ORF » Species Summary » Megachile rotundata » LOC100876845 cDNA ORF clone

LOC100876845 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

The following LOC100876845 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100876845 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMe110724 XM_012292211.1
Latest version!		 Megachile rotundata zinc finger MIZ domain-containing protein 1 (LOC100876845), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OMe110724 XM_012292209.1
Latest version!		 Megachile rotundata zinc finger MIZ domain-containing protein 1 (LOC100876845), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OMe110724 XM_012292208.1
Latest version!		 Megachile rotundata zinc finger MIZ domain-containing protein 1 (LOC100876845), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OMe110724 XM_012292210.1
Latest version!		 Megachile rotundata zinc finger MIZ domain-containing protein 1 (LOC100876845), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LOC100876845 ( XM_012292209.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012147599.1 Megachile rotundata zinc finger MIZ domain-containing protein 1 (LOC100876845), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OMe110724
    Clone ID Related Accession (Same CDS sequence) XM_012292209.1 , XM_012292211.1 , XM_012292208.1 , XM_012292210.1
    Accession Version XM_012292209.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2592bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-04-06
    Organism Megachile rotundata(alfalfa leafcutting bee)
    Product zinc finger MIZ domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797384.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGTGGCAG CTGCCACCGC TACGGCCACC GCCACTGCAA GCGTGGTGGC CATGCAGGAC 
    CGCCAAGACA TCCCCTCGCA ATTCAATCAG ATGCAGAGCC AACATGGAAT CCCCCACCAA 
    ACGTACAACG CAGGGTACGC CCAAAGAGGA CCAATGGCCG GAATGGGGCC AGTCGGGATG 
    AGCAACTTTA ACAGCATGGG CACGATGAGC CCAATGCACT CTATGAATTC CATGAATTCG 
    ATGAACAGTA TGAATGGCAT GAACTCTATG AATCCTATGT CGATGGGAGG TATGAACGGT 
    ATGACTGGTA TGAACGGAAT GACCCCCATG AGCTCCATGA GTAACATGGG TAATATGGGC 
    ATGAACAATA TGATGGGACC TAACAACATG CAGATGAACA AGATGAGCAT GCAGGCTCAA 
    CCACACCAGG GCTATCCAAG AAGACTAGCA CCTTACCCAA ACCCTGCGAT GCACATGGCG 
    CAAAAGAGGC AACAGGGTCC TTACCCGGCT CCCAACCCAG CCGCGATGCA ACCAACCTTC 
    AACGGCATGG CGACGTCGCA GTACCCGACC AATTACCCCG CGGCAGCGAG GCCGAATTTT 
    CAGCCTCAGT ATCAACCGAT GCAGAGCATG AATCCGTCGG CTGCCGGCTT TGGGCCTAAC 
    TCTATGATAC GAGGCGCCAA CATGAGGCAA ACAGCGCCTT CGTATCAGAC CGCGAGTCAG 
    GCGGCAACCA ACCAATACTA CGGTAGCAAT GGCATACCGG TTAGCATGGG ACCTACCACG 
    GTTGGCAATC AATTCGTTGG ACACCAACCG AACACGGGAT ACGCCGGTGC AGCGTCCTCG 
    TACGGGGCAA CCGGGGCTGT CACCAGTCAG TACCAACAGG ACGTCGCGGC GATGAGAACG 
    ACGGGAGCAG GGACACTGAG TTACCAGCAC AGCCCTATCC CTGGGAATCC GACACCTCCG 
    CTCACCCCGG CTACGAGTAT GCCCCCTTAC ATCAGTCCGA ATCCGGATAT CAAGCCTAAT 
    TTCAACGATA TGAAGTCACC GGTTAATATT CAGAAGGACG ATGAGTTAAG ATTAACATTC 
    CCCGTGAGGG ATGGCATCAT TCTTCCACCC TTCCGCTTAG AACATAATCT GGCTGTAAGC 
    AACCACGTAT TCCAACTGAA GTCCACGGTG CATCAAACGT TGATGTGGCG GTCCGACCTG 
    GAACTACAGC TCAAGTGTTT CCACCACGAA GACAGGCAAA TGAACACGAA CTGGCCCGCG 
    AGCGTGCAAG TATCCGTAAA CGCCACGCCG TTGGTTATCG ATCGTGGTGA AAATAAAACG 
    TCTCATAAGC CCTTATACCT GAAGGACGTT TGTCAATCAG GAAGGAACAC AATACAGATC 
    ACGGTGTCGG CGTGTTGTTG TTCCCATTTA TTCGTGCTAC AATTAGTTCA TCGACCAAGC 
    GTGCGAAGCG TGCTTCAGGG ATTGTTACGT AAGAGGCTGC TCACGGCGGA ACATTGTATC 
    ACTAAAATTA AACGAAATTT CAGTAATACT ATTTCCAATA ACGGTATACA GTCGGAGAAG 
    GATGTTGTAG AACAGACAGC ACTTAAGGTA TCCTTAAAAT GTCCTATTAC CTTTAAACGT 
    ATTACACTGC CAGCTAGGGG ACACGATTGT AAACATATTC AGTGCTTCGA TTTGGAGTCG 
    TACCTACAGC TAAATTGTGA AAGAGGATCG TGGAGATGTC CTGTATGCAC GAAACCTGCC 
    CAATTAGAAG GTTTAGAGGT TGACCAGTAC ATGTGGGGCA TACTGAACAC ATTAAACACG 
    GCGGAGGTTG AAGAAGTGAC GATAGACTCG GTCGCGAATT GGAAACCAGC GAAGAATTTA 
    ACTGGTATTA AGTCCGAAGA AGAAAGCGAT TGCAAGAGGA TGACGAAGGC CATGTCACCT 
    GGAAGTATGA ACATGCCAAC GATGAATAAT TGGGATATGA ATCAGGCAAT GAGTCCGTAC 
    ATACCGCCCG ACATGAGTAG CATTGTAAGC GGCTCTATGA TGAATAACAC ACCATCCACG 
    TACGCGAATA ATAACATCAA TCATCGGAAT TCGTCTGGAG GATCCTTCGA TATCAATTCC 
    GGAACGAATA CGAATACCAA TAATGATTAC ACAAACGGTG CAGGTCCACT GTCGCATCTG 
    AATGAATCAG TCAACTCTTT AGACCCTCTC AACGCTATGG AGAAATCGCT TAACGATCAG 
    ATGCCTCATA CACCACATAC ACCTCACACC CCCCATACGC CCCACACACC AGGTGGTGGA 
    AATAGTGGTC CACCGAGTGT TCCCCCTGCA TCTCAGGAAT CCACGGGAAA TCATAATACA 
    TCTGGTAATA CGAGTACAAA CATAAATAAC GATACTGCAG ACATACCATC TGACTTAAAT 
    TTCGACCCAG CTGCAGTAAT CGATGGCGAG GGTACTGGTC AAGAGGCATT GAATCTTTTG 
    CCAGACAATG TTGTGGATCC AATGGAACTC CTGTCGTATC TGGATCCACC AGATCTGAAC 
    ACCCCTCCCA GTAGCGGCGC TAGCAGTGGT AACCCCTCAT CAAGTGACGA TATATTAGCA 
    CTCTTCGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012147599.1
    CDS1029..3620
    Translation

    Target ORF information:

    RefSeq Version XM_012292209.1
    Organism Megachile rotundata(alfalfa leafcutting bee)
    Definition Megachile rotundata zinc finger MIZ domain-containing protein 1 (LOC100876845), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012292209.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGTGGCAG CTGCCACCGC TACGGCCACC GCCACTGCAA GCGTGGTGGC CATGCAGGAC 
    CGCCAAGACA TCCCCTCGCA ATTCAATCAG ATGCAGAGCC AACATGGAAT CCCCCACCAA 
    ACGTACAACG CAGGGTACGC CCAAAGAGGA CCAATGGCCG GAATGGGGCC AGTCGGGATG 
    AGCAACTTTA ACAGCATGGG CACGATGAGC CCAATGCACT CTATGAATTC CATGAATTCG 
    ATGAACAGTA TGAATGGCAT GAACTCTATG AATCCTATGT CGATGGGAGG TATGAACGGT 
    ATGACTGGTA TGAACGGAAT GACCCCCATG AGCTCCATGA GTAACATGGG TAATATGGGC 
    ATGAACAATA TGATGGGACC TAACAACATG CAGATGAACA AGATGAGCAT GCAGGCTCAA 
    CCACACCAGG GCTATCCAAG AAGACTAGCA CCTTACCCAA ACCCTGCGAT GCACATGGCG 
    CAAAAGAGGC AACAGGGTCC TTACCCGGCT CCCAACCCAG CCGCGATGCA ACCAACCTTC 
    AACGGCATGG CGACGTCGCA GTACCCGACC AATTACCCCG CGGCAGCGAG GCCGAATTTT 
    CAGCCTCAGT ATCAACCGAT GCAGAGCATG AATCCGTCGG CTGCCGGCTT TGGGCCTAAC 
    TCTATGATAC GAGGCGCCAA CATGAGGCAA ACAGCGCCTT CGTATCAGAC CGCGAGTCAG 
    GCGGCAACCA ACCAATACTA CGGTAGCAAT GGCATACCGG TTAGCATGGG ACCTACCACG 
    GTTGGCAATC AATTCGTTGG ACACCAACCG AACACGGGAT ACGCCGGTGC AGCGTCCTCG 
    TACGGGGCAA CCGGGGCTGT CACCAGTCAG TACCAACAGG ACGTCGCGGC GATGAGAACG 
    ACGGGAGCAG GGACACTGAG TTACCAGCAC AGCCCTATCC CTGGGAATCC GACACCTCCG 
    CTCACCCCGG CTACGAGTAT GCCCCCTTAC ATCAGTCCGA ATCCGGATAT CAAGCCTAAT 
    TTCAACGATA TGAAGTCACC GGTTAATATT CAGAAGGACG ATGAGTTAAG ATTAACATTC 
    CCCGTGAGGG ATGGCATCAT TCTTCCACCC TTCCGCTTAG AACATAATCT GGCTGTAAGC 
    AACCACGTAT TCCAACTGAA GTCCACGGTG CATCAAACGT TGATGTGGCG GTCCGACCTG 
    GAACTACAGC TCAAGTGTTT CCACCACGAA GACAGGCAAA TGAACACGAA CTGGCCCGCG 
    AGCGTGCAAG TATCCGTAAA CGCCACGCCG TTGGTTATCG ATCGTGGTGA AAATAAAACG 
    TCTCATAAGC CCTTATACCT GAAGGACGTT TGTCAATCAG GAAGGAACAC AATACAGATC 
    ACGGTGTCGG CGTGTTGTTG TTCCCATTTA TTCGTGCTAC AATTAGTTCA TCGACCAAGC 
    GTGCGAAGCG TGCTTCAGGG ATTGTTACGT AAGAGGCTGC TCACGGCGGA ACATTGTATC 
    ACTAAAATTA AACGAAATTT CAGTAATACT ATTTCCAATA ACGGTATACA GTCGGAGAAG 
    GATGTTGTAG AACAGACAGC ACTTAAGGTA TCCTTAAAAT GTCCTATTAC CTTTAAACGT 
    ATTACACTGC CAGCTAGGGG ACACGATTGT AAACATATTC AGTGCTTCGA TTTGGAGTCG 
    TACCTACAGC TAAATTGTGA AAGAGGATCG TGGAGATGTC CTGTATGCAC GAAACCTGCC 
    CAATTAGAAG GTTTAGAGGT TGACCAGTAC ATGTGGGGCA TACTGAACAC ATTAAACACG 
    GCGGAGGTTG AAGAAGTGAC GATAGACTCG GTCGCGAATT GGAAACCAGC GAAGAATTTA 
    ACTGGTATTA AGTCCGAAGA AGAAAGCGAT TGCAAGAGGA TGACGAAGGC CATGTCACCT 
    GGAAGTATGA ACATGCCAAC GATGAATAAT TGGGATATGA ATCAGGCAAT GAGTCCGTAC 
    ATACCGCCCG ACATGAGTAG CATTGTAAGC GGCTCTATGA TGAATAACAC ACCATCCACG 
    TACGCGAATA ATAACATCAA TCATCGGAAT TCGTCTGGAG GATCCTTCGA TATCAATTCC 
    GGAACGAATA CGAATACCAA TAATGATTAC ACAAACGGTG CAGGTCCACT GTCGCATCTG 
    AATGAATCAG TCAACTCTTT AGACCCTCTC AACGCTATGG AGAAATCGCT TAACGATCAG 
    ATGCCTCATA CACCACATAC ACCTCACACC CCCCATACGC CCCACACACC AGGTGGTGGA 
    AATAGTGGTC CACCGAGTGT TCCCCCTGCA TCTCAGGAAT CCACGGGAAA TCATAATACA 
    TCTGGTAATA CGAGTACAAA CATAAATAAC GATACTGCAG ACATACCATC TGACTTAAAT 
    TTCGACCCAG CTGCAGTAAT CGATGGCGAG GGTACTGGTC AAGAGGCATT GAATCTTTTG 
    CCAGACAATG TTGTGGATCC AATGGAACTC CTGTCGTATC TGGATCCACC AGATCTGAAC 
    ACCCCTCCCA GTAGCGGCGC TAGCAGTGGT AACCCCTCAT CAAGTGACGA TATATTAGCA 
    CTCTTCGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC100876845 ( XM_012292211.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012147601.1 Megachile rotundata zinc finger MIZ domain-containing protein 1 (LOC100876845), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID OMe110724
    Clone ID Related Accession (Same CDS sequence) XM_012292209.1 , XM_012292211.1 , XM_012292208.1 , XM_012292210.1
    Accession Version XM_012292211.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2592bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-04-06
    Organism Megachile rotundata(alfalfa leafcutting bee)
    Product zinc finger MIZ domain-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797384.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGTGGCAG CTGCCACCGC TACGGCCACC GCCACTGCAA GCGTGGTGGC CATGCAGGAC 
    CGCCAAGACA TCCCCTCGCA ATTCAATCAG ATGCAGAGCC AACATGGAAT CCCCCACCAA 
    ACGTACAACG CAGGGTACGC CCAAAGAGGA CCAATGGCCG GAATGGGGCC AGTCGGGATG 
    AGCAACTTTA ACAGCATGGG CACGATGAGC CCAATGCACT CTATGAATTC CATGAATTCG 
    ATGAACAGTA TGAATGGCAT GAACTCTATG AATCCTATGT CGATGGGAGG TATGAACGGT 
    ATGACTGGTA TGAACGGAAT GACCCCCATG AGCTCCATGA GTAACATGGG TAATATGGGC 
    ATGAACAATA TGATGGGACC TAACAACATG CAGATGAACA AGATGAGCAT GCAGGCTCAA 
    CCACACCAGG GCTATCCAAG AAGACTAGCA CCTTACCCAA ACCCTGCGAT GCACATGGCG 
    CAAAAGAGGC AACAGGGTCC TTACCCGGCT CCCAACCCAG CCGCGATGCA ACCAACCTTC 
    AACGGCATGG CGACGTCGCA GTACCCGACC AATTACCCCG CGGCAGCGAG GCCGAATTTT 
    CAGCCTCAGT ATCAACCGAT GCAGAGCATG AATCCGTCGG CTGCCGGCTT TGGGCCTAAC 
    TCTATGATAC GAGGCGCCAA CATGAGGCAA ACAGCGCCTT CGTATCAGAC CGCGAGTCAG 
    GCGGCAACCA ACCAATACTA CGGTAGCAAT GGCATACCGG TTAGCATGGG ACCTACCACG 
    GTTGGCAATC AATTCGTTGG ACACCAACCG AACACGGGAT ACGCCGGTGC AGCGTCCTCG 
    TACGGGGCAA CCGGGGCTGT CACCAGTCAG TACCAACAGG ACGTCGCGGC GATGAGAACG 
    ACGGGAGCAG GGACACTGAG TTACCAGCAC AGCCCTATCC CTGGGAATCC GACACCTCCG 
    CTCACCCCGG CTACGAGTAT GCCCCCTTAC ATCAGTCCGA ATCCGGATAT CAAGCCTAAT 
    TTCAACGATA TGAAGTCACC GGTTAATATT CAGAAGGACG ATGAGTTAAG ATTAACATTC 
    CCCGTGAGGG ATGGCATCAT TCTTCCACCC TTCCGCTTAG AACATAATCT GGCTGTAAGC 
    AACCACGTAT TCCAACTGAA GTCCACGGTG CATCAAACGT TGATGTGGCG GTCCGACCTG 
    GAACTACAGC TCAAGTGTTT CCACCACGAA GACAGGCAAA TGAACACGAA CTGGCCCGCG 
    AGCGTGCAAG TATCCGTAAA CGCCACGCCG TTGGTTATCG ATCGTGGTGA AAATAAAACG 
    TCTCATAAGC CCTTATACCT GAAGGACGTT TGTCAATCAG GAAGGAACAC AATACAGATC 
    ACGGTGTCGG CGTGTTGTTG TTCCCATTTA TTCGTGCTAC AATTAGTTCA TCGACCAAGC 
    GTGCGAAGCG TGCTTCAGGG ATTGTTACGT AAGAGGCTGC TCACGGCGGA ACATTGTATC 
    ACTAAAATTA AACGAAATTT CAGTAATACT ATTTCCAATA ACGGTATACA GTCGGAGAAG 
    GATGTTGTAG AACAGACAGC ACTTAAGGTA TCCTTAAAAT GTCCTATTAC CTTTAAACGT 
    ATTACACTGC CAGCTAGGGG ACACGATTGT AAACATATTC AGTGCTTCGA TTTGGAGTCG 
    TACCTACAGC TAAATTGTGA AAGAGGATCG TGGAGATGTC CTGTATGCAC GAAACCTGCC 
    CAATTAGAAG GTTTAGAGGT TGACCAGTAC ATGTGGGGCA TACTGAACAC ATTAAACACG 
    GCGGAGGTTG AAGAAGTGAC GATAGACTCG GTCGCGAATT GGAAACCAGC GAAGAATTTA 
    ACTGGTATTA AGTCCGAAGA AGAAAGCGAT TGCAAGAGGA TGACGAAGGC CATGTCACCT 
    GGAAGTATGA ACATGCCAAC GATGAATAAT TGGGATATGA ATCAGGCAAT GAGTCCGTAC 
    ATACCGCCCG ACATGAGTAG CATTGTAAGC GGCTCTATGA TGAATAACAC ACCATCCACG 
    TACGCGAATA ATAACATCAA TCATCGGAAT TCGTCTGGAG GATCCTTCGA TATCAATTCC 
    GGAACGAATA CGAATACCAA TAATGATTAC ACAAACGGTG CAGGTCCACT GTCGCATCTG 
    AATGAATCAG TCAACTCTTT AGACCCTCTC AACGCTATGG AGAAATCGCT TAACGATCAG 
    ATGCCTCATA CACCACATAC ACCTCACACC CCCCATACGC CCCACACACC AGGTGGTGGA 
    AATAGTGGTC CACCGAGTGT TCCCCCTGCA TCTCAGGAAT CCACGGGAAA TCATAATACA 
    TCTGGTAATA CGAGTACAAA CATAAATAAC GATACTGCAG ACATACCATC TGACTTAAAT 
    TTCGACCCAG CTGCAGTAAT CGATGGCGAG GGTACTGGTC AAGAGGCATT GAATCTTTTG 
    CCAGACAATG TTGTGGATCC AATGGAACTC CTGTCGTATC TGGATCCACC AGATCTGAAC 
    ACCCCTCCCA GTAGCGGCGC TAGCAGTGGT AACCCCTCAT CAAGTGACGA TATATTAGCA 
    CTCTTCGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012147601.1
    CDS1033..3624
    Translation

    Target ORF information:

    RefSeq Version XM_012292211.1
    Organism Megachile rotundata(alfalfa leafcutting bee)
    Definition Megachile rotundata zinc finger MIZ domain-containing protein 1 (LOC100876845), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012292211.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGTGGCAG CTGCCACCGC TACGGCCACC GCCACTGCAA GCGTGGTGGC CATGCAGGAC 
    CGCCAAGACA TCCCCTCGCA ATTCAATCAG ATGCAGAGCC AACATGGAAT CCCCCACCAA 
    ACGTACAACG CAGGGTACGC CCAAAGAGGA CCAATGGCCG GAATGGGGCC AGTCGGGATG 
    AGCAACTTTA ACAGCATGGG CACGATGAGC CCAATGCACT CTATGAATTC CATGAATTCG 
    ATGAACAGTA TGAATGGCAT GAACTCTATG AATCCTATGT CGATGGGAGG TATGAACGGT 
    ATGACTGGTA TGAACGGAAT GACCCCCATG AGCTCCATGA GTAACATGGG TAATATGGGC 
    ATGAACAATA TGATGGGACC TAACAACATG CAGATGAACA AGATGAGCAT GCAGGCTCAA 
    CCACACCAGG GCTATCCAAG AAGACTAGCA CCTTACCCAA ACCCTGCGAT GCACATGGCG 
    CAAAAGAGGC AACAGGGTCC TTACCCGGCT CCCAACCCAG CCGCGATGCA ACCAACCTTC 
    AACGGCATGG CGACGTCGCA GTACCCGACC AATTACCCCG CGGCAGCGAG GCCGAATTTT 
    CAGCCTCAGT ATCAACCGAT GCAGAGCATG AATCCGTCGG CTGCCGGCTT TGGGCCTAAC 
    TCTATGATAC GAGGCGCCAA CATGAGGCAA ACAGCGCCTT CGTATCAGAC CGCGAGTCAG 
    GCGGCAACCA ACCAATACTA CGGTAGCAAT GGCATACCGG TTAGCATGGG ACCTACCACG 
    GTTGGCAATC AATTCGTTGG ACACCAACCG AACACGGGAT ACGCCGGTGC AGCGTCCTCG 
    TACGGGGCAA CCGGGGCTGT CACCAGTCAG TACCAACAGG ACGTCGCGGC GATGAGAACG 
    ACGGGAGCAG GGACACTGAG TTACCAGCAC AGCCCTATCC CTGGGAATCC GACACCTCCG 
    CTCACCCCGG CTACGAGTAT GCCCCCTTAC ATCAGTCCGA ATCCGGATAT CAAGCCTAAT 
    TTCAACGATA TGAAGTCACC GGTTAATATT CAGAAGGACG ATGAGTTAAG ATTAACATTC 
    CCCGTGAGGG ATGGCATCAT TCTTCCACCC TTCCGCTTAG AACATAATCT GGCTGTAAGC 
    AACCACGTAT TCCAACTGAA GTCCACGGTG CATCAAACGT TGATGTGGCG GTCCGACCTG 
    GAACTACAGC TCAAGTGTTT CCACCACGAA GACAGGCAAA TGAACACGAA CTGGCCCGCG 
    AGCGTGCAAG TATCCGTAAA CGCCACGCCG TTGGTTATCG ATCGTGGTGA AAATAAAACG 
    TCTCATAAGC CCTTATACCT GAAGGACGTT TGTCAATCAG GAAGGAACAC AATACAGATC 
    ACGGTGTCGG CGTGTTGTTG TTCCCATTTA TTCGTGCTAC AATTAGTTCA TCGACCAAGC 
    GTGCGAAGCG TGCTTCAGGG ATTGTTACGT AAGAGGCTGC TCACGGCGGA ACATTGTATC 
    ACTAAAATTA AACGAAATTT CAGTAATACT ATTTCCAATA ACGGTATACA GTCGGAGAAG 
    GATGTTGTAG AACAGACAGC ACTTAAGGTA TCCTTAAAAT GTCCTATTAC CTTTAAACGT 
    ATTACACTGC CAGCTAGGGG ACACGATTGT AAACATATTC AGTGCTTCGA TTTGGAGTCG 
    TACCTACAGC TAAATTGTGA AAGAGGATCG TGGAGATGTC CTGTATGCAC GAAACCTGCC 
    CAATTAGAAG GTTTAGAGGT TGACCAGTAC ATGTGGGGCA TACTGAACAC ATTAAACACG 
    GCGGAGGTTG AAGAAGTGAC GATAGACTCG GTCGCGAATT GGAAACCAGC GAAGAATTTA 
    ACTGGTATTA AGTCCGAAGA AGAAAGCGAT TGCAAGAGGA TGACGAAGGC CATGTCACCT 
    GGAAGTATGA ACATGCCAAC GATGAATAAT TGGGATATGA ATCAGGCAAT GAGTCCGTAC 
    ATACCGCCCG ACATGAGTAG CATTGTAAGC GGCTCTATGA TGAATAACAC ACCATCCACG 
    TACGCGAATA ATAACATCAA TCATCGGAAT TCGTCTGGAG GATCCTTCGA TATCAATTCC 
    GGAACGAATA CGAATACCAA TAATGATTAC ACAAACGGTG CAGGTCCACT GTCGCATCTG 
    AATGAATCAG TCAACTCTTT AGACCCTCTC AACGCTATGG AGAAATCGCT TAACGATCAG 
    ATGCCTCATA CACCACATAC ACCTCACACC CCCCATACGC CCCACACACC AGGTGGTGGA 
    AATAGTGGTC CACCGAGTGT TCCCCCTGCA TCTCAGGAAT CCACGGGAAA TCATAATACA 
    TCTGGTAATA CGAGTACAAA CATAAATAAC GATACTGCAG ACATACCATC TGACTTAAAT 
    TTCGACCCAG CTGCAGTAAT CGATGGCGAG GGTACTGGTC AAGAGGCATT GAATCTTTTG 
    CCAGACAATG TTGTGGATCC AATGGAACTC CTGTCGTATC TGGATCCACC AGATCTGAAC 
    ACCCCTCCCA GTAGCGGCGC TAGCAGTGGT AACCCCTCAT CAAGTGACGA TATATTAGCA 
    CTCTTCGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC100876845 ( XM_012292208.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012147598.1 Megachile rotundata zinc finger MIZ domain-containing protein 1 (LOC100876845), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OMe110724
    Clone ID Related Accession (Same CDS sequence) XM_012292209.1 , XM_012292211.1 , XM_012292208.1 , XM_012292210.1
    Accession Version XM_012292208.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2592bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-04-06
    Organism Megachile rotundata(alfalfa leafcutting bee)
    Product zinc finger MIZ domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797384.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGTGGCAG CTGCCACCGC TACGGCCACC GCCACTGCAA GCGTGGTGGC CATGCAGGAC 
    CGCCAAGACA TCCCCTCGCA ATTCAATCAG ATGCAGAGCC AACATGGAAT CCCCCACCAA 
    ACGTACAACG CAGGGTACGC CCAAAGAGGA CCAATGGCCG GAATGGGGCC AGTCGGGATG 
    AGCAACTTTA ACAGCATGGG CACGATGAGC CCAATGCACT CTATGAATTC CATGAATTCG 
    ATGAACAGTA TGAATGGCAT GAACTCTATG AATCCTATGT CGATGGGAGG TATGAACGGT 
    ATGACTGGTA TGAACGGAAT GACCCCCATG AGCTCCATGA GTAACATGGG TAATATGGGC 
    ATGAACAATA TGATGGGACC TAACAACATG CAGATGAACA AGATGAGCAT GCAGGCTCAA 
    CCACACCAGG GCTATCCAAG AAGACTAGCA CCTTACCCAA ACCCTGCGAT GCACATGGCG 
    CAAAAGAGGC AACAGGGTCC TTACCCGGCT CCCAACCCAG CCGCGATGCA ACCAACCTTC 
    AACGGCATGG CGACGTCGCA GTACCCGACC AATTACCCCG CGGCAGCGAG GCCGAATTTT 
    CAGCCTCAGT ATCAACCGAT GCAGAGCATG AATCCGTCGG CTGCCGGCTT TGGGCCTAAC 
    TCTATGATAC GAGGCGCCAA CATGAGGCAA ACAGCGCCTT CGTATCAGAC CGCGAGTCAG 
    GCGGCAACCA ACCAATACTA CGGTAGCAAT GGCATACCGG TTAGCATGGG ACCTACCACG 
    GTTGGCAATC AATTCGTTGG ACACCAACCG AACACGGGAT ACGCCGGTGC AGCGTCCTCG 
    TACGGGGCAA CCGGGGCTGT CACCAGTCAG TACCAACAGG ACGTCGCGGC GATGAGAACG 
    ACGGGAGCAG GGACACTGAG TTACCAGCAC AGCCCTATCC CTGGGAATCC GACACCTCCG 
    CTCACCCCGG CTACGAGTAT GCCCCCTTAC ATCAGTCCGA ATCCGGATAT CAAGCCTAAT 
    TTCAACGATA TGAAGTCACC GGTTAATATT CAGAAGGACG ATGAGTTAAG ATTAACATTC 
    CCCGTGAGGG ATGGCATCAT TCTTCCACCC TTCCGCTTAG AACATAATCT GGCTGTAAGC 
    AACCACGTAT TCCAACTGAA GTCCACGGTG CATCAAACGT TGATGTGGCG GTCCGACCTG 
    GAACTACAGC TCAAGTGTTT CCACCACGAA GACAGGCAAA TGAACACGAA CTGGCCCGCG 
    AGCGTGCAAG TATCCGTAAA CGCCACGCCG TTGGTTATCG ATCGTGGTGA AAATAAAACG 
    TCTCATAAGC CCTTATACCT GAAGGACGTT TGTCAATCAG GAAGGAACAC AATACAGATC 
    ACGGTGTCGG CGTGTTGTTG TTCCCATTTA TTCGTGCTAC AATTAGTTCA TCGACCAAGC 
    GTGCGAAGCG TGCTTCAGGG ATTGTTACGT AAGAGGCTGC TCACGGCGGA ACATTGTATC 
    ACTAAAATTA AACGAAATTT CAGTAATACT ATTTCCAATA ACGGTATACA GTCGGAGAAG 
    GATGTTGTAG AACAGACAGC ACTTAAGGTA TCCTTAAAAT GTCCTATTAC CTTTAAACGT 
    ATTACACTGC CAGCTAGGGG ACACGATTGT AAACATATTC AGTGCTTCGA TTTGGAGTCG 
    TACCTACAGC TAAATTGTGA AAGAGGATCG TGGAGATGTC CTGTATGCAC GAAACCTGCC 
    CAATTAGAAG GTTTAGAGGT TGACCAGTAC ATGTGGGGCA TACTGAACAC ATTAAACACG 
    GCGGAGGTTG AAGAAGTGAC GATAGACTCG GTCGCGAATT GGAAACCAGC GAAGAATTTA 
    ACTGGTATTA AGTCCGAAGA AGAAAGCGAT TGCAAGAGGA TGACGAAGGC CATGTCACCT 
    GGAAGTATGA ACATGCCAAC GATGAATAAT TGGGATATGA ATCAGGCAAT GAGTCCGTAC 
    ATACCGCCCG ACATGAGTAG CATTGTAAGC GGCTCTATGA TGAATAACAC ACCATCCACG 
    TACGCGAATA ATAACATCAA TCATCGGAAT TCGTCTGGAG GATCCTTCGA TATCAATTCC 
    GGAACGAATA CGAATACCAA TAATGATTAC ACAAACGGTG CAGGTCCACT GTCGCATCTG 
    AATGAATCAG TCAACTCTTT AGACCCTCTC AACGCTATGG AGAAATCGCT TAACGATCAG 
    ATGCCTCATA CACCACATAC ACCTCACACC CCCCATACGC CCCACACACC AGGTGGTGGA 
    AATAGTGGTC CACCGAGTGT TCCCCCTGCA TCTCAGGAAT CCACGGGAAA TCATAATACA 
    TCTGGTAATA CGAGTACAAA CATAAATAAC GATACTGCAG ACATACCATC TGACTTAAAT 
    TTCGACCCAG CTGCAGTAAT CGATGGCGAG GGTACTGGTC AAGAGGCATT GAATCTTTTG 
    CCAGACAATG TTGTGGATCC AATGGAACTC CTGTCGTATC TGGATCCACC AGATCTGAAC 
    ACCCCTCCCA GTAGCGGCGC TAGCAGTGGT AACCCCTCAT CAAGTGACGA TATATTAGCA 
    CTCTTCGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012147598.1
    CDS1032..3623
    Translation

    Target ORF information:

    RefSeq Version XM_012292208.1
    Organism Megachile rotundata(alfalfa leafcutting bee)
    Definition Megachile rotundata zinc finger MIZ domain-containing protein 1 (LOC100876845), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012292208.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGTGGCAG CTGCCACCGC TACGGCCACC GCCACTGCAA GCGTGGTGGC CATGCAGGAC 
    CGCCAAGACA TCCCCTCGCA ATTCAATCAG ATGCAGAGCC AACATGGAAT CCCCCACCAA 
    ACGTACAACG CAGGGTACGC CCAAAGAGGA CCAATGGCCG GAATGGGGCC AGTCGGGATG 
    AGCAACTTTA ACAGCATGGG CACGATGAGC CCAATGCACT CTATGAATTC CATGAATTCG 
    ATGAACAGTA TGAATGGCAT GAACTCTATG AATCCTATGT CGATGGGAGG TATGAACGGT 
    ATGACTGGTA TGAACGGAAT GACCCCCATG AGCTCCATGA GTAACATGGG TAATATGGGC 
    ATGAACAATA TGATGGGACC TAACAACATG CAGATGAACA AGATGAGCAT GCAGGCTCAA 
    CCACACCAGG GCTATCCAAG AAGACTAGCA CCTTACCCAA ACCCTGCGAT GCACATGGCG 
    CAAAAGAGGC AACAGGGTCC TTACCCGGCT CCCAACCCAG CCGCGATGCA ACCAACCTTC 
    AACGGCATGG CGACGTCGCA GTACCCGACC AATTACCCCG CGGCAGCGAG GCCGAATTTT 
    CAGCCTCAGT ATCAACCGAT GCAGAGCATG AATCCGTCGG CTGCCGGCTT TGGGCCTAAC 
    TCTATGATAC GAGGCGCCAA CATGAGGCAA ACAGCGCCTT CGTATCAGAC CGCGAGTCAG 
    GCGGCAACCA ACCAATACTA CGGTAGCAAT GGCATACCGG TTAGCATGGG ACCTACCACG 
    GTTGGCAATC AATTCGTTGG ACACCAACCG AACACGGGAT ACGCCGGTGC AGCGTCCTCG 
    TACGGGGCAA CCGGGGCTGT CACCAGTCAG TACCAACAGG ACGTCGCGGC GATGAGAACG 
    ACGGGAGCAG GGACACTGAG TTACCAGCAC AGCCCTATCC CTGGGAATCC GACACCTCCG 
    CTCACCCCGG CTACGAGTAT GCCCCCTTAC ATCAGTCCGA ATCCGGATAT CAAGCCTAAT 
    TTCAACGATA TGAAGTCACC GGTTAATATT CAGAAGGACG ATGAGTTAAG ATTAACATTC 
    CCCGTGAGGG ATGGCATCAT TCTTCCACCC TTCCGCTTAG AACATAATCT GGCTGTAAGC 
    AACCACGTAT TCCAACTGAA GTCCACGGTG CATCAAACGT TGATGTGGCG GTCCGACCTG 
    GAACTACAGC TCAAGTGTTT CCACCACGAA GACAGGCAAA TGAACACGAA CTGGCCCGCG 
    AGCGTGCAAG TATCCGTAAA CGCCACGCCG TTGGTTATCG ATCGTGGTGA AAATAAAACG 
    TCTCATAAGC CCTTATACCT GAAGGACGTT TGTCAATCAG GAAGGAACAC AATACAGATC 
    ACGGTGTCGG CGTGTTGTTG TTCCCATTTA TTCGTGCTAC AATTAGTTCA TCGACCAAGC 
    GTGCGAAGCG TGCTTCAGGG ATTGTTACGT AAGAGGCTGC TCACGGCGGA ACATTGTATC 
    ACTAAAATTA AACGAAATTT CAGTAATACT ATTTCCAATA ACGGTATACA GTCGGAGAAG 
    GATGTTGTAG AACAGACAGC ACTTAAGGTA TCCTTAAAAT GTCCTATTAC CTTTAAACGT 
    ATTACACTGC CAGCTAGGGG ACACGATTGT AAACATATTC AGTGCTTCGA TTTGGAGTCG 
    TACCTACAGC TAAATTGTGA AAGAGGATCG TGGAGATGTC CTGTATGCAC GAAACCTGCC 
    CAATTAGAAG GTTTAGAGGT TGACCAGTAC ATGTGGGGCA TACTGAACAC ATTAAACACG 
    GCGGAGGTTG AAGAAGTGAC GATAGACTCG GTCGCGAATT GGAAACCAGC GAAGAATTTA 
    ACTGGTATTA AGTCCGAAGA AGAAAGCGAT TGCAAGAGGA TGACGAAGGC CATGTCACCT 
    GGAAGTATGA ACATGCCAAC GATGAATAAT TGGGATATGA ATCAGGCAAT GAGTCCGTAC 
    ATACCGCCCG ACATGAGTAG CATTGTAAGC GGCTCTATGA TGAATAACAC ACCATCCACG 
    TACGCGAATA ATAACATCAA TCATCGGAAT TCGTCTGGAG GATCCTTCGA TATCAATTCC 
    GGAACGAATA CGAATACCAA TAATGATTAC ACAAACGGTG CAGGTCCACT GTCGCATCTG 
    AATGAATCAG TCAACTCTTT AGACCCTCTC AACGCTATGG AGAAATCGCT TAACGATCAG 
    ATGCCTCATA CACCACATAC ACCTCACACC CCCCATACGC CCCACACACC AGGTGGTGGA 
    AATAGTGGTC CACCGAGTGT TCCCCCTGCA TCTCAGGAAT CCACGGGAAA TCATAATACA 
    TCTGGTAATA CGAGTACAAA CATAAATAAC GATACTGCAG ACATACCATC TGACTTAAAT 
    TTCGACCCAG CTGCAGTAAT CGATGGCGAG GGTACTGGTC AAGAGGCATT GAATCTTTTG 
    CCAGACAATG TTGTGGATCC AATGGAACTC CTGTCGTATC TGGATCCACC AGATCTGAAC 
    ACCCCTCCCA GTAGCGGCGC TAGCAGTGGT AACCCCTCAT CAAGTGACGA TATATTAGCA 
    CTCTTCGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC100876845 ( XM_012292210.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012147600.1 Megachile rotundata zinc finger MIZ domain-containing protein 1 (LOC100876845), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OMe110724
    Clone ID Related Accession (Same CDS sequence) XM_012292209.1 , XM_012292211.1 , XM_012292208.1 , XM_012292210.1
    Accession Version XM_012292210.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2592bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-04-06
    Organism Megachile rotundata(alfalfa leafcutting bee)
    Product zinc finger MIZ domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797384.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGTGGCAG CTGCCACCGC TACGGCCACC GCCACTGCAA GCGTGGTGGC CATGCAGGAC 
    CGCCAAGACA TCCCCTCGCA ATTCAATCAG ATGCAGAGCC AACATGGAAT CCCCCACCAA 
    ACGTACAACG CAGGGTACGC CCAAAGAGGA CCAATGGCCG GAATGGGGCC AGTCGGGATG 
    AGCAACTTTA ACAGCATGGG CACGATGAGC CCAATGCACT CTATGAATTC CATGAATTCG 
    ATGAACAGTA TGAATGGCAT GAACTCTATG AATCCTATGT CGATGGGAGG TATGAACGGT 
    ATGACTGGTA TGAACGGAAT GACCCCCATG AGCTCCATGA GTAACATGGG TAATATGGGC 
    ATGAACAATA TGATGGGACC TAACAACATG CAGATGAACA AGATGAGCAT GCAGGCTCAA 
    CCACACCAGG GCTATCCAAG AAGACTAGCA CCTTACCCAA ACCCTGCGAT GCACATGGCG 
    CAAAAGAGGC AACAGGGTCC TTACCCGGCT CCCAACCCAG CCGCGATGCA ACCAACCTTC 
    AACGGCATGG CGACGTCGCA GTACCCGACC AATTACCCCG CGGCAGCGAG GCCGAATTTT 
    CAGCCTCAGT ATCAACCGAT GCAGAGCATG AATCCGTCGG CTGCCGGCTT TGGGCCTAAC 
    TCTATGATAC GAGGCGCCAA CATGAGGCAA ACAGCGCCTT CGTATCAGAC CGCGAGTCAG 
    GCGGCAACCA ACCAATACTA CGGTAGCAAT GGCATACCGG TTAGCATGGG ACCTACCACG 
    GTTGGCAATC AATTCGTTGG ACACCAACCG AACACGGGAT ACGCCGGTGC AGCGTCCTCG 
    TACGGGGCAA CCGGGGCTGT CACCAGTCAG TACCAACAGG ACGTCGCGGC GATGAGAACG 
    ACGGGAGCAG GGACACTGAG TTACCAGCAC AGCCCTATCC CTGGGAATCC GACACCTCCG 
    CTCACCCCGG CTACGAGTAT GCCCCCTTAC ATCAGTCCGA ATCCGGATAT CAAGCCTAAT 
    TTCAACGATA TGAAGTCACC GGTTAATATT CAGAAGGACG ATGAGTTAAG ATTAACATTC 
    CCCGTGAGGG ATGGCATCAT TCTTCCACCC TTCCGCTTAG AACATAATCT GGCTGTAAGC 
    AACCACGTAT TCCAACTGAA GTCCACGGTG CATCAAACGT TGATGTGGCG GTCCGACCTG 
    GAACTACAGC TCAAGTGTTT CCACCACGAA GACAGGCAAA TGAACACGAA CTGGCCCGCG 
    AGCGTGCAAG TATCCGTAAA CGCCACGCCG TTGGTTATCG ATCGTGGTGA AAATAAAACG 
    TCTCATAAGC CCTTATACCT GAAGGACGTT TGTCAATCAG GAAGGAACAC AATACAGATC 
    ACGGTGTCGG CGTGTTGTTG TTCCCATTTA TTCGTGCTAC AATTAGTTCA TCGACCAAGC 
    GTGCGAAGCG TGCTTCAGGG ATTGTTACGT AAGAGGCTGC TCACGGCGGA ACATTGTATC 
    ACTAAAATTA AACGAAATTT CAGTAATACT ATTTCCAATA ACGGTATACA GTCGGAGAAG 
    GATGTTGTAG AACAGACAGC ACTTAAGGTA TCCTTAAAAT GTCCTATTAC CTTTAAACGT 
    ATTACACTGC CAGCTAGGGG ACACGATTGT AAACATATTC AGTGCTTCGA TTTGGAGTCG 
    TACCTACAGC TAAATTGTGA AAGAGGATCG TGGAGATGTC CTGTATGCAC GAAACCTGCC 
    CAATTAGAAG GTTTAGAGGT TGACCAGTAC ATGTGGGGCA TACTGAACAC ATTAAACACG 
    GCGGAGGTTG AAGAAGTGAC GATAGACTCG GTCGCGAATT GGAAACCAGC GAAGAATTTA 
    ACTGGTATTA AGTCCGAAGA AGAAAGCGAT TGCAAGAGGA TGACGAAGGC CATGTCACCT 
    GGAAGTATGA ACATGCCAAC GATGAATAAT TGGGATATGA ATCAGGCAAT GAGTCCGTAC 
    ATACCGCCCG ACATGAGTAG CATTGTAAGC GGCTCTATGA TGAATAACAC ACCATCCACG 
    TACGCGAATA ATAACATCAA TCATCGGAAT TCGTCTGGAG GATCCTTCGA TATCAATTCC 
    GGAACGAATA CGAATACCAA TAATGATTAC ACAAACGGTG CAGGTCCACT GTCGCATCTG 
    AATGAATCAG TCAACTCTTT AGACCCTCTC AACGCTATGG AGAAATCGCT TAACGATCAG 
    ATGCCTCATA CACCACATAC ACCTCACACC CCCCATACGC CCCACACACC AGGTGGTGGA 
    AATAGTGGTC CACCGAGTGT TCCCCCTGCA TCTCAGGAAT CCACGGGAAA TCATAATACA 
    TCTGGTAATA CGAGTACAAA CATAAATAAC GATACTGCAG ACATACCATC TGACTTAAAT 
    TTCGACCCAG CTGCAGTAAT CGATGGCGAG GGTACTGGTC AAGAGGCATT GAATCTTTTG 
    CCAGACAATG TTGTGGATCC AATGGAACTC CTGTCGTATC TGGATCCACC AGATCTGAAC 
    ACCCCTCCCA GTAGCGGCGC TAGCAGTGGT AACCCCTCAT CAAGTGACGA TATATTAGCA 
    CTCTTCGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012147600.1
    CDS1039..3630
    Translation

    Target ORF information:

    RefSeq Version XM_012292210.1
    Organism Megachile rotundata(alfalfa leafcutting bee)
    Definition Megachile rotundata zinc finger MIZ domain-containing protein 1 (LOC100876845), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012292210.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGTGGCAG CTGCCACCGC TACGGCCACC GCCACTGCAA GCGTGGTGGC CATGCAGGAC 
    CGCCAAGACA TCCCCTCGCA ATTCAATCAG ATGCAGAGCC AACATGGAAT CCCCCACCAA 
    ACGTACAACG CAGGGTACGC CCAAAGAGGA CCAATGGCCG GAATGGGGCC AGTCGGGATG 
    AGCAACTTTA ACAGCATGGG CACGATGAGC CCAATGCACT CTATGAATTC CATGAATTCG 
    ATGAACAGTA TGAATGGCAT GAACTCTATG AATCCTATGT CGATGGGAGG TATGAACGGT 
    ATGACTGGTA TGAACGGAAT GACCCCCATG AGCTCCATGA GTAACATGGG TAATATGGGC 
    ATGAACAATA TGATGGGACC TAACAACATG CAGATGAACA AGATGAGCAT GCAGGCTCAA 
    CCACACCAGG GCTATCCAAG AAGACTAGCA CCTTACCCAA ACCCTGCGAT GCACATGGCG 
    CAAAAGAGGC AACAGGGTCC TTACCCGGCT CCCAACCCAG CCGCGATGCA ACCAACCTTC 
    AACGGCATGG CGACGTCGCA GTACCCGACC AATTACCCCG CGGCAGCGAG GCCGAATTTT 
    CAGCCTCAGT ATCAACCGAT GCAGAGCATG AATCCGTCGG CTGCCGGCTT TGGGCCTAAC 
    TCTATGATAC GAGGCGCCAA CATGAGGCAA ACAGCGCCTT CGTATCAGAC CGCGAGTCAG 
    GCGGCAACCA ACCAATACTA CGGTAGCAAT GGCATACCGG TTAGCATGGG ACCTACCACG 
    GTTGGCAATC AATTCGTTGG ACACCAACCG AACACGGGAT ACGCCGGTGC AGCGTCCTCG 
    TACGGGGCAA CCGGGGCTGT CACCAGTCAG TACCAACAGG ACGTCGCGGC GATGAGAACG 
    ACGGGAGCAG GGACACTGAG TTACCAGCAC AGCCCTATCC CTGGGAATCC GACACCTCCG 
    CTCACCCCGG CTACGAGTAT GCCCCCTTAC ATCAGTCCGA ATCCGGATAT CAAGCCTAAT 
    TTCAACGATA TGAAGTCACC GGTTAATATT CAGAAGGACG ATGAGTTAAG ATTAACATTC 
    CCCGTGAGGG ATGGCATCAT TCTTCCACCC TTCCGCTTAG AACATAATCT GGCTGTAAGC 
    AACCACGTAT TCCAACTGAA GTCCACGGTG CATCAAACGT TGATGTGGCG GTCCGACCTG 
    GAACTACAGC TCAAGTGTTT CCACCACGAA GACAGGCAAA TGAACACGAA CTGGCCCGCG 
    AGCGTGCAAG TATCCGTAAA CGCCACGCCG TTGGTTATCG ATCGTGGTGA AAATAAAACG 
    TCTCATAAGC CCTTATACCT GAAGGACGTT TGTCAATCAG GAAGGAACAC AATACAGATC 
    ACGGTGTCGG CGTGTTGTTG TTCCCATTTA TTCGTGCTAC AATTAGTTCA TCGACCAAGC 
    GTGCGAAGCG TGCTTCAGGG ATTGTTACGT AAGAGGCTGC TCACGGCGGA ACATTGTATC 
    ACTAAAATTA AACGAAATTT CAGTAATACT ATTTCCAATA ACGGTATACA GTCGGAGAAG 
    GATGTTGTAG AACAGACAGC ACTTAAGGTA TCCTTAAAAT GTCCTATTAC CTTTAAACGT 
    ATTACACTGC CAGCTAGGGG ACACGATTGT AAACATATTC AGTGCTTCGA TTTGGAGTCG 
    TACCTACAGC TAAATTGTGA AAGAGGATCG TGGAGATGTC CTGTATGCAC GAAACCTGCC 
    CAATTAGAAG GTTTAGAGGT TGACCAGTAC ATGTGGGGCA TACTGAACAC ATTAAACACG 
    GCGGAGGTTG AAGAAGTGAC GATAGACTCG GTCGCGAATT GGAAACCAGC GAAGAATTTA 
    ACTGGTATTA AGTCCGAAGA AGAAAGCGAT TGCAAGAGGA TGACGAAGGC CATGTCACCT 
    GGAAGTATGA ACATGCCAAC GATGAATAAT TGGGATATGA ATCAGGCAAT GAGTCCGTAC 
    ATACCGCCCG ACATGAGTAG CATTGTAAGC GGCTCTATGA TGAATAACAC ACCATCCACG 
    TACGCGAATA ATAACATCAA TCATCGGAAT TCGTCTGGAG GATCCTTCGA TATCAATTCC 
    GGAACGAATA CGAATACCAA TAATGATTAC ACAAACGGTG CAGGTCCACT GTCGCATCTG 
    AATGAATCAG TCAACTCTTT AGACCCTCTC AACGCTATGG AGAAATCGCT TAACGATCAG 
    ATGCCTCATA CACCACATAC ACCTCACACC CCCCATACGC CCCACACACC AGGTGGTGGA 
    AATAGTGGTC CACCGAGTGT TCCCCCTGCA TCTCAGGAAT CCACGGGAAA TCATAATACA 
    TCTGGTAATA CGAGTACAAA CATAAATAAC GATACTGCAG ACATACCATC TGACTTAAAT 
    TTCGACCCAG CTGCAGTAAT CGATGGCGAG GGTACTGGTC AAGAGGCATT GAATCTTTTG 
    CCAGACAATG TTGTGGATCC AATGGAACTC CTGTCGTATC TGGATCCACC AGATCTGAAC 
    ACCCCTCCCA GTAGCGGCGC TAGCAGTGGT AACCCCTCAT CAAGTGACGA TATATTAGCA 
    CTCTTCGAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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