Poln cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Poln
Entrez Gene ID	272158
Full Name	DNA polymerase N
Synonyms	POL4P
General protein information	Preferred Names DNA polymerase N Names DNA polymerase nu DNA-directed DNA polymerase nu
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001289803.1	NP_001276732.1	DNA polymerase nu isoform 2
NM_001289804.1	NP_001276733.1	DNA polymerase nu isoform 3
NM_181857.4	NP_862905.1	DNA polymerase nu isoform 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Poln	NP_862905.1
Gallus gallus (chicken)	POLN	XP_420834.4
	LOC722353	XP_002802209.1
Rattus norvegicus (Norway rat)	LOC102555079	XP_006251474.1
Pan troglodytes (chimpanzee)	POLN	XP_001147379.2
Bos taurus (cattle)	POLN	XP_002688485.3
Canis lupus familiaris (dog)	POLN	XP_005618580.1

Gene Ontology
Bibliography

Related articles in PubMed

Analysis of DNA polymerase ? function in meiotic recombination, immunoglobulin class-switching, and DNA damage tolerance.
Takata KI, Reh S, Yousefzadeh MJ, Zelazowski MJ, Bhetawal S, Trono D, Lowery MG, Sandoval M, Takata Y, Lu Y, Lin K, Shen J, Kusewitt DF, McBride KM, Cole F, Wood RD
PLoS genetics13(6)e1006818(2017 Jun)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Ubiquitin ligase Rad18Sc localizes to the XY body and to other chromosomal regions that are unpaired and transcriptionally silenced during male meiotic prophase.
van der Laan R, Uringa EJ, Wassenaar E, Hoogerbrugge JW, Sleddens E, Odijk H, Roest HP, de Boer P, Hoeijmakers JH, Grootegoed JA, Baarends WM
Journal of cell science117(Pt 21)5023-33(2004 Oct)

POLN, a nuclear PolA family DNA polymerase homologous to the DNA cross-link sensitivity protein Mus308.
Marini F, Kim N, Schuffert A, Wood RD
The Journal of biological chemistry278(34)32014-9(2003 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Inactivation of Poln in mouse embryonic fibroblasts did not alter cellular sensitivity to mitomycin C, cisplatin, or aldehydes. Pol nu-defective mice had no alteration in DNA end-joining during immunoglobulin class-switching.
Title: Analysis of DNA polymerase ? function in meiotic recombination, immunoglobulin class-switching, and DNA damage tolerance.

identification as homolog to Mus308
Title: POLN, a nuclear PolA family DNA polymerase homologous to the DNA cross-link sensitivity protein Mus308.

The following Poln gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Poln cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu58325	NM_001289803.1 Latest version!	Mus musculus DNA polymerase N (Poln), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu58328	NM_001289804.1 Latest version!	Mus musculus DNA polymerase N (Poln), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu58329	NM_181857.4 Latest version!	Mus musculus DNA polymerase N (Poln), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Poln ( NM_001289803.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001276732.1 Mus musculus DNA polymerase N (Poln), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu58325
Clone ID Related Accession (Same CDS sequence)	NM_001289803.1
Accession Version	NM_001289803.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2547bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Mus musculus(house mouse)
Product	DNA polymerase nu isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ083086.1, AK133063.1, AC107709.12 and BY585631.1. Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. It encodes isoform 2, which lacks an internal segment and is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC145378.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGAAAATGG AAAATTATGA GGCATGTGTA GGTTTTGATG TCTGTGAGAT ACCCCTCTCT 
GCTGTTGCTC AGAAGATTAT GTCTGCTATG CGTTCAGGTG ATTTCATGGA TTCTAGGAAT 
GAGGGAGAGA GTACAAACAC TTCAAAAGTG GCAAAGAAAT CCAGTGTTCA TTACTCAGTA 
TTAGCTGAAC ATGAGGAGAC TCAATCACTA GGAACAAAGA ACCCCGAGTC ATTAATAACC 
CAGACACCAA GAGGATCCAT TGAGCTCTGT CCTCAGCCCT CCATTACTAA GCTCACATGT 
CAGCTCTCTG CTGGTCAAGT ACAGAACAGC ATCAGCTCAT TGGGTCTTTC CAGTTATTTA 
ATTCCACAGT GTGATCAGGA AGCTTCAGTT TTACCGAACA TGGAGCATAA GAGACAGCAT 
TTCCTAAAGG AAAATATAGG CAAAGAAGAT AAGGACAACT CGAGTCTTAA AAGAAAATAT 
ATTACATGTA GTAAATCCTC AGAAAAAGCA AGTAAACACA CAGCTCTGGA AAAAGATACT 
GATGGGACTG AATCCTGGCC AAACTCCAGG GACACAAGAG CTTTGGGGGA AAGGCTGTGT 
GATGTTAGAT ATTTGGGTGA TTTGGCCAAA GCCCAGCTGA TGGATGCTCT CAAGCAGGCA 
GCAGCCCTGG TGGTGACATT GATGTACAAG GATGGTTCCA CACAGCTGAG CGCCAAGGAG 
GCTTTAACTT GTACTGTTAA AGGAATTGTA GTGTTACTAA AAAGCCATGT AGGCAACAGC 
ACTCTGACTC TCCCAGCCCA TGGTGGTGCT CTGGAAAAGG ACTTCATATC CGAAGATCAC 
TGTGTCTACA TACATACGGA GCATTCCCCT TTCTGGGACC CAAAGCAAGA AGCACATAGT 
CTATTTGTCA GGAACATACT GTTTTGGACA CTGAGGTGTA AATGTCCCGT TGTTTGTTTT 
AATGCCAAGG ACTTTGTGAG AACAGTGCTG CAGCTCTATG GTGAAGATGG CAGTTGGAAG 
CATGTTGCTG ATTTTGTAGG ACTAGATCCC AGAGTTGCTG CATGGCTCAT AGACCCCAGT 
GACACTGCAC CTTCATTTGA AGATTTAGTA GCAAAACACC TAGAAAAGTC CATCACAGTC 
AAACCAAGCA GCACATTCAG AGAAGCCTCA AGAAATACTT TGAGTCAGAA TGTATTTATG 
AATCTGAAGA TACTCTACGA CCTTACAATG GATCTTTGTT CTAAGTTGAA GGCTTATGGC 
TTATGGCAGC TATTTTGCAC TTTGGAGCTT CCATTGATAC CAATTTTGGC AGTGATGGAA 
AACCATAAGA TTCCAGTTGA CAAAGAAGAA ATGGAGAGGA CATCGGCACT CCTCGGGGCT 
CGCCTCAAGG AATTGGAGCA AGAAGCCCAT TTTGTTGCAG GAGAACAGTT TCTTATAATG 
AGTAATAATC AACTTCGTGA GATCCTCTTT GGCAAGTTGA AGCTGCACTT GTTGAGTCAG 
AGGAAACATC TTCCCAGAAC TGGGCTGCAG AACCAGCTGT CCACATCAGA AGCCATGTTA 
AATTCTCTAC AAGACCTTCA CCCATTACCC AAGTTAATTT TGGAATATAG ACAGGGCTCC 
ATCTCTTCTA CATGGAATCA GACTGGAACT GTGACAGGGA GACTTTCAGC CAAGCATCCA 
AATATCCAGG GTATCTCCAA GCACCCAATT AAGATTTCTA AGCCTTGGAA TTTTAAAGGT 
AAAGAAGAGG AGACTGTCAC CATCTCACCA AGAACCCTGT TTGTGTCCTC TGAAGGCCAC 
ACCTTCCTGG CAGCAGACTT TTCACAGATT GAATTGCGCA TTCTTGCACA TTTATCTGGG 
GATCCAGAGC TTTTGAAGTT ATTCCAGGAA TCTGAAAGAG ATGATGTATT TTCTACCCTG 
ACTTCACAGT GGAAGGACAT TCCCATAGAG CGTGTGACAC ACATGGACAG AGAACAAACC 
AAGAAAGTGG TGTACTCCGT GGTCTATGGA GCTGGGAAAG AGCGGCTTGC TGCTTGCCTT 
GGAGTCACTG TTTTGGAAGC CACCCACTTT TTGGAGAGAT TTTTGCAGAA GTACAAGAAA 
ATCAAGGACT TTGCCCAGAC AGTTATTGGC CAGTGTCACA GTGCAGGCTA TGTGACATCC 
ATCTTGGGTA GAAGGAGGCC TCTCCCGAGG ATCTGTGCAC AGGACCAGCA GCTTCGGGCA 
CAAGCAGAGC GACAGGCAGT AAACTTTGTG GTACAAGGCT CAGCCGCAGA CCTCTGTAAG 
CTAGCCATGA TTCGCATCTC TACTGCAGTA GCCACATCCC CGACCTTGAC AGCCAGGTTA 
GTTGCCCAAA TTCATGATGA GCTGCTGTTT GAAGTAGAAG ACACTCAGGT CCCTGAGTTT 
GCAGCTCTTG TCAGAAGGAT CATGGAGTCC TTGCAACAGG TACAGACCCT GGAACTGCAG 
CTGCAGGTGC CCCTAAAAGT GAACTTGAGT GTTGGCCGCT CATGGGGACA CCTGACCCCA 
CTGCAGGAGA TCCTGGGCTC AGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276732.1
CDS	144..2690
Translation	MKMENYEACVGFDVCEIPLSAVAQKIMSAMRSGDFMDSRNEGESTNTSKVAKKSSVHYSVLAEHEETQSLGTKNPESLITQTPRGSIELCPQPSITKLTCQLSAGQVQNSISSLGLSSYLIPQCDQEASVLPNMEHKRQHFLKENIGKEDKDNSSLKRKYITCSKSSEKASKHTALEKDTDGTESWPNSRDTRALGERLCDVRYLGDLAKAQLMDALKQAAALVVTLMYKDGSTQLSAKEALTCTVKGIVVLLKSHVGNSTLTLPAHGGALEKDFISEDHCVYIHTEHSPFWDPKQEAHSLFVRNILFWTLRCKCPVVCFNAKDFVRTVLQLYGEDGSWKHVADFVGLDPRVAAWLIDPSDTAPSFEDLVAKHLEKSITVKPSSTFREASRNTLSQNVFMNLKILYDLTMDLCSKLKAYGLWQLFCTLELPLIPILAVMENHKIPVDKEEMERTSALLGARLKELEQEAHFVAGEQFLIMSNNQLREILFGKLKLHLLSQRKHLPRTGLQNQLSTSEAMLNSLQDLHPLPKLILEYRQGSISSTWNQTGTVTGRLSAKHPNIQGISKHPIKISKPWNFKGKEEETVTISPRTLFVSSEGHTFLAADFSQIELRILAHLSGDPELLKLFQESERDDVFSTLTSQWKDIPIERVTHMDREQTKKVVYSVVYGAGKERLAACLGVTVLEATHFLERFLQKYKKIKDFAQTVIGQCHSAGYVTSILGRRRPLPRICAQDQQLRAQAERQAVNFVVQGSAADLCKLAMIRISTAVATSPTLTARLVAQIHDELLFEVEDTQVPEFAALVRRIMESLQQVQTLELQLQVPLKVNLSVGRSWGHLTPLQEILGSA

Target ORF information:

RefSeq Version	NM_001289803.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DNA polymerase N (Poln), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289803.1

ORF Insert Sequence:

ATGAAAATGG AAAATTATGA GGCATGTGTA GGTTTTGATG TCTGTGAGAT ACCCCTCTCT 
GCTGTTGCTC AGAAGATTAT GTCTGCTATG CGTTCAGGTG ATTTCATGGA TTCTAGGAAT 
GAGGGAGAGA GTACAAACAC TTCAAAAGTG GCAAAGAAAT CCAGTGTTCA TTACTCAGTA 
TTAGCTGAAC ATGAGGAGAC TCAATCACTA GGAACAAAGA ACCCCGAGTC ATTAATAACC 
CAGACACCAA GAGGATCCAT TGAGCTCTGT CCTCAGCCCT CCATTACTAA GCTCACATGT 
CAGCTCTCTG CTGGTCAAGT ACAGAACAGC ATCAGCTCAT TGGGTCTTTC CAGTTATTTA 
ATTCCACAGT GTGATCAGGA AGCTTCAGTT TTACCGAACA TGGAGCATAA GAGACAGCAT 
TTCCTAAAGG AAAATATAGG CAAAGAAGAT AAGGACAACT CGAGTCTTAA AAGAAAATAT 
ATTACATGTA GTAAATCCTC AGAAAAAGCA AGTAAACACA CAGCTCTGGA AAAAGATACT 
GATGGGACTG AATCCTGGCC AAACTCCAGG GACACAAGAG CTTTGGGGGA AAGGCTGTGT 
GATGTTAGAT ATTTGGGTGA TTTGGCCAAA GCCCAGCTGA TGGATGCTCT CAAGCAGGCA 
GCAGCCCTGG TGGTGACATT GATGTACAAG GATGGTTCCA CACAGCTGAG CGCCAAGGAG 
GCTTTAACTT GTACTGTTAA AGGAATTGTA GTGTTACTAA AAAGCCATGT AGGCAACAGC 
ACTCTGACTC TCCCAGCCCA TGGTGGTGCT CTGGAAAAGG ACTTCATATC CGAAGATCAC 
TGTGTCTACA TACATACGGA GCATTCCCCT TTCTGGGACC CAAAGCAAGA AGCACATAGT 
CTATTTGTCA GGAACATACT GTTTTGGACA CTGAGGTGTA AATGTCCCGT TGTTTGTTTT 
AATGCCAAGG ACTTTGTGAG AACAGTGCTG CAGCTCTATG GTGAAGATGG CAGTTGGAAG 
CATGTTGCTG ATTTTGTAGG ACTAGATCCC AGAGTTGCTG CATGGCTCAT AGACCCCAGT 
GACACTGCAC CTTCATTTGA AGATTTAGTA GCAAAACACC TAGAAAAGTC CATCACAGTC 
AAACCAAGCA GCACATTCAG AGAAGCCTCA AGAAATACTT TGAGTCAGAA TGTATTTATG 
AATCTGAAGA TACTCTACGA CCTTACAATG GATCTTTGTT CTAAGTTGAA GGCTTATGGC 
TTATGGCAGC TATTTTGCAC TTTGGAGCTT CCATTGATAC CAATTTTGGC AGTGATGGAA 
AACCATAAGA TTCCAGTTGA CAAAGAAGAA ATGGAGAGGA CATCGGCACT CCTCGGGGCT 
CGCCTCAAGG AATTGGAGCA AGAAGCCCAT TTTGTTGCAG GAGAACAGTT TCTTATAATG 
AGTAATAATC AACTTCGTGA GATCCTCTTT GGCAAGTTGA AGCTGCACTT GTTGAGTCAG 
AGGAAACATC TTCCCAGAAC TGGGCTGCAG AACCAGCTGT CCACATCAGA AGCCATGTTA 
AATTCTCTAC AAGACCTTCA CCCATTACCC AAGTTAATTT TGGAATATAG ACAGGGCTCC 
ATCTCTTCTA CATGGAATCA GACTGGAACT GTGACAGGGA GACTTTCAGC CAAGCATCCA 
AATATCCAGG GTATCTCCAA GCACCCAATT AAGATTTCTA AGCCTTGGAA TTTTAAAGGT 
AAAGAAGAGG AGACTGTCAC CATCTCACCA AGAACCCTGT TTGTGTCCTC TGAAGGCCAC 
ACCTTCCTGG CAGCAGACTT TTCACAGATT GAATTGCGCA TTCTTGCACA TTTATCTGGG 
GATCCAGAGC TTTTGAAGTT ATTCCAGGAA TCTGAAAGAG ATGATGTATT TTCTACCCTG 
ACTTCACAGT GGAAGGACAT TCCCATAGAG CGTGTGACAC ACATGGACAG AGAACAAACC 
AAGAAAGTGG TGTACTCCGT GGTCTATGGA GCTGGGAAAG AGCGGCTTGC TGCTTGCCTT 
GGAGTCACTG TTTTGGAAGC CACCCACTTT TTGGAGAGAT TTTTGCAGAA GTACAAGAAA 
ATCAAGGACT TTGCCCAGAC AGTTATTGGC CAGTGTCACA GTGCAGGCTA TGTGACATCC 
ATCTTGGGTA GAAGGAGGCC TCTCCCGAGG ATCTGTGCAC AGGACCAGCA GCTTCGGGCA 
CAAGCAGAGC GACAGGCAGT AAACTTTGTG GTACAAGGCT CAGCCGCAGA CCTCTGTAAG 
CTAGCCATGA TTCGCATCTC TACTGCAGTA GCCACATCCC CGACCTTGAC AGCCAGGTTA 
GTTGCCCAAA TTCATGATGA GCTGCTGTTT GAAGTAGAAG ACACTCAGGT CCCTGAGTTT 
GCAGCTCTTG TCAGAAGGAT CATGGAGTCC TTGCAACAGG TACAGACCCT GGAACTGCAG 
CTGCAGGTGC CCCTAAAAGT GAACTTGAGT GTTGGCCGCT CATGGGGACA CCTGACCCCA 
CTGCAGGAGA TCCTGGGCTC AGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Poln ( NM_001289804.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001276733.1 Mus musculus DNA polymerase N (Poln), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu58328
Clone ID Related Accession (Same CDS sequence)	NM_001289804.1
Accession Version	NM_001289804.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Mus musculus(house mouse)
Product	DNA polymerase nu isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ083086.1, AK133063.1 and BY585631.1. Transcript Variant: This variant (3) differs in the 5' UTR and lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. It encodes isoform 3, which lacks an internal segment and is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK133063.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGAAAATGG AAAATTATGA GGCATGTGTA GGTTTTGATG TCTGTGAGAT ACCCCTCTCT 
GCTGTTGCTC AGAAGATTAT GTCTGCTATG CGTTCAGGTG ATTTCATGGA TTCTAGGAAT 
GAGGGAGAGA GTACAAACAC TTCAAAAGTG GCAAAGAAAT CCAGTGTTCA TTACTCAGTA 
TTAGCTGAAC ATGAGGAGAC TCAATCACTA GGAACAAAGA ACCCCGAGTC ATTAATAACC 
CAGACACCAA GAGGATCCAT TGAGCTCTGT CCTCAGCCCT CCATTACTAA GCTCACATGT 
CAGCTCTCTG CTGGTCAAGT ACAGAACAGC ATCAGCTCAT TGGGTCTTTC CAGTTATTTA 
ATTCCACAGT GTGATCAGGA AGCTTCAGTT TTACCGAACA TGGAGCATAA GAGACAGCAT 
TTCCTAAAGG AAAATATAGG CAAAGAAGAT AAGGACAACT CGAGTCTTAA AAGAAAATAT 
ATTACATGTA GTAAATCCTC AGAAAAAGCA AGTAAACACA CAGCTCTGGA AAAAGATACT 
GATGGGACTG AATCCTGGCC AAACTCCAGG GACACAAGAG CTTTGGGGGA AAGGCTGTGT 
GATGTTAGAT ATTTGGGTGA TTTGGCCAAA GCCCAGCTGA TGGATGCTCT CAAGCAGGCA 
GCAGCCCTGG TGGTGACATT GATGTACAAG GATGGTTCCA CACAGCTGAG CGCCAAGGAG 
GCTTTAACTT GTACTGTTAA AGGAATTGTA GTGTTACTAA AAAGCCATGT AGGCAACAGC 
ACTCTGACTC TCCCAGCCCA TGGTGGTGCT CTGGAAAAGG ACTTCATATC CGAAGATCAC 
TGTGTCTACA TACATACGGA GCATTCCCCT TTCTGGGACC CAAAGCAAGA AGCACATAGT 
CTATTTGTCA GGAACATACT GTTTTGGACA CTGAGGTGTA AATGTCCCGT TGTTTGTTTT 
AATGCCAAGG ACTTTGTGAG AACAGTGCTG CAGCTCTATG GTGAAGATGG CAGTTGGAAG 
CATGTTGCTG ATTTTGTAGG ACTAGATCCC AGAGTTGCTG CATGGCTCAT AGACCCCAGT 
GACACTGCAC CTTCATTTGA AGATTTAGTA GCAAAACACC TAGAAAAGTC CATCACAGTC 
AAACCAAGCA GCACATTCAG AGAAGCCTCA AGAAATACTT TGAGTCAGAA TGTATTTATG 
AATCTGAAGA TACTCTACGA CCTTACAATG GATCTTTGTT CTAAGTTGAA GGCTTATGGC 
TTATGGCAGC TATTTTGCAC TTTGGAGCTT CCATTGATAC CAATTTTGGC AGTGATGGAA 
AACCATAAGA TTCCAGTTGA CAAAGAAGAA ATGGAGAGGA CATCGGCACT CCTCGGGGCT 
CGCCTCAAGG AATTGGAGCA AGAAGCCCAT TTTGTTGCAG GAGAACAGTT TCTTATAATG 
AGTAATAATC AACTTCGTGA GATCCTCTTT GGCAAGTTGA AGCTGCACTT GTTGAGTCAG 
AGGAAACATC TTCCCAGAAC TGGGCTGCAG AACCAGCTGT CCACATCAGA AGCCATGTTA 
AATTCTCTAC AAGACCTTCA CCCATTACCC AAGTTAATTT TGGAATATAG ACAGGTTCAC 
AAGATCAAGT CAACTTTTAT AGATGGATTA CTAGCTTATA TGAAAAAGGG CTCCATCTCT 
TCTACATGGA ATCAGACTGG AACTGTGACA GGGAGACTTT CAGCCAAGCA TCCAAATATC 
CAGGGTATCT CCAAGCACCC AATTAAGATT TCTAAGCCTT GGAATTTTAA AGGTAAAGAA 
GAGGAGACTG TCACCATCTC ACCAAGAACC CTGTTTGTGT CCTCTGAAGG CCACACCTTC 
CTGGCAGCAG ACTTTTCACA GATTGAATTG CGCATTCTTG CACATTTATC TGGGGATCCA 
GAGCTTTTGA AGTTATTCCA GGAATCTGAA AGAGATGATG TATTTTCTAC CCTGACTTCA 
CAGTGGAAGG ACATTCCCAT AGAGCGTGTG ACACACATGG ACAGAGAACA AACCAAGAAA 
GTGGTGTACT CCGTGGTCTA TGGAGCTGGC TATGTGACAT CCATCTTGGG TAGAAGGAGG 
CCTCTCCCGA GGATCTGTGC ACAGGACCAG CAGCTTCGGG CACAAGCAGA GCGACAGGCA 
GTAAACTTTG TGGTACAAGG CTCAGCCGCA GACCTCTGTA AGCTAGCCAT GATTCGCATC 
TCTACTGCAG TAGCCACATC CCCGACCTTG ACAGCCAGGT TAGTTGCCCA AATTCATGAT 
GAGCTGCTGT TTGAAGTAGA AGACACTCAG GTCCCTGAGT TTGCAGCTCT TGTCAGAAGG 
ATCATGGAGT CCTTGCAACA GGTACAGACC CTGGAACTGC AGCTGCAGGT GCCCCTAAAA 
GTGAACTTGA GTGTTGGCCG CTCATGGGGA CACCTGACCC CACTGCAGGA GATCCTGGGC 
TCAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276733.1
CDS	193..2661
Translation	MKMENYEACVGFDVCEIPLSAVAQKIMSAMRSGDFMDSRNEGESTNTSKVAKKSSVHYSVLAEHEETQSLGTKNPESLITQTPRGSIELCPQPSITKLTCQLSAGQVQNSISSLGLSSYLIPQCDQEASVLPNMEHKRQHFLKENIGKEDKDNSSLKRKYITCSKSSEKASKHTALEKDTDGTESWPNSRDTRALGERLCDVRYLGDLAKAQLMDALKQAAALVVTLMYKDGSTQLSAKEALTCTVKGIVVLLKSHVGNSTLTLPAHGGALEKDFISEDHCVYIHTEHSPFWDPKQEAHSLFVRNILFWTLRCKCPVVCFNAKDFVRTVLQLYGEDGSWKHVADFVGLDPRVAAWLIDPSDTAPSFEDLVAKHLEKSITVKPSSTFREASRNTLSQNVFMNLKILYDLTMDLCSKLKAYGLWQLFCTLELPLIPILAVMENHKIPVDKEEMERTSALLGARLKELEQEAHFVAGEQFLIMSNNQLREILFGKLKLHLLSQRKHLPRTGLQNQLSTSEAMLNSLQDLHPLPKLILEYRQVHKIKSTFIDGLLAYMKKGSISSTWNQTGTVTGRLSAKHPNIQGISKHPIKISKPWNFKGKEEETVTISPRTLFVSSEGHTFLAADFSQIELRILAHLSGDPELLKLFQESERDDVFSTLTSQWKDIPIERVTHMDREQTKKVVYSVVYGAGYVTSILGRRRPLPRICAQDQQLRAQAERQAVNFVVQGSAADLCKLAMIRISTAVATSPTLTARLVAQIHDELLFEVEDTQVPEFAALVRRIMESLQQVQTLELQLQVPLKVNLSVGRSWGHLTPLQEILGSA

Target ORF information:

RefSeq Version	NM_001289804.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DNA polymerase N (Poln), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289804.1

ORF Insert Sequence:

ATGAAAATGG AAAATTATGA GGCATGTGTA GGTTTTGATG TCTGTGAGAT ACCCCTCTCT 
GCTGTTGCTC AGAAGATTAT GTCTGCTATG CGTTCAGGTG ATTTCATGGA TTCTAGGAAT 
GAGGGAGAGA GTACAAACAC TTCAAAAGTG GCAAAGAAAT CCAGTGTTCA TTACTCAGTA 
TTAGCTGAAC ATGAGGAGAC TCAATCACTA GGAACAAAGA ACCCCGAGTC ATTAATAACC 
CAGACACCAA GAGGATCCAT TGAGCTCTGT CCTCAGCCCT CCATTACTAA GCTCACATGT 
CAGCTCTCTG CTGGTCAAGT ACAGAACAGC ATCAGCTCAT TGGGTCTTTC CAGTTATTTA 
ATTCCACAGT GTGATCAGGA AGCTTCAGTT TTACCGAACA TGGAGCATAA GAGACAGCAT 
TTCCTAAAGG AAAATATAGG CAAAGAAGAT AAGGACAACT CGAGTCTTAA AAGAAAATAT 
ATTACATGTA GTAAATCCTC AGAAAAAGCA AGTAAACACA CAGCTCTGGA AAAAGATACT 
GATGGGACTG AATCCTGGCC AAACTCCAGG GACACAAGAG CTTTGGGGGA AAGGCTGTGT 
GATGTTAGAT ATTTGGGTGA TTTGGCCAAA GCCCAGCTGA TGGATGCTCT CAAGCAGGCA 
GCAGCCCTGG TGGTGACATT GATGTACAAG GATGGTTCCA CACAGCTGAG CGCCAAGGAG 
GCTTTAACTT GTACTGTTAA AGGAATTGTA GTGTTACTAA AAAGCCATGT AGGCAACAGC 
ACTCTGACTC TCCCAGCCCA TGGTGGTGCT CTGGAAAAGG ACTTCATATC CGAAGATCAC 
TGTGTCTACA TACATACGGA GCATTCCCCT TTCTGGGACC CAAAGCAAGA AGCACATAGT 
CTATTTGTCA GGAACATACT GTTTTGGACA CTGAGGTGTA AATGTCCCGT TGTTTGTTTT 
AATGCCAAGG ACTTTGTGAG AACAGTGCTG CAGCTCTATG GTGAAGATGG CAGTTGGAAG 
CATGTTGCTG ATTTTGTAGG ACTAGATCCC AGAGTTGCTG CATGGCTCAT AGACCCCAGT 
GACACTGCAC CTTCATTTGA AGATTTAGTA GCAAAACACC TAGAAAAGTC CATCACAGTC 
AAACCAAGCA GCACATTCAG AGAAGCCTCA AGAAATACTT TGAGTCAGAA TGTATTTATG 
AATCTGAAGA TACTCTACGA CCTTACAATG GATCTTTGTT CTAAGTTGAA GGCTTATGGC 
TTATGGCAGC TATTTTGCAC TTTGGAGCTT CCATTGATAC CAATTTTGGC AGTGATGGAA 
AACCATAAGA TTCCAGTTGA CAAAGAAGAA ATGGAGAGGA CATCGGCACT CCTCGGGGCT 
CGCCTCAAGG AATTGGAGCA AGAAGCCCAT TTTGTTGCAG GAGAACAGTT TCTTATAATG 
AGTAATAATC AACTTCGTGA GATCCTCTTT GGCAAGTTGA AGCTGCACTT GTTGAGTCAG 
AGGAAACATC TTCCCAGAAC TGGGCTGCAG AACCAGCTGT CCACATCAGA AGCCATGTTA 
AATTCTCTAC AAGACCTTCA CCCATTACCC AAGTTAATTT TGGAATATAG ACAGGTTCAC 
AAGATCAAGT CAACTTTTAT AGATGGATTA CTAGCTTATA TGAAAAAGGG CTCCATCTCT 
TCTACATGGA ATCAGACTGG AACTGTGACA GGGAGACTTT CAGCCAAGCA TCCAAATATC 
CAGGGTATCT CCAAGCACCC AATTAAGATT TCTAAGCCTT GGAATTTTAA AGGTAAAGAA 
GAGGAGACTG TCACCATCTC ACCAAGAACC CTGTTTGTGT CCTCTGAAGG CCACACCTTC 
CTGGCAGCAG ACTTTTCACA GATTGAATTG CGCATTCTTG CACATTTATC TGGGGATCCA 
GAGCTTTTGA AGTTATTCCA GGAATCTGAA AGAGATGATG TATTTTCTAC CCTGACTTCA 
CAGTGGAAGG ACATTCCCAT AGAGCGTGTG ACACACATGG ACAGAGAACA AACCAAGAAA 
GTGGTGTACT CCGTGGTCTA TGGAGCTGGC TATGTGACAT CCATCTTGGG TAGAAGGAGG 
CCTCTCCCGA GGATCTGTGC ACAGGACCAG CAGCTTCGGG CACAAGCAGA GCGACAGGCA 
GTAAACTTTG TGGTACAAGG CTCAGCCGCA GACCTCTGTA AGCTAGCCAT GATTCGCATC 
TCTACTGCAG TAGCCACATC CCCGACCTTG ACAGCCAGGT TAGTTGCCCA AATTCATGAT 
GAGCTGCTGT TTGAAGTAGA AGACACTCAG GTCCCTGAGT TTGCAGCTCT TGTCAGAAGG 
ATCATGGAGT CCTTGCAACA GGTACAGACC CTGGAACTGC AGCTGCAGGT GCCCCTAAAA 
GTGAACTTGA GTGTTGGCCG CTCATGGGGA CACCTGACCC CACTGCAGGA GATCCTGGGC 
TCAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Poln ( NM_181857.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_862905.1 Mus musculus DNA polymerase N (Poln), transcript variant 1, mRNA.

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CloneID	OMu58329
Clone ID Related Accession (Same CDS sequence)	NM_181857.4
Accession Version	NM_181857.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Mus musculus(house mouse)
Product	DNA polymerase nu isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ083086.1, AK133063.1, AC107709.12 and BY585631.1. On Jan 31, 2014 this sequence version replaced NM_181857.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: AY135562.3, BC141101.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGAAAATGG AAAATTATGA GGCATGTGTA GGTTTTGATG TCTGTGAGAT ACCCCTCTCT 
GCTGTTGCTC AGAAGATTAT GTCTGCTATG CGTTCAGGTG ATTTCATGGA TTCTAGGAAT 
GAGGGAGAGA GTACAAACAC TTCAAAAGTG GCAAAGAAAT CCAGTGTTCA TTACTCAGTA 
TTAGCTGAAC ATGAGGAGAC TCAATCACTA GGAACAAAGA ACCCCGAGTC ATTAATAACC 
CAGACACCAA GAGGATCCAT TGAGCTCTGT CCTCAGCCCT CCATTACTAA GCTCACATGT 
CAGCTCTCTG CTGGTCAAGT ACAGAACAGC ATCAGCTCAT TGGGTCTTTC CAGTTATTTA 
ATTCCACAGT GTGATCAGGA AGCTTCAGTT TTACCGAACA TGGAGCATAA GAGACAGCAT 
TTCCTAAAGG AAAATATAGG CAAAGAAGAT AAGGACAACT CGAGTCTTAA AAGAAAATAT 
ATTACATGTA GTAAATCCTC AGAAAAAGCA AGTAAACACA CAGCTCTGGA AAAAGATACT 
GATGGGACTG AATCCTGGCC AAACTCCAGG GACACAAGAG CTTTGGGGGA AAGGCTGTGT 
GATGTTAGAT ATTTGGGTGA TTTGGCCAAA GCCCAGCTGA TGGATGCTCT CAAGCAGGCA 
GCAGCCCTGG TGGTGACATT GATGTACAAG GATGGTTCCA CACAGCTGAG CGCCAAGGAG 
GCTTTAACTT GTACTGTTAA AGGAATTGTA GTGTTACTAA AAAGCCATGT AGGCAACAGC 
ACTCTGACTC TCCCAGCCCA TGGTGGTGCT CTGGAAAAGG ACTTCATATC CGAAGATCAC 
TGTGTCTACA TACATACGGA GCATTCCCCT TTCTGGGACC CAAAGCAAGA AGCACATAGT 
CTATTTGTCA GGAACATACT GTTTTGGACA CTGAGGTGTA AATGTCCCGT TGTTTGTTTT 
AATGCCAAGG ACTTTGTGAG AACAGTGCTG CAGCTCTATG GTGAAGATGG CAGTTGGAAG 
CATGTTGCTG ATTTTGTAGG ACTAGATCCC AGAGTTGCTG CATGGCTCAT AGACCCCAGT 
GACACTGCAC CTTCATTTGA AGATTTAGTA GCAAAACACC TAGAAAAGTC CATCACAGTC 
AAACCAAGCA GCACATTCAG AGAAGCCTCA AGAAATACTT TGAGTCAGAA TGTATTTATG 
AATCTGAAGA TACTCTACGA CCTTACAATG GATCTTTGTT CTAAGTTGAA GGCTTATGGC 
TTATGGCAGC TATTTTGCAC TTTGGAGCTT CCATTGATAC CAATTTTGGC AGTGATGGAA 
AACCATAAGA TTCCAGTTGA CAAAGAAGAA ATGGAGAGGA CATCGGCACT CCTCGGGGCT 
CGCCTCAAGG AATTGGAGCA AGAAGCCCAT TTTGTTGCAG GAGAACAGTT TCTTATAATG 
AGTAATAATC AACTTCGTGA GATCCTCTTT GGCAAGTTGA AGCTGCACTT GTTGAGTCAG 
AGGAAACATC TTCCCAGAAC TGGGCTGCAG AACCAGCTGT CCACATCAGA AGCCATGTTA 
AATTCTCTAC AAGACCTTCA CCCATTACCC AAGTTAATTT TGGAATATAG ACAGGTTCAC 
AAGATCAAGT CAACTTTTAT AGATGGATTA CTAGCTTATA TGAAAAAGGG CTCCATCTCT 
TCTACATGGA ATCAGACTGG AACTGTGACA GGGAGACTTT CAGCCAAGCA TCCAAATATC 
CAGGGTATCT CCAAGCACCC AATTAAGATT TCTAAGCCTT GGAATTTTAA AGGTAAAGAA 
GAGGAGACTG TCACCATCTC ACCAAGAACC CTGTTTGTGT CCTCTGAAGG CCACACCTTC 
CTGGCAGCAG ACTTTTCACA GATTGAATTG CGCATTCTTG CACATTTATC TGGGGATCCA 
GAGCTTTTGA AGTTATTCCA GGAATCTGAA AGAGATGATG TATTTTCTAC CCTGACTTCA 
CAGTGGAAGG ACATTCCCAT AGAGCGTGTG ACACACATGG ACAGAGAACA AACCAAGAAA 
GTGGTGTACT CCGTGGTCTA TGGAGCTGGG AAAGAGCGGC TTGCTGCTTG CCTTGGAGTC 
ACTGTTTTGG AAGCCACCCA CTTTTTGGAG AGATTTTTGC AGAAGTACAA GAAAATCAAG 
GACTTTGCCC AGACAGTTAT TGGCCAGTGT CACAGTGCAG GCTATGTGAC ATCCATCTTG 
GGTAGAAGGA GGCCTCTCCC GAGGATCTGT GCACAGGACC AGCAGCTTCG GGCACAAGCA 
GAGCGACAGG CAGTAAACTT TGTGGTACAA GGCTCAGCCG CAGACCTCTG TAAGCTAGCC 
ATGATTCGCA TCTCTACTGC AGTAGCCACA TCCCCGACCT TGACAGCCAG GTTAGTTGCC 
CAAATTCATG ATGAGCTGCT GTTTGAAGTA GAAGACACTC AGGTCCCTGA GTTTGCAGCT 
CTTGTCAGAA GGATCATGGA GTCCTTGCAA CAGGTACAGA CCCTGGAACT GCAGCTGCAG 
GTGCCCCTAA AAGTGAACTT GAGTGTTGGC CGCTCATGGG GACACCTGAC CCCACTGCAG 
GAGATCCTGG GCTCAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_862905.1
CDS	144..2744
Translation	MKMENYEACVGFDVCEIPLSAVAQKIMSAMRSGDFMDSRNEGESTNTSKVAKKSSVHYSVLAEHEETQSLGTKNPESLITQTPRGSIELCPQPSITKLTCQLSAGQVQNSISSLGLSSYLIPQCDQEASVLPNMEHKRQHFLKENIGKEDKDNSSLKRKYITCSKSSEKASKHTALEKDTDGTESWPNSRDTRALGERLCDVRYLGDLAKAQLMDALKQAAALVVTLMYKDGSTQLSAKEALTCTVKGIVVLLKSHVGNSTLTLPAHGGALEKDFISEDHCVYIHTEHSPFWDPKQEAHSLFVRNILFWTLRCKCPVVCFNAKDFVRTVLQLYGEDGSWKHVADFVGLDPRVAAWLIDPSDTAPSFEDLVAKHLEKSITVKPSSTFREASRNTLSQNVFMNLKILYDLTMDLCSKLKAYGLWQLFCTLELPLIPILAVMENHKIPVDKEEMERTSALLGARLKELEQEAHFVAGEQFLIMSNNQLREILFGKLKLHLLSQRKHLPRTGLQNQLSTSEAMLNSLQDLHPLPKLILEYRQVHKIKSTFIDGLLAYMKKGSISSTWNQTGTVTGRLSAKHPNIQGISKHPIKISKPWNFKGKEEETVTISPRTLFVSSEGHTFLAADFSQIELRILAHLSGDPELLKLFQESERDDVFSTLTSQWKDIPIERVTHMDREQTKKVVYSVVYGAGKERLAACLGVTVLEATHFLERFLQKYKKIKDFAQTVIGQCHSAGYVTSILGRRRPLPRICAQDQQLRAQAERQAVNFVVQGSAADLCKLAMIRISTAVATSPTLTARLVAQIHDELLFEVEDTQVPEFAALVRRIMESLQQVQTLELQLQVPLKVNLSVGRSWGHLTPLQEILGSA

Target ORF information:

RefSeq Version	NM_181857.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus DNA polymerase N (Poln), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181857.4

ORF Insert Sequence:

ATGAAAATGG AAAATTATGA GGCATGTGTA GGTTTTGATG TCTGTGAGAT ACCCCTCTCT 
GCTGTTGCTC AGAAGATTAT GTCTGCTATG CGTTCAGGTG ATTTCATGGA TTCTAGGAAT 
GAGGGAGAGA GTACAAACAC TTCAAAAGTG GCAAAGAAAT CCAGTGTTCA TTACTCAGTA 
TTAGCTGAAC ATGAGGAGAC TCAATCACTA GGAACAAAGA ACCCCGAGTC ATTAATAACC 
CAGACACCAA GAGGATCCAT TGAGCTCTGT CCTCAGCCCT CCATTACTAA GCTCACATGT 
CAGCTCTCTG CTGGTCAAGT ACAGAACAGC ATCAGCTCAT TGGGTCTTTC CAGTTATTTA 
ATTCCACAGT GTGATCAGGA AGCTTCAGTT TTACCGAACA TGGAGCATAA GAGACAGCAT 
TTCCTAAAGG AAAATATAGG CAAAGAAGAT AAGGACAACT CGAGTCTTAA AAGAAAATAT 
ATTACATGTA GTAAATCCTC AGAAAAAGCA AGTAAACACA CAGCTCTGGA AAAAGATACT 
GATGGGACTG AATCCTGGCC AAACTCCAGG GACACAAGAG CTTTGGGGGA AAGGCTGTGT 
GATGTTAGAT ATTTGGGTGA TTTGGCCAAA GCCCAGCTGA TGGATGCTCT CAAGCAGGCA 
GCAGCCCTGG TGGTGACATT GATGTACAAG GATGGTTCCA CACAGCTGAG CGCCAAGGAG 
GCTTTAACTT GTACTGTTAA AGGAATTGTA GTGTTACTAA AAAGCCATGT AGGCAACAGC 
ACTCTGACTC TCCCAGCCCA TGGTGGTGCT CTGGAAAAGG ACTTCATATC CGAAGATCAC 
TGTGTCTACA TACATACGGA GCATTCCCCT TTCTGGGACC CAAAGCAAGA AGCACATAGT 
CTATTTGTCA GGAACATACT GTTTTGGACA CTGAGGTGTA AATGTCCCGT TGTTTGTTTT 
AATGCCAAGG ACTTTGTGAG AACAGTGCTG CAGCTCTATG GTGAAGATGG CAGTTGGAAG 
CATGTTGCTG ATTTTGTAGG ACTAGATCCC AGAGTTGCTG CATGGCTCAT AGACCCCAGT 
GACACTGCAC CTTCATTTGA AGATTTAGTA GCAAAACACC TAGAAAAGTC CATCACAGTC 
AAACCAAGCA GCACATTCAG AGAAGCCTCA AGAAATACTT TGAGTCAGAA TGTATTTATG 
AATCTGAAGA TACTCTACGA CCTTACAATG GATCTTTGTT CTAAGTTGAA GGCTTATGGC 
TTATGGCAGC TATTTTGCAC TTTGGAGCTT CCATTGATAC CAATTTTGGC AGTGATGGAA 
AACCATAAGA TTCCAGTTGA CAAAGAAGAA ATGGAGAGGA CATCGGCACT CCTCGGGGCT 
CGCCTCAAGG AATTGGAGCA AGAAGCCCAT TTTGTTGCAG GAGAACAGTT TCTTATAATG 
AGTAATAATC AACTTCGTGA GATCCTCTTT GGCAAGTTGA AGCTGCACTT GTTGAGTCAG 
AGGAAACATC TTCCCAGAAC TGGGCTGCAG AACCAGCTGT CCACATCAGA AGCCATGTTA 
AATTCTCTAC AAGACCTTCA CCCATTACCC AAGTTAATTT TGGAATATAG ACAGGTTCAC 
AAGATCAAGT CAACTTTTAT AGATGGATTA CTAGCTTATA TGAAAAAGGG CTCCATCTCT 
TCTACATGGA ATCAGACTGG AACTGTGACA GGGAGACTTT CAGCCAAGCA TCCAAATATC 
CAGGGTATCT CCAAGCACCC AATTAAGATT TCTAAGCCTT GGAATTTTAA AGGTAAAGAA 
GAGGAGACTG TCACCATCTC ACCAAGAACC CTGTTTGTGT CCTCTGAAGG CCACACCTTC 
CTGGCAGCAG ACTTTTCACA GATTGAATTG CGCATTCTTG CACATTTATC TGGGGATCCA 
GAGCTTTTGA AGTTATTCCA GGAATCTGAA AGAGATGATG TATTTTCTAC CCTGACTTCA 
CAGTGGAAGG ACATTCCCAT AGAGCGTGTG ACACACATGG ACAGAGAACA AACCAAGAAA 
GTGGTGTACT CCGTGGTCTA TGGAGCTGGG AAAGAGCGGC TTGCTGCTTG CCTTGGAGTC 
ACTGTTTTGG AAGCCACCCA CTTTTTGGAG AGATTTTTGC AGAAGTACAA GAAAATCAAG 
GACTTTGCCC AGACAGTTAT TGGCCAGTGT CACAGTGCAG GCTATGTGAC ATCCATCTTG 
GGTAGAAGGA GGCCTCTCCC GAGGATCTGT GCACAGGACC AGCAGCTTCG GGCACAAGCA 
GAGCGACAGG CAGTAAACTT TGTGGTACAA GGCTCAGCCG CAGACCTCTG TAAGCTAGCC 
ATGATTCGCA TCTCTACTGC AGTAGCCACA TCCCCGACCT TGACAGCCAG GTTAGTTGCC 
CAAATTCATG ATGAGCTGCT GTTTGAAGTA GAAGACACTC AGGTCCCTGA GTTTGCAGCT 
CTTGTCAGAA GGATCATGGA GTCCTTGCAA CAGGTACAGA CCCTGGAACT GCAGCTGCAG 
GTGCCCCTAA AAGTGAACTT GAGTGTTGGC CGCTCATGGG GACACCTGAC CCCACTGCAG 
GAGATCCTGG GCTCAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Analysis of DNA polymerase ? function in meiotic recombination, immunoglobulin class-switching, and DNA damage tolerance.
PLoS genetics13(6)e1006818(2017 Jun)
Takata KI,Reh S,Yousefzadeh MJ,Zelazowski MJ,Bhetawal S,Trono D,Lowery MG,Sandoval M,Takata Y,Lu Y,Lin K,Shen J,Kusewitt DF,McBride KM,Cole F,Wood RD

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Ubiquitin ligase Rad18Sc localizes to the XY body and to other chromosomal regions that are unpaired and transcriptionally silenced during male meiotic prophase.
Journal of cell science117(Pt 21)5023-33(2004 Oct)
van der Laan R,Uringa EJ,Wassenaar E,Hoogerbrugge JW,Sleddens E,Odijk H,Roest HP,de Boer P,Hoeijmakers JH,Grootegoed JA,Baarends WM

POLN, a nuclear PolA family DNA polymerase homologous to the DNA cross-link sensitivity protein Mus308.
The Journal of biological chemistry278(34)32014-9(2003 Aug)
Marini F,Kim N,Schuffert A,Wood RD

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Poln cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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