LOC112283548 cDNA ORF clone,

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gRNAs

The following LOC112283548 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC112283548 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPn48394	XM_024522373.1 Latest version!	Physcomitrella patens receptor-like kinase LIP2 (LOC112283548), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC112283548 ( XM_024522373.1 ) cDNA ORF clone, -> XP_024378141.1 Physcomitrella patens receptor-like kinase LIP2 (LOC112283548), transcript variant X3, mRNA.

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CloneID	OPn48394
Clone ID Related Accession (Same CDS sequence)	XM_024522373.1
Accession Version	XM_024522373.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	receptor-like kinase LIP2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037258.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGAT TAGAACATAG TACAAGCCGG AAGTGGTCTC GTCGTGAAGA GATGTTAGAG 
GAGGAAGATG AGGAGCTGCC GGTAATGAGT AAGGCTATGG TGGCGAGGCG AAAGTGGTTA 
CAATGGCGGT GGTGTGGGCT GAACGTTTTC GTATTGGTAA TCTTCATGGC TGTGGCGCTT 
GTTACTGAAG TGAGTGCAAG CGAGGGTGTT GAGCAGTTGT CTGCACGAGT GGAGAATTTG 
GGTGAGACTG CAGTACCGAA GGCCATGGCA GGACGTTACA GTACTGTCGA AATTAATTCT 
TGGATCCCCA TATTATCTTT TTGCTTCGTG TTCGTTGTTG GCGTGAGCAC CGGGCTCTGG 
TGGAGTGGTT TCCACATAAT CCCAAGGGCG GAGACAGCTG AACCTTCGTC TTCTTCGCCA 
CCGTCGTCTT CTTCCCTGGG GGAACTGCGC AACCGGCAGA TCGCTCGGGC GAGCTCGGTA 
GGCACGTTGC GGCAGTTTAA GCTGGCTGAG CTAGTGGAGA TGACGGATCA CTTCAAGGAG 
CCGATTGGGC GAGGCGGCCA AGGCACAGTG TACTCTGCTC ATCTGCCTGA GGGCTGCAGC 
AAGGCCGCAG TGAAGAAACT GCAAAAGCAT GAAGCGAATC TTATGCTCCG CGACAATGTG 
GTGAGCGACC ATAACCAAGA GGAAATGGAA AGGGCATTTT GGGAGGAGCT CAAAATAATC 
TCCAAGCTCC ACCATCGCAA CCTTGTGGCT CTTTTAGGCT ACTGCGTCGA AAAGGACGAC 
TTGTTTCTGG TGTATGAATT CATGGGCAAA GGCTCCCTCA GCCAATACCT ACACCCCAAA 
ACCAAGGCAG AAGAGAAGAG CCTGAGCTGG AAGGAGCGTA TGCAATGCGC AGTTGATGTA 
GCGCAGGGTT TGGAATATCT TCACAACCAC GCAAATCCTT CTCTCGTGCA CCGGGACATC 
AAATCTGCCA ACATTCTCTT CGATGATGAA ATGAATGCTA AGATTGCAGA TTTTGGGTTG 
AGTAAGCCTG TTATACTGGA ACAAGAGGCA AGCATGTCGA TTCGACTGCG AGGCACTTAT 
GGATACGTTG ATCCTGTGTA TTTGCTAAAC GGCCGACCCT GTGCCAAGAA CGACGTGTAC 
AGCTACGGCG TGGTACTGTT GGAGCTTATT ACAGGTCGGC GTGCCCTTGA GAACAAAGGC 
AATGTGAACT TGGTACAATG GGTCAAAGAG TCTCTAGGCA CACATGATAT TCCGATGCGT 
CAAATTTCGC GCATTGTTGA CCCCCAGATA GCCCTGGACA TCCTTTACGA CCAGTTCTCT 
GAGGTTGTGA AGCTGGCCCT AAGCTGCGTC CATGAGAGGC AGGATAGCCG GCCCAGCATG 
CAAGATGTGG TTGCTGGATT GTATAATGCC AAGGCCATAG AGGACGCCCA AAGCACCGAG 
TCTCCTCTTG CTCATGTATT GCCTATGAGT CGCAATGGTG GCTCTGTTTA TGATGGTAGT 
TCGATGGGGA CTCCGCGAAC GCACGCGCTG CACGACTGGT CATCATCTTA TGAGATAAAG 
GACAACAGCA TCCTCGTCAA ATGCACCATC GCAGGTTTCA GAATCATGTT GAGATGTAAG 
ATATGGCCAT CCCTCAAGAA CTTTGGGTTG AACAAGGTGG AGGCTTCATT GAACGATGCG 
TTTCTCTGGA AAAAGGGCCT CTGGTTCTAC AATGCAAAGA GCACTCGTGA GTTCACAGTT 
GGTGATGATA TCAAGGTGAT CGCTGAGTGG CATGTCGCAC TAAGAACCGG ATATTACAAT 
CTGCCTGCTA ACGATCCAAG AAGAAGTCAA GGCCCTTTCT GCGTCGAGTA CTTAGCTCTT 
AAGGATGTGA ACGGTCCTTT GGTTGAATAC CTTGGTGACA AGTCACCATG GCCGGAACCC 
GTGCCCCAAG AACGGAGGCC GAGGAGGGAG AGTAGTGGGG GGTCTGGTTC CGGCTCAGTA 
GAACGTCGGA GGAGTAGTGG CCATCACAGG CGTCCCAGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024378141.1
CDS	1309..3330
Translation	MKGLEHSTSRKWSRREEMLEEEDEELPVMSKAMVARRKWLQWRWCGLNVFVLVIFMAVALVTEVSASEGVEQLSARVENLGETAVPKAMAGRYSTVEINSWIPILSFCFVFVVGVSTGLWWSGFHIIPRAETAEPSSSSPPSSSSLGELRNRQIARASSVGTLRQFKLAELVEMTDHFKEPIGRGGQGTVYSAHLPEGCSKAAVKKLQKHEANLMLRDNVVSDHNQEEMERAFWEELKIISKLHHRNLVALLGYCVEKDDLFLVYEFMGKGSLSQYLHPKTKAEEKSLSWKERMQCAVDVAQGLEYLHNHANPSLVHRDIKSANILFDDEMNAKIADFGLSKPVILEQEASMSIRLRGTYGYVDPVYLLNGRPCAKNDVYSYGVVLLELITGRRALENKGNVNLVQWVKESLGTHDIPMRQISRIVDPQIALDILYDQFSEVVKLALSCVHERQDSRPSMQDVVAGLYNAKAIEDAQSTESPLAHVLPMSRNGGSVYDGSSMGTPRTHALHDWSSSYEIKDNSILVKCTIAGFRIMLRCKIWPSLKNFGLNKVEASLNDAFLWKKGLWFYNAKSTREFTVGDDIKVIAEWHVALRTGYYNLPANDPRRSQGPFCVEYLALKDVNGPLVEYLGDKSPWPEPVPQERRPRRESSGGSGSGSVERRRSSGHHRRPS

Target ORF information:

RefSeq Version	XM_024522373.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens receptor-like kinase LIP2 (LOC112283548), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024522373.1

ORF Insert Sequence:

ATGAAGGGAT TAGAACATAG TACAAGCCGG AAGTGGTCTC GTCGTGAAGA GATGTTAGAG 
GAGGAAGATG AGGAGCTGCC GGTAATGAGT AAGGCTATGG TGGCGAGGCG AAAGTGGTTA 
CAATGGCGGT GGTGTGGGCT GAACGTTTTC GTATTGGTAA TCTTCATGGC TGTGGCGCTT 
GTTACTGAAG TGAGTGCAAG CGAGGGTGTT GAGCAGTTGT CTGCACGAGT GGAGAATTTG 
GGTGAGACTG CAGTACCGAA GGCCATGGCA GGACGTTACA GTACTGTCGA AATTAATTCT 
TGGATCCCCA TATTATCTTT TTGCTTCGTG TTCGTTGTTG GCGTGAGCAC CGGGCTCTGG 
TGGAGTGGTT TCCACATAAT CCCAAGGGCG GAGACAGCTG AACCTTCGTC TTCTTCGCCA 
CCGTCGTCTT CTTCCCTGGG GGAACTGCGC AACCGGCAGA TCGCTCGGGC GAGCTCGGTA 
GGCACGTTGC GGCAGTTTAA GCTGGCTGAG CTAGTGGAGA TGACGGATCA CTTCAAGGAG 
CCGATTGGGC GAGGCGGCCA AGGCACAGTG TACTCTGCTC ATCTGCCTGA GGGCTGCAGC 
AAGGCCGCAG TGAAGAAACT GCAAAAGCAT GAAGCGAATC TTATGCTCCG CGACAATGTG 
GTGAGCGACC ATAACCAAGA GGAAATGGAA AGGGCATTTT GGGAGGAGCT CAAAATAATC 
TCCAAGCTCC ACCATCGCAA CCTTGTGGCT CTTTTAGGCT ACTGCGTCGA AAAGGACGAC 
TTGTTTCTGG TGTATGAATT CATGGGCAAA GGCTCCCTCA GCCAATACCT ACACCCCAAA 
ACCAAGGCAG AAGAGAAGAG CCTGAGCTGG AAGGAGCGTA TGCAATGCGC AGTTGATGTA 
GCGCAGGGTT TGGAATATCT TCACAACCAC GCAAATCCTT CTCTCGTGCA CCGGGACATC 
AAATCTGCCA ACATTCTCTT CGATGATGAA ATGAATGCTA AGATTGCAGA TTTTGGGTTG 
AGTAAGCCTG TTATACTGGA ACAAGAGGCA AGCATGTCGA TTCGACTGCG AGGCACTTAT 
GGATACGTTG ATCCTGTGTA TTTGCTAAAC GGCCGACCCT GTGCCAAGAA CGACGTGTAC 
AGCTACGGCG TGGTACTGTT GGAGCTTATT ACAGGTCGGC GTGCCCTTGA GAACAAAGGC 
AATGTGAACT TGGTACAATG GGTCAAAGAG TCTCTAGGCA CACATGATAT TCCGATGCGT 
CAAATTTCGC GCATTGTTGA CCCCCAGATA GCCCTGGACA TCCTTTACGA CCAGTTCTCT 
GAGGTTGTGA AGCTGGCCCT AAGCTGCGTC CATGAGAGGC AGGATAGCCG GCCCAGCATG 
CAAGATGTGG TTGCTGGATT GTATAATGCC AAGGCCATAG AGGACGCCCA AAGCACCGAG 
TCTCCTCTTG CTCATGTATT GCCTATGAGT CGCAATGGTG GCTCTGTTTA TGATGGTAGT 
TCGATGGGGA CTCCGCGAAC GCACGCGCTG CACGACTGGT CATCATCTTA TGAGATAAAG 
GACAACAGCA TCCTCGTCAA ATGCACCATC GCAGGTTTCA GAATCATGTT GAGATGTAAG 
ATATGGCCAT CCCTCAAGAA CTTTGGGTTG AACAAGGTGG AGGCTTCATT GAACGATGCG 
TTTCTCTGGA AAAAGGGCCT CTGGTTCTAC AATGCAAAGA GCACTCGTGA GTTCACAGTT 
GGTGATGATA TCAAGGTGAT CGCTGAGTGG CATGTCGCAC TAAGAACCGG ATATTACAAT 
CTGCCTGCTA ACGATCCAAG AAGAAGTCAA GGCCCTTTCT GCGTCGAGTA CTTAGCTCTT 
AAGGATGTGA ACGGTCCTTT GGTTGAATAC CTTGGTGACA AGTCACCATG GCCGGAACCC 
GTGCCCCAAG AACGGAGGCC GAGGAGGGAG AGTAGTGGGG GGTCTGGTTC CGGCTCAGTA 
GAACGTCGGA GGAGTAGTGG CCATCACAGG CGTCCCAGTT AA