Nhej1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Nhej1
Entrez Gene ID	75570
Full Name	nonhomologous end-joining factor 1
Synonyms	1700029B21Rik,XLF,cernunnos
General protein information	Preferred Names nonhomologous end-joining factor 1 Names non-homologous end-joining factor 1 XRCC4-like factor protein cernunnos
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 C4

mRNA and Protein(s)

mRNA Protein Name

NM_029342.4 NP_083618.3 non-homologous end-joining factor 1
Pathways from BioSystems

mRNA	Protein	Name
NM_029342.4	NP_083618.3	non-homologous end-joining factor 1

Homologies

Homo sapiens (human)	NHEJ1	NP_079058.1
Mus musculus (house mouse)	Nhej1	NP_083618.3
Danio rerio (zebrafish)	nhej1	NP_999914.1
Gallus gallus (chicken)	NHEJ1	XP_422052.2
Xenopus tropicalis (tropical clawed frog)	nhej1	XP_002933989.2
Bos taurus (cattle)	NHEJ1	NP_001068861.1
Canis lupus familiaris (dog)	NHEJ1	XP_853192.2
Rattus norvegicus (Norway rat)	Nhej1	NP_001014239.1
Pan troglodytes (chimpanzee)	NHEJ1	XP_001160321.1

Gene Ontology
Bibliography

Related articles in PubMed

PAXX and Xlf interplay revealed by impaired CNS development and immunodeficiency of double KO mice.
Abramowski V, Etienne O, Elsaid R, Yang J, Berland A, Kermasson L, Roch B, Musilli S, Moussu JP, Lipson-Ruffert K, Revy P, Cumano A, Boussin FD, de Villartay JP
Cell death and differentiation25(2)444-452(2018 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ku70 is epistatic with XLF and DNA-PKcs and support a model in which inactivation of Ku70 allows DNA lesions to become accessible to alternative DNA repair pathways (other than Classical Non-Homologous End-Joining (C-NHEJ)).
Title: Synthetic lethality between murine DNA repair factors XLF and DNA-PKcs is rescued by inactivation of Ku70.

PAXX and XLF proteins may have redundant functions during Non-homologous end joining.
Title: Deficiency of XLF and PAXX prevents DNA double-strand break repair by non-homologous end joining in lymphocytes.

Paxx/Xlf double-knockout mice display embryonic lethality associated with genomic instability, cell death in the central nervous system, and an almost complete block in lymphogenesis
Title: Synthetic lethality between PAXX and XLF in mammalian development.

XLF has an important role during V(D)J recombination.
Title: XLF deficiency results in reduced N-nucleotide addition during V(D)J recombination.

These results reveal an unanticipated functional interplay between the RAG complex and XLF in repairing RAG-induced DNA breaks and maintaining genome integrity during antigen receptor gene assembly.
Title: RAG2 and XLF/Cernunnos interplay reveals a novel role for the RAG complex in DNA repair.

The following Nhej1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nhej1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu06838	NM_029342.4 Latest version!	Mus musculus non-homologous end joining factor 1 (Nhej1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nhej1 ( NM_029342.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083618.3 Mus musculus non-homologous end joining factor 1 (Nhej1), mRNA.

Price & Availability↑

CloneID	OMu06838
Clone ID Related Accession (Same CDS sequence)	NM_029342.4
Accession Version	NM_029342.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	non-homologous end-joining factor 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY095373.1, BC107252.1, BY432477.1 and AV512639.1. On Oct 20, 2007 this sequence version replaced NM_029342.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN01164142, SAMN01164143 [ECO:0000350] ##Evidence-Data-END##

ATGGAAGAGC TAGAGCAAGA CCTGTTGCTG CAGCCATGGG CATGGTTACA ACTTGCGGAG 
AACTCACTCT TAGCCAAGGT GTCTATCACC AAGCACGGTT ATGCCTTGCT GATTTCGGAT 
CTTCAACAGG TGTGGCATGA ACAGGTGGAC ACTTCGGTGG TCAGCCAGCG AGCCAAGGAG 
CTGAACAAGC GCCTCACTGC GCCTCCTGCA GCTTTGCTCT GTCACCTGGA TGAAGCACTT 
CGCCCACTGT TTAAAGATTC TGCTCACCCT AGCAAAGCTA CTTTCTCCTG TGACCGAGGA 
GAGGAGGGAC TGATCCTGCG GGTGCAGAGT GAGCTCTCGG GTCTTCCCTT CAGTTGGCAT 
TTCCACTGTA TTCCAGCTAG TTCTTCACTG GTCTCTCAGC ATTTGATTCA TCCTCTGATG 
GGTGTGAGCC TGGCACTGCA GAGTCATGTG AGGGAGCTAG CAGCATTGCT TCGGATGAAG 
GACCTTGAGA TCCAGGCCTA CCAGGAGAGT GGGGCTGTGC TGAGCCGAAG TCGATTGAAG 
ACAGAGCCAT TTGAAGAAAA TTCTTTCTTG GAACAGTTTA TGGCAGAGAA ATTGCCAGAG 
GCGTGTGCTG TTGGTGATGG AAAGCCATTT GCCATGAGTC TGCAGAGTCT GTATGTGGCA 
GTTACAAAAC AGCAGATCCA AGCAAGGCAG GCACATAAAG ACTCTGGAGA GACTCAGGCA 
TCAAGCAGCA CCTCCCCTCG AGGAACTGAT AACCAGCCAG AAGAGCCGGT CTCCCTCTCT 
TCCACCCTCT CAGAGCCTGA ATATGAGCCT GTGGCTGCTT CAGGCCCTAT GCATAGAGCT 
CGGCTGGTGA AGTCCAAGAG GAAGAAGCCC AGGGGACTCT TCAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083618.3
CDS	170..1057
Translation	MEELEQDLLLQPWAWLQLAENSLLAKVSITKHGYALLISDLQQVWHEQVDTSVVSQRAKELNKRLTAPPAALLCHLDEALRPLFKDSAHPSKATFSCDRGEEGLILRVQSELSGLPFSWHFHCIPASSSLVSQHLIHPLMGVSLALQSHVRELAALLRMKDLEIQAYQESGAVLSRSRLKTEPFEENSFLEQFMAEKLPEACAVGDGKPFAMSLQSLYVAVTKQQIQARQAHKDSGETQASSSTSPRGTDNQPEEPVSLSSTLSEPEYEPVAASGPMHRARLVKSKRKKPRGLFS

Target ORF information:

RefSeq Version	NM_029342.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus non-homologous end joining factor 1 (Nhej1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029342.4

ORF Insert Sequence:

ATGGAAGAGC TAGAGCAAGA CCTGTTGCTG CAGCCATGGG CATGGTTACA ACTTGCGGAG 
AACTCACTCT TAGCCAAGGT GTCTATCACC AAGCACGGTT ATGCCTTGCT GATTTCGGAT 
CTTCAACAGG TGTGGCATGA ACAGGTGGAC ACTTCGGTGG TCAGCCAGCG AGCCAAGGAG 
CTGAACAAGC GCCTCACTGC GCCTCCTGCA GCTTTGCTCT GTCACCTGGA TGAAGCACTT 
CGCCCACTGT TTAAAGATTC TGCTCACCCT AGCAAAGCTA CTTTCTCCTG TGACCGAGGA 
GAGGAGGGAC TGATCCTGCG GGTGCAGAGT GAGCTCTCGG GTCTTCCCTT CAGTTGGCAT 
TTCCACTGTA TTCCAGCTAG TTCTTCACTG GTCTCTCAGC ATTTGATTCA TCCTCTGATG 
GGTGTGAGCC TGGCACTGCA GAGTCATGTG AGGGAGCTAG CAGCATTGCT TCGGATGAAG 
GACCTTGAGA TCCAGGCCTA CCAGGAGAGT GGGGCTGTGC TGAGCCGAAG TCGATTGAAG 
ACAGAGCCAT TTGAAGAAAA TTCTTTCTTG GAACAGTTTA TGGCAGAGAA ATTGCCAGAG 
GCGTGTGCTG TTGGTGATGG AAAGCCATTT GCCATGAGTC TGCAGAGTCT GTATGTGGCA 
GTTACAAAAC AGCAGATCCA AGCAAGGCAG GCACATAAAG ACTCTGGAGA GACTCAGGCA 
TCAAGCAGCA CCTCCCCTCG AGGAACTGAT AACCAGCCAG AAGAGCCGGT CTCCCTCTCT 
TCCACCCTCT CAGAGCCTGA ATATGAGCCT GTGGCTGCTT CAGGCCCTAT GCATAGAGCT 
CGGCTGGTGA AGTCCAAGAG GAAGAAGCCC AGGGGACTCT TCAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PAXX and Xlf interplay revealed by impaired CNS development and immunodeficiency of double KO mice.
Cell death and differentiation25(2)444-452(2018 Feb)
Abramowski V,Etienne O,Elsaid R,Yang J,Berland A,Kermasson L,Roch B,Musilli S,Moussu JP,Lipson-Ruffert K,Revy P,Cumano A,Boussin FD,de Villartay JP

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Nhej1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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