HECW1 cDNA ORF clone, Ornithorhynchus anatinus(platypus)

Gene
Clones
gRNAs

Summary

Gene Symbol	HECW1
Entrez Gene ID	100076282
Full Name	HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1
Gene Type	protein-coding
Organism	Ornithorhynchus anatinus(platypus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029063052.1	XP_028918885.1	E3 ubiquitin-protein ligase HECW1 isoform X1
XM_029063058.1	XP_028918891.1	E3 ubiquitin-protein ligase HECW1 isoform X5
XM_029063057.1	XP_028918890.1	E3 ubiquitin-protein ligase HECW1 isoform X4
XM_029063054.1	XP_028918887.1	E3 ubiquitin-protein ligase HECW1 isoform X2
XM_029063056.1	XP_028918889.1	E3 ubiquitin-protein ligase HECW1 isoform X3
XM_007663451.1	XP_007661641.1	E3 ubiquitin-protein ligase HECW1
XM_029063051.1	XP_028918884.1	E3 ubiquitin-protein ligase HECW1 isoform X1
XM_029063053.1	XP_028918886.1	E3 ubiquitin-protein ligase HECW1 isoform X1

Pathways from BioSystems
Gene Ontology

The following HECW1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECW1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOc127718	XM_029063058.1 Latest version!	Ornithorhynchus anatinus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OOc127714	XM_029063053.1 Latest version!	Ornithorhynchus anatinus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OOc127714	XM_029063052.1 Latest version!	Ornithorhynchus anatinus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OOc127714	XM_029063051.1 Latest version!	Ornithorhynchus anatinus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OOc127715	XM_029063054.1 Latest version!	Ornithorhynchus anatinus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OOc127716	XM_029063056.1 Latest version!	Ornithorhynchus anatinus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OOc127717	XM_029063057.1 Latest version!	Ornithorhynchus anatinus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OOc15631	XM_007663451.1 Latest version!	Ornithorhynchus anatinus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HECW1 ( XM_029063058.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028918891.1 Ornithorhynchus anatinus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OOc127718
Clone ID Related Accession (Same CDS sequence)	XM_029063058.1
Accession Version	XM_029063058.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	E3 ubiquitin-protein ligase HECW1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041731.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACCA TCGCCCCCAC TAGAGGTGAT TGCATTGCAG AGCAGGTTTT TAAGAGCATC 
ACCAATGACG AGGCCACCCA GGGTCAAGGC AGTCGGAGGC TGATCAGTTT TTCTCTCTCA 
GATTTCCAGG CCATGGGTCT GAAGAAAGGA ATGTTCTTTA ACCCAGATCC ATATCTGAAG 
ATTTCCATCC AACCTGGGAA ACACAGCATC TTCCCAGCCC TTCCGCACCA TGGTCAGGAG 
AAGAGGTCGA AGATTACCTG TAACACCGTG AACCCTGTTT GGCAAGCTGA GCAGTTTAGC 
TTTGTTTCCC TGCCCACCGA CGTCCTAGAA ATCGAGGTGA AAGACAAGTT TGCCAAGAGT 
CGTCCAATCA TCAAACGCTT CTTAGGAAAG TTGTCCATGC CTGTTCAGCG ACTCCTGGAG 
AGACACGCTA TAGGGGACAG GGTGGTGAGC TACACTCTGG GTCGAAGGCT TCCAACGGAT 
CACGTCAGCG GCCAGCTCCA GTTCCGGTTC GAGATCACCT CCTCCATCCA CCCAGATGAC 
GAGGAGGTCT CTCTGAGCGC AGATCCCGAG TCTGCTGAAA ACCAGGAGAG TCCTGCCAAC 
CACCCATCAG AGGAAGGCCT CACAGAACCT CCAGGCCAGG GGACGGAGGG CCCAGAGGGC 
CCGGAGCTGG AGACACAGGA TGGTGGTGGT CCCACGAGTG GCTCTGGGGG AGACAGCCCC 
GAGGCTACCG GACCGGCCGG GGAAAAGTCG AATGAGGAGG AGGCCGCAGA GGGTGCCGAA 
GCCGTCGTGG AGCCAGTTGA TGCCGTCGAT CCCACGGAAC CCAGCCCGGC CGAAGATTTG 
CCTTCCCTCA GTGCTGAAGA GATCGCCGAG GGGCTGGATC TGCTCCCTGG CACTCCCCCG 
CCACCGCCTC CGGAAGACGA GGGTTGGGAG CAGGGGGAGA ACCACGCCGG AGAGGCCGAT 
GGAGATCCCC TTGTCGGCCT GGAGGAGCCA GAGGGGGCTG AGGCAGAGGT GGAGAAAAAG 
GTTGACGTGA GCATGGTTCT AGACAAAGAC CAGGATGAAG AGGAAGAAGA ATTGGCCTCC 
CCCGAAGAGG ATGGGCCTCT GGCGCTGAGA CCCTCGGCCA AGAAGAAAAG CAGACCGTGC 
TCCCTGCCCG TGTCCGAGTT GGAGACGGTG ATCGCGTCGG CCTGCGGCGA ACCGGAAACG 
CCCCGCACCC ATTACATCCG CATCCACAAC CTGCTGCACA GCATGCCCTC AGCCCAGGGA 
GGCAGCGATG GCGGAGAAGC CCAAGGACCG GAGGAAGAGG ACGAGGAGGG GCCGGGGGAG 
GAAGAGTCAA CGGTCAAGGA GGCCTCCGAA AAGGACGTGG TCAGCGAGGC CGAGACCGTG 
GCACCGGACC CACCTCACCC AGGAGATGGC GGAGAGAGCG GGGACCCCGA GGTATCTGGC 
CAGCGGCCGG TGCCCCACAG TGCCTCGGCC GAGTGCTTGG CCGGGGTGAA CGGTGGCCCG 
CTTCTCGACG GCGAGCCCCG GCCCAGCACG GGGAGCGAGA GCGACTCGAG CCCCCGGCAG 
GACTGCGAGG GCTGCGACAC ATCCTGCTAC AGCCCGTCCT GCTACAGCAC CTCGTGTTAC 
AGCACCTCCT GCTACAGCAC CTCGTGCTAC AGCACCTCCT GCTACAGCCC ATCCTGCTAC 
GACAGCAACA ACCGGTTCTC CAGCCATACG CGCTTCTCCT CGGTGGACAG TGCCAAGATC 
TCGGAGAGCA CCGTCTTCTC CTCTCAGGAC GAGGAGGAGG AGGAGAACAG CGCGTTCGAG 
TCGGTGCCCG ACTCGGTGCA GAGCCCGGAG ATGGACCGGG AGACGGCCGA CGGCACCGGC 
CCGTGGCCGG ACGAGCTGGT TCTCCCCAAC GGGAACGTTC CGAGGACAGC CGATGGCCTG 
GAGTCCCCGA TAGCTGGTCC CAGTAATCGG AGAGAAGCTG AAGTACTACA TTTTCATCAT 
ATCATTTCGT CGGCTTGGAT TTCACTAGCA GGTGATTGTC CTTTACTACA TAATTCTCAG 
CCAGTCAGCC AGCTTCCTTC CTTGAGGCCT GACCACCACC ATTATCCAAC TATCGATGAG 
CCTCTTCCGC CAAACTGGGA GGCTCGAATA GACAGTCACG GGAGAGTGTT TTACGTGGAC 
CACGTGAACC GAACGACGAC GTGGCAGCGG CCTACCACGG CCGCCACTCC AGACGGGATC 
CGCAGATCTG GCTCCGTACA GCAGATGGAA CAGCTGAACC GCCGGTACCA GAATATCCAG 
CGGACTATTG CTACCGAGAG ATCGGAAGAC GAGCCACCCA GTCAGAGTGC GGAGCCTCTC 
CCTGCCGGAG GAGGGAGTGG AGGAGGGGGA GGAGGGAGTG ACTTGGAAGC AGACTCTTCT 
CAACCAAATC TAGAACTGAG GAGAGAGGGG TCTCTTTCTC CTGTGAACTC CCAAAAAATC 
ACTTTGCTGC TTCAGTCCCC GGCCGTCAAG TTCATCACCA ACCCTGAGTT CTTCACCGTG 
CTGCATGCCA ACTATAGTGC CTATAGAGTC TTCACCAACA GCACCTGCTT GAAGCATATG 
ATTCTGAAGA TCCGCAGGGA TGCCCGGAAT TTCGAACGCT ACCAGCACAA CCGGGACCTG 
GTGAACTTCA TCAACATGTT TGCTGACACC CGTTTGGAAC TGCCTCGCGG CTGGGAAATC 
AAAACTGACC AGCAGGGCAA GTCCTTCTTC GTCGACCACA ACAGTCGAGC CACCACTTTC 
ATCGACCCCC GGATCCCTCT TCAGAACGGA CGTCTTCCGA ATCACCTGAC TCACCGGCAG 
CACCTTCAGC GACTCCGGAG CTACAGTGCC GGCGAGGCCT CAGAAGTCTC TCGGAACCGA 
GGAGCTTCTC TGTTGGCCAG GCCGGGTCAC AGCCTGGTAG CAGCCATCAG AAACCAACAT 
CACCATGAGA CGTTACCTCT GGCTTATAAT GACAAGATCG TGGCATTTCT CCGCCAGCCA 
AACATTTTTG AGATGCTGCA GGAGCGCCAG CCCAGCTTGG CCAGAAACCA CGCACTCAGG 
GAGAAAATCC ATTACATTCG GACAGAAGGT ACTCAAGGGC TTGAAAAATT GTCCTGTGAT 
GCAGATTTAG TAATACTGCT GAGCCTCTTT GAAGAAGAGA TCATGTCCTA CATACCCCTT 
CAGGCCGCTT TCCACCCGGG ATACAGCTTC TCTCCTCGCT GTTCACCGTG CTCTTCCCCT 
CAAAACTCTC CAGGCCTACA GCGAGCCAGT GTCAGGGCCC CCTCACCCTA CAGGAGAGAC 
TTTGAGGCCA AGCTCCGAAA TTTCTACCGA AAACTTGAAG CCAAAGGATT TGGTCAAGGT 
CCGGGTAAAA TCAAACTCAT CATAAGACGT GACCACTTGC TGGAGGGAAC CTTCAATCAG 
GTGATGGCTT ATTCCCGCAA AGAGCTCCAG CGGAACAAGC TCTACGTCAC ATTTGTTGGA 
GAGGAGGGCC TAGACTACAG TGGGCCCTCC CGGGAGTTCT TTTTCCTTTT GTCCCAGGAG 
CTCTTCAACC CCTACTACGG CCTGTTTGAG TATTCTGCCA ATGACACTTA CACCGTGCAG 
ATCAGCCCCA TGTCGGCCTT TGTGGAAAAT CATCTCGAGT GGTTCAGATT TAGCGGTCGC 
ATCCTTGGGC TTGCCCTGAT CCACCAGTAC CTCCTGGACG CTTTCTTCAC GAGGCCGTTC 
TACAAGGCCC TGCTGAGACT GCCCTGTGAT CTGAGCGACC TAGAGTATCT GGATGAGGAA 
TTCCACCAAA GCCTGCAGTG GATGAAGGAC AATAACATCA CGGATATCTT AGATCTCACT 
TTCACAGTGA ATGAAGAAGT GTTTGGACAG GTAACAGAAA GAGAGTTGAA ATCAGGGGGA 
GCCAACACCC AGGTGACAGA AAAAAATAAA AAGGAGTACA TTGAGAGAAT GGTGAAGTGG 
AGAGTGGAGC GGGGCGTGGT GCAGCAGACG GAAGCGTTAG TCCGAGGCTT CTATGAAGTT 
GTGGATTCAA GGCTCGTCTC CGTCTTTGAT GCCAGGGAGC TGGAGCTGGT GATCGCCGGC 
ACCGCCGAAA TAGACCTCAA TGACTGGCGC AATAACACCG AGTATCGAGG GGGTTATCAC 
GATGGTCATA TCGTGATCCG GTGGTTCTGG GCAGCTGTGG AGAGGTTCAA CAATGAGCAG 
AGACTGAGGT TGCTTCAGTT CGTCACGGGA ACATCGAGCG TACCCTATGA AGGTTTCGCC 
GCGCTCCGAG GAAGCAATGG GCTGAGGCGC TTCTGTATAG AGAAATGGGG GAAAATAACA 
TCCCTTCCCA GGGCACACAC GTGTTTCAAC CGCTTGGATC TGCCACCGTA CCCATCGTAT 
TCCATGCTGT ACGAGAAGCT GTTGACAGCA GTTGAGGAAA CGAGCACCTT TGGTCTGGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028918891.1
CDS	54..4496
Translation	MATIAPTRGDCIAEQVFKSITNDEATQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQEKRSKITCNTVNPVWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEVSLSADPESAENQESPANHPSEEGLTEPPGQGTEGPEGPELETQDGGGPTSGSGGDSPEATGPAGEKSNEEEAAEGAEAVVEPVDAVDPTEPSPAEDLPSLSAEEIAEGLDLLPGTPPPPPPEDEGWEQGENHAGEADGDPLVGLEEPEGAEAEVEKKVDVSMVLDKDQDEEEEELASPEEDGPLALRPSAKKKSRPCSLPVSELETVIASACGEPETPRTHYIRIHNLLHSMPSAQGGSDGGEAQGPEEEDEEGPGEEESTVKEASEKDVVSEAETVAPDPPHPGDGGESGDPEVSGQRPVPHSASAECLAGVNGGPLLDGEPRPSTGSESDSSPRQDCEGCDTSCYSPSCYSTSCYSTSCYSTSCYSTSCYSPSCYDSNNRFSSHTRFSSVDSAKISESTVFSSQDEEEEENSAFESVPDSVQSPEMDRETADGTGPWPDELVLPNGNVPRTADGLESPIAGPSNRREAEVLHFHHIISSAWISLAGDCPLLHNSQPVSQLPSLRPDHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTTAATPDGIRRSGSVQQMEQLNRRYQNIQRTIATERSEDEPPSQSAEPLPAGGGSGGGGGGSDLEADSSQPNLELRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTNSTCLKHMILKIRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRNQHHHETLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHALREKIHYIRTEGTQGLEKLSCDADLVILLSLFEEEIMSYIPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASVRAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_029063058.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029063058.1

ORF Insert Sequence:

ATGGCTACCA TCGCCCCCAC TAGAGGTGAT TGCATTGCAG AGCAGGTTTT TAAGAGCATC 
ACCAATGACG AGGCCACCCA GGGTCAAGGC AGTCGGAGGC TGATCAGTTT TTCTCTCTCA 
GATTTCCAGG CCATGGGTCT GAAGAAAGGA ATGTTCTTTA ACCCAGATCC ATATCTGAAG 
ATTTCCATCC AACCTGGGAA ACACAGCATC TTCCCAGCCC TTCCGCACCA TGGTCAGGAG 
AAGAGGTCGA AGATTACCTG TAACACCGTG AACCCTGTTT GGCAAGCTGA GCAGTTTAGC 
TTTGTTTCCC TGCCCACCGA CGTCCTAGAA ATCGAGGTGA AAGACAAGTT TGCCAAGAGT 
CGTCCAATCA TCAAACGCTT CTTAGGAAAG TTGTCCATGC CTGTTCAGCG ACTCCTGGAG 
AGACACGCTA TAGGGGACAG GGTGGTGAGC TACACTCTGG GTCGAAGGCT TCCAACGGAT 
CACGTCAGCG GCCAGCTCCA GTTCCGGTTC GAGATCACCT CCTCCATCCA CCCAGATGAC 
GAGGAGGTCT CTCTGAGCGC AGATCCCGAG TCTGCTGAAA ACCAGGAGAG TCCTGCCAAC 
CACCCATCAG AGGAAGGCCT CACAGAACCT CCAGGCCAGG GGACGGAGGG CCCAGAGGGC 
CCGGAGCTGG AGACACAGGA TGGTGGTGGT CCCACGAGTG GCTCTGGGGG AGACAGCCCC 
GAGGCTACCG GACCGGCCGG GGAAAAGTCG AATGAGGAGG AGGCCGCAGA GGGTGCCGAA 
GCCGTCGTGG AGCCAGTTGA TGCCGTCGAT CCCACGGAAC CCAGCCCGGC CGAAGATTTG 
CCTTCCCTCA GTGCTGAAGA GATCGCCGAG GGGCTGGATC TGCTCCCTGG CACTCCCCCG 
CCACCGCCTC CGGAAGACGA GGGTTGGGAG CAGGGGGAGA ACCACGCCGG AGAGGCCGAT 
GGAGATCCCC TTGTCGGCCT GGAGGAGCCA GAGGGGGCTG AGGCAGAGGT GGAGAAAAAG 
GTTGACGTGA GCATGGTTCT AGACAAAGAC CAGGATGAAG AGGAAGAAGA ATTGGCCTCC 
CCCGAAGAGG ATGGGCCTCT GGCGCTGAGA CCCTCGGCCA AGAAGAAAAG CAGACCGTGC 
TCCCTGCCCG TGTCCGAGTT GGAGACGGTG ATCGCGTCGG CCTGCGGCGA ACCGGAAACG 
CCCCGCACCC ATTACATCCG CATCCACAAC CTGCTGCACA GCATGCCCTC AGCCCAGGGA 
GGCAGCGATG GCGGAGAAGC CCAAGGACCG GAGGAAGAGG ACGAGGAGGG GCCGGGGGAG 
GAAGAGTCAA CGGTCAAGGA GGCCTCCGAA AAGGACGTGG TCAGCGAGGC CGAGACCGTG 
GCACCGGACC CACCTCACCC AGGAGATGGC GGAGAGAGCG GGGACCCCGA GGTATCTGGC 
CAGCGGCCGG TGCCCCACAG TGCCTCGGCC GAGTGCTTGG CCGGGGTGAA CGGTGGCCCG 
CTTCTCGACG GCGAGCCCCG GCCCAGCACG GGGAGCGAGA GCGACTCGAG CCCCCGGCAG 
GACTGCGAGG GCTGCGACAC ATCCTGCTAC AGCCCGTCCT GCTACAGCAC CTCGTGTTAC 
AGCACCTCCT GCTACAGCAC CTCGTGCTAC AGCACCTCCT GCTACAGCCC ATCCTGCTAC 
GACAGCAACA ACCGGTTCTC CAGCCATACG CGCTTCTCCT CGGTGGACAG TGCCAAGATC 
TCGGAGAGCA CCGTCTTCTC CTCTCAGGAC GAGGAGGAGG AGGAGAACAG CGCGTTCGAG 
TCGGTGCCCG ACTCGGTGCA GAGCCCGGAG ATGGACCGGG AGACGGCCGA CGGCACCGGC 
CCGTGGCCGG ACGAGCTGGT TCTCCCCAAC GGGAACGTTC CGAGGACAGC CGATGGCCTG 
GAGTCCCCGA TAGCTGGTCC CAGTAATCGG AGAGAAGCTG AAGTACTACA TTTTCATCAT 
ATCATTTCGT CGGCTTGGAT TTCACTAGCA GGTGATTGTC CTTTACTACA TAATTCTCAG 
CCAGTCAGCC AGCTTCCTTC CTTGAGGCCT GACCACCACC ATTATCCAAC TATCGATGAG 
CCTCTTCCGC CAAACTGGGA GGCTCGAATA GACAGTCACG GGAGAGTGTT TTACGTGGAC 
CACGTGAACC GAACGACGAC GTGGCAGCGG CCTACCACGG CCGCCACTCC AGACGGGATC 
CGCAGATCTG GCTCCGTACA GCAGATGGAA CAGCTGAACC GCCGGTACCA GAATATCCAG 
CGGACTATTG CTACCGAGAG ATCGGAAGAC GAGCCACCCA GTCAGAGTGC GGAGCCTCTC 
CCTGCCGGAG GAGGGAGTGG AGGAGGGGGA GGAGGGAGTG ACTTGGAAGC AGACTCTTCT 
CAACCAAATC TAGAACTGAG GAGAGAGGGG TCTCTTTCTC CTGTGAACTC CCAAAAAATC 
ACTTTGCTGC TTCAGTCCCC GGCCGTCAAG TTCATCACCA ACCCTGAGTT CTTCACCGTG 
CTGCATGCCA ACTATAGTGC CTATAGAGTC TTCACCAACA GCACCTGCTT GAAGCATATG 
ATTCTGAAGA TCCGCAGGGA TGCCCGGAAT TTCGAACGCT ACCAGCACAA CCGGGACCTG 
GTGAACTTCA TCAACATGTT TGCTGACACC CGTTTGGAAC TGCCTCGCGG CTGGGAAATC 
AAAACTGACC AGCAGGGCAA GTCCTTCTTC GTCGACCACA ACAGTCGAGC CACCACTTTC 
ATCGACCCCC GGATCCCTCT TCAGAACGGA CGTCTTCCGA ATCACCTGAC TCACCGGCAG 
CACCTTCAGC GACTCCGGAG CTACAGTGCC GGCGAGGCCT CAGAAGTCTC TCGGAACCGA 
GGAGCTTCTC TGTTGGCCAG GCCGGGTCAC AGCCTGGTAG CAGCCATCAG AAACCAACAT 
CACCATGAGA CGTTACCTCT GGCTTATAAT GACAAGATCG TGGCATTTCT CCGCCAGCCA 
AACATTTTTG AGATGCTGCA GGAGCGCCAG CCCAGCTTGG CCAGAAACCA CGCACTCAGG 
GAGAAAATCC ATTACATTCG GACAGAAGGT ACTCAAGGGC TTGAAAAATT GTCCTGTGAT 
GCAGATTTAG TAATACTGCT GAGCCTCTTT GAAGAAGAGA TCATGTCCTA CATACCCCTT 
CAGGCCGCTT TCCACCCGGG ATACAGCTTC TCTCCTCGCT GTTCACCGTG CTCTTCCCCT 
CAAAACTCTC CAGGCCTACA GCGAGCCAGT GTCAGGGCCC CCTCACCCTA CAGGAGAGAC 
TTTGAGGCCA AGCTCCGAAA TTTCTACCGA AAACTTGAAG CCAAAGGATT TGGTCAAGGT 
CCGGGTAAAA TCAAACTCAT CATAAGACGT GACCACTTGC TGGAGGGAAC CTTCAATCAG 
GTGATGGCTT ATTCCCGCAA AGAGCTCCAG CGGAACAAGC TCTACGTCAC ATTTGTTGGA 
GAGGAGGGCC TAGACTACAG TGGGCCCTCC CGGGAGTTCT TTTTCCTTTT GTCCCAGGAG 
CTCTTCAACC CCTACTACGG CCTGTTTGAG TATTCTGCCA ATGACACTTA CACCGTGCAG 
ATCAGCCCCA TGTCGGCCTT TGTGGAAAAT CATCTCGAGT GGTTCAGATT TAGCGGTCGC 
ATCCTTGGGC TTGCCCTGAT CCACCAGTAC CTCCTGGACG CTTTCTTCAC GAGGCCGTTC 
TACAAGGCCC TGCTGAGACT GCCCTGTGAT CTGAGCGACC TAGAGTATCT GGATGAGGAA 
TTCCACCAAA GCCTGCAGTG GATGAAGGAC AATAACATCA CGGATATCTT AGATCTCACT 
TTCACAGTGA ATGAAGAAGT GTTTGGACAG GTAACAGAAA GAGAGTTGAA ATCAGGGGGA 
GCCAACACCC AGGTGACAGA AAAAAATAAA AAGGAGTACA TTGAGAGAAT GGTGAAGTGG 
AGAGTGGAGC GGGGCGTGGT GCAGCAGACG GAAGCGTTAG TCCGAGGCTT CTATGAAGTT 
GTGGATTCAA GGCTCGTCTC CGTCTTTGAT GCCAGGGAGC TGGAGCTGGT GATCGCCGGC 
ACCGCCGAAA TAGACCTCAA TGACTGGCGC AATAACACCG AGTATCGAGG GGGTTATCAC 
GATGGTCATA TCGTGATCCG GTGGTTCTGG GCAGCTGTGG AGAGGTTCAA CAATGAGCAG 
AGACTGAGGT TGCTTCAGTT CGTCACGGGA ACATCGAGCG TACCCTATGA AGGTTTCGCC 
GCGCTCCGAG GAAGCAATGG GCTGAGGCGC TTCTGTATAG AGAAATGGGG GAAAATAACA 
TCCCTTCCCA GGGCACACAC GTGTTTCAAC CGCTTGGATC TGCCACCGTA CCCATCGTAT 
TCCATGCTGT ACGAGAAGCT GTTGACAGCA GTTGAGGAAA CGAGCACCTT TGGTCTGGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_029063052.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028918885.1 Ornithorhynchus anatinus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOc127714
Clone ID Related Accession (Same CDS sequence)	XM_029063052.1 , XM_029063051.1 , XM_029063053.1
Accession Version	XM_029063052.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	E3 ubiquitin-protein ligase HECW1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041731.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTTAAGAAT CTGTACCAGA ACAGGTTCCT GGGTCTGGCC 
GCCATGGCCT CGCCGTCGAG GAACTCCCAG AGCCGGCGCC GCTGTAAAGA GCCCCTGCGC 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACATCA GGAACGGCCC ACACGGGACC 
GTCACCATCC CCCGCTCCAC CAGCGACACC GACCTGGTCA CGTCCGACAG TCGGTCCACC 
CTTATGGTCA GCAGTTCGTA TTACTCCATT GGACACTCTC AGGACCTGGT GATCTATTGG 
GATATAAAAG AGGAAGTGGG AGCCGGGGAC TGGATTGGCA TGTATCTCAT CGATGAGGTC 
TTATCTGAAA ATTTTCTGGA CTACAAAAAC CGCGGAGTGA ATGGTTCTCA CCGGGGCCAG 
ATTATCTGGA AGATTGATGC AAGTTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTATC ATGGAGTGAG TGGAGCGCTA CGAGCCACTA CGCCCAGTGT TACAGTGAAA 
AACTCTTCAG CTCCTGTTTT TAAGAGCATC ACCAATGACG AGGCCACCCA GGGTCAAGGC 
AGTCGGAGGC TGATCAGTTT TTCTCTCTCA GATTTCCAGG CCATGGGTCT GAAGAAAGGA 
ATGTTCTTTA ACCCAGATCC ATATCTGAAG ATTTCCATCC AACCTGGGAA ACACAGCATC 
TTCCCAGCCC TTCCGCACCA TGGTCAGGAG AAGAGGTCGA AGATTACCTG TAACACCGTG 
AACCCTGTTT GGCAAGCTGA GCAGTTTAGC TTTGTTTCCC TGCCCACCGA CGTCCTAGAA 
ATCGAGGTGA AAGACAAGTT TGCCAAGAGT CGTCCAATCA TCAAACGCTT CTTAGGAAAG 
TTGTCCATGC CTGTTCAGCG ACTCCTGGAG AGACACGCTA TAGGGGACAG GGTGGTGAGC 
TACACTCTGG GTCGAAGGCT TCCAACGGAT CACGTCAGCG GCCAGCTCCA GTTCCGGTTC 
GAGATCACCT CCTCCATCCA CCCAGATGAC GAGGAGGTCT CTCTGAGCGC AGATCCCGAG 
TCTGCTGAAA ACCAGGAGAG TCCTGCCAAC CACCCATCAG AGGAAGGCCT CACAGAACCT 
CCAGGCCAGG GGACGGAGGG CCCAGAGGGC CCGGAGCTGG AGACACAGGA TGGTGGTGGT 
CCCACGAGTG GCTCTGGGGG AGACAGCCCC GAGGCTACCG GACCGGCCGG GGAAAAGTCG 
AATGAGGAGG AGGCCGCAGA GGGTGCCGAA GCCGTCGTGG AGCCAGTTGA TGCCGTCGAT 
CCCACGGAAC CCAGCCCGGC CGAAGATTTG CCTTCCCTCA GTGCTGAAGA GATCGCCGAG 
GGGCTGGATC TGCTCCCTGG CACTCCCCCG CCACCGCCTC CGGAAGACGA GGGTTGGGAG 
CAGGGGGAGA ACCACGCCGG AGAGGCCGAT GGAGATCCCC TTGTCGGCCT GGAGGAGCCA 
GAGGGGGCTG AGGCAGAGGT GGAGAAAAAG GTTGACGTGA GCATGGTTCT AGACAAAGAC 
CAGGATGAAG AGGAAGAAGA ATTGGCCTCC CCCGAAGAGG ATGGGCCTCT GGCGCTGAGA 
CCCTCGGCCA AGAAGAAAAG CAGACCGTGC TCCCTGCCCG TGTCCGAGTT GGAGACGGTG 
ATCGCGTCGG CCTGCGGCGA ACCGGAAACG CCCCGCACCC ATTACATCCG CATCCACAAC 
CTGCTGCACA GCATGCCCTC AGCCCAGGGA GGCAGCGATG GCGGAGAAGC CCAAGGACCG 
GAGGAAGAGG ACGAGGAGGG GCCGGGGGAG GAAGAGTCAA CGGTCAAGGA GGCCTCCGAA 
AAGGACGTGG TCAGCGAGGC CGAGACCGTG GCACCGGACC CACCTCACCC AGGAGATGGC 
GGAGAGAGCG GGGACCCCGA GGTATCTGGC CAGCGGCCGG TGCCCCACAG TGCCTCGGCC 
GAGTGCTTGG CCGGGGTGAA CGGTGGCCCG CTTCTCGACG GCGAGCCCCG GCCCAGCACG 
GGGAGCGAGA GCGACTCGAG CCCCCGGCAG GACTGCGAGG GCTGCGACAC ATCCTGCTAC 
AGCCCGTCCT GCTACAGCAC CTCGTGTTAC AGCACCTCCT GCTACAGCAC CTCGTGCTAC 
AGCACCTCCT GCTACAGCCC ATCCTGCTAC GACAGCAACA ACCGGTTCTC CAGCCATACG 
CGCTTCTCCT CGGTGGACAG TGCCAAGATC TCGGAGAGCA CCGTCTTCTC CTCTCAGGAC 
GAGGAGGAGG AGGAGAACAG CGCGTTCGAG TCGGTGCCCG ACTCGGTGCA GAGCCCGGAG 
ATGGACCGGG AGACGGCCGA CGGCACCGGC CCGTGGCCGG ACGAGCTGGT TCTCCCCAAC 
GGGAACGTTC CGAGGACAGC CGATGGCCTG GAGTCCCCGA TAGCTGGTCC CAGTAATCGG 
AGAGAAGCTG AAGTACTACA TTTTCATCAT ATCATTTCGT CGGCTTGGAT TTCACTAGCA 
GGTGATTGTC CTTTACTACA TAATTCTCAG CCAGTCAGCC AGCTTCCTTC CTTGAGGCCT 
GACCACCACC ATTATCCAAC TATCGATGAG CCTCTTCCGC CAAACTGGGA GGCTCGAATA 
GACAGTCACG GGAGAGTGTT TTACGTGGAC CACGTGAACC GAACGACGAC GTGGCAGCGG 
CCTACCACGG CCGCCACTCC AGACGGGATC CGCAGATCTG GCTCCGTACA GCAGATGGAA 
CAGCTGAACC GCCGGTACCA GAATATCCAG CGGACTATTG CTACCGAGAG ATCGGAAGAC 
GAGCCACCCA GTCAGAGTGC GGAGCCTCTC CCTGCCGGAG GAGGGAGTGG AGGAGGGGGA 
GGAGGGAGTG ACTTGGAAGC AGACTCTTCT CAACCAAATC TAGAACTGAG GAGAGAGGGG 
TCTCTTTCTC CTGTGAACTC CCAAAAAATC ACTTTGCTGC TTCAGTCCCC GGCCGTCAAG 
TTCATCACCA ACCCTGAGTT CTTCACCGTG CTGCATGCCA ACTATAGTGC CTATAGAGTC 
TTCACCAACA GCACCTGCTT GAAGCATATG ATTCTGAAGA TCCGCAGGGA TGCCCGGAAT 
TTCGAACGCT ACCAGCACAA CCGGGACCTG GTGAACTTCA TCAACATGTT TGCTGACACC 
CGTTTGGAAC TGCCTCGCGG CTGGGAAATC AAAACTGACC AGCAGGGCAA GTCCTTCTTC 
GTCGACCACA ACAGTCGAGC CACCACTTTC ATCGACCCCC GGATCCCTCT TCAGAACGGA 
CGTCTTCCGA ATCACCTGAC TCACCGGCAG CACCTTCAGC GACTCCGGAG CTACAGTGCC 
GGCGAGGCCT CAGAAGTCTC TCGGAACCGA GGAGCTTCTC TGTTGGCCAG GCCGGGTCAC 
AGCCTGGTAG CAGCCATCAG AAACCAACAT CACCATGAGA CGTTACCTCT GGCTTATAAT 
GACAAGATCG TGGCATTTCT CCGCCAGCCA AACATTTTTG AGATGCTGCA GGAGCGCCAG 
CCCAGCTTGG CCAGAAACCA CGCACTCAGG GAGAAAATCC ATTACATTCG GACAGAAGGT 
ACTCAAGGGC TTGAAAAATT GTCCTGTGAT GCAGATTTAG TAATACTGCT GAGCCTCTTT 
GAAGAAGAGA TCATGTCCTA CATACCCCTT CAGGCCGCTT TCCACCCGGG ATACAGCTTC 
TCTCCTCGCT GTTCACCGTG CTCTTCCCCT CAAAACTCTC CAGGCCTACA GCGAGCCAGT 
GTCAGGGCCC CCTCACCCTA CAGGAGAGAC TTTGAGGCCA AGCTCCGAAA TTTCTACCGA 
AAACTTGAAG CCAAAGGATT TGGTCAAGGT CCGGGTAAAA TCAAACTCAT CATAAGACGT 
GACCACTTGC TGGAGGGAAC CTTCAATCAG GTGATGGCTT ATTCCCGCAA AGAGCTCCAG 
CGGAACAAGC TCTACGTCAC ATTTGTTGGA GAGGAGGGCC TAGACTACAG TGGGCCCTCC 
CGGGAGTTCT TTTTCCTTTT GTCCCAGGAG CTCTTCAACC CCTACTACGG CCTGTTTGAG 
TATTCTGCCA ATGACACTTA CACCGTGCAG ATCAGCCCCA TGTCGGCCTT TGTGGAAAAT 
CATCTCGAGT GGTTCAGATT TAGCGGTCGC ATCCTTGGGC TTGCCCTGAT CCACCAGTAC 
CTCCTGGACG CTTTCTTCAC GAGGCCGTTC TACAAGGCCC TGCTGAGACT GCCCTGTGAT 
CTGAGCGACC TAGAGTATCT GGATGAGGAA TTCCACCAAA GCCTGCAGTG GATGAAGGAC 
AATAACATCA CGGATATCTT AGATCTCACT TTCACAGTGA ATGAAGAAGT GTTTGGACAG 
GTAACAGAAA GAGAGTTGAA ATCAGGGGGA GCCAACACCC AGGTGACAGA AAAAAATAAA 
AAGGAGTACA TTGAGAGAAT GGTGAAGTGG AGAGTGGAGC GGGGCGTGGT GCAGCAGACG 
GAAGCGTTAG TCCGAGGCTT CTATGAAGTT GTGGATTCAA GGCTCGTCTC CGTCTTTGAT 
GCCAGGGAGC TGGAGCTGGT GATCGCCGGC ACCGCCGAAA TAGACCTCAA TGACTGGCGC 
AATAACACCG AGTATCGAGG GGGTTATCAC GATGGTCATA TCGTGATCCG GTGGTTCTGG 
GCAGCTGTGG AGAGGTTCAA CAATGAGCAG AGACTGAGGT TGCTTCAGTT CGTCACGGGA 
ACATCGAGCG TACCCTATGA AGGTTTCGCC GCGCTCCGAG GAAGCAATGG GCTGAGGCGC 
TTCTGTATAG AGAAATGGGG GAAAATAACA TCCCTTCCCA GGGCACACAC GTGTTTCAAC 
CGCTTGGATC TGCCACCGTA CCCATCGTAT TCCATGCTGT ACGAGAAGCT GTTGACAGCA 
GTTGAGGAAA CGAGCACCTT TGGTCTGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028918885.1
CDS	265..5217
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDIRNGPHGTVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIYWDIKEEVGAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSSAPVFKSITNDEATQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQEKRSKITCNTVNPVWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEVSLSADPESAENQESPANHPSEEGLTEPPGQGTEGPEGPELETQDGGGPTSGSGGDSPEATGPAGEKSNEEEAAEGAEAVVEPVDAVDPTEPSPAEDLPSLSAEEIAEGLDLLPGTPPPPPPEDEGWEQGENHAGEADGDPLVGLEEPEGAEAEVEKKVDVSMVLDKDQDEEEEELASPEEDGPLALRPSAKKKSRPCSLPVSELETVIASACGEPETPRTHYIRIHNLLHSMPSAQGGSDGGEAQGPEEEDEEGPGEEESTVKEASEKDVVSEAETVAPDPPHPGDGGESGDPEVSGQRPVPHSASAECLAGVNGGPLLDGEPRPSTGSESDSSPRQDCEGCDTSCYSPSCYSTSCYSTSCYSTSCYSTSCYSPSCYDSNNRFSSHTRFSSVDSAKISESTVFSSQDEEEEENSAFESVPDSVQSPEMDRETADGTGPWPDELVLPNGNVPRTADGLESPIAGPSNRREAEVLHFHHIISSAWISLAGDCPLLHNSQPVSQLPSLRPDHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTTAATPDGIRRSGSVQQMEQLNRRYQNIQRTIATERSEDEPPSQSAEPLPAGGGSGGGGGGSDLEADSSQPNLELRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTNSTCLKHMILKIRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRNQHHHETLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHALREKIHYIRTEGTQGLEKLSCDADLVILLSLFEEEIMSYIPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASVRAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_029063052.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029063052.1

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTTAAGAAT CTGTACCAGA ACAGGTTCCT GGGTCTGGCC 
GCCATGGCCT CGCCGTCGAG GAACTCCCAG AGCCGGCGCC GCTGTAAAGA GCCCCTGCGC 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACATCA GGAACGGCCC ACACGGGACC 
GTCACCATCC CCCGCTCCAC CAGCGACACC GACCTGGTCA CGTCCGACAG TCGGTCCACC 
CTTATGGTCA GCAGTTCGTA TTACTCCATT GGACACTCTC AGGACCTGGT GATCTATTGG 
GATATAAAAG AGGAAGTGGG AGCCGGGGAC TGGATTGGCA TGTATCTCAT CGATGAGGTC 
TTATCTGAAA ATTTTCTGGA CTACAAAAAC CGCGGAGTGA ATGGTTCTCA CCGGGGCCAG 
ATTATCTGGA AGATTGATGC AAGTTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTATC ATGGAGTGAG TGGAGCGCTA CGAGCCACTA CGCCCAGTGT TACAGTGAAA 
AACTCTTCAG CTCCTGTTTT TAAGAGCATC ACCAATGACG AGGCCACCCA GGGTCAAGGC 
AGTCGGAGGC TGATCAGTTT TTCTCTCTCA GATTTCCAGG CCATGGGTCT GAAGAAAGGA 
ATGTTCTTTA ACCCAGATCC ATATCTGAAG ATTTCCATCC AACCTGGGAA ACACAGCATC 
TTCCCAGCCC TTCCGCACCA TGGTCAGGAG AAGAGGTCGA AGATTACCTG TAACACCGTG 
AACCCTGTTT GGCAAGCTGA GCAGTTTAGC TTTGTTTCCC TGCCCACCGA CGTCCTAGAA 
ATCGAGGTGA AAGACAAGTT TGCCAAGAGT CGTCCAATCA TCAAACGCTT CTTAGGAAAG 
TTGTCCATGC CTGTTCAGCG ACTCCTGGAG AGACACGCTA TAGGGGACAG GGTGGTGAGC 
TACACTCTGG GTCGAAGGCT TCCAACGGAT CACGTCAGCG GCCAGCTCCA GTTCCGGTTC 
GAGATCACCT CCTCCATCCA CCCAGATGAC GAGGAGGTCT CTCTGAGCGC AGATCCCGAG 
TCTGCTGAAA ACCAGGAGAG TCCTGCCAAC CACCCATCAG AGGAAGGCCT CACAGAACCT 
CCAGGCCAGG GGACGGAGGG CCCAGAGGGC CCGGAGCTGG AGACACAGGA TGGTGGTGGT 
CCCACGAGTG GCTCTGGGGG AGACAGCCCC GAGGCTACCG GACCGGCCGG GGAAAAGTCG 
AATGAGGAGG AGGCCGCAGA GGGTGCCGAA GCCGTCGTGG AGCCAGTTGA TGCCGTCGAT 
CCCACGGAAC CCAGCCCGGC CGAAGATTTG CCTTCCCTCA GTGCTGAAGA GATCGCCGAG 
GGGCTGGATC TGCTCCCTGG CACTCCCCCG CCACCGCCTC CGGAAGACGA GGGTTGGGAG 
CAGGGGGAGA ACCACGCCGG AGAGGCCGAT GGAGATCCCC TTGTCGGCCT GGAGGAGCCA 
GAGGGGGCTG AGGCAGAGGT GGAGAAAAAG GTTGACGTGA GCATGGTTCT AGACAAAGAC 
CAGGATGAAG AGGAAGAAGA ATTGGCCTCC CCCGAAGAGG ATGGGCCTCT GGCGCTGAGA 
CCCTCGGCCA AGAAGAAAAG CAGACCGTGC TCCCTGCCCG TGTCCGAGTT GGAGACGGTG 
ATCGCGTCGG CCTGCGGCGA ACCGGAAACG CCCCGCACCC ATTACATCCG CATCCACAAC 
CTGCTGCACA GCATGCCCTC AGCCCAGGGA GGCAGCGATG GCGGAGAAGC CCAAGGACCG 
GAGGAAGAGG ACGAGGAGGG GCCGGGGGAG GAAGAGTCAA CGGTCAAGGA GGCCTCCGAA 
AAGGACGTGG TCAGCGAGGC CGAGACCGTG GCACCGGACC CACCTCACCC AGGAGATGGC 
GGAGAGAGCG GGGACCCCGA GGTATCTGGC CAGCGGCCGG TGCCCCACAG TGCCTCGGCC 
GAGTGCTTGG CCGGGGTGAA CGGTGGCCCG CTTCTCGACG GCGAGCCCCG GCCCAGCACG 
GGGAGCGAGA GCGACTCGAG CCCCCGGCAG GACTGCGAGG GCTGCGACAC ATCCTGCTAC 
AGCCCGTCCT GCTACAGCAC CTCGTGTTAC AGCACCTCCT GCTACAGCAC CTCGTGCTAC 
AGCACCTCCT GCTACAGCCC ATCCTGCTAC GACAGCAACA ACCGGTTCTC CAGCCATACG 
CGCTTCTCCT CGGTGGACAG TGCCAAGATC TCGGAGAGCA CCGTCTTCTC CTCTCAGGAC 
GAGGAGGAGG AGGAGAACAG CGCGTTCGAG TCGGTGCCCG ACTCGGTGCA GAGCCCGGAG 
ATGGACCGGG AGACGGCCGA CGGCACCGGC CCGTGGCCGG ACGAGCTGGT TCTCCCCAAC 
GGGAACGTTC CGAGGACAGC CGATGGCCTG GAGTCCCCGA TAGCTGGTCC CAGTAATCGG 
AGAGAAGCTG AAGTACTACA TTTTCATCAT ATCATTTCGT CGGCTTGGAT TTCACTAGCA 
GGTGATTGTC CTTTACTACA TAATTCTCAG CCAGTCAGCC AGCTTCCTTC CTTGAGGCCT 
GACCACCACC ATTATCCAAC TATCGATGAG CCTCTTCCGC CAAACTGGGA GGCTCGAATA 
GACAGTCACG GGAGAGTGTT TTACGTGGAC CACGTGAACC GAACGACGAC GTGGCAGCGG 
CCTACCACGG CCGCCACTCC AGACGGGATC CGCAGATCTG GCTCCGTACA GCAGATGGAA 
CAGCTGAACC GCCGGTACCA GAATATCCAG CGGACTATTG CTACCGAGAG ATCGGAAGAC 
GAGCCACCCA GTCAGAGTGC GGAGCCTCTC CCTGCCGGAG GAGGGAGTGG AGGAGGGGGA 
GGAGGGAGTG ACTTGGAAGC AGACTCTTCT CAACCAAATC TAGAACTGAG GAGAGAGGGG 
TCTCTTTCTC CTGTGAACTC CCAAAAAATC ACTTTGCTGC TTCAGTCCCC GGCCGTCAAG 
TTCATCACCA ACCCTGAGTT CTTCACCGTG CTGCATGCCA ACTATAGTGC CTATAGAGTC 
TTCACCAACA GCACCTGCTT GAAGCATATG ATTCTGAAGA TCCGCAGGGA TGCCCGGAAT 
TTCGAACGCT ACCAGCACAA CCGGGACCTG GTGAACTTCA TCAACATGTT TGCTGACACC 
CGTTTGGAAC TGCCTCGCGG CTGGGAAATC AAAACTGACC AGCAGGGCAA GTCCTTCTTC 
GTCGACCACA ACAGTCGAGC CACCACTTTC ATCGACCCCC GGATCCCTCT TCAGAACGGA 
CGTCTTCCGA ATCACCTGAC TCACCGGCAG CACCTTCAGC GACTCCGGAG CTACAGTGCC 
GGCGAGGCCT CAGAAGTCTC TCGGAACCGA GGAGCTTCTC TGTTGGCCAG GCCGGGTCAC 
AGCCTGGTAG CAGCCATCAG AAACCAACAT CACCATGAGA CGTTACCTCT GGCTTATAAT 
GACAAGATCG TGGCATTTCT CCGCCAGCCA AACATTTTTG AGATGCTGCA GGAGCGCCAG 
CCCAGCTTGG CCAGAAACCA CGCACTCAGG GAGAAAATCC ATTACATTCG GACAGAAGGT 
ACTCAAGGGC TTGAAAAATT GTCCTGTGAT GCAGATTTAG TAATACTGCT GAGCCTCTTT 
GAAGAAGAGA TCATGTCCTA CATACCCCTT CAGGCCGCTT TCCACCCGGG ATACAGCTTC 
TCTCCTCGCT GTTCACCGTG CTCTTCCCCT CAAAACTCTC CAGGCCTACA GCGAGCCAGT 
GTCAGGGCCC CCTCACCCTA CAGGAGAGAC TTTGAGGCCA AGCTCCGAAA TTTCTACCGA 
AAACTTGAAG CCAAAGGATT TGGTCAAGGT CCGGGTAAAA TCAAACTCAT CATAAGACGT 
GACCACTTGC TGGAGGGAAC CTTCAATCAG GTGATGGCTT ATTCCCGCAA AGAGCTCCAG 
CGGAACAAGC TCTACGTCAC ATTTGTTGGA GAGGAGGGCC TAGACTACAG TGGGCCCTCC 
CGGGAGTTCT TTTTCCTTTT GTCCCAGGAG CTCTTCAACC CCTACTACGG CCTGTTTGAG 
TATTCTGCCA ATGACACTTA CACCGTGCAG ATCAGCCCCA TGTCGGCCTT TGTGGAAAAT 
CATCTCGAGT GGTTCAGATT TAGCGGTCGC ATCCTTGGGC TTGCCCTGAT CCACCAGTAC 
CTCCTGGACG CTTTCTTCAC GAGGCCGTTC TACAAGGCCC TGCTGAGACT GCCCTGTGAT 
CTGAGCGACC TAGAGTATCT GGATGAGGAA TTCCACCAAA GCCTGCAGTG GATGAAGGAC 
AATAACATCA CGGATATCTT AGATCTCACT TTCACAGTGA ATGAAGAAGT GTTTGGACAG 
GTAACAGAAA GAGAGTTGAA ATCAGGGGGA GCCAACACCC AGGTGACAGA AAAAAATAAA 
AAGGAGTACA TTGAGAGAAT GGTGAAGTGG AGAGTGGAGC GGGGCGTGGT GCAGCAGACG 
GAAGCGTTAG TCCGAGGCTT CTATGAAGTT GTGGATTCAA GGCTCGTCTC CGTCTTTGAT 
GCCAGGGAGC TGGAGCTGGT GATCGCCGGC ACCGCCGAAA TAGACCTCAA TGACTGGCGC 
AATAACACCG AGTATCGAGG GGGTTATCAC GATGGTCATA TCGTGATCCG GTGGTTCTGG 
GCAGCTGTGG AGAGGTTCAA CAATGAGCAG AGACTGAGGT TGCTTCAGTT CGTCACGGGA 
ACATCGAGCG TACCCTATGA AGGTTTCGCC GCGCTCCGAG GAAGCAATGG GCTGAGGCGC 
TTCTGTATAG AGAAATGGGG GAAAATAACA TCCCTTCCCA GGGCACACAC GTGTTTCAAC 
CGCTTGGATC TGCCACCGTA CCCATCGTAT TCCATGCTGT ACGAGAAGCT GTTGACAGCA 
GTTGAGGAAA CGAGCACCTT TGGTCTGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_029063051.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028918884.1 Ornithorhynchus anatinus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.

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CloneID	OOc127714
Clone ID Related Accession (Same CDS sequence)	XM_029063052.1 , XM_029063051.1 , XM_029063053.1
Accession Version	XM_029063051.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	E3 ubiquitin-protein ligase HECW1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041731.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTTAAGAAT CTGTACCAGA ACAGGTTCCT GGGTCTGGCC 
GCCATGGCCT CGCCGTCGAG GAACTCCCAG AGCCGGCGCC GCTGTAAAGA GCCCCTGCGC 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACATCA GGAACGGCCC ACACGGGACC 
GTCACCATCC CCCGCTCCAC CAGCGACACC GACCTGGTCA CGTCCGACAG TCGGTCCACC 
CTTATGGTCA GCAGTTCGTA TTACTCCATT GGACACTCTC AGGACCTGGT GATCTATTGG 
GATATAAAAG AGGAAGTGGG AGCCGGGGAC TGGATTGGCA TGTATCTCAT CGATGAGGTC 
TTATCTGAAA ATTTTCTGGA CTACAAAAAC CGCGGAGTGA ATGGTTCTCA CCGGGGCCAG 
ATTATCTGGA AGATTGATGC AAGTTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTATC ATGGAGTGAG TGGAGCGCTA CGAGCCACTA CGCCCAGTGT TACAGTGAAA 
AACTCTTCAG CTCCTGTTTT TAAGAGCATC ACCAATGACG AGGCCACCCA GGGTCAAGGC 
AGTCGGAGGC TGATCAGTTT TTCTCTCTCA GATTTCCAGG CCATGGGTCT GAAGAAAGGA 
ATGTTCTTTA ACCCAGATCC ATATCTGAAG ATTTCCATCC AACCTGGGAA ACACAGCATC 
TTCCCAGCCC TTCCGCACCA TGGTCAGGAG AAGAGGTCGA AGATTACCTG TAACACCGTG 
AACCCTGTTT GGCAAGCTGA GCAGTTTAGC TTTGTTTCCC TGCCCACCGA CGTCCTAGAA 
ATCGAGGTGA AAGACAAGTT TGCCAAGAGT CGTCCAATCA TCAAACGCTT CTTAGGAAAG 
TTGTCCATGC CTGTTCAGCG ACTCCTGGAG AGACACGCTA TAGGGGACAG GGTGGTGAGC 
TACACTCTGG GTCGAAGGCT TCCAACGGAT CACGTCAGCG GCCAGCTCCA GTTCCGGTTC 
GAGATCACCT CCTCCATCCA CCCAGATGAC GAGGAGGTCT CTCTGAGCGC AGATCCCGAG 
TCTGCTGAAA ACCAGGAGAG TCCTGCCAAC CACCCATCAG AGGAAGGCCT CACAGAACCT 
CCAGGCCAGG GGACGGAGGG CCCAGAGGGC CCGGAGCTGG AGACACAGGA TGGTGGTGGT 
CCCACGAGTG GCTCTGGGGG AGACAGCCCC GAGGCTACCG GACCGGCCGG GGAAAAGTCG 
AATGAGGAGG AGGCCGCAGA GGGTGCCGAA GCCGTCGTGG AGCCAGTTGA TGCCGTCGAT 
CCCACGGAAC CCAGCCCGGC CGAAGATTTG CCTTCCCTCA GTGCTGAAGA GATCGCCGAG 
GGGCTGGATC TGCTCCCTGG CACTCCCCCG CCACCGCCTC CGGAAGACGA GGGTTGGGAG 
CAGGGGGAGA ACCACGCCGG AGAGGCCGAT GGAGATCCCC TTGTCGGCCT GGAGGAGCCA 
GAGGGGGCTG AGGCAGAGGT GGAGAAAAAG GTTGACGTGA GCATGGTTCT AGACAAAGAC 
CAGGATGAAG AGGAAGAAGA ATTGGCCTCC CCCGAAGAGG ATGGGCCTCT GGCGCTGAGA 
CCCTCGGCCA AGAAGAAAAG CAGACCGTGC TCCCTGCCCG TGTCCGAGTT GGAGACGGTG 
ATCGCGTCGG CCTGCGGCGA ACCGGAAACG CCCCGCACCC ATTACATCCG CATCCACAAC 
CTGCTGCACA GCATGCCCTC AGCCCAGGGA GGCAGCGATG GCGGAGAAGC CCAAGGACCG 
GAGGAAGAGG ACGAGGAGGG GCCGGGGGAG GAAGAGTCAA CGGTCAAGGA GGCCTCCGAA 
AAGGACGTGG TCAGCGAGGC CGAGACCGTG GCACCGGACC CACCTCACCC AGGAGATGGC 
GGAGAGAGCG GGGACCCCGA GGTATCTGGC CAGCGGCCGG TGCCCCACAG TGCCTCGGCC 
GAGTGCTTGG CCGGGGTGAA CGGTGGCCCG CTTCTCGACG GCGAGCCCCG GCCCAGCACG 
GGGAGCGAGA GCGACTCGAG CCCCCGGCAG GACTGCGAGG GCTGCGACAC ATCCTGCTAC 
AGCCCGTCCT GCTACAGCAC CTCGTGTTAC AGCACCTCCT GCTACAGCAC CTCGTGCTAC 
AGCACCTCCT GCTACAGCCC ATCCTGCTAC GACAGCAACA ACCGGTTCTC CAGCCATACG 
CGCTTCTCCT CGGTGGACAG TGCCAAGATC TCGGAGAGCA CCGTCTTCTC CTCTCAGGAC 
GAGGAGGAGG AGGAGAACAG CGCGTTCGAG TCGGTGCCCG ACTCGGTGCA GAGCCCGGAG 
ATGGACCGGG AGACGGCCGA CGGCACCGGC CCGTGGCCGG ACGAGCTGGT TCTCCCCAAC 
GGGAACGTTC CGAGGACAGC CGATGGCCTG GAGTCCCCGA TAGCTGGTCC CAGTAATCGG 
AGAGAAGCTG AAGTACTACA TTTTCATCAT ATCATTTCGT CGGCTTGGAT TTCACTAGCA 
GGTGATTGTC CTTTACTACA TAATTCTCAG CCAGTCAGCC AGCTTCCTTC CTTGAGGCCT 
GACCACCACC ATTATCCAAC TATCGATGAG CCTCTTCCGC CAAACTGGGA GGCTCGAATA 
GACAGTCACG GGAGAGTGTT TTACGTGGAC CACGTGAACC GAACGACGAC GTGGCAGCGG 
CCTACCACGG CCGCCACTCC AGACGGGATC CGCAGATCTG GCTCCGTACA GCAGATGGAA 
CAGCTGAACC GCCGGTACCA GAATATCCAG CGGACTATTG CTACCGAGAG ATCGGAAGAC 
GAGCCACCCA GTCAGAGTGC GGAGCCTCTC CCTGCCGGAG GAGGGAGTGG AGGAGGGGGA 
GGAGGGAGTG ACTTGGAAGC AGACTCTTCT CAACCAAATC TAGAACTGAG GAGAGAGGGG 
TCTCTTTCTC CTGTGAACTC CCAAAAAATC ACTTTGCTGC TTCAGTCCCC GGCCGTCAAG 
TTCATCACCA ACCCTGAGTT CTTCACCGTG CTGCATGCCA ACTATAGTGC CTATAGAGTC 
TTCACCAACA GCACCTGCTT GAAGCATATG ATTCTGAAGA TCCGCAGGGA TGCCCGGAAT 
TTCGAACGCT ACCAGCACAA CCGGGACCTG GTGAACTTCA TCAACATGTT TGCTGACACC 
CGTTTGGAAC TGCCTCGCGG CTGGGAAATC AAAACTGACC AGCAGGGCAA GTCCTTCTTC 
GTCGACCACA ACAGTCGAGC CACCACTTTC ATCGACCCCC GGATCCCTCT TCAGAACGGA 
CGTCTTCCGA ATCACCTGAC TCACCGGCAG CACCTTCAGC GACTCCGGAG CTACAGTGCC 
GGCGAGGCCT CAGAAGTCTC TCGGAACCGA GGAGCTTCTC TGTTGGCCAG GCCGGGTCAC 
AGCCTGGTAG CAGCCATCAG AAACCAACAT CACCATGAGA CGTTACCTCT GGCTTATAAT 
GACAAGATCG TGGCATTTCT CCGCCAGCCA AACATTTTTG AGATGCTGCA GGAGCGCCAG 
CCCAGCTTGG CCAGAAACCA CGCACTCAGG GAGAAAATCC ATTACATTCG GACAGAAGGT 
ACTCAAGGGC TTGAAAAATT GTCCTGTGAT GCAGATTTAG TAATACTGCT GAGCCTCTTT 
GAAGAAGAGA TCATGTCCTA CATACCCCTT CAGGCCGCTT TCCACCCGGG ATACAGCTTC 
TCTCCTCGCT GTTCACCGTG CTCTTCCCCT CAAAACTCTC CAGGCCTACA GCGAGCCAGT 
GTCAGGGCCC CCTCACCCTA CAGGAGAGAC TTTGAGGCCA AGCTCCGAAA TTTCTACCGA 
AAACTTGAAG CCAAAGGATT TGGTCAAGGT CCGGGTAAAA TCAAACTCAT CATAAGACGT 
GACCACTTGC TGGAGGGAAC CTTCAATCAG GTGATGGCTT ATTCCCGCAA AGAGCTCCAG 
CGGAACAAGC TCTACGTCAC ATTTGTTGGA GAGGAGGGCC TAGACTACAG TGGGCCCTCC 
CGGGAGTTCT TTTTCCTTTT GTCCCAGGAG CTCTTCAACC CCTACTACGG CCTGTTTGAG 
TATTCTGCCA ATGACACTTA CACCGTGCAG ATCAGCCCCA TGTCGGCCTT TGTGGAAAAT 
CATCTCGAGT GGTTCAGATT TAGCGGTCGC ATCCTTGGGC TTGCCCTGAT CCACCAGTAC 
CTCCTGGACG CTTTCTTCAC GAGGCCGTTC TACAAGGCCC TGCTGAGACT GCCCTGTGAT 
CTGAGCGACC TAGAGTATCT GGATGAGGAA TTCCACCAAA GCCTGCAGTG GATGAAGGAC 
AATAACATCA CGGATATCTT AGATCTCACT TTCACAGTGA ATGAAGAAGT GTTTGGACAG 
GTAACAGAAA GAGAGTTGAA ATCAGGGGGA GCCAACACCC AGGTGACAGA AAAAAATAAA 
AAGGAGTACA TTGAGAGAAT GGTGAAGTGG AGAGTGGAGC GGGGCGTGGT GCAGCAGACG 
GAAGCGTTAG TCCGAGGCTT CTATGAAGTT GTGGATTCAA GGCTCGTCTC CGTCTTTGAT 
GCCAGGGAGC TGGAGCTGGT GATCGCCGGC ACCGCCGAAA TAGACCTCAA TGACTGGCGC 
AATAACACCG AGTATCGAGG GGGTTATCAC GATGGTCATA TCGTGATCCG GTGGTTCTGG 
GCAGCTGTGG AGAGGTTCAA CAATGAGCAG AGACTGAGGT TGCTTCAGTT CGTCACGGGA 
ACATCGAGCG TACCCTATGA AGGTTTCGCC GCGCTCCGAG GAAGCAATGG GCTGAGGCGC 
TTCTGTATAG AGAAATGGGG GAAAATAACA TCCCTTCCCA GGGCACACAC GTGTTTCAAC 
CGCTTGGATC TGCCACCGTA CCCATCGTAT TCCATGCTGT ACGAGAAGCT GTTGACAGCA 
GTTGAGGAAA CGAGCACCTT TGGTCTGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028918884.1
CDS	293..5245
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDIRNGPHGTVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIYWDIKEEVGAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSSAPVFKSITNDEATQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQEKRSKITCNTVNPVWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEVSLSADPESAENQESPANHPSEEGLTEPPGQGTEGPEGPELETQDGGGPTSGSGGDSPEATGPAGEKSNEEEAAEGAEAVVEPVDAVDPTEPSPAEDLPSLSAEEIAEGLDLLPGTPPPPPPEDEGWEQGENHAGEADGDPLVGLEEPEGAEAEVEKKVDVSMVLDKDQDEEEEELASPEEDGPLALRPSAKKKSRPCSLPVSELETVIASACGEPETPRTHYIRIHNLLHSMPSAQGGSDGGEAQGPEEEDEEGPGEEESTVKEASEKDVVSEAETVAPDPPHPGDGGESGDPEVSGQRPVPHSASAECLAGVNGGPLLDGEPRPSTGSESDSSPRQDCEGCDTSCYSPSCYSTSCYSTSCYSTSCYSTSCYSPSCYDSNNRFSSHTRFSSVDSAKISESTVFSSQDEEEEENSAFESVPDSVQSPEMDRETADGTGPWPDELVLPNGNVPRTADGLESPIAGPSNRREAEVLHFHHIISSAWISLAGDCPLLHNSQPVSQLPSLRPDHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTTAATPDGIRRSGSVQQMEQLNRRYQNIQRTIATERSEDEPPSQSAEPLPAGGGSGGGGGGSDLEADSSQPNLELRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTNSTCLKHMILKIRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRNQHHHETLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHALREKIHYIRTEGTQGLEKLSCDADLVILLSLFEEEIMSYIPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASVRAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_029063051.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029063051.1

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTTAAGAAT CTGTACCAGA ACAGGTTCCT GGGTCTGGCC 
GCCATGGCCT CGCCGTCGAG GAACTCCCAG AGCCGGCGCC GCTGTAAAGA GCCCCTGCGC 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACATCA GGAACGGCCC ACACGGGACC 
GTCACCATCC CCCGCTCCAC CAGCGACACC GACCTGGTCA CGTCCGACAG TCGGTCCACC 
CTTATGGTCA GCAGTTCGTA TTACTCCATT GGACACTCTC AGGACCTGGT GATCTATTGG 
GATATAAAAG AGGAAGTGGG AGCCGGGGAC TGGATTGGCA TGTATCTCAT CGATGAGGTC 
TTATCTGAAA ATTTTCTGGA CTACAAAAAC CGCGGAGTGA ATGGTTCTCA CCGGGGCCAG 
ATTATCTGGA AGATTGATGC AAGTTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTATC ATGGAGTGAG TGGAGCGCTA CGAGCCACTA CGCCCAGTGT TACAGTGAAA 
AACTCTTCAG CTCCTGTTTT TAAGAGCATC ACCAATGACG AGGCCACCCA GGGTCAAGGC 
AGTCGGAGGC TGATCAGTTT TTCTCTCTCA GATTTCCAGG CCATGGGTCT GAAGAAAGGA 
ATGTTCTTTA ACCCAGATCC ATATCTGAAG ATTTCCATCC AACCTGGGAA ACACAGCATC 
TTCCCAGCCC TTCCGCACCA TGGTCAGGAG AAGAGGTCGA AGATTACCTG TAACACCGTG 
AACCCTGTTT GGCAAGCTGA GCAGTTTAGC TTTGTTTCCC TGCCCACCGA CGTCCTAGAA 
ATCGAGGTGA AAGACAAGTT TGCCAAGAGT CGTCCAATCA TCAAACGCTT CTTAGGAAAG 
TTGTCCATGC CTGTTCAGCG ACTCCTGGAG AGACACGCTA TAGGGGACAG GGTGGTGAGC 
TACACTCTGG GTCGAAGGCT TCCAACGGAT CACGTCAGCG GCCAGCTCCA GTTCCGGTTC 
GAGATCACCT CCTCCATCCA CCCAGATGAC GAGGAGGTCT CTCTGAGCGC AGATCCCGAG 
TCTGCTGAAA ACCAGGAGAG TCCTGCCAAC CACCCATCAG AGGAAGGCCT CACAGAACCT 
CCAGGCCAGG GGACGGAGGG CCCAGAGGGC CCGGAGCTGG AGACACAGGA TGGTGGTGGT 
CCCACGAGTG GCTCTGGGGG AGACAGCCCC GAGGCTACCG GACCGGCCGG GGAAAAGTCG 
AATGAGGAGG AGGCCGCAGA GGGTGCCGAA GCCGTCGTGG AGCCAGTTGA TGCCGTCGAT 
CCCACGGAAC CCAGCCCGGC CGAAGATTTG CCTTCCCTCA GTGCTGAAGA GATCGCCGAG 
GGGCTGGATC TGCTCCCTGG CACTCCCCCG CCACCGCCTC CGGAAGACGA GGGTTGGGAG 
CAGGGGGAGA ACCACGCCGG AGAGGCCGAT GGAGATCCCC TTGTCGGCCT GGAGGAGCCA 
GAGGGGGCTG AGGCAGAGGT GGAGAAAAAG GTTGACGTGA GCATGGTTCT AGACAAAGAC 
CAGGATGAAG AGGAAGAAGA ATTGGCCTCC CCCGAAGAGG ATGGGCCTCT GGCGCTGAGA 
CCCTCGGCCA AGAAGAAAAG CAGACCGTGC TCCCTGCCCG TGTCCGAGTT GGAGACGGTG 
ATCGCGTCGG CCTGCGGCGA ACCGGAAACG CCCCGCACCC ATTACATCCG CATCCACAAC 
CTGCTGCACA GCATGCCCTC AGCCCAGGGA GGCAGCGATG GCGGAGAAGC CCAAGGACCG 
GAGGAAGAGG ACGAGGAGGG GCCGGGGGAG GAAGAGTCAA CGGTCAAGGA GGCCTCCGAA 
AAGGACGTGG TCAGCGAGGC CGAGACCGTG GCACCGGACC CACCTCACCC AGGAGATGGC 
GGAGAGAGCG GGGACCCCGA GGTATCTGGC CAGCGGCCGG TGCCCCACAG TGCCTCGGCC 
GAGTGCTTGG CCGGGGTGAA CGGTGGCCCG CTTCTCGACG GCGAGCCCCG GCCCAGCACG 
GGGAGCGAGA GCGACTCGAG CCCCCGGCAG GACTGCGAGG GCTGCGACAC ATCCTGCTAC 
AGCCCGTCCT GCTACAGCAC CTCGTGTTAC AGCACCTCCT GCTACAGCAC CTCGTGCTAC 
AGCACCTCCT GCTACAGCCC ATCCTGCTAC GACAGCAACA ACCGGTTCTC CAGCCATACG 
CGCTTCTCCT CGGTGGACAG TGCCAAGATC TCGGAGAGCA CCGTCTTCTC CTCTCAGGAC 
GAGGAGGAGG AGGAGAACAG CGCGTTCGAG TCGGTGCCCG ACTCGGTGCA GAGCCCGGAG 
ATGGACCGGG AGACGGCCGA CGGCACCGGC CCGTGGCCGG ACGAGCTGGT TCTCCCCAAC 
GGGAACGTTC CGAGGACAGC CGATGGCCTG GAGTCCCCGA TAGCTGGTCC CAGTAATCGG 
AGAGAAGCTG AAGTACTACA TTTTCATCAT ATCATTTCGT CGGCTTGGAT TTCACTAGCA 
GGTGATTGTC CTTTACTACA TAATTCTCAG CCAGTCAGCC AGCTTCCTTC CTTGAGGCCT 
GACCACCACC ATTATCCAAC TATCGATGAG CCTCTTCCGC CAAACTGGGA GGCTCGAATA 
GACAGTCACG GGAGAGTGTT TTACGTGGAC CACGTGAACC GAACGACGAC GTGGCAGCGG 
CCTACCACGG CCGCCACTCC AGACGGGATC CGCAGATCTG GCTCCGTACA GCAGATGGAA 
CAGCTGAACC GCCGGTACCA GAATATCCAG CGGACTATTG CTACCGAGAG ATCGGAAGAC 
GAGCCACCCA GTCAGAGTGC GGAGCCTCTC CCTGCCGGAG GAGGGAGTGG AGGAGGGGGA 
GGAGGGAGTG ACTTGGAAGC AGACTCTTCT CAACCAAATC TAGAACTGAG GAGAGAGGGG 
TCTCTTTCTC CTGTGAACTC CCAAAAAATC ACTTTGCTGC TTCAGTCCCC GGCCGTCAAG 
TTCATCACCA ACCCTGAGTT CTTCACCGTG CTGCATGCCA ACTATAGTGC CTATAGAGTC 
TTCACCAACA GCACCTGCTT GAAGCATATG ATTCTGAAGA TCCGCAGGGA TGCCCGGAAT 
TTCGAACGCT ACCAGCACAA CCGGGACCTG GTGAACTTCA TCAACATGTT TGCTGACACC 
CGTTTGGAAC TGCCTCGCGG CTGGGAAATC AAAACTGACC AGCAGGGCAA GTCCTTCTTC 
GTCGACCACA ACAGTCGAGC CACCACTTTC ATCGACCCCC GGATCCCTCT TCAGAACGGA 
CGTCTTCCGA ATCACCTGAC TCACCGGCAG CACCTTCAGC GACTCCGGAG CTACAGTGCC 
GGCGAGGCCT CAGAAGTCTC TCGGAACCGA GGAGCTTCTC TGTTGGCCAG GCCGGGTCAC 
AGCCTGGTAG CAGCCATCAG AAACCAACAT CACCATGAGA CGTTACCTCT GGCTTATAAT 
GACAAGATCG TGGCATTTCT CCGCCAGCCA AACATTTTTG AGATGCTGCA GGAGCGCCAG 
CCCAGCTTGG CCAGAAACCA CGCACTCAGG GAGAAAATCC ATTACATTCG GACAGAAGGT 
ACTCAAGGGC TTGAAAAATT GTCCTGTGAT GCAGATTTAG TAATACTGCT GAGCCTCTTT 
GAAGAAGAGA TCATGTCCTA CATACCCCTT CAGGCCGCTT TCCACCCGGG ATACAGCTTC 
TCTCCTCGCT GTTCACCGTG CTCTTCCCCT CAAAACTCTC CAGGCCTACA GCGAGCCAGT 
GTCAGGGCCC CCTCACCCTA CAGGAGAGAC TTTGAGGCCA AGCTCCGAAA TTTCTACCGA 
AAACTTGAAG CCAAAGGATT TGGTCAAGGT CCGGGTAAAA TCAAACTCAT CATAAGACGT 
GACCACTTGC TGGAGGGAAC CTTCAATCAG GTGATGGCTT ATTCCCGCAA AGAGCTCCAG 
CGGAACAAGC TCTACGTCAC ATTTGTTGGA GAGGAGGGCC TAGACTACAG TGGGCCCTCC 
CGGGAGTTCT TTTTCCTTTT GTCCCAGGAG CTCTTCAACC CCTACTACGG CCTGTTTGAG 
TATTCTGCCA ATGACACTTA CACCGTGCAG ATCAGCCCCA TGTCGGCCTT TGTGGAAAAT 
CATCTCGAGT GGTTCAGATT TAGCGGTCGC ATCCTTGGGC TTGCCCTGAT CCACCAGTAC 
CTCCTGGACG CTTTCTTCAC GAGGCCGTTC TACAAGGCCC TGCTGAGACT GCCCTGTGAT 
CTGAGCGACC TAGAGTATCT GGATGAGGAA TTCCACCAAA GCCTGCAGTG GATGAAGGAC 
AATAACATCA CGGATATCTT AGATCTCACT TTCACAGTGA ATGAAGAAGT GTTTGGACAG 
GTAACAGAAA GAGAGTTGAA ATCAGGGGGA GCCAACACCC AGGTGACAGA AAAAAATAAA 
AAGGAGTACA TTGAGAGAAT GGTGAAGTGG AGAGTGGAGC GGGGCGTGGT GCAGCAGACG 
GAAGCGTTAG TCCGAGGCTT CTATGAAGTT GTGGATTCAA GGCTCGTCTC CGTCTTTGAT 
GCCAGGGAGC TGGAGCTGGT GATCGCCGGC ACCGCCGAAA TAGACCTCAA TGACTGGCGC 
AATAACACCG AGTATCGAGG GGGTTATCAC GATGGTCATA TCGTGATCCG GTGGTTCTGG 
GCAGCTGTGG AGAGGTTCAA CAATGAGCAG AGACTGAGGT TGCTTCAGTT CGTCACGGGA 
ACATCGAGCG TACCCTATGA AGGTTTCGCC GCGCTCCGAG GAAGCAATGG GCTGAGGCGC 
TTCTGTATAG AGAAATGGGG GAAAATAACA TCCCTTCCCA GGGCACACAC GTGTTTCAAC 
CGCTTGGATC TGCCACCGTA CCCATCGTAT TCCATGCTGT ACGAGAAGCT GTTGACAGCA 
GTTGAGGAAA CGAGCACCTT TGGTCTGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_029063053.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028918886.1 Ornithorhynchus anatinus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.

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CloneID	OOc127714
Clone ID Related Accession (Same CDS sequence)	XM_029063052.1 , XM_029063051.1 , XM_029063053.1
Accession Version	XM_029063053.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	E3 ubiquitin-protein ligase HECW1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041731.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTTAAGAAT CTGTACCAGA ACAGGTTCCT GGGTCTGGCC 
GCCATGGCCT CGCCGTCGAG GAACTCCCAG AGCCGGCGCC GCTGTAAAGA GCCCCTGCGC 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACATCA GGAACGGCCC ACACGGGACC 
GTCACCATCC CCCGCTCCAC CAGCGACACC GACCTGGTCA CGTCCGACAG TCGGTCCACC 
CTTATGGTCA GCAGTTCGTA TTACTCCATT GGACACTCTC AGGACCTGGT GATCTATTGG 
GATATAAAAG AGGAAGTGGG AGCCGGGGAC TGGATTGGCA TGTATCTCAT CGATGAGGTC 
TTATCTGAAA ATTTTCTGGA CTACAAAAAC CGCGGAGTGA ATGGTTCTCA CCGGGGCCAG 
ATTATCTGGA AGATTGATGC AAGTTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTATC ATGGAGTGAG TGGAGCGCTA CGAGCCACTA CGCCCAGTGT TACAGTGAAA 
AACTCTTCAG CTCCTGTTTT TAAGAGCATC ACCAATGACG AGGCCACCCA GGGTCAAGGC 
AGTCGGAGGC TGATCAGTTT TTCTCTCTCA GATTTCCAGG CCATGGGTCT GAAGAAAGGA 
ATGTTCTTTA ACCCAGATCC ATATCTGAAG ATTTCCATCC AACCTGGGAA ACACAGCATC 
TTCCCAGCCC TTCCGCACCA TGGTCAGGAG AAGAGGTCGA AGATTACCTG TAACACCGTG 
AACCCTGTTT GGCAAGCTGA GCAGTTTAGC TTTGTTTCCC TGCCCACCGA CGTCCTAGAA 
ATCGAGGTGA AAGACAAGTT TGCCAAGAGT CGTCCAATCA TCAAACGCTT CTTAGGAAAG 
TTGTCCATGC CTGTTCAGCG ACTCCTGGAG AGACACGCTA TAGGGGACAG GGTGGTGAGC 
TACACTCTGG GTCGAAGGCT TCCAACGGAT CACGTCAGCG GCCAGCTCCA GTTCCGGTTC 
GAGATCACCT CCTCCATCCA CCCAGATGAC GAGGAGGTCT CTCTGAGCGC AGATCCCGAG 
TCTGCTGAAA ACCAGGAGAG TCCTGCCAAC CACCCATCAG AGGAAGGCCT CACAGAACCT 
CCAGGCCAGG GGACGGAGGG CCCAGAGGGC CCGGAGCTGG AGACACAGGA TGGTGGTGGT 
CCCACGAGTG GCTCTGGGGG AGACAGCCCC GAGGCTACCG GACCGGCCGG GGAAAAGTCG 
AATGAGGAGG AGGCCGCAGA GGGTGCCGAA GCCGTCGTGG AGCCAGTTGA TGCCGTCGAT 
CCCACGGAAC CCAGCCCGGC CGAAGATTTG CCTTCCCTCA GTGCTGAAGA GATCGCCGAG 
GGGCTGGATC TGCTCCCTGG CACTCCCCCG CCACCGCCTC CGGAAGACGA GGGTTGGGAG 
CAGGGGGAGA ACCACGCCGG AGAGGCCGAT GGAGATCCCC TTGTCGGCCT GGAGGAGCCA 
GAGGGGGCTG AGGCAGAGGT GGAGAAAAAG GTTGACGTGA GCATGGTTCT AGACAAAGAC 
CAGGATGAAG AGGAAGAAGA ATTGGCCTCC CCCGAAGAGG ATGGGCCTCT GGCGCTGAGA 
CCCTCGGCCA AGAAGAAAAG CAGACCGTGC TCCCTGCCCG TGTCCGAGTT GGAGACGGTG 
ATCGCGTCGG CCTGCGGCGA ACCGGAAACG CCCCGCACCC ATTACATCCG CATCCACAAC 
CTGCTGCACA GCATGCCCTC AGCCCAGGGA GGCAGCGATG GCGGAGAAGC CCAAGGACCG 
GAGGAAGAGG ACGAGGAGGG GCCGGGGGAG GAAGAGTCAA CGGTCAAGGA GGCCTCCGAA 
AAGGACGTGG TCAGCGAGGC CGAGACCGTG GCACCGGACC CACCTCACCC AGGAGATGGC 
GGAGAGAGCG GGGACCCCGA GGTATCTGGC CAGCGGCCGG TGCCCCACAG TGCCTCGGCC 
GAGTGCTTGG CCGGGGTGAA CGGTGGCCCG CTTCTCGACG GCGAGCCCCG GCCCAGCACG 
GGGAGCGAGA GCGACTCGAG CCCCCGGCAG GACTGCGAGG GCTGCGACAC ATCCTGCTAC 
AGCCCGTCCT GCTACAGCAC CTCGTGTTAC AGCACCTCCT GCTACAGCAC CTCGTGCTAC 
AGCACCTCCT GCTACAGCCC ATCCTGCTAC GACAGCAACA ACCGGTTCTC CAGCCATACG 
CGCTTCTCCT CGGTGGACAG TGCCAAGATC TCGGAGAGCA CCGTCTTCTC CTCTCAGGAC 
GAGGAGGAGG AGGAGAACAG CGCGTTCGAG TCGGTGCCCG ACTCGGTGCA GAGCCCGGAG 
ATGGACCGGG AGACGGCCGA CGGCACCGGC CCGTGGCCGG ACGAGCTGGT TCTCCCCAAC 
GGGAACGTTC CGAGGACAGC CGATGGCCTG GAGTCCCCGA TAGCTGGTCC CAGTAATCGG 
AGAGAAGCTG AAGTACTACA TTTTCATCAT ATCATTTCGT CGGCTTGGAT TTCACTAGCA 
GGTGATTGTC CTTTACTACA TAATTCTCAG CCAGTCAGCC AGCTTCCTTC CTTGAGGCCT 
GACCACCACC ATTATCCAAC TATCGATGAG CCTCTTCCGC CAAACTGGGA GGCTCGAATA 
GACAGTCACG GGAGAGTGTT TTACGTGGAC CACGTGAACC GAACGACGAC GTGGCAGCGG 
CCTACCACGG CCGCCACTCC AGACGGGATC CGCAGATCTG GCTCCGTACA GCAGATGGAA 
CAGCTGAACC GCCGGTACCA GAATATCCAG CGGACTATTG CTACCGAGAG ATCGGAAGAC 
GAGCCACCCA GTCAGAGTGC GGAGCCTCTC CCTGCCGGAG GAGGGAGTGG AGGAGGGGGA 
GGAGGGAGTG ACTTGGAAGC AGACTCTTCT CAACCAAATC TAGAACTGAG GAGAGAGGGG 
TCTCTTTCTC CTGTGAACTC CCAAAAAATC ACTTTGCTGC TTCAGTCCCC GGCCGTCAAG 
TTCATCACCA ACCCTGAGTT CTTCACCGTG CTGCATGCCA ACTATAGTGC CTATAGAGTC 
TTCACCAACA GCACCTGCTT GAAGCATATG ATTCTGAAGA TCCGCAGGGA TGCCCGGAAT 
TTCGAACGCT ACCAGCACAA CCGGGACCTG GTGAACTTCA TCAACATGTT TGCTGACACC 
CGTTTGGAAC TGCCTCGCGG CTGGGAAATC AAAACTGACC AGCAGGGCAA GTCCTTCTTC 
GTCGACCACA ACAGTCGAGC CACCACTTTC ATCGACCCCC GGATCCCTCT TCAGAACGGA 
CGTCTTCCGA ATCACCTGAC TCACCGGCAG CACCTTCAGC GACTCCGGAG CTACAGTGCC 
GGCGAGGCCT CAGAAGTCTC TCGGAACCGA GGAGCTTCTC TGTTGGCCAG GCCGGGTCAC 
AGCCTGGTAG CAGCCATCAG AAACCAACAT CACCATGAGA CGTTACCTCT GGCTTATAAT 
GACAAGATCG TGGCATTTCT CCGCCAGCCA AACATTTTTG AGATGCTGCA GGAGCGCCAG 
CCCAGCTTGG CCAGAAACCA CGCACTCAGG GAGAAAATCC ATTACATTCG GACAGAAGGT 
ACTCAAGGGC TTGAAAAATT GTCCTGTGAT GCAGATTTAG TAATACTGCT GAGCCTCTTT 
GAAGAAGAGA TCATGTCCTA CATACCCCTT CAGGCCGCTT TCCACCCGGG ATACAGCTTC 
TCTCCTCGCT GTTCACCGTG CTCTTCCCCT CAAAACTCTC CAGGCCTACA GCGAGCCAGT 
GTCAGGGCCC CCTCACCCTA CAGGAGAGAC TTTGAGGCCA AGCTCCGAAA TTTCTACCGA 
AAACTTGAAG CCAAAGGATT TGGTCAAGGT CCGGGTAAAA TCAAACTCAT CATAAGACGT 
GACCACTTGC TGGAGGGAAC CTTCAATCAG GTGATGGCTT ATTCCCGCAA AGAGCTCCAG 
CGGAACAAGC TCTACGTCAC ATTTGTTGGA GAGGAGGGCC TAGACTACAG TGGGCCCTCC 
CGGGAGTTCT TTTTCCTTTT GTCCCAGGAG CTCTTCAACC CCTACTACGG CCTGTTTGAG 
TATTCTGCCA ATGACACTTA CACCGTGCAG ATCAGCCCCA TGTCGGCCTT TGTGGAAAAT 
CATCTCGAGT GGTTCAGATT TAGCGGTCGC ATCCTTGGGC TTGCCCTGAT CCACCAGTAC 
CTCCTGGACG CTTTCTTCAC GAGGCCGTTC TACAAGGCCC TGCTGAGACT GCCCTGTGAT 
CTGAGCGACC TAGAGTATCT GGATGAGGAA TTCCACCAAA GCCTGCAGTG GATGAAGGAC 
AATAACATCA CGGATATCTT AGATCTCACT TTCACAGTGA ATGAAGAAGT GTTTGGACAG 
GTAACAGAAA GAGAGTTGAA ATCAGGGGGA GCCAACACCC AGGTGACAGA AAAAAATAAA 
AAGGAGTACA TTGAGAGAAT GGTGAAGTGG AGAGTGGAGC GGGGCGTGGT GCAGCAGACG 
GAAGCGTTAG TCCGAGGCTT CTATGAAGTT GTGGATTCAA GGCTCGTCTC CGTCTTTGAT 
GCCAGGGAGC TGGAGCTGGT GATCGCCGGC ACCGCCGAAA TAGACCTCAA TGACTGGCGC 
AATAACACCG AGTATCGAGG GGGTTATCAC GATGGTCATA TCGTGATCCG GTGGTTCTGG 
GCAGCTGTGG AGAGGTTCAA CAATGAGCAG AGACTGAGGT TGCTTCAGTT CGTCACGGGA 
ACATCGAGCG TACCCTATGA AGGTTTCGCC GCGCTCCGAG GAAGCAATGG GCTGAGGCGC 
TTCTGTATAG AGAAATGGGG GAAAATAACA TCCCTTCCCA GGGCACACAC GTGTTTCAAC 
CGCTTGGATC TGCCACCGTA CCCATCGTAT TCCATGCTGT ACGAGAAGCT GTTGACAGCA 
GTTGAGGAAA CGAGCACCTT TGGTCTGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028918886.1
CDS	164..5116
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDIRNGPHGTVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIYWDIKEEVGAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSSAPVFKSITNDEATQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQEKRSKITCNTVNPVWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEVSLSADPESAENQESPANHPSEEGLTEPPGQGTEGPEGPELETQDGGGPTSGSGGDSPEATGPAGEKSNEEEAAEGAEAVVEPVDAVDPTEPSPAEDLPSLSAEEIAEGLDLLPGTPPPPPPEDEGWEQGENHAGEADGDPLVGLEEPEGAEAEVEKKVDVSMVLDKDQDEEEEELASPEEDGPLALRPSAKKKSRPCSLPVSELETVIASACGEPETPRTHYIRIHNLLHSMPSAQGGSDGGEAQGPEEEDEEGPGEEESTVKEASEKDVVSEAETVAPDPPHPGDGGESGDPEVSGQRPVPHSASAECLAGVNGGPLLDGEPRPSTGSESDSSPRQDCEGCDTSCYSPSCYSTSCYSTSCYSTSCYSTSCYSPSCYDSNNRFSSHTRFSSVDSAKISESTVFSSQDEEEEENSAFESVPDSVQSPEMDRETADGTGPWPDELVLPNGNVPRTADGLESPIAGPSNRREAEVLHFHHIISSAWISLAGDCPLLHNSQPVSQLPSLRPDHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTTAATPDGIRRSGSVQQMEQLNRRYQNIQRTIATERSEDEPPSQSAEPLPAGGGSGGGGGGSDLEADSSQPNLELRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTNSTCLKHMILKIRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRNQHHHETLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHALREKIHYIRTEGTQGLEKLSCDADLVILLSLFEEEIMSYIPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASVRAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_029063053.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029063053.1

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTTAAGAAT CTGTACCAGA ACAGGTTCCT GGGTCTGGCC 
GCCATGGCCT CGCCGTCGAG GAACTCCCAG AGCCGGCGCC GCTGTAAAGA GCCCCTGCGC 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACATCA GGAACGGCCC ACACGGGACC 
GTCACCATCC CCCGCTCCAC CAGCGACACC GACCTGGTCA CGTCCGACAG TCGGTCCACC 
CTTATGGTCA GCAGTTCGTA TTACTCCATT GGACACTCTC AGGACCTGGT GATCTATTGG 
GATATAAAAG AGGAAGTGGG AGCCGGGGAC TGGATTGGCA TGTATCTCAT CGATGAGGTC 
TTATCTGAAA ATTTTCTGGA CTACAAAAAC CGCGGAGTGA ATGGTTCTCA CCGGGGCCAG 
ATTATCTGGA AGATTGATGC AAGTTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTATC ATGGAGTGAG TGGAGCGCTA CGAGCCACTA CGCCCAGTGT TACAGTGAAA 
AACTCTTCAG CTCCTGTTTT TAAGAGCATC ACCAATGACG AGGCCACCCA GGGTCAAGGC 
AGTCGGAGGC TGATCAGTTT TTCTCTCTCA GATTTCCAGG CCATGGGTCT GAAGAAAGGA 
ATGTTCTTTA ACCCAGATCC ATATCTGAAG ATTTCCATCC AACCTGGGAA ACACAGCATC 
TTCCCAGCCC TTCCGCACCA TGGTCAGGAG AAGAGGTCGA AGATTACCTG TAACACCGTG 
AACCCTGTTT GGCAAGCTGA GCAGTTTAGC TTTGTTTCCC TGCCCACCGA CGTCCTAGAA 
ATCGAGGTGA AAGACAAGTT TGCCAAGAGT CGTCCAATCA TCAAACGCTT CTTAGGAAAG 
TTGTCCATGC CTGTTCAGCG ACTCCTGGAG AGACACGCTA TAGGGGACAG GGTGGTGAGC 
TACACTCTGG GTCGAAGGCT TCCAACGGAT CACGTCAGCG GCCAGCTCCA GTTCCGGTTC 
GAGATCACCT CCTCCATCCA CCCAGATGAC GAGGAGGTCT CTCTGAGCGC AGATCCCGAG 
TCTGCTGAAA ACCAGGAGAG TCCTGCCAAC CACCCATCAG AGGAAGGCCT CACAGAACCT 
CCAGGCCAGG GGACGGAGGG CCCAGAGGGC CCGGAGCTGG AGACACAGGA TGGTGGTGGT 
CCCACGAGTG GCTCTGGGGG AGACAGCCCC GAGGCTACCG GACCGGCCGG GGAAAAGTCG 
AATGAGGAGG AGGCCGCAGA GGGTGCCGAA GCCGTCGTGG AGCCAGTTGA TGCCGTCGAT 
CCCACGGAAC CCAGCCCGGC CGAAGATTTG CCTTCCCTCA GTGCTGAAGA GATCGCCGAG 
GGGCTGGATC TGCTCCCTGG CACTCCCCCG CCACCGCCTC CGGAAGACGA GGGTTGGGAG 
CAGGGGGAGA ACCACGCCGG AGAGGCCGAT GGAGATCCCC TTGTCGGCCT GGAGGAGCCA 
GAGGGGGCTG AGGCAGAGGT GGAGAAAAAG GTTGACGTGA GCATGGTTCT AGACAAAGAC 
CAGGATGAAG AGGAAGAAGA ATTGGCCTCC CCCGAAGAGG ATGGGCCTCT GGCGCTGAGA 
CCCTCGGCCA AGAAGAAAAG CAGACCGTGC TCCCTGCCCG TGTCCGAGTT GGAGACGGTG 
ATCGCGTCGG CCTGCGGCGA ACCGGAAACG CCCCGCACCC ATTACATCCG CATCCACAAC 
CTGCTGCACA GCATGCCCTC AGCCCAGGGA GGCAGCGATG GCGGAGAAGC CCAAGGACCG 
GAGGAAGAGG ACGAGGAGGG GCCGGGGGAG GAAGAGTCAA CGGTCAAGGA GGCCTCCGAA 
AAGGACGTGG TCAGCGAGGC CGAGACCGTG GCACCGGACC CACCTCACCC AGGAGATGGC 
GGAGAGAGCG GGGACCCCGA GGTATCTGGC CAGCGGCCGG TGCCCCACAG TGCCTCGGCC 
GAGTGCTTGG CCGGGGTGAA CGGTGGCCCG CTTCTCGACG GCGAGCCCCG GCCCAGCACG 
GGGAGCGAGA GCGACTCGAG CCCCCGGCAG GACTGCGAGG GCTGCGACAC ATCCTGCTAC 
AGCCCGTCCT GCTACAGCAC CTCGTGTTAC AGCACCTCCT GCTACAGCAC CTCGTGCTAC 
AGCACCTCCT GCTACAGCCC ATCCTGCTAC GACAGCAACA ACCGGTTCTC CAGCCATACG 
CGCTTCTCCT CGGTGGACAG TGCCAAGATC TCGGAGAGCA CCGTCTTCTC CTCTCAGGAC 
GAGGAGGAGG AGGAGAACAG CGCGTTCGAG TCGGTGCCCG ACTCGGTGCA GAGCCCGGAG 
ATGGACCGGG AGACGGCCGA CGGCACCGGC CCGTGGCCGG ACGAGCTGGT TCTCCCCAAC 
GGGAACGTTC CGAGGACAGC CGATGGCCTG GAGTCCCCGA TAGCTGGTCC CAGTAATCGG 
AGAGAAGCTG AAGTACTACA TTTTCATCAT ATCATTTCGT CGGCTTGGAT TTCACTAGCA 
GGTGATTGTC CTTTACTACA TAATTCTCAG CCAGTCAGCC AGCTTCCTTC CTTGAGGCCT 
GACCACCACC ATTATCCAAC TATCGATGAG CCTCTTCCGC CAAACTGGGA GGCTCGAATA 
GACAGTCACG GGAGAGTGTT TTACGTGGAC CACGTGAACC GAACGACGAC GTGGCAGCGG 
CCTACCACGG CCGCCACTCC AGACGGGATC CGCAGATCTG GCTCCGTACA GCAGATGGAA 
CAGCTGAACC GCCGGTACCA GAATATCCAG CGGACTATTG CTACCGAGAG ATCGGAAGAC 
GAGCCACCCA GTCAGAGTGC GGAGCCTCTC CCTGCCGGAG GAGGGAGTGG AGGAGGGGGA 
GGAGGGAGTG ACTTGGAAGC AGACTCTTCT CAACCAAATC TAGAACTGAG GAGAGAGGGG 
TCTCTTTCTC CTGTGAACTC CCAAAAAATC ACTTTGCTGC TTCAGTCCCC GGCCGTCAAG 
TTCATCACCA ACCCTGAGTT CTTCACCGTG CTGCATGCCA ACTATAGTGC CTATAGAGTC 
TTCACCAACA GCACCTGCTT GAAGCATATG ATTCTGAAGA TCCGCAGGGA TGCCCGGAAT 
TTCGAACGCT ACCAGCACAA CCGGGACCTG GTGAACTTCA TCAACATGTT TGCTGACACC 
CGTTTGGAAC TGCCTCGCGG CTGGGAAATC AAAACTGACC AGCAGGGCAA GTCCTTCTTC 
GTCGACCACA ACAGTCGAGC CACCACTTTC ATCGACCCCC GGATCCCTCT TCAGAACGGA 
CGTCTTCCGA ATCACCTGAC TCACCGGCAG CACCTTCAGC GACTCCGGAG CTACAGTGCC 
GGCGAGGCCT CAGAAGTCTC TCGGAACCGA GGAGCTTCTC TGTTGGCCAG GCCGGGTCAC 
AGCCTGGTAG CAGCCATCAG AAACCAACAT CACCATGAGA CGTTACCTCT GGCTTATAAT 
GACAAGATCG TGGCATTTCT CCGCCAGCCA AACATTTTTG AGATGCTGCA GGAGCGCCAG 
CCCAGCTTGG CCAGAAACCA CGCACTCAGG GAGAAAATCC ATTACATTCG GACAGAAGGT 
ACTCAAGGGC TTGAAAAATT GTCCTGTGAT GCAGATTTAG TAATACTGCT GAGCCTCTTT 
GAAGAAGAGA TCATGTCCTA CATACCCCTT CAGGCCGCTT TCCACCCGGG ATACAGCTTC 
TCTCCTCGCT GTTCACCGTG CTCTTCCCCT CAAAACTCTC CAGGCCTACA GCGAGCCAGT 
GTCAGGGCCC CCTCACCCTA CAGGAGAGAC TTTGAGGCCA AGCTCCGAAA TTTCTACCGA 
AAACTTGAAG CCAAAGGATT TGGTCAAGGT CCGGGTAAAA TCAAACTCAT CATAAGACGT 
GACCACTTGC TGGAGGGAAC CTTCAATCAG GTGATGGCTT ATTCCCGCAA AGAGCTCCAG 
CGGAACAAGC TCTACGTCAC ATTTGTTGGA GAGGAGGGCC TAGACTACAG TGGGCCCTCC 
CGGGAGTTCT TTTTCCTTTT GTCCCAGGAG CTCTTCAACC CCTACTACGG CCTGTTTGAG 
TATTCTGCCA ATGACACTTA CACCGTGCAG ATCAGCCCCA TGTCGGCCTT TGTGGAAAAT 
CATCTCGAGT GGTTCAGATT TAGCGGTCGC ATCCTTGGGC TTGCCCTGAT CCACCAGTAC 
CTCCTGGACG CTTTCTTCAC GAGGCCGTTC TACAAGGCCC TGCTGAGACT GCCCTGTGAT 
CTGAGCGACC TAGAGTATCT GGATGAGGAA TTCCACCAAA GCCTGCAGTG GATGAAGGAC 
AATAACATCA CGGATATCTT AGATCTCACT TTCACAGTGA ATGAAGAAGT GTTTGGACAG 
GTAACAGAAA GAGAGTTGAA ATCAGGGGGA GCCAACACCC AGGTGACAGA AAAAAATAAA 
AAGGAGTACA TTGAGAGAAT GGTGAAGTGG AGAGTGGAGC GGGGCGTGGT GCAGCAGACG 
GAAGCGTTAG TCCGAGGCTT CTATGAAGTT GTGGATTCAA GGCTCGTCTC CGTCTTTGAT 
GCCAGGGAGC TGGAGCTGGT GATCGCCGGC ACCGCCGAAA TAGACCTCAA TGACTGGCGC 
AATAACACCG AGTATCGAGG GGGTTATCAC GATGGTCATA TCGTGATCCG GTGGTTCTGG 
GCAGCTGTGG AGAGGTTCAA CAATGAGCAG AGACTGAGGT TGCTTCAGTT CGTCACGGGA 
ACATCGAGCG TACCCTATGA AGGTTTCGCC GCGCTCCGAG GAAGCAATGG GCTGAGGCGC 
TTCTGTATAG AGAAATGGGG GAAAATAACA TCCCTTCCCA GGGCACACAC GTGTTTCAAC 
CGCTTGGATC TGCCACCGTA CCCATCGTAT TCCATGCTGT ACGAGAAGCT GTTGACAGCA 
GTTGAGGAAA CGAGCACCTT TGGTCTGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_029063054.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028918887.1 Ornithorhynchus anatinus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X4, mRNA.

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CloneID	OOc127715
Clone ID Related Accession (Same CDS sequence)	XM_029063054.1
Accession Version	XM_029063054.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	E3 ubiquitin-protein ligase HECW1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041731.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTAATC TGTACCAGAA CAGGTTCCTG GGTCTGGCCG CCATGGCCTC GCCGTCGAGG 
AACTCCCAGA GCCGGCGCCG CTGTAAAGAG CCCCTGCGCT ACAGCTACAA CCCCGACCAG 
TTCCACAACA TGGACATCAG GAACGGCCCA CACGGGACCG TCACCATCCC CCGCTCCACC 
AGCGACACCG ACCTGGTCAC GTCCGACAGT CGGTCCACCC TTATGGTCAG CAGTTCGTAT 
TACTCCATTG GACACTCTCA GGACCTGGTG ATCTATTGGG ATATAAAAGA GGAAGTGGGA 
GCCGGGGACT GGATTGGCAT GTATCTCATC GATGAGGTCT TATCTGAAAA TTTTCTGGAC 
TACAAAAACC GCGGAGTGAA TGGTTCTCAC CGGGGCCAGA TTATCTGGAA GATTGATGCA 
AGTTCTTACT TTGTGGAATC TGAAACTAAG ATCTGCTTCA AATACTATCA TGGAGTGAGT 
GGAGCGCTAC GAGCCACTAC GCCCAGTGTT ACAGTGAAAA ACTCTTCAGC TCCTGTTTTT 
AAGAGCATCA CCAATGACGA GGCCACCCAG GGTCAAGGCA GTCGGAGGCT GATCAGTTTT 
TCTCTCTCAG ATTTCCAGGC CATGGGTCTG AAGAAAGGAA TGTTCTTTAA CCCAGATCCA 
TATCTGAAGA TTTCCATCCA ACCTGGGAAA CACAGCATCT TCCCAGCCCT TCCGCACCAT 
GGTCAGGAGA AGAGGTCGAA GATTACCTGT AACACCGTGA ACCCTGTTTG GCAAGCTGAG 
CAGTTTAGCT TTGTTTCCCT GCCCACCGAC GTCCTAGAAA TCGAGGTGAA AGACAAGTTT 
GCCAAGAGTC GTCCAATCAT CAAACGCTTC TTAGGAAAGT TGTCCATGCC TGTTCAGCGA 
CTCCTGGAGA GACACGCTAT AGGGGACAGG GTGGTGAGCT ACACTCTGGG TCGAAGGCTT 
CCAACGGATC ACGTCAGCGG CCAGCTCCAG TTCCGGTTCG AGATCACCTC CTCCATCCAC 
CCAGATGACG AGGAGGTCTC TCTGAGCGCA GATCCCGAGT CTGCTGAAAA CCAGGAGAGT 
CCTGCCAACC ACCCATCAGA GGAAGGCCTC ACAGAACCTC CAGGCCAGGG GACGGAGGGC 
CCAGAGGGCC CGGAGCTGGA GACACAGGAT GGTGGTGGTC CCACGAGTGG CTCTGGGGGA 
GACAGCCCCG AGGCTACCGG ACCGGCCGGG GAAAAGTCGA ATGAGGAGGA GGCCGCAGAG 
GGTGCCGAAG CCGTCGTGGA GCCAGTTGAT GCCGTCGATC CCACGGAACC CAGCCCGGCC 
GAAGATTTGC CTTCCCTCAG TGCTGAAGAG ATCGCCGAGG GGCTGGATCT GCTCCCTGGC 
ACTCCCCCGC CACCGCCTCC GGAAGACGAG GGTTGGGAGC AGGGGGAGAA CCACGCCGGA 
GAGGCCGATG GAGATCCCCT TGTCGGCCTG GAGGAGCCAG AGGGGGCTGA GGCAGAGGTG 
GAGAAAAAGG TTGACGTGAG CATGGTTCTA GACAAAGACC AGGATGAAGA GGAAGAAGAA 
TTGGCCTCCC CCGAAGAGGA TGGGCCTCTG GCGCTGAGAC CCTCGGCCAA GAAGAAAAGC 
AGACCGTGCT CCCTGCCCGT GTCCGAGTTG GAGACGGTGA TCGCGTCGGC CTGCGGCGAA 
CCGGAAACGC CCCGCACCCA TTACATCCGC ATCCACAACC TGCTGCACAG CATGCCCTCA 
GCCCAGGGAG GCAGCGATGG CGGAGAAGCC CAAGGACCGG AGGAAGAGGA CGAGGAGGGG 
CCGGGGGAGG AAGAGTCAAC GGTCAAGGAG GCCTCCGAAA AGGACGTGGT CAGCGAGGCC 
GAGACCGTGG CACCGGACCC ACCTCACCCA GGAGATGGCG GAGAGAGCGG GGACCCCGAG 
GTATCTGGCC AGCGGCCGGT GCCCCACAGT GCCTCGGCCG AGTGCTTGGC CGGGGTGAAC 
GGTGGCCCGC TTCTCGACGG CGAGCCCCGG CCCAGCACGG GGAGCGAGAG CGACTCGAGC 
CCCCGGCAGG ACTGCGAGGG CTGCGACACA TCCTGCTACA GCCCGTCCTG CTACAGCACC 
TCGTGTTACA GCACCTCCTG CTACAGCACC TCGTGCTACA GCACCTCCTG CTACAGCCCA 
TCCTGCTACG ACAGCAACAA CCGGTTCTCC AGCCATACGC GCTTCTCCTC GGTGGACAGT 
GCCAAGATCT CGGAGAGCAC CGTCTTCTCC TCTCAGGACG AGGAGGAGGA GGAGAACAGC 
GCGTTCGAGT CGGTGCCCGA CTCGGTGCAG AGCCCGGAGA TGGACCGGGA GACGGCCGAC 
GGCACCGGCC CGTGGCCGGA CGAGCTGGTT CTCCCCAACG GGAACGTTCC GAGGACAGCC 
GATGGCCTGG AGTCCCCGAT AGCTGGTCCC AGTAATCGGA GAGAAGCTGA AGTACTACAT 
TTTCATCATA TCATTTCGTC GGCTTGGATT TCACTAGCAG GTGATTGTCC TTTACTACAT 
AATTCTCAGC CAGTCAGCCA GCTTCCTTCC TTGAGGCCTG ACCACCACCA TTATCCAACT 
ATCGATGAGC CTCTTCCGCC AAACTGGGAG GCTCGAATAG ACAGTCACGG GAGAGTGTTT 
TACGTGGACC ACGTGAACCG AACGACGACG TGGCAGCGGC CTACCACGGC CGCCACTCCA 
GACGGGATCC GCAGATCTGG CTCCGTACAG CAGATGGAAC AGCTGAACCG CCGGTACCAG 
AATATCCAGC GGACTATTGC TACCGAGAGA TCGGAAGACG AGCCACCCAG TCAGAGTGCG 
GAGCCTCTCC CTGCCGGAGG AGGGAGTGGA GGAGGGGGAG GAGGGAGTGA CTTGGAAGCA 
GACTCTTCTC AACCAAATCT AGAACTGAGG AGAGAGGGGT CTCTTTCTCC TGTGAACTCC 
CAAAAAATCA CTTTGCTGCT TCAGTCCCCG GCCGTCAAGT TCATCACCAA CCCTGAGTTC 
TTCACCGTGC TGCATGCCAA CTATAGTGCC TATAGAGTCT TCACCAACAG CACCTGCTTG 
AAGCATATGA TTCTGAAGAT CCGCAGGGAT GCCCGGAATT TCGAACGCTA CCAGCACAAC 
CGGGACCTGG TGAACTTCAT CAACATGTTT GCTGACACCC GTTTGGAACT GCCTCGCGGC 
TGGGAAATCA AAACTGACCA GCAGGGCAAG TCCTTCTTCG TCGACCACAA CAGTCGAGCC 
ACCACTTTCA TCGACCCCCG GATCCCTCTT CAGAACGGAC GTCTTCCGAA TCACCTGACT 
CACCGGCAGC ACCTTCAGCG ACTCCGGAGC TACAGTGCCG GCGAGGCCTC AGAAGTCTCT 
CGGAACCGAG GAGCTTCTCT GTTGGCCAGG CCGGGTCACA GCCTGGTAGC AGCCATCAGA 
AACCAACATC ACCATGAGAC GTTACCTCTG GCTTATAATG ACAAGATCGT GGCATTTCTC 
CGCCAGCCAA ACATTTTTGA GATGCTGCAG GAGCGCCAGC CCAGCTTGGC CAGAAACCAC 
GCACTCAGGG AGAAAATCCA TTACATTCGG ACAGAAGGTA CTCAAGGGCT TGAAAAATTG 
TCCTGTGATG CAGATTTAGT AATACTGCTG AGCCTCTTTG AAGAAGAGAT CATGTCCTAC 
ATACCCCTTC AGGCCGCTTT CCACCCGGGA TACAGCTTCT CTCCTCGCTG TTCACCGTGC 
TCTTCCCCTC AAAACTCTCC AGGCCTACAG CGAGCCAGTG TCAGGGCCCC CTCACCCTAC 
AGGAGAGACT TTGAGGCCAA GCTCCGAAAT TTCTACCGAA AACTTGAAGC CAAAGGATTT 
GGTCAAGGTC CGGGTAAAAT CAAACTCATC ATAAGACGTG ACCACTTGCT GGAGGGAACC 
TTCAATCAGG TGATGGCTTA TTCCCGCAAA GAGCTCCAGC GGAACAAGCT CTACGTCACA 
TTTGTTGGAG AGGAGGGCCT AGACTACAGT GGGCCCTCCC GGGAGTTCTT TTTCCTTTTG 
TCCCAGGAGC TCTTCAACCC CTACTACGGC CTGTTTGAGT ATTCTGCCAA TGACACTTAC 
ACCGTGCAGA TCAGCCCCAT GTCGGCCTTT GTGGAAAATC ATCTCGAGTG GTTCAGATTT 
AGCGGTCGCA TCCTTGGGCT TGCCCTGATC CACCAGTACC TCCTGGACGC TTTCTTCACG 
AGGCCGTTCT ACAAGGCCCT GCTGAGACTG CCCTGTGATC TGAGCGACCT AGAGTATCTG 
GATGAGGAAT TCCACCAAAG CCTGCAGTGG ATGAAGGACA ATAACATCAC GGATATCTTA 
GATCTCACTT TCACAGTGAA TGAAGAAGTG TTTGGACAGG TAACAGAAAG AGAGTTGAAA 
TCAGGGGGAG CCAACACCCA GGTGACAGAA AAAAATAAAA AGGAGTACAT TGAGAGAATG 
GTGAAGTGGA GAGTGGAGCG GGGCGTGGTG CAGCAGACGG AAGCGTTAGT CCGAGGCTTC 
TATGAAGTTG TGGATTCAAG GCTCGTCTCC GTCTTTGATG CCAGGGAGCT GGAGCTGGTG 
ATCGCCGGCA CCGCCGAAAT AGACCTCAAT GACTGGCGCA ATAACACCGA GTATCGAGGG 
GGTTATCACG ATGGTCATAT CGTGATCCGG TGGTTCTGGG CAGCTGTGGA GAGGTTCAAC 
AATGAGCAGA GACTGAGGTT GCTTCAGTTC GTCACGGGAA CATCGAGCGT ACCCTATGAA 
GGTTTCGCCG CGCTCCGAGG AAGCAATGGG CTGAGGCGCT TCTGTATAGA GAAATGGGGG 
AAAATAACAT CCCTTCCCAG GGCACACACG TGTTTCAACC GCTTGGATCT GCCACCGTAC 
CCATCGTATT CCATGCTGTA CGAGAAGCTG TTGACAGCAG TTGAGGAAAC GAGCACCTTT 
GGTCTGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028918887.1
CDS	235..5166
Translation	MINLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDIRNGPHGTVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIYWDIKEEVGAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSSAPVFKSITNDEATQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQEKRSKITCNTVNPVWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEVSLSADPESAENQESPANHPSEEGLTEPPGQGTEGPEGPELETQDGGGPTSGSGGDSPEATGPAGEKSNEEEAAEGAEAVVEPVDAVDPTEPSPAEDLPSLSAEEIAEGLDLLPGTPPPPPPEDEGWEQGENHAGEADGDPLVGLEEPEGAEAEVEKKVDVSMVLDKDQDEEEEELASPEEDGPLALRPSAKKKSRPCSLPVSELETVIASACGEPETPRTHYIRIHNLLHSMPSAQGGSDGGEAQGPEEEDEEGPGEEESTVKEASEKDVVSEAETVAPDPPHPGDGGESGDPEVSGQRPVPHSASAECLAGVNGGPLLDGEPRPSTGSESDSSPRQDCEGCDTSCYSPSCYSTSCYSTSCYSTSCYSTSCYSPSCYDSNNRFSSHTRFSSVDSAKISESTVFSSQDEEEEENSAFESVPDSVQSPEMDRETADGTGPWPDELVLPNGNVPRTADGLESPIAGPSNRREAEVLHFHHIISSAWISLAGDCPLLHNSQPVSQLPSLRPDHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTTAATPDGIRRSGSVQQMEQLNRRYQNIQRTIATERSEDEPPSQSAEPLPAGGGSGGGGGGSDLEADSSQPNLELRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTNSTCLKHMILKIRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRNQHHHETLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHALREKIHYIRTEGTQGLEKLSCDADLVILLSLFEEEIMSYIPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASVRAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_029063054.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029063054.1

ORF Insert Sequence:

ATGATTAATC TGTACCAGAA CAGGTTCCTG GGTCTGGCCG CCATGGCCTC GCCGTCGAGG 
AACTCCCAGA GCCGGCGCCG CTGTAAAGAG CCCCTGCGCT ACAGCTACAA CCCCGACCAG 
TTCCACAACA TGGACATCAG GAACGGCCCA CACGGGACCG TCACCATCCC CCGCTCCACC 
AGCGACACCG ACCTGGTCAC GTCCGACAGT CGGTCCACCC TTATGGTCAG CAGTTCGTAT 
TACTCCATTG GACACTCTCA GGACCTGGTG ATCTATTGGG ATATAAAAGA GGAAGTGGGA 
GCCGGGGACT GGATTGGCAT GTATCTCATC GATGAGGTCT TATCTGAAAA TTTTCTGGAC 
TACAAAAACC GCGGAGTGAA TGGTTCTCAC CGGGGCCAGA TTATCTGGAA GATTGATGCA 
AGTTCTTACT TTGTGGAATC TGAAACTAAG ATCTGCTTCA AATACTATCA TGGAGTGAGT 
GGAGCGCTAC GAGCCACTAC GCCCAGTGTT ACAGTGAAAA ACTCTTCAGC TCCTGTTTTT 
AAGAGCATCA CCAATGACGA GGCCACCCAG GGTCAAGGCA GTCGGAGGCT GATCAGTTTT 
TCTCTCTCAG ATTTCCAGGC CATGGGTCTG AAGAAAGGAA TGTTCTTTAA CCCAGATCCA 
TATCTGAAGA TTTCCATCCA ACCTGGGAAA CACAGCATCT TCCCAGCCCT TCCGCACCAT 
GGTCAGGAGA AGAGGTCGAA GATTACCTGT AACACCGTGA ACCCTGTTTG GCAAGCTGAG 
CAGTTTAGCT TTGTTTCCCT GCCCACCGAC GTCCTAGAAA TCGAGGTGAA AGACAAGTTT 
GCCAAGAGTC GTCCAATCAT CAAACGCTTC TTAGGAAAGT TGTCCATGCC TGTTCAGCGA 
CTCCTGGAGA GACACGCTAT AGGGGACAGG GTGGTGAGCT ACACTCTGGG TCGAAGGCTT 
CCAACGGATC ACGTCAGCGG CCAGCTCCAG TTCCGGTTCG AGATCACCTC CTCCATCCAC 
CCAGATGACG AGGAGGTCTC TCTGAGCGCA GATCCCGAGT CTGCTGAAAA CCAGGAGAGT 
CCTGCCAACC ACCCATCAGA GGAAGGCCTC ACAGAACCTC CAGGCCAGGG GACGGAGGGC 
CCAGAGGGCC CGGAGCTGGA GACACAGGAT GGTGGTGGTC CCACGAGTGG CTCTGGGGGA 
GACAGCCCCG AGGCTACCGG ACCGGCCGGG GAAAAGTCGA ATGAGGAGGA GGCCGCAGAG 
GGTGCCGAAG CCGTCGTGGA GCCAGTTGAT GCCGTCGATC CCACGGAACC CAGCCCGGCC 
GAAGATTTGC CTTCCCTCAG TGCTGAAGAG ATCGCCGAGG GGCTGGATCT GCTCCCTGGC 
ACTCCCCCGC CACCGCCTCC GGAAGACGAG GGTTGGGAGC AGGGGGAGAA CCACGCCGGA 
GAGGCCGATG GAGATCCCCT TGTCGGCCTG GAGGAGCCAG AGGGGGCTGA GGCAGAGGTG 
GAGAAAAAGG TTGACGTGAG CATGGTTCTA GACAAAGACC AGGATGAAGA GGAAGAAGAA 
TTGGCCTCCC CCGAAGAGGA TGGGCCTCTG GCGCTGAGAC CCTCGGCCAA GAAGAAAAGC 
AGACCGTGCT CCCTGCCCGT GTCCGAGTTG GAGACGGTGA TCGCGTCGGC CTGCGGCGAA 
CCGGAAACGC CCCGCACCCA TTACATCCGC ATCCACAACC TGCTGCACAG CATGCCCTCA 
GCCCAGGGAG GCAGCGATGG CGGAGAAGCC CAAGGACCGG AGGAAGAGGA CGAGGAGGGG 
CCGGGGGAGG AAGAGTCAAC GGTCAAGGAG GCCTCCGAAA AGGACGTGGT CAGCGAGGCC 
GAGACCGTGG CACCGGACCC ACCTCACCCA GGAGATGGCG GAGAGAGCGG GGACCCCGAG 
GTATCTGGCC AGCGGCCGGT GCCCCACAGT GCCTCGGCCG AGTGCTTGGC CGGGGTGAAC 
GGTGGCCCGC TTCTCGACGG CGAGCCCCGG CCCAGCACGG GGAGCGAGAG CGACTCGAGC 
CCCCGGCAGG ACTGCGAGGG CTGCGACACA TCCTGCTACA GCCCGTCCTG CTACAGCACC 
TCGTGTTACA GCACCTCCTG CTACAGCACC TCGTGCTACA GCACCTCCTG CTACAGCCCA 
TCCTGCTACG ACAGCAACAA CCGGTTCTCC AGCCATACGC GCTTCTCCTC GGTGGACAGT 
GCCAAGATCT CGGAGAGCAC CGTCTTCTCC TCTCAGGACG AGGAGGAGGA GGAGAACAGC 
GCGTTCGAGT CGGTGCCCGA CTCGGTGCAG AGCCCGGAGA TGGACCGGGA GACGGCCGAC 
GGCACCGGCC CGTGGCCGGA CGAGCTGGTT CTCCCCAACG GGAACGTTCC GAGGACAGCC 
GATGGCCTGG AGTCCCCGAT AGCTGGTCCC AGTAATCGGA GAGAAGCTGA AGTACTACAT 
TTTCATCATA TCATTTCGTC GGCTTGGATT TCACTAGCAG GTGATTGTCC TTTACTACAT 
AATTCTCAGC CAGTCAGCCA GCTTCCTTCC TTGAGGCCTG ACCACCACCA TTATCCAACT 
ATCGATGAGC CTCTTCCGCC AAACTGGGAG GCTCGAATAG ACAGTCACGG GAGAGTGTTT 
TACGTGGACC ACGTGAACCG AACGACGACG TGGCAGCGGC CTACCACGGC CGCCACTCCA 
GACGGGATCC GCAGATCTGG CTCCGTACAG CAGATGGAAC AGCTGAACCG CCGGTACCAG 
AATATCCAGC GGACTATTGC TACCGAGAGA TCGGAAGACG AGCCACCCAG TCAGAGTGCG 
GAGCCTCTCC CTGCCGGAGG AGGGAGTGGA GGAGGGGGAG GAGGGAGTGA CTTGGAAGCA 
GACTCTTCTC AACCAAATCT AGAACTGAGG AGAGAGGGGT CTCTTTCTCC TGTGAACTCC 
CAAAAAATCA CTTTGCTGCT TCAGTCCCCG GCCGTCAAGT TCATCACCAA CCCTGAGTTC 
TTCACCGTGC TGCATGCCAA CTATAGTGCC TATAGAGTCT TCACCAACAG CACCTGCTTG 
AAGCATATGA TTCTGAAGAT CCGCAGGGAT GCCCGGAATT TCGAACGCTA CCAGCACAAC 
CGGGACCTGG TGAACTTCAT CAACATGTTT GCTGACACCC GTTTGGAACT GCCTCGCGGC 
TGGGAAATCA AAACTGACCA GCAGGGCAAG TCCTTCTTCG TCGACCACAA CAGTCGAGCC 
ACCACTTTCA TCGACCCCCG GATCCCTCTT CAGAACGGAC GTCTTCCGAA TCACCTGACT 
CACCGGCAGC ACCTTCAGCG ACTCCGGAGC TACAGTGCCG GCGAGGCCTC AGAAGTCTCT 
CGGAACCGAG GAGCTTCTCT GTTGGCCAGG CCGGGTCACA GCCTGGTAGC AGCCATCAGA 
AACCAACATC ACCATGAGAC GTTACCTCTG GCTTATAATG ACAAGATCGT GGCATTTCTC 
CGCCAGCCAA ACATTTTTGA GATGCTGCAG GAGCGCCAGC CCAGCTTGGC CAGAAACCAC 
GCACTCAGGG AGAAAATCCA TTACATTCGG ACAGAAGGTA CTCAAGGGCT TGAAAAATTG 
TCCTGTGATG CAGATTTAGT AATACTGCTG AGCCTCTTTG AAGAAGAGAT CATGTCCTAC 
ATACCCCTTC AGGCCGCTTT CCACCCGGGA TACAGCTTCT CTCCTCGCTG TTCACCGTGC 
TCTTCCCCTC AAAACTCTCC AGGCCTACAG CGAGCCAGTG TCAGGGCCCC CTCACCCTAC 
AGGAGAGACT TTGAGGCCAA GCTCCGAAAT TTCTACCGAA AACTTGAAGC CAAAGGATTT 
GGTCAAGGTC CGGGTAAAAT CAAACTCATC ATAAGACGTG ACCACTTGCT GGAGGGAACC 
TTCAATCAGG TGATGGCTTA TTCCCGCAAA GAGCTCCAGC GGAACAAGCT CTACGTCACA 
TTTGTTGGAG AGGAGGGCCT AGACTACAGT GGGCCCTCCC GGGAGTTCTT TTTCCTTTTG 
TCCCAGGAGC TCTTCAACCC CTACTACGGC CTGTTTGAGT ATTCTGCCAA TGACACTTAC 
ACCGTGCAGA TCAGCCCCAT GTCGGCCTTT GTGGAAAATC ATCTCGAGTG GTTCAGATTT 
AGCGGTCGCA TCCTTGGGCT TGCCCTGATC CACCAGTACC TCCTGGACGC TTTCTTCACG 
AGGCCGTTCT ACAAGGCCCT GCTGAGACTG CCCTGTGATC TGAGCGACCT AGAGTATCTG 
GATGAGGAAT TCCACCAAAG CCTGCAGTGG ATGAAGGACA ATAACATCAC GGATATCTTA 
GATCTCACTT TCACAGTGAA TGAAGAAGTG TTTGGACAGG TAACAGAAAG AGAGTTGAAA 
TCAGGGGGAG CCAACACCCA GGTGACAGAA AAAAATAAAA AGGAGTACAT TGAGAGAATG 
GTGAAGTGGA GAGTGGAGCG GGGCGTGGTG CAGCAGACGG AAGCGTTAGT CCGAGGCTTC 
TATGAAGTTG TGGATTCAAG GCTCGTCTCC GTCTTTGATG CCAGGGAGCT GGAGCTGGTG 
ATCGCCGGCA CCGCCGAAAT AGACCTCAAT GACTGGCGCA ATAACACCGA GTATCGAGGG 
GGTTATCACG ATGGTCATAT CGTGATCCGG TGGTTCTGGG CAGCTGTGGA GAGGTTCAAC 
AATGAGCAGA GACTGAGGTT GCTTCAGTTC GTCACGGGAA CATCGAGCGT ACCCTATGAA 
GGTTTCGCCG CGCTCCGAGG AAGCAATGGG CTGAGGCGCT TCTGTATAGA GAAATGGGGG 
AAAATAACAT CCCTTCCCAG GGCACACACG TGTTTCAACC GCTTGGATCT GCCACCGTAC 
CCATCGTATT CCATGCTGTA CGAGAAGCTG TTGACAGCAG TTGAGGAAAC GAGCACCTTT 
GGTCTGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_029063056.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028918889.1 Ornithorhynchus anatinus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X5, mRNA.

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CloneID	OOc127716
Clone ID Related Accession (Same CDS sequence)	XM_029063056.1
Accession Version	XM_029063056.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	E3 ubiquitin-protein ligase HECW1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041731.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGTG CCCAGCCTGG CATCATTCAG AATCTGTACC AGAACAGGTT CCTGGGTCTG 
GCCGCCATGG CCTCGCCGTC GAGGAACTCC CAGAGCCGGC GCCGCTGTAA AGAGCCCCTG 
CGCTACAGCT ACAACCCCGA CCAGTTCCAC AACATGGACA TCAGGAACGG CCCACACGGG 
ACCGTCACCA TCCCCCGCTC CACCAGCGAC ACCGACCTGG TCACGTCCGA CAGTCGGTCC 
ACCCTTATGG TCAGCAGTTC GTATTACTCC ATTGGACACT CTCAGGACCT GGTGATCTAT 
TGGGATATAA AAGAGGAAGT GGGAGCCGGG GACTGGATTG GCATGTATCT CATCGATGAG 
GTCTTATCTG AAAATTTTCT GGACTACAAA AACCGCGGAG TGAATGGTTC TCACCGGGGC 
CAGATTATCT GGAAGATTGA TGCAAGTTCT TACTTTGTGG AATCTGAAAC TAAGATCTGC 
TTCAAATACT ATCATGGAGT GAGTGGAGCG CTACGAGCCA CTACGCCCAG TGTTACAGTG 
AAAAACTCTT CAGCTCCTGT TTTTAAGAGC ATCACCAATG ACGAGGCCAC CCAGGGTCAA 
GGCAGTCGGA GGCTGATCAG TTTTTCTCTC TCAGATTTCC AGGCCATGGG TCTGAAGAAA 
GGAATGTTCT TTAACCCAGA TCCATATCTG AAGATTTCCA TCCAACCTGG GAAACACAGC 
ATCTTCCCAG CCCTTCCGCA CCATGGTCAG GAGAAGAGGT CGAAGATTAC CTGTAACACC 
GTGAACCCTG TTTGGCAAGC TGAGCAGTTT AGCTTTGTTT CCCTGCCCAC CGACGTCCTA 
GAAATCGAGG TGAAAGACAA GTTTGCCAAG AGTCGTCCAA TCATCAAACG CTTCTTAGGA 
AAGTTGTCCA TGCCTGTTCA GCGACTCCTG GAGAGACACG CTATAGGGGA CAGGGTGGTG 
AGCTACACTC TGGGTCGAAG GCTTCCAACG GATCACGTCA GCGGCCAGCT CCAGTTCCGG 
TTCGAGATCA CCTCCTCCAT CCACCCAGAT GACGAGGAGG TCTCTCTGAG CGCAGATCCC 
GAGTCTGCTG AAAACCAGGA GAGTCCTGCC AACCACCCAT CAGAGGAAGG CCTCACAGAA 
CCTCCAGGCC AGGGGACGGA GGGCCCAGAG GGCCCGGAGC TGGAGACACA GGATGGTGGT 
GGTCCCACGA GTGGCTCTGG GGGAGACAGC CCCGAGGCTA CCGGACCGGC CGGGGAAAAG 
TCGAATGAGG AGGAGGCCGC AGAGGGTGCC GAAGCCGTCG TGGAGCCAGT TGATGCCGTC 
GATCCCACGG AACCCAGCCC GGCCGAAGAT TTGCCTTCCC TCAGTGCTGA AGAGATCGCC 
GAGGGGCTGG ATCTGCTCCC TGGCACTCCC CCGCCACCGC CTCCGGAAGA CGAGGGTTGG 
GAGCAGGGGG AGAACCACGC CGGAGAGGCC GATGGAGATC CCCTTGTCGG CCTGGAGGAG 
CCAGAGGGGG CTGAGGCAGA GGTGGAGAAA AAGGTTGACG TGAGCATGGT TCTAGACAAA 
GACCAGGATG AAGAGGAAGA AGAATTGGCC TCCCCCGAAG AGGATGGGCC TCTGGCGCTG 
AGACCCTCGG CCAAGAAGAA AAGCAGACCG TGCTCCCTGC CCGTGTCCGA GTTGGAGACG 
GTGATCGCGT CGGCCTGCGG CGAACCGGAA ACGCCCCGCA CCCATTACAT CCGCATCCAC 
AACCTGCTGC ACAGCATGCC CTCAGCCCAG GGAGGCAGCG ATGGCGGAGA AGCCCAAGGA 
CCGGAGGAAG AGGACGAGGA GGGGCCGGGG GAGGAAGAGT CAACGGTCAA GGAGGCCTCC 
GAAAAGGACG TGGTCAGCGA GGCCGAGACC GTGGCACCGG ACCCACCTCA CCCAGGAGAT 
GGCGGAGAGA GCGGGGACCC CGAGGTATCT GGCCAGCGGC CGGTGCCCCA CAGTGCCTCG 
GCCGAGTGCT TGGCCGGGGT GAACGGTGGC CCGCTTCTCG ACGGCGAGCC CCGGCCCAGC 
ACGGGGAGCG AGAGCGACTC GAGCCCCCGG CAGGACTGCG AGGGCTGCGA CACATCCTGC 
TACAGCCCGT CCTGCTACAG CACCTCGTGT TACAGCACCT CCTGCTACAG CACCTCGTGC 
TACAGCACCT CCTGCTACAG CCCATCCTGC TACGACAGCA ACAACCGGTT CTCCAGCCAT 
ACGCGCTTCT CCTCGGTGGA CAGTGCCAAG ATCTCGGAGA GCACCGTCTT CTCCTCTCAG 
GACGAGGAGG AGGAGGAGAA CAGCGCGTTC GAGTCGGTGC CCGACTCGGT GCAGAGCCCG 
GAGATGGACC GGGAGACGGC CGACGGCACC GGCCCGTGGC CGGACGAGCT GGTTCTCCCC 
AACGGGAACG TTCCGAGGAC AGCCGATGGC CTGGAGTCCC CGATAGCTGG TCCCAGTAAT 
CGGAGAGAAG GTGATTGTCC TTTACTACAT AATTCTCAGC CAGTCAGCCA GCTTCCTTCC 
TTGAGGCCTG ACCACCACCA TTATCCAACT ATCGATGAGC CTCTTCCGCC AAACTGGGAG 
GCTCGAATAG ACAGTCACGG GAGAGTGTTT TACGTGGACC ACGTGAACCG AACGACGACG 
TGGCAGCGGC CTACCACGGC CGCCACTCCA GACGGGATCC GCAGATCTGG CTCCGTACAG 
CAGATGGAAC AGCTGAACCG CCGGTACCAG AATATCCAGC GGACTATTGC TACCGAGAGA 
TCGGAAGACG AGCCACCCAG TCAGAGTGCG GAGCCTCTCC CTGCCGGAGG AGGGAGTGGA 
GGAGGGGGAG GAGGGAGTGA CTTGGAAGCA GACTCTTCTC AACCAAATCT AGAACTGAGG 
AGAGAGGGGT CTCTTTCTCC TGTGAACTCC CAAAAAATCA CTTTGCTGCT TCAGTCCCCG 
GCCGTCAAGT TCATCACCAA CCCTGAGTTC TTCACCGTGC TGCATGCCAA CTATAGTGCC 
TATAGAGTCT TCACCAACAG CACCTGCTTG AAGCATATGA TTCTGAAGAT CCGCAGGGAT 
GCCCGGAATT TCGAACGCTA CCAGCACAAC CGGGACCTGG TGAACTTCAT CAACATGTTT 
GCTGACACCC GTTTGGAACT GCCTCGCGGC TGGGAAATCA AAACTGACCA GCAGGGCAAG 
TCCTTCTTCG TCGACCACAA CAGTCGAGCC ACCACTTTCA TCGACCCCCG GATCCCTCTT 
CAGAACGGAC GTCTTCCGAA TCACCTGACT CACCGGCAGC ACCTTCAGCG ACTCCGGAGC 
TACAGTGCCG GCGAGGCCTC AGAAGTCTCT CGGAACCGAG GAGCTTCTCT GTTGGCCAGG 
CCGGGTCACA GCCTGGTAGC AGCCATCAGA AACCAACATC ACCATGAGAC GTTACCTCTG 
GCTTATAATG ACAAGATCGT GGCATTTCTC CGCCAGCCAA ACATTTTTGA GATGCTGCAG 
GAGCGCCAGC CCAGCTTGGC CAGAAACCAC GCACTCAGGG AGAAAATCCA TTACATTCGG 
ACAGAAGGTA CTCAAGGGCT TGAAAAATTG TCCTGTGATG CAGATTTAGT AATACTGCTG 
AGCCTCTTTG AAGAAGAGAT CATGTCCTAC ATACCCCTTC AGGCCGCTTT CCACCCGGGA 
TACAGCTTCT CTCCTCGCTG TTCACCGTGC TCTTCCCCTC AAAACTCTCC AGGCCTACAG 
CGAGCCAGTG TCAGGGCCCC CTCACCCTAC AGGAGAGACT TTGAGGCCAA GCTCCGAAAT 
TTCTACCGAA AACTTGAAGC CAAAGGATTT GGTCAAGGTC CGGGTAAAAT CAAACTCATC 
ATAAGACGTG ACCACTTGCT GGAGGGAACC TTCAATCAGG TGATGGCTTA TTCCCGCAAA 
GAGCTCCAGC GGAACAAGCT CTACGTCACA TTTGTTGGAG AGGAGGGCCT AGACTACAGT 
GGGCCCTCCC GGGAGTTCTT TTTCCTTTTG TCCCAGGAGC TCTTCAACCC CTACTACGGC 
CTGTTTGAGT ATTCTGCCAA TGACACTTAC ACCGTGCAGA TCAGCCCCAT GTCGGCCTTT 
GTGGAAAATC ATCTCGAGTG GTTCAGATTT AGCGGTCGCA TCCTTGGGCT TGCCCTGATC 
CACCAGTACC TCCTGGACGC TTTCTTCACG AGGCCGTTCT ACAAGGCCCT GCTGAGACTG 
CCCTGTGATC TGAGCGACCT AGAGTATCTG GATGAGGAAT TCCACCAAAG CCTGCAGTGG 
ATGAAGGACA ATAACATCAC GGATATCTTA GATCTCACTT TCACAGTGAA TGAAGAAGTG 
TTTGGACAGG TAACAGAAAG AGAGTTGAAA TCAGGGGGAG CCAACACCCA GGTGACAGAA 
AAAAATAAAA AGGAGTACAT TGAGAGAATG GTGAAGTGGA GAGTGGAGCG GGGCGTGGTG 
CAGCAGACGG AAGCGTTAGT CCGAGGCTTC TATGAAGTTG TGGATTCAAG GCTCGTCTCC 
GTCTTTGATG CCAGGGAGCT GGAGCTGGTG ATCGCCGGCA CCGCCGAAAT AGACCTCAAT 
GACTGGCGCA ATAACACCGA GTATCGAGGG GGTTATCACG ATGGTCATAT CGTGATCCGG 
TGGTTCTGGG CAGCTGTGGA GAGGTTCAAC AATGAGCAGA GACTGAGGTT GCTTCAGTTC 
GTCACGGGAA CATCGAGCGT ACCCTATGAA GGTTTCGCCG CGCTCCGAGG AAGCAATGGG 
CTGAGGCGCT TCTGTATAGA GAAATGGGGG AAAATAACAT CCCTTCCCAG GGCACACACG 
TGTTTCAACC GCTTGGATCT GCCACCGTAC CCATCGTATT CCATGCTGTA CGAGAAGCTG 
TTGACAGCAG TTGAGGAAAC GAGCACCTTT GGTCTGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028918889.1
CDS	103..5004
Translation	MLCAQPGIIQNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDIRNGPHGTVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIYWDIKEEVGAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSSAPVFKSITNDEATQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQEKRSKITCNTVNPVWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEVSLSADPESAENQESPANHPSEEGLTEPPGQGTEGPEGPELETQDGGGPTSGSGGDSPEATGPAGEKSNEEEAAEGAEAVVEPVDAVDPTEPSPAEDLPSLSAEEIAEGLDLLPGTPPPPPPEDEGWEQGENHAGEADGDPLVGLEEPEGAEAEVEKKVDVSMVLDKDQDEEEEELASPEEDGPLALRPSAKKKSRPCSLPVSELETVIASACGEPETPRTHYIRIHNLLHSMPSAQGGSDGGEAQGPEEEDEEGPGEEESTVKEASEKDVVSEAETVAPDPPHPGDGGESGDPEVSGQRPVPHSASAECLAGVNGGPLLDGEPRPSTGSESDSSPRQDCEGCDTSCYSPSCYSTSCYSTSCYSTSCYSTSCYSPSCYDSNNRFSSHTRFSSVDSAKISESTVFSSQDEEEEENSAFESVPDSVQSPEMDRETADGTGPWPDELVLPNGNVPRTADGLESPIAGPSNRREGDCPLLHNSQPVSQLPSLRPDHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTTAATPDGIRRSGSVQQMEQLNRRYQNIQRTIATERSEDEPPSQSAEPLPAGGGSGGGGGGSDLEADSSQPNLELRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTNSTCLKHMILKIRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRNQHHHETLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHALREKIHYIRTEGTQGLEKLSCDADLVILLSLFEEEIMSYIPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASVRAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_029063056.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029063056.1

ORF Insert Sequence:

ATGCTTTGTG CCCAGCCTGG CATCATTCAG AATCTGTACC AGAACAGGTT CCTGGGTCTG 
GCCGCCATGG CCTCGCCGTC GAGGAACTCC CAGAGCCGGC GCCGCTGTAA AGAGCCCCTG 
CGCTACAGCT ACAACCCCGA CCAGTTCCAC AACATGGACA TCAGGAACGG CCCACACGGG 
ACCGTCACCA TCCCCCGCTC CACCAGCGAC ACCGACCTGG TCACGTCCGA CAGTCGGTCC 
ACCCTTATGG TCAGCAGTTC GTATTACTCC ATTGGACACT CTCAGGACCT GGTGATCTAT 
TGGGATATAA AAGAGGAAGT GGGAGCCGGG GACTGGATTG GCATGTATCT CATCGATGAG 
GTCTTATCTG AAAATTTTCT GGACTACAAA AACCGCGGAG TGAATGGTTC TCACCGGGGC 
CAGATTATCT GGAAGATTGA TGCAAGTTCT TACTTTGTGG AATCTGAAAC TAAGATCTGC 
TTCAAATACT ATCATGGAGT GAGTGGAGCG CTACGAGCCA CTACGCCCAG TGTTACAGTG 
AAAAACTCTT CAGCTCCTGT TTTTAAGAGC ATCACCAATG ACGAGGCCAC CCAGGGTCAA 
GGCAGTCGGA GGCTGATCAG TTTTTCTCTC TCAGATTTCC AGGCCATGGG TCTGAAGAAA 
GGAATGTTCT TTAACCCAGA TCCATATCTG AAGATTTCCA TCCAACCTGG GAAACACAGC 
ATCTTCCCAG CCCTTCCGCA CCATGGTCAG GAGAAGAGGT CGAAGATTAC CTGTAACACC 
GTGAACCCTG TTTGGCAAGC TGAGCAGTTT AGCTTTGTTT CCCTGCCCAC CGACGTCCTA 
GAAATCGAGG TGAAAGACAA GTTTGCCAAG AGTCGTCCAA TCATCAAACG CTTCTTAGGA 
AAGTTGTCCA TGCCTGTTCA GCGACTCCTG GAGAGACACG CTATAGGGGA CAGGGTGGTG 
AGCTACACTC TGGGTCGAAG GCTTCCAACG GATCACGTCA GCGGCCAGCT CCAGTTCCGG 
TTCGAGATCA CCTCCTCCAT CCACCCAGAT GACGAGGAGG TCTCTCTGAG CGCAGATCCC 
GAGTCTGCTG AAAACCAGGA GAGTCCTGCC AACCACCCAT CAGAGGAAGG CCTCACAGAA 
CCTCCAGGCC AGGGGACGGA GGGCCCAGAG GGCCCGGAGC TGGAGACACA GGATGGTGGT 
GGTCCCACGA GTGGCTCTGG GGGAGACAGC CCCGAGGCTA CCGGACCGGC CGGGGAAAAG 
TCGAATGAGG AGGAGGCCGC AGAGGGTGCC GAAGCCGTCG TGGAGCCAGT TGATGCCGTC 
GATCCCACGG AACCCAGCCC GGCCGAAGAT TTGCCTTCCC TCAGTGCTGA AGAGATCGCC 
GAGGGGCTGG ATCTGCTCCC TGGCACTCCC CCGCCACCGC CTCCGGAAGA CGAGGGTTGG 
GAGCAGGGGG AGAACCACGC CGGAGAGGCC GATGGAGATC CCCTTGTCGG CCTGGAGGAG 
CCAGAGGGGG CTGAGGCAGA GGTGGAGAAA AAGGTTGACG TGAGCATGGT TCTAGACAAA 
GACCAGGATG AAGAGGAAGA AGAATTGGCC TCCCCCGAAG AGGATGGGCC TCTGGCGCTG 
AGACCCTCGG CCAAGAAGAA AAGCAGACCG TGCTCCCTGC CCGTGTCCGA GTTGGAGACG 
GTGATCGCGT CGGCCTGCGG CGAACCGGAA ACGCCCCGCA CCCATTACAT CCGCATCCAC 
AACCTGCTGC ACAGCATGCC CTCAGCCCAG GGAGGCAGCG ATGGCGGAGA AGCCCAAGGA 
CCGGAGGAAG AGGACGAGGA GGGGCCGGGG GAGGAAGAGT CAACGGTCAA GGAGGCCTCC 
GAAAAGGACG TGGTCAGCGA GGCCGAGACC GTGGCACCGG ACCCACCTCA CCCAGGAGAT 
GGCGGAGAGA GCGGGGACCC CGAGGTATCT GGCCAGCGGC CGGTGCCCCA CAGTGCCTCG 
GCCGAGTGCT TGGCCGGGGT GAACGGTGGC CCGCTTCTCG ACGGCGAGCC CCGGCCCAGC 
ACGGGGAGCG AGAGCGACTC GAGCCCCCGG CAGGACTGCG AGGGCTGCGA CACATCCTGC 
TACAGCCCGT CCTGCTACAG CACCTCGTGT TACAGCACCT CCTGCTACAG CACCTCGTGC 
TACAGCACCT CCTGCTACAG CCCATCCTGC TACGACAGCA ACAACCGGTT CTCCAGCCAT 
ACGCGCTTCT CCTCGGTGGA CAGTGCCAAG ATCTCGGAGA GCACCGTCTT CTCCTCTCAG 
GACGAGGAGG AGGAGGAGAA CAGCGCGTTC GAGTCGGTGC CCGACTCGGT GCAGAGCCCG 
GAGATGGACC GGGAGACGGC CGACGGCACC GGCCCGTGGC CGGACGAGCT GGTTCTCCCC 
AACGGGAACG TTCCGAGGAC AGCCGATGGC CTGGAGTCCC CGATAGCTGG TCCCAGTAAT 
CGGAGAGAAG GTGATTGTCC TTTACTACAT AATTCTCAGC CAGTCAGCCA GCTTCCTTCC 
TTGAGGCCTG ACCACCACCA TTATCCAACT ATCGATGAGC CTCTTCCGCC AAACTGGGAG 
GCTCGAATAG ACAGTCACGG GAGAGTGTTT TACGTGGACC ACGTGAACCG AACGACGACG 
TGGCAGCGGC CTACCACGGC CGCCACTCCA GACGGGATCC GCAGATCTGG CTCCGTACAG 
CAGATGGAAC AGCTGAACCG CCGGTACCAG AATATCCAGC GGACTATTGC TACCGAGAGA 
TCGGAAGACG AGCCACCCAG TCAGAGTGCG GAGCCTCTCC CTGCCGGAGG AGGGAGTGGA 
GGAGGGGGAG GAGGGAGTGA CTTGGAAGCA GACTCTTCTC AACCAAATCT AGAACTGAGG 
AGAGAGGGGT CTCTTTCTCC TGTGAACTCC CAAAAAATCA CTTTGCTGCT TCAGTCCCCG 
GCCGTCAAGT TCATCACCAA CCCTGAGTTC TTCACCGTGC TGCATGCCAA CTATAGTGCC 
TATAGAGTCT TCACCAACAG CACCTGCTTG AAGCATATGA TTCTGAAGAT CCGCAGGGAT 
GCCCGGAATT TCGAACGCTA CCAGCACAAC CGGGACCTGG TGAACTTCAT CAACATGTTT 
GCTGACACCC GTTTGGAACT GCCTCGCGGC TGGGAAATCA AAACTGACCA GCAGGGCAAG 
TCCTTCTTCG TCGACCACAA CAGTCGAGCC ACCACTTTCA TCGACCCCCG GATCCCTCTT 
CAGAACGGAC GTCTTCCGAA TCACCTGACT CACCGGCAGC ACCTTCAGCG ACTCCGGAGC 
TACAGTGCCG GCGAGGCCTC AGAAGTCTCT CGGAACCGAG GAGCTTCTCT GTTGGCCAGG 
CCGGGTCACA GCCTGGTAGC AGCCATCAGA AACCAACATC ACCATGAGAC GTTACCTCTG 
GCTTATAATG ACAAGATCGT GGCATTTCTC CGCCAGCCAA ACATTTTTGA GATGCTGCAG 
GAGCGCCAGC CCAGCTTGGC CAGAAACCAC GCACTCAGGG AGAAAATCCA TTACATTCGG 
ACAGAAGGTA CTCAAGGGCT TGAAAAATTG TCCTGTGATG CAGATTTAGT AATACTGCTG 
AGCCTCTTTG AAGAAGAGAT CATGTCCTAC ATACCCCTTC AGGCCGCTTT CCACCCGGGA 
TACAGCTTCT CTCCTCGCTG TTCACCGTGC TCTTCCCCTC AAAACTCTCC AGGCCTACAG 
CGAGCCAGTG TCAGGGCCCC CTCACCCTAC AGGAGAGACT TTGAGGCCAA GCTCCGAAAT 
TTCTACCGAA AACTTGAAGC CAAAGGATTT GGTCAAGGTC CGGGTAAAAT CAAACTCATC 
ATAAGACGTG ACCACTTGCT GGAGGGAACC TTCAATCAGG TGATGGCTTA TTCCCGCAAA 
GAGCTCCAGC GGAACAAGCT CTACGTCACA TTTGTTGGAG AGGAGGGCCT AGACTACAGT 
GGGCCCTCCC GGGAGTTCTT TTTCCTTTTG TCCCAGGAGC TCTTCAACCC CTACTACGGC 
CTGTTTGAGT ATTCTGCCAA TGACACTTAC ACCGTGCAGA TCAGCCCCAT GTCGGCCTTT 
GTGGAAAATC ATCTCGAGTG GTTCAGATTT AGCGGTCGCA TCCTTGGGCT TGCCCTGATC 
CACCAGTACC TCCTGGACGC TTTCTTCACG AGGCCGTTCT ACAAGGCCCT GCTGAGACTG 
CCCTGTGATC TGAGCGACCT AGAGTATCTG GATGAGGAAT TCCACCAAAG CCTGCAGTGG 
ATGAAGGACA ATAACATCAC GGATATCTTA GATCTCACTT TCACAGTGAA TGAAGAAGTG 
TTTGGACAGG TAACAGAAAG AGAGTTGAAA TCAGGGGGAG CCAACACCCA GGTGACAGAA 
AAAAATAAAA AGGAGTACAT TGAGAGAATG GTGAAGTGGA GAGTGGAGCG GGGCGTGGTG 
CAGCAGACGG AAGCGTTAGT CCGAGGCTTC TATGAAGTTG TGGATTCAAG GCTCGTCTCC 
GTCTTTGATG CCAGGGAGCT GGAGCTGGTG ATCGCCGGCA CCGCCGAAAT AGACCTCAAT 
GACTGGCGCA ATAACACCGA GTATCGAGGG GGTTATCACG ATGGTCATAT CGTGATCCGG 
TGGTTCTGGG CAGCTGTGGA GAGGTTCAAC AATGAGCAGA GACTGAGGTT GCTTCAGTTC 
GTCACGGGAA CATCGAGCGT ACCCTATGAA GGTTTCGCCG CGCTCCGAGG AAGCAATGGG 
CTGAGGCGCT TCTGTATAGA GAAATGGGGG AAAATAACAT CCCTTCCCAG GGCACACACG 
TGTTTCAACC GCTTGGATCT GCCACCGTAC CCATCGTATT CCATGCTGTA CGAGAAGCTG 
TTGACAGCAG TTGAGGAAAC GAGCACCTTT GGTCTGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_029063057.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028918890.1 Ornithorhynchus anatinus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X6, mRNA.

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CloneID	OOc127717
Clone ID Related Accession (Same CDS sequence)	XM_029063057.1
Accession Version	XM_029063057.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	E3 ubiquitin-protein ligase HECW1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041731.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCGC CGTCGAGGAA CTCCCAGAGC CGGCGCCGCT GTAAAGAGCC CCTGCGCTAC 
AGCTACAACC CCGACCAGTT CCACAACATG GACATCAGGA ACGGCCCACA CGGGACCGTC 
ACCATCCCCC GCTCCACCAG CGACACCGAC CTGGTCACGT CCGACAGTCG GTCCACCCTT 
ATGGTCAGCA GTTCGTATTA CTCCATTGGA CACTCTCAGG ACCTGGTGAT CTATTGGGAT 
ATAAAAGAGG AAGTGGGAGC CGGGGACTGG ATTGGCATGT ATCTCATCGA TGAGGTCTTA 
TCTGAAAATT TTCTGGACTA CAAAAACCGC GGAGTGAATG GTTCTCACCG GGGCCAGATT 
ATCTGGAAGA TTGATGCAAG TTCTTACTTT GTGGAATCTG AAACTAAGAT CTGCTTCAAA 
TACTATCATG GAGTGAGTGG AGCGCTACGA GCCACTACGC CCAGTGTTAC AGTGAAAAAC 
TCTTCAGCTC CTGTTTTTAA GAGCATCACC AATGACGAGG CCACCCAGGG TCAAGGCAGT 
CGGAGGCTGA TCAGTTTTTC TCTCTCAGAT TTCCAGGCCA TGGGTCTGAA GAAAGGAATG 
TTCTTTAACC CAGATCCATA TCTGAAGATT TCCATCCAAC CTGGGAAACA CAGCATCTTC 
CCAGCCCTTC CGCACCATGG TCAGGAGAAG AGGTCGAAGA TTACCTGTAA CACCGTGAAC 
CCTGTTTGGC AAGCTGAGCA GTTTAGCTTT GTTTCCCTGC CCACCGACGT CCTAGAAATC 
GAGGTGAAAG ACAAGTTTGC CAAGAGTCGT CCAATCATCA AACGCTTCTT AGGAAAGTTG 
TCCATGCCTG TTCAGCGACT CCTGGAGAGA CACGCTATAG GGGACAGGGT GGTGAGCTAC 
ACTCTGGGTC GAAGGCTTCC AACGGATCAC GTCAGCGGCC AGCTCCAGTT CCGGTTCGAG 
ATCACCTCCT CCATCCACCC AGATGACGAG GAGGTCTCTC TGAGCGCAGA TCCCGAGTCT 
GCTGAAAACC AGGAGAGTCC TGCCAACCAC CCATCAGAGG AAGGCCTCAC AGAACCTCCA 
GGCCAGGGGA CGGAGGGCCC AGAGGGCCCG GAGCTGGAGA CACAGGATGG TGGTGGTCCC 
ACGAGTGGCT CTGGGGGAGA CAGCCCCGAG GCTACCGGAC CGGCCGGGGA AAAGTCGAAT 
GAGGAGGAGG CCGCAGAGGG TGCCGAAGCC GTCGTGGAGC CAGTTGATGC CGTCGATCCC 
ACGGAACCCA GCCCGGCCGA AGATTTGCCT TCCCTCAGTG CTGAAGAGAT CGCCGAGGGG 
CTGGATCTGC TCCCTGGCAC TCCCCCGCCA CCGCCTCCGG AAGACGAGGG TTGGGAGCAG 
GGGGAGAACC ACGCCGGAGA GGCCGATGGA GATCCCCTTG TCGGCCTGGA GGAGCCAGAG 
GGGGCTGAGG CAGAGGTGGA GAAAAAGGTT GACGTGAGCA TGGTTCTAGA CAAAGACCAG 
GATGAAGAGG AAGAAGAATT GGCCTCCCCC GAAGAGGATG GGCCTCTGGC GCTGAGACCC 
TCGGCCAAGA AGAAAAGCAG ACCGTGCTCC CTGCCCGTGT CCGAGTTGGA GACGGTGATC 
GCGTCGGCCT GCGGCGAACC GGAAACGCCC CGCACCCATT ACATCCGCAT CCACAACCTG 
CTGCACAGCA TGCCCTCAGC CCAGGGAGGC AGCGATGGCG GAGAAGCCCA AGGACCGGAG 
GAAGAGGACG AGGAGGGGCC GGGGGAGGAA GAGTCAACGG TCAAGGAGGC CTCCGAAAAG 
GACGTGGTCA GCGAGGCCGA GACCGTGGCA CCGGACCCAC CTCACCCAGG AGATGGCGGA 
GAGAGCGGGG ACCCCGAGGT ATCTGGCCAG CGGCCGGTGC CCCACAGTGC CTCGGCCGAG 
TGCTTGGCCG GGGTGAACGG TGGCCCGCTT CTCGACGGCG AGCCCCGGCC CAGCACGGGG 
AGCGAGAGCG ACTCGAGCCC CCGGCAGGAC TGCGAGGGCT GCGACACATC CTGCTACAGC 
CCGTCCTGCT ACAGCACCTC GTGTTACAGC ACCTCCTGCT ACAGCACCTC GTGCTACAGC 
ACCTCCTGCT ACAGCCCATC CTGCTACGAC AGCAACAACC GGTTCTCCAG CCATACGCGC 
TTCTCCTCGG TGGACAGTGC CAAGATCTCG GAGAGCACCG TCTTCTCCTC TCAGGACGAG 
GAGGAGGAGG AGAACAGCGC GTTCGAGTCG GTGCCCGACT CGGTGCAGAG CCCGGAGATG 
GACCGGGAGA CGGCCGACGG CACCGGCCCG TGGCCGGACG AGCTGGTTCT CCCCAACGGG 
AACGTTCCGA GGACAGCCGA TGGCCTGGAG TCCCCGATAG CTGGTCCCAG TAATCGGAGA 
GAAGCTGAAG TACTACATTT TCATCATATC ATTTCGTCGG CTTGGATTTC ACTAGCAGGT 
GATTGTCCTT TACTACATAA TTCTCAGCCA GTCAGCCAGC TTCCTTCCTT GAGGCCTGAC 
CACCACCATT ATCCAACTAT CGATGAGCCT CTTCCGCCAA ACTGGGAGGC TCGAATAGAC 
AGTCACGGGA GAGTGTTTTA CGTGGACCAC GTGAACCGAA CGACGACGTG GCAGCGGCCT 
ACCACGGCCG CCACTCCAGA CGGGATCCGC AGATCTGGCT CCGTACAGCA GATGGAACAG 
CTGAACCGCC GGTACCAGAA TATCCAGCGG ACTATTGCTA CCGAGAGATC GGAAGACGAG 
CCACCCAGTC AGAGTGCGGA GCCTCTCCCT GCCGGAGGAG GGAGTGGAGG AGGGGGAGGA 
GGGAGTGACT TGGAAGCAGA CTCTTCTCAA CCAAATCTAG AACTGAGGAG AGAGGGGTCT 
CTTTCTCCTG TGAACTCCCA AAAAATCACT TTGCTGCTTC AGTCCCCGGC CGTCAAGTTC 
ATCACCAACC CTGAGTTCTT CACCGTGCTG CATGCCAACT ATAGTGCCTA TAGAGTCTTC 
ACCAACAGCA CCTGCTTGAA GCATATGATT CTGAAGATCC GCAGGGATGC CCGGAATTTC 
GAACGCTACC AGCACAACCG GGACCTGGTG AACTTCATCA ACATGTTTGC TGACACCCGT 
TTGGAACTGC CTCGCGGCTG GGAAATCAAA ACTGACCAGC AGGGCAAGTC CTTCTTCGTC 
GACCACAACA GTCGAGCCAC CACTTTCATC GACCCCCGGA TCCCTCTTCA GAACGGACGT 
CTTCCGAATC ACCTGACTCA CCGGCAGCAC CTTCAGCGAC TCCGGAGCTA CAGTGCCGGC 
GAGGCCTCAG AAGTCTCTCG GAACCGAGGA GCTTCTCTGT TGGCCAGGCC GGGTCACAGC 
CTGGTAGCAG CCATCAGAAA CCAACATCAC CATGAGACGT TACCTCTGGC TTATAATGAC 
AAGATCGTGG CATTTCTCCG CCAGCCAAAC ATTTTTGAGA TGCTGCAGGA GCGCCAGCCC 
AGCTTGGCCA GAAACCACGC ACTCAGGGAG AAAATCCATT ACATTCGGAC AGAAGGTACT 
CAAGGGCTTG AAAAATTGTC CTGTGATGCA GATTTAGTAA TACTGCTGAG CCTCTTTGAA 
GAAGAGATCA TGTCCTACAT ACCCCTTCAG GCCGCTTTCC ACCCGGGATA CAGCTTCTCT 
CCTCGCTGTT CACCGTGCTC TTCCCCTCAA AACTCTCCAG GCCTACAGCG AGCCAGTGTC 
AGGGCCCCCT CACCCTACAG GAGAGACTTT GAGGCCAAGC TCCGAAATTT CTACCGAAAA 
CTTGAAGCCA AAGGATTTGG TCAAGGTCCG GGTAAAATCA AACTCATCAT AAGACGTGAC 
CACTTGCTGG AGGGAACCTT CAATCAGGTG ATGGCTTATT CCCGCAAAGA GCTCCAGCGG 
AACAAGCTCT ACGTCACATT TGTTGGAGAG GAGGGCCTAG ACTACAGTGG GCCCTCCCGG 
GAGTTCTTTT TCCTTTTGTC CCAGGAGCTC TTCAACCCCT ACTACGGCCT GTTTGAGTAT 
TCTGCCAATG ACACTTACAC CGTGCAGATC AGCCCCATGT CGGCCTTTGT GGAAAATCAT 
CTCGAGTGGT TCAGATTTAG CGGTCGCATC CTTGGGCTTG CCCTGATCCA CCAGTACCTC 
CTGGACGCTT TCTTCACGAG GCCGTTCTAC AAGGCCCTGC TGAGACTGCC CTGTGATCTG 
AGCGACCTAG AGTATCTGGA TGAGGAATTC CACCAAAGCC TGCAGTGGAT GAAGGACAAT 
AACATCACGG ATATCTTAGA TCTCACTTTC ACAGTGAATG AAGAAGTGTT TGGACAGGTA 
ACAGAAAGAG AGTTGAAATC AGGGGGAGCC AACACCCAGG TGACAGAAAA AAATAAAAAG 
GAGTACATTG AGAGAATGGT GAAGTGGAGA GTGGAGCGGG GCGTGGTGCA GCAGACGGAA 
GCGTTAGTCC GAGGCTTCTA TGAAGTTGTG GATTCAAGGC TCGTCTCCGT CTTTGATGCC 
AGGGAGCTGG AGCTGGTGAT CGCCGGCACC GCCGAAATAG ACCTCAATGA CTGGCGCAAT 
AACACCGAGT ATCGAGGGGG TTATCACGAT GGTCATATCG TGATCCGGTG GTTCTGGGCA 
GCTGTGGAGA GGTTCAACAA TGAGCAGAGA CTGAGGTTGC TTCAGTTCGT CACGGGAACA 
TCGAGCGTAC CCTATGAAGG TTTCGCCGCG CTCCGAGGAA GCAATGGGCT GAGGCGCTTC 
TGTATAGAGA AATGGGGGAA AATAACATCC CTTCCCAGGG CACACACGTG TTTCAACCGC 
TTGGATCTGC CACCGTACCC ATCGTATTCC ATGCTGTACG AGAAGCTGTT GACAGCAGTT 
GAGGAAACGA GCACCTTTGG TCTGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028918890.1
CDS	141..5030
Translation	MASPSRNSQSRRRCKEPLRYSYNPDQFHNMDIRNGPHGTVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIYWDIKEEVGAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSSAPVFKSITNDEATQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQEKRSKITCNTVNPVWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEVSLSADPESAENQESPANHPSEEGLTEPPGQGTEGPEGPELETQDGGGPTSGSGGDSPEATGPAGEKSNEEEAAEGAEAVVEPVDAVDPTEPSPAEDLPSLSAEEIAEGLDLLPGTPPPPPPEDEGWEQGENHAGEADGDPLVGLEEPEGAEAEVEKKVDVSMVLDKDQDEEEEELASPEEDGPLALRPSAKKKSRPCSLPVSELETVIASACGEPETPRTHYIRIHNLLHSMPSAQGGSDGGEAQGPEEEDEEGPGEEESTVKEASEKDVVSEAETVAPDPPHPGDGGESGDPEVSGQRPVPHSASAECLAGVNGGPLLDGEPRPSTGSESDSSPRQDCEGCDTSCYSPSCYSTSCYSTSCYSTSCYSTSCYSPSCYDSNNRFSSHTRFSSVDSAKISESTVFSSQDEEEEENSAFESVPDSVQSPEMDRETADGTGPWPDELVLPNGNVPRTADGLESPIAGPSNRREAEVLHFHHIISSAWISLAGDCPLLHNSQPVSQLPSLRPDHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTTAATPDGIRRSGSVQQMEQLNRRYQNIQRTIATERSEDEPPSQSAEPLPAGGGSGGGGGGSDLEADSSQPNLELRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTNSTCLKHMILKIRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRNQHHHETLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHALREKIHYIRTEGTQGLEKLSCDADLVILLSLFEEEIMSYIPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASVRAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_029063057.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029063057.1

ORF Insert Sequence:

ATGGCCTCGC CGTCGAGGAA CTCCCAGAGC CGGCGCCGCT GTAAAGAGCC CCTGCGCTAC 
AGCTACAACC CCGACCAGTT CCACAACATG GACATCAGGA ACGGCCCACA CGGGACCGTC 
ACCATCCCCC GCTCCACCAG CGACACCGAC CTGGTCACGT CCGACAGTCG GTCCACCCTT 
ATGGTCAGCA GTTCGTATTA CTCCATTGGA CACTCTCAGG ACCTGGTGAT CTATTGGGAT 
ATAAAAGAGG AAGTGGGAGC CGGGGACTGG ATTGGCATGT ATCTCATCGA TGAGGTCTTA 
TCTGAAAATT TTCTGGACTA CAAAAACCGC GGAGTGAATG GTTCTCACCG GGGCCAGATT 
ATCTGGAAGA TTGATGCAAG TTCTTACTTT GTGGAATCTG AAACTAAGAT CTGCTTCAAA 
TACTATCATG GAGTGAGTGG AGCGCTACGA GCCACTACGC CCAGTGTTAC AGTGAAAAAC 
TCTTCAGCTC CTGTTTTTAA GAGCATCACC AATGACGAGG CCACCCAGGG TCAAGGCAGT 
CGGAGGCTGA TCAGTTTTTC TCTCTCAGAT TTCCAGGCCA TGGGTCTGAA GAAAGGAATG 
TTCTTTAACC CAGATCCATA TCTGAAGATT TCCATCCAAC CTGGGAAACA CAGCATCTTC 
CCAGCCCTTC CGCACCATGG TCAGGAGAAG AGGTCGAAGA TTACCTGTAA CACCGTGAAC 
CCTGTTTGGC AAGCTGAGCA GTTTAGCTTT GTTTCCCTGC CCACCGACGT CCTAGAAATC 
GAGGTGAAAG ACAAGTTTGC CAAGAGTCGT CCAATCATCA AACGCTTCTT AGGAAAGTTG 
TCCATGCCTG TTCAGCGACT CCTGGAGAGA CACGCTATAG GGGACAGGGT GGTGAGCTAC 
ACTCTGGGTC GAAGGCTTCC AACGGATCAC GTCAGCGGCC AGCTCCAGTT CCGGTTCGAG 
ATCACCTCCT CCATCCACCC AGATGACGAG GAGGTCTCTC TGAGCGCAGA TCCCGAGTCT 
GCTGAAAACC AGGAGAGTCC TGCCAACCAC CCATCAGAGG AAGGCCTCAC AGAACCTCCA 
GGCCAGGGGA CGGAGGGCCC AGAGGGCCCG GAGCTGGAGA CACAGGATGG TGGTGGTCCC 
ACGAGTGGCT CTGGGGGAGA CAGCCCCGAG GCTACCGGAC CGGCCGGGGA AAAGTCGAAT 
GAGGAGGAGG CCGCAGAGGG TGCCGAAGCC GTCGTGGAGC CAGTTGATGC CGTCGATCCC 
ACGGAACCCA GCCCGGCCGA AGATTTGCCT TCCCTCAGTG CTGAAGAGAT CGCCGAGGGG 
CTGGATCTGC TCCCTGGCAC TCCCCCGCCA CCGCCTCCGG AAGACGAGGG TTGGGAGCAG 
GGGGAGAACC ACGCCGGAGA GGCCGATGGA GATCCCCTTG TCGGCCTGGA GGAGCCAGAG 
GGGGCTGAGG CAGAGGTGGA GAAAAAGGTT GACGTGAGCA TGGTTCTAGA CAAAGACCAG 
GATGAAGAGG AAGAAGAATT GGCCTCCCCC GAAGAGGATG GGCCTCTGGC GCTGAGACCC 
TCGGCCAAGA AGAAAAGCAG ACCGTGCTCC CTGCCCGTGT CCGAGTTGGA GACGGTGATC 
GCGTCGGCCT GCGGCGAACC GGAAACGCCC CGCACCCATT ACATCCGCAT CCACAACCTG 
CTGCACAGCA TGCCCTCAGC CCAGGGAGGC AGCGATGGCG GAGAAGCCCA AGGACCGGAG 
GAAGAGGACG AGGAGGGGCC GGGGGAGGAA GAGTCAACGG TCAAGGAGGC CTCCGAAAAG 
GACGTGGTCA GCGAGGCCGA GACCGTGGCA CCGGACCCAC CTCACCCAGG AGATGGCGGA 
GAGAGCGGGG ACCCCGAGGT ATCTGGCCAG CGGCCGGTGC CCCACAGTGC CTCGGCCGAG 
TGCTTGGCCG GGGTGAACGG TGGCCCGCTT CTCGACGGCG AGCCCCGGCC CAGCACGGGG 
AGCGAGAGCG ACTCGAGCCC CCGGCAGGAC TGCGAGGGCT GCGACACATC CTGCTACAGC 
CCGTCCTGCT ACAGCACCTC GTGTTACAGC ACCTCCTGCT ACAGCACCTC GTGCTACAGC 
ACCTCCTGCT ACAGCCCATC CTGCTACGAC AGCAACAACC GGTTCTCCAG CCATACGCGC 
TTCTCCTCGG TGGACAGTGC CAAGATCTCG GAGAGCACCG TCTTCTCCTC TCAGGACGAG 
GAGGAGGAGG AGAACAGCGC GTTCGAGTCG GTGCCCGACT CGGTGCAGAG CCCGGAGATG 
GACCGGGAGA CGGCCGACGG CACCGGCCCG TGGCCGGACG AGCTGGTTCT CCCCAACGGG 
AACGTTCCGA GGACAGCCGA TGGCCTGGAG TCCCCGATAG CTGGTCCCAG TAATCGGAGA 
GAAGCTGAAG TACTACATTT TCATCATATC ATTTCGTCGG CTTGGATTTC ACTAGCAGGT 
GATTGTCCTT TACTACATAA TTCTCAGCCA GTCAGCCAGC TTCCTTCCTT GAGGCCTGAC 
CACCACCATT ATCCAACTAT CGATGAGCCT CTTCCGCCAA ACTGGGAGGC TCGAATAGAC 
AGTCACGGGA GAGTGTTTTA CGTGGACCAC GTGAACCGAA CGACGACGTG GCAGCGGCCT 
ACCACGGCCG CCACTCCAGA CGGGATCCGC AGATCTGGCT CCGTACAGCA GATGGAACAG 
CTGAACCGCC GGTACCAGAA TATCCAGCGG ACTATTGCTA CCGAGAGATC GGAAGACGAG 
CCACCCAGTC AGAGTGCGGA GCCTCTCCCT GCCGGAGGAG GGAGTGGAGG AGGGGGAGGA 
GGGAGTGACT TGGAAGCAGA CTCTTCTCAA CCAAATCTAG AACTGAGGAG AGAGGGGTCT 
CTTTCTCCTG TGAACTCCCA AAAAATCACT TTGCTGCTTC AGTCCCCGGC CGTCAAGTTC 
ATCACCAACC CTGAGTTCTT CACCGTGCTG CATGCCAACT ATAGTGCCTA TAGAGTCTTC 
ACCAACAGCA CCTGCTTGAA GCATATGATT CTGAAGATCC GCAGGGATGC CCGGAATTTC 
GAACGCTACC AGCACAACCG GGACCTGGTG AACTTCATCA ACATGTTTGC TGACACCCGT 
TTGGAACTGC CTCGCGGCTG GGAAATCAAA ACTGACCAGC AGGGCAAGTC CTTCTTCGTC 
GACCACAACA GTCGAGCCAC CACTTTCATC GACCCCCGGA TCCCTCTTCA GAACGGACGT 
CTTCCGAATC ACCTGACTCA CCGGCAGCAC CTTCAGCGAC TCCGGAGCTA CAGTGCCGGC 
GAGGCCTCAG AAGTCTCTCG GAACCGAGGA GCTTCTCTGT TGGCCAGGCC GGGTCACAGC 
CTGGTAGCAG CCATCAGAAA CCAACATCAC CATGAGACGT TACCTCTGGC TTATAATGAC 
AAGATCGTGG CATTTCTCCG CCAGCCAAAC ATTTTTGAGA TGCTGCAGGA GCGCCAGCCC 
AGCTTGGCCA GAAACCACGC ACTCAGGGAG AAAATCCATT ACATTCGGAC AGAAGGTACT 
CAAGGGCTTG AAAAATTGTC CTGTGATGCA GATTTAGTAA TACTGCTGAG CCTCTTTGAA 
GAAGAGATCA TGTCCTACAT ACCCCTTCAG GCCGCTTTCC ACCCGGGATA CAGCTTCTCT 
CCTCGCTGTT CACCGTGCTC TTCCCCTCAA AACTCTCCAG GCCTACAGCG AGCCAGTGTC 
AGGGCCCCCT CACCCTACAG GAGAGACTTT GAGGCCAAGC TCCGAAATTT CTACCGAAAA 
CTTGAAGCCA AAGGATTTGG TCAAGGTCCG GGTAAAATCA AACTCATCAT AAGACGTGAC 
CACTTGCTGG AGGGAACCTT CAATCAGGTG ATGGCTTATT CCCGCAAAGA GCTCCAGCGG 
AACAAGCTCT ACGTCACATT TGTTGGAGAG GAGGGCCTAG ACTACAGTGG GCCCTCCCGG 
GAGTTCTTTT TCCTTTTGTC CCAGGAGCTC TTCAACCCCT ACTACGGCCT GTTTGAGTAT 
TCTGCCAATG ACACTTACAC CGTGCAGATC AGCCCCATGT CGGCCTTTGT GGAAAATCAT 
CTCGAGTGGT TCAGATTTAG CGGTCGCATC CTTGGGCTTG CCCTGATCCA CCAGTACCTC 
CTGGACGCTT TCTTCACGAG GCCGTTCTAC AAGGCCCTGC TGAGACTGCC CTGTGATCTG 
AGCGACCTAG AGTATCTGGA TGAGGAATTC CACCAAAGCC TGCAGTGGAT GAAGGACAAT 
AACATCACGG ATATCTTAGA TCTCACTTTC ACAGTGAATG AAGAAGTGTT TGGACAGGTA 
ACAGAAAGAG AGTTGAAATC AGGGGGAGCC AACACCCAGG TGACAGAAAA AAATAAAAAG 
GAGTACATTG AGAGAATGGT GAAGTGGAGA GTGGAGCGGG GCGTGGTGCA GCAGACGGAA 
GCGTTAGTCC GAGGCTTCTA TGAAGTTGTG GATTCAAGGC TCGTCTCCGT CTTTGATGCC 
AGGGAGCTGG AGCTGGTGAT CGCCGGCACC GCCGAAATAG ACCTCAATGA CTGGCGCAAT 
AACACCGAGT ATCGAGGGGG TTATCACGAT GGTCATATCG TGATCCGGTG GTTCTGGGCA 
GCTGTGGAGA GGTTCAACAA TGAGCAGAGA CTGAGGTTGC TTCAGTTCGT CACGGGAACA 
TCGAGCGTAC CCTATGAAGG TTTCGCCGCG CTCCGAGGAA GCAATGGGCT GAGGCGCTTC 
TGTATAGAGA AATGGGGGAA AATAACATCC CTTCCCAGGG CACACACGTG TTTCAACCGC 
TTGGATCTGC CACCGTACCC ATCGTATTCC ATGCTGTACG AGAAGCTGTT GACAGCAGTT 
GAGGAAACGA GCACCTTTGG TCTGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_007663451.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_007661641.1 Ornithorhynchus anatinus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), mRNA.

Price & Availability↑

CloneID	OOc15631
Clone ID Related Accession (Same CDS sequence)	XM_007663451.1
Accession Version	XM_007663451.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-14
Organism	Ornithorhynchus anatinus(platypus)
Product	E3 ubiquitin-protein ligase HECW1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001755598.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ornithorhynchus anatinus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGTG CCCAGCCTGG CATCATTCAG AATCTGTACC AGAACAGGTT CCTGGGTCTG 
GCCGCCATGG CCTCGCCGTC GAGGAACTCC CAGAGCCGGC GCCGCTGTAA AGAGCCCCTG 
CGCTACAGCT ACAACCCCGA CCAGTTCCAC AACATGGACA TCAGGAACGG CCCACACGGG 
ACCGTCACCA TCCCCCGCTC CACCAGCGAC ACCGACCTGG TCACGTCCGA CAGTCGGTCC 
ACCCTTATGG TCAGCAGTTC GTATTACTCC ATTGGACACT CTCAGGACCT GGTGATCTAT 
TGGGATATAA AAGAGGAAGT GGGAGCCGGG GACTGGATTG GCATGTATCT CATCGATGAG 
GTCCTATCTG AAAATTTTCT GGACTACAAA AACCGCGGAG TGAATGGTTC TCACCGGGGC 
CAGATTATCT GGAAGATTGA TGCAAGTTCT TACTTTGTGG AATCTGAAAC TAAGATCTGC 
TTCAAATACT ATCATGGAGT GAGTGGAGCG CTACGAGCCA CTACGCCCAG TGTTACAGTG 
AAAAACTCTT CAGCTCCTGT TTTTAAGAGC ATCACCAATG ACGAGGCCAC CCAGGGTCAA 
GGCAGTCGGA GGCTGATCAG TTTTTCTCTC TCAGATTTCC AGGCCATGGG TCTGAAGAAA 
GGAATGTTCT TTAACCCAGA TCCATATCTG AAGATTTCCA TCCAACCTGG GAAACACAGC 
ATCTTCCCAG CCCTTCCGCA CCATGGTCAG GAGAAGAGGT CGAAGATTAC CTGTAACACC 
GTGAACCCTG TTTGGCAAGC TGAGCAGTTT AGCTTTGTTT CCCTGCCCAC CGACGTCCTA 
GAAATCGAGG TGAAAGACAA GTTTGCCAAG AGTCGTCCAA TCATCAAACG CTTCTTAGGA 
AAGTTGTCCA TGCCTGTTCA GCGACTCCTG GAGAGACACG CTATAGGGGA CAGGGTGGTG 
AGCTACACTC TGGGTCGAAG GCTTCCAACG GATCACGTCA GCGGCCAGCT CCAGTTCCGG 
TTCGAGATCA CCTCCTCCAT CCACCCAGAT GACGAGGAGG TCTCTCTGAG CGCAGATCCC 
GAGTCTGCTG AAAACCAGGA GAGTCCTGCC AACCACCCAT CAGAGGAAGG CCTCACAGAA 
CCTCCAGGCC AGGGGACGGA GGGCCCAGAG GGCCCGGAGC TGGAGACACA GGATGGTGGT 
GGTCCCACGA GTGGCTCTGG GGGAGACAGC CCCGAGGCTA CCGGACCGGC CGGGGAAAAG 
TCGAATGAGG AGGAGGCCGC AGAGGGTGCC GAAGCCGTCG TGGAGCCAGT TGATGCCGTC 
GATCCCACGG AACCCAGCCC GGCCGAAGAT TTGCCTTCCC TCAGTGCTGA AGAGATCGCC 
GAGGGGCTGG ATCTGCTCCC TGGCACTCCC CCGCCACCGC CTCCGGAAGA CGAGGGTTGG 
GAGCAGGGGG AGAACCACGC CGGAGAGGCC GATGGAGATC CCCTTGTCGG CCTGGAGGAG 
CCAGAGGGGG CTGAGGCAGA GGTGGAGAAA AAGGTTGACG TGAGCATGGT TCTAGACAAA 
GACCAGGATG AAGAGGAAGA AGAATTGGCC TCCCCCGAAG AGGATGGGCC TCTGGCGCTG 
AGACCCTCGG CCAAGAAGAA AAGCAGACCG TGCTCCCTGC CCGTGTCCGA GTTGGAGACG 
GTGATCGCGT CGGCCTGCGG CGAACCGGAA ACGCCCCGCA CCCATTACAT CCGCATCCAC 
AACCTGCTGC ACAGCATGCC CTCAGCCCAG GGAGGCAGCG ATGGCGGAGA AGCCCAAGGA 
CCGGAGGAAG AGGACGAGGA GGGGCCGGGG GAGGAAGAGT CAACGGTCAA GGAGGCCTCC 
GAAAAGGACG TGGTCAGCGA GGCCGAGACC GTGGCACCGG ACCCACCTCA CCCAGAAGAT 
GGCGGAGAGA GCGGGGACCC CGAGGTATCT GGCCAGCGGC CGGTGCCCCA CAGTGCCTCG 
GCCGAGTGCT TGGCCGGGGT GAACGGTGGC CCGCTTCTCG ACGGCGAGCC CCGGCCCAGC 
ACGGGGAGCG AGAGCGACTC GAGCCCCCGG CAGGACTGCG AGGGCTGCGA CACATCCTGC 
TACAGCCCGT CCTGCTACAG CACCTCGTGT TACAGCACCT CCTGCTACAG CACCTCGTGC 
TACAGCACCT CCTGCTACAG CCCATCCTGC TACGACAGCA ACAACCGGTT CTCCAGCCAT 
ACGCGCTTCT CCTCGGTGGA CAGTGCCAAG ATCTCGGAGA GCACCGTCTT CTCCTCTCAG 
GACGAGGAGG AGGAGGAGAA CAGCGCGTTC GAGTCGGTGC CCGACTCGGT GCAGAGCCCG 
GAGATGGACC GGGAGACGGC CGACGGCACC GGCCCGTGGC CGGACGAGCT GGTTCTCCCC 
AACGGGAACG TTCCGAGGAC AGCCGATGGC CTGGAGTCCC CGATAGCTGG TCCCAGTAAT 
CGGAGAGAAG GTGATTGTCC TTTACTACAT AATTCTCAGC CAGTCAGCCA GCTTCCTTCC 
TTGAGGCCTG ACCACCACCA TTATCCAACT ATCGATGAGC CTCTTCCGCC AAACTGGGAG 
GCTCGAATAG ACAGTCACGG GAGAGTGTTT TACGTGGACC ACGTGAACCG AACGACGACG 
TGGCAGCGGC CTACCACGGC CGCCACTCCA GACGGGATCC GCAGATCTGG CTCCGTACAG 
CAGATGGAAC AGCTGAACCG CCGGTACCAG AATATCCAGC GGACTATTGC TACCGAGAGA 
TCGGAAGACG AGCCACCCAG TCAGAGTGCG GAGCCTCTCC CTGCCGGAGG AGGGAGTGGA 
GGAGGGGGAG GAGGGAGTGA CTTGGAAGCA GACTCTTCTC AACCAAATCT AGAACTGAGG 
AGAGAGGGGT CTCTTTCTCC TGTGAACTCC CAAAAAATCA CTTTGCTGCT TCAGTCCCCG 
GCCGTCAAGT TCATCACCAA CCCTGAGTTC TTCACCGTGC TGCATGCCAA CTATAGTGCC 
TATAGAGTCT TCACCAACAG CACCTGCTTG AAGCATATGA TTCTGAAGAT CCGCAGGGAT 
GCCCGGAATT TCGAACGCTA CCAGCACAAC CGGGACCTGG TGAACTTCAT CAACATGTTT 
GCTGACACCC GTTTGGAACT GCCTCGCGGC TGGGAAATCA AAACTGACCA GCAGGGCAAG 
TCCTTCTTCG TCGACCACAA CAGTCGAGCC ACCACTTTCA TCGACCCCCG GATCCCTCTT 
CAGAACGGAC GTCTTCCGAA TCACCTGACT CACCGGCAGC ACCTTCAGCG ACTCCGGAGC 
TACAGTGCCG GCGAGGCCTC AGAAGTCTCT CGGAACCGAG GAGCTTCTCT GTTGGCCAGG 
CCGGGTCACA GCCTGGTAGC AGCCATCAGA AACCAACATC ACCATGAGAC GTTACCTCTG 
GCTTATAATG ACAAGATCGT GGCATTTCTC CGCCAGCCAA ACATTTTTGA GATGCTGCAG 
GAGCGCCAGC CCAGCTTGGC CAGAAACCAC GCACTCAGGG AGAAAATCCA TTACATTCGG 
ACAGAAGGTA CTCAAGGGCT TGAAAAATTG TCCTGTGATG CAGATTTAGT AATACTGCTG 
AGCCTCTTTG AAGAAGAGAT CATGTCCTAC ATACCCCTTC AGGCCGCTTT CCACCCGGGA 
TACAGCTTCT CTCCTCGCTG TTCACCGTGC TCTTCCCCTC AAAACTCTCC AGGCCTACAG 
CGAGCCAGTG TCAGGGCCCC CTCACCCTAC AGGAGAGACT TTGAGGCCAA GCTCCGAAAT 
TTCTACCGAA AACTTGAAGC CAAAGGATTT GGTCAAGGTC CGGGTAAAAT CAAACTCATC 
ATAAGACGTG ACCACTTGCT GGAGGGAACC TTCAATCAGG TGATGGCTTA TTCCCGCAAA 
GAGCTCCAGC GGAACAAGCT CTACGTCACA TTTGTTGGAG AGGAGGGCCT AGACTACAGT 
GGGCCCTCCC GGGAGTTCTT TTTCCTTTTG TCCCAGGAGC TCTTCAACCC CTACTACGGC 
CTGTTTGAGT ATTCTGCCAA TGACACTTAC ACCGTGCAGA TCAGCCCCAT GTCGGCCTTT 
GTGGAAAATC ATCTCGAGTG GTTCAGATTT AGCGGTCGCA TCCTTGGGCT TGCCCTGATC 
CACCAGTACC TCCTGGACGC TTTCTTCACG AGGCCGTTCT ACAAGGCCCT GCTGAGACTG 
CCCTGTGATC TGAGCGACCT AGAGTATCTG GATGAGGAAT TCCACCAAAG CCTGCAGTGG 
ATGAAGGACA ATAACATCAC GGATATCTTA GATCTCACTT TCACAGTGAA TGAAGAAGTG 
TTTGGACAGG TAACAGAAAG AGAGTTGAAA TCAGGGGGAG CCAACACCCA GGTGACAGAA 
AAAAATAAAA AGGAGTACAT TGAGAGAATG GTGAAGTGGA GAGTGGAGCG GGGCGTGGTG 
CAGCAGACGG AAGCGTTAGT CCGAGGCTTC TATGAAGTTG TGGATTCAAG GCTCGTCTCC 
GTCTTTGATG CCAGGGAGCT GGAGCTGGTG ATCGCCGGCA CCGCCGAAAT AGACCTCAAT 
GACTGGCGCA ATAACACCGA GTATCGAGGG GGTTATCACG ATGGTCATAT CGTGATCCGG 
TGGTTCTGGG CAGCTGTGGA GAGGTTCAAC AATGAGCAGA GACTGAGGTT GCTTCAGTTC 
GTCACGGGAA CATCGAGCGT ACCCTATGAA GGTTTCGCCG CGCTCCGAGG AAGCAATGGG 
CTGAGGCGCT TCTGTATAGA GAAATGGGGG AAAATAACAT CCCTTCCCAG GGCACACACG 
TGTTTCAACC GCTTGGATCT GCCACCGTAC CCATCGTATT CCATGCTGTA CGAGAAGCTG 
TTGACAGCAG TTGAGGAAAC GAGCACCTTT GGTCTGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007661641.1
CDS	70..4971
Translation	MLCAQPGIIQNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDIRNGPHGTVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIYWDIKEEVGAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSSAPVFKSITNDEATQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQEKRSKITCNTVNPVWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEVSLSADPESAENQESPANHPSEEGLTEPPGQGTEGPEGPELETQDGGGPTSGSGGDSPEATGPAGEKSNEEEAAEGAEAVVEPVDAVDPTEPSPAEDLPSLSAEEIAEGLDLLPGTPPPPPPEDEGWEQGENHAGEADGDPLVGLEEPEGAEAEVEKKVDVSMVLDKDQDEEEEELASPEEDGPLALRPSAKKKSRPCSLPVSELETVIASACGEPETPRTHYIRIHNLLHSMPSAQGGSDGGEAQGPEEEDEEGPGEEESTVKEASEKDVVSEAETVAPDPPHPEDGGESGDPEVSGQRPVPHSASAECLAGVNGGPLLDGEPRPSTGSESDSSPRQDCEGCDTSCYSPSCYSTSCYSTSCYSTSCYSTSCYSPSCYDSNNRFSSHTRFSSVDSAKISESTVFSSQDEEEEENSAFESVPDSVQSPEMDRETADGTGPWPDELVLPNGNVPRTADGLESPIAGPSNRREGDCPLLHNSQPVSQLPSLRPDHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTTAATPDGIRRSGSVQQMEQLNRRYQNIQRTIATERSEDEPPSQSAEPLPAGGGSGGGGGGSDLEADSSQPNLELRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTNSTCLKHMILKIRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRNQHHHETLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHALREKIHYIRTEGTQGLEKLSCDADLVILLSLFEEEIMSYIPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASVRAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_007663451.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007663451.1

ORF Insert Sequence:

ATGCTTTGTG CCCAGCCTGG CATCATTCAG AATCTGTACC AGAACAGGTT CCTGGGTCTG 
GCCGCCATGG CCTCGCCGTC GAGGAACTCC CAGAGCCGGC GCCGCTGTAA AGAGCCCCTG 
CGCTACAGCT ACAACCCCGA CCAGTTCCAC AACATGGACA TCAGGAACGG CCCACACGGG 
ACCGTCACCA TCCCCCGCTC CACCAGCGAC ACCGACCTGG TCACGTCCGA CAGTCGGTCC 
ACCCTTATGG TCAGCAGTTC GTATTACTCC ATTGGACACT CTCAGGACCT GGTGATCTAT 
TGGGATATAA AAGAGGAAGT GGGAGCCGGG GACTGGATTG GCATGTATCT CATCGATGAG 
GTCCTATCTG AAAATTTTCT GGACTACAAA AACCGCGGAG TGAATGGTTC TCACCGGGGC 
CAGATTATCT GGAAGATTGA TGCAAGTTCT TACTTTGTGG AATCTGAAAC TAAGATCTGC 
TTCAAATACT ATCATGGAGT GAGTGGAGCG CTACGAGCCA CTACGCCCAG TGTTACAGTG 
AAAAACTCTT CAGCTCCTGT TTTTAAGAGC ATCACCAATG ACGAGGCCAC CCAGGGTCAA 
GGCAGTCGGA GGCTGATCAG TTTTTCTCTC TCAGATTTCC AGGCCATGGG TCTGAAGAAA 
GGAATGTTCT TTAACCCAGA TCCATATCTG AAGATTTCCA TCCAACCTGG GAAACACAGC 
ATCTTCCCAG CCCTTCCGCA CCATGGTCAG GAGAAGAGGT CGAAGATTAC CTGTAACACC 
GTGAACCCTG TTTGGCAAGC TGAGCAGTTT AGCTTTGTTT CCCTGCCCAC CGACGTCCTA 
GAAATCGAGG TGAAAGACAA GTTTGCCAAG AGTCGTCCAA TCATCAAACG CTTCTTAGGA 
AAGTTGTCCA TGCCTGTTCA GCGACTCCTG GAGAGACACG CTATAGGGGA CAGGGTGGTG 
AGCTACACTC TGGGTCGAAG GCTTCCAACG GATCACGTCA GCGGCCAGCT CCAGTTCCGG 
TTCGAGATCA CCTCCTCCAT CCACCCAGAT GACGAGGAGG TCTCTCTGAG CGCAGATCCC 
GAGTCTGCTG AAAACCAGGA GAGTCCTGCC AACCACCCAT CAGAGGAAGG CCTCACAGAA 
CCTCCAGGCC AGGGGACGGA GGGCCCAGAG GGCCCGGAGC TGGAGACACA GGATGGTGGT 
GGTCCCACGA GTGGCTCTGG GGGAGACAGC CCCGAGGCTA CCGGACCGGC CGGGGAAAAG 
TCGAATGAGG AGGAGGCCGC AGAGGGTGCC GAAGCCGTCG TGGAGCCAGT TGATGCCGTC 
GATCCCACGG AACCCAGCCC GGCCGAAGAT TTGCCTTCCC TCAGTGCTGA AGAGATCGCC 
GAGGGGCTGG ATCTGCTCCC TGGCACTCCC CCGCCACCGC CTCCGGAAGA CGAGGGTTGG 
GAGCAGGGGG AGAACCACGC CGGAGAGGCC GATGGAGATC CCCTTGTCGG CCTGGAGGAG 
CCAGAGGGGG CTGAGGCAGA GGTGGAGAAA AAGGTTGACG TGAGCATGGT TCTAGACAAA 
GACCAGGATG AAGAGGAAGA AGAATTGGCC TCCCCCGAAG AGGATGGGCC TCTGGCGCTG 
AGACCCTCGG CCAAGAAGAA AAGCAGACCG TGCTCCCTGC CCGTGTCCGA GTTGGAGACG 
GTGATCGCGT CGGCCTGCGG CGAACCGGAA ACGCCCCGCA CCCATTACAT CCGCATCCAC 
AACCTGCTGC ACAGCATGCC CTCAGCCCAG GGAGGCAGCG ATGGCGGAGA AGCCCAAGGA 
CCGGAGGAAG AGGACGAGGA GGGGCCGGGG GAGGAAGAGT CAACGGTCAA GGAGGCCTCC 
GAAAAGGACG TGGTCAGCGA GGCCGAGACC GTGGCACCGG ACCCACCTCA CCCAGAAGAT 
GGCGGAGAGA GCGGGGACCC CGAGGTATCT GGCCAGCGGC CGGTGCCCCA CAGTGCCTCG 
GCCGAGTGCT TGGCCGGGGT GAACGGTGGC CCGCTTCTCG ACGGCGAGCC CCGGCCCAGC 
ACGGGGAGCG AGAGCGACTC GAGCCCCCGG CAGGACTGCG AGGGCTGCGA CACATCCTGC 
TACAGCCCGT CCTGCTACAG CACCTCGTGT TACAGCACCT CCTGCTACAG CACCTCGTGC 
TACAGCACCT CCTGCTACAG CCCATCCTGC TACGACAGCA ACAACCGGTT CTCCAGCCAT 
ACGCGCTTCT CCTCGGTGGA CAGTGCCAAG ATCTCGGAGA GCACCGTCTT CTCCTCTCAG 
GACGAGGAGG AGGAGGAGAA CAGCGCGTTC GAGTCGGTGC CCGACTCGGT GCAGAGCCCG 
GAGATGGACC GGGAGACGGC CGACGGCACC GGCCCGTGGC CGGACGAGCT GGTTCTCCCC 
AACGGGAACG TTCCGAGGAC AGCCGATGGC CTGGAGTCCC CGATAGCTGG TCCCAGTAAT 
CGGAGAGAAG GTGATTGTCC TTTACTACAT AATTCTCAGC CAGTCAGCCA GCTTCCTTCC 
TTGAGGCCTG ACCACCACCA TTATCCAACT ATCGATGAGC CTCTTCCGCC AAACTGGGAG 
GCTCGAATAG ACAGTCACGG GAGAGTGTTT TACGTGGACC ACGTGAACCG AACGACGACG 
TGGCAGCGGC CTACCACGGC CGCCACTCCA GACGGGATCC GCAGATCTGG CTCCGTACAG 
CAGATGGAAC AGCTGAACCG CCGGTACCAG AATATCCAGC GGACTATTGC TACCGAGAGA 
TCGGAAGACG AGCCACCCAG TCAGAGTGCG GAGCCTCTCC CTGCCGGAGG AGGGAGTGGA 
GGAGGGGGAG GAGGGAGTGA CTTGGAAGCA GACTCTTCTC AACCAAATCT AGAACTGAGG 
AGAGAGGGGT CTCTTTCTCC TGTGAACTCC CAAAAAATCA CTTTGCTGCT TCAGTCCCCG 
GCCGTCAAGT TCATCACCAA CCCTGAGTTC TTCACCGTGC TGCATGCCAA CTATAGTGCC 
TATAGAGTCT TCACCAACAG CACCTGCTTG AAGCATATGA TTCTGAAGAT CCGCAGGGAT 
GCCCGGAATT TCGAACGCTA CCAGCACAAC CGGGACCTGG TGAACTTCAT CAACATGTTT 
GCTGACACCC GTTTGGAACT GCCTCGCGGC TGGGAAATCA AAACTGACCA GCAGGGCAAG 
TCCTTCTTCG TCGACCACAA CAGTCGAGCC ACCACTTTCA TCGACCCCCG GATCCCTCTT 
CAGAACGGAC GTCTTCCGAA TCACCTGACT CACCGGCAGC ACCTTCAGCG ACTCCGGAGC 
TACAGTGCCG GCGAGGCCTC AGAAGTCTCT CGGAACCGAG GAGCTTCTCT GTTGGCCAGG 
CCGGGTCACA GCCTGGTAGC AGCCATCAGA AACCAACATC ACCATGAGAC GTTACCTCTG 
GCTTATAATG ACAAGATCGT GGCATTTCTC CGCCAGCCAA ACATTTTTGA GATGCTGCAG 
GAGCGCCAGC CCAGCTTGGC CAGAAACCAC GCACTCAGGG AGAAAATCCA TTACATTCGG 
ACAGAAGGTA CTCAAGGGCT TGAAAAATTG TCCTGTGATG CAGATTTAGT AATACTGCTG 
AGCCTCTTTG AAGAAGAGAT CATGTCCTAC ATACCCCTTC AGGCCGCTTT CCACCCGGGA 
TACAGCTTCT CTCCTCGCTG TTCACCGTGC TCTTCCCCTC AAAACTCTCC AGGCCTACAG 
CGAGCCAGTG TCAGGGCCCC CTCACCCTAC AGGAGAGACT TTGAGGCCAA GCTCCGAAAT 
TTCTACCGAA AACTTGAAGC CAAAGGATTT GGTCAAGGTC CGGGTAAAAT CAAACTCATC 
ATAAGACGTG ACCACTTGCT GGAGGGAACC TTCAATCAGG TGATGGCTTA TTCCCGCAAA 
GAGCTCCAGC GGAACAAGCT CTACGTCACA TTTGTTGGAG AGGAGGGCCT AGACTACAGT 
GGGCCCTCCC GGGAGTTCTT TTTCCTTTTG TCCCAGGAGC TCTTCAACCC CTACTACGGC 
CTGTTTGAGT ATTCTGCCAA TGACACTTAC ACCGTGCAGA TCAGCCCCAT GTCGGCCTTT 
GTGGAAAATC ATCTCGAGTG GTTCAGATTT AGCGGTCGCA TCCTTGGGCT TGCCCTGATC 
CACCAGTACC TCCTGGACGC TTTCTTCACG AGGCCGTTCT ACAAGGCCCT GCTGAGACTG 
CCCTGTGATC TGAGCGACCT AGAGTATCTG GATGAGGAAT TCCACCAAAG CCTGCAGTGG 
ATGAAGGACA ATAACATCAC GGATATCTTA GATCTCACTT TCACAGTGAA TGAAGAAGTG 
TTTGGACAGG TAACAGAAAG AGAGTTGAAA TCAGGGGGAG CCAACACCCA GGTGACAGAA 
AAAAATAAAA AGGAGTACAT TGAGAGAATG GTGAAGTGGA GAGTGGAGCG GGGCGTGGTG 
CAGCAGACGG AAGCGTTAGT CCGAGGCTTC TATGAAGTTG TGGATTCAAG GCTCGTCTCC 
GTCTTTGATG CCAGGGAGCT GGAGCTGGTG ATCGCCGGCA CCGCCGAAAT AGACCTCAAT 
GACTGGCGCA ATAACACCGA GTATCGAGGG GGTTATCACG ATGGTCATAT CGTGATCCGG 
TGGTTCTGGG CAGCTGTGGA GAGGTTCAAC AATGAGCAGA GACTGAGGTT GCTTCAGTTC 
GTCACGGGAA CATCGAGCGT ACCCTATGAA GGTTTCGCCG CGCTCCGAGG AAGCAATGGG 
CTGAGGCGCT TCTGTATAGA GAAATGGGGG AAAATAACAT CCCTTCCCAG GGCACACACG 
TGTTTCAACC GCTTGGATCT GCCACCGTAC CCATCGTATT CCATGCTGTA CGAGAAGCTG 
TTGACAGCAG TTGAGGAAAC GAGCACCTTT GGTCTGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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EMAIL

gene@genscript.com

PHONE

1-877-436-7274

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FAX

1-732-210-0262

HECW1 cDNA ORF clone, Ornithorhynchus anatinus(platypus)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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PHONE

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