGG AGCGGCCCGA GACGCGGCAG TACGGTCTTA CCGACAGCCT GCACATCTCG 
CCTGCCAACC GCATGGCCGC AGAGAAGTTG GTGGCCTTTG TGCAGGCCGT CTCCGATCCG 
GAGGTGCTGC ACGTCATCTA CGACTACCTC GAGGAGCATG AGAAAGCGGT GCCGCGCTAC 
TGCCTCGCCA CGAAGGCGTG CCGTGAGCTT CTGGAGCGCA AAGAAGAGAT GCTGATGGCT 
TGCTTGTTCA CCAGCGGCGG TGCTCACGAG TCATCACAGC ACAGTGCAGT TGGTGCCCGC 
CGCCCCCGTA GTGTTTCAGT GCCGCAGCTC GTGGACTCGC CAGACAAGAA GGCGGAGACA 
GCAAGGGCAG GGGAAGGCCT GACGGAGGCG CAGCGCCTCT TGCAGCAGAT TGAGGAGACG 
GACGGCTGTG ACAAGGGAGA CGACGATGAT GCCGATATGG ATGAGTTTGA TGACCTCAAT 
TACTTCCCGG CGCCCTACAC ACCATCCAGC CGCTTCAATT TTGGACAGCC GAGCGTCTCC 
GGTCTCGTCA CTCGGGGTTT TTCGGAGGGC ATGCTGTACA AGATCAAGAC CCCAAAAGGC 
GAGTACCACT TCTACAACGA TACCCTGCAG GAGGTGATGG TTGTACGCGT GCAGTGCCAT 
CTGCGCGGCA ACGAGAAGAT CAACGAACGT GCCATGCTCT CCCCCGTCGC CAGTACCGGC 
GAGACGGAGA TTACGATCGC CGTATTGCCG GAGGAGACGA ACTTTTTCAT GAGTGGGGTG 
GCGCATATGC CTCACTTCAT GGCAAAGCGA GTGCCAGTGC CGCGCGATTA CGTCTCGCCG 
TCGGTGACGC AGTCGCTGCG CAAGATCAAC GCCGAGATCA ACGCTGTGCG CAAGGCACTT 
GGCAAGTGGA GCCGAGCCTC CGACCAGCAG GCGTACCTGA AGTGCTGTCT CAAGAACAAC 
CTCCGCTTCA CAGACCTCAA CTTCCGCCCG AGTTCGGAGA GCCTGTATCG CCCTGAGTTC 
GACGCGGTCG TCATACCGGC CGTCACATGG CGGCGGCCGG AGGAGTACAT CTACCTGGCC 
GAGGTGTCGC AGACGCGTCT ATTCCGCGGC GAGATTTCGT GTTTCCTGGT GAAGCAGGGT 
GACCTGAGCA ACCACACGGT GGTGGCTGCG ATGGCGGCGG TGGCGCAGTT CCCGGCCCAT 
GTGCGGTGGA TGTTCCGCCA CCCCGTGAGC GCGCACGTGG GCAAGATGGA GAGGTCGCAG 
GGCTGCTACC GAGTGACGCT GCTGCACAAT GGGTGGTGGC ACACCTACCT TGTCGACGAC 
TACGTGCCTG CGTCGCTGAG AGGACCGCTG TTTGCGAGCT GTGCCGAGGA CCCGCGCCGG 
CTGTGGGTGC AGCTGCTCGA AAAGGCGTAT GCCAAGTCGC TTGGCTCATA CGCGGCGACG 
TGCCTCGCCG ACGCCATGGA GGCCCTCGGC GACTTCACTG GATTCCCCGT ACGCTTTCTC 
GACTCCATGT GGGCGGCCGC CGCGCAGGGG CCGAGGGATG GCGCGTTCCG GTCGCTCTTT 
CGCTACCTCG AGCTCTGTGT CCGGGCCGGC TGCACGGTGC TTCTCTTCTC TCCGCCCCCC 
ACCGACGAGC AGTCGAGTGC GACCGTGGAC ATGTCCCGCA AGCGTTCCTC GCGTATCATG 
CCGGTGAAGG GCGCGGTGCC GCAGTTCCTG CCCGGCCACA TCTACTTCCT GCGCGACGTC 
GCCTTCTACG AGGCGCTAGA TCTGCGCATG GTCCAGCTGA AGAACCCGTG GACGTGGGAG 
ACAAAAAACA AGATCCACTA CGAGAAGAAG TGGAGGTACA CGACGTGGTA CGACCAGCCC 
GACCCCAGCC TCAGTGCCAT TGCGTCCGCC ACGACGAGTC TGCACGGACA GGTGTGCTCC 
CGCGTTCTGG CCTCGCACGC GCTCAGCCCT AGAGCCTGTA CGGACTTGTC ACCGGAGGCG 
GTCGCTCTAA ACGAGCGCAA GGGCACGATG TGGGTAGAAT GGGGGGAGGC GCTGACGGCG 
TTTGCGGGCG GCGGCGTGTG CTACACCATG TGGGACCGAC ACCGGTACCG TGTGCGGAAC 
GCCTTTGTGG AGGGCCGACC ACGGCTGGCG CTTGAGCTGA AGGCGCGGCG CAAAGTGGAG 
CTGCTCGTGA CGCTTTCGCT GGAGACGGTG AGCGAGACGC TCGACAACTT TGGCACCGTC 
GTCGCTGCCC GTCCCATCCC GCTCCACGGC ACGGCGCTCT CGGTGGTGCA CCGACAGGCG 
AACCAGTCGG CGAAGGTGGT CCGTGAGTCC TGCGACGACA TCGAGAGCGT GACAGGCTGC 
CGAACATACG TCGTGGCGCG TGATGTGTCC ATAAAGGTGG TCGTTGAGCC GAGCACGAGT 
AGCAACGGCG CCGCCGTACT GGTTGTCCCG CTGCTCGATC CAAGCGCCGT TAGCGCTGCC 
GCCGTCGGCG CCGGCAGCGG ATCCTCCTCG AGCGCCGCCT CGATAGCCGC TGCGCCCGAG 
GTGCGCTTTG TCGTGTCCAT CGTCAGCGAC GCCGTTGCGG GGGACGGGGA GGATTTGTCC 
GTCCACTTTG TGTCGCTGCG GCGCAGCTGC GGCGTCTTCA CGGACTCAAC CACGGCGTTC 
CCACTGGAGG GCGCCTCGCG CGTGACAGCA CAGTACCAAG TGTGCACCAA GGCAGGCGTT 
GCGACGTGCG AGGGGTCTCG CATCTCTGGC AACGAAACGA AGGGTATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001467374.1
CDS	1..2811
Translation	MSATKAAITESSSNLAAASVVSEERPETRQYGLTDSLHISPANRMAAEKLVAFVQAVSDPEVLHVIYDYLEEHEKAVPRYCLATKACRELLERKEEMLMACLFTSGGAHESSQHSAVGARRPRSVSVPQLVDSPDKKAETARAGEGLTEAQRLLQQIEETDGCDKGDDDDADMDEFDDLNYFPAPYTPSSRFNFGQPSVSGLVTRGFSEGMLYKIKTPKGEYHFYNDTLQEVMVVRVQCHLRGNEKINERAMLSPVASTGETEITIAVLPEETNFFMSGVAHMPHFMAKRVPVPRDYVSPSVTQSLRKINAEINAVRKALGKWSRASDQQAYLKCCLKNNLRFTDLNFRPSSESLYRPEFDAVVIPAVTWRRPEEYIYLAEVSQTRLFRGEISCFLVKQGDLSNHTVVAAMAAVAQFPAHVRWMFRHPVSAHVGKMERSQGCYRVTLLHNGWWHTYLVDDYVPASLRGPLFASCAEDPRRLWVQLLEKAYAKSLGSYAATCLADAMEALGDFTGFPVRFLDSMWAAAAQGPRDGAFRSLFRYLELCVRAGCTVLLFSPPPTDEQSSATVDMSRKRSSRIMPVKGAVPQFLPGHIYFLRDVAFYEALDLRMVQLKNPWTWETKNKIHYEKKWRYTTWYDQPDPSLSAIASATTSLHGQVCSRVLASHALSPRACTDLSPEAVALNERKGTMWVEWGEALTAFAGGGVCYTMWDRHRYRVRNAFVEGRPRLALELKARRKVELLVTLSLETVSETLDNFGTVVAARPIPLHGTALSVVHRQANQSAKVVRESCDDIESVTGCRTYVVARDVSIKVVVEPSTSSNGAAVLVVPLLDPSAVSAAAVGAGSGSSSSAASIAAAPEVRFVVSIVSDAVAGDGEDLSVHFVSLRRSCGVFTDSTTAFPLEGASRVTAQYQVCTKAGVATCEGSRISGNETKGM

Target ORF information:

RefSeq Version	XM_001467337.1
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 putative calpain-like protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001467337.1

ORF Insert Sequence:

ATGAGCGCGA CAAAGGCAGC GATCACAGAA TCCTCATCGA ACCTCGCGGC GGCCTCGGTT 
GTGTCAGAGG AGCGGCCCGA GACGCGGCAG TACGGTCTTA CCGACAGCCT GCACATCTCG 
CCTGCCAACC GCATGGCCGC AGAGAAGTTG GTGGCCTTTG TGCAGGCCGT CTCCGATCCG 
GAGGTGCTGC ACGTCATCTA CGACTACCTC GAGGAGCATG AGAAAGCGGT GCCGCGCTAC 
TGCCTCGCCA CGAAGGCGTG CCGTGAGCTT CTGGAGCGCA AAGAAGAGAT GCTGATGGCT 
TGCTTGTTCA CCAGCGGCGG TGCTCACGAG TCATCACAGC ACAGTGCAGT TGGTGCCCGC 
CGCCCCCGTA GTGTTTCAGT GCCGCAGCTC GTGGACTCGC CAGACAAGAA GGCGGAGACA 
GCAAGGGCAG GGGAAGGCCT GACGGAGGCG CAGCGCCTCT TGCAGCAGAT TGAGGAGACG 
GACGGCTGTG ACAAGGGAGA CGACGATGAT GCCGATATGG ATGAGTTTGA TGACCTCAAT 
TACTTCCCGG CGCCCTACAC ACCATCCAGC CGCTTCAATT TTGGACAGCC GAGCGTCTCC 
GGTCTCGTCA CTCGGGGTTT TTCGGAGGGC ATGCTGTACA AGATCAAGAC CCCAAAAGGC 
GAGTACCACT TCTACAACGA TACCCTGCAG GAGGTGATGG TTGTACGCGT GCAGTGCCAT 
CTGCGCGGCA ACGAGAAGAT CAACGAACGT GCCATGCTCT CCCCCGTCGC CAGTACCGGC 
GAGACGGAGA TTACGATCGC CGTATTGCCG GAGGAGACGA ACTTTTTCAT GAGTGGGGTG 
GCGCATATGC CTCACTTCAT GGCAAAGCGA GTGCCAGTGC CGCGCGATTA CGTCTCGCCG 
TCGGTGACGC AGTCGCTGCG CAAGATCAAC GCCGAGATCA ACGCTGTGCG CAAGGCACTT 
GGCAAGTGGA GCCGAGCCTC CGACCAGCAG GCGTACCTGA AGTGCTGTCT CAAGAACAAC 
CTCCGCTTCA CAGACCTCAA CTTCCGCCCG AGTTCGGAGA GCCTGTATCG CCCTGAGTTC 
GACGCGGTCG TCATACCGGC CGTCACATGG CGGCGGCCGG AGGAGTACAT CTACCTGGCC 
GAGGTGTCGC AGACGCGTCT ATTCCGCGGC GAGATTTCGT GTTTCCTGGT GAAGCAGGGT 
GACCTGAGCA ACCACACGGT GGTGGCTGCG ATGGCGGCGG TGGCGCAGTT CCCGGCCCAT 
GTGCGGTGGA TGTTCCGCCA CCCCGTGAGC GCGCACGTGG GCAAGATGGA GAGGTCGCAG 
GGCTGCTACC GAGTGACGCT GCTGCACAAT GGGTGGTGGC ACACCTACCT TGTCGACGAC 
TACGTGCCTG CGTCGCTGAG AGGACCGCTG TTTGCGAGCT GTGCCGAGGA CCCGCGCCGG 
CTGTGGGTGC AGCTGCTCGA AAAGGCGTAT GCCAAGTCGC TTGGCTCATA CGCGGCGACG 
TGCCTCGCCG ACGCCATGGA GGCCCTCGGC GACTTCACTG GATTCCCCGT ACGCTTTCTC 
GACTCCATGT GGGCGGCCGC CGCGCAGGGG CCGAGGGATG GCGCGTTCCG GTCGCTCTTT 
CGCTACCTCG AGCTCTGTGT CCGGGCCGGC TGCACGGTGC TTCTCTTCTC TCCGCCCCCC 
ACCGACGAGC AGTCGAGTGC GACCGTGGAC ATGTCCCGCA AGCGTTCCTC GCGTATCATG 
CCGGTGAAGG GCGCGGTGCC GCAGTTCCTG CCCGGCCACA TCTACTTCCT GCGCGACGTC 
GCCTTCTACG AGGCGCTAGA TCTGCGCATG GTCCAGCTGA AGAACCCGTG GACGTGGGAG 
ACAAAAAACA AGATCCACTA CGAGAAGAAG TGGAGGTACA CGACGTGGTA CGACCAGCCC 
GACCCCAGCC TCAGTGCCAT TGCGTCCGCC ACGACGAGTC TGCACGGACA GGTGTGCTCC 
CGCGTTCTGG CCTCGCACGC GCTCAGCCCT AGAGCCTGTA CGGACTTGTC ACCGGAGGCG 
GTCGCTCTAA ACGAGCGCAA GGGCACGATG TGGGTAGAAT GGGGGGAGGC GCTGACGGCG 
TTTGCGGGCG GCGGCGTGTG CTACACCATG TGGGACCGAC ACCGGTACCG TGTGCGGAAC 
GCCTTTGTGG AGGGCCGACC ACGGCTGGCG CTTGAGCTGA AGGCGCGGCG CAAAGTGGAG 
CTGCTCGTGA CGCTTTCGCT GGAGACGGTG AGCGAGACGC TCGACAACTT TGGCACCGTC 
GTCGCTGCCC GTCCCATCCC GCTCCACGGC ACGGCGCTCT CGGTGGTGCA CCGACAGGCG 
AACCAGTCGG CGAAGGTGGT CCGTGAGTCC TGCGACGACA TCGAGAGCGT GACAGGCTGC 
CGAACATACG TCGTGGCGCG TGATGTGTCC ATAAAGGTGG TCGTTGAGCC GAGCACGAGT 
AGCAACGGCG CCGCCGTACT GGTTGTCCCG CTGCTCGATC CAAGCGCCGT TAGCGCTGCC 
GCCGTCGGCG CCGGCAGCGG ATCCTCCTCG AGCGCCGCCT CGATAGCCGC TGCGCCCGAG 
GTGCGCTTTG TCGTGTCCAT CGTCAGCGAC GCCGTTGCGG GGGACGGGGA GGATTTGTCC 
GTCCACTTTG TGTCGCTGCG GCGCAGCTGC GGCGTCTTCA CGGACTCAAC CACGGCGTTC 
CCACTGGAGG GCGCCTCGCG CGTGACAGCA CAGTACCAAG TGTGCACCAA GGCAGGCGTT 
GCGACGTGCG AGGGGTCTCG CATCTCTGGC AACGAAACGA AGGGTATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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LINJ_31_0420 cDNA ORF clone, Leishmania infantum JPCM5

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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