Grk5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Grk5
Entrez Gene ID	14773
Full Name	G protein-coupled receptor kinase 5
Synonyms	Gprk5
General protein information	Preferred Names G protein-coupled receptor kinase 5 Names G protein-coupled receptor kinase 5 g protein-coupled receptor kinase GRK5
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19 D3\|19 56.52 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_011247149.3	XP_011245451.1	G protein-coupled receptor kinase 5 isoform X2
XM_017318066.1	XP_017173555.1	G protein-coupled receptor kinase 5 isoform X3
NM_018869.3	NP_061357.3	G protein-coupled receptor kinase 5
XM_006526697.4	XP_006526760.1	G protein-coupled receptor kinase 5 isoform X1
XM_006526697.3	XP_006526760.1	G protein-coupled receptor kinase 5 isoform X1
XM_011247149.2	XP_011245451.1	G protein-coupled receptor kinase 5 isoform X2
XM_017318066.2	XP_017173555.1	G protein-coupled receptor kinase 5 isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GRK5	XP_003312835.1
Bos taurus (cattle)	GRK5	NP_776756.1
Xenopus tropicalis (tropical clawed frog)	LOC100497344	XP_002940394.1
	LOC100427251	XP_002805891.1
Mus musculus (house mouse)	Grk5	NP_061357.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004117	XP_312995.5
Caenorhabditis elegans (roundworm)	grk-1	NP_509676.1
Homo sapiens (human)	GRK5	NP_005299.1
Canis lupus familiaris (dog)	GRK5	XP_544045.3
Rattus norvegicus (Norway rat)	Grk5	NP_110456.1
Gallus gallus (chicken)	GRK5	XP_421789.2
Drosophila melanogaster (fruit fly)	Gprk2	NP_476867.1

Gene Ontology
Bibliography

Related articles in PubMed

GRK5 Regulates Social Behavior Via Suppression of mTORC1 Signaling in Medial Prefrontal Cortex.
Niu B, Liu P, Shen M, Liu C, Wang L, Wang F, Ma L
Cerebral cortex (New York, N.Y. : 1991)28(2)421-432(2018 Feb)

GRK5-Knockout Mice Generated by TALEN-Mediated Gene Targeting.
Nanjidsuren T, Park CW, Sim BW, Kim SU, Chang KT, Kang MH, Min KS
Animal biotechnology27(4)223-30(2016 Oct)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

GRK5 Deficiency Leads to Selective Basal Forebrain Cholinergic Neuronal Vulnerability.
He M, Singh P, Cheng S, Zhang Q, Peng W, Ding X, Li L, Liu J, Premont RT, Morgan D, Burns JM, Swerdlow RH, Suo WZ
Scientific reports626116(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Generated mice with genetic knockout (KO) of the G protein-coupled receptor kinase (GRK) 5 gene by microinjection of TALEN mRNA. TALEN vectors were designed to target exons 1, 3, and 5 of the mouse GRK5 gene.
Title: GRK5-Knockout Mice Generated by TALEN-Mediated Gene Targeting.

Overall, these data suggest that GRK5 negatively regulates CXCL1/KC levels during bacterial pneumonia but that the role of GRK5 in the clinical outcome in this model is dependent on the bacterial dose.
Title: Bacterial Dose-Dependent Role of G Protein-Coupled Receptor Kinase 5 in Escherichia coli-Induced Pneumonia.

These results of this study suggest that GRK5 deficiency indeed makes the mice more susceptible to wide range of behavioral impairments, including cognitive impairments.
Title: GRK5 deficiency leads to susceptibility to intermittent hypoxia-induced cognitive impairment.

GRK5, acting in a kinase independent manner, is a facilitator of NFAT activity and part of a DNA-binding complex responsible for pathological hypertrophic gene transcription.
Title: GRK5-mediated exacerbation of pathological cardiac hypertrophy involves facilitation of nuclear NFAT activity.

MOR phosphorylation is regulated by agonist-selective recruitment of distinct GRK isoforms that influence different opioid-related behaviors. Modulation of GRK5 function could serve as a new approach for preventing addiction to opioids.
Title: Loss of morphine reward and dependence in mice lacking G protein-coupled receptor kinase 5.

The following Grk5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Grk5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu14882	NM_018869.3 Latest version!	Mus musculus G protein-coupled receptor kinase 5 (Grk5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$349.30 $499.00
OMu55262	XM_006526697.4 Latest version!	Mus musculus G protein-coupled receptor kinase 5 (Grk5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu67848	XM_011247149.3 Latest version!	Mus musculus G protein-coupled receptor kinase 5 (Grk5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu79888	XM_017318066.2 Latest version!	Mus musculus G protein-coupled receptor kinase 5 (Grk5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu55262	XM_006526697.3	Mus musculus G protein-coupled receptor kinase 5 (Grk5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu67848	XM_011247149.2	Mus musculus G protein-coupled receptor kinase 5 (Grk5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu79888	XM_017318066.1	Mus musculus G protein-coupled receptor kinase 5 (Grk5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Grk5 ( NM_018869.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_061357.3 Mus musculus G protein-coupled receptor kinase 5 (Grk5), mRNA.

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CloneID	OMu14882
Clone ID Related Accession (Same CDS sequence)	NM_018869.3
Accession Version	NM_018869.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-15
Organism	Mus musculus(house mouse)
Product	G protein-coupled receptor kinase 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK135076.1, BY289760.1, AK161821.1 and AW456000.1. On Nov 2, 2007 this sequence version replaced NM_018869.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK135076.1, AK161821.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTGG AAAACATCGT GGCCAACACG GTCTTGCTGA AAGCCCGGGA AGGGGGTGGA 
GGAAAGCGCA AAGGGAAAAG CAAGAAGTGG AAGGAAATCC TGAAGTTTCC TCACATCAGC 
CAGTGTGAAG ACCTCCGAAG GACCATAGAC AGAGATTACT ACAGTCTATG TGACAAGCAA 
CCAATTGGGA GACTGCTTTT TCGACAGTTC TGTGAAACCA GGCCTGGGCT GGAGTGCTAC 
ATTCAGTTCC TGGACTTAGT GGCAGAATAT GAAATTACTC CAGATGAAAA CCTTGGGGCG 
AAGGGGAAGG AAATAATGAC CAAGTACCTC ACTCCAAAGT CCCCAGTCTT CATTGCCCAA 
GTTGGACAGG ACCTGGTCTC CCAGACAGAG AAGAAGCTCC TGCAGAGCCC CTGCAAAGAA 
CTCTTCTCTG CTTGTGCTCA GTCTGTCCAT GACTACTTGA AGGGAGACCC CTTCCACGAG 
TACCTGGATA GCATGTATTT TGACCGTTTT CTGCAGTGGA AATGGTTAGA AAGACAACCA 
GTGACCAAAA ACACTTTCCG GCAGTACCGA GTGCTGGGCA AAGGGGGCTT TGGAGAGGTC 
TGTGCCTGCC AGGTTCGGGC CACTGGTAAA ATGTATGCTT GTAAACGCTT AGAGAAGAAG 
AGGATCAAAA AGAGGAAAGG CGAATCCATG GCACTCAACG AAAAGCAGAT TCTTGAGAAG 
GTCAACAGCC AGTTTGTGGT CAACCTGGCC TATGCCTATG AAACCAAAGA TGCACTATGC 
CTGGTTCTGA CCATTATGAA TGGTGGTGAC CTGAAGTTTC ACATCTACAA TATGGGGAAT 
CCTGGCTTTG AGGAAGAGCG AGCCTTATTT TATGCAGCTG AGATCCTCTG TGGCCTAGAA 
GACTTACACC GTGAGAACAC TGTCTATAGA GATCTAAAAC CCGAAAACAT CTTGCTGGAT 
GATTATGGCC ACATAAGGAT CTCAGACCTC GGACTGGCCG TGAAGATCCC CGAGGGAGAC 
CTTATCCGTG GCCGGGTAGG CACTGTTGGC TACATGGCCC CAGAAGTTCT GAACAACCAG 
CGATATGGAC TGAGCCCTGA CTACTGGGGC CTGGGCTGCC TCATCTATGA GATGATTGAA 
GGCCAGTCAC CATTTCGAGG TCGCAAGGAG AAGGTTAAGC GGGAAGAGGT GGATCGCCGG 
GTGCTGGAGA CTGAGGAAGT GTATTCCTCC AAGTTCTCTG AAGAGGCCAA GTCCATCTGC 
AACATGCTGC TCACCAAAGA CTCGAAGCAG AGGCTGGGCT GCCAGGAGGA GGGGGCCGCC 
GAGGTCAAGA GGCACCCTTT CTTCAGGAAC ATGAACTTTA AGCGCCTGGA GGCTGGGATG 
TTGGACCCTC CCTTCGTTCC AGATCCCCGG GCTGTATACT GCAAGGATGT GCTGGACATT 
GAGCAGTTCT CCACTGTGAA AGGTGTCAAC CTGGACCATA CGGACGATGA TTTTTACTCA 
AAGTTCTCTA CAGGCTCTGT GCCAATTCCA TGGCAAAATG AGATGATAGA AACAGAATGT 
TTCAAGGAGC TGAATGTGTT CGGACCTAAC GGTACCCTCT CACCAGACCT GAACAGAAGT 
CAGCCTCCAG AACCGCCAAA GAAAGGGCTG TTCCACAGAC TCTTCAGGCG TCAGCATCAA 
AGCAATTCCA AGAGTTCACC TACTCCTAAG ACCAGTTGTA ACCACCGAAT AAATTCAAAC 
CACATCAATT CAAACTCCAC TGGAAGCAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061357.3
CDS	832..2604
Translation	MELENIVANTVLLKAREGGGGKRKGKSKKWKEILKFPHISQCEDLRRTIDRDYYSLCDKQPIGRLLFRQFCETRPGLECYIQFLDLVAEYEITPDENLGAKGKEIMTKYLTPKSPVFIAQVGQDLVSQTEKKLLQSPCKELFSACAQSVHDYLKGDPFHEYLDSMYFDRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKRLEKKRIKKRKGESMALNEKQILEKVNSQFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNPGFEEERALFYAAEILCGLEDLHRENTVYRDLKPENILLDDYGHIRISDLGLAVKIPEGDLIRGRVGTVGYMAPEVLNNQRYGLSPDYWGLGCLIYEMIEGQSPFRGRKEKVKREEVDRRVLETEEVYSSKFSEEAKSICNMLLTKDSKQRLGCQEEGAAEVKRHPFFRNMNFKRLEAGMLDPPFVPDPRAVYCKDVLDIEQFSTVKGVNLDHTDDDFYSKFSTGSVPIPWQNEMIETECFKELNVFGPNGTLSPDLNRSQPPEPPKKGLFHRLFRRQHQSNSKSSPTPKTSCNHRINSNHINSNSTGSS

Target ORF information:

RefSeq Version	NM_018869.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus G protein-coupled receptor kinase 5 (Grk5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018869.3

ORF Insert Sequence:

ATGGAGCTGG AAAACATCGT GGCCAACACG GTCTTGCTGA AAGCCCGGGA AGGGGGTGGA 
GGAAAGCGCA AAGGGAAAAG CAAGAAGTGG AAGGAAATCC TGAAGTTTCC TCACATCAGC 
CAGTGTGAAG ACCTCCGAAG GACCATAGAC AGAGATTACT ACAGTCTATG TGACAAGCAA 
CCAATTGGGA GACTGCTTTT TCGACAGTTC TGTGAAACCA GGCCTGGGCT GGAGTGCTAC 
ATTCAGTTCC TGGACTTAGT GGCAGAATAT GAAATTACTC CAGATGAAAA CCTTGGGGCG 
AAGGGGAAGG AAATAATGAC CAAGTACCTC ACTCCAAAGT CCCCAGTCTT CATTGCCCAA 
GTTGGACAGG ACCTGGTCTC CCAGACAGAG AAGAAGCTCC TGCAGAGCCC CTGCAAAGAA 
CTCTTCTCTG CTTGTGCTCA GTCTGTCCAT GACTACTTGA AGGGAGACCC CTTCCACGAG 
TACCTGGATA GCATGTATTT TGACCGTTTT CTGCAGTGGA AATGGTTAGA AAGACAACCA 
GTGACCAAAA ACACTTTCCG GCAGTACCGA GTGCTGGGCA AAGGGGGCTT TGGAGAGGTC 
TGTGCCTGCC AGGTTCGGGC CACTGGTAAA ATGTATGCTT GTAAACGCTT AGAGAAGAAG 
AGGATCAAAA AGAGGAAAGG CGAATCCATG GCACTCAACG AAAAGCAGAT TCTTGAGAAG 
GTCAACAGCC AGTTTGTGGT CAACCTGGCC TATGCCTATG AAACCAAAGA TGCACTATGC 
CTGGTTCTGA CCATTATGAA TGGTGGTGAC CTGAAGTTTC ACATCTACAA TATGGGGAAT 
CCTGGCTTTG AGGAAGAGCG AGCCTTATTT TATGCAGCTG AGATCCTCTG TGGCCTAGAA 
GACTTACACC GTGAGAACAC TGTCTATAGA GATCTAAAAC CCGAAAACAT CTTGCTGGAT 
GATTATGGCC ACATAAGGAT CTCAGACCTC GGACTGGCCG TGAAGATCCC CGAGGGAGAC 
CTTATCCGTG GCCGGGTAGG CACTGTTGGC TACATGGCCC CAGAAGTTCT GAACAACCAG 
CGATATGGAC TGAGCCCTGA CTACTGGGGC CTGGGCTGCC TCATCTATGA GATGATTGAA 
GGCCAGTCAC CATTTCGAGG TCGCAAGGAG AAGGTTAAGC GGGAAGAGGT GGATCGCCGG 
GTGCTGGAGA CTGAGGAAGT GTATTCCTCC AAGTTCTCTG AAGAGGCCAA GTCCATCTGC 
AACATGCTGC TCACCAAAGA CTCGAAGCAG AGGCTGGGCT GCCAGGAGGA GGGGGCCGCC 
GAGGTCAAGA GGCACCCTTT CTTCAGGAAC ATGAACTTTA AGCGCCTGGA GGCTGGGATG 
TTGGACCCTC CCTTCGTTCC AGATCCCCGG GCTGTATACT GCAAGGATGT GCTGGACATT 
GAGCAGTTCT CCACTGTGAA AGGTGTCAAC CTGGACCATA CGGACGATGA TTTTTACTCA 
AAGTTCTCTA CAGGCTCTGT GCCAATTCCA TGGCAAAATG AGATGATAGA AACAGAATGT 
TTCAAGGAGC TGAATGTGTT CGGACCTAAC GGTACCCTCT CACCAGACCT GAACAGAAGT 
CAGCCTCCAG AACCGCCAAA GAAAGGGCTG TTCCACAGAC TCTTCAGGCG TCAGCATCAA 
AGCAATTCCA AGAGTTCACC TACTCCTAAG ACCAGTTGTA ACCACCGAAT AAATTCAAAC 
CACATCAATT CAAACTCCAC TGGAAGCAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grk5 ( XM_006526697.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526760.1 Mus musculus G protein-coupled receptor kinase 5 (Grk5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu55262
Clone ID Related Accession (Same CDS sequence)	XM_006526697.4 , XM_006526697.3
Accession Version	XM_006526697.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	G protein-coupled receptor kinase 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006526697.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG AAAACATCGT GGCCAACACG GTCTTGCTGA AAGCCCGGGA AGACAGAGAT 
TACTACAGTC TATGTGACAA GCAACCAATT GGGAGACTGC TTTTTCGACA GTTCTGTGAA 
ACCAGGCCTG GGCTGGAGTG CTACATTCAG TTCCTGGACT TAGTGGCAGA ATATGAAATT 
ACTCCAGATG AAAACCTTGG GGCGAAGGGG AAGGAAATAA TGACCAAGTA CCTCACTCCA 
AAGTCCCCAG TCTTCATTGC CCAAGTTGGA CAGGACCTGG TCTCCCAGAC AGAGAAGAAG 
CTCCTGCAGA GCCCCTGCAA AGAACTCTTC TCTGCTTGTG CTCAGTCTGT CCATGACTAC 
TTGAAGGGAG ACCCCTTCCA CGAGTACCTG GATAGCATGT ATTTTGACCG TTTTCTGCAG 
TGGAAATGGT TAGAAAGACA ACCAGTGACC AAAAACACTT TCCGGCAGTA CCGAGTGCTG 
GGCAAAGGGG GCTTTGGAGA GGTCTGTGCC TGCCAGGTTC GGGCCACTGG TAAAATGTAT 
GCTTGTAAAC GCTTAGAGAA GAAGAGGATC AAAAAGAGGA AAGGCGAATC CATGGCACTC 
AACGAAAAGC AGATTCTTGA GAAGGTCAAC AGCCAGTTTG TGGTCAACCT GGCCTATGCC 
TATGAAACCA AAGATGCACT ATGCCTGGTT CTGACCATTA TGAATGGTGG TGACCTGAAG 
TTTCACATCT ACAATATGGG GAATCCTGGC TTTGAGGAAG AGCGAGCCTT ATTTTATGCA 
GCTGAGATCC TCTGTGGCCT AGAAGACTTA CACCGTGAGA ACACTGTCTA TAGAGATCTA 
AAACCCGAAA ACATCTTGCT GGATGATTAT GGCCACATAA GGATCTCAGA CCTCGGACTG 
GCCGTGAAGA TCCCCGAGGG AGACCTTATC CGTGGCCGGG TAGGCACTGT TGGCTACATG 
GCCCCAGAAG TTCTGAACAA CCAGCGATAT GGACTGAGCC CTGACTACTG GGGCCTGGGC 
TGCCTCATCT ATGAGATGAT TGAAGGCCAG TCACCATTTC GAGGTCGCAA GGAGAAGGTT 
AAGCGGGAAG AGGTGGATCG CCGGGTGCTG GAGACTGAGG AAGTGTATTC CTCCAAGTTC 
TCTGAAGAGG CCAAGTCCAT CTGCAACATG CTGCTCACCA AAGACTCGAA GCAGAGGCTG 
GGCTGCCAGG AGGAGGGGGC CGCCGAGGTC AAGAGGCACC CTTTCTTCAG GAACATGAAC 
TTTAAGCGCC TGGAGGCTGG GATGTTGGAC CCTCCCTTCG TTCCAGATCC CCGGGCTGTA 
TACTGCAAGG ATGTGCTGGA CATTGAGCAG TTCTCCACTG TGAAAGGTGT CAACCTGGAC 
CATACGGACG ATGATTTTTA CTCAAAGTTC TCTACAGGCT CTGTGCCAAT TCCATGGCAA 
AATGAGATGA TAGAAACAGA ATGTTTCAAG GAGCTGAATG TGTTCGGACC TAACGGTACC 
CTCTCACCAG ACCTGAACAG AAGTCAGCCT CCAGAACCGC CAAAGAAAGG GCTGTTCCAC 
AGACTCTTCA GGCGTCAGCA TCAAAGCAAT TCCAAGAGTT CACCTACTCC TAAGACCAGT 
TGTAACCACC GAATAAATTC AAACCACATC AATTCAAACT CCACTGGAAG CAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526760.1
CDS	971..2647
Translation	MELENIVANTVLLKAREDRDYYSLCDKQPIGRLLFRQFCETRPGLECYIQFLDLVAEYEITPDENLGAKGKEIMTKYLTPKSPVFIAQVGQDLVSQTEKKLLQSPCKELFSACAQSVHDYLKGDPFHEYLDSMYFDRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKRLEKKRIKKRKGESMALNEKQILEKVNSQFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNPGFEEERALFYAAEILCGLEDLHRENTVYRDLKPENILLDDYGHIRISDLGLAVKIPEGDLIRGRVGTVGYMAPEVLNNQRYGLSPDYWGLGCLIYEMIEGQSPFRGRKEKVKREEVDRRVLETEEVYSSKFSEEAKSICNMLLTKDSKQRLGCQEEGAAEVKRHPFFRNMNFKRLEAGMLDPPFVPDPRAVYCKDVLDIEQFSTVKGVNLDHTDDDFYSKFSTGSVPIPWQNEMIETECFKELNVFGPNGTLSPDLNRSQPPEPPKKGLFHRLFRRQHQSNSKSSPTPKTSCNHRINSNHINSNSTGSS

Target ORF information:

RefSeq Version	XM_006526697.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus G protein-coupled receptor kinase 5 (Grk5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526697.3

ORF Insert Sequence:

ATGGAGCTGG AAAACATCGT GGCCAACACG GTCTTGCTGA AAGCCCGGGA AGACAGAGAT 
TACTACAGTC TATGTGACAA GCAACCAATT GGGAGACTGC TTTTTCGACA GTTCTGTGAA 
ACCAGGCCTG GGCTGGAGTG CTACATTCAG TTCCTGGACT TAGTGGCAGA ATATGAAATT 
ACTCCAGATG AAAACCTTGG GGCGAAGGGG AAGGAAATAA TGACCAAGTA CCTCACTCCA 
AAGTCCCCAG TCTTCATTGC CCAAGTTGGA CAGGACCTGG TCTCCCAGAC AGAGAAGAAG 
CTCCTGCAGA GCCCCTGCAA AGAACTCTTC TCTGCTTGTG CTCAGTCTGT CCATGACTAC 
TTGAAGGGAG ACCCCTTCCA CGAGTACCTG GATAGCATGT ATTTTGACCG TTTTCTGCAG 
TGGAAATGGT TAGAAAGACA ACCAGTGACC AAAAACACTT TCCGGCAGTA CCGAGTGCTG 
GGCAAAGGGG GCTTTGGAGA GGTCTGTGCC TGCCAGGTTC GGGCCACTGG TAAAATGTAT 
GCTTGTAAAC GCTTAGAGAA GAAGAGGATC AAAAAGAGGA AAGGCGAATC CATGGCACTC 
AACGAAAAGC AGATTCTTGA GAAGGTCAAC AGCCAGTTTG TGGTCAACCT GGCCTATGCC 
TATGAAACCA AAGATGCACT ATGCCTGGTT CTGACCATTA TGAATGGTGG TGACCTGAAG 
TTTCACATCT ACAATATGGG GAATCCTGGC TTTGAGGAAG AGCGAGCCTT ATTTTATGCA 
GCTGAGATCC TCTGTGGCCT AGAAGACTTA CACCGTGAGA ACACTGTCTA TAGAGATCTA 
AAACCCGAAA ACATCTTGCT GGATGATTAT GGCCACATAA GGATCTCAGA CCTCGGACTG 
GCCGTGAAGA TCCCCGAGGG AGACCTTATC CGTGGCCGGG TAGGCACTGT TGGCTACATG 
GCCCCAGAAG TTCTGAACAA CCAGCGATAT GGACTGAGCC CTGACTACTG GGGCCTGGGC 
TGCCTCATCT ATGAGATGAT TGAAGGCCAG TCACCATTTC GAGGTCGCAA GGAGAAGGTT 
AAGCGGGAAG AGGTGGATCG CCGGGTGCTG GAGACTGAGG AAGTGTATTC CTCCAAGTTC 
TCTGAAGAGG CCAAGTCCAT CTGCAACATG CTGCTCACCA AAGACTCGAA GCAGAGGCTG 
GGCTGCCAGG AGGAGGGGGC CGCCGAGGTC AAGAGGCACC CTTTCTTCAG GAACATGAAC 
TTTAAGCGCC TGGAGGCTGG GATGTTGGAC CCTCCCTTCG TTCCAGATCC CCGGGCTGTA 
TACTGCAAGG ATGTGCTGGA CATTGAGCAG TTCTCCACTG TGAAAGGTGT CAACCTGGAC 
CATACGGACG ATGATTTTTA CTCAAAGTTC TCTACAGGCT CTGTGCCAAT TCCATGGCAA 
AATGAGATGA TAGAAACAGA ATGTTTCAAG GAGCTGAATG TGTTCGGACC TAACGGTACC 
CTCTCACCAG ACCTGAACAG AAGTCAGCCT CCAGAACCGC CAAAGAAAGG GCTGTTCCAC 
AGACTCTTCA GGCGTCAGCA TCAAAGCAAT TCCAAGAGTT CACCTACTCC TAAGACCAGT 
TGTAACCACC GAATAAATTC AAACCACATC AATTCAAACT CCACTGGAAG CAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grk5 ( XM_011247149.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245451.1 Mus musculus G protein-coupled receptor kinase 5 (Grk5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu67848
Clone ID Related Accession (Same CDS sequence)	XM_011247149.3 , XM_011247149.2
Accession Version	XM_011247149.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	G protein-coupled receptor kinase 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247149.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGACA GAGATTACTA CAGTCTATGT GACAAGCAAC CAATTGGGAG ACTGCTTTTT 
CGACAGTTCT GTGAAACCAG GCCTGGGCTG GAGTGCTACA TTCAGTTCCT GGACTTAGTG 
GCAGAATATG AAATTACTCC AGATGAAAAC CTTGGGGCGA AGGGGAAGGA AATAATGACC 
AAGTACCTCA CTCCAAAGTC CCCAGTCTTC ATTGCCCAAG TTGGACAGGA CCTGGTCTCC 
CAGACAGAGA AGAAGCTCCT GCAGAGCCCC TGCAAAGAAC TCTTCTCTGC TTGTGCTCAG 
TCTGTCCATG ACTACTTGAA GGGAGACCCC TTCCACGAGT ACCTGGATAG CATGTATTTT 
GACCGTTTTC TGCAGTGGAA ATGGTTAGAA AGACAACCAG TGACCAAAAA CACTTTCCGG 
CAGTACCGAG TGCTGGGCAA AGGGGGCTTT GGAGAGGTCT GTGCCTGCCA GGTTCGGGCC 
ACTGGTAAAA TGTATGCTTG TAAACGCTTA GAGAAGAAGA GGATCAAAAA GAGGAAAGGC 
GAATCCATGG CACTCAACGA AAAGCAGATT CTTGAGAAGG TCAACAGCCA GTTTGTGGTC 
AACCTGGCCT ATGCCTATGA AACCAAAGAT GCACTATGCC TGGTTCTGAC CATTATGAAT 
GGTGGTGACC TGAAGTTTCA CATCTACAAT ATGGGGAATC CTGGCTTTGA GGAAGAGCGA 
GCCTTATTTT ATGCAGCTGA GATCCTCTGT GGCCTAGAAG ACTTACACCG TGAGAACACT 
GTCTATAGAG ATCTAAAACC CGAAAACATC TTGCTGGATG ATTATGGCCA CATAAGGATC 
TCAGACCTCG GACTGGCCGT GAAGATCCCC GAGGGAGACC TTATCCGTGG CCGGGTAGGC 
ACTGTTGGCT ACATGGCCCC AGAAGTTCTG AACAACCAGC GATATGGACT GAGCCCTGAC 
TACTGGGGCC TGGGCTGCCT CATCTATGAG ATGATTGAAG GCCAGTCACC ATTTCGAGGT 
CGCAAGGAGA AGGTTAAGCG GGAAGAGGTG GATCGCCGGG TGCTGGAGAC TGAGGAAGTG 
TATTCCTCCA AGTTCTCTGA AGAGGCCAAG TCCATCTGCA ACATGCTGCT CACCAAAGAC 
TCGAAGCAGA GGCTGGGCTG CCAGGAGGAG GGGGCCGCCG AGGTCAAGAG GCACCCTTTC 
TTCAGGAACA TGAACTTTAA GCGCCTGGAG GCTGGGATGT TGGACCCTCC CTTCGTTCCA 
GATCCCCGGG CTGTATACTG CAAGGATGTG CTGGACATTG AGCAGTTCTC CACTGTGAAA 
GGTGTCAACC TGGACCATAC GGACGATGAT TTTTACTCAA AGTTCTCTAC AGGCTCTGTG 
CCAATTCCAT GGCAAAATGA GATGATAGAA ACAGAATGTT TCAAGGAGCT GAATGTGTTC 
GGACCTAACG GTACCCTCTC ACCAGACCTG AACAGAAGTC AGCCTCCAGA ACCGCCAAAG 
AAAGGGCTGT TCCACAGACT CTTCAGGCGT CAGCATCAAA GCAATTCCAA GAGTTCACCT 
ACTCCTAAGA CCAGTTGTAA CCACCGAATA AATTCAAACC ACATCAATTC AAACTCCACT 
GGAAGCAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245451.1
CDS	39..1670
Translation	MTDRDYYSLCDKQPIGRLLFRQFCETRPGLECYIQFLDLVAEYEITPDENLGAKGKEIMTKYLTPKSPVFIAQVGQDLVSQTEKKLLQSPCKELFSACAQSVHDYLKGDPFHEYLDSMYFDRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKRLEKKRIKKRKGESMALNEKQILEKVNSQFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNPGFEEERALFYAAEILCGLEDLHRENTVYRDLKPENILLDDYGHIRISDLGLAVKIPEGDLIRGRVGTVGYMAPEVLNNQRYGLSPDYWGLGCLIYEMIEGQSPFRGRKEKVKREEVDRRVLETEEVYSSKFSEEAKSICNMLLTKDSKQRLGCQEEGAAEVKRHPFFRNMNFKRLEAGMLDPPFVPDPRAVYCKDVLDIEQFSTVKGVNLDHTDDDFYSKFSTGSVPIPWQNEMIETECFKELNVFGPNGTLSPDLNRSQPPEPPKKGLFHRLFRRQHQSNSKSSPTPKTSCNHRINSNHINSNSTGSS

Target ORF information:

RefSeq Version	XM_011247149.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus G protein-coupled receptor kinase 5 (Grk5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247149.2

ORF Insert Sequence:

ATGACAGACA GAGATTACTA CAGTCTATGT GACAAGCAAC CAATTGGGAG ACTGCTTTTT 
CGACAGTTCT GTGAAACCAG GCCTGGGCTG GAGTGCTACA TTCAGTTCCT GGACTTAGTG 
GCAGAATATG AAATTACTCC AGATGAAAAC CTTGGGGCGA AGGGGAAGGA AATAATGACC 
AAGTACCTCA CTCCAAAGTC CCCAGTCTTC ATTGCCCAAG TTGGACAGGA CCTGGTCTCC 
CAGACAGAGA AGAAGCTCCT GCAGAGCCCC TGCAAAGAAC TCTTCTCTGC TTGTGCTCAG 
TCTGTCCATG ACTACTTGAA GGGAGACCCC TTCCACGAGT ACCTGGATAG CATGTATTTT 
GACCGTTTTC TGCAGTGGAA ATGGTTAGAA AGACAACCAG TGACCAAAAA CACTTTCCGG 
CAGTACCGAG TGCTGGGCAA AGGGGGCTTT GGAGAGGTCT GTGCCTGCCA GGTTCGGGCC 
ACTGGTAAAA TGTATGCTTG TAAACGCTTA GAGAAGAAGA GGATCAAAAA GAGGAAAGGC 
GAATCCATGG CACTCAACGA AAAGCAGATT CTTGAGAAGG TCAACAGCCA GTTTGTGGTC 
AACCTGGCCT ATGCCTATGA AACCAAAGAT GCACTATGCC TGGTTCTGAC CATTATGAAT 
GGTGGTGACC TGAAGTTTCA CATCTACAAT ATGGGGAATC CTGGCTTTGA GGAAGAGCGA 
GCCTTATTTT ATGCAGCTGA GATCCTCTGT GGCCTAGAAG ACTTACACCG TGAGAACACT 
GTCTATAGAG ATCTAAAACC CGAAAACATC TTGCTGGATG ATTATGGCCA CATAAGGATC 
TCAGACCTCG GACTGGCCGT GAAGATCCCC GAGGGAGACC TTATCCGTGG CCGGGTAGGC 
ACTGTTGGCT ACATGGCCCC AGAAGTTCTG AACAACCAGC GATATGGACT GAGCCCTGAC 
TACTGGGGCC TGGGCTGCCT CATCTATGAG ATGATTGAAG GCCAGTCACC ATTTCGAGGT 
CGCAAGGAGA AGGTTAAGCG GGAAGAGGTG GATCGCCGGG TGCTGGAGAC TGAGGAAGTG 
TATTCCTCCA AGTTCTCTGA AGAGGCCAAG TCCATCTGCA ACATGCTGCT CACCAAAGAC 
TCGAAGCAGA GGCTGGGCTG CCAGGAGGAG GGGGCCGCCG AGGTCAAGAG GCACCCTTTC 
TTCAGGAACA TGAACTTTAA GCGCCTGGAG GCTGGGATGT TGGACCCTCC CTTCGTTCCA 
GATCCCCGGG CTGTATACTG CAAGGATGTG CTGGACATTG AGCAGTTCTC CACTGTGAAA 
GGTGTCAACC TGGACCATAC GGACGATGAT TTTTACTCAA AGTTCTCTAC AGGCTCTGTG 
CCAATTCCAT GGCAAAATGA GATGATAGAA ACAGAATGTT TCAAGGAGCT GAATGTGTTC 
GGACCTAACG GTACCCTCTC ACCAGACCTG AACAGAAGTC AGCCTCCAGA ACCGCCAAAG 
AAAGGGCTGT TCCACAGACT CTTCAGGCGT CAGCATCAAA GCAATTCCAA GAGTTCACCT 
ACTCCTAAGA CCAGTTGTAA CCACCGAATA AATTCAAACC ACATCAATTC AAACTCCACT 
GGAAGCAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grk5 ( XM_006526697.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006526760.1 Mus musculus G protein-coupled receptor kinase 5 (Grk5), transcript variant X1, mRNA.

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CloneID	OMu55262
Clone ID Related Accession (Same CDS sequence)	XM_006526697.4 , XM_006526697.3
Accession Version	XM_006526697.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	G protein-coupled receptor kinase 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006526697.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG AAAACATCGT GGCCAACACG GTCTTGCTGA AAGCCCGGGA AGACAGAGAT 
TACTACAGTC TATGTGACAA GCAACCAATT GGGAGACTGC TTTTTCGACA GTTCTGTGAA 
ACCAGGCCTG GGCTGGAGTG CTACATTCAG TTCCTGGACT TAGTGGCAGA ATATGAAATT 
ACTCCAGATG AAAACCTTGG GGCGAAGGGG AAGGAAATAA TGACCAAGTA CCTCACTCCA 
AAGTCCCCAG TCTTCATTGC CCAAGTTGGA CAGGACCTGG TCTCCCAGAC AGAGAAGAAG 
CTCCTGCAGA GCCCCTGCAA AGAACTCTTC TCTGCTTGTG CTCAGTCTGT CCATGACTAC 
TTGAAGGGAG ACCCCTTCCA CGAGTACCTG GATAGCATGT ATTTTGACCG TTTTCTGCAG 
TGGAAATGGT TAGAAAGACA ACCAGTGACC AAAAACACTT TCCGGCAGTA CCGAGTGCTG 
GGCAAAGGGG GCTTTGGAGA GGTCTGTGCC TGCCAGGTTC GGGCCACTGG TAAAATGTAT 
GCTTGTAAAC GCTTAGAGAA GAAGAGGATC AAAAAGAGGA AAGGCGAATC CATGGCACTC 
AACGAAAAGC AGATTCTTGA GAAGGTCAAC AGCCAGTTTG TGGTCAACCT GGCCTATGCC 
TATGAAACCA AAGATGCACT ATGCCTGGTT CTGACCATTA TGAATGGTGG TGACCTGAAG 
TTTCACATCT ACAATATGGG GAATCCTGGC TTTGAGGAAG AGCGAGCCTT ATTTTATGCA 
GCTGAGATCC TCTGTGGCCT AGAAGACTTA CACCGTGAGA ACACTGTCTA TAGAGATCTA 
AAACCCGAAA ACATCTTGCT GGATGATTAT GGCCACATAA GGATCTCAGA CCTCGGACTG 
GCCGTGAAGA TCCCCGAGGG AGACCTTATC CGTGGCCGGG TAGGCACTGT TGGCTACATG 
GCCCCAGAAG TTCTGAACAA CCAGCGATAT GGACTGAGCC CTGACTACTG GGGCCTGGGC 
TGCCTCATCT ATGAGATGAT TGAAGGCCAG TCACCATTTC GAGGTCGCAA GGAGAAGGTT 
AAGCGGGAAG AGGTGGATCG CCGGGTGCTG GAGACTGAGG AAGTGTATTC CTCCAAGTTC 
TCTGAAGAGG CCAAGTCCAT CTGCAACATG CTGCTCACCA AAGACTCGAA GCAGAGGCTG 
GGCTGCCAGG AGGAGGGGGC CGCCGAGGTC AAGAGGCACC CTTTCTTCAG GAACATGAAC 
TTTAAGCGCC TGGAGGCTGG GATGTTGGAC CCTCCCTTCG TTCCAGATCC CCGGGCTGTA 
TACTGCAAGG ATGTGCTGGA CATTGAGCAG TTCTCCACTG TGAAAGGTGT CAACCTGGAC 
CATACGGACG ATGATTTTTA CTCAAAGTTC TCTACAGGCT CTGTGCCAAT TCCATGGCAA 
AATGAGATGA TAGAAACAGA ATGTTTCAAG GAGCTGAATG TGTTCGGACC TAACGGTACC 
CTCTCACCAG ACCTGAACAG AAGTCAGCCT CCAGAACCGC CAAAGAAAGG GCTGTTCCAC 
AGACTCTTCA GGCGTCAGCA TCAAAGCAAT TCCAAGAGTT CACCTACTCC TAAGACCAGT 
TGTAACCACC GAATAAATTC AAACCACATC AATTCAAACT CCACTGGAAG CAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006526760.1
CDS	1021..2697
Translation	MELENIVANTVLLKAREDRDYYSLCDKQPIGRLLFRQFCETRPGLECYIQFLDLVAEYEITPDENLGAKGKEIMTKYLTPKSPVFIAQVGQDLVSQTEKKLLQSPCKELFSACAQSVHDYLKGDPFHEYLDSMYFDRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKRLEKKRIKKRKGESMALNEKQILEKVNSQFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNPGFEEERALFYAAEILCGLEDLHRENTVYRDLKPENILLDDYGHIRISDLGLAVKIPEGDLIRGRVGTVGYMAPEVLNNQRYGLSPDYWGLGCLIYEMIEGQSPFRGRKEKVKREEVDRRVLETEEVYSSKFSEEAKSICNMLLTKDSKQRLGCQEEGAAEVKRHPFFRNMNFKRLEAGMLDPPFVPDPRAVYCKDVLDIEQFSTVKGVNLDHTDDDFYSKFSTGSVPIPWQNEMIETECFKELNVFGPNGTLSPDLNRSQPPEPPKKGLFHRLFRRQHQSNSKSSPTPKTSCNHRINSNHINSNSTGSS

Target ORF information:

RefSeq Version	XM_006526697.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus G protein-coupled receptor kinase 5 (Grk5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006526697.4

ORF Insert Sequence:

ATGGAGCTGG AAAACATCGT GGCCAACACG GTCTTGCTGA AAGCCCGGGA AGACAGAGAT 
TACTACAGTC TATGTGACAA GCAACCAATT GGGAGACTGC TTTTTCGACA GTTCTGTGAA 
ACCAGGCCTG GGCTGGAGTG CTACATTCAG TTCCTGGACT TAGTGGCAGA ATATGAAATT 
ACTCCAGATG AAAACCTTGG GGCGAAGGGG AAGGAAATAA TGACCAAGTA CCTCACTCCA 
AAGTCCCCAG TCTTCATTGC CCAAGTTGGA CAGGACCTGG TCTCCCAGAC AGAGAAGAAG 
CTCCTGCAGA GCCCCTGCAA AGAACTCTTC TCTGCTTGTG CTCAGTCTGT CCATGACTAC 
TTGAAGGGAG ACCCCTTCCA CGAGTACCTG GATAGCATGT ATTTTGACCG TTTTCTGCAG 
TGGAAATGGT TAGAAAGACA ACCAGTGACC AAAAACACTT TCCGGCAGTA CCGAGTGCTG 
GGCAAAGGGG GCTTTGGAGA GGTCTGTGCC TGCCAGGTTC GGGCCACTGG TAAAATGTAT 
GCTTGTAAAC GCTTAGAGAA GAAGAGGATC AAAAAGAGGA AAGGCGAATC CATGGCACTC 
AACGAAAAGC AGATTCTTGA GAAGGTCAAC AGCCAGTTTG TGGTCAACCT GGCCTATGCC 
TATGAAACCA AAGATGCACT ATGCCTGGTT CTGACCATTA TGAATGGTGG TGACCTGAAG 
TTTCACATCT ACAATATGGG GAATCCTGGC TTTGAGGAAG AGCGAGCCTT ATTTTATGCA 
GCTGAGATCC TCTGTGGCCT AGAAGACTTA CACCGTGAGA ACACTGTCTA TAGAGATCTA 
AAACCCGAAA ACATCTTGCT GGATGATTAT GGCCACATAA GGATCTCAGA CCTCGGACTG 
GCCGTGAAGA TCCCCGAGGG AGACCTTATC CGTGGCCGGG TAGGCACTGT TGGCTACATG 
GCCCCAGAAG TTCTGAACAA CCAGCGATAT GGACTGAGCC CTGACTACTG GGGCCTGGGC 
TGCCTCATCT ATGAGATGAT TGAAGGCCAG TCACCATTTC GAGGTCGCAA GGAGAAGGTT 
AAGCGGGAAG AGGTGGATCG CCGGGTGCTG GAGACTGAGG AAGTGTATTC CTCCAAGTTC 
TCTGAAGAGG CCAAGTCCAT CTGCAACATG CTGCTCACCA AAGACTCGAA GCAGAGGCTG 
GGCTGCCAGG AGGAGGGGGC CGCCGAGGTC AAGAGGCACC CTTTCTTCAG GAACATGAAC 
TTTAAGCGCC TGGAGGCTGG GATGTTGGAC CCTCCCTTCG TTCCAGATCC CCGGGCTGTA 
TACTGCAAGG ATGTGCTGGA CATTGAGCAG TTCTCCACTG TGAAAGGTGT CAACCTGGAC 
CATACGGACG ATGATTTTTA CTCAAAGTTC TCTACAGGCT CTGTGCCAAT TCCATGGCAA 
AATGAGATGA TAGAAACAGA ATGTTTCAAG GAGCTGAATG TGTTCGGACC TAACGGTACC 
CTCTCACCAG ACCTGAACAG AAGTCAGCCT CCAGAACCGC CAAAGAAAGG GCTGTTCCAC 
AGACTCTTCA GGCGTCAGCA TCAAAGCAAT TCCAAGAGTT CACCTACTCC TAAGACCAGT 
TGTAACCACC GAATAAATTC AAACCACATC AATTCAAACT CCACTGGAAG CAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grk5 ( XM_011247149.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245451.1 Mus musculus G protein-coupled receptor kinase 5 (Grk5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu67848
Clone ID Related Accession (Same CDS sequence)	XM_011247149.3 , XM_011247149.2
Accession Version	XM_011247149.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	G protein-coupled receptor kinase 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011247149.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGACA GAGATTACTA CAGTCTATGT GACAAGCAAC CAATTGGGAG ACTGCTTTTT 
CGACAGTTCT GTGAAACCAG GCCTGGGCTG GAGTGCTACA TTCAGTTCCT GGACTTAGTG 
GCAGAATATG AAATTACTCC AGATGAAAAC CTTGGGGCGA AGGGGAAGGA AATAATGACC 
AAGTACCTCA CTCCAAAGTC CCCAGTCTTC ATTGCCCAAG TTGGACAGGA CCTGGTCTCC 
CAGACAGAGA AGAAGCTCCT GCAGAGCCCC TGCAAAGAAC TCTTCTCTGC TTGTGCTCAG 
TCTGTCCATG ACTACTTGAA GGGAGACCCC TTCCACGAGT ACCTGGATAG CATGTATTTT 
GACCGTTTTC TGCAGTGGAA ATGGTTAGAA AGACAACCAG TGACCAAAAA CACTTTCCGG 
CAGTACCGAG TGCTGGGCAA AGGGGGCTTT GGAGAGGTCT GTGCCTGCCA GGTTCGGGCC 
ACTGGTAAAA TGTATGCTTG TAAACGCTTA GAGAAGAAGA GGATCAAAAA GAGGAAAGGC 
GAATCCATGG CACTCAACGA AAAGCAGATT CTTGAGAAGG TCAACAGCCA GTTTGTGGTC 
AACCTGGCCT ATGCCTATGA AACCAAAGAT GCACTATGCC TGGTTCTGAC CATTATGAAT 
GGTGGTGACC TGAAGTTTCA CATCTACAAT ATGGGGAATC CTGGCTTTGA GGAAGAGCGA 
GCCTTATTTT ATGCAGCTGA GATCCTCTGT GGCCTAGAAG ACTTACACCG TGAGAACACT 
GTCTATAGAG ATCTAAAACC CGAAAACATC TTGCTGGATG ATTATGGCCA CATAAGGATC 
TCAGACCTCG GACTGGCCGT GAAGATCCCC GAGGGAGACC TTATCCGTGG CCGGGTAGGC 
ACTGTTGGCT ACATGGCCCC AGAAGTTCTG AACAACCAGC GATATGGACT GAGCCCTGAC 
TACTGGGGCC TGGGCTGCCT CATCTATGAG ATGATTGAAG GCCAGTCACC ATTTCGAGGT 
CGCAAGGAGA AGGTTAAGCG GGAAGAGGTG GATCGCCGGG TGCTGGAGAC TGAGGAAGTG 
TATTCCTCCA AGTTCTCTGA AGAGGCCAAG TCCATCTGCA ACATGCTGCT CACCAAAGAC 
TCGAAGCAGA GGCTGGGCTG CCAGGAGGAG GGGGCCGCCG AGGTCAAGAG GCACCCTTTC 
TTCAGGAACA TGAACTTTAA GCGCCTGGAG GCTGGGATGT TGGACCCTCC CTTCGTTCCA 
GATCCCCGGG CTGTATACTG CAAGGATGTG CTGGACATTG AGCAGTTCTC CACTGTGAAA 
GGTGTCAACC TGGACCATAC GGACGATGAT TTTTACTCAA AGTTCTCTAC AGGCTCTGTG 
CCAATTCCAT GGCAAAATGA GATGATAGAA ACAGAATGTT TCAAGGAGCT GAATGTGTTC 
GGACCTAACG GTACCCTCTC ACCAGACCTG AACAGAAGTC AGCCTCCAGA ACCGCCAAAG 
AAAGGGCTGT TCCACAGACT CTTCAGGCGT CAGCATCAAA GCAATTCCAA GAGTTCACCT 
ACTCCTAAGA CCAGTTGTAA CCACCGAATA AATTCAAACC ACATCAATTC AAACTCCACT 
GGAAGCAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245451.1
CDS	40..1671
Translation	MTDRDYYSLCDKQPIGRLLFRQFCETRPGLECYIQFLDLVAEYEITPDENLGAKGKEIMTKYLTPKSPVFIAQVGQDLVSQTEKKLLQSPCKELFSACAQSVHDYLKGDPFHEYLDSMYFDRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKRLEKKRIKKRKGESMALNEKQILEKVNSQFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNPGFEEERALFYAAEILCGLEDLHRENTVYRDLKPENILLDDYGHIRISDLGLAVKIPEGDLIRGRVGTVGYMAPEVLNNQRYGLSPDYWGLGCLIYEMIEGQSPFRGRKEKVKREEVDRRVLETEEVYSSKFSEEAKSICNMLLTKDSKQRLGCQEEGAAEVKRHPFFRNMNFKRLEAGMLDPPFVPDPRAVYCKDVLDIEQFSTVKGVNLDHTDDDFYSKFSTGSVPIPWQNEMIETECFKELNVFGPNGTLSPDLNRSQPPEPPKKGLFHRLFRRQHQSNSKSSPTPKTSCNHRINSNHINSNSTGSS

Target ORF information:

RefSeq Version	XM_011247149.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus G protein-coupled receptor kinase 5 (Grk5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247149.3

ORF Insert Sequence:

ATGACAGACA GAGATTACTA CAGTCTATGT GACAAGCAAC CAATTGGGAG ACTGCTTTTT 
CGACAGTTCT GTGAAACCAG GCCTGGGCTG GAGTGCTACA TTCAGTTCCT GGACTTAGTG 
GCAGAATATG AAATTACTCC AGATGAAAAC CTTGGGGCGA AGGGGAAGGA AATAATGACC 
AAGTACCTCA CTCCAAAGTC CCCAGTCTTC ATTGCCCAAG TTGGACAGGA CCTGGTCTCC 
CAGACAGAGA AGAAGCTCCT GCAGAGCCCC TGCAAAGAAC TCTTCTCTGC TTGTGCTCAG 
TCTGTCCATG ACTACTTGAA GGGAGACCCC TTCCACGAGT ACCTGGATAG CATGTATTTT 
GACCGTTTTC TGCAGTGGAA ATGGTTAGAA AGACAACCAG TGACCAAAAA CACTTTCCGG 
CAGTACCGAG TGCTGGGCAA AGGGGGCTTT GGAGAGGTCT GTGCCTGCCA GGTTCGGGCC 
ACTGGTAAAA TGTATGCTTG TAAACGCTTA GAGAAGAAGA GGATCAAAAA GAGGAAAGGC 
GAATCCATGG CACTCAACGA AAAGCAGATT CTTGAGAAGG TCAACAGCCA GTTTGTGGTC 
AACCTGGCCT ATGCCTATGA AACCAAAGAT GCACTATGCC TGGTTCTGAC CATTATGAAT 
GGTGGTGACC TGAAGTTTCA CATCTACAAT ATGGGGAATC CTGGCTTTGA GGAAGAGCGA 
GCCTTATTTT ATGCAGCTGA GATCCTCTGT GGCCTAGAAG ACTTACACCG TGAGAACACT 
GTCTATAGAG ATCTAAAACC CGAAAACATC TTGCTGGATG ATTATGGCCA CATAAGGATC 
TCAGACCTCG GACTGGCCGT GAAGATCCCC GAGGGAGACC TTATCCGTGG CCGGGTAGGC 
ACTGTTGGCT ACATGGCCCC AGAAGTTCTG AACAACCAGC GATATGGACT GAGCCCTGAC 
TACTGGGGCC TGGGCTGCCT CATCTATGAG ATGATTGAAG GCCAGTCACC ATTTCGAGGT 
CGCAAGGAGA AGGTTAAGCG GGAAGAGGTG GATCGCCGGG TGCTGGAGAC TGAGGAAGTG 
TATTCCTCCA AGTTCTCTGA AGAGGCCAAG TCCATCTGCA ACATGCTGCT CACCAAAGAC 
TCGAAGCAGA GGCTGGGCTG CCAGGAGGAG GGGGCCGCCG AGGTCAAGAG GCACCCTTTC 
TTCAGGAACA TGAACTTTAA GCGCCTGGAG GCTGGGATGT TGGACCCTCC CTTCGTTCCA 
GATCCCCGGG CTGTATACTG CAAGGATGTG CTGGACATTG AGCAGTTCTC CACTGTGAAA 
GGTGTCAACC TGGACCATAC GGACGATGAT TTTTACTCAA AGTTCTCTAC AGGCTCTGTG 
CCAATTCCAT GGCAAAATGA GATGATAGAA ACAGAATGTT TCAAGGAGCT GAATGTGTTC 
GGACCTAACG GTACCCTCTC ACCAGACCTG AACAGAAGTC AGCCTCCAGA ACCGCCAAAG 
AAAGGGCTGT TCCACAGACT CTTCAGGCGT CAGCATCAAA GCAATTCCAA GAGTTCACCT 
ACTCCTAAGA CCAGTTGTAA CCACCGAATA AATTCAAACC ACATCAATTC AAACTCCACT 
GGAAGCAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grk5 ( XM_017318066.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173555.1 Mus musculus G protein-coupled receptor kinase 5 (Grk5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu79888
Clone ID Related Accession (Same CDS sequence)	XM_017318066.1 , XM_017318066.2
Accession Version	XM_017318066.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	G protein-coupled receptor kinase 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCTG TCTCTCTTCC TATTGCTCAA GAGTCTTTCA TCCTGATTTC TGTGAAGCCA 
CTTGAAAAGC CAAGAAAATG TGATCTCTTG AGCCCGTCCC CAGTCTTCAT TGCCCAAGTT 
GGACAGGACC TGGTCTCCCA GACAGAGAAG AAGCTCCTGC AGAGCCCCTG CAAAGAACTC 
TTCTCTGCTT GTGCTCAGTC TGTCCATGAC TACTTGAAGG GAGACCCCTT CCACGAGTAC 
CTGGATAGCA TGTATTTTGA CCGTTTTCTG CAGTGGAAAT GGTTAGAAAG ACAACCAGTG 
ACCAAAAACA CTTTCCGGCA GTACCGAGTG CTGGGCAAAG GGGGCTTTGG AGAGGTCTGT 
GCCTGCCAGG TTCGGGCCAC TGGTAAAATG TATGCTTGTA AACGCTTAGA GAAGAAGAGG 
ATCAAAAAGA GGAAAGGCGA ATCCATGGCA CTCAACGAAA AGCAGATTCT TGAGAAGGTC 
AACAGCCAGT TTGTGGTCAA CCTGGCCTAT GCCTATGAAA CCAAAGATGC ACTATGCCTG 
GTTCTGACCA TTATGAATGG TGGTGACCTG AAGTTTCACA TCTACAATAT GGGGAATCCT 
GGCTTTGAGG AAGAGCGAGC CTTATTTTAT GCAGCTGAGA TCCTCTGTGG CCTAGAAGAC 
TTACACCGTG AGAACACTGT CTATAGAGAT CTAAAACCCG AAAACATCTT GCTGGATGAT 
TATGGCCACA TAAGGATCTC AGACCTCGGA CTGGCCGTGA AGATCCCCGA GGGAGACCTT 
ATCCGTGGCC GGGTAGGCAC TGTTGGCTAC ATGGCCCCAG AAGTTCTGAA CAACCAGCGA 
TATGGACTGA GCCCTGACTA CTGGGGCCTG GGCTGCCTCA TCTATGAGAT GATTGAAGGC 
CAGTCACCAT TTCGAGGTCG CAAGGAGAAG GTTAAGCGGG AAGAGGTGGA TCGCCGGGTG 
CTGGAGACTG AGGAAGTGTA TTCCTCCAAG TTCTCTGAAG AGGCCAAGTC CATCTGCAAC 
ATGCTGCTCA CCAAAGACTC GAAGCAGAGG CTGGGCTGCC AGGAGGAGGG GGCCGCCGAG 
GTCAAGAGGC ACCCTTTCTT CAGGAACATG AACTTTAAGC GCCTGGAGGC TGGGATGTTG 
GACCCTCCCT TCGTTCCAGA TCCCCGGGCT GTATACTGCA AGGATGTGCT GGACATTGAG 
CAGTTCTCCA CTGTGAAAGG TGTCAACCTG GACCATACGG ACGATGATTT TTACTCAAAG 
TTCTCTACAG GCTCTGTGCC AATTCCATGG CAAAATGAGA TGATAGAAAC AGAATGTTTC 
AAGGAGCTGA ATGTGTTCGG ACCTAACGGT ACCCTCTCAC CAGACCTGAA CAGAAGTCAG 
CCTCCAGAAC CGCCAAAGAA AGGGCTGTTC CACAGACTCT TCAGGCGTCA GCATCAAAGC 
AATTCCAAGA GTTCACCTAC TCCTAAGACC AGTTGTAACC ACCGAATAAA TTCAAACCAC 
ATCAATTCAA ACTCCACTGG AAGCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173555.1
CDS	922..2451
Translation	MFSVSLPIAQESFILISVKPLEKPRKCDLLSPSPVFIAQVGQDLVSQTEKKLLQSPCKELFSACAQSVHDYLKGDPFHEYLDSMYFDRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKRLEKKRIKKRKGESMALNEKQILEKVNSQFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNPGFEEERALFYAAEILCGLEDLHRENTVYRDLKPENILLDDYGHIRISDLGLAVKIPEGDLIRGRVGTVGYMAPEVLNNQRYGLSPDYWGLGCLIYEMIEGQSPFRGRKEKVKREEVDRRVLETEEVYSSKFSEEAKSICNMLLTKDSKQRLGCQEEGAAEVKRHPFFRNMNFKRLEAGMLDPPFVPDPRAVYCKDVLDIEQFSTVKGVNLDHTDDDFYSKFSTGSVPIPWQNEMIETECFKELNVFGPNGTLSPDLNRSQPPEPPKKGLFHRLFRRQHQSNSKSSPTPKTSCNHRINSNHINSNSTGSS

Target ORF information:

RefSeq Version	XM_017318066.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus G protein-coupled receptor kinase 5 (Grk5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318066.1

ORF Insert Sequence:

ATGTTCTCTG TCTCTCTTCC TATTGCTCAA GAGTCTTTCA TCCTGATTTC TGTGAAGCCA 
CTTGAAAAGC CAAGAAAATG TGATCTCTTG AGCCCGTCCC CAGTCTTCAT TGCCCAAGTT 
GGACAGGACC TGGTCTCCCA GACAGAGAAG AAGCTCCTGC AGAGCCCCTG CAAAGAACTC 
TTCTCTGCTT GTGCTCAGTC TGTCCATGAC TACTTGAAGG GAGACCCCTT CCACGAGTAC 
CTGGATAGCA TGTATTTTGA CCGTTTTCTG CAGTGGAAAT GGTTAGAAAG ACAACCAGTG 
ACCAAAAACA CTTTCCGGCA GTACCGAGTG CTGGGCAAAG GGGGCTTTGG AGAGGTCTGT 
GCCTGCCAGG TTCGGGCCAC TGGTAAAATG TATGCTTGTA AACGCTTAGA GAAGAAGAGG 
ATCAAAAAGA GGAAAGGCGA ATCCATGGCA CTCAACGAAA AGCAGATTCT TGAGAAGGTC 
AACAGCCAGT TTGTGGTCAA CCTGGCCTAT GCCTATGAAA CCAAAGATGC ACTATGCCTG 
GTTCTGACCA TTATGAATGG TGGTGACCTG AAGTTTCACA TCTACAATAT GGGGAATCCT 
GGCTTTGAGG AAGAGCGAGC CTTATTTTAT GCAGCTGAGA TCCTCTGTGG CCTAGAAGAC 
TTACACCGTG AGAACACTGT CTATAGAGAT CTAAAACCCG AAAACATCTT GCTGGATGAT 
TATGGCCACA TAAGGATCTC AGACCTCGGA CTGGCCGTGA AGATCCCCGA GGGAGACCTT 
ATCCGTGGCC GGGTAGGCAC TGTTGGCTAC ATGGCCCCAG AAGTTCTGAA CAACCAGCGA 
TATGGACTGA GCCCTGACTA CTGGGGCCTG GGCTGCCTCA TCTATGAGAT GATTGAAGGC 
CAGTCACCAT TTCGAGGTCG CAAGGAGAAG GTTAAGCGGG AAGAGGTGGA TCGCCGGGTG 
CTGGAGACTG AGGAAGTGTA TTCCTCCAAG TTCTCTGAAG AGGCCAAGTC CATCTGCAAC 
ATGCTGCTCA CCAAAGACTC GAAGCAGAGG CTGGGCTGCC AGGAGGAGGG GGCCGCCGAG 
GTCAAGAGGC ACCCTTTCTT CAGGAACATG AACTTTAAGC GCCTGGAGGC TGGGATGTTG 
GACCCTCCCT TCGTTCCAGA TCCCCGGGCT GTATACTGCA AGGATGTGCT GGACATTGAG 
CAGTTCTCCA CTGTGAAAGG TGTCAACCTG GACCATACGG ACGATGATTT TTACTCAAAG 
TTCTCTACAG GCTCTGTGCC AATTCCATGG CAAAATGAGA TGATAGAAAC AGAATGTTTC 
AAGGAGCTGA ATGTGTTCGG ACCTAACGGT ACCCTCTCAC CAGACCTGAA CAGAAGTCAG 
CCTCCAGAAC CGCCAAAGAA AGGGCTGTTC CACAGACTCT TCAGGCGTCA GCATCAAAGC 
AATTCCAAGA GTTCACCTAC TCCTAAGACC AGTTGTAACC ACCGAATAAA TTCAAACCAC 
ATCAATTCAA ACTCCACTGG AAGCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grk5 ( XM_017318066.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173555.1 Mus musculus G protein-coupled receptor kinase 5 (Grk5), transcript variant X3, mRNA.

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CloneID	OMu79888
Clone ID Related Accession (Same CDS sequence)	XM_017318066.1 , XM_017318066.2
Accession Version	XM_017318066.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	G protein-coupled receptor kinase 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017318066.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTCTG TCTCTCTTCC TATTGCTCAA GAGTCTTTCA TCCTGATTTC TGTGAAGCCA 
CTTGAAAAGC CAAGAAAATG TGATCTCTTG AGCCCGTCCC CAGTCTTCAT TGCCCAAGTT 
GGACAGGACC TGGTCTCCCA GACAGAGAAG AAGCTCCTGC AGAGCCCCTG CAAAGAACTC 
TTCTCTGCTT GTGCTCAGTC TGTCCATGAC TACTTGAAGG GAGACCCCTT CCACGAGTAC 
CTGGATAGCA TGTATTTTGA CCGTTTTCTG CAGTGGAAAT GGTTAGAAAG ACAACCAGTG 
ACCAAAAACA CTTTCCGGCA GTACCGAGTG CTGGGCAAAG GGGGCTTTGG AGAGGTCTGT 
GCCTGCCAGG TTCGGGCCAC TGGTAAAATG TATGCTTGTA AACGCTTAGA GAAGAAGAGG 
ATCAAAAAGA GGAAAGGCGA ATCCATGGCA CTCAACGAAA AGCAGATTCT TGAGAAGGTC 
AACAGCCAGT TTGTGGTCAA CCTGGCCTAT GCCTATGAAA CCAAAGATGC ACTATGCCTG 
GTTCTGACCA TTATGAATGG TGGTGACCTG AAGTTTCACA TCTACAATAT GGGGAATCCT 
GGCTTTGAGG AAGAGCGAGC CTTATTTTAT GCAGCTGAGA TCCTCTGTGG CCTAGAAGAC 
TTACACCGTG AGAACACTGT CTATAGAGAT CTAAAACCCG AAAACATCTT GCTGGATGAT 
TATGGCCACA TAAGGATCTC AGACCTCGGA CTGGCCGTGA AGATCCCCGA GGGAGACCTT 
ATCCGTGGCC GGGTAGGCAC TGTTGGCTAC ATGGCCCCAG AAGTTCTGAA CAACCAGCGA 
TATGGACTGA GCCCTGACTA CTGGGGCCTG GGCTGCCTCA TCTATGAGAT GATTGAAGGC 
CAGTCACCAT TTCGAGGTCG CAAGGAGAAG GTTAAGCGGG AAGAGGTGGA TCGCCGGGTG 
CTGGAGACTG AGGAAGTGTA TTCCTCCAAG TTCTCTGAAG AGGCCAAGTC CATCTGCAAC 
ATGCTGCTCA CCAAAGACTC GAAGCAGAGG CTGGGCTGCC AGGAGGAGGG GGCCGCCGAG 
GTCAAGAGGC ACCCTTTCTT CAGGAACATG AACTTTAAGC GCCTGGAGGC TGGGATGTTG 
GACCCTCCCT TCGTTCCAGA TCCCCGGGCT GTATACTGCA AGGATGTGCT GGACATTGAG 
CAGTTCTCCA CTGTGAAAGG TGTCAACCTG GACCATACGG ACGATGATTT TTACTCAAAG 
TTCTCTACAG GCTCTGTGCC AATTCCATGG CAAAATGAGA TGATAGAAAC AGAATGTTTC 
AAGGAGCTGA ATGTGTTCGG ACCTAACGGT ACCCTCTCAC CAGACCTGAA CAGAAGTCAG 
CCTCCAGAAC CGCCAAAGAA AGGGCTGTTC CACAGACTCT TCAGGCGTCA GCATCAAAGC 
AATTCCAAGA GTTCACCTAC TCCTAAGACC AGTTGTAACC ACCGAATAAA TTCAAACCAC 
ATCAATTCAA ACTCCACTGG AAGCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173555.1
CDS	954..2483
Translation	MFSVSLPIAQESFILISVKPLEKPRKCDLLSPSPVFIAQVGQDLVSQTEKKLLQSPCKELFSACAQSVHDYLKGDPFHEYLDSMYFDRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKRLEKKRIKKRKGESMALNEKQILEKVNSQFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNPGFEEERALFYAAEILCGLEDLHRENTVYRDLKPENILLDDYGHIRISDLGLAVKIPEGDLIRGRVGTVGYMAPEVLNNQRYGLSPDYWGLGCLIYEMIEGQSPFRGRKEKVKREEVDRRVLETEEVYSSKFSEEAKSICNMLLTKDSKQRLGCQEEGAAEVKRHPFFRNMNFKRLEAGMLDPPFVPDPRAVYCKDVLDIEQFSTVKGVNLDHTDDDFYSKFSTGSVPIPWQNEMIETECFKELNVFGPNGTLSPDLNRSQPPEPPKKGLFHRLFRRQHQSNSKSSPTPKTSCNHRINSNHINSNSTGSS

Target ORF information:

RefSeq Version	XM_017318066.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus G protein-coupled receptor kinase 5 (Grk5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318066.2

ORF Insert Sequence:

ATGTTCTCTG TCTCTCTTCC TATTGCTCAA GAGTCTTTCA TCCTGATTTC TGTGAAGCCA 
CTTGAAAAGC CAAGAAAATG TGATCTCTTG AGCCCGTCCC CAGTCTTCAT TGCCCAAGTT 
GGACAGGACC TGGTCTCCCA GACAGAGAAG AAGCTCCTGC AGAGCCCCTG CAAAGAACTC 
TTCTCTGCTT GTGCTCAGTC TGTCCATGAC TACTTGAAGG GAGACCCCTT CCACGAGTAC 
CTGGATAGCA TGTATTTTGA CCGTTTTCTG CAGTGGAAAT GGTTAGAAAG ACAACCAGTG 
ACCAAAAACA CTTTCCGGCA GTACCGAGTG CTGGGCAAAG GGGGCTTTGG AGAGGTCTGT 
GCCTGCCAGG TTCGGGCCAC TGGTAAAATG TATGCTTGTA AACGCTTAGA GAAGAAGAGG 
ATCAAAAAGA GGAAAGGCGA ATCCATGGCA CTCAACGAAA AGCAGATTCT TGAGAAGGTC 
AACAGCCAGT TTGTGGTCAA CCTGGCCTAT GCCTATGAAA CCAAAGATGC ACTATGCCTG 
GTTCTGACCA TTATGAATGG TGGTGACCTG AAGTTTCACA TCTACAATAT GGGGAATCCT 
GGCTTTGAGG AAGAGCGAGC CTTATTTTAT GCAGCTGAGA TCCTCTGTGG CCTAGAAGAC 
TTACACCGTG AGAACACTGT CTATAGAGAT CTAAAACCCG AAAACATCTT GCTGGATGAT 
TATGGCCACA TAAGGATCTC AGACCTCGGA CTGGCCGTGA AGATCCCCGA GGGAGACCTT 
ATCCGTGGCC GGGTAGGCAC TGTTGGCTAC ATGGCCCCAG AAGTTCTGAA CAACCAGCGA 
TATGGACTGA GCCCTGACTA CTGGGGCCTG GGCTGCCTCA TCTATGAGAT GATTGAAGGC 
CAGTCACCAT TTCGAGGTCG CAAGGAGAAG GTTAAGCGGG AAGAGGTGGA TCGCCGGGTG 
CTGGAGACTG AGGAAGTGTA TTCCTCCAAG TTCTCTGAAG AGGCCAAGTC CATCTGCAAC 
ATGCTGCTCA CCAAAGACTC GAAGCAGAGG CTGGGCTGCC AGGAGGAGGG GGCCGCCGAG 
GTCAAGAGGC ACCCTTTCTT CAGGAACATG AACTTTAAGC GCCTGGAGGC TGGGATGTTG 
GACCCTCCCT TCGTTCCAGA TCCCCGGGCT GTATACTGCA AGGATGTGCT GGACATTGAG 
CAGTTCTCCA CTGTGAAAGG TGTCAACCTG GACCATACGG ACGATGATTT TTACTCAAAG 
TTCTCTACAG GCTCTGTGCC AATTCCATGG CAAAATGAGA TGATAGAAAC AGAATGTTTC 
AAGGAGCTGA ATGTGTTCGG ACCTAACGGT ACCCTCTCAC CAGACCTGAA CAGAAGTCAG 
CCTCCAGAAC CGCCAAAGAA AGGGCTGTTC CACAGACTCT TCAGGCGTCA GCATCAAAGC 
AATTCCAAGA GTTCACCTAC TCCTAAGACC AGTTGTAACC ACCGAATAAA TTCAAACCAC 
ATCAATTCAA ACTCCACTGG AAGCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

GRK5 Regulates Social Behavior Via Suppression of mTORC1 Signaling in Medial Prefrontal Cortex.
Cerebral cortex (New York, N.Y. : 1991)28(2)421-432(2018 Feb)
Niu B,Liu P,Shen M,Liu C,Wang L,Wang F,Ma L

GRK5-Knockout Mice Generated by TALEN-Mediated Gene Targeting.
Animal biotechnology27(4)223-30(2016 Oct)
Nanjidsuren T,Park CW,Sim BW,Kim SU,Chang KT,Kang MH,Min KS

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

GRK5 Deficiency Leads to Selective Basal Forebrain Cholinergic Neuronal Vulnerability.
Scientific reports626116(2016 May)
He M,Singh P,Cheng S,Zhang Q,Peng W,Ding X,Li L,Liu J,Premont RT,Morgan D,Burns JM,Swerdlow RH,Suo WZ

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Grk5 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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