Npff cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Npff
Entrez Gene ID	54615
Full Name	neuropeptide FF-amide peptide precursor
General protein information	Preferred Names neuropeptide FF-amide peptide precursor Names pro-FMRFamide-related neuropeptide FF FMRFamide-related peptides
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 F3
Summary	This gene encodes neuropeptides involved in analgesia, cardiovascular regulation, neuroendocrine function and opiate addiction. The encoded protein is a preproprotein that undergoes further processing to generate multiple amidated peptides. [provided by RefSeq, Jul 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_018787.1	NP_061257.1	pro-FMRFamide-related neuropeptide FF preproprotein

Pathways from BioSystems

Homologies

	NPFF	XP_001106733.1
Mus musculus (house mouse)	Npff	NP_061257.1
Pan troglodytes (chimpanzee)	NPFF	XP_001138078.1
Rattus norvegicus (Norway rat)	Npff	NP_072108.1
Homo sapiens (human)	NPFF	NP_003708.1
Bos taurus (cattle)	NPFF	NP_776548.1

Gene Ontology
Bibliography

Related articles in PubMed

NPFFR2 Activates the HPA Axis and Induces Anxiogenic Effects in Rodents.
Lin YT, Yu YL, Hong WC, Yeh TS, Chen TC, Chen JC
International journal of molecular sciences18(8)(2017 Aug)

Neuropeptide FF increases M2 activation and self-renewal of adipose tissue macrophages.
Waqas SFH, Hoang AC, Lin YT, Ampem G, Azegrouz H, Balogh L, Thur?czy J, Chen JC, Gerling IC, Nam S, Lim JS, Martinez-Iba?ez J, Real JT, Paschke S, Quillet R, Ayachi S, Simonin F, Schneider EM, Brinkman JA, Lamming DW, Seroogy CM, R?szer T
The Journal of clinical investigation127(7)2842-2854(2017 Jun)

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

NPFF2 receptor is involved in the modulatory effects of neuropeptide FF for macrophage cell line.
Sun YL, Sun T, Zhang XY, He N, Zhuang Y, Li JY, Fang Q, Wang KR, Wang R
Protein and peptide letters21(5)490-502(2014 May)

Central locomotor and cognitive effects of a NPFF receptor agonist in mouse.
Betourne A, Marty V, Ceccom J, Halley H, Lassalle JM, Zajac JM, Frances B, Mouledous L
Peptides31(2)221-6(2010 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NPFF thus plays an important role in supporting healthy adipose tissue via the maintenance of metabolically beneficial adipose tissue macrophages.
Title: Neuropeptide FF increases M2 activation and self-renewal of adipose tissue macrophages.

Down-regulation of nitric oxide (NO) synthases (NOSs) partially significantly inhibited the viability augmentation of macrophages induced by Npff, implying a nitric oxide synthases- dependent pathway is involved.
Title: NPFF2 receptor is involved in the modulatory effects of neuropeptide FF for macrophage cell line.

results demonstrate a small but complex influence of the NPFF system on mouse behavior
Title: Central locomotor and cognitive effects of a NPFF receptor agonist in mouse.

Npff reduces the expression of cocaine-induced conditioned place preference and cocaine-induce sensitization in mice.
Title: Neuropeptide FF (NPFF) reduces the expression of cocaine-induced conditioned place preference and cocaine-induced sensitization in animals.

We postulate that Oct-1, PDX1, Pit-1, MEIS and DREAM are likely transcriptional regulators of NPFF gene expression.
Title: Identification of transcriptional regulators of neuropeptide FF gene expression.

The following Npff gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Npff cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu11005	NM_018787.1 Latest version!	Mus musculus neuropeptide FF-amide peptide precursor (Npff), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Npff ( NM_018787.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_061257.1 Mus musculus neuropeptide FF-amide peptide precursor (Npff), mRNA.

Price & Availability↑

CloneID	OMu11005
Clone ID Related Accession (Same CDS sequence)	NM_018787.1
Accession Version	NM_018787.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	pro-FMRFamide-related neuropeptide FF preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF148701.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF148701.1, SRR1660815.21063.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGACTCCA AGTGGGCTGC TCTGCTGCTG CTGCTACTGC TGCTGCTGAA TTGGGGCCAC 
ACTGAAGAGG CAGGGAGCTG GGGTGAAGAC CAAGTCTTTG CAGGAGAAGA TAAGGGACCC 
CACCCACCAC AGTATGCCCA CATTCCAGAC AGGATCCAGA CTCCTGGGTC CCTCTTTCGT 
GTTCTGCTCC AGGCCATGGA CACACCTAGA AGGAGCCCAG CCTTCCTGTT TCAGCCCCAG 
AGGTTTGGCA GAAGTGCATG GGGGTCCTGG AGCAAGGAAC AGCTAAATCC GCAGGCCAGA 
CAGTTCTGGA GCCTGGCCGC TCCTCAGCGC TTTGGGAAGA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061257.1
CDS	14..358
Translation	MDSKWAALLLLLLLLLNWGHTEEAGSWGEDQVFAGEDKGPHPPQYAHIPDRIQTPGSLFRVLLQAMDTPRRSPAFLFQPQRFGRSAWGSWSKEQLNPQARQFWSLAAPQRFGKK

Target ORF information:

RefSeq Version	NM_018787.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus neuropeptide FF-amide peptide precursor (Npff), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018787.1

ORF Insert Sequence:

ATGGACTCCA AGTGGGCTGC TCTGCTGCTG CTGCTACTGC TGCTGCTGAA TTGGGGCCAC 
ACTGAAGAGG CAGGGAGCTG GGGTGAAGAC CAAGTCTTTG CAGGAGAAGA TAAGGGACCC 
CACCCACCAC AGTATGCCCA CATTCCAGAC AGGATCCAGA CTCCTGGGTC CCTCTTTCGT 
GTTCTGCTCC AGGCCATGGA CACACCTAGA AGGAGCCCAG CCTTCCTGTT TCAGCCCCAG 
AGGTTTGGCA GAAGTGCATG GGGGTCCTGG AGCAAGGAAC AGCTAAATCC GCAGGCCAGA 
CAGTTCTGGA GCCTGGCCGC TCCTCAGCGC TTTGGGAAGA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NPFFR2 Activates the HPA Axis and Induces Anxiogenic Effects in Rodents.
International journal of molecular sciences18(8)(2017 Aug)
Lin YT,Yu YL,Hong WC,Yeh TS,Chen TC,Chen JC

Neuropeptide FF increases M2 activation and self-renewal of adipose tissue macrophages.
The Journal of clinical investigation127(7)2842-2854(2017 Jun)
Waqas SFH,Hoang AC,Lin YT,Ampem G,Azegrouz H,Balogh L,Thur?czy J,Chen JC,Gerling IC,Nam S,Lim JS,Martinez-Iba?ez J,Real JT,Paschke S,Quillet R,Ayachi S,Simonin F,Schneider EM,Brinkman JA,Lamming DW,Seroogy CM,R?szer T

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

NPFF2 receptor is involved in the modulatory effects of neuropeptide FF for macrophage cell line.
Protein and peptide letters21(5)490-502(2014 May)
Sun YL,Sun T,Zhang XY,He N,Zhuang Y,Li JY,Fang Q,Wang KR,Wang R

Central locomotor and cognitive effects of a NPFF receptor agonist in mouse.
Peptides31(2)221-6(2010 Feb)
Betourne A,Marty V,Ceccom J,Halley H,Lassalle JM,Zajac JM,Frances B,Mouledous L

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Npff cDNA ORF clone, Mus musculus(house mouse)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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