Notch4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Notch4
Entrez Gene ID	18132
Full Name	notch 4
Synonyms	Int-3,Int3,N4
General protein information	Preferred Names notch 4 Names neurogenic locus notch homolog protein 4 Notch gene homolog 4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 B1\|17 18.15 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006523798.4	XP_006523861.1	neurogenic locus notch homolog protein 4 isoform X1
XM_006523798.3	XP_006523861.1	neurogenic locus notch homolog protein 4 isoform X1
NM_010929.2	NP_035059.2	neurogenic locus notch homolog protein 4 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NOTCH4	XP_005627807.1
Homo sapiens (human)	NOTCH4	NP_004548.3
Pan troglodytes (chimpanzee)	NOTCH4	XP_518380.3
Bos taurus (cattle)	NOTCH4	NP_001193877.1
Mus musculus (house mouse)	Notch4	NP_035059.2
Rattus norvegicus (Norway rat)	Notch4	NP_001002827.1

Gene Ontology
Bibliography

Related articles in PubMed

Notch4 Negatively Regulates the Inflammatory Response to Mycobacterium tuberculosis Infection by Inhibiting TAK1 Activation.
Zheng R, Liu H, Zhou Y, Yan D, Chen J, Ma D, Feng Y, Qin L, Liu F, Huang X, Wang J, Ge B
The Journal of infectious diseases(2017 Dec)

The ANK repeats of Notch-4/Int3 activate NF-?B canonical pathway in the absence of Rbpj and causes mammary tumorigenesis.
Raafat A, Bargo S, McCurdy D, Callahan R
Scientific reports7(1)13690(2017 Oct)

Uncontrolled angiogenic precursor expansion causes coronary artery anomalies in mice lacking Pofut1.
Wang Y, Wu B, Lu P, Zhang D, Wu B, Varshney S, Del Monte-Nieto G, Zhuang Z, Charafeddine R, Kramer AH, Sibinga NE, Frangogiannis NG, Kitsis RN, Adams RH, Alitalo K, Sharp DJ, Harvey RP, Stanley P, Zhou B
Nature communications8(1)578(2017 09)

Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling.
Liu M, Liang K, Zhen J, Zhou M, Wang X, Wang Z, Wei X, Zhang Y, Sun Y, Zhou Z, Su H, Zhang C, Li N, Gao C, Peng J, Yi F
Nature communications8(1)413(2017 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sirt6 inhibits Notch1 and Notch4 transcription by deacetylating histone H3K9.
Title: Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling.

this study shows that Notch signaling regulates basophils biological function, at least partially via the modulation of MAPK
Title: Notch signaling pathway regulates the growth and the expression of inflammatory cytokines in mouse basophils.

Notch4-knock-out mice that also lacked Notch1, had defects in all vascular beds.
Title: ADAM10-Dependent Signaling Through Notch1 and Notch4 Controls Development of Organ-Specific Vascular Beds.

Report impaired Notch4 signaling in endothelial progenitor cells isolated from mouse Kawasaki disease model.
Title: Notch4 Signaling Pathway of Endothelial Progenitor Cells in a Kawasaki Disease Model Induced by Lactobacillus casei Cell Wall Extract.

Relative to air-exposed controls, ozone increased bronchoalveolar lavage fluid protein, a marker of lung permeability, in all genotypes, but significantly greater concentrations were found in Notch4-/- compared with wild-type and Notch3-/- mice.
Title: Novel Roles for Notch3 and Notch4 Receptors in Gene Expression and Susceptibility to Ozone-Induced Lung Inflammation in Mice.

The following Notch4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Notch4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu53249	XM_006523798.4 Latest version!	Mus musculus notch 4 (Notch4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu17904	NM_010929.2 Latest version!	Mus musculus notch 4 (Notch4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1448.30 $2069.00
View Old Accession Versions >>
OMu53249	XM_006523798.3	Mus musculus notch 4 (Notch4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Notch4 ( XM_006523798.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523861.1 Mus musculus notch 4 (Notch4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu53249
Clone ID Related Accession (Same CDS sequence)	XM_006523798.4 , XM_006523798.3
Accession Version	XM_006523798.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	neurogenic locus notch homolog protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523798.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCCC AGTTGCTGCT GCTGCTGCTC TTGCCACTCA ATTTCCCTGT CATCCTGACC 
AGAGTCTCTT CTCCAGAGCT TCTGTGTGGA GGATCCCCAG AGCCCTGTGC CAACGGAGGC 
ACCTGCCTGA GGCTATCTCG GGGACAAGGG ATCTGCCAGT GTGCCCCTGG ATTTCTGGGT 
GAGACTTGCC AGTTTCCTGA CCCCTGCAGG GATACCCAAC TCTGCAAGAA TGGTGGCAGC 
TGCCAAGCCC TGCTCCCCAC ACCCCCAAGC TCCCGTAGTC CTACTTCTCC ACTGACCCCT 
CACTTCTCCT GCACCTGCCC CTCTGGCTTC ACCGGTGATC GATGCCAAAC CCATCTGGAA 
GAGCTCTGTC CACCTTCTTT CTGTTCCAAC GGGGGTCACT GCTATGTTCA GGCCTCAGGC 
CGCCCACAGT GCTCCTGCGA GCCTGGGTGG ACAGGTGAGC AATGCCAGCT CCGAGACTTC 
TGCTCAGCCA ACCCCTGTGC CAACGGAGGC GTGTGCCTGG CCACATACCC CCAGATCCAG 
TGCCGCTGTC CACCTGGGTT CGAGGGTCAC ACCTGTGAAC GCGACATCAA CGAGTGCTTC 
CTGGAGCCGG GACCCTGCCC TCAGGGCACC TCCTGCCATA ACACCTTGGG TTCCTACCAG 
TGTCTCTGCC CTGTGGGGCA GGAAGGTCCC CAGTGCAAGC TCAGGAAGGG AGCCTGCCCT 
CCTGGAAGCT GTCTCAATGG GGGCACCTGC CAGCTGGTCC CAGAGGGACA CTCCACCTTT 
CATCTCTGCC TCTGTCCCCC AGGTTTCACG GGGCTGGACT GTGAGATGAA CCCAGATGAC 
TGTGTCAGGC ACCAGTGTCA GAACGGGGCC ACCTGTCTGG ATGGGCTGGA CACCTACACC 
TGCCTCTGCC CCAAGACATG GAAGGGCTGG GACTGCTCTG AAGATATAGA TGAATGTGAA 
GCCCGGGGTC CCCCTCGCTG CAGGAACGGT GGCACCTGCC AGAACACAGC TGGCAGCTTT 
CACTGTGTGT GCGTGAGTGG CTGGGGAGGC GCAGGCTGTG AGGAGAACCT GGATGACTGT 
GCAGCTGCCA CCTGTGCCCC GGGATCCACC TGCATCGACC GTGTGGGCTC CTTCTCCTGC 
CTCTGCCCAC CTGGACGCAC AGGCCTCCTG TGCCACCTGG AAGACATGTG TTTGAGTCAG 
CCGTGCCACG TGAATGCCCA GTGCAGCACC AACCCTCTGA CAGGCTCCAC CCTCTGCATA 
TGCCAGCCTG GCTACTCAGG ATCCACCTGT CACCAAGATC TGGATGAGTG CCAAATGGCC 
CAGCAAGGAC CCAGTCCCTG CGAACATGGC GGCTCCTGCA TCAACACCCC TGGCTCCTTC 
AACTGCCTCT GCCTGCCTGG TTACACGGGC TCCCGCTGTG AAGCTGACCA CAATGAGTGC 
CTGTCACAGC CCTGCCACCC AGGCAGCACC TGCCTGGACC TGCTTGCAAC CTTCCACTGC 
CTCTGCCCAC CAGGCTTGGA AGGGAGGCTC TGTGAGGTGG AGGTCAATGA GTGCACCTCT 
AATCCCTGCC TGAACCAAGC TGCCTGCCAT GACCTGCTCA ACGGCTTCCA GTGCCTCTGC 
CTTCCTGGAT TCACCGGCGC CCGATGTGAG AAAGACATGG ACGAGTGTAG CAGCACCCCC 
TGTGCCAATG GGGGGCGCTG CCGAGACCAG CCTGGAGCCT TCTACTGCGA GTGTCTCCCA 
GGCTTTGAAG GGCCACACTG TGAGAAAGAA GTGGACGAAT GTCTGAGTGA CCCCTGCCCC 
GTGGGAGCCA GCTGCCTTGA TCTCCCCGGA GCATTCTTCT GCCTCTGCCG TCCTGGTTTC 
ACAGGTCAAC TTTGTGAGGT TCCCTTGTGC ACCCCCAACA TGTGCCAACC TGGACAGCAA 
TGCCAAGGTC AGGAACACAG AGCCCCCTGC CTCTGCCCTG ACGGAAGTCC TGGCTGTGTT 
CCTGCCGAGG ACAACTGCCC CTGTCACCAT GGCCATTGCC AGAGATCCTT GTGTGTGTGT 
GATGAGGGCT GGACTGGACC AGAATGCGAG ACAGAACTGG GTGGCTGCAT CTCCACACCC 
TGTGCCCATG GGGGGACCTG CCACCCACAG CCATCTGGCT ACAACTGTAC CTGCCCTGCA 
GGCTACATGG GGTTGACCTG TAGTGAGGAG GTGACAGCTT GTCACTCAGG GCCCTGTCTC 
AATGGTGGCT CCTGCAGCAT CCGTCCTGAG GGCTATTCCT GCACCTGCCT TCCAAGTCAC 
ACAGGTCGCC ACTGCCAGAC TGCCGTGGAC CACTGTGTGT CTGCCTCGTG CCTCAATGGG 
GGTACCTGTG TGAACAAGCC TGGCACTTTC TTCTGCCTCT GTGCCACTGG CTTCCAGGGG 
CTGCACTGTG AGGAGAAGAC TAACCCCAGC TGTGCAGACA GCCCCTGCAG GAACAAGGCA 
ACCTGCCAAG ACACACCTCG AGGGGCCCGC TGCCTCTGCA GCCCTGGCTA TACAGGAAGC 
AGCTGCCAGA CTCTGATAGA CTTGTGTGCC CGGAAGCCCT GTCCACACAC TGCTCGATGC 
CTCCAGAGTG GGCCCTCGTT CCAGTGCCTG TGCCTCCAGG GATGGACAGG GGCTCTCTGT 
GACTTCCCAC TGTCCTGCCA GAAGGCCGCG ATGAGCCAAG GCATAGAGAT CTCTGGCCTG 
TGCCAGAATG GAGGCCTCTG TATTGACACG GGCTCCTCCT ATTTCTGCCG CTGCCCTCCT 
GGATTCCAAG GCAAGTTATG CCAGGATAAT GTGAACCCCT GTGAGCCCAA TCCCTGCCAT 
CACGGGTCTA CCTGTGTGCC TCAGCCCAGT GGCTATGTCT GCCAGTGTGC CCCAGGCTAT 
GAGGGACAGA ACTGCTCAAA AGTACTTGAC GCTTGTCAGT CCCAGCCCTG CCACAACCAC 
GGAACCTGTA CCTCCAGGCC TGGAGGCTTC CACTGTGCCT GCCCTCCAGG CTTCGTGGGA 
CTGCGCTGTG AGGGAGATGT GGATGAGTGT CTGGACCGGC CCTGTCACCC CTCGGGCACT 
GCAGCTTGCC ACTCTTTAGC CAACGCCTTC TACTGCCAGT GTCTGCCTGG GCACACAGGC 
CAGCGGTGTG AGGTGGAGAT GGACCTCTGT CAGAGCCAAC CCTGCTCCAA TGGAGGATCC 
TGTGAGATCA CAACAGGGCC ACCCCCTGGC TTCACCTGTC ACTGCCCCAA GGGTTTTGAA 
GGCCCCACCT GCAGCCACAA AGCCCTTTCC TGCGGCATCC ATCACTGCCA CAATGGAGGC 
CTATGTCTGC CCTCCCCTAA GCCAGGGTCA CCACCACTCT GTGCCTGCCT CAGTGGTTTT 
GGGGGCCCTG ACTGTCTGAC ACCTCCAGCT CCACCGGGCT GCGGTCCCCC CTCACCCTGC 
CTGCACAATG GTACCTGCAC TGAGACCCCT GGGTTGGGCA ACCCGGGCTT TCAATGCACC 
TGCCCTCCTG ACTCTCCAGG GCCCCGGTGT CAAAGGCCAG GGGCAAGTGG GTGTGAGGGC 
CGAGGTGGTG ATGGGACCTG CGATGCTGGC TGCAGTGGCC CAGGAGGAGA CTGGGATGGA 
GGGGACTGTT CCCTGGGGGT CCCAGACCCC TGGAAGGGCT GTCCCCCGCA TTCCCAGTGC 
TGGCTTCTGT TCCGGGACGG ACGGTGTCAC CCGCAGTGTG ACTCTGAGGA GTGTCTCTTT 
GATGGCTACG ACTGTGAAAT CCCTCTAACC TGCATCCCAG CCTATGACCA GTACTGCCGA 
GATCACTTCC ACAACGGGCA CTGTGAGAAA GGCTGCAATA ACGCTGAATG TGGCTGGGAC 
GGGGGAGACT GCAGACCAGA AGGGGAAGAC TCAGAGGGGA GGCCCTCCCT GGCCCTGCTG 
GTGGTGCTGA GGCCCCCAGC CCTGGATCAG CAGCTGCTTG CCCTGGCACG AGTGCTGTCC 
CTGACTCTGA GGGTCGGTCT CTGGGTGAGG AAGGACAGTG AAGGCAGGAA CATGGTGTTC 
CCCTATCCTG GGACCCGGGC CAAAGAGGAG CTGAGTGGAG CTAGGGATTC CTCTTCATGG 
GAAAGACAAG CCCCTCCCAC TCAGCCCCTG GGCAAGGAGA CAGAGTCTCT TGGTGCAGGG 
TTTGTGGTAG TGATGGGAGT GGATCTGTCC CGCTGTGGTC CGGAACATCC TGCGTCCCGC 
TGCCCCTGGG ACTCTGGACT CCTGCTGCGC TTCCTTGCAG CAATGGCAGC AGTGGGAGCT 
CTGGAGCCCC TGCTGCCTGG ACCCTTGCTG GCGGCTCACC CTCAAGCAGG GACCAGGCCC 
TCTGCCAACC AGCTTCCCTG GCCCATTCTA TGTTCACCAG TGGTTGGGGT GCTTCTCCTG 
GCCCTTGGGG CCCTTCTCGT CCTCCAGCTC ATTCGGCGAC GGCGACGAGA ACATGGGGCC 
CTGTGGCTGC CCCCTGGTTT CATTCGAAGG CCTCAGACAC AGCAGGCACC CCACCGGCGG 
AGGCCCCCAC TGGGCGAGGA CAACATTGGT CTTAAGGCAC TGAAGCCAGA GGCCGAAGTG 
GATGAGGATG GAGTGGCCAT GTGCTCGGGC CCTGAAGAGG GAGAGGCTGA AGAAACAGCC 
TCAGCCTCCA GGTGCCAGCT TTGGCCGCTC AACAGCGGCT GTGGAGAGCT CCCCCAGGCA 
GCCATGCTGA CCCCTCCTCA GGAGTGTGAA TCGGAGGTTC TGGATGTGGA CACCTGTGGA 
CCTGATGGGG TGACACCCCT GATGTCAGCC GTCTTCTGTG GGGGAGTGCA GTCCACGACT 
GGGGCTAGTC CACAGAGACT GGGGCTAGGA AATCTGGAAC CCTGGGAACC ACTGCTGGAT 
AGAGGGGCCT GCCCCCAGGC TCACACTGTG GGCACTGGAG AGACGCCTCT GCACCTAGCT 
GCCAGATTCT CTCGGCCAAC CGCTGCCCGC CGCCTCCTTG AGGCTGGAGC CAACCCCAAC 
CAGCCAGACC GCGCTGGGCG CACCCCACTT CACACTGCTG TGGCTGCCGA CGCTCGGGAG 
GTTTGCCAGC TCCTATTGGC CAGCAGACAG ACTACGGTGG ACGCCCGCAC AGAGGACGGG 
ACTACACCTT TGATGCTGGC TGCCAGGCTG GCCGTGGAGG ACCTGGTTGA AGAATTGATC 
GCAGCCCGAG CAGATGTAGG AGCCAGGGAT AAAAGGGGAA AAACTGCACT GCACTGGGCC 
GCTGCTGTGA ACAACGCCCG AGCCGCCCGC TCTCTCCTCC AGGCTGGAGC GGATAAAGAT 
GCCCAGGACA GTAGGGAACA GACGCCGCTT TTCCTGGCAG CGCGCGAAGG AGCCGTGGAG 
GTGGCGCAGC TGTTGCTGGA GCTCGGGGCG GCCCGGGGAC TGCGAGACCA GGCCGGGCTG 
GCCCCAGGAG ATGTGGCCCG CCAGCGCAGT CACTGGGACC TGCTAACGCT GCTGGAAGGG 
GCTGGACCGA CTACGCAGGA GGCCCGTGCG CACGCACGCA CCACGCCGGG GGGCGGGGCC 
GCGCCGCGCT GCCGGACGCT GTCTGCGGGA GCGCGCCCGC GCGGGGGCGG AGCCTGTCTG 
CAGGCTCGCA CTTGGTCGGT GGACTTGGGA GCGCGCGGAG GGAAGGTGTA TGCTCGCTGC 
CGGAGCCGAT CTGGAAGCTG CGGAGGCCCC ACCACGCGCG GCCGCAGGTT CTCCGCGGGC 
TCCCGTGGAC GACGCGGGGC TAGGGCATCA CAGGATGACT GGCCTCGCGA CTGGGTGGCC 
CTGGAAGCCT GCGGCTCCGC CTGCAGTGCG CCGATCCCGC CTCCCAGCCT GACCCCGTCC 
CCAGAACGTG GATCCCCTCA AGTTGCCTGG GGTCTTCCAG TTCACCAAGA GATTCCCTTA 
AACTCGGTTG TAAGAAATCT GAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523861.1
CDS	161..6067
Translation	MQPQLLLLLLLPLNFPVILTRVSSPELLCGGSPEPCANGGTCLRLSRGQGICQCAPGFLGETCQFPDPCRDTQLCKNGGSCQALLPTPPSSRSPTSPLTPHFSCTCPSGFTGDRCQTHLEELCPPSFCSNGGHCYVQASGRPQCSCEPGWTGEQCQLRDFCSANPCANGGVCLATYPQIQCRCPPGFEGHTCERDINECFLEPGPCPQGTSCHNTLGSYQCLCPVGQEGPQCKLRKGACPPGSCLNGGTCQLVPEGHSTFHLCLCPPGFTGLDCEMNPDDCVRHQCQNGATCLDGLDTYTCLCPKTWKGWDCSEDIDECEARGPPRCRNGGTCQNTAGSFHCVCVSGWGGAGCEENLDDCAAATCAPGSTCIDRVGSFSCLCPPGRTGLLCHLEDMCLSQPCHVNAQCSTNPLTGSTLCICQPGYSGSTCHQDLDECQMAQQGPSPCEHGGSCINTPGSFNCLCLPGYTGSRCEADHNECLSQPCHPGSTCLDLLATFHCLCPPGLEGRLCEVEVNECTSNPCLNQAACHDLLNGFQCLCLPGFTGARCEKDMDECSSTPCANGGRCRDQPGAFYCECLPGFEGPHCEKEVDECLSDPCPVGASCLDLPGAFFCLCRPGFTGQLCEVPLCTPNMCQPGQQCQGQEHRAPCLCPDGSPGCVPAEDNCPCHHGHCQRSLCVCDEGWTGPECETELGGCISTPCAHGGTCHPQPSGYNCTCPAGYMGLTCSEEVTACHSGPCLNGGSCSIRPEGYSCTCLPSHTGRHCQTAVDHCVSASCLNGGTCVNKPGTFFCLCATGFQGLHCEEKTNPSCADSPCRNKATCQDTPRGARCLCSPGYTGSSCQTLIDLCARKPCPHTARCLQSGPSFQCLCLQGWTGALCDFPLSCQKAAMSQGIEISGLCQNGGLCIDTGSSYFCRCPPGFQGKLCQDNVNPCEPNPCHHGSTCVPQPSGYVCQCAPGYEGQNCSKVLDACQSQPCHNHGTCTSRPGGFHCACPPGFVGLRCEGDVDECLDRPCHPSGTAACHSLANAFYCQCLPGHTGQRCEVEMDLCQSQPCSNGGSCEITTGPPPGFTCHCPKGFEGPTCSHKALSCGIHHCHNGGLCLPSPKPGSPPLCACLSGFGGPDCLTPPAPPGCGPPSPCLHNGTCTETPGLGNPGFQCTCPPDSPGPRCQRPGASGCEGRGGDGTCDAGCSGPGGDWDGGDCSLGVPDPWKGCPPHSQCWLLFRDGRCHPQCDSEECLFDGYDCEIPLTCIPAYDQYCRDHFHNGHCEKGCNNAECGWDGGDCRPEGEDSEGRPSLALLVVLRPPALDQQLLALARVLSLTLRVGLWVRKDSEGRNMVFPYPGTRAKEELSGARDSSSWERQAPPTQPLGKETESLGAGFVVVMGVDLSRCGPEHPASRCPWDSGLLLRFLAAMAAVGALEPLLPGPLLAAHPQAGTRPSANQLPWPILCSPVVGVLLLALGALLVLQLIRRRRREHGALWLPPGFIRRPQTQQAPHRRRPPLGEDNIGLKALKPEAEVDEDGVAMCSGPEEGEAEETASASRCQLWPLNSGCGELPQAAMLTPPQECESEVLDVDTCGPDGVTPLMSAVFCGGVQSTTGASPQRLGLGNLEPWEPLLDRGACPQAHTVGTGETPLHLAARFSRPTAARRLLEAGANPNQPDRAGRTPLHTAVAADAREVCQLLLASRQTTVDARTEDGTTPLMLAARLAVEDLVEELIAARADVGARDKRGKTALHWAAAVNNARAARSLLQAGADKDAQDSREQTPLFLAAREGAVEVAQLLLELGAARGLRDQAGLAPGDVARQRSHWDLLTLLEGAGPTTQEARAHARTTPGGGAAPRCRTLSAGARPRGGGACLQARTWSVDLGARGGKVYARCRSRSGSCGGPTTRGRRFSAGSRGRRGARASQDDWPRDWVALEACGSACSAPIPPPSLTPSPERGSPQVAWGLPVHQEIPLNSVVRNLN

Target ORF information:

RefSeq Version	XM_006523798.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus notch 4 (Notch4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523798.4

ORF Insert Sequence:

ATGCAGCCCC AGTTGCTGCT GCTGCTGCTC TTGCCACTCA ATTTCCCTGT CATCCTGACC 
AGAGTCTCTT CTCCAGAGCT TCTGTGTGGA GGATCCCCAG AGCCCTGTGC CAACGGAGGC 
ACCTGCCTGA GGCTATCTCG GGGACAAGGG ATCTGCCAGT GTGCCCCTGG ATTTCTGGGT 
GAGACTTGCC AGTTTCCTGA CCCCTGCAGG GATACCCAAC TCTGCAAGAA TGGTGGCAGC 
TGCCAAGCCC TGCTCCCCAC ACCCCCAAGC TCCCGTAGTC CTACTTCTCC ACTGACCCCT 
CACTTCTCCT GCACCTGCCC CTCTGGCTTC ACCGGTGATC GATGCCAAAC CCATCTGGAA 
GAGCTCTGTC CACCTTCTTT CTGTTCCAAC GGGGGTCACT GCTATGTTCA GGCCTCAGGC 
CGCCCACAGT GCTCCTGCGA GCCTGGGTGG ACAGGTGAGC AATGCCAGCT CCGAGACTTC 
TGCTCAGCCA ACCCCTGTGC CAACGGAGGC GTGTGCCTGG CCACATACCC CCAGATCCAG 
TGCCGCTGTC CACCTGGGTT CGAGGGTCAC ACCTGTGAAC GCGACATCAA CGAGTGCTTC 
CTGGAGCCGG GACCCTGCCC TCAGGGCACC TCCTGCCATA ACACCTTGGG TTCCTACCAG 
TGTCTCTGCC CTGTGGGGCA GGAAGGTCCC CAGTGCAAGC TCAGGAAGGG AGCCTGCCCT 
CCTGGAAGCT GTCTCAATGG GGGCACCTGC CAGCTGGTCC CAGAGGGACA CTCCACCTTT 
CATCTCTGCC TCTGTCCCCC AGGTTTCACG GGGCTGGACT GTGAGATGAA CCCAGATGAC 
TGTGTCAGGC ACCAGTGTCA GAACGGGGCC ACCTGTCTGG ATGGGCTGGA CACCTACACC 
TGCCTCTGCC CCAAGACATG GAAGGGCTGG GACTGCTCTG AAGATATAGA TGAATGTGAA 
GCCCGGGGTC CCCCTCGCTG CAGGAACGGT GGCACCTGCC AGAACACAGC TGGCAGCTTT 
CACTGTGTGT GCGTGAGTGG CTGGGGAGGC GCAGGCTGTG AGGAGAACCT GGATGACTGT 
GCAGCTGCCA CCTGTGCCCC GGGATCCACC TGCATCGACC GTGTGGGCTC CTTCTCCTGC 
CTCTGCCCAC CTGGACGCAC AGGCCTCCTG TGCCACCTGG AAGACATGTG TTTGAGTCAG 
CCGTGCCACG TGAATGCCCA GTGCAGCACC AACCCTCTGA CAGGCTCCAC CCTCTGCATA 
TGCCAGCCTG GCTACTCAGG ATCCACCTGT CACCAAGATC TGGATGAGTG CCAAATGGCC 
CAGCAAGGAC CCAGTCCCTG CGAACATGGC GGCTCCTGCA TCAACACCCC TGGCTCCTTC 
AACTGCCTCT GCCTGCCTGG TTACACGGGC TCCCGCTGTG AAGCTGACCA CAATGAGTGC 
CTGTCACAGC CCTGCCACCC AGGCAGCACC TGCCTGGACC TGCTTGCAAC CTTCCACTGC 
CTCTGCCCAC CAGGCTTGGA AGGGAGGCTC TGTGAGGTGG AGGTCAATGA GTGCACCTCT 
AATCCCTGCC TGAACCAAGC TGCCTGCCAT GACCTGCTCA ACGGCTTCCA GTGCCTCTGC 
CTTCCTGGAT TCACCGGCGC CCGATGTGAG AAAGACATGG ACGAGTGTAG CAGCACCCCC 
TGTGCCAATG GGGGGCGCTG CCGAGACCAG CCTGGAGCCT TCTACTGCGA GTGTCTCCCA 
GGCTTTGAAG GGCCACACTG TGAGAAAGAA GTGGACGAAT GTCTGAGTGA CCCCTGCCCC 
GTGGGAGCCA GCTGCCTTGA TCTCCCCGGA GCATTCTTCT GCCTCTGCCG TCCTGGTTTC 
ACAGGTCAAC TTTGTGAGGT TCCCTTGTGC ACCCCCAACA TGTGCCAACC TGGACAGCAA 
TGCCAAGGTC AGGAACACAG AGCCCCCTGC CTCTGCCCTG ACGGAAGTCC TGGCTGTGTT 
CCTGCCGAGG ACAACTGCCC CTGTCACCAT GGCCATTGCC AGAGATCCTT GTGTGTGTGT 
GATGAGGGCT GGACTGGACC AGAATGCGAG ACAGAACTGG GTGGCTGCAT CTCCACACCC 
TGTGCCCATG GGGGGACCTG CCACCCACAG CCATCTGGCT ACAACTGTAC CTGCCCTGCA 
GGCTACATGG GGTTGACCTG TAGTGAGGAG GTGACAGCTT GTCACTCAGG GCCCTGTCTC 
AATGGTGGCT CCTGCAGCAT CCGTCCTGAG GGCTATTCCT GCACCTGCCT TCCAAGTCAC 
ACAGGTCGCC ACTGCCAGAC TGCCGTGGAC CACTGTGTGT CTGCCTCGTG CCTCAATGGG 
GGTACCTGTG TGAACAAGCC TGGCACTTTC TTCTGCCTCT GTGCCACTGG CTTCCAGGGG 
CTGCACTGTG AGGAGAAGAC TAACCCCAGC TGTGCAGACA GCCCCTGCAG GAACAAGGCA 
ACCTGCCAAG ACACACCTCG AGGGGCCCGC TGCCTCTGCA GCCCTGGCTA TACAGGAAGC 
AGCTGCCAGA CTCTGATAGA CTTGTGTGCC CGGAAGCCCT GTCCACACAC TGCTCGATGC 
CTCCAGAGTG GGCCCTCGTT CCAGTGCCTG TGCCTCCAGG GATGGACAGG GGCTCTCTGT 
GACTTCCCAC TGTCCTGCCA GAAGGCCGCG ATGAGCCAAG GCATAGAGAT CTCTGGCCTG 
TGCCAGAATG GAGGCCTCTG TATTGACACG GGCTCCTCCT ATTTCTGCCG CTGCCCTCCT 
GGATTCCAAG GCAAGTTATG CCAGGATAAT GTGAACCCCT GTGAGCCCAA TCCCTGCCAT 
CACGGGTCTA CCTGTGTGCC TCAGCCCAGT GGCTATGTCT GCCAGTGTGC CCCAGGCTAT 
GAGGGACAGA ACTGCTCAAA AGTACTTGAC GCTTGTCAGT CCCAGCCCTG CCACAACCAC 
GGAACCTGTA CCTCCAGGCC TGGAGGCTTC CACTGTGCCT GCCCTCCAGG CTTCGTGGGA 
CTGCGCTGTG AGGGAGATGT GGATGAGTGT CTGGACCGGC CCTGTCACCC CTCGGGCACT 
GCAGCTTGCC ACTCTTTAGC CAACGCCTTC TACTGCCAGT GTCTGCCTGG GCACACAGGC 
CAGCGGTGTG AGGTGGAGAT GGACCTCTGT CAGAGCCAAC CCTGCTCCAA TGGAGGATCC 
TGTGAGATCA CAACAGGGCC ACCCCCTGGC TTCACCTGTC ACTGCCCCAA GGGTTTTGAA 
GGCCCCACCT GCAGCCACAA AGCCCTTTCC TGCGGCATCC ATCACTGCCA CAATGGAGGC 
CTATGTCTGC CCTCCCCTAA GCCAGGGTCA CCACCACTCT GTGCCTGCCT CAGTGGTTTT 
GGGGGCCCTG ACTGTCTGAC ACCTCCAGCT CCACCGGGCT GCGGTCCCCC CTCACCCTGC 
CTGCACAATG GTACCTGCAC TGAGACCCCT GGGTTGGGCA ACCCGGGCTT TCAATGCACC 
TGCCCTCCTG ACTCTCCAGG GCCCCGGTGT CAAAGGCCAG GGGCAAGTGG GTGTGAGGGC 
CGAGGTGGTG ATGGGACCTG CGATGCTGGC TGCAGTGGCC CAGGAGGAGA CTGGGATGGA 
GGGGACTGTT CCCTGGGGGT CCCAGACCCC TGGAAGGGCT GTCCCCCGCA TTCCCAGTGC 
TGGCTTCTGT TCCGGGACGG ACGGTGTCAC CCGCAGTGTG ACTCTGAGGA GTGTCTCTTT 
GATGGCTACG ACTGTGAAAT CCCTCTAACC TGCATCCCAG CCTATGACCA GTACTGCCGA 
GATCACTTCC ACAACGGGCA CTGTGAGAAA GGCTGCAATA ACGCTGAATG TGGCTGGGAC 
GGGGGAGACT GCAGACCAGA AGGGGAAGAC TCAGAGGGGA GGCCCTCCCT GGCCCTGCTG 
GTGGTGCTGA GGCCCCCAGC CCTGGATCAG CAGCTGCTTG CCCTGGCACG AGTGCTGTCC 
CTGACTCTGA GGGTCGGTCT CTGGGTGAGG AAGGACAGTG AAGGCAGGAA CATGGTGTTC 
CCCTATCCTG GGACCCGGGC CAAAGAGGAG CTGAGTGGAG CTAGGGATTC CTCTTCATGG 
GAAAGACAAG CCCCTCCCAC TCAGCCCCTG GGCAAGGAGA CAGAGTCTCT TGGTGCAGGG 
TTTGTGGTAG TGATGGGAGT GGATCTGTCC CGCTGTGGTC CGGAACATCC TGCGTCCCGC 
TGCCCCTGGG ACTCTGGACT CCTGCTGCGC TTCCTTGCAG CAATGGCAGC AGTGGGAGCT 
CTGGAGCCCC TGCTGCCTGG ACCCTTGCTG GCGGCTCACC CTCAAGCAGG GACCAGGCCC 
TCTGCCAACC AGCTTCCCTG GCCCATTCTA TGTTCACCAG TGGTTGGGGT GCTTCTCCTG 
GCCCTTGGGG CCCTTCTCGT CCTCCAGCTC ATTCGGCGAC GGCGACGAGA ACATGGGGCC 
CTGTGGCTGC CCCCTGGTTT CATTCGAAGG CCTCAGACAC AGCAGGCACC CCACCGGCGG 
AGGCCCCCAC TGGGCGAGGA CAACATTGGT CTTAAGGCAC TGAAGCCAGA GGCCGAAGTG 
GATGAGGATG GAGTGGCCAT GTGCTCGGGC CCTGAAGAGG GAGAGGCTGA AGAAACAGCC 
TCAGCCTCCA GGTGCCAGCT TTGGCCGCTC AACAGCGGCT GTGGAGAGCT CCCCCAGGCA 
GCCATGCTGA CCCCTCCTCA GGAGTGTGAA TCGGAGGTTC TGGATGTGGA CACCTGTGGA 
CCTGATGGGG TGACACCCCT GATGTCAGCC GTCTTCTGTG GGGGAGTGCA GTCCACGACT 
GGGGCTAGTC CACAGAGACT GGGGCTAGGA AATCTGGAAC CCTGGGAACC ACTGCTGGAT 
AGAGGGGCCT GCCCCCAGGC TCACACTGTG GGCACTGGAG AGACGCCTCT GCACCTAGCT 
GCCAGATTCT CTCGGCCAAC CGCTGCCCGC CGCCTCCTTG AGGCTGGAGC CAACCCCAAC 
CAGCCAGACC GCGCTGGGCG CACCCCACTT CACACTGCTG TGGCTGCCGA CGCTCGGGAG 
GTTTGCCAGC TCCTATTGGC CAGCAGACAG ACTACGGTGG ACGCCCGCAC AGAGGACGGG 
ACTACACCTT TGATGCTGGC TGCCAGGCTG GCCGTGGAGG ACCTGGTTGA AGAATTGATC 
GCAGCCCGAG CAGATGTAGG AGCCAGGGAT AAAAGGGGAA AAACTGCACT GCACTGGGCC 
GCTGCTGTGA ACAACGCCCG AGCCGCCCGC TCTCTCCTCC AGGCTGGAGC GGATAAAGAT 
GCCCAGGACA GTAGGGAACA GACGCCGCTT TTCCTGGCAG CGCGCGAAGG AGCCGTGGAG 
GTGGCGCAGC TGTTGCTGGA GCTCGGGGCG GCCCGGGGAC TGCGAGACCA GGCCGGGCTG 
GCCCCAGGAG ATGTGGCCCG CCAGCGCAGT CACTGGGACC TGCTAACGCT GCTGGAAGGG 
GCTGGACCGA CTACGCAGGA GGCCCGTGCG CACGCACGCA CCACGCCGGG GGGCGGGGCC 
GCGCCGCGCT GCCGGACGCT GTCTGCGGGA GCGCGCCCGC GCGGGGGCGG AGCCTGTCTG 
CAGGCTCGCA CTTGGTCGGT GGACTTGGGA GCGCGCGGAG GGAAGGTGTA TGCTCGCTGC 
CGGAGCCGAT CTGGAAGCTG CGGAGGCCCC ACCACGCGCG GCCGCAGGTT CTCCGCGGGC 
TCCCGTGGAC GACGCGGGGC TAGGGCATCA CAGGATGACT GGCCTCGCGA CTGGGTGGCC 
CTGGAAGCCT GCGGCTCCGC CTGCAGTGCG CCGATCCCGC CTCCCAGCCT GACCCCGTCC 
CCAGAACGTG GATCCCCTCA AGTTGCCTGG GGTCTTCCAG TTCACCAAGA GATTCCCTTA 
AACTCGGTTG TAAGAAATCT GAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Notch4 ( NM_010929.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035059.2 Mus musculus notch 4 (Notch4), mRNA.

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CloneID	OMu17904
Clone ID Related Accession (Same CDS sequence)	NM_010929.2
Accession Version	NM_010929.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5895bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	neurogenic locus notch homolog protein 4 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT009767.6. On Sep 14, 2006 this sequence version replaced NM_010929.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M80456.1, BC138044.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCAGCCCC AGTTGCTGCT GCTGCTGCTC TTGCCACTCA ATTTCCCTGT CATCCTGACC 
AGAGAGCTTC TGTGTGGAGG ATCCCCAGAG CCCTGTGCCA ACGGAGGCAC CTGCCTGAGG 
CTATCTCGGG GACAAGGGAT CTGCCAGTGT GCCCCTGGAT TTCTGGGTGA GACTTGCCAG 
TTTCCTGACC CCTGCAGGGA TACCCAACTC TGCAAGAATG GTGGCAGCTG CCAAGCCCTG 
CTCCCCACAC CCCCAAGCTC CCGTAGTCCT ACTTCTCCAC TGACCCCTCA CTTCTCCTGC 
ACCTGCCCCT CTGGCTTCAC CGGTGATCGA TGCCAAACCC ATCTGGAAGA GCTCTGTCCA 
CCTTCTTTCT GTTCCAACGG GGGTCACTGC TATGTTCAGG CCTCAGGCCG CCCACAGTGC 
TCCTGCGAGC CTGGGTGGAC AGGTGAGCAA TGCCAGCTCC GAGACTTCTG CTCAGCCAAC 
CCCTGTGCCA ACGGAGGCGT GTGCCTGGCC ACATACCCCC AGATCCAGTG CCGCTGTCCA 
CCTGGGTTCG AGGGTCACAC CTGTGAACGC GACATCAACG AGTGCTTCCT GGAGCCGGGA 
CCCTGCCCTC AGGGCACCTC CTGCCATAAC ACCTTGGGTT CCTACCAGTG TCTCTGCCCT 
GTGGGGCAGG AAGGTCCCCA GTGCAAGCTC AGGAAGGGAG CCTGCCCTCC TGGAAGCTGT 
CTCAATGGGG GCACCTGCCA GCTGGTCCCA GAGGGACACT CCACCTTTCA TCTCTGCCTC 
TGTCCCCCAG GTTTCACGGG GCTGGACTGT GAGATGAACC CAGATGACTG TGTCAGGCAC 
CAGTGTCAGA ACGGGGCCAC CTGTCTGGAT GGGCTGGACA CCTACACCTG CCTCTGCCCC 
AAGACATGGA AGGGCTGGGA CTGCTCTGAA GATATAGATG AATGTGAAGC CCGGGGTCCC 
CCTCGCTGCA GGAACGGTGG CACCTGCCAG AACACAGCTG GCAGCTTTCA CTGTGTGTGC 
GTGAGTGGCT GGGGAGGCGC AGGCTGTGAG GAGAACCTGG ATGACTGTGC AGCTGCCACC 
TGTGCCCCGG GATCCACCTG CATCGACCGT GTGGGCTCCT TCTCCTGCCT CTGCCCACCT 
GGACGCACAG GCCTCCTGTG CCACCTGGAA GACATGTGTT TGAGTCAGCC GTGCCACGTG 
AATGCCCAGT GCAGCACCAA CCCTCTGACA GGCTCCACCC TCTGCATATG CCAGCCTGGC 
TACTCAGGAT CCACCTGTCA CCAAGATCTG GATGAGTGCC AAATGGCCCA GCAAGGACCC 
AGTCCCTGCG AACATGGCGG CTCCTGCATC AACACCCCTG GCTCCTTCAA CTGCCTCTGC 
CTGCCTGGTT ACACGGGCTC CCGCTGTGAA GCTGACCACA ATGAGTGCCT GTCACAGCCC 
TGCCACCCAG GCAGCACCTG CCTGGACCTG CTTGCAACCT TCCACTGCCT CTGCCCACCA 
GGCTTGGAAG GGAGGCTCTG TGAGGTGGAG GTCAATGAGT GCACCTCTAA TCCCTGCCTG 
AACCAAGCTG CCTGCCATGA CCTGCTCAAC GGCTTCCAGT GCCTCTGCCT TCCTGGATTC 
ACCGGCGCCC GATGTGAGAA AGACATGGAC GAGTGTAGCA GCACCCCCTG TGCCAATGGG 
GGGCGCTGCC GAGACCAGCC TGGAGCCTTC TACTGCGAGT GTCTCCCAGG CTTTGAAGGG 
CCACACTGTG AGAAAGAAGT GGACGAATGT CTGAGTGACC CCTGCCCCGT GGGAGCCAGC 
TGCCTTGATC TCCCCGGAGC ATTCTTCTGC CTCTGCCGTC CTGGTTTCAC AGGTCAACTT 
TGTGAGGTTC CCTTGTGCAC CCCCAACATG TGCCAACCTG GACAGCAATG CCAAGGTCAG 
GAACACAGAG CCCCCTGCCT CTGCCCTGAC GGAAGTCCTG GCTGTGTTCC TGCCGAGGAC 
AACTGCCCCT GTCACCATGG CCATTGCCAG AGATCCTTGT GTGTGTGTGA TGAGGGCTGG 
ACTGGACCAG AATGCGAGAC AGAACTGGGT GGCTGCATCT CCACACCCTG TGCCCATGGG 
GGGACCTGCC ACCCACAGCC ATCTGGCTAC AACTGTACCT GCCCTGCAGG CTACATGGGG 
TTGACCTGTA GTGAGGAGGT GACAGCTTGT CACTCAGGGC CCTGTCTCAA TGGTGGCTCC 
TGCAGCATCC GTCCTGAGGG CTATTCCTGC ACCTGCCTTC CAAGTCACAC AGGTCGCCAC 
TGCCAGACTG CCGTGGACCA CTGTGTGTCT GCCTCGTGCC TCAATGGGGG TACCTGTGTG 
AACAAGCCTG GCACTTTCTT CTGCCTCTGT GCCACTGGCT TCCAGGGGCT GCACTGTGAG 
GAGAAGACTA ACCCCAGCTG TGCAGACAGC CCCTGCAGGA ACAAGGCAAC CTGCCAAGAC 
ACACCTCGAG GGGCCCGCTG CCTCTGCAGC CCTGGCTATA CAGGAAGCAG CTGCCAGACT 
CTGATAGACT TGTGTGCCCG GAAGCCCTGT CCACACACTG CTCGATGCCT CCAGAGTGGG 
CCCTCGTTCC AGTGCCTGTG CCTCCAGGGA TGGACAGGGG CTCTCTGTGA CTTCCCACTG 
TCCTGCCAGA AGGCCGCGAT GAGCCAAGGC ATAGAGATCT CTGGCCTGTG CCAGAATGGA 
GGCCTCTGTA TTGACACGGG CTCCTCCTAT TTCTGCCGCT GCCCTCCTGG ATTCCAAGGC 
AAGTTATGCC AGGATAATGT GAACCCCTGT GAGCCCAATC CCTGCCATCA CGGGTCTACC 
TGTGTGCCTC AGCCCAGTGG CTATGTCTGC CAGTGTGCCC CAGGCTATGA GGGACAGAAC 
TGCTCAAAAG TACTTGACGC TTGTCAGTCC CAGCCCTGCC ACAACCACGG AACCTGTACC 
TCCAGGCCTG GAGGCTTCCA CTGTGCCTGC CCTCCAGGCT TCGTGGGACT GCGCTGTGAG 
GGAGATGTGG ATGAGTGTCT GGACCGGCCC TGTCACCCCT CGGGCACTGC AGCTTGCCAC 
TCTTTAGCCA ACGCCTTCTA CTGCCAGTGT CTGCCTGGGC ACACAGGCCA GCGGTGTGAG 
GTGGAGATGG ACCTCTGTCA GAGCCAACCC TGCTCCAATG GAGGATCCTG TGAGATCACA 
ACAGGGCCAC CCCCTGGCTT CACCTGTCAC TGCCCCAAGG GTTTTGAAGG CCCCACCTGC 
AGCCACAAAG CCCTTTCCTG CGGCATCCAT CACTGCCACA ATGGAGGCCT ATGTCTGCCC 
TCCCCTAAGC CAGGGTCACC ACCACTCTGT GCCTGCCTCA GTGGTTTTGG GGGCCCTGAC 
TGTCTGACAC CTCCAGCTCC ACCGGGCTGC GGTCCCCCCT CACCCTGCCT GCACAATGGT 
ACCTGCACTG AGACCCCTGG GTTGGGCAAC CCGGGCTTTC AATGCACCTG CCCTCCTGAC 
TCTCCAGGGC CCCGGTGTCA AAGGCCAGGG GCAAGTGGGT GTGAGGGCCG AGGTGGTGAT 
GGGACCTGCG ATGCTGGCTG CAGTGGCCCA GGAGGAGACT GGGATGGAGG GGACTGTTCC 
CTGGGGGTCC CAGACCCCTG GAAGGGCTGT CCCCCGCATT CCCAGTGCTG GCTTCTGTTC 
CGGGACGGAC GGTGTCACCC GCAGTGTGAC TCTGAGGAGT GTCTCTTTGA TGGCTACGAC 
TGTGAAATCC CTCTAACCTG CATCCCAGCC TATGACCAGT ACTGCCGAGA TCACTTCCAC 
AACGGGCACT GTGAGAAAGG CTGCAATAAC GCTGAATGTG GCTGGGACGG GGGAGACTGC 
AGACCAGAAG GGGAAGACTC AGAGGGGAGG CCCTCCCTGG CCCTGCTGGT GGTGCTGAGG 
CCCCCAGCCC TGGATCAGCA GCTGCTTGCC CTGGCACGAG TGCTGTCCCT GACTCTGAGG 
GTCGGTCTCT GGGTGAGGAA GGACAGTGAA GGCAGGAACA TGGTGTTCCC CTATCCTGGG 
ACCCGGGCCA AAGAGGAGCT GAGTGGAGCT AGGGATTCCT CTTCATGGGA AAGACAAGCC 
CCTCCCACTC AGCCCCTGGG CAAGGAGACA GAGTCTCTTG GTGCAGGGTT TGTGGTAGTG 
ATGGGAGTGG ATCTGTCCCG CTGTGGTCCG GAACATCCTG CGTCCCGCTG CCCCTGGGAC 
TCTGGACTCC TGCTGCGCTT CCTTGCAGCA ATGGCAGCAG TGGGAGCTCT GGAGCCCCTG 
CTGCCTGGAC CCTTGCTGGC GGCTCACCCT CAAGCAGGGA CCAGGCCCTC TGCCAACCAG 
CTTCCCTGGC CCATTCTATG TTCACCAGTG GTTGGGGTGC TTCTCCTGGC CCTTGGGGCC 
CTTCTCGTCC TCCAGCTCAT TCGGCGACGG CGACGAGAAC ATGGGGCCCT GTGGCTGCCC 
CCTGGTTTCA TTCGAAGGCC TCAGACACAG CAGGCACCCC ACCGGCGGAG GCCCCCACTG 
GGCGAGGACA ACATTGGTCT TAAGGCACTG AAGCCAGAGG CCGAAGTGGA TGAGGATGGA 
GTGGCCATGT GCTCGGGCCC TGAAGAGGGA GAGGCTGAAG AAACAGCCTC AGCCTCCAGG 
TGCCAGCTTT GGCCGCTCAA CAGCGGCTGT GGAGAGCTCC CCCAGGCAGC CATGCTGACC 
CCTCCTCAGG AGTGTGAATC GGAGGTTCTG GATGTGGACA CCTGTGGACC TGATGGGGTG 
ACACCCCTGA TGTCAGCCGT CTTCTGTGGG GGAGTGCAGT CCACGACTGG GGCTAGTCCA 
CAGAGACTGG GGCTAGGAAA TCTGGAACCC TGGGAACCAC TGCTGGATAG AGGGGCCTGC 
CCCCAGGCTC ACACTGTGGG CACTGGAGAG ACGCCTCTGC ACCTAGCTGC CAGATTCTCT 
CGGCCAACCG CTGCCCGCCG CCTCCTTGAG GCTGGAGCCA ACCCCAACCA GCCAGACCGC 
GCTGGGCGCA CCCCACTTCA CACTGCTGTG GCTGCCGACG CTCGGGAGGT TTGCCAGCTC 
CTATTGGCCA GCAGACAGAC TACGGTGGAC GCCCGCACAG AGGACGGGAC TACACCTTTG 
ATGCTGGCTG CCAGGCTGGC CGTGGAGGAC CTGGTTGAAG AATTGATCGC AGCCCGAGCA 
GATGTAGGAG CCAGGGATAA AAGGGGAAAA ACTGCACTGC ACTGGGCCGC TGCTGTGAAC 
AACGCCCGAG CCGCCCGCTC TCTCCTCCAG GCTGGAGCGG ATAAAGATGC CCAGGACAGT 
AGGGAACAGA CGCCGCTTTT CCTGGCAGCG CGCGAAGGAG CCGTGGAGGT GGCGCAGCTG 
TTGCTGGAGC TCGGGGCGGC CCGGGGACTG CGAGACCAGG CCGGGCTGGC CCCAGGAGAT 
GTGGCCCGCC AGCGCAGTCA CTGGGACCTG CTAACGCTGC TGGAAGGGGC TGGACCGACT 
ACGCAGGAGG CCCGTGCGCA CGCACGCACC ACGCCGGGGG GCGGGGCCGC GCCGCGCTGC 
CGGACGCTGT CTGCGGGAGC GCGCCCGCGC GGGGGCGGAG CCTGTCTGCA GGCTCGCACT 
TGGTCGGTGG ACTTGGGAGC GCGCGGAGGG AAGGTGTATG CTCGCTGCCG GAGCCGATCT 
GGAAGCTGCG GAGGCCCCAC CACGCGCGGC CGCAGGTTCT CCGCGGGCTC CCGTGGACGA 
CGCGGGGCTA GGGCATCACA GGATGACTGG CCTCGCGACT GGGTGGCCCT GGAAGCCTGC 
GGCTCCGCCT GCAGTGCGCC GATCCCGCCT CCCAGCCTGA CCCCGTCCCC AGAACGTGGA 
TCCCCTCAAG TTGCCTGGGG TCTTCCAGTT CACCAAGAGA TTCCCTTAAA CTCGGTTGTA 
AGAAATCTGA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035059.2
CDS	152..6046
Translation	MQPQLLLLLLLPLNFPVILTRELLCGGSPEPCANGGTCLRLSRGQGICQCAPGFLGETCQFPDPCRDTQLCKNGGSCQALLPTPPSSRSPTSPLTPHFSCTCPSGFTGDRCQTHLEELCPPSFCSNGGHCYVQASGRPQCSCEPGWTGEQCQLRDFCSANPCANGGVCLATYPQIQCRCPPGFEGHTCERDINECFLEPGPCPQGTSCHNTLGSYQCLCPVGQEGPQCKLRKGACPPGSCLNGGTCQLVPEGHSTFHLCLCPPGFTGLDCEMNPDDCVRHQCQNGATCLDGLDTYTCLCPKTWKGWDCSEDIDECEARGPPRCRNGGTCQNTAGSFHCVCVSGWGGAGCEENLDDCAAATCAPGSTCIDRVGSFSCLCPPGRTGLLCHLEDMCLSQPCHVNAQCSTNPLTGSTLCICQPGYSGSTCHQDLDECQMAQQGPSPCEHGGSCINTPGSFNCLCLPGYTGSRCEADHNECLSQPCHPGSTCLDLLATFHCLCPPGLEGRLCEVEVNECTSNPCLNQAACHDLLNGFQCLCLPGFTGARCEKDMDECSSTPCANGGRCRDQPGAFYCECLPGFEGPHCEKEVDECLSDPCPVGASCLDLPGAFFCLCRPGFTGQLCEVPLCTPNMCQPGQQCQGQEHRAPCLCPDGSPGCVPAEDNCPCHHGHCQRSLCVCDEGWTGPECETELGGCISTPCAHGGTCHPQPSGYNCTCPAGYMGLTCSEEVTACHSGPCLNGGSCSIRPEGYSCTCLPSHTGRHCQTAVDHCVSASCLNGGTCVNKPGTFFCLCATGFQGLHCEEKTNPSCADSPCRNKATCQDTPRGARCLCSPGYTGSSCQTLIDLCARKPCPHTARCLQSGPSFQCLCLQGWTGALCDFPLSCQKAAMSQGIEISGLCQNGGLCIDTGSSYFCRCPPGFQGKLCQDNVNPCEPNPCHHGSTCVPQPSGYVCQCAPGYEGQNCSKVLDACQSQPCHNHGTCTSRPGGFHCACPPGFVGLRCEGDVDECLDRPCHPSGTAACHSLANAFYCQCLPGHTGQRCEVEMDLCQSQPCSNGGSCEITTGPPPGFTCHCPKGFEGPTCSHKALSCGIHHCHNGGLCLPSPKPGSPPLCACLSGFGGPDCLTPPAPPGCGPPSPCLHNGTCTETPGLGNPGFQCTCPPDSPGPRCQRPGASGCEGRGGDGTCDAGCSGPGGDWDGGDCSLGVPDPWKGCPPHSQCWLLFRDGRCHPQCDSEECLFDGYDCEIPLTCIPAYDQYCRDHFHNGHCEKGCNNAECGWDGGDCRPEGEDSEGRPSLALLVVLRPPALDQQLLALARVLSLTLRVGLWVRKDSEGRNMVFPYPGTRAKEELSGARDSSSWERQAPPTQPLGKETESLGAGFVVVMGVDLSRCGPEHPASRCPWDSGLLLRFLAAMAAVGALEPLLPGPLLAAHPQAGTRPSANQLPWPILCSPVVGVLLLALGALLVLQLIRRRRREHGALWLPPGFIRRPQTQQAPHRRRPPLGEDNIGLKALKPEAEVDEDGVAMCSGPEEGEAEETASASRCQLWPLNSGCGELPQAAMLTPPQECESEVLDVDTCGPDGVTPLMSAVFCGGVQSTTGASPQRLGLGNLEPWEPLLDRGACPQAHTVGTGETPLHLAARFSRPTAARRLLEAGANPNQPDRAGRTPLHTAVAADAREVCQLLLASRQTTVDARTEDGTTPLMLAARLAVEDLVEELIAARADVGARDKRGKTALHWAAAVNNARAARSLLQAGADKDAQDSREQTPLFLAAREGAVEVAQLLLELGAARGLRDQAGLAPGDVARQRSHWDLLTLLEGAGPTTQEARAHARTTPGGGAAPRCRTLSAGARPRGGGACLQARTWSVDLGARGGKVYARCRSRSGSCGGPTTRGRRFSAGSRGRRGARASQDDWPRDWVALEACGSACSAPIPPPSLTPSPERGSPQVAWGLPVHQEIPLNSVVRNLN

Target ORF information:

RefSeq Version	NM_010929.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus notch 4 (Notch4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010929.2

ORF Insert Sequence:

ATGCAGCCCC AGTTGCTGCT GCTGCTGCTC TTGCCACTCA ATTTCCCTGT CATCCTGACC 
AGAGAGCTTC TGTGTGGAGG ATCCCCAGAG CCCTGTGCCA ACGGAGGCAC CTGCCTGAGG 
CTATCTCGGG GACAAGGGAT CTGCCAGTGT GCCCCTGGAT TTCTGGGTGA GACTTGCCAG 
TTTCCTGACC CCTGCAGGGA TACCCAACTC TGCAAGAATG GTGGCAGCTG CCAAGCCCTG 
CTCCCCACAC CCCCAAGCTC CCGTAGTCCT ACTTCTCCAC TGACCCCTCA CTTCTCCTGC 
ACCTGCCCCT CTGGCTTCAC CGGTGATCGA TGCCAAACCC ATCTGGAAGA GCTCTGTCCA 
CCTTCTTTCT GTTCCAACGG GGGTCACTGC TATGTTCAGG CCTCAGGCCG CCCACAGTGC 
TCCTGCGAGC CTGGGTGGAC AGGTGAGCAA TGCCAGCTCC GAGACTTCTG CTCAGCCAAC 
CCCTGTGCCA ACGGAGGCGT GTGCCTGGCC ACATACCCCC AGATCCAGTG CCGCTGTCCA 
CCTGGGTTCG AGGGTCACAC CTGTGAACGC GACATCAACG AGTGCTTCCT GGAGCCGGGA 
CCCTGCCCTC AGGGCACCTC CTGCCATAAC ACCTTGGGTT CCTACCAGTG TCTCTGCCCT 
GTGGGGCAGG AAGGTCCCCA GTGCAAGCTC AGGAAGGGAG CCTGCCCTCC TGGAAGCTGT 
CTCAATGGGG GCACCTGCCA GCTGGTCCCA GAGGGACACT CCACCTTTCA TCTCTGCCTC 
TGTCCCCCAG GTTTCACGGG GCTGGACTGT GAGATGAACC CAGATGACTG TGTCAGGCAC 
CAGTGTCAGA ACGGGGCCAC CTGTCTGGAT GGGCTGGACA CCTACACCTG CCTCTGCCCC 
AAGACATGGA AGGGCTGGGA CTGCTCTGAA GATATAGATG AATGTGAAGC CCGGGGTCCC 
CCTCGCTGCA GGAACGGTGG CACCTGCCAG AACACAGCTG GCAGCTTTCA CTGTGTGTGC 
GTGAGTGGCT GGGGAGGCGC AGGCTGTGAG GAGAACCTGG ATGACTGTGC AGCTGCCACC 
TGTGCCCCGG GATCCACCTG CATCGACCGT GTGGGCTCCT TCTCCTGCCT CTGCCCACCT 
GGACGCACAG GCCTCCTGTG CCACCTGGAA GACATGTGTT TGAGTCAGCC GTGCCACGTG 
AATGCCCAGT GCAGCACCAA CCCTCTGACA GGCTCCACCC TCTGCATATG CCAGCCTGGC 
TACTCAGGAT CCACCTGTCA CCAAGATCTG GATGAGTGCC AAATGGCCCA GCAAGGACCC 
AGTCCCTGCG AACATGGCGG CTCCTGCATC AACACCCCTG GCTCCTTCAA CTGCCTCTGC 
CTGCCTGGTT ACACGGGCTC CCGCTGTGAA GCTGACCACA ATGAGTGCCT GTCACAGCCC 
TGCCACCCAG GCAGCACCTG CCTGGACCTG CTTGCAACCT TCCACTGCCT CTGCCCACCA 
GGCTTGGAAG GGAGGCTCTG TGAGGTGGAG GTCAATGAGT GCACCTCTAA TCCCTGCCTG 
AACCAAGCTG CCTGCCATGA CCTGCTCAAC GGCTTCCAGT GCCTCTGCCT TCCTGGATTC 
ACCGGCGCCC GATGTGAGAA AGACATGGAC GAGTGTAGCA GCACCCCCTG TGCCAATGGG 
GGGCGCTGCC GAGACCAGCC TGGAGCCTTC TACTGCGAGT GTCTCCCAGG CTTTGAAGGG 
CCACACTGTG AGAAAGAAGT GGACGAATGT CTGAGTGACC CCTGCCCCGT GGGAGCCAGC 
TGCCTTGATC TCCCCGGAGC ATTCTTCTGC CTCTGCCGTC CTGGTTTCAC AGGTCAACTT 
TGTGAGGTTC CCTTGTGCAC CCCCAACATG TGCCAACCTG GACAGCAATG CCAAGGTCAG 
GAACACAGAG CCCCCTGCCT CTGCCCTGAC GGAAGTCCTG GCTGTGTTCC TGCCGAGGAC 
AACTGCCCCT GTCACCATGG CCATTGCCAG AGATCCTTGT GTGTGTGTGA TGAGGGCTGG 
ACTGGACCAG AATGCGAGAC AGAACTGGGT GGCTGCATCT CCACACCCTG TGCCCATGGG 
GGGACCTGCC ACCCACAGCC ATCTGGCTAC AACTGTACCT GCCCTGCAGG CTACATGGGG 
TTGACCTGTA GTGAGGAGGT GACAGCTTGT CACTCAGGGC CCTGTCTCAA TGGTGGCTCC 
TGCAGCATCC GTCCTGAGGG CTATTCCTGC ACCTGCCTTC CAAGTCACAC AGGTCGCCAC 
TGCCAGACTG CCGTGGACCA CTGTGTGTCT GCCTCGTGCC TCAATGGGGG TACCTGTGTG 
AACAAGCCTG GCACTTTCTT CTGCCTCTGT GCCACTGGCT TCCAGGGGCT GCACTGTGAG 
GAGAAGACTA ACCCCAGCTG TGCAGACAGC CCCTGCAGGA ACAAGGCAAC CTGCCAAGAC 
ACACCTCGAG GGGCCCGCTG CCTCTGCAGC CCTGGCTATA CAGGAAGCAG CTGCCAGACT 
CTGATAGACT TGTGTGCCCG GAAGCCCTGT CCACACACTG CTCGATGCCT CCAGAGTGGG 
CCCTCGTTCC AGTGCCTGTG CCTCCAGGGA TGGACAGGGG CTCTCTGTGA CTTCCCACTG 
TCCTGCCAGA AGGCCGCGAT GAGCCAAGGC ATAGAGATCT CTGGCCTGTG CCAGAATGGA 
GGCCTCTGTA TTGACACGGG CTCCTCCTAT TTCTGCCGCT GCCCTCCTGG ATTCCAAGGC 
AAGTTATGCC AGGATAATGT GAACCCCTGT GAGCCCAATC CCTGCCATCA CGGGTCTACC 
TGTGTGCCTC AGCCCAGTGG CTATGTCTGC CAGTGTGCCC CAGGCTATGA GGGACAGAAC 
TGCTCAAAAG TACTTGACGC TTGTCAGTCC CAGCCCTGCC ACAACCACGG AACCTGTACC 
TCCAGGCCTG GAGGCTTCCA CTGTGCCTGC CCTCCAGGCT TCGTGGGACT GCGCTGTGAG 
GGAGATGTGG ATGAGTGTCT GGACCGGCCC TGTCACCCCT CGGGCACTGC AGCTTGCCAC 
TCTTTAGCCA ACGCCTTCTA CTGCCAGTGT CTGCCTGGGC ACACAGGCCA GCGGTGTGAG 
GTGGAGATGG ACCTCTGTCA GAGCCAACCC TGCTCCAATG GAGGATCCTG TGAGATCACA 
ACAGGGCCAC CCCCTGGCTT CACCTGTCAC TGCCCCAAGG GTTTTGAAGG CCCCACCTGC 
AGCCACAAAG CCCTTTCCTG CGGCATCCAT CACTGCCACA ATGGAGGCCT ATGTCTGCCC 
TCCCCTAAGC CAGGGTCACC ACCACTCTGT GCCTGCCTCA GTGGTTTTGG GGGCCCTGAC 
TGTCTGACAC CTCCAGCTCC ACCGGGCTGC GGTCCCCCCT CACCCTGCCT GCACAATGGT 
ACCTGCACTG AGACCCCTGG GTTGGGCAAC CCGGGCTTTC AATGCACCTG CCCTCCTGAC 
TCTCCAGGGC CCCGGTGTCA AAGGCCAGGG GCAAGTGGGT GTGAGGGCCG AGGTGGTGAT 
GGGACCTGCG ATGCTGGCTG CAGTGGCCCA GGAGGAGACT GGGATGGAGG GGACTGTTCC 
CTGGGGGTCC CAGACCCCTG GAAGGGCTGT CCCCCGCATT CCCAGTGCTG GCTTCTGTTC 
CGGGACGGAC GGTGTCACCC GCAGTGTGAC TCTGAGGAGT GTCTCTTTGA TGGCTACGAC 
TGTGAAATCC CTCTAACCTG CATCCCAGCC TATGACCAGT ACTGCCGAGA TCACTTCCAC 
AACGGGCACT GTGAGAAAGG CTGCAATAAC GCTGAATGTG GCTGGGACGG GGGAGACTGC 
AGACCAGAAG GGGAAGACTC AGAGGGGAGG CCCTCCCTGG CCCTGCTGGT GGTGCTGAGG 
CCCCCAGCCC TGGATCAGCA GCTGCTTGCC CTGGCACGAG TGCTGTCCCT GACTCTGAGG 
GTCGGTCTCT GGGTGAGGAA GGACAGTGAA GGCAGGAACA TGGTGTTCCC CTATCCTGGG 
ACCCGGGCCA AAGAGGAGCT GAGTGGAGCT AGGGATTCCT CTTCATGGGA AAGACAAGCC 
CCTCCCACTC AGCCCCTGGG CAAGGAGACA GAGTCTCTTG GTGCAGGGTT TGTGGTAGTG 
ATGGGAGTGG ATCTGTCCCG CTGTGGTCCG GAACATCCTG CGTCCCGCTG CCCCTGGGAC 
TCTGGACTCC TGCTGCGCTT CCTTGCAGCA ATGGCAGCAG TGGGAGCTCT GGAGCCCCTG 
CTGCCTGGAC CCTTGCTGGC GGCTCACCCT CAAGCAGGGA CCAGGCCCTC TGCCAACCAG 
CTTCCCTGGC CCATTCTATG TTCACCAGTG GTTGGGGTGC TTCTCCTGGC CCTTGGGGCC 
CTTCTCGTCC TCCAGCTCAT TCGGCGACGG CGACGAGAAC ATGGGGCCCT GTGGCTGCCC 
CCTGGTTTCA TTCGAAGGCC TCAGACACAG CAGGCACCCC ACCGGCGGAG GCCCCCACTG 
GGCGAGGACA ACATTGGTCT TAAGGCACTG AAGCCAGAGG CCGAAGTGGA TGAGGATGGA 
GTGGCCATGT GCTCGGGCCC TGAAGAGGGA GAGGCTGAAG AAACAGCCTC AGCCTCCAGG 
TGCCAGCTTT GGCCGCTCAA CAGCGGCTGT GGAGAGCTCC CCCAGGCAGC CATGCTGACC 
CCTCCTCAGG AGTGTGAATC GGAGGTTCTG GATGTGGACA CCTGTGGACC TGATGGGGTG 
ACACCCCTGA TGTCAGCCGT CTTCTGTGGG GGAGTGCAGT CCACGACTGG GGCTAGTCCA 
CAGAGACTGG GGCTAGGAAA TCTGGAACCC TGGGAACCAC TGCTGGATAG AGGGGCCTGC 
CCCCAGGCTC ACACTGTGGG CACTGGAGAG ACGCCTCTGC ACCTAGCTGC CAGATTCTCT 
CGGCCAACCG CTGCCCGCCG CCTCCTTGAG GCTGGAGCCA ACCCCAACCA GCCAGACCGC 
GCTGGGCGCA CCCCACTTCA CACTGCTGTG GCTGCCGACG CTCGGGAGGT TTGCCAGCTC 
CTATTGGCCA GCAGACAGAC TACGGTGGAC GCCCGCACAG AGGACGGGAC TACACCTTTG 
ATGCTGGCTG CCAGGCTGGC CGTGGAGGAC CTGGTTGAAG AATTGATCGC AGCCCGAGCA 
GATGTAGGAG CCAGGGATAA AAGGGGAAAA ACTGCACTGC ACTGGGCCGC TGCTGTGAAC 
AACGCCCGAG CCGCCCGCTC TCTCCTCCAG GCTGGAGCGG ATAAAGATGC CCAGGACAGT 
AGGGAACAGA CGCCGCTTTT CCTGGCAGCG CGCGAAGGAG CCGTGGAGGT GGCGCAGCTG 
TTGCTGGAGC TCGGGGCGGC CCGGGGACTG CGAGACCAGG CCGGGCTGGC CCCAGGAGAT 
GTGGCCCGCC AGCGCAGTCA CTGGGACCTG CTAACGCTGC TGGAAGGGGC TGGACCGACT 
ACGCAGGAGG CCCGTGCGCA CGCACGCACC ACGCCGGGGG GCGGGGCCGC GCCGCGCTGC 
CGGACGCTGT CTGCGGGAGC GCGCCCGCGC GGGGGCGGAG CCTGTCTGCA GGCTCGCACT 
TGGTCGGTGG ACTTGGGAGC GCGCGGAGGG AAGGTGTATG CTCGCTGCCG GAGCCGATCT 
GGAAGCTGCG GAGGCCCCAC CACGCGCGGC CGCAGGTTCT CCGCGGGCTC CCGTGGACGA 
CGCGGGGCTA GGGCATCACA GGATGACTGG CCTCGCGACT GGGTGGCCCT GGAAGCCTGC 
GGCTCCGCCT GCAGTGCGCC GATCCCGCCT CCCAGCCTGA CCCCGTCCCC AGAACGTGGA 
TCCCCTCAAG TTGCCTGGGG TCTTCCAGTT CACCAAGAGA TTCCCTTAAA CTCGGTTGTA 
AGAAATCTGA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Notch4 ( XM_006523798.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523861.1 Mus musculus notch 4 (Notch4), transcript variant X1, mRNA.

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CloneID	OMu53249
Clone ID Related Accession (Same CDS sequence)	XM_006523798.4 , XM_006523798.3
Accession Version	XM_006523798.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	neurogenic locus notch homolog protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523798.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCCC AGTTGCTGCT GCTGCTGCTC TTGCCACTCA ATTTCCCTGT CATCCTGACC 
AGAGTCTCTT CTCCAGAGCT TCTGTGTGGA GGATCCCCAG AGCCCTGTGC CAACGGAGGC 
ACCTGCCTGA GGCTATCTCG GGGACAAGGG ATCTGCCAGT GTGCCCCTGG ATTTCTGGGT 
GAGACTTGCC AGTTTCCTGA CCCCTGCAGG GATACCCAAC TCTGCAAGAA TGGTGGCAGC 
TGCCAAGCCC TGCTCCCCAC ACCCCCAAGC TCCCGTAGTC CTACTTCTCC ACTGACCCCT 
CACTTCTCCT GCACCTGCCC CTCTGGCTTC ACCGGTGATC GATGCCAAAC CCATCTGGAA 
GAGCTCTGTC CACCTTCTTT CTGTTCCAAC GGGGGTCACT GCTATGTTCA GGCCTCAGGC 
CGCCCACAGT GCTCCTGCGA GCCTGGGTGG ACAGGTGAGC AATGCCAGCT CCGAGACTTC 
TGCTCAGCCA ACCCCTGTGC CAACGGAGGC GTGTGCCTGG CCACATACCC CCAGATCCAG 
TGCCGCTGTC CACCTGGGTT CGAGGGTCAC ACCTGTGAAC GCGACATCAA CGAGTGCTTC 
CTGGAGCCGG GACCCTGCCC TCAGGGCACC TCCTGCCATA ACACCTTGGG TTCCTACCAG 
TGTCTCTGCC CTGTGGGGCA GGAAGGTCCC CAGTGCAAGC TCAGGAAGGG AGCCTGCCCT 
CCTGGAAGCT GTCTCAATGG GGGCACCTGC CAGCTGGTCC CAGAGGGACA CTCCACCTTT 
CATCTCTGCC TCTGTCCCCC AGGTTTCACG GGGCTGGACT GTGAGATGAA CCCAGATGAC 
TGTGTCAGGC ACCAGTGTCA GAACGGGGCC ACCTGTCTGG ATGGGCTGGA CACCTACACC 
TGCCTCTGCC CCAAGACATG GAAGGGCTGG GACTGCTCTG AAGATATAGA TGAATGTGAA 
GCCCGGGGTC CCCCTCGCTG CAGGAACGGT GGCACCTGCC AGAACACAGC TGGCAGCTTT 
CACTGTGTGT GCGTGAGTGG CTGGGGAGGC GCAGGCTGTG AGGAGAACCT GGATGACTGT 
GCAGCTGCCA CCTGTGCCCC GGGATCCACC TGCATCGACC GTGTGGGCTC CTTCTCCTGC 
CTCTGCCCAC CTGGACGCAC AGGCCTCCTG TGCCACCTGG AAGACATGTG TTTGAGTCAG 
CCGTGCCACG TGAATGCCCA GTGCAGCACC AACCCTCTGA CAGGCTCCAC CCTCTGCATA 
TGCCAGCCTG GCTACTCAGG ATCCACCTGT CACCAAGATC TGGATGAGTG CCAAATGGCC 
CAGCAAGGAC CCAGTCCCTG CGAACATGGC GGCTCCTGCA TCAACACCCC TGGCTCCTTC 
AACTGCCTCT GCCTGCCTGG TTACACGGGC TCCCGCTGTG AAGCTGACCA CAATGAGTGC 
CTGTCACAGC CCTGCCACCC AGGCAGCACC TGCCTGGACC TGCTTGCAAC CTTCCACTGC 
CTCTGCCCAC CAGGCTTGGA AGGGAGGCTC TGTGAGGTGG AGGTCAATGA GTGCACCTCT 
AATCCCTGCC TGAACCAAGC TGCCTGCCAT GACCTGCTCA ACGGCTTCCA GTGCCTCTGC 
CTTCCTGGAT TCACCGGCGC CCGATGTGAG AAAGACATGG ACGAGTGTAG CAGCACCCCC 
TGTGCCAATG GGGGGCGCTG CCGAGACCAG CCTGGAGCCT TCTACTGCGA GTGTCTCCCA 
GGCTTTGAAG GGCCACACTG TGAGAAAGAA GTGGACGAAT GTCTGAGTGA CCCCTGCCCC 
GTGGGAGCCA GCTGCCTTGA TCTCCCCGGA GCATTCTTCT GCCTCTGCCG TCCTGGTTTC 
ACAGGTCAAC TTTGTGAGGT TCCCTTGTGC ACCCCCAACA TGTGCCAACC TGGACAGCAA 
TGCCAAGGTC AGGAACACAG AGCCCCCTGC CTCTGCCCTG ACGGAAGTCC TGGCTGTGTT 
CCTGCCGAGG ACAACTGCCC CTGTCACCAT GGCCATTGCC AGAGATCCTT GTGTGTGTGT 
GATGAGGGCT GGACTGGACC AGAATGCGAG ACAGAACTGG GTGGCTGCAT CTCCACACCC 
TGTGCCCATG GGGGGACCTG CCACCCACAG CCATCTGGCT ACAACTGTAC CTGCCCTGCA 
GGCTACATGG GGTTGACCTG TAGTGAGGAG GTGACAGCTT GTCACTCAGG GCCCTGTCTC 
AATGGTGGCT CCTGCAGCAT CCGTCCTGAG GGCTATTCCT GCACCTGCCT TCCAAGTCAC 
ACAGGTCGCC ACTGCCAGAC TGCCGTGGAC CACTGTGTGT CTGCCTCGTG CCTCAATGGG 
GGTACCTGTG TGAACAAGCC TGGCACTTTC TTCTGCCTCT GTGCCACTGG CTTCCAGGGG 
CTGCACTGTG AGGAGAAGAC TAACCCCAGC TGTGCAGACA GCCCCTGCAG GAACAAGGCA 
ACCTGCCAAG ACACACCTCG AGGGGCCCGC TGCCTCTGCA GCCCTGGCTA TACAGGAAGC 
AGCTGCCAGA CTCTGATAGA CTTGTGTGCC CGGAAGCCCT GTCCACACAC TGCTCGATGC 
CTCCAGAGTG GGCCCTCGTT CCAGTGCCTG TGCCTCCAGG GATGGACAGG GGCTCTCTGT 
GACTTCCCAC TGTCCTGCCA GAAGGCCGCG ATGAGCCAAG GCATAGAGAT CTCTGGCCTG 
TGCCAGAATG GAGGCCTCTG TATTGACACG GGCTCCTCCT ATTTCTGCCG CTGCCCTCCT 
GGATTCCAAG GCAAGTTATG CCAGGATAAT GTGAACCCCT GTGAGCCCAA TCCCTGCCAT 
CACGGGTCTA CCTGTGTGCC TCAGCCCAGT GGCTATGTCT GCCAGTGTGC CCCAGGCTAT 
GAGGGACAGA ACTGCTCAAA AGTACTTGAC GCTTGTCAGT CCCAGCCCTG CCACAACCAC 
GGAACCTGTA CCTCCAGGCC TGGAGGCTTC CACTGTGCCT GCCCTCCAGG CTTCGTGGGA 
CTGCGCTGTG AGGGAGATGT GGATGAGTGT CTGGACCGGC CCTGTCACCC CTCGGGCACT 
GCAGCTTGCC ACTCTTTAGC CAACGCCTTC TACTGCCAGT GTCTGCCTGG GCACACAGGC 
CAGCGGTGTG AGGTGGAGAT GGACCTCTGT CAGAGCCAAC CCTGCTCCAA TGGAGGATCC 
TGTGAGATCA CAACAGGGCC ACCCCCTGGC TTCACCTGTC ACTGCCCCAA GGGTTTTGAA 
GGCCCCACCT GCAGCCACAA AGCCCTTTCC TGCGGCATCC ATCACTGCCA CAATGGAGGC 
CTATGTCTGC CCTCCCCTAA GCCAGGGTCA CCACCACTCT GTGCCTGCCT CAGTGGTTTT 
GGGGGCCCTG ACTGTCTGAC ACCTCCAGCT CCACCGGGCT GCGGTCCCCC CTCACCCTGC 
CTGCACAATG GTACCTGCAC TGAGACCCCT GGGTTGGGCA ACCCGGGCTT TCAATGCACC 
TGCCCTCCTG ACTCTCCAGG GCCCCGGTGT CAAAGGCCAG GGGCAAGTGG GTGTGAGGGC 
CGAGGTGGTG ATGGGACCTG CGATGCTGGC TGCAGTGGCC CAGGAGGAGA CTGGGATGGA 
GGGGACTGTT CCCTGGGGGT CCCAGACCCC TGGAAGGGCT GTCCCCCGCA TTCCCAGTGC 
TGGCTTCTGT TCCGGGACGG ACGGTGTCAC CCGCAGTGTG ACTCTGAGGA GTGTCTCTTT 
GATGGCTACG ACTGTGAAAT CCCTCTAACC TGCATCCCAG CCTATGACCA GTACTGCCGA 
GATCACTTCC ACAACGGGCA CTGTGAGAAA GGCTGCAATA ACGCTGAATG TGGCTGGGAC 
GGGGGAGACT GCAGACCAGA AGGGGAAGAC TCAGAGGGGA GGCCCTCCCT GGCCCTGCTG 
GTGGTGCTGA GGCCCCCAGC CCTGGATCAG CAGCTGCTTG CCCTGGCACG AGTGCTGTCC 
CTGACTCTGA GGGTCGGTCT CTGGGTGAGG AAGGACAGTG AAGGCAGGAA CATGGTGTTC 
CCCTATCCTG GGACCCGGGC CAAAGAGGAG CTGAGTGGAG CTAGGGATTC CTCTTCATGG 
GAAAGACAAG CCCCTCCCAC TCAGCCCCTG GGCAAGGAGA CAGAGTCTCT TGGTGCAGGG 
TTTGTGGTAG TGATGGGAGT GGATCTGTCC CGCTGTGGTC CGGAACATCC TGCGTCCCGC 
TGCCCCTGGG ACTCTGGACT CCTGCTGCGC TTCCTTGCAG CAATGGCAGC AGTGGGAGCT 
CTGGAGCCCC TGCTGCCTGG ACCCTTGCTG GCGGCTCACC CTCAAGCAGG GACCAGGCCC 
TCTGCCAACC AGCTTCCCTG GCCCATTCTA TGTTCACCAG TGGTTGGGGT GCTTCTCCTG 
GCCCTTGGGG CCCTTCTCGT CCTCCAGCTC ATTCGGCGAC GGCGACGAGA ACATGGGGCC 
CTGTGGCTGC CCCCTGGTTT CATTCGAAGG CCTCAGACAC AGCAGGCACC CCACCGGCGG 
AGGCCCCCAC TGGGCGAGGA CAACATTGGT CTTAAGGCAC TGAAGCCAGA GGCCGAAGTG 
GATGAGGATG GAGTGGCCAT GTGCTCGGGC CCTGAAGAGG GAGAGGCTGA AGAAACAGCC 
TCAGCCTCCA GGTGCCAGCT TTGGCCGCTC AACAGCGGCT GTGGAGAGCT CCCCCAGGCA 
GCCATGCTGA CCCCTCCTCA GGAGTGTGAA TCGGAGGTTC TGGATGTGGA CACCTGTGGA 
CCTGATGGGG TGACACCCCT GATGTCAGCC GTCTTCTGTG GGGGAGTGCA GTCCACGACT 
GGGGCTAGTC CACAGAGACT GGGGCTAGGA AATCTGGAAC CCTGGGAACC ACTGCTGGAT 
AGAGGGGCCT GCCCCCAGGC TCACACTGTG GGCACTGGAG AGACGCCTCT GCACCTAGCT 
GCCAGATTCT CTCGGCCAAC CGCTGCCCGC CGCCTCCTTG AGGCTGGAGC CAACCCCAAC 
CAGCCAGACC GCGCTGGGCG CACCCCACTT CACACTGCTG TGGCTGCCGA CGCTCGGGAG 
GTTTGCCAGC TCCTATTGGC CAGCAGACAG ACTACGGTGG ACGCCCGCAC AGAGGACGGG 
ACTACACCTT TGATGCTGGC TGCCAGGCTG GCCGTGGAGG ACCTGGTTGA AGAATTGATC 
GCAGCCCGAG CAGATGTAGG AGCCAGGGAT AAAAGGGGAA AAACTGCACT GCACTGGGCC 
GCTGCTGTGA ACAACGCCCG AGCCGCCCGC TCTCTCCTCC AGGCTGGAGC GGATAAAGAT 
GCCCAGGACA GTAGGGAACA GACGCCGCTT TTCCTGGCAG CGCGCGAAGG AGCCGTGGAG 
GTGGCGCAGC TGTTGCTGGA GCTCGGGGCG GCCCGGGGAC TGCGAGACCA GGCCGGGCTG 
GCCCCAGGAG ATGTGGCCCG CCAGCGCAGT CACTGGGACC TGCTAACGCT GCTGGAAGGG 
GCTGGACCGA CTACGCAGGA GGCCCGTGCG CACGCACGCA CCACGCCGGG GGGCGGGGCC 
GCGCCGCGCT GCCGGACGCT GTCTGCGGGA GCGCGCCCGC GCGGGGGCGG AGCCTGTCTG 
CAGGCTCGCA CTTGGTCGGT GGACTTGGGA GCGCGCGGAG GGAAGGTGTA TGCTCGCTGC 
CGGAGCCGAT CTGGAAGCTG CGGAGGCCCC ACCACGCGCG GCCGCAGGTT CTCCGCGGGC 
TCCCGTGGAC GACGCGGGGC TAGGGCATCA CAGGATGACT GGCCTCGCGA CTGGGTGGCC 
CTGGAAGCCT GCGGCTCCGC CTGCAGTGCG CCGATCCCGC CTCCCAGCCT GACCCCGTCC 
CCAGAACGTG GATCCCCTCA AGTTGCCTGG GGTCTTCCAG TTCACCAAGA GATTCCCTTA 
AACTCGGTTG TAAGAAATCT GAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523861.1
CDS	167..6073
Translation	MQPQLLLLLLLPLNFPVILTRVSSPELLCGGSPEPCANGGTCLRLSRGQGICQCAPGFLGETCQFPDPCRDTQLCKNGGSCQALLPTPPSSRSPTSPLTPHFSCTCPSGFTGDRCQTHLEELCPPSFCSNGGHCYVQASGRPQCSCEPGWTGEQCQLRDFCSANPCANGGVCLATYPQIQCRCPPGFEGHTCERDINECFLEPGPCPQGTSCHNTLGSYQCLCPVGQEGPQCKLRKGACPPGSCLNGGTCQLVPEGHSTFHLCLCPPGFTGLDCEMNPDDCVRHQCQNGATCLDGLDTYTCLCPKTWKGWDCSEDIDECEARGPPRCRNGGTCQNTAGSFHCVCVSGWGGAGCEENLDDCAAATCAPGSTCIDRVGSFSCLCPPGRTGLLCHLEDMCLSQPCHVNAQCSTNPLTGSTLCICQPGYSGSTCHQDLDECQMAQQGPSPCEHGGSCINTPGSFNCLCLPGYTGSRCEADHNECLSQPCHPGSTCLDLLATFHCLCPPGLEGRLCEVEVNECTSNPCLNQAACHDLLNGFQCLCLPGFTGARCEKDMDECSSTPCANGGRCRDQPGAFYCECLPGFEGPHCEKEVDECLSDPCPVGASCLDLPGAFFCLCRPGFTGQLCEVPLCTPNMCQPGQQCQGQEHRAPCLCPDGSPGCVPAEDNCPCHHGHCQRSLCVCDEGWTGPECETELGGCISTPCAHGGTCHPQPSGYNCTCPAGYMGLTCSEEVTACHSGPCLNGGSCSIRPEGYSCTCLPSHTGRHCQTAVDHCVSASCLNGGTCVNKPGTFFCLCATGFQGLHCEEKTNPSCADSPCRNKATCQDTPRGARCLCSPGYTGSSCQTLIDLCARKPCPHTARCLQSGPSFQCLCLQGWTGALCDFPLSCQKAAMSQGIEISGLCQNGGLCIDTGSSYFCRCPPGFQGKLCQDNVNPCEPNPCHHGSTCVPQPSGYVCQCAPGYEGQNCSKVLDACQSQPCHNHGTCTSRPGGFHCACPPGFVGLRCEGDVDECLDRPCHPSGTAACHSLANAFYCQCLPGHTGQRCEVEMDLCQSQPCSNGGSCEITTGPPPGFTCHCPKGFEGPTCSHKALSCGIHHCHNGGLCLPSPKPGSPPLCACLSGFGGPDCLTPPAPPGCGPPSPCLHNGTCTETPGLGNPGFQCTCPPDSPGPRCQRPGASGCEGRGGDGTCDAGCSGPGGDWDGGDCSLGVPDPWKGCPPHSQCWLLFRDGRCHPQCDSEECLFDGYDCEIPLTCIPAYDQYCRDHFHNGHCEKGCNNAECGWDGGDCRPEGEDSEGRPSLALLVVLRPPALDQQLLALARVLSLTLRVGLWVRKDSEGRNMVFPYPGTRAKEELSGARDSSSWERQAPPTQPLGKETESLGAGFVVVMGVDLSRCGPEHPASRCPWDSGLLLRFLAAMAAVGALEPLLPGPLLAAHPQAGTRPSANQLPWPILCSPVVGVLLLALGALLVLQLIRRRRREHGALWLPPGFIRRPQTQQAPHRRRPPLGEDNIGLKALKPEAEVDEDGVAMCSGPEEGEAEETASASRCQLWPLNSGCGELPQAAMLTPPQECESEVLDVDTCGPDGVTPLMSAVFCGGVQSTTGASPQRLGLGNLEPWEPLLDRGACPQAHTVGTGETPLHLAARFSRPTAARRLLEAGANPNQPDRAGRTPLHTAVAADAREVCQLLLASRQTTVDARTEDGTTPLMLAARLAVEDLVEELIAARADVGARDKRGKTALHWAAAVNNARAARSLLQAGADKDAQDSREQTPLFLAAREGAVEVAQLLLELGAARGLRDQAGLAPGDVARQRSHWDLLTLLEGAGPTTQEARAHARTTPGGGAAPRCRTLSAGARPRGGGACLQARTWSVDLGARGGKVYARCRSRSGSCGGPTTRGRRFSAGSRGRRGARASQDDWPRDWVALEACGSACSAPIPPPSLTPSPERGSPQVAWGLPVHQEIPLNSVVRNLN

Target ORF information:

RefSeq Version	XM_006523798.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus notch 4 (Notch4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523798.3

ORF Insert Sequence:

ATGCAGCCCC AGTTGCTGCT GCTGCTGCTC TTGCCACTCA ATTTCCCTGT CATCCTGACC 
AGAGTCTCTT CTCCAGAGCT TCTGTGTGGA GGATCCCCAG AGCCCTGTGC CAACGGAGGC 
ACCTGCCTGA GGCTATCTCG GGGACAAGGG ATCTGCCAGT GTGCCCCTGG ATTTCTGGGT 
GAGACTTGCC AGTTTCCTGA CCCCTGCAGG GATACCCAAC TCTGCAAGAA TGGTGGCAGC 
TGCCAAGCCC TGCTCCCCAC ACCCCCAAGC TCCCGTAGTC CTACTTCTCC ACTGACCCCT 
CACTTCTCCT GCACCTGCCC CTCTGGCTTC ACCGGTGATC GATGCCAAAC CCATCTGGAA 
GAGCTCTGTC CACCTTCTTT CTGTTCCAAC GGGGGTCACT GCTATGTTCA GGCCTCAGGC 
CGCCCACAGT GCTCCTGCGA GCCTGGGTGG ACAGGTGAGC AATGCCAGCT CCGAGACTTC 
TGCTCAGCCA ACCCCTGTGC CAACGGAGGC GTGTGCCTGG CCACATACCC CCAGATCCAG 
TGCCGCTGTC CACCTGGGTT CGAGGGTCAC ACCTGTGAAC GCGACATCAA CGAGTGCTTC 
CTGGAGCCGG GACCCTGCCC TCAGGGCACC TCCTGCCATA ACACCTTGGG TTCCTACCAG 
TGTCTCTGCC CTGTGGGGCA GGAAGGTCCC CAGTGCAAGC TCAGGAAGGG AGCCTGCCCT 
CCTGGAAGCT GTCTCAATGG GGGCACCTGC CAGCTGGTCC CAGAGGGACA CTCCACCTTT 
CATCTCTGCC TCTGTCCCCC AGGTTTCACG GGGCTGGACT GTGAGATGAA CCCAGATGAC 
TGTGTCAGGC ACCAGTGTCA GAACGGGGCC ACCTGTCTGG ATGGGCTGGA CACCTACACC 
TGCCTCTGCC CCAAGACATG GAAGGGCTGG GACTGCTCTG AAGATATAGA TGAATGTGAA 
GCCCGGGGTC CCCCTCGCTG CAGGAACGGT GGCACCTGCC AGAACACAGC TGGCAGCTTT 
CACTGTGTGT GCGTGAGTGG CTGGGGAGGC GCAGGCTGTG AGGAGAACCT GGATGACTGT 
GCAGCTGCCA CCTGTGCCCC GGGATCCACC TGCATCGACC GTGTGGGCTC CTTCTCCTGC 
CTCTGCCCAC CTGGACGCAC AGGCCTCCTG TGCCACCTGG AAGACATGTG TTTGAGTCAG 
CCGTGCCACG TGAATGCCCA GTGCAGCACC AACCCTCTGA CAGGCTCCAC CCTCTGCATA 
TGCCAGCCTG GCTACTCAGG ATCCACCTGT CACCAAGATC TGGATGAGTG CCAAATGGCC 
CAGCAAGGAC CCAGTCCCTG CGAACATGGC GGCTCCTGCA TCAACACCCC TGGCTCCTTC 
AACTGCCTCT GCCTGCCTGG TTACACGGGC TCCCGCTGTG AAGCTGACCA CAATGAGTGC 
CTGTCACAGC CCTGCCACCC AGGCAGCACC TGCCTGGACC TGCTTGCAAC CTTCCACTGC 
CTCTGCCCAC CAGGCTTGGA AGGGAGGCTC TGTGAGGTGG AGGTCAATGA GTGCACCTCT 
AATCCCTGCC TGAACCAAGC TGCCTGCCAT GACCTGCTCA ACGGCTTCCA GTGCCTCTGC 
CTTCCTGGAT TCACCGGCGC CCGATGTGAG AAAGACATGG ACGAGTGTAG CAGCACCCCC 
TGTGCCAATG GGGGGCGCTG CCGAGACCAG CCTGGAGCCT TCTACTGCGA GTGTCTCCCA 
GGCTTTGAAG GGCCACACTG TGAGAAAGAA GTGGACGAAT GTCTGAGTGA CCCCTGCCCC 
GTGGGAGCCA GCTGCCTTGA TCTCCCCGGA GCATTCTTCT GCCTCTGCCG TCCTGGTTTC 
ACAGGTCAAC TTTGTGAGGT TCCCTTGTGC ACCCCCAACA TGTGCCAACC TGGACAGCAA 
TGCCAAGGTC AGGAACACAG AGCCCCCTGC CTCTGCCCTG ACGGAAGTCC TGGCTGTGTT 
CCTGCCGAGG ACAACTGCCC CTGTCACCAT GGCCATTGCC AGAGATCCTT GTGTGTGTGT 
GATGAGGGCT GGACTGGACC AGAATGCGAG ACAGAACTGG GTGGCTGCAT CTCCACACCC 
TGTGCCCATG GGGGGACCTG CCACCCACAG CCATCTGGCT ACAACTGTAC CTGCCCTGCA 
GGCTACATGG GGTTGACCTG TAGTGAGGAG GTGACAGCTT GTCACTCAGG GCCCTGTCTC 
AATGGTGGCT CCTGCAGCAT CCGTCCTGAG GGCTATTCCT GCACCTGCCT TCCAAGTCAC 
ACAGGTCGCC ACTGCCAGAC TGCCGTGGAC CACTGTGTGT CTGCCTCGTG CCTCAATGGG 
GGTACCTGTG TGAACAAGCC TGGCACTTTC TTCTGCCTCT GTGCCACTGG CTTCCAGGGG 
CTGCACTGTG AGGAGAAGAC TAACCCCAGC TGTGCAGACA GCCCCTGCAG GAACAAGGCA 
ACCTGCCAAG ACACACCTCG AGGGGCCCGC TGCCTCTGCA GCCCTGGCTA TACAGGAAGC 
AGCTGCCAGA CTCTGATAGA CTTGTGTGCC CGGAAGCCCT GTCCACACAC TGCTCGATGC 
CTCCAGAGTG GGCCCTCGTT CCAGTGCCTG TGCCTCCAGG GATGGACAGG GGCTCTCTGT 
GACTTCCCAC TGTCCTGCCA GAAGGCCGCG ATGAGCCAAG GCATAGAGAT CTCTGGCCTG 
TGCCAGAATG GAGGCCTCTG TATTGACACG GGCTCCTCCT ATTTCTGCCG CTGCCCTCCT 
GGATTCCAAG GCAAGTTATG CCAGGATAAT GTGAACCCCT GTGAGCCCAA TCCCTGCCAT 
CACGGGTCTA CCTGTGTGCC TCAGCCCAGT GGCTATGTCT GCCAGTGTGC CCCAGGCTAT 
GAGGGACAGA ACTGCTCAAA AGTACTTGAC GCTTGTCAGT CCCAGCCCTG CCACAACCAC 
GGAACCTGTA CCTCCAGGCC TGGAGGCTTC CACTGTGCCT GCCCTCCAGG CTTCGTGGGA 
CTGCGCTGTG AGGGAGATGT GGATGAGTGT CTGGACCGGC CCTGTCACCC CTCGGGCACT 
GCAGCTTGCC ACTCTTTAGC CAACGCCTTC TACTGCCAGT GTCTGCCTGG GCACACAGGC 
CAGCGGTGTG AGGTGGAGAT GGACCTCTGT CAGAGCCAAC CCTGCTCCAA TGGAGGATCC 
TGTGAGATCA CAACAGGGCC ACCCCCTGGC TTCACCTGTC ACTGCCCCAA GGGTTTTGAA 
GGCCCCACCT GCAGCCACAA AGCCCTTTCC TGCGGCATCC ATCACTGCCA CAATGGAGGC 
CTATGTCTGC CCTCCCCTAA GCCAGGGTCA CCACCACTCT GTGCCTGCCT CAGTGGTTTT 
GGGGGCCCTG ACTGTCTGAC ACCTCCAGCT CCACCGGGCT GCGGTCCCCC CTCACCCTGC 
CTGCACAATG GTACCTGCAC TGAGACCCCT GGGTTGGGCA ACCCGGGCTT TCAATGCACC 
TGCCCTCCTG ACTCTCCAGG GCCCCGGTGT CAAAGGCCAG GGGCAAGTGG GTGTGAGGGC 
CGAGGTGGTG ATGGGACCTG CGATGCTGGC TGCAGTGGCC CAGGAGGAGA CTGGGATGGA 
GGGGACTGTT CCCTGGGGGT CCCAGACCCC TGGAAGGGCT GTCCCCCGCA TTCCCAGTGC 
TGGCTTCTGT TCCGGGACGG ACGGTGTCAC CCGCAGTGTG ACTCTGAGGA GTGTCTCTTT 
GATGGCTACG ACTGTGAAAT CCCTCTAACC TGCATCCCAG CCTATGACCA GTACTGCCGA 
GATCACTTCC ACAACGGGCA CTGTGAGAAA GGCTGCAATA ACGCTGAATG TGGCTGGGAC 
GGGGGAGACT GCAGACCAGA AGGGGAAGAC TCAGAGGGGA GGCCCTCCCT GGCCCTGCTG 
GTGGTGCTGA GGCCCCCAGC CCTGGATCAG CAGCTGCTTG CCCTGGCACG AGTGCTGTCC 
CTGACTCTGA GGGTCGGTCT CTGGGTGAGG AAGGACAGTG AAGGCAGGAA CATGGTGTTC 
CCCTATCCTG GGACCCGGGC CAAAGAGGAG CTGAGTGGAG CTAGGGATTC CTCTTCATGG 
GAAAGACAAG CCCCTCCCAC TCAGCCCCTG GGCAAGGAGA CAGAGTCTCT TGGTGCAGGG 
TTTGTGGTAG TGATGGGAGT GGATCTGTCC CGCTGTGGTC CGGAACATCC TGCGTCCCGC 
TGCCCCTGGG ACTCTGGACT CCTGCTGCGC TTCCTTGCAG CAATGGCAGC AGTGGGAGCT 
CTGGAGCCCC TGCTGCCTGG ACCCTTGCTG GCGGCTCACC CTCAAGCAGG GACCAGGCCC 
TCTGCCAACC AGCTTCCCTG GCCCATTCTA TGTTCACCAG TGGTTGGGGT GCTTCTCCTG 
GCCCTTGGGG CCCTTCTCGT CCTCCAGCTC ATTCGGCGAC GGCGACGAGA ACATGGGGCC 
CTGTGGCTGC CCCCTGGTTT CATTCGAAGG CCTCAGACAC AGCAGGCACC CCACCGGCGG 
AGGCCCCCAC TGGGCGAGGA CAACATTGGT CTTAAGGCAC TGAAGCCAGA GGCCGAAGTG 
GATGAGGATG GAGTGGCCAT GTGCTCGGGC CCTGAAGAGG GAGAGGCTGA AGAAACAGCC 
TCAGCCTCCA GGTGCCAGCT TTGGCCGCTC AACAGCGGCT GTGGAGAGCT CCCCCAGGCA 
GCCATGCTGA CCCCTCCTCA GGAGTGTGAA TCGGAGGTTC TGGATGTGGA CACCTGTGGA 
CCTGATGGGG TGACACCCCT GATGTCAGCC GTCTTCTGTG GGGGAGTGCA GTCCACGACT 
GGGGCTAGTC CACAGAGACT GGGGCTAGGA AATCTGGAAC CCTGGGAACC ACTGCTGGAT 
AGAGGGGCCT GCCCCCAGGC TCACACTGTG GGCACTGGAG AGACGCCTCT GCACCTAGCT 
GCCAGATTCT CTCGGCCAAC CGCTGCCCGC CGCCTCCTTG AGGCTGGAGC CAACCCCAAC 
CAGCCAGACC GCGCTGGGCG CACCCCACTT CACACTGCTG TGGCTGCCGA CGCTCGGGAG 
GTTTGCCAGC TCCTATTGGC CAGCAGACAG ACTACGGTGG ACGCCCGCAC AGAGGACGGG 
ACTACACCTT TGATGCTGGC TGCCAGGCTG GCCGTGGAGG ACCTGGTTGA AGAATTGATC 
GCAGCCCGAG CAGATGTAGG AGCCAGGGAT AAAAGGGGAA AAACTGCACT GCACTGGGCC 
GCTGCTGTGA ACAACGCCCG AGCCGCCCGC TCTCTCCTCC AGGCTGGAGC GGATAAAGAT 
GCCCAGGACA GTAGGGAACA GACGCCGCTT TTCCTGGCAG CGCGCGAAGG AGCCGTGGAG 
GTGGCGCAGC TGTTGCTGGA GCTCGGGGCG GCCCGGGGAC TGCGAGACCA GGCCGGGCTG 
GCCCCAGGAG ATGTGGCCCG CCAGCGCAGT CACTGGGACC TGCTAACGCT GCTGGAAGGG 
GCTGGACCGA CTACGCAGGA GGCCCGTGCG CACGCACGCA CCACGCCGGG GGGCGGGGCC 
GCGCCGCGCT GCCGGACGCT GTCTGCGGGA GCGCGCCCGC GCGGGGGCGG AGCCTGTCTG 
CAGGCTCGCA CTTGGTCGGT GGACTTGGGA GCGCGCGGAG GGAAGGTGTA TGCTCGCTGC 
CGGAGCCGAT CTGGAAGCTG CGGAGGCCCC ACCACGCGCG GCCGCAGGTT CTCCGCGGGC 
TCCCGTGGAC GACGCGGGGC TAGGGCATCA CAGGATGACT GGCCTCGCGA CTGGGTGGCC 
CTGGAAGCCT GCGGCTCCGC CTGCAGTGCG CCGATCCCGC CTCCCAGCCT GACCCCGTCC 
CCAGAACGTG GATCCCCTCA AGTTGCCTGG GGTCTTCCAG TTCACCAAGA GATTCCCTTA 
AACTCGGTTG TAAGAAATCT GAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Notch4 Negatively Regulates the Inflammatory Response to Mycobacterium tuberculosis Infection by Inhibiting TAK1 Activation.
The Journal of infectious diseases(2017 Dec)
Zheng R,Liu H,Zhou Y,Yan D,Chen J,Ma D,Feng Y,Qin L,Liu F,Huang X,Wang J,Ge B

The ANK repeats of Notch-4/Int3 activate NF-?B canonical pathway in the absence of Rbpj and causes mammary tumorigenesis.
Scientific reports7(1)13690(2017 Oct)
Raafat A,Bargo S,McCurdy D,Callahan R

Uncontrolled angiogenic precursor expansion causes coronary artery anomalies in mice lacking Pofut1.
Nature communications8(1)578(2017 09)
Wang Y,Wu B,Lu P,Zhang D,Wu B,Varshney S,Del Monte-Nieto G,Zhuang Z,Charafeddine R,Kramer AH,Sibinga NE,Frangogiannis NG,Kitsis RN,Adams RH,Alitalo K,Sharp DJ,Harvey RP,Stanley P,Zhou B

Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling.
Nature communications8(1)413(2017 09)
Liu M,Liang K,Zhen J,Zhou M,Wang X,Wang Z,Wei X,Zhang Y,Sun Y,Zhou Z,Su H,Zhang C,Li N,Gao C,Peng J,Yi F

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Notch4 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

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ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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