TOP2A cDNA ORF clone, Ovis aries(sheep)

Gene
Clones
gRNAs

Summary

Gene Symbol	TOP2A
Entrez Gene ID	101108762
Full Name	DNA topoisomerase II alpha
Gene Type	protein-coding
Organism	Ovis aries(sheep)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012186069.2	XP_012041459.1	DNA topoisomerase 2-alpha isoform X2
XM_012141796.2	XP_011997186.1	DNA topoisomerase 2-alpha isoform X1
XM_012141797.2	XP_011997187.1	DNA topoisomerase 2-alpha isoform X2
XM_012186068.2	XP_012041458.1	DNA topoisomerase 2-alpha isoform X1
XM_012186068.3	XP_012041458.1	DNA topoisomerase 2-alpha

Pathways from BioSystems
Gene Ontology

The following TOP2A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOP2A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOv20838	XM_012141796.2 Latest version!	Ovis aries musimon topoisomerase (DNA) II alpha (TOP2A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OOv20839	XM_012141797.2 Latest version!	Ovis aries musimon topoisomerase (DNA) II alpha (TOP2A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OSm29655	XM_012186068.3 Latest version!	Ovis aries DNA topoisomerase II alpha (TOP2A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OSm29656	XM_012186069.2 Latest version!	Ovis aries topoisomerase (DNA) II alpha (TOP2A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
OSm29655	XM_012186068.2	Ovis aries topoisomerase (DNA) II alpha (TOP2A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TOP2A ( XM_012141796.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011997186.1 Ovis aries musimon topoisomerase (DNA) II alpha (TOP2A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOv20838
Clone ID Related Accession (Same CDS sequence)	XM_012141796.2
Accession Version	XM_012141796.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	DNA topoisomerase 2-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942481.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012141796.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGT CGCCACTACA GCCTGTAAAC GAAAATATGC AAGTCAGCAA AACAAAGAAA 
AATGAAGATG CTAAGAAAAG ATTGTCTGTT GAAAGAATCT ATCAAAAGAA AACACAACTG 
GAACATATCC TGCTCCGCCC AGACACCTAC ATTGGCTCTG TGGAATCAGT GACCCAGCAA 
ATGTGGGTTT ACGATGAAGA TACTGGCATT AACTATAGGG AAGTCACTTA TGTTCCTGGT 
TTGTACAAAA TCTTTGATGA GATTCTAGTC AATGCTGCAG ACAACAAACA AAGGGACCCA 
AAAATGTCTT GTATTAGAAT CACAATTGAC CCGGAAAACA ATTTAATTAG TATATGGAAT 
AATGGAAAAG GTATTCCTGT TGTCGAACAC AAAGTTGAGA AGATGTATGT CCCAGCTCTC 
ATCTTTGGAC AGCTCCTAAC TTCTAGCAAC TATGATGATG AAGAAAAGAA AGTAACAGGT 
GGTCGAAATG GCTATGGAGC CAAACTGTGT AACATATTCA GTACCAAATT TACTGTGGAA 
ACAGCAAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA CATGGGGAAA 
GCTGGTGAGA TGGAACTCAA GCCCTTTAGT GGAGAAGATT ATACATGTAT TACCTTCCAC 
CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTAGACAAAG ATATTGTTGC ACTGATGGTT 
AGAAGAGCAT ATGATATTGC TGGATCCACT AAAGATGTCA AGGTCTATCT CAATGGAAAT 
AGGCTTCCAG TGAAAGGATT CCGTAGCTAT GTAGATCTGT ATCTGAAGGA CAAGGTAGAT 
GAAACTGGCA ATCCATTGAA AATTATCCAT GAACAAGTAA ACCACAGGTG GGAAGTATGT 
TTAACAATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACTTCC 
AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT 
GTGAAGAAAA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTAAA AAATCACATG 
TGGATTTTTG TGAATGCCTT AATTGAAAAC CCAACCTTCG ACTCCCAGAC AAAAGAGAAC 
ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAGC TGAGTGAAAA ATTCATCAAA 
GCTGCAATTG GCTGTGGTAT TGTAGAAAGT ATACTAAATT GGGTGAAATT TAAGGCCCAA 
ATCCAGTTAA ACAAGAAGTG TTCTGCTGTA AAACATAACA GAATCAAAGG AATTCCCAAA 
CTTGATGATG CCAATGATGC AGGAGGCCGA AACTCTACTG AGTGTACACT TATCCTGACA 
GAGGGAGACT CAGCTAAAAC TTTAGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAG 
TATGGGGTTT TCCCTCTTAG AGGAAAAATA CTCAATGTAC GAGAAGCTTC TCATAAACAG 
ATAATGGAAA ATGCTGAAAT TAACAATATA ATCAAGATTG TGGGTCTTCA GTACAAGAAA 
AACTATGAAG ATGAAGATTC ATTGAAGACT CTACGTTATG GAAAGATAAT GATTATGACA 
GATCAGGACC AAGACGGCTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCATAAC 
TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCTAT TGTAAAGGTG 
TCTAAAAACA AGCAAGAAAT GGCATTCTAT AGCCTTCCTG AGTTTGAAGA ATGGAAGAGT 
TCTACACCAA ATCATAAAAA ATGGAAAGTC AAGTATTACA AAGGTTTGGG TACCAGCACA 
TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA ATTCAAATAT 
TCCGGTCCCG AAGATGATGC TGCAATCAGC CTGGCCTTTA GCAAAAAGCA GATAGATGAT 
CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGAC AACGAAAGTT ACTTGGCCTT 
CCTGATGATT ATTTGTATGG GCAAGCTACC ACATATCTGA CATACAATGA CTTCATAAAC 
AAGGAACTTA TCCTGTTCTC AAATTCTGAT AATGAAAGAT CTATCCCATC TATGGTGGAT 
GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACATGTT TCAAACGGAA TGACAAGCGA 
GAGGTGAAGG TTGCCCAGTT AGCTGGGTCA GTAGCTGAGA TGTCCTCTTA TCATCATGGT 
GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ACTTTGTGGG CAGCAATAAT 
CTGAACCTTT TGCAGCCCAT TGGTCAGTTT GGTACCAGGT TGCATGGTGG CAAGGACTCT 
GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTGTT ATTTCCAGCA 
AAAGATGACC ATACTTTAAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG 
TACATTCCTA TTATACCCAT GGTGTTGATA AATGGCGCTG AAGGAATTGG TACTGGGTGG 
TCCTGCAAAA TCCCCAACTT TGATGTTCGT GAAGTTGTGA ATAACATCAG ACGTTTGATG 
GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAG 
GAGCTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTGG CTATTCTTAA TTCCACAACC 
ATTGAAATCT CAGAGCTTCC CATCAGAACG TGGACCCAGA CGTATAAAGA ACAGGTTCTT 
GAGCCCATGT TGAATGGCAC TGAGAAGACA CCCCCTCTCA TAACAGATTA TAGGGAATAC 
CACACAGACA CCACTGTGAA GTTTGTTGTT AAGATGACTG AAGAAAAACT GGCAGAGGCA 
GAGAGAGTTG GACTACACAA AGTCTTTAAA CTCCAAACTA GTCTCACATG CAACTCCATG 
GTCCTCTTTG ACCATGTAGG CTGTTTAAAG AAATACGACA CAGCTTTGGA TATTCTAAGA 
GACTTCTTTG AACTCAGGCT TAAATATTAT GGATTAAGAA AAGAATGGCT TCTAGGAATG 
CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT 
GGCAAAATAA TCATTGAAAA TAAGCCCAAG AAAGAGTTAA TTAAAGTTCT GATTCAGAGA 
GGATATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA 
GAAGAAAATG AAGAGAGTGA CAATGAAAAG CAAAGTGAAA AGAGTGACTC TGCAACAGAT 
TCTGGACCCA CCTTCAACTA CCTTCTTGAC ATGCCTCTTT GGTATCTAAC CAAGGAAAAG 
AAAGATGAAC TGTGCAAACT AAGAAATGAA AAAGAGCAAG AGCTAGAAAC ATTAAAGAGA 
AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTGCTT TTGTTGAAGA GCTGGAGGTT 
GTTGAAGCAA AGGAAAAACA AGATGAACAA ATAGGACTTC CTGGGAAAGG TGGGAAAGCA 
AAGGGGAAAA AAACACAAAT GGTAGAAATT TTGCCTTCTC CAAGTGGAAA AAGAGTCATC 
CCCCGAGTGA CTGAAGAGAT GAAAGCAGAG GCAGAAAAGA AAATTAAAAA GAAAATTAAG 
AGTGAAAATA CTGAAGGAAG CCCTCAAGAG GAAGGAATGG AAGTAGAAAG CCTAAAACTG 
AGGCTGGAAA AGAAACAGAA AAGAGAGCCA GTTACCAAGA CAAAGAAACA AACTACATTG 
CCATTCAAAC CAATCAAGAA AGCAAAGAAA AGAAACCCCT GGTCTGATTC AGAATCAGAC 
ATGAGCAGTA ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAAAAGAGTC GCGGAGAGCA 
GCAGCAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAGATTTTTC AGATTTTGAT 
GAAGACACTC AAGATGATTT TGTCCCATCA AATGCTAGTC CACCTAAGAT CCAAACTTCC 
CCAAAACATA GTAACAAAGA ACTGAAGCCA CTGAAGAGTG CCTCATCAGT AACAGAGCTT 
GATGCTGATG ATGCTGAGGA TAATGTACCA CCTCCTCCAA GCTCTCCTGC TGCTGATTTC 
CCAGCTGTAA CTGAAACCAT AAACCCTGTT CCTAAAAAGA ATGTGACAGC AAAGAAGACA 
GCAGCAAAAA GTCAGTCTCC CACCTCCACT ACAGGCGCCA AGAAAAGGGC TGCACCAAAA 
AGAACCAAAA AGGACCCAGA CTTGGATTCT GATGTCTCTA AAAAGCCTAA TGCCCCTAAA 
ACCAAGACTC GCCGCAAAAG GAAGCCATCC TCCTCTGATG ATTCGGACTC TAATTTTGAG 
AAACTGATCT CCAAAGCAGT CACAAGCAAG AAACCCAAGG AGGAAAGCGA TGATTTCCAT 
CTGGACTTGG ACTCAGCTGT GGGTTCTCGG GCAAAATCTG GAAGAACAAA GAAACCTATA 
AAGTACCTTG AAGAATCAGA TGAAGATGAT TTCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011997186.1
CDS	144..4742
Translation	MEVSPLQPVNENMQVSKTKKNEDAKKRLSVERIYQKKTQLEHILLRPDTYIGSVESVTQQMWVYDEDTGINYREVTYVPGLYKIFDEILVNAADNKQRDPKMSCIRITIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDEEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGKAGEMELKPFSGEDYTCITFHPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVYLNGNRLPVKGFRSYVDLYLKDKVDETGNPLKIIHEQVNHRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVTKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQVKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQIQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEMAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTHFMEDRRQRKLLGLPDDYLYGQATTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPAKDDHTLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRRLMDGEEPLPMLPSYKNFKGTIEELAPNQYVISGEVAILNSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMTEEKLAEAERVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTALDILRDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNEKQSEKSDSATDSGPTFNYLLDMPLWYLTKEKKDELCKLRNEKEQELETLKRKSPSDLWKEDLAAFVEELEVVEAKEKQDEQIGLPGKGGKAKGKKTQMVEILPSPSGKRVIPRVTEEMKAEAEKKIKKKIKSENTEGSPQEEGMEVESLKLRLEKKQKREPVTKTKKQTTLPFKPIKKAKKRNPWSDSESDMSSNESNFDVPPREKESRRAAAKTKFTMDLDSDEDFSDFDEDTQDDFVPSNASPPKIQTSPKHSNKELKPLKSASSVTELDADDAEDNVPPPPSSPAADFPAVTETINPVPKKNVTAKKTAAKSQSPTSTTGAKKRAAPKRTKKDPDLDSDVSKKPNAPKTKTRRKRKPSSSDDSDSNFEKLISKAVTSKKPKEESDDFHLDLDSAVGSRAKSGRTKKPIKYLEESDEDDFF

Target ORF information:

RefSeq Version	XM_012141796.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon topoisomerase (DNA) II alpha (TOP2A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012141796.2

ORF Insert Sequence:

ATGGAGGTGT CGCCACTACA GCCTGTAAAC GAAAATATGC AAGTCAGCAA AACAAAGAAA 
AATGAAGATG CTAAGAAAAG ATTGTCTGTT GAAAGAATCT ATCAAAAGAA AACACAACTG 
GAACATATCC TGCTCCGCCC AGACACCTAC ATTGGCTCTG TGGAATCAGT GACCCAGCAA 
ATGTGGGTTT ACGATGAAGA TACTGGCATT AACTATAGGG AAGTCACTTA TGTTCCTGGT 
TTGTACAAAA TCTTTGATGA GATTCTAGTC AATGCTGCAG ACAACAAACA AAGGGACCCA 
AAAATGTCTT GTATTAGAAT CACAATTGAC CCGGAAAACA ATTTAATTAG TATATGGAAT 
AATGGAAAAG GTATTCCTGT TGTCGAACAC AAAGTTGAGA AGATGTATGT CCCAGCTCTC 
ATCTTTGGAC AGCTCCTAAC TTCTAGCAAC TATGATGATG AAGAAAAGAA AGTAACAGGT 
GGTCGAAATG GCTATGGAGC CAAACTGTGT AACATATTCA GTACCAAATT TACTGTGGAA 
ACAGCAAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA CATGGGGAAA 
GCTGGTGAGA TGGAACTCAA GCCCTTTAGT GGAGAAGATT ATACATGTAT TACCTTCCAC 
CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTAGACAAAG ATATTGTTGC ACTGATGGTT 
AGAAGAGCAT ATGATATTGC TGGATCCACT AAAGATGTCA AGGTCTATCT CAATGGAAAT 
AGGCTTCCAG TGAAAGGATT CCGTAGCTAT GTAGATCTGT ATCTGAAGGA CAAGGTAGAT 
GAAACTGGCA ATCCATTGAA AATTATCCAT GAACAAGTAA ACCACAGGTG GGAAGTATGT 
TTAACAATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACTTCC 
AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT 
GTGAAGAAAA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTAAA AAATCACATG 
TGGATTTTTG TGAATGCCTT AATTGAAAAC CCAACCTTCG ACTCCCAGAC AAAAGAGAAC 
ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAGC TGAGTGAAAA ATTCATCAAA 
GCTGCAATTG GCTGTGGTAT TGTAGAAAGT ATACTAAATT GGGTGAAATT TAAGGCCCAA 
ATCCAGTTAA ACAAGAAGTG TTCTGCTGTA AAACATAACA GAATCAAAGG AATTCCCAAA 
CTTGATGATG CCAATGATGC AGGAGGCCGA AACTCTACTG AGTGTACACT TATCCTGACA 
GAGGGAGACT CAGCTAAAAC TTTAGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAG 
TATGGGGTTT TCCCTCTTAG AGGAAAAATA CTCAATGTAC GAGAAGCTTC TCATAAACAG 
ATAATGGAAA ATGCTGAAAT TAACAATATA ATCAAGATTG TGGGTCTTCA GTACAAGAAA 
AACTATGAAG ATGAAGATTC ATTGAAGACT CTACGTTATG GAAAGATAAT GATTATGACA 
GATCAGGACC AAGACGGCTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCATAAC 
TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCTAT TGTAAAGGTG 
TCTAAAAACA AGCAAGAAAT GGCATTCTAT AGCCTTCCTG AGTTTGAAGA ATGGAAGAGT 
TCTACACCAA ATCATAAAAA ATGGAAAGTC AAGTATTACA AAGGTTTGGG TACCAGCACA 
TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA ATTCAAATAT 
TCCGGTCCCG AAGATGATGC TGCAATCAGC CTGGCCTTTA GCAAAAAGCA GATAGATGAT 
CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGAC AACGAAAGTT ACTTGGCCTT 
CCTGATGATT ATTTGTATGG GCAAGCTACC ACATATCTGA CATACAATGA CTTCATAAAC 
AAGGAACTTA TCCTGTTCTC AAATTCTGAT AATGAAAGAT CTATCCCATC TATGGTGGAT 
GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACATGTT TCAAACGGAA TGACAAGCGA 
GAGGTGAAGG TTGCCCAGTT AGCTGGGTCA GTAGCTGAGA TGTCCTCTTA TCATCATGGT 
GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ACTTTGTGGG CAGCAATAAT 
CTGAACCTTT TGCAGCCCAT TGGTCAGTTT GGTACCAGGT TGCATGGTGG CAAGGACTCT 
GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTGTT ATTTCCAGCA 
AAAGATGACC ATACTTTAAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG 
TACATTCCTA TTATACCCAT GGTGTTGATA AATGGCGCTG AAGGAATTGG TACTGGGTGG 
TCCTGCAAAA TCCCCAACTT TGATGTTCGT GAAGTTGTGA ATAACATCAG ACGTTTGATG 
GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAG 
GAGCTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTGG CTATTCTTAA TTCCACAACC 
ATTGAAATCT CAGAGCTTCC CATCAGAACG TGGACCCAGA CGTATAAAGA ACAGGTTCTT 
GAGCCCATGT TGAATGGCAC TGAGAAGACA CCCCCTCTCA TAACAGATTA TAGGGAATAC 
CACACAGACA CCACTGTGAA GTTTGTTGTT AAGATGACTG AAGAAAAACT GGCAGAGGCA 
GAGAGAGTTG GACTACACAA AGTCTTTAAA CTCCAAACTA GTCTCACATG CAACTCCATG 
GTCCTCTTTG ACCATGTAGG CTGTTTAAAG AAATACGACA CAGCTTTGGA TATTCTAAGA 
GACTTCTTTG AACTCAGGCT TAAATATTAT GGATTAAGAA AAGAATGGCT TCTAGGAATG 
CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT 
GGCAAAATAA TCATTGAAAA TAAGCCCAAG AAAGAGTTAA TTAAAGTTCT GATTCAGAGA 
GGATATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA 
GAAGAAAATG AAGAGAGTGA CAATGAAAAG CAAAGTGAAA AGAGTGACTC TGCAACAGAT 
TCTGGACCCA CCTTCAACTA CCTTCTTGAC ATGCCTCTTT GGTATCTAAC CAAGGAAAAG 
AAAGATGAAC TGTGCAAACT AAGAAATGAA AAAGAGCAAG AGCTAGAAAC ATTAAAGAGA 
AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTGCTT TTGTTGAAGA GCTGGAGGTT 
GTTGAAGCAA AGGAAAAACA AGATGAACAA ATAGGACTTC CTGGGAAAGG TGGGAAAGCA 
AAGGGGAAAA AAACACAAAT GGTAGAAATT TTGCCTTCTC CAAGTGGAAA AAGAGTCATC 
CCCCGAGTGA CTGAAGAGAT GAAAGCAGAG GCAGAAAAGA AAATTAAAAA GAAAATTAAG 
AGTGAAAATA CTGAAGGAAG CCCTCAAGAG GAAGGAATGG AAGTAGAAAG CCTAAAACTG 
AGGCTGGAAA AGAAACAGAA AAGAGAGCCA GTTACCAAGA CAAAGAAACA AACTACATTG 
CCATTCAAAC CAATCAAGAA AGCAAAGAAA AGAAACCCCT GGTCTGATTC AGAATCAGAC 
ATGAGCAGTA ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAAAAGAGTC GCGGAGAGCA 
GCAGCAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAGATTTTTC AGATTTTGAT 
GAAGACACTC AAGATGATTT TGTCCCATCA AATGCTAGTC CACCTAAGAT CCAAACTTCC 
CCAAAACATA GTAACAAAGA ACTGAAGCCA CTGAAGAGTG CCTCATCAGT AACAGAGCTT 
GATGCTGATG ATGCTGAGGA TAATGTACCA CCTCCTCCAA GCTCTCCTGC TGCTGATTTC 
CCAGCTGTAA CTGAAACCAT AAACCCTGTT CCTAAAAAGA ATGTGACAGC AAAGAAGACA 
GCAGCAAAAA GTCAGTCTCC CACCTCCACT ACAGGCGCCA AGAAAAGGGC TGCACCAAAA 
AGAACCAAAA AGGACCCAGA CTTGGATTCT GATGTCTCTA AAAAGCCTAA TGCCCCTAAA 
ACCAAGACTC GCCGCAAAAG GAAGCCATCC TCCTCTGATG ATTCGGACTC TAATTTTGAG 
AAACTGATCT CCAAAGCAGT CACAAGCAAG AAACCCAAGG AGGAAAGCGA TGATTTCCAT 
CTGGACTTGG ACTCAGCTGT GGGTTCTCGG GCAAAATCTG GAAGAACAAA GAAACCTATA 
AAGTACCTTG AAGAATCAGA TGAAGATGAT TTCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP2A ( XM_012141797.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011997187.1 Ovis aries musimon topoisomerase (DNA) II alpha (TOP2A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOv20839
Clone ID Related Accession (Same CDS sequence)	XM_012141797.2
Accession Version	XM_012141797.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	DNA topoisomerase 2-alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942481.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012141797.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGT CGCCACTACA GCCTGTAAAC GAAAATATGC AAGTCAGCAA AACAAAGAAA 
AATGAAGATG CTAAGAAAAG ATTGTCTGTT GAAAGAATCT ATCAAAAGAA AACACAACTG 
GAACATATCC TGCTCCGCCC AGACACCTAC ATTGGCTCTG TGGAATCAGT GACCCAGCAA 
ATGTGGGTTT ACGATGAAGA TACTGGCATT AACTATAGGG AAGTCACTTA TGTTCCTGGT 
TTGTACAAAA TCTTTGATGA GATTCTAGTC AATGCTGCAG ACAACAAACA AAGGGACCCA 
AAAATGTCTT GTATTAGAAT CACAATTGAC CCGGAAAACA ATTTAATTAG TATATGGAAT 
AATGGAAAAG GTATTCCTGT TGTCGAACAC AAAGTTGAGA AGATGTATGT CCCAGCTCTC 
ATCTTTGGAC AGCTCCTAAC TTCTAGCAAC TATGATGATG AAGAAAAGAA AGTAACAGGT 
GGTCGAAATG GCTATGGAGC CAAACTGTGT AACATATTCA GTACCAAATT TACTGTGGAA 
ACAGCAAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA CATGGGGAAA 
GCTGGTGAGA TGGAACTCAA GCCCTTTAGT GGAGAAGATT ATACATGTAT TACCTTCCAC 
CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTAGACAAAG ATATTGTTGC ACTGATGGTT 
AGAAGAGCAT ATGATATTGC TGGATCCACT AAAGATGTCA AGGTCTATCT CAATGGAAAT 
AGGCTTCCAG TGAAAGGATT CCGTAGCTAT GTAGATCTGT ATCTGAAGGA CAAGGTAGAT 
GAAACTGGCA ATCCATTGAA AATTATCCAT GAACAAGTAA ACCACAGGTG GGAAGTATGT 
TTAACAATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACTTCC 
AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT 
GTGAAGAAAA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTAAA AAATCACATG 
TGGATTTTTG TGAATGCCTT AATTGAAAAC CCAACCTTCG ACTCCCAGAC AAAAGAGAAC 
ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAGC TGAGTGAAAA ATTCATCAAA 
GCTGCAATTG GCTGTGGTAT TGTAGAAAGT ATACTAAATT GGGTGAAATT TAAGGCCCAA 
ATCCAGTTAA ACAAGAAGTG TTCTGCTGTA AAACATAACA GAATCAAAGG AATTCCCAAA 
CTTGATGATG CCAATGATGC AGGAGGCCGA AACTCTACTG AGTGTACACT TATCCTGACA 
GAGGGAGACT CAGCTAAAAC TTTAGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAG 
TATGGGGTTT TCCCTCTTAG AGGAAAAATA CTCAATGTAC GAGAAGCTTC TCATAAACAG 
ATAATGGAAA ATGCTGAAAT TAACAATATA ATCAAGATTG TGGGTCTTCA GTACAAGAAA 
AACTATGAAG ATGAAGATTC ATTGAAGACT CTACGTTATG GAAAGATAAT GATTATGACA 
GATCAGGACC AAGACGGCTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCATAAC 
TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCTAT TGTAAAGGTG 
TCTAAAAACA AGCAAGAAAT GGCATTCTAT AGCCTTCCTG AGTTTGAAGA ATGGAAGAGT 
TCTACACCAA ATCATAAAAA ATGGAAAGTC AAGTATTACA AAGGTTTGGG TACCAGCACA 
TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA ATTCAAATAT 
TCCGGTCCCG AAGATGATGC TGCAATCAGC CTGGCCTTTA GCAAAAAGCA GATAGATGAT 
CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGAC AACGAAAGTT ACTTGGCCTT 
CCTGATGATT ATTTGTATGG GCAAGCTACC ACATATCTGA CATACAATGA CTTCATAAAC 
AAGGAACTTA TCCTGTTCTC AAATTCTGAT AATGAAAGAT CTATCCCATC TATGGTGGAT 
GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACATGTT TCAAACGGAA TGACAAGCGA 
GAGGTGAAGG TTGCCCAGTT AGCTGGGTCA GTAGCTGAGA TGTCCTCTTA TCATCATGGT 
GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ACTTTGTGGG CAGCAATAAT 
CTGAACCTTT TGCAGCCCAT TGGTCAGTTT GGTACCAGGT TGCATGGTGG CAAGGACTCT 
GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTGTT ATTTCCAGCA 
AAAGATGACC ATACTTTAAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG 
TACATTCCTA TTATACCCAT GGTGTTGATA AATGGCGCTG AAGGAATTGG TACTGGGTGG 
TCCTGCAAAA TCCCCAACTT TGATGTTCGT GAAGTTGTGA ATAACATCAG ACGTTTGATG 
GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAG 
GAGCTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTGG CTATTCTTAA TTCCACAACC 
ATTGAAATCT CAGAGCTTCC CATCAGAACG TGGACCCAGA CGTATAAAGA ACAGGTTCTT 
GAGCCCATGT TGAATGGCAC TGAGAAGACA CCCCCTCTCA TAACAGATTA TAGGGAATAC 
CACACAGACA CCACTGTGAA GTTTGTTGTT AAGATGACTG AAGAAAAACT GGCAGAGGCA 
GAGAGAGTTG GACTACACAA AGTCTTTAAA CTCCAAACTA GTCTCACATG CAACTCCATG 
GTCCTCTTTG ACCATGTAGG CTGTTTAAAG AAATACGACA CAGCTTTGGA TATTCTAAGA 
GACTTCTTTG AACTCAGGCT TAAATATTAT GGATTAAGAA AAGAATGGCT TCTAGGAATG 
CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT 
GGCAAAATAA TCATTGAAAA TAAGCCCAAG AAAGAGTTAA TTAAAGTTCT GATTCAGAGA 
GGATATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA 
GAAGAAAATG AAGAGAGTGA CAATGAAAAG CAAAGTGAAA AGAGTGACTC TGCAACAGAT 
TCTGGACCCA CCTTCAACTA CCTTCTTGAC ATGCCTCTTT GGTATCTAAC CAAGGAAAAG 
AAAGATGAAC TGTGCAAACT AAGAAATGAA AAAGAGCAAG AGCTAGAAAC ATTAAAGAGA 
AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTGCTT TTGTTGAAGA GCTGGAGGTT 
GTTGAAGCAA AGGAAAAACA AGATGAACAA ATAGGACTTC CTGGGAAAGG TGGGAAAGCA 
AAGGGGAAAA AAACACAAAT GGTAGAAATT TTGCCTTCTC CAAGTGGAAA AAGAGTCATC 
CCCCGAGTGA CTGAAGAGAT GAAAGCAGAG GCAGAAAAGA AAATTAAAAA GAAAATTAAG 
AGTGAAAATA CTGAAGGAAG CCCTCAAGAG GAAGGAATGG AAGTAGAAAG CCTAAAACTG 
AGGCTGGAAA AGAAACAGAA AAGAGAGCCA GTTACCAAGA CAAAGAAACA AACTACATTG 
CCATTCAAAC CAATCAAGAA AGCAAAGAAA AGAAACCCCT GGTCTGATTC AGAATCAGAC 
ATGAGCAGTA ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAAAAGAGTC GCGGAGAGCA 
GCAGCAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAGATTTTTC AGATTTTGAT 
GAAGACACTC AAGATGATTT TGTCCCATCA AATGCTAGTC CACCTAAGAT CCAAACTTCC 
CCAAAACATA GTAACAAAGA ACTGAAGCCA CTGAAGAGTG CCTCATCAGT AACAGAGCTT 
GATGCTGATG ATGCTGAGGA TAATGTACCA CCTCCTCCAA GCTCTCCTGC TGCTGATTTC 
CCAGCTGTAA CTGAAACCAT AAACCCTGTT CCTAAAAAGA ATGTGACAGC AAAGAAGACA 
GCAGCAAAAA GCGCCAAGAA AAGGGCTGCA CCAAAAAGAA CCAAAAAGGA CCCAGACTTG 
GATTCTGATG TCTCTAAAAA GCCTAATGCC CCTAAAACCA AGACTCGCCG CAAAAGGAAG 
CCATCCTCCT CTGATGATTC GGACTCTAAT TTTGAGAAAC TGATCTCCAA AGCAGTCACA 
AGCAAGAAAC CCAAGGAGGA AAGCGATGAT TTCCATCTGG ACTTGGACTC AGCTGTGGGT 
TCTCGGGCAA AATCTGGAAG AACAAAGAAA CCTATAAAGT ACCTTGAAGA ATCAGATGAA 
GATGATTTCT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011997187.1
CDS	144..4718
Translation	MEVSPLQPVNENMQVSKTKKNEDAKKRLSVERIYQKKTQLEHILLRPDTYIGSVESVTQQMWVYDEDTGINYREVTYVPGLYKIFDEILVNAADNKQRDPKMSCIRITIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDEEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGKAGEMELKPFSGEDYTCITFHPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVYLNGNRLPVKGFRSYVDLYLKDKVDETGNPLKIIHEQVNHRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVTKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQVKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQIQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEMAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTHFMEDRRQRKLLGLPDDYLYGQATTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPAKDDHTLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRRLMDGEEPLPMLPSYKNFKGTIEELAPNQYVISGEVAILNSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMTEEKLAEAERVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTALDILRDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNEKQSEKSDSATDSGPTFNYLLDMPLWYLTKEKKDELCKLRNEKEQELETLKRKSPSDLWKEDLAAFVEELEVVEAKEKQDEQIGLPGKGGKAKGKKTQMVEILPSPSGKRVIPRVTEEMKAEAEKKIKKKIKSENTEGSPQEEGMEVESLKLRLEKKQKREPVTKTKKQTTLPFKPIKKAKKRNPWSDSESDMSSNESNFDVPPREKESRRAAAKTKFTMDLDSDEDFSDFDEDTQDDFVPSNASPPKIQTSPKHSNKELKPLKSASSVTELDADDAEDNVPPPPSSPAADFPAVTETINPVPKKNVTAKKTAAKSAKKRAAPKRTKKDPDLDSDVSKKPNAPKTKTRRKRKPSSSDDSDSNFEKLISKAVTSKKPKEESDDFHLDLDSAVGSRAKSGRTKKPIKYLEESDEDDFF

Target ORF information:

RefSeq Version	XM_012141797.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon topoisomerase (DNA) II alpha (TOP2A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012141797.2

ORF Insert Sequence:

ATGGAGGTGT CGCCACTACA GCCTGTAAAC GAAAATATGC AAGTCAGCAA AACAAAGAAA 
AATGAAGATG CTAAGAAAAG ATTGTCTGTT GAAAGAATCT ATCAAAAGAA AACACAACTG 
GAACATATCC TGCTCCGCCC AGACACCTAC ATTGGCTCTG TGGAATCAGT GACCCAGCAA 
ATGTGGGTTT ACGATGAAGA TACTGGCATT AACTATAGGG AAGTCACTTA TGTTCCTGGT 
TTGTACAAAA TCTTTGATGA GATTCTAGTC AATGCTGCAG ACAACAAACA AAGGGACCCA 
AAAATGTCTT GTATTAGAAT CACAATTGAC CCGGAAAACA ATTTAATTAG TATATGGAAT 
AATGGAAAAG GTATTCCTGT TGTCGAACAC AAAGTTGAGA AGATGTATGT CCCAGCTCTC 
ATCTTTGGAC AGCTCCTAAC TTCTAGCAAC TATGATGATG AAGAAAAGAA AGTAACAGGT 
GGTCGAAATG GCTATGGAGC CAAACTGTGT AACATATTCA GTACCAAATT TACTGTGGAA 
ACAGCAAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA CATGGGGAAA 
GCTGGTGAGA TGGAACTCAA GCCCTTTAGT GGAGAAGATT ATACATGTAT TACCTTCCAC 
CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTAGACAAAG ATATTGTTGC ACTGATGGTT 
AGAAGAGCAT ATGATATTGC TGGATCCACT AAAGATGTCA AGGTCTATCT CAATGGAAAT 
AGGCTTCCAG TGAAAGGATT CCGTAGCTAT GTAGATCTGT ATCTGAAGGA CAAGGTAGAT 
GAAACTGGCA ATCCATTGAA AATTATCCAT GAACAAGTAA ACCACAGGTG GGAAGTATGT 
TTAACAATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACTTCC 
AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT 
GTGAAGAAAA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTAAA AAATCACATG 
TGGATTTTTG TGAATGCCTT AATTGAAAAC CCAACCTTCG ACTCCCAGAC AAAAGAGAAC 
ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAGC TGAGTGAAAA ATTCATCAAA 
GCTGCAATTG GCTGTGGTAT TGTAGAAAGT ATACTAAATT GGGTGAAATT TAAGGCCCAA 
ATCCAGTTAA ACAAGAAGTG TTCTGCTGTA AAACATAACA GAATCAAAGG AATTCCCAAA 
CTTGATGATG CCAATGATGC AGGAGGCCGA AACTCTACTG AGTGTACACT TATCCTGACA 
GAGGGAGACT CAGCTAAAAC TTTAGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAG 
TATGGGGTTT TCCCTCTTAG AGGAAAAATA CTCAATGTAC GAGAAGCTTC TCATAAACAG 
ATAATGGAAA ATGCTGAAAT TAACAATATA ATCAAGATTG TGGGTCTTCA GTACAAGAAA 
AACTATGAAG ATGAAGATTC ATTGAAGACT CTACGTTATG GAAAGATAAT GATTATGACA 
GATCAGGACC AAGACGGCTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCATAAC 
TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCTAT TGTAAAGGTG 
TCTAAAAACA AGCAAGAAAT GGCATTCTAT AGCCTTCCTG AGTTTGAAGA ATGGAAGAGT 
TCTACACCAA ATCATAAAAA ATGGAAAGTC AAGTATTACA AAGGTTTGGG TACCAGCACA 
TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA ATTCAAATAT 
TCCGGTCCCG AAGATGATGC TGCAATCAGC CTGGCCTTTA GCAAAAAGCA GATAGATGAT 
CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGAC AACGAAAGTT ACTTGGCCTT 
CCTGATGATT ATTTGTATGG GCAAGCTACC ACATATCTGA CATACAATGA CTTCATAAAC 
AAGGAACTTA TCCTGTTCTC AAATTCTGAT AATGAAAGAT CTATCCCATC TATGGTGGAT 
GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACATGTT TCAAACGGAA TGACAAGCGA 
GAGGTGAAGG TTGCCCAGTT AGCTGGGTCA GTAGCTGAGA TGTCCTCTTA TCATCATGGT 
GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ACTTTGTGGG CAGCAATAAT 
CTGAACCTTT TGCAGCCCAT TGGTCAGTTT GGTACCAGGT TGCATGGTGG CAAGGACTCT 
GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTGTT ATTTCCAGCA 
AAAGATGACC ATACTTTAAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG 
TACATTCCTA TTATACCCAT GGTGTTGATA AATGGCGCTG AAGGAATTGG TACTGGGTGG 
TCCTGCAAAA TCCCCAACTT TGATGTTCGT GAAGTTGTGA ATAACATCAG ACGTTTGATG 
GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAG 
GAGCTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTGG CTATTCTTAA TTCCACAACC 
ATTGAAATCT CAGAGCTTCC CATCAGAACG TGGACCCAGA CGTATAAAGA ACAGGTTCTT 
GAGCCCATGT TGAATGGCAC TGAGAAGACA CCCCCTCTCA TAACAGATTA TAGGGAATAC 
CACACAGACA CCACTGTGAA GTTTGTTGTT AAGATGACTG AAGAAAAACT GGCAGAGGCA 
GAGAGAGTTG GACTACACAA AGTCTTTAAA CTCCAAACTA GTCTCACATG CAACTCCATG 
GTCCTCTTTG ACCATGTAGG CTGTTTAAAG AAATACGACA CAGCTTTGGA TATTCTAAGA 
GACTTCTTTG AACTCAGGCT TAAATATTAT GGATTAAGAA AAGAATGGCT TCTAGGAATG 
CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT 
GGCAAAATAA TCATTGAAAA TAAGCCCAAG AAAGAGTTAA TTAAAGTTCT GATTCAGAGA 
GGATATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA 
GAAGAAAATG AAGAGAGTGA CAATGAAAAG CAAAGTGAAA AGAGTGACTC TGCAACAGAT 
TCTGGACCCA CCTTCAACTA CCTTCTTGAC ATGCCTCTTT GGTATCTAAC CAAGGAAAAG 
AAAGATGAAC TGTGCAAACT AAGAAATGAA AAAGAGCAAG AGCTAGAAAC ATTAAAGAGA 
AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTGCTT TTGTTGAAGA GCTGGAGGTT 
GTTGAAGCAA AGGAAAAACA AGATGAACAA ATAGGACTTC CTGGGAAAGG TGGGAAAGCA 
AAGGGGAAAA AAACACAAAT GGTAGAAATT TTGCCTTCTC CAAGTGGAAA AAGAGTCATC 
CCCCGAGTGA CTGAAGAGAT GAAAGCAGAG GCAGAAAAGA AAATTAAAAA GAAAATTAAG 
AGTGAAAATA CTGAAGGAAG CCCTCAAGAG GAAGGAATGG AAGTAGAAAG CCTAAAACTG 
AGGCTGGAAA AGAAACAGAA AAGAGAGCCA GTTACCAAGA CAAAGAAACA AACTACATTG 
CCATTCAAAC CAATCAAGAA AGCAAAGAAA AGAAACCCCT GGTCTGATTC AGAATCAGAC 
ATGAGCAGTA ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAAAAGAGTC GCGGAGAGCA 
GCAGCAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAGATTTTTC AGATTTTGAT 
GAAGACACTC AAGATGATTT TGTCCCATCA AATGCTAGTC CACCTAAGAT CCAAACTTCC 
CCAAAACATA GTAACAAAGA ACTGAAGCCA CTGAAGAGTG CCTCATCAGT AACAGAGCTT 
GATGCTGATG ATGCTGAGGA TAATGTACCA CCTCCTCCAA GCTCTCCTGC TGCTGATTTC 
CCAGCTGTAA CTGAAACCAT AAACCCTGTT CCTAAAAAGA ATGTGACAGC AAAGAAGACA 
GCAGCAAAAA GCGCCAAGAA AAGGGCTGCA CCAAAAAGAA CCAAAAAGGA CCCAGACTTG 
GATTCTGATG TCTCTAAAAA GCCTAATGCC CCTAAAACCA AGACTCGCCG CAAAAGGAAG 
CCATCCTCCT CTGATGATTC GGACTCTAAT TTTGAGAAAC TGATCTCCAA AGCAGTCACA 
AGCAAGAAAC CCAAGGAGGA AAGCGATGAT TTCCATCTGG ACTTGGACTC AGCTGTGGGT 
TCTCGGGCAA AATCTGGAAG AACAAAGAAA CCTATAAAGT ACCTTGAAGA ATCAGATGAA 
GATGATTTCT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP2A ( XM_012186068.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012041458.1 Ovis aries topoisomerase (DNA) II alpha (TOP2A), transcript variant X1, mRNA.

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CloneID	OSm29655
Clone ID Related Accession (Same CDS sequence)	XM_012186068.2 , XM_012186068.3
Accession Version	XM_012186068.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	DNA topoisomerase 2-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639020.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012186068.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGT CGCCACTACA GCCTGTAAAC GAAAATATGC AAGTCAGCAA AACAAAGAAA 
AATGAAGATG CTAAGAAAAG ATTGTCTGTT GAAAGAATCT ATCAAAAGAA AACACAACTG 
GAACATATCC TGCTCCGCCC AGACACCTAC ATTGGCTCTG TGGAATCAGT GACCCAGCAA 
ATGTGGGTTT ACGATGAAGA TACTGGCATT AACTATAGGG AAGTCACTTA TGTTCCTGGT 
TTGTACAAAA TCTTTGATGA GATTCTAGTC AATGCTGCAG ACAACAAACA AAGGGACCCA 
AAAATGTCTT GTATTAGAAT CACAATTGAC CCGGAAAACA ATTTAATTAG TATATGGAAT 
AATGGAAAAG GTATTCCTGT TGTCGAACAC AAAGTTGAGA AGATGTATGT CCCAGCTCTC 
ATCTTTGGAC AGCTCCTAAC TTCTAGCAAC TATGATGATG AAGAAAAGAA AGTAACAGGT 
GGTCGAAATG GCTATGGAGC CAAACTGTGT AACATATTCA GTACCAAATT TACTGTGGAA 
ACAGCAAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA CATGGGGAAA 
GCTGGTGAGA TGGAACTCAA GCCCTTTAGT GGAGAAGATT ATACATGTAT TACCTTCCAC 
CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTAGACAAAG ATATTGTTGC ACTGATGGTT 
AGAAGAGCAT ATGATATTGC TGGATCCACT AAAGATGTCA AGGTCTATCT CAATGGAAAT 
AGGCTTCCAG TGAAAGGATT CCGTAGCTAT GTAGATCTGT ATCTGAAGGA CAAGGTAGAT 
GAAACTGGCA ATCCATTGAA AATTATCCAT GAACAAGTAA ACCACAGGTG GGAAGTATGT 
TTAACAATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACTTCC 
AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT 
GTGAAGAAAA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTAAA AAATCACATG 
TGGATTTTTG TGAATGCCTT AATTGAAAAC CCAACCTTCG ACTCCCAGAC AAAAGAGAAC 
ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAGC TGAGTGAAAA ATTCATCAAA 
GCTGCAATTG GCTGTGGTAT TGTAGAAAGT ATACTAAATT GGGTGAAATT TAAGGCCCAA 
ATCCAGTTAA ACAAGAAGTG TTCTGCTGTA AAACATAACA GAATCAAAGG AATTCCCAAA 
CTTGATGATG CCAATGATGC AGGAGGCCGA AACTCTACTG AGTGTACACT TATCCTGACA 
GAGGGAGACT CAGCTAAAAC TTTAGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAG 
TATGGGGTTT TCCCTCTTAG AGGAAAAATA CTCAATGTAC GAGAAGCTTC TCATAAACAG 
ATAATGGAAA ATGCTGAAAT TAACAATATA ATCAAGATTG TGGGTCTTCA GTACAAGAAA 
AACTATGAAG ATGAAGATTC ATTGAAGACT CTACGTTATG GAAAGATAAT GATTATGACA 
GATCAGGACC AAGACGGCTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCATAAC 
TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCTAT TGTAAAGGTG 
TCTAAAAACA AGCAAGAAAT GGCATTCTAT AGCCTTCCTG AGTTTGAAGA ATGGAAGAGT 
TCTACACCAA ATCATAAAAA ATGGAAAGTC AAGTATTACA AAGGTTTGGG TACCAGCACA 
TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA ATTCAAATAT 
TCCGGTCCCG AAGATGATGC TGCAATCAGC CTGGCCTTTA GCAAAAAGCA GATAGATGAT 
CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGAC AACGAAAGTT ACTTGGCCTT 
CCTGATGATT ATTTGTATGG GCAAGCTACC ACATATCTGA CATACAATGA CTTCATAAAC 
AAGGAACTTA TCCTGTTCTC AAATTCTGAT AATGAAAGAT CTATCCCATC TATGGTGGAT 
GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACATGTT TCAAACGGAA TGACAAGCGA 
GAGGTGAAGG TTGCCCAGTT AGCTGGGTCA GTAGCTGAGA TGTCCTCTTA TCATCATGGT 
GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ACTTTGTGGG CAGCAATAAT 
CTGAACCTTT TGCAGCCCAT TGGTCAGTTT GGTACCAGGT TGCATGGTGG CAAGGACTCT 
GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTGTT ATTTCCAGCA 
AAAGATGACC ATACTTTAAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG 
TACATTCCTA TTATACCCAT GGTGTTGATA AATGGCGCTG AAGGAATTGG TACTGGGTGG 
TCCTGCAAAA TCCCCAACTT TGATGTTCGT GAAGTTGTGA ATAACATCAG ACGTTTGATG 
GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAG 
GAGCTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTGG CTATTCTTAA TTCCACAACC 
ATTGAAATCT CAGAGCTTCC CATCAGAACG TGGACCCAGA CGTATAAAGA ACAGGTTCTT 
GAGCCCATGT TGAATGGCAC TGAGAAGACA CCCCCTCTCA TAACAGATTA TAGGGAATAC 
CACACAGACA CCACTGTGAA GTTTGTTGTT AAGATGACTG AAGAAAAACT GGCAGAGGCA 
GAGAGAGTTG GACTACACAA AGTCTTTAAA CTCCAAACTA GTCTCACATG CAACTCCATG 
GTCCTCTTTG ACCATGTAGG CTGTTTAAAG AAATACGACA CAGCTTTGGA TATTCTAAGA 
GACTTCTTTG AACTCAGGCT TAAATATTAT GGATTAAGAA AAGAATGGCT TCTAGGAATG 
CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT 
GGCAAAATAA TCATTGAAAA TAAGCCCAAG AAAGAGTTAA TTAAAGTTCT GATTCAGAGA 
GGATATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA 
GAAGAAAATG AAGAGAGTGA CAATGAAAAG CAAAGTGAAA AGAGTGACTC TGCAACAGAT 
TCTGGACCCA CCTTCAACTA CCTTCTTGAC ATGCCTCTTT GGTATCTAAC CAAGGAAAAG 
AAAGATGAAC TGTGCAAACT AAGAAATGAA AAAGAGCAAG AGCTAGAAAC ATTAAAGAGA 
AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTGCTT TTGTTGAAGA GCTGGAGGTT 
GTTGAAGCAA AGGAAAAACA AGATGAACAA ATAGGACTTC CTGGGAAAGG TGGGAAAGCA 
AAGGGGAAAA AAACACAAAT GGTAGAAATT TTGCCTTCTC CAAGTGGAAA AAGAGTCATC 
CCCCGAGTGA CTGAAGAGAT GAAAGCAGAG GCAGAAAAGA AAATTAAAAA GAAAATTAAG 
AGTGAAAATA CTGAAGGAAG CCCTCAAGAG GAAGGAATGG AAGTAGAAAG CCTAAAACTG 
AGGCTGGAAA AGAAACAGAA AAGAGAGCCA GTTACCAAGA CAAAGAAACA AACTACATTG 
CCATTCAAAC CAATCAAGAA AGCAAAGAAA AGAAACCCCT GGTCTGATTC AGAATCAGAC 
ATGAGCAGTA ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAAAAGAGTC GCGGAGAGCA 
GCAGCAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAGATTTTTC AGATTTTGAT 
GAAGACACTC AAGATGATTT TGTCCCATCA AATGCTAGTC CACCTAAGAT CCAAACTTCC 
CCAAAACATA GTAACAAAGA ACTGAAGCCA CTGAAGAGTG CCTCATCAGT AACAGAGCTT 
GATGCTGATG ATGCTGAGGA TAATGTACCA CCTCCTCCAA GCTCTCCTGC TGCTGATTTC 
CCAGCTGTAA CTGAAACCAT AAACCCTGTT CCTAAAAAGA ATGTGACAGC AAAGAAGACA 
GCAGCAAAAA GTCAGTCTCC CACCTCCACT ACAGGCGCCA AGAAAAGGGC TGCACCAAAA 
AGAACCAAAA AGGACCCAGA CTTGGATTCT GATGTCTCTA AAAAGCCTAA TGCCCCTAAA 
ACCAAGACTC GCCGCAAAAG GAAGCCATCC TCCTCTGATG ATTCGGACTC TAATTTTGAG 
AAACTGATCT CCAAAGCAGT CACAAGCAAG AAACCCAAGG AGGAAAGCGA TGATTTCCAT 
CTGGACTTGG ACTCAGCTGT GGGTTCTCGG GCAAAATCTG GAAGAACAAA GAAACCTATA 
AAGTACCTTG AAGAATCAGA TGAAGATGAT TTCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012041458.1
CDS	142..4740
Translation	MEVSPLQPVNENMQVSKTKKNEDAKKRLSVERIYQKKTQLEHILLRPDTYIGSVESVTQQMWVYDEDTGINYREVTYVPGLYKIFDEILVNAADNKQRDPKMSCIRITIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDEEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGKAGEMELKPFSGEDYTCITFHPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVYLNGNRLPVKGFRSYVDLYLKDKVDETGNPLKIIHEQVNHRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVTKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQVKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQIQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEMAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTHFMEDRRQRKLLGLPDDYLYGQATTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPAKDDHTLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRRLMDGEEPLPMLPSYKNFKGTIEELAPNQYVISGEVAILNSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMTEEKLAEAERVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTALDILRDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNEKQSEKSDSATDSGPTFNYLLDMPLWYLTKEKKDELCKLRNEKEQELETLKRKSPSDLWKEDLAAFVEELEVVEAKEKQDEQIGLPGKGGKAKGKKTQMVEILPSPSGKRVIPRVTEEMKAEAEKKIKKKIKSENTEGSPQEEGMEVESLKLRLEKKQKREPVTKTKKQTTLPFKPIKKAKKRNPWSDSESDMSSNESNFDVPPREKESRRAAAKTKFTMDLDSDEDFSDFDEDTQDDFVPSNASPPKIQTSPKHSNKELKPLKSASSVTELDADDAEDNVPPPPSSPAADFPAVTETINPVPKKNVTAKKTAAKSQSPTSTTGAKKRAAPKRTKKDPDLDSDVSKKPNAPKTKTRRKRKPSSSDDSDSNFEKLISKAVTSKKPKEESDDFHLDLDSAVGSRAKSGRTKKPIKYLEESDEDDFF

Target ORF information:

RefSeq Version	XM_012186068.2
Organism	Ovis aries(sheep)
Definition	Ovis aries topoisomerase (DNA) II alpha (TOP2A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012186068.2

ORF Insert Sequence:

ATGGAGGTGT CGCCACTACA GCCTGTAAAC GAAAATATGC AAGTCAGCAA AACAAAGAAA 
AATGAAGATG CTAAGAAAAG ATTGTCTGTT GAAAGAATCT ATCAAAAGAA AACACAACTG 
GAACATATCC TGCTCCGCCC AGACACCTAC ATTGGCTCTG TGGAATCAGT GACCCAGCAA 
ATGTGGGTTT ACGATGAAGA TACTGGCATT AACTATAGGG AAGTCACTTA TGTTCCTGGT 
TTGTACAAAA TCTTTGATGA GATTCTAGTC AATGCTGCAG ACAACAAACA AAGGGACCCA 
AAAATGTCTT GTATTAGAAT CACAATTGAC CCGGAAAACA ATTTAATTAG TATATGGAAT 
AATGGAAAAG GTATTCCTGT TGTCGAACAC AAAGTTGAGA AGATGTATGT CCCAGCTCTC 
ATCTTTGGAC AGCTCCTAAC TTCTAGCAAC TATGATGATG AAGAAAAGAA AGTAACAGGT 
GGTCGAAATG GCTATGGAGC CAAACTGTGT AACATATTCA GTACCAAATT TACTGTGGAA 
ACAGCAAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA CATGGGGAAA 
GCTGGTGAGA TGGAACTCAA GCCCTTTAGT GGAGAAGATT ATACATGTAT TACCTTCCAC 
CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTAGACAAAG ATATTGTTGC ACTGATGGTT 
AGAAGAGCAT ATGATATTGC TGGATCCACT AAAGATGTCA AGGTCTATCT CAATGGAAAT 
AGGCTTCCAG TGAAAGGATT CCGTAGCTAT GTAGATCTGT ATCTGAAGGA CAAGGTAGAT 
GAAACTGGCA ATCCATTGAA AATTATCCAT GAACAAGTAA ACCACAGGTG GGAAGTATGT 
TTAACAATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACTTCC 
AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT 
GTGAAGAAAA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTAAA AAATCACATG 
TGGATTTTTG TGAATGCCTT AATTGAAAAC CCAACCTTCG ACTCCCAGAC AAAAGAGAAC 
ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAGC TGAGTGAAAA ATTCATCAAA 
GCTGCAATTG GCTGTGGTAT TGTAGAAAGT ATACTAAATT GGGTGAAATT TAAGGCCCAA 
ATCCAGTTAA ACAAGAAGTG TTCTGCTGTA AAACATAACA GAATCAAAGG AATTCCCAAA 
CTTGATGATG CCAATGATGC AGGAGGCCGA AACTCTACTG AGTGTACACT TATCCTGACA 
GAGGGAGACT CAGCTAAAAC TTTAGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAG 
TATGGGGTTT TCCCTCTTAG AGGAAAAATA CTCAATGTAC GAGAAGCTTC TCATAAACAG 
ATAATGGAAA ATGCTGAAAT TAACAATATA ATCAAGATTG TGGGTCTTCA GTACAAGAAA 
AACTATGAAG ATGAAGATTC ATTGAAGACT CTACGTTATG GAAAGATAAT GATTATGACA 
GATCAGGACC AAGACGGCTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCATAAC 
TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCTAT TGTAAAGGTG 
TCTAAAAACA AGCAAGAAAT GGCATTCTAT AGCCTTCCTG AGTTTGAAGA ATGGAAGAGT 
TCTACACCAA ATCATAAAAA ATGGAAAGTC AAGTATTACA AAGGTTTGGG TACCAGCACA 
TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA ATTCAAATAT 
TCCGGTCCCG AAGATGATGC TGCAATCAGC CTGGCCTTTA GCAAAAAGCA GATAGATGAT 
CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGAC AACGAAAGTT ACTTGGCCTT 
CCTGATGATT ATTTGTATGG GCAAGCTACC ACATATCTGA CATACAATGA CTTCATAAAC 
AAGGAACTTA TCCTGTTCTC AAATTCTGAT AATGAAAGAT CTATCCCATC TATGGTGGAT 
GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACATGTT TCAAACGGAA TGACAAGCGA 
GAGGTGAAGG TTGCCCAGTT AGCTGGGTCA GTAGCTGAGA TGTCCTCTTA TCATCATGGT 
GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ACTTTGTGGG CAGCAATAAT 
CTGAACCTTT TGCAGCCCAT TGGTCAGTTT GGTACCAGGT TGCATGGTGG CAAGGACTCT 
GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTGTT ATTTCCAGCA 
AAAGATGACC ATACTTTAAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG 
TACATTCCTA TTATACCCAT GGTGTTGATA AATGGCGCTG AAGGAATTGG TACTGGGTGG 
TCCTGCAAAA TCCCCAACTT TGATGTTCGT GAAGTTGTGA ATAACATCAG ACGTTTGATG 
GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAG 
GAGCTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTGG CTATTCTTAA TTCCACAACC 
ATTGAAATCT CAGAGCTTCC CATCAGAACG TGGACCCAGA CGTATAAAGA ACAGGTTCTT 
GAGCCCATGT TGAATGGCAC TGAGAAGACA CCCCCTCTCA TAACAGATTA TAGGGAATAC 
CACACAGACA CCACTGTGAA GTTTGTTGTT AAGATGACTG AAGAAAAACT GGCAGAGGCA 
GAGAGAGTTG GACTACACAA AGTCTTTAAA CTCCAAACTA GTCTCACATG CAACTCCATG 
GTCCTCTTTG ACCATGTAGG CTGTTTAAAG AAATACGACA CAGCTTTGGA TATTCTAAGA 
GACTTCTTTG AACTCAGGCT TAAATATTAT GGATTAAGAA AAGAATGGCT TCTAGGAATG 
CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT 
GGCAAAATAA TCATTGAAAA TAAGCCCAAG AAAGAGTTAA TTAAAGTTCT GATTCAGAGA 
GGATATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA 
GAAGAAAATG AAGAGAGTGA CAATGAAAAG CAAAGTGAAA AGAGTGACTC TGCAACAGAT 
TCTGGACCCA CCTTCAACTA CCTTCTTGAC ATGCCTCTTT GGTATCTAAC CAAGGAAAAG 
AAAGATGAAC TGTGCAAACT AAGAAATGAA AAAGAGCAAG AGCTAGAAAC ATTAAAGAGA 
AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTGCTT TTGTTGAAGA GCTGGAGGTT 
GTTGAAGCAA AGGAAAAACA AGATGAACAA ATAGGACTTC CTGGGAAAGG TGGGAAAGCA 
AAGGGGAAAA AAACACAAAT GGTAGAAATT TTGCCTTCTC CAAGTGGAAA AAGAGTCATC 
CCCCGAGTGA CTGAAGAGAT GAAAGCAGAG GCAGAAAAGA AAATTAAAAA GAAAATTAAG 
AGTGAAAATA CTGAAGGAAG CCCTCAAGAG GAAGGAATGG AAGTAGAAAG CCTAAAACTG 
AGGCTGGAAA AGAAACAGAA AAGAGAGCCA GTTACCAAGA CAAAGAAACA AACTACATTG 
CCATTCAAAC CAATCAAGAA AGCAAAGAAA AGAAACCCCT GGTCTGATTC AGAATCAGAC 
ATGAGCAGTA ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAAAAGAGTC GCGGAGAGCA 
GCAGCAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAGATTTTTC AGATTTTGAT 
GAAGACACTC AAGATGATTT TGTCCCATCA AATGCTAGTC CACCTAAGAT CCAAACTTCC 
CCAAAACATA GTAACAAAGA ACTGAAGCCA CTGAAGAGTG CCTCATCAGT AACAGAGCTT 
GATGCTGATG ATGCTGAGGA TAATGTACCA CCTCCTCCAA GCTCTCCTGC TGCTGATTTC 
CCAGCTGTAA CTGAAACCAT AAACCCTGTT CCTAAAAAGA ATGTGACAGC AAAGAAGACA 
GCAGCAAAAA GTCAGTCTCC CACCTCCACT ACAGGCGCCA AGAAAAGGGC TGCACCAAAA 
AGAACCAAAA AGGACCCAGA CTTGGATTCT GATGTCTCTA AAAAGCCTAA TGCCCCTAAA 
ACCAAGACTC GCCGCAAAAG GAAGCCATCC TCCTCTGATG ATTCGGACTC TAATTTTGAG 
AAACTGATCT CCAAAGCAGT CACAAGCAAG AAACCCAAGG AGGAAAGCGA TGATTTCCAT 
CTGGACTTGG ACTCAGCTGT GGGTTCTCGG GCAAAATCTG GAAGAACAAA GAAACCTATA 
AAGTACCTTG AAGAATCAGA TGAAGATGAT TTCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP2A ( XM_012186068.3 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012041458.1 Ovis aries DNA topoisomerase II alpha (TOP2A), mRNA.

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CloneID	OSm29655
Clone ID Related Accession (Same CDS sequence)	XM_012186068.2 , XM_012186068.3
Accession Version	XM_012186068.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	DNA topoisomerase 2-alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040262.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_012186068.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGT CGCCACTACA GCCTGTAAAC GAAAATATGC AAGTCAGCAA AACAAAGAAA 
AATGAAGATG CTAAGAAAAG ATTGTCTGTT GAAAGAATCT ATCAAAAGAA AACACAACTG 
GAACATATCC TGCTCCGCCC AGACACCTAC ATTGGCTCTG TGGAATCAGT GACCCAGCAA 
ATGTGGGTTT ACGATGAAGA TACTGGCATT AACTATAGGG AAGTCACTTA TGTTCCTGGT 
TTGTACAAAA TCTTTGATGA GATTCTAGTC AATGCTGCAG ACAACAAACA AAGGGACCCA 
AAAATGTCTT GTATTAGAAT CACAATTGAC CCGGAAAACA ATTTAATTAG TATATGGAAT 
AATGGAAAAG GTATTCCTGT TGTCGAACAC AAAGTTGAGA AGATGTATGT CCCAGCTCTC 
ATCTTTGGAC AGCTCCTAAC TTCTAGCAAC TATGATGATG AAGAAAAGAA AGTAACAGGT 
GGTCGAAATG GCTATGGAGC CAAACTGTGT AACATATTCA GTACCAAATT TACTGTGGAA 
ACAGCAAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA CATGGGGAAA 
GCTGGTGAGA TGGAACTCAA GCCCTTTAGT GGAGAAGATT ATACATGTAT TACCTTCCAC 
CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTAGACAAAG ATATTGTTGC ACTGATGGTT 
AGAAGAGCAT ATGATATTGC TGGATCCACT AAAGATGTCA AGGTCTATCT CAATGGAAAT 
AGGCTTCCAG TGAAAGGATT CCGTAGCTAT GTAGATCTGT ATCTGAAGGA CAAGGTAGAT 
GAAACTGGCA ATCCATTGAA AATTATCCAT GAACAAGTAA ACCACAGGTG GGAAGTATGT 
TTAACAATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACTTCC 
AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT 
GTGAAGAAAA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTAAA AAATCACATG 
TGGATTTTTG TGAATGCCTT AATTGAAAAC CCAACCTTCG ACTCCCAGAC AAAAGAGAAC 
ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAGC TGAGTGAAAA ATTCATCAAA 
GCTGCAATTG GCTGTGGTAT TGTAGAAAGT ATACTAAATT GGGTGAAATT TAAGGCCCAA 
ATCCAGTTAA ACAAGAAGTG TTCTGCTGTA AAACATAACA GAATCAAAGG AATTCCCAAA 
CTTGATGATG CCAATGATGC AGGAGGCCGA AACTCTACTG AGTGTACACT TATCCTGACA 
GAGGGAGACT CAGCTAAAAC TTTAGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAG 
TATGGGGTTT TCCCTCTTAG AGGAAAAATA CTCAATGTAC GAGAAGCTTC TCATAAACAG 
ATAATGGAAA ATGCTGAAAT TAACAATATA ATCAAGATTG TGGGTCTTCA GTACAAGAAA 
AACTATGAAG ATGAAGATTC ATTGAAGACT CTACGTTATG GAAAGATAAT GATTATGACA 
GATCAGGACC AAGACGGCTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCATAAC 
TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCTAT TGTAAAGGTG 
TCTAAAAACA AGCAAGAAAT GGCATTCTAT AGCCTTCCTG AGTTTGAAGA ATGGAAGAGT 
TCTACACCAA ATCATAAAAA ATGGAAAGTC AAGTATTACA AAGGTTTGGG TACCAGCACA 
TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA ATTCAAATAT 
TCCGGTCCCG AAGATGATGC TGCAATCAGC CTGGCCTTTA GCAAAAAGCA GATAGATGAT 
CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGAC AACGAAAGTT ACTTGGCCTT 
CCTGATGATT ATTTGTATGG GCAAGCTACC ACATATCTGA CATACAATGA CTTCATAAAC 
AAGGAACTTA TCCTGTTCTC AAATTCTGAT AATGAAAGAT CTATCCCATC TATGGTGGAT 
GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACATGTT TCAAACGGAA TGACAAGCGA 
GAGGTGAAGG TTGCCCAGTT AGCTGGGTCA GTAGCTGAGA TGTCCTCTTA TCATCATGGT 
GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ACTTTGTGGG CAGCAATAAT 
CTGAACCTTT TGCAGCCCAT TGGTCAGTTT GGTACCAGGT TGCATGGTGG CAAGGACTCT 
GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTGTT ATTTCCAGCA 
AAAGATGACC ATACTTTAAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG 
TACATTCCTA TTATACCCAT GGTGTTGATA AATGGCGCTG AAGGAATTGG TACTGGGTGG 
TCCTGCAAAA TCCCCAACTT TGATGTTCGT GAAGTTGTGA ATAACATCAG ACGTTTGATG 
GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAG 
GAGCTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTGG CTATTCTTAA TTCCACAACC 
ATTGAAATCT CAGAGCTTCC CATCAGAACG TGGACCCAGA CGTATAAAGA ACAGGTTCTT 
GAGCCCATGT TGAATGGCAC TGAGAAGACA CCCCCTCTCA TAACAGATTA TAGGGAATAC 
CACACAGACA CCACTGTGAA GTTTGTTGTT AAGATGACTG AAGAAAAACT GGCAGAGGCA 
GAGAGAGTTG GACTACACAA AGTCTTTAAA CTCCAAACTA GTCTCACATG CAACTCCATG 
GTCCTCTTTG ACCATGTAGG CTGTTTAAAG AAATACGACA CAGCTTTGGA TATTCTAAGA 
GACTTCTTTG AACTCAGGCT TAAATATTAT GGATTAAGAA AAGAATGGCT TCTAGGAATG 
CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT 
GGCAAAATAA TCATTGAAAA TAAGCCCAAG AAAGAGTTAA TTAAAGTTCT GATTCAGAGA 
GGATATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA 
GAAGAAAATG AAGAGAGTGA CAATGAAAAG CAAAGTGAAA AGAGTGACTC TGCAACAGAT 
TCTGGACCCA CCTTCAACTA CCTTCTTGAC ATGCCTCTTT GGTATCTAAC CAAGGAAAAG 
AAAGATGAAC TGTGCAAACT AAGAAATGAA AAAGAGCAAG AGCTAGAAAC ATTAAAGAGA 
AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTGCTT TTGTTGAAGA GCTGGAGGTT 
GTTGAAGCAA AGGAAAAACA AGATGAACAA ATAGGACTTC CTGGGAAAGG TGGGAAAGCA 
AAGGGGAAAA AAACACAAAT GGTAGAAATT TTGCCTTCTC CAAGTGGAAA AAGAGTCATC 
CCCCGAGTGA CTGAAGAGAT GAAAGCAGAG GCAGAAAAGA AAATTAAAAA GAAAATTAAG 
AGTGAAAATA CTGAAGGAAG CCCTCAAGAG GAAGGAATGG AAGTAGAAAG CCTAAAACTG 
AGGCTGGAAA AGAAACAGAA AAGAGAGCCA GTTACCAAGA CAAAGAAACA AACTACATTG 
CCATTCAAAC CAATCAAGAA AGCAAAGAAA AGAAACCCCT GGTCTGATTC AGAATCAGAC 
ATGAGCAGTA ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAAAAGAGTC GCGGAGAGCA 
GCAGCAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAGATTTTTC AGATTTTGAT 
GAAGACACTC AAGATGATTT TGTCCCATCA AATGCTAGTC CACCTAAGAT CCAAACTTCC 
CCAAAACATA GTAACAAAGA ACTGAAGCCA CTGAAGAGTG CCTCATCAGT AACAGAGCTT 
GATGCTGATG ATGCTGAGGA TAATGTACCA CCTCCTCCAA GCTCTCCTGC TGCTGATTTC 
CCAGCTGTAA CTGAAACCAT AAACCCTGTT CCTAAAAAGA ATGTGACAGC AAAGAAGACA 
GCAGCAAAAA GTCAGTCTCC CACCTCCACT ACAGGCGCCA AGAAAAGGGC TGCACCAAAA 
AGAACCAAAA AGGACCCAGA CTTGGATTCT GATGTCTCTA AAAAGCCTAA TGCCCCTAAA 
ACCAAGACTC GCCGCAAAAG GAAGCCATCC TCCTCTGATG ATTCGGACTC TAATTTTGAG 
AAACTGATCT CCAAAGCAGT CACAAGCAAG AAACCCAAGG AGGAAAGCGA TGATTTCCAT 
CTGGACTTGG ACTCAGCTGT GGGTTCTCGG GCAAAATCTG GAAGAACAAA GAAACCTATA 
AAGTACCTTG AAGAATCAGA TGAAGATGAT TTCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012041458.1
CDS	120..4718
Translation	MEVSPLQPVNENMQVSKTKKNEDAKKRLSVERIYQKKTQLEHILLRPDTYIGSVESVTQQMWVYDEDTGINYREVTYVPGLYKIFDEILVNAADNKQRDPKMSCIRITIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDEEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGKAGEMELKPFSGEDYTCITFHPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVYLNGNRLPVKGFRSYVDLYLKDKVDETGNPLKIIHEQVNHRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVTKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQVKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQIQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEMAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTHFMEDRRQRKLLGLPDDYLYGQATTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPAKDDHTLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRRLMDGEEPLPMLPSYKNFKGTIEELAPNQYVISGEVAILNSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMTEEKLAEAERVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTALDILRDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNEKQSEKSDSATDSGPTFNYLLDMPLWYLTKEKKDELCKLRNEKEQELETLKRKSPSDLWKEDLAAFVEELEVVEAKEKQDEQIGLPGKGGKAKGKKTQMVEILPSPSGKRVIPRVTEEMKAEAEKKIKKKIKSENTEGSPQEEGMEVESLKLRLEKKQKREPVTKTKKQTTLPFKPIKKAKKRNPWSDSESDMSSNESNFDVPPREKESRRAAAKTKFTMDLDSDEDFSDFDEDTQDDFVPSNASPPKIQTSPKHSNKELKPLKSASSVTELDADDAEDNVPPPPSSPAADFPAVTETINPVPKKNVTAKKTAAKSQSPTSTTGAKKRAAPKRTKKDPDLDSDVSKKPNAPKTKTRRKRKPSSSDDSDSNFEKLISKAVTSKKPKEESDDFHLDLDSAVGSRAKSGRTKKPIKYLEESDEDDFF

Target ORF information:

RefSeq Version	XM_012186068.3
Organism	Ovis aries(sheep)
Definition	Ovis aries DNA topoisomerase II alpha (TOP2A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012186068.3

ORF Insert Sequence:

ATGGAGGTGT CGCCACTACA GCCTGTAAAC GAAAATATGC AAGTCAGCAA AACAAAGAAA 
AATGAAGATG CTAAGAAAAG ATTGTCTGTT GAAAGAATCT ATCAAAAGAA AACACAACTG 
GAACATATCC TGCTCCGCCC AGACACCTAC ATTGGCTCTG TGGAATCAGT GACCCAGCAA 
ATGTGGGTTT ACGATGAAGA TACTGGCATT AACTATAGGG AAGTCACTTA TGTTCCTGGT 
TTGTACAAAA TCTTTGATGA GATTCTAGTC AATGCTGCAG ACAACAAACA AAGGGACCCA 
AAAATGTCTT GTATTAGAAT CACAATTGAC CCGGAAAACA ATTTAATTAG TATATGGAAT 
AATGGAAAAG GTATTCCTGT TGTCGAACAC AAAGTTGAGA AGATGTATGT CCCAGCTCTC 
ATCTTTGGAC AGCTCCTAAC TTCTAGCAAC TATGATGATG AAGAAAAGAA AGTAACAGGT 
GGTCGAAATG GCTATGGAGC CAAACTGTGT AACATATTCA GTACCAAATT TACTGTGGAA 
ACAGCAAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA CATGGGGAAA 
GCTGGTGAGA TGGAACTCAA GCCCTTTAGT GGAGAAGATT ATACATGTAT TACCTTCCAC 
CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTAGACAAAG ATATTGTTGC ACTGATGGTT 
AGAAGAGCAT ATGATATTGC TGGATCCACT AAAGATGTCA AGGTCTATCT CAATGGAAAT 
AGGCTTCCAG TGAAAGGATT CCGTAGCTAT GTAGATCTGT ATCTGAAGGA CAAGGTAGAT 
GAAACTGGCA ATCCATTGAA AATTATCCAT GAACAAGTAA ACCACAGGTG GGAAGTATGT 
TTAACAATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACTTCC 
AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT 
GTGAAGAAAA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTAAA AAATCACATG 
TGGATTTTTG TGAATGCCTT AATTGAAAAC CCAACCTTCG ACTCCCAGAC AAAAGAGAAC 
ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAGC TGAGTGAAAA ATTCATCAAA 
GCTGCAATTG GCTGTGGTAT TGTAGAAAGT ATACTAAATT GGGTGAAATT TAAGGCCCAA 
ATCCAGTTAA ACAAGAAGTG TTCTGCTGTA AAACATAACA GAATCAAAGG AATTCCCAAA 
CTTGATGATG CCAATGATGC AGGAGGCCGA AACTCTACTG AGTGTACACT TATCCTGACA 
GAGGGAGACT CAGCTAAAAC TTTAGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAG 
TATGGGGTTT TCCCTCTTAG AGGAAAAATA CTCAATGTAC GAGAAGCTTC TCATAAACAG 
ATAATGGAAA ATGCTGAAAT TAACAATATA ATCAAGATTG TGGGTCTTCA GTACAAGAAA 
AACTATGAAG ATGAAGATTC ATTGAAGACT CTACGTTATG GAAAGATAAT GATTATGACA 
GATCAGGACC AAGACGGCTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCATAAC 
TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCTAT TGTAAAGGTG 
TCTAAAAACA AGCAAGAAAT GGCATTCTAT AGCCTTCCTG AGTTTGAAGA ATGGAAGAGT 
TCTACACCAA ATCATAAAAA ATGGAAAGTC AAGTATTACA AAGGTTTGGG TACCAGCACA 
TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA ATTCAAATAT 
TCCGGTCCCG AAGATGATGC TGCAATCAGC CTGGCCTTTA GCAAAAAGCA GATAGATGAT 
CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGAC AACGAAAGTT ACTTGGCCTT 
CCTGATGATT ATTTGTATGG GCAAGCTACC ACATATCTGA CATACAATGA CTTCATAAAC 
AAGGAACTTA TCCTGTTCTC AAATTCTGAT AATGAAAGAT CTATCCCATC TATGGTGGAT 
GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACATGTT TCAAACGGAA TGACAAGCGA 
GAGGTGAAGG TTGCCCAGTT AGCTGGGTCA GTAGCTGAGA TGTCCTCTTA TCATCATGGT 
GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ACTTTGTGGG CAGCAATAAT 
CTGAACCTTT TGCAGCCCAT TGGTCAGTTT GGTACCAGGT TGCATGGTGG CAAGGACTCT 
GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTGTT ATTTCCAGCA 
AAAGATGACC ATACTTTAAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG 
TACATTCCTA TTATACCCAT GGTGTTGATA AATGGCGCTG AAGGAATTGG TACTGGGTGG 
TCCTGCAAAA TCCCCAACTT TGATGTTCGT GAAGTTGTGA ATAACATCAG ACGTTTGATG 
GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAG 
GAGCTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTGG CTATTCTTAA TTCCACAACC 
ATTGAAATCT CAGAGCTTCC CATCAGAACG TGGACCCAGA CGTATAAAGA ACAGGTTCTT 
GAGCCCATGT TGAATGGCAC TGAGAAGACA CCCCCTCTCA TAACAGATTA TAGGGAATAC 
CACACAGACA CCACTGTGAA GTTTGTTGTT AAGATGACTG AAGAAAAACT GGCAGAGGCA 
GAGAGAGTTG GACTACACAA AGTCTTTAAA CTCCAAACTA GTCTCACATG CAACTCCATG 
GTCCTCTTTG ACCATGTAGG CTGTTTAAAG AAATACGACA CAGCTTTGGA TATTCTAAGA 
GACTTCTTTG AACTCAGGCT TAAATATTAT GGATTAAGAA AAGAATGGCT TCTAGGAATG 
CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT 
GGCAAAATAA TCATTGAAAA TAAGCCCAAG AAAGAGTTAA TTAAAGTTCT GATTCAGAGA 
GGATATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA 
GAAGAAAATG AAGAGAGTGA CAATGAAAAG CAAAGTGAAA AGAGTGACTC TGCAACAGAT 
TCTGGACCCA CCTTCAACTA CCTTCTTGAC ATGCCTCTTT GGTATCTAAC CAAGGAAAAG 
AAAGATGAAC TGTGCAAACT AAGAAATGAA AAAGAGCAAG AGCTAGAAAC ATTAAAGAGA 
AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTGCTT TTGTTGAAGA GCTGGAGGTT 
GTTGAAGCAA AGGAAAAACA AGATGAACAA ATAGGACTTC CTGGGAAAGG TGGGAAAGCA 
AAGGGGAAAA AAACACAAAT GGTAGAAATT TTGCCTTCTC CAAGTGGAAA AAGAGTCATC 
CCCCGAGTGA CTGAAGAGAT GAAAGCAGAG GCAGAAAAGA AAATTAAAAA GAAAATTAAG 
AGTGAAAATA CTGAAGGAAG CCCTCAAGAG GAAGGAATGG AAGTAGAAAG CCTAAAACTG 
AGGCTGGAAA AGAAACAGAA AAGAGAGCCA GTTACCAAGA CAAAGAAACA AACTACATTG 
CCATTCAAAC CAATCAAGAA AGCAAAGAAA AGAAACCCCT GGTCTGATTC AGAATCAGAC 
ATGAGCAGTA ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAAAAGAGTC GCGGAGAGCA 
GCAGCAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAGATTTTTC AGATTTTGAT 
GAAGACACTC AAGATGATTT TGTCCCATCA AATGCTAGTC CACCTAAGAT CCAAACTTCC 
CCAAAACATA GTAACAAAGA ACTGAAGCCA CTGAAGAGTG CCTCATCAGT AACAGAGCTT 
GATGCTGATG ATGCTGAGGA TAATGTACCA CCTCCTCCAA GCTCTCCTGC TGCTGATTTC 
CCAGCTGTAA CTGAAACCAT AAACCCTGTT CCTAAAAAGA ATGTGACAGC AAAGAAGACA 
GCAGCAAAAA GTCAGTCTCC CACCTCCACT ACAGGCGCCA AGAAAAGGGC TGCACCAAAA 
AGAACCAAAA AGGACCCAGA CTTGGATTCT GATGTCTCTA AAAAGCCTAA TGCCCCTAAA 
ACCAAGACTC GCCGCAAAAG GAAGCCATCC TCCTCTGATG ATTCGGACTC TAATTTTGAG 
AAACTGATCT CCAAAGCAGT CACAAGCAAG AAACCCAAGG AGGAAAGCGA TGATTTCCAT 
CTGGACTTGG ACTCAGCTGT GGGTTCTCGG GCAAAATCTG GAAGAACAAA GAAACCTATA 
AAGTACCTTG AAGAATCAGA TGAAGATGAT TTCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP2A ( XM_012186069.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012041459.1 Ovis aries topoisomerase (DNA) II alpha (TOP2A), transcript variant X2, mRNA.

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CloneID	OSm29656
Clone ID Related Accession (Same CDS sequence)	XM_012186069.2
Accession Version	XM_012186069.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	DNA topoisomerase 2-alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639020.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012186069.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGT CGCCACTACA GCCTGTAAAC GAAAATATGC AAGTCAGCAA AACAAAGAAA 
AATGAAGATG CTAAGAAAAG ATTGTCTGTT GAAAGAATCT ATCAAAAGAA AACACAACTG 
GAACATATCC TGCTCCGCCC AGACACCTAC ATTGGCTCTG TGGAATCAGT GACCCAGCAA 
ATGTGGGTTT ACGATGAAGA TACTGGCATT AACTATAGGG AAGTCACTTA TGTTCCTGGT 
TTGTACAAAA TCTTTGATGA GATTCTAGTC AATGCTGCAG ACAACAAACA AAGGGACCCA 
AAAATGTCTT GTATTAGAAT CACAATTGAC CCGGAAAACA ATTTAATTAG TATATGGAAT 
AATGGAAAAG GTATTCCTGT TGTCGAACAC AAAGTTGAGA AGATGTATGT CCCAGCTCTC 
ATCTTTGGAC AGCTCCTAAC TTCTAGCAAC TATGATGATG AAGAAAAGAA AGTAACAGGT 
GGTCGAAATG GCTATGGAGC CAAACTGTGT AACATATTCA GTACCAAATT TACTGTGGAA 
ACAGCAAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA CATGGGGAAA 
GCTGGTGAGA TGGAACTCAA GCCCTTTAGT GGAGAAGATT ATACATGTAT TACCTTCCAC 
CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTAGACAAAG ATATTGTTGC ACTGATGGTT 
AGAAGAGCAT ATGATATTGC TGGATCCACT AAAGATGTCA AGGTCTATCT CAATGGAAAT 
AGGCTTCCAG TGAAAGGATT CCGTAGCTAT GTAGATCTGT ATCTGAAGGA CAAGGTAGAT 
GAAACTGGCA ATCCATTGAA AATTATCCAT GAACAAGTAA ACCACAGGTG GGAAGTATGT 
TTAACAATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACTTCC 
AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT 
GTGAAGAAAA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTAAA AAATCACATG 
TGGATTTTTG TGAATGCCTT AATTGAAAAC CCAACCTTCG ACTCCCAGAC AAAAGAGAAC 
ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAGC TGAGTGAAAA ATTCATCAAA 
GCTGCAATTG GCTGTGGTAT TGTAGAAAGT ATACTAAATT GGGTGAAATT TAAGGCCCAA 
ATCCAGTTAA ACAAGAAGTG TTCTGCTGTA AAACATAACA GAATCAAAGG AATTCCCAAA 
CTTGATGATG CCAATGATGC AGGAGGCCGA AACTCTACTG AGTGTACACT TATCCTGACA 
GAGGGAGACT CAGCTAAAAC TTTAGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAG 
TATGGGGTTT TCCCTCTTAG AGGAAAAATA CTCAATGTAC GAGAAGCTTC TCATAAACAG 
ATAATGGAAA ATGCTGAAAT TAACAATATA ATCAAGATTG TGGGTCTTCA GTACAAGAAA 
AACTATGAAG ATGAAGATTC ATTGAAGACT CTACGTTATG GAAAGATAAT GATTATGACA 
GATCAGGACC AAGACGGCTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCATAAC 
TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCTAT TGTAAAGGTG 
TCTAAAAACA AGCAAGAAAT GGCATTCTAT AGCCTTCCTG AGTTTGAAGA ATGGAAGAGT 
TCTACACCAA ATCATAAAAA ATGGAAAGTC AAGTATTACA AAGGTTTGGG TACCAGCACA 
TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA ATTCAAATAT 
TCCGGTCCCG AAGATGATGC TGCAATCAGC CTGGCCTTTA GCAAAAAGCA GATAGATGAT 
CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGAC AACGAAAGTT ACTTGGCCTT 
CCTGATGATT ATTTGTATGG GCAAGCTACC ACATATCTGA CATACAATGA CTTCATAAAC 
AAGGAACTTA TCCTGTTCTC AAATTCTGAT AATGAAAGAT CTATCCCATC TATGGTGGAT 
GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACATGTT TCAAACGGAA TGACAAGCGA 
GAGGTGAAGG TTGCCCAGTT AGCTGGGTCA GTAGCTGAGA TGTCCTCTTA TCATCATGGT 
GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ACTTTGTGGG CAGCAATAAT 
CTGAACCTTT TGCAGCCCAT TGGTCAGTTT GGTACCAGGT TGCATGGTGG CAAGGACTCT 
GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTGTT ATTTCCAGCA 
AAAGATGACC ATACTTTAAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG 
TACATTCCTA TTATACCCAT GGTGTTGATA AATGGCGCTG AAGGAATTGG TACTGGGTGG 
TCCTGCAAAA TCCCCAACTT TGATGTTCGT GAAGTTGTGA ATAACATCAG ACGTTTGATG 
GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAG 
GAGCTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTGG CTATTCTTAA TTCCACAACC 
ATTGAAATCT CAGAGCTTCC CATCAGAACG TGGACCCAGA CGTATAAAGA ACAGGTTCTT 
GAGCCCATGT TGAATGGCAC TGAGAAGACA CCCCCTCTCA TAACAGATTA TAGGGAATAC 
CACACAGACA CCACTGTGAA GTTTGTTGTT AAGATGACTG AAGAAAAACT GGCAGAGGCA 
GAGAGAGTTG GACTACACAA AGTCTTTAAA CTCCAAACTA GTCTCACATG CAACTCCATG 
GTCCTCTTTG ACCATGTAGG CTGTTTAAAG AAATACGACA CAGCTTTGGA TATTCTAAGA 
GACTTCTTTG AACTCAGGCT TAAATATTAT GGATTAAGAA AAGAATGGCT TCTAGGAATG 
CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT 
GGCAAAATAA TCATTGAAAA TAAGCCCAAG AAAGAGTTAA TTAAAGTTCT GATTCAGAGA 
GGATATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA 
GAAGAAAATG AAGAGAGTGA CAATGAAAAG CAAAGTGAAA AGAGTGACTC TGCAACAGAT 
TCTGGACCCA CCTTCAACTA CCTTCTTGAC ATGCCTCTTT GGTATCTAAC CAAGGAAAAG 
AAAGATGAAC TGTGCAAACT AAGAAATGAA AAAGAGCAAG AGCTAGAAAC ATTAAAGAGA 
AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTGCTT TTGTTGAAGA GCTGGAGGTT 
GTTGAAGCAA AGGAAAAACA AGATGAACAA ATAGGACTTC CTGGGAAAGG TGGGAAAGCA 
AAGGGGAAAA AAACACAAAT GGTAGAAATT TTGCCTTCTC CAAGTGGAAA AAGAGTCATC 
CCCCGAGTGA CTGAAGAGAT GAAAGCAGAG GCAGAAAAGA AAATTAAAAA GAAAATTAAG 
AGTGAAAATA CTGAAGGAAG CCCTCAAGAG GAAGGAATGG AAGTAGAAAG CCTAAAACTG 
AGGCTGGAAA AGAAACAGAA AAGAGAGCCA GTTACCAAGA CAAAGAAACA AACTACATTG 
CCATTCAAAC CAATCAAGAA AGCAAAGAAA AGAAACCCCT GGTCTGATTC AGAATCAGAC 
ATGAGCAGTA ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAAAAGAGTC GCGGAGAGCA 
GCAGCAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAGATTTTTC AGATTTTGAT 
GAAGACACTC AAGATGATTT TGTCCCATCA AATGCTAGTC CACCTAAGAT CCAAACTTCC 
CCAAAACATA GTAACAAAGA ACTGAAGCCA CTGAAGAGTG CCTCATCAGT AACAGAGCTT 
GATGCTGATG ATGCTGAGGA TAATGTACCA CCTCCTCCAA GCTCTCCTGC TGCTGATTTC 
CCAGCTGTAA CTGAAACCAT AAACCCTGTT CCTAAAAAGA ATGTGACAGC AAAGAAGACA 
GCAGCAAAAA GCGCCAAGAA AAGGGCTGCA CCAAAAAGAA CCAAAAAGGA CCCAGACTTG 
GATTCTGATG TCTCTAAAAA GCCTAATGCC CCTAAAACCA AGACTCGCCG CAAAAGGAAG 
CCATCCTCCT CTGATGATTC GGACTCTAAT TTTGAGAAAC TGATCTCCAA AGCAGTCACA 
AGCAAGAAAC CCAAGGAGGA AAGCGATGAT TTCCATCTGG ACTTGGACTC AGCTGTGGGT 
TCTCGGGCAA AATCTGGAAG AACAAAGAAA CCTATAAAGT ACCTTGAAGA ATCAGATGAA 
GATGATTTCT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012041459.1
CDS	143..4717
Translation	MEVSPLQPVNENMQVSKTKKNEDAKKRLSVERIYQKKTQLEHILLRPDTYIGSVESVTQQMWVYDEDTGINYREVTYVPGLYKIFDEILVNAADNKQRDPKMSCIRITIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDEEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGKAGEMELKPFSGEDYTCITFHPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVYLNGNRLPVKGFRSYVDLYLKDKVDETGNPLKIIHEQVNHRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVTKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQVKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQIQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEMAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTHFMEDRRQRKLLGLPDDYLYGQATTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPAKDDHTLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRRLMDGEEPLPMLPSYKNFKGTIEELAPNQYVISGEVAILNSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMTEEKLAEAERVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTALDILRDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNEKQSEKSDSATDSGPTFNYLLDMPLWYLTKEKKDELCKLRNEKEQELETLKRKSPSDLWKEDLAAFVEELEVVEAKEKQDEQIGLPGKGGKAKGKKTQMVEILPSPSGKRVIPRVTEEMKAEAEKKIKKKIKSENTEGSPQEEGMEVESLKLRLEKKQKREPVTKTKKQTTLPFKPIKKAKKRNPWSDSESDMSSNESNFDVPPREKESRRAAAKTKFTMDLDSDEDFSDFDEDTQDDFVPSNASPPKIQTSPKHSNKELKPLKSASSVTELDADDAEDNVPPPPSSPAADFPAVTETINPVPKKNVTAKKTAAKSAKKRAAPKRTKKDPDLDSDVSKKPNAPKTKTRRKRKPSSSDDSDSNFEKLISKAVTSKKPKEESDDFHLDLDSAVGSRAKSGRTKKPIKYLEESDEDDFF

Target ORF information:

RefSeq Version	XM_012186069.2
Organism	Ovis aries(sheep)
Definition	Ovis aries topoisomerase (DNA) II alpha (TOP2A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012186069.2

ORF Insert Sequence:

ATGGAGGTGT CGCCACTACA GCCTGTAAAC GAAAATATGC AAGTCAGCAA AACAAAGAAA 
AATGAAGATG CTAAGAAAAG ATTGTCTGTT GAAAGAATCT ATCAAAAGAA AACACAACTG 
GAACATATCC TGCTCCGCCC AGACACCTAC ATTGGCTCTG TGGAATCAGT GACCCAGCAA 
ATGTGGGTTT ACGATGAAGA TACTGGCATT AACTATAGGG AAGTCACTTA TGTTCCTGGT 
TTGTACAAAA TCTTTGATGA GATTCTAGTC AATGCTGCAG ACAACAAACA AAGGGACCCA 
AAAATGTCTT GTATTAGAAT CACAATTGAC CCGGAAAACA ATTTAATTAG TATATGGAAT 
AATGGAAAAG GTATTCCTGT TGTCGAACAC AAAGTTGAGA AGATGTATGT CCCAGCTCTC 
ATCTTTGGAC AGCTCCTAAC TTCTAGCAAC TATGATGATG AAGAAAAGAA AGTAACAGGT 
GGTCGAAATG GCTATGGAGC CAAACTGTGT AACATATTCA GTACCAAATT TACTGTGGAA 
ACAGCAAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA CATGGGGAAA 
GCTGGTGAGA TGGAACTCAA GCCCTTTAGT GGAGAAGATT ATACATGTAT TACCTTCCAC 
CCTGATTTGT CTAAGTTTAA AATGCAAAGC CTAGACAAAG ATATTGTTGC ACTGATGGTT 
AGAAGAGCAT ATGATATTGC TGGATCCACT AAAGATGTCA AGGTCTATCT CAATGGAAAT 
AGGCTTCCAG TGAAAGGATT CCGTAGCTAT GTAGATCTGT ATCTGAAGGA CAAGGTAGAT 
GAAACTGGCA ATCCATTGAA AATTATCCAT GAACAAGTAA ACCACAGGTG GGAAGTATGT 
TTAACAATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACTTCC 
AAGGGTGGCA GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT 
GTGAAGAAAA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTAAA AAATCACATG 
TGGATTTTTG TGAATGCCTT AATTGAAAAC CCAACCTTCG ACTCCCAGAC AAAAGAGAAC 
ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAGC TGAGTGAAAA ATTCATCAAA 
GCTGCAATTG GCTGTGGTAT TGTAGAAAGT ATACTAAATT GGGTGAAATT TAAGGCCCAA 
ATCCAGTTAA ACAAGAAGTG TTCTGCTGTA AAACATAACA GAATCAAAGG AATTCCCAAA 
CTTGATGATG CCAATGATGC AGGAGGCCGA AACTCTACTG AGTGTACACT TATCCTGACA 
GAGGGAGACT CAGCTAAAAC TTTAGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAG 
TATGGGGTTT TCCCTCTTAG AGGAAAAATA CTCAATGTAC GAGAAGCTTC TCATAAACAG 
ATAATGGAAA ATGCTGAAAT TAACAATATA ATCAAGATTG TGGGTCTTCA GTACAAGAAA 
AACTATGAAG ATGAAGATTC ATTGAAGACT CTACGTTATG GAAAGATAAT GATTATGACA 
GATCAGGACC AAGACGGCTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCATAAC 
TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCTAT TGTAAAGGTG 
TCTAAAAACA AGCAAGAAAT GGCATTCTAT AGCCTTCCTG AGTTTGAAGA ATGGAAGAGT 
TCTACACCAA ATCATAAAAA ATGGAAAGTC AAGTATTACA AAGGTTTGGG TACCAGCACA 
TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA ATTCAAATAT 
TCCGGTCCCG AAGATGATGC TGCAATCAGC CTGGCCTTTA GCAAAAAGCA GATAGATGAT 
CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGAC AACGAAAGTT ACTTGGCCTT 
CCTGATGATT ATTTGTATGG GCAAGCTACC ACATATCTGA CATACAATGA CTTCATAAAC 
AAGGAACTTA TCCTGTTCTC AAATTCTGAT AATGAAAGAT CTATCCCATC TATGGTGGAT 
GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACATGTT TCAAACGGAA TGACAAGCGA 
GAGGTGAAGG TTGCCCAGTT AGCTGGGTCA GTAGCTGAGA TGTCCTCTTA TCATCATGGT 
GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ACTTTGTGGG CAGCAATAAT 
CTGAACCTTT TGCAGCCCAT TGGTCAGTTT GGTACCAGGT TGCATGGTGG CAAGGACTCT 
GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTGTT ATTTCCAGCA 
AAAGATGACC ATACTTTAAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG 
TACATTCCTA TTATACCCAT GGTGTTGATA AATGGCGCTG AAGGAATTGG TACTGGGTGG 
TCCTGCAAAA TCCCCAACTT TGATGTTCGT GAAGTTGTGA ATAACATCAG ACGTTTGATG 
GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACTATTGAG 
GAGCTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTGG CTATTCTTAA TTCCACAACC 
ATTGAAATCT CAGAGCTTCC CATCAGAACG TGGACCCAGA CGTATAAAGA ACAGGTTCTT 
GAGCCCATGT TGAATGGCAC TGAGAAGACA CCCCCTCTCA TAACAGATTA TAGGGAATAC 
CACACAGACA CCACTGTGAA GTTTGTTGTT AAGATGACTG AAGAAAAACT GGCAGAGGCA 
GAGAGAGTTG GACTACACAA AGTCTTTAAA CTCCAAACTA GTCTCACATG CAACTCCATG 
GTCCTCTTTG ACCATGTAGG CTGTTTAAAG AAATACGACA CAGCTTTGGA TATTCTAAGA 
GACTTCTTTG AACTCAGGCT TAAATATTAT GGATTAAGAA AAGAATGGCT TCTAGGAATG 
CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT 
GGCAAAATAA TCATTGAAAA TAAGCCCAAG AAAGAGTTAA TTAAAGTTCT GATTCAGAGA 
GGATATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCCC AGCAAAAGGT TCCAGATGAA 
GAAGAAAATG AAGAGAGTGA CAATGAAAAG CAAAGTGAAA AGAGTGACTC TGCAACAGAT 
TCTGGACCCA CCTTCAACTA CCTTCTTGAC ATGCCTCTTT GGTATCTAAC CAAGGAAAAG 
AAAGATGAAC TGTGCAAACT AAGAAATGAA AAAGAGCAAG AGCTAGAAAC ATTAAAGAGA 
AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTGCTT TTGTTGAAGA GCTGGAGGTT 
GTTGAAGCAA AGGAAAAACA AGATGAACAA ATAGGACTTC CTGGGAAAGG TGGGAAAGCA 
AAGGGGAAAA AAACACAAAT GGTAGAAATT TTGCCTTCTC CAAGTGGAAA AAGAGTCATC 
CCCCGAGTGA CTGAAGAGAT GAAAGCAGAG GCAGAAAAGA AAATTAAAAA GAAAATTAAG 
AGTGAAAATA CTGAAGGAAG CCCTCAAGAG GAAGGAATGG AAGTAGAAAG CCTAAAACTG 
AGGCTGGAAA AGAAACAGAA AAGAGAGCCA GTTACCAAGA CAAAGAAACA AACTACATTG 
CCATTCAAAC CAATCAAGAA AGCAAAGAAA AGAAACCCCT GGTCTGATTC AGAATCAGAC 
ATGAGCAGTA ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAAAAGAGTC GCGGAGAGCA 
GCAGCAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAGATTTTTC AGATTTTGAT 
GAAGACACTC AAGATGATTT TGTCCCATCA AATGCTAGTC CACCTAAGAT CCAAACTTCC 
CCAAAACATA GTAACAAAGA ACTGAAGCCA CTGAAGAGTG CCTCATCAGT AACAGAGCTT 
GATGCTGATG ATGCTGAGGA TAATGTACCA CCTCCTCCAA GCTCTCCTGC TGCTGATTTC 
CCAGCTGTAA CTGAAACCAT AAACCCTGTT CCTAAAAAGA ATGTGACAGC AAAGAAGACA 
GCAGCAAAAA GCGCCAAGAA AAGGGCTGCA CCAAAAAGAA CCAAAAAGGA CCCAGACTTG 
GATTCTGATG TCTCTAAAAA GCCTAATGCC CCTAAAACCA AGACTCGCCG CAAAAGGAAG 
CCATCCTCCT CTGATGATTC GGACTCTAAT TTTGAGAAAC TGATCTCCAA AGCAGTCACA 
AGCAAGAAAC CCAAGGAGGA AAGCGATGAT TTCCATCTGG ACTTGGACTC AGCTGTGGGT 
TCTCGGGCAA AATCTGGAAG AACAAAGAAA CCTATAAAGT ACCTTGAAGA ATCAGATGAA 
GATGATTTCT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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1-732-210-0262

TOP2A cDNA ORF clone, Ovis aries(sheep)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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