BOH78_2287 cDNA ORF clone, Pichia kudriavzevii(Candida krusei)

Gene
Clones
gRNAs

Summary

Gene Symbol	BOH78_2287
Entrez Gene ID	31692887
Full Name	Puromycin N-acetyltransferase
Gene Type	protein-coding
Organism	Pichia kudriavzevii(Candida krusei)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020688089.1	XP_020544531.1	Puromycin N-acetyltransferase

Pathways from BioSystems
Gene Ontology

The following BOH78_2287 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BOH78_2287 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPi15283	XM_020688089.1 Latest version!	Pichia kudriavzevii Puromycin N-acetyltransferase (BOH78_2287), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BOH78_2287 ( XM_020688089.1 ) cDNA ORF clone, Pichia kudriavzevii(Candida krusei) -> XP_020544531.1 Pichia kudriavzevii Puromycin N-acetyltransferase (BOH78_2287), partial mRNA.

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CloneID	OPi15283
Clone ID Related Accession (Same CDS sequence)	XM_020688089.1
Accession Version	XM_020688089.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-27
Organism	Pichia kudriavzevii(Candida krusei)
Product	Puromycin N-acetyltransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_018150442). COMPLETENESS: incomplete on both ends.

ATGGGAGAGA AGACAAGAAA CGAACCAGTT GAACACGATG AAGAGGCCCA GAGGGAAGAG 
TGCCAGTGGA AGTCCATAGA AACACCAATG CACACCAAGA GAGACCTCGA CAAACCACTT 
AGGTTTGCAT TGCTAGGGCC ACATGGCTCC TCTAAATCGT CATTGGTTTC CATCCTTTCA 
AACAACATGA CTCTTGAGAA CTACTATCCA ACGTTGTACA ACTCACCCAT CTTGGTTGAA 
TTCCAGCCGC CAAACAAGAG AGCAGGAGTT CTACTAGATC CGGTTTCCAC CTTAAATGAT 
CTCTCCAAAG TGGGGGTCAC CAACGACAAG AAGAGTTCAA TTATGTTACC AGAGTACATG 
CTACGCAAGC TGGAAAGGAA GCAGATTGAC AAATTGAAAC GCAAGGATAT AATGGCCCAA 
CCGAACGGCC ACCCGGGTCC AACAACGGTG TCTCCCAAGG AGAGGGAGCC TATTGTTGTG 
GAATTGATCG ACACCCCGGG TGTGGAAGCC GACGACTTGA TTCCATTCTT GGAGAAGTCT 
CTCGACTGCA GACTCTCAAA GGATGTTTTA AACAACTTGG CCAATGGATA CAACACCAAC 
TACCGGTCTC GGGTGAAACC CCTTATCATT GGTTCCGGTA TTAGTGACAT GAATGGATAC 
ATGGACGGAT ATATTCTCAC CTATAGTTGC GTTCCGCAGG ACCCACTTCC GCCAGCCTAC 
GAGGCCTCCG AGAGCACTCC TACAAGGAAG ACAGCCACAC AGTTAGACAT TGTCCGTGCA 
GTCCATGATT CTATTGAGGA TGCATGGAAG GAATGGACCG TTTATAATGA GGGCTGGAAA 
ACCGGTGGGG AAACAGAGGT AAAACATTCC TTCTCGAGCC GCAAGAGGAA AGACAGCACA 
TGCAACCCCC CCACCACTGC TTCAGTGAAC GAATCTCTCA CGGTGGAAGT CCTCTCTATA 
AAGGACTATA AGAAGGCTGC GAGAACATTA CAGATTGCAT TTCAGGACGA CCTGTATGTG 
AATTACCTTA CTTCGGGGCT CTCCGACACA ACCACCAAGG AACAGCTAGA CTTGGCATTG 
TTTGAGGCAA CTGCATATTC GACAATAATG AATGGTCTAC TTGTGGCAGT CCGAGATCGA 
GAAGCCGAAG AAAGTGATCC ACAGGCACCG TTTCTAGCTG TTGCATGTTT CCAGAAACCT 
GAAAAGGACG AGAAATCAAG CACGAGTCGA AGCTTGTTTT CACATCTATG GACTATGTAC 
CAAACCGGGT ATCTAAAATT TGTCTATTTG GCCAATAAAG AAACAAGAAG AAGAGTCTTT 
GATGAGCAAT GGAAACTGCT GGACCAGTTA AAGGAAGAAG TTTTAGGAGA CCAACTTAAT 
AAATCTTGGT ATTTATCCGA TATCGGTGCT ATTCCTAAAG GTCGGGGTAA AGGGCTAGCT 
AGGAAATTAG TTGAATTTGT ATGCCATAAT TACATAGACA GATACATTGT GGAGAAAGAT 
ACAAATGGTG ATGACGATGA CGACGATGAA GAAATCATAA ACAGTGGAGA TGATCGATAT 
ATTGGCTCAA GTGATGAGAA CTCAAGGCTC GACTCGGAGA TAAATTCGTT TCATTTTCAG 
TTTGATTTGG ATTCCGACAA TATCACTGAT TACTCAGGGT ATTCGTCGTT TTCCGATACG 
GAATCTGCAC ATTCGTCTTG GTATAATAAG GAACACAGTA ATAGCAATAC AGATATCGAA 
GACGGTGATG ATGACATATT GTCCAAGTAC GATCGTCAAC AAAGTATGAA GTATCGAAAT 
AGGCGTGGAG CTCCCTTATA TCTAGAATCT TCACACCCGC GTAACAGAAA GATCTACCAG 
AAGTTGGGTT TTACTTATGT GAAGACAGTT CCTGTTGCAG AGGTTCAGTT GGGAAAAGAA 
AATAAAAAGG TATTGACTAT GGATCTAATG GTCAGGGGTG TTAAAGGTAC AAAATGGAAG 
GTAGAAAACG TATTCAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020544531.1
CDS	1..2001
Translation	MGEKTRNEPVEHDEEAQREECQWKSIETPMHTKRDLDKPLRFALLGPHGSSKSSLVSILSNNMTLENYYPTLYNSPILVEFQPPNKRAGVLLDPVSTLNDLSKVGVTNDKKSSIMLPEYMLRKLERKQIDKLKRKDIMAQPNGHPGPTTVSPKEREPIVVELIDTPGVEADDLIPFLEKSLDCRLSKDVLNNLANGYNTNYRSRVKPLIIGSGISDMNGYMDGYILTYSCVPQDPLPPAYEASESTPTRKTATQLDIVRAVHDSIEDAWKEWTVYNEGWKTGGETEVKHSFSSRKRKDSTCNPPTTASVNESLTVEVLSIKDYKKAARTLQIAFQDDLYVNYLTSGLSDTTTKEQLDLALFEATAYSTIMNGLLVAVRDREAEESDPQAPFLAVACFQKPEKDEKSSTSRSLFSHLWTMYQTGYLKFVYLANKETRRRVFDEQWKLLDQLKEEVLGDQLNKSWYLSDIGAIPKGRGKGLARKLVEFVCHNYIDRYIVEKDTNGDDDDDDEEIINSGDDRYIGSSDENSRLDSEINSFHFQFDLDSDNITDYSGYSSFSDTESAHSSWYNKEHSNSNTDIEDGDDDILSKYDRQQSMKYRNRRGAPLYLESSHPRNRKIYQKLGFTYVKTVPVAEVQLGKENKKVLTMDLMVRGVKGTKWKVENVFN

Target ORF information:

RefSeq Version	XM_020688089.1
Organism	Pichia kudriavzevii(Candida krusei)
Definition	Pichia kudriavzevii Puromycin N-acetyltransferase (BOH78_2287), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020688089.1

ORF Insert Sequence:

ATGGGAGAGA AGACAAGAAA CGAACCAGTT GAACACGATG AAGAGGCCCA GAGGGAAGAG 
TGCCAGTGGA AGTCCATAGA AACACCAATG CACACCAAGA GAGACCTCGA CAAACCACTT 
AGGTTTGCAT TGCTAGGGCC ACATGGCTCC TCTAAATCGT CATTGGTTTC CATCCTTTCA 
AACAACATGA CTCTTGAGAA CTACTATCCA ACGTTGTACA ACTCACCCAT CTTGGTTGAA 
TTCCAGCCGC CAAACAAGAG AGCAGGAGTT CTACTAGATC CGGTTTCCAC CTTAAATGAT 
CTCTCCAAAG TGGGGGTCAC CAACGACAAG AAGAGTTCAA TTATGTTACC AGAGTACATG 
CTACGCAAGC TGGAAAGGAA GCAGATTGAC AAATTGAAAC GCAAGGATAT AATGGCCCAA 
CCGAACGGCC ACCCGGGTCC AACAACGGTG TCTCCCAAGG AGAGGGAGCC TATTGTTGTG 
GAATTGATCG ACACCCCGGG TGTGGAAGCC GACGACTTGA TTCCATTCTT GGAGAAGTCT 
CTCGACTGCA GACTCTCAAA GGATGTTTTA AACAACTTGG CCAATGGATA CAACACCAAC 
TACCGGTCTC GGGTGAAACC CCTTATCATT GGTTCCGGTA TTAGTGACAT GAATGGATAC 
ATGGACGGAT ATATTCTCAC CTATAGTTGC GTTCCGCAGG ACCCACTTCC GCCAGCCTAC 
GAGGCCTCCG AGAGCACTCC TACAAGGAAG ACAGCCACAC AGTTAGACAT TGTCCGTGCA 
GTCCATGATT CTATTGAGGA TGCATGGAAG GAATGGACCG TTTATAATGA GGGCTGGAAA 
ACCGGTGGGG AAACAGAGGT AAAACATTCC TTCTCGAGCC GCAAGAGGAA AGACAGCACA 
TGCAACCCCC CCACCACTGC TTCAGTGAAC GAATCTCTCA CGGTGGAAGT CCTCTCTATA 
AAGGACTATA AGAAGGCTGC GAGAACATTA CAGATTGCAT TTCAGGACGA CCTGTATGTG 
AATTACCTTA CTTCGGGGCT CTCCGACACA ACCACCAAGG AACAGCTAGA CTTGGCATTG 
TTTGAGGCAA CTGCATATTC GACAATAATG AATGGTCTAC TTGTGGCAGT CCGAGATCGA 
GAAGCCGAAG AAAGTGATCC ACAGGCACCG TTTCTAGCTG TTGCATGTTT CCAGAAACCT 
GAAAAGGACG AGAAATCAAG CACGAGTCGA AGCTTGTTTT CACATCTATG GACTATGTAC 
CAAACCGGGT ATCTAAAATT TGTCTATTTG GCCAATAAAG AAACAAGAAG AAGAGTCTTT 
GATGAGCAAT GGAAACTGCT GGACCAGTTA AAGGAAGAAG TTTTAGGAGA CCAACTTAAT 
AAATCTTGGT ATTTATCCGA TATCGGTGCT ATTCCTAAAG GTCGGGGTAA AGGGCTAGCT 
AGGAAATTAG TTGAATTTGT ATGCCATAAT TACATAGACA GATACATTGT GGAGAAAGAT 
ACAAATGGTG ATGACGATGA CGACGATGAA GAAATCATAA ACAGTGGAGA TGATCGATAT 
ATTGGCTCAA GTGATGAGAA CTCAAGGCTC GACTCGGAGA TAAATTCGTT TCATTTTCAG 
TTTGATTTGG ATTCCGACAA TATCACTGAT TACTCAGGGT ATTCGTCGTT TTCCGATACG 
GAATCTGCAC ATTCGTCTTG GTATAATAAG GAACACAGTA ATAGCAATAC AGATATCGAA 
GACGGTGATG ATGACATATT GTCCAAGTAC GATCGTCAAC AAAGTATGAA GTATCGAAAT 
AGGCGTGGAG CTCCCTTATA TCTAGAATCT TCACACCCGC GTAACAGAAA GATCTACCAG 
AAGTTGGGTT TTACTTATGT GAAGACAGTT CCTGTTGCAG AGGTTCAGTT GGGAAAAGAA 
AATAAAAAGG TATTGACTAT GGATCTAATG GTCAGGGGTG TTAAAGGTAC AAAATGGAAG 
GTAGAAAACG TATTCAATTG A