TTC3 cDNA ORF clone, Ornithorhynchus anatinus(platypus)

Gene
Clones
gRNAs

Summary

Gene Symbol	TTC3
Entrez Gene ID	100078590
Full Name	tetratricopeptide repeat domain 3
Gene Type	protein-coding
Organism	Ornithorhynchus anatinus(platypus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007667267.1	XP_007665457.1	E3 ubiquitin-protein ligase TTC3
XM_029046540.1	XP_028902373.1	E3 ubiquitin-protein ligase TTC3 isoform X1
XM_029046539.1	XP_028902372.1	E3 ubiquitin-protein ligase TTC3 isoform X1
XM_029046541.1	XP_028902374.1	E3 ubiquitin-protein ligase TTC3 isoform X2

Pathways from BioSystems
Gene Ontology

The following TTC3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TTC3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOc120044	XM_029046539.1 Latest version!	Ornithorhynchus anatinus tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOc120044	XM_029046540.1 Latest version!	Ornithorhynchus anatinus tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOc120045	XM_029046541.1 Latest version!	Ornithorhynchus anatinus tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOc07464	XM_007667267.1 Latest version!	Ornithorhynchus anatinus tetratricopeptide repeat domain 3 (TTC3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TTC3 ( XM_029046540.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028902373.1 Ornithorhynchus anatinus tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOc120044
Clone ID Related Accession (Same CDS sequence)	XM_029046540.1 , XM_029046539.1
Accession Version	XM_029046540.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	E3 ubiquitin-protein ligase TTC3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041745.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACT GGTGGACCTC TAATAAGGAA ATTATTAGCT CCGGTTATGT TCGTTTTAAT 
AAATATTGCT GTGAACAGGG TCGTGCAAAT TCTAAAAGAT GTTCTTCAAG CAAGCCAAAA 
TCAGCTCCTG ACATCTGCAA AACCTGGTGC AGCAAACCAC TTCAAGTCCT GCAAGATTAC 
TGTGATATCA TTAAAATATA CATCTTTTGG CCACTTCTCT TTCAACTTGA GCATATGAAT 
TATATAGATT TCAGCAAATC TGTCCATTCT GAGTTAAATT TGAAGAAATT GCAACAGATT 
GAACTGCTGG AAGAAATTGT GGATCTGGGT AAGAAAGTTG CGAATGATCA GTTTTTTATT 
GGTGGCATCC TGAAAATTGG CTCCAAAATA GAAGATAGGA GCCCTGTAAT GGATGAAACC 
TGTAATTGGG TAAAATACAC AGGCGAATTT AAAGTTCTAC ATAAATTAGA AAAATTTGGA 
GATTACTGTT GGCCAATGTT AGAAATTTTC TTTACTGAAT ACAAATATTA TATAACCAAA 
GTTGCCCTGG AAGACTGCAA TTTAGTTGAA GAGTTTGGAT CTCAGAACTG CGATTCCTGT 
ACAAGGAAAG GAGAAATGAT GAAAGAGAGA GGAAATGAAG AATTTTCCCA AAAGAAGTAT 
GATTTGGCAA TCCTTTTCTA CACCAGAGCG ATTAACTTTT GCCCTGAAAA TCACCTTCTT 
TACAGTAACA GAGCTCTTTG TTTTCTTCGA ATTGGACAAT ACAAGAGAGC CCTTGCTGAT 
GGAAAAAGAG CTACTATTCT AAAGCATAAT TGGCCAAAGG GTCATTACCG TTTCTGTGAT 
GCACTCTCAA TGATGGGGGA ACATGAACAG GCCCTAGAAG CAAATGAAAA GGCTCAAAAA 
CTCTGCAGGA ATAATCCTGA AGGAATGAAG GATCTTATTC AGCAGAACAT AAAGTTAAGA 
AAAGAAATGG AAGAAATAAA AGGTGCAAAA CAAAGTAAGT TTCGAATGAA GAAATCACTT 
CATGAGAAAA AATTAACTTC AGATTGTGGT TCTAGAGGGC CTGGGTTTTG TCCTTCACAT 
AATCCAAAGG AAACAGACAA TGAAAAAAGA AGGTTCATAT CGGAATCAAA AGATCATAGT 
TGTCATCAGA AGCGGGATAT AAAGGTGGCT CCTGATGTCC TGAATTCAGA AAAACAAGAG 
TCTCCTTCGG AGTTTCCAGC AGGTCATAAT CAGAAAAATA AAGGAAAAGC AAAAACTAGT 
AGCTGTGATT CTGAAAAGAC TAGAGAACAA CTGAGTTCTA AGATAGATTC TAAAAGCAAT 
TATGATAAGC AGAAGAGTAA ATCTTTGAAT GCTACACAAA AGGTAGCTTT GATTGAGTTA 
ACAGAAATGC TGAAATCCCT TTTCCAAGAT GGTTATACAG CCTTAATGGA CCAGCGTTAC 
CGTGGTGCCG AACAGGCCTT TTCGCAGTTG TTAAACATTT TAGATCCCAA GGAATTAAAG 
CAAATGAATC TTGCCATCAC TGACTACCTT GTCATAATAT ATGGATATGC CACTGCTCTT 
CTTGGAATAG GACAGCCTGA GGGATTATCT GAAGCCGAAA ACCAGTTTAA AAGAATCCTT 
GAGCAGTACT CCAGTGAGGG ATATGATTGC TTGGCATATT ATGGAATCGG GAAGGTTTAT 
TTGAGAAAAA ACAAATTTTC AGATGCTATT TATCATTTTG AGAAATCAAA AACCATGATT 
AAATTTATTC CTGGAGTATT AGTGTGGCCC ACCACTTCTG AGATTATTGA AGAGTCTCAG 
TCTGAGAGAT TACAATCAAT GTTAGAGAAT TATATAGAGG AATGTAAGTT CCCTCCAGAA 
CCAGATGCTG TGTGTTCCTA TCAAAATTGC CATGGACATT CCAAAATCCA GATATATTTA 
ACTGATCCAG ATTTTAAGGG CTTTATACGT ATCAACTGCT GCCAATTGTG TAAAATAGAA 
TTTCACATTA GCTGCTGGAA GAAGTTAAAA ACAACTTTCA ATGATAAAAA TGACAAGGAT 
TTTCTTCAAG ACCCATGTCT TACCCCTGAC TGTGGAGGAA TCATTTCCAA GATTATTATC 
TTTGGTTCAA CAGGCCAAAT TAAATGTGAA TTTGAACATA AAGTTGTGAA AGAAAGGGAT 
CCACCGAGAC CTGTAGTGAA ACAGAAGTGT TCCAGCCTAG GGAAACTAAG AATTAAAGAA 
GATAAAAAAT CAAAGAGAAA GCTTCTAAAA AAAGAAGCAA AATCTTTAGC AAACGAAAGA 
ATGGAAGAGA AATCAAGTGA CAATAACTCA TCTAAACACA GTGTCCATAA AGGCTGTGTT 
CAAAGTTGCC AAGTTATTGG TGATAGAGTT CTGCATTGTA TAAAGCAAAA TGCTGAAAAA 
ATTAAATTTG GGGTACAAGA CACATCTAAA CTTCTGAGTG AATTGCTTTC TTGGTGGGTA 
ATAAGCAAGG AGGATTATAC TGAATGTTCT GCTAGCCATC TACCTAATGA AACCATGGAG 
CGCCTTCTCT GTCACTTGTT CCAGAACCAG AACAGAGTAA AGACAAGAAT TTTTTTACAT 
GTATTGAGTG AGATTGAAGT TGTGGTTGAC CCCAAACTAC ATGAGTGGAT TCAGAAACTC 
AACAATTTTG GCTTGGATGC CACAGAAGCT TTTTTTTCCC GTTATGGAGA TTCTATTAGA 
GAGCTCAAAT GCAACATCAT CACTGATCTT TGGAATGAGA AATATGGTCA TAAACTAGAC 
CTTGTGCTCC CCAATTCTGT AGATGGAGAA ATTCTTGAAT GCTTTCATTT GTCATCCTTG 
AAGGAAGCTC GCTGCCTAAT ATGGCTATTA GAAGAGAATA GGGAAAAATT TCCTACTCTA 
CATCATATTT TAGATGAATT TTTTGATATA ATGGATGGGC CCTGTACTAT ATTAAGGAAA 
CAAGAAAATG AAGATGCCTC AACTGTTATA AAGGTAAAAA ACAAAAACCG GAAAAAGAAG 
CAAAAAGGAT CAAAGCCTGT TTTCCCGCTG TCTGGAGTTG GAGGAATAAT ACAGGATGAT 
GACATTATTT TTACAGATGA AAATCCTCTA TCTTTTTTGA ATTCTTGGGA TCCGTTTGTT 
GTTCCTGCTA ACCTTCAAAA TGAGATAGAA GAATTTGAAG CACTCTATGA AAAAAAGATT 
TCTAATAGTC ATCATTATCA GAGACTATTG GATAATAATC CAGATCCAAA ATGTGAAACA 
CTTTATGACT ATTTCTCTCA AATACTGGAG GAACATGGTC CCCTGGAAAT TACAGATGAG 
TTATTGATTG GCGAATATGA ACACTTCCCT GAAGAAACTC GGAAAGTTGT GGAAGAAGCA 
GGGGGATTGA AATCTTTTCT CCTGAGATCT CTTCGTTTTG TTATGTTTAA CGACTTCATT 
GGACTGATGA AAGATGCAGT TGTCCTGAAG GAAAAGGAAG ATTACATGTT CAGTCCATAT 
TTTGACGAAC ACTCCTCACC AGTCAAACCA TTATTGAATC CAGCTGCAAA GGAATTCAAG 
CCATTCTCCC ATAACTGTAG TTCTAATGTG CCAATGTCTG AAACTATTAA AGAAAACAAT 
CAGACTGAAA AATATGTGGA CCCAGGACAT CCTTCAGCTT TTCCTTTTAA TTATAGTGCT 
GATGTTCGTT ATCACGGATT TCACATCAAT GAAGAATCGA TGTTTTGTTT AAATCTATTA 
CCATATTCAC CTGCCCATTT AAATTTCTAC ACAGCTCCTG ATTATGAACT GGAAAAAATA 
TTACCAGTAG AGGCAGATAG GACAACTGTT GCTGACACTG CTAGACCAAA TAATGATGAT 
GAATATACTG ATCACAATGC TCCTCATTTT GATTCAGAAA AAGTAAAAGA GAGAGTCTTA 
GAAAGAGAGA ACATTTCTGG AAGGATGGTT CTTAGTGAAA CAGAGATATC AGCGAAATTA 
GACAACTGCA TTTATAGAGA TGATAATACT CAACTCCTGG GTAACAGTAA TGCTGGTCAT 
TATATTTCTA CTAATAAAAT GAAAATAGCG ACTAAAGATA TGACATTGGT GAGAAAAAAT 
CCAGCTACAC GAATGATTGC TGTTCAGGTA AATCAGGACT TAACACATCA GGAGGTTAAT 
ACCATGCCTT TTAATCCATT TGAAACCCAA CATGGGGATA TTCTGCGAAT GGAAAAAGAA 
CACCAGGTAT TACAAGAGCA ACTGAGAGAA GCAAATGAAA AATATACACA GTTAAAACAC 
AGGAGTTCAG AAGAGATCAA AGATTTGGAA GAACAATTGA AAGAACTTTC TGAAAAGAAT 
AAGATCTCAA AGACTGAATT GGAATGGATG CACCAGGACC TGGAAAAAGA ACTGAAAAAA 
TGGCAACAGG AAAAAAAAGA AAATCAGGAA AAATTGAAAG GCCTAAAGAG TAAAATAAAG 
AAGTTTATAG AGAGCAATGA GAACTGCTTA AGGACGGTCA AGGAAAAAGA TCAGCAGTAT 
GAATTGCATT TGAATAAATT CCTGGAGATC AGTAATAAAT TTGAAAATGA GAAAGTGAAA 
ATGGAGGAAC ATATAAAGAA GGGAAAAGAT AATTACCAAG AGAGTAATAA AAGATCTGTG 
GCAGCAGAGG TATCGGTCCT GAAAAACTGG AAGGAAAAGG AACTGAGCAA GTTGTTCAGC 
AGAGTATCAG AAGTAGAAAA ATATCTTAAG CAACTAAAGT TAATGAACAG TGATTCATCT 
GAAGTGAAGC CTGAGATAGC TTCTTGGGAG TTGTTTCTTT CAAAAATTAA AAGAATAATT 
GAGGAAACAA AGGCTCAATT TGAAGATAGA ATTCAGAAGA TTCAAAGTGG CTCCCCACTA 
GTCGGACTTT CTAAAGTGCA AATTCCCGAT CTTTCAACTC CTTCCAGTAG TTTAAGAACT 
CTAGTCAACC TTCCTATTAG CGATCCAGCC ATTGTAATGT ATTCTCCACC AATTCATCCT 
AAATTACTTC CTAAATATCC ACACCCTGAA GGTCAAAGTT CAGCGAATCT TCCACGTCCT 
GTACAAACTG GAAACCAAAT GACGACACGT AAAGTCTCAA GTCCGTATCC TGCCAGTGAT 
AGCTCCAAGG GGAAACTTGT GCAAAGAAAT TACCTTGATC CAGCTCTTCC CACTCTGCCT 
TCAGTGACTA CTAGGAATCC TGATGTCCAA GTGAATCACA GCCTAATAGC TGACCGTCTG 
TCACCTAGTA TTCAAAAACA GCCTCCACAG AAGCCATTTG AAGCCATTTT TAGAAATCTG 
GCAATGATTT TCCCCAACTA CAGCAGTAGT GAGTTTCTTT CTTTTATAAA AGAAGTTCAG 
GCCAAAAACA GGGGCACGCT TTCAGGATTG ACCTCGGATG AAATCACCAG GAGAGTGACA 
GATCTCATCC TGGATGAGCC GAGTAAGAAC AAGGCTGGAA AAGATAGGGC AGCGGTATTT 
GTGGCAAATG ACCCTGGCCG AAGATCTTCG GAAGGAAGCG TGTTGGTCTC TGGCACCTCC 
AAGACCAGAC CTGTTTCTAG CAAAAGCCCC AGCCACCCAC CTCGAGGCCA GCCCTGGGTG 
ATAGTTGGGG GGCCAACCAA GACCAAGTGG CAGAAATCGA AAGAGCCCAC TACATCCGAT 
GAAGATCCCT GTATCATCTG TCATGACGAA CTAAATCAGG GCGTCCTTTG CAAACTGGAA 
TGTGGGCATC AGTTTCACAA ACAGTGTATT GACCCCTGGC TAAAAGAACA AAATACATGT 
CCAACCTGCC GGAAACATAT CCTGCTACCA GAGGACTTTC CAGCTCTTTC TTCAGGAAGG 
AAGCCTACCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028902373.1
CDS	264..6095
Translation	MEDWWTSNKEIISSGYVRFNKYCCEQGRANSKRCSSSKPKSAPDICKTWCSKPLQVLQDYCDIIKIYIFWPLLFQLEHMNYIDFSKSVHSELNLKKLQQIELLEEIVDLGKKVANDQFFIGGILKIGSKIEDRSPVMDETCNWVKYTGEFKVLHKLEKFGDYCWPMLEIFFTEYKYYITKVALEDCNLVEEFGSQNCDSCTRKGEMMKERGNEEFSQKKYDLAILFYTRAINFCPENHLLYSNRALCFLRIGQYKRALADGKRATILKHNWPKGHYRFCDALSMMGEHEQALEANEKAQKLCRNNPEGMKDLIQQNIKLRKEMEEIKGAKQSKFRMKKSLHEKKLTSDCGSRGPGFCPSHNPKETDNEKRRFISESKDHSCHQKRDIKVAPDVLNSEKQESPSEFPAGHNQKNKGKAKTSSCDSEKTREQLSSKIDSKSNYDKQKSKSLNATQKVALIELTEMLKSLFQDGYTALMDQRYRGAEQAFSQLLNILDPKELKQMNLAITDYLVIIYGYATALLGIGQPEGLSEAENQFKRILEQYSSEGYDCLAYYGIGKVYLRKNKFSDAIYHFEKSKTMIKFIPGVLVWPTTSEIIEESQSERLQSMLENYIEECKFPPEPDAVCSYQNCHGHSKIQIYLTDPDFKGFIRINCCQLCKIEFHISCWKKLKTTFNDKNDKDFLQDPCLTPDCGGIISKIIIFGSTGQIKCEFEHKVVKERDPPRPVVKQKCSSLGKLRIKEDKKSKRKLLKKEAKSLANERMEEKSSDNNSSKHSVHKGCVQSCQVIGDRVLHCIKQNAEKIKFGVQDTSKLLSELLSWWVISKEDYTECSASHLPNETMERLLCHLFQNQNRVKTRIFLHVLSEIEVVVDPKLHEWIQKLNNFGLDATEAFFSRYGDSIRELKCNIITDLWNEKYGHKLDLVLPNSVDGEILECFHLSSLKEARCLIWLLEENREKFPTLHHILDEFFDIMDGPCTILRKQENEDASTVIKVKNKNRKKKQKGSKPVFPLSGVGGIIQDDDIIFTDENPLSFLNSWDPFVVPANLQNEIEEFEALYEKKISNSHHYQRLLDNNPDPKCETLYDYFSQILEEHGPLEITDELLIGEYEHFPEETRKVVEEAGGLKSFLLRSLRFVMFNDFIGLMKDAVVLKEKEDYMFSPYFDEHSSPVKPLLNPAAKEFKPFSHNCSSNVPMSETIKENNQTEKYVDPGHPSAFPFNYSADVRYHGFHINEESMFCLNLLPYSPAHLNFYTAPDYELEKILPVEADRTTVADTARPNNDDEYTDHNAPHFDSEKVKERVLERENISGRMVLSETEISAKLDNCIYRDDNTQLLGNSNAGHYISTNKMKIATKDMTLVRKNPATRMIAVQVNQDLTHQEVNTMPFNPFETQHGDILRMEKEHQVLQEQLREANEKYTQLKHRSSEEIKDLEEQLKELSEKNKISKTELEWMHQDLEKELKKWQQEKKENQEKLKGLKSKIKKFIESNENCLRTVKEKDQQYELHLNKFLEISNKFENEKVKMEEHIKKGKDNYQESNKRSVAAEVSVLKNWKEKELSKLFSRVSEVEKYLKQLKLMNSDSSEVKPEIASWELFLSKIKRIIEETKAQFEDRIQKIQSGSPLVGLSKVQIPDLSTPSSSLRTLVNLPISDPAIVMYSPPIHPKLLPKYPHPEGQSSANLPRPVQTGNQMTTRKVSSPYPASDSSKGKLVQRNYLDPALPTLPSVTTRNPDVQVNHSLIADRLSPSIQKQPPQKPFEAIFRNLAMIFPNYSSSEFLSFIKEVQAKNRGTLSGLTSDEITRRVTDLILDEPSKNKAGKDRAAVFVANDPGRRSSEGSVLVSGTSKTRPVSSKSPSHPPRGQPWVIVGGPTKTKWQKSKEPTTSDEDPCIICHDELNQGVLCKLECGHQFHKQCIDPWLKEQNTCPTCRKHILLPEDFPALSSGRKPT

Target ORF information:

RefSeq Version	XM_029046540.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029046540.1

ORF Insert Sequence:

ATGGAAGACT GGTGGACCTC TAATAAGGAA ATTATTAGCT CCGGTTATGT TCGTTTTAAT 
AAATATTGCT GTGAACAGGG TCGTGCAAAT TCTAAAAGAT GTTCTTCAAG CAAGCCAAAA 
TCAGCTCCTG ACATCTGCAA AACCTGGTGC AGCAAACCAC TTCAAGTCCT GCAAGATTAC 
TGTGATATCA TTAAAATATA CATCTTTTGG CCACTTCTCT TTCAACTTGA GCATATGAAT 
TATATAGATT TCAGCAAATC TGTCCATTCT GAGTTAAATT TGAAGAAATT GCAACAGATT 
GAACTGCTGG AAGAAATTGT GGATCTGGGT AAGAAAGTTG CGAATGATCA GTTTTTTATT 
GGTGGCATCC TGAAAATTGG CTCCAAAATA GAAGATAGGA GCCCTGTAAT GGATGAAACC 
TGTAATTGGG TAAAATACAC AGGCGAATTT AAAGTTCTAC ATAAATTAGA AAAATTTGGA 
GATTACTGTT GGCCAATGTT AGAAATTTTC TTTACTGAAT ACAAATATTA TATAACCAAA 
GTTGCCCTGG AAGACTGCAA TTTAGTTGAA GAGTTTGGAT CTCAGAACTG CGATTCCTGT 
ACAAGGAAAG GAGAAATGAT GAAAGAGAGA GGAAATGAAG AATTTTCCCA AAAGAAGTAT 
GATTTGGCAA TCCTTTTCTA CACCAGAGCG ATTAACTTTT GCCCTGAAAA TCACCTTCTT 
TACAGTAACA GAGCTCTTTG TTTTCTTCGA ATTGGACAAT ACAAGAGAGC CCTTGCTGAT 
GGAAAAAGAG CTACTATTCT AAAGCATAAT TGGCCAAAGG GTCATTACCG TTTCTGTGAT 
GCACTCTCAA TGATGGGGGA ACATGAACAG GCCCTAGAAG CAAATGAAAA GGCTCAAAAA 
CTCTGCAGGA ATAATCCTGA AGGAATGAAG GATCTTATTC AGCAGAACAT AAAGTTAAGA 
AAAGAAATGG AAGAAATAAA AGGTGCAAAA CAAAGTAAGT TTCGAATGAA GAAATCACTT 
CATGAGAAAA AATTAACTTC AGATTGTGGT TCTAGAGGGC CTGGGTTTTG TCCTTCACAT 
AATCCAAAGG AAACAGACAA TGAAAAAAGA AGGTTCATAT CGGAATCAAA AGATCATAGT 
TGTCATCAGA AGCGGGATAT AAAGGTGGCT CCTGATGTCC TGAATTCAGA AAAACAAGAG 
TCTCCTTCGG AGTTTCCAGC AGGTCATAAT CAGAAAAATA AAGGAAAAGC AAAAACTAGT 
AGCTGTGATT CTGAAAAGAC TAGAGAACAA CTGAGTTCTA AGATAGATTC TAAAAGCAAT 
TATGATAAGC AGAAGAGTAA ATCTTTGAAT GCTACACAAA AGGTAGCTTT GATTGAGTTA 
ACAGAAATGC TGAAATCCCT TTTCCAAGAT GGTTATACAG CCTTAATGGA CCAGCGTTAC 
CGTGGTGCCG AACAGGCCTT TTCGCAGTTG TTAAACATTT TAGATCCCAA GGAATTAAAG 
CAAATGAATC TTGCCATCAC TGACTACCTT GTCATAATAT ATGGATATGC CACTGCTCTT 
CTTGGAATAG GACAGCCTGA GGGATTATCT GAAGCCGAAA ACCAGTTTAA AAGAATCCTT 
GAGCAGTACT CCAGTGAGGG ATATGATTGC TTGGCATATT ATGGAATCGG GAAGGTTTAT 
TTGAGAAAAA ACAAATTTTC AGATGCTATT TATCATTTTG AGAAATCAAA AACCATGATT 
AAATTTATTC CTGGAGTATT AGTGTGGCCC ACCACTTCTG AGATTATTGA AGAGTCTCAG 
TCTGAGAGAT TACAATCAAT GTTAGAGAAT TATATAGAGG AATGTAAGTT CCCTCCAGAA 
CCAGATGCTG TGTGTTCCTA TCAAAATTGC CATGGACATT CCAAAATCCA GATATATTTA 
ACTGATCCAG ATTTTAAGGG CTTTATACGT ATCAACTGCT GCCAATTGTG TAAAATAGAA 
TTTCACATTA GCTGCTGGAA GAAGTTAAAA ACAACTTTCA ATGATAAAAA TGACAAGGAT 
TTTCTTCAAG ACCCATGTCT TACCCCTGAC TGTGGAGGAA TCATTTCCAA GATTATTATC 
TTTGGTTCAA CAGGCCAAAT TAAATGTGAA TTTGAACATA AAGTTGTGAA AGAAAGGGAT 
CCACCGAGAC CTGTAGTGAA ACAGAAGTGT TCCAGCCTAG GGAAACTAAG AATTAAAGAA 
GATAAAAAAT CAAAGAGAAA GCTTCTAAAA AAAGAAGCAA AATCTTTAGC AAACGAAAGA 
ATGGAAGAGA AATCAAGTGA CAATAACTCA TCTAAACACA GTGTCCATAA AGGCTGTGTT 
CAAAGTTGCC AAGTTATTGG TGATAGAGTT CTGCATTGTA TAAAGCAAAA TGCTGAAAAA 
ATTAAATTTG GGGTACAAGA CACATCTAAA CTTCTGAGTG AATTGCTTTC TTGGTGGGTA 
ATAAGCAAGG AGGATTATAC TGAATGTTCT GCTAGCCATC TACCTAATGA AACCATGGAG 
CGCCTTCTCT GTCACTTGTT CCAGAACCAG AACAGAGTAA AGACAAGAAT TTTTTTACAT 
GTATTGAGTG AGATTGAAGT TGTGGTTGAC CCCAAACTAC ATGAGTGGAT TCAGAAACTC 
AACAATTTTG GCTTGGATGC CACAGAAGCT TTTTTTTCCC GTTATGGAGA TTCTATTAGA 
GAGCTCAAAT GCAACATCAT CACTGATCTT TGGAATGAGA AATATGGTCA TAAACTAGAC 
CTTGTGCTCC CCAATTCTGT AGATGGAGAA ATTCTTGAAT GCTTTCATTT GTCATCCTTG 
AAGGAAGCTC GCTGCCTAAT ATGGCTATTA GAAGAGAATA GGGAAAAATT TCCTACTCTA 
CATCATATTT TAGATGAATT TTTTGATATA ATGGATGGGC CCTGTACTAT ATTAAGGAAA 
CAAGAAAATG AAGATGCCTC AACTGTTATA AAGGTAAAAA ACAAAAACCG GAAAAAGAAG 
CAAAAAGGAT CAAAGCCTGT TTTCCCGCTG TCTGGAGTTG GAGGAATAAT ACAGGATGAT 
GACATTATTT TTACAGATGA AAATCCTCTA TCTTTTTTGA ATTCTTGGGA TCCGTTTGTT 
GTTCCTGCTA ACCTTCAAAA TGAGATAGAA GAATTTGAAG CACTCTATGA AAAAAAGATT 
TCTAATAGTC ATCATTATCA GAGACTATTG GATAATAATC CAGATCCAAA ATGTGAAACA 
CTTTATGACT ATTTCTCTCA AATACTGGAG GAACATGGTC CCCTGGAAAT TACAGATGAG 
TTATTGATTG GCGAATATGA ACACTTCCCT GAAGAAACTC GGAAAGTTGT GGAAGAAGCA 
GGGGGATTGA AATCTTTTCT CCTGAGATCT CTTCGTTTTG TTATGTTTAA CGACTTCATT 
GGACTGATGA AAGATGCAGT TGTCCTGAAG GAAAAGGAAG ATTACATGTT CAGTCCATAT 
TTTGACGAAC ACTCCTCACC AGTCAAACCA TTATTGAATC CAGCTGCAAA GGAATTCAAG 
CCATTCTCCC ATAACTGTAG TTCTAATGTG CCAATGTCTG AAACTATTAA AGAAAACAAT 
CAGACTGAAA AATATGTGGA CCCAGGACAT CCTTCAGCTT TTCCTTTTAA TTATAGTGCT 
GATGTTCGTT ATCACGGATT TCACATCAAT GAAGAATCGA TGTTTTGTTT AAATCTATTA 
CCATATTCAC CTGCCCATTT AAATTTCTAC ACAGCTCCTG ATTATGAACT GGAAAAAATA 
TTACCAGTAG AGGCAGATAG GACAACTGTT GCTGACACTG CTAGACCAAA TAATGATGAT 
GAATATACTG ATCACAATGC TCCTCATTTT GATTCAGAAA AAGTAAAAGA GAGAGTCTTA 
GAAAGAGAGA ACATTTCTGG AAGGATGGTT CTTAGTGAAA CAGAGATATC AGCGAAATTA 
GACAACTGCA TTTATAGAGA TGATAATACT CAACTCCTGG GTAACAGTAA TGCTGGTCAT 
TATATTTCTA CTAATAAAAT GAAAATAGCG ACTAAAGATA TGACATTGGT GAGAAAAAAT 
CCAGCTACAC GAATGATTGC TGTTCAGGTA AATCAGGACT TAACACATCA GGAGGTTAAT 
ACCATGCCTT TTAATCCATT TGAAACCCAA CATGGGGATA TTCTGCGAAT GGAAAAAGAA 
CACCAGGTAT TACAAGAGCA ACTGAGAGAA GCAAATGAAA AATATACACA GTTAAAACAC 
AGGAGTTCAG AAGAGATCAA AGATTTGGAA GAACAATTGA AAGAACTTTC TGAAAAGAAT 
AAGATCTCAA AGACTGAATT GGAATGGATG CACCAGGACC TGGAAAAAGA ACTGAAAAAA 
TGGCAACAGG AAAAAAAAGA AAATCAGGAA AAATTGAAAG GCCTAAAGAG TAAAATAAAG 
AAGTTTATAG AGAGCAATGA GAACTGCTTA AGGACGGTCA AGGAAAAAGA TCAGCAGTAT 
GAATTGCATT TGAATAAATT CCTGGAGATC AGTAATAAAT TTGAAAATGA GAAAGTGAAA 
ATGGAGGAAC ATATAAAGAA GGGAAAAGAT AATTACCAAG AGAGTAATAA AAGATCTGTG 
GCAGCAGAGG TATCGGTCCT GAAAAACTGG AAGGAAAAGG AACTGAGCAA GTTGTTCAGC 
AGAGTATCAG AAGTAGAAAA ATATCTTAAG CAACTAAAGT TAATGAACAG TGATTCATCT 
GAAGTGAAGC CTGAGATAGC TTCTTGGGAG TTGTTTCTTT CAAAAATTAA AAGAATAATT 
GAGGAAACAA AGGCTCAATT TGAAGATAGA ATTCAGAAGA TTCAAAGTGG CTCCCCACTA 
GTCGGACTTT CTAAAGTGCA AATTCCCGAT CTTTCAACTC CTTCCAGTAG TTTAAGAACT 
CTAGTCAACC TTCCTATTAG CGATCCAGCC ATTGTAATGT ATTCTCCACC AATTCATCCT 
AAATTACTTC CTAAATATCC ACACCCTGAA GGTCAAAGTT CAGCGAATCT TCCACGTCCT 
GTACAAACTG GAAACCAAAT GACGACACGT AAAGTCTCAA GTCCGTATCC TGCCAGTGAT 
AGCTCCAAGG GGAAACTTGT GCAAAGAAAT TACCTTGATC CAGCTCTTCC CACTCTGCCT 
TCAGTGACTA CTAGGAATCC TGATGTCCAA GTGAATCACA GCCTAATAGC TGACCGTCTG 
TCACCTAGTA TTCAAAAACA GCCTCCACAG AAGCCATTTG AAGCCATTTT TAGAAATCTG 
GCAATGATTT TCCCCAACTA CAGCAGTAGT GAGTTTCTTT CTTTTATAAA AGAAGTTCAG 
GCCAAAAACA GGGGCACGCT TTCAGGATTG ACCTCGGATG AAATCACCAG GAGAGTGACA 
GATCTCATCC TGGATGAGCC GAGTAAGAAC AAGGCTGGAA AAGATAGGGC AGCGGTATTT 
GTGGCAAATG ACCCTGGCCG AAGATCTTCG GAAGGAAGCG TGTTGGTCTC TGGCACCTCC 
AAGACCAGAC CTGTTTCTAG CAAAAGCCCC AGCCACCCAC CTCGAGGCCA GCCCTGGGTG 
ATAGTTGGGG GGCCAACCAA GACCAAGTGG CAGAAATCGA AAGAGCCCAC TACATCCGAT 
GAAGATCCCT GTATCATCTG TCATGACGAA CTAAATCAGG GCGTCCTTTG CAAACTGGAA 
TGTGGGCATC AGTTTCACAA ACAGTGTATT GACCCCTGGC TAAAAGAACA AAATACATGT 
CCAACCTGCC GGAAACATAT CCTGCTACCA GAGGACTTTC CAGCTCTTTC TTCAGGAAGG 
AAGCCTACCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_029046539.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028902372.1 Ornithorhynchus anatinus tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.

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CloneID	OOc120044
Clone ID Related Accession (Same CDS sequence)	XM_029046540.1 , XM_029046539.1
Accession Version	XM_029046539.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	E3 ubiquitin-protein ligase TTC3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041745.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACT GGTGGACCTC TAATAAGGAA ATTATTAGCT CCGGTTATGT TCGTTTTAAT 
AAATATTGCT GTGAACAGGG TCGTGCAAAT TCTAAAAGAT GTTCTTCAAG CAAGCCAAAA 
TCAGCTCCTG ACATCTGCAA AACCTGGTGC AGCAAACCAC TTCAAGTCCT GCAAGATTAC 
TGTGATATCA TTAAAATATA CATCTTTTGG CCACTTCTCT TTCAACTTGA GCATATGAAT 
TATATAGATT TCAGCAAATC TGTCCATTCT GAGTTAAATT TGAAGAAATT GCAACAGATT 
GAACTGCTGG AAGAAATTGT GGATCTGGGT AAGAAAGTTG CGAATGATCA GTTTTTTATT 
GGTGGCATCC TGAAAATTGG CTCCAAAATA GAAGATAGGA GCCCTGTAAT GGATGAAACC 
TGTAATTGGG TAAAATACAC AGGCGAATTT AAAGTTCTAC ATAAATTAGA AAAATTTGGA 
GATTACTGTT GGCCAATGTT AGAAATTTTC TTTACTGAAT ACAAATATTA TATAACCAAA 
GTTGCCCTGG AAGACTGCAA TTTAGTTGAA GAGTTTGGAT CTCAGAACTG CGATTCCTGT 
ACAAGGAAAG GAGAAATGAT GAAAGAGAGA GGAAATGAAG AATTTTCCCA AAAGAAGTAT 
GATTTGGCAA TCCTTTTCTA CACCAGAGCG ATTAACTTTT GCCCTGAAAA TCACCTTCTT 
TACAGTAACA GAGCTCTTTG TTTTCTTCGA ATTGGACAAT ACAAGAGAGC CCTTGCTGAT 
GGAAAAAGAG CTACTATTCT AAAGCATAAT TGGCCAAAGG GTCATTACCG TTTCTGTGAT 
GCACTCTCAA TGATGGGGGA ACATGAACAG GCCCTAGAAG CAAATGAAAA GGCTCAAAAA 
CTCTGCAGGA ATAATCCTGA AGGAATGAAG GATCTTATTC AGCAGAACAT AAAGTTAAGA 
AAAGAAATGG AAGAAATAAA AGGTGCAAAA CAAAGTAAGT TTCGAATGAA GAAATCACTT 
CATGAGAAAA AATTAACTTC AGATTGTGGT TCTAGAGGGC CTGGGTTTTG TCCTTCACAT 
AATCCAAAGG AAACAGACAA TGAAAAAAGA AGGTTCATAT CGGAATCAAA AGATCATAGT 
TGTCATCAGA AGCGGGATAT AAAGGTGGCT CCTGATGTCC TGAATTCAGA AAAACAAGAG 
TCTCCTTCGG AGTTTCCAGC AGGTCATAAT CAGAAAAATA AAGGAAAAGC AAAAACTAGT 
AGCTGTGATT CTGAAAAGAC TAGAGAACAA CTGAGTTCTA AGATAGATTC TAAAAGCAAT 
TATGATAAGC AGAAGAGTAA ATCTTTGAAT GCTACACAAA AGGTAGCTTT GATTGAGTTA 
ACAGAAATGC TGAAATCCCT TTTCCAAGAT GGTTATACAG CCTTAATGGA CCAGCGTTAC 
CGTGGTGCCG AACAGGCCTT TTCGCAGTTG TTAAACATTT TAGATCCCAA GGAATTAAAG 
CAAATGAATC TTGCCATCAC TGACTACCTT GTCATAATAT ATGGATATGC CACTGCTCTT 
CTTGGAATAG GACAGCCTGA GGGATTATCT GAAGCCGAAA ACCAGTTTAA AAGAATCCTT 
GAGCAGTACT CCAGTGAGGG ATATGATTGC TTGGCATATT ATGGAATCGG GAAGGTTTAT 
TTGAGAAAAA ACAAATTTTC AGATGCTATT TATCATTTTG AGAAATCAAA AACCATGATT 
AAATTTATTC CTGGAGTATT AGTGTGGCCC ACCACTTCTG AGATTATTGA AGAGTCTCAG 
TCTGAGAGAT TACAATCAAT GTTAGAGAAT TATATAGAGG AATGTAAGTT CCCTCCAGAA 
CCAGATGCTG TGTGTTCCTA TCAAAATTGC CATGGACATT CCAAAATCCA GATATATTTA 
ACTGATCCAG ATTTTAAGGG CTTTATACGT ATCAACTGCT GCCAATTGTG TAAAATAGAA 
TTTCACATTA GCTGCTGGAA GAAGTTAAAA ACAACTTTCA ATGATAAAAA TGACAAGGAT 
TTTCTTCAAG ACCCATGTCT TACCCCTGAC TGTGGAGGAA TCATTTCCAA GATTATTATC 
TTTGGTTCAA CAGGCCAAAT TAAATGTGAA TTTGAACATA AAGTTGTGAA AGAAAGGGAT 
CCACCGAGAC CTGTAGTGAA ACAGAAGTGT TCCAGCCTAG GGAAACTAAG AATTAAAGAA 
GATAAAAAAT CAAAGAGAAA GCTTCTAAAA AAAGAAGCAA AATCTTTAGC AAACGAAAGA 
ATGGAAGAGA AATCAAGTGA CAATAACTCA TCTAAACACA GTGTCCATAA AGGCTGTGTT 
CAAAGTTGCC AAGTTATTGG TGATAGAGTT CTGCATTGTA TAAAGCAAAA TGCTGAAAAA 
ATTAAATTTG GGGTACAAGA CACATCTAAA CTTCTGAGTG AATTGCTTTC TTGGTGGGTA 
ATAAGCAAGG AGGATTATAC TGAATGTTCT GCTAGCCATC TACCTAATGA AACCATGGAG 
CGCCTTCTCT GTCACTTGTT CCAGAACCAG AACAGAGTAA AGACAAGAAT TTTTTTACAT 
GTATTGAGTG AGATTGAAGT TGTGGTTGAC CCCAAACTAC ATGAGTGGAT TCAGAAACTC 
AACAATTTTG GCTTGGATGC CACAGAAGCT TTTTTTTCCC GTTATGGAGA TTCTATTAGA 
GAGCTCAAAT GCAACATCAT CACTGATCTT TGGAATGAGA AATATGGTCA TAAACTAGAC 
CTTGTGCTCC CCAATTCTGT AGATGGAGAA ATTCTTGAAT GCTTTCATTT GTCATCCTTG 
AAGGAAGCTC GCTGCCTAAT ATGGCTATTA GAAGAGAATA GGGAAAAATT TCCTACTCTA 
CATCATATTT TAGATGAATT TTTTGATATA ATGGATGGGC CCTGTACTAT ATTAAGGAAA 
CAAGAAAATG AAGATGCCTC AACTGTTATA AAGGTAAAAA ACAAAAACCG GAAAAAGAAG 
CAAAAAGGAT CAAAGCCTGT TTTCCCGCTG TCTGGAGTTG GAGGAATAAT ACAGGATGAT 
GACATTATTT TTACAGATGA AAATCCTCTA TCTTTTTTGA ATTCTTGGGA TCCGTTTGTT 
GTTCCTGCTA ACCTTCAAAA TGAGATAGAA GAATTTGAAG CACTCTATGA AAAAAAGATT 
TCTAATAGTC ATCATTATCA GAGACTATTG GATAATAATC CAGATCCAAA ATGTGAAACA 
CTTTATGACT ATTTCTCTCA AATACTGGAG GAACATGGTC CCCTGGAAAT TACAGATGAG 
TTATTGATTG GCGAATATGA ACACTTCCCT GAAGAAACTC GGAAAGTTGT GGAAGAAGCA 
GGGGGATTGA AATCTTTTCT CCTGAGATCT CTTCGTTTTG TTATGTTTAA CGACTTCATT 
GGACTGATGA AAGATGCAGT TGTCCTGAAG GAAAAGGAAG ATTACATGTT CAGTCCATAT 
TTTGACGAAC ACTCCTCACC AGTCAAACCA TTATTGAATC CAGCTGCAAA GGAATTCAAG 
CCATTCTCCC ATAACTGTAG TTCTAATGTG CCAATGTCTG AAACTATTAA AGAAAACAAT 
CAGACTGAAA AATATGTGGA CCCAGGACAT CCTTCAGCTT TTCCTTTTAA TTATAGTGCT 
GATGTTCGTT ATCACGGATT TCACATCAAT GAAGAATCGA TGTTTTGTTT AAATCTATTA 
CCATATTCAC CTGCCCATTT AAATTTCTAC ACAGCTCCTG ATTATGAACT GGAAAAAATA 
TTACCAGTAG AGGCAGATAG GACAACTGTT GCTGACACTG CTAGACCAAA TAATGATGAT 
GAATATACTG ATCACAATGC TCCTCATTTT GATTCAGAAA AAGTAAAAGA GAGAGTCTTA 
GAAAGAGAGA ACATTTCTGG AAGGATGGTT CTTAGTGAAA CAGAGATATC AGCGAAATTA 
GACAACTGCA TTTATAGAGA TGATAATACT CAACTCCTGG GTAACAGTAA TGCTGGTCAT 
TATATTTCTA CTAATAAAAT GAAAATAGCG ACTAAAGATA TGACATTGGT GAGAAAAAAT 
CCAGCTACAC GAATGATTGC TGTTCAGGTA AATCAGGACT TAACACATCA GGAGGTTAAT 
ACCATGCCTT TTAATCCATT TGAAACCCAA CATGGGGATA TTCTGCGAAT GGAAAAAGAA 
CACCAGGTAT TACAAGAGCA ACTGAGAGAA GCAAATGAAA AATATACACA GTTAAAACAC 
AGGAGTTCAG AAGAGATCAA AGATTTGGAA GAACAATTGA AAGAACTTTC TGAAAAGAAT 
AAGATCTCAA AGACTGAATT GGAATGGATG CACCAGGACC TGGAAAAAGA ACTGAAAAAA 
TGGCAACAGG AAAAAAAAGA AAATCAGGAA AAATTGAAAG GCCTAAAGAG TAAAATAAAG 
AAGTTTATAG AGAGCAATGA GAACTGCTTA AGGACGGTCA AGGAAAAAGA TCAGCAGTAT 
GAATTGCATT TGAATAAATT CCTGGAGATC AGTAATAAAT TTGAAAATGA GAAAGTGAAA 
ATGGAGGAAC ATATAAAGAA GGGAAAAGAT AATTACCAAG AGAGTAATAA AAGATCTGTG 
GCAGCAGAGG TATCGGTCCT GAAAAACTGG AAGGAAAAGG AACTGAGCAA GTTGTTCAGC 
AGAGTATCAG AAGTAGAAAA ATATCTTAAG CAACTAAAGT TAATGAACAG TGATTCATCT 
GAAGTGAAGC CTGAGATAGC TTCTTGGGAG TTGTTTCTTT CAAAAATTAA AAGAATAATT 
GAGGAAACAA AGGCTCAATT TGAAGATAGA ATTCAGAAGA TTCAAAGTGG CTCCCCACTA 
GTCGGACTTT CTAAAGTGCA AATTCCCGAT CTTTCAACTC CTTCCAGTAG TTTAAGAACT 
CTAGTCAACC TTCCTATTAG CGATCCAGCC ATTGTAATGT ATTCTCCACC AATTCATCCT 
AAATTACTTC CTAAATATCC ACACCCTGAA GGTCAAAGTT CAGCGAATCT TCCACGTCCT 
GTACAAACTG GAAACCAAAT GACGACACGT AAAGTCTCAA GTCCGTATCC TGCCAGTGAT 
AGCTCCAAGG GGAAACTTGT GCAAAGAAAT TACCTTGATC CAGCTCTTCC CACTCTGCCT 
TCAGTGACTA CTAGGAATCC TGATGTCCAA GTGAATCACA GCCTAATAGC TGACCGTCTG 
TCACCTAGTA TTCAAAAACA GCCTCCACAG AAGCCATTTG AAGCCATTTT TAGAAATCTG 
GCAATGATTT TCCCCAACTA CAGCAGTAGT GAGTTTCTTT CTTTTATAAA AGAAGTTCAG 
GCCAAAAACA GGGGCACGCT TTCAGGATTG ACCTCGGATG AAATCACCAG GAGAGTGACA 
GATCTCATCC TGGATGAGCC GAGTAAGAAC AAGGCTGGAA AAGATAGGGC AGCGGTATTT 
GTGGCAAATG ACCCTGGCCG AAGATCTTCG GAAGGAAGCG TGTTGGTCTC TGGCACCTCC 
AAGACCAGAC CTGTTTCTAG CAAAAGCCCC AGCCACCCAC CTCGAGGCCA GCCCTGGGTG 
ATAGTTGGGG GGCCAACCAA GACCAAGTGG CAGAAATCGA AAGAGCCCAC TACATCCGAT 
GAAGATCCCT GTATCATCTG TCATGACGAA CTAAATCAGG GCGTCCTTTG CAAACTGGAA 
TGTGGGCATC AGTTTCACAA ACAGTGTATT GACCCCTGGC TAAAAGAACA AAATACATGT 
CCAACCTGCC GGAAACATAT CCTGCTACCA GAGGACTTTC CAGCTCTTTC TTCAGGAAGG 
AAGCCTACCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028902372.1
CDS	108..5939
Translation	MEDWWTSNKEIISSGYVRFNKYCCEQGRANSKRCSSSKPKSAPDICKTWCSKPLQVLQDYCDIIKIYIFWPLLFQLEHMNYIDFSKSVHSELNLKKLQQIELLEEIVDLGKKVANDQFFIGGILKIGSKIEDRSPVMDETCNWVKYTGEFKVLHKLEKFGDYCWPMLEIFFTEYKYYITKVALEDCNLVEEFGSQNCDSCTRKGEMMKERGNEEFSQKKYDLAILFYTRAINFCPENHLLYSNRALCFLRIGQYKRALADGKRATILKHNWPKGHYRFCDALSMMGEHEQALEANEKAQKLCRNNPEGMKDLIQQNIKLRKEMEEIKGAKQSKFRMKKSLHEKKLTSDCGSRGPGFCPSHNPKETDNEKRRFISESKDHSCHQKRDIKVAPDVLNSEKQESPSEFPAGHNQKNKGKAKTSSCDSEKTREQLSSKIDSKSNYDKQKSKSLNATQKVALIELTEMLKSLFQDGYTALMDQRYRGAEQAFSQLLNILDPKELKQMNLAITDYLVIIYGYATALLGIGQPEGLSEAENQFKRILEQYSSEGYDCLAYYGIGKVYLRKNKFSDAIYHFEKSKTMIKFIPGVLVWPTTSEIIEESQSERLQSMLENYIEECKFPPEPDAVCSYQNCHGHSKIQIYLTDPDFKGFIRINCCQLCKIEFHISCWKKLKTTFNDKNDKDFLQDPCLTPDCGGIISKIIIFGSTGQIKCEFEHKVVKERDPPRPVVKQKCSSLGKLRIKEDKKSKRKLLKKEAKSLANERMEEKSSDNNSSKHSVHKGCVQSCQVIGDRVLHCIKQNAEKIKFGVQDTSKLLSELLSWWVISKEDYTECSASHLPNETMERLLCHLFQNQNRVKTRIFLHVLSEIEVVVDPKLHEWIQKLNNFGLDATEAFFSRYGDSIRELKCNIITDLWNEKYGHKLDLVLPNSVDGEILECFHLSSLKEARCLIWLLEENREKFPTLHHILDEFFDIMDGPCTILRKQENEDASTVIKVKNKNRKKKQKGSKPVFPLSGVGGIIQDDDIIFTDENPLSFLNSWDPFVVPANLQNEIEEFEALYEKKISNSHHYQRLLDNNPDPKCETLYDYFSQILEEHGPLEITDELLIGEYEHFPEETRKVVEEAGGLKSFLLRSLRFVMFNDFIGLMKDAVVLKEKEDYMFSPYFDEHSSPVKPLLNPAAKEFKPFSHNCSSNVPMSETIKENNQTEKYVDPGHPSAFPFNYSADVRYHGFHINEESMFCLNLLPYSPAHLNFYTAPDYELEKILPVEADRTTVADTARPNNDDEYTDHNAPHFDSEKVKERVLERENISGRMVLSETEISAKLDNCIYRDDNTQLLGNSNAGHYISTNKMKIATKDMTLVRKNPATRMIAVQVNQDLTHQEVNTMPFNPFETQHGDILRMEKEHQVLQEQLREANEKYTQLKHRSSEEIKDLEEQLKELSEKNKISKTELEWMHQDLEKELKKWQQEKKENQEKLKGLKSKIKKFIESNENCLRTVKEKDQQYELHLNKFLEISNKFENEKVKMEEHIKKGKDNYQESNKRSVAAEVSVLKNWKEKELSKLFSRVSEVEKYLKQLKLMNSDSSEVKPEIASWELFLSKIKRIIEETKAQFEDRIQKIQSGSPLVGLSKVQIPDLSTPSSSLRTLVNLPISDPAIVMYSPPIHPKLLPKYPHPEGQSSANLPRPVQTGNQMTTRKVSSPYPASDSSKGKLVQRNYLDPALPTLPSVTTRNPDVQVNHSLIADRLSPSIQKQPPQKPFEAIFRNLAMIFPNYSSSEFLSFIKEVQAKNRGTLSGLTSDEITRRVTDLILDEPSKNKAGKDRAAVFVANDPGRRSSEGSVLVSGTSKTRPVSSKSPSHPPRGQPWVIVGGPTKTKWQKSKEPTTSDEDPCIICHDELNQGVLCKLECGHQFHKQCIDPWLKEQNTCPTCRKHILLPEDFPALSSGRKPT

Target ORF information:

RefSeq Version	XM_029046539.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029046539.1

ORF Insert Sequence:

ATGGAAGACT GGTGGACCTC TAATAAGGAA ATTATTAGCT CCGGTTATGT TCGTTTTAAT 
AAATATTGCT GTGAACAGGG TCGTGCAAAT TCTAAAAGAT GTTCTTCAAG CAAGCCAAAA 
TCAGCTCCTG ACATCTGCAA AACCTGGTGC AGCAAACCAC TTCAAGTCCT GCAAGATTAC 
TGTGATATCA TTAAAATATA CATCTTTTGG CCACTTCTCT TTCAACTTGA GCATATGAAT 
TATATAGATT TCAGCAAATC TGTCCATTCT GAGTTAAATT TGAAGAAATT GCAACAGATT 
GAACTGCTGG AAGAAATTGT GGATCTGGGT AAGAAAGTTG CGAATGATCA GTTTTTTATT 
GGTGGCATCC TGAAAATTGG CTCCAAAATA GAAGATAGGA GCCCTGTAAT GGATGAAACC 
TGTAATTGGG TAAAATACAC AGGCGAATTT AAAGTTCTAC ATAAATTAGA AAAATTTGGA 
GATTACTGTT GGCCAATGTT AGAAATTTTC TTTACTGAAT ACAAATATTA TATAACCAAA 
GTTGCCCTGG AAGACTGCAA TTTAGTTGAA GAGTTTGGAT CTCAGAACTG CGATTCCTGT 
ACAAGGAAAG GAGAAATGAT GAAAGAGAGA GGAAATGAAG AATTTTCCCA AAAGAAGTAT 
GATTTGGCAA TCCTTTTCTA CACCAGAGCG ATTAACTTTT GCCCTGAAAA TCACCTTCTT 
TACAGTAACA GAGCTCTTTG TTTTCTTCGA ATTGGACAAT ACAAGAGAGC CCTTGCTGAT 
GGAAAAAGAG CTACTATTCT AAAGCATAAT TGGCCAAAGG GTCATTACCG TTTCTGTGAT 
GCACTCTCAA TGATGGGGGA ACATGAACAG GCCCTAGAAG CAAATGAAAA GGCTCAAAAA 
CTCTGCAGGA ATAATCCTGA AGGAATGAAG GATCTTATTC AGCAGAACAT AAAGTTAAGA 
AAAGAAATGG AAGAAATAAA AGGTGCAAAA CAAAGTAAGT TTCGAATGAA GAAATCACTT 
CATGAGAAAA AATTAACTTC AGATTGTGGT TCTAGAGGGC CTGGGTTTTG TCCTTCACAT 
AATCCAAAGG AAACAGACAA TGAAAAAAGA AGGTTCATAT CGGAATCAAA AGATCATAGT 
TGTCATCAGA AGCGGGATAT AAAGGTGGCT CCTGATGTCC TGAATTCAGA AAAACAAGAG 
TCTCCTTCGG AGTTTCCAGC AGGTCATAAT CAGAAAAATA AAGGAAAAGC AAAAACTAGT 
AGCTGTGATT CTGAAAAGAC TAGAGAACAA CTGAGTTCTA AGATAGATTC TAAAAGCAAT 
TATGATAAGC AGAAGAGTAA ATCTTTGAAT GCTACACAAA AGGTAGCTTT GATTGAGTTA 
ACAGAAATGC TGAAATCCCT TTTCCAAGAT GGTTATACAG CCTTAATGGA CCAGCGTTAC 
CGTGGTGCCG AACAGGCCTT TTCGCAGTTG TTAAACATTT TAGATCCCAA GGAATTAAAG 
CAAATGAATC TTGCCATCAC TGACTACCTT GTCATAATAT ATGGATATGC CACTGCTCTT 
CTTGGAATAG GACAGCCTGA GGGATTATCT GAAGCCGAAA ACCAGTTTAA AAGAATCCTT 
GAGCAGTACT CCAGTGAGGG ATATGATTGC TTGGCATATT ATGGAATCGG GAAGGTTTAT 
TTGAGAAAAA ACAAATTTTC AGATGCTATT TATCATTTTG AGAAATCAAA AACCATGATT 
AAATTTATTC CTGGAGTATT AGTGTGGCCC ACCACTTCTG AGATTATTGA AGAGTCTCAG 
TCTGAGAGAT TACAATCAAT GTTAGAGAAT TATATAGAGG AATGTAAGTT CCCTCCAGAA 
CCAGATGCTG TGTGTTCCTA TCAAAATTGC CATGGACATT CCAAAATCCA GATATATTTA 
ACTGATCCAG ATTTTAAGGG CTTTATACGT ATCAACTGCT GCCAATTGTG TAAAATAGAA 
TTTCACATTA GCTGCTGGAA GAAGTTAAAA ACAACTTTCA ATGATAAAAA TGACAAGGAT 
TTTCTTCAAG ACCCATGTCT TACCCCTGAC TGTGGAGGAA TCATTTCCAA GATTATTATC 
TTTGGTTCAA CAGGCCAAAT TAAATGTGAA TTTGAACATA AAGTTGTGAA AGAAAGGGAT 
CCACCGAGAC CTGTAGTGAA ACAGAAGTGT TCCAGCCTAG GGAAACTAAG AATTAAAGAA 
GATAAAAAAT CAAAGAGAAA GCTTCTAAAA AAAGAAGCAA AATCTTTAGC AAACGAAAGA 
ATGGAAGAGA AATCAAGTGA CAATAACTCA TCTAAACACA GTGTCCATAA AGGCTGTGTT 
CAAAGTTGCC AAGTTATTGG TGATAGAGTT CTGCATTGTA TAAAGCAAAA TGCTGAAAAA 
ATTAAATTTG GGGTACAAGA CACATCTAAA CTTCTGAGTG AATTGCTTTC TTGGTGGGTA 
ATAAGCAAGG AGGATTATAC TGAATGTTCT GCTAGCCATC TACCTAATGA AACCATGGAG 
CGCCTTCTCT GTCACTTGTT CCAGAACCAG AACAGAGTAA AGACAAGAAT TTTTTTACAT 
GTATTGAGTG AGATTGAAGT TGTGGTTGAC CCCAAACTAC ATGAGTGGAT TCAGAAACTC 
AACAATTTTG GCTTGGATGC CACAGAAGCT TTTTTTTCCC GTTATGGAGA TTCTATTAGA 
GAGCTCAAAT GCAACATCAT CACTGATCTT TGGAATGAGA AATATGGTCA TAAACTAGAC 
CTTGTGCTCC CCAATTCTGT AGATGGAGAA ATTCTTGAAT GCTTTCATTT GTCATCCTTG 
AAGGAAGCTC GCTGCCTAAT ATGGCTATTA GAAGAGAATA GGGAAAAATT TCCTACTCTA 
CATCATATTT TAGATGAATT TTTTGATATA ATGGATGGGC CCTGTACTAT ATTAAGGAAA 
CAAGAAAATG AAGATGCCTC AACTGTTATA AAGGTAAAAA ACAAAAACCG GAAAAAGAAG 
CAAAAAGGAT CAAAGCCTGT TTTCCCGCTG TCTGGAGTTG GAGGAATAAT ACAGGATGAT 
GACATTATTT TTACAGATGA AAATCCTCTA TCTTTTTTGA ATTCTTGGGA TCCGTTTGTT 
GTTCCTGCTA ACCTTCAAAA TGAGATAGAA GAATTTGAAG CACTCTATGA AAAAAAGATT 
TCTAATAGTC ATCATTATCA GAGACTATTG GATAATAATC CAGATCCAAA ATGTGAAACA 
CTTTATGACT ATTTCTCTCA AATACTGGAG GAACATGGTC CCCTGGAAAT TACAGATGAG 
TTATTGATTG GCGAATATGA ACACTTCCCT GAAGAAACTC GGAAAGTTGT GGAAGAAGCA 
GGGGGATTGA AATCTTTTCT CCTGAGATCT CTTCGTTTTG TTATGTTTAA CGACTTCATT 
GGACTGATGA AAGATGCAGT TGTCCTGAAG GAAAAGGAAG ATTACATGTT CAGTCCATAT 
TTTGACGAAC ACTCCTCACC AGTCAAACCA TTATTGAATC CAGCTGCAAA GGAATTCAAG 
CCATTCTCCC ATAACTGTAG TTCTAATGTG CCAATGTCTG AAACTATTAA AGAAAACAAT 
CAGACTGAAA AATATGTGGA CCCAGGACAT CCTTCAGCTT TTCCTTTTAA TTATAGTGCT 
GATGTTCGTT ATCACGGATT TCACATCAAT GAAGAATCGA TGTTTTGTTT AAATCTATTA 
CCATATTCAC CTGCCCATTT AAATTTCTAC ACAGCTCCTG ATTATGAACT GGAAAAAATA 
TTACCAGTAG AGGCAGATAG GACAACTGTT GCTGACACTG CTAGACCAAA TAATGATGAT 
GAATATACTG ATCACAATGC TCCTCATTTT GATTCAGAAA AAGTAAAAGA GAGAGTCTTA 
GAAAGAGAGA ACATTTCTGG AAGGATGGTT CTTAGTGAAA CAGAGATATC AGCGAAATTA 
GACAACTGCA TTTATAGAGA TGATAATACT CAACTCCTGG GTAACAGTAA TGCTGGTCAT 
TATATTTCTA CTAATAAAAT GAAAATAGCG ACTAAAGATA TGACATTGGT GAGAAAAAAT 
CCAGCTACAC GAATGATTGC TGTTCAGGTA AATCAGGACT TAACACATCA GGAGGTTAAT 
ACCATGCCTT TTAATCCATT TGAAACCCAA CATGGGGATA TTCTGCGAAT GGAAAAAGAA 
CACCAGGTAT TACAAGAGCA ACTGAGAGAA GCAAATGAAA AATATACACA GTTAAAACAC 
AGGAGTTCAG AAGAGATCAA AGATTTGGAA GAACAATTGA AAGAACTTTC TGAAAAGAAT 
AAGATCTCAA AGACTGAATT GGAATGGATG CACCAGGACC TGGAAAAAGA ACTGAAAAAA 
TGGCAACAGG AAAAAAAAGA AAATCAGGAA AAATTGAAAG GCCTAAAGAG TAAAATAAAG 
AAGTTTATAG AGAGCAATGA GAACTGCTTA AGGACGGTCA AGGAAAAAGA TCAGCAGTAT 
GAATTGCATT TGAATAAATT CCTGGAGATC AGTAATAAAT TTGAAAATGA GAAAGTGAAA 
ATGGAGGAAC ATATAAAGAA GGGAAAAGAT AATTACCAAG AGAGTAATAA AAGATCTGTG 
GCAGCAGAGG TATCGGTCCT GAAAAACTGG AAGGAAAAGG AACTGAGCAA GTTGTTCAGC 
AGAGTATCAG AAGTAGAAAA ATATCTTAAG CAACTAAAGT TAATGAACAG TGATTCATCT 
GAAGTGAAGC CTGAGATAGC TTCTTGGGAG TTGTTTCTTT CAAAAATTAA AAGAATAATT 
GAGGAAACAA AGGCTCAATT TGAAGATAGA ATTCAGAAGA TTCAAAGTGG CTCCCCACTA 
GTCGGACTTT CTAAAGTGCA AATTCCCGAT CTTTCAACTC CTTCCAGTAG TTTAAGAACT 
CTAGTCAACC TTCCTATTAG CGATCCAGCC ATTGTAATGT ATTCTCCACC AATTCATCCT 
AAATTACTTC CTAAATATCC ACACCCTGAA GGTCAAAGTT CAGCGAATCT TCCACGTCCT 
GTACAAACTG GAAACCAAAT GACGACACGT AAAGTCTCAA GTCCGTATCC TGCCAGTGAT 
AGCTCCAAGG GGAAACTTGT GCAAAGAAAT TACCTTGATC CAGCTCTTCC CACTCTGCCT 
TCAGTGACTA CTAGGAATCC TGATGTCCAA GTGAATCACA GCCTAATAGC TGACCGTCTG 
TCACCTAGTA TTCAAAAACA GCCTCCACAG AAGCCATTTG AAGCCATTTT TAGAAATCTG 
GCAATGATTT TCCCCAACTA CAGCAGTAGT GAGTTTCTTT CTTTTATAAA AGAAGTTCAG 
GCCAAAAACA GGGGCACGCT TTCAGGATTG ACCTCGGATG AAATCACCAG GAGAGTGACA 
GATCTCATCC TGGATGAGCC GAGTAAGAAC AAGGCTGGAA AAGATAGGGC AGCGGTATTT 
GTGGCAAATG ACCCTGGCCG AAGATCTTCG GAAGGAAGCG TGTTGGTCTC TGGCACCTCC 
AAGACCAGAC CTGTTTCTAG CAAAAGCCCC AGCCACCCAC CTCGAGGCCA GCCCTGGGTG 
ATAGTTGGGG GGCCAACCAA GACCAAGTGG CAGAAATCGA AAGAGCCCAC TACATCCGAT 
GAAGATCCCT GTATCATCTG TCATGACGAA CTAAATCAGG GCGTCCTTTG CAAACTGGAA 
TGTGGGCATC AGTTTCACAA ACAGTGTATT GACCCCTGGC TAAAAGAACA AAATACATGT 
CCAACCTGCC GGAAACATAT CCTGCTACCA GAGGACTTTC CAGCTCTTTC TTCAGGAAGG 
AAGCCTACCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_029046541.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028902374.1 Ornithorhynchus anatinus tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.

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CloneID	OOc120045
Clone ID Related Accession (Same CDS sequence)	XM_029046541.1
Accession Version	XM_029046541.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	E3 ubiquitin-protein ligase TTC3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041745.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACT GGTGGACCTC TAATAAGGAA ATTATTAGCT CCGGTTATGT TCGTTTTAAT 
AAATATTGCT GTGAACAGGG TCGTGCAAAT TCTAAAAGAT GTTCTTCAAG CAAGCCAAAA 
TCAGCTCCTG ACATCTGCAA AACCTGGTGC AGCAAACCAC TTCAAGTCCT GCAAGATTAC 
TGTGATATCA TTAAAATATA CATCTTTTGG CCACTTCTCT TTCAACTTGA GCATATGAAT 
TATATAGATT TCAGCAAATC TGTCCATTCT GAGTTAAATT TGAAGAAATT GCAACAGATT 
GAACTGCTGG AAGAAATTGT GGATCTGGGT AAGAAAGTTG CGAATGATCA GTTTTTTATT 
GGTGGCATCC TGAAAATTGG CTCCAAAATA GAAGATAGGA GCCCTGTAAT GGATGAAACC 
TGTAATTGGG TAAAATACAC AGGCGAATTT AAAGTTCTAC ATAAATTAGA AAAATTTGGA 
GATTACTGTT GGCCAATGTT AGAAATTTTC TTTACTGAAT ACAAATATTA TATAACCAAA 
GTTGCCCTGG AAGACTGCAA TTTAGTTGAA GAGTTTGGAT CTCAGAACTG CGATTCCTGT 
ACAAGGAAAG GAGAAATGAT GAAAGAGAGA GGAAATGAAG AATTTTCCCA AAAGAAGTAT 
GATTTGGCAA TCCTTTTCTA CACCAGAGCG ATTAACTTTT GCCCTGAAAA TCACCTTCTT 
TACAGTAACA GAGCTCTTTG TTTTCTTCGA ATTGGACAAT ACAAGAGAGC CCTTGCTGAT 
GGAAAAAGAG CTACTATTCT AAAGCATAAT TGGCCAAAGG GTCATTACCG TTTCTGTGAT 
GCACTCTCAA TGATGGGGGA ACATGAACAG GCCCTAGAAG CAAATGAAAA GGCTCAAAAA 
CTCTGCAGGA ATAATCCTGA AGGAATGAAG GATCTTATTC AGCAGAACAT AAAGTTAAGA 
AAAGAAATGG AAGAAATAAA AGGTGCAAAA CAAAGTAAGT TTCGAATGAA GAAATCACTT 
CATGAGAAAA AAGGGCCTGG GTTTTGTCCT TCACATAATC CAAAGGAAAC AGACAATGAA 
AAAAGAAGGT TCATATCGGA ATCAAAAGAT CATAGTTGTC ATCAGAAGCG GGATATAAAG 
GTGGCTCCTG ATGTCCTGAA TTCAGAAAAA CAAGAGTCTC CTTCGGAGTT TCCAGCAGGT 
CATAATCAGA AAAATAAAGG AAAAGCAAAA ACTAGTAGCT GTGATTCTGA AAAGACTAGA 
GAACAACTGA GTTCTAAGAT AGATTCTAAA AGCAATTATG ATAAGCAGAA GAGTAAATCT 
TTGAATGCTA CACAAAAGGT AGCTTTGATT GAGTTAACAG AAATGCTGAA ATCCCTTTTC 
CAAGATGGTT ATACAGCCTT AATGGACCAG CGTTACCGTG GTGCCGAACA GGCCTTTTCG 
CAGTTGTTAA ACATTTTAGA TCCCAAGGAA TTAAAGCAAA TGAATCTTGC CATCACTGAC 
TACCTTGTCA TAATATATGG ATATGCCACT GCTCTTCTTG GAATAGGACA GCCTGAGGGA 
TTATCTGAAG CCGAAAACCA GTTTAAAAGA ATCCTTGAGC AGTACTCCAG TGAGGGATAT 
GATTGCTTGG CATATTATGG AATCGGGAAG GTTTATTTGA GAAAAAACAA ATTTTCAGAT 
GCTATTTATC ATTTTGAGAA ATCAAAAACC ATGATTAAAT TTATTCCTGG AGTATTAGTG 
TGGCCCACCA CTTCTGAGAT TATTGAAGAG TCTCAGTCTG AGAGATTACA ATCAATGTTA 
GAGAATTATA TAGAGGAATG TAAGTTCCCT CCAGAACCAG ATGCTGTGTG TTCCTATCAA 
AATTGCCATG GACATTCCAA AATCCAGATA TATTTAACTG ATCCAGATTT TAAGGGCTTT 
ATACGTATCA ACTGCTGCCA ATTGTGTAAA ATAGAATTTC ACATTAGCTG CTGGAAGAAG 
TTAAAAACAA CTTTCAATGA TAAAAATGAC AAGGATTTTC TTCAAGACCC ATGTCTTACC 
CCTGACTGTG GAGGAATCAT TTCCAAGATT ATTATCTTTG GTTCAACAGG CCAAATTAAA 
TGTGAATTTG AACATAAAGT TGTGAAAGAA AGGGATCCAC CGAGACCTGT AGTGAAACAG 
AAGTGTTCCA GCCTAGGGAA ACTAAGAATT AAAGAAGATA AAAAATCAAA GAGAAAGCTT 
CTAAAAAAAG AAGCAAAATC TTTAGCAAAC GAAAGAATGG AAGAGAAATC AAGTGACAAT 
AACTCATCTA AACACAGTGT CCATAAAGGC TGTGTTCAAA GTTGCCAAGT TATTGGTGAT 
AGAGTTCTGC ATTGTATAAA GCAAAATGCT GAAAAAATTA AATTTGGGGT ACAAGACACA 
TCTAAACTTC TGAGTGAATT GCTTTCTTGG TGGGTAATAA GCAAGGAGGA TTATACTGAA 
TGTTCTGCTA GCCATCTACC TAATGAAACC ATGGAGCGCC TTCTCTGTCA CTTGTTCCAG 
AACCAGAACA GAGTAAAGAC AAGAATTTTT TTACATGTAT TGAGTGAGAT TGAAGTTGTG 
GTTGACCCCA AACTACATGA GTGGATTCAG AAACTCAACA ATTTTGGCTT GGATGCCACA 
GAAGCTTTTT TTTCCCGTTA TGGAGATTCT ATTAGAGAGC TCAAATGCAA CATCATCACT 
GATCTTTGGA ATGAGAAATA TGGTCATAAA CTAGACCTTG TGCTCCCCAA TTCTGTAGAT 
GGAGAAATTC TTGAATGCTT TCATTTGTCA TCCTTGAAGG AAGCTCGCTG CCTAATATGG 
CTATTAGAAG AGAATAGGGA AAAATTTCCT ACTCTACATC ATATTTTAGA TGAATTTTTT 
GATATAATGG ATGGGCCCTG TACTATATTA AGGAAACAAG AAAATGAAGA TGCCTCAACT 
GTTATAAAGG TAAAAAACAA AAACCGGAAA AAGAAGCAAA AAGGATCAAA GCCTGTTTTC 
CCGCTGTCTG GAGTTGGAGG AATAATACAG GATGATGACA TTATTTTTAC AGATGAAAAT 
CCTCTATCTT TTTTGAATTC TTGGGATCCG TTTGTTGTTC CTGCTAACCT TCAAAATGAG 
ATAGAAGAAT TTGAAGCACT CTATGAAAAA AAGATTTCTA ATAGTCATCA TTATCAGAGA 
CTATTGGATA ATAATCCAGA TCCAAAATGT GAAACACTTT ATGACTATTT CTCTCAAATA 
CTGGAGGAAC ATGGTCCCCT GGAAATTACA GATGAGTTAT TGATTGGCGA ATATGAACAC 
TTCCCTGAAG AAACTCGGAA AGTTGTGGAA GAAGCAGGGG GATTGAAATC TTTTCTCCTG 
AGATCTCTTC GTTTTGTTAT GTTTAACGAC TTCATTGGAC TGATGAAAGA TGCAGTTGTC 
CTGAAGGAAA AGGAAGATTA CATGTTCAGT CCATATTTTG ACGAACACTC CTCACCAGTC 
AAACCATTAT TGAATCCAGC TGCAAAGGAA TTCAAGCCAT TCTCCCATAA CTGTAGTTCT 
AATGTGCCAA TGTCTGAAAC TATTAAAGAA AACAATCAGA CTGAAAAATA TGTGGACCCA 
GGACATCCTT CAGCTTTTCC TTTTAATTAT AGTGCTGATG TTCGTTATCA CGGATTTCAC 
ATCAATGAAG AATCGATGTT TTGTTTAAAT CTATTACCAT ATTCACCTGC CCATTTAAAT 
TTCTACACAG CTCCTGATTA TGAACTGGAA AAAATATTAC CAGTAGAGGC AGATAGGACA 
ACTGTTGCTG ACACTGCTAG ACCAAATAAT GATGATGAAT ATACTGATCA CAATGCTCCT 
CATTTTGATT CAGAAAAAGT AAAAGAGAGA GTCTTAGAAA GAGAGAACAT TTCTGGAAGG 
ATGGTTCTTA GTGAAACAGA GATATCAGCG AAATTAGACA ACTGCATTTA TAGAGATGAT 
AATACTCAAC TCCTGGGTAA CAGTAATGCT GGTCATTATA TTTCTACTAA TAAAATGAAA 
ATAGCGACTA AAGATATGAC ATTGGTGAGA AAAAATCCAG CTACACGAAT GATTGCTGTT 
CAGGTAAATC AGGACTTAAC ACATCAGGAG GTTAATACCA TGCCTTTTAA TCCATTTGAA 
ACCCAACATG GGGATATTCT GCGAATGGAA AAAGAACACC AGGTATTACA AGAGCAACTG 
AGAGAAGCAA ATGAAAAATA TACACAGTTA AAACACAGGA GTTCAGAAGA GATCAAAGAT 
TTGGAAGAAC AATTGAAAGA ACTTTCTGAA AAGAATAAGA TCTCAAAGAC TGAATTGGAA 
TGGATGCACC AGGACCTGGA AAAAGAACTG AAAAAATGGC AACAGGAAAA AAAAGAAAAT 
CAGGAAAAAT TGAAAGGCCT AAAGAGTAAA ATAAAGAAGT TTATAGAGAG CAATGAGAAC 
TGCTTAAGGA CGGTCAAGGA AAAAGATCAG CAGTATGAAT TGCATTTGAA TAAATTCCTG 
GAGATCAGTA ATAAATTTGA AAATGAGAAA GTGAAAATGG AGGAACATAT AAAGAAGGGA 
AAAGATAATT ACCAAGAGAG TAATAAAAGA TCTGTGGCAG CAGAGGTATC GGTCCTGAAA 
AACTGGAAGG AAAAGGAACT GAGCAAGTTG TTCAGCAGAG TATCAGAAGT AGAAAAATAT 
CTTAAGCAAC TAAAGTTAAT GAACAGTGAT TCATCTGAAG TGAAGCCTGA GATAGCTTCT 
TGGGAGTTGT TTCTTTCAAA AATTAAAAGA ATAATTGAGG AAACAAAGGC TCAATTTGAA 
GATAGAATTC AGAAGATTCA AAGTGGCTCC CCACTAGTCG GACTTTCTAA AGTGCAAATT 
CCCGATCTTT CAACTCCTTC CAGTAGTTTA AGAACTCTAG TCAACCTTCC TATTAGCGAT 
CCAGCCATTG TAATGTATTC TCCACCAATT CATCCTAAAT TACTTCCTAA ATATCCACAC 
CCTGAAGGTC AAAGTTCAGC GAATCTTCCA CGTCCTGTAC AAACTGGAAA CCAAATGACG 
ACACGTAAAG TCTCAAGTCC GTATCCTGCC AGTGATAGCT CCAAGGGGAA ACTTGTGCAA 
AGAAATTACC TTGATCCAGC TCTTCCCACT CTGCCTTCAG TGACTACTAG GAATCCTGAT 
GTCCAAGTGA ATCACAGCCT AATAGCTGAC CGTCTGTCAC CTAGTATTCA AAAACAGCCT 
CCACAGAAGC CATTTGAAGC CATTTTTAGA AATCTGGCAA TGATTTTCCC CAACTACAGC 
AGTAGTGAGT TTCTTTCTTT TATAAAAGAA GTTCAGGCCA AAAACAGGGG CACGCTTTCA 
GGATTGACCT CGGATGAAAT CACCAGGAGA GTGACAGATC TCATCCTGGA TGAGCCGAGT 
AAGAACAAGG CTGGAAAAGA TAGGGCAGCG GTATTTGTGG CAAATGACCC TGGCCGAAGA 
TCTTCGGAAG GAAGCGTGTT GGTCTCTGGC ACCTCCAAGA CCAGACCTGT TTCTAGCAAA 
AGCCCCAGCC ACCCACCTCG AGGCCAGCCC TGGGTGATAG TTGGGGGGCC AACCAAGACC 
AAGTGGCAGA AATCGAAAGA GCCCACTACA TCCGATGAAG ATCCCTGTAT CATCTGTCAT 
GACGAACTAA ATCAGGGCGT CCTTTGCAAA CTGGAATGTG GGCATCAGTT TCACAAACAG 
TGTATTGACC CCTGGCTAAA AGAACAAAAT ACATGTCCAA CCTGCCGGAA ACATATCCTG 
CTACCAGAGG ACTTTCCAGC TCTTTCTTCA GGAAGGAAGC CTACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028902374.1
CDS	108..5915
Translation	MEDWWTSNKEIISSGYVRFNKYCCEQGRANSKRCSSSKPKSAPDICKTWCSKPLQVLQDYCDIIKIYIFWPLLFQLEHMNYIDFSKSVHSELNLKKLQQIELLEEIVDLGKKVANDQFFIGGILKIGSKIEDRSPVMDETCNWVKYTGEFKVLHKLEKFGDYCWPMLEIFFTEYKYYITKVALEDCNLVEEFGSQNCDSCTRKGEMMKERGNEEFSQKKYDLAILFYTRAINFCPENHLLYSNRALCFLRIGQYKRALADGKRATILKHNWPKGHYRFCDALSMMGEHEQALEANEKAQKLCRNNPEGMKDLIQQNIKLRKEMEEIKGAKQSKFRMKKSLHEKKGPGFCPSHNPKETDNEKRRFISESKDHSCHQKRDIKVAPDVLNSEKQESPSEFPAGHNQKNKGKAKTSSCDSEKTREQLSSKIDSKSNYDKQKSKSLNATQKVALIELTEMLKSLFQDGYTALMDQRYRGAEQAFSQLLNILDPKELKQMNLAITDYLVIIYGYATALLGIGQPEGLSEAENQFKRILEQYSSEGYDCLAYYGIGKVYLRKNKFSDAIYHFEKSKTMIKFIPGVLVWPTTSEIIEESQSERLQSMLENYIEECKFPPEPDAVCSYQNCHGHSKIQIYLTDPDFKGFIRINCCQLCKIEFHISCWKKLKTTFNDKNDKDFLQDPCLTPDCGGIISKIIIFGSTGQIKCEFEHKVVKERDPPRPVVKQKCSSLGKLRIKEDKKSKRKLLKKEAKSLANERMEEKSSDNNSSKHSVHKGCVQSCQVIGDRVLHCIKQNAEKIKFGVQDTSKLLSELLSWWVISKEDYTECSASHLPNETMERLLCHLFQNQNRVKTRIFLHVLSEIEVVVDPKLHEWIQKLNNFGLDATEAFFSRYGDSIRELKCNIITDLWNEKYGHKLDLVLPNSVDGEILECFHLSSLKEARCLIWLLEENREKFPTLHHILDEFFDIMDGPCTILRKQENEDASTVIKVKNKNRKKKQKGSKPVFPLSGVGGIIQDDDIIFTDENPLSFLNSWDPFVVPANLQNEIEEFEALYEKKISNSHHYQRLLDNNPDPKCETLYDYFSQILEEHGPLEITDELLIGEYEHFPEETRKVVEEAGGLKSFLLRSLRFVMFNDFIGLMKDAVVLKEKEDYMFSPYFDEHSSPVKPLLNPAAKEFKPFSHNCSSNVPMSETIKENNQTEKYVDPGHPSAFPFNYSADVRYHGFHINEESMFCLNLLPYSPAHLNFYTAPDYELEKILPVEADRTTVADTARPNNDDEYTDHNAPHFDSEKVKERVLERENISGRMVLSETEISAKLDNCIYRDDNTQLLGNSNAGHYISTNKMKIATKDMTLVRKNPATRMIAVQVNQDLTHQEVNTMPFNPFETQHGDILRMEKEHQVLQEQLREANEKYTQLKHRSSEEIKDLEEQLKELSEKNKISKTELEWMHQDLEKELKKWQQEKKENQEKLKGLKSKIKKFIESNENCLRTVKEKDQQYELHLNKFLEISNKFENEKVKMEEHIKKGKDNYQESNKRSVAAEVSVLKNWKEKELSKLFSRVSEVEKYLKQLKLMNSDSSEVKPEIASWELFLSKIKRIIEETKAQFEDRIQKIQSGSPLVGLSKVQIPDLSTPSSSLRTLVNLPISDPAIVMYSPPIHPKLLPKYPHPEGQSSANLPRPVQTGNQMTTRKVSSPYPASDSSKGKLVQRNYLDPALPTLPSVTTRNPDVQVNHSLIADRLSPSIQKQPPQKPFEAIFRNLAMIFPNYSSSEFLSFIKEVQAKNRGTLSGLTSDEITRRVTDLILDEPSKNKAGKDRAAVFVANDPGRRSSEGSVLVSGTSKTRPVSSKSPSHPPRGQPWVIVGGPTKTKWQKSKEPTTSDEDPCIICHDELNQGVLCKLECGHQFHKQCIDPWLKEQNTCPTCRKHILLPEDFPALSSGRKPT

Target ORF information:

RefSeq Version	XM_029046541.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029046541.1

ORF Insert Sequence:

ATGGAAGACT GGTGGACCTC TAATAAGGAA ATTATTAGCT CCGGTTATGT TCGTTTTAAT 
AAATATTGCT GTGAACAGGG TCGTGCAAAT TCTAAAAGAT GTTCTTCAAG CAAGCCAAAA 
TCAGCTCCTG ACATCTGCAA AACCTGGTGC AGCAAACCAC TTCAAGTCCT GCAAGATTAC 
TGTGATATCA TTAAAATATA CATCTTTTGG CCACTTCTCT TTCAACTTGA GCATATGAAT 
TATATAGATT TCAGCAAATC TGTCCATTCT GAGTTAAATT TGAAGAAATT GCAACAGATT 
GAACTGCTGG AAGAAATTGT GGATCTGGGT AAGAAAGTTG CGAATGATCA GTTTTTTATT 
GGTGGCATCC TGAAAATTGG CTCCAAAATA GAAGATAGGA GCCCTGTAAT GGATGAAACC 
TGTAATTGGG TAAAATACAC AGGCGAATTT AAAGTTCTAC ATAAATTAGA AAAATTTGGA 
GATTACTGTT GGCCAATGTT AGAAATTTTC TTTACTGAAT ACAAATATTA TATAACCAAA 
GTTGCCCTGG AAGACTGCAA TTTAGTTGAA GAGTTTGGAT CTCAGAACTG CGATTCCTGT 
ACAAGGAAAG GAGAAATGAT GAAAGAGAGA GGAAATGAAG AATTTTCCCA AAAGAAGTAT 
GATTTGGCAA TCCTTTTCTA CACCAGAGCG ATTAACTTTT GCCCTGAAAA TCACCTTCTT 
TACAGTAACA GAGCTCTTTG TTTTCTTCGA ATTGGACAAT ACAAGAGAGC CCTTGCTGAT 
GGAAAAAGAG CTACTATTCT AAAGCATAAT TGGCCAAAGG GTCATTACCG TTTCTGTGAT 
GCACTCTCAA TGATGGGGGA ACATGAACAG GCCCTAGAAG CAAATGAAAA GGCTCAAAAA 
CTCTGCAGGA ATAATCCTGA AGGAATGAAG GATCTTATTC AGCAGAACAT AAAGTTAAGA 
AAAGAAATGG AAGAAATAAA AGGTGCAAAA CAAAGTAAGT TTCGAATGAA GAAATCACTT 
CATGAGAAAA AAGGGCCTGG GTTTTGTCCT TCACATAATC CAAAGGAAAC AGACAATGAA 
AAAAGAAGGT TCATATCGGA ATCAAAAGAT CATAGTTGTC ATCAGAAGCG GGATATAAAG 
GTGGCTCCTG ATGTCCTGAA TTCAGAAAAA CAAGAGTCTC CTTCGGAGTT TCCAGCAGGT 
CATAATCAGA AAAATAAAGG AAAAGCAAAA ACTAGTAGCT GTGATTCTGA AAAGACTAGA 
GAACAACTGA GTTCTAAGAT AGATTCTAAA AGCAATTATG ATAAGCAGAA GAGTAAATCT 
TTGAATGCTA CACAAAAGGT AGCTTTGATT GAGTTAACAG AAATGCTGAA ATCCCTTTTC 
CAAGATGGTT ATACAGCCTT AATGGACCAG CGTTACCGTG GTGCCGAACA GGCCTTTTCG 
CAGTTGTTAA ACATTTTAGA TCCCAAGGAA TTAAAGCAAA TGAATCTTGC CATCACTGAC 
TACCTTGTCA TAATATATGG ATATGCCACT GCTCTTCTTG GAATAGGACA GCCTGAGGGA 
TTATCTGAAG CCGAAAACCA GTTTAAAAGA ATCCTTGAGC AGTACTCCAG TGAGGGATAT 
GATTGCTTGG CATATTATGG AATCGGGAAG GTTTATTTGA GAAAAAACAA ATTTTCAGAT 
GCTATTTATC ATTTTGAGAA ATCAAAAACC ATGATTAAAT TTATTCCTGG AGTATTAGTG 
TGGCCCACCA CTTCTGAGAT TATTGAAGAG TCTCAGTCTG AGAGATTACA ATCAATGTTA 
GAGAATTATA TAGAGGAATG TAAGTTCCCT CCAGAACCAG ATGCTGTGTG TTCCTATCAA 
AATTGCCATG GACATTCCAA AATCCAGATA TATTTAACTG ATCCAGATTT TAAGGGCTTT 
ATACGTATCA ACTGCTGCCA ATTGTGTAAA ATAGAATTTC ACATTAGCTG CTGGAAGAAG 
TTAAAAACAA CTTTCAATGA TAAAAATGAC AAGGATTTTC TTCAAGACCC ATGTCTTACC 
CCTGACTGTG GAGGAATCAT TTCCAAGATT ATTATCTTTG GTTCAACAGG CCAAATTAAA 
TGTGAATTTG AACATAAAGT TGTGAAAGAA AGGGATCCAC CGAGACCTGT AGTGAAACAG 
AAGTGTTCCA GCCTAGGGAA ACTAAGAATT AAAGAAGATA AAAAATCAAA GAGAAAGCTT 
CTAAAAAAAG AAGCAAAATC TTTAGCAAAC GAAAGAATGG AAGAGAAATC AAGTGACAAT 
AACTCATCTA AACACAGTGT CCATAAAGGC TGTGTTCAAA GTTGCCAAGT TATTGGTGAT 
AGAGTTCTGC ATTGTATAAA GCAAAATGCT GAAAAAATTA AATTTGGGGT ACAAGACACA 
TCTAAACTTC TGAGTGAATT GCTTTCTTGG TGGGTAATAA GCAAGGAGGA TTATACTGAA 
TGTTCTGCTA GCCATCTACC TAATGAAACC ATGGAGCGCC TTCTCTGTCA CTTGTTCCAG 
AACCAGAACA GAGTAAAGAC AAGAATTTTT TTACATGTAT TGAGTGAGAT TGAAGTTGTG 
GTTGACCCCA AACTACATGA GTGGATTCAG AAACTCAACA ATTTTGGCTT GGATGCCACA 
GAAGCTTTTT TTTCCCGTTA TGGAGATTCT ATTAGAGAGC TCAAATGCAA CATCATCACT 
GATCTTTGGA ATGAGAAATA TGGTCATAAA CTAGACCTTG TGCTCCCCAA TTCTGTAGAT 
GGAGAAATTC TTGAATGCTT TCATTTGTCA TCCTTGAAGG AAGCTCGCTG CCTAATATGG 
CTATTAGAAG AGAATAGGGA AAAATTTCCT ACTCTACATC ATATTTTAGA TGAATTTTTT 
GATATAATGG ATGGGCCCTG TACTATATTA AGGAAACAAG AAAATGAAGA TGCCTCAACT 
GTTATAAAGG TAAAAAACAA AAACCGGAAA AAGAAGCAAA AAGGATCAAA GCCTGTTTTC 
CCGCTGTCTG GAGTTGGAGG AATAATACAG GATGATGACA TTATTTTTAC AGATGAAAAT 
CCTCTATCTT TTTTGAATTC TTGGGATCCG TTTGTTGTTC CTGCTAACCT TCAAAATGAG 
ATAGAAGAAT TTGAAGCACT CTATGAAAAA AAGATTTCTA ATAGTCATCA TTATCAGAGA 
CTATTGGATA ATAATCCAGA TCCAAAATGT GAAACACTTT ATGACTATTT CTCTCAAATA 
CTGGAGGAAC ATGGTCCCCT GGAAATTACA GATGAGTTAT TGATTGGCGA ATATGAACAC 
TTCCCTGAAG AAACTCGGAA AGTTGTGGAA GAAGCAGGGG GATTGAAATC TTTTCTCCTG 
AGATCTCTTC GTTTTGTTAT GTTTAACGAC TTCATTGGAC TGATGAAAGA TGCAGTTGTC 
CTGAAGGAAA AGGAAGATTA CATGTTCAGT CCATATTTTG ACGAACACTC CTCACCAGTC 
AAACCATTAT TGAATCCAGC TGCAAAGGAA TTCAAGCCAT TCTCCCATAA CTGTAGTTCT 
AATGTGCCAA TGTCTGAAAC TATTAAAGAA AACAATCAGA CTGAAAAATA TGTGGACCCA 
GGACATCCTT CAGCTTTTCC TTTTAATTAT AGTGCTGATG TTCGTTATCA CGGATTTCAC 
ATCAATGAAG AATCGATGTT TTGTTTAAAT CTATTACCAT ATTCACCTGC CCATTTAAAT 
TTCTACACAG CTCCTGATTA TGAACTGGAA AAAATATTAC CAGTAGAGGC AGATAGGACA 
ACTGTTGCTG ACACTGCTAG ACCAAATAAT GATGATGAAT ATACTGATCA CAATGCTCCT 
CATTTTGATT CAGAAAAAGT AAAAGAGAGA GTCTTAGAAA GAGAGAACAT TTCTGGAAGG 
ATGGTTCTTA GTGAAACAGA GATATCAGCG AAATTAGACA ACTGCATTTA TAGAGATGAT 
AATACTCAAC TCCTGGGTAA CAGTAATGCT GGTCATTATA TTTCTACTAA TAAAATGAAA 
ATAGCGACTA AAGATATGAC ATTGGTGAGA AAAAATCCAG CTACACGAAT GATTGCTGTT 
CAGGTAAATC AGGACTTAAC ACATCAGGAG GTTAATACCA TGCCTTTTAA TCCATTTGAA 
ACCCAACATG GGGATATTCT GCGAATGGAA AAAGAACACC AGGTATTACA AGAGCAACTG 
AGAGAAGCAA ATGAAAAATA TACACAGTTA AAACACAGGA GTTCAGAAGA GATCAAAGAT 
TTGGAAGAAC AATTGAAAGA ACTTTCTGAA AAGAATAAGA TCTCAAAGAC TGAATTGGAA 
TGGATGCACC AGGACCTGGA AAAAGAACTG AAAAAATGGC AACAGGAAAA AAAAGAAAAT 
CAGGAAAAAT TGAAAGGCCT AAAGAGTAAA ATAAAGAAGT TTATAGAGAG CAATGAGAAC 
TGCTTAAGGA CGGTCAAGGA AAAAGATCAG CAGTATGAAT TGCATTTGAA TAAATTCCTG 
GAGATCAGTA ATAAATTTGA AAATGAGAAA GTGAAAATGG AGGAACATAT AAAGAAGGGA 
AAAGATAATT ACCAAGAGAG TAATAAAAGA TCTGTGGCAG CAGAGGTATC GGTCCTGAAA 
AACTGGAAGG AAAAGGAACT GAGCAAGTTG TTCAGCAGAG TATCAGAAGT AGAAAAATAT 
CTTAAGCAAC TAAAGTTAAT GAACAGTGAT TCATCTGAAG TGAAGCCTGA GATAGCTTCT 
TGGGAGTTGT TTCTTTCAAA AATTAAAAGA ATAATTGAGG AAACAAAGGC TCAATTTGAA 
GATAGAATTC AGAAGATTCA AAGTGGCTCC CCACTAGTCG GACTTTCTAA AGTGCAAATT 
CCCGATCTTT CAACTCCTTC CAGTAGTTTA AGAACTCTAG TCAACCTTCC TATTAGCGAT 
CCAGCCATTG TAATGTATTC TCCACCAATT CATCCTAAAT TACTTCCTAA ATATCCACAC 
CCTGAAGGTC AAAGTTCAGC GAATCTTCCA CGTCCTGTAC AAACTGGAAA CCAAATGACG 
ACACGTAAAG TCTCAAGTCC GTATCCTGCC AGTGATAGCT CCAAGGGGAA ACTTGTGCAA 
AGAAATTACC TTGATCCAGC TCTTCCCACT CTGCCTTCAG TGACTACTAG GAATCCTGAT 
GTCCAAGTGA ATCACAGCCT AATAGCTGAC CGTCTGTCAC CTAGTATTCA AAAACAGCCT 
CCACAGAAGC CATTTGAAGC CATTTTTAGA AATCTGGCAA TGATTTTCCC CAACTACAGC 
AGTAGTGAGT TTCTTTCTTT TATAAAAGAA GTTCAGGCCA AAAACAGGGG CACGCTTTCA 
GGATTGACCT CGGATGAAAT CACCAGGAGA GTGACAGATC TCATCCTGGA TGAGCCGAGT 
AAGAACAAGG CTGGAAAAGA TAGGGCAGCG GTATTTGTGG CAAATGACCC TGGCCGAAGA 
TCTTCGGAAG GAAGCGTGTT GGTCTCTGGC ACCTCCAAGA CCAGACCTGT TTCTAGCAAA 
AGCCCCAGCC ACCCACCTCG AGGCCAGCCC TGGGTGATAG TTGGGGGGCC AACCAAGACC 
AAGTGGCAGA AATCGAAAGA GCCCACTACA TCCGATGAAG ATCCCTGTAT CATCTGTCAT 
GACGAACTAA ATCAGGGCGT CCTTTGCAAA CTGGAATGTG GGCATCAGTT TCACAAACAG 
TGTATTGACC CCTGGCTAAA AGAACAAAAT ACATGTCCAA CCTGCCGGAA ACATATCCTG 
CTACCAGAGG ACTTTCCAGC TCTTTCTTCA GGAAGGAAGC CTACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_007667267.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_007665457.1 Ornithorhynchus anatinus tetratricopeptide repeat domain 3 (TTC3), mRNA.

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CloneID	OOc07464
Clone ID Related Accession (Same CDS sequence)	XM_007667267.1
Accession Version	XM_007667267.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-14
Organism	Ornithorhynchus anatinus(platypus)
Product	E3 ubiquitin-protein ligase TTC3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001794129.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ornithorhynchus anatinus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 5% of CDS bases ##RefSeq-Attributes-END##

ATGGAAGACT GGTGGACCTC TAATAAGGAA ATTATTAGCT CCGGTTATGT TCGTTTTAAT 
AAATATTGCT GTGAACAGGG TCGTGCAAAT TCTAAAAGAT GTTCTTCAAG CAAGCCAAAA 
TCAGCTCCTG ACATCTGCAA AACCTGGTGC AGCAAACCAC TTCAAGTCCT GCAAGATTAC 
TGTGATATCA TTAAAATATA CATCTTTTGG CCACTTCTCT TTCAACTTGA GCATATGAAT 
TATATAGATT TCAGCAAATC TGTCCATTCT GAGTTAAATT TGAAGAAATT GCAACAGATT 
GAACTGCTGG AAGAAATTGT GGATCTGGGT AAGAAAGTTG CGAATGATCA GTTTTTTATT 
GGTGGCATCC TGAAAATTGG CTCCAAAATA GAAGATAGGA GCCCTGTAAT GGATGAAACC 
TGTAATTGGG TAAAATACAC AGGCGAATTT AAAGTTCTAC ATAAATTAGA AAAATTTGGA 
GATTACTGTT GGCCAATGTT AGAAATTTTC TTTACTGAAT ACAAATATTA TATAACCAAA 
GTTGCCCTGG AAGACTGCAA TTTAGTTGAA GAGTTTGGAT CTCAGAACTG CGATTCCTGT 
ACAAGGAAAG GAGAAATGAT GAAAGAGAGA GGAAATGAAG AATTTTCCCA GAAGAAGTAT 
GATTTGGCAA TCCTTTTCTA CACCAGAGCG ATTAACTTTT GCCCTGAAAA TCACCTTCTT 
TACAGTAACA GAGCTCTTTG TTTTCTTCGA ATTGGACAAT ACAAGAGAGC CCTTGCTGAT 
GGAAAAAGAG CTACTATTCT AAAGCATAAT TGGCCAAAGG GTCATTACCG TTTCTGTGAT 
GCACTCTCAA TGATGGGGGA ACATGAACAG GCCCTAGAAG CAAATGAAAA GGCTCAAAAA 
CTCTGCAGGA ATAATCCTGA AGGAATGAAG GATCTTATTC AGCAGAACAT AAAGTTAAGA 
AAAGAAATGG AAGAAATAAA AGGTGCAAAA CAAAGTAAGT TTCGAATGAA GAAATCACTT 
CATGAGAAAA AATTAACTTC AGATTGTGGT TCTAGAGGGC CTGGGTTTTG TCCTTCACAT 
AATCCAAAGG AAACAGACAA TGAAAAAAGA AGGTTCATAT CGGAATCAAA AGATCATAGT 
TGTCATCAGA AGCGGGATAT AAAGGTGGCT CCTGATGTCC TGAATTCAGA AAAACAAGAG 
TCTCCTTCGG AGTTTCCAGC AGGTCATAAT CAGAAAAATA AAGGAAAAGC AAAAACTAGT 
AGCTGTGATT CTGAAAAGAC TAGAGAACAA CTGAGTTCTA AGATAGATTC TAAAAGCAAT 
TATGATAAGC AGAAGAGTAA ATCTTTGAAT GCTACACAAA AGGTAGCTTT GATTGAGTTA 
ACAGAAATGC TGAAATCCCT TTTCCAAGAT GGTTATACAG CCTTAATGGA CCAGCGTTAC 
CGTGGTGCCG AACAGGCCTT TTCGCAGTTG TTAAACATTT TAGATCCCAA GGAATTAAAG 
CAAATGAATC TTGCCATCAC TGACTACCTT GTCATAATAT ATGGATATGC CACTGCTCTT 
CTTGGAATAG GACAGCCTGA GGGATTATCT GAAGCCGAAA ACCAGTTTAA AAGAATCCTT 
GAGCAGTACT CCAGTGAGGG ATATGATTGC TTGGCATATT ATGGAATCGG GAAGGTTTAT 
TTGAGAAAAA ACAAATTTTC AGATGCTATT TATCATTTTG AGAAATCAAA AACCATGATT 
AAATTTATTC CTGGAGTATT AGTGTGGCCC ACCACTTCTG AGATTATTGA AGAGTCTCAG 
TCTGAGAGAT TACAATCAAT GTTAGAGAAT TATATAGAGG AATGTAAGTT CCCTCCAGAA 
CCAGATGCTG TGTGTTCCTA TCAAAATTGC CATGGACATT CCAAAATCCA GATATATTTA 
ACTGATCCAG ATTTTAAGGG CTTTATACGT ATCAACTGCT GCCAATTGTG TAAAATAGAA 
TTTCACATTA GCTGCTGGAA GAAGTTAAAA ACAACTTTCA ATGATAAAAA TGACAAGGAT 
TTTCTTCAAG ACCCATGTCT TACCCCTGAC TGTGGAGGAA TCATTTCCAA GATTATTATC 
TTTGGTTCAA CAGGCCAAAT TAAATGTGAA TTTGAACATA AAGTTGTGAA AGAAAGGGAT 
CCACCGAGAC CTGTAGTGAA ACAGAAGTGT TCCAGCCTAG GGAAACTAAG AATTAAAGAA 
GATAAAAAAT CAAAGAGAAA GCTTCTAAAA AAAGAAGCAA AATCTTTAGC AAACGAAAGA 
ATGGAAGAGA AATCAAGTGA CAATAACTCA TCTAAACACA GTGTCCATAA AGGCTGTGTT 
CAAAGTTGCC AAGTTATTGG TGATAGAGTT CTGCATTGTA TAAAGCAAAA TGCTGAAAAA 
ATTAAATTTG GGGTACAAGA CACATCTAAA CTTCTGAGTG AATTGCTTTC TTGGTGGGTA 
ATAAGCAAGG AGGATTATAC TGAATGTTCT GCCAGCCATC TACCTAATGA AACCATGGAG 
CGCCTTCTCT GTCACTTGTT CCAGAACCAG AACAGAGTAA AGACAAGAAT TTTTTTACAT 
GTATTGAGTG AGATTGAAGT TGTGGTTGAC CCCAAACTAC ATGAGTGGAT TCAGAAACTC 
AACAATTTTG GCTTGGATGC CACAGAAGCT TTTTTTTCCC GTTATGGAGA TTCTATTAGA 
GAGCTCAAAT GCAACATCAT CACTGATCTT TGGAATGAGA AATATGGTCA TAAACTAGAC 
CTTGTGCTCC CCAATTCTGT AGATGGAGAA ATTCTTGAAT GCTTTCATTT GTCATCCTTG 
AAGGAAGCTC GCTGCCTAAT ATGGCTATTA GAAGAGAATA GGGAAAAATT TCCTACTCTA 
CATCATATTT TAGATGAATT TTTTGATATA ATGGATGGGC CCTGTACTAT ATTAAGGAAA 
CAAGAAAATG AAGATGCCTC AACTGTTATA AAGGTAAAAA ACAAAAACCG GAAAAAGAAG 
CAAAAAGGAT CAAAGCCTGT TTTCCCGCTG TCTGGAGTTG GAGGAATAAT ACAGGATGAT 
GACATTATTT TTACAGATGA AAATCCTCTA TCTTTTTTGA ATTCTTGGGA TCCGTTTGTT 
GTTCCTGCTA ACCTTCAAAA TGAGATAGAA GAATTTGAAG CACTCTATGA AAAAAAGATT 
TCTAATAGTC ATCATTATCA GAGACTATTG GATAATAATC CAGATCCAAA ATGTGAAACA 
CTTTATGACT ATTTCTCTCA AATACTGGAG GAACATGGTC CCCTGGAAAT TACAGATGAG 
TTATTGATTG GCGAATATGA ACACTTCCCT GAAGAAACTC GGAAAGTTGT GGAAGAAGCA 
GGGGGATTGA AATCTTTTCT CCTGAGATCT CTTCGTTTTG TTATGTTTAA CGACTTCATT 
GGACTGATGA AAGATGCAGT TGTCCTGAAG GAAAAGGAAG ATTACATGTT CAGTCCATAT 
TTTGACGAAC ACTCCTCACC AGTCAAACCA TTATTGAATC CAGCTGCAAA GGAATTCAAG 
CCATTCTCCC ATAACTGTAG TTCTAATGTG CCAATGTCTG AAACTATTAA AGAAAACAAT 
CAGACTGAAA AATATGTGGA CCCAGGACAT CCTTCAGCTT TTCCTTTTAA TTATAGTGCT 
GATGTTCGTT ATCACGGATT TCACATCAAT GAAGAATCAA TGTTTTGTTT AAATCTATTA 
CCATATTCAC CTGCCCATTT AAATTTCTAC ACACCTCCTG ATTATGAACT GGAAAAAATA 
TTACCAGTAG AGGCAGATAG GACAACTGTT GCTGACACTG CTAGACCAAA TAATGATGAT 
GAATATACTG ATCACAATGC TCCTCATTTT GATTCAGAAA AAGTAAAAGA GAGAGTCTTA 
GAAAGAGAGA ACATTTCTGG AAGGATGGTT TTTAGTGAAA CAGAGATATC AGCGAAATTA 
GACAACTGCA TTTATAGAGA TGATAATACT CAACTCCTGG GTAACAGTAA TGCTGGTCAT 
TATATTTCTA CTAATAAAAT GAAAATAGCG ACTAAAGATA TGGCATTGGT GAGAAAAAAT 
CCAGCTACAC GAATGATTGC TGTTCAGGTA AATCAGGACT TAACACATCA GGAGGTTAAT 
ACCATGCCTT TTAATCCATT TGAAACCCAA CATGGGGATA TTCTGCGAAT GGAAAAAGAA 
CACCAGGTAT TACAAGAGCA ACTGAGAGAA GCAAATGAAA AATATACACA GTTAAAACAC 
AGGAGTTCAG AAGAGATCAA AGATTTGGAA GAACAATTGA AAGAACTTTC TGAAAAGAAT 
AAGATCTCAA AGACTGAATT GGAATGGATG CACCAGGACC TGGAAAAAGA ACTGAAAAAA 
TGGCAACAGG AAAAAAAAGA AAATCAGGAA AAATTGAAAG GCCTAAAGAG TAAAATAAAG 
AAGTTTATAG AGAGCAATGA GAAGAATTTG AGGACTTTTT TAATGACTAA TGTTAGTGCT 
GTAAATCAGG ACTTAACACA TCAGGAGGTT AATACCATGC CTTTTAATCC ATTTGAAACC 
CAACATGGGG ATATTCTGCG AATGGAAAAA GAACACCAGG TATTACAAGA GCAACTGAGA 
GAAGCAAATG AAAAATATAC ACAGTTAAAA CACAGGAGTT CAGAAGAGAT CAAAGATTTG 
GAAGAACAAT TGAAAGAACT TTCTGAAAAG AATAAGATCT CAAAGACTGA ATTGGAATGG 
ATGCACCAGG ACCTGGAAAA AGAACTGAAA AAATGGCAAC AGGAAAAAAA AGAAAATCAG 
GAAAAATTGA AAGGCCTAAA GAGTAAAATA AAGAAGTTTA TAGAGAGCAA TGAGAACTGC 
TTAAGGACGG TCAAGGAAAA AGATCAGCAG TATGAATTGC ATTTGAATAA ATTCCTGGAG 
ATCAGTAATA AATTTGAAAA TGAGAAAGTG AAAATGGAGG AACATATAAA GAAGGGAAAA 
GATAATTACC AAGAGAGTAA TAAAAGATCT GTGGCAGCAG AGGTATCGGT CCTGAAAAAC 
TGGAAGGAAA AGGAACTGAG CAAGTTGTTC AGCAGAGTAT CAGAAGTAGA AAAATATCTT 
AAGCAACTAA AGTTAATGAA CAGTGATTCA TCTGAAGTGA AGCCTGAGAT AGCTTCTTGG 
GAGTTGTTTC TTTCAAAAAT TAAAAGAATA ATTGAGGAAA CAAAGGCTCA GTTTGAAGAT 
AGAATTCAGA AGATTCAAAG TGGCTCCCCA CTAGTCGGAC TTTCTAAAGT GCAAATTCCC 
GATCTTTCAA CTCCTTCCAG TAGTTTAAGA ACTCTAGTCA ACCTTCCTAT TAGCGATCCA 
GCCATTGTAA TGTATTCTCC ACCAATTCAT CCTAAATTAC TTCCTAAATA TCCACACCCT 
GAAGGTCAAA GTTCAGCGAA TCTTCCACGT CCTGTACAAA CTGGAAACCA AATGACGACA 
CGTAAAGTCT CAAGTCCGTA TCCTGCCAGT GATAGCTCCA AGGGGAAACT TGTGCAAAGA 
AATTACCTTG ATCCAGCTCT TCCCACTCTG CCTTCAGTGA CTACTAGGAA TCCTGATGTC 
CAAGTGAATC ACAGCCTAAT AGCTGACCGT CTGTCACCTA GTATTCAAAA ACAGCCTCCA 
CAGAAGCCAT TTGAAGCCAT TTTTAGAAAT CTGGCAATGA TTTTCCCCAA CTACAGCAGT 
AGTGAGTTTC TTTCTTTTAT AAAAGAAGTT CAGGCCAAAA ACAGGGGCAC GCTTTCAGGA 
TTGACCTCGG ATGAAATCAC CAGGAGAGTG ACAGATCTCA TCCTGGATGA GCCGAGTAAG 
AACAAGGCTG GAAAAGATAG GGCAGCGGTA TTTGTGGCAA ATGACCCTGG CCGAAGATCT 
TCGGAAGGAA GCGTGTTGGT CTCTGGCACC TCCAAGACCA GACCTGTTTC TAGCAAAAGC 
CCCAGCCACC CACCTCGAGG CCAGCCCTGG GTGATAGTTG GGGGGCCAAC CAAGACCAAG 
TGGCAGAAAT CGAAAGAGCC CACTACATCC GATGAAGATC CCTGTATCAT CTGTCATGAC 
GAACTAAATC AGGGCGTCCT TTGCAAACTG GAATGTGGGC ATCAGTTTCA CAAACAGGGC 
CGGGCCGGGC CGGGCCGGGC TGCCGGTGTT TCCGAGGAGG GAGGCCTCGG CCGGGACGGA 
AGGACGGAAG GACGGACCCC GGTTTCCCCG GCGCGGCTGC CAACGGAGCG AGGTGCCCTC 
GTTGGCCTTA AACCTTCCCC CGCCCGCGCC CGCGGCTTAG CCCGGCCCGG AGGGCCGTCC 
TGGGTTCGAA TCCCGCTTCT CAGCCGGGGG CCTCCGGGCC GGTCATTTGC CTTCTCCGTC 
TGTGTCCGTG CCCTCCTCTG CCAGGTGCGG GTTGGTCCTC CTTCCCACTA CGGCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007665457.1
CDS	108..6527
Translation	MEDWWTSNKEIISSGYVRFNKYCCEQGRANSKRCSSSKPKSAPDICKTWCSKPLQVLQDYCDIIKIYIFWPLLFQLEHMNYIDFSKSVHSELNLKKLQQIELLEEIVDLGKKVANDQFFIGGILKIGSKIEDRSPVMDETCNWVKYTGEFKVLHKLEKFGDYCWPMLEIFFTEYKYYITKVALEDCNLVEEFGSQNCDSCTRKGEMMKERGNEEFSQKKYDLAILFYTRAINFCPENHLLYSNRALCFLRIGQYKRALADGKRATILKHNWPKGHYRFCDALSMMGEHEQALEANEKAQKLCRNNPEGMKDLIQQNIKLRKEMEEIKGAKQSKFRMKKSLHEKKLTSDCGSRGPGFCPSHNPKETDNEKRRFISESKDHSCHQKRDIKVAPDVLNSEKQESPSEFPAGHNQKNKGKAKTSSCDSEKTREQLSSKIDSKSNYDKQKSKSLNATQKVALIELTEMLKSLFQDGYTALMDQRYRGAEQAFSQLLNILDPKELKQMNLAITDYLVIIYGYATALLGIGQPEGLSEAENQFKRILEQYSSEGYDCLAYYGIGKVYLRKNKFSDAIYHFEKSKTMIKFIPGVLVWPTTSEIIEESQSERLQSMLENYIEECKFPPEPDAVCSYQNCHGHSKIQIYLTDPDFKGFIRINCCQLCKIEFHISCWKKLKTTFNDKNDKDFLQDPCLTPDCGGIISKIIIFGSTGQIKCEFEHKVVKERDPPRPVVKQKCSSLGKLRIKEDKKSKRKLLKKEAKSLANERMEEKSSDNNSSKHSVHKGCVQSCQVIGDRVLHCIKQNAEKIKFGVQDTSKLLSELLSWWVISKEDYTECSASHLPNETMERLLCHLFQNQNRVKTRIFLHVLSEIEVVVDPKLHEWIQKLNNFGLDATEAFFSRYGDSIRELKCNIITDLWNEKYGHKLDLVLPNSVDGEILECFHLSSLKEARCLIWLLEENREKFPTLHHILDEFFDIMDGPCTILRKQENEDASTVIKVKNKNRKKKQKGSKPVFPLSGVGGIIQDDDIIFTDENPLSFLNSWDPFVVPANLQNEIEEFEALYEKKISNSHHYQRLLDNNPDPKCETLYDYFSQILEEHGPLEITDELLIGEYEHFPEETRKVVEEAGGLKSFLLRSLRFVMFNDFIGLMKDAVVLKEKEDYMFSPYFDEHSSPVKPLLNPAAKEFKPFSHNCSSNVPMSETIKENNQTEKYVDPGHPSAFPFNYSADVRYHGFHINEESMFCLNLLPYSPAHLNFYTPPDYELEKILPVEADRTTVADTARPNNDDEYTDHNAPHFDSEKVKERVLERENISGRMVFSETEISAKLDNCIYRDDNTQLLGNSNAGHYISTNKMKIATKDMALVRKNPATRMIAVQVNQDLTHQEVNTMPFNPFETQHGDILRMEKEHQVLQEQLREANEKYTQLKHRSSEEIKDLEEQLKELSEKNKISKTELEWMHQDLEKELKKWQQEKKENQEKLKGLKSKIKKFIESNEKNLRTFLMTNVSAVNQDLTHQEVNTMPFNPFETQHGDILRMEKEHQVLQEQLREANEKYTQLKHRSSEEIKDLEEQLKELSEKNKISKTELEWMHQDLEKELKKWQQEKKENQEKLKGLKSKIKKFIESNENCLRTVKEKDQQYELHLNKFLEISNKFENEKVKMEEHIKKGKDNYQESNKRSVAAEVSVLKNWKEKELSKLFSRVSEVEKYLKQLKLMNSDSSEVKPEIASWELFLSKIKRIIEETKAQFEDRIQKIQSGSPLVGLSKVQIPDLSTPSSSLRTLVNLPISDPAIVMYSPPIHPKLLPKYPHPEGQSSANLPRPVQTGNQMTTRKVSSPYPASDSSKGKLVQRNYLDPALPTLPSVTTRNPDVQVNHSLIADRLSPSIQKQPPQKPFEAIFRNLAMIFPNYSSSEFLSFIKEVQAKNRGTLSGLTSDEITRRVTDLILDEPSKNKAGKDRAAVFVANDPGRRSSEGSVLVSGTSKTRPVSSKSPSHPPRGQPWVIVGGPTKTKWQKSKEPTTSDEDPCIICHDELNQGVLCKLECGHQFHKQGRAGPGRAAGVSEEGGLGRDGRTEGRTPVSPARLPTERGALVGLKPSPARARGLARPGGPSWVRIPLLSRGPPGRSFAFSVCVRALLCQVRVGPPSHYGP

Target ORF information:

RefSeq Version	XM_007667267.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus tetratricopeptide repeat domain 3 (TTC3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007667267.1

ORF Insert Sequence:

ATGGAAGACT GGTGGACCTC TAATAAGGAA ATTATTAGCT CCGGTTATGT TCGTTTTAAT 
AAATATTGCT GTGAACAGGG TCGTGCAAAT TCTAAAAGAT GTTCTTCAAG CAAGCCAAAA 
TCAGCTCCTG ACATCTGCAA AACCTGGTGC AGCAAACCAC TTCAAGTCCT GCAAGATTAC 
TGTGATATCA TTAAAATATA CATCTTTTGG CCACTTCTCT TTCAACTTGA GCATATGAAT 
TATATAGATT TCAGCAAATC TGTCCATTCT GAGTTAAATT TGAAGAAATT GCAACAGATT 
GAACTGCTGG AAGAAATTGT GGATCTGGGT AAGAAAGTTG CGAATGATCA GTTTTTTATT 
GGTGGCATCC TGAAAATTGG CTCCAAAATA GAAGATAGGA GCCCTGTAAT GGATGAAACC 
TGTAATTGGG TAAAATACAC AGGCGAATTT AAAGTTCTAC ATAAATTAGA AAAATTTGGA 
GATTACTGTT GGCCAATGTT AGAAATTTTC TTTACTGAAT ACAAATATTA TATAACCAAA 
GTTGCCCTGG AAGACTGCAA TTTAGTTGAA GAGTTTGGAT CTCAGAACTG CGATTCCTGT 
ACAAGGAAAG GAGAAATGAT GAAAGAGAGA GGAAATGAAG AATTTTCCCA GAAGAAGTAT 
GATTTGGCAA TCCTTTTCTA CACCAGAGCG ATTAACTTTT GCCCTGAAAA TCACCTTCTT 
TACAGTAACA GAGCTCTTTG TTTTCTTCGA ATTGGACAAT ACAAGAGAGC CCTTGCTGAT 
GGAAAAAGAG CTACTATTCT AAAGCATAAT TGGCCAAAGG GTCATTACCG TTTCTGTGAT 
GCACTCTCAA TGATGGGGGA ACATGAACAG GCCCTAGAAG CAAATGAAAA GGCTCAAAAA 
CTCTGCAGGA ATAATCCTGA AGGAATGAAG GATCTTATTC AGCAGAACAT AAAGTTAAGA 
AAAGAAATGG AAGAAATAAA AGGTGCAAAA CAAAGTAAGT TTCGAATGAA GAAATCACTT 
CATGAGAAAA AATTAACTTC AGATTGTGGT TCTAGAGGGC CTGGGTTTTG TCCTTCACAT 
AATCCAAAGG AAACAGACAA TGAAAAAAGA AGGTTCATAT CGGAATCAAA AGATCATAGT 
TGTCATCAGA AGCGGGATAT AAAGGTGGCT CCTGATGTCC TGAATTCAGA AAAACAAGAG 
TCTCCTTCGG AGTTTCCAGC AGGTCATAAT CAGAAAAATA AAGGAAAAGC AAAAACTAGT 
AGCTGTGATT CTGAAAAGAC TAGAGAACAA CTGAGTTCTA AGATAGATTC TAAAAGCAAT 
TATGATAAGC AGAAGAGTAA ATCTTTGAAT GCTACACAAA AGGTAGCTTT GATTGAGTTA 
ACAGAAATGC TGAAATCCCT TTTCCAAGAT GGTTATACAG CCTTAATGGA CCAGCGTTAC 
CGTGGTGCCG AACAGGCCTT TTCGCAGTTG TTAAACATTT TAGATCCCAA GGAATTAAAG 
CAAATGAATC TTGCCATCAC TGACTACCTT GTCATAATAT ATGGATATGC CACTGCTCTT 
CTTGGAATAG GACAGCCTGA GGGATTATCT GAAGCCGAAA ACCAGTTTAA AAGAATCCTT 
GAGCAGTACT CCAGTGAGGG ATATGATTGC TTGGCATATT ATGGAATCGG GAAGGTTTAT 
TTGAGAAAAA ACAAATTTTC AGATGCTATT TATCATTTTG AGAAATCAAA AACCATGATT 
AAATTTATTC CTGGAGTATT AGTGTGGCCC ACCACTTCTG AGATTATTGA AGAGTCTCAG 
TCTGAGAGAT TACAATCAAT GTTAGAGAAT TATATAGAGG AATGTAAGTT CCCTCCAGAA 
CCAGATGCTG TGTGTTCCTA TCAAAATTGC CATGGACATT CCAAAATCCA GATATATTTA 
ACTGATCCAG ATTTTAAGGG CTTTATACGT ATCAACTGCT GCCAATTGTG TAAAATAGAA 
TTTCACATTA GCTGCTGGAA GAAGTTAAAA ACAACTTTCA ATGATAAAAA TGACAAGGAT 
TTTCTTCAAG ACCCATGTCT TACCCCTGAC TGTGGAGGAA TCATTTCCAA GATTATTATC 
TTTGGTTCAA CAGGCCAAAT TAAATGTGAA TTTGAACATA AAGTTGTGAA AGAAAGGGAT 
CCACCGAGAC CTGTAGTGAA ACAGAAGTGT TCCAGCCTAG GGAAACTAAG AATTAAAGAA 
GATAAAAAAT CAAAGAGAAA GCTTCTAAAA AAAGAAGCAA AATCTTTAGC AAACGAAAGA 
ATGGAAGAGA AATCAAGTGA CAATAACTCA TCTAAACACA GTGTCCATAA AGGCTGTGTT 
CAAAGTTGCC AAGTTATTGG TGATAGAGTT CTGCATTGTA TAAAGCAAAA TGCTGAAAAA 
ATTAAATTTG GGGTACAAGA CACATCTAAA CTTCTGAGTG AATTGCTTTC TTGGTGGGTA 
ATAAGCAAGG AGGATTATAC TGAATGTTCT GCCAGCCATC TACCTAATGA AACCATGGAG 
CGCCTTCTCT GTCACTTGTT CCAGAACCAG AACAGAGTAA AGACAAGAAT TTTTTTACAT 
GTATTGAGTG AGATTGAAGT TGTGGTTGAC CCCAAACTAC ATGAGTGGAT TCAGAAACTC 
AACAATTTTG GCTTGGATGC CACAGAAGCT TTTTTTTCCC GTTATGGAGA TTCTATTAGA 
GAGCTCAAAT GCAACATCAT CACTGATCTT TGGAATGAGA AATATGGTCA TAAACTAGAC 
CTTGTGCTCC CCAATTCTGT AGATGGAGAA ATTCTTGAAT GCTTTCATTT GTCATCCTTG 
AAGGAAGCTC GCTGCCTAAT ATGGCTATTA GAAGAGAATA GGGAAAAATT TCCTACTCTA 
CATCATATTT TAGATGAATT TTTTGATATA ATGGATGGGC CCTGTACTAT ATTAAGGAAA 
CAAGAAAATG AAGATGCCTC AACTGTTATA AAGGTAAAAA ACAAAAACCG GAAAAAGAAG 
CAAAAAGGAT CAAAGCCTGT TTTCCCGCTG TCTGGAGTTG GAGGAATAAT ACAGGATGAT 
GACATTATTT TTACAGATGA AAATCCTCTA TCTTTTTTGA ATTCTTGGGA TCCGTTTGTT 
GTTCCTGCTA ACCTTCAAAA TGAGATAGAA GAATTTGAAG CACTCTATGA AAAAAAGATT 
TCTAATAGTC ATCATTATCA GAGACTATTG GATAATAATC CAGATCCAAA ATGTGAAACA 
CTTTATGACT ATTTCTCTCA AATACTGGAG GAACATGGTC CCCTGGAAAT TACAGATGAG 
TTATTGATTG GCGAATATGA ACACTTCCCT GAAGAAACTC GGAAAGTTGT GGAAGAAGCA 
GGGGGATTGA AATCTTTTCT CCTGAGATCT CTTCGTTTTG TTATGTTTAA CGACTTCATT 
GGACTGATGA AAGATGCAGT TGTCCTGAAG GAAAAGGAAG ATTACATGTT CAGTCCATAT 
TTTGACGAAC ACTCCTCACC AGTCAAACCA TTATTGAATC CAGCTGCAAA GGAATTCAAG 
CCATTCTCCC ATAACTGTAG TTCTAATGTG CCAATGTCTG AAACTATTAA AGAAAACAAT 
CAGACTGAAA AATATGTGGA CCCAGGACAT CCTTCAGCTT TTCCTTTTAA TTATAGTGCT 
GATGTTCGTT ATCACGGATT TCACATCAAT GAAGAATCAA TGTTTTGTTT AAATCTATTA 
CCATATTCAC CTGCCCATTT AAATTTCTAC ACACCTCCTG ATTATGAACT GGAAAAAATA 
TTACCAGTAG AGGCAGATAG GACAACTGTT GCTGACACTG CTAGACCAAA TAATGATGAT 
GAATATACTG ATCACAATGC TCCTCATTTT GATTCAGAAA AAGTAAAAGA GAGAGTCTTA 
GAAAGAGAGA ACATTTCTGG AAGGATGGTT TTTAGTGAAA CAGAGATATC AGCGAAATTA 
GACAACTGCA TTTATAGAGA TGATAATACT CAACTCCTGG GTAACAGTAA TGCTGGTCAT 
TATATTTCTA CTAATAAAAT GAAAATAGCG ACTAAAGATA TGGCATTGGT GAGAAAAAAT 
CCAGCTACAC GAATGATTGC TGTTCAGGTA AATCAGGACT TAACACATCA GGAGGTTAAT 
ACCATGCCTT TTAATCCATT TGAAACCCAA CATGGGGATA TTCTGCGAAT GGAAAAAGAA 
CACCAGGTAT TACAAGAGCA ACTGAGAGAA GCAAATGAAA AATATACACA GTTAAAACAC 
AGGAGTTCAG AAGAGATCAA AGATTTGGAA GAACAATTGA AAGAACTTTC TGAAAAGAAT 
AAGATCTCAA AGACTGAATT GGAATGGATG CACCAGGACC TGGAAAAAGA ACTGAAAAAA 
TGGCAACAGG AAAAAAAAGA AAATCAGGAA AAATTGAAAG GCCTAAAGAG TAAAATAAAG 
AAGTTTATAG AGAGCAATGA GAAGAATTTG AGGACTTTTT TAATGACTAA TGTTAGTGCT 
GTAAATCAGG ACTTAACACA TCAGGAGGTT AATACCATGC CTTTTAATCC ATTTGAAACC 
CAACATGGGG ATATTCTGCG AATGGAAAAA GAACACCAGG TATTACAAGA GCAACTGAGA 
GAAGCAAATG AAAAATATAC ACAGTTAAAA CACAGGAGTT CAGAAGAGAT CAAAGATTTG 
GAAGAACAAT TGAAAGAACT TTCTGAAAAG AATAAGATCT CAAAGACTGA ATTGGAATGG 
ATGCACCAGG ACCTGGAAAA AGAACTGAAA AAATGGCAAC AGGAAAAAAA AGAAAATCAG 
GAAAAATTGA AAGGCCTAAA GAGTAAAATA AAGAAGTTTA TAGAGAGCAA TGAGAACTGC 
TTAAGGACGG TCAAGGAAAA AGATCAGCAG TATGAATTGC ATTTGAATAA ATTCCTGGAG 
ATCAGTAATA AATTTGAAAA TGAGAAAGTG AAAATGGAGG AACATATAAA GAAGGGAAAA 
GATAATTACC AAGAGAGTAA TAAAAGATCT GTGGCAGCAG AGGTATCGGT CCTGAAAAAC 
TGGAAGGAAA AGGAACTGAG CAAGTTGTTC AGCAGAGTAT CAGAAGTAGA AAAATATCTT 
AAGCAACTAA AGTTAATGAA CAGTGATTCA TCTGAAGTGA AGCCTGAGAT AGCTTCTTGG 
GAGTTGTTTC TTTCAAAAAT TAAAAGAATA ATTGAGGAAA CAAAGGCTCA GTTTGAAGAT 
AGAATTCAGA AGATTCAAAG TGGCTCCCCA CTAGTCGGAC TTTCTAAAGT GCAAATTCCC 
GATCTTTCAA CTCCTTCCAG TAGTTTAAGA ACTCTAGTCA ACCTTCCTAT TAGCGATCCA 
GCCATTGTAA TGTATTCTCC ACCAATTCAT CCTAAATTAC TTCCTAAATA TCCACACCCT 
GAAGGTCAAA GTTCAGCGAA TCTTCCACGT CCTGTACAAA CTGGAAACCA AATGACGACA 
CGTAAAGTCT CAAGTCCGTA TCCTGCCAGT GATAGCTCCA AGGGGAAACT TGTGCAAAGA 
AATTACCTTG ATCCAGCTCT TCCCACTCTG CCTTCAGTGA CTACTAGGAA TCCTGATGTC 
CAAGTGAATC ACAGCCTAAT AGCTGACCGT CTGTCACCTA GTATTCAAAA ACAGCCTCCA 
CAGAAGCCAT TTGAAGCCAT TTTTAGAAAT CTGGCAATGA TTTTCCCCAA CTACAGCAGT 
AGTGAGTTTC TTTCTTTTAT AAAAGAAGTT CAGGCCAAAA ACAGGGGCAC GCTTTCAGGA 
TTGACCTCGG ATGAAATCAC CAGGAGAGTG ACAGATCTCA TCCTGGATGA GCCGAGTAAG 
AACAAGGCTG GAAAAGATAG GGCAGCGGTA TTTGTGGCAA ATGACCCTGG CCGAAGATCT 
TCGGAAGGAA GCGTGTTGGT CTCTGGCACC TCCAAGACCA GACCTGTTTC TAGCAAAAGC 
CCCAGCCACC CACCTCGAGG CCAGCCCTGG GTGATAGTTG GGGGGCCAAC CAAGACCAAG 
TGGCAGAAAT CGAAAGAGCC CACTACATCC GATGAAGATC CCTGTATCAT CTGTCATGAC 
GAACTAAATC AGGGCGTCCT TTGCAAACTG GAATGTGGGC ATCAGTTTCA CAAACAGGGC 
CGGGCCGGGC CGGGCCGGGC TGCCGGTGTT TCCGAGGAGG GAGGCCTCGG CCGGGACGGA 
AGGACGGAAG GACGGACCCC GGTTTCCCCG GCGCGGCTGC CAACGGAGCG AGGTGCCCTC 
GTTGGCCTTA AACCTTCCCC CGCCCGCGCC CGCGGCTTAG CCCGGCCCGG AGGGCCGTCC 
TGGGTTCGAA TCCCGCTTCT CAGCCGGGGG CCTCCGGGCC GGTCATTTGC CTTCTCCGTC 
TGTGTCCGTG CCCTCCTCTG CCAGGTGCGG GTTGGTCCTC CTTCCCACTA CGGCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TTC3 cDNA ORF clone, Ornithorhynchus anatinus(platypus)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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