Akr1c21 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Akr1c21
Entrez Gene ID	77337
Full Name	aldo-keto reductase family 1, member C21
Synonyms	9430025F20Rik,AI315367
General protein information	Preferred Names aldo-keto reductase family 1, member C21 Names aldo-keto reductase family 1 member C21 17-alpha-HSD 17-alpha-hydroxysteroid dehydrogenase 3(17)alpha-hydroxysteroid dehydrogenase 3(or 17)-alpha-hydroxysteroid dehydrogenase 3-alpha-hydroxysteroid dehydrogenase DD1 DD3 dihydrodiol dehydrogenase type 1 dihydrodiol dehydrogenase type 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13\|13 A1

mRNA and Protein(s)

mRNA Protein Name

NM_029901.2 NP_084177.2 aldo-keto reductase family 1 member C21
Pathways from BioSystems

mRNA	Protein	Name
NM_029901.2	NP_084177.2	aldo-keto reductase family 1 member C21

Homologies

Homo sapiens (human)	AKR1C1	NP_001344.2
Arabidopsis thaliana (thale cress)	AT1G59950	NP_176203.1
Rattus norvegicus (Norway rat)	Akr1c2	NP_001013075.1
Arabidopsis thaliana (thale cress)	AT1G59960	NP_176204.1
Homo sapiens (human)	AKR1C2	NP_995317.1
Mus musculus (house mouse)	Akr1c21	NP_084177.2
	AKR1C4	XP_001104300.1
Pan troglodytes (chimpanzee)	AKR1C1	XP_003951630.1

Gene Ontology
Bibliography

Related articles in PubMed

Studies on a Tyr residue critical for the binding of coenzyme and substrate in mouse 3(17)alpha-hydroxysteroid dehydrogenase (AKR1C21): structure of the Y224D mutant enzyme.
Dhagat U, Endo S, Mamiya H, Hara A, El-Kabbani O
Acta crystallographica. Section D, Biological crystallography66(Pt 2)198-204(2010 Feb)

Structure of the G225P/G226P mutant of mouse 3(17)alpha-hydroxysteroid dehydrogenase (AKR1C21) ternary complex: implications for the binding of inhibitor and substrate.
Dhagat U, Endo S, Mamiya H, Hara A, El-Kabbani O
Acta crystallographica. Section D, Biological crystallography65(Pt 3)257-65(2009 Mar)

Mouse 17alpha-hydroxysteroid dehydrogenase (AKR1C21) binds steroids differently from other aldo-keto reductases: identification and characterization of amino acid residues critical for substrate binding.
Faucher F, Cantin L, Pereira de J?sus-Tran K, Lemieux M, Luu-The V, Labrie F, Breton R
Journal of molecular biology369(2)525-40(2007 Jun)

Crystal structures of mouse 17alpha-hydroxysteroid dehydrogenase (apoenzyme and enzyme-NADP(H) binary complex): identification of molecular determinants responsible for the unique 17alpha-reductive activity of this enzyme.
Faucher F, Pereira de J?sus-Tran K, Cantin L, Luu-The V, Labrie F, Breton R
Journal of molecular biology364(4)747-63(2006 Dec)

Crystallization and preliminary X-ray diffraction analysis of mouse 3(17)alpha-hydroxysteroid dehydrogenase.
El-Kabbani O, Ishikura S, Wagner A, Schulze-Briese C, Hara A
Acta crystallographica. Section F, Structural biology and crystallization communications61(Pt 7)688-90(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

mutation resulted in a change in the conformation of the flexible loop B, including the V-shaped groove, which is a unique feature of the active-site architecture of wild-type AKR1C21 and is formed by the side chains of Tyr224 and Trp227.
Title: Studies on a Tyr residue critical for the binding of coenzyme and substrate in mouse 3(17)alpha-hydroxysteroid dehydrogenase (AKR1C21): structure of the Y224D mutant enzyme.

Gly225 & Gly226 help determine the substrate stereospecificity. The G225P/G226P mutation reduced the affinity for both 3alpha- & 17alpha-hydroxysteroid substrates by up to 160-fold, indicating that these residues are critical for substrate binding.
Title: Structure of the G225P/G226P mutant of mouse 3(17)alpha-hydroxysteroid dehydrogenase (AKR1C21) ternary complex: implications for the binding of inhibitor and substrate.

study reports a ternary complex crystal structure (m17alpha-HSD:NADP(+):epi-T) determined at 1.85 A resolution reveals a unique steroid-binding mode for an AKR enzyme
Title: Mouse 17alpha-hydroxysteroid dehydrogenase (AKR1C21) binds steroids differently from other aldo-keto reductases: identification and characterization of amino acid residues critical for substrate binding.

crystal structure of the m17alpha-HSD enzyme in its apo-form (1.9 A resolution) as well as those of two different forms of this enzyme in binary complex with NADP(H) (2.9 A and 1.35 A resolution)(
Title: Crystal structures of mouse 17alpha-hydroxysteroid dehydrogenase (apoenzyme and enzyme-NADP(H) binary complex): identification of molecular determinants responsible for the unique 17alpha-reductive activity of this enzyme.

X-ray diffraction analysis of mouse 3(17)alpha-hydroxysteroid dehydrogenase
Title: Crystallization and preliminary X-ray diffraction analysis of mouse 3(17)alpha-hydroxysteroid dehydrogenase.

The following Akr1c21 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Akr1c21 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu07654	NM_029901.2 Latest version!	Mus musculus aldo-keto reductase family 1, member C21 (Akr1c21), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Akr1c21 ( NM_029901.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_084177.2 Mus musculus aldo-keto reductase family 1, member C21 (Akr1c21), mRNA.

Price & Availability↑

CloneID	OMu07654
Clone ID Related Accession (Same CDS sequence)	NM_029901.2
Accession Version	NM_029901.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	aldo-keto reductase family 1 member C21
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY721648.1, AY742217.1, AI882115.1 and AI035317.1. On Feb 23, 2007 this sequence version replaced NM_029901.1. ##Evidence-Data-START## Transcript exon combination :: AK020439.1, BC061057.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTCCA AATGTCATTG TGTCATATTG AATGATGGTA ACTTCATTCC AGTGCTGGGT 
TTTGGTACTG CTCTTCCTCT AGAGTGTCCC AAGAGTAAAG CTAAGGAGCT CACCAAAATA 
GCTATAGATG CTGGTTTCCA TCACTTTGAT TCTGCTTCTG TCTATAATAC TGAAGATCAT 
GTAGGAGAGG CCATCAGAAG CAAGATTGCT GATGGCACTG TAAGGAGAGA AGATATATTT 
TACACCTCAA AGGTTTGGTG TACTAGCCTT CACCCAGAAC TTGTGAGAGC TTCCTTGGAA 
CGGTCACTGC AAAAACTTCA GTTCGACTAT GTGGACCTGT ATCTCATTCA TTACCCAATG 
GCCCTGAAAC CAGGAGAAGA AAATTTTCCA GTAGATGAAC ATGGAAAATT AATATTTGAC 
AGAGTGGACC TCTGTGCCAC CTGGGAGGCC ATGGAGAAGT GTAAGGATGC AGGACTAACC 
AAGTCCATTG GGGTGTCTAA CTTTAACTAT AGACAGTTGG AGATGATTCT GAATAAGCCT 
GGGCTCAAGT ACAAGCCGGT ATGCAACCAG GTAGAATGCC ATCCTTATCT CAACCAAATG 
AAACTTCTGG ATTTCTGCAA ATCAAAAGAT ATTGTATTGG TTGCCTATGG TGTTCTAGGA 
ACACAACGAT ATGGAGGATG GGTAGACCAG AATTCCCCTG TTCTCTTGGA TGAACCAGTT 
CTTGGTTCCA TGGCAAAAAA ATATAATCGA ACTCCAGCCT TGATTGCCCT TCGCTACCAG 
TTACAGCGTG GGATTGTGGT CCTCAACACC AGTCTCAAAG AGGAGCGGAT CAAAGAGAAC 
ATGCAGGTTT TTGAATTCCA GCTGAGTTCA GAGGATATGA AAGTTCTTGA TGGCCTGAAC 
AGAAATATGC GATACATACC TGCTGCCATT TTCAAGGGCC ACCCTAATTG GCCATTTTTG 
GATGAATACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_084177.2
CDS	75..1046
Translation	MNSKCHCVILNDGNFIPVLGFGTALPLECPKSKAKELTKIAIDAGFHHFDSASVYNTEDHVGEAIRSKIADGTVRREDIFYTSKVWCTSLHPELVRASLERSLQKLQFDYVDLYLIHYPMALKPGEENFPVDEHGKLIFDRVDLCATWEAMEKCKDAGLTKSIGVSNFNYRQLEMILNKPGLKYKPVCNQVECHPYLNQMKLLDFCKSKDIVLVAYGVLGTQRYGGWVDQNSPVLLDEPVLGSMAKKYNRTPALIALRYQLQRGIVVLNTSLKEERIKENMQVFEFQLSSEDMKVLDGLNRNMRYIPAAIFKGHPNWPFLDEY

Target ORF information:

RefSeq Version	NM_029901.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus aldo-keto reductase family 1, member C21 (Akr1c21), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029901.2

ORF Insert Sequence:

ATGAACTCCA AATGTCATTG TGTCATATTG AATGATGGTA ACTTCATTCC AGTGCTGGGT 
TTTGGTACTG CTCTTCCTCT AGAGTGTCCC AAGAGTAAAG CTAAGGAGCT CACCAAAATA 
GCTATAGATG CTGGTTTCCA TCACTTTGAT TCTGCTTCTG TCTATAATAC TGAAGATCAT 
GTAGGAGAGG CCATCAGAAG CAAGATTGCT GATGGCACTG TAAGGAGAGA AGATATATTT 
TACACCTCAA AGGTTTGGTG TACTAGCCTT CACCCAGAAC TTGTGAGAGC TTCCTTGGAA 
CGGTCACTGC AAAAACTTCA GTTCGACTAT GTGGACCTGT ATCTCATTCA TTACCCAATG 
GCCCTGAAAC CAGGAGAAGA AAATTTTCCA GTAGATGAAC ATGGAAAATT AATATTTGAC 
AGAGTGGACC TCTGTGCCAC CTGGGAGGCC ATGGAGAAGT GTAAGGATGC AGGACTAACC 
AAGTCCATTG GGGTGTCTAA CTTTAACTAT AGACAGTTGG AGATGATTCT GAATAAGCCT 
GGGCTCAAGT ACAAGCCGGT ATGCAACCAG GTAGAATGCC ATCCTTATCT CAACCAAATG 
AAACTTCTGG ATTTCTGCAA ATCAAAAGAT ATTGTATTGG TTGCCTATGG TGTTCTAGGA 
ACACAACGAT ATGGAGGATG GGTAGACCAG AATTCCCCTG TTCTCTTGGA TGAACCAGTT 
CTTGGTTCCA TGGCAAAAAA ATATAATCGA ACTCCAGCCT TGATTGCCCT TCGCTACCAG 
TTACAGCGTG GGATTGTGGT CCTCAACACC AGTCTCAAAG AGGAGCGGAT CAAAGAGAAC 
ATGCAGGTTT TTGAATTCCA GCTGAGTTCA GAGGATATGA AAGTTCTTGA TGGCCTGAAC 
AGAAATATGC GATACATACC TGCTGCCATT TTCAAGGGCC ACCCTAATTG GCCATTTTTG 
GATGAATACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Studies on a Tyr residue critical for the binding of coenzyme and substrate in mouse 3(17)alpha-hydroxysteroid dehydrogenase (AKR1C21): structure of the Y224D mutant enzyme.
Acta crystallographica. Section D, Biological crystallography66(Pt 2)198-204(2010 Feb)
Dhagat U,Endo S,Mamiya H,Hara A,El-Kabbani O

Structure of the G225P/G226P mutant of mouse 3(17)alpha-hydroxysteroid dehydrogenase (AKR1C21) ternary complex: implications for the binding of inhibitor and substrate.
Acta crystallographica. Section D, Biological crystallography65(Pt 3)257-65(2009 Mar)
Dhagat U,Endo S,Mamiya H,Hara A,El-Kabbani O

Mouse 17alpha-hydroxysteroid dehydrogenase (AKR1C21) binds steroids differently from other aldo-keto reductases: identification and characterization of amino acid residues critical for substrate binding.
Journal of molecular biology369(2)525-40(2007 Jun)
Faucher F,Cantin L,Pereira de J?sus-Tran K,Lemieux M,Luu-The V,Labrie F,Breton R

Crystal structures of mouse 17alpha-hydroxysteroid dehydrogenase (apoenzyme and enzyme-NADP(H) binary complex): identification of molecular determinants responsible for the unique 17alpha-reductive activity of this enzyme.
Journal of molecular biology364(4)747-63(2006 Dec)
Faucher F,Pereira de J?sus-Tran K,Cantin L,Luu-The V,Labrie F,Breton R

Crystallization and preliminary X-ray diffraction analysis of mouse 3(17)alpha-hydroxysteroid dehydrogenase.
Acta crystallographica. Section F, Structural biology and crystallization communications61(Pt 7)688-90(2005 Jul)
El-Kabbani O,Ishikura S,Wagner A,Schulze-Briese C,Hara A

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Akr1c21 cDNA ORF clone, Mus musculus(house mouse)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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