LOC112277871 cDNA ORF clone,

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gRNAs

The following LOC112277871 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC112277871 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPn26501	XM_024510667.1 Latest version!	Physcomitrella patens 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase-like (LOC112277871), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC112277871 ( XM_024510667.1 ) cDNA ORF clone, -> XP_024366435.1 Physcomitrella patens 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase-like (LOC112277871), mRNA.

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CloneID	OPn26501
Clone ID Related Accession (Same CDS sequence)	XM_024510667.1
Accession Version	XM_024510667.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037278.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTC ACGTGGTTGG ATATCCCCGC ATGGGCCCCA AGAGGGAGCT CAAGTTTGCT 
CTTGAGTCGA TGTGGGATGG CAAGACCCAG GCAGCTGATT TGGAGCAGGT TGCCAAGGAC 
CTGCGCGCAT CGATCTGGAA GCAGATGTCT GAGGCTGGAG TGAGCATGAT TCCTAGCAAC 
ACATTCTCTT ACTACGACCA GGTGCTGGAC ACTACCGCCA TGGTTGGAGC TGTGCCCGCT 
CGCTACGGAT GGACGGGTGG AGAGATTGGA CACGATGTGT ATTTCTCAAT GGCCCGTGGA 
AACAAGACGA AGCCCGCCAT GGAGATGACC AAGTGGTTCG ATACCAACTA CCACTACATT 
GTGCCAGAGT TGAGCGGTGA GACAAAGTTT GTTTACTCTT CACGCAAGGC CGTGAGCGAG 
TACAAGGAGG CTAAGGAGCT TGGCGTAGAC ACTGTGCCCG TGCTGATAGG TCCAGTGACT 
TATCTGCTGT TGTCCAAGGG TGACAAGAGC GCGCCCAAGG GTTTCAATAC TCTTTCACTT 
CTTGATGCCA TACTTCCCGT GTACAAGGAA GTCATTACAG AGCTGAAGGA AGCTGGTGCG 
TCGTGGATCC AACTGGACGA GCCAAAGTTA GTCATGGACC TTGAAGCTAG CCAATACGAA 
GCTTTTAAGA AGGCATACGC TGTGCTCGGG GAGGTAGCTC AAGTGAAGTT GTTGGTGGAG 
ACATACTTCA CCGACCTGCC TGCCGAGGCC TACAAGACTT TGTGTGACTT GCCAATTGCC 
GGTTTCGGGG TGGACCTCGT CCGCGGCAAC AAAACCTTGA GCCTAATCAA GGAGCACGGT 
CTTCCTGAGG GGAAGACTTT GTTTGCCGGA GTGGTGGACG GAAGGAACAT CTGGGCCAAC 
GACTTGGCTG CCTCTGTGGC CGTGGTTGAG GAATTGCAGG CTAAGCTTGG GAAGGATAAC 
GTAGTTGTCT CAACCTCATG CTCCTTGCTC CATTCCGCAG TGGACCTCAA GAACGAGACA 
AAGTTGGATA GCGAATTGAA GTCCTGGATG GCATTCGCCG CACAGAAGCT GCTGGAGGTA 
GTGGCGGTCG CTAAGGCCGT GTCGGGACAG AAAGACGAGG CTTTCTTCGC GGCTAACGCT 
TCCGCTCAGG AATCGAGGAG GAACTCCCCC CGCGTTCATA ACAAGGCAGT GAAGGAAGCA 
GCCGCTGCTT TGGCCGGTTC GGAGCACCGT CGATCTACCC CGGTATCAAG CCGTCTGGAA 
CAGCAGCAGA AGTACTTGAA CCTGCCAATC CTGCCGACGA CCACGATCGG ATCGTTCCCC 
CAGACGCCAG AGCTCCGCAG GGTCAGGCGT GAGGTGAAGA GCAAGAAGAT CTCAGAGGAG 
GATTATGACA AGGCCATCAA GGCAGAGATT GACAGTGTGG TGAAGCTGCA AGAGGAGCTG 
GACATTGATG TGCTGGTCCA CGGAGAGCCA GAGAGGAATG ATATGGTTGA GTACTTCGGC 
GAGCAGTTGG ATGGTTTCTG CTTCTCAGCC AACGGGTGGG TTCAGTCTTA CGGGTCTCGG 
TGCGTTAAGC CCCCGATTAT CTTCGGTGAC GTGAGCAGGC CGAAGGCCAT GACAGTGTAC 
TGGTCGACTT ACGCTCAGAG CGTGACCAAG AGGCCTATTA AGGGCATGTT GACTGGACCG 
GTTACAATTT TGAACTGGTC TTTCGTGAGG AACGACCAGC CCAGGTCAGA GACCTGTTAC 
CAGATTTCAC TGGCTATCAA GGACGAGGTC GAGGACTTGG AGAAGGCTAA CATTACGGTG 
ATCCAGATCG ATGAGGCTGC CCTTCGCGAG GGACTGCCCC TGCGTAAGTC CGAGCACGCA 
GACTACTTAA GGTGGGCTGT GCACGGGTTC AGAATTACGA CTTGTGGAGT TAAGGATACC 
ACCCAGATTC ACACCCACAT GTGCTACTCG AATTTCAATG ATATCATTCA CAGCATTATT 
GACATGGACG CCGATGTGAT CACGATTGAG AACTCGCGAT CGGACGAGAA GTTGCTGAGT 
GTGTTCCGTG AGGGTGTGCA CTACGGAGCT GGAATTGGTC CCGGCGTGTA CGATATTCAC 
TCCCCTCGCA TTCCACCAAC AGAGGAGATG GCTGACCGTG CTCGCAAGAT GTTGGCGGTG 
TTGGAGTCCA AGGTATTGTG GATCAACCCT GACTGCGGTC TGAAGACCAG GAAGTATGCC 
GAGGTTGTGC CAGCGTTGAC GAACATGGTG AACGCCGCCA AGCAATTGCG TGCCGAGCTG 
GCGAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024366435.1
CDS	172..2460
Translation	MTSHVVGYPRMGPKRELKFALESMWDGKTQAADLEQVAKDLRASIWKQMSEAGVSMIPSNTFSYYDQVLDTTAMVGAVPARYGWTGGEIGHDVYFSMARGNKTKPAMEMTKWFDTNYHYIVPELSGETKFVYSSRKAVSEYKEAKELGVDTVPVLIGPVTYLLLSKGDKSAPKGFNTLSLLDAILPVYKEVITELKEAGASWIQLDEPKLVMDLEASQYEAFKKAYAVLGEVAQVKLLVETYFTDLPAEAYKTLCDLPIAGFGVDLVRGNKTLSLIKEHGLPEGKTLFAGVVDGRNIWANDLAASVAVVEELQAKLGKDNVVVSTSCSLLHSAVDLKNETKLDSELKSWMAFAAQKLLEVVAVAKAVSGQKDEAFFAANASAQESRRNSPRVHNKAVKEAAAALAGSEHRRSTPVSSRLEQQQKYLNLPILPTTTIGSFPQTPELRRVRREVKSKKISEEDYDKAIKAEIDSVVKLQEELDIDVLVHGEPERNDMVEYFGEQLDGFCFSANGWVQSYGSRCVKPPIIFGDVSRPKAMTVYWSTYAQSVTKRPIKGMLTGPVTILNWSFVRNDQPRSETCYQISLAIKDEVEDLEKANITVIQIDEAALREGLPLRKSEHADYLRWAVHGFRITTCGVKDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVHYGAGIGPGVYDIHSPRIPPTEEMADRARKMLAVLESKVLWINPDCGLKTRKYAEVVPALTNMVNAAKQLRAELAN

Target ORF information:

RefSeq Version	XM_024510667.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase-like (LOC112277871), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024510667.1

ORF Insert Sequence:

ATGACTTCTC ACGTGGTTGG ATATCCCCGC ATGGGCCCCA AGAGGGAGCT CAAGTTTGCT 
CTTGAGTCGA TGTGGGATGG CAAGACCCAG GCAGCTGATT TGGAGCAGGT TGCCAAGGAC 
CTGCGCGCAT CGATCTGGAA GCAGATGTCT GAGGCTGGAG TGAGCATGAT TCCTAGCAAC 
ACATTCTCTT ACTACGACCA GGTGCTGGAC ACTACCGCCA TGGTTGGAGC TGTGCCCGCT 
CGCTACGGAT GGACGGGTGG AGAGATTGGA CACGATGTGT ATTTCTCAAT GGCCCGTGGA 
AACAAGACGA AGCCCGCCAT GGAGATGACC AAGTGGTTCG ATACCAACTA CCACTACATT 
GTGCCAGAGT TGAGCGGTGA GACAAAGTTT GTTTACTCTT CACGCAAGGC CGTGAGCGAG 
TACAAGGAGG CTAAGGAGCT TGGCGTAGAC ACTGTGCCCG TGCTGATAGG TCCAGTGACT 
TATCTGCTGT TGTCCAAGGG TGACAAGAGC GCGCCCAAGG GTTTCAATAC TCTTTCACTT 
CTTGATGCCA TACTTCCCGT GTACAAGGAA GTCATTACAG AGCTGAAGGA AGCTGGTGCG 
TCGTGGATCC AACTGGACGA GCCAAAGTTA GTCATGGACC TTGAAGCTAG CCAATACGAA 
GCTTTTAAGA AGGCATACGC TGTGCTCGGG GAGGTAGCTC AAGTGAAGTT GTTGGTGGAG 
ACATACTTCA CCGACCTGCC TGCCGAGGCC TACAAGACTT TGTGTGACTT GCCAATTGCC 
GGTTTCGGGG TGGACCTCGT CCGCGGCAAC AAAACCTTGA GCCTAATCAA GGAGCACGGT 
CTTCCTGAGG GGAAGACTTT GTTTGCCGGA GTGGTGGACG GAAGGAACAT CTGGGCCAAC 
GACTTGGCTG CCTCTGTGGC CGTGGTTGAG GAATTGCAGG CTAAGCTTGG GAAGGATAAC 
GTAGTTGTCT CAACCTCATG CTCCTTGCTC CATTCCGCAG TGGACCTCAA GAACGAGACA 
AAGTTGGATA GCGAATTGAA GTCCTGGATG GCATTCGCCG CACAGAAGCT GCTGGAGGTA 
GTGGCGGTCG CTAAGGCCGT GTCGGGACAG AAAGACGAGG CTTTCTTCGC GGCTAACGCT 
TCCGCTCAGG AATCGAGGAG GAACTCCCCC CGCGTTCATA ACAAGGCAGT GAAGGAAGCA 
GCCGCTGCTT TGGCCGGTTC GGAGCACCGT CGATCTACCC CGGTATCAAG CCGTCTGGAA 
CAGCAGCAGA AGTACTTGAA CCTGCCAATC CTGCCGACGA CCACGATCGG ATCGTTCCCC 
CAGACGCCAG AGCTCCGCAG GGTCAGGCGT GAGGTGAAGA GCAAGAAGAT CTCAGAGGAG 
GATTATGACA AGGCCATCAA GGCAGAGATT GACAGTGTGG TGAAGCTGCA AGAGGAGCTG 
GACATTGATG TGCTGGTCCA CGGAGAGCCA GAGAGGAATG ATATGGTTGA GTACTTCGGC 
GAGCAGTTGG ATGGTTTCTG CTTCTCAGCC AACGGGTGGG TTCAGTCTTA CGGGTCTCGG 
TGCGTTAAGC CCCCGATTAT CTTCGGTGAC GTGAGCAGGC CGAAGGCCAT GACAGTGTAC 
TGGTCGACTT ACGCTCAGAG CGTGACCAAG AGGCCTATTA AGGGCATGTT GACTGGACCG 
GTTACAATTT TGAACTGGTC TTTCGTGAGG AACGACCAGC CCAGGTCAGA GACCTGTTAC 
CAGATTTCAC TGGCTATCAA GGACGAGGTC GAGGACTTGG AGAAGGCTAA CATTACGGTG 
ATCCAGATCG ATGAGGCTGC CCTTCGCGAG GGACTGCCCC TGCGTAAGTC CGAGCACGCA 
GACTACTTAA GGTGGGCTGT GCACGGGTTC AGAATTACGA CTTGTGGAGT TAAGGATACC 
ACCCAGATTC ACACCCACAT GTGCTACTCG AATTTCAATG ATATCATTCA CAGCATTATT 
GACATGGACG CCGATGTGAT CACGATTGAG AACTCGCGAT CGGACGAGAA GTTGCTGAGT 
GTGTTCCGTG AGGGTGTGCA CTACGGAGCT GGAATTGGTC CCGGCGTGTA CGATATTCAC 
TCCCCTCGCA TTCCACCAAC AGAGGAGATG GCTGACCGTG CTCGCAAGAT GTTGGCGGTG 
TTGGAGTCCA AGGTATTGTG GATCAACCCT GACTGCGGTC TGAAGACCAG GAAGTATGCC 
GAGGTTGTGC CAGCGTTGAC GAACATGGTG AACGCCGCCA AGCAATTGCG TGCCGAGCTG 
GCGAATTAG