FOCAD gene cDNA ORF clone, Pongo abelii(Sumatran orangutan)

mRNA	Protein	Name
XM_009244389.1	XP_009242664.1	focadhesin isoform X1
XM_009244393.1	XP_009242668.1	focadhesin isoform X4
XM_009244395.1	XP_009242670.1	focadhesin isoform X4
XM_024252329.1	XP_024108097.1	focadhesin isoform X3
XM_024252330.1	XP_024108098.1	focadhesin isoform X3
XM_024252328.1	XP_024108096.1	focadhesin isoform X1
XM_009244392.2	XP_009242667.2	focadhesin isoform X4
XM_024252331.1	XP_024108099.1	focadhesin isoform X4
XM_009244390.1	XP_009242665.1	focadhesin isoform X2
XM_009244392.1	XP_009242667.1	focadhesin isoform X4
XM_024252335.1	XP_024108103.1	focadhesin isoform X5
XM_009244391.1	XP_009242666.1	focadhesin isoform X3
XM_024252332.1	XP_024108100.1	focadhesin isoform X4
XM_024252334.1	XP_024108102.1	focadhesin isoform X4
XM_009244394.1	XP_009242669.1	focadhesin isoform X4
XM_009244389.2	XP_009242664.2	focadhesin isoform X2
XM_009244394.2	XP_009242669.2	focadhesin isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa186753	XM_024252328.1 Latest version!	Pongo abelii focadhesin (FOCAD), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa186754	XM_009244389.2 Latest version!	Pongo abelii focadhesin (FOCAD), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa186755	XM_024252329.1 Latest version!	Pongo abelii focadhesin (FOCAD), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa186755	XM_024252330.1 Latest version!	Pongo abelii focadhesin (FOCAD), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa186756	XM_009244392.2 Latest version!	Pongo abelii focadhesin (FOCAD), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa186756	XM_009244394.2 Latest version!	Pongo abelii focadhesin (FOCAD), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa186756	XM_024252332.1 Latest version!	Pongo abelii focadhesin (FOCAD), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa186756	XM_024252331.1 Latest version!	Pongo abelii focadhesin (FOCAD), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa186756	XM_024252334.1 Latest version!	Pongo abelii focadhesin (FOCAD), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa186757	XM_024252335.1 Latest version!	Pongo abelii focadhesin (FOCAD), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa07887	XM_009244395.1 Latest version!	Pongo abelii focadhesin (FOCAD), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OSa07887	XM_009244394.1 Latest version!	Pongo abelii focadhesin (FOCAD), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OSa07887	XM_009244393.1 Latest version!	Pongo abelii focadhesin (FOCAD), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OSa07887	XM_009244392.1 Latest version!	Pongo abelii focadhesin (FOCAD), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OSa28571	XM_009244389.1 Latest version!	Pongo abelii focadhesin (FOCAD), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OSa28572	XM_009244390.1 Latest version!	Pongo abelii focadhesin (FOCAD), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OSa28573	XM_009244391.1 Latest version!	Pongo abelii focadhesin (FOCAD), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

FOCAD ( XM_024252328.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024108096.1 Pongo abelii focadhesin (FOCAD), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa186753
Clone ID Related Accession (Same CDS sequence)	XM_024252328.1
Accession Version	XM_024252328.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	focadhesin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036912.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAATA ATAACTATAT TTGGATGAAG TGGACCAATG AACCATCCAC TGAAGCTCCT 
AAACTGGAGA TTTATGCTTT CGAAGACAAT CAAGGAAATT TTCATCCACA CAGATTAGCA 
GTTGAAAATG AAGCTGCACA CATACATGTA AGAGAAGCAA AAATGTCAGA TGATATCAGG 
AAAAGGTTTG AATTTCCAAA TTCTCTTATC CAATCACAGG CTGCGGGTCA TCTTATTGCT 
GCAGTCCTAA AGGAGAATGG TTTTTCAGAA AAGATTTACC AATCTACAAA TCAGACTCCT 
GCTTTGAACT TGCTGTGGGA GAAGTGTTGC AGTGACAATG TAGTGGTTCG AACAGCCTGC 
TGTGAAGGTC TGGTGGCACT CGTTGCTCAG GATCATGCAG AGTTCAGCTA TGTTCTCAAT 
GGGATACTCA ACTTGATTCC ATCAACCAGA AATACACATG GCTTGATAAA AGCCATTATG 
AAATTACTAC AAATGCAAGC TCTTAAGGAA GGACAAGGTG GGGAAAAGAA TATTCAGAGT 
GTATATACCA TTAGAAATCA TCCTCATCCT TTGATCACTG TGCTTGAACA CAGACCTGAT 
TGTTGGCCAG TGTTTTTGCA GCAGCTGACA GCATTTTTCC AGCAGTGCCC TGAAAGGTCA 
GAAGTTTCAT GCATTCAAAT AATGGCACCA TTTCTGTGGT ATCTGTATTG TGAACCATCT 
CAGTTACAAG AATATGCTAA ACTCCGACTA GCCCTGCTGA AAGTCTTACT TCAACCCCAG 
GTTCTTTGTG ACAAGGATCA ACCATCAATA CTGCAACAGC AGATACTTCA ACTGTGTTGT 
GACATAGTTC CATGTTTGCA GATAAAAGAT TTGATACAGA CAACAGAAGC GATGATGTTT 
ATTGAGGAAA TATATTTAAG CCTTTTGCGT CATCCTGTTT TCTGGAAAAT TCAGCTTACC 
CAGATGACTC TTCAGCTGCT GTGTGTCGGT GAAGTCAGCT TAAAGATAAC TGGTGAATGT 
TCATCTTCAA TTCACCTCTT AGAGCACAGT GTTGAACTTC TGAAGGAGGA TTTTCCTGTT 
GAACTGGTCA TAATTGGAAT AGCTTTACTA CTTCTACAGA CTCCAGCAAG TCAGCAGAAG 
CCAATCTTAA ATCTAGCTTT GAAGCTCTTC TCTGTTACTG AGGATCAGAA AATCCCAAAG 
TCCTCTCTGC TGCTAGTGAT GCCAATTCTG CAGATACTGT CTTCTACTGC CTTGGAAGAC 
TGTATATCTG TGGATGAAGA AGGTCCCTCT AGGCAGCAGT TGGCTCTAAA CCTTTTGGAA 
ATGATACAGC AGGAATGTTA CAGAGATGAC CACCAAAAGC TCTCCTACAA GCTTGTGTGC 
CCTGTAACCA GTATGTATGG TTCAGTATTT ACAGCCTGGA GGATTCTTGA AGTAATGACA 
GACTCCTCTG CTGCAAGTGA CTGGTTGGCT TCAGTAGAGT CATTGCTTCC TATTACTACT 
GTGATCCCTG TGCCTGCCTT TCTTCTGCTG GCTCACCTCC TTGTTGAAGA CAAAGGACAA 
AATCTTCACC AAATACTCAA GGTCACTACA GAATTAGCCC AAGCAGATTC CTCCCAGGTG 
CCAAATCTGA TTCCAGTTTT GATGTTCAAA TTGGGAAGAC CACTGGAACC TATATTATAT 
AATGATATAT TGTATACTTT ACCTAAGCTT GGTGTTCACA AGGTGTGTAT AGGACAAATT 
CTACGAGTAA TACAACTGCT TGGAACCACA CCACGACTAA GAGCTGTCAC TTTGCGCTTG 
CTGACATCTT TGTGGGAAAA GCAGGACCGA GTCTATCCTG AACTGCAGCG TTTCATGGCT 
GTGTCTGATG TACCTTCTCT TTCGGTGGGC AAGGAAGTCC AATGGGAGAA ACTGATTGCA 
AAAGCAGCAT CAATCAGAGA TATATGTAAG CAGAGGCCAT ATCAACATGG TGCAGATATG 
CTGGCAGCTA TTTCTCAGGT GTTGAATGAA TGCACCAAGC CTGATCAAGC TACTCCAGCA 
GCCTTGGTAT TACAAGGTCT TCATGCACTC TGTCAAGCTG AGGTTGTTTG CATTCGCTCC 
ACTTGGAATG CTCTCTCTCC AAAGCTGAGT TGTGACACAA GACCTCTCAT TCTGAAGACA 
CTGAGTGAAC TATTTTCTCT AGTTCCTTCC TTAACAGTCA ATACAACTGA ATATGAGAAT 
TTTAAAGTTC AAGTCCTCAG CTTCCTCTGG ACTCATACTC AAAACAAGGA CCCAATTGTG 
GCAAATGCTG CATATAGATC CCTGGCCAAT TTTAGTGCAG GAGAACACAC CATTCTTCAT 
CTGCCTGAAA AGATAAGACC AGAAATTCCC ATTCCTGAAG AGTTAGATGA TGATGAAGAT 
GATGAGGATG TGGATCTTTC AGTTCCTGGC TCTTGCTATC TCAAACTGTT GTCACTCACT 
CCCCCTTTGG TTTTACCAGC TTTGGAGGAA TTTTTTACAT CACTTGTGAA GCAAGAAATG 
GTGAATATGC CTCGTGGGAT ATATCACTCT GCATTAAAAG GAGGTGTCCG CTCAGACCAA 
GGAAAGACTG TAGCAGGAAT CCCCAATTTT ATATTGAAAA TGTATGAAAC AAACAAGCAA 
CCAGGATTGA AACCTGGCCT TGCAGGTGGT ATGTTATTTT GCTATGATGT TTCCATGTAT 
CAGAGTAAAG ATGGAAAACC ATTGAATAGA CTGATGGCCA GCAGAGGGCG AAGTTTCAAG 
CAGACATCAC TTGCTCTTGT ACATGAGGTT CATATCCAGC TTTCAGAGTG GCACCGTGCG 
ATTTTTCTTC CACAGGCCTG GCTTGCATAC ATGAATCGAG CTTATCATGC CATTTTACAG 
GGAAGACTAG GAGAGCTGGA GTTGCAGTTA AAACATGGAA AAGAAGAACC TGAGGAGGTG 
CAGTACAAAA AAAGCACAGC TTGGCTCTGG GTTAGAGACA TGCTGACTGA TGAGATCACC 
AAGGCAGCTG CAAAGGAGAG TCCGGTAGTG AAAGGCAATG CGCTGTTAGC TCTAAGCAGC 
CTTGCTGTCG TCGTATCTAG ACATGAAGCC AGCGTCTCCT CAGACTCTGA CGGGCTCCTG 
GAGGTTCAAC CTAATTTCCT TTCAATGAAA GAGTGGGTTT CCATGATACT TGATACACTC 
TTGGTCATTG TGGATAGCCA TTACCAACCC AGAGGGCAAC TTCTCTCCTG GTTTTATTAT 
AAGTCCTATT CTGGTGAAAA CACAGCTAGT GCCATTGCCC GTTCTGCTGC CGCCACGGCT 
TTGTCTCTCC TTGTGCCAGT TTTCATTATC TCTTGCAAAG AGAAGGTTGA GGAAATCCTG 
AACATGCTGA CTGCCAGGTT ACCTGGGAAA CCAAGTGCTG ATGAGTCTCA AGCCGTGCAA 
ATCCACATGG GCCTTGCTTT AGGGATGTTT CTCTCTCGCT TGTGTGAAGA GAAACTCAGT 
GATATATGTG GCCAAGAGAT GAACCTTCTT CTGATGAAGT CGTTGGATGC CCTGGAAAAT 
TGCTGCTTTG ACACTAGTCT TGAATACAAC ACGGGCTGTA TATTGGGAGT TGGACTTGTT 
CTGTCCCTCA TGAGTCACAG CAGCCAAATG CAGTCCTGCG TTCACGTGAC AGCATTGCTT 
CGGAAGCTGT CTGCATACCT AGATGACAGT GGGAGCCAGA GCAGAACGTT TCAGGAGGTC 
CTTGCCTACA CACTTAGCTG TGTATGTACA TCAGCGTTCA GTGCTGGAAT TATTGAGGCT 
ACAGAGGCTG AGGATGTTAT GAACAAGCTT CGACTGTTAG TGGAGAATAG CCAGCAGACT 
TCAGGTTTTG CCCTGGCTTT AGGAAACGTA GTTCATGGAT TGTCTGTGTG TGGACATGGA 
AAAGCTGAAG ACTTGGGCAG CAAACTACTC CCTGCCTGGA TCAGAATTGT TCTAACAGAG 
GGCACTCCCA CAATGCTTTG TCTGGCAGCT CTTCATGGCA TGGTGGCCCT GGTAGGCTCT 
GAAGGGGATG TAATGCAGCT GAAATCAGAA GCCATCCAGA CCTCTCATTT TCAAGGCAGA 
CTTAATGAAG TCATTAGAAC CTTAACTCAG GTCATTAGTG TCTCTGGGGT GATTGGTCTC 
CAGTCAAATG CAGTCTGGCT TCTTGGACAT CTTCATCTAT CTACTCTATC CTCAAGTCAA 
AGTAGAGCCT CTGTTCCTAC TGACTATAGC TATTTGCCTG AAAGCAGTTT TATTGGAGCA 
GCTATTGGCT TCTTCATTAC AGGAGGAAAA AAAGGTCCTG AATCTGTGCC TCCTTCCCTT 
CTTAAAGTAG TGATGAAACC CATAGCAACT GTTGGAGAAA GCTACCAATA TCCTCCTGTG 
AACTGGGCTG CACTTCTCTC TCCACTTATG AGGCTAAATT TTGGTGAAGA GATCCAGCAA 
CTGTGCCTTG AAATTATGGT GACCCAGGCA CAGTCATCCC AGAATGCAGC TGCACTATTG 
GGCTTGTGGG TGACACCACC ACTGATCCAC AGTCTGAGTC TGAATACCAA GAGATATCTC 
CTGATATCTG CACCTCTGTG GATAAAACAC ATCTCTGATG AACAGATCCT GGGTTTTGTT 
GAAAATTTAA TGGTGGCAGT TTTTAAAGCA GCTTCCCCAC TTGGAAATCC TGAGCTATGC 
CCGAGTGCTT TACAGGGTCT GAGCCAGGCC ATGAAACTGC CCAGCCCTGC TCACCACCTC 
TGGAGTCTGC TCTCTGAAGC TACTGGGAAA ATTTTTGACC TCCTGCCAAA TAAGATTCGG 
AGAAAGGATC TAGAGCTGTA TATCAGCATA GCAAAATGCC TCTTAGAAAT GACAGATGAT 
GATGCCAATC GGATCGCCCA GGTTACTAAG AGCAACATAG AAAAGGCTGC CTTTGTCAAA 
CTGTACTTAG TCTCTCAAGG ACGATTCCCC TTGGTGAACC TGACCGATAT GCTGAGTGTT 
GCTGTGCAGC ACCGTGAGAA AGAGGTGTTG GCCTGGATGA TTCTGCACAG CTTATACCAG 
GCACGGATTG TGAGCCATGC CAACACGGGC GTTTTGAAGA GAATGGAGTG GCTCTTGGAA 
CTGATGGGTT ATATTAGAAA TGTTGCGTAC CAGTCAACAT CCTTTCAGAA TACGGCTCTT 
GATGAGGCTT TGGACTTCTT CTTGCTGATA TTTGCAACCG CAGTGGTTGC ATGGGCCGAC 
CACACTGCCC CTCTCCTCCT TGGTCTCAGT GCCAGTTGGT TGCCATGGCA TCAGGAGAAT 
GGCCCGGCTG GGCCAGTACC AAGCTTCCTT GGCAGGAGTC CAATGCACAG GGTCACTGTG 
CAGGAGGTTC TCACTCTCCT TCCCAATAGC ATGGCTCTGC TGCTGCAGAA AGAGCCATGG 
AAGGAAGAGA CCCAGAAGTT CATTGACTGG CTATTCAGCA TCATGGAAAG CCCTAAAGAA 
GCCCTCTCAG CAAAGTCCAG GGATCTTTTG AAAGCCACCC TGCTGTCCTT GAGAGTTCTC 
CCAGAGTTTA AGAAGAAAGC TGTATGGACC AGAGCGTATG GTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024108096.1
CDS	46..5613
Translation	MKNNNYIWMKWTNEPSTEAPKLEIYAFEDNQGNFHPHRLAVENEAAHIHVREAKMSDDIRKRFEFPNSLIQSQAAGHLIAAVLKENGFSEKIYQSTNQTPALNLLWEKCCSDNVVVRTACCEGLVALVAQDHAEFSYVLNGILNLIPSTRNTHGLIKAIMKLLQMQALKEGQGGEKNIQSVYTIRNHPHPLITVLEHRPDCWPVFLQQLTAFFQQCPERSEVSCIQIMAPFLWYLYCEPSQLQEYAKLRLALLKVLLQPQVLCDKDQPSILQQQILQLCCDIVPCLQIKDLIQTTEAMMFIEEIYLSLLRHPVFWKIQLTQMTLQLLCVGEVSLKITGECSSSIHLLEHSVELLKEDFPVELVIIGIALLLLQTPASQQKPILNLALKLFSVTEDQKIPKSSLLLVMPILQILSSTALEDCISVDEEGPSRQQLALNLLEMIQQECYRDDHQKLSYKLVCPVTSMYGSVFTAWRILEVMTDSSAASDWLASVESLLPITTVIPVPAFLLLAHLLVEDKGQNLHQILKVTTELAQADSSQVPNLIPVLMFKLGRPLEPILYNDILYTLPKLGVHKVCIGQILRVIQLLGTTPRLRAVTLRLLTSLWEKQDRVYPELQRFMAVSDVPSLSVGKEVQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQATPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDTRPLILKTLSELFSLVPSLTVNTTEYENFKVQVLSFLWTHTQNKDPIVANAAYRSLANFSAGEHTILHLPEKIRPEIPIPEELDDDEDDEDVDLSVPGSCYLKLLSLTPPLVLPALEEFFTSLVKQEMVNMPRGIYHSALKGGVRSDQGKTVAGIPNFILKMYETNKQPGLKPGLAGGMLFCYDVSMYQSKDGKPLNRLMASRGRSFKQTSLALVHEVHIQLSEWHRAIFLPQAWLAYMNRAYHAILQGRLGELELQLKHGKEEPEEVQYKKSTAWLWVRDMLTDEITKAAAKESPVVKGNALLALSSLAVVVSRHEASVSSDSDGLLEVQPNFLSMKEWVSMILDTLLVIVDSHYQPRGQLLSWFYYKSYSGENTASAIARSAAATALSLLVPVFIISCKEKVEEILNMLTARLPGKPSADESQAVQIHMGLALGMFLSRLCEEKLSDICGQEMNLLLMKSLDALENCCFDTSLEYNTGCILGVGLVLSLMSHSSQMQSCVHVTALLRKLSAYLDDSGSQSRTFQEVLAYTLSCVCTSAFSAGIIEATEAEDVMNKLRLLVENSQQTSGFALALGNVVHGLSVCGHGKAEDLGSKLLPAWIRIVLTEGTPTMLCLAALHGMVALVGSEGDVMQLKSEAIQTSHFQGRLNEVIRTLTQVISVSGVIGLQSNAVWLLGHLHLSTLSSSQSRASVPTDYSYLPESSFIGAAIGFFITGGKKGPESVPPSLLKVVMKPIATVGESYQYPPVNWAALLSPLMRLNFGEEIQQLCLEIMVTQAQSSQNAAALLGLWVTPPLIHSLSLNTKRYLLISAPLWIKHISDEQILGFVENLMVAVFKAASPLGNPELCPSALQGLSQAMKLPSPAHHLWSLLSEATGKIFDLLPNKIRRKDLELYISIAKCLLEMTDDDANRIAQVTKSNIEKAAFVKLYLVSQGRFPLVNLTDMLSVAVQHREKEVLAWMILHSLYQARIVSHANTGVLKRMEWLLELMGYIRNVAYQSTSFQNTALDEALDFFLLIFATAVVAWADHTAPLLLGLSASWLPWHQENGPAGPVPSFLGRSPMHRVTVQEVLTLLPNSMALLLQKEPWKEETQKFIDWLFSIMESPKEALSAKSRDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_024252328.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii focadhesin (FOCAD), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024252328.1

ORF Insert Sequence:

ATGAAAAATA ATAACTATAT TTGGATGAAG TGGACCAATG AACCATCCAC TGAAGCTCCT 
AAACTGGAGA TTTATGCTTT CGAAGACAAT CAAGGAAATT TTCATCCACA CAGATTAGCA 
GTTGAAAATG AAGCTGCACA CATACATGTA AGAGAAGCAA AAATGTCAGA TGATATCAGG 
AAAAGGTTTG AATTTCCAAA TTCTCTTATC CAATCACAGG CTGCGGGTCA TCTTATTGCT 
GCAGTCCTAA AGGAGAATGG TTTTTCAGAA AAGATTTACC AATCTACAAA TCAGACTCCT 
GCTTTGAACT TGCTGTGGGA GAAGTGTTGC AGTGACAATG TAGTGGTTCG AACAGCCTGC 
TGTGAAGGTC TGGTGGCACT CGTTGCTCAG GATCATGCAG AGTTCAGCTA TGTTCTCAAT 
GGGATACTCA ACTTGATTCC ATCAACCAGA AATACACATG GCTTGATAAA AGCCATTATG 
AAATTACTAC AAATGCAAGC TCTTAAGGAA GGACAAGGTG GGGAAAAGAA TATTCAGAGT 
GTATATACCA TTAGAAATCA TCCTCATCCT TTGATCACTG TGCTTGAACA CAGACCTGAT 
TGTTGGCCAG TGTTTTTGCA GCAGCTGACA GCATTTTTCC AGCAGTGCCC TGAAAGGTCA 
GAAGTTTCAT GCATTCAAAT AATGGCACCA TTTCTGTGGT ATCTGTATTG TGAACCATCT 
CAGTTACAAG AATATGCTAA ACTCCGACTA GCCCTGCTGA AAGTCTTACT TCAACCCCAG 
GTTCTTTGTG ACAAGGATCA ACCATCAATA CTGCAACAGC AGATACTTCA ACTGTGTTGT 
GACATAGTTC CATGTTTGCA GATAAAAGAT TTGATACAGA CAACAGAAGC GATGATGTTT 
ATTGAGGAAA TATATTTAAG CCTTTTGCGT CATCCTGTTT TCTGGAAAAT TCAGCTTACC 
CAGATGACTC TTCAGCTGCT GTGTGTCGGT GAAGTCAGCT TAAAGATAAC TGGTGAATGT 
TCATCTTCAA TTCACCTCTT AGAGCACAGT GTTGAACTTC TGAAGGAGGA TTTTCCTGTT 
GAACTGGTCA TAATTGGAAT AGCTTTACTA CTTCTACAGA CTCCAGCAAG TCAGCAGAAG 
CCAATCTTAA ATCTAGCTTT GAAGCTCTTC TCTGTTACTG AGGATCAGAA AATCCCAAAG 
TCCTCTCTGC TGCTAGTGAT GCCAATTCTG CAGATACTGT CTTCTACTGC CTTGGAAGAC 
TGTATATCTG TGGATGAAGA AGGTCCCTCT AGGCAGCAGT TGGCTCTAAA CCTTTTGGAA 
ATGATACAGC AGGAATGTTA CAGAGATGAC CACCAAAAGC TCTCCTACAA GCTTGTGTGC 
CCTGTAACCA GTATGTATGG TTCAGTATTT ACAGCCTGGA GGATTCTTGA AGTAATGACA 
GACTCCTCTG CTGCAAGTGA CTGGTTGGCT TCAGTAGAGT CATTGCTTCC TATTACTACT 
GTGATCCCTG TGCCTGCCTT TCTTCTGCTG GCTCACCTCC TTGTTGAAGA CAAAGGACAA 
AATCTTCACC AAATACTCAA GGTCACTACA GAATTAGCCC AAGCAGATTC CTCCCAGGTG 
CCAAATCTGA TTCCAGTTTT GATGTTCAAA TTGGGAAGAC CACTGGAACC TATATTATAT 
AATGATATAT TGTATACTTT ACCTAAGCTT GGTGTTCACA AGGTGTGTAT AGGACAAATT 
CTACGAGTAA TACAACTGCT TGGAACCACA CCACGACTAA GAGCTGTCAC TTTGCGCTTG 
CTGACATCTT TGTGGGAAAA GCAGGACCGA GTCTATCCTG AACTGCAGCG TTTCATGGCT 
GTGTCTGATG TACCTTCTCT TTCGGTGGGC AAGGAAGTCC AATGGGAGAA ACTGATTGCA 
AAAGCAGCAT CAATCAGAGA TATATGTAAG CAGAGGCCAT ATCAACATGG TGCAGATATG 
CTGGCAGCTA TTTCTCAGGT GTTGAATGAA TGCACCAAGC CTGATCAAGC TACTCCAGCA 
GCCTTGGTAT TACAAGGTCT TCATGCACTC TGTCAAGCTG AGGTTGTTTG CATTCGCTCC 
ACTTGGAATG CTCTCTCTCC AAAGCTGAGT TGTGACACAA GACCTCTCAT TCTGAAGACA 
CTGAGTGAAC TATTTTCTCT AGTTCCTTCC TTAACAGTCA ATACAACTGA ATATGAGAAT 
TTTAAAGTTC AAGTCCTCAG CTTCCTCTGG ACTCATACTC AAAACAAGGA CCCAATTGTG 
GCAAATGCTG CATATAGATC CCTGGCCAAT TTTAGTGCAG GAGAACACAC CATTCTTCAT 
CTGCCTGAAA AGATAAGACC AGAAATTCCC ATTCCTGAAG AGTTAGATGA TGATGAAGAT 
GATGAGGATG TGGATCTTTC AGTTCCTGGC TCTTGCTATC TCAAACTGTT GTCACTCACT 
CCCCCTTTGG TTTTACCAGC TTTGGAGGAA TTTTTTACAT CACTTGTGAA GCAAGAAATG 
GTGAATATGC CTCGTGGGAT ATATCACTCT GCATTAAAAG GAGGTGTCCG CTCAGACCAA 
GGAAAGACTG TAGCAGGAAT CCCCAATTTT ATATTGAAAA TGTATGAAAC AAACAAGCAA 
CCAGGATTGA AACCTGGCCT TGCAGGTGGT ATGTTATTTT GCTATGATGT TTCCATGTAT 
CAGAGTAAAG ATGGAAAACC ATTGAATAGA CTGATGGCCA GCAGAGGGCG AAGTTTCAAG 
CAGACATCAC TTGCTCTTGT ACATGAGGTT CATATCCAGC TTTCAGAGTG GCACCGTGCG 
ATTTTTCTTC CACAGGCCTG GCTTGCATAC ATGAATCGAG CTTATCATGC CATTTTACAG 
GGAAGACTAG GAGAGCTGGA GTTGCAGTTA AAACATGGAA AAGAAGAACC TGAGGAGGTG 
CAGTACAAAA AAAGCACAGC TTGGCTCTGG GTTAGAGACA TGCTGACTGA TGAGATCACC 
AAGGCAGCTG CAAAGGAGAG TCCGGTAGTG AAAGGCAATG CGCTGTTAGC TCTAAGCAGC 
CTTGCTGTCG TCGTATCTAG ACATGAAGCC AGCGTCTCCT CAGACTCTGA CGGGCTCCTG 
GAGGTTCAAC CTAATTTCCT TTCAATGAAA GAGTGGGTTT CCATGATACT TGATACACTC 
TTGGTCATTG TGGATAGCCA TTACCAACCC AGAGGGCAAC TTCTCTCCTG GTTTTATTAT 
AAGTCCTATT CTGGTGAAAA CACAGCTAGT GCCATTGCCC GTTCTGCTGC CGCCACGGCT 
TTGTCTCTCC TTGTGCCAGT TTTCATTATC TCTTGCAAAG AGAAGGTTGA GGAAATCCTG 
AACATGCTGA CTGCCAGGTT ACCTGGGAAA CCAAGTGCTG ATGAGTCTCA AGCCGTGCAA 
ATCCACATGG GCCTTGCTTT AGGGATGTTT CTCTCTCGCT TGTGTGAAGA GAAACTCAGT 
GATATATGTG GCCAAGAGAT GAACCTTCTT CTGATGAAGT CGTTGGATGC CCTGGAAAAT 
TGCTGCTTTG ACACTAGTCT TGAATACAAC ACGGGCTGTA TATTGGGAGT TGGACTTGTT 
CTGTCCCTCA TGAGTCACAG CAGCCAAATG CAGTCCTGCG TTCACGTGAC AGCATTGCTT 
CGGAAGCTGT CTGCATACCT AGATGACAGT GGGAGCCAGA GCAGAACGTT TCAGGAGGTC 
CTTGCCTACA CACTTAGCTG TGTATGTACA TCAGCGTTCA GTGCTGGAAT TATTGAGGCT 
ACAGAGGCTG AGGATGTTAT GAACAAGCTT CGACTGTTAG TGGAGAATAG CCAGCAGACT 
TCAGGTTTTG CCCTGGCTTT AGGAAACGTA GTTCATGGAT TGTCTGTGTG TGGACATGGA 
AAAGCTGAAG ACTTGGGCAG CAAACTACTC CCTGCCTGGA TCAGAATTGT TCTAACAGAG 
GGCACTCCCA CAATGCTTTG TCTGGCAGCT CTTCATGGCA TGGTGGCCCT GGTAGGCTCT 
GAAGGGGATG TAATGCAGCT GAAATCAGAA GCCATCCAGA CCTCTCATTT TCAAGGCAGA 
CTTAATGAAG TCATTAGAAC CTTAACTCAG GTCATTAGTG TCTCTGGGGT GATTGGTCTC 
CAGTCAAATG CAGTCTGGCT TCTTGGACAT CTTCATCTAT CTACTCTATC CTCAAGTCAA 
AGTAGAGCCT CTGTTCCTAC TGACTATAGC TATTTGCCTG AAAGCAGTTT TATTGGAGCA 
GCTATTGGCT TCTTCATTAC AGGAGGAAAA AAAGGTCCTG AATCTGTGCC TCCTTCCCTT 
CTTAAAGTAG TGATGAAACC CATAGCAACT GTTGGAGAAA GCTACCAATA TCCTCCTGTG 
AACTGGGCTG CACTTCTCTC TCCACTTATG AGGCTAAATT TTGGTGAAGA GATCCAGCAA 
CTGTGCCTTG AAATTATGGT GACCCAGGCA CAGTCATCCC AGAATGCAGC TGCACTATTG 
GGCTTGTGGG TGACACCACC ACTGATCCAC AGTCTGAGTC TGAATACCAA GAGATATCTC 
CTGATATCTG CACCTCTGTG GATAAAACAC ATCTCTGATG AACAGATCCT GGGTTTTGTT 
GAAAATTTAA TGGTGGCAGT TTTTAAAGCA GCTTCCCCAC TTGGAAATCC TGAGCTATGC 
CCGAGTGCTT TACAGGGTCT GAGCCAGGCC ATGAAACTGC CCAGCCCTGC TCACCACCTC 
TGGAGTCTGC TCTCTGAAGC TACTGGGAAA ATTTTTGACC TCCTGCCAAA TAAGATTCGG 
AGAAAGGATC TAGAGCTGTA TATCAGCATA GCAAAATGCC TCTTAGAAAT GACAGATGAT 
GATGCCAATC GGATCGCCCA GGTTACTAAG AGCAACATAG AAAAGGCTGC CTTTGTCAAA 
CTGTACTTAG TCTCTCAAGG ACGATTCCCC TTGGTGAACC TGACCGATAT GCTGAGTGTT 
GCTGTGCAGC ACCGTGAGAA AGAGGTGTTG GCCTGGATGA TTCTGCACAG CTTATACCAG 
GCACGGATTG TGAGCCATGC CAACACGGGC GTTTTGAAGA GAATGGAGTG GCTCTTGGAA 
CTGATGGGTT ATATTAGAAA TGTTGCGTAC CAGTCAACAT CCTTTCAGAA TACGGCTCTT 
GATGAGGCTT TGGACTTCTT CTTGCTGATA TTTGCAACCG CAGTGGTTGC ATGGGCCGAC 
CACACTGCCC CTCTCCTCCT TGGTCTCAGT GCCAGTTGGT TGCCATGGCA TCAGGAGAAT 
GGCCCGGCTG GGCCAGTACC AAGCTTCCTT GGCAGGAGTC CAATGCACAG GGTCACTGTG 
CAGGAGGTTC TCACTCTCCT TCCCAATAGC ATGGCTCTGC TGCTGCAGAA AGAGCCATGG 
AAGGAAGAGA CCCAGAAGTT CATTGACTGG CTATTCAGCA TCATGGAAAG CCCTAAAGAA 
GCCCTCTCAG CAAAGTCCAG GGATCTTTTG AAAGCCACCC TGCTGTCCTT GAGAGTTCTC 
CCAGAGTTTA AGAAGAAAGC TGTATGGACC AGAGCGTATG GTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FOCAD ( XM_009244389.2 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009242664.2 Pongo abelii focadhesin (FOCAD), transcript variant X2, mRNA.

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CloneID	OSa186754
Clone ID Related Accession (Same CDS sequence)	XM_009244389.2
Accession Version	XM_009244389.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	focadhesin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036912.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 28, 2018 this sequence version replaced XM_009244389.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAATA ATAACTATAT TTGGATGAAG TGGACCAATG AACCATCCAC TGAAGCTCCT 
AAACTGGAGA TTTATGCTTT CGAAGAAGCT GCACACATAC ATGTAAGAGA AGCAAAAATG 
TCAGATGATA TCAGGAAAAG GTTTGAATTT CCAAATTCTC TTATCCAATC ACAGGCTGCG 
GGTCATCTTA TTGCTGCAGT CCTAAAGGAG AATGGTTTTT CAGAAAAGAT TTACCAATCT 
ACAAATCAGA CTCCTGCTTT GAACTTGCTG TGGGAGAAGT GTTGCAGTGA CAATGTAGTG 
GTTCGAACAG CCTGCTGTGA AGGTCTGGTG GCACTCGTTG CTCAGGATCA TGCAGAGTTC 
AGCTATGTTC TCAATGGGAT ACTCAACTTG ATTCCATCAA CCAGAAATAC ACATGGCTTG 
ATAAAAGCCA TTATGAAATT ACTACAAATG CAAGCTCTTA AGGAAGGACA AGGTGGGGAA 
AAGAATATTC AGAGTGTATA TACCATTAGA AATCATCCTC ATCCTTTGAT CACTGTGCTT 
GAACACAGAC CTGATTGTTG GCCAGTGTTT TTGCAGCAGC TGACAGCATT TTTCCAGCAG 
TGCCCTGAAA GGTCAGAAGT TTCATGCATT CAAATAATGG CACCATTTCT GTGGTATCTG 
TATTGTGAAC CATCTCAGTT ACAAGAATAT GCTAAACTCC GACTAGCCCT GCTGAAAGTC 
TTACTTCAAC CCCAGGTTCT TTGTGACAAG GATCAACCAT CAATACTGCA ACAGCAGATA 
CTTCAACTGT GTTGTGACAT AGTTCCATGT TTGCAGATAA AAGATTTGAT ACAGACAACA 
GAAGCGATGA TGTTTATTGA GGAAATATAT TTAAGCCTTT TGCGTCATCC TGTTTTCTGG 
AAAATTCAGC TTACCCAGAT GACTCTTCAG CTGCTGTGTG TCGGTGAAGT CAGCTTAAAG 
ATAACTGGTG AATGTTCATC TTCAATTCAC CTCTTAGAGC ACAGTGTTGA ACTTCTGAAG 
GAGGATTTTC CTGTTGAACT GGTCATAATT GGAATAGCTT TACTACTTCT ACAGACTCCA 
GCAAGTCAGC AGAAGCCAAT CTTAAATCTA GCTTTGAAGC TCTTCTCTGT TACTGAGGAT 
CAGAAAATCC CAAAGTCCTC TCTGCTGCTA GTGATGCCAA TTCTGCAGAT ACTGTCTTCT 
ACTGCCTTGG AAGACTGTAT ATCTGTGGAT GAAGAAGGTC CCTCTAGGCA GCAGTTGGCT 
CTAAACCTTT TGGAAATGAT ACAGCAGGAA TGTTACAGAG ATGACCACCA AAAGCTCTCC 
TACAAGCTTG TGTGCCCTGT AACCAGTATG TATGGTTCAG TATTTACAGC CTGGAGGATT 
CTTGAAGTAA TGACAGACTC CTCTGCTGCA AGTGACTGGT TGGCTTCAGT AGAGTCATTG 
CTTCCTATTA CTACTGTGAT CCCTGTGCCT GCCTTTCTTC TGCTGGCTCA CCTCCTTGTT 
GAAGACAAAG GACAAAATCT TCACCAAATA CTCAAGGTCA CTACAGAATT AGCCCAAGCA 
GATTCCTCCC AGGTGCCAAA TCTGATTCCA GTTTTGATGT TCAAATTGGG AAGACCACTG 
GAACCTATAT TATATAATGA TATATTGTAT ACTTTACCTA AGCTTGGTGT TCACAAGGTG 
TGTATAGGAC AAATTCTACG AGTAATACAA CTGCTTGGAA CCACACCACG ACTAAGAGCT 
GTCACTTTGC GCTTGCTGAC ATCTTTGTGG GAAAAGCAGG ACCGAGTCTA TCCTGAACTG 
CAGCGTTTCA TGGCTGTGTC TGATGTACCT TCTCTTTCGG TGGGCAAGGA AGTCCAATGG 
GAGAAACTGA TTGCAAAAGC AGCATCAATC AGAGATATAT GTAAGCAGAG GCCATATCAA 
CATGGTGCAG ATATGCTGGC AGCTATTTCT CAGGTGTTGA ATGAATGCAC CAAGCCTGAT 
CAAGCTACTC CAGCAGCCTT GGTATTACAA GGTCTTCATG CACTCTGTCA AGCTGAGGTT 
GTTTGCATTC GCTCCACTTG GAATGCTCTC TCTCCAAAGC TGAGTTGTGA CACAAGACCT 
CTCATTCTGA AGACACTGAG TGAACTATTT TCTCTAGTTC CTTCCTTAAC AGTCAATACA 
ACTGAATATG AGAATTTTAA AGTTCAAGTC CTCAGCTTCC TCTGGACTCA TACTCAAAAC 
AAGGACCCAA TTGTGGCAAA TGCTGCATAT AGATCCCTGG CCAATTTTAG TGCAGGAGAA 
CACACCATTC TTCATCTGCC TGAAAAGATA AGACCAGAAA TTCCCATTCC TGAAGAGTTA 
GATGATGATG AAGATGATGA GGATGTGGAT CTTTCAGTTC CTGGCTCTTG CTATCTCAAA 
CTGTTGTCAC TCACTCCCCC TTTGGTTTTA CCAGCTTTGG AGGAATTTTT TACATCACTT 
GTGAAGCAAG AAATGGTGAA TATGCCTCGT GGGATATATC ACTCTGCATT AAAAGGAGGT 
GTCCGCTCAG ACCAAGGAAA GACTGTAGCA GGAATCCCCA ATTTTATATT GAAAATGTAT 
GAAACAAACA AGCAACCAGG ATTGAAACCT GGCCTTGCAG GTGGTATGTT ATTTTGCTAT 
GATGTTTCCA TGTATCAGAG TAAAGATGGA AAACCATTGA ATAGACTGAT GGCCAGCAGA 
GGGCGAAGTT TCAAGCAGAC ATCACTTGCT CTTGTACATG AGGTTCATAT CCAGCTTTCA 
GAGTGGCACC GTGCGATTTT TCTTCCACAG GCCTGGCTTG CATACATGAA TCGAGCTTAT 
CATGCCATTT TACAGGGAAG ACTAGGAGAG CTGGAGTTGC AGTTAAAACA TGGAAAAGAA 
GAACCTGAGG AGGTGCAGTA CAAAAAAAGC ACAGCTTGGC TCTGGGTTAG AGACATGCTG 
ACTGATGAGA TCACCAAGGC AGCTGCAAAG GAGAGTCCGG TAGTGAAAGG CAATGCGCTG 
TTAGCTCTAA GCAGCCTTGC TGTCGTCGTA TCTAGACATG AAGCCAGCGT CTCCTCAGAC 
TCTGACGGGC TCCTGGAGGT TCAACCTAAT TTCCTTTCAA TGAAAGAGTG GGTTTCCATG 
ATACTTGATA CACTCTTGGT CATTGTGGAT AGCCATTACC AACCCAGAGG GCAACTTCTC 
TCCTGGTTTT ATTATAAGTC CTATTCTGGT GAAAACACAG CTAGTGCCAT TGCCCGTTCT 
GCTGCCGCCA CGGCTTTGTC TCTCCTTGTG CCAGTTTTCA TTATCTCTTG CAAAGAGAAG 
GTTGAGGAAA TCCTGAACAT GCTGACTGCC AGGTTACCTG GGAAACCAAG TGCTGATGAG 
TCTCAAGCCG TGCAAATCCA CATGGGCCTT GCTTTAGGGA TGTTTCTCTC TCGCTTGTGT 
GAAGAGAAAC TCAGTGATAT ATGTGGCCAA GAGATGAACC TTCTTCTGAT GAAGTCGTTG 
GATGCCCTGG AAAATTGCTG CTTTGACACT AGTCTTGAAT ACAACACGGG CTGTATATTG 
GGAGTTGGAC TTGTTCTGTC CCTCATGAGT CACAGCAGCC AAATGCAGTC CTGCGTTCAC 
GTGACAGCAT TGCTTCGGAA GCTGTCTGCA TACCTAGATG ACAGTGGGAG CCAGAGCAGA 
ACGTTTCAGG AGGTCCTTGC CTACACACTT AGCTGTGTAT GTACATCAGC GTTCAGTGCT 
GGAATTATTG AGGCTACAGA GGCTGAGGAT GTTATGAACA AGCTTCGACT GTTAGTGGAG 
AATAGCCAGC AGACTTCAGG TTTTGCCCTG GCTTTAGGAA ACGTAGTTCA TGGATTGTCT 
GTGTGTGGAC ATGGAAAAGC TGAAGACTTG GGCAGCAAAC TACTCCCTGC CTGGATCAGA 
ATTGTTCTAA CAGAGGGCAC TCCCACAATG CTTTGTCTGG CAGCTCTTCA TGGCATGGTG 
GCCCTGGTAG GCTCTGAAGG GGATGTAATG CAGCTGAAAT CAGAAGCCAT CCAGACCTCT 
CATTTTCAAG GCAGACTTAA TGAAGTCATT AGAACCTTAA CTCAGGTCAT TAGTGTCTCT 
GGGGTGATTG GTCTCCAGTC AAATGCAGTC TGGCTTCTTG GACATCTTCA TCTATCTACT 
CTATCCTCAA GTCAAAGTAG AGCCTCTGTT CCTACTGACT ATAGCTATTT GCCTGAAAGC 
AGTTTTATTG GAGCAGCTAT TGGCTTCTTC ATTACAGGAG GAAAAAAAGG TCCTGAATCT 
GTGCCTCCTT CCCTTCTTAA AGTAGTGATG AAACCCATAG CAACTGTTGG AGAAAGCTAC 
CAATATCCTC CTGTGAACTG GGCTGCACTT CTCTCTCCAC TTATGAGGCT AAATTTTGGT 
GAAGAGATCC AGCAACTGTG CCTTGAAATT ATGGTGACCC AGGCACAGTC ATCCCAGAAT 
GCAGCTGCAC TATTGGGCTT GTGGGTGACA CCACCACTGA TCCACAGTCT GAGTCTGAAT 
ACCAAGAGAT ATCTCCTGAT ATCTGCACCT CTGTGGATAA AACACATCTC TGATGAACAG 
ATCCTGGGTT TTGTTGAAAA TTTAATGGTG GCAGTTTTTA AAGCAGCTTC CCCACTTGGA 
AATCCTGAGC TATGCCCGAG TGCTTTACAG GGTCTGAGCC AGGCCATGAA ACTGCCCAGC 
CCTGCTCACC ACCTCTGGAG TCTGCTCTCT GAAGCTACTG GGAAAATTTT TGACCTCCTG 
CCAAATAAGA TTCGGAGAAA GGATCTAGAG CTGTATATCA GCATAGCAAA ATGCCTCTTA 
GAAATGACAG ATGATGATGC CAATCGGATC GCCCAGGTTA CTAAGAGCAA CATAGAAAAG 
GCTGCCTTTG TCAAACTGTA CTTAGTCTCT CAAGGACGAT TCCCCTTGGT GAACCTGACC 
GATATGCTGA GTGTTGCTGT GCAGCACCGT GAGAAAGAGG TGTTGGCCTG GATGATTCTG 
CACAGCTTAT ACCAGGCACG GATTGTGAGC CATGCCAACA CGGGCGTTTT GAAGAGAATG 
GAGTGGCTCT TGGAACTGAT GGGTTATATT AGAAATGTTG CGTACCAGTC AACATCCTTT 
CAGAATACGG CTCTTGATGA GGCTTTGGAC TTCTTCTTGC TGATATTTGC AACCGCAGTG 
GTTGCATGGG CCGACCACAC TGCCCCTCTC CTCCTTGGTC TCAGTGCCAG TTGGTTGCCA 
TGGCATCAGG AGAATGGCCC GGCTGGGCCA GTACCAAGCT TCCTTGGCAG GAGTCCAATG 
CACAGGGTCA CTGTGCAGGA GGTTCTCACT CTCCTTCCCA ATAGCATGGC TCTGCTGCTG 
CAGAAAGAGC CATGGAAGGA AGAGACCCAG AAGTTCATTG ACTGGCTATT CAGCATCATG 
GAAAGCCCTA AAGAAGCCCT CTCAGCAAAG TCCAGGGATC TTTTGAAAGC CACCCTGCTG 
TCCTTGAGAG TTCTCCCAGA GTTTAAGAAG AAAGCTGTAT GGACCAGAGC GTATGGTTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009242664.2
CDS	46..5568
Translation	MKNNNYIWMKWTNEPSTEAPKLEIYAFEEAAHIHVREAKMSDDIRKRFEFPNSLIQSQAAGHLIAAVLKENGFSEKIYQSTNQTPALNLLWEKCCSDNVVVRTACCEGLVALVAQDHAEFSYVLNGILNLIPSTRNTHGLIKAIMKLLQMQALKEGQGGEKNIQSVYTIRNHPHPLITVLEHRPDCWPVFLQQLTAFFQQCPERSEVSCIQIMAPFLWYLYCEPSQLQEYAKLRLALLKVLLQPQVLCDKDQPSILQQQILQLCCDIVPCLQIKDLIQTTEAMMFIEEIYLSLLRHPVFWKIQLTQMTLQLLCVGEVSLKITGECSSSIHLLEHSVELLKEDFPVELVIIGIALLLLQTPASQQKPILNLALKLFSVTEDQKIPKSSLLLVMPILQILSSTALEDCISVDEEGPSRQQLALNLLEMIQQECYRDDHQKLSYKLVCPVTSMYGSVFTAWRILEVMTDSSAASDWLASVESLLPITTVIPVPAFLLLAHLLVEDKGQNLHQILKVTTELAQADSSQVPNLIPVLMFKLGRPLEPILYNDILYTLPKLGVHKVCIGQILRVIQLLGTTPRLRAVTLRLLTSLWEKQDRVYPELQRFMAVSDVPSLSVGKEVQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQATPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDTRPLILKTLSELFSLVPSLTVNTTEYENFKVQVLSFLWTHTQNKDPIVANAAYRSLANFSAGEHTILHLPEKIRPEIPIPEELDDDEDDEDVDLSVPGSCYLKLLSLTPPLVLPALEEFFTSLVKQEMVNMPRGIYHSALKGGVRSDQGKTVAGIPNFILKMYETNKQPGLKPGLAGGMLFCYDVSMYQSKDGKPLNRLMASRGRSFKQTSLALVHEVHIQLSEWHRAIFLPQAWLAYMNRAYHAILQGRLGELELQLKHGKEEPEEVQYKKSTAWLWVRDMLTDEITKAAAKESPVVKGNALLALSSLAVVVSRHEASVSSDSDGLLEVQPNFLSMKEWVSMILDTLLVIVDSHYQPRGQLLSWFYYKSYSGENTASAIARSAAATALSLLVPVFIISCKEKVEEILNMLTARLPGKPSADESQAVQIHMGLALGMFLSRLCEEKLSDICGQEMNLLLMKSLDALENCCFDTSLEYNTGCILGVGLVLSLMSHSSQMQSCVHVTALLRKLSAYLDDSGSQSRTFQEVLAYTLSCVCTSAFSAGIIEATEAEDVMNKLRLLVENSQQTSGFALALGNVVHGLSVCGHGKAEDLGSKLLPAWIRIVLTEGTPTMLCLAALHGMVALVGSEGDVMQLKSEAIQTSHFQGRLNEVIRTLTQVISVSGVIGLQSNAVWLLGHLHLSTLSSSQSRASVPTDYSYLPESSFIGAAIGFFITGGKKGPESVPPSLLKVVMKPIATVGESYQYPPVNWAALLSPLMRLNFGEEIQQLCLEIMVTQAQSSQNAAALLGLWVTPPLIHSLSLNTKRYLLISAPLWIKHISDEQILGFVENLMVAVFKAASPLGNPELCPSALQGLSQAMKLPSPAHHLWSLLSEATGKIFDLLPNKIRRKDLELYISIAKCLLEMTDDDANRIAQVTKSNIEKAAFVKLYLVSQGRFPLVNLTDMLSVAVQHREKEVLAWMILHSLYQARIVSHANTGVLKRMEWLLELMGYIRNVAYQSTSFQNTALDEALDFFLLIFATAVVAWADHTAPLLLGLSASWLPWHQENGPAGPVPSFLGRSPMHRVTVQEVLTLLPNSMALLLQKEPWKEETQKFIDWLFSIMESPKEALSAKSRDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_009244389.2
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii focadhesin (FOCAD), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009244389.2

ORF Insert Sequence:

ATGAAAAATA ATAACTATAT TTGGATGAAG TGGACCAATG AACCATCCAC TGAAGCTCCT 
AAACTGGAGA TTTATGCTTT CGAAGAAGCT GCACACATAC ATGTAAGAGA AGCAAAAATG 
TCAGATGATA TCAGGAAAAG GTTTGAATTT CCAAATTCTC TTATCCAATC ACAGGCTGCG 
GGTCATCTTA TTGCTGCAGT CCTAAAGGAG AATGGTTTTT CAGAAAAGAT TTACCAATCT 
ACAAATCAGA CTCCTGCTTT GAACTTGCTG TGGGAGAAGT GTTGCAGTGA CAATGTAGTG 
GTTCGAACAG CCTGCTGTGA AGGTCTGGTG GCACTCGTTG CTCAGGATCA TGCAGAGTTC 
AGCTATGTTC TCAATGGGAT ACTCAACTTG ATTCCATCAA CCAGAAATAC ACATGGCTTG 
ATAAAAGCCA TTATGAAATT ACTACAAATG CAAGCTCTTA AGGAAGGACA AGGTGGGGAA 
AAGAATATTC AGAGTGTATA TACCATTAGA AATCATCCTC ATCCTTTGAT CACTGTGCTT 
GAACACAGAC CTGATTGTTG GCCAGTGTTT TTGCAGCAGC TGACAGCATT TTTCCAGCAG 
TGCCCTGAAA GGTCAGAAGT TTCATGCATT CAAATAATGG CACCATTTCT GTGGTATCTG 
TATTGTGAAC CATCTCAGTT ACAAGAATAT GCTAAACTCC GACTAGCCCT GCTGAAAGTC 
TTACTTCAAC CCCAGGTTCT TTGTGACAAG GATCAACCAT CAATACTGCA ACAGCAGATA 
CTTCAACTGT GTTGTGACAT AGTTCCATGT TTGCAGATAA AAGATTTGAT ACAGACAACA 
GAAGCGATGA TGTTTATTGA GGAAATATAT TTAAGCCTTT TGCGTCATCC TGTTTTCTGG 
AAAATTCAGC TTACCCAGAT GACTCTTCAG CTGCTGTGTG TCGGTGAAGT CAGCTTAAAG 
ATAACTGGTG AATGTTCATC TTCAATTCAC CTCTTAGAGC ACAGTGTTGA ACTTCTGAAG 
GAGGATTTTC CTGTTGAACT GGTCATAATT GGAATAGCTT TACTACTTCT ACAGACTCCA 
GCAAGTCAGC AGAAGCCAAT CTTAAATCTA GCTTTGAAGC TCTTCTCTGT TACTGAGGAT 
CAGAAAATCC CAAAGTCCTC TCTGCTGCTA GTGATGCCAA TTCTGCAGAT ACTGTCTTCT 
ACTGCCTTGG AAGACTGTAT ATCTGTGGAT GAAGAAGGTC CCTCTAGGCA GCAGTTGGCT 
CTAAACCTTT TGGAAATGAT ACAGCAGGAA TGTTACAGAG ATGACCACCA AAAGCTCTCC 
TACAAGCTTG TGTGCCCTGT AACCAGTATG TATGGTTCAG TATTTACAGC CTGGAGGATT 
CTTGAAGTAA TGACAGACTC CTCTGCTGCA AGTGACTGGT TGGCTTCAGT AGAGTCATTG 
CTTCCTATTA CTACTGTGAT CCCTGTGCCT GCCTTTCTTC TGCTGGCTCA CCTCCTTGTT 
GAAGACAAAG GACAAAATCT TCACCAAATA CTCAAGGTCA CTACAGAATT AGCCCAAGCA 
GATTCCTCCC AGGTGCCAAA TCTGATTCCA GTTTTGATGT TCAAATTGGG AAGACCACTG 
GAACCTATAT TATATAATGA TATATTGTAT ACTTTACCTA AGCTTGGTGT TCACAAGGTG 
TGTATAGGAC AAATTCTACG AGTAATACAA CTGCTTGGAA CCACACCACG ACTAAGAGCT 
GTCACTTTGC GCTTGCTGAC ATCTTTGTGG GAAAAGCAGG ACCGAGTCTA TCCTGAACTG 
CAGCGTTTCA TGGCTGTGTC TGATGTACCT TCTCTTTCGG TGGGCAAGGA AGTCCAATGG 
GAGAAACTGA TTGCAAAAGC AGCATCAATC AGAGATATAT GTAAGCAGAG GCCATATCAA 
CATGGTGCAG ATATGCTGGC AGCTATTTCT CAGGTGTTGA ATGAATGCAC CAAGCCTGAT 
CAAGCTACTC CAGCAGCCTT GGTATTACAA GGTCTTCATG CACTCTGTCA AGCTGAGGTT 
GTTTGCATTC GCTCCACTTG GAATGCTCTC TCTCCAAAGC TGAGTTGTGA CACAAGACCT 
CTCATTCTGA AGACACTGAG TGAACTATTT TCTCTAGTTC CTTCCTTAAC AGTCAATACA 
ACTGAATATG AGAATTTTAA AGTTCAAGTC CTCAGCTTCC TCTGGACTCA TACTCAAAAC 
AAGGACCCAA TTGTGGCAAA TGCTGCATAT AGATCCCTGG CCAATTTTAG TGCAGGAGAA 
CACACCATTC TTCATCTGCC TGAAAAGATA AGACCAGAAA TTCCCATTCC TGAAGAGTTA 
GATGATGATG AAGATGATGA GGATGTGGAT CTTTCAGTTC CTGGCTCTTG CTATCTCAAA 
CTGTTGTCAC TCACTCCCCC TTTGGTTTTA CCAGCTTTGG AGGAATTTTT TACATCACTT 
GTGAAGCAAG AAATGGTGAA TATGCCTCGT GGGATATATC ACTCTGCATT AAAAGGAGGT 
GTCCGCTCAG ACCAAGGAAA GACTGTAGCA GGAATCCCCA ATTTTATATT GAAAATGTAT 
GAAACAAACA AGCAACCAGG ATTGAAACCT GGCCTTGCAG GTGGTATGTT ATTTTGCTAT 
GATGTTTCCA TGTATCAGAG TAAAGATGGA AAACCATTGA ATAGACTGAT GGCCAGCAGA 
GGGCGAAGTT TCAAGCAGAC ATCACTTGCT CTTGTACATG AGGTTCATAT CCAGCTTTCA 
GAGTGGCACC GTGCGATTTT TCTTCCACAG GCCTGGCTTG CATACATGAA TCGAGCTTAT 
CATGCCATTT TACAGGGAAG ACTAGGAGAG CTGGAGTTGC AGTTAAAACA TGGAAAAGAA 
GAACCTGAGG AGGTGCAGTA CAAAAAAAGC ACAGCTTGGC TCTGGGTTAG AGACATGCTG 
ACTGATGAGA TCACCAAGGC AGCTGCAAAG GAGAGTCCGG TAGTGAAAGG CAATGCGCTG 
TTAGCTCTAA GCAGCCTTGC TGTCGTCGTA TCTAGACATG AAGCCAGCGT CTCCTCAGAC 
TCTGACGGGC TCCTGGAGGT TCAACCTAAT TTCCTTTCAA TGAAAGAGTG GGTTTCCATG 
ATACTTGATA CACTCTTGGT CATTGTGGAT AGCCATTACC AACCCAGAGG GCAACTTCTC 
TCCTGGTTTT ATTATAAGTC CTATTCTGGT GAAAACACAG CTAGTGCCAT TGCCCGTTCT 
GCTGCCGCCA CGGCTTTGTC TCTCCTTGTG CCAGTTTTCA TTATCTCTTG CAAAGAGAAG 
GTTGAGGAAA TCCTGAACAT GCTGACTGCC AGGTTACCTG GGAAACCAAG TGCTGATGAG 
TCTCAAGCCG TGCAAATCCA CATGGGCCTT GCTTTAGGGA TGTTTCTCTC TCGCTTGTGT 
GAAGAGAAAC TCAGTGATAT ATGTGGCCAA GAGATGAACC TTCTTCTGAT GAAGTCGTTG 
GATGCCCTGG AAAATTGCTG CTTTGACACT AGTCTTGAAT ACAACACGGG CTGTATATTG 
GGAGTTGGAC TTGTTCTGTC CCTCATGAGT CACAGCAGCC AAATGCAGTC CTGCGTTCAC 
GTGACAGCAT TGCTTCGGAA GCTGTCTGCA TACCTAGATG ACAGTGGGAG CCAGAGCAGA 
ACGTTTCAGG AGGTCCTTGC CTACACACTT AGCTGTGTAT GTACATCAGC GTTCAGTGCT 
GGAATTATTG AGGCTACAGA GGCTGAGGAT GTTATGAACA AGCTTCGACT GTTAGTGGAG 
AATAGCCAGC AGACTTCAGG TTTTGCCCTG GCTTTAGGAA ACGTAGTTCA TGGATTGTCT 
GTGTGTGGAC ATGGAAAAGC TGAAGACTTG GGCAGCAAAC TACTCCCTGC CTGGATCAGA 
ATTGTTCTAA CAGAGGGCAC TCCCACAATG CTTTGTCTGG CAGCTCTTCA TGGCATGGTG 
GCCCTGGTAG GCTCTGAAGG GGATGTAATG CAGCTGAAAT CAGAAGCCAT CCAGACCTCT 
CATTTTCAAG GCAGACTTAA TGAAGTCATT AGAACCTTAA CTCAGGTCAT TAGTGTCTCT 
GGGGTGATTG GTCTCCAGTC AAATGCAGTC TGGCTTCTTG GACATCTTCA TCTATCTACT 
CTATCCTCAA GTCAAAGTAG AGCCTCTGTT CCTACTGACT ATAGCTATTT GCCTGAAAGC 
AGTTTTATTG GAGCAGCTAT TGGCTTCTTC ATTACAGGAG GAAAAAAAGG TCCTGAATCT 
GTGCCTCCTT CCCTTCTTAA AGTAGTGATG AAACCCATAG CAACTGTTGG AGAAAGCTAC 
CAATATCCTC CTGTGAACTG GGCTGCACTT CTCTCTCCAC TTATGAGGCT AAATTTTGGT 
GAAGAGATCC AGCAACTGTG CCTTGAAATT ATGGTGACCC AGGCACAGTC ATCCCAGAAT 
GCAGCTGCAC TATTGGGCTT GTGGGTGACA CCACCACTGA TCCACAGTCT GAGTCTGAAT 
ACCAAGAGAT ATCTCCTGAT ATCTGCACCT CTGTGGATAA AACACATCTC TGATGAACAG 
ATCCTGGGTT TTGTTGAAAA TTTAATGGTG GCAGTTTTTA AAGCAGCTTC CCCACTTGGA 
AATCCTGAGC TATGCCCGAG TGCTTTACAG GGTCTGAGCC AGGCCATGAA ACTGCCCAGC 
CCTGCTCACC ACCTCTGGAG TCTGCTCTCT GAAGCTACTG GGAAAATTTT TGACCTCCTG 
CCAAATAAGA TTCGGAGAAA GGATCTAGAG CTGTATATCA GCATAGCAAA ATGCCTCTTA 
GAAATGACAG ATGATGATGC CAATCGGATC GCCCAGGTTA CTAAGAGCAA CATAGAAAAG 
GCTGCCTTTG TCAAACTGTA CTTAGTCTCT CAAGGACGAT TCCCCTTGGT GAACCTGACC 
GATATGCTGA GTGTTGCTGT GCAGCACCGT GAGAAAGAGG TGTTGGCCTG GATGATTCTG 
CACAGCTTAT ACCAGGCACG GATTGTGAGC CATGCCAACA CGGGCGTTTT GAAGAGAATG 
GAGTGGCTCT TGGAACTGAT GGGTTATATT AGAAATGTTG CGTACCAGTC AACATCCTTT 
CAGAATACGG CTCTTGATGA GGCTTTGGAC TTCTTCTTGC TGATATTTGC AACCGCAGTG 
GTTGCATGGG CCGACCACAC TGCCCCTCTC CTCCTTGGTC TCAGTGCCAG TTGGTTGCCA 
TGGCATCAGG AGAATGGCCC GGCTGGGCCA GTACCAAGCT TCCTTGGCAG GAGTCCAATG 
CACAGGGTCA CTGTGCAGGA GGTTCTCACT CTCCTTCCCA ATAGCATGGC TCTGCTGCTG 
CAGAAAGAGC CATGGAAGGA AGAGACCCAG AAGTTCATTG ACTGGCTATT CAGCATCATG 
GAAAGCCCTA AAGAAGCCCT CTCAGCAAAG TCCAGGGATC TTTTGAAAGC CACCCTGCTG 
TCCTTGAGAG TTCTCCCAGA GTTTAAGAAG AAAGCTGTAT GGACCAGAGC GTATGGTTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FOCAD ( XM_024252329.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024108097.1 Pongo abelii focadhesin (FOCAD), transcript variant X3, mRNA.

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CloneID	OSa186755
Clone ID Related Accession (Same CDS sequence)	XM_024252329.1 , XM_024252330.1
Accession Version	XM_024252329.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	focadhesin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036912.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAACA AAGGAAGGCA AGAAGCTGCA CACATACATG TAAGAGAAGC AAAAATGTCA 
GATGATATCA GGAAAAGGTT TGAATTTCCA AATTCTCTTA TCCAATCACA GGCTGCGGGT 
CATCTTATTG CTGCAGTCCT AAAGGAGAAT GGTTTTTCAG AAAAGATTTA CCAATCTACA 
AATCAGACTC CTGCTTTGAA CTTGCTGTGG GAGAAGTGTT GCAGTGACAA TGTAGTGGTT 
CGAACAGCCT GCTGTGAAGG TCTGGTGGCA CTCGTTGCTC AGGATCATGC AGAGTTCAGC 
TATGTTCTCA ATGGGATACT CAACTTGATT CCATCAACCA GAAATACACA TGGCTTGATA 
AAAGCCATTA TGAAATTACT ACAAATGCAA GCTCTTAAGG AAGGACAAGG TGGGGAAAAG 
AATATTCAGA GTGTATATAC CATTAGAAAT CATCCTCATC CTTTGATCAC TGTGCTTGAA 
CACAGACCTG ATTGTTGGCC AGTGTTTTTG CAGCAGCTGA CAGCATTTTT CCAGCAGTGC 
CCTGAAAGGT CAGAAGTTTC ATGCATTCAA ATAATGGCAC CATTTCTGTG GTATCTGTAT 
TGTGAACCAT CTCAGTTACA AGAATATGCT AAACTCCGAC TAGCCCTGCT GAAAGTCTTA 
CTTCAACCCC AGGTTCTTTG TGACAAGGAT CAACCATCAA TACTGCAACA GCAGATACTT 
CAACTGTGTT GTGACATAGT TCCATGTTTG CAGATAAAAG ATTTGATACA GACAACAGAA 
GCGATGATGT TTATTGAGGA AATATATTTA AGCCTTTTGC GTCATCCTGT TTTCTGGAAA 
ATTCAGCTTA CCCAGATGAC TCTTCAGCTG CTGTGTGTCG GTGAAGTCAG CTTAAAGATA 
ACTGGTGAAT GTTCATCTTC AATTCACCTC TTAGAGCACA GTGTTGAACT TCTGAAGGAG 
GATTTTCCTG TTGAACTGGT CATAATTGGA ATAGCTTTAC TACTTCTACA GACTCCAGCA 
AGTCAGCAGA AGCCAATCTT AAATCTAGCT TTGAAGCTCT TCTCTGTTAC TGAGGATCAG 
AAAATCCCAA AGTCCTCTCT GCTGCTAGTG ATGCCAATTC TGCAGATACT GTCTTCTACT 
GCCTTGGAAG ACTGTATATC TGTGGATGAA GAAGGTCCCT CTAGGCAGCA GTTGGCTCTA 
AACCTTTTGG AAATGATACA GCAGGAATGT TACAGAGATG ACCACCAAAA GCTCTCCTAC 
AAGCTTGTGT GCCCTGTAAC CAGTATGTAT GGTTCAGTAT TTACAGCCTG GAGGATTCTT 
GAAGTAATGA CAGACTCCTC TGCTGCAAGT GACTGGTTGG CTTCAGTAGA GTCATTGCTT 
CCTATTACTA CTGTGATCCC TGTGCCTGCC TTTCTTCTGC TGGCTCACCT CCTTGTTGAA 
GACAAAGGAC AAAATCTTCA CCAAATACTC AAGGTCACTA CAGAATTAGC CCAAGCAGAT 
TCCTCCCAGG TGCCAAATCT GATTCCAGTT TTGATGTTCA AATTGGGAAG ACCACTGGAA 
CCTATATTAT ATAATGATAT ATTGTATACT TTACCTAAGC TTGGTGTTCA CAAGGTGTGT 
ATAGGACAAA TTCTACGAGT AATACAACTG CTTGGAACCA CACCACGACT AAGAGCTGTC 
ACTTTGCGCT TGCTGACATC TTTGTGGGAA AAGCAGGACC GAGTCTATCC TGAACTGCAG 
CGTTTCATGG CTGTGTCTGA TGTACCTTCT CTTTCGGTGG GCAAGGAAGT CCAATGGGAG 
AAACTGATTG CAAAAGCAGC ATCAATCAGA GATATATGTA AGCAGAGGCC ATATCAACAT 
GGTGCAGATA TGCTGGCAGC TATTTCTCAG GTGTTGAATG AATGCACCAA GCCTGATCAA 
GCTACTCCAG CAGCCTTGGT ATTACAAGGT CTTCATGCAC TCTGTCAAGC TGAGGTTGTT 
TGCATTCGCT CCACTTGGAA TGCTCTCTCT CCAAAGCTGA GTTGTGACAC AAGACCTCTC 
ATTCTGAAGA CACTGAGTGA ACTATTTTCT CTAGTTCCTT CCTTAACAGT CAATACAACT 
GAATATGAGA ATTTTAAAGT TCAAGTCCTC AGCTTCCTCT GGACTCATAC TCAAAACAAG 
GACCCAATTG TGGCAAATGC TGCATATAGA TCCCTGGCCA ATTTTAGTGC AGGAGAACAC 
ACCATTCTTC ATCTGCCTGA AAAGATAAGA CCAGAAATTC CCATTCCTGA AGAGTTAGAT 
GATGATGAAG ATGATGAGGA TGTGGATCTT TCAGTTCCTG GCTCTTGCTA TCTCAAACTG 
TTGTCACTCA CTCCCCCTTT GGTTTTACCA GCTTTGGAGG AATTTTTTAC ATCACTTGTG 
AAGCAAGAAA TGGTGAATAT GCCTCGTGGG ATATATCACT CTGCATTAAA AGGAGGTGTC 
CGCTCAGACC AAGGAAAGAC TGTAGCAGGA ATCCCCAATT TTATATTGAA AATGTATGAA 
ACAAACAAGC AACCAGGATT GAAACCTGGC CTTGCAGGTG GTATGTTATT TTGCTATGAT 
GTTTCCATGT ATCAGAGTAA AGATGGAAAA CCATTGAATA GACTGATGGC CAGCAGAGGG 
CGAAGTTTCA AGCAGACATC ACTTGCTCTT GTACATGAGG TTCATATCCA GCTTTCAGAG 
TGGCACCGTG CGATTTTTCT TCCACAGGCC TGGCTTGCAT ACATGAATCG AGCTTATCAT 
GCCATTTTAC AGGGAAGACT AGGAGAGCTG GAGTTGCAGT TAAAACATGG AAAAGAAGAA 
CCTGAGGAGG TGCAGTACAA AAAAAGCACA GCTTGGCTCT GGGTTAGAGA CATGCTGACT 
GATGAGATCA CCAAGGCAGC TGCAAAGGAG AGTCCGGTAG TGAAAGGCAA TGCGCTGTTA 
GCTCTAAGCA GCCTTGCTGT CGTCGTATCT AGACATGAAG CCAGCGTCTC CTCAGACTCT 
GACGGGCTCC TGGAGGTTCA ACCTAATTTC CTTTCAATGA AAGAGTGGGT TTCCATGATA 
CTTGATACAC TCTTGGTCAT TGTGGATAGC CATTACCAAC CCAGAGGGCA ACTTCTCTCC 
TGGTTTTATT ATAAGTCCTA TTCTGGTGAA AACACAGCTA GTGCCATTGC CCGTTCTGCT 
GCCGCCACGG CTTTGTCTCT CCTTGTGCCA GTTTTCATTA TCTCTTGCAA AGAGAAGGTT 
GAGGAAATCC TGAACATGCT GACTGCCAGG TTACCTGGGA AACCAAGTGC TGATGAGTCT 
CAAGCCGTGC AAATCCACAT GGGCCTTGCT TTAGGGATGT TTCTCTCTCG CTTGTGTGAA 
GAGAAACTCA GTGATATATG TGGCCAAGAG ATGAACCTTC TTCTGATGAA GTCGTTGGAT 
GCCCTGGAAA ATTGCTGCTT TGACACTAGT CTTGAATACA ACACGGGCTG TATATTGGGA 
GTTGGACTTG TTCTGTCCCT CATGAGTCAC AGCAGCCAAA TGCAGTCCTG CGTTCACGTG 
ACAGCATTGC TTCGGAAGCT GTCTGCATAC CTAGATGACA GTGGGAGCCA GAGCAGAACG 
TTTCAGGAGG TCCTTGCCTA CACACTTAGC TGTGTATGTA CATCAGCGTT CAGTGCTGGA 
ATTATTGAGG CTACAGAGGC TGAGGATGTT ATGAACAAGC TTCGACTGTT AGTGGAGAAT 
AGCCAGCAGA CTTCAGGTTT TGCCCTGGCT TTAGGAAACG TAGTTCATGG ATTGTCTGTG 
TGTGGACATG GAAAAGCTGA AGACTTGGGC AGCAAACTAC TCCCTGCCTG GATCAGAATT 
GTTCTAACAG AGGGCACTCC CACAATGCTT TGTCTGGCAG CTCTTCATGG CATGGTGGCC 
CTGGTAGGCT CTGAAGGGGA TGTAATGCAG CTGAAATCAG AAGCCATCCA GACCTCTCAT 
TTTCAAGGCA GACTTAATGA AGTCATTAGA ACCTTAACTC AGGTCATTAG TGTCTCTGGG 
GTGATTGGTC TCCAGTCAAA TGCAGTCTGG CTTCTTGGAC ATCTTCATCT ATCTACTCTA 
TCCTCAAGTC AAAGTAGAGC CTCTGTTCCT ACTGACTATA GCTATTTGCC TGAAAGCAGT 
TTTATTGGAG CAGCTATTGG CTTCTTCATT ACAGGAGGAA AAAAAGGTCC TGAATCTGTG 
CCTCCTTCCC TTCTTAAAGT AGTGATGAAA CCCATAGCAA CTGTTGGAGA AAGCTACCAA 
TATCCTCCTG TGAACTGGGC TGCACTTCTC TCTCCACTTA TGAGGCTAAA TTTTGGTGAA 
GAGATCCAGC AACTGTGCCT TGAAATTATG GTGACCCAGG CACAGTCATC CCAGAATGCA 
GCTGCACTAT TGGGCTTGTG GGTGACACCA CCACTGATCC ACAGTCTGAG TCTGAATACC 
AAGAGATATC TCCTGATATC TGCACCTCTG TGGATAAAAC ACATCTCTGA TGAACAGATC 
CTGGGTTTTG TTGAAAATTT AATGGTGGCA GTTTTTAAAG CAGCTTCCCC ACTTGGAAAT 
CCTGAGCTAT GCCCGAGTGC TTTACAGGGT CTGAGCCAGG CCATGAAACT GCCCAGCCCT 
GCTCACCACC TCTGGAGTCT GCTCTCTGAA GCTACTGGGA AAATTTTTGA CCTCCTGCCA 
AATAAGATTC GGAGAAAGGA TCTAGAGCTG TATATCAGCA TAGCAAAATG CCTCTTAGAA 
ATGACAGATG ATGATGCCAA TCGGATCGCC CAGGTTACTA AGAGCAACAT AGAAAAGGCT 
GCCTTTGTCA AACTGTACTT AGTCTCTCAA GGACGATTCC CCTTGGTGAA CCTGACCGAT 
ATGCTGAGTG TTGCTGTGCA GCACCGTGAG AAAGAGGTGT TGGCCTGGAT GATTCTGCAC 
AGCTTATACC AGGCACGGAT TGTGAGCCAT GCCAACACGG GCGTTTTGAA GAGAATGGAG 
TGGCTCTTGG AACTGATGGG TTATATTAGA AATGTTGCGT ACCAGTCAAC ATCCTTTCAG 
AATACGGCTC TTGATGAGGC TTTGGACTTC TTCTTGCTGA TATTTGCAAC CGCAGTGGTT 
GCATGGGCCG ACCACACTGC CCCTCTCCTC CTTGGTCTCA GTGCCAGTTG GTTGCCATGG 
CATCAGGAGA ATGGCCCGGC TGGGCCAGTA CCAAGCTTCC TTGGCAGGAG TCCAATGCAC 
AGGGTCACTG TGCAGGAGGT TCTCACTCTC CTTCCCAATA GCATGGCTCT GCTGCTGCAG 
AAAGAGCCAT GGAAGGAAGA GACCCAGAAG TTCATTGACT GGCTATTCAG CATCATGGAA 
AGCCCTAAAG AAGCCCTCTC AGCAAAGTCC AGGGATCTTT TGAAAGCCAC CCTGCTGTCC 
TTGAGAGTTC TCCCAGAGTT TAAGAAGAAA GCTGTATGGA CCAGAGCGTA TGGTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024108097.1
CDS	197..5656
Translation	MDNKGRQEAAHIHVREAKMSDDIRKRFEFPNSLIQSQAAGHLIAAVLKENGFSEKIYQSTNQTPALNLLWEKCCSDNVVVRTACCEGLVALVAQDHAEFSYVLNGILNLIPSTRNTHGLIKAIMKLLQMQALKEGQGGEKNIQSVYTIRNHPHPLITVLEHRPDCWPVFLQQLTAFFQQCPERSEVSCIQIMAPFLWYLYCEPSQLQEYAKLRLALLKVLLQPQVLCDKDQPSILQQQILQLCCDIVPCLQIKDLIQTTEAMMFIEEIYLSLLRHPVFWKIQLTQMTLQLLCVGEVSLKITGECSSSIHLLEHSVELLKEDFPVELVIIGIALLLLQTPASQQKPILNLALKLFSVTEDQKIPKSSLLLVMPILQILSSTALEDCISVDEEGPSRQQLALNLLEMIQQECYRDDHQKLSYKLVCPVTSMYGSVFTAWRILEVMTDSSAASDWLASVESLLPITTVIPVPAFLLLAHLLVEDKGQNLHQILKVTTELAQADSSQVPNLIPVLMFKLGRPLEPILYNDILYTLPKLGVHKVCIGQILRVIQLLGTTPRLRAVTLRLLTSLWEKQDRVYPELQRFMAVSDVPSLSVGKEVQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQATPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDTRPLILKTLSELFSLVPSLTVNTTEYENFKVQVLSFLWTHTQNKDPIVANAAYRSLANFSAGEHTILHLPEKIRPEIPIPEELDDDEDDEDVDLSVPGSCYLKLLSLTPPLVLPALEEFFTSLVKQEMVNMPRGIYHSALKGGVRSDQGKTVAGIPNFILKMYETNKQPGLKPGLAGGMLFCYDVSMYQSKDGKPLNRLMASRGRSFKQTSLALVHEVHIQLSEWHRAIFLPQAWLAYMNRAYHAILQGRLGELELQLKHGKEEPEEVQYKKSTAWLWVRDMLTDEITKAAAKESPVVKGNALLALSSLAVVVSRHEASVSSDSDGLLEVQPNFLSMKEWVSMILDTLLVIVDSHYQPRGQLLSWFYYKSYSGENTASAIARSAAATALSLLVPVFIISCKEKVEEILNMLTARLPGKPSADESQAVQIHMGLALGMFLSRLCEEKLSDICGQEMNLLLMKSLDALENCCFDTSLEYNTGCILGVGLVLSLMSHSSQMQSCVHVTALLRKLSAYLDDSGSQSRTFQEVLAYTLSCVCTSAFSAGIIEATEAEDVMNKLRLLVENSQQTSGFALALGNVVHGLSVCGHGKAEDLGSKLLPAWIRIVLTEGTPTMLCLAALHGMVALVGSEGDVMQLKSEAIQTSHFQGRLNEVIRTLTQVISVSGVIGLQSNAVWLLGHLHLSTLSSSQSRASVPTDYSYLPESSFIGAAIGFFITGGKKGPESVPPSLLKVVMKPIATVGESYQYPPVNWAALLSPLMRLNFGEEIQQLCLEIMVTQAQSSQNAAALLGLWVTPPLIHSLSLNTKRYLLISAPLWIKHISDEQILGFVENLMVAVFKAASPLGNPELCPSALQGLSQAMKLPSPAHHLWSLLSEATGKIFDLLPNKIRRKDLELYISIAKCLLEMTDDDANRIAQVTKSNIEKAAFVKLYLVSQGRFPLVNLTDMLSVAVQHREKEVLAWMILHSLYQARIVSHANTGVLKRMEWLLELMGYIRNVAYQSTSFQNTALDEALDFFLLIFATAVVAWADHTAPLLLGLSASWLPWHQENGPAGPVPSFLGRSPMHRVTVQEVLTLLPNSMALLLQKEPWKEETQKFIDWLFSIMESPKEALSAKSRDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_024252329.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii focadhesin (FOCAD), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024252329.1

ORF Insert Sequence:

ATGGACAACA AAGGAAGGCA AGAAGCTGCA CACATACATG TAAGAGAAGC AAAAATGTCA 
GATGATATCA GGAAAAGGTT TGAATTTCCA AATTCTCTTA TCCAATCACA GGCTGCGGGT 
CATCTTATTG CTGCAGTCCT AAAGGAGAAT GGTTTTTCAG AAAAGATTTA CCAATCTACA 
AATCAGACTC CTGCTTTGAA CTTGCTGTGG GAGAAGTGTT GCAGTGACAA TGTAGTGGTT 
CGAACAGCCT GCTGTGAAGG TCTGGTGGCA CTCGTTGCTC AGGATCATGC AGAGTTCAGC 
TATGTTCTCA ATGGGATACT CAACTTGATT CCATCAACCA GAAATACACA TGGCTTGATA 
AAAGCCATTA TGAAATTACT ACAAATGCAA GCTCTTAAGG AAGGACAAGG TGGGGAAAAG 
AATATTCAGA GTGTATATAC CATTAGAAAT CATCCTCATC CTTTGATCAC TGTGCTTGAA 
CACAGACCTG ATTGTTGGCC AGTGTTTTTG CAGCAGCTGA CAGCATTTTT CCAGCAGTGC 
CCTGAAAGGT CAGAAGTTTC ATGCATTCAA ATAATGGCAC CATTTCTGTG GTATCTGTAT 
TGTGAACCAT CTCAGTTACA AGAATATGCT AAACTCCGAC TAGCCCTGCT GAAAGTCTTA 
CTTCAACCCC AGGTTCTTTG TGACAAGGAT CAACCATCAA TACTGCAACA GCAGATACTT 
CAACTGTGTT GTGACATAGT TCCATGTTTG CAGATAAAAG ATTTGATACA GACAACAGAA 
GCGATGATGT TTATTGAGGA AATATATTTA AGCCTTTTGC GTCATCCTGT TTTCTGGAAA 
ATTCAGCTTA CCCAGATGAC TCTTCAGCTG CTGTGTGTCG GTGAAGTCAG CTTAAAGATA 
ACTGGTGAAT GTTCATCTTC AATTCACCTC TTAGAGCACA GTGTTGAACT TCTGAAGGAG 
GATTTTCCTG TTGAACTGGT CATAATTGGA ATAGCTTTAC TACTTCTACA GACTCCAGCA 
AGTCAGCAGA AGCCAATCTT AAATCTAGCT TTGAAGCTCT TCTCTGTTAC TGAGGATCAG 
AAAATCCCAA AGTCCTCTCT GCTGCTAGTG ATGCCAATTC TGCAGATACT GTCTTCTACT 
GCCTTGGAAG ACTGTATATC TGTGGATGAA GAAGGTCCCT CTAGGCAGCA GTTGGCTCTA 
AACCTTTTGG AAATGATACA GCAGGAATGT TACAGAGATG ACCACCAAAA GCTCTCCTAC 
AAGCTTGTGT GCCCTGTAAC CAGTATGTAT GGTTCAGTAT TTACAGCCTG GAGGATTCTT 
GAAGTAATGA CAGACTCCTC TGCTGCAAGT GACTGGTTGG CTTCAGTAGA GTCATTGCTT 
CCTATTACTA CTGTGATCCC TGTGCCTGCC TTTCTTCTGC TGGCTCACCT CCTTGTTGAA 
GACAAAGGAC AAAATCTTCA CCAAATACTC AAGGTCACTA CAGAATTAGC CCAAGCAGAT 
TCCTCCCAGG TGCCAAATCT GATTCCAGTT TTGATGTTCA AATTGGGAAG ACCACTGGAA 
CCTATATTAT ATAATGATAT ATTGTATACT TTACCTAAGC TTGGTGTTCA CAAGGTGTGT 
ATAGGACAAA TTCTACGAGT AATACAACTG CTTGGAACCA CACCACGACT AAGAGCTGTC 
ACTTTGCGCT TGCTGACATC TTTGTGGGAA AAGCAGGACC GAGTCTATCC TGAACTGCAG 
CGTTTCATGG CTGTGTCTGA TGTACCTTCT CTTTCGGTGG GCAAGGAAGT CCAATGGGAG 
AAACTGATTG CAAAAGCAGC ATCAATCAGA GATATATGTA AGCAGAGGCC ATATCAACAT 
GGTGCAGATA TGCTGGCAGC TATTTCTCAG GTGTTGAATG AATGCACCAA GCCTGATCAA 
GCTACTCCAG CAGCCTTGGT ATTACAAGGT CTTCATGCAC TCTGTCAAGC TGAGGTTGTT 
TGCATTCGCT CCACTTGGAA TGCTCTCTCT CCAAAGCTGA GTTGTGACAC AAGACCTCTC 
ATTCTGAAGA CACTGAGTGA ACTATTTTCT CTAGTTCCTT CCTTAACAGT CAATACAACT 
GAATATGAGA ATTTTAAAGT TCAAGTCCTC AGCTTCCTCT GGACTCATAC TCAAAACAAG 
GACCCAATTG TGGCAAATGC TGCATATAGA TCCCTGGCCA ATTTTAGTGC AGGAGAACAC 
ACCATTCTTC ATCTGCCTGA AAAGATAAGA CCAGAAATTC CCATTCCTGA AGAGTTAGAT 
GATGATGAAG ATGATGAGGA TGTGGATCTT TCAGTTCCTG GCTCTTGCTA TCTCAAACTG 
TTGTCACTCA CTCCCCCTTT GGTTTTACCA GCTTTGGAGG AATTTTTTAC ATCACTTGTG 
AAGCAAGAAA TGGTGAATAT GCCTCGTGGG ATATATCACT CTGCATTAAA AGGAGGTGTC 
CGCTCAGACC AAGGAAAGAC TGTAGCAGGA ATCCCCAATT TTATATTGAA AATGTATGAA 
ACAAACAAGC AACCAGGATT GAAACCTGGC CTTGCAGGTG GTATGTTATT TTGCTATGAT 
GTTTCCATGT ATCAGAGTAA AGATGGAAAA CCATTGAATA GACTGATGGC CAGCAGAGGG 
CGAAGTTTCA AGCAGACATC ACTTGCTCTT GTACATGAGG TTCATATCCA GCTTTCAGAG 
TGGCACCGTG CGATTTTTCT TCCACAGGCC TGGCTTGCAT ACATGAATCG AGCTTATCAT 
GCCATTTTAC AGGGAAGACT AGGAGAGCTG GAGTTGCAGT TAAAACATGG AAAAGAAGAA 
CCTGAGGAGG TGCAGTACAA AAAAAGCACA GCTTGGCTCT GGGTTAGAGA CATGCTGACT 
GATGAGATCA CCAAGGCAGC TGCAAAGGAG AGTCCGGTAG TGAAAGGCAA TGCGCTGTTA 
GCTCTAAGCA GCCTTGCTGT CGTCGTATCT AGACATGAAG CCAGCGTCTC CTCAGACTCT 
GACGGGCTCC TGGAGGTTCA ACCTAATTTC CTTTCAATGA AAGAGTGGGT TTCCATGATA 
CTTGATACAC TCTTGGTCAT TGTGGATAGC CATTACCAAC CCAGAGGGCA ACTTCTCTCC 
TGGTTTTATT ATAAGTCCTA TTCTGGTGAA AACACAGCTA GTGCCATTGC CCGTTCTGCT 
GCCGCCACGG CTTTGTCTCT CCTTGTGCCA GTTTTCATTA TCTCTTGCAA AGAGAAGGTT 
GAGGAAATCC TGAACATGCT GACTGCCAGG TTACCTGGGA AACCAAGTGC TGATGAGTCT 
CAAGCCGTGC AAATCCACAT GGGCCTTGCT TTAGGGATGT TTCTCTCTCG CTTGTGTGAA 
GAGAAACTCA GTGATATATG TGGCCAAGAG ATGAACCTTC TTCTGATGAA GTCGTTGGAT 
GCCCTGGAAA ATTGCTGCTT TGACACTAGT CTTGAATACA ACACGGGCTG TATATTGGGA 
GTTGGACTTG TTCTGTCCCT CATGAGTCAC AGCAGCCAAA TGCAGTCCTG CGTTCACGTG 
ACAGCATTGC TTCGGAAGCT GTCTGCATAC CTAGATGACA GTGGGAGCCA GAGCAGAACG 
TTTCAGGAGG TCCTTGCCTA CACACTTAGC TGTGTATGTA CATCAGCGTT CAGTGCTGGA 
ATTATTGAGG CTACAGAGGC TGAGGATGTT ATGAACAAGC TTCGACTGTT AGTGGAGAAT 
AGCCAGCAGA CTTCAGGTTT TGCCCTGGCT TTAGGAAACG TAGTTCATGG ATTGTCTGTG 
TGTGGACATG GAAAAGCTGA AGACTTGGGC AGCAAACTAC TCCCTGCCTG GATCAGAATT 
GTTCTAACAG AGGGCACTCC CACAATGCTT TGTCTGGCAG CTCTTCATGG CATGGTGGCC 
CTGGTAGGCT CTGAAGGGGA TGTAATGCAG CTGAAATCAG AAGCCATCCA GACCTCTCAT 
TTTCAAGGCA GACTTAATGA AGTCATTAGA ACCTTAACTC AGGTCATTAG TGTCTCTGGG 
GTGATTGGTC TCCAGTCAAA TGCAGTCTGG CTTCTTGGAC ATCTTCATCT ATCTACTCTA 
TCCTCAAGTC AAAGTAGAGC CTCTGTTCCT ACTGACTATA GCTATTTGCC TGAAAGCAGT 
TTTATTGGAG CAGCTATTGG CTTCTTCATT ACAGGAGGAA AAAAAGGTCC TGAATCTGTG 
CCTCCTTCCC TTCTTAAAGT AGTGATGAAA CCCATAGCAA CTGTTGGAGA AAGCTACCAA 
TATCCTCCTG TGAACTGGGC TGCACTTCTC TCTCCACTTA TGAGGCTAAA TTTTGGTGAA 
GAGATCCAGC AACTGTGCCT TGAAATTATG GTGACCCAGG CACAGTCATC CCAGAATGCA 
GCTGCACTAT TGGGCTTGTG GGTGACACCA CCACTGATCC ACAGTCTGAG TCTGAATACC 
AAGAGATATC TCCTGATATC TGCACCTCTG TGGATAAAAC ACATCTCTGA TGAACAGATC 
CTGGGTTTTG TTGAAAATTT AATGGTGGCA GTTTTTAAAG CAGCTTCCCC ACTTGGAAAT 
CCTGAGCTAT GCCCGAGTGC TTTACAGGGT CTGAGCCAGG CCATGAAACT GCCCAGCCCT 
GCTCACCACC TCTGGAGTCT GCTCTCTGAA GCTACTGGGA AAATTTTTGA CCTCCTGCCA 
AATAAGATTC GGAGAAAGGA TCTAGAGCTG TATATCAGCA TAGCAAAATG CCTCTTAGAA 
ATGACAGATG ATGATGCCAA TCGGATCGCC CAGGTTACTA AGAGCAACAT AGAAAAGGCT 
GCCTTTGTCA AACTGTACTT AGTCTCTCAA GGACGATTCC CCTTGGTGAA CCTGACCGAT 
ATGCTGAGTG TTGCTGTGCA GCACCGTGAG AAAGAGGTGT TGGCCTGGAT GATTCTGCAC 
AGCTTATACC AGGCACGGAT TGTGAGCCAT GCCAACACGG GCGTTTTGAA GAGAATGGAG 
TGGCTCTTGG AACTGATGGG TTATATTAGA AATGTTGCGT ACCAGTCAAC ATCCTTTCAG 
AATACGGCTC TTGATGAGGC TTTGGACTTC TTCTTGCTGA TATTTGCAAC CGCAGTGGTT 
GCATGGGCCG ACCACACTGC CCCTCTCCTC CTTGGTCTCA GTGCCAGTTG GTTGCCATGG 
CATCAGGAGA ATGGCCCGGC TGGGCCAGTA CCAAGCTTCC TTGGCAGGAG TCCAATGCAC 
AGGGTCACTG TGCAGGAGGT TCTCACTCTC CTTCCCAATA GCATGGCTCT GCTGCTGCAG 
AAAGAGCCAT GGAAGGAAGA GACCCAGAAG TTCATTGACT GGCTATTCAG CATCATGGAA 
AGCCCTAAAG AAGCCCTCTC AGCAAAGTCC AGGGATCTTT TGAAAGCCAC CCTGCTGTCC 
TTGAGAGTTC TCCCAGAGTT TAAGAAGAAA GCTGTATGGA CCAGAGCGTA TGGTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FOCAD ( XM_024252330.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024108098.1 Pongo abelii focadhesin (FOCAD), transcript variant X4, mRNA.

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CloneID	OSa186755
Clone ID Related Accession (Same CDS sequence)	XM_024252329.1 , XM_024252330.1
Accession Version	XM_024252330.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	focadhesin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036912.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAACA AAGGAAGGCA AGAAGCTGCA CACATACATG TAAGAGAAGC AAAAATGTCA 
GATGATATCA GGAAAAGGTT TGAATTTCCA AATTCTCTTA TCCAATCACA GGCTGCGGGT 
CATCTTATTG CTGCAGTCCT AAAGGAGAAT GGTTTTTCAG AAAAGATTTA CCAATCTACA 
AATCAGACTC CTGCTTTGAA CTTGCTGTGG GAGAAGTGTT GCAGTGACAA TGTAGTGGTT 
CGAACAGCCT GCTGTGAAGG TCTGGTGGCA CTCGTTGCTC AGGATCATGC AGAGTTCAGC 
TATGTTCTCA ATGGGATACT CAACTTGATT CCATCAACCA GAAATACACA TGGCTTGATA 
AAAGCCATTA TGAAATTACT ACAAATGCAA GCTCTTAAGG AAGGACAAGG TGGGGAAAAG 
AATATTCAGA GTGTATATAC CATTAGAAAT CATCCTCATC CTTTGATCAC TGTGCTTGAA 
CACAGACCTG ATTGTTGGCC AGTGTTTTTG CAGCAGCTGA CAGCATTTTT CCAGCAGTGC 
CCTGAAAGGT CAGAAGTTTC ATGCATTCAA ATAATGGCAC CATTTCTGTG GTATCTGTAT 
TGTGAACCAT CTCAGTTACA AGAATATGCT AAACTCCGAC TAGCCCTGCT GAAAGTCTTA 
CTTCAACCCC AGGTTCTTTG TGACAAGGAT CAACCATCAA TACTGCAACA GCAGATACTT 
CAACTGTGTT GTGACATAGT TCCATGTTTG CAGATAAAAG ATTTGATACA GACAACAGAA 
GCGATGATGT TTATTGAGGA AATATATTTA AGCCTTTTGC GTCATCCTGT TTTCTGGAAA 
ATTCAGCTTA CCCAGATGAC TCTTCAGCTG CTGTGTGTCG GTGAAGTCAG CTTAAAGATA 
ACTGGTGAAT GTTCATCTTC AATTCACCTC TTAGAGCACA GTGTTGAACT TCTGAAGGAG 
GATTTTCCTG TTGAACTGGT CATAATTGGA ATAGCTTTAC TACTTCTACA GACTCCAGCA 
AGTCAGCAGA AGCCAATCTT AAATCTAGCT TTGAAGCTCT TCTCTGTTAC TGAGGATCAG 
AAAATCCCAA AGTCCTCTCT GCTGCTAGTG ATGCCAATTC TGCAGATACT GTCTTCTACT 
GCCTTGGAAG ACTGTATATC TGTGGATGAA GAAGGTCCCT CTAGGCAGCA GTTGGCTCTA 
AACCTTTTGG AAATGATACA GCAGGAATGT TACAGAGATG ACCACCAAAA GCTCTCCTAC 
AAGCTTGTGT GCCCTGTAAC CAGTATGTAT GGTTCAGTAT TTACAGCCTG GAGGATTCTT 
GAAGTAATGA CAGACTCCTC TGCTGCAAGT GACTGGTTGG CTTCAGTAGA GTCATTGCTT 
CCTATTACTA CTGTGATCCC TGTGCCTGCC TTTCTTCTGC TGGCTCACCT CCTTGTTGAA 
GACAAAGGAC AAAATCTTCA CCAAATACTC AAGGTCACTA CAGAATTAGC CCAAGCAGAT 
TCCTCCCAGG TGCCAAATCT GATTCCAGTT TTGATGTTCA AATTGGGAAG ACCACTGGAA 
CCTATATTAT ATAATGATAT ATTGTATACT TTACCTAAGC TTGGTGTTCA CAAGGTGTGT 
ATAGGACAAA TTCTACGAGT AATACAACTG CTTGGAACCA CACCACGACT AAGAGCTGTC 
ACTTTGCGCT TGCTGACATC TTTGTGGGAA AAGCAGGACC GAGTCTATCC TGAACTGCAG 
CGTTTCATGG CTGTGTCTGA TGTACCTTCT CTTTCGGTGG GCAAGGAAGT CCAATGGGAG 
AAACTGATTG CAAAAGCAGC ATCAATCAGA GATATATGTA AGCAGAGGCC ATATCAACAT 
GGTGCAGATA TGCTGGCAGC TATTTCTCAG GTGTTGAATG AATGCACCAA GCCTGATCAA 
GCTACTCCAG CAGCCTTGGT ATTACAAGGT CTTCATGCAC TCTGTCAAGC TGAGGTTGTT 
TGCATTCGCT CCACTTGGAA TGCTCTCTCT CCAAAGCTGA GTTGTGACAC AAGACCTCTC 
ATTCTGAAGA CACTGAGTGA ACTATTTTCT CTAGTTCCTT CCTTAACAGT CAATACAACT 
GAATATGAGA ATTTTAAAGT TCAAGTCCTC AGCTTCCTCT GGACTCATAC TCAAAACAAG 
GACCCAATTG TGGCAAATGC TGCATATAGA TCCCTGGCCA ATTTTAGTGC AGGAGAACAC 
ACCATTCTTC ATCTGCCTGA AAAGATAAGA CCAGAAATTC CCATTCCTGA AGAGTTAGAT 
GATGATGAAG ATGATGAGGA TGTGGATCTT TCAGTTCCTG GCTCTTGCTA TCTCAAACTG 
TTGTCACTCA CTCCCCCTTT GGTTTTACCA GCTTTGGAGG AATTTTTTAC ATCACTTGTG 
AAGCAAGAAA TGGTGAATAT GCCTCGTGGG ATATATCACT CTGCATTAAA AGGAGGTGTC 
CGCTCAGACC AAGGAAAGAC TGTAGCAGGA ATCCCCAATT TTATATTGAA AATGTATGAA 
ACAAACAAGC AACCAGGATT GAAACCTGGC CTTGCAGGTG GTATGTTATT TTGCTATGAT 
GTTTCCATGT ATCAGAGTAA AGATGGAAAA CCATTGAATA GACTGATGGC CAGCAGAGGG 
CGAAGTTTCA AGCAGACATC ACTTGCTCTT GTACATGAGG TTCATATCCA GCTTTCAGAG 
TGGCACCGTG CGATTTTTCT TCCACAGGCC TGGCTTGCAT ACATGAATCG AGCTTATCAT 
GCCATTTTAC AGGGAAGACT AGGAGAGCTG GAGTTGCAGT TAAAACATGG AAAAGAAGAA 
CCTGAGGAGG TGCAGTACAA AAAAAGCACA GCTTGGCTCT GGGTTAGAGA CATGCTGACT 
GATGAGATCA CCAAGGCAGC TGCAAAGGAG AGTCCGGTAG TGAAAGGCAA TGCGCTGTTA 
GCTCTAAGCA GCCTTGCTGT CGTCGTATCT AGACATGAAG CCAGCGTCTC CTCAGACTCT 
GACGGGCTCC TGGAGGTTCA ACCTAATTTC CTTTCAATGA AAGAGTGGGT TTCCATGATA 
CTTGATACAC TCTTGGTCAT TGTGGATAGC CATTACCAAC CCAGAGGGCA ACTTCTCTCC 
TGGTTTTATT ATAAGTCCTA TTCTGGTGAA AACACAGCTA GTGCCATTGC CCGTTCTGCT 
GCCGCCACGG CTTTGTCTCT CCTTGTGCCA GTTTTCATTA TCTCTTGCAA AGAGAAGGTT 
GAGGAAATCC TGAACATGCT GACTGCCAGG TTACCTGGGA AACCAAGTGC TGATGAGTCT 
CAAGCCGTGC AAATCCACAT GGGCCTTGCT TTAGGGATGT TTCTCTCTCG CTTGTGTGAA 
GAGAAACTCA GTGATATATG TGGCCAAGAG ATGAACCTTC TTCTGATGAA GTCGTTGGAT 
GCCCTGGAAA ATTGCTGCTT TGACACTAGT CTTGAATACA ACACGGGCTG TATATTGGGA 
GTTGGACTTG TTCTGTCCCT CATGAGTCAC AGCAGCCAAA TGCAGTCCTG CGTTCACGTG 
ACAGCATTGC TTCGGAAGCT GTCTGCATAC CTAGATGACA GTGGGAGCCA GAGCAGAACG 
TTTCAGGAGG TCCTTGCCTA CACACTTAGC TGTGTATGTA CATCAGCGTT CAGTGCTGGA 
ATTATTGAGG CTACAGAGGC TGAGGATGTT ATGAACAAGC TTCGACTGTT AGTGGAGAAT 
AGCCAGCAGA CTTCAGGTTT TGCCCTGGCT TTAGGAAACG TAGTTCATGG ATTGTCTGTG 
TGTGGACATG GAAAAGCTGA AGACTTGGGC AGCAAACTAC TCCCTGCCTG GATCAGAATT 
GTTCTAACAG AGGGCACTCC CACAATGCTT TGTCTGGCAG CTCTTCATGG CATGGTGGCC 
CTGGTAGGCT CTGAAGGGGA TGTAATGCAG CTGAAATCAG AAGCCATCCA GACCTCTCAT 
TTTCAAGGCA GACTTAATGA AGTCATTAGA ACCTTAACTC AGGTCATTAG TGTCTCTGGG 
GTGATTGGTC TCCAGTCAAA TGCAGTCTGG CTTCTTGGAC ATCTTCATCT ATCTACTCTA 
TCCTCAAGTC AAAGTAGAGC CTCTGTTCCT ACTGACTATA GCTATTTGCC TGAAAGCAGT 
TTTATTGGAG CAGCTATTGG CTTCTTCATT ACAGGAGGAA AAAAAGGTCC TGAATCTGTG 
CCTCCTTCCC TTCTTAAAGT AGTGATGAAA CCCATAGCAA CTGTTGGAGA AAGCTACCAA 
TATCCTCCTG TGAACTGGGC TGCACTTCTC TCTCCACTTA TGAGGCTAAA TTTTGGTGAA 
GAGATCCAGC AACTGTGCCT TGAAATTATG GTGACCCAGG CACAGTCATC CCAGAATGCA 
GCTGCACTAT TGGGCTTGTG GGTGACACCA CCACTGATCC ACAGTCTGAG TCTGAATACC 
AAGAGATATC TCCTGATATC TGCACCTCTG TGGATAAAAC ACATCTCTGA TGAACAGATC 
CTGGGTTTTG TTGAAAATTT AATGGTGGCA GTTTTTAAAG CAGCTTCCCC ACTTGGAAAT 
CCTGAGCTAT GCCCGAGTGC TTTACAGGGT CTGAGCCAGG CCATGAAACT GCCCAGCCCT 
GCTCACCACC TCTGGAGTCT GCTCTCTGAA GCTACTGGGA AAATTTTTGA CCTCCTGCCA 
AATAAGATTC GGAGAAAGGA TCTAGAGCTG TATATCAGCA TAGCAAAATG CCTCTTAGAA 
ATGACAGATG ATGATGCCAA TCGGATCGCC CAGGTTACTA AGAGCAACAT AGAAAAGGCT 
GCCTTTGTCA AACTGTACTT AGTCTCTCAA GGACGATTCC CCTTGGTGAA CCTGACCGAT 
ATGCTGAGTG TTGCTGTGCA GCACCGTGAG AAAGAGGTGT TGGCCTGGAT GATTCTGCAC 
AGCTTATACC AGGCACGGAT TGTGAGCCAT GCCAACACGG GCGTTTTGAA GAGAATGGAG 
TGGCTCTTGG AACTGATGGG TTATATTAGA AATGTTGCGT ACCAGTCAAC ATCCTTTCAG 
AATACGGCTC TTGATGAGGC TTTGGACTTC TTCTTGCTGA TATTTGCAAC CGCAGTGGTT 
GCATGGGCCG ACCACACTGC CCCTCTCCTC CTTGGTCTCA GTGCCAGTTG GTTGCCATGG 
CATCAGGAGA ATGGCCCGGC TGGGCCAGTA CCAAGCTTCC TTGGCAGGAG TCCAATGCAC 
AGGGTCACTG TGCAGGAGGT TCTCACTCTC CTTCCCAATA GCATGGCTCT GCTGCTGCAG 
AAAGAGCCAT GGAAGGAAGA GACCCAGAAG TTCATTGACT GGCTATTCAG CATCATGGAA 
AGCCCTAAAG AAGCCCTCTC AGCAAAGTCC AGGGATCTTT TGAAAGCCAC CCTGCTGTCC 
TTGAGAGTTC TCCCAGAGTT TAAGAAGAAA GCTGTATGGA CCAGAGCGTA TGGTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024108098.1
CDS	136..5595
Translation	MDNKGRQEAAHIHVREAKMSDDIRKRFEFPNSLIQSQAAGHLIAAVLKENGFSEKIYQSTNQTPALNLLWEKCCSDNVVVRTACCEGLVALVAQDHAEFSYVLNGILNLIPSTRNTHGLIKAIMKLLQMQALKEGQGGEKNIQSVYTIRNHPHPLITVLEHRPDCWPVFLQQLTAFFQQCPERSEVSCIQIMAPFLWYLYCEPSQLQEYAKLRLALLKVLLQPQVLCDKDQPSILQQQILQLCCDIVPCLQIKDLIQTTEAMMFIEEIYLSLLRHPVFWKIQLTQMTLQLLCVGEVSLKITGECSSSIHLLEHSVELLKEDFPVELVIIGIALLLLQTPASQQKPILNLALKLFSVTEDQKIPKSSLLLVMPILQILSSTALEDCISVDEEGPSRQQLALNLLEMIQQECYRDDHQKLSYKLVCPVTSMYGSVFTAWRILEVMTDSSAASDWLASVESLLPITTVIPVPAFLLLAHLLVEDKGQNLHQILKVTTELAQADSSQVPNLIPVLMFKLGRPLEPILYNDILYTLPKLGVHKVCIGQILRVIQLLGTTPRLRAVTLRLLTSLWEKQDRVYPELQRFMAVSDVPSLSVGKEVQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQATPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDTRPLILKTLSELFSLVPSLTVNTTEYENFKVQVLSFLWTHTQNKDPIVANAAYRSLANFSAGEHTILHLPEKIRPEIPIPEELDDDEDDEDVDLSVPGSCYLKLLSLTPPLVLPALEEFFTSLVKQEMVNMPRGIYHSALKGGVRSDQGKTVAGIPNFILKMYETNKQPGLKPGLAGGMLFCYDVSMYQSKDGKPLNRLMASRGRSFKQTSLALVHEVHIQLSEWHRAIFLPQAWLAYMNRAYHAILQGRLGELELQLKHGKEEPEEVQYKKSTAWLWVRDMLTDEITKAAAKESPVVKGNALLALSSLAVVVSRHEASVSSDSDGLLEVQPNFLSMKEWVSMILDTLLVIVDSHYQPRGQLLSWFYYKSYSGENTASAIARSAAATALSLLVPVFIISCKEKVEEILNMLTARLPGKPSADESQAVQIHMGLALGMFLSRLCEEKLSDICGQEMNLLLMKSLDALENCCFDTSLEYNTGCILGVGLVLSLMSHSSQMQSCVHVTALLRKLSAYLDDSGSQSRTFQEVLAYTLSCVCTSAFSAGIIEATEAEDVMNKLRLLVENSQQTSGFALALGNVVHGLSVCGHGKAEDLGSKLLPAWIRIVLTEGTPTMLCLAALHGMVALVGSEGDVMQLKSEAIQTSHFQGRLNEVIRTLTQVISVSGVIGLQSNAVWLLGHLHLSTLSSSQSRASVPTDYSYLPESSFIGAAIGFFITGGKKGPESVPPSLLKVVMKPIATVGESYQYPPVNWAALLSPLMRLNFGEEIQQLCLEIMVTQAQSSQNAAALLGLWVTPPLIHSLSLNTKRYLLISAPLWIKHISDEQILGFVENLMVAVFKAASPLGNPELCPSALQGLSQAMKLPSPAHHLWSLLSEATGKIFDLLPNKIRRKDLELYISIAKCLLEMTDDDANRIAQVTKSNIEKAAFVKLYLVSQGRFPLVNLTDMLSVAVQHREKEVLAWMILHSLYQARIVSHANTGVLKRMEWLLELMGYIRNVAYQSTSFQNTALDEALDFFLLIFATAVVAWADHTAPLLLGLSASWLPWHQENGPAGPVPSFLGRSPMHRVTVQEVLTLLPNSMALLLQKEPWKEETQKFIDWLFSIMESPKEALSAKSRDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_024252330.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii focadhesin (FOCAD), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024252330.1

ORF Insert Sequence:

ATGGACAACA AAGGAAGGCA AGAAGCTGCA CACATACATG TAAGAGAAGC AAAAATGTCA 
GATGATATCA GGAAAAGGTT TGAATTTCCA AATTCTCTTA TCCAATCACA GGCTGCGGGT 
CATCTTATTG CTGCAGTCCT AAAGGAGAAT GGTTTTTCAG AAAAGATTTA CCAATCTACA 
AATCAGACTC CTGCTTTGAA CTTGCTGTGG GAGAAGTGTT GCAGTGACAA TGTAGTGGTT 
CGAACAGCCT GCTGTGAAGG TCTGGTGGCA CTCGTTGCTC AGGATCATGC AGAGTTCAGC 
TATGTTCTCA ATGGGATACT CAACTTGATT CCATCAACCA GAAATACACA TGGCTTGATA 
AAAGCCATTA TGAAATTACT ACAAATGCAA GCTCTTAAGG AAGGACAAGG TGGGGAAAAG 
AATATTCAGA GTGTATATAC CATTAGAAAT CATCCTCATC CTTTGATCAC TGTGCTTGAA 
CACAGACCTG ATTGTTGGCC AGTGTTTTTG CAGCAGCTGA CAGCATTTTT CCAGCAGTGC 
CCTGAAAGGT CAGAAGTTTC ATGCATTCAA ATAATGGCAC CATTTCTGTG GTATCTGTAT 
TGTGAACCAT CTCAGTTACA AGAATATGCT AAACTCCGAC TAGCCCTGCT GAAAGTCTTA 
CTTCAACCCC AGGTTCTTTG TGACAAGGAT CAACCATCAA TACTGCAACA GCAGATACTT 
CAACTGTGTT GTGACATAGT TCCATGTTTG CAGATAAAAG ATTTGATACA GACAACAGAA 
GCGATGATGT TTATTGAGGA AATATATTTA AGCCTTTTGC GTCATCCTGT TTTCTGGAAA 
ATTCAGCTTA CCCAGATGAC TCTTCAGCTG CTGTGTGTCG GTGAAGTCAG CTTAAAGATA 
ACTGGTGAAT GTTCATCTTC AATTCACCTC TTAGAGCACA GTGTTGAACT TCTGAAGGAG 
GATTTTCCTG TTGAACTGGT CATAATTGGA ATAGCTTTAC TACTTCTACA GACTCCAGCA 
AGTCAGCAGA AGCCAATCTT AAATCTAGCT TTGAAGCTCT TCTCTGTTAC TGAGGATCAG 
AAAATCCCAA AGTCCTCTCT GCTGCTAGTG ATGCCAATTC TGCAGATACT GTCTTCTACT 
GCCTTGGAAG ACTGTATATC TGTGGATGAA GAAGGTCCCT CTAGGCAGCA GTTGGCTCTA 
AACCTTTTGG AAATGATACA GCAGGAATGT TACAGAGATG ACCACCAAAA GCTCTCCTAC 
AAGCTTGTGT GCCCTGTAAC CAGTATGTAT GGTTCAGTAT TTACAGCCTG GAGGATTCTT 
GAAGTAATGA CAGACTCCTC TGCTGCAAGT GACTGGTTGG CTTCAGTAGA GTCATTGCTT 
CCTATTACTA CTGTGATCCC TGTGCCTGCC TTTCTTCTGC TGGCTCACCT CCTTGTTGAA 
GACAAAGGAC AAAATCTTCA CCAAATACTC AAGGTCACTA CAGAATTAGC CCAAGCAGAT 
TCCTCCCAGG TGCCAAATCT GATTCCAGTT TTGATGTTCA AATTGGGAAG ACCACTGGAA 
CCTATATTAT ATAATGATAT ATTGTATACT TTACCTAAGC TTGGTGTTCA CAAGGTGTGT 
ATAGGACAAA TTCTACGAGT AATACAACTG CTTGGAACCA CACCACGACT AAGAGCTGTC 
ACTTTGCGCT TGCTGACATC TTTGTGGGAA AAGCAGGACC GAGTCTATCC TGAACTGCAG 
CGTTTCATGG CTGTGTCTGA TGTACCTTCT CTTTCGGTGG GCAAGGAAGT CCAATGGGAG 
AAACTGATTG CAAAAGCAGC ATCAATCAGA GATATATGTA AGCAGAGGCC ATATCAACAT 
GGTGCAGATA TGCTGGCAGC TATTTCTCAG GTGTTGAATG AATGCACCAA GCCTGATCAA 
GCTACTCCAG CAGCCTTGGT ATTACAAGGT CTTCATGCAC TCTGTCAAGC TGAGGTTGTT 
TGCATTCGCT CCACTTGGAA TGCTCTCTCT CCAAAGCTGA GTTGTGACAC AAGACCTCTC 
ATTCTGAAGA CACTGAGTGA ACTATTTTCT CTAGTTCCTT CCTTAACAGT CAATACAACT 
GAATATGAGA ATTTTAAAGT TCAAGTCCTC AGCTTCCTCT GGACTCATAC TCAAAACAAG 
GACCCAATTG TGGCAAATGC TGCATATAGA TCCCTGGCCA ATTTTAGTGC AGGAGAACAC 
ACCATTCTTC ATCTGCCTGA AAAGATAAGA CCAGAAATTC CCATTCCTGA AGAGTTAGAT 
GATGATGAAG ATGATGAGGA TGTGGATCTT TCAGTTCCTG GCTCTTGCTA TCTCAAACTG 
TTGTCACTCA CTCCCCCTTT GGTTTTACCA GCTTTGGAGG AATTTTTTAC ATCACTTGTG 
AAGCAAGAAA TGGTGAATAT GCCTCGTGGG ATATATCACT CTGCATTAAA AGGAGGTGTC 
CGCTCAGACC AAGGAAAGAC TGTAGCAGGA ATCCCCAATT TTATATTGAA AATGTATGAA 
ACAAACAAGC AACCAGGATT GAAACCTGGC CTTGCAGGTG GTATGTTATT TTGCTATGAT 
GTTTCCATGT ATCAGAGTAA AGATGGAAAA CCATTGAATA GACTGATGGC CAGCAGAGGG 
CGAAGTTTCA AGCAGACATC ACTTGCTCTT GTACATGAGG TTCATATCCA GCTTTCAGAG 
TGGCACCGTG CGATTTTTCT TCCACAGGCC TGGCTTGCAT ACATGAATCG AGCTTATCAT 
GCCATTTTAC AGGGAAGACT AGGAGAGCTG GAGTTGCAGT TAAAACATGG AAAAGAAGAA 
CCTGAGGAGG TGCAGTACAA AAAAAGCACA GCTTGGCTCT GGGTTAGAGA CATGCTGACT 
GATGAGATCA CCAAGGCAGC TGCAAAGGAG AGTCCGGTAG TGAAAGGCAA TGCGCTGTTA 
GCTCTAAGCA GCCTTGCTGT CGTCGTATCT AGACATGAAG CCAGCGTCTC CTCAGACTCT 
GACGGGCTCC TGGAGGTTCA ACCTAATTTC CTTTCAATGA AAGAGTGGGT TTCCATGATA 
CTTGATACAC TCTTGGTCAT TGTGGATAGC CATTACCAAC CCAGAGGGCA ACTTCTCTCC 
TGGTTTTATT ATAAGTCCTA TTCTGGTGAA AACACAGCTA GTGCCATTGC CCGTTCTGCT 
GCCGCCACGG CTTTGTCTCT CCTTGTGCCA GTTTTCATTA TCTCTTGCAA AGAGAAGGTT 
GAGGAAATCC TGAACATGCT GACTGCCAGG TTACCTGGGA AACCAAGTGC TGATGAGTCT 
CAAGCCGTGC AAATCCACAT GGGCCTTGCT TTAGGGATGT TTCTCTCTCG CTTGTGTGAA 
GAGAAACTCA GTGATATATG TGGCCAAGAG ATGAACCTTC TTCTGATGAA GTCGTTGGAT 
GCCCTGGAAA ATTGCTGCTT TGACACTAGT CTTGAATACA ACACGGGCTG TATATTGGGA 
GTTGGACTTG TTCTGTCCCT CATGAGTCAC AGCAGCCAAA TGCAGTCCTG CGTTCACGTG 
ACAGCATTGC TTCGGAAGCT GTCTGCATAC CTAGATGACA GTGGGAGCCA GAGCAGAACG 
TTTCAGGAGG TCCTTGCCTA CACACTTAGC TGTGTATGTA CATCAGCGTT CAGTGCTGGA 
ATTATTGAGG CTACAGAGGC TGAGGATGTT ATGAACAAGC TTCGACTGTT AGTGGAGAAT 
AGCCAGCAGA CTTCAGGTTT TGCCCTGGCT TTAGGAAACG TAGTTCATGG ATTGTCTGTG 
TGTGGACATG GAAAAGCTGA AGACTTGGGC AGCAAACTAC TCCCTGCCTG GATCAGAATT 
GTTCTAACAG AGGGCACTCC CACAATGCTT TGTCTGGCAG CTCTTCATGG CATGGTGGCC 
CTGGTAGGCT CTGAAGGGGA TGTAATGCAG CTGAAATCAG AAGCCATCCA GACCTCTCAT 
TTTCAAGGCA GACTTAATGA AGTCATTAGA ACCTTAACTC AGGTCATTAG TGTCTCTGGG 
GTGATTGGTC TCCAGTCAAA TGCAGTCTGG CTTCTTGGAC ATCTTCATCT ATCTACTCTA 
TCCTCAAGTC AAAGTAGAGC CTCTGTTCCT ACTGACTATA GCTATTTGCC TGAAAGCAGT 
TTTATTGGAG CAGCTATTGG CTTCTTCATT ACAGGAGGAA AAAAAGGTCC TGAATCTGTG 
CCTCCTTCCC TTCTTAAAGT AGTGATGAAA CCCATAGCAA CTGTTGGAGA AAGCTACCAA 
TATCCTCCTG TGAACTGGGC TGCACTTCTC TCTCCACTTA TGAGGCTAAA TTTTGGTGAA 
GAGATCCAGC AACTGTGCCT TGAAATTATG GTGACCCAGG CACAGTCATC CCAGAATGCA 
GCTGCACTAT TGGGCTTGTG GGTGACACCA CCACTGATCC ACAGTCTGAG TCTGAATACC 
AAGAGATATC TCCTGATATC TGCACCTCTG TGGATAAAAC ACATCTCTGA TGAACAGATC 
CTGGGTTTTG TTGAAAATTT AATGGTGGCA GTTTTTAAAG CAGCTTCCCC ACTTGGAAAT 
CCTGAGCTAT GCCCGAGTGC TTTACAGGGT CTGAGCCAGG CCATGAAACT GCCCAGCCCT 
GCTCACCACC TCTGGAGTCT GCTCTCTGAA GCTACTGGGA AAATTTTTGA CCTCCTGCCA 
AATAAGATTC GGAGAAAGGA TCTAGAGCTG TATATCAGCA TAGCAAAATG CCTCTTAGAA 
ATGACAGATG ATGATGCCAA TCGGATCGCC CAGGTTACTA AGAGCAACAT AGAAAAGGCT 
GCCTTTGTCA AACTGTACTT AGTCTCTCAA GGACGATTCC CCTTGGTGAA CCTGACCGAT 
ATGCTGAGTG TTGCTGTGCA GCACCGTGAG AAAGAGGTGT TGGCCTGGAT GATTCTGCAC 
AGCTTATACC AGGCACGGAT TGTGAGCCAT GCCAACACGG GCGTTTTGAA GAGAATGGAG 
TGGCTCTTGG AACTGATGGG TTATATTAGA AATGTTGCGT ACCAGTCAAC ATCCTTTCAG 
AATACGGCTC TTGATGAGGC TTTGGACTTC TTCTTGCTGA TATTTGCAAC CGCAGTGGTT 
GCATGGGCCG ACCACACTGC CCCTCTCCTC CTTGGTCTCA GTGCCAGTTG GTTGCCATGG 
CATCAGGAGA ATGGCCCGGC TGGGCCAGTA CCAAGCTTCC TTGGCAGGAG TCCAATGCAC 
AGGGTCACTG TGCAGGAGGT TCTCACTCTC CTTCCCAATA GCATGGCTCT GCTGCTGCAG 
AAAGAGCCAT GGAAGGAAGA GACCCAGAAG TTCATTGACT GGCTATTCAG CATCATGGAA 
AGCCCTAAAG AAGCCCTCTC AGCAAAGTCC AGGGATCTTT TGAAAGCCAC CCTGCTGTCC 
TTGAGAGTTC TCCCAGAGTT TAAGAAGAAA GCTGTATGGA CCAGAGCGTA TGGTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FOCAD ( XM_009244392.2 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009242667.2 Pongo abelii focadhesin (FOCAD), transcript variant X5, mRNA.

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CloneID	OSa186756
Clone ID Related Accession (Same CDS sequence)	XM_009244392.2 , XM_024252331.1 , XM_024252332.1 , XM_024252334.1 , XM_009244394.2
Accession Version	XM_009244392.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	focadhesin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036912.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 28, 2018 this sequence version replaced XM_009244392.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGATG ATATCAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA ATCACAGGCT 
GCGGGTCATC TTATTGCTGC AGTCCTAAAG GAGAATGGTT TTTCAGAAAA GATTTACCAA 
TCTACAAATC AGACTCCTGC TTTGAACTTG CTGTGGGAGA AGTGTTGCAG TGACAATGTA 
GTGGTTCGAA CAGCCTGCTG TGAAGGTCTG GTGGCACTCG TTGCTCAGGA TCATGCAGAG 
TTCAGCTATG TTCTCAATGG GATACTCAAC TTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCATTATGAA ATTACTACAA ATGCAAGCTC TTAAGGAAGG ACAAGGTGGG 
GAAAAGAATA TTCAGAGTGT ATATACCATT AGAAATCATC CTCATCCTTT GATCACTGTG 
CTTGAACACA GACCTGATTG TTGGCCAGTG TTTTTGCAGC AGCTGACAGC ATTTTTCCAG 
CAGTGCCCTG AAAGGTCAGA AGTTTCATGC ATTCAAATAA TGGCACCATT TCTGTGGTAT 
CTGTATTGTG AACCATCTCA GTTACAAGAA TATGCTAAAC TCCGACTAGC CCTGCTGAAA 
GTCTTACTTC AACCCCAGGT TCTTTGTGAC AAGGATCAAC CATCAATACT GCAACAGCAG 
ATACTTCAAC TGTGTTGTGA CATAGTTCCA TGTTTGCAGA TAAAAGATTT GATACAGACA 
ACAGAAGCGA TGATGTTTAT TGAGGAAATA TATTTAAGCC TTTTGCGTCA TCCTGTTTTC 
TGGAAAATTC AGCTTACCCA GATGACTCTT CAGCTGCTGT GTGTCGGTGA AGTCAGCTTA 
AAGATAACTG GTGAATGTTC ATCTTCAATT CACCTCTTAG AGCACAGTGT TGAACTTCTG 
AAGGAGGATT TTCCTGTTGA ACTGGTCATA ATTGGAATAG CTTTACTACT TCTACAGACT 
CCAGCAAGTC AGCAGAAGCC AATCTTAAAT CTAGCTTTGA AGCTCTTCTC TGTTACTGAG 
GATCAGAAAA TCCCAAAGTC CTCTCTGCTG CTAGTGATGC CAATTCTGCA GATACTGTCT 
TCTACTGCCT TGGAAGACTG TATATCTGTG GATGAAGAAG GTCCCTCTAG GCAGCAGTTG 
GCTCTAAACC TTTTGGAAAT GATACAGCAG GAATGTTACA GAGATGACCA CCAAAAGCTC 
TCCTACAAGC TTGTGTGCCC TGTAACCAGT ATGTATGGTT CAGTATTTAC AGCCTGGAGG 
ATTCTTGAAG TAATGACAGA CTCCTCTGCT GCAAGTGACT GGTTGGCTTC AGTAGAGTCA 
TTGCTTCCTA TTACTACTGT GATCCCTGTG CCTGCCTTTC TTCTGCTGGC TCACCTCCTT 
GTTGAAGACA AAGGACAAAA TCTTCACCAA ATACTCAAGG TCACTACAGA ATTAGCCCAA 
GCAGATTCCT CCCAGGTGCC AAATCTGATT CCAGTTTTGA TGTTCAAATT GGGAAGACCA 
CTGGAACCTA TATTATATAA TGATATATTG TATACTTTAC CTAAGCTTGG TGTTCACAAG 
GTGTGTATAG GACAAATTCT ACGAGTAATA CAACTGCTTG GAACCACACC ACGACTAAGA 
GCTGTCACTT TGCGCTTGCT GACATCTTTG TGGGAAAAGC AGGACCGAGT CTATCCTGAA 
CTGCAGCGTT TCATGGCTGT GTCTGATGTA CCTTCTCTTT CGGTGGGCAA GGAAGTCCAA 
TGGGAGAAAC TGATTGCAAA AGCAGCATCA ATCAGAGATA TATGTAAGCA GAGGCCATAT 
CAACATGGTG CAGATATGCT GGCAGCTATT TCTCAGGTGT TGAATGAATG CACCAAGCCT 
GATCAAGCTA CTCCAGCAGC CTTGGTATTA CAAGGTCTTC ATGCACTCTG TCAAGCTGAG 
GTTGTTTGCA TTCGCTCCAC TTGGAATGCT CTCTCTCCAA AGCTGAGTTG TGACACAAGA 
CCTCTCATTC TGAAGACACT GAGTGAACTA TTTTCTCTAG TTCCTTCCTT AACAGTCAAT 
ACAACTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAC TCATACTCAA 
AACAAGGACC CAATTGTGGC AAATGCTGCA TATAGATCCC TGGCCAATTT TAGTGCAGGA 
GAACACACCA TTCTTCATCT GCCTGAAAAG ATAAGACCAG AAATTCCCAT TCCTGAAGAG 
TTAGATGATG ATGAAGATGA TGAGGATGTG GATCTTTCAG TTCCTGGCTC TTGCTATCTC 
AAACTGTTGT CACTCACTCC CCCTTTGGTT TTACCAGCTT TGGAGGAATT TTTTACATCA 
CTTGTGAAGC AAGAAATGGT GAATATGCCT CGTGGGATAT ATCACTCTGC ATTAAAAGGA 
GGTGTCCGCT CAGACCAAGG AAAGACTGTA GCAGGAATCC CCAATTTTAT ATTGAAAATG 
TATGAAACAA ACAAGCAACC AGGATTGAAA CCTGGCCTTG CAGGTGGTAT GTTATTTTGC 
TATGATGTTT CCATGTATCA GAGTAAAGAT GGAAAACCAT TGAATAGACT GATGGCCAGC 
AGAGGGCGAA GTTTCAAGCA GACATCACTT GCTCTTGTAC ATGAGGTTCA TATCCAGCTT 
TCAGAGTGGC ACCGTGCGAT TTTTCTTCCA CAGGCCTGGC TTGCATACAT GAATCGAGCT 
TATCATGCCA TTTTACAGGG AAGACTAGGA GAGCTGGAGT TGCAGTTAAA ACATGGAAAA 
GAAGAACCTG AGGAGGTGCA GTACAAAAAA AGCACAGCTT GGCTCTGGGT TAGAGACATG 
CTGACTGATG AGATCACCAA GGCAGCTGCA AAGGAGAGTC CGGTAGTGAA AGGCAATGCG 
CTGTTAGCTC TAAGCAGCCT TGCTGTCGTC GTATCTAGAC ATGAAGCCAG CGTCTCCTCA 
GACTCTGACG GGCTCCTGGA GGTTCAACCT AATTTCCTTT CAATGAAAGA GTGGGTTTCC 
ATGATACTTG ATACACTCTT GGTCATTGTG GATAGCCATT ACCAACCCAG AGGGCAACTT 
CTCTCCTGGT TTTATTATAA GTCCTATTCT GGTGAAAACA CAGCTAGTGC CATTGCCCGT 
TCTGCTGCCG CCACGGCTTT GTCTCTCCTT GTGCCAGTTT TCATTATCTC TTGCAAAGAG 
AAGGTTGAGG AAATCCTGAA CATGCTGACT GCCAGGTTAC CTGGGAAACC AAGTGCTGAT 
GAGTCTCAAG CCGTGCAAAT CCACATGGGC CTTGCTTTAG GGATGTTTCT CTCTCGCTTG 
TGTGAAGAGA AACTCAGTGA TATATGTGGC CAAGAGATGA ACCTTCTTCT GATGAAGTCG 
TTGGATGCCC TGGAAAATTG CTGCTTTGAC ACTAGTCTTG AATACAACAC GGGCTGTATA 
TTGGGAGTTG GACTTGTTCT GTCCCTCATG AGTCACAGCA GCCAAATGCA GTCCTGCGTT 
CACGTGACAG CATTGCTTCG GAAGCTGTCT GCATACCTAG ATGACAGTGG GAGCCAGAGC 
AGAACGTTTC AGGAGGTCCT TGCCTACACA CTTAGCTGTG TATGTACATC AGCGTTCAGT 
GCTGGAATTA TTGAGGCTAC AGAGGCTGAG GATGTTATGA ACAAGCTTCG ACTGTTAGTG 
GAGAATAGCC AGCAGACTTC AGGTTTTGCC CTGGCTTTAG GAAACGTAGT TCATGGATTG 
TCTGTGTGTG GACATGGAAA AGCTGAAGAC TTGGGCAGCA AACTACTCCC TGCCTGGATC 
AGAATTGTTC TAACAGAGGG CACTCCCACA ATGCTTTGTC TGGCAGCTCT TCATGGCATG 
GTGGCCCTGG TAGGCTCTGA AGGGGATGTA ATGCAGCTGA AATCAGAAGC CATCCAGACC 
TCTCATTTTC AAGGCAGACT TAATGAAGTC ATTAGAACCT TAACTCAGGT CATTAGTGTC 
TCTGGGGTGA TTGGTCTCCA GTCAAATGCA GTCTGGCTTC TTGGACATCT TCATCTATCT 
ACTCTATCCT CAAGTCAAAG TAGAGCCTCT GTTCCTACTG ACTATAGCTA TTTGCCTGAA 
AGCAGTTTTA TTGGAGCAGC TATTGGCTTC TTCATTACAG GAGGAAAAAA AGGTCCTGAA 
TCTGTGCCTC CTTCCCTTCT TAAAGTAGTG ATGAAACCCA TAGCAACTGT TGGAGAAAGC 
TACCAATATC CTCCTGTGAA CTGGGCTGCA CTTCTCTCTC CACTTATGAG GCTAAATTTT 
GGTGAAGAGA TCCAGCAACT GTGCCTTGAA ATTATGGTGA CCCAGGCACA GTCATCCCAG 
AATGCAGCTG CACTATTGGG CTTGTGGGTG ACACCACCAC TGATCCACAG TCTGAGTCTG 
AATACCAAGA GATATCTCCT GATATCTGCA CCTCTGTGGA TAAAACACAT CTCTGATGAA 
CAGATCCTGG GTTTTGTTGA AAATTTAATG GTGGCAGTTT TTAAAGCAGC TTCCCCACTT 
GGAAATCCTG AGCTATGCCC GAGTGCTTTA CAGGGTCTGA GCCAGGCCAT GAAACTGCCC 
AGCCCTGCTC ACCACCTCTG GAGTCTGCTC TCTGAAGCTA CTGGGAAAAT TTTTGACCTC 
CTGCCAAATA AGATTCGGAG AAAGGATCTA GAGCTGTATA TCAGCATAGC AAAATGCCTC 
TTAGAAATGA CAGATGATGA TGCCAATCGG ATCGCCCAGG TTACTAAGAG CAACATAGAA 
AAGGCTGCCT TTGTCAAACT GTACTTAGTC TCTCAAGGAC GATTCCCCTT GGTGAACCTG 
ACCGATATGC TGAGTGTTGC TGTGCAGCAC CGTGAGAAAG AGGTGTTGGC CTGGATGATT 
CTGCACAGCT TATACCAGGC ACGGATTGTG AGCCATGCCA ACACGGGCGT TTTGAAGAGA 
ATGGAGTGGC TCTTGGAACT GATGGGTTAT ATTAGAAATG TTGCGTACCA GTCAACATCC 
TTTCAGAATA CGGCTCTTGA TGAGGCTTTG GACTTCTTCT TGCTGATATT TGCAACCGCA 
GTGGTTGCAT GGGCCGACCA CACTGCCCCT CTCCTCCTTG GTCTCAGTGC CAGTTGGTTG 
CCATGGCATC AGGAGAATGG CCCGGCTGGG CCAGTACCAA GCTTCCTTGG CAGGAGTCCA 
ATGCACAGGG TCACTGTGCA GGAGGTTCTC ACTCTCCTTC CCAATAGCAT GGCTCTGCTG 
CTGCAGAAAG AGCCATGGAA GGAAGAGACC CAGAAGTTCA TTGACTGGCT ATTCAGCATC 
ATGGAAAGCC CTAAAGAAGC CCTCTCAGCA AAGTCCAGGG ATCTTTTGAA AGCCACCCTG 
CTGTCCTTGA GAGTTCTCCC AGAGTTTAAG AAGAAAGCTG TATGGACCAG AGCGTATGGT 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009242667.2
CDS	97..5502
Translation	MSDDIRKRFEFPNSLIQSQAAGHLIAAVLKENGFSEKIYQSTNQTPALNLLWEKCCSDNVVVRTACCEGLVALVAQDHAEFSYVLNGILNLIPSTRNTHGLIKAIMKLLQMQALKEGQGGEKNIQSVYTIRNHPHPLITVLEHRPDCWPVFLQQLTAFFQQCPERSEVSCIQIMAPFLWYLYCEPSQLQEYAKLRLALLKVLLQPQVLCDKDQPSILQQQILQLCCDIVPCLQIKDLIQTTEAMMFIEEIYLSLLRHPVFWKIQLTQMTLQLLCVGEVSLKITGECSSSIHLLEHSVELLKEDFPVELVIIGIALLLLQTPASQQKPILNLALKLFSVTEDQKIPKSSLLLVMPILQILSSTALEDCISVDEEGPSRQQLALNLLEMIQQECYRDDHQKLSYKLVCPVTSMYGSVFTAWRILEVMTDSSAASDWLASVESLLPITTVIPVPAFLLLAHLLVEDKGQNLHQILKVTTELAQADSSQVPNLIPVLMFKLGRPLEPILYNDILYTLPKLGVHKVCIGQILRVIQLLGTTPRLRAVTLRLLTSLWEKQDRVYPELQRFMAVSDVPSLSVGKEVQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQATPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDTRPLILKTLSELFSLVPSLTVNTTEYENFKVQVLSFLWTHTQNKDPIVANAAYRSLANFSAGEHTILHLPEKIRPEIPIPEELDDDEDDEDVDLSVPGSCYLKLLSLTPPLVLPALEEFFTSLVKQEMVNMPRGIYHSALKGGVRSDQGKTVAGIPNFILKMYETNKQPGLKPGLAGGMLFCYDVSMYQSKDGKPLNRLMASRGRSFKQTSLALVHEVHIQLSEWHRAIFLPQAWLAYMNRAYHAILQGRLGELELQLKHGKEEPEEVQYKKSTAWLWVRDMLTDEITKAAAKESPVVKGNALLALSSLAVVVSRHEASVSSDSDGLLEVQPNFLSMKEWVSMILDTLLVIVDSHYQPRGQLLSWFYYKSYSGENTASAIARSAAATALSLLVPVFIISCKEKVEEILNMLTARLPGKPSADESQAVQIHMGLALGMFLSRLCEEKLSDICGQEMNLLLMKSLDALENCCFDTSLEYNTGCILGVGLVLSLMSHSSQMQSCVHVTALLRKLSAYLDDSGSQSRTFQEVLAYTLSCVCTSAFSAGIIEATEAEDVMNKLRLLVENSQQTSGFALALGNVVHGLSVCGHGKAEDLGSKLLPAWIRIVLTEGTPTMLCLAALHGMVALVGSEGDVMQLKSEAIQTSHFQGRLNEVIRTLTQVISVSGVIGLQSNAVWLLGHLHLSTLSSSQSRASVPTDYSYLPESSFIGAAIGFFITGGKKGPESVPPSLLKVVMKPIATVGESYQYPPVNWAALLSPLMRLNFGEEIQQLCLEIMVTQAQSSQNAAALLGLWVTPPLIHSLSLNTKRYLLISAPLWIKHISDEQILGFVENLMVAVFKAASPLGNPELCPSALQGLSQAMKLPSPAHHLWSLLSEATGKIFDLLPNKIRRKDLELYISIAKCLLEMTDDDANRIAQVTKSNIEKAAFVKLYLVSQGRFPLVNLTDMLSVAVQHREKEVLAWMILHSLYQARIVSHANTGVLKRMEWLLELMGYIRNVAYQSTSFQNTALDEALDFFLLIFATAVVAWADHTAPLLLGLSASWLPWHQENGPAGPVPSFLGRSPMHRVTVQEVLTLLPNSMALLLQKEPWKEETQKFIDWLFSIMESPKEALSAKSRDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_009244392.2
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii focadhesin (FOCAD), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009244392.2

ORF Insert Sequence:

ATGTCAGATG ATATCAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA ATCACAGGCT 
GCGGGTCATC TTATTGCTGC AGTCCTAAAG GAGAATGGTT TTTCAGAAAA GATTTACCAA 
TCTACAAATC AGACTCCTGC TTTGAACTTG CTGTGGGAGA AGTGTTGCAG TGACAATGTA 
GTGGTTCGAA CAGCCTGCTG TGAAGGTCTG GTGGCACTCG TTGCTCAGGA TCATGCAGAG 
TTCAGCTATG TTCTCAATGG GATACTCAAC TTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCATTATGAA ATTACTACAA ATGCAAGCTC TTAAGGAAGG ACAAGGTGGG 
GAAAAGAATA TTCAGAGTGT ATATACCATT AGAAATCATC CTCATCCTTT GATCACTGTG 
CTTGAACACA GACCTGATTG TTGGCCAGTG TTTTTGCAGC AGCTGACAGC ATTTTTCCAG 
CAGTGCCCTG AAAGGTCAGA AGTTTCATGC ATTCAAATAA TGGCACCATT TCTGTGGTAT 
CTGTATTGTG AACCATCTCA GTTACAAGAA TATGCTAAAC TCCGACTAGC CCTGCTGAAA 
GTCTTACTTC AACCCCAGGT TCTTTGTGAC AAGGATCAAC CATCAATACT GCAACAGCAG 
ATACTTCAAC TGTGTTGTGA CATAGTTCCA TGTTTGCAGA TAAAAGATTT GATACAGACA 
ACAGAAGCGA TGATGTTTAT TGAGGAAATA TATTTAAGCC TTTTGCGTCA TCCTGTTTTC 
TGGAAAATTC AGCTTACCCA GATGACTCTT CAGCTGCTGT GTGTCGGTGA AGTCAGCTTA 
AAGATAACTG GTGAATGTTC ATCTTCAATT CACCTCTTAG AGCACAGTGT TGAACTTCTG 
AAGGAGGATT TTCCTGTTGA ACTGGTCATA ATTGGAATAG CTTTACTACT TCTACAGACT 
CCAGCAAGTC AGCAGAAGCC AATCTTAAAT CTAGCTTTGA AGCTCTTCTC TGTTACTGAG 
GATCAGAAAA TCCCAAAGTC CTCTCTGCTG CTAGTGATGC CAATTCTGCA GATACTGTCT 
TCTACTGCCT TGGAAGACTG TATATCTGTG GATGAAGAAG GTCCCTCTAG GCAGCAGTTG 
GCTCTAAACC TTTTGGAAAT GATACAGCAG GAATGTTACA GAGATGACCA CCAAAAGCTC 
TCCTACAAGC TTGTGTGCCC TGTAACCAGT ATGTATGGTT CAGTATTTAC AGCCTGGAGG 
ATTCTTGAAG TAATGACAGA CTCCTCTGCT GCAAGTGACT GGTTGGCTTC AGTAGAGTCA 
TTGCTTCCTA TTACTACTGT GATCCCTGTG CCTGCCTTTC TTCTGCTGGC TCACCTCCTT 
GTTGAAGACA AAGGACAAAA TCTTCACCAA ATACTCAAGG TCACTACAGA ATTAGCCCAA 
GCAGATTCCT CCCAGGTGCC AAATCTGATT CCAGTTTTGA TGTTCAAATT GGGAAGACCA 
CTGGAACCTA TATTATATAA TGATATATTG TATACTTTAC CTAAGCTTGG TGTTCACAAG 
GTGTGTATAG GACAAATTCT ACGAGTAATA CAACTGCTTG GAACCACACC ACGACTAAGA 
GCTGTCACTT TGCGCTTGCT GACATCTTTG TGGGAAAAGC AGGACCGAGT CTATCCTGAA 
CTGCAGCGTT TCATGGCTGT GTCTGATGTA CCTTCTCTTT CGGTGGGCAA GGAAGTCCAA 
TGGGAGAAAC TGATTGCAAA AGCAGCATCA ATCAGAGATA TATGTAAGCA GAGGCCATAT 
CAACATGGTG CAGATATGCT GGCAGCTATT TCTCAGGTGT TGAATGAATG CACCAAGCCT 
GATCAAGCTA CTCCAGCAGC CTTGGTATTA CAAGGTCTTC ATGCACTCTG TCAAGCTGAG 
GTTGTTTGCA TTCGCTCCAC TTGGAATGCT CTCTCTCCAA AGCTGAGTTG TGACACAAGA 
CCTCTCATTC TGAAGACACT GAGTGAACTA TTTTCTCTAG TTCCTTCCTT AACAGTCAAT 
ACAACTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAC TCATACTCAA 
AACAAGGACC CAATTGTGGC AAATGCTGCA TATAGATCCC TGGCCAATTT TAGTGCAGGA 
GAACACACCA TTCTTCATCT GCCTGAAAAG ATAAGACCAG AAATTCCCAT TCCTGAAGAG 
TTAGATGATG ATGAAGATGA TGAGGATGTG GATCTTTCAG TTCCTGGCTC TTGCTATCTC 
AAACTGTTGT CACTCACTCC CCCTTTGGTT TTACCAGCTT TGGAGGAATT TTTTACATCA 
CTTGTGAAGC AAGAAATGGT GAATATGCCT CGTGGGATAT ATCACTCTGC ATTAAAAGGA 
GGTGTCCGCT CAGACCAAGG AAAGACTGTA GCAGGAATCC CCAATTTTAT ATTGAAAATG 
TATGAAACAA ACAAGCAACC AGGATTGAAA CCTGGCCTTG CAGGTGGTAT GTTATTTTGC 
TATGATGTTT CCATGTATCA GAGTAAAGAT GGAAAACCAT TGAATAGACT GATGGCCAGC 
AGAGGGCGAA GTTTCAAGCA GACATCACTT GCTCTTGTAC ATGAGGTTCA TATCCAGCTT 
TCAGAGTGGC ACCGTGCGAT TTTTCTTCCA CAGGCCTGGC TTGCATACAT GAATCGAGCT 
TATCATGCCA TTTTACAGGG AAGACTAGGA GAGCTGGAGT TGCAGTTAAA ACATGGAAAA 
GAAGAACCTG AGGAGGTGCA GTACAAAAAA AGCACAGCTT GGCTCTGGGT TAGAGACATG 
CTGACTGATG AGATCACCAA GGCAGCTGCA AAGGAGAGTC CGGTAGTGAA AGGCAATGCG 
CTGTTAGCTC TAAGCAGCCT TGCTGTCGTC GTATCTAGAC ATGAAGCCAG CGTCTCCTCA 
GACTCTGACG GGCTCCTGGA GGTTCAACCT AATTTCCTTT CAATGAAAGA GTGGGTTTCC 
ATGATACTTG ATACACTCTT GGTCATTGTG GATAGCCATT ACCAACCCAG AGGGCAACTT 
CTCTCCTGGT TTTATTATAA GTCCTATTCT GGTGAAAACA CAGCTAGTGC CATTGCCCGT 
TCTGCTGCCG CCACGGCTTT GTCTCTCCTT GTGCCAGTTT TCATTATCTC TTGCAAAGAG 
AAGGTTGAGG AAATCCTGAA CATGCTGACT GCCAGGTTAC CTGGGAAACC AAGTGCTGAT 
GAGTCTCAAG CCGTGCAAAT CCACATGGGC CTTGCTTTAG GGATGTTTCT CTCTCGCTTG 
TGTGAAGAGA AACTCAGTGA TATATGTGGC CAAGAGATGA ACCTTCTTCT GATGAAGTCG 
TTGGATGCCC TGGAAAATTG CTGCTTTGAC ACTAGTCTTG AATACAACAC GGGCTGTATA 
TTGGGAGTTG GACTTGTTCT GTCCCTCATG AGTCACAGCA GCCAAATGCA GTCCTGCGTT 
CACGTGACAG CATTGCTTCG GAAGCTGTCT GCATACCTAG ATGACAGTGG GAGCCAGAGC 
AGAACGTTTC AGGAGGTCCT TGCCTACACA CTTAGCTGTG TATGTACATC AGCGTTCAGT 
GCTGGAATTA TTGAGGCTAC AGAGGCTGAG GATGTTATGA ACAAGCTTCG ACTGTTAGTG 
GAGAATAGCC AGCAGACTTC AGGTTTTGCC CTGGCTTTAG GAAACGTAGT TCATGGATTG 
TCTGTGTGTG GACATGGAAA AGCTGAAGAC TTGGGCAGCA AACTACTCCC TGCCTGGATC 
AGAATTGTTC TAACAGAGGG CACTCCCACA ATGCTTTGTC TGGCAGCTCT TCATGGCATG 
GTGGCCCTGG TAGGCTCTGA AGGGGATGTA ATGCAGCTGA AATCAGAAGC CATCCAGACC 
TCTCATTTTC AAGGCAGACT TAATGAAGTC ATTAGAACCT TAACTCAGGT CATTAGTGTC 
TCTGGGGTGA TTGGTCTCCA GTCAAATGCA GTCTGGCTTC TTGGACATCT TCATCTATCT 
ACTCTATCCT CAAGTCAAAG TAGAGCCTCT GTTCCTACTG ACTATAGCTA TTTGCCTGAA 
AGCAGTTTTA TTGGAGCAGC TATTGGCTTC TTCATTACAG GAGGAAAAAA AGGTCCTGAA 
TCTGTGCCTC CTTCCCTTCT TAAAGTAGTG ATGAAACCCA TAGCAACTGT TGGAGAAAGC 
TACCAATATC CTCCTGTGAA CTGGGCTGCA CTTCTCTCTC CACTTATGAG GCTAAATTTT 
GGTGAAGAGA TCCAGCAACT GTGCCTTGAA ATTATGGTGA CCCAGGCACA GTCATCCCAG 
AATGCAGCTG CACTATTGGG CTTGTGGGTG ACACCACCAC TGATCCACAG TCTGAGTCTG 
AATACCAAGA GATATCTCCT GATATCTGCA CCTCTGTGGA TAAAACACAT CTCTGATGAA 
CAGATCCTGG GTTTTGTTGA AAATTTAATG GTGGCAGTTT TTAAAGCAGC TTCCCCACTT 
GGAAATCCTG AGCTATGCCC GAGTGCTTTA CAGGGTCTGA GCCAGGCCAT GAAACTGCCC 
AGCCCTGCTC ACCACCTCTG GAGTCTGCTC TCTGAAGCTA CTGGGAAAAT TTTTGACCTC 
CTGCCAAATA AGATTCGGAG AAAGGATCTA GAGCTGTATA TCAGCATAGC AAAATGCCTC 
TTAGAAATGA CAGATGATGA TGCCAATCGG ATCGCCCAGG TTACTAAGAG CAACATAGAA 
AAGGCTGCCT TTGTCAAACT GTACTTAGTC TCTCAAGGAC GATTCCCCTT GGTGAACCTG 
ACCGATATGC TGAGTGTTGC TGTGCAGCAC CGTGAGAAAG AGGTGTTGGC CTGGATGATT 
CTGCACAGCT TATACCAGGC ACGGATTGTG AGCCATGCCA ACACGGGCGT TTTGAAGAGA 
ATGGAGTGGC TCTTGGAACT GATGGGTTAT ATTAGAAATG TTGCGTACCA GTCAACATCC 
TTTCAGAATA CGGCTCTTGA TGAGGCTTTG GACTTCTTCT TGCTGATATT TGCAACCGCA 
GTGGTTGCAT GGGCCGACCA CACTGCCCCT CTCCTCCTTG GTCTCAGTGC CAGTTGGTTG 
CCATGGCATC AGGAGAATGG CCCGGCTGGG CCAGTACCAA GCTTCCTTGG CAGGAGTCCA 
ATGCACAGGG TCACTGTGCA GGAGGTTCTC ACTCTCCTTC CCAATAGCAT GGCTCTGCTG 
CTGCAGAAAG AGCCATGGAA GGAAGAGACC CAGAAGTTCA TTGACTGGCT ATTCAGCATC 
ATGGAAAGCC CTAAAGAAGC CCTCTCAGCA AAGTCCAGGG ATCTTTTGAA AGCCACCCTG 
CTGTCCTTGA GAGTTCTCCC AGAGTTTAAG AAGAAAGCTG TATGGACCAG AGCGTATGGT 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FOCAD ( XM_024252331.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024108099.1 Pongo abelii focadhesin (FOCAD), transcript variant X6, mRNA.

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CloneID	OSa186756
Clone ID Related Accession (Same CDS sequence)	XM_009244392.2 , XM_024252331.1 , XM_024252332.1 , XM_024252334.1 , XM_009244394.2
Accession Version	XM_024252331.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	focadhesin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036912.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGATG ATATCAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA ATCACAGGCT 
GCGGGTCATC TTATTGCTGC AGTCCTAAAG GAGAATGGTT TTTCAGAAAA GATTTACCAA 
TCTACAAATC AGACTCCTGC TTTGAACTTG CTGTGGGAGA AGTGTTGCAG TGACAATGTA 
GTGGTTCGAA CAGCCTGCTG TGAAGGTCTG GTGGCACTCG TTGCTCAGGA TCATGCAGAG 
TTCAGCTATG TTCTCAATGG GATACTCAAC TTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCATTATGAA ATTACTACAA ATGCAAGCTC TTAAGGAAGG ACAAGGTGGG 
GAAAAGAATA TTCAGAGTGT ATATACCATT AGAAATCATC CTCATCCTTT GATCACTGTG 
CTTGAACACA GACCTGATTG TTGGCCAGTG TTTTTGCAGC AGCTGACAGC ATTTTTCCAG 
CAGTGCCCTG AAAGGTCAGA AGTTTCATGC ATTCAAATAA TGGCACCATT TCTGTGGTAT 
CTGTATTGTG AACCATCTCA GTTACAAGAA TATGCTAAAC TCCGACTAGC CCTGCTGAAA 
GTCTTACTTC AACCCCAGGT TCTTTGTGAC AAGGATCAAC CATCAATACT GCAACAGCAG 
ATACTTCAAC TGTGTTGTGA CATAGTTCCA TGTTTGCAGA TAAAAGATTT GATACAGACA 
ACAGAAGCGA TGATGTTTAT TGAGGAAATA TATTTAAGCC TTTTGCGTCA TCCTGTTTTC 
TGGAAAATTC AGCTTACCCA GATGACTCTT CAGCTGCTGT GTGTCGGTGA AGTCAGCTTA 
AAGATAACTG GTGAATGTTC ATCTTCAATT CACCTCTTAG AGCACAGTGT TGAACTTCTG 
AAGGAGGATT TTCCTGTTGA ACTGGTCATA ATTGGAATAG CTTTACTACT TCTACAGACT 
CCAGCAAGTC AGCAGAAGCC AATCTTAAAT CTAGCTTTGA AGCTCTTCTC TGTTACTGAG 
GATCAGAAAA TCCCAAAGTC CTCTCTGCTG CTAGTGATGC CAATTCTGCA GATACTGTCT 
TCTACTGCCT TGGAAGACTG TATATCTGTG GATGAAGAAG GTCCCTCTAG GCAGCAGTTG 
GCTCTAAACC TTTTGGAAAT GATACAGCAG GAATGTTACA GAGATGACCA CCAAAAGCTC 
TCCTACAAGC TTGTGTGCCC TGTAACCAGT ATGTATGGTT CAGTATTTAC AGCCTGGAGG 
ATTCTTGAAG TAATGACAGA CTCCTCTGCT GCAAGTGACT GGTTGGCTTC AGTAGAGTCA 
TTGCTTCCTA TTACTACTGT GATCCCTGTG CCTGCCTTTC TTCTGCTGGC TCACCTCCTT 
GTTGAAGACA AAGGACAAAA TCTTCACCAA ATACTCAAGG TCACTACAGA ATTAGCCCAA 
GCAGATTCCT CCCAGGTGCC AAATCTGATT CCAGTTTTGA TGTTCAAATT GGGAAGACCA 
CTGGAACCTA TATTATATAA TGATATATTG TATACTTTAC CTAAGCTTGG TGTTCACAAG 
GTGTGTATAG GACAAATTCT ACGAGTAATA CAACTGCTTG GAACCACACC ACGACTAAGA 
GCTGTCACTT TGCGCTTGCT GACATCTTTG TGGGAAAAGC AGGACCGAGT CTATCCTGAA 
CTGCAGCGTT TCATGGCTGT GTCTGATGTA CCTTCTCTTT CGGTGGGCAA GGAAGTCCAA 
TGGGAGAAAC TGATTGCAAA AGCAGCATCA ATCAGAGATA TATGTAAGCA GAGGCCATAT 
CAACATGGTG CAGATATGCT GGCAGCTATT TCTCAGGTGT TGAATGAATG CACCAAGCCT 
GATCAAGCTA CTCCAGCAGC CTTGGTATTA CAAGGTCTTC ATGCACTCTG TCAAGCTGAG 
GTTGTTTGCA TTCGCTCCAC TTGGAATGCT CTCTCTCCAA AGCTGAGTTG TGACACAAGA 
CCTCTCATTC TGAAGACACT GAGTGAACTA TTTTCTCTAG TTCCTTCCTT AACAGTCAAT 
ACAACTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAC TCATACTCAA 
AACAAGGACC CAATTGTGGC AAATGCTGCA TATAGATCCC TGGCCAATTT TAGTGCAGGA 
GAACACACCA TTCTTCATCT GCCTGAAAAG ATAAGACCAG AAATTCCCAT TCCTGAAGAG 
TTAGATGATG ATGAAGATGA TGAGGATGTG GATCTTTCAG TTCCTGGCTC TTGCTATCTC 
AAACTGTTGT CACTCACTCC CCCTTTGGTT TTACCAGCTT TGGAGGAATT TTTTACATCA 
CTTGTGAAGC AAGAAATGGT GAATATGCCT CGTGGGATAT ATCACTCTGC ATTAAAAGGA 
GGTGTCCGCT CAGACCAAGG AAAGACTGTA GCAGGAATCC CCAATTTTAT ATTGAAAATG 
TATGAAACAA ACAAGCAACC AGGATTGAAA CCTGGCCTTG CAGGTGGTAT GTTATTTTGC 
TATGATGTTT CCATGTATCA GAGTAAAGAT GGAAAACCAT TGAATAGACT GATGGCCAGC 
AGAGGGCGAA GTTTCAAGCA GACATCACTT GCTCTTGTAC ATGAGGTTCA TATCCAGCTT 
TCAGAGTGGC ACCGTGCGAT TTTTCTTCCA CAGGCCTGGC TTGCATACAT GAATCGAGCT 
TATCATGCCA TTTTACAGGG AAGACTAGGA GAGCTGGAGT TGCAGTTAAA ACATGGAAAA 
GAAGAACCTG AGGAGGTGCA GTACAAAAAA AGCACAGCTT GGCTCTGGGT TAGAGACATG 
CTGACTGATG AGATCACCAA GGCAGCTGCA AAGGAGAGTC CGGTAGTGAA AGGCAATGCG 
CTGTTAGCTC TAAGCAGCCT TGCTGTCGTC GTATCTAGAC ATGAAGCCAG CGTCTCCTCA 
GACTCTGACG GGCTCCTGGA GGTTCAACCT AATTTCCTTT CAATGAAAGA GTGGGTTTCC 
ATGATACTTG ATACACTCTT GGTCATTGTG GATAGCCATT ACCAACCCAG AGGGCAACTT 
CTCTCCTGGT TTTATTATAA GTCCTATTCT GGTGAAAACA CAGCTAGTGC CATTGCCCGT 
TCTGCTGCCG CCACGGCTTT GTCTCTCCTT GTGCCAGTTT TCATTATCTC TTGCAAAGAG 
AAGGTTGAGG AAATCCTGAA CATGCTGACT GCCAGGTTAC CTGGGAAACC AAGTGCTGAT 
GAGTCTCAAG CCGTGCAAAT CCACATGGGC CTTGCTTTAG GGATGTTTCT CTCTCGCTTG 
TGTGAAGAGA AACTCAGTGA TATATGTGGC CAAGAGATGA ACCTTCTTCT GATGAAGTCG 
TTGGATGCCC TGGAAAATTG CTGCTTTGAC ACTAGTCTTG AATACAACAC GGGCTGTATA 
TTGGGAGTTG GACTTGTTCT GTCCCTCATG AGTCACAGCA GCCAAATGCA GTCCTGCGTT 
CACGTGACAG CATTGCTTCG GAAGCTGTCT GCATACCTAG ATGACAGTGG GAGCCAGAGC 
AGAACGTTTC AGGAGGTCCT TGCCTACACA CTTAGCTGTG TATGTACATC AGCGTTCAGT 
GCTGGAATTA TTGAGGCTAC AGAGGCTGAG GATGTTATGA ACAAGCTTCG ACTGTTAGTG 
GAGAATAGCC AGCAGACTTC AGGTTTTGCC CTGGCTTTAG GAAACGTAGT TCATGGATTG 
TCTGTGTGTG GACATGGAAA AGCTGAAGAC TTGGGCAGCA AACTACTCCC TGCCTGGATC 
AGAATTGTTC TAACAGAGGG CACTCCCACA ATGCTTTGTC TGGCAGCTCT TCATGGCATG 
GTGGCCCTGG TAGGCTCTGA AGGGGATGTA ATGCAGCTGA AATCAGAAGC CATCCAGACC 
TCTCATTTTC AAGGCAGACT TAATGAAGTC ATTAGAACCT TAACTCAGGT CATTAGTGTC 
TCTGGGGTGA TTGGTCTCCA GTCAAATGCA GTCTGGCTTC TTGGACATCT TCATCTATCT 
ACTCTATCCT CAAGTCAAAG TAGAGCCTCT GTTCCTACTG ACTATAGCTA TTTGCCTGAA 
AGCAGTTTTA TTGGAGCAGC TATTGGCTTC TTCATTACAG GAGGAAAAAA AGGTCCTGAA 
TCTGTGCCTC CTTCCCTTCT TAAAGTAGTG ATGAAACCCA TAGCAACTGT TGGAGAAAGC 
TACCAATATC CTCCTGTGAA CTGGGCTGCA CTTCTCTCTC CACTTATGAG GCTAAATTTT 
GGTGAAGAGA TCCAGCAACT GTGCCTTGAA ATTATGGTGA CCCAGGCACA GTCATCCCAG 
AATGCAGCTG CACTATTGGG CTTGTGGGTG ACACCACCAC TGATCCACAG TCTGAGTCTG 
AATACCAAGA GATATCTCCT GATATCTGCA CCTCTGTGGA TAAAACACAT CTCTGATGAA 
CAGATCCTGG GTTTTGTTGA AAATTTAATG GTGGCAGTTT TTAAAGCAGC TTCCCCACTT 
GGAAATCCTG AGCTATGCCC GAGTGCTTTA CAGGGTCTGA GCCAGGCCAT GAAACTGCCC 
AGCCCTGCTC ACCACCTCTG GAGTCTGCTC TCTGAAGCTA CTGGGAAAAT TTTTGACCTC 
CTGCCAAATA AGATTCGGAG AAAGGATCTA GAGCTGTATA TCAGCATAGC AAAATGCCTC 
TTAGAAATGA CAGATGATGA TGCCAATCGG ATCGCCCAGG TTACTAAGAG CAACATAGAA 
AAGGCTGCCT TTGTCAAACT GTACTTAGTC TCTCAAGGAC GATTCCCCTT GGTGAACCTG 
ACCGATATGC TGAGTGTTGC TGTGCAGCAC CGTGAGAAAG AGGTGTTGGC CTGGATGATT 
CTGCACAGCT TATACCAGGC ACGGATTGTG AGCCATGCCA ACACGGGCGT TTTGAAGAGA 
ATGGAGTGGC TCTTGGAACT GATGGGTTAT ATTAGAAATG TTGCGTACCA GTCAACATCC 
TTTCAGAATA CGGCTCTTGA TGAGGCTTTG GACTTCTTCT TGCTGATATT TGCAACCGCA 
GTGGTTGCAT GGGCCGACCA CACTGCCCCT CTCCTCCTTG GTCTCAGTGC CAGTTGGTTG 
CCATGGCATC AGGAGAATGG CCCGGCTGGG CCAGTACCAA GCTTCCTTGG CAGGAGTCCA 
ATGCACAGGG TCACTGTGCA GGAGGTTCTC ACTCTCCTTC CCAATAGCAT GGCTCTGCTG 
CTGCAGAAAG AGCCATGGAA GGAAGAGACC CAGAAGTTCA TTGACTGGCT ATTCAGCATC 
ATGGAAAGCC CTAAAGAAGC CCTCTCAGCA AAGTCCAGGG ATCTTTTGAA AGCCACCCTG 
CTGTCCTTGA GAGTTCTCCC AGAGTTTAAG AAGAAAGCTG TATGGACCAG AGCGTATGGT 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024108099.1
CDS	172..5577
Translation	MSDDIRKRFEFPNSLIQSQAAGHLIAAVLKENGFSEKIYQSTNQTPALNLLWEKCCSDNVVVRTACCEGLVALVAQDHAEFSYVLNGILNLIPSTRNTHGLIKAIMKLLQMQALKEGQGGEKNIQSVYTIRNHPHPLITVLEHRPDCWPVFLQQLTAFFQQCPERSEVSCIQIMAPFLWYLYCEPSQLQEYAKLRLALLKVLLQPQVLCDKDQPSILQQQILQLCCDIVPCLQIKDLIQTTEAMMFIEEIYLSLLRHPVFWKIQLTQMTLQLLCVGEVSLKITGECSSSIHLLEHSVELLKEDFPVELVIIGIALLLLQTPASQQKPILNLALKLFSVTEDQKIPKSSLLLVMPILQILSSTALEDCISVDEEGPSRQQLALNLLEMIQQECYRDDHQKLSYKLVCPVTSMYGSVFTAWRILEVMTDSSAASDWLASVESLLPITTVIPVPAFLLLAHLLVEDKGQNLHQILKVTTELAQADSSQVPNLIPVLMFKLGRPLEPILYNDILYTLPKLGVHKVCIGQILRVIQLLGTTPRLRAVTLRLLTSLWEKQDRVYPELQRFMAVSDVPSLSVGKEVQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQATPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDTRPLILKTLSELFSLVPSLTVNTTEYENFKVQVLSFLWTHTQNKDPIVANAAYRSLANFSAGEHTILHLPEKIRPEIPIPEELDDDEDDEDVDLSVPGSCYLKLLSLTPPLVLPALEEFFTSLVKQEMVNMPRGIYHSALKGGVRSDQGKTVAGIPNFILKMYETNKQPGLKPGLAGGMLFCYDVSMYQSKDGKPLNRLMASRGRSFKQTSLALVHEVHIQLSEWHRAIFLPQAWLAYMNRAYHAILQGRLGELELQLKHGKEEPEEVQYKKSTAWLWVRDMLTDEITKAAAKESPVVKGNALLALSSLAVVVSRHEASVSSDSDGLLEVQPNFLSMKEWVSMILDTLLVIVDSHYQPRGQLLSWFYYKSYSGENTASAIARSAAATALSLLVPVFIISCKEKVEEILNMLTARLPGKPSADESQAVQIHMGLALGMFLSRLCEEKLSDICGQEMNLLLMKSLDALENCCFDTSLEYNTGCILGVGLVLSLMSHSSQMQSCVHVTALLRKLSAYLDDSGSQSRTFQEVLAYTLSCVCTSAFSAGIIEATEAEDVMNKLRLLVENSQQTSGFALALGNVVHGLSVCGHGKAEDLGSKLLPAWIRIVLTEGTPTMLCLAALHGMVALVGSEGDVMQLKSEAIQTSHFQGRLNEVIRTLTQVISVSGVIGLQSNAVWLLGHLHLSTLSSSQSRASVPTDYSYLPESSFIGAAIGFFITGGKKGPESVPPSLLKVVMKPIATVGESYQYPPVNWAALLSPLMRLNFGEEIQQLCLEIMVTQAQSSQNAAALLGLWVTPPLIHSLSLNTKRYLLISAPLWIKHISDEQILGFVENLMVAVFKAASPLGNPELCPSALQGLSQAMKLPSPAHHLWSLLSEATGKIFDLLPNKIRRKDLELYISIAKCLLEMTDDDANRIAQVTKSNIEKAAFVKLYLVSQGRFPLVNLTDMLSVAVQHREKEVLAWMILHSLYQARIVSHANTGVLKRMEWLLELMGYIRNVAYQSTSFQNTALDEALDFFLLIFATAVVAWADHTAPLLLGLSASWLPWHQENGPAGPVPSFLGRSPMHRVTVQEVLTLLPNSMALLLQKEPWKEETQKFIDWLFSIMESPKEALSAKSRDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_024252331.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii focadhesin (FOCAD), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024252331.1

ORF Insert Sequence:

ATGTCAGATG ATATCAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA ATCACAGGCT 
GCGGGTCATC TTATTGCTGC AGTCCTAAAG GAGAATGGTT TTTCAGAAAA GATTTACCAA 
TCTACAAATC AGACTCCTGC TTTGAACTTG CTGTGGGAGA AGTGTTGCAG TGACAATGTA 
GTGGTTCGAA CAGCCTGCTG TGAAGGTCTG GTGGCACTCG TTGCTCAGGA TCATGCAGAG 
TTCAGCTATG TTCTCAATGG GATACTCAAC TTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCATTATGAA ATTACTACAA ATGCAAGCTC TTAAGGAAGG ACAAGGTGGG 
GAAAAGAATA TTCAGAGTGT ATATACCATT AGAAATCATC CTCATCCTTT GATCACTGTG 
CTTGAACACA GACCTGATTG TTGGCCAGTG TTTTTGCAGC AGCTGACAGC ATTTTTCCAG 
CAGTGCCCTG AAAGGTCAGA AGTTTCATGC ATTCAAATAA TGGCACCATT TCTGTGGTAT 
CTGTATTGTG AACCATCTCA GTTACAAGAA TATGCTAAAC TCCGACTAGC CCTGCTGAAA 
GTCTTACTTC AACCCCAGGT TCTTTGTGAC AAGGATCAAC CATCAATACT GCAACAGCAG 
ATACTTCAAC TGTGTTGTGA CATAGTTCCA TGTTTGCAGA TAAAAGATTT GATACAGACA 
ACAGAAGCGA TGATGTTTAT TGAGGAAATA TATTTAAGCC TTTTGCGTCA TCCTGTTTTC 
TGGAAAATTC AGCTTACCCA GATGACTCTT CAGCTGCTGT GTGTCGGTGA AGTCAGCTTA 
AAGATAACTG GTGAATGTTC ATCTTCAATT CACCTCTTAG AGCACAGTGT TGAACTTCTG 
AAGGAGGATT TTCCTGTTGA ACTGGTCATA ATTGGAATAG CTTTACTACT TCTACAGACT 
CCAGCAAGTC AGCAGAAGCC AATCTTAAAT CTAGCTTTGA AGCTCTTCTC TGTTACTGAG 
GATCAGAAAA TCCCAAAGTC CTCTCTGCTG CTAGTGATGC CAATTCTGCA GATACTGTCT 
TCTACTGCCT TGGAAGACTG TATATCTGTG GATGAAGAAG GTCCCTCTAG GCAGCAGTTG 
GCTCTAAACC TTTTGGAAAT GATACAGCAG GAATGTTACA GAGATGACCA CCAAAAGCTC 
TCCTACAAGC TTGTGTGCCC TGTAACCAGT ATGTATGGTT CAGTATTTAC AGCCTGGAGG 
ATTCTTGAAG TAATGACAGA CTCCTCTGCT GCAAGTGACT GGTTGGCTTC AGTAGAGTCA 
TTGCTTCCTA TTACTACTGT GATCCCTGTG CCTGCCTTTC TTCTGCTGGC TCACCTCCTT 
GTTGAAGACA AAGGACAAAA TCTTCACCAA ATACTCAAGG TCACTACAGA ATTAGCCCAA 
GCAGATTCCT CCCAGGTGCC AAATCTGATT CCAGTTTTGA TGTTCAAATT GGGAAGACCA 
CTGGAACCTA TATTATATAA TGATATATTG TATACTTTAC CTAAGCTTGG TGTTCACAAG 
GTGTGTATAG GACAAATTCT ACGAGTAATA CAACTGCTTG GAACCACACC ACGACTAAGA 
GCTGTCACTT TGCGCTTGCT GACATCTTTG TGGGAAAAGC AGGACCGAGT CTATCCTGAA 
CTGCAGCGTT TCATGGCTGT GTCTGATGTA CCTTCTCTTT CGGTGGGCAA GGAAGTCCAA 
TGGGAGAAAC TGATTGCAAA AGCAGCATCA ATCAGAGATA TATGTAAGCA GAGGCCATAT 
CAACATGGTG CAGATATGCT GGCAGCTATT TCTCAGGTGT TGAATGAATG CACCAAGCCT 
GATCAAGCTA CTCCAGCAGC CTTGGTATTA CAAGGTCTTC ATGCACTCTG TCAAGCTGAG 
GTTGTTTGCA TTCGCTCCAC TTGGAATGCT CTCTCTCCAA AGCTGAGTTG TGACACAAGA 
CCTCTCATTC TGAAGACACT GAGTGAACTA TTTTCTCTAG TTCCTTCCTT AACAGTCAAT 
ACAACTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAC TCATACTCAA 
AACAAGGACC CAATTGTGGC AAATGCTGCA TATAGATCCC TGGCCAATTT TAGTGCAGGA 
GAACACACCA TTCTTCATCT GCCTGAAAAG ATAAGACCAG AAATTCCCAT TCCTGAAGAG 
TTAGATGATG ATGAAGATGA TGAGGATGTG GATCTTTCAG TTCCTGGCTC TTGCTATCTC 
AAACTGTTGT CACTCACTCC CCCTTTGGTT TTACCAGCTT TGGAGGAATT TTTTACATCA 
CTTGTGAAGC AAGAAATGGT GAATATGCCT CGTGGGATAT ATCACTCTGC ATTAAAAGGA 
GGTGTCCGCT CAGACCAAGG AAAGACTGTA GCAGGAATCC CCAATTTTAT ATTGAAAATG 
TATGAAACAA ACAAGCAACC AGGATTGAAA CCTGGCCTTG CAGGTGGTAT GTTATTTTGC 
TATGATGTTT CCATGTATCA GAGTAAAGAT GGAAAACCAT TGAATAGACT GATGGCCAGC 
AGAGGGCGAA GTTTCAAGCA GACATCACTT GCTCTTGTAC ATGAGGTTCA TATCCAGCTT 
TCAGAGTGGC ACCGTGCGAT TTTTCTTCCA CAGGCCTGGC TTGCATACAT GAATCGAGCT 
TATCATGCCA TTTTACAGGG AAGACTAGGA GAGCTGGAGT TGCAGTTAAA ACATGGAAAA 
GAAGAACCTG AGGAGGTGCA GTACAAAAAA AGCACAGCTT GGCTCTGGGT TAGAGACATG 
CTGACTGATG AGATCACCAA GGCAGCTGCA AAGGAGAGTC CGGTAGTGAA AGGCAATGCG 
CTGTTAGCTC TAAGCAGCCT TGCTGTCGTC GTATCTAGAC ATGAAGCCAG CGTCTCCTCA 
GACTCTGACG GGCTCCTGGA GGTTCAACCT AATTTCCTTT CAATGAAAGA GTGGGTTTCC 
ATGATACTTG ATACACTCTT GGTCATTGTG GATAGCCATT ACCAACCCAG AGGGCAACTT 
CTCTCCTGGT TTTATTATAA GTCCTATTCT GGTGAAAACA CAGCTAGTGC CATTGCCCGT 
TCTGCTGCCG CCACGGCTTT GTCTCTCCTT GTGCCAGTTT TCATTATCTC TTGCAAAGAG 
AAGGTTGAGG AAATCCTGAA CATGCTGACT GCCAGGTTAC CTGGGAAACC AAGTGCTGAT 
GAGTCTCAAG CCGTGCAAAT CCACATGGGC CTTGCTTTAG GGATGTTTCT CTCTCGCTTG 
TGTGAAGAGA AACTCAGTGA TATATGTGGC CAAGAGATGA ACCTTCTTCT GATGAAGTCG 
TTGGATGCCC TGGAAAATTG CTGCTTTGAC ACTAGTCTTG AATACAACAC GGGCTGTATA 
TTGGGAGTTG GACTTGTTCT GTCCCTCATG AGTCACAGCA GCCAAATGCA GTCCTGCGTT 
CACGTGACAG CATTGCTTCG GAAGCTGTCT GCATACCTAG ATGACAGTGG GAGCCAGAGC 
AGAACGTTTC AGGAGGTCCT TGCCTACACA CTTAGCTGTG TATGTACATC AGCGTTCAGT 
GCTGGAATTA TTGAGGCTAC AGAGGCTGAG GATGTTATGA ACAAGCTTCG ACTGTTAGTG 
GAGAATAGCC AGCAGACTTC AGGTTTTGCC CTGGCTTTAG GAAACGTAGT TCATGGATTG 
TCTGTGTGTG GACATGGAAA AGCTGAAGAC TTGGGCAGCA AACTACTCCC TGCCTGGATC 
AGAATTGTTC TAACAGAGGG CACTCCCACA ATGCTTTGTC TGGCAGCTCT TCATGGCATG 
GTGGCCCTGG TAGGCTCTGA AGGGGATGTA ATGCAGCTGA AATCAGAAGC CATCCAGACC 
TCTCATTTTC AAGGCAGACT TAATGAAGTC ATTAGAACCT TAACTCAGGT CATTAGTGTC 
TCTGGGGTGA TTGGTCTCCA GTCAAATGCA GTCTGGCTTC TTGGACATCT TCATCTATCT 
ACTCTATCCT CAAGTCAAAG TAGAGCCTCT GTTCCTACTG ACTATAGCTA TTTGCCTGAA 
AGCAGTTTTA TTGGAGCAGC TATTGGCTTC TTCATTACAG GAGGAAAAAA AGGTCCTGAA 
TCTGTGCCTC CTTCCCTTCT TAAAGTAGTG ATGAAACCCA TAGCAACTGT TGGAGAAAGC 
TACCAATATC CTCCTGTGAA CTGGGCTGCA CTTCTCTCTC CACTTATGAG GCTAAATTTT 
GGTGAAGAGA TCCAGCAACT GTGCCTTGAA ATTATGGTGA CCCAGGCACA GTCATCCCAG 
AATGCAGCTG CACTATTGGG CTTGTGGGTG ACACCACCAC TGATCCACAG TCTGAGTCTG 
AATACCAAGA GATATCTCCT GATATCTGCA CCTCTGTGGA TAAAACACAT CTCTGATGAA 
CAGATCCTGG GTTTTGTTGA AAATTTAATG GTGGCAGTTT TTAAAGCAGC TTCCCCACTT 
GGAAATCCTG AGCTATGCCC GAGTGCTTTA CAGGGTCTGA GCCAGGCCAT GAAACTGCCC 
AGCCCTGCTC ACCACCTCTG GAGTCTGCTC TCTGAAGCTA CTGGGAAAAT TTTTGACCTC 
CTGCCAAATA AGATTCGGAG AAAGGATCTA GAGCTGTATA TCAGCATAGC AAAATGCCTC 
TTAGAAATGA CAGATGATGA TGCCAATCGG ATCGCCCAGG TTACTAAGAG CAACATAGAA 
AAGGCTGCCT TTGTCAAACT GTACTTAGTC TCTCAAGGAC GATTCCCCTT GGTGAACCTG 
ACCGATATGC TGAGTGTTGC TGTGCAGCAC CGTGAGAAAG AGGTGTTGGC CTGGATGATT 
CTGCACAGCT TATACCAGGC ACGGATTGTG AGCCATGCCA ACACGGGCGT TTTGAAGAGA 
ATGGAGTGGC TCTTGGAACT GATGGGTTAT ATTAGAAATG TTGCGTACCA GTCAACATCC 
TTTCAGAATA CGGCTCTTGA TGAGGCTTTG GACTTCTTCT TGCTGATATT TGCAACCGCA 
GTGGTTGCAT GGGCCGACCA CACTGCCCCT CTCCTCCTTG GTCTCAGTGC CAGTTGGTTG 
CCATGGCATC AGGAGAATGG CCCGGCTGGG CCAGTACCAA GCTTCCTTGG CAGGAGTCCA 
ATGCACAGGG TCACTGTGCA GGAGGTTCTC ACTCTCCTTC CCAATAGCAT GGCTCTGCTG 
CTGCAGAAAG AGCCATGGAA GGAAGAGACC CAGAAGTTCA TTGACTGGCT ATTCAGCATC 
ATGGAAAGCC CTAAAGAAGC CCTCTCAGCA AAGTCCAGGG ATCTTTTGAA AGCCACCCTG 
CTGTCCTTGA GAGTTCTCCC AGAGTTTAAG AAGAAAGCTG TATGGACCAG AGCGTATGGT 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FOCAD ( XM_024252332.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024108100.1 Pongo abelii focadhesin (FOCAD), transcript variant X7, mRNA.

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CloneID	OSa186756
Clone ID Related Accession (Same CDS sequence)	XM_009244392.2 , XM_024252331.1 , XM_024252332.1 , XM_024252334.1 , XM_009244394.2
Accession Version	XM_024252332.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	focadhesin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036912.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGATG ATATCAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA ATCACAGGCT 
GCGGGTCATC TTATTGCTGC AGTCCTAAAG GAGAATGGTT TTTCAGAAAA GATTTACCAA 
TCTACAAATC AGACTCCTGC TTTGAACTTG CTGTGGGAGA AGTGTTGCAG TGACAATGTA 
GTGGTTCGAA CAGCCTGCTG TGAAGGTCTG GTGGCACTCG TTGCTCAGGA TCATGCAGAG 
TTCAGCTATG TTCTCAATGG GATACTCAAC TTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCATTATGAA ATTACTACAA ATGCAAGCTC TTAAGGAAGG ACAAGGTGGG 
GAAAAGAATA TTCAGAGTGT ATATACCATT AGAAATCATC CTCATCCTTT GATCACTGTG 
CTTGAACACA GACCTGATTG TTGGCCAGTG TTTTTGCAGC AGCTGACAGC ATTTTTCCAG 
CAGTGCCCTG AAAGGTCAGA AGTTTCATGC ATTCAAATAA TGGCACCATT TCTGTGGTAT 
CTGTATTGTG AACCATCTCA GTTACAAGAA TATGCTAAAC TCCGACTAGC CCTGCTGAAA 
GTCTTACTTC AACCCCAGGT TCTTTGTGAC AAGGATCAAC CATCAATACT GCAACAGCAG 
ATACTTCAAC TGTGTTGTGA CATAGTTCCA TGTTTGCAGA TAAAAGATTT GATACAGACA 
ACAGAAGCGA TGATGTTTAT TGAGGAAATA TATTTAAGCC TTTTGCGTCA TCCTGTTTTC 
TGGAAAATTC AGCTTACCCA GATGACTCTT CAGCTGCTGT GTGTCGGTGA AGTCAGCTTA 
AAGATAACTG GTGAATGTTC ATCTTCAATT CACCTCTTAG AGCACAGTGT TGAACTTCTG 
AAGGAGGATT TTCCTGTTGA ACTGGTCATA ATTGGAATAG CTTTACTACT TCTACAGACT 
CCAGCAAGTC AGCAGAAGCC AATCTTAAAT CTAGCTTTGA AGCTCTTCTC TGTTACTGAG 
GATCAGAAAA TCCCAAAGTC CTCTCTGCTG CTAGTGATGC CAATTCTGCA GATACTGTCT 
TCTACTGCCT TGGAAGACTG TATATCTGTG GATGAAGAAG GTCCCTCTAG GCAGCAGTTG 
GCTCTAAACC TTTTGGAAAT GATACAGCAG GAATGTTACA GAGATGACCA CCAAAAGCTC 
TCCTACAAGC TTGTGTGCCC TGTAACCAGT ATGTATGGTT CAGTATTTAC AGCCTGGAGG 
ATTCTTGAAG TAATGACAGA CTCCTCTGCT GCAAGTGACT GGTTGGCTTC AGTAGAGTCA 
TTGCTTCCTA TTACTACTGT GATCCCTGTG CCTGCCTTTC TTCTGCTGGC TCACCTCCTT 
GTTGAAGACA AAGGACAAAA TCTTCACCAA ATACTCAAGG TCACTACAGA ATTAGCCCAA 
GCAGATTCCT CCCAGGTGCC AAATCTGATT CCAGTTTTGA TGTTCAAATT GGGAAGACCA 
CTGGAACCTA TATTATATAA TGATATATTG TATACTTTAC CTAAGCTTGG TGTTCACAAG 
GTGTGTATAG GACAAATTCT ACGAGTAATA CAACTGCTTG GAACCACACC ACGACTAAGA 
GCTGTCACTT TGCGCTTGCT GACATCTTTG TGGGAAAAGC AGGACCGAGT CTATCCTGAA 
CTGCAGCGTT TCATGGCTGT GTCTGATGTA CCTTCTCTTT CGGTGGGCAA GGAAGTCCAA 
TGGGAGAAAC TGATTGCAAA AGCAGCATCA ATCAGAGATA TATGTAAGCA GAGGCCATAT 
CAACATGGTG CAGATATGCT GGCAGCTATT TCTCAGGTGT TGAATGAATG CACCAAGCCT 
GATCAAGCTA CTCCAGCAGC CTTGGTATTA CAAGGTCTTC ATGCACTCTG TCAAGCTGAG 
GTTGTTTGCA TTCGCTCCAC TTGGAATGCT CTCTCTCCAA AGCTGAGTTG TGACACAAGA 
CCTCTCATTC TGAAGACACT GAGTGAACTA TTTTCTCTAG TTCCTTCCTT AACAGTCAAT 
ACAACTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAC TCATACTCAA 
AACAAGGACC CAATTGTGGC AAATGCTGCA TATAGATCCC TGGCCAATTT TAGTGCAGGA 
GAACACACCA TTCTTCATCT GCCTGAAAAG ATAAGACCAG AAATTCCCAT TCCTGAAGAG 
TTAGATGATG ATGAAGATGA TGAGGATGTG GATCTTTCAG TTCCTGGCTC TTGCTATCTC 
AAACTGTTGT CACTCACTCC CCCTTTGGTT TTACCAGCTT TGGAGGAATT TTTTACATCA 
CTTGTGAAGC AAGAAATGGT GAATATGCCT CGTGGGATAT ATCACTCTGC ATTAAAAGGA 
GGTGTCCGCT CAGACCAAGG AAAGACTGTA GCAGGAATCC CCAATTTTAT ATTGAAAATG 
TATGAAACAA ACAAGCAACC AGGATTGAAA CCTGGCCTTG CAGGTGGTAT GTTATTTTGC 
TATGATGTTT CCATGTATCA GAGTAAAGAT GGAAAACCAT TGAATAGACT GATGGCCAGC 
AGAGGGCGAA GTTTCAAGCA GACATCACTT GCTCTTGTAC ATGAGGTTCA TATCCAGCTT 
TCAGAGTGGC ACCGTGCGAT TTTTCTTCCA CAGGCCTGGC TTGCATACAT GAATCGAGCT 
TATCATGCCA TTTTACAGGG AAGACTAGGA GAGCTGGAGT TGCAGTTAAA ACATGGAAAA 
GAAGAACCTG AGGAGGTGCA GTACAAAAAA AGCACAGCTT GGCTCTGGGT TAGAGACATG 
CTGACTGATG AGATCACCAA GGCAGCTGCA AAGGAGAGTC CGGTAGTGAA AGGCAATGCG 
CTGTTAGCTC TAAGCAGCCT TGCTGTCGTC GTATCTAGAC ATGAAGCCAG CGTCTCCTCA 
GACTCTGACG GGCTCCTGGA GGTTCAACCT AATTTCCTTT CAATGAAAGA GTGGGTTTCC 
ATGATACTTG ATACACTCTT GGTCATTGTG GATAGCCATT ACCAACCCAG AGGGCAACTT 
CTCTCCTGGT TTTATTATAA GTCCTATTCT GGTGAAAACA CAGCTAGTGC CATTGCCCGT 
TCTGCTGCCG CCACGGCTTT GTCTCTCCTT GTGCCAGTTT TCATTATCTC TTGCAAAGAG 
AAGGTTGAGG AAATCCTGAA CATGCTGACT GCCAGGTTAC CTGGGAAACC AAGTGCTGAT 
GAGTCTCAAG CCGTGCAAAT CCACATGGGC CTTGCTTTAG GGATGTTTCT CTCTCGCTTG 
TGTGAAGAGA AACTCAGTGA TATATGTGGC CAAGAGATGA ACCTTCTTCT GATGAAGTCG 
TTGGATGCCC TGGAAAATTG CTGCTTTGAC ACTAGTCTTG AATACAACAC GGGCTGTATA 
TTGGGAGTTG GACTTGTTCT GTCCCTCATG AGTCACAGCA GCCAAATGCA GTCCTGCGTT 
CACGTGACAG CATTGCTTCG GAAGCTGTCT GCATACCTAG ATGACAGTGG GAGCCAGAGC 
AGAACGTTTC AGGAGGTCCT TGCCTACACA CTTAGCTGTG TATGTACATC AGCGTTCAGT 
GCTGGAATTA TTGAGGCTAC AGAGGCTGAG GATGTTATGA ACAAGCTTCG ACTGTTAGTG 
GAGAATAGCC AGCAGACTTC AGGTTTTGCC CTGGCTTTAG GAAACGTAGT TCATGGATTG 
TCTGTGTGTG GACATGGAAA AGCTGAAGAC TTGGGCAGCA AACTACTCCC TGCCTGGATC 
AGAATTGTTC TAACAGAGGG CACTCCCACA ATGCTTTGTC TGGCAGCTCT TCATGGCATG 
GTGGCCCTGG TAGGCTCTGA AGGGGATGTA ATGCAGCTGA AATCAGAAGC CATCCAGACC 
TCTCATTTTC AAGGCAGACT TAATGAAGTC ATTAGAACCT TAACTCAGGT CATTAGTGTC 
TCTGGGGTGA TTGGTCTCCA GTCAAATGCA GTCTGGCTTC TTGGACATCT TCATCTATCT 
ACTCTATCCT CAAGTCAAAG TAGAGCCTCT GTTCCTACTG ACTATAGCTA TTTGCCTGAA 
AGCAGTTTTA TTGGAGCAGC TATTGGCTTC TTCATTACAG GAGGAAAAAA AGGTCCTGAA 
TCTGTGCCTC CTTCCCTTCT TAAAGTAGTG ATGAAACCCA TAGCAACTGT TGGAGAAAGC 
TACCAATATC CTCCTGTGAA CTGGGCTGCA CTTCTCTCTC CACTTATGAG GCTAAATTTT 
GGTGAAGAGA TCCAGCAACT GTGCCTTGAA ATTATGGTGA CCCAGGCACA GTCATCCCAG 
AATGCAGCTG CACTATTGGG CTTGTGGGTG ACACCACCAC TGATCCACAG TCTGAGTCTG 
AATACCAAGA GATATCTCCT GATATCTGCA CCTCTGTGGA TAAAACACAT CTCTGATGAA 
CAGATCCTGG GTTTTGTTGA AAATTTAATG GTGGCAGTTT TTAAAGCAGC TTCCCCACTT 
GGAAATCCTG AGCTATGCCC GAGTGCTTTA CAGGGTCTGA GCCAGGCCAT GAAACTGCCC 
AGCCCTGCTC ACCACCTCTG GAGTCTGCTC TCTGAAGCTA CTGGGAAAAT TTTTGACCTC 
CTGCCAAATA AGATTCGGAG AAAGGATCTA GAGCTGTATA TCAGCATAGC AAAATGCCTC 
TTAGAAATGA CAGATGATGA TGCCAATCGG ATCGCCCAGG TTACTAAGAG CAACATAGAA 
AAGGCTGCCT TTGTCAAACT GTACTTAGTC TCTCAAGGAC GATTCCCCTT GGTGAACCTG 
ACCGATATGC TGAGTGTTGC TGTGCAGCAC CGTGAGAAAG AGGTGTTGGC CTGGATGATT 
CTGCACAGCT TATACCAGGC ACGGATTGTG AGCCATGCCA ACACGGGCGT TTTGAAGAGA 
ATGGAGTGGC TCTTGGAACT GATGGGTTAT ATTAGAAATG TTGCGTACCA GTCAACATCC 
TTTCAGAATA CGGCTCTTGA TGAGGCTTTG GACTTCTTCT TGCTGATATT TGCAACCGCA 
GTGGTTGCAT GGGCCGACCA CACTGCCCCT CTCCTCCTTG GTCTCAGTGC CAGTTGGTTG 
CCATGGCATC AGGAGAATGG CCCGGCTGGG CCAGTACCAA GCTTCCTTGG CAGGAGTCCA 
ATGCACAGGG TCACTGTGCA GGAGGTTCTC ACTCTCCTTC CCAATAGCAT GGCTCTGCTG 
CTGCAGAAAG AGCCATGGAA GGAAGAGACC CAGAAGTTCA TTGACTGGCT ATTCAGCATC 
ATGGAAAGCC CTAAAGAAGC CCTCTCAGCA AAGTCCAGGG ATCTTTTGAA AGCCACCCTG 
CTGTCCTTGA GAGTTCTCCC AGAGTTTAAG AAGAAAGCTG TATGGACCAG AGCGTATGGT 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024108100.1
CDS	203..5608
Translation	MSDDIRKRFEFPNSLIQSQAAGHLIAAVLKENGFSEKIYQSTNQTPALNLLWEKCCSDNVVVRTACCEGLVALVAQDHAEFSYVLNGILNLIPSTRNTHGLIKAIMKLLQMQALKEGQGGEKNIQSVYTIRNHPHPLITVLEHRPDCWPVFLQQLTAFFQQCPERSEVSCIQIMAPFLWYLYCEPSQLQEYAKLRLALLKVLLQPQVLCDKDQPSILQQQILQLCCDIVPCLQIKDLIQTTEAMMFIEEIYLSLLRHPVFWKIQLTQMTLQLLCVGEVSLKITGECSSSIHLLEHSVELLKEDFPVELVIIGIALLLLQTPASQQKPILNLALKLFSVTEDQKIPKSSLLLVMPILQILSSTALEDCISVDEEGPSRQQLALNLLEMIQQECYRDDHQKLSYKLVCPVTSMYGSVFTAWRILEVMTDSSAASDWLASVESLLPITTVIPVPAFLLLAHLLVEDKGQNLHQILKVTTELAQADSSQVPNLIPVLMFKLGRPLEPILYNDILYTLPKLGVHKVCIGQILRVIQLLGTTPRLRAVTLRLLTSLWEKQDRVYPELQRFMAVSDVPSLSVGKEVQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQATPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDTRPLILKTLSELFSLVPSLTVNTTEYENFKVQVLSFLWTHTQNKDPIVANAAYRSLANFSAGEHTILHLPEKIRPEIPIPEELDDDEDDEDVDLSVPGSCYLKLLSLTPPLVLPALEEFFTSLVKQEMVNMPRGIYHSALKGGVRSDQGKTVAGIPNFILKMYETNKQPGLKPGLAGGMLFCYDVSMYQSKDGKPLNRLMASRGRSFKQTSLALVHEVHIQLSEWHRAIFLPQAWLAYMNRAYHAILQGRLGELELQLKHGKEEPEEVQYKKSTAWLWVRDMLTDEITKAAAKESPVVKGNALLALSSLAVVVSRHEASVSSDSDGLLEVQPNFLSMKEWVSMILDTLLVIVDSHYQPRGQLLSWFYYKSYSGENTASAIARSAAATALSLLVPVFIISCKEKVEEILNMLTARLPGKPSADESQAVQIHMGLALGMFLSRLCEEKLSDICGQEMNLLLMKSLDALENCCFDTSLEYNTGCILGVGLVLSLMSHSSQMQSCVHVTALLRKLSAYLDDSGSQSRTFQEVLAYTLSCVCTSAFSAGIIEATEAEDVMNKLRLLVENSQQTSGFALALGNVVHGLSVCGHGKAEDLGSKLLPAWIRIVLTEGTPTMLCLAALHGMVALVGSEGDVMQLKSEAIQTSHFQGRLNEVIRTLTQVISVSGVIGLQSNAVWLLGHLHLSTLSSSQSRASVPTDYSYLPESSFIGAAIGFFITGGKKGPESVPPSLLKVVMKPIATVGESYQYPPVNWAALLSPLMRLNFGEEIQQLCLEIMVTQAQSSQNAAALLGLWVTPPLIHSLSLNTKRYLLISAPLWIKHISDEQILGFVENLMVAVFKAASPLGNPELCPSALQGLSQAMKLPSPAHHLWSLLSEATGKIFDLLPNKIRRKDLELYISIAKCLLEMTDDDANRIAQVTKSNIEKAAFVKLYLVSQGRFPLVNLTDMLSVAVQHREKEVLAWMILHSLYQARIVSHANTGVLKRMEWLLELMGYIRNVAYQSTSFQNTALDEALDFFLLIFATAVVAWADHTAPLLLGLSASWLPWHQENGPAGPVPSFLGRSPMHRVTVQEVLTLLPNSMALLLQKEPWKEETQKFIDWLFSIMESPKEALSAKSRDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_024252332.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii focadhesin (FOCAD), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024252332.1

ORF Insert Sequence:

ATGTCAGATG ATATCAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA ATCACAGGCT 
GCGGGTCATC TTATTGCTGC AGTCCTAAAG GAGAATGGTT TTTCAGAAAA GATTTACCAA 
TCTACAAATC AGACTCCTGC TTTGAACTTG CTGTGGGAGA AGTGTTGCAG TGACAATGTA 
GTGGTTCGAA CAGCCTGCTG TGAAGGTCTG GTGGCACTCG TTGCTCAGGA TCATGCAGAG 
TTCAGCTATG TTCTCAATGG GATACTCAAC TTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCATTATGAA ATTACTACAA ATGCAAGCTC TTAAGGAAGG ACAAGGTGGG 
GAAAAGAATA TTCAGAGTGT ATATACCATT AGAAATCATC CTCATCCTTT GATCACTGTG 
CTTGAACACA GACCTGATTG TTGGCCAGTG TTTTTGCAGC AGCTGACAGC ATTTTTCCAG 
CAGTGCCCTG AAAGGTCAGA AGTTTCATGC ATTCAAATAA TGGCACCATT TCTGTGGTAT 
CTGTATTGTG AACCATCTCA GTTACAAGAA TATGCTAAAC TCCGACTAGC CCTGCTGAAA 
GTCTTACTTC AACCCCAGGT TCTTTGTGAC AAGGATCAAC CATCAATACT GCAACAGCAG 
ATACTTCAAC TGTGTTGTGA CATAGTTCCA TGTTTGCAGA TAAAAGATTT GATACAGACA 
ACAGAAGCGA TGATGTTTAT TGAGGAAATA TATTTAAGCC TTTTGCGTCA TCCTGTTTTC 
TGGAAAATTC AGCTTACCCA GATGACTCTT CAGCTGCTGT GTGTCGGTGA AGTCAGCTTA 
AAGATAACTG GTGAATGTTC ATCTTCAATT CACCTCTTAG AGCACAGTGT TGAACTTCTG 
AAGGAGGATT TTCCTGTTGA ACTGGTCATA ATTGGAATAG CTTTACTACT TCTACAGACT 
CCAGCAAGTC AGCAGAAGCC AATCTTAAAT CTAGCTTTGA AGCTCTTCTC TGTTACTGAG 
GATCAGAAAA TCCCAAAGTC CTCTCTGCTG CTAGTGATGC CAATTCTGCA GATACTGTCT 
TCTACTGCCT TGGAAGACTG TATATCTGTG GATGAAGAAG GTCCCTCTAG GCAGCAGTTG 
GCTCTAAACC TTTTGGAAAT GATACAGCAG GAATGTTACA GAGATGACCA CCAAAAGCTC 
TCCTACAAGC TTGTGTGCCC TGTAACCAGT ATGTATGGTT CAGTATTTAC AGCCTGGAGG 
ATTCTTGAAG TAATGACAGA CTCCTCTGCT GCAAGTGACT GGTTGGCTTC AGTAGAGTCA 
TTGCTTCCTA TTACTACTGT GATCCCTGTG CCTGCCTTTC TTCTGCTGGC TCACCTCCTT 
GTTGAAGACA AAGGACAAAA TCTTCACCAA ATACTCAAGG TCACTACAGA ATTAGCCCAA 
GCAGATTCCT CCCAGGTGCC AAATCTGATT CCAGTTTTGA TGTTCAAATT GGGAAGACCA 
CTGGAACCTA TATTATATAA TGATATATTG TATACTTTAC CTAAGCTTGG TGTTCACAAG 
GTGTGTATAG GACAAATTCT ACGAGTAATA CAACTGCTTG GAACCACACC ACGACTAAGA 
GCTGTCACTT TGCGCTTGCT GACATCTTTG TGGGAAAAGC AGGACCGAGT CTATCCTGAA 
CTGCAGCGTT TCATGGCTGT GTCTGATGTA CCTTCTCTTT CGGTGGGCAA GGAAGTCCAA 
TGGGAGAAAC TGATTGCAAA AGCAGCATCA ATCAGAGATA TATGTAAGCA GAGGCCATAT 
CAACATGGTG CAGATATGCT GGCAGCTATT TCTCAGGTGT TGAATGAATG CACCAAGCCT 
GATCAAGCTA CTCCAGCAGC CTTGGTATTA CAAGGTCTTC ATGCACTCTG TCAAGCTGAG 
GTTGTTTGCA TTCGCTCCAC TTGGAATGCT CTCTCTCCAA AGCTGAGTTG TGACACAAGA 
CCTCTCATTC TGAAGACACT GAGTGAACTA TTTTCTCTAG TTCCTTCCTT AACAGTCAAT 
ACAACTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAC TCATACTCAA 
AACAAGGACC CAATTGTGGC AAATGCTGCA TATAGATCCC TGGCCAATTT TAGTGCAGGA 
GAACACACCA TTCTTCATCT GCCTGAAAAG ATAAGACCAG AAATTCCCAT TCCTGAAGAG 
TTAGATGATG ATGAAGATGA TGAGGATGTG GATCTTTCAG TTCCTGGCTC TTGCTATCTC 
AAACTGTTGT CACTCACTCC CCCTTTGGTT TTACCAGCTT TGGAGGAATT TTTTACATCA 
CTTGTGAAGC AAGAAATGGT GAATATGCCT CGTGGGATAT ATCACTCTGC ATTAAAAGGA 
GGTGTCCGCT CAGACCAAGG AAAGACTGTA GCAGGAATCC CCAATTTTAT ATTGAAAATG 
TATGAAACAA ACAAGCAACC AGGATTGAAA CCTGGCCTTG CAGGTGGTAT GTTATTTTGC 
TATGATGTTT CCATGTATCA GAGTAAAGAT GGAAAACCAT TGAATAGACT GATGGCCAGC 
AGAGGGCGAA GTTTCAAGCA GACATCACTT GCTCTTGTAC ATGAGGTTCA TATCCAGCTT 
TCAGAGTGGC ACCGTGCGAT TTTTCTTCCA CAGGCCTGGC TTGCATACAT GAATCGAGCT 
TATCATGCCA TTTTACAGGG AAGACTAGGA GAGCTGGAGT TGCAGTTAAA ACATGGAAAA 
GAAGAACCTG AGGAGGTGCA GTACAAAAAA AGCACAGCTT GGCTCTGGGT TAGAGACATG 
CTGACTGATG AGATCACCAA GGCAGCTGCA AAGGAGAGTC CGGTAGTGAA AGGCAATGCG 
CTGTTAGCTC TAAGCAGCCT TGCTGTCGTC GTATCTAGAC ATGAAGCCAG CGTCTCCTCA 
GACTCTGACG GGCTCCTGGA GGTTCAACCT AATTTCCTTT CAATGAAAGA GTGGGTTTCC 
ATGATACTTG ATACACTCTT GGTCATTGTG GATAGCCATT ACCAACCCAG AGGGCAACTT 
CTCTCCTGGT TTTATTATAA GTCCTATTCT GGTGAAAACA CAGCTAGTGC CATTGCCCGT 
TCTGCTGCCG CCACGGCTTT GTCTCTCCTT GTGCCAGTTT TCATTATCTC TTGCAAAGAG 
AAGGTTGAGG AAATCCTGAA CATGCTGACT GCCAGGTTAC CTGGGAAACC AAGTGCTGAT 
GAGTCTCAAG CCGTGCAAAT CCACATGGGC CTTGCTTTAG GGATGTTTCT CTCTCGCTTG 
TGTGAAGAGA AACTCAGTGA TATATGTGGC CAAGAGATGA ACCTTCTTCT GATGAAGTCG 
TTGGATGCCC TGGAAAATTG CTGCTTTGAC ACTAGTCTTG AATACAACAC GGGCTGTATA 
TTGGGAGTTG GACTTGTTCT GTCCCTCATG AGTCACAGCA GCCAAATGCA GTCCTGCGTT 
CACGTGACAG CATTGCTTCG GAAGCTGTCT GCATACCTAG ATGACAGTGG GAGCCAGAGC 
AGAACGTTTC AGGAGGTCCT TGCCTACACA CTTAGCTGTG TATGTACATC AGCGTTCAGT 
GCTGGAATTA TTGAGGCTAC AGAGGCTGAG GATGTTATGA ACAAGCTTCG ACTGTTAGTG 
GAGAATAGCC AGCAGACTTC AGGTTTTGCC CTGGCTTTAG GAAACGTAGT TCATGGATTG 
TCTGTGTGTG GACATGGAAA AGCTGAAGAC TTGGGCAGCA AACTACTCCC TGCCTGGATC 
AGAATTGTTC TAACAGAGGG CACTCCCACA ATGCTTTGTC TGGCAGCTCT TCATGGCATG 
GTGGCCCTGG TAGGCTCTGA AGGGGATGTA ATGCAGCTGA AATCAGAAGC CATCCAGACC 
TCTCATTTTC AAGGCAGACT TAATGAAGTC ATTAGAACCT TAACTCAGGT CATTAGTGTC 
TCTGGGGTGA TTGGTCTCCA GTCAAATGCA GTCTGGCTTC TTGGACATCT TCATCTATCT 
ACTCTATCCT CAAGTCAAAG TAGAGCCTCT GTTCCTACTG ACTATAGCTA TTTGCCTGAA 
AGCAGTTTTA TTGGAGCAGC TATTGGCTTC TTCATTACAG GAGGAAAAAA AGGTCCTGAA 
TCTGTGCCTC CTTCCCTTCT TAAAGTAGTG ATGAAACCCA TAGCAACTGT TGGAGAAAGC 
TACCAATATC CTCCTGTGAA CTGGGCTGCA CTTCTCTCTC CACTTATGAG GCTAAATTTT 
GGTGAAGAGA TCCAGCAACT GTGCCTTGAA ATTATGGTGA CCCAGGCACA GTCATCCCAG 
AATGCAGCTG CACTATTGGG CTTGTGGGTG ACACCACCAC TGATCCACAG TCTGAGTCTG 
AATACCAAGA GATATCTCCT GATATCTGCA CCTCTGTGGA TAAAACACAT CTCTGATGAA 
CAGATCCTGG GTTTTGTTGA AAATTTAATG GTGGCAGTTT TTAAAGCAGC TTCCCCACTT 
GGAAATCCTG AGCTATGCCC GAGTGCTTTA CAGGGTCTGA GCCAGGCCAT GAAACTGCCC 
AGCCCTGCTC ACCACCTCTG GAGTCTGCTC TCTGAAGCTA CTGGGAAAAT TTTTGACCTC 
CTGCCAAATA AGATTCGGAG AAAGGATCTA GAGCTGTATA TCAGCATAGC AAAATGCCTC 
TTAGAAATGA CAGATGATGA TGCCAATCGG ATCGCCCAGG TTACTAAGAG CAACATAGAA 
AAGGCTGCCT TTGTCAAACT GTACTTAGTC TCTCAAGGAC GATTCCCCTT GGTGAACCTG 
ACCGATATGC TGAGTGTTGC TGTGCAGCAC CGTGAGAAAG AGGTGTTGGC CTGGATGATT 
CTGCACAGCT TATACCAGGC ACGGATTGTG AGCCATGCCA ACACGGGCGT TTTGAAGAGA 
ATGGAGTGGC TCTTGGAACT GATGGGTTAT ATTAGAAATG TTGCGTACCA GTCAACATCC 
TTTCAGAATA CGGCTCTTGA TGAGGCTTTG GACTTCTTCT TGCTGATATT TGCAACCGCA 
GTGGTTGCAT GGGCCGACCA CACTGCCCCT CTCCTCCTTG GTCTCAGTGC CAGTTGGTTG 
CCATGGCATC AGGAGAATGG CCCGGCTGGG CCAGTACCAA GCTTCCTTGG CAGGAGTCCA 
ATGCACAGGG TCACTGTGCA GGAGGTTCTC ACTCTCCTTC CCAATAGCAT GGCTCTGCTG 
CTGCAGAAAG AGCCATGGAA GGAAGAGACC CAGAAGTTCA TTGACTGGCT ATTCAGCATC 
ATGGAAAGCC CTAAAGAAGC CCTCTCAGCA AAGTCCAGGG ATCTTTTGAA AGCCACCCTG 
CTGTCCTTGA GAGTTCTCCC AGAGTTTAAG AAGAAAGCTG TATGGACCAG AGCGTATGGT 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FOCAD ( XM_024252334.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024108102.1 Pongo abelii focadhesin (FOCAD), transcript variant X8, mRNA.

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CloneID	OSa186756
Clone ID Related Accession (Same CDS sequence)	XM_009244392.2 , XM_024252331.1 , XM_024252332.1 , XM_024252334.1 , XM_009244394.2
Accession Version	XM_024252334.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	focadhesin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036912.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGATG ATATCAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA ATCACAGGCT 
GCGGGTCATC TTATTGCTGC AGTCCTAAAG GAGAATGGTT TTTCAGAAAA GATTTACCAA 
TCTACAAATC AGACTCCTGC TTTGAACTTG CTGTGGGAGA AGTGTTGCAG TGACAATGTA 
GTGGTTCGAA CAGCCTGCTG TGAAGGTCTG GTGGCACTCG TTGCTCAGGA TCATGCAGAG 
TTCAGCTATG TTCTCAATGG GATACTCAAC TTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCATTATGAA ATTACTACAA ATGCAAGCTC TTAAGGAAGG ACAAGGTGGG 
GAAAAGAATA TTCAGAGTGT ATATACCATT AGAAATCATC CTCATCCTTT GATCACTGTG 
CTTGAACACA GACCTGATTG TTGGCCAGTG TTTTTGCAGC AGCTGACAGC ATTTTTCCAG 
CAGTGCCCTG AAAGGTCAGA AGTTTCATGC ATTCAAATAA TGGCACCATT TCTGTGGTAT 
CTGTATTGTG AACCATCTCA GTTACAAGAA TATGCTAAAC TCCGACTAGC CCTGCTGAAA 
GTCTTACTTC AACCCCAGGT TCTTTGTGAC AAGGATCAAC CATCAATACT GCAACAGCAG 
ATACTTCAAC TGTGTTGTGA CATAGTTCCA TGTTTGCAGA TAAAAGATTT GATACAGACA 
ACAGAAGCGA TGATGTTTAT TGAGGAAATA TATTTAAGCC TTTTGCGTCA TCCTGTTTTC 
TGGAAAATTC AGCTTACCCA GATGACTCTT CAGCTGCTGT GTGTCGGTGA AGTCAGCTTA 
AAGATAACTG GTGAATGTTC ATCTTCAATT CACCTCTTAG AGCACAGTGT TGAACTTCTG 
AAGGAGGATT TTCCTGTTGA ACTGGTCATA ATTGGAATAG CTTTACTACT TCTACAGACT 
CCAGCAAGTC AGCAGAAGCC AATCTTAAAT CTAGCTTTGA AGCTCTTCTC TGTTACTGAG 
GATCAGAAAA TCCCAAAGTC CTCTCTGCTG CTAGTGATGC CAATTCTGCA GATACTGTCT 
TCTACTGCCT TGGAAGACTG TATATCTGTG GATGAAGAAG GTCCCTCTAG GCAGCAGTTG 
GCTCTAAACC TTTTGGAAAT GATACAGCAG GAATGTTACA GAGATGACCA CCAAAAGCTC 
TCCTACAAGC TTGTGTGCCC TGTAACCAGT ATGTATGGTT CAGTATTTAC AGCCTGGAGG 
ATTCTTGAAG TAATGACAGA CTCCTCTGCT GCAAGTGACT GGTTGGCTTC AGTAGAGTCA 
TTGCTTCCTA TTACTACTGT GATCCCTGTG CCTGCCTTTC TTCTGCTGGC TCACCTCCTT 
GTTGAAGACA AAGGACAAAA TCTTCACCAA ATACTCAAGG TCACTACAGA ATTAGCCCAA 
GCAGATTCCT CCCAGGTGCC AAATCTGATT CCAGTTTTGA TGTTCAAATT GGGAAGACCA 
CTGGAACCTA TATTATATAA TGATATATTG TATACTTTAC CTAAGCTTGG TGTTCACAAG 
GTGTGTATAG GACAAATTCT ACGAGTAATA CAACTGCTTG GAACCACACC ACGACTAAGA 
GCTGTCACTT TGCGCTTGCT GACATCTTTG TGGGAAAAGC AGGACCGAGT CTATCCTGAA 
CTGCAGCGTT TCATGGCTGT GTCTGATGTA CCTTCTCTTT CGGTGGGCAA GGAAGTCCAA 
TGGGAGAAAC TGATTGCAAA AGCAGCATCA ATCAGAGATA TATGTAAGCA GAGGCCATAT 
CAACATGGTG CAGATATGCT GGCAGCTATT TCTCAGGTGT TGAATGAATG CACCAAGCCT 
GATCAAGCTA CTCCAGCAGC CTTGGTATTA CAAGGTCTTC ATGCACTCTG TCAAGCTGAG 
GTTGTTTGCA TTCGCTCCAC TTGGAATGCT CTCTCTCCAA AGCTGAGTTG TGACACAAGA 
CCTCTCATTC TGAAGACACT GAGTGAACTA TTTTCTCTAG TTCCTTCCTT AACAGTCAAT 
ACAACTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAC TCATACTCAA 
AACAAGGACC CAATTGTGGC AAATGCTGCA TATAGATCCC TGGCCAATTT TAGTGCAGGA 
GAACACACCA TTCTTCATCT GCCTGAAAAG ATAAGACCAG AAATTCCCAT TCCTGAAGAG 
TTAGATGATG ATGAAGATGA TGAGGATGTG GATCTTTCAG TTCCTGGCTC TTGCTATCTC 
AAACTGTTGT CACTCACTCC CCCTTTGGTT TTACCAGCTT TGGAGGAATT TTTTACATCA 
CTTGTGAAGC AAGAAATGGT GAATATGCCT CGTGGGATAT ATCACTCTGC ATTAAAAGGA 
GGTGTCCGCT CAGACCAAGG AAAGACTGTA GCAGGAATCC CCAATTTTAT ATTGAAAATG 
TATGAAACAA ACAAGCAACC AGGATTGAAA CCTGGCCTTG CAGGTGGTAT GTTATTTTGC 
TATGATGTTT CCATGTATCA GAGTAAAGAT GGAAAACCAT TGAATAGACT GATGGCCAGC 
AGAGGGCGAA GTTTCAAGCA GACATCACTT GCTCTTGTAC ATGAGGTTCA TATCCAGCTT 
TCAGAGTGGC ACCGTGCGAT TTTTCTTCCA CAGGCCTGGC TTGCATACAT GAATCGAGCT 
TATCATGCCA TTTTACAGGG AAGACTAGGA GAGCTGGAGT TGCAGTTAAA ACATGGAAAA 
GAAGAACCTG AGGAGGTGCA GTACAAAAAA AGCACAGCTT GGCTCTGGGT TAGAGACATG 
CTGACTGATG AGATCACCAA GGCAGCTGCA AAGGAGAGTC CGGTAGTGAA AGGCAATGCG 
CTGTTAGCTC TAAGCAGCCT TGCTGTCGTC GTATCTAGAC ATGAAGCCAG CGTCTCCTCA 
GACTCTGACG GGCTCCTGGA GGTTCAACCT AATTTCCTTT CAATGAAAGA GTGGGTTTCC 
ATGATACTTG ATACACTCTT GGTCATTGTG GATAGCCATT ACCAACCCAG AGGGCAACTT 
CTCTCCTGGT TTTATTATAA GTCCTATTCT GGTGAAAACA CAGCTAGTGC CATTGCCCGT 
TCTGCTGCCG CCACGGCTTT GTCTCTCCTT GTGCCAGTTT TCATTATCTC TTGCAAAGAG 
AAGGTTGAGG AAATCCTGAA CATGCTGACT GCCAGGTTAC CTGGGAAACC AAGTGCTGAT 
GAGTCTCAAG CCGTGCAAAT CCACATGGGC CTTGCTTTAG GGATGTTTCT CTCTCGCTTG 
TGTGAAGAGA AACTCAGTGA TATATGTGGC CAAGAGATGA ACCTTCTTCT GATGAAGTCG 
TTGGATGCCC TGGAAAATTG CTGCTTTGAC ACTAGTCTTG AATACAACAC GGGCTGTATA 
TTGGGAGTTG GACTTGTTCT GTCCCTCATG AGTCACAGCA GCCAAATGCA GTCCTGCGTT 
CACGTGACAG CATTGCTTCG GAAGCTGTCT GCATACCTAG ATGACAGTGG GAGCCAGAGC 
AGAACGTTTC AGGAGGTCCT TGCCTACACA CTTAGCTGTG TATGTACATC AGCGTTCAGT 
GCTGGAATTA TTGAGGCTAC AGAGGCTGAG GATGTTATGA ACAAGCTTCG ACTGTTAGTG 
GAGAATAGCC AGCAGACTTC AGGTTTTGCC CTGGCTTTAG GAAACGTAGT TCATGGATTG 
TCTGTGTGTG GACATGGAAA AGCTGAAGAC TTGGGCAGCA AACTACTCCC TGCCTGGATC 
AGAATTGTTC TAACAGAGGG CACTCCCACA ATGCTTTGTC TGGCAGCTCT TCATGGCATG 
GTGGCCCTGG TAGGCTCTGA AGGGGATGTA ATGCAGCTGA AATCAGAAGC CATCCAGACC 
TCTCATTTTC AAGGCAGACT TAATGAAGTC ATTAGAACCT TAACTCAGGT CATTAGTGTC 
TCTGGGGTGA TTGGTCTCCA GTCAAATGCA GTCTGGCTTC TTGGACATCT TCATCTATCT 
ACTCTATCCT CAAGTCAAAG TAGAGCCTCT GTTCCTACTG ACTATAGCTA TTTGCCTGAA 
AGCAGTTTTA TTGGAGCAGC TATTGGCTTC TTCATTACAG GAGGAAAAAA AGGTCCTGAA 
TCTGTGCCTC CTTCCCTTCT TAAAGTAGTG ATGAAACCCA TAGCAACTGT TGGAGAAAGC 
TACCAATATC CTCCTGTGAA CTGGGCTGCA CTTCTCTCTC CACTTATGAG GCTAAATTTT 
GGTGAAGAGA TCCAGCAACT GTGCCTTGAA ATTATGGTGA CCCAGGCACA GTCATCCCAG 
AATGCAGCTG CACTATTGGG CTTGTGGGTG ACACCACCAC TGATCCACAG TCTGAGTCTG 
AATACCAAGA GATATCTCCT GATATCTGCA CCTCTGTGGA TAAAACACAT CTCTGATGAA 
CAGATCCTGG GTTTTGTTGA AAATTTAATG GTGGCAGTTT TTAAAGCAGC TTCCCCACTT 
GGAAATCCTG AGCTATGCCC GAGTGCTTTA CAGGGTCTGA GCCAGGCCAT GAAACTGCCC 
AGCCCTGCTC ACCACCTCTG GAGTCTGCTC TCTGAAGCTA CTGGGAAAAT TTTTGACCTC 
CTGCCAAATA AGATTCGGAG AAAGGATCTA GAGCTGTATA TCAGCATAGC AAAATGCCTC 
TTAGAAATGA CAGATGATGA TGCCAATCGG ATCGCCCAGG TTACTAAGAG CAACATAGAA 
AAGGCTGCCT TTGTCAAACT GTACTTAGTC TCTCAAGGAC GATTCCCCTT GGTGAACCTG 
ACCGATATGC TGAGTGTTGC TGTGCAGCAC CGTGAGAAAG AGGTGTTGGC CTGGATGATT 
CTGCACAGCT TATACCAGGC ACGGATTGTG AGCCATGCCA ACACGGGCGT TTTGAAGAGA 
ATGGAGTGGC TCTTGGAACT GATGGGTTAT ATTAGAAATG TTGCGTACCA GTCAACATCC 
TTTCAGAATA CGGCTCTTGA TGAGGCTTTG GACTTCTTCT TGCTGATATT TGCAACCGCA 
GTGGTTGCAT GGGCCGACCA CACTGCCCCT CTCCTCCTTG GTCTCAGTGC CAGTTGGTTG 
CCATGGCATC AGGAGAATGG CCCGGCTGGG CCAGTACCAA GCTTCCTTGG CAGGAGTCCA 
ATGCACAGGG TCACTGTGCA GGAGGTTCTC ACTCTCCTTC CCAATAGCAT GGCTCTGCTG 
CTGCAGAAAG AGCCATGGAA GGAAGAGACC CAGAAGTTCA TTGACTGGCT ATTCAGCATC 
ATGGAAAGCC CTAAAGAAGC CCTCTCAGCA AAGTCCAGGG ATCTTTTGAA AGCCACCCTG 
CTGTCCTTGA GAGTTCTCCC AGAGTTTAAG AAGAAAGCTG TATGGACCAG AGCGTATGGT 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024108102.1
CDS	155..5560
Translation	MSDDIRKRFEFPNSLIQSQAAGHLIAAVLKENGFSEKIYQSTNQTPALNLLWEKCCSDNVVVRTACCEGLVALVAQDHAEFSYVLNGILNLIPSTRNTHGLIKAIMKLLQMQALKEGQGGEKNIQSVYTIRNHPHPLITVLEHRPDCWPVFLQQLTAFFQQCPERSEVSCIQIMAPFLWYLYCEPSQLQEYAKLRLALLKVLLQPQVLCDKDQPSILQQQILQLCCDIVPCLQIKDLIQTTEAMMFIEEIYLSLLRHPVFWKIQLTQMTLQLLCVGEVSLKITGECSSSIHLLEHSVELLKEDFPVELVIIGIALLLLQTPASQQKPILNLALKLFSVTEDQKIPKSSLLLVMPILQILSSTALEDCISVDEEGPSRQQLALNLLEMIQQECYRDDHQKLSYKLVCPVTSMYGSVFTAWRILEVMTDSSAASDWLASVESLLPITTVIPVPAFLLLAHLLVEDKGQNLHQILKVTTELAQADSSQVPNLIPVLMFKLGRPLEPILYNDILYTLPKLGVHKVCIGQILRVIQLLGTTPRLRAVTLRLLTSLWEKQDRVYPELQRFMAVSDVPSLSVGKEVQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQATPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDTRPLILKTLSELFSLVPSLTVNTTEYENFKVQVLSFLWTHTQNKDPIVANAAYRSLANFSAGEHTILHLPEKIRPEIPIPEELDDDEDDEDVDLSVPGSCYLKLLSLTPPLVLPALEEFFTSLVKQEMVNMPRGIYHSALKGGVRSDQGKTVAGIPNFILKMYETNKQPGLKPGLAGGMLFCYDVSMYQSKDGKPLNRLMASRGRSFKQTSLALVHEVHIQLSEWHRAIFLPQAWLAYMNRAYHAILQGRLGELELQLKHGKEEPEEVQYKKSTAWLWVRDMLTDEITKAAAKESPVVKGNALLALSSLAVVVSRHEASVSSDSDGLLEVQPNFLSMKEWVSMILDTLLVIVDSHYQPRGQLLSWFYYKSYSGENTASAIARSAAATALSLLVPVFIISCKEKVEEILNMLTARLPGKPSADESQAVQIHMGLALGMFLSRLCEEKLSDICGQEMNLLLMKSLDALENCCFDTSLEYNTGCILGVGLVLSLMSHSSQMQSCVHVTALLRKLSAYLDDSGSQSRTFQEVLAYTLSCVCTSAFSAGIIEATEAEDVMNKLRLLVENSQQTSGFALALGNVVHGLSVCGHGKAEDLGSKLLPAWIRIVLTEGTPTMLCLAALHGMVALVGSEGDVMQLKSEAIQTSHFQGRLNEVIRTLTQVISVSGVIGLQSNAVWLLGHLHLSTLSSSQSRASVPTDYSYLPESSFIGAAIGFFITGGKKGPESVPPSLLKVVMKPIATVGESYQYPPVNWAALLSPLMRLNFGEEIQQLCLEIMVTQAQSSQNAAALLGLWVTPPLIHSLSLNTKRYLLISAPLWIKHISDEQILGFVENLMVAVFKAASPLGNPELCPSALQGLSQAMKLPSPAHHLWSLLSEATGKIFDLLPNKIRRKDLELYISIAKCLLEMTDDDANRIAQVTKSNIEKAAFVKLYLVSQGRFPLVNLTDMLSVAVQHREKEVLAWMILHSLYQARIVSHANTGVLKRMEWLLELMGYIRNVAYQSTSFQNTALDEALDFFLLIFATAVVAWADHTAPLLLGLSASWLPWHQENGPAGPVPSFLGRSPMHRVTVQEVLTLLPNSMALLLQKEPWKEETQKFIDWLFSIMESPKEALSAKSRDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_024252334.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii focadhesin (FOCAD), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024252334.1

ORF Insert Sequence:

ATGTCAGATG ATATCAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA ATCACAGGCT 
GCGGGTCATC TTATTGCTGC AGTCCTAAAG GAGAATGGTT TTTCAGAAAA GATTTACCAA 
TCTACAAATC AGACTCCTGC TTTGAACTTG CTGTGGGAGA AGTGTTGCAG TGACAATGTA 
GTGGTTCGAA CAGCCTGCTG TGAAGGTCTG GTGGCACTCG TTGCTCAGGA TCATGCAGAG 
TTCAGCTATG TTCTCAATGG GATACTCAAC TTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCATTATGAA ATTACTACAA ATGCAAGCTC TTAAGGAAGG ACAAGGTGGG 
GAAAAGAATA TTCAGAGTGT ATATACCATT AGAAATCATC CTCATCCTTT GATCACTGTG 
CTTGAACACA GACCTGATTG TTGGCCAGTG TTTTTGCAGC AGCTGACAGC ATTTTTCCAG 
CAGTGCCCTG AAAGGTCAGA AGTTTCATGC ATTCAAATAA TGGCACCATT TCTGTGGTAT 
CTGTATTGTG AACCATCTCA GTTACAAGAA TATGCTAAAC TCCGACTAGC CCTGCTGAAA 
GTCTTACTTC AACCCCAGGT TCTTTGTGAC AAGGATCAAC CATCAATACT GCAACAGCAG 
ATACTTCAAC TGTGTTGTGA CATAGTTCCA TGTTTGCAGA TAAAAGATTT GATACAGACA 
ACAGAAGCGA TGATGTTTAT TGAGGAAATA TATTTAAGCC TTTTGCGTCA TCCTGTTTTC 
TGGAAAATTC AGCTTACCCA GATGACTCTT CAGCTGCTGT GTGTCGGTGA AGTCAGCTTA 
AAGATAACTG GTGAATGTTC ATCTTCAATT CACCTCTTAG AGCACAGTGT TGAACTTCTG 
AAGGAGGATT TTCCTGTTGA ACTGGTCATA ATTGGAATAG CTTTACTACT TCTACAGACT 
CCAGCAAGTC AGCAGAAGCC AATCTTAAAT CTAGCTTTGA AGCTCTTCTC TGTTACTGAG 
GATCAGAAAA TCCCAAAGTC CTCTCTGCTG CTAGTGATGC CAATTCTGCA GATACTGTCT 
TCTACTGCCT TGGAAGACTG TATATCTGTG GATGAAGAAG GTCCCTCTAG GCAGCAGTTG 
GCTCTAAACC TTTTGGAAAT GATACAGCAG GAATGTTACA GAGATGACCA CCAAAAGCTC 
TCCTACAAGC TTGTGTGCCC TGTAACCAGT ATGTATGGTT CAGTATTTAC AGCCTGGAGG 
ATTCTTGAAG TAATGACAGA CTCCTCTGCT GCAAGTGACT GGTTGGCTTC AGTAGAGTCA 
TTGCTTCCTA TTACTACTGT GATCCCTGTG CCTGCCTTTC TTCTGCTGGC TCACCTCCTT 
GTTGAAGACA AAGGACAAAA TCTTCACCAA ATACTCAAGG TCACTACAGA ATTAGCCCAA 
GCAGATTCCT CCCAGGTGCC AAATCTGATT CCAGTTTTGA TGTTCAAATT GGGAAGACCA 
CTGGAACCTA TATTATATAA TGATATATTG TATACTTTAC CTAAGCTTGG TGTTCACAAG 
GTGTGTATAG GACAAATTCT ACGAGTAATA CAACTGCTTG GAACCACACC ACGACTAAGA 
GCTGTCACTT TGCGCTTGCT GACATCTTTG TGGGAAAAGC AGGACCGAGT CTATCCTGAA 
CTGCAGCGTT TCATGGCTGT GTCTGATGTA CCTTCTCTTT CGGTGGGCAA GGAAGTCCAA 
TGGGAGAAAC TGATTGCAAA AGCAGCATCA ATCAGAGATA TATGTAAGCA GAGGCCATAT 
CAACATGGTG CAGATATGCT GGCAGCTATT TCTCAGGTGT TGAATGAATG CACCAAGCCT 
GATCAAGCTA CTCCAGCAGC CTTGGTATTA CAAGGTCTTC ATGCACTCTG TCAAGCTGAG 
GTTGTTTGCA TTCGCTCCAC TTGGAATGCT CTCTCTCCAA AGCTGAGTTG TGACACAAGA 
CCTCTCATTC TGAAGACACT GAGTGAACTA TTTTCTCTAG TTCCTTCCTT AACAGTCAAT 
ACAACTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAC TCATACTCAA 
AACAAGGACC CAATTGTGGC AAATGCTGCA TATAGATCCC TGGCCAATTT TAGTGCAGGA 
GAACACACCA TTCTTCATCT GCCTGAAAAG ATAAGACCAG AAATTCCCAT TCCTGAAGAG 
TTAGATGATG ATGAAGATGA TGAGGATGTG GATCTTTCAG TTCCTGGCTC TTGCTATCTC 
AAACTGTTGT CACTCACTCC CCCTTTGGTT TTACCAGCTT TGGAGGAATT TTTTACATCA 
CTTGTGAAGC AAGAAATGGT GAATATGCCT CGTGGGATAT ATCACTCTGC ATTAAAAGGA 
GGTGTCCGCT CAGACCAAGG AAAGACTGTA GCAGGAATCC CCAATTTTAT ATTGAAAATG 
TATGAAACAA ACAAGCAACC AGGATTGAAA CCTGGCCTTG CAGGTGGTAT GTTATTTTGC 
TATGATGTTT CCATGTATCA GAGTAAAGAT GGAAAACCAT TGAATAGACT GATGGCCAGC 
AGAGGGCGAA GTTTCAAGCA GACATCACTT GCTCTTGTAC ATGAGGTTCA TATCCAGCTT 
TCAGAGTGGC ACCGTGCGAT TTTTCTTCCA CAGGCCTGGC TTGCATACAT GAATCGAGCT 
TATCATGCCA TTTTACAGGG AAGACTAGGA GAGCTGGAGT TGCAGTTAAA ACATGGAAAA 
GAAGAACCTG AGGAGGTGCA GTACAAAAAA AGCACAGCTT GGCTCTGGGT TAGAGACATG 
CTGACTGATG AGATCACCAA GGCAGCTGCA AAGGAGAGTC CGGTAGTGAA AGGCAATGCG 
CTGTTAGCTC TAAGCAGCCT TGCTGTCGTC GTATCTAGAC ATGAAGCCAG CGTCTCCTCA 
GACTCTGACG GGCTCCTGGA GGTTCAACCT AATTTCCTTT CAATGAAAGA GTGGGTTTCC 
ATGATACTTG ATACACTCTT GGTCATTGTG GATAGCCATT ACCAACCCAG AGGGCAACTT 
CTCTCCTGGT TTTATTATAA GTCCTATTCT GGTGAAAACA CAGCTAGTGC CATTGCCCGT 
TCTGCTGCCG CCACGGCTTT GTCTCTCCTT GTGCCAGTTT TCATTATCTC TTGCAAAGAG 
AAGGTTGAGG AAATCCTGAA CATGCTGACT GCCAGGTTAC CTGGGAAACC AAGTGCTGAT 
GAGTCTCAAG CCGTGCAAAT CCACATGGGC CTTGCTTTAG GGATGTTTCT CTCTCGCTTG 
TGTGAAGAGA AACTCAGTGA TATATGTGGC CAAGAGATGA ACCTTCTTCT GATGAAGTCG 
TTGGATGCCC TGGAAAATTG CTGCTTTGAC ACTAGTCTTG AATACAACAC GGGCTGTATA 
TTGGGAGTTG GACTTGTTCT GTCCCTCATG AGTCACAGCA GCCAAATGCA GTCCTGCGTT 
CACGTGACAG CATTGCTTCG GAAGCTGTCT GCATACCTAG ATGACAGTGG GAGCCAGAGC 
AGAACGTTTC AGGAGGTCCT TGCCTACACA CTTAGCTGTG TATGTACATC AGCGTTCAGT 
GCTGGAATTA TTGAGGCTAC AGAGGCTGAG GATGTTATGA ACAAGCTTCG ACTGTTAGTG 
GAGAATAGCC AGCAGACTTC AGGTTTTGCC CTGGCTTTAG GAAACGTAGT TCATGGATTG 
TCTGTGTGTG GACATGGAAA AGCTGAAGAC TTGGGCAGCA AACTACTCCC TGCCTGGATC 
AGAATTGTTC TAACAGAGGG CACTCCCACA ATGCTTTGTC TGGCAGCTCT TCATGGCATG 
GTGGCCCTGG TAGGCTCTGA AGGGGATGTA ATGCAGCTGA AATCAGAAGC CATCCAGACC 
TCTCATTTTC AAGGCAGACT TAATGAAGTC ATTAGAACCT TAACTCAGGT CATTAGTGTC 
TCTGGGGTGA TTGGTCTCCA GTCAAATGCA GTCTGGCTTC TTGGACATCT TCATCTATCT 
ACTCTATCCT CAAGTCAAAG TAGAGCCTCT GTTCCTACTG ACTATAGCTA TTTGCCTGAA 
AGCAGTTTTA TTGGAGCAGC TATTGGCTTC TTCATTACAG GAGGAAAAAA AGGTCCTGAA 
TCTGTGCCTC CTTCCCTTCT TAAAGTAGTG ATGAAACCCA TAGCAACTGT TGGAGAAAGC 
TACCAATATC CTCCTGTGAA CTGGGCTGCA CTTCTCTCTC CACTTATGAG GCTAAATTTT 
GGTGAAGAGA TCCAGCAACT GTGCCTTGAA ATTATGGTGA CCCAGGCACA GTCATCCCAG 
AATGCAGCTG CACTATTGGG CTTGTGGGTG ACACCACCAC TGATCCACAG TCTGAGTCTG 
AATACCAAGA GATATCTCCT GATATCTGCA CCTCTGTGGA TAAAACACAT CTCTGATGAA 
CAGATCCTGG GTTTTGTTGA AAATTTAATG GTGGCAGTTT TTAAAGCAGC TTCCCCACTT 
GGAAATCCTG AGCTATGCCC GAGTGCTTTA CAGGGTCTGA GCCAGGCCAT GAAACTGCCC 
AGCCCTGCTC ACCACCTCTG GAGTCTGCTC TCTGAAGCTA CTGGGAAAAT TTTTGACCTC 
CTGCCAAATA AGATTCGGAG AAAGGATCTA GAGCTGTATA TCAGCATAGC AAAATGCCTC 
TTAGAAATGA CAGATGATGA TGCCAATCGG ATCGCCCAGG TTACTAAGAG CAACATAGAA 
AAGGCTGCCT TTGTCAAACT GTACTTAGTC TCTCAAGGAC GATTCCCCTT GGTGAACCTG 
ACCGATATGC TGAGTGTTGC TGTGCAGCAC CGTGAGAAAG AGGTGTTGGC CTGGATGATT 
CTGCACAGCT TATACCAGGC ACGGATTGTG AGCCATGCCA ACACGGGCGT TTTGAAGAGA 
ATGGAGTGGC TCTTGGAACT GATGGGTTAT ATTAGAAATG TTGCGTACCA GTCAACATCC 
TTTCAGAATA CGGCTCTTGA TGAGGCTTTG GACTTCTTCT TGCTGATATT TGCAACCGCA 
GTGGTTGCAT GGGCCGACCA CACTGCCCCT CTCCTCCTTG GTCTCAGTGC CAGTTGGTTG 
CCATGGCATC AGGAGAATGG CCCGGCTGGG CCAGTACCAA GCTTCCTTGG CAGGAGTCCA 
ATGCACAGGG TCACTGTGCA GGAGGTTCTC ACTCTCCTTC CCAATAGCAT GGCTCTGCTG 
CTGCAGAAAG AGCCATGGAA GGAAGAGACC CAGAAGTTCA TTGACTGGCT ATTCAGCATC 
ATGGAAAGCC CTAAAGAAGC CCTCTCAGCA AAGTCCAGGG ATCTTTTGAA AGCCACCCTG 
CTGTCCTTGA GAGTTCTCCC AGAGTTTAAG AAGAAAGCTG TATGGACCAG AGCGTATGGT 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FOCAD ( XM_009244394.2 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009242669.2 Pongo abelii focadhesin (FOCAD), transcript variant X9, mRNA.

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CloneID	OSa186756
Clone ID Related Accession (Same CDS sequence)	XM_009244392.2 , XM_024252331.1 , XM_024252332.1 , XM_024252334.1 , XM_009244394.2
Accession Version	XM_009244394.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	focadhesin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036912.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 28, 2018 this sequence version replaced XM_009244394.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGATG ATATCAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA ATCACAGGCT 
GCGGGTCATC TTATTGCTGC AGTCCTAAAG GAGAATGGTT TTTCAGAAAA GATTTACCAA 
TCTACAAATC AGACTCCTGC TTTGAACTTG CTGTGGGAGA AGTGTTGCAG TGACAATGTA 
GTGGTTCGAA CAGCCTGCTG TGAAGGTCTG GTGGCACTCG TTGCTCAGGA TCATGCAGAG 
TTCAGCTATG TTCTCAATGG GATACTCAAC TTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCATTATGAA ATTACTACAA ATGCAAGCTC TTAAGGAAGG ACAAGGTGGG 
GAAAAGAATA TTCAGAGTGT ATATACCATT AGAAATCATC CTCATCCTTT GATCACTGTG 
CTTGAACACA GACCTGATTG TTGGCCAGTG TTTTTGCAGC AGCTGACAGC ATTTTTCCAG 
CAGTGCCCTG AAAGGTCAGA AGTTTCATGC ATTCAAATAA TGGCACCATT TCTGTGGTAT 
CTGTATTGTG AACCATCTCA GTTACAAGAA TATGCTAAAC TCCGACTAGC CCTGCTGAAA 
GTCTTACTTC AACCCCAGGT TCTTTGTGAC AAGGATCAAC CATCAATACT GCAACAGCAG 
ATACTTCAAC TGTGTTGTGA CATAGTTCCA TGTTTGCAGA TAAAAGATTT GATACAGACA 
ACAGAAGCGA TGATGTTTAT TGAGGAAATA TATTTAAGCC TTTTGCGTCA TCCTGTTTTC 
TGGAAAATTC AGCTTACCCA GATGACTCTT CAGCTGCTGT GTGTCGGTGA AGTCAGCTTA 
AAGATAACTG GTGAATGTTC ATCTTCAATT CACCTCTTAG AGCACAGTGT TGAACTTCTG 
AAGGAGGATT TTCCTGTTGA ACTGGTCATA ATTGGAATAG CTTTACTACT TCTACAGACT 
CCAGCAAGTC AGCAGAAGCC AATCTTAAAT CTAGCTTTGA AGCTCTTCTC TGTTACTGAG 
GATCAGAAAA TCCCAAAGTC CTCTCTGCTG CTAGTGATGC CAATTCTGCA GATACTGTCT 
TCTACTGCCT TGGAAGACTG TATATCTGTG GATGAAGAAG GTCCCTCTAG GCAGCAGTTG 
GCTCTAAACC TTTTGGAAAT GATACAGCAG GAATGTTACA GAGATGACCA CCAAAAGCTC 
TCCTACAAGC TTGTGTGCCC TGTAACCAGT ATGTATGGTT CAGTATTTAC AGCCTGGAGG 
ATTCTTGAAG TAATGACAGA CTCCTCTGCT GCAAGTGACT GGTTGGCTTC AGTAGAGTCA 
TTGCTTCCTA TTACTACTGT GATCCCTGTG CCTGCCTTTC TTCTGCTGGC TCACCTCCTT 
GTTGAAGACA AAGGACAAAA TCTTCACCAA ATACTCAAGG TCACTACAGA ATTAGCCCAA 
GCAGATTCCT CCCAGGTGCC AAATCTGATT CCAGTTTTGA TGTTCAAATT GGGAAGACCA 
CTGGAACCTA TATTATATAA TGATATATTG TATACTTTAC CTAAGCTTGG TGTTCACAAG 
GTGTGTATAG GACAAATTCT ACGAGTAATA CAACTGCTTG GAACCACACC ACGACTAAGA 
GCTGTCACTT TGCGCTTGCT GACATCTTTG TGGGAAAAGC AGGACCGAGT CTATCCTGAA 
CTGCAGCGTT TCATGGCTGT GTCTGATGTA CCTTCTCTTT CGGTGGGCAA GGAAGTCCAA 
TGGGAGAAAC TGATTGCAAA AGCAGCATCA ATCAGAGATA TATGTAAGCA GAGGCCATAT 
CAACATGGTG CAGATATGCT GGCAGCTATT TCTCAGGTGT TGAATGAATG CACCAAGCCT 
GATCAAGCTA CTCCAGCAGC CTTGGTATTA CAAGGTCTTC ATGCACTCTG TCAAGCTGAG 
GTTGTTTGCA TTCGCTCCAC TTGGAATGCT CTCTCTCCAA AGCTGAGTTG TGACACAAGA 
CCTCTCATTC TGAAGACACT GAGTGAACTA TTTTCTCTAG TTCCTTCCTT AACAGTCAAT 
ACAACTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAC TCATACTCAA 
AACAAGGACC CAATTGTGGC AAATGCTGCA TATAGATCCC TGGCCAATTT TAGTGCAGGA 
GAACACACCA TTCTTCATCT GCCTGAAAAG ATAAGACCAG AAATTCCCAT TCCTGAAGAG 
TTAGATGATG ATGAAGATGA TGAGGATGTG GATCTTTCAG TTCCTGGCTC TTGCTATCTC 
AAACTGTTGT CACTCACTCC CCCTTTGGTT TTACCAGCTT TGGAGGAATT TTTTACATCA 
CTTGTGAAGC AAGAAATGGT GAATATGCCT CGTGGGATAT ATCACTCTGC ATTAAAAGGA 
GGTGTCCGCT CAGACCAAGG AAAGACTGTA GCAGGAATCC CCAATTTTAT ATTGAAAATG 
TATGAAACAA ACAAGCAACC AGGATTGAAA CCTGGCCTTG CAGGTGGTAT GTTATTTTGC 
TATGATGTTT CCATGTATCA GAGTAAAGAT GGAAAACCAT TGAATAGACT GATGGCCAGC 
AGAGGGCGAA GTTTCAAGCA GACATCACTT GCTCTTGTAC ATGAGGTTCA TATCCAGCTT 
TCAGAGTGGC ACCGTGCGAT TTTTCTTCCA CAGGCCTGGC TTGCATACAT GAATCGAGCT 
TATCATGCCA TTTTACAGGG AAGACTAGGA GAGCTGGAGT TGCAGTTAAA ACATGGAAAA 
GAAGAACCTG AGGAGGTGCA GTACAAAAAA AGCACAGCTT GGCTCTGGGT TAGAGACATG 
CTGACTGATG AGATCACCAA GGCAGCTGCA AAGGAGAGTC CGGTAGTGAA AGGCAATGCG 
CTGTTAGCTC TAAGCAGCCT TGCTGTCGTC GTATCTAGAC ATGAAGCCAG CGTCTCCTCA 
GACTCTGACG GGCTCCTGGA GGTTCAACCT AATTTCCTTT CAATGAAAGA GTGGGTTTCC 
ATGATACTTG ATACACTCTT GGTCATTGTG GATAGCCATT ACCAACCCAG AGGGCAACTT 
CTCTCCTGGT TTTATTATAA GTCCTATTCT GGTGAAAACA CAGCTAGTGC CATTGCCCGT 
TCTGCTGCCG CCACGGCTTT GTCTCTCCTT GTGCCAGTTT TCATTATCTC TTGCAAAGAG 
AAGGTTGAGG AAATCCTGAA CATGCTGACT GCCAGGTTAC CTGGGAAACC AAGTGCTGAT 
GAGTCTCAAG CCGTGCAAAT CCACATGGGC CTTGCTTTAG GGATGTTTCT CTCTCGCTTG 
TGTGAAGAGA AACTCAGTGA TATATGTGGC CAAGAGATGA ACCTTCTTCT GATGAAGTCG 
TTGGATGCCC TGGAAAATTG CTGCTTTGAC ACTAGTCTTG AATACAACAC GGGCTGTATA 
TTGGGAGTTG GACTTGTTCT GTCCCTCATG AGTCACAGCA GCCAAATGCA GTCCTGCGTT 
CACGTGACAG CATTGCTTCG GAAGCTGTCT GCATACCTAG ATGACAGTGG GAGCCAGAGC 
AGAACGTTTC AGGAGGTCCT TGCCTACACA CTTAGCTGTG TATGTACATC AGCGTTCAGT 
GCTGGAATTA TTGAGGCTAC AGAGGCTGAG GATGTTATGA ACAAGCTTCG ACTGTTAGTG 
GAGAATAGCC AGCAGACTTC AGGTTTTGCC CTGGCTTTAG GAAACGTAGT TCATGGATTG 
TCTGTGTGTG GACATGGAAA AGCTGAAGAC TTGGGCAGCA AACTACTCCC TGCCTGGATC 
AGAATTGTTC TAACAGAGGG CACTCCCACA ATGCTTTGTC TGGCAGCTCT TCATGGCATG 
GTGGCCCTGG TAGGCTCTGA AGGGGATGTA ATGCAGCTGA AATCAGAAGC CATCCAGACC 
TCTCATTTTC AAGGCAGACT TAATGAAGTC ATTAGAACCT TAACTCAGGT CATTAGTGTC 
TCTGGGGTGA TTGGTCTCCA GTCAAATGCA GTCTGGCTTC TTGGACATCT TCATCTATCT 
ACTCTATCCT CAAGTCAAAG TAGAGCCTCT GTTCCTACTG ACTATAGCTA TTTGCCTGAA 
AGCAGTTTTA TTGGAGCAGC TATTGGCTTC TTCATTACAG GAGGAAAAAA AGGTCCTGAA 
TCTGTGCCTC CTTCCCTTCT TAAAGTAGTG ATGAAACCCA TAGCAACTGT TGGAGAAAGC 
TACCAATATC CTCCTGTGAA CTGGGCTGCA CTTCTCTCTC CACTTATGAG GCTAAATTTT 
GGTGAAGAGA TCCAGCAACT GTGCCTTGAA ATTATGGTGA CCCAGGCACA GTCATCCCAG 
AATGCAGCTG CACTATTGGG CTTGTGGGTG ACACCACCAC TGATCCACAG TCTGAGTCTG 
AATACCAAGA GATATCTCCT GATATCTGCA CCTCTGTGGA TAAAACACAT CTCTGATGAA 
CAGATCCTGG GTTTTGTTGA AAATTTAATG GTGGCAGTTT TTAAAGCAGC TTCCCCACTT 
GGAAATCCTG AGCTATGCCC GAGTGCTTTA CAGGGTCTGA GCCAGGCCAT GAAACTGCCC 
AGCCCTGCTC ACCACCTCTG GAGTCTGCTC TCTGAAGCTA CTGGGAAAAT TTTTGACCTC 
CTGCCAAATA AGATTCGGAG AAAGGATCTA GAGCTGTATA TCAGCATAGC AAAATGCCTC 
TTAGAAATGA CAGATGATGA TGCCAATCGG ATCGCCCAGG TTACTAAGAG CAACATAGAA 
AAGGCTGCCT TTGTCAAACT GTACTTAGTC TCTCAAGGAC GATTCCCCTT GGTGAACCTG 
ACCGATATGC TGAGTGTTGC TGTGCAGCAC CGTGAGAAAG AGGTGTTGGC CTGGATGATT 
CTGCACAGCT TATACCAGGC ACGGATTGTG AGCCATGCCA ACACGGGCGT TTTGAAGAGA 
ATGGAGTGGC TCTTGGAACT GATGGGTTAT ATTAGAAATG TTGCGTACCA GTCAACATCC 
TTTCAGAATA CGGCTCTTGA TGAGGCTTTG GACTTCTTCT TGCTGATATT TGCAACCGCA 
GTGGTTGCAT GGGCCGACCA CACTGCCCCT CTCCTCCTTG GTCTCAGTGC CAGTTGGTTG 
CCATGGCATC AGGAGAATGG CCCGGCTGGG CCAGTACCAA GCTTCCTTGG CAGGAGTCCA 
ATGCACAGGG TCACTGTGCA GGAGGTTCTC ACTCTCCTTC CCAATAGCAT GGCTCTGCTG 
CTGCAGAAAG AGCCATGGAA GGAAGAGACC CAGAAGTTCA TTGACTGGCT ATTCAGCATC 
ATGGAAAGCC CTAAAGAAGC CCTCTCAGCA AAGTCCAGGG ATCTTTTGAA AGCCACCCTG 
CTGTCCTTGA GAGTTCTCCC AGAGTTTAAG AAGAAAGCTG TATGGACCAG AGCGTATGGT 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009242669.2
CDS	105..5510
Translation	MSDDIRKRFEFPNSLIQSQAAGHLIAAVLKENGFSEKIYQSTNQTPALNLLWEKCCSDNVVVRTACCEGLVALVAQDHAEFSYVLNGILNLIPSTRNTHGLIKAIMKLLQMQALKEGQGGEKNIQSVYTIRNHPHPLITVLEHRPDCWPVFLQQLTAFFQQCPERSEVSCIQIMAPFLWYLYCEPSQLQEYAKLRLALLKVLLQPQVLCDKDQPSILQQQILQLCCDIVPCLQIKDLIQTTEAMMFIEEIYLSLLRHPVFWKIQLTQMTLQLLCVGEVSLKITGECSSSIHLLEHSVELLKEDFPVELVIIGIALLLLQTPASQQKPILNLALKLFSVTEDQKIPKSSLLLVMPILQILSSTALEDCISVDEEGPSRQQLALNLLEMIQQECYRDDHQKLSYKLVCPVTSMYGSVFTAWRILEVMTDSSAASDWLASVESLLPITTVIPVPAFLLLAHLLVEDKGQNLHQILKVTTELAQADSSQVPNLIPVLMFKLGRPLEPILYNDILYTLPKLGVHKVCIGQILRVIQLLGTTPRLRAVTLRLLTSLWEKQDRVYPELQRFMAVSDVPSLSVGKEVQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQATPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDTRPLILKTLSELFSLVPSLTVNTTEYENFKVQVLSFLWTHTQNKDPIVANAAYRSLANFSAGEHTILHLPEKIRPEIPIPEELDDDEDDEDVDLSVPGSCYLKLLSLTPPLVLPALEEFFTSLVKQEMVNMPRGIYHSALKGGVRSDQGKTVAGIPNFILKMYETNKQPGLKPGLAGGMLFCYDVSMYQSKDGKPLNRLMASRGRSFKQTSLALVHEVHIQLSEWHRAIFLPQAWLAYMNRAYHAILQGRLGELELQLKHGKEEPEEVQYKKSTAWLWVRDMLTDEITKAAAKESPVVKGNALLALSSLAVVVSRHEASVSSDSDGLLEVQPNFLSMKEWVSMILDTLLVIVDSHYQPRGQLLSWFYYKSYSGENTASAIARSAAATALSLLVPVFIISCKEKVEEILNMLTARLPGKPSADESQAVQIHMGLALGMFLSRLCEEKLSDICGQEMNLLLMKSLDALENCCFDTSLEYNTGCILGVGLVLSLMSHSSQMQSCVHVTALLRKLSAYLDDSGSQSRTFQEVLAYTLSCVCTSAFSAGIIEATEAEDVMNKLRLLVENSQQTSGFALALGNVVHGLSVCGHGKAEDLGSKLLPAWIRIVLTEGTPTMLCLAALHGMVALVGSEGDVMQLKSEAIQTSHFQGRLNEVIRTLTQVISVSGVIGLQSNAVWLLGHLHLSTLSSSQSRASVPTDYSYLPESSFIGAAIGFFITGGKKGPESVPPSLLKVVMKPIATVGESYQYPPVNWAALLSPLMRLNFGEEIQQLCLEIMVTQAQSSQNAAALLGLWVTPPLIHSLSLNTKRYLLISAPLWIKHISDEQILGFVENLMVAVFKAASPLGNPELCPSALQGLSQAMKLPSPAHHLWSLLSEATGKIFDLLPNKIRRKDLELYISIAKCLLEMTDDDANRIAQVTKSNIEKAAFVKLYLVSQGRFPLVNLTDMLSVAVQHREKEVLAWMILHSLYQARIVSHANTGVLKRMEWLLELMGYIRNVAYQSTSFQNTALDEALDFFLLIFATAVVAWADHTAPLLLGLSASWLPWHQENGPAGPVPSFLGRSPMHRVTVQEVLTLLPNSMALLLQKEPWKEETQKFIDWLFSIMESPKEALSAKSRDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_009244394.2
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii focadhesin (FOCAD), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009244394.2

ORF Insert Sequence:

ATGTCAGATG ATATCAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA ATCACAGGCT 
GCGGGTCATC TTATTGCTGC AGTCCTAAAG GAGAATGGTT TTTCAGAAAA GATTTACCAA 
TCTACAAATC AGACTCCTGC TTTGAACTTG CTGTGGGAGA AGTGTTGCAG TGACAATGTA 
GTGGTTCGAA CAGCCTGCTG TGAAGGTCTG GTGGCACTCG TTGCTCAGGA TCATGCAGAG 
TTCAGCTATG TTCTCAATGG GATACTCAAC TTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCATTATGAA ATTACTACAA ATGCAAGCTC TTAAGGAAGG ACAAGGTGGG 
GAAAAGAATA TTCAGAGTGT ATATACCATT AGAAATCATC CTCATCCTTT GATCACTGTG 
CTTGAACACA GACCTGATTG TTGGCCAGTG TTTTTGCAGC AGCTGACAGC ATTTTTCCAG 
CAGTGCCCTG AAAGGTCAGA AGTTTCATGC ATTCAAATAA TGGCACCATT TCTGTGGTAT 
CTGTATTGTG AACCATCTCA GTTACAAGAA TATGCTAAAC TCCGACTAGC CCTGCTGAAA 
GTCTTACTTC AACCCCAGGT TCTTTGTGAC AAGGATCAAC CATCAATACT GCAACAGCAG 
ATACTTCAAC TGTGTTGTGA CATAGTTCCA TGTTTGCAGA TAAAAGATTT GATACAGACA 
ACAGAAGCGA TGATGTTTAT TGAGGAAATA TATTTAAGCC TTTTGCGTCA TCCTGTTTTC 
TGGAAAATTC AGCTTACCCA GATGACTCTT CAGCTGCTGT GTGTCGGTGA AGTCAGCTTA 
AAGATAACTG GTGAATGTTC ATCTTCAATT CACCTCTTAG AGCACAGTGT TGAACTTCTG 
AAGGAGGATT TTCCTGTTGA ACTGGTCATA ATTGGAATAG CTTTACTACT TCTACAGACT 
CCAGCAAGTC AGCAGAAGCC AATCTTAAAT CTAGCTTTGA AGCTCTTCTC TGTTACTGAG 
GATCAGAAAA TCCCAAAGTC CTCTCTGCTG CTAGTGATGC CAATTCTGCA GATACTGTCT 
TCTACTGCCT TGGAAGACTG TATATCTGTG GATGAAGAAG GTCCCTCTAG GCAGCAGTTG 
GCTCTAAACC TTTTGGAAAT GATACAGCAG GAATGTTACA GAGATGACCA CCAAAAGCTC 
TCCTACAAGC TTGTGTGCCC TGTAACCAGT ATGTATGGTT CAGTATTTAC AGCCTGGAGG 
ATTCTTGAAG TAATGACAGA CTCCTCTGCT GCAAGTGACT GGTTGGCTTC AGTAGAGTCA 
TTGCTTCCTA TTACTACTGT GATCCCTGTG CCTGCCTTTC TTCTGCTGGC TCACCTCCTT 
GTTGAAGACA AAGGACAAAA TCTTCACCAA ATACTCAAGG TCACTACAGA ATTAGCCCAA 
GCAGATTCCT CCCAGGTGCC AAATCTGATT CCAGTTTTGA TGTTCAAATT GGGAAGACCA 
CTGGAACCTA TATTATATAA TGATATATTG TATACTTTAC CTAAGCTTGG TGTTCACAAG 
GTGTGTATAG GACAAATTCT ACGAGTAATA CAACTGCTTG GAACCACACC ACGACTAAGA 
GCTGTCACTT TGCGCTTGCT GACATCTTTG TGGGAAAAGC AGGACCGAGT CTATCCTGAA 
CTGCAGCGTT TCATGGCTGT GTCTGATGTA CCTTCTCTTT CGGTGGGCAA GGAAGTCCAA 
TGGGAGAAAC TGATTGCAAA AGCAGCATCA ATCAGAGATA TATGTAAGCA GAGGCCATAT 
CAACATGGTG CAGATATGCT GGCAGCTATT TCTCAGGTGT TGAATGAATG CACCAAGCCT 
GATCAAGCTA CTCCAGCAGC CTTGGTATTA CAAGGTCTTC ATGCACTCTG TCAAGCTGAG 
GTTGTTTGCA TTCGCTCCAC TTGGAATGCT CTCTCTCCAA AGCTGAGTTG TGACACAAGA 
CCTCTCATTC TGAAGACACT GAGTGAACTA TTTTCTCTAG TTCCTTCCTT AACAGTCAAT 
ACAACTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAC TCATACTCAA 
AACAAGGACC CAATTGTGGC AAATGCTGCA TATAGATCCC TGGCCAATTT TAGTGCAGGA 
GAACACACCA TTCTTCATCT GCCTGAAAAG ATAAGACCAG AAATTCCCAT TCCTGAAGAG 
TTAGATGATG ATGAAGATGA TGAGGATGTG GATCTTTCAG TTCCTGGCTC TTGCTATCTC 
AAACTGTTGT CACTCACTCC CCCTTTGGTT TTACCAGCTT TGGAGGAATT TTTTACATCA 
CTTGTGAAGC AAGAAATGGT GAATATGCCT CGTGGGATAT ATCACTCTGC ATTAAAAGGA 
GGTGTCCGCT CAGACCAAGG AAAGACTGTA GCAGGAATCC CCAATTTTAT ATTGAAAATG 
TATGAAACAA ACAAGCAACC AGGATTGAAA CCTGGCCTTG CAGGTGGTAT GTTATTTTGC 
TATGATGTTT CCATGTATCA GAGTAAAGAT GGAAAACCAT TGAATAGACT GATGGCCAGC 
AGAGGGCGAA GTTTCAAGCA GACATCACTT GCTCTTGTAC ATGAGGTTCA TATCCAGCTT 
TCAGAGTGGC ACCGTGCGAT TTTTCTTCCA CAGGCCTGGC TTGCATACAT GAATCGAGCT 
TATCATGCCA TTTTACAGGG AAGACTAGGA GAGCTGGAGT TGCAGTTAAA ACATGGAAAA 
GAAGAACCTG AGGAGGTGCA GTACAAAAAA AGCACAGCTT GGCTCTGGGT TAGAGACATG 
CTGACTGATG AGATCACCAA GGCAGCTGCA AAGGAGAGTC CGGTAGTGAA AGGCAATGCG 
CTGTTAGCTC TAAGCAGCCT TGCTGTCGTC GTATCTAGAC ATGAAGCCAG CGTCTCCTCA 
GACTCTGACG GGCTCCTGGA GGTTCAACCT AATTTCCTTT CAATGAAAGA GTGGGTTTCC 
ATGATACTTG ATACACTCTT GGTCATTGTG GATAGCCATT ACCAACCCAG AGGGCAACTT 
CTCTCCTGGT TTTATTATAA GTCCTATTCT GGTGAAAACA CAGCTAGTGC CATTGCCCGT 
TCTGCTGCCG CCACGGCTTT GTCTCTCCTT GTGCCAGTTT TCATTATCTC TTGCAAAGAG 
AAGGTTGAGG AAATCCTGAA CATGCTGACT GCCAGGTTAC CTGGGAAACC AAGTGCTGAT 
GAGTCTCAAG CCGTGCAAAT CCACATGGGC CTTGCTTTAG GGATGTTTCT CTCTCGCTTG 
TGTGAAGAGA AACTCAGTGA TATATGTGGC CAAGAGATGA ACCTTCTTCT GATGAAGTCG 
TTGGATGCCC TGGAAAATTG CTGCTTTGAC ACTAGTCTTG AATACAACAC GGGCTGTATA 
TTGGGAGTTG GACTTGTTCT GTCCCTCATG AGTCACAGCA GCCAAATGCA GTCCTGCGTT 
CACGTGACAG CATTGCTTCG GAAGCTGTCT GCATACCTAG ATGACAGTGG GAGCCAGAGC 
AGAACGTTTC AGGAGGTCCT TGCCTACACA CTTAGCTGTG TATGTACATC AGCGTTCAGT 
GCTGGAATTA TTGAGGCTAC AGAGGCTGAG GATGTTATGA ACAAGCTTCG ACTGTTAGTG 
GAGAATAGCC AGCAGACTTC AGGTTTTGCC CTGGCTTTAG GAAACGTAGT TCATGGATTG 
TCTGTGTGTG GACATGGAAA AGCTGAAGAC TTGGGCAGCA AACTACTCCC TGCCTGGATC 
AGAATTGTTC TAACAGAGGG CACTCCCACA ATGCTTTGTC TGGCAGCTCT TCATGGCATG 
GTGGCCCTGG TAGGCTCTGA AGGGGATGTA ATGCAGCTGA AATCAGAAGC CATCCAGACC 
TCTCATTTTC AAGGCAGACT TAATGAAGTC ATTAGAACCT TAACTCAGGT CATTAGTGTC 
TCTGGGGTGA TTGGTCTCCA GTCAAATGCA GTCTGGCTTC TTGGACATCT TCATCTATCT 
ACTCTATCCT CAAGTCAAAG TAGAGCCTCT GTTCCTACTG ACTATAGCTA TTTGCCTGAA 
AGCAGTTTTA TTGGAGCAGC TATTGGCTTC TTCATTACAG GAGGAAAAAA AGGTCCTGAA 
TCTGTGCCTC CTTCCCTTCT TAAAGTAGTG ATGAAACCCA TAGCAACTGT TGGAGAAAGC 
TACCAATATC CTCCTGTGAA CTGGGCTGCA CTTCTCTCTC CACTTATGAG GCTAAATTTT 
GGTGAAGAGA TCCAGCAACT GTGCCTTGAA ATTATGGTGA CCCAGGCACA GTCATCCCAG 
AATGCAGCTG CACTATTGGG CTTGTGGGTG ACACCACCAC TGATCCACAG TCTGAGTCTG 
AATACCAAGA GATATCTCCT GATATCTGCA CCTCTGTGGA TAAAACACAT CTCTGATGAA 
CAGATCCTGG GTTTTGTTGA AAATTTAATG GTGGCAGTTT TTAAAGCAGC TTCCCCACTT 
GGAAATCCTG AGCTATGCCC GAGTGCTTTA CAGGGTCTGA GCCAGGCCAT GAAACTGCCC 
AGCCCTGCTC ACCACCTCTG GAGTCTGCTC TCTGAAGCTA CTGGGAAAAT TTTTGACCTC 
CTGCCAAATA AGATTCGGAG AAAGGATCTA GAGCTGTATA TCAGCATAGC AAAATGCCTC 
TTAGAAATGA CAGATGATGA TGCCAATCGG ATCGCCCAGG TTACTAAGAG CAACATAGAA 
AAGGCTGCCT TTGTCAAACT GTACTTAGTC TCTCAAGGAC GATTCCCCTT GGTGAACCTG 
ACCGATATGC TGAGTGTTGC TGTGCAGCAC CGTGAGAAAG AGGTGTTGGC CTGGATGATT 
CTGCACAGCT TATACCAGGC ACGGATTGTG AGCCATGCCA ACACGGGCGT TTTGAAGAGA 
ATGGAGTGGC TCTTGGAACT GATGGGTTAT ATTAGAAATG TTGCGTACCA GTCAACATCC 
TTTCAGAATA CGGCTCTTGA TGAGGCTTTG GACTTCTTCT TGCTGATATT TGCAACCGCA 
GTGGTTGCAT GGGCCGACCA CACTGCCCCT CTCCTCCTTG GTCTCAGTGC CAGTTGGTTG 
CCATGGCATC AGGAGAATGG CCCGGCTGGG CCAGTACCAA GCTTCCTTGG CAGGAGTCCA 
ATGCACAGGG TCACTGTGCA GGAGGTTCTC ACTCTCCTTC CCAATAGCAT GGCTCTGCTG 
CTGCAGAAAG AGCCATGGAA GGAAGAGACC CAGAAGTTCA TTGACTGGCT ATTCAGCATC 
ATGGAAAGCC CTAAAGAAGC CCTCTCAGCA AAGTCCAGGG ATCTTTTGAA AGCCACCCTG 
CTGTCCTTGA GAGTTCTCCC AGAGTTTAAG AAGAAAGCTG TATGGACCAG AGCGTATGGT 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FOCAD ( XM_024252335.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024108103.1 Pongo abelii focadhesin (FOCAD), transcript variant X10, mRNA.

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CloneID	OSa186757
Clone ID Related Accession (Same CDS sequence)	XM_024252335.1
Accession Version	XM_024252335.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	focadhesin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036912.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGATG ATATCAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA ATCACAGGCT 
GCGGGTCATC TTATTGCTGC AGTCCTAAAG GAGAATGGTT TTTCAGAAAA GATTTACCAA 
TCTACAAATC AGACTCCTGC TTTGAACTTG CTGTGGGAGA AGTGTTGCAG TGACAATGTA 
GTGGTTCGAA CAGCCTGCTG TGAAGGTCTG GTGGCACTCG TTGCTCAGGA TCATGCAGAG 
TTCAGCTATG TTCTCAATGG GATACTCAAC TTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCATTATGAA ATTACTACAA ATGCAAGCTC TTAAGGAAGG ACAAGGTGGG 
GAAAAGAATA TTCAGAGTGT ATATACCATT AGAAATCATC CTCATCCTTT GATCACTGTG 
CTTGAACACA GACCTGATTG TTGGCCAGTG TTTTTGCAGC AGCTGACAGC ATTTTTCCAG 
CAGTGCCCTG AAAGGTCAGA AGTTTCATGC ATTCAAATAA TGGCACCATT TCTGTGGTAT 
CTGTATTGTG AACCATCTCA GTTACAAGAA TATGCTAAAC TCCGACTAGC CCTGCTGAAA 
GTCTTACTTC AACCCCAGGT TCTTTGTGAC AAGGATCAAC CATCAATACT GCAACAGCAG 
ATACTTCAAC TGTGTTGTGA CATAGTTCCA TGTTTGCAGA TAAAAGATTT GATACAGACA 
ACAGAAGCGA TGATGTTTAT TGAGGAAATA TATTTAAGCC TTTTGCGTCA TCCTGTTTTC 
TGGAAAATTC AGCTTACCCA GATGACTCTT CAGCTGCTGT GTGTCGGTGA AGTCAGCTTA 
AAGATAACTG GTGAATGTTC ATCTTCAATT CACCTCTTAG AGCACAGTGT TGAACTTCTG 
AAGGAGGATT TTCCTGTTGA ACTGGTCATA ATTGGAATAG CTTTACTACT TCTACAGACT 
CCAGCAAGTC AGCAGAAGCC AATCTTAAAT CTAGCTTTGA AGCTCTTCTC TGTTACTGAG 
GATCAGAAAA TCCCAAAGTC CTCTCTGCTG CTAGTGATGC CAATTCTGCA GATACTGTCT 
TCTACTGCCT TGGAAGACTG TATATCTGTG GATGAAGAAG GTCCCTCTAG GCAGCAGTTG 
GCTCTAAACC TTTTGGAAAT GATACAGCAG GAATGTTACA GAGATGACCA CCAAAAGCTC 
TCCTACAAGC TTGTGTGCCC TGTAACCAGT ATGTATGGTT CAGTATTTAC AGCCTGGAGG 
ATTCTTGAAG TAATGACAGA CTCCTCTGCT GCAAGTGACT GGTTGGCTTC AGTAGAGTCA 
TTGCTTCCTA TTACTACTGT GATCCCTGTG CCTGCCTTTC TTCTGCTGGC TCACCTCCTT 
GTTGAAGACA AAGGACAAAA TCTTCACCAA ATACTCAAGG TCACTACAGA ATTAGCCCAA 
GCAGATTCCT CCCAGGTGCC AAATCTGATT CCAGTTTTGA TGTTCAAATT GGGAAGACCA 
CTGGAACCTA TATTATATAA TGATATATTG TATACTTTAC CTAAGCTTGG TGTTCACAAG 
GTGTGTATAG GACAAATTCT ACGAGTAATA CAACTGCTTG GAACCACACC ACGACTAAGA 
GCTGTCACTT TGCGCTTGCT GACATCTTTG TGGGAAAAGC AGGACCGAGT CTATCCTGAA 
CTGCAGCGTT TCATGGCTGT GTCTGATGTA CCTTCTCTTT CGGTGGGCAA GGAAGTCCAA 
TGGGAGAAAC TGATTGCAAA AGCAGCATCA ATCAGAGATA TATGTAAGCA GAGGCCATAT 
CAACATGGTG CAGATATGCT GGCAGCTATT TCTCAGGTGT TGAATGAATG CACCAAGCCT 
GATCAAGCTA CTCCAGCAGC CTTGGTATTA CAAGGTCTTC ATGCACTCTG TCAAGCTGAG 
GTTGTTTGCA TTCGCTCCAC TTGGAATGCT CTCTCTCCAA AGCTGAGTTG TGACACAAGA 
CCTCTCATTC TGAAGACACT GAGTGAACTA TTTTCTCTAG TTCCTTCCTT AACAGTCAAT 
ACAACTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAC TCATACTCAA 
AACAAGGACC CAATTGTGGC AAATGCTGCA TATAGATCCC TGGCCAATTT TAGTGCAGGA 
GAACACACCA TTCTTCATCT GCCTGAAAAG ATAAGACCAG AAATTCCCAT TCCTGAAGAG 
TTAGATGATG ATGAAGATGA TGAGGATGTG GATCTTTCAG TTCCTGGCTC TTGCTATCTC 
AAACTGTTGT CACTCACTCC CCCTTTGGTT TTACCAGCTT TGGAGGAATT TTTTACATCA 
CTTGTGAAGC AAGAAATGGT GAATATGCCT CGTGGGATAT ATCACTCTGC ATTAAAAGGA 
GGTGTCCGCT CAGACCAAGG AAAGACTGTA GCAGGAATCC CCAATTTTAT ATTGAAAATG 
TATGAAACAA ACAAGCAACC AGGATTGAAA CCTGGCCTTG CAGGTGGTAT GTTATTTTGC 
TATGATGTTT CCATGTATCA GAGTAAAGAT GGAAAACCAT TGAATAGACT GATGGCCAGC 
AGAGGGCGAA GTTTCAAGCA GACATCACTT GCTCTTGTAC ATGAGGTTCA TATCCAGCTT 
TCAGAGTGGC ACCGTGCGAT TTTTCTTCCA CAGGCCTGGC TTGCATACAT GAATCGAGCT 
TATCATGCCA TTTTACAGGG AAGACTAGGA GAGCTGGAGT TGCAGTTAAA ACATGGAAAA 
GAAGAACCTG AGGAGGTGCA GTACAAAAAA AGCACAGCTT GGCTCTGGGT TAGAGACATG 
CTGACTGATG AGATCACCAA GGCAGCTGCA AAGGAGAGTC CGGTAGTGAA AGGCAATGCG 
CTGTTAGCTC TAAGCAGCCT TGCTGTCGTC GTATCTAGAC ATGAAGCCAG CGTCTCCTCA 
GACTCTGACG GGCTCCTGGA GGTTCAACCT AATTTCCTTT CAATGAAAGA GTGGGTTTCC 
ATGATACTTG ATACACTCTT GGTCATTGTG GATAGCCATT ACCAACCCAG AGGGCAACTT 
CTCTCCTGGT TTTATTATAA GTCCTATTCT GGTGAAAACA CAGCTAGTGC CATTGCCCGT 
TCTGCTGCCG CCACGGCTTT GTCTCTCCTT GTGCCAGTTT TCATTATCTC TTGCAAAGAG 
AAGGTTGAGG AAATCCTGAA CATGCTGACT GCCAGGTTAC CTGGGAAACC AAGTGCTGAT 
GAGTCTCAAG CCGTGCAAAT CCACATGGGC CTTGCTTTAG GGATGTTTCT CTCTCGCTTG 
TGTGAAGAGA AACTCAGTGA TATATGTGGC CAAGAGATGA ACCTTCTTCT GATGAAGTCG 
TTGGATGCCC TGGAAAATTG CTGCTTTGAC ACTAGTCTTG AATACAACAC GGGCTGTATA 
TTGGGAGTTG GACTTGTTCT GTCCCTCATG AGTCACAGCA GCCAAATGCA GTCCTGCGTT 
CACGTGACAG CATTGCTTCG GAAGCTGTCT GCATACCTAG ATGACAGTGG GAGCCAGAGC 
AGAACGTTTC AGGAGGTCCT TGCCTACACA CTTAGCTGTG TATGTACATC AGCGTTCAGT 
GCTGGAATTA TTGAGGCTAC AGAGGCTGAG GATGTTATGA ACAAGCTTCG ACTGTTAGTG 
GAGAATAGCC AGCAGACTTC AGGTTTTGCC CTGGCTTTAG GAAACGTAGT TCATGGATTG 
TCTGTGTGTG GACATGGAAA AGCTGAAGAC TTGGGCAGCA AACTACTCCC TGCCTGGATC 
AGAATTGTTC TAACAGAGGG CACTCCCACA ATGCTTTGTC TGGCAGCTCT TCATGGCATG 
GTGGCCCTGG TAGGCTCTGA AGGGGATGTA ATGCAGGTCA TTAGTGTCTC TGGGGTGATT 
GGTCTCCAGT CAAATGCAGT CTGGCTTCTT GGACATCTTC ATCTATCTAC TCTATCCTCA 
AGTCAAAGTA GAGCCTCTGT TCCTACTGAC TATAGCTATT TGCCTGAAAG CAGTTTTATT 
GGAGCAGCTA TTGGCTTCTT CATTACAGGA GGAAAAAAAG GTCCTGAATC TGTGCCTCCT 
TCCCTTCTTA AAGTAGTGAT GAAACCCATA GCAACTGTTG GAGAAAGCTA CCAATATCCT 
CCTGTGAACT GGGCTGCACT TCTCTCTCCA CTTATGAGGC TAAATTTTGG TGAAGAGATC 
CAGCAACTGT GCCTTGAAAT TATGGTGACC CAGGCACAGT CATCCCAGAA TGCAGCTGCA 
CTATTGGGCT TGTGGGTGAC ACCACCACTG ATCCACAGTC TGAGTCTGAA TACCAAGAGA 
TATCTCCTGA TATCTGCACC TCTGTGGATA AAACACATCT CTGATGAACA GATCCTGGGT 
TTTGTTGAAA ATTTAATGGT GGCAGTTTTT AAAGCAGCTT CCCCACTTGG AAATCCTGAG 
CTATGCCCGA GTGCTTTACA GGGTCTGAGC CAGGCCATGA AACTGCCCAG CCCTGCTCAC 
CACCTCTGGA GTCTGCTCTC TGAAGCTACT GGGAAAATTT TTGACCTCCT GCCAAATAAG 
ATTCGGAGAA AGGATCTAGA GCTGTATATC AGCATAGCAA AATGCCTCTT AGAAATGACA 
GATGATGATG CCAATCGGAT CGCCCAGGTT ACTAAGAGCA ACATAGAAAA GGCTGCCTTT 
GTCAAACTGT ACTTAGTCTC TCAAGGACGA TTCCCCTTGG TGAACCTGAC CGATATGCTG 
AGTGTTGCTG TGCAGCACCG TGAGAAAGAG GTGTTGGCCT GGATGATTCT GCACAGCTTA 
TACCAGGCAC GGATTGTGAG CCATGCCAAC ACGGGCGTTT TGAAGAGAAT GGAGTGGCTC 
TTGGAACTGA TGGGTTATAT TAGAAATGTT GCGTACCAGT CAACATCCTT TCAGAATACG 
GCTCTTGATG AGGCTTTGGA CTTCTTCTTG CTGATATTTG CAACCGCAGT GGTTGCATGG 
GCCGACCACA CTGCCCCTCT CCTCCTTGGT CTCAGTGCCA GTTGGTTGCC ATGGCATCAG 
GAGAATGGCC CGGCTGGGCC AGTACCAAGC TTCCTTGGCA GGAGTCCAAT GCACAGGGTC 
ACTGTGCAGG AGGTTCTCAC TCTCCTTCCC AATAGCATGG CTCTGCTGCT GCAGAAAGAG 
CCATGGAAGG AAGAGACCCA GAAGTTCATT GACTGGCTAT TCAGCATCAT GGAAAGCCCT 
AAAGAAGCCC TCTCAGCAAA GTCCAGGGAT CTTTTGAAAG CCACCCTGCT GTCCTTGAGA 
GTTCTCCCAG AGTTTAAGAA GAAAGCTGTA TGGACCAGAG CGTATGGTTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024108103.1
CDS	97..5430
Translation	MSDDIRKRFEFPNSLIQSQAAGHLIAAVLKENGFSEKIYQSTNQTPALNLLWEKCCSDNVVVRTACCEGLVALVAQDHAEFSYVLNGILNLIPSTRNTHGLIKAIMKLLQMQALKEGQGGEKNIQSVYTIRNHPHPLITVLEHRPDCWPVFLQQLTAFFQQCPERSEVSCIQIMAPFLWYLYCEPSQLQEYAKLRLALLKVLLQPQVLCDKDQPSILQQQILQLCCDIVPCLQIKDLIQTTEAMMFIEEIYLSLLRHPVFWKIQLTQMTLQLLCVGEVSLKITGECSSSIHLLEHSVELLKEDFPVELVIIGIALLLLQTPASQQKPILNLALKLFSVTEDQKIPKSSLLLVMPILQILSSTALEDCISVDEEGPSRQQLALNLLEMIQQECYRDDHQKLSYKLVCPVTSMYGSVFTAWRILEVMTDSSAASDWLASVESLLPITTVIPVPAFLLLAHLLVEDKGQNLHQILKVTTELAQADSSQVPNLIPVLMFKLGRPLEPILYNDILYTLPKLGVHKVCIGQILRVIQLLGTTPRLRAVTLRLLTSLWEKQDRVYPELQRFMAVSDVPSLSVGKEVQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQATPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDTRPLILKTLSELFSLVPSLTVNTTEYENFKVQVLSFLWTHTQNKDPIVANAAYRSLANFSAGEHTILHLPEKIRPEIPIPEELDDDEDDEDVDLSVPGSCYLKLLSLTPPLVLPALEEFFTSLVKQEMVNMPRGIYHSALKGGVRSDQGKTVAGIPNFILKMYETNKQPGLKPGLAGGMLFCYDVSMYQSKDGKPLNRLMASRGRSFKQTSLALVHEVHIQLSEWHRAIFLPQAWLAYMNRAYHAILQGRLGELELQLKHGKEEPEEVQYKKSTAWLWVRDMLTDEITKAAAKESPVVKGNALLALSSLAVVVSRHEASVSSDSDGLLEVQPNFLSMKEWVSMILDTLLVIVDSHYQPRGQLLSWFYYKSYSGENTASAIARSAAATALSLLVPVFIISCKEKVEEILNMLTARLPGKPSADESQAVQIHMGLALGMFLSRLCEEKLSDICGQEMNLLLMKSLDALENCCFDTSLEYNTGCILGVGLVLSLMSHSSQMQSCVHVTALLRKLSAYLDDSGSQSRTFQEVLAYTLSCVCTSAFSAGIIEATEAEDVMNKLRLLVENSQQTSGFALALGNVVHGLSVCGHGKAEDLGSKLLPAWIRIVLTEGTPTMLCLAALHGMVALVGSEGDVMQVISVSGVIGLQSNAVWLLGHLHLSTLSSSQSRASVPTDYSYLPESSFIGAAIGFFITGGKKGPESVPPSLLKVVMKPIATVGESYQYPPVNWAALLSPLMRLNFGEEIQQLCLEIMVTQAQSSQNAAALLGLWVTPPLIHSLSLNTKRYLLISAPLWIKHISDEQILGFVENLMVAVFKAASPLGNPELCPSALQGLSQAMKLPSPAHHLWSLLSEATGKIFDLLPNKIRRKDLELYISIAKCLLEMTDDDANRIAQVTKSNIEKAAFVKLYLVSQGRFPLVNLTDMLSVAVQHREKEVLAWMILHSLYQARIVSHANTGVLKRMEWLLELMGYIRNVAYQSTSFQNTALDEALDFFLLIFATAVVAWADHTAPLLLGLSASWLPWHQENGPAGPVPSFLGRSPMHRVTVQEVLTLLPNSMALLLQKEPWKEETQKFIDWLFSIMESPKEALSAKSRDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_024252335.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii focadhesin (FOCAD), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024252335.1

ORF Insert Sequence:

ATGTCAGATG ATATCAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA ATCACAGGCT 
GCGGGTCATC TTATTGCTGC AGTCCTAAAG GAGAATGGTT TTTCAGAAAA GATTTACCAA 
TCTACAAATC AGACTCCTGC TTTGAACTTG CTGTGGGAGA AGTGTTGCAG TGACAATGTA 
GTGGTTCGAA CAGCCTGCTG TGAAGGTCTG GTGGCACTCG TTGCTCAGGA TCATGCAGAG 
TTCAGCTATG TTCTCAATGG GATACTCAAC TTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCATTATGAA ATTACTACAA ATGCAAGCTC TTAAGGAAGG ACAAGGTGGG 
GAAAAGAATA TTCAGAGTGT ATATACCATT AGAAATCATC CTCATCCTTT GATCACTGTG 
CTTGAACACA GACCTGATTG TTGGCCAGTG TTTTTGCAGC AGCTGACAGC ATTTTTCCAG 
CAGTGCCCTG AAAGGTCAGA AGTTTCATGC ATTCAAATAA TGGCACCATT TCTGTGGTAT 
CTGTATTGTG AACCATCTCA GTTACAAGAA TATGCTAAAC TCCGACTAGC CCTGCTGAAA 
GTCTTACTTC AACCCCAGGT TCTTTGTGAC AAGGATCAAC CATCAATACT GCAACAGCAG 
ATACTTCAAC TGTGTTGTGA CATAGTTCCA TGTTTGCAGA TAAAAGATTT GATACAGACA 
ACAGAAGCGA TGATGTTTAT TGAGGAAATA TATTTAAGCC TTTTGCGTCA TCCTGTTTTC 
TGGAAAATTC AGCTTACCCA GATGACTCTT CAGCTGCTGT GTGTCGGTGA AGTCAGCTTA 
AAGATAACTG GTGAATGTTC ATCTTCAATT CACCTCTTAG AGCACAGTGT TGAACTTCTG 
AAGGAGGATT TTCCTGTTGA ACTGGTCATA ATTGGAATAG CTTTACTACT TCTACAGACT 
CCAGCAAGTC AGCAGAAGCC AATCTTAAAT CTAGCTTTGA AGCTCTTCTC TGTTACTGAG 
GATCAGAAAA TCCCAAAGTC CTCTCTGCTG CTAGTGATGC CAATTCTGCA GATACTGTCT 
TCTACTGCCT TGGAAGACTG TATATCTGTG GATGAAGAAG GTCCCTCTAG GCAGCAGTTG 
GCTCTAAACC TTTTGGAAAT GATACAGCAG GAATGTTACA GAGATGACCA CCAAAAGCTC 
TCCTACAAGC TTGTGTGCCC TGTAACCAGT ATGTATGGTT CAGTATTTAC AGCCTGGAGG 
ATTCTTGAAG TAATGACAGA CTCCTCTGCT GCAAGTGACT GGTTGGCTTC AGTAGAGTCA 
TTGCTTCCTA TTACTACTGT GATCCCTGTG CCTGCCTTTC TTCTGCTGGC TCACCTCCTT 
GTTGAAGACA AAGGACAAAA TCTTCACCAA ATACTCAAGG TCACTACAGA ATTAGCCCAA 
GCAGATTCCT CCCAGGTGCC AAATCTGATT CCAGTTTTGA TGTTCAAATT GGGAAGACCA 
CTGGAACCTA TATTATATAA TGATATATTG TATACTTTAC CTAAGCTTGG TGTTCACAAG 
GTGTGTATAG GACAAATTCT ACGAGTAATA CAACTGCTTG GAACCACACC ACGACTAAGA 
GCTGTCACTT TGCGCTTGCT GACATCTTTG TGGGAAAAGC AGGACCGAGT CTATCCTGAA 
CTGCAGCGTT TCATGGCTGT GTCTGATGTA CCTTCTCTTT CGGTGGGCAA GGAAGTCCAA 
TGGGAGAAAC TGATTGCAAA AGCAGCATCA ATCAGAGATA TATGTAAGCA GAGGCCATAT 
CAACATGGTG CAGATATGCT GGCAGCTATT TCTCAGGTGT TGAATGAATG CACCAAGCCT 
GATCAAGCTA CTCCAGCAGC CTTGGTATTA CAAGGTCTTC ATGCACTCTG TCAAGCTGAG 
GTTGTTTGCA TTCGCTCCAC TTGGAATGCT CTCTCTCCAA AGCTGAGTTG TGACACAAGA 
CCTCTCATTC TGAAGACACT GAGTGAACTA TTTTCTCTAG TTCCTTCCTT AACAGTCAAT 
ACAACTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAC TCATACTCAA 
AACAAGGACC CAATTGTGGC AAATGCTGCA TATAGATCCC TGGCCAATTT TAGTGCAGGA 
GAACACACCA TTCTTCATCT GCCTGAAAAG ATAAGACCAG AAATTCCCAT TCCTGAAGAG 
TTAGATGATG ATGAAGATGA TGAGGATGTG GATCTTTCAG TTCCTGGCTC TTGCTATCTC 
AAACTGTTGT CACTCACTCC CCCTTTGGTT TTACCAGCTT TGGAGGAATT TTTTACATCA 
CTTGTGAAGC AAGAAATGGT GAATATGCCT CGTGGGATAT ATCACTCTGC ATTAAAAGGA 
GGTGTCCGCT CAGACCAAGG AAAGACTGTA GCAGGAATCC CCAATTTTAT ATTGAAAATG 
TATGAAACAA ACAAGCAACC AGGATTGAAA CCTGGCCTTG CAGGTGGTAT GTTATTTTGC 
TATGATGTTT CCATGTATCA GAGTAAAGAT GGAAAACCAT TGAATAGACT GATGGCCAGC 
AGAGGGCGAA GTTTCAAGCA GACATCACTT GCTCTTGTAC ATGAGGTTCA TATCCAGCTT 
TCAGAGTGGC ACCGTGCGAT TTTTCTTCCA CAGGCCTGGC TTGCATACAT GAATCGAGCT 
TATCATGCCA TTTTACAGGG AAGACTAGGA GAGCTGGAGT TGCAGTTAAA ACATGGAAAA 
GAAGAACCTG AGGAGGTGCA GTACAAAAAA AGCACAGCTT GGCTCTGGGT TAGAGACATG 
CTGACTGATG AGATCACCAA GGCAGCTGCA AAGGAGAGTC CGGTAGTGAA AGGCAATGCG 
CTGTTAGCTC TAAGCAGCCT TGCTGTCGTC GTATCTAGAC ATGAAGCCAG CGTCTCCTCA 
GACTCTGACG GGCTCCTGGA GGTTCAACCT AATTTCCTTT CAATGAAAGA GTGGGTTTCC 
ATGATACTTG ATACACTCTT GGTCATTGTG GATAGCCATT ACCAACCCAG AGGGCAACTT 
CTCTCCTGGT TTTATTATAA GTCCTATTCT GGTGAAAACA CAGCTAGTGC CATTGCCCGT 
TCTGCTGCCG CCACGGCTTT GTCTCTCCTT GTGCCAGTTT TCATTATCTC TTGCAAAGAG 
AAGGTTGAGG AAATCCTGAA CATGCTGACT GCCAGGTTAC CTGGGAAACC AAGTGCTGAT 
GAGTCTCAAG CCGTGCAAAT CCACATGGGC CTTGCTTTAG GGATGTTTCT CTCTCGCTTG 
TGTGAAGAGA AACTCAGTGA TATATGTGGC CAAGAGATGA ACCTTCTTCT GATGAAGTCG 
TTGGATGCCC TGGAAAATTG CTGCTTTGAC ACTAGTCTTG AATACAACAC GGGCTGTATA 
TTGGGAGTTG GACTTGTTCT GTCCCTCATG AGTCACAGCA GCCAAATGCA GTCCTGCGTT 
CACGTGACAG CATTGCTTCG GAAGCTGTCT GCATACCTAG ATGACAGTGG GAGCCAGAGC 
AGAACGTTTC AGGAGGTCCT TGCCTACACA CTTAGCTGTG TATGTACATC AGCGTTCAGT 
GCTGGAATTA TTGAGGCTAC AGAGGCTGAG GATGTTATGA ACAAGCTTCG ACTGTTAGTG 
GAGAATAGCC AGCAGACTTC AGGTTTTGCC CTGGCTTTAG GAAACGTAGT TCATGGATTG 
TCTGTGTGTG GACATGGAAA AGCTGAAGAC TTGGGCAGCA AACTACTCCC TGCCTGGATC 
AGAATTGTTC TAACAGAGGG CACTCCCACA ATGCTTTGTC TGGCAGCTCT TCATGGCATG 
GTGGCCCTGG TAGGCTCTGA AGGGGATGTA ATGCAGGTCA TTAGTGTCTC TGGGGTGATT 
GGTCTCCAGT CAAATGCAGT CTGGCTTCTT GGACATCTTC ATCTATCTAC TCTATCCTCA 
AGTCAAAGTA GAGCCTCTGT TCCTACTGAC TATAGCTATT TGCCTGAAAG CAGTTTTATT 
GGAGCAGCTA TTGGCTTCTT CATTACAGGA GGAAAAAAAG GTCCTGAATC TGTGCCTCCT 
TCCCTTCTTA AAGTAGTGAT GAAACCCATA GCAACTGTTG GAGAAAGCTA CCAATATCCT 
CCTGTGAACT GGGCTGCACT TCTCTCTCCA CTTATGAGGC TAAATTTTGG TGAAGAGATC 
CAGCAACTGT GCCTTGAAAT TATGGTGACC CAGGCACAGT CATCCCAGAA TGCAGCTGCA 
CTATTGGGCT TGTGGGTGAC ACCACCACTG ATCCACAGTC TGAGTCTGAA TACCAAGAGA 
TATCTCCTGA TATCTGCACC TCTGTGGATA AAACACATCT CTGATGAACA GATCCTGGGT 
TTTGTTGAAA ATTTAATGGT GGCAGTTTTT AAAGCAGCTT CCCCACTTGG AAATCCTGAG 
CTATGCCCGA GTGCTTTACA GGGTCTGAGC CAGGCCATGA AACTGCCCAG CCCTGCTCAC 
CACCTCTGGA GTCTGCTCTC TGAAGCTACT GGGAAAATTT TTGACCTCCT GCCAAATAAG 
ATTCGGAGAA AGGATCTAGA GCTGTATATC AGCATAGCAA AATGCCTCTT AGAAATGACA 
GATGATGATG CCAATCGGAT CGCCCAGGTT ACTAAGAGCA ACATAGAAAA GGCTGCCTTT 
GTCAAACTGT ACTTAGTCTC TCAAGGACGA TTCCCCTTGG TGAACCTGAC CGATATGCTG 
AGTGTTGCTG TGCAGCACCG TGAGAAAGAG GTGTTGGCCT GGATGATTCT GCACAGCTTA 
TACCAGGCAC GGATTGTGAG CCATGCCAAC ACGGGCGTTT TGAAGAGAAT GGAGTGGCTC 
TTGGAACTGA TGGGTTATAT TAGAAATGTT GCGTACCAGT CAACATCCTT TCAGAATACG 
GCTCTTGATG AGGCTTTGGA CTTCTTCTTG CTGATATTTG CAACCGCAGT GGTTGCATGG 
GCCGACCACA CTGCCCCTCT CCTCCTTGGT CTCAGTGCCA GTTGGTTGCC ATGGCATCAG 
GAGAATGGCC CGGCTGGGCC AGTACCAAGC TTCCTTGGCA GGAGTCCAAT GCACAGGGTC 
ACTGTGCAGG AGGTTCTCAC TCTCCTTCCC AATAGCATGG CTCTGCTGCT GCAGAAAGAG 
CCATGGAAGG AAGAGACCCA GAAGTTCATT GACTGGCTAT TCAGCATCAT GGAAAGCCCT 
AAAGAAGCCC TCTCAGCAAA GTCCAGGGAT CTTTTGAAAG CCACCCTGCT GTCCTTGAGA 
GTTCTCCCAG AGTTTAAGAA GAAAGCTGTA TGGACCAGAG CGTATGGTTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FOCAD ( XM_009244393.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009242668.1 Pongo abelii focadhesin (FOCAD), transcript variant X5, mRNA.

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CloneID	OSa07887
Clone ID Related Accession (Same CDS sequence)	XM_009244393.1 , XM_009244395.1 , XM_009244392.1 , XM_009244394.1
Accession Version	XM_009244393.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-22
Organism	Pongo abelii(Sumatran orangutan)
Product	focadhesin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002883424.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pongo abelii Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGATG ATATCAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA ATCACAGGCT 
GCGGGTCATC TTATTGCTGC AGTCCTAAAG GAGAATGGTT TTTCAGAAAA GATTTACCAA 
TCTACAAATC AGACTCCTGC TTTGAACTTG CTGTGGGAGA AGTGTTGCAG TGACAATGTA 
GTGGTTCGAA CAGCCTGCTG TGAAGGTCTG GTGGCACTCG TTGCTCAGGA TCATGCAGAG 
TTCAGCTATG TTCTCAATGG GATACTCAAC TTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCATTATGAA ATTACTACAA ATGCAAGCTC TTAAGGAAGG ACAAGGTGGG 
GAAAAGAATA TTCAGAGGGT ATATACCATT AGAAATCATC CTCATCCTTT GATCACTGTG 
CTTGAACACA GACCTGATTG TTGGCCAGTG TTTTTGCAGC AGCTGACAGC ATTTTTCCAG 
CAGTGCCCTG AAAGGTCAGA AGTTTCATGC ATTCAAATAA TGGCACCATT TCTGTGGTAT 
CTGTATTGTG AACCATCTCA GTTACAAGAA TATGCTAAAC TCCGACTAGC CCTGCTGAAA 
GTCTTACTTC AACCCCAGGT TCTTTGTGAC AAGGATCAAC CATCAATACT GCAACAGCAG 
ATACTTCAAC TGTGTTGTGA CATAGTTCCA TGTTTGCAGA TAAAAGATTT GATACAGACA 
ACAGAAGCGA TGATGTTTAT TGAGGAAGTA TATTTAAGCC TTTTGCGTCA TCCTGTTTTC 
TGGAAAATTC AGCTTACCCA GATGACTCTT CAGCTGCTGT GTGTCGGTGA AGTCAGCTTA 
AAGATAACTG GTGAATGTTC ATCTTCAATT CACCTCTTAG AGCACAGTGT TGAACTTCTG 
AAGGAGGATT TTCCTGTTGA ACTGGTCATA ATTGGAATAG CTTTACTACT TCTACAGACT 
CCAGCAAGTC AGCAGAAGCC AATCTTAAAT CTAGCTTTGA AGCTCTTCTC TGTTACTGAG 
GATCAGAAAA TCCCAAAGTC CTCTCTGCTG CTAGTGATGC CAATTCTGCA GATACTGTCT 
TCTACTGCCT TGGAAGACTG TATATCTGTG GATGAAGAAG GTCCCTCTAG GCAGCAGTTG 
GCTCTAAACC TTTTGGAAAT GATACAGCAG GAATGTTACA GAGATGACCA CCAAAAGCTC 
TCCTACAAGC TTGTGTGCCC TGTAACCAGT ATGTATGGTT CAGTATTTAC AGCCTGGAGG 
ATTCTTGAAG TAATGACAGA CTCCTCTGCT GCAAGTGACT GGTTGGCTTC AGTAGAGTCA 
TTGCTTCCTA TTACTACTGT GATCCCTGTG CCTGCCTTTC TTCTGCTGGC TCACCTCCTT 
GTTGAAGACA AAGGACAAAA TCTTCACCAA ATACTCAAGG TCACTACAGA ATTAGCCCAA 
GCAGATTCCT CCCAGGTGCC AAATCTGATT CCAGTTTTGA TGTTCAAATT GGGAAGACCA 
CTGGAACCTA TATTATATAA TGATATATTG TATACTTTAC CTAAGCTTGG TGTTCACAAG 
GTGTGTATAG GACAAATTCT ACGAGTAATA CAACTGCTTG GAACCACACC ACGACTAAGA 
GCTGTCACTT TGCGCTTGCT GACATCTTTG TGGGAAAAGC AGGACCGAGT CTATCCTGAA 
CTGCAGCGTT TCATGGCTGT GTCTGATGTA CCTTCTCTTT CGGTGGGCAA GGAAGTCCAA 
TGGGAGAAAC TGATTGCAAA AGCAGCATCA ATCAGAGATA TATGTAAGCA GAGGCCATAT 
CAACATGGTG CAGATATGCT GGCAGCTATT TCTCAGGTGT TGAATGAATG CACCAAGCCT 
GATCAAGCTA CTCCAGCAGC CTTGGTATTA CAAGGTCTTC ATGCACTCTG TCAAGCTGAG 
GTTGTTTGCA TTCGCTCCAC TTGGAATGCT CTCTCTCCAA AGCTGAGTTG TGACACAAGA 
CCTCTCATTC TGAAGACACT GAGTGAACTA TTTTCTCTAG TTCCTTCCTT AACAGTCAAT 
ACAACTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAC TCATACTCAA 
AACAAGGACC CAATTGTGGC AAATGCTGCA TATAGATCCC TGGCCAATTT TAGTGCAGGA 
GAACACACCA TTCTTCATCT GCCTGAAAAG ATAAGACCAG AAATTCCCAT TCCTGAAGAG 
TTAGATGATG ATGAAGATGA TGAGGATGTG GATCTTTCAG TTCCTGGCTC TTGCTATCTC 
AAACTGTTGT CACTCACTCC CCCTTTGGTT TTACCAGCTT TGGAGGAATT TTTTACATCA 
CTTGTGAAGC AAGAAATGGT GAATATGCCT CGTGGGATAT ATCACTCTGC ATTAAAAGGA 
GGTGTCCGCT CAGACCAAGG AAAGACTGTA GCAGGAATCC CCAATTTTAT ATTGAAAATG 
TATGAAACAA ACAAGCAACC AGGATTGAAA CCTGGCCTTG CAGGTGGTAT GTTATTTTGC 
TATGATGTTT CCATGTATCA GAGTAAAGAT GGAAAACCAT TGAATAGACT GATGGCCAGC 
AGAGGGCGAA GTTTCAAGCA GACATCACTT GCTCTTGTAC ATGAGGTTCA TATCCAGCTT 
TCAGAGTGGC ACCGTGCGAT TTTTCTTCCA CAGGCCTGGC TTGCATACAT GAATCGAGCT 
TATCATGCCA TTTTACAGGG AAGACTAGGA GAGCTGGAGT TGCAGTTAAA ACATGGAAAA 
GAAGAACCTG AGGAGGTGCA GTACAAAAAA AGCACAGCTT GGCTCTGGGT TAGAGACATG 
CTGACTGATG AGATCACCAA GGCAGCTGCA AAGGAGAGTC CGGTAGTGAA AGGCAATGCG 
CTGTTAGCTC TAAGCAGCCT TGCTGTCGTC GTATCTAGAC ATGAAGCCAG CCTCTCCTCA 
GACTCTGACG GGCTCCTGGA GGTTCAACCT AATTTCCTTT CAATGAAAGA GTGGGTTTCC 
ATGATACTTG ATACACTCTT GGTCATTGTG GATAGCCATT ACCAACCCAG AGGGCAACTT 
CTCTCCTGGT TTTATTATAA GTCCTATTCT GGTGAAAACA CAGCTAGTGC CATTGCCCGT 
TCTGCTGCCG CCACGGCTTT GTCTCTCCTT GTGCCAGTTT TCATTATCTC TTGCAAAGAG 
AAGGTTGAGG AAATCCTGAA CATGCTGACT GCCAGGTTAC CTGGGAAACC AAGTGCTGAT 
GAGTCTCAAG CCGTGCAAAT CCACATGGGC CTTGCTTTAG GGATGTTTCT CTCTCGCTTG 
TGTGAAGAGA AACTCAGTGA TATATGTGGC CAAGAGATGA ACCTTCTTCT GATGAAGTCG 
TTGGATGCCC TGGAAAATTG CTGCTTTGAC ACTAGTCTTG AATACAACAC GGGCTGTATA 
TTGGGAGTTG GACTTGTTCT GTCCCTCATG AGTCACAGCA GCCAAATGCA GTCCTGCGTT 
CACGTGACAG CATTGCTTCG GAAGCTGTCT GCATACCTAG ATGACAGTGG GAGCCAGAGC 
AGAACGTTTC AGGAGGTCCT TGCCTACACA CTTAGCTGTG TATGTACATC AGCGTTCAGT 
GCTGGAATTA TTGAGGCTAC AGAGGCTGAG GATGTTATGA ACAAGCTTCG ACTGTTAGTG 
GAGAATAGCC AGCAGACTTC AGGTTTTGCC CTGGCTTTAG GAAACGTAGT TCATGGATTG 
TCTGTGTGTG GACATGGAAA AGCTGAAGAC TTGGGCAGCA AACTACTCCC TGCCTGGATC 
AGAATTGTTC TAACAGAGGG CACTCCCACA ATGCTTTGTC TGGCAGCTCT TCATGGCATG 
GTGGCCCTGG TAGGCTCTGA AGGGGATGTA ATGCAGCTGA AATCAGAAGC CATCCAGACC 
TCTCATTTTC AAGGCAGACT TAATGAAGTC ATTAGAACCT TAACTCAGGT CATTAGTGTC 
TCTGGGGTGA TTGGTCTCCA GTCAAATGCA GTCTGGCTTC TTGGACATCT TCATCTATCT 
ACTCTATCCT CAAGTCAAAG TAGAGCCTCT GTTCCTACTG ACTATAGCTA TTTGCCTGAA 
AGCAGTTTTA TTGGAGCAGC TATTGGCTTC TTCATTACAG GAGGAAAAAA AGGTCCTGAA 
TCTGTGCCTC CTTCCCTTCT TAAAGTAGTG ATGAAACCCA TAGCAACTGT TGGAGAAAGC 
TACCAATATC CTCCTGTGAA CTGGGCTGCA CTTCTCTCTC CACTTATGAG GCTAAATTTT 
GGTGAAGAGA TCCAGCAACT GTGCCTTGAA ATTATGGTGA CCCAGGCACA GTCATCCCAG 
AATGCAGCTG CACTATTGGG CTTGTGGGTG ACACCACCAC TGATCCACAG TCTGAGTCTG 
AATACCAAGA GATATCTCCT GATATCTGCA CCTCTGTGGA TAAAACACAT CTCTGATGAA 
CAGATCCTGG GTTTTGTTGA AAATTTAATG GTGGCAGTTT TTAAAGCAGC TTCCCCACTT 
GGAAATCCTG AGCTATGCCC GAGTGCTTTA CAGGGTCTGA GCCAGGCCAT GAAACTGCCC 
AGCCCTGCTC ACCACCTCTG GAGTCTGCTC TCTGAAGCTA CTGGGAAAAT TTTTGACCTC 
CTGCCAAATA AGATTCGGAG AAAGGATCTA GAGCTGTATA TCAGCATAGC AAAATGCCTC 
TTAGAAATGA CAGATGATGA TGCCAATCGG ATCGCCCAGG TTACTAAGAG CAACATAGAA 
AAGGCTGCCT TTGTCAAACT GTACTTAGTC TCTCAAGGAC GATTCCCCTT GGTGAACCTG 
ACCGATATGC TGAGTGTTGC TGTGCAGCAC CGTGAGAAAG AGGTGTTGGC CTGGATGATT 
CTGCACAGCT TATACCAGGC ACGGATTGTG AGCCATGCCA ACACGGGCGT TTTGAAGAGA 
ATGGAGTGGC TCTTGGAACT GATGGGTTAT ATTAGAAATG TTGCGTACCA GTCAACATCC 
TTTCAGAATA CGGCTCTTGA TGAGGCTTTG GACTTCTTCT TGCTGATATT TGCAACCGCA 
GTGGTTGCAT GGGCCGACCA CACTGCCCCT CTCCTCCTTG GTCTCAGTGC CAGTTGGTTG 
CCATGGCATC AGGAGAATGG CCCGGCTGGG CCAGTACCAA GCTTCCTTGG CAGGAGTCCA 
ATGCACAGGG TCACTGTGCA GGAGGTTCTC ACTCTCCTTC CCAATAGCAT GGCTCTGCTG 
CTGCAGAAAG AGCCATGGAA GGAAGAGACC CAGAAGTTCA TTGACTGGCT ATTCAGCATC 
ATGGAAAGCC CTAAAGAAGC CCTCTCAGCA AAGTCCAGGG ATCTTTTGAA AGCCACCCTG 
CTGTCCTTGA GAGTTCTCCC AGAGTTTAAG AAGAAAGCTG TATGGACCAG AGCGTATGGT 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009242668.1
CDS	52..5457
Translation	MSDDIRKRFEFPNSLIQSQAAGHLIAAVLKENGFSEKIYQSTNQTPALNLLWEKCCSDNVVVRTACCEGLVALVAQDHAEFSYVLNGILNLIPSTRNTHGLIKAIMKLLQMQALKEGQGGEKNIQRVYTIRNHPHPLITVLEHRPDCWPVFLQQLTAFFQQCPERSEVSCIQIMAPFLWYLYCEPSQLQEYAKLRLALLKVLLQPQVLCDKDQPSILQQQILQLCCDIVPCLQIKDLIQTTEAMMFIEEVYLSLLRHPVFWKIQLTQMTLQLLCVGEVSLKITGECSSSIHLLEHSVELLKEDFPVELVIIGIALLLLQTPASQQKPILNLALKLFSVTEDQKIPKSSLLLVMPILQILSSTALEDCISVDEEGPSRQQLALNLLEMIQQECYRDDHQKLSYKLVCPVTSMYGSVFTAWRILEVMTDSSAASDWLASVESLLPITTVIPVPAFLLLAHLLVEDKGQNLHQILKVTTELAQADSSQVPNLIPVLMFKLGRPLEPILYNDILYTLPKLGVHKVCIGQILRVIQLLGTTPRLRAVTLRLLTSLWEKQDRVYPELQRFMAVSDVPSLSVGKEVQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQATPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDTRPLILKTLSELFSLVPSLTVNTTEYENFKVQVLSFLWTHTQNKDPIVANAAYRSLANFSAGEHTILHLPEKIRPEIPIPEELDDDEDDEDVDLSVPGSCYLKLLSLTPPLVLPALEEFFTSLVKQEMVNMPRGIYHSALKGGVRSDQGKTVAGIPNFILKMYETNKQPGLKPGLAGGMLFCYDVSMYQSKDGKPLNRLMASRGRSFKQTSLALVHEVHIQLSEWHRAIFLPQAWLAYMNRAYHAILQGRLGELELQLKHGKEEPEEVQYKKSTAWLWVRDMLTDEITKAAAKESPVVKGNALLALSSLAVVVSRHEASLSSDSDGLLEVQPNFLSMKEWVSMILDTLLVIVDSHYQPRGQLLSWFYYKSYSGENTASAIARSAAATALSLLVPVFIISCKEKVEEILNMLTARLPGKPSADESQAVQIHMGLALGMFLSRLCEEKLSDICGQEMNLLLMKSLDALENCCFDTSLEYNTGCILGVGLVLSLMSHSSQMQSCVHVTALLRKLSAYLDDSGSQSRTFQEVLAYTLSCVCTSAFSAGIIEATEAEDVMNKLRLLVENSQQTSGFALALGNVVHGLSVCGHGKAEDLGSKLLPAWIRIVLTEGTPTMLCLAALHGMVALVGSEGDVMQLKSEAIQTSHFQGRLNEVIRTLTQVISVSGVIGLQSNAVWLLGHLHLSTLSSSQSRASVPTDYSYLPESSFIGAAIGFFITGGKKGPESVPPSLLKVVMKPIATVGESYQYPPVNWAALLSPLMRLNFGEEIQQLCLEIMVTQAQSSQNAAALLGLWVTPPLIHSLSLNTKRYLLISAPLWIKHISDEQILGFVENLMVAVFKAASPLGNPELCPSALQGLSQAMKLPSPAHHLWSLLSEATGKIFDLLPNKIRRKDLELYISIAKCLLEMTDDDANRIAQVTKSNIEKAAFVKLYLVSQGRFPLVNLTDMLSVAVQHREKEVLAWMILHSLYQARIVSHANTGVLKRMEWLLELMGYIRNVAYQSTSFQNTALDEALDFFLLIFATAVVAWADHTAPLLLGLSASWLPWHQENGPAGPVPSFLGRSPMHRVTVQEVLTLLPNSMALLLQKEPWKEETQKFIDWLFSIMESPKEALSAKSRDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_009244393.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii focadhesin (FOCAD), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009244393.1

ORF Insert Sequence:

ATGTCAGATG ATATCAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA ATCACAGGCT 
GCGGGTCATC TTATTGCTGC AGTCCTAAAG GAGAATGGTT TTTCAGAAAA GATTTACCAA 
TCTACAAATC AGACTCCTGC TTTGAACTTG CTGTGGGAGA AGTGTTGCAG TGACAATGTA 
GTGGTTCGAA CAGCCTGCTG TGAAGGTCTG GTGGCACTCG TTGCTCAGGA TCATGCAGAG 
TTCAGCTATG TTCTCAATGG GATACTCAAC TTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCATTATGAA ATTACTACAA ATGCAAGCTC TTAAGGAAGG ACAAGGTGGG 
GAAAAGAATA TTCAGAGGGT ATATACCATT AGAAATCATC CTCATCCTTT GATCACTGTG 
CTTGAACACA GACCTGATTG TTGGCCAGTG TTTTTGCAGC AGCTGACAGC ATTTTTCCAG 
CAGTGCCCTG AAAGGTCAGA AGTTTCATGC ATTCAAATAA TGGCACCATT TCTGTGGTAT 
CTGTATTGTG AACCATCTCA GTTACAAGAA TATGCTAAAC TCCGACTAGC CCTGCTGAAA 
GTCTTACTTC AACCCCAGGT TCTTTGTGAC AAGGATCAAC CATCAATACT GCAACAGCAG 
ATACTTCAAC TGTGTTGTGA CATAGTTCCA TGTTTGCAGA TAAAAGATTT GATACAGACA 
ACAGAAGCGA TGATGTTTAT TGAGGAAGTA TATTTAAGCC TTTTGCGTCA TCCTGTTTTC 
TGGAAAATTC AGCTTACCCA GATGACTCTT CAGCTGCTGT GTGTCGGTGA AGTCAGCTTA 
AAGATAACTG GTGAATGTTC ATCTTCAATT CACCTCTTAG AGCACAGTGT TGAACTTCTG 
AAGGAGGATT TTCCTGTTGA ACTGGTCATA ATTGGAATAG CTTTACTACT TCTACAGACT 
CCAGCAAGTC AGCAGAAGCC AATCTTAAAT CTAGCTTTGA AGCTCTTCTC TGTTACTGAG 
GATCAGAAAA TCCCAAAGTC CTCTCTGCTG CTAGTGATGC CAATTCTGCA GATACTGTCT 
TCTACTGCCT TGGAAGACTG TATATCTGTG GATGAAGAAG GTCCCTCTAG GCAGCAGTTG 
GCTCTAAACC TTTTGGAAAT GATACAGCAG GAATGTTACA GAGATGACCA CCAAAAGCTC 
TCCTACAAGC TTGTGTGCCC TGTAACCAGT ATGTATGGTT CAGTATTTAC AGCCTGGAGG 
ATTCTTGAAG TAATGACAGA CTCCTCTGCT GCAAGTGACT GGTTGGCTTC AGTAGAGTCA 
TTGCTTCCTA TTACTACTGT GATCCCTGTG CCTGCCTTTC TTCTGCTGGC TCACCTCCTT 
GTTGAAGACA AAGGACAAAA TCTTCACCAA ATACTCAAGG TCACTACAGA ATTAGCCCAA 
GCAGATTCCT CCCAGGTGCC AAATCTGATT CCAGTTTTGA TGTTCAAATT GGGAAGACCA 
CTGGAACCTA TATTATATAA TGATATATTG TATACTTTAC CTAAGCTTGG TGTTCACAAG 
GTGTGTATAG GACAAATTCT ACGAGTAATA CAACTGCTTG GAACCACACC ACGACTAAGA 
GCTGTCACTT TGCGCTTGCT GACATCTTTG TGGGAAAAGC AGGACCGAGT CTATCCTGAA 
CTGCAGCGTT TCATGGCTGT GTCTGATGTA CCTTCTCTTT CGGTGGGCAA GGAAGTCCAA 
TGGGAGAAAC TGATTGCAAA AGCAGCATCA ATCAGAGATA TATGTAAGCA GAGGCCATAT 
CAACATGGTG CAGATATGCT GGCAGCTATT TCTCAGGTGT TGAATGAATG CACCAAGCCT 
GATCAAGCTA CTCCAGCAGC CTTGGTATTA CAAGGTCTTC ATGCACTCTG TCAAGCTGAG 
GTTGTTTGCA TTCGCTCCAC TTGGAATGCT CTCTCTCCAA AGCTGAGTTG TGACACAAGA 
CCTCTCATTC TGAAGACACT GAGTGAACTA TTTTCTCTAG TTCCTTCCTT AACAGTCAAT 
ACAACTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAC TCATACTCAA 
AACAAGGACC CAATTGTGGC AAATGCTGCA TATAGATCCC TGGCCAATTT TAGTGCAGGA 
GAACACACCA TTCTTCATCT GCCTGAAAAG ATAAGACCAG AAATTCCCAT TCCTGAAGAG 
TTAGATGATG ATGAAGATGA TGAGGATGTG GATCTTTCAG TTCCTGGCTC TTGCTATCTC 
AAACTGTTGT CACTCACTCC CCCTTTGGTT TTACCAGCTT TGGAGGAATT TTTTACATCA 
CTTGTGAAGC AAGAAATGGT GAATATGCCT CGTGGGATAT ATCACTCTGC ATTAAAAGGA 
GGTGTCCGCT CAGACCAAGG AAAGACTGTA GCAGGAATCC CCAATTTTAT ATTGAAAATG 
TATGAAACAA ACAAGCAACC AGGATTGAAA CCTGGCCTTG CAGGTGGTAT GTTATTTTGC 
TATGATGTTT CCATGTATCA GAGTAAAGAT GGAAAACCAT TGAATAGACT GATGGCCAGC 
AGAGGGCGAA GTTTCAAGCA GACATCACTT GCTCTTGTAC ATGAGGTTCA TATCCAGCTT 
TCAGAGTGGC ACCGTGCGAT TTTTCTTCCA CAGGCCTGGC TTGCATACAT GAATCGAGCT 
TATCATGCCA TTTTACAGGG AAGACTAGGA GAGCTGGAGT TGCAGTTAAA ACATGGAAAA 
GAAGAACCTG AGGAGGTGCA GTACAAAAAA AGCACAGCTT GGCTCTGGGT TAGAGACATG 
CTGACTGATG AGATCACCAA GGCAGCTGCA AAGGAGAGTC CGGTAGTGAA AGGCAATGCG 
CTGTTAGCTC TAAGCAGCCT TGCTGTCGTC GTATCTAGAC ATGAAGCCAG CCTCTCCTCA 
GACTCTGACG GGCTCCTGGA GGTTCAACCT AATTTCCTTT CAATGAAAGA GTGGGTTTCC 
ATGATACTTG ATACACTCTT GGTCATTGTG GATAGCCATT ACCAACCCAG AGGGCAACTT 
CTCTCCTGGT TTTATTATAA GTCCTATTCT GGTGAAAACA CAGCTAGTGC CATTGCCCGT 
TCTGCTGCCG CCACGGCTTT GTCTCTCCTT GTGCCAGTTT TCATTATCTC TTGCAAAGAG 
AAGGTTGAGG AAATCCTGAA CATGCTGACT GCCAGGTTAC CTGGGAAACC AAGTGCTGAT 
GAGTCTCAAG CCGTGCAAAT CCACATGGGC CTTGCTTTAG GGATGTTTCT CTCTCGCTTG 
TGTGAAGAGA AACTCAGTGA TATATGTGGC CAAGAGATGA ACCTTCTTCT GATGAAGTCG 
TTGGATGCCC TGGAAAATTG CTGCTTTGAC ACTAGTCTTG AATACAACAC GGGCTGTATA 
TTGGGAGTTG GACTTGTTCT GTCCCTCATG AGTCACAGCA GCCAAATGCA GTCCTGCGTT 
CACGTGACAG CATTGCTTCG GAAGCTGTCT GCATACCTAG ATGACAGTGG GAGCCAGAGC 
AGAACGTTTC AGGAGGTCCT TGCCTACACA CTTAGCTGTG TATGTACATC AGCGTTCAGT 
GCTGGAATTA TTGAGGCTAC AGAGGCTGAG GATGTTATGA ACAAGCTTCG ACTGTTAGTG 
GAGAATAGCC AGCAGACTTC AGGTTTTGCC CTGGCTTTAG GAAACGTAGT TCATGGATTG 
TCTGTGTGTG GACATGGAAA AGCTGAAGAC TTGGGCAGCA AACTACTCCC TGCCTGGATC 
AGAATTGTTC TAACAGAGGG CACTCCCACA ATGCTTTGTC TGGCAGCTCT TCATGGCATG 
GTGGCCCTGG TAGGCTCTGA AGGGGATGTA ATGCAGCTGA AATCAGAAGC CATCCAGACC 
TCTCATTTTC AAGGCAGACT TAATGAAGTC ATTAGAACCT TAACTCAGGT CATTAGTGTC 
TCTGGGGTGA TTGGTCTCCA GTCAAATGCA GTCTGGCTTC TTGGACATCT TCATCTATCT 
ACTCTATCCT CAAGTCAAAG TAGAGCCTCT GTTCCTACTG ACTATAGCTA TTTGCCTGAA 
AGCAGTTTTA TTGGAGCAGC TATTGGCTTC TTCATTACAG GAGGAAAAAA AGGTCCTGAA 
TCTGTGCCTC CTTCCCTTCT TAAAGTAGTG ATGAAACCCA TAGCAACTGT TGGAGAAAGC 
TACCAATATC CTCCTGTGAA CTGGGCTGCA CTTCTCTCTC CACTTATGAG GCTAAATTTT 
GGTGAAGAGA TCCAGCAACT GTGCCTTGAA ATTATGGTGA CCCAGGCACA GTCATCCCAG 
AATGCAGCTG CACTATTGGG CTTGTGGGTG ACACCACCAC TGATCCACAG TCTGAGTCTG 
AATACCAAGA GATATCTCCT GATATCTGCA CCTCTGTGGA TAAAACACAT CTCTGATGAA 
CAGATCCTGG GTTTTGTTGA AAATTTAATG GTGGCAGTTT TTAAAGCAGC TTCCCCACTT 
GGAAATCCTG AGCTATGCCC GAGTGCTTTA CAGGGTCTGA GCCAGGCCAT GAAACTGCCC 
AGCCCTGCTC ACCACCTCTG GAGTCTGCTC TCTGAAGCTA CTGGGAAAAT TTTTGACCTC 
CTGCCAAATA AGATTCGGAG AAAGGATCTA GAGCTGTATA TCAGCATAGC AAAATGCCTC 
TTAGAAATGA CAGATGATGA TGCCAATCGG ATCGCCCAGG TTACTAAGAG CAACATAGAA 
AAGGCTGCCT TTGTCAAACT GTACTTAGTC TCTCAAGGAC GATTCCCCTT GGTGAACCTG 
ACCGATATGC TGAGTGTTGC TGTGCAGCAC CGTGAGAAAG AGGTGTTGGC CTGGATGATT 
CTGCACAGCT TATACCAGGC ACGGATTGTG AGCCATGCCA ACACGGGCGT TTTGAAGAGA 
ATGGAGTGGC TCTTGGAACT GATGGGTTAT ATTAGAAATG TTGCGTACCA GTCAACATCC 
TTTCAGAATA CGGCTCTTGA TGAGGCTTTG GACTTCTTCT TGCTGATATT TGCAACCGCA 
GTGGTTGCAT GGGCCGACCA CACTGCCCCT CTCCTCCTTG GTCTCAGTGC CAGTTGGTTG 
CCATGGCATC AGGAGAATGG CCCGGCTGGG CCAGTACCAA GCTTCCTTGG CAGGAGTCCA 
ATGCACAGGG TCACTGTGCA GGAGGTTCTC ACTCTCCTTC CCAATAGCAT GGCTCTGCTG 
CTGCAGAAAG AGCCATGGAA GGAAGAGACC CAGAAGTTCA TTGACTGGCT ATTCAGCATC 
ATGGAAAGCC CTAAAGAAGC CCTCTCAGCA AAGTCCAGGG ATCTTTTGAA AGCCACCCTG 
CTGTCCTTGA GAGTTCTCCC AGAGTTTAAG AAGAAAGCTG TATGGACCAG AGCGTATGGT 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FOCAD ( XM_009244395.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009242670.1 Pongo abelii focadhesin (FOCAD), transcript variant X7, mRNA.

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CloneID	OSa07887
Clone ID Related Accession (Same CDS sequence)	XM_009244393.1 , XM_009244395.1 , XM_009244392.1 , XM_009244394.1
Accession Version	XM_009244395.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-22
Organism	Pongo abelii(Sumatran orangutan)
Product	focadhesin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002883424.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pongo abelii Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGATG ATATCAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA ATCACAGGCT 
GCGGGTCATC TTATTGCTGC AGTCCTAAAG GAGAATGGTT TTTCAGAAAA GATTTACCAA 
TCTACAAATC AGACTCCTGC TTTGAACTTG CTGTGGGAGA AGTGTTGCAG TGACAATGTA 
GTGGTTCGAA CAGCCTGCTG TGAAGGTCTG GTGGCACTCG TTGCTCAGGA TCATGCAGAG 
TTCAGCTATG TTCTCAATGG GATACTCAAC TTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCATTATGAA ATTACTACAA ATGCAAGCTC TTAAGGAAGG ACAAGGTGGG 
GAAAAGAATA TTCAGAGGGT ATATACCATT AGAAATCATC CTCATCCTTT GATCACTGTG 
CTTGAACACA GACCTGATTG TTGGCCAGTG TTTTTGCAGC AGCTGACAGC ATTTTTCCAG 
CAGTGCCCTG AAAGGTCAGA AGTTTCATGC ATTCAAATAA TGGCACCATT TCTGTGGTAT 
CTGTATTGTG AACCATCTCA GTTACAAGAA TATGCTAAAC TCCGACTAGC CCTGCTGAAA 
GTCTTACTTC AACCCCAGGT TCTTTGTGAC AAGGATCAAC CATCAATACT GCAACAGCAG 
ATACTTCAAC TGTGTTGTGA CATAGTTCCA TGTTTGCAGA TAAAAGATTT GATACAGACA 
ACAGAAGCGA TGATGTTTAT TGAGGAAGTA TATTTAAGCC TTTTGCGTCA TCCTGTTTTC 
TGGAAAATTC AGCTTACCCA GATGACTCTT CAGCTGCTGT GTGTCGGTGA AGTCAGCTTA 
AAGATAACTG GTGAATGTTC ATCTTCAATT CACCTCTTAG AGCACAGTGT TGAACTTCTG 
AAGGAGGATT TTCCTGTTGA ACTGGTCATA ATTGGAATAG CTTTACTACT TCTACAGACT 
CCAGCAAGTC AGCAGAAGCC AATCTTAAAT CTAGCTTTGA AGCTCTTCTC TGTTACTGAG 
GATCAGAAAA TCCCAAAGTC CTCTCTGCTG CTAGTGATGC CAATTCTGCA GATACTGTCT 
TCTACTGCCT TGGAAGACTG TATATCTGTG GATGAAGAAG GTCCCTCTAG GCAGCAGTTG 
GCTCTAAACC TTTTGGAAAT GATACAGCAG GAATGTTACA GAGATGACCA CCAAAAGCTC 
TCCTACAAGC TTGTGTGCCC TGTAACCAGT ATGTATGGTT CAGTATTTAC AGCCTGGAGG 
ATTCTTGAAG TAATGACAGA CTCCTCTGCT GCAAGTGACT GGTTGGCTTC AGTAGAGTCA 
TTGCTTCCTA TTACTACTGT GATCCCTGTG CCTGCCTTTC TTCTGCTGGC TCACCTCCTT 
GTTGAAGACA AAGGACAAAA TCTTCACCAA ATACTCAAGG TCACTACAGA ATTAGCCCAA 
GCAGATTCCT CCCAGGTGCC AAATCTGATT CCAGTTTTGA TGTTCAAATT GGGAAGACCA 
CTGGAACCTA TATTATATAA TGATATATTG TATACTTTAC CTAAGCTTGG TGTTCACAAG 
GTGTGTATAG GACAAATTCT ACGAGTAATA CAACTGCTTG GAACCACACC ACGACTAAGA 
GCTGTCACTT TGCGCTTGCT GACATCTTTG TGGGAAAAGC AGGACCGAGT CTATCCTGAA 
CTGCAGCGTT TCATGGCTGT GTCTGATGTA CCTTCTCTTT CGGTGGGCAA GGAAGTCCAA 
TGGGAGAAAC TGATTGCAAA AGCAGCATCA ATCAGAGATA TATGTAAGCA GAGGCCATAT 
CAACATGGTG CAGATATGCT GGCAGCTATT TCTCAGGTGT TGAATGAATG CACCAAGCCT 
GATCAAGCTA CTCCAGCAGC CTTGGTATTA CAAGGTCTTC ATGCACTCTG TCAAGCTGAG 
GTTGTTTGCA TTCGCTCCAC TTGGAATGCT CTCTCTCCAA AGCTGAGTTG TGACACAAGA 
CCTCTCATTC TGAAGACACT GAGTGAACTA TTTTCTCTAG TTCCTTCCTT AACAGTCAAT 
ACAACTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAC TCATACTCAA 
AACAAGGACC CAATTGTGGC AAATGCTGCA TATAGATCCC TGGCCAATTT TAGTGCAGGA 
GAACACACCA TTCTTCATCT GCCTGAAAAG ATAAGACCAG AAATTCCCAT TCCTGAAGAG 
TTAGATGATG ATGAAGATGA TGAGGATGTG GATCTTTCAG TTCCTGGCTC TTGCTATCTC 
AAACTGTTGT CACTCACTCC CCCTTTGGTT TTACCAGCTT TGGAGGAATT TTTTACATCA 
CTTGTGAAGC AAGAAATGGT GAATATGCCT CGTGGGATAT ATCACTCTGC ATTAAAAGGA 
GGTGTCCGCT CAGACCAAGG AAAGACTGTA GCAGGAATCC CCAATTTTAT ATTGAAAATG 
TATGAAACAA ACAAGCAACC AGGATTGAAA CCTGGCCTTG CAGGTGGTAT GTTATTTTGC 
TATGATGTTT CCATGTATCA GAGTAAAGAT GGAAAACCAT TGAATAGACT GATGGCCAGC 
AGAGGGCGAA GTTTCAAGCA GACATCACTT GCTCTTGTAC ATGAGGTTCA TATCCAGCTT 
TCAGAGTGGC ACCGTGCGAT TTTTCTTCCA CAGGCCTGGC TTGCATACAT GAATCGAGCT 
TATCATGCCA TTTTACAGGG AAGACTAGGA GAGCTGGAGT TGCAGTTAAA ACATGGAAAA 
GAAGAACCTG AGGAGGTGCA GTACAAAAAA AGCACAGCTT GGCTCTGGGT TAGAGACATG 
CTGACTGATG AGATCACCAA GGCAGCTGCA AAGGAGAGTC CGGTAGTGAA AGGCAATGCG 
CTGTTAGCTC TAAGCAGCCT TGCTGTCGTC GTATCTAGAC ATGAAGCCAG CCTCTCCTCA 
GACTCTGACG GGCTCCTGGA GGTTCAACCT AATTTCCTTT CAATGAAAGA GTGGGTTTCC 
ATGATACTTG ATACACTCTT GGTCATTGTG GATAGCCATT ACCAACCCAG AGGGCAACTT 
CTCTCCTGGT TTTATTATAA GTCCTATTCT GGTGAAAACA CAGCTAGTGC CATTGCCCGT 
TCTGCTGCCG CCACGGCTTT GTCTCTCCTT GTGCCAGTTT TCATTATCTC TTGCAAAGAG 
AAGGTTGAGG AAATCCTGAA CATGCTGACT GCCAGGTTAC CTGGGAAACC AAGTGCTGAT 
GAGTCTCAAG CCGTGCAAAT CCACATGGGC CTTGCTTTAG GGATGTTTCT CTCTCGCTTG 
TGTGAAGAGA AACTCAGTGA TATATGTGGC CAAGAGATGA ACCTTCTTCT GATGAAGTCG 
TTGGATGCCC TGGAAAATTG CTGCTTTGAC ACTAGTCTTG AATACAACAC GGGCTGTATA 
TTGGGAGTTG GACTTGTTCT GTCCCTCATG AGTCACAGCA GCCAAATGCA GTCCTGCGTT 
CACGTGACAG CATTGCTTCG GAAGCTGTCT GCATACCTAG ATGACAGTGG GAGCCAGAGC 
AGAACGTTTC AGGAGGTCCT TGCCTACACA CTTAGCTGTG TATGTACATC AGCGTTCAGT 
GCTGGAATTA TTGAGGCTAC AGAGGCTGAG GATGTTATGA ACAAGCTTCG ACTGTTAGTG 
GAGAATAGCC AGCAGACTTC AGGTTTTGCC CTGGCTTTAG GAAACGTAGT TCATGGATTG 
TCTGTGTGTG GACATGGAAA AGCTGAAGAC TTGGGCAGCA AACTACTCCC TGCCTGGATC 
AGAATTGTTC TAACAGAGGG CACTCCCACA ATGCTTTGTC TGGCAGCTCT TCATGGCATG 
GTGGCCCTGG TAGGCTCTGA AGGGGATGTA ATGCAGCTGA AATCAGAAGC CATCCAGACC 
TCTCATTTTC AAGGCAGACT TAATGAAGTC ATTAGAACCT TAACTCAGGT CATTAGTGTC 
TCTGGGGTGA TTGGTCTCCA GTCAAATGCA GTCTGGCTTC TTGGACATCT TCATCTATCT 
ACTCTATCCT CAAGTCAAAG TAGAGCCTCT GTTCCTACTG ACTATAGCTA TTTGCCTGAA 
AGCAGTTTTA TTGGAGCAGC TATTGGCTTC TTCATTACAG GAGGAAAAAA AGGTCCTGAA 
TCTGTGCCTC CTTCCCTTCT TAAAGTAGTG ATGAAACCCA TAGCAACTGT TGGAGAAAGC 
TACCAATATC CTCCTGTGAA CTGGGCTGCA CTTCTCTCTC CACTTATGAG GCTAAATTTT 
GGTGAAGAGA TCCAGCAACT GTGCCTTGAA ATTATGGTGA CCCAGGCACA GTCATCCCAG 
AATGCAGCTG CACTATTGGG CTTGTGGGTG ACACCACCAC TGATCCACAG TCTGAGTCTG 
AATACCAAGA GATATCTCCT GATATCTGCA CCTCTGTGGA TAAAACACAT CTCTGATGAA 
CAGATCCTGG GTTTTGTTGA AAATTTAATG GTGGCAGTTT TTAAAGCAGC TTCCCCACTT 
GGAAATCCTG AGCTATGCCC GAGTGCTTTA CAGGGTCTGA GCCAGGCCAT GAAACTGCCC 
AGCCCTGCTC ACCACCTCTG GAGTCTGCTC TCTGAAGCTA CTGGGAAAAT TTTTGACCTC 
CTGCCAAATA AGATTCGGAG AAAGGATCTA GAGCTGTATA TCAGCATAGC AAAATGCCTC 
TTAGAAATGA CAGATGATGA TGCCAATCGG ATCGCCCAGG TTACTAAGAG CAACATAGAA 
AAGGCTGCCT TTGTCAAACT GTACTTAGTC TCTCAAGGAC GATTCCCCTT GGTGAACCTG 
ACCGATATGC TGAGTGTTGC TGTGCAGCAC CGTGAGAAAG AGGTGTTGGC CTGGATGATT 
CTGCACAGCT TATACCAGGC ACGGATTGTG AGCCATGCCA ACACGGGCGT TTTGAAGAGA 
ATGGAGTGGC TCTTGGAACT GATGGGTTAT ATTAGAAATG TTGCGTACCA GTCAACATCC 
TTTCAGAATA CGGCTCTTGA TGAGGCTTTG GACTTCTTCT TGCTGATATT TGCAACCGCA 
GTGGTTGCAT GGGCCGACCA CACTGCCCCT CTCCTCCTTG GTCTCAGTGC CAGTTGGTTG 
CCATGGCATC AGGAGAATGG CCCGGCTGGG CCAGTACCAA GCTTCCTTGG CAGGAGTCCA 
ATGCACAGGG TCACTGTGCA GGAGGTTCTC ACTCTCCTTC CCAATAGCAT GGCTCTGCTG 
CTGCAGAAAG AGCCATGGAA GGAAGAGACC CAGAAGTTCA TTGACTGGCT ATTCAGCATC 
ATGGAAAGCC CTAAAGAAGC CCTCTCAGCA AAGTCCAGGG ATCTTTTGAA AGCCACCCTG 
CTGTCCTTGA GAGTTCTCCC AGAGTTTAAG AAGAAAGCTG TATGGACCAG AGCGTATGGT 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009242670.1
CDS	149..5554
Translation	MSDDIRKRFEFPNSLIQSQAAGHLIAAVLKENGFSEKIYQSTNQTPALNLLWEKCCSDNVVVRTACCEGLVALVAQDHAEFSYVLNGILNLIPSTRNTHGLIKAIMKLLQMQALKEGQGGEKNIQRVYTIRNHPHPLITVLEHRPDCWPVFLQQLTAFFQQCPERSEVSCIQIMAPFLWYLYCEPSQLQEYAKLRLALLKVLLQPQVLCDKDQPSILQQQILQLCCDIVPCLQIKDLIQTTEAMMFIEEVYLSLLRHPVFWKIQLTQMTLQLLCVGEVSLKITGECSSSIHLLEHSVELLKEDFPVELVIIGIALLLLQTPASQQKPILNLALKLFSVTEDQKIPKSSLLLVMPILQILSSTALEDCISVDEEGPSRQQLALNLLEMIQQECYRDDHQKLSYKLVCPVTSMYGSVFTAWRILEVMTDSSAASDWLASVESLLPITTVIPVPAFLLLAHLLVEDKGQNLHQILKVTTELAQADSSQVPNLIPVLMFKLGRPLEPILYNDILYTLPKLGVHKVCIGQILRVIQLLGTTPRLRAVTLRLLTSLWEKQDRVYPELQRFMAVSDVPSLSVGKEVQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQATPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDTRPLILKTLSELFSLVPSLTVNTTEYENFKVQVLSFLWTHTQNKDPIVANAAYRSLANFSAGEHTILHLPEKIRPEIPIPEELDDDEDDEDVDLSVPGSCYLKLLSLTPPLVLPALEEFFTSLVKQEMVNMPRGIYHSALKGGVRSDQGKTVAGIPNFILKMYETNKQPGLKPGLAGGMLFCYDVSMYQSKDGKPLNRLMASRGRSFKQTSLALVHEVHIQLSEWHRAIFLPQAWLAYMNRAYHAILQGRLGELELQLKHGKEEPEEVQYKKSTAWLWVRDMLTDEITKAAAKESPVVKGNALLALSSLAVVVSRHEASLSSDSDGLLEVQPNFLSMKEWVSMILDTLLVIVDSHYQPRGQLLSWFYYKSYSGENTASAIARSAAATALSLLVPVFIISCKEKVEEILNMLTARLPGKPSADESQAVQIHMGLALGMFLSRLCEEKLSDICGQEMNLLLMKSLDALENCCFDTSLEYNTGCILGVGLVLSLMSHSSQMQSCVHVTALLRKLSAYLDDSGSQSRTFQEVLAYTLSCVCTSAFSAGIIEATEAEDVMNKLRLLVENSQQTSGFALALGNVVHGLSVCGHGKAEDLGSKLLPAWIRIVLTEGTPTMLCLAALHGMVALVGSEGDVMQLKSEAIQTSHFQGRLNEVIRTLTQVISVSGVIGLQSNAVWLLGHLHLSTLSSSQSRASVPTDYSYLPESSFIGAAIGFFITGGKKGPESVPPSLLKVVMKPIATVGESYQYPPVNWAALLSPLMRLNFGEEIQQLCLEIMVTQAQSSQNAAALLGLWVTPPLIHSLSLNTKRYLLISAPLWIKHISDEQILGFVENLMVAVFKAASPLGNPELCPSALQGLSQAMKLPSPAHHLWSLLSEATGKIFDLLPNKIRRKDLELYISIAKCLLEMTDDDANRIAQVTKSNIEKAAFVKLYLVSQGRFPLVNLTDMLSVAVQHREKEVLAWMILHSLYQARIVSHANTGVLKRMEWLLELMGYIRNVAYQSTSFQNTALDEALDFFLLIFATAVVAWADHTAPLLLGLSASWLPWHQENGPAGPVPSFLGRSPMHRVTVQEVLTLLPNSMALLLQKEPWKEETQKFIDWLFSIMESPKEALSAKSRDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_009244395.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii focadhesin (FOCAD), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009244395.1

ORF Insert Sequence:

ATGTCAGATG ATATCAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA ATCACAGGCT 
GCGGGTCATC TTATTGCTGC AGTCCTAAAG GAGAATGGTT TTTCAGAAAA GATTTACCAA 
TCTACAAATC AGACTCCTGC TTTGAACTTG CTGTGGGAGA AGTGTTGCAG TGACAATGTA 
GTGGTTCGAA CAGCCTGCTG TGAAGGTCTG GTGGCACTCG TTGCTCAGGA TCATGCAGAG 
TTCAGCTATG TTCTCAATGG GATACTCAAC TTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCATTATGAA ATTACTACAA ATGCAAGCTC TTAAGGAAGG ACAAGGTGGG 
GAAAAGAATA TTCAGAGGGT ATATACCATT AGAAATCATC CTCATCCTTT GATCACTGTG 
CTTGAACACA GACCTGATTG TTGGCCAGTG TTTTTGCAGC AGCTGACAGC ATTTTTCCAG 
CAGTGCCCTG AAAGGTCAGA AGTTTCATGC ATTCAAATAA TGGCACCATT TCTGTGGTAT 
CTGTATTGTG AACCATCTCA GTTACAAGAA TATGCTAAAC TCCGACTAGC CCTGCTGAAA 
GTCTTACTTC AACCCCAGGT TCTTTGTGAC AAGGATCAAC CATCAATACT GCAACAGCAG 
ATACTTCAAC TGTGTTGTGA CATAGTTCCA TGTTTGCAGA TAAAAGATTT GATACAGACA 
ACAGAAGCGA TGATGTTTAT TGAGGAAGTA TATTTAAGCC TTTTGCGTCA TCCTGTTTTC 
TGGAAAATTC AGCTTACCCA GATGACTCTT CAGCTGCTGT GTGTCGGTGA AGTCAGCTTA 
AAGATAACTG GTGAATGTTC ATCTTCAATT CACCTCTTAG AGCACAGTGT TGAACTTCTG 
AAGGAGGATT TTCCTGTTGA ACTGGTCATA ATTGGAATAG CTTTACTACT TCTACAGACT 
CCAGCAAGTC AGCAGAAGCC AATCTTAAAT CTAGCTTTGA AGCTCTTCTC TGTTACTGAG 
GATCAGAAAA TCCCAAAGTC CTCTCTGCTG CTAGTGATGC CAATTCTGCA GATACTGTCT 
TCTACTGCCT TGGAAGACTG TATATCTGTG GATGAAGAAG GTCCCTCTAG GCAGCAGTTG 
GCTCTAAACC TTTTGGAAAT GATACAGCAG GAATGTTACA GAGATGACCA CCAAAAGCTC 
TCCTACAAGC TTGTGTGCCC TGTAACCAGT ATGTATGGTT CAGTATTTAC AGCCTGGAGG 
ATTCTTGAAG TAATGACAGA CTCCTCTGCT GCAAGTGACT GGTTGGCTTC AGTAGAGTCA 
TTGCTTCCTA TTACTACTGT GATCCCTGTG CCTGCCTTTC TTCTGCTGGC TCACCTCCTT 
GTTGAAGACA AAGGACAAAA TCTTCACCAA ATACTCAAGG TCACTACAGA ATTAGCCCAA 
GCAGATTCCT CCCAGGTGCC AAATCTGATT CCAGTTTTGA TGTTCAAATT GGGAAGACCA 
CTGGAACCTA TATTATATAA TGATATATTG TATACTTTAC CTAAGCTTGG TGTTCACAAG 
GTGTGTATAG GACAAATTCT ACGAGTAATA CAACTGCTTG GAACCACACC ACGACTAAGA 
GCTGTCACTT TGCGCTTGCT GACATCTTTG TGGGAAAAGC AGGACCGAGT CTATCCTGAA 
CTGCAGCGTT TCATGGCTGT GTCTGATGTA CCTTCTCTTT CGGTGGGCAA GGAAGTCCAA 
TGGGAGAAAC TGATTGCAAA AGCAGCATCA ATCAGAGATA TATGTAAGCA GAGGCCATAT 
CAACATGGTG CAGATATGCT GGCAGCTATT TCTCAGGTGT TGAATGAATG CACCAAGCCT 
GATCAAGCTA CTCCAGCAGC CTTGGTATTA CAAGGTCTTC ATGCACTCTG TCAAGCTGAG 
GTTGTTTGCA TTCGCTCCAC TTGGAATGCT CTCTCTCCAA AGCTGAGTTG TGACACAAGA 
CCTCTCATTC TGAAGACACT GAGTGAACTA TTTTCTCTAG TTCCTTCCTT AACAGTCAAT 
ACAACTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAC TCATACTCAA 
AACAAGGACC CAATTGTGGC AAATGCTGCA TATAGATCCC TGGCCAATTT TAGTGCAGGA 
GAACACACCA TTCTTCATCT GCCTGAAAAG ATAAGACCAG AAATTCCCAT TCCTGAAGAG 
TTAGATGATG ATGAAGATGA TGAGGATGTG GATCTTTCAG TTCCTGGCTC TTGCTATCTC 
AAACTGTTGT CACTCACTCC CCCTTTGGTT TTACCAGCTT TGGAGGAATT TTTTACATCA 
CTTGTGAAGC AAGAAATGGT GAATATGCCT CGTGGGATAT ATCACTCTGC ATTAAAAGGA 
GGTGTCCGCT CAGACCAAGG AAAGACTGTA GCAGGAATCC CCAATTTTAT ATTGAAAATG 
TATGAAACAA ACAAGCAACC AGGATTGAAA CCTGGCCTTG CAGGTGGTAT GTTATTTTGC 
TATGATGTTT CCATGTATCA GAGTAAAGAT GGAAAACCAT TGAATAGACT GATGGCCAGC 
AGAGGGCGAA GTTTCAAGCA GACATCACTT GCTCTTGTAC ATGAGGTTCA TATCCAGCTT 
TCAGAGTGGC ACCGTGCGAT TTTTCTTCCA CAGGCCTGGC TTGCATACAT GAATCGAGCT 
TATCATGCCA TTTTACAGGG AAGACTAGGA GAGCTGGAGT TGCAGTTAAA ACATGGAAAA 
GAAGAACCTG AGGAGGTGCA GTACAAAAAA AGCACAGCTT GGCTCTGGGT TAGAGACATG 
CTGACTGATG AGATCACCAA GGCAGCTGCA AAGGAGAGTC CGGTAGTGAA AGGCAATGCG 
CTGTTAGCTC TAAGCAGCCT TGCTGTCGTC GTATCTAGAC ATGAAGCCAG CCTCTCCTCA 
GACTCTGACG GGCTCCTGGA GGTTCAACCT AATTTCCTTT CAATGAAAGA GTGGGTTTCC 
ATGATACTTG ATACACTCTT GGTCATTGTG GATAGCCATT ACCAACCCAG AGGGCAACTT 
CTCTCCTGGT TTTATTATAA GTCCTATTCT GGTGAAAACA CAGCTAGTGC CATTGCCCGT 
TCTGCTGCCG CCACGGCTTT GTCTCTCCTT GTGCCAGTTT TCATTATCTC TTGCAAAGAG 
AAGGTTGAGG AAATCCTGAA CATGCTGACT GCCAGGTTAC CTGGGAAACC AAGTGCTGAT 
GAGTCTCAAG CCGTGCAAAT CCACATGGGC CTTGCTTTAG GGATGTTTCT CTCTCGCTTG 
TGTGAAGAGA AACTCAGTGA TATATGTGGC CAAGAGATGA ACCTTCTTCT GATGAAGTCG 
TTGGATGCCC TGGAAAATTG CTGCTTTGAC ACTAGTCTTG AATACAACAC GGGCTGTATA 
TTGGGAGTTG GACTTGTTCT GTCCCTCATG AGTCACAGCA GCCAAATGCA GTCCTGCGTT 
CACGTGACAG CATTGCTTCG GAAGCTGTCT GCATACCTAG ATGACAGTGG GAGCCAGAGC 
AGAACGTTTC AGGAGGTCCT TGCCTACACA CTTAGCTGTG TATGTACATC AGCGTTCAGT 
GCTGGAATTA TTGAGGCTAC AGAGGCTGAG GATGTTATGA ACAAGCTTCG ACTGTTAGTG 
GAGAATAGCC AGCAGACTTC AGGTTTTGCC CTGGCTTTAG GAAACGTAGT TCATGGATTG 
TCTGTGTGTG GACATGGAAA AGCTGAAGAC TTGGGCAGCA AACTACTCCC TGCCTGGATC 
AGAATTGTTC TAACAGAGGG CACTCCCACA ATGCTTTGTC TGGCAGCTCT TCATGGCATG 
GTGGCCCTGG TAGGCTCTGA AGGGGATGTA ATGCAGCTGA AATCAGAAGC CATCCAGACC 
TCTCATTTTC AAGGCAGACT TAATGAAGTC ATTAGAACCT TAACTCAGGT CATTAGTGTC 
TCTGGGGTGA TTGGTCTCCA GTCAAATGCA GTCTGGCTTC TTGGACATCT TCATCTATCT 
ACTCTATCCT CAAGTCAAAG TAGAGCCTCT GTTCCTACTG ACTATAGCTA TTTGCCTGAA 
AGCAGTTTTA TTGGAGCAGC TATTGGCTTC TTCATTACAG GAGGAAAAAA AGGTCCTGAA 
TCTGTGCCTC CTTCCCTTCT TAAAGTAGTG ATGAAACCCA TAGCAACTGT TGGAGAAAGC 
TACCAATATC CTCCTGTGAA CTGGGCTGCA CTTCTCTCTC CACTTATGAG GCTAAATTTT 
GGTGAAGAGA TCCAGCAACT GTGCCTTGAA ATTATGGTGA CCCAGGCACA GTCATCCCAG 
AATGCAGCTG CACTATTGGG CTTGTGGGTG ACACCACCAC TGATCCACAG TCTGAGTCTG 
AATACCAAGA GATATCTCCT GATATCTGCA CCTCTGTGGA TAAAACACAT CTCTGATGAA 
CAGATCCTGG GTTTTGTTGA AAATTTAATG GTGGCAGTTT TTAAAGCAGC TTCCCCACTT 
GGAAATCCTG AGCTATGCCC GAGTGCTTTA CAGGGTCTGA GCCAGGCCAT GAAACTGCCC 
AGCCCTGCTC ACCACCTCTG GAGTCTGCTC TCTGAAGCTA CTGGGAAAAT TTTTGACCTC 
CTGCCAAATA AGATTCGGAG AAAGGATCTA GAGCTGTATA TCAGCATAGC AAAATGCCTC 
TTAGAAATGA CAGATGATGA TGCCAATCGG ATCGCCCAGG TTACTAAGAG CAACATAGAA 
AAGGCTGCCT TTGTCAAACT GTACTTAGTC TCTCAAGGAC GATTCCCCTT GGTGAACCTG 
ACCGATATGC TGAGTGTTGC TGTGCAGCAC CGTGAGAAAG AGGTGTTGGC CTGGATGATT 
CTGCACAGCT TATACCAGGC ACGGATTGTG AGCCATGCCA ACACGGGCGT TTTGAAGAGA 
ATGGAGTGGC TCTTGGAACT GATGGGTTAT ATTAGAAATG TTGCGTACCA GTCAACATCC 
TTTCAGAATA CGGCTCTTGA TGAGGCTTTG GACTTCTTCT TGCTGATATT TGCAACCGCA 
GTGGTTGCAT GGGCCGACCA CACTGCCCCT CTCCTCCTTG GTCTCAGTGC CAGTTGGTTG 
CCATGGCATC AGGAGAATGG CCCGGCTGGG CCAGTACCAA GCTTCCTTGG CAGGAGTCCA 
ATGCACAGGG TCACTGTGCA GGAGGTTCTC ACTCTCCTTC CCAATAGCAT GGCTCTGCTG 
CTGCAGAAAG AGCCATGGAA GGAAGAGACC CAGAAGTTCA TTGACTGGCT ATTCAGCATC 
ATGGAAAGCC CTAAAGAAGC CCTCTCAGCA AAGTCCAGGG ATCTTTTGAA AGCCACCCTG 
CTGTCCTTGA GAGTTCTCCC AGAGTTTAAG AAGAAAGCTG TATGGACCAG AGCGTATGGT 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FOCAD ( XM_009244392.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009242667.1 Pongo abelii focadhesin (FOCAD), transcript variant X4, mRNA.

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CloneID	OSa07887
Clone ID Related Accession (Same CDS sequence)	XM_009244393.1 , XM_009244395.1 , XM_009244392.1 , XM_009244394.1
Accession Version	XM_009244392.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-22
Organism	Pongo abelii(Sumatran orangutan)
Product	focadhesin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002883424.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pongo abelii Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGATG ATATCAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA ATCACAGGCT 
GCGGGTCATC TTATTGCTGC AGTCCTAAAG GAGAATGGTT TTTCAGAAAA GATTTACCAA 
TCTACAAATC AGACTCCTGC TTTGAACTTG CTGTGGGAGA AGTGTTGCAG TGACAATGTA 
GTGGTTCGAA CAGCCTGCTG TGAAGGTCTG GTGGCACTCG TTGCTCAGGA TCATGCAGAG 
TTCAGCTATG TTCTCAATGG GATACTCAAC TTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCATTATGAA ATTACTACAA ATGCAAGCTC TTAAGGAAGG ACAAGGTGGG 
GAAAAGAATA TTCAGAGGGT ATATACCATT AGAAATCATC CTCATCCTTT GATCACTGTG 
CTTGAACACA GACCTGATTG TTGGCCAGTG TTTTTGCAGC AGCTGACAGC ATTTTTCCAG 
CAGTGCCCTG AAAGGTCAGA AGTTTCATGC ATTCAAATAA TGGCACCATT TCTGTGGTAT 
CTGTATTGTG AACCATCTCA GTTACAAGAA TATGCTAAAC TCCGACTAGC CCTGCTGAAA 
GTCTTACTTC AACCCCAGGT TCTTTGTGAC AAGGATCAAC CATCAATACT GCAACAGCAG 
ATACTTCAAC TGTGTTGTGA CATAGTTCCA TGTTTGCAGA TAAAAGATTT GATACAGACA 
ACAGAAGCGA TGATGTTTAT TGAGGAAGTA TATTTAAGCC TTTTGCGTCA TCCTGTTTTC 
TGGAAAATTC AGCTTACCCA GATGACTCTT CAGCTGCTGT GTGTCGGTGA AGTCAGCTTA 
AAGATAACTG GTGAATGTTC ATCTTCAATT CACCTCTTAG AGCACAGTGT TGAACTTCTG 
AAGGAGGATT TTCCTGTTGA ACTGGTCATA ATTGGAATAG CTTTACTACT TCTACAGACT 
CCAGCAAGTC AGCAGAAGCC AATCTTAAAT CTAGCTTTGA AGCTCTTCTC TGTTACTGAG 
GATCAGAAAA TCCCAAAGTC CTCTCTGCTG CTAGTGATGC CAATTCTGCA GATACTGTCT 
TCTACTGCCT TGGAAGACTG TATATCTGTG GATGAAGAAG GTCCCTCTAG GCAGCAGTTG 
GCTCTAAACC TTTTGGAAAT GATACAGCAG GAATGTTACA GAGATGACCA CCAAAAGCTC 
TCCTACAAGC TTGTGTGCCC TGTAACCAGT ATGTATGGTT CAGTATTTAC AGCCTGGAGG 
ATTCTTGAAG TAATGACAGA CTCCTCTGCT GCAAGTGACT GGTTGGCTTC AGTAGAGTCA 
TTGCTTCCTA TTACTACTGT GATCCCTGTG CCTGCCTTTC TTCTGCTGGC TCACCTCCTT 
GTTGAAGACA AAGGACAAAA TCTTCACCAA ATACTCAAGG TCACTACAGA ATTAGCCCAA 
GCAGATTCCT CCCAGGTGCC AAATCTGATT CCAGTTTTGA TGTTCAAATT GGGAAGACCA 
CTGGAACCTA TATTATATAA TGATATATTG TATACTTTAC CTAAGCTTGG TGTTCACAAG 
GTGTGTATAG GACAAATTCT ACGAGTAATA CAACTGCTTG GAACCACACC ACGACTAAGA 
GCTGTCACTT TGCGCTTGCT GACATCTTTG TGGGAAAAGC AGGACCGAGT CTATCCTGAA 
CTGCAGCGTT TCATGGCTGT GTCTGATGTA CCTTCTCTTT CGGTGGGCAA GGAAGTCCAA 
TGGGAGAAAC TGATTGCAAA AGCAGCATCA ATCAGAGATA TATGTAAGCA GAGGCCATAT 
CAACATGGTG CAGATATGCT GGCAGCTATT TCTCAGGTGT TGAATGAATG CACCAAGCCT 
GATCAAGCTA CTCCAGCAGC CTTGGTATTA CAAGGTCTTC ATGCACTCTG TCAAGCTGAG 
GTTGTTTGCA TTCGCTCCAC TTGGAATGCT CTCTCTCCAA AGCTGAGTTG TGACACAAGA 
CCTCTCATTC TGAAGACACT GAGTGAACTA TTTTCTCTAG TTCCTTCCTT AACAGTCAAT 
ACAACTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAC TCATACTCAA 
AACAAGGACC CAATTGTGGC AAATGCTGCA TATAGATCCC TGGCCAATTT TAGTGCAGGA 
GAACACACCA TTCTTCATCT GCCTGAAAAG ATAAGACCAG AAATTCCCAT TCCTGAAGAG 
TTAGATGATG ATGAAGATGA TGAGGATGTG GATCTTTCAG TTCCTGGCTC TTGCTATCTC 
AAACTGTTGT CACTCACTCC CCCTTTGGTT TTACCAGCTT TGGAGGAATT TTTTACATCA 
CTTGTGAAGC AAGAAATGGT GAATATGCCT CGTGGGATAT ATCACTCTGC ATTAAAAGGA 
GGTGTCCGCT CAGACCAAGG AAAGACTGTA GCAGGAATCC CCAATTTTAT ATTGAAAATG 
TATGAAACAA ACAAGCAACC AGGATTGAAA CCTGGCCTTG CAGGTGGTAT GTTATTTTGC 
TATGATGTTT CCATGTATCA GAGTAAAGAT GGAAAACCAT TGAATAGACT GATGGCCAGC 
AGAGGGCGAA GTTTCAAGCA GACATCACTT GCTCTTGTAC ATGAGGTTCA TATCCAGCTT 
TCAGAGTGGC ACCGTGCGAT TTTTCTTCCA CAGGCCTGGC TTGCATACAT GAATCGAGCT 
TATCATGCCA TTTTACAGGG AAGACTAGGA GAGCTGGAGT TGCAGTTAAA ACATGGAAAA 
GAAGAACCTG AGGAGGTGCA GTACAAAAAA AGCACAGCTT GGCTCTGGGT TAGAGACATG 
CTGACTGATG AGATCACCAA GGCAGCTGCA AAGGAGAGTC CGGTAGTGAA AGGCAATGCG 
CTGTTAGCTC TAAGCAGCCT TGCTGTCGTC GTATCTAGAC ATGAAGCCAG CCTCTCCTCA 
GACTCTGACG GGCTCCTGGA GGTTCAACCT AATTTCCTTT CAATGAAAGA GTGGGTTTCC 
ATGATACTTG ATACACTCTT GGTCATTGTG GATAGCCATT ACCAACCCAG AGGGCAACTT 
CTCTCCTGGT TTTATTATAA GTCCTATTCT GGTGAAAACA CAGCTAGTGC CATTGCCCGT 
TCTGCTGCCG CCACGGCTTT GTCTCTCCTT GTGCCAGTTT TCATTATCTC TTGCAAAGAG 
AAGGTTGAGG AAATCCTGAA CATGCTGACT GCCAGGTTAC CTGGGAAACC AAGTGCTGAT 
GAGTCTCAAG CCGTGCAAAT CCACATGGGC CTTGCTTTAG GGATGTTTCT CTCTCGCTTG 
TGTGAAGAGA AACTCAGTGA TATATGTGGC CAAGAGATGA ACCTTCTTCT GATGAAGTCG 
TTGGATGCCC TGGAAAATTG CTGCTTTGAC ACTAGTCTTG AATACAACAC GGGCTGTATA 
TTGGGAGTTG GACTTGTTCT GTCCCTCATG AGTCACAGCA GCCAAATGCA GTCCTGCGTT 
CACGTGACAG CATTGCTTCG GAAGCTGTCT GCATACCTAG ATGACAGTGG GAGCCAGAGC 
AGAACGTTTC AGGAGGTCCT TGCCTACACA CTTAGCTGTG TATGTACATC AGCGTTCAGT 
GCTGGAATTA TTGAGGCTAC AGAGGCTGAG GATGTTATGA ACAAGCTTCG ACTGTTAGTG 
GAGAATAGCC AGCAGACTTC AGGTTTTGCC CTGGCTTTAG GAAACGTAGT TCATGGATTG 
TCTGTGTGTG GACATGGAAA AGCTGAAGAC TTGGGCAGCA AACTACTCCC TGCCTGGATC 
AGAATTGTTC TAACAGAGGG CACTCCCACA ATGCTTTGTC TGGCAGCTCT TCATGGCATG 
GTGGCCCTGG TAGGCTCTGA AGGGGATGTA ATGCAGCTGA AATCAGAAGC CATCCAGACC 
TCTCATTTTC AAGGCAGACT TAATGAAGTC ATTAGAACCT TAACTCAGGT CATTAGTGTC 
TCTGGGGTGA TTGGTCTCCA GTCAAATGCA GTCTGGCTTC TTGGACATCT TCATCTATCT 
ACTCTATCCT CAAGTCAAAG TAGAGCCTCT GTTCCTACTG ACTATAGCTA TTTGCCTGAA 
AGCAGTTTTA TTGGAGCAGC TATTGGCTTC TTCATTACAG GAGGAAAAAA AGGTCCTGAA 
TCTGTGCCTC CTTCCCTTCT TAAAGTAGTG ATGAAACCCA TAGCAACTGT TGGAGAAAGC 
TACCAATATC CTCCTGTGAA CTGGGCTGCA CTTCTCTCTC CACTTATGAG GCTAAATTTT 
GGTGAAGAGA TCCAGCAACT GTGCCTTGAA ATTATGGTGA CCCAGGCACA GTCATCCCAG 
AATGCAGCTG CACTATTGGG CTTGTGGGTG ACACCACCAC TGATCCACAG TCTGAGTCTG 
AATACCAAGA GATATCTCCT GATATCTGCA CCTCTGTGGA TAAAACACAT CTCTGATGAA 
CAGATCCTGG GTTTTGTTGA AAATTTAATG GTGGCAGTTT TTAAAGCAGC TTCCCCACTT 
GGAAATCCTG AGCTATGCCC GAGTGCTTTA CAGGGTCTGA GCCAGGCCAT GAAACTGCCC 
AGCCCTGCTC ACCACCTCTG GAGTCTGCTC TCTGAAGCTA CTGGGAAAAT TTTTGACCTC 
CTGCCAAATA AGATTCGGAG AAAGGATCTA GAGCTGTATA TCAGCATAGC AAAATGCCTC 
TTAGAAATGA CAGATGATGA TGCCAATCGG ATCGCCCAGG TTACTAAGAG CAACATAGAA 
AAGGCTGCCT TTGTCAAACT GTACTTAGTC TCTCAAGGAC GATTCCCCTT GGTGAACCTG 
ACCGATATGC TGAGTGTTGC TGTGCAGCAC CGTGAGAAAG AGGTGTTGGC CTGGATGATT 
CTGCACAGCT TATACCAGGC ACGGATTGTG AGCCATGCCA ACACGGGCGT TTTGAAGAGA 
ATGGAGTGGC TCTTGGAACT GATGGGTTAT ATTAGAAATG TTGCGTACCA GTCAACATCC 
TTTCAGAATA CGGCTCTTGA TGAGGCTTTG GACTTCTTCT TGCTGATATT TGCAACCGCA 
GTGGTTGCAT GGGCCGACCA CACTGCCCCT CTCCTCCTTG GTCTCAGTGC CAGTTGGTTG 
CCATGGCATC AGGAGAATGG CCCGGCTGGG CCAGTACCAA GCTTCCTTGG CAGGAGTCCA 
ATGCACAGGG TCACTGTGCA GGAGGTTCTC ACTCTCCTTC CCAATAGCAT GGCTCTGCTG 
CTGCAGAAAG AGCCATGGAA GGAAGAGACC CAGAAGTTCA TTGACTGGCT ATTCAGCATC 
ATGGAAAGCC CTAAAGAAGC CCTCTCAGCA AAGTCCAGGG ATCTTTTGAA AGCCACCCTG 
CTGTCCTTGA GAGTTCTCCC AGAGTTTAAG AAGAAAGCTG TATGGACCAG AGCGTATGGT 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009242667.1
CDS	97..5502
Translation	MSDDIRKRFEFPNSLIQSQAAGHLIAAVLKENGFSEKIYQSTNQTPALNLLWEKCCSDNVVVRTACCEGLVALVAQDHAEFSYVLNGILNLIPSTRNTHGLIKAIMKLLQMQALKEGQGGEKNIQRVYTIRNHPHPLITVLEHRPDCWPVFLQQLTAFFQQCPERSEVSCIQIMAPFLWYLYCEPSQLQEYAKLRLALLKVLLQPQVLCDKDQPSILQQQILQLCCDIVPCLQIKDLIQTTEAMMFIEEVYLSLLRHPVFWKIQLTQMTLQLLCVGEVSLKITGECSSSIHLLEHSVELLKEDFPVELVIIGIALLLLQTPASQQKPILNLALKLFSVTEDQKIPKSSLLLVMPILQILSSTALEDCISVDEEGPSRQQLALNLLEMIQQECYRDDHQKLSYKLVCPVTSMYGSVFTAWRILEVMTDSSAASDWLASVESLLPITTVIPVPAFLLLAHLLVEDKGQNLHQILKVTTELAQADSSQVPNLIPVLMFKLGRPLEPILYNDILYTLPKLGVHKVCIGQILRVIQLLGTTPRLRAVTLRLLTSLWEKQDRVYPELQRFMAVSDVPSLSVGKEVQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQATPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDTRPLILKTLSELFSLVPSLTVNTTEYENFKVQVLSFLWTHTQNKDPIVANAAYRSLANFSAGEHTILHLPEKIRPEIPIPEELDDDEDDEDVDLSVPGSCYLKLLSLTPPLVLPALEEFFTSLVKQEMVNMPRGIYHSALKGGVRSDQGKTVAGIPNFILKMYETNKQPGLKPGLAGGMLFCYDVSMYQSKDGKPLNRLMASRGRSFKQTSLALVHEVHIQLSEWHRAIFLPQAWLAYMNRAYHAILQGRLGELELQLKHGKEEPEEVQYKKSTAWLWVRDMLTDEITKAAAKESPVVKGNALLALSSLAVVVSRHEASLSSDSDGLLEVQPNFLSMKEWVSMILDTLLVIVDSHYQPRGQLLSWFYYKSYSGENTASAIARSAAATALSLLVPVFIISCKEKVEEILNMLTARLPGKPSADESQAVQIHMGLALGMFLSRLCEEKLSDICGQEMNLLLMKSLDALENCCFDTSLEYNTGCILGVGLVLSLMSHSSQMQSCVHVTALLRKLSAYLDDSGSQSRTFQEVLAYTLSCVCTSAFSAGIIEATEAEDVMNKLRLLVENSQQTSGFALALGNVVHGLSVCGHGKAEDLGSKLLPAWIRIVLTEGTPTMLCLAALHGMVALVGSEGDVMQLKSEAIQTSHFQGRLNEVIRTLTQVISVSGVIGLQSNAVWLLGHLHLSTLSSSQSRASVPTDYSYLPESSFIGAAIGFFITGGKKGPESVPPSLLKVVMKPIATVGESYQYPPVNWAALLSPLMRLNFGEEIQQLCLEIMVTQAQSSQNAAALLGLWVTPPLIHSLSLNTKRYLLISAPLWIKHISDEQILGFVENLMVAVFKAASPLGNPELCPSALQGLSQAMKLPSPAHHLWSLLSEATGKIFDLLPNKIRRKDLELYISIAKCLLEMTDDDANRIAQVTKSNIEKAAFVKLYLVSQGRFPLVNLTDMLSVAVQHREKEVLAWMILHSLYQARIVSHANTGVLKRMEWLLELMGYIRNVAYQSTSFQNTALDEALDFFLLIFATAVVAWADHTAPLLLGLSASWLPWHQENGPAGPVPSFLGRSPMHRVTVQEVLTLLPNSMALLLQKEPWKEETQKFIDWLFSIMESPKEALSAKSRDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_009244392.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii focadhesin (FOCAD), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009244392.1

ORF Insert Sequence:

ATGTCAGATG ATATCAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA ATCACAGGCT 
GCGGGTCATC TTATTGCTGC AGTCCTAAAG GAGAATGGTT TTTCAGAAAA GATTTACCAA 
TCTACAAATC AGACTCCTGC TTTGAACTTG CTGTGGGAGA AGTGTTGCAG TGACAATGTA 
GTGGTTCGAA CAGCCTGCTG TGAAGGTCTG GTGGCACTCG TTGCTCAGGA TCATGCAGAG 
TTCAGCTATG TTCTCAATGG GATACTCAAC TTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCATTATGAA ATTACTACAA ATGCAAGCTC TTAAGGAAGG ACAAGGTGGG 
GAAAAGAATA TTCAGAGGGT ATATACCATT AGAAATCATC CTCATCCTTT GATCACTGTG 
CTTGAACACA GACCTGATTG TTGGCCAGTG TTTTTGCAGC AGCTGACAGC ATTTTTCCAG 
CAGTGCCCTG AAAGGTCAGA AGTTTCATGC ATTCAAATAA TGGCACCATT TCTGTGGTAT 
CTGTATTGTG AACCATCTCA GTTACAAGAA TATGCTAAAC TCCGACTAGC CCTGCTGAAA 
GTCTTACTTC AACCCCAGGT TCTTTGTGAC AAGGATCAAC CATCAATACT GCAACAGCAG 
ATACTTCAAC TGTGTTGTGA CATAGTTCCA TGTTTGCAGA TAAAAGATTT GATACAGACA 
ACAGAAGCGA TGATGTTTAT TGAGGAAGTA TATTTAAGCC TTTTGCGTCA TCCTGTTTTC 
TGGAAAATTC AGCTTACCCA GATGACTCTT CAGCTGCTGT GTGTCGGTGA AGTCAGCTTA 
AAGATAACTG GTGAATGTTC ATCTTCAATT CACCTCTTAG AGCACAGTGT TGAACTTCTG 
AAGGAGGATT TTCCTGTTGA ACTGGTCATA ATTGGAATAG CTTTACTACT TCTACAGACT 
CCAGCAAGTC AGCAGAAGCC AATCTTAAAT CTAGCTTTGA AGCTCTTCTC TGTTACTGAG 
GATCAGAAAA TCCCAAAGTC CTCTCTGCTG CTAGTGATGC CAATTCTGCA GATACTGTCT 
TCTACTGCCT TGGAAGACTG TATATCTGTG GATGAAGAAG GTCCCTCTAG GCAGCAGTTG 
GCTCTAAACC TTTTGGAAAT GATACAGCAG GAATGTTACA GAGATGACCA CCAAAAGCTC 
TCCTACAAGC TTGTGTGCCC TGTAACCAGT ATGTATGGTT CAGTATTTAC AGCCTGGAGG 
ATTCTTGAAG TAATGACAGA CTCCTCTGCT GCAAGTGACT GGTTGGCTTC AGTAGAGTCA 
TTGCTTCCTA TTACTACTGT GATCCCTGTG CCTGCCTTTC TTCTGCTGGC TCACCTCCTT 
GTTGAAGACA AAGGACAAAA TCTTCACCAA ATACTCAAGG TCACTACAGA ATTAGCCCAA 
GCAGATTCCT CCCAGGTGCC AAATCTGATT CCAGTTTTGA TGTTCAAATT GGGAAGACCA 
CTGGAACCTA TATTATATAA TGATATATTG TATACTTTAC CTAAGCTTGG TGTTCACAAG 
GTGTGTATAG GACAAATTCT ACGAGTAATA CAACTGCTTG GAACCACACC ACGACTAAGA 
GCTGTCACTT TGCGCTTGCT GACATCTTTG TGGGAAAAGC AGGACCGAGT CTATCCTGAA 
CTGCAGCGTT TCATGGCTGT GTCTGATGTA CCTTCTCTTT CGGTGGGCAA GGAAGTCCAA 
TGGGAGAAAC TGATTGCAAA AGCAGCATCA ATCAGAGATA TATGTAAGCA GAGGCCATAT 
CAACATGGTG CAGATATGCT GGCAGCTATT TCTCAGGTGT TGAATGAATG CACCAAGCCT 
GATCAAGCTA CTCCAGCAGC CTTGGTATTA CAAGGTCTTC ATGCACTCTG TCAAGCTGAG 
GTTGTTTGCA TTCGCTCCAC TTGGAATGCT CTCTCTCCAA AGCTGAGTTG TGACACAAGA 
CCTCTCATTC TGAAGACACT GAGTGAACTA TTTTCTCTAG TTCCTTCCTT AACAGTCAAT 
ACAACTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAC TCATACTCAA 
AACAAGGACC CAATTGTGGC AAATGCTGCA TATAGATCCC TGGCCAATTT TAGTGCAGGA 
GAACACACCA TTCTTCATCT GCCTGAAAAG ATAAGACCAG AAATTCCCAT TCCTGAAGAG 
TTAGATGATG ATGAAGATGA TGAGGATGTG GATCTTTCAG TTCCTGGCTC TTGCTATCTC 
AAACTGTTGT CACTCACTCC CCCTTTGGTT TTACCAGCTT TGGAGGAATT TTTTACATCA 
CTTGTGAAGC AAGAAATGGT GAATATGCCT CGTGGGATAT ATCACTCTGC ATTAAAAGGA 
GGTGTCCGCT CAGACCAAGG AAAGACTGTA GCAGGAATCC CCAATTTTAT ATTGAAAATG 
TATGAAACAA ACAAGCAACC AGGATTGAAA CCTGGCCTTG CAGGTGGTAT GTTATTTTGC 
TATGATGTTT CCATGTATCA GAGTAAAGAT GGAAAACCAT TGAATAGACT GATGGCCAGC 
AGAGGGCGAA GTTTCAAGCA GACATCACTT GCTCTTGTAC ATGAGGTTCA TATCCAGCTT 
TCAGAGTGGC ACCGTGCGAT TTTTCTTCCA CAGGCCTGGC TTGCATACAT GAATCGAGCT 
TATCATGCCA TTTTACAGGG AAGACTAGGA GAGCTGGAGT TGCAGTTAAA ACATGGAAAA 
GAAGAACCTG AGGAGGTGCA GTACAAAAAA AGCACAGCTT GGCTCTGGGT TAGAGACATG 
CTGACTGATG AGATCACCAA GGCAGCTGCA AAGGAGAGTC CGGTAGTGAA AGGCAATGCG 
CTGTTAGCTC TAAGCAGCCT TGCTGTCGTC GTATCTAGAC ATGAAGCCAG CCTCTCCTCA 
GACTCTGACG GGCTCCTGGA GGTTCAACCT AATTTCCTTT CAATGAAAGA GTGGGTTTCC 
ATGATACTTG ATACACTCTT GGTCATTGTG GATAGCCATT ACCAACCCAG AGGGCAACTT 
CTCTCCTGGT TTTATTATAA GTCCTATTCT GGTGAAAACA CAGCTAGTGC CATTGCCCGT 
TCTGCTGCCG CCACGGCTTT GTCTCTCCTT GTGCCAGTTT TCATTATCTC TTGCAAAGAG 
AAGGTTGAGG AAATCCTGAA CATGCTGACT GCCAGGTTAC CTGGGAAACC AAGTGCTGAT 
GAGTCTCAAG CCGTGCAAAT CCACATGGGC CTTGCTTTAG GGATGTTTCT CTCTCGCTTG 
TGTGAAGAGA AACTCAGTGA TATATGTGGC CAAGAGATGA ACCTTCTTCT GATGAAGTCG 
TTGGATGCCC TGGAAAATTG CTGCTTTGAC ACTAGTCTTG AATACAACAC GGGCTGTATA 
TTGGGAGTTG GACTTGTTCT GTCCCTCATG AGTCACAGCA GCCAAATGCA GTCCTGCGTT 
CACGTGACAG CATTGCTTCG GAAGCTGTCT GCATACCTAG ATGACAGTGG GAGCCAGAGC 
AGAACGTTTC AGGAGGTCCT TGCCTACACA CTTAGCTGTG TATGTACATC AGCGTTCAGT 
GCTGGAATTA TTGAGGCTAC AGAGGCTGAG GATGTTATGA ACAAGCTTCG ACTGTTAGTG 
GAGAATAGCC AGCAGACTTC AGGTTTTGCC CTGGCTTTAG GAAACGTAGT TCATGGATTG 
TCTGTGTGTG GACATGGAAA AGCTGAAGAC TTGGGCAGCA AACTACTCCC TGCCTGGATC 
AGAATTGTTC TAACAGAGGG CACTCCCACA ATGCTTTGTC TGGCAGCTCT TCATGGCATG 
GTGGCCCTGG TAGGCTCTGA AGGGGATGTA ATGCAGCTGA AATCAGAAGC CATCCAGACC 
TCTCATTTTC AAGGCAGACT TAATGAAGTC ATTAGAACCT TAACTCAGGT CATTAGTGTC 
TCTGGGGTGA TTGGTCTCCA GTCAAATGCA GTCTGGCTTC TTGGACATCT TCATCTATCT 
ACTCTATCCT CAAGTCAAAG TAGAGCCTCT GTTCCTACTG ACTATAGCTA TTTGCCTGAA 
AGCAGTTTTA TTGGAGCAGC TATTGGCTTC TTCATTACAG GAGGAAAAAA AGGTCCTGAA 
TCTGTGCCTC CTTCCCTTCT TAAAGTAGTG ATGAAACCCA TAGCAACTGT TGGAGAAAGC 
TACCAATATC CTCCTGTGAA CTGGGCTGCA CTTCTCTCTC CACTTATGAG GCTAAATTTT 
GGTGAAGAGA TCCAGCAACT GTGCCTTGAA ATTATGGTGA CCCAGGCACA GTCATCCCAG 
AATGCAGCTG CACTATTGGG CTTGTGGGTG ACACCACCAC TGATCCACAG TCTGAGTCTG 
AATACCAAGA GATATCTCCT GATATCTGCA CCTCTGTGGA TAAAACACAT CTCTGATGAA 
CAGATCCTGG GTTTTGTTGA AAATTTAATG GTGGCAGTTT TTAAAGCAGC TTCCCCACTT 
GGAAATCCTG AGCTATGCCC GAGTGCTTTA CAGGGTCTGA GCCAGGCCAT GAAACTGCCC 
AGCCCTGCTC ACCACCTCTG GAGTCTGCTC TCTGAAGCTA CTGGGAAAAT TTTTGACCTC 
CTGCCAAATA AGATTCGGAG AAAGGATCTA GAGCTGTATA TCAGCATAGC AAAATGCCTC 
TTAGAAATGA CAGATGATGA TGCCAATCGG ATCGCCCAGG TTACTAAGAG CAACATAGAA 
AAGGCTGCCT TTGTCAAACT GTACTTAGTC TCTCAAGGAC GATTCCCCTT GGTGAACCTG 
ACCGATATGC TGAGTGTTGC TGTGCAGCAC CGTGAGAAAG AGGTGTTGGC CTGGATGATT 
CTGCACAGCT TATACCAGGC ACGGATTGTG AGCCATGCCA ACACGGGCGT TTTGAAGAGA 
ATGGAGTGGC TCTTGGAACT GATGGGTTAT ATTAGAAATG TTGCGTACCA GTCAACATCC 
TTTCAGAATA CGGCTCTTGA TGAGGCTTTG GACTTCTTCT TGCTGATATT TGCAACCGCA 
GTGGTTGCAT GGGCCGACCA CACTGCCCCT CTCCTCCTTG GTCTCAGTGC CAGTTGGTTG 
CCATGGCATC AGGAGAATGG CCCGGCTGGG CCAGTACCAA GCTTCCTTGG CAGGAGTCCA 
ATGCACAGGG TCACTGTGCA GGAGGTTCTC ACTCTCCTTC CCAATAGCAT GGCTCTGCTG 
CTGCAGAAAG AGCCATGGAA GGAAGAGACC CAGAAGTTCA TTGACTGGCT ATTCAGCATC 
ATGGAAAGCC CTAAAGAAGC CCTCTCAGCA AAGTCCAGGG ATCTTTTGAA AGCCACCCTG 
CTGTCCTTGA GAGTTCTCCC AGAGTTTAAG AAGAAAGCTG TATGGACCAG AGCGTATGGT 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FOCAD ( XM_009244394.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009242669.1 Pongo abelii focadhesin (FOCAD), transcript variant X6, mRNA.

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CloneID	OSa07887
Clone ID Related Accession (Same CDS sequence)	XM_009244393.1 , XM_009244395.1 , XM_009244392.1 , XM_009244394.1
Accession Version	XM_009244394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-22
Organism	Pongo abelii(Sumatran orangutan)
Product	focadhesin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002883424.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pongo abelii Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGATG ATATCAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA ATCACAGGCT 
GCGGGTCATC TTATTGCTGC AGTCCTAAAG GAGAATGGTT TTTCAGAAAA GATTTACCAA 
TCTACAAATC AGACTCCTGC TTTGAACTTG CTGTGGGAGA AGTGTTGCAG TGACAATGTA 
GTGGTTCGAA CAGCCTGCTG TGAAGGTCTG GTGGCACTCG TTGCTCAGGA TCATGCAGAG 
TTCAGCTATG TTCTCAATGG GATACTCAAC TTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCATTATGAA ATTACTACAA ATGCAAGCTC TTAAGGAAGG ACAAGGTGGG 
GAAAAGAATA TTCAGAGGGT ATATACCATT AGAAATCATC CTCATCCTTT GATCACTGTG 
CTTGAACACA GACCTGATTG TTGGCCAGTG TTTTTGCAGC AGCTGACAGC ATTTTTCCAG 
CAGTGCCCTG AAAGGTCAGA AGTTTCATGC ATTCAAATAA TGGCACCATT TCTGTGGTAT 
CTGTATTGTG AACCATCTCA GTTACAAGAA TATGCTAAAC TCCGACTAGC CCTGCTGAAA 
GTCTTACTTC AACCCCAGGT TCTTTGTGAC AAGGATCAAC CATCAATACT GCAACAGCAG 
ATACTTCAAC TGTGTTGTGA CATAGTTCCA TGTTTGCAGA TAAAAGATTT GATACAGACA 
ACAGAAGCGA TGATGTTTAT TGAGGAAGTA TATTTAAGCC TTTTGCGTCA TCCTGTTTTC 
TGGAAAATTC AGCTTACCCA GATGACTCTT CAGCTGCTGT GTGTCGGTGA AGTCAGCTTA 
AAGATAACTG GTGAATGTTC ATCTTCAATT CACCTCTTAG AGCACAGTGT TGAACTTCTG 
AAGGAGGATT TTCCTGTTGA ACTGGTCATA ATTGGAATAG CTTTACTACT TCTACAGACT 
CCAGCAAGTC AGCAGAAGCC AATCTTAAAT CTAGCTTTGA AGCTCTTCTC TGTTACTGAG 
GATCAGAAAA TCCCAAAGTC CTCTCTGCTG CTAGTGATGC CAATTCTGCA GATACTGTCT 
TCTACTGCCT TGGAAGACTG TATATCTGTG GATGAAGAAG GTCCCTCTAG GCAGCAGTTG 
GCTCTAAACC TTTTGGAAAT GATACAGCAG GAATGTTACA GAGATGACCA CCAAAAGCTC 
TCCTACAAGC TTGTGTGCCC TGTAACCAGT ATGTATGGTT CAGTATTTAC AGCCTGGAGG 
ATTCTTGAAG TAATGACAGA CTCCTCTGCT GCAAGTGACT GGTTGGCTTC AGTAGAGTCA 
TTGCTTCCTA TTACTACTGT GATCCCTGTG CCTGCCTTTC TTCTGCTGGC TCACCTCCTT 
GTTGAAGACA AAGGACAAAA TCTTCACCAA ATACTCAAGG TCACTACAGA ATTAGCCCAA 
GCAGATTCCT CCCAGGTGCC AAATCTGATT CCAGTTTTGA TGTTCAAATT GGGAAGACCA 
CTGGAACCTA TATTATATAA TGATATATTG TATACTTTAC CTAAGCTTGG TGTTCACAAG 
GTGTGTATAG GACAAATTCT ACGAGTAATA CAACTGCTTG GAACCACACC ACGACTAAGA 
GCTGTCACTT TGCGCTTGCT GACATCTTTG TGGGAAAAGC AGGACCGAGT CTATCCTGAA 
CTGCAGCGTT TCATGGCTGT GTCTGATGTA CCTTCTCTTT CGGTGGGCAA GGAAGTCCAA 
TGGGAGAAAC TGATTGCAAA AGCAGCATCA ATCAGAGATA TATGTAAGCA GAGGCCATAT 
CAACATGGTG CAGATATGCT GGCAGCTATT TCTCAGGTGT TGAATGAATG CACCAAGCCT 
GATCAAGCTA CTCCAGCAGC CTTGGTATTA CAAGGTCTTC ATGCACTCTG TCAAGCTGAG 
GTTGTTTGCA TTCGCTCCAC TTGGAATGCT CTCTCTCCAA AGCTGAGTTG TGACACAAGA 
CCTCTCATTC TGAAGACACT GAGTGAACTA TTTTCTCTAG TTCCTTCCTT AACAGTCAAT 
ACAACTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAC TCATACTCAA 
AACAAGGACC CAATTGTGGC AAATGCTGCA TATAGATCCC TGGCCAATTT TAGTGCAGGA 
GAACACACCA TTCTTCATCT GCCTGAAAAG ATAAGACCAG AAATTCCCAT TCCTGAAGAG 
TTAGATGATG ATGAAGATGA TGAGGATGTG GATCTTTCAG TTCCTGGCTC TTGCTATCTC 
AAACTGTTGT CACTCACTCC CCCTTTGGTT TTACCAGCTT TGGAGGAATT TTTTACATCA 
CTTGTGAAGC AAGAAATGGT GAATATGCCT CGTGGGATAT ATCACTCTGC ATTAAAAGGA 
GGTGTCCGCT CAGACCAAGG AAAGACTGTA GCAGGAATCC CCAATTTTAT ATTGAAAATG 
TATGAAACAA ACAAGCAACC AGGATTGAAA CCTGGCCTTG CAGGTGGTAT GTTATTTTGC 
TATGATGTTT CCATGTATCA GAGTAAAGAT GGAAAACCAT TGAATAGACT GATGGCCAGC 
AGAGGGCGAA GTTTCAAGCA GACATCACTT GCTCTTGTAC ATGAGGTTCA TATCCAGCTT 
TCAGAGTGGC ACCGTGCGAT TTTTCTTCCA CAGGCCTGGC TTGCATACAT GAATCGAGCT 
TATCATGCCA TTTTACAGGG AAGACTAGGA GAGCTGGAGT TGCAGTTAAA ACATGGAAAA 
GAAGAACCTG AGGAGGTGCA GTACAAAAAA AGCACAGCTT GGCTCTGGGT TAGAGACATG 
CTGACTGATG AGATCACCAA GGCAGCTGCA AAGGAGAGTC CGGTAGTGAA AGGCAATGCG 
CTGTTAGCTC TAAGCAGCCT TGCTGTCGTC GTATCTAGAC ATGAAGCCAG CCTCTCCTCA 
GACTCTGACG GGCTCCTGGA GGTTCAACCT AATTTCCTTT CAATGAAAGA GTGGGTTTCC 
ATGATACTTG ATACACTCTT GGTCATTGTG GATAGCCATT ACCAACCCAG AGGGCAACTT 
CTCTCCTGGT TTTATTATAA GTCCTATTCT GGTGAAAACA CAGCTAGTGC CATTGCCCGT 
TCTGCTGCCG CCACGGCTTT GTCTCTCCTT GTGCCAGTTT TCATTATCTC TTGCAAAGAG 
AAGGTTGAGG AAATCCTGAA CATGCTGACT GCCAGGTTAC CTGGGAAACC AAGTGCTGAT 
GAGTCTCAAG CCGTGCAAAT CCACATGGGC CTTGCTTTAG GGATGTTTCT CTCTCGCTTG 
TGTGAAGAGA AACTCAGTGA TATATGTGGC CAAGAGATGA ACCTTCTTCT GATGAAGTCG 
TTGGATGCCC TGGAAAATTG CTGCTTTGAC ACTAGTCTTG AATACAACAC GGGCTGTATA 
TTGGGAGTTG GACTTGTTCT GTCCCTCATG AGTCACAGCA GCCAAATGCA GTCCTGCGTT 
CACGTGACAG CATTGCTTCG GAAGCTGTCT GCATACCTAG ATGACAGTGG GAGCCAGAGC 
AGAACGTTTC AGGAGGTCCT TGCCTACACA CTTAGCTGTG TATGTACATC AGCGTTCAGT 
GCTGGAATTA TTGAGGCTAC AGAGGCTGAG GATGTTATGA ACAAGCTTCG ACTGTTAGTG 
GAGAATAGCC AGCAGACTTC AGGTTTTGCC CTGGCTTTAG GAAACGTAGT TCATGGATTG 
TCTGTGTGTG GACATGGAAA AGCTGAAGAC TTGGGCAGCA AACTACTCCC TGCCTGGATC 
AGAATTGTTC TAACAGAGGG CACTCCCACA ATGCTTTGTC TGGCAGCTCT TCATGGCATG 
GTGGCCCTGG TAGGCTCTGA AGGGGATGTA ATGCAGCTGA AATCAGAAGC CATCCAGACC 
TCTCATTTTC AAGGCAGACT TAATGAAGTC ATTAGAACCT TAACTCAGGT CATTAGTGTC 
TCTGGGGTGA TTGGTCTCCA GTCAAATGCA GTCTGGCTTC TTGGACATCT TCATCTATCT 
ACTCTATCCT CAAGTCAAAG TAGAGCCTCT GTTCCTACTG ACTATAGCTA TTTGCCTGAA 
AGCAGTTTTA TTGGAGCAGC TATTGGCTTC TTCATTACAG GAGGAAAAAA AGGTCCTGAA 
TCTGTGCCTC CTTCCCTTCT TAAAGTAGTG ATGAAACCCA TAGCAACTGT TGGAGAAAGC 
TACCAATATC CTCCTGTGAA CTGGGCTGCA CTTCTCTCTC CACTTATGAG GCTAAATTTT 
GGTGAAGAGA TCCAGCAACT GTGCCTTGAA ATTATGGTGA CCCAGGCACA GTCATCCCAG 
AATGCAGCTG CACTATTGGG CTTGTGGGTG ACACCACCAC TGATCCACAG TCTGAGTCTG 
AATACCAAGA GATATCTCCT GATATCTGCA CCTCTGTGGA TAAAACACAT CTCTGATGAA 
CAGATCCTGG GTTTTGTTGA AAATTTAATG GTGGCAGTTT TTAAAGCAGC TTCCCCACTT 
GGAAATCCTG AGCTATGCCC GAGTGCTTTA CAGGGTCTGA GCCAGGCCAT GAAACTGCCC 
AGCCCTGCTC ACCACCTCTG GAGTCTGCTC TCTGAAGCTA CTGGGAAAAT TTTTGACCTC 
CTGCCAAATA AGATTCGGAG AAAGGATCTA GAGCTGTATA TCAGCATAGC AAAATGCCTC 
TTAGAAATGA CAGATGATGA TGCCAATCGG ATCGCCCAGG TTACTAAGAG CAACATAGAA 
AAGGCTGCCT TTGTCAAACT GTACTTAGTC TCTCAAGGAC GATTCCCCTT GGTGAACCTG 
ACCGATATGC TGAGTGTTGC TGTGCAGCAC CGTGAGAAAG AGGTGTTGGC CTGGATGATT 
CTGCACAGCT TATACCAGGC ACGGATTGTG AGCCATGCCA ACACGGGCGT TTTGAAGAGA 
ATGGAGTGGC TCTTGGAACT GATGGGTTAT ATTAGAAATG TTGCGTACCA GTCAACATCC 
TTTCAGAATA CGGCTCTTGA TGAGGCTTTG GACTTCTTCT TGCTGATATT TGCAACCGCA 
GTGGTTGCAT GGGCCGACCA CACTGCCCCT CTCCTCCTTG GTCTCAGTGC CAGTTGGTTG 
CCATGGCATC AGGAGAATGG CCCGGCTGGG CCAGTACCAA GCTTCCTTGG CAGGAGTCCA 
ATGCACAGGG TCACTGTGCA GGAGGTTCTC ACTCTCCTTC CCAATAGCAT GGCTCTGCTG 
CTGCAGAAAG AGCCATGGAA GGAAGAGACC CAGAAGTTCA TTGACTGGCT ATTCAGCATC 
ATGGAAAGCC CTAAAGAAGC CCTCTCAGCA AAGTCCAGGG ATCTTTTGAA AGCCACCCTG 
CTGTCCTTGA GAGTTCTCCC AGAGTTTAAG AAGAAAGCTG TATGGACCAG AGCGTATGGT 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009242669.1
CDS	115..5520
Translation	MSDDIRKRFEFPNSLIQSQAAGHLIAAVLKENGFSEKIYQSTNQTPALNLLWEKCCSDNVVVRTACCEGLVALVAQDHAEFSYVLNGILNLIPSTRNTHGLIKAIMKLLQMQALKEGQGGEKNIQRVYTIRNHPHPLITVLEHRPDCWPVFLQQLTAFFQQCPERSEVSCIQIMAPFLWYLYCEPSQLQEYAKLRLALLKVLLQPQVLCDKDQPSILQQQILQLCCDIVPCLQIKDLIQTTEAMMFIEEVYLSLLRHPVFWKIQLTQMTLQLLCVGEVSLKITGECSSSIHLLEHSVELLKEDFPVELVIIGIALLLLQTPASQQKPILNLALKLFSVTEDQKIPKSSLLLVMPILQILSSTALEDCISVDEEGPSRQQLALNLLEMIQQECYRDDHQKLSYKLVCPVTSMYGSVFTAWRILEVMTDSSAASDWLASVESLLPITTVIPVPAFLLLAHLLVEDKGQNLHQILKVTTELAQADSSQVPNLIPVLMFKLGRPLEPILYNDILYTLPKLGVHKVCIGQILRVIQLLGTTPRLRAVTLRLLTSLWEKQDRVYPELQRFMAVSDVPSLSVGKEVQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQATPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDTRPLILKTLSELFSLVPSLTVNTTEYENFKVQVLSFLWTHTQNKDPIVANAAYRSLANFSAGEHTILHLPEKIRPEIPIPEELDDDEDDEDVDLSVPGSCYLKLLSLTPPLVLPALEEFFTSLVKQEMVNMPRGIYHSALKGGVRSDQGKTVAGIPNFILKMYETNKQPGLKPGLAGGMLFCYDVSMYQSKDGKPLNRLMASRGRSFKQTSLALVHEVHIQLSEWHRAIFLPQAWLAYMNRAYHAILQGRLGELELQLKHGKEEPEEVQYKKSTAWLWVRDMLTDEITKAAAKESPVVKGNALLALSSLAVVVSRHEASLSSDSDGLLEVQPNFLSMKEWVSMILDTLLVIVDSHYQPRGQLLSWFYYKSYSGENTASAIARSAAATALSLLVPVFIISCKEKVEEILNMLTARLPGKPSADESQAVQIHMGLALGMFLSRLCEEKLSDICGQEMNLLLMKSLDALENCCFDTSLEYNTGCILGVGLVLSLMSHSSQMQSCVHVTALLRKLSAYLDDSGSQSRTFQEVLAYTLSCVCTSAFSAGIIEATEAEDVMNKLRLLVENSQQTSGFALALGNVVHGLSVCGHGKAEDLGSKLLPAWIRIVLTEGTPTMLCLAALHGMVALVGSEGDVMQLKSEAIQTSHFQGRLNEVIRTLTQVISVSGVIGLQSNAVWLLGHLHLSTLSSSQSRASVPTDYSYLPESSFIGAAIGFFITGGKKGPESVPPSLLKVVMKPIATVGESYQYPPVNWAALLSPLMRLNFGEEIQQLCLEIMVTQAQSSQNAAALLGLWVTPPLIHSLSLNTKRYLLISAPLWIKHISDEQILGFVENLMVAVFKAASPLGNPELCPSALQGLSQAMKLPSPAHHLWSLLSEATGKIFDLLPNKIRRKDLELYISIAKCLLEMTDDDANRIAQVTKSNIEKAAFVKLYLVSQGRFPLVNLTDMLSVAVQHREKEVLAWMILHSLYQARIVSHANTGVLKRMEWLLELMGYIRNVAYQSTSFQNTALDEALDFFLLIFATAVVAWADHTAPLLLGLSASWLPWHQENGPAGPVPSFLGRSPMHRVTVQEVLTLLPNSMALLLQKEPWKEETQKFIDWLFSIMESPKEALSAKSRDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_009244394.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii focadhesin (FOCAD), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009244394.1

ORF Insert Sequence:

ATGTCAGATG ATATCAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA ATCACAGGCT 
GCGGGTCATC TTATTGCTGC AGTCCTAAAG GAGAATGGTT TTTCAGAAAA GATTTACCAA 
TCTACAAATC AGACTCCTGC TTTGAACTTG CTGTGGGAGA AGTGTTGCAG TGACAATGTA 
GTGGTTCGAA CAGCCTGCTG TGAAGGTCTG GTGGCACTCG TTGCTCAGGA TCATGCAGAG 
TTCAGCTATG TTCTCAATGG GATACTCAAC TTGATTCCAT CAACCAGAAA TACACATGGC 
TTGATAAAAG CCATTATGAA ATTACTACAA ATGCAAGCTC TTAAGGAAGG ACAAGGTGGG 
GAAAAGAATA TTCAGAGGGT ATATACCATT AGAAATCATC CTCATCCTTT GATCACTGTG 
CTTGAACACA GACCTGATTG TTGGCCAGTG TTTTTGCAGC AGCTGACAGC ATTTTTCCAG 
CAGTGCCCTG AAAGGTCAGA AGTTTCATGC ATTCAAATAA TGGCACCATT TCTGTGGTAT 
CTGTATTGTG AACCATCTCA GTTACAAGAA TATGCTAAAC TCCGACTAGC CCTGCTGAAA 
GTCTTACTTC AACCCCAGGT TCTTTGTGAC AAGGATCAAC CATCAATACT GCAACAGCAG 
ATACTTCAAC TGTGTTGTGA CATAGTTCCA TGTTTGCAGA TAAAAGATTT GATACAGACA 
ACAGAAGCGA TGATGTTTAT TGAGGAAGTA TATTTAAGCC TTTTGCGTCA TCCTGTTTTC 
TGGAAAATTC AGCTTACCCA GATGACTCTT CAGCTGCTGT GTGTCGGTGA AGTCAGCTTA 
AAGATAACTG GTGAATGTTC ATCTTCAATT CACCTCTTAG AGCACAGTGT TGAACTTCTG 
AAGGAGGATT TTCCTGTTGA ACTGGTCATA ATTGGAATAG CTTTACTACT TCTACAGACT 
CCAGCAAGTC AGCAGAAGCC AATCTTAAAT CTAGCTTTGA AGCTCTTCTC TGTTACTGAG 
GATCAGAAAA TCCCAAAGTC CTCTCTGCTG CTAGTGATGC CAATTCTGCA GATACTGTCT 
TCTACTGCCT TGGAAGACTG TATATCTGTG GATGAAGAAG GTCCCTCTAG GCAGCAGTTG 
GCTCTAAACC TTTTGGAAAT GATACAGCAG GAATGTTACA GAGATGACCA CCAAAAGCTC 
TCCTACAAGC TTGTGTGCCC TGTAACCAGT ATGTATGGTT CAGTATTTAC AGCCTGGAGG 
ATTCTTGAAG TAATGACAGA CTCCTCTGCT GCAAGTGACT GGTTGGCTTC AGTAGAGTCA 
TTGCTTCCTA TTACTACTGT GATCCCTGTG CCTGCCTTTC TTCTGCTGGC TCACCTCCTT 
GTTGAAGACA AAGGACAAAA TCTTCACCAA ATACTCAAGG TCACTACAGA ATTAGCCCAA 
GCAGATTCCT CCCAGGTGCC AAATCTGATT CCAGTTTTGA TGTTCAAATT GGGAAGACCA 
CTGGAACCTA TATTATATAA TGATATATTG TATACTTTAC CTAAGCTTGG TGTTCACAAG 
GTGTGTATAG GACAAATTCT ACGAGTAATA CAACTGCTTG GAACCACACC ACGACTAAGA 
GCTGTCACTT TGCGCTTGCT GACATCTTTG TGGGAAAAGC AGGACCGAGT CTATCCTGAA 
CTGCAGCGTT TCATGGCTGT GTCTGATGTA CCTTCTCTTT CGGTGGGCAA GGAAGTCCAA 
TGGGAGAAAC TGATTGCAAA AGCAGCATCA ATCAGAGATA TATGTAAGCA GAGGCCATAT 
CAACATGGTG CAGATATGCT GGCAGCTATT TCTCAGGTGT TGAATGAATG CACCAAGCCT 
GATCAAGCTA CTCCAGCAGC CTTGGTATTA CAAGGTCTTC ATGCACTCTG TCAAGCTGAG 
GTTGTTTGCA TTCGCTCCAC TTGGAATGCT CTCTCTCCAA AGCTGAGTTG TGACACAAGA 
CCTCTCATTC TGAAGACACT GAGTGAACTA TTTTCTCTAG TTCCTTCCTT AACAGTCAAT 
ACAACTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAC TCATACTCAA 
AACAAGGACC CAATTGTGGC AAATGCTGCA TATAGATCCC TGGCCAATTT TAGTGCAGGA 
GAACACACCA TTCTTCATCT GCCTGAAAAG ATAAGACCAG AAATTCCCAT TCCTGAAGAG 
TTAGATGATG ATGAAGATGA TGAGGATGTG GATCTTTCAG TTCCTGGCTC TTGCTATCTC 
AAACTGTTGT CACTCACTCC CCCTTTGGTT TTACCAGCTT TGGAGGAATT TTTTACATCA 
CTTGTGAAGC AAGAAATGGT GAATATGCCT CGTGGGATAT ATCACTCTGC ATTAAAAGGA 
GGTGTCCGCT CAGACCAAGG AAAGACTGTA GCAGGAATCC CCAATTTTAT ATTGAAAATG 
TATGAAACAA ACAAGCAACC AGGATTGAAA CCTGGCCTTG CAGGTGGTAT GTTATTTTGC 
TATGATGTTT CCATGTATCA GAGTAAAGAT GGAAAACCAT TGAATAGACT GATGGCCAGC 
AGAGGGCGAA GTTTCAAGCA GACATCACTT GCTCTTGTAC ATGAGGTTCA TATCCAGCTT 
TCAGAGTGGC ACCGTGCGAT TTTTCTTCCA CAGGCCTGGC TTGCATACAT GAATCGAGCT 
TATCATGCCA TTTTACAGGG AAGACTAGGA GAGCTGGAGT TGCAGTTAAA ACATGGAAAA 
GAAGAACCTG AGGAGGTGCA GTACAAAAAA AGCACAGCTT GGCTCTGGGT TAGAGACATG 
CTGACTGATG AGATCACCAA GGCAGCTGCA AAGGAGAGTC CGGTAGTGAA AGGCAATGCG 
CTGTTAGCTC TAAGCAGCCT TGCTGTCGTC GTATCTAGAC ATGAAGCCAG CCTCTCCTCA 
GACTCTGACG GGCTCCTGGA GGTTCAACCT AATTTCCTTT CAATGAAAGA GTGGGTTTCC 
ATGATACTTG ATACACTCTT GGTCATTGTG GATAGCCATT ACCAACCCAG AGGGCAACTT 
CTCTCCTGGT TTTATTATAA GTCCTATTCT GGTGAAAACA CAGCTAGTGC CATTGCCCGT 
TCTGCTGCCG CCACGGCTTT GTCTCTCCTT GTGCCAGTTT TCATTATCTC TTGCAAAGAG 
AAGGTTGAGG AAATCCTGAA CATGCTGACT GCCAGGTTAC CTGGGAAACC AAGTGCTGAT 
GAGTCTCAAG CCGTGCAAAT CCACATGGGC CTTGCTTTAG GGATGTTTCT CTCTCGCTTG 
TGTGAAGAGA AACTCAGTGA TATATGTGGC CAAGAGATGA ACCTTCTTCT GATGAAGTCG 
TTGGATGCCC TGGAAAATTG CTGCTTTGAC ACTAGTCTTG AATACAACAC GGGCTGTATA 
TTGGGAGTTG GACTTGTTCT GTCCCTCATG AGTCACAGCA GCCAAATGCA GTCCTGCGTT 
CACGTGACAG CATTGCTTCG GAAGCTGTCT GCATACCTAG ATGACAGTGG GAGCCAGAGC 
AGAACGTTTC AGGAGGTCCT TGCCTACACA CTTAGCTGTG TATGTACATC AGCGTTCAGT 
GCTGGAATTA TTGAGGCTAC AGAGGCTGAG GATGTTATGA ACAAGCTTCG ACTGTTAGTG 
GAGAATAGCC AGCAGACTTC AGGTTTTGCC CTGGCTTTAG GAAACGTAGT TCATGGATTG 
TCTGTGTGTG GACATGGAAA AGCTGAAGAC TTGGGCAGCA AACTACTCCC TGCCTGGATC 
AGAATTGTTC TAACAGAGGG CACTCCCACA ATGCTTTGTC TGGCAGCTCT TCATGGCATG 
GTGGCCCTGG TAGGCTCTGA AGGGGATGTA ATGCAGCTGA AATCAGAAGC CATCCAGACC 
TCTCATTTTC AAGGCAGACT TAATGAAGTC ATTAGAACCT TAACTCAGGT CATTAGTGTC 
TCTGGGGTGA TTGGTCTCCA GTCAAATGCA GTCTGGCTTC TTGGACATCT TCATCTATCT 
ACTCTATCCT CAAGTCAAAG TAGAGCCTCT GTTCCTACTG ACTATAGCTA TTTGCCTGAA 
AGCAGTTTTA TTGGAGCAGC TATTGGCTTC TTCATTACAG GAGGAAAAAA AGGTCCTGAA 
TCTGTGCCTC CTTCCCTTCT TAAAGTAGTG ATGAAACCCA TAGCAACTGT TGGAGAAAGC 
TACCAATATC CTCCTGTGAA CTGGGCTGCA CTTCTCTCTC CACTTATGAG GCTAAATTTT 
GGTGAAGAGA TCCAGCAACT GTGCCTTGAA ATTATGGTGA CCCAGGCACA GTCATCCCAG 
AATGCAGCTG CACTATTGGG CTTGTGGGTG ACACCACCAC TGATCCACAG TCTGAGTCTG 
AATACCAAGA GATATCTCCT GATATCTGCA CCTCTGTGGA TAAAACACAT CTCTGATGAA 
CAGATCCTGG GTTTTGTTGA AAATTTAATG GTGGCAGTTT TTAAAGCAGC TTCCCCACTT 
GGAAATCCTG AGCTATGCCC GAGTGCTTTA CAGGGTCTGA GCCAGGCCAT GAAACTGCCC 
AGCCCTGCTC ACCACCTCTG GAGTCTGCTC TCTGAAGCTA CTGGGAAAAT TTTTGACCTC 
CTGCCAAATA AGATTCGGAG AAAGGATCTA GAGCTGTATA TCAGCATAGC AAAATGCCTC 
TTAGAAATGA CAGATGATGA TGCCAATCGG ATCGCCCAGG TTACTAAGAG CAACATAGAA 
AAGGCTGCCT TTGTCAAACT GTACTTAGTC TCTCAAGGAC GATTCCCCTT GGTGAACCTG 
ACCGATATGC TGAGTGTTGC TGTGCAGCAC CGTGAGAAAG AGGTGTTGGC CTGGATGATT 
CTGCACAGCT TATACCAGGC ACGGATTGTG AGCCATGCCA ACACGGGCGT TTTGAAGAGA 
ATGGAGTGGC TCTTGGAACT GATGGGTTAT ATTAGAAATG TTGCGTACCA GTCAACATCC 
TTTCAGAATA CGGCTCTTGA TGAGGCTTTG GACTTCTTCT TGCTGATATT TGCAACCGCA 
GTGGTTGCAT GGGCCGACCA CACTGCCCCT CTCCTCCTTG GTCTCAGTGC CAGTTGGTTG 
CCATGGCATC AGGAGAATGG CCCGGCTGGG CCAGTACCAA GCTTCCTTGG CAGGAGTCCA 
ATGCACAGGG TCACTGTGCA GGAGGTTCTC ACTCTCCTTC CCAATAGCAT GGCTCTGCTG 
CTGCAGAAAG AGCCATGGAA GGAAGAGACC CAGAAGTTCA TTGACTGGCT ATTCAGCATC 
ATGGAAAGCC CTAAAGAAGC CCTCTCAGCA AAGTCCAGGG ATCTTTTGAA AGCCACCCTG 
CTGTCCTTGA GAGTTCTCCC AGAGTTTAAG AAGAAAGCTG TATGGACCAG AGCGTATGGT 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FOCAD ( XM_009244389.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009242664.1 Pongo abelii focadhesin (FOCAD), transcript variant X1, mRNA.

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CloneID	OSa28571
Clone ID Related Accession (Same CDS sequence)	XM_009244389.1
Accession Version	XM_009244389.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-22
Organism	Pongo abelii(Sumatran orangutan)
Product	focadhesin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002883424.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pongo abelii Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAATA ATAACTATAT TTGGATGAAG TGGACCAATG AACCATCCAC TGAAGCTCCT 
AAACTGGAGA TTTATGCTTT CGAAGAAGCT GCACACATAC ATGTAAGAGA AGCAAAAATG 
TCAGATGATA TCAGGAAAAG GTTTGAATTT CCAAATTCTC TTATCCAATC ACAGGCTGCG 
GGTCATCTTA TTGCTGCAGT CCTAAAGGAG AATGGTTTTT CAGAAAAGAT TTACCAATCT 
ACAAATCAGA CTCCTGCTTT GAACTTGCTG TGGGAGAAGT GTTGCAGTGA CAATGTAGTG 
GTTCGAACAG CCTGCTGTGA AGGTCTGGTG GCACTCGTTG CTCAGGATCA TGCAGAGTTC 
AGCTATGTTC TCAATGGGAT ACTCAACTTG ATTCCATCAA CCAGAAATAC ACATGGCTTG 
ATAAAAGCCA TTATGAAATT ACTACAAATG CAAGCTCTTA AGGAAGGACA AGGTGGGGAA 
AAGAATATTC AGAGGGTATA TACCATTAGA AATCATCCTC ATCCTTTGAT CACTGTGCTT 
GAACACAGAC CTGATTGTTG GCCAGTGTTT TTGCAGCAGC TGACAGCATT TTTCCAGCAG 
TGCCCTGAAA GGTCAGAAGT TTCATGCATT CAAATAATGG CACCATTTCT GTGGTATCTG 
TATTGTGAAC CATCTCAGTT ACAAGAATAT GCTAAACTCC GACTAGCCCT GCTGAAAGTC 
TTACTTCAAC CCCAGGTTCT TTGTGACAAG GATCAACCAT CAATACTGCA ACAGCAGATA 
CTTCAACTGT GTTGTGACAT AGTTCCATGT TTGCAGATAA AAGATTTGAT ACAGACAACA 
GAAGCGATGA TGTTTATTGA GGAAGTATAT TTAAGCCTTT TGCGTCATCC TGTTTTCTGG 
AAAATTCAGC TTACCCAGAT GACTCTTCAG CTGCTGTGTG TCGGTGAAGT CAGCTTAAAG 
ATAACTGGTG AATGTTCATC TTCAATTCAC CTCTTAGAGC ACAGTGTTGA ACTTCTGAAG 
GAGGATTTTC CTGTTGAACT GGTCATAATT GGAATAGCTT TACTACTTCT ACAGACTCCA 
GCAAGTCAGC AGAAGCCAAT CTTAAATCTA GCTTTGAAGC TCTTCTCTGT TACTGAGGAT 
CAGAAAATCC CAAAGTCCTC TCTGCTGCTA GTGATGCCAA TTCTGCAGAT ACTGTCTTCT 
ACTGCCTTGG AAGACTGTAT ATCTGTGGAT GAAGAAGGTC CCTCTAGGCA GCAGTTGGCT 
CTAAACCTTT TGGAAATGAT ACAGCAGGAA TGTTACAGAG ATGACCACCA AAAGCTCTCC 
TACAAGCTTG TGTGCCCTGT AACCAGTATG TATGGTTCAG TATTTACAGC CTGGAGGATT 
CTTGAAGTAA TGACAGACTC CTCTGCTGCA AGTGACTGGT TGGCTTCAGT AGAGTCATTG 
CTTCCTATTA CTACTGTGAT CCCTGTGCCT GCCTTTCTTC TGCTGGCTCA CCTCCTTGTT 
GAAGACAAAG GACAAAATCT TCACCAAATA CTCAAGGTCA CTACAGAATT AGCCCAAGCA 
GATTCCTCCC AGGTGCCAAA TCTGATTCCA GTTTTGATGT TCAAATTGGG AAGACCACTG 
GAACCTATAT TATATAATGA TATATTGTAT ACTTTACCTA AGCTTGGTGT TCACAAGGTG 
TGTATAGGAC AAATTCTACG AGTAATACAA CTGCTTGGAA CCACACCACG ACTAAGAGCT 
GTCACTTTGC GCTTGCTGAC ATCTTTGTGG GAAAAGCAGG ACCGAGTCTA TCCTGAACTG 
CAGCGTTTCA TGGCTGTGTC TGATGTACCT TCTCTTTCGG TGGGCAAGGA AGTCCAATGG 
GAGAAACTGA TTGCAAAAGC AGCATCAATC AGAGATATAT GTAAGCAGAG GCCATATCAA 
CATGGTGCAG ATATGCTGGC AGCTATTTCT CAGGTGTTGA ATGAATGCAC CAAGCCTGAT 
CAAGCTACTC CAGCAGCCTT GGTATTACAA GGTCTTCATG CACTCTGTCA AGCTGAGGTT 
GTTTGCATTC GCTCCACTTG GAATGCTCTC TCTCCAAAGC TGAGTTGTGA CACAAGACCT 
CTCATTCTGA AGACACTGAG TGAACTATTT TCTCTAGTTC CTTCCTTAAC AGTCAATACA 
ACTGAATATG AGAATTTTAA AGTTCAAGTC CTCAGCTTCC TCTGGACTCA TACTCAAAAC 
AAGGACCCAA TTGTGGCAAA TGCTGCATAT AGATCCCTGG CCAATTTTAG TGCAGGAGAA 
CACACCATTC TTCATCTGCC TGAAAAGATA AGACCAGAAA TTCCCATTCC TGAAGAGTTA 
GATGATGATG AAGATGATGA GGATGTGGAT CTTTCAGTTC CTGGCTCTTG CTATCTCAAA 
CTGTTGTCAC TCACTCCCCC TTTGGTTTTA CCAGCTTTGG AGGAATTTTT TACATCACTT 
GTGAAGCAAG AAATGGTGAA TATGCCTCGT GGGATATATC ACTCTGCATT AAAAGGAGGT 
GTCCGCTCAG ACCAAGGAAA GACTGTAGCA GGAATCCCCA ATTTTATATT GAAAATGTAT 
GAAACAAACA AGCAACCAGG ATTGAAACCT GGCCTTGCAG GTGGTATGTT ATTTTGCTAT 
GATGTTTCCA TGTATCAGAG TAAAGATGGA AAACCATTGA ATAGACTGAT GGCCAGCAGA 
GGGCGAAGTT TCAAGCAGAC ATCACTTGCT CTTGTACATG AGGTTCATAT CCAGCTTTCA 
GAGTGGCACC GTGCGATTTT TCTTCCACAG GCCTGGCTTG CATACATGAA TCGAGCTTAT 
CATGCCATTT TACAGGGAAG ACTAGGAGAG CTGGAGTTGC AGTTAAAACA TGGAAAAGAA 
GAACCTGAGG AGGTGCAGTA CAAAAAAAGC ACAGCTTGGC TCTGGGTTAG AGACATGCTG 
ACTGATGAGA TCACCAAGGC AGCTGCAAAG GAGAGTCCGG TAGTGAAAGG CAATGCGCTG 
TTAGCTCTAA GCAGCCTTGC TGTCGTCGTA TCTAGACATG AAGCCAGCCT CTCCTCAGAC 
TCTGACGGGC TCCTGGAGGT TCAACCTAAT TTCCTTTCAA TGAAAGAGTG GGTTTCCATG 
ATACTTGATA CACTCTTGGT CATTGTGGAT AGCCATTACC AACCCAGAGG GCAACTTCTC 
TCCTGGTTTT ATTATAAGTC CTATTCTGGT GAAAACACAG CTAGTGCCAT TGCCCGTTCT 
GCTGCCGCCA CGGCTTTGTC TCTCCTTGTG CCAGTTTTCA TTATCTCTTG CAAAGAGAAG 
GTTGAGGAAA TCCTGAACAT GCTGACTGCC AGGTTACCTG GGAAACCAAG TGCTGATGAG 
TCTCAAGCCG TGCAAATCCA CATGGGCCTT GCTTTAGGGA TGTTTCTCTC TCGCTTGTGT 
GAAGAGAAAC TCAGTGATAT ATGTGGCCAA GAGATGAACC TTCTTCTGAT GAAGTCGTTG 
GATGCCCTGG AAAATTGCTG CTTTGACACT AGTCTTGAAT ACAACACGGG CTGTATATTG 
GGAGTTGGAC TTGTTCTGTC CCTCATGAGT CACAGCAGCC AAATGCAGTC CTGCGTTCAC 
GTGACAGCAT TGCTTCGGAA GCTGTCTGCA TACCTAGATG ACAGTGGGAG CCAGAGCAGA 
ACGTTTCAGG AGGTCCTTGC CTACACACTT AGCTGTGTAT GTACATCAGC GTTCAGTGCT 
GGAATTATTG AGGCTACAGA GGCTGAGGAT GTTATGAACA AGCTTCGACT GTTAGTGGAG 
AATAGCCAGC AGACTTCAGG TTTTGCCCTG GCTTTAGGAA ACGTAGTTCA TGGATTGTCT 
GTGTGTGGAC ATGGAAAAGC TGAAGACTTG GGCAGCAAAC TACTCCCTGC CTGGATCAGA 
ATTGTTCTAA CAGAGGGCAC TCCCACAATG CTTTGTCTGG CAGCTCTTCA TGGCATGGTG 
GCCCTGGTAG GCTCTGAAGG GGATGTAATG CAGCTGAAAT CAGAAGCCAT CCAGACCTCT 
CATTTTCAAG GCAGACTTAA TGAAGTCATT AGAACCTTAA CTCAGGTCAT TAGTGTCTCT 
GGGGTGATTG GTCTCCAGTC AAATGCAGTC TGGCTTCTTG GACATCTTCA TCTATCTACT 
CTATCCTCAA GTCAAAGTAG AGCCTCTGTT CCTACTGACT ATAGCTATTT GCCTGAAAGC 
AGTTTTATTG GAGCAGCTAT TGGCTTCTTC ATTACAGGAG GAAAAAAAGG TCCTGAATCT 
GTGCCTCCTT CCCTTCTTAA AGTAGTGATG AAACCCATAG CAACTGTTGG AGAAAGCTAC 
CAATATCCTC CTGTGAACTG GGCTGCACTT CTCTCTCCAC TTATGAGGCT AAATTTTGGT 
GAAGAGATCC AGCAACTGTG CCTTGAAATT ATGGTGACCC AGGCACAGTC ATCCCAGAAT 
GCAGCTGCAC TATTGGGCTT GTGGGTGACA CCACCACTGA TCCACAGTCT GAGTCTGAAT 
ACCAAGAGAT ATCTCCTGAT ATCTGCACCT CTGTGGATAA AACACATCTC TGATGAACAG 
ATCCTGGGTT TTGTTGAAAA TTTAATGGTG GCAGTTTTTA AAGCAGCTTC CCCACTTGGA 
AATCCTGAGC TATGCCCGAG TGCTTTACAG GGTCTGAGCC AGGCCATGAA ACTGCCCAGC 
CCTGCTCACC ACCTCTGGAG TCTGCTCTCT GAAGCTACTG GGAAAATTTT TGACCTCCTG 
CCAAATAAGA TTCGGAGAAA GGATCTAGAG CTGTATATCA GCATAGCAAA ATGCCTCTTA 
GAAATGACAG ATGATGATGC CAATCGGATC GCCCAGGTTA CTAAGAGCAA CATAGAAAAG 
GCTGCCTTTG TCAAACTGTA CTTAGTCTCT CAAGGACGAT TCCCCTTGGT GAACCTGACC 
GATATGCTGA GTGTTGCTGT GCAGCACCGT GAGAAAGAGG TGTTGGCCTG GATGATTCTG 
CACAGCTTAT ACCAGGCACG GATTGTGAGC CATGCCAACA CGGGCGTTTT GAAGAGAATG 
GAGTGGCTCT TGGAACTGAT GGGTTATATT AGAAATGTTG CGTACCAGTC AACATCCTTT 
CAGAATACGG CTCTTGATGA GGCTTTGGAC TTCTTCTTGC TGATATTTGC AACCGCAGTG 
GTTGCATGGG CCGACCACAC TGCCCCTCTC CTCCTTGGTC TCAGTGCCAG TTGGTTGCCA 
TGGCATCAGG AGAATGGCCC GGCTGGGCCA GTACCAAGCT TCCTTGGCAG GAGTCCAATG 
CACAGGGTCA CTGTGCAGGA GGTTCTCACT CTCCTTCCCA ATAGCATGGC TCTGCTGCTG 
CAGAAAGAGC CATGGAAGGA AGAGACCCAG AAGTTCATTG ACTGGCTATT CAGCATCATG 
GAAAGCCCTA AAGAAGCCCT CTCAGCAAAG TCCAGGGATC TTTTGAAAGC CACCCTGCTG 
TCCTTGAGAG TTCTCCCAGA GTTTAAGAAG AAAGCTGTAT GGACCAGAGC GTATGGTTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009242664.1
CDS	66..5588
Translation	MKNNNYIWMKWTNEPSTEAPKLEIYAFEEAAHIHVREAKMSDDIRKRFEFPNSLIQSQAAGHLIAAVLKENGFSEKIYQSTNQTPALNLLWEKCCSDNVVVRTACCEGLVALVAQDHAEFSYVLNGILNLIPSTRNTHGLIKAIMKLLQMQALKEGQGGEKNIQRVYTIRNHPHPLITVLEHRPDCWPVFLQQLTAFFQQCPERSEVSCIQIMAPFLWYLYCEPSQLQEYAKLRLALLKVLLQPQVLCDKDQPSILQQQILQLCCDIVPCLQIKDLIQTTEAMMFIEEVYLSLLRHPVFWKIQLTQMTLQLLCVGEVSLKITGECSSSIHLLEHSVELLKEDFPVELVIIGIALLLLQTPASQQKPILNLALKLFSVTEDQKIPKSSLLLVMPILQILSSTALEDCISVDEEGPSRQQLALNLLEMIQQECYRDDHQKLSYKLVCPVTSMYGSVFTAWRILEVMTDSSAASDWLASVESLLPITTVIPVPAFLLLAHLLVEDKGQNLHQILKVTTELAQADSSQVPNLIPVLMFKLGRPLEPILYNDILYTLPKLGVHKVCIGQILRVIQLLGTTPRLRAVTLRLLTSLWEKQDRVYPELQRFMAVSDVPSLSVGKEVQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQATPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDTRPLILKTLSELFSLVPSLTVNTTEYENFKVQVLSFLWTHTQNKDPIVANAAYRSLANFSAGEHTILHLPEKIRPEIPIPEELDDDEDDEDVDLSVPGSCYLKLLSLTPPLVLPALEEFFTSLVKQEMVNMPRGIYHSALKGGVRSDQGKTVAGIPNFILKMYETNKQPGLKPGLAGGMLFCYDVSMYQSKDGKPLNRLMASRGRSFKQTSLALVHEVHIQLSEWHRAIFLPQAWLAYMNRAYHAILQGRLGELELQLKHGKEEPEEVQYKKSTAWLWVRDMLTDEITKAAAKESPVVKGNALLALSSLAVVVSRHEASLSSDSDGLLEVQPNFLSMKEWVSMILDTLLVIVDSHYQPRGQLLSWFYYKSYSGENTASAIARSAAATALSLLVPVFIISCKEKVEEILNMLTARLPGKPSADESQAVQIHMGLALGMFLSRLCEEKLSDICGQEMNLLLMKSLDALENCCFDTSLEYNTGCILGVGLVLSLMSHSSQMQSCVHVTALLRKLSAYLDDSGSQSRTFQEVLAYTLSCVCTSAFSAGIIEATEAEDVMNKLRLLVENSQQTSGFALALGNVVHGLSVCGHGKAEDLGSKLLPAWIRIVLTEGTPTMLCLAALHGMVALVGSEGDVMQLKSEAIQTSHFQGRLNEVIRTLTQVISVSGVIGLQSNAVWLLGHLHLSTLSSSQSRASVPTDYSYLPESSFIGAAIGFFITGGKKGPESVPPSLLKVVMKPIATVGESYQYPPVNWAALLSPLMRLNFGEEIQQLCLEIMVTQAQSSQNAAALLGLWVTPPLIHSLSLNTKRYLLISAPLWIKHISDEQILGFVENLMVAVFKAASPLGNPELCPSALQGLSQAMKLPSPAHHLWSLLSEATGKIFDLLPNKIRRKDLELYISIAKCLLEMTDDDANRIAQVTKSNIEKAAFVKLYLVSQGRFPLVNLTDMLSVAVQHREKEVLAWMILHSLYQARIVSHANTGVLKRMEWLLELMGYIRNVAYQSTSFQNTALDEALDFFLLIFATAVVAWADHTAPLLLGLSASWLPWHQENGPAGPVPSFLGRSPMHRVTVQEVLTLLPNSMALLLQKEPWKEETQKFIDWLFSIMESPKEALSAKSRDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_009244389.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii focadhesin (FOCAD), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009244389.1

ORF Insert Sequence:

ATGAAAAATA ATAACTATAT TTGGATGAAG TGGACCAATG AACCATCCAC TGAAGCTCCT 
AAACTGGAGA TTTATGCTTT CGAAGAAGCT GCACACATAC ATGTAAGAGA AGCAAAAATG 
TCAGATGATA TCAGGAAAAG GTTTGAATTT CCAAATTCTC TTATCCAATC ACAGGCTGCG 
GGTCATCTTA TTGCTGCAGT CCTAAAGGAG AATGGTTTTT CAGAAAAGAT TTACCAATCT 
ACAAATCAGA CTCCTGCTTT GAACTTGCTG TGGGAGAAGT GTTGCAGTGA CAATGTAGTG 
GTTCGAACAG CCTGCTGTGA AGGTCTGGTG GCACTCGTTG CTCAGGATCA TGCAGAGTTC 
AGCTATGTTC TCAATGGGAT ACTCAACTTG ATTCCATCAA CCAGAAATAC ACATGGCTTG 
ATAAAAGCCA TTATGAAATT ACTACAAATG CAAGCTCTTA AGGAAGGACA AGGTGGGGAA 
AAGAATATTC AGAGGGTATA TACCATTAGA AATCATCCTC ATCCTTTGAT CACTGTGCTT 
GAACACAGAC CTGATTGTTG GCCAGTGTTT TTGCAGCAGC TGACAGCATT TTTCCAGCAG 
TGCCCTGAAA GGTCAGAAGT TTCATGCATT CAAATAATGG CACCATTTCT GTGGTATCTG 
TATTGTGAAC CATCTCAGTT ACAAGAATAT GCTAAACTCC GACTAGCCCT GCTGAAAGTC 
TTACTTCAAC CCCAGGTTCT TTGTGACAAG GATCAACCAT CAATACTGCA ACAGCAGATA 
CTTCAACTGT GTTGTGACAT AGTTCCATGT TTGCAGATAA AAGATTTGAT ACAGACAACA 
GAAGCGATGA TGTTTATTGA GGAAGTATAT TTAAGCCTTT TGCGTCATCC TGTTTTCTGG 
AAAATTCAGC TTACCCAGAT GACTCTTCAG CTGCTGTGTG TCGGTGAAGT CAGCTTAAAG 
ATAACTGGTG AATGTTCATC TTCAATTCAC CTCTTAGAGC ACAGTGTTGA ACTTCTGAAG 
GAGGATTTTC CTGTTGAACT GGTCATAATT GGAATAGCTT TACTACTTCT ACAGACTCCA 
GCAAGTCAGC AGAAGCCAAT CTTAAATCTA GCTTTGAAGC TCTTCTCTGT TACTGAGGAT 
CAGAAAATCC CAAAGTCCTC TCTGCTGCTA GTGATGCCAA TTCTGCAGAT ACTGTCTTCT 
ACTGCCTTGG AAGACTGTAT ATCTGTGGAT GAAGAAGGTC CCTCTAGGCA GCAGTTGGCT 
CTAAACCTTT TGGAAATGAT ACAGCAGGAA TGTTACAGAG ATGACCACCA AAAGCTCTCC 
TACAAGCTTG TGTGCCCTGT AACCAGTATG TATGGTTCAG TATTTACAGC CTGGAGGATT 
CTTGAAGTAA TGACAGACTC CTCTGCTGCA AGTGACTGGT TGGCTTCAGT AGAGTCATTG 
CTTCCTATTA CTACTGTGAT CCCTGTGCCT GCCTTTCTTC TGCTGGCTCA CCTCCTTGTT 
GAAGACAAAG GACAAAATCT TCACCAAATA CTCAAGGTCA CTACAGAATT AGCCCAAGCA 
GATTCCTCCC AGGTGCCAAA TCTGATTCCA GTTTTGATGT TCAAATTGGG AAGACCACTG 
GAACCTATAT TATATAATGA TATATTGTAT ACTTTACCTA AGCTTGGTGT TCACAAGGTG 
TGTATAGGAC AAATTCTACG AGTAATACAA CTGCTTGGAA CCACACCACG ACTAAGAGCT 
GTCACTTTGC GCTTGCTGAC ATCTTTGTGG GAAAAGCAGG ACCGAGTCTA TCCTGAACTG 
CAGCGTTTCA TGGCTGTGTC TGATGTACCT TCTCTTTCGG TGGGCAAGGA AGTCCAATGG 
GAGAAACTGA TTGCAAAAGC AGCATCAATC AGAGATATAT GTAAGCAGAG GCCATATCAA 
CATGGTGCAG ATATGCTGGC AGCTATTTCT CAGGTGTTGA ATGAATGCAC CAAGCCTGAT 
CAAGCTACTC CAGCAGCCTT GGTATTACAA GGTCTTCATG CACTCTGTCA AGCTGAGGTT 
GTTTGCATTC GCTCCACTTG GAATGCTCTC TCTCCAAAGC TGAGTTGTGA CACAAGACCT 
CTCATTCTGA AGACACTGAG TGAACTATTT TCTCTAGTTC CTTCCTTAAC AGTCAATACA 
ACTGAATATG AGAATTTTAA AGTTCAAGTC CTCAGCTTCC TCTGGACTCA TACTCAAAAC 
AAGGACCCAA TTGTGGCAAA TGCTGCATAT AGATCCCTGG CCAATTTTAG TGCAGGAGAA 
CACACCATTC TTCATCTGCC TGAAAAGATA AGACCAGAAA TTCCCATTCC TGAAGAGTTA 
GATGATGATG AAGATGATGA GGATGTGGAT CTTTCAGTTC CTGGCTCTTG CTATCTCAAA 
CTGTTGTCAC TCACTCCCCC TTTGGTTTTA CCAGCTTTGG AGGAATTTTT TACATCACTT 
GTGAAGCAAG AAATGGTGAA TATGCCTCGT GGGATATATC ACTCTGCATT AAAAGGAGGT 
GTCCGCTCAG ACCAAGGAAA GACTGTAGCA GGAATCCCCA ATTTTATATT GAAAATGTAT 
GAAACAAACA AGCAACCAGG ATTGAAACCT GGCCTTGCAG GTGGTATGTT ATTTTGCTAT 
GATGTTTCCA TGTATCAGAG TAAAGATGGA AAACCATTGA ATAGACTGAT GGCCAGCAGA 
GGGCGAAGTT TCAAGCAGAC ATCACTTGCT CTTGTACATG AGGTTCATAT CCAGCTTTCA 
GAGTGGCACC GTGCGATTTT TCTTCCACAG GCCTGGCTTG CATACATGAA TCGAGCTTAT 
CATGCCATTT TACAGGGAAG ACTAGGAGAG CTGGAGTTGC AGTTAAAACA TGGAAAAGAA 
GAACCTGAGG AGGTGCAGTA CAAAAAAAGC ACAGCTTGGC TCTGGGTTAG AGACATGCTG 
ACTGATGAGA TCACCAAGGC AGCTGCAAAG GAGAGTCCGG TAGTGAAAGG CAATGCGCTG 
TTAGCTCTAA GCAGCCTTGC TGTCGTCGTA TCTAGACATG AAGCCAGCCT CTCCTCAGAC 
TCTGACGGGC TCCTGGAGGT TCAACCTAAT TTCCTTTCAA TGAAAGAGTG GGTTTCCATG 
ATACTTGATA CACTCTTGGT CATTGTGGAT AGCCATTACC AACCCAGAGG GCAACTTCTC 
TCCTGGTTTT ATTATAAGTC CTATTCTGGT GAAAACACAG CTAGTGCCAT TGCCCGTTCT 
GCTGCCGCCA CGGCTTTGTC TCTCCTTGTG CCAGTTTTCA TTATCTCTTG CAAAGAGAAG 
GTTGAGGAAA TCCTGAACAT GCTGACTGCC AGGTTACCTG GGAAACCAAG TGCTGATGAG 
TCTCAAGCCG TGCAAATCCA CATGGGCCTT GCTTTAGGGA TGTTTCTCTC TCGCTTGTGT 
GAAGAGAAAC TCAGTGATAT ATGTGGCCAA GAGATGAACC TTCTTCTGAT GAAGTCGTTG 
GATGCCCTGG AAAATTGCTG CTTTGACACT AGTCTTGAAT ACAACACGGG CTGTATATTG 
GGAGTTGGAC TTGTTCTGTC CCTCATGAGT CACAGCAGCC AAATGCAGTC CTGCGTTCAC 
GTGACAGCAT TGCTTCGGAA GCTGTCTGCA TACCTAGATG ACAGTGGGAG CCAGAGCAGA 
ACGTTTCAGG AGGTCCTTGC CTACACACTT AGCTGTGTAT GTACATCAGC GTTCAGTGCT 
GGAATTATTG AGGCTACAGA GGCTGAGGAT GTTATGAACA AGCTTCGACT GTTAGTGGAG 
AATAGCCAGC AGACTTCAGG TTTTGCCCTG GCTTTAGGAA ACGTAGTTCA TGGATTGTCT 
GTGTGTGGAC ATGGAAAAGC TGAAGACTTG GGCAGCAAAC TACTCCCTGC CTGGATCAGA 
ATTGTTCTAA CAGAGGGCAC TCCCACAATG CTTTGTCTGG CAGCTCTTCA TGGCATGGTG 
GCCCTGGTAG GCTCTGAAGG GGATGTAATG CAGCTGAAAT CAGAAGCCAT CCAGACCTCT 
CATTTTCAAG GCAGACTTAA TGAAGTCATT AGAACCTTAA CTCAGGTCAT TAGTGTCTCT 
GGGGTGATTG GTCTCCAGTC AAATGCAGTC TGGCTTCTTG GACATCTTCA TCTATCTACT 
CTATCCTCAA GTCAAAGTAG AGCCTCTGTT CCTACTGACT ATAGCTATTT GCCTGAAAGC 
AGTTTTATTG GAGCAGCTAT TGGCTTCTTC ATTACAGGAG GAAAAAAAGG TCCTGAATCT 
GTGCCTCCTT CCCTTCTTAA AGTAGTGATG AAACCCATAG CAACTGTTGG AGAAAGCTAC 
CAATATCCTC CTGTGAACTG GGCTGCACTT CTCTCTCCAC TTATGAGGCT AAATTTTGGT 
GAAGAGATCC AGCAACTGTG CCTTGAAATT ATGGTGACCC AGGCACAGTC ATCCCAGAAT 
GCAGCTGCAC TATTGGGCTT GTGGGTGACA CCACCACTGA TCCACAGTCT GAGTCTGAAT 
ACCAAGAGAT ATCTCCTGAT ATCTGCACCT CTGTGGATAA AACACATCTC TGATGAACAG 
ATCCTGGGTT TTGTTGAAAA TTTAATGGTG GCAGTTTTTA AAGCAGCTTC CCCACTTGGA 
AATCCTGAGC TATGCCCGAG TGCTTTACAG GGTCTGAGCC AGGCCATGAA ACTGCCCAGC 
CCTGCTCACC ACCTCTGGAG TCTGCTCTCT GAAGCTACTG GGAAAATTTT TGACCTCCTG 
CCAAATAAGA TTCGGAGAAA GGATCTAGAG CTGTATATCA GCATAGCAAA ATGCCTCTTA 
GAAATGACAG ATGATGATGC CAATCGGATC GCCCAGGTTA CTAAGAGCAA CATAGAAAAG 
GCTGCCTTTG TCAAACTGTA CTTAGTCTCT CAAGGACGAT TCCCCTTGGT GAACCTGACC 
GATATGCTGA GTGTTGCTGT GCAGCACCGT GAGAAAGAGG TGTTGGCCTG GATGATTCTG 
CACAGCTTAT ACCAGGCACG GATTGTGAGC CATGCCAACA CGGGCGTTTT GAAGAGAATG 
GAGTGGCTCT TGGAACTGAT GGGTTATATT AGAAATGTTG CGTACCAGTC AACATCCTTT 
CAGAATACGG CTCTTGATGA GGCTTTGGAC TTCTTCTTGC TGATATTTGC AACCGCAGTG 
GTTGCATGGG CCGACCACAC TGCCCCTCTC CTCCTTGGTC TCAGTGCCAG TTGGTTGCCA 
TGGCATCAGG AGAATGGCCC GGCTGGGCCA GTACCAAGCT TCCTTGGCAG GAGTCCAATG 
CACAGGGTCA CTGTGCAGGA GGTTCTCACT CTCCTTCCCA ATAGCATGGC TCTGCTGCTG 
CAGAAAGAGC CATGGAAGGA AGAGACCCAG AAGTTCATTG ACTGGCTATT CAGCATCATG 
GAAAGCCCTA AAGAAGCCCT CTCAGCAAAG TCCAGGGATC TTTTGAAAGC CACCCTGCTG 
TCCTTGAGAG TTCTCCCAGA GTTTAAGAAG AAAGCTGTAT GGACCAGAGC GTATGGTTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FOCAD ( XM_009244390.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009242665.1 Pongo abelii focadhesin (FOCAD), transcript variant X2, mRNA.

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CloneID	OSa28572
Clone ID Related Accession (Same CDS sequence)	XM_009244390.1
Accession Version	XM_009244390.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-22
Organism	Pongo abelii(Sumatran orangutan)
Product	focadhesin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002883424.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pongo abelii Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAACA AAGGAAGGCA AGAAGCTGCA CACATACATG TAAGAGAAGC AAAAATGTCA 
GATGATATCA GGAAAAGGTT TGAATTTCCA AATTCTCTTA TCCAATCACA GGCTGCGGGT 
CATCTTATTG CTGCAGTCCT AAAGGAGAAT GGTTTTTCAG AAAAGATTTA CCAATCTACA 
AATCAGACTC CTGCTTTGAA CTTGCTGTGG GAGAAGTGTT GCAGTGACAA TGTAGTGGTT 
CGAACAGCCT GCTGTGAAGG TCTGGTGGCA CTCGTTGCTC AGGATCATGC AGAGTTCAGC 
TATGTTCTCA ATGGGATACT CAACTTGATT CCATCAACCA GAAATACACA TGGCTTGATA 
AAAGCCATTA TGAAATTACT ACAAATGCAA GCTCTTAAGG AAGGACAAGG TGGGGAAAAG 
AATATTCAGA GGGTATATAC CATTAGAAAT CATCCTCATC CTTTGATCAC TGTGCTTGAA 
CACAGACCTG ATTGTTGGCC AGTGTTTTTG CAGCAGCTGA CAGCATTTTT CCAGCAGTGC 
CCTGAAAGGT CAGAAGTTTC ATGCATTCAA ATAATGGCAC CATTTCTGTG GTATCTGTAT 
TGTGAACCAT CTCAGTTACA AGAATATGCT AAACTCCGAC TAGCCCTGCT GAAAGTCTTA 
CTTCAACCCC AGGTTCTTTG TGACAAGGAT CAACCATCAA TACTGCAACA GCAGATACTT 
CAACTGTGTT GTGACATAGT TCCATGTTTG CAGATAAAAG ATTTGATACA GACAACAGAA 
GCGATGATGT TTATTGAGGA AGTATATTTA AGCCTTTTGC GTCATCCTGT TTTCTGGAAA 
ATTCAGCTTA CCCAGATGAC TCTTCAGCTG CTGTGTGTCG GTGAAGTCAG CTTAAAGATA 
ACTGGTGAAT GTTCATCTTC AATTCACCTC TTAGAGCACA GTGTTGAACT TCTGAAGGAG 
GATTTTCCTG TTGAACTGGT CATAATTGGA ATAGCTTTAC TACTTCTACA GACTCCAGCA 
AGTCAGCAGA AGCCAATCTT AAATCTAGCT TTGAAGCTCT TCTCTGTTAC TGAGGATCAG 
AAAATCCCAA AGTCCTCTCT GCTGCTAGTG ATGCCAATTC TGCAGATACT GTCTTCTACT 
GCCTTGGAAG ACTGTATATC TGTGGATGAA GAAGGTCCCT CTAGGCAGCA GTTGGCTCTA 
AACCTTTTGG AAATGATACA GCAGGAATGT TACAGAGATG ACCACCAAAA GCTCTCCTAC 
AAGCTTGTGT GCCCTGTAAC CAGTATGTAT GGTTCAGTAT TTACAGCCTG GAGGATTCTT 
GAAGTAATGA CAGACTCCTC TGCTGCAAGT GACTGGTTGG CTTCAGTAGA GTCATTGCTT 
CCTATTACTA CTGTGATCCC TGTGCCTGCC TTTCTTCTGC TGGCTCACCT CCTTGTTGAA 
GACAAAGGAC AAAATCTTCA CCAAATACTC AAGGTCACTA CAGAATTAGC CCAAGCAGAT 
TCCTCCCAGG TGCCAAATCT GATTCCAGTT TTGATGTTCA AATTGGGAAG ACCACTGGAA 
CCTATATTAT ATAATGATAT ATTGTATACT TTACCTAAGC TTGGTGTTCA CAAGGTGTGT 
ATAGGACAAA TTCTACGAGT AATACAACTG CTTGGAACCA CACCACGACT AAGAGCTGTC 
ACTTTGCGCT TGCTGACATC TTTGTGGGAA AAGCAGGACC GAGTCTATCC TGAACTGCAG 
CGTTTCATGG CTGTGTCTGA TGTACCTTCT CTTTCGGTGG GCAAGGAAGT CCAATGGGAG 
AAACTGATTG CAAAAGCAGC ATCAATCAGA GATATATGTA AGCAGAGGCC ATATCAACAT 
GGTGCAGATA TGCTGGCAGC TATTTCTCAG GTGTTGAATG AATGCACCAA GCCTGATCAA 
GCTACTCCAG CAGCCTTGGT ATTACAAGGT CTTCATGCAC TCTGTCAAGC TGAGGTTGTT 
TGCATTCGCT CCACTTGGAA TGCTCTCTCT CCAAAGCTGA GTTGTGACAC AAGACCTCTC 
ATTCTGAAGA CACTGAGTGA ACTATTTTCT CTAGTTCCTT CCTTAACAGT CAATACAACT 
GAATATGAGA ATTTTAAAGT TCAAGTCCTC AGCTTCCTCT GGACTCATAC TCAAAACAAG 
GACCCAATTG TGGCAAATGC TGCATATAGA TCCCTGGCCA ATTTTAGTGC AGGAGAACAC 
ACCATTCTTC ATCTGCCTGA AAAGATAAGA CCAGAAATTC CCATTCCTGA AGAGTTAGAT 
GATGATGAAG ATGATGAGGA TGTGGATCTT TCAGTTCCTG GCTCTTGCTA TCTCAAACTG 
TTGTCACTCA CTCCCCCTTT GGTTTTACCA GCTTTGGAGG AATTTTTTAC ATCACTTGTG 
AAGCAAGAAA TGGTGAATAT GCCTCGTGGG ATATATCACT CTGCATTAAA AGGAGGTGTC 
CGCTCAGACC AAGGAAAGAC TGTAGCAGGA ATCCCCAATT TTATATTGAA AATGTATGAA 
ACAAACAAGC AACCAGGATT GAAACCTGGC CTTGCAGGTG GTATGTTATT TTGCTATGAT 
GTTTCCATGT ATCAGAGTAA AGATGGAAAA CCATTGAATA GACTGATGGC CAGCAGAGGG 
CGAAGTTTCA AGCAGACATC ACTTGCTCTT GTACATGAGG TTCATATCCA GCTTTCAGAG 
TGGCACCGTG CGATTTTTCT TCCACAGGCC TGGCTTGCAT ACATGAATCG AGCTTATCAT 
GCCATTTTAC AGGGAAGACT AGGAGAGCTG GAGTTGCAGT TAAAACATGG AAAAGAAGAA 
CCTGAGGAGG TGCAGTACAA AAAAAGCACA GCTTGGCTCT GGGTTAGAGA CATGCTGACT 
GATGAGATCA CCAAGGCAGC TGCAAAGGAG AGTCCGGTAG TGAAAGGCAA TGCGCTGTTA 
GCTCTAAGCA GCCTTGCTGT CGTCGTATCT AGACATGAAG CCAGCCTCTC CTCAGACTCT 
GACGGGCTCC TGGAGGTTCA ACCTAATTTC CTTTCAATGA AAGAGTGGGT TTCCATGATA 
CTTGATACAC TCTTGGTCAT TGTGGATAGC CATTACCAAC CCAGAGGGCA ACTTCTCTCC 
TGGTTTTATT ATAAGTCCTA TTCTGGTGAA AACACAGCTA GTGCCATTGC CCGTTCTGCT 
GCCGCCACGG CTTTGTCTCT CCTTGTGCCA GTTTTCATTA TCTCTTGCAA AGAGAAGGTT 
GAGGAAATCC TGAACATGCT GACTGCCAGG TTACCTGGGA AACCAAGTGC TGATGAGTCT 
CAAGCCGTGC AAATCCACAT GGGCCTTGCT TTAGGGATGT TTCTCTCTCG CTTGTGTGAA 
GAGAAACTCA GTGATATATG TGGCCAAGAG ATGAACCTTC TTCTGATGAA GTCGTTGGAT 
GCCCTGGAAA ATTGCTGCTT TGACACTAGT CTTGAATACA ACACGGGCTG TATATTGGGA 
GTTGGACTTG TTCTGTCCCT CATGAGTCAC AGCAGCCAAA TGCAGTCCTG CGTTCACGTG 
ACAGCATTGC TTCGGAAGCT GTCTGCATAC CTAGATGACA GTGGGAGCCA GAGCAGAACG 
TTTCAGGAGG TCCTTGCCTA CACACTTAGC TGTGTATGTA CATCAGCGTT CAGTGCTGGA 
ATTATTGAGG CTACAGAGGC TGAGGATGTT ATGAACAAGC TTCGACTGTT AGTGGAGAAT 
AGCCAGCAGA CTTCAGGTTT TGCCCTGGCT TTAGGAAACG TAGTTCATGG ATTGTCTGTG 
TGTGGACATG GAAAAGCTGA AGACTTGGGC AGCAAACTAC TCCCTGCCTG GATCAGAATT 
GTTCTAACAG AGGGCACTCC CACAATGCTT TGTCTGGCAG CTCTTCATGG CATGGTGGCC 
CTGGTAGGCT CTGAAGGGGA TGTAATGCAG CTGAAATCAG AAGCCATCCA GACCTCTCAT 
TTTCAAGGCA GACTTAATGA AGTCATTAGA ACCTTAACTC AGGTCATTAG TGTCTCTGGG 
GTGATTGGTC TCCAGTCAAA TGCAGTCTGG CTTCTTGGAC ATCTTCATCT ATCTACTCTA 
TCCTCAAGTC AAAGTAGAGC CTCTGTTCCT ACTGACTATA GCTATTTGCC TGAAAGCAGT 
TTTATTGGAG CAGCTATTGG CTTCTTCATT ACAGGAGGAA AAAAAGGTCC TGAATCTGTG 
CCTCCTTCCC TTCTTAAAGT AGTGATGAAA CCCATAGCAA CTGTTGGAGA AAGCTACCAA 
TATCCTCCTG TGAACTGGGC TGCACTTCTC TCTCCACTTA TGAGGCTAAA TTTTGGTGAA 
GAGATCCAGC AACTGTGCCT TGAAATTATG GTGACCCAGG CACAGTCATC CCAGAATGCA 
GCTGCACTAT TGGGCTTGTG GGTGACACCA CCACTGATCC ACAGTCTGAG TCTGAATACC 
AAGAGATATC TCCTGATATC TGCACCTCTG TGGATAAAAC ACATCTCTGA TGAACAGATC 
CTGGGTTTTG TTGAAAATTT AATGGTGGCA GTTTTTAAAG CAGCTTCCCC ACTTGGAAAT 
CCTGAGCTAT GCCCGAGTGC TTTACAGGGT CTGAGCCAGG CCATGAAACT GCCCAGCCCT 
GCTCACCACC TCTGGAGTCT GCTCTCTGAA GCTACTGGGA AAATTTTTGA CCTCCTGCCA 
AATAAGATTC GGAGAAAGGA TCTAGAGCTG TATATCAGCA TAGCAAAATG CCTCTTAGAA 
ATGACAGATG ATGATGCCAA TCGGATCGCC CAGGTTACTA AGAGCAACAT AGAAAAGGCT 
GCCTTTGTCA AACTGTACTT AGTCTCTCAA GGACGATTCC CCTTGGTGAA CCTGACCGAT 
ATGCTGAGTG TTGCTGTGCA GCACCGTGAG AAAGAGGTGT TGGCCTGGAT GATTCTGCAC 
AGCTTATACC AGGCACGGAT TGTGAGCCAT GCCAACACGG GCGTTTTGAA GAGAATGGAG 
TGGCTCTTGG AACTGATGGG TTATATTAGA AATGTTGCGT ACCAGTCAAC ATCCTTTCAG 
AATACGGCTC TTGATGAGGC TTTGGACTTC TTCTTGCTGA TATTTGCAAC CGCAGTGGTT 
GCATGGGCCG ACCACACTGC CCCTCTCCTC CTTGGTCTCA GTGCCAGTTG GTTGCCATGG 
CATCAGGAGA ATGGCCCGGC TGGGCCAGTA CCAAGCTTCC TTGGCAGGAG TCCAATGCAC 
AGGGTCACTG TGCAGGAGGT TCTCACTCTC CTTCCCAATA GCATGGCTCT GCTGCTGCAG 
AAAGAGCCAT GGAAGGAAGA GACCCAGAAG TTCATTGACT GGCTATTCAG CATCATGGAA 
AGCCCTAAAG AAGCCCTCTC AGCAAAGTCC AGGGATCTTT TGAAAGCCAC CCTGCTGTCC 
TTGAGAGTTC TCCCAGAGTT TAAGAAGAAA GCTGTATGGA CCAGAGCGTA TGGTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009242665.1
CDS	58..5517
Translation	MDNKGRQEAAHIHVREAKMSDDIRKRFEFPNSLIQSQAAGHLIAAVLKENGFSEKIYQSTNQTPALNLLWEKCCSDNVVVRTACCEGLVALVAQDHAEFSYVLNGILNLIPSTRNTHGLIKAIMKLLQMQALKEGQGGEKNIQRVYTIRNHPHPLITVLEHRPDCWPVFLQQLTAFFQQCPERSEVSCIQIMAPFLWYLYCEPSQLQEYAKLRLALLKVLLQPQVLCDKDQPSILQQQILQLCCDIVPCLQIKDLIQTTEAMMFIEEVYLSLLRHPVFWKIQLTQMTLQLLCVGEVSLKITGECSSSIHLLEHSVELLKEDFPVELVIIGIALLLLQTPASQQKPILNLALKLFSVTEDQKIPKSSLLLVMPILQILSSTALEDCISVDEEGPSRQQLALNLLEMIQQECYRDDHQKLSYKLVCPVTSMYGSVFTAWRILEVMTDSSAASDWLASVESLLPITTVIPVPAFLLLAHLLVEDKGQNLHQILKVTTELAQADSSQVPNLIPVLMFKLGRPLEPILYNDILYTLPKLGVHKVCIGQILRVIQLLGTTPRLRAVTLRLLTSLWEKQDRVYPELQRFMAVSDVPSLSVGKEVQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQATPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDTRPLILKTLSELFSLVPSLTVNTTEYENFKVQVLSFLWTHTQNKDPIVANAAYRSLANFSAGEHTILHLPEKIRPEIPIPEELDDDEDDEDVDLSVPGSCYLKLLSLTPPLVLPALEEFFTSLVKQEMVNMPRGIYHSALKGGVRSDQGKTVAGIPNFILKMYETNKQPGLKPGLAGGMLFCYDVSMYQSKDGKPLNRLMASRGRSFKQTSLALVHEVHIQLSEWHRAIFLPQAWLAYMNRAYHAILQGRLGELELQLKHGKEEPEEVQYKKSTAWLWVRDMLTDEITKAAAKESPVVKGNALLALSSLAVVVSRHEASLSSDSDGLLEVQPNFLSMKEWVSMILDTLLVIVDSHYQPRGQLLSWFYYKSYSGENTASAIARSAAATALSLLVPVFIISCKEKVEEILNMLTARLPGKPSADESQAVQIHMGLALGMFLSRLCEEKLSDICGQEMNLLLMKSLDALENCCFDTSLEYNTGCILGVGLVLSLMSHSSQMQSCVHVTALLRKLSAYLDDSGSQSRTFQEVLAYTLSCVCTSAFSAGIIEATEAEDVMNKLRLLVENSQQTSGFALALGNVVHGLSVCGHGKAEDLGSKLLPAWIRIVLTEGTPTMLCLAALHGMVALVGSEGDVMQLKSEAIQTSHFQGRLNEVIRTLTQVISVSGVIGLQSNAVWLLGHLHLSTLSSSQSRASVPTDYSYLPESSFIGAAIGFFITGGKKGPESVPPSLLKVVMKPIATVGESYQYPPVNWAALLSPLMRLNFGEEIQQLCLEIMVTQAQSSQNAAALLGLWVTPPLIHSLSLNTKRYLLISAPLWIKHISDEQILGFVENLMVAVFKAASPLGNPELCPSALQGLSQAMKLPSPAHHLWSLLSEATGKIFDLLPNKIRRKDLELYISIAKCLLEMTDDDANRIAQVTKSNIEKAAFVKLYLVSQGRFPLVNLTDMLSVAVQHREKEVLAWMILHSLYQARIVSHANTGVLKRMEWLLELMGYIRNVAYQSTSFQNTALDEALDFFLLIFATAVVAWADHTAPLLLGLSASWLPWHQENGPAGPVPSFLGRSPMHRVTVQEVLTLLPNSMALLLQKEPWKEETQKFIDWLFSIMESPKEALSAKSRDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_009244390.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii focadhesin (FOCAD), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009244390.1

ORF Insert Sequence:

ATGGACAACA AAGGAAGGCA AGAAGCTGCA CACATACATG TAAGAGAAGC AAAAATGTCA 
GATGATATCA GGAAAAGGTT TGAATTTCCA AATTCTCTTA TCCAATCACA GGCTGCGGGT 
CATCTTATTG CTGCAGTCCT AAAGGAGAAT GGTTTTTCAG AAAAGATTTA CCAATCTACA 
AATCAGACTC CTGCTTTGAA CTTGCTGTGG GAGAAGTGTT GCAGTGACAA TGTAGTGGTT 
CGAACAGCCT GCTGTGAAGG TCTGGTGGCA CTCGTTGCTC AGGATCATGC AGAGTTCAGC 
TATGTTCTCA ATGGGATACT CAACTTGATT CCATCAACCA GAAATACACA TGGCTTGATA 
AAAGCCATTA TGAAATTACT ACAAATGCAA GCTCTTAAGG AAGGACAAGG TGGGGAAAAG 
AATATTCAGA GGGTATATAC CATTAGAAAT CATCCTCATC CTTTGATCAC TGTGCTTGAA 
CACAGACCTG ATTGTTGGCC AGTGTTTTTG CAGCAGCTGA CAGCATTTTT CCAGCAGTGC 
CCTGAAAGGT CAGAAGTTTC ATGCATTCAA ATAATGGCAC CATTTCTGTG GTATCTGTAT 
TGTGAACCAT CTCAGTTACA AGAATATGCT AAACTCCGAC TAGCCCTGCT GAAAGTCTTA 
CTTCAACCCC AGGTTCTTTG TGACAAGGAT CAACCATCAA TACTGCAACA GCAGATACTT 
CAACTGTGTT GTGACATAGT TCCATGTTTG CAGATAAAAG ATTTGATACA GACAACAGAA 
GCGATGATGT TTATTGAGGA AGTATATTTA AGCCTTTTGC GTCATCCTGT TTTCTGGAAA 
ATTCAGCTTA CCCAGATGAC TCTTCAGCTG CTGTGTGTCG GTGAAGTCAG CTTAAAGATA 
ACTGGTGAAT GTTCATCTTC AATTCACCTC TTAGAGCACA GTGTTGAACT TCTGAAGGAG 
GATTTTCCTG TTGAACTGGT CATAATTGGA ATAGCTTTAC TACTTCTACA GACTCCAGCA 
AGTCAGCAGA AGCCAATCTT AAATCTAGCT TTGAAGCTCT TCTCTGTTAC TGAGGATCAG 
AAAATCCCAA AGTCCTCTCT GCTGCTAGTG ATGCCAATTC TGCAGATACT GTCTTCTACT 
GCCTTGGAAG ACTGTATATC TGTGGATGAA GAAGGTCCCT CTAGGCAGCA GTTGGCTCTA 
AACCTTTTGG AAATGATACA GCAGGAATGT TACAGAGATG ACCACCAAAA GCTCTCCTAC 
AAGCTTGTGT GCCCTGTAAC CAGTATGTAT GGTTCAGTAT TTACAGCCTG GAGGATTCTT 
GAAGTAATGA CAGACTCCTC TGCTGCAAGT GACTGGTTGG CTTCAGTAGA GTCATTGCTT 
CCTATTACTA CTGTGATCCC TGTGCCTGCC TTTCTTCTGC TGGCTCACCT CCTTGTTGAA 
GACAAAGGAC AAAATCTTCA CCAAATACTC AAGGTCACTA CAGAATTAGC CCAAGCAGAT 
TCCTCCCAGG TGCCAAATCT GATTCCAGTT TTGATGTTCA AATTGGGAAG ACCACTGGAA 
CCTATATTAT ATAATGATAT ATTGTATACT TTACCTAAGC TTGGTGTTCA CAAGGTGTGT 
ATAGGACAAA TTCTACGAGT AATACAACTG CTTGGAACCA CACCACGACT AAGAGCTGTC 
ACTTTGCGCT TGCTGACATC TTTGTGGGAA AAGCAGGACC GAGTCTATCC TGAACTGCAG 
CGTTTCATGG CTGTGTCTGA TGTACCTTCT CTTTCGGTGG GCAAGGAAGT CCAATGGGAG 
AAACTGATTG CAAAAGCAGC ATCAATCAGA GATATATGTA AGCAGAGGCC ATATCAACAT 
GGTGCAGATA TGCTGGCAGC TATTTCTCAG GTGTTGAATG AATGCACCAA GCCTGATCAA 
GCTACTCCAG CAGCCTTGGT ATTACAAGGT CTTCATGCAC TCTGTCAAGC TGAGGTTGTT 
TGCATTCGCT CCACTTGGAA TGCTCTCTCT CCAAAGCTGA GTTGTGACAC AAGACCTCTC 
ATTCTGAAGA CACTGAGTGA ACTATTTTCT CTAGTTCCTT CCTTAACAGT CAATACAACT 
GAATATGAGA ATTTTAAAGT TCAAGTCCTC AGCTTCCTCT GGACTCATAC TCAAAACAAG 
GACCCAATTG TGGCAAATGC TGCATATAGA TCCCTGGCCA ATTTTAGTGC AGGAGAACAC 
ACCATTCTTC ATCTGCCTGA AAAGATAAGA CCAGAAATTC CCATTCCTGA AGAGTTAGAT 
GATGATGAAG ATGATGAGGA TGTGGATCTT TCAGTTCCTG GCTCTTGCTA TCTCAAACTG 
TTGTCACTCA CTCCCCCTTT GGTTTTACCA GCTTTGGAGG AATTTTTTAC ATCACTTGTG 
AAGCAAGAAA TGGTGAATAT GCCTCGTGGG ATATATCACT CTGCATTAAA AGGAGGTGTC 
CGCTCAGACC AAGGAAAGAC TGTAGCAGGA ATCCCCAATT TTATATTGAA AATGTATGAA 
ACAAACAAGC AACCAGGATT GAAACCTGGC CTTGCAGGTG GTATGTTATT TTGCTATGAT 
GTTTCCATGT ATCAGAGTAA AGATGGAAAA CCATTGAATA GACTGATGGC CAGCAGAGGG 
CGAAGTTTCA AGCAGACATC ACTTGCTCTT GTACATGAGG TTCATATCCA GCTTTCAGAG 
TGGCACCGTG CGATTTTTCT TCCACAGGCC TGGCTTGCAT ACATGAATCG AGCTTATCAT 
GCCATTTTAC AGGGAAGACT AGGAGAGCTG GAGTTGCAGT TAAAACATGG AAAAGAAGAA 
CCTGAGGAGG TGCAGTACAA AAAAAGCACA GCTTGGCTCT GGGTTAGAGA CATGCTGACT 
GATGAGATCA CCAAGGCAGC TGCAAAGGAG AGTCCGGTAG TGAAAGGCAA TGCGCTGTTA 
GCTCTAAGCA GCCTTGCTGT CGTCGTATCT AGACATGAAG CCAGCCTCTC CTCAGACTCT 
GACGGGCTCC TGGAGGTTCA ACCTAATTTC CTTTCAATGA AAGAGTGGGT TTCCATGATA 
CTTGATACAC TCTTGGTCAT TGTGGATAGC CATTACCAAC CCAGAGGGCA ACTTCTCTCC 
TGGTTTTATT ATAAGTCCTA TTCTGGTGAA AACACAGCTA GTGCCATTGC CCGTTCTGCT 
GCCGCCACGG CTTTGTCTCT CCTTGTGCCA GTTTTCATTA TCTCTTGCAA AGAGAAGGTT 
GAGGAAATCC TGAACATGCT GACTGCCAGG TTACCTGGGA AACCAAGTGC TGATGAGTCT 
CAAGCCGTGC AAATCCACAT GGGCCTTGCT TTAGGGATGT TTCTCTCTCG CTTGTGTGAA 
GAGAAACTCA GTGATATATG TGGCCAAGAG ATGAACCTTC TTCTGATGAA GTCGTTGGAT 
GCCCTGGAAA ATTGCTGCTT TGACACTAGT CTTGAATACA ACACGGGCTG TATATTGGGA 
GTTGGACTTG TTCTGTCCCT CATGAGTCAC AGCAGCCAAA TGCAGTCCTG CGTTCACGTG 
ACAGCATTGC TTCGGAAGCT GTCTGCATAC CTAGATGACA GTGGGAGCCA GAGCAGAACG 
TTTCAGGAGG TCCTTGCCTA CACACTTAGC TGTGTATGTA CATCAGCGTT CAGTGCTGGA 
ATTATTGAGG CTACAGAGGC TGAGGATGTT ATGAACAAGC TTCGACTGTT AGTGGAGAAT 
AGCCAGCAGA CTTCAGGTTT TGCCCTGGCT TTAGGAAACG TAGTTCATGG ATTGTCTGTG 
TGTGGACATG GAAAAGCTGA AGACTTGGGC AGCAAACTAC TCCCTGCCTG GATCAGAATT 
GTTCTAACAG AGGGCACTCC CACAATGCTT TGTCTGGCAG CTCTTCATGG CATGGTGGCC 
CTGGTAGGCT CTGAAGGGGA TGTAATGCAG CTGAAATCAG AAGCCATCCA GACCTCTCAT 
TTTCAAGGCA GACTTAATGA AGTCATTAGA ACCTTAACTC AGGTCATTAG TGTCTCTGGG 
GTGATTGGTC TCCAGTCAAA TGCAGTCTGG CTTCTTGGAC ATCTTCATCT ATCTACTCTA 
TCCTCAAGTC AAAGTAGAGC CTCTGTTCCT ACTGACTATA GCTATTTGCC TGAAAGCAGT 
TTTATTGGAG CAGCTATTGG CTTCTTCATT ACAGGAGGAA AAAAAGGTCC TGAATCTGTG 
CCTCCTTCCC TTCTTAAAGT AGTGATGAAA CCCATAGCAA CTGTTGGAGA AAGCTACCAA 
TATCCTCCTG TGAACTGGGC TGCACTTCTC TCTCCACTTA TGAGGCTAAA TTTTGGTGAA 
GAGATCCAGC AACTGTGCCT TGAAATTATG GTGACCCAGG CACAGTCATC CCAGAATGCA 
GCTGCACTAT TGGGCTTGTG GGTGACACCA CCACTGATCC ACAGTCTGAG TCTGAATACC 
AAGAGATATC TCCTGATATC TGCACCTCTG TGGATAAAAC ACATCTCTGA TGAACAGATC 
CTGGGTTTTG TTGAAAATTT AATGGTGGCA GTTTTTAAAG CAGCTTCCCC ACTTGGAAAT 
CCTGAGCTAT GCCCGAGTGC TTTACAGGGT CTGAGCCAGG CCATGAAACT GCCCAGCCCT 
GCTCACCACC TCTGGAGTCT GCTCTCTGAA GCTACTGGGA AAATTTTTGA CCTCCTGCCA 
AATAAGATTC GGAGAAAGGA TCTAGAGCTG TATATCAGCA TAGCAAAATG CCTCTTAGAA 
ATGACAGATG ATGATGCCAA TCGGATCGCC CAGGTTACTA AGAGCAACAT AGAAAAGGCT 
GCCTTTGTCA AACTGTACTT AGTCTCTCAA GGACGATTCC CCTTGGTGAA CCTGACCGAT 
ATGCTGAGTG TTGCTGTGCA GCACCGTGAG AAAGAGGTGT TGGCCTGGAT GATTCTGCAC 
AGCTTATACC AGGCACGGAT TGTGAGCCAT GCCAACACGG GCGTTTTGAA GAGAATGGAG 
TGGCTCTTGG AACTGATGGG TTATATTAGA AATGTTGCGT ACCAGTCAAC ATCCTTTCAG 
AATACGGCTC TTGATGAGGC TTTGGACTTC TTCTTGCTGA TATTTGCAAC CGCAGTGGTT 
GCATGGGCCG ACCACACTGC CCCTCTCCTC CTTGGTCTCA GTGCCAGTTG GTTGCCATGG 
CATCAGGAGA ATGGCCCGGC TGGGCCAGTA CCAAGCTTCC TTGGCAGGAG TCCAATGCAC 
AGGGTCACTG TGCAGGAGGT TCTCACTCTC CTTCCCAATA GCATGGCTCT GCTGCTGCAG 
AAAGAGCCAT GGAAGGAAGA GACCCAGAAG TTCATTGACT GGCTATTCAG CATCATGGAA 
AGCCCTAAAG AAGCCCTCTC AGCAAAGTCC AGGGATCTTT TGAAAGCCAC CCTGCTGTCC 
TTGAGAGTTC TCCCAGAGTT TAAGAAGAAA GCTGTATGGA CCAGAGCGTA TGGTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FOCAD ( XM_009244391.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009242666.1 Pongo abelii focadhesin (FOCAD), transcript variant X3, mRNA.

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CloneID	OSa28573
Clone ID Related Accession (Same CDS sequence)	XM_009244391.1
Accession Version	XM_009244391.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-22
Organism	Pongo abelii(Sumatran orangutan)
Product	focadhesin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002883424.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pongo abelii Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAATA ATAACTATAT TTGGATGAAG TGGACCAATG AACCATCCAC TGAAGCTCCT 
AAACTGGAGA TTTATGCTTT CGAAGAAGCT GCACACATAC ATGTAAGAGA AGCAAAAATG 
TCAGATGATA TCAGGAAAAG GTTTGAATTT CCAAATTCTC TTATCCAATC ACAGGCTGCG 
GGTCATCTTA TTGCTGCAGT CCTAAAGGAG AATGGTTTTT CAGAAAAGAT TTACCAATCT 
ACAAATCAGA CTCCTGCTTT GAACTTGCTG TGGGAGAAGT GTTGCAGTGA CAATGTAGTG 
GTTCGAACAG CCTGCTGTGA AGGTCTGGTG GCACTCGTTG CTCAGGATCA TGCAGAGTTC 
AGCTATGTTC TCAATGGGAT ACTCAACTTG ATTCCATCAA CCAGAAATAC ACATGGCTTG 
ATAAAAGCCA TTATGAAATT ACTACAAATG CAAGCTCTTA AGGAAGGACA AGGTGGGGAA 
AAGAATATTC AGAGGGTATA TACCATTAGA AATCATCCTC ATCCTTTGAT CACTGTGCTT 
GAACACAGAC CTGATTGTTG GCCAGTGTTT TTGCAGCAGC TGACAGCATT TTTCCAGCAG 
TGCCCTGAAA GGTCAGAAGT TTCATGCATT CAAATAATGG CACCATTTCT GTGGTATCTG 
TATTGTGAAC CATCTCAGTT ACAAGAATAT GCTAAACTCC GACTAGCCCT GCTGAAAGTC 
TTACTTCAAC CCCAGGTTCT TTGTGACAAG GATCAACCAT CAATACTGCA ACAGCAGATA 
CTTCAACTGT GTTGTGACAT AGTTCCATGT TTGCAGATAA AAGATTTGAT ACAGACAACA 
GAAGCGATGA TGTTTATTGA GGAAGTATAT TTAAGCCTTT TGCGTCATCC TGTTTTCTGG 
AAAATTCAGC TTACCCAGAT GACTCTTCAG CTGCTGTGTG TCGGTGAAGT CAGCTTAAAG 
ATAACTGGTG AATGTTCATC TTCAATTCAC CTCTTAGAGC ACAGTGTTGA ACTTCTGAAG 
GAGGATTTTC CTGTTGAACT GGTCATAATT GGAATAGCTT TACTACTTCT ACAGACTCCA 
GCAAGTCAGC AGAAGCCAAT CTTAAATCTA GCTTTGAAGC TCTTCTCTGT TACTGAGGAT 
CAGAAAATCC CAAAGTCCTC TCTGCTGCTA GTGATGCCAA TTCTGCAGAT ACTGTCTTCT 
ACTGCCTTGG AAGACTGTAT ATCTGTGGAT GAAGAAGGTC CCTCTAGGCA GCAGTTGGCT 
CTAAACCTTT TGGAAATGAT ACAGCAGGAA TGTTACAGAG ATGACCACCA AAAGCTCTCC 
TACAAGCTTG TGTGCCCTGT AACCAGTATG TATGGTTCAG TATTTACAGC CTGGAGGATT 
CTTGAAGTAA TGACAGACTC CTCTGCTGCA AGTGACTGGT TGGCTTCAGT AGAGTCATTG 
CTTCCTATTA CTACTGTGAT CCCTGTGCCT GCCTTTCTTC TGCTGGCTCA CCTCCTTGTT 
GAAGACAAAG GACAAAATCT TCACCAAATA CTCAAGGTCA CTACAGAATT AGCCCAAGCA 
GATTCCTCCC AGGTGCCAAA TCTGATTCCA GTTTTGATGT TCAAATTGGG AAGACCACTG 
GAACCTATAT TATATAATGA TATATTGTAT ACTTTACCTA AGCTTGGTGT TCACAAGGTG 
TGTATAGGAC AAATTCTACG AGTAATACAA CTGCTTGGAA CCACACCACG ACTAAGAGCT 
GTCACTTTGC GCTTGCTGAC ATCTTTGTGG GAAAAGCAGG ACCGAGTCTA TCCTGAACTG 
CAGCGTTTCA TGGCTGTGTC TGATGTACCT TCTCTTTCGG TGGGCAAGGA AGTCCAATGG 
GAGAAACTGA TTGCAAAAGC AGCATCAATC AGAGATATAT GTAAGCAGAG GCCATATCAA 
CATGGTGCAG ATATGCTGGC AGCTATTTCT CAGGTGTTGA ATGAATGCAC CAAGCCTGAT 
CAAGCTACTC CAGCAGCCTT GGTATTACAA GGTCTTCATG CACTCTGTCA AGCTGAGGTT 
GTTTGCATTC GCTCCACTTG GAATGCTCTC TCTCCAAAGC TGAGTTGTGA CACAAGACCT 
CTCATTCTGA AGACACTGAG TGAACTATTT TCTCTAGTTC CTTCCTTAAC AGTCAATACA 
ACTGAATATG AGAATTTTAA AGTTCAAGTC CTCAGCTTCC TCTGGACTCA TACTCAAAAC 
AAGGACCCAA TTGTGGCAAA TGCTGCATAT AGATCCCTGG CCAATTTTAG TGCAGGAGAA 
CACACCATTC TTCATCTGCC TGAAAAGATA AGACCAGAAA TTCCCATTCC TGAAGAGTTA 
GATGATGATG AAGATGATGA GGATGTGGAT CTTTCAGTTC CTGGCTCTTG CTATCTCAAA 
CTGTTGTCAC TCACTCCCCC TTTGGTTTTA CCAGCTTTGG AGGAATTTTT TACATCACTT 
GTGAAGCAAG AAATGGTGAA TATGCCTCGT GGGATATATC ACTCTGCATT AAAAGGAGGT 
GTCCGCTCAG ACCAAGGAAA GACTGTAGCA GGAATCCCCA ATTTTATATT GAAAATGTAT 
GAAACAAACA AGCAACCAGG ATTGAAACCT GGCCTTGCAG GTGGTATGTT ATTTTGCTAT 
GATGTTTCCA TGTATCAGAG TAAAGATGGA AAACCATTGA ATAGACTGAT GGCCAGCAGA 
GGGCGAAGTT TCAAGCAGAC ATCACTTGCT CTTGTACATG AGGTTCATAT CCAGCTTTCA 
GAGTGGCACC GTGCGATTTT TCTTCCACAG GCCTGGCTTG CATACATGAA TCGAGCTTAT 
CATGCCATTT TACAGGGAAG ACTAGGAGAG CTGGAGTTGC AGTTAAAACA TGGAAAAGAA 
GAACCTGAGG AGGTGCAGTA CAAAAAAAGC ACAGCTTGGC TCTGGGTTAG AGACATGCTG 
ACTGATGAGA TCACCAAGGC AGCTGCAAAG GAGAGTCCGG TAGTGAAAGG CAATGCGCTG 
TTAGCTCTAA GCAGCCTTGC TGTCGTCGTA TCTAGACATG AAGCCAGCCT CTCCTCAGAC 
TCTGACGGGC TCCTGGAGGT TCAACCTAAT TTCCTTTCAA TGAAAGAGTG GGTTTCCATG 
ATACTTGATA CACTCTTGGT CATTGTGGAT AGCCATTACC AACCCAGAGG GCAACTTCTC 
TCCTGGTTTT ATTATAAGTC CTATTCTGGT GAAAACACAG CTAGTGCCAT TGCCCGTTCT 
GCTGCCGCCA CGGCTTTGTC TCTCCTTGTG CCAGTTTTCA TTATCTCTTG CAAAGAGAAG 
GTTGAGGAAA TCCTGAACAT GCTGACTGCC AGGTTACCTG GGAAACCAAG TGCTGATGAG 
TCTCAAGCCG TGCAAATCCA CATGGGCCTT GCTTTAGGGA TGTTTCTCTC TCGCTTGTGT 
GAAGAGAAAC TCAGTGATAT ATGTGGCCAA GAGATGAACC TTCTTCTGAT GAAGTCGTTG 
GATGCCCTGG AAAATTGCTG CTTTGACACT AGTCTTGAAT ACAACACGGG CTGTATATTG 
GGAGTTGGAC TTGTTCTGTC CCTCATGAGT CACAGCAGCC AAATGCAGTC CTGCGTTCAC 
GTGACAGCAT TGCTTCGGAA GCTGTCTGCA TACCTAGATG ACAGTGGGAG CCAGAGCAGA 
ACGTTTCAGG AGGTCCTTGC CTACACACTT AGCTGTGTAT GTACATCAGC GTTCAGTGCT 
GGAATTATTG AGGCTACAGA GGCTGAGGAT GTTATGAACA AGCTTCGACT GTTAGTGGAG 
AATAGCCAGC AGACTTCAGG TTTTGCCCTG GCTTTAGGAA ACGTAGTTCA TGGATTGTCT 
GTGTGTGGAC ATGGAAAAGC TGAAGACTTG GGCAGCAAAC TACTCCCTGC CTGGATCAGA 
ATTGTTCTAA CAGAGGGCAC TCCCACAATG CTTTGTCTGG CAGCTCTTCA TGGCATGGTG 
GCCCTGGTAG GCTCTGAAGG GGATGTAATG CAGGTCATTA GTGTCTCTGG GGTGATTGGT 
CTCCAGTCAA ATGCAGTCTG GCTTCTTGGA CATCTTCATC TATCTACTCT ATCCTCAAGT 
CAAAGTAGAG CCTCTGTTCC TACTGACTAT AGCTATTTGC CTGAAAGCAG TTTTATTGGA 
GCAGCTATTG GCTTCTTCAT TACAGGAGGA AAAAAAGGTC CTGAATCTGT GCCTCCTTCC 
CTTCTTAAAG TAGTGATGAA ACCCATAGCA ACTGTTGGAG AAAGCTACCA ATATCCTCCT 
GTGAACTGGG CTGCACTTCT CTCTCCACTT ATGAGGCTAA ATTTTGGTGA AGAGATCCAG 
CAACTGTGCC TTGAAATTAT GGTGACCCAG GCACAGTCAT CCCAGAATGC AGCTGCACTA 
TTGGGCTTGT GGGTGACACC ACCACTGATC CACAGTCTGA GTCTGAATAC CAAGAGATAT 
CTCCTGATAT CTGCACCTCT GTGGATAAAA CACATCTCTG ATGAACAGAT CCTGGGTTTT 
GTTGAAAATT TAATGGTGGC AGTTTTTAAA GCAGCTTCCC CACTTGGAAA TCCTGAGCTA 
TGCCCGAGTG CTTTACAGGG TCTGAGCCAG GCCATGAAAC TGCCCAGCCC TGCTCACCAC 
CTCTGGAGTC TGCTCTCTGA AGCTACTGGG AAAATTTTTG ACCTCCTGCC AAATAAGATT 
CGGAGAAAGG ATCTAGAGCT GTATATCAGC ATAGCAAAAT GCCTCTTAGA AATGACAGAT 
GATGATGCCA ATCGGATCGC CCAGGTTACT AAGAGCAACA TAGAAAAGGC TGCCTTTGTC 
AAACTGTACT TAGTCTCTCA AGGACGATTC CCCTTGGTGA ACCTGACCGA TATGCTGAGT 
GTTGCTGTGC AGCACCGTGA GAAAGAGGTG TTGGCCTGGA TGATTCTGCA CAGCTTATAC 
CAGGCACGGA TTGTGAGCCA TGCCAACACG GGCGTTTTGA AGAGAATGGA GTGGCTCTTG 
GAACTGATGG GTTATATTAG AAATGTTGCG TACCAGTCAA CATCCTTTCA GAATACGGCT 
CTTGATGAGG CTTTGGACTT CTTCTTGCTG ATATTTGCAA CCGCAGTGGT TGCATGGGCC 
GACCACACTG CCCCTCTCCT CCTTGGTCTC AGTGCCAGTT GGTTGCCATG GCATCAGGAG 
AATGGCCCGG CTGGGCCAGT ACCAAGCTTC CTTGGCAGGA GTCCAATGCA CAGGGTCACT 
GTGCAGGAGG TTCTCACTCT CCTTCCCAAT AGCATGGCTC TGCTGCTGCA GAAAGAGCCA 
TGGAAGGAAG AGACCCAGAA GTTCATTGAC TGGCTATTCA GCATCATGGA AAGCCCTAAA 
GAAGCCCTCT CAGCAAAGTC CAGGGATCTT TTGAAAGCCA CCCTGCTGTC CTTGAGAGTT 
CTCCCAGAGT TTAAGAAGAA AGCTGTATGG ACCAGAGCGT ATGGTTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009242666.1
CDS	66..5516
Translation	MKNNNYIWMKWTNEPSTEAPKLEIYAFEEAAHIHVREAKMSDDIRKRFEFPNSLIQSQAAGHLIAAVLKENGFSEKIYQSTNQTPALNLLWEKCCSDNVVVRTACCEGLVALVAQDHAEFSYVLNGILNLIPSTRNTHGLIKAIMKLLQMQALKEGQGGEKNIQRVYTIRNHPHPLITVLEHRPDCWPVFLQQLTAFFQQCPERSEVSCIQIMAPFLWYLYCEPSQLQEYAKLRLALLKVLLQPQVLCDKDQPSILQQQILQLCCDIVPCLQIKDLIQTTEAMMFIEEVYLSLLRHPVFWKIQLTQMTLQLLCVGEVSLKITGECSSSIHLLEHSVELLKEDFPVELVIIGIALLLLQTPASQQKPILNLALKLFSVTEDQKIPKSSLLLVMPILQILSSTALEDCISVDEEGPSRQQLALNLLEMIQQECYRDDHQKLSYKLVCPVTSMYGSVFTAWRILEVMTDSSAASDWLASVESLLPITTVIPVPAFLLLAHLLVEDKGQNLHQILKVTTELAQADSSQVPNLIPVLMFKLGRPLEPILYNDILYTLPKLGVHKVCIGQILRVIQLLGTTPRLRAVTLRLLTSLWEKQDRVYPELQRFMAVSDVPSLSVGKEVQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKPDQATPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDTRPLILKTLSELFSLVPSLTVNTTEYENFKVQVLSFLWTHTQNKDPIVANAAYRSLANFSAGEHTILHLPEKIRPEIPIPEELDDDEDDEDVDLSVPGSCYLKLLSLTPPLVLPALEEFFTSLVKQEMVNMPRGIYHSALKGGVRSDQGKTVAGIPNFILKMYETNKQPGLKPGLAGGMLFCYDVSMYQSKDGKPLNRLMASRGRSFKQTSLALVHEVHIQLSEWHRAIFLPQAWLAYMNRAYHAILQGRLGELELQLKHGKEEPEEVQYKKSTAWLWVRDMLTDEITKAAAKESPVVKGNALLALSSLAVVVSRHEASLSSDSDGLLEVQPNFLSMKEWVSMILDTLLVIVDSHYQPRGQLLSWFYYKSYSGENTASAIARSAAATALSLLVPVFIISCKEKVEEILNMLTARLPGKPSADESQAVQIHMGLALGMFLSRLCEEKLSDICGQEMNLLLMKSLDALENCCFDTSLEYNTGCILGVGLVLSLMSHSSQMQSCVHVTALLRKLSAYLDDSGSQSRTFQEVLAYTLSCVCTSAFSAGIIEATEAEDVMNKLRLLVENSQQTSGFALALGNVVHGLSVCGHGKAEDLGSKLLPAWIRIVLTEGTPTMLCLAALHGMVALVGSEGDVMQVISVSGVIGLQSNAVWLLGHLHLSTLSSSQSRASVPTDYSYLPESSFIGAAIGFFITGGKKGPESVPPSLLKVVMKPIATVGESYQYPPVNWAALLSPLMRLNFGEEIQQLCLEIMVTQAQSSQNAAALLGLWVTPPLIHSLSLNTKRYLLISAPLWIKHISDEQILGFVENLMVAVFKAASPLGNPELCPSALQGLSQAMKLPSPAHHLWSLLSEATGKIFDLLPNKIRRKDLELYISIAKCLLEMTDDDANRIAQVTKSNIEKAAFVKLYLVSQGRFPLVNLTDMLSVAVQHREKEVLAWMILHSLYQARIVSHANTGVLKRMEWLLELMGYIRNVAYQSTSFQNTALDEALDFFLLIFATAVVAWADHTAPLLLGLSASWLPWHQENGPAGPVPSFLGRSPMHRVTVQEVLTLLPNSMALLLQKEPWKEETQKFIDWLFSIMESPKEALSAKSRDLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_009244391.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii focadhesin (FOCAD), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009244391.1

ORF Insert Sequence:

ATGAAAAATA ATAACTATAT TTGGATGAAG TGGACCAATG AACCATCCAC TGAAGCTCCT 
AAACTGGAGA TTTATGCTTT CGAAGAAGCT GCACACATAC ATGTAAGAGA AGCAAAAATG 
TCAGATGATA TCAGGAAAAG GTTTGAATTT CCAAATTCTC TTATCCAATC ACAGGCTGCG 
GGTCATCTTA TTGCTGCAGT CCTAAAGGAG AATGGTTTTT CAGAAAAGAT TTACCAATCT 
ACAAATCAGA CTCCTGCTTT GAACTTGCTG TGGGAGAAGT GTTGCAGTGA CAATGTAGTG 
GTTCGAACAG CCTGCTGTGA AGGTCTGGTG GCACTCGTTG CTCAGGATCA TGCAGAGTTC 
AGCTATGTTC TCAATGGGAT ACTCAACTTG ATTCCATCAA CCAGAAATAC ACATGGCTTG 
ATAAAAGCCA TTATGAAATT ACTACAAATG CAAGCTCTTA AGGAAGGACA AGGTGGGGAA 
AAGAATATTC AGAGGGTATA TACCATTAGA AATCATCCTC ATCCTTTGAT CACTGTGCTT 
GAACACAGAC CTGATTGTTG GCCAGTGTTT TTGCAGCAGC TGACAGCATT TTTCCAGCAG 
TGCCCTGAAA GGTCAGAAGT TTCATGCATT CAAATAATGG CACCATTTCT GTGGTATCTG 
TATTGTGAAC CATCTCAGTT ACAAGAATAT GCTAAACTCC GACTAGCCCT GCTGAAAGTC 
TTACTTCAAC CCCAGGTTCT TTGTGACAAG GATCAACCAT CAATACTGCA ACAGCAGATA 
CTTCAACTGT GTTGTGACAT AGTTCCATGT TTGCAGATAA AAGATTTGAT ACAGACAACA 
GAAGCGATGA TGTTTATTGA GGAAGTATAT TTAAGCCTTT TGCGTCATCC TGTTTTCTGG 
AAAATTCAGC TTACCCAGAT GACTCTTCAG CTGCTGTGTG TCGGTGAAGT CAGCTTAAAG 
ATAACTGGTG AATGTTCATC TTCAATTCAC CTCTTAGAGC ACAGTGTTGA ACTTCTGAAG 
GAGGATTTTC CTGTTGAACT GGTCATAATT GGAATAGCTT TACTACTTCT ACAGACTCCA 
GCAAGTCAGC AGAAGCCAAT CTTAAATCTA GCTTTGAAGC TCTTCTCTGT TACTGAGGAT 
CAGAAAATCC CAAAGTCCTC TCTGCTGCTA GTGATGCCAA TTCTGCAGAT ACTGTCTTCT 
ACTGCCTTGG AAGACTGTAT ATCTGTGGAT GAAGAAGGTC CCTCTAGGCA GCAGTTGGCT 
CTAAACCTTT TGGAAATGAT ACAGCAGGAA TGTTACAGAG ATGACCACCA AAAGCTCTCC 
TACAAGCTTG TGTGCCCTGT AACCAGTATG TATGGTTCAG TATTTACAGC CTGGAGGATT 
CTTGAAGTAA TGACAGACTC CTCTGCTGCA AGTGACTGGT TGGCTTCAGT AGAGTCATTG 
CTTCCTATTA CTACTGTGAT CCCTGTGCCT GCCTTTCTTC TGCTGGCTCA CCTCCTTGTT 
GAAGACAAAG GACAAAATCT TCACCAAATA CTCAAGGTCA CTACAGAATT AGCCCAAGCA 
GATTCCTCCC AGGTGCCAAA TCTGATTCCA GTTTTGATGT TCAAATTGGG AAGACCACTG 
GAACCTATAT TATATAATGA TATATTGTAT ACTTTACCTA AGCTTGGTGT TCACAAGGTG 
TGTATAGGAC AAATTCTACG AGTAATACAA CTGCTTGGAA CCACACCACG ACTAAGAGCT 
GTCACTTTGC GCTTGCTGAC ATCTTTGTGG GAAAAGCAGG ACCGAGTCTA TCCTGAACTG 
CAGCGTTTCA TGGCTGTGTC TGATGTACCT TCTCTTTCGG TGGGCAAGGA AGTCCAATGG 
GAGAAACTGA TTGCAAAAGC AGCATCAATC AGAGATATAT GTAAGCAGAG GCCATATCAA 
CATGGTGCAG ATATGCTGGC AGCTATTTCT CAGGTGTTGA ATGAATGCAC CAAGCCTGAT 
CAAGCTACTC CAGCAGCCTT GGTATTACAA GGTCTTCATG CACTCTGTCA AGCTGAGGTT 
GTTTGCATTC GCTCCACTTG GAATGCTCTC TCTCCAAAGC TGAGTTGTGA CACAAGACCT 
CTCATTCTGA AGACACTGAG TGAACTATTT TCTCTAGTTC CTTCCTTAAC AGTCAATACA 
ACTGAATATG AGAATTTTAA AGTTCAAGTC CTCAGCTTCC TCTGGACTCA TACTCAAAAC 
AAGGACCCAA TTGTGGCAAA TGCTGCATAT AGATCCCTGG CCAATTTTAG TGCAGGAGAA 
CACACCATTC TTCATCTGCC TGAAAAGATA AGACCAGAAA TTCCCATTCC TGAAGAGTTA 
GATGATGATG AAGATGATGA GGATGTGGAT CTTTCAGTTC CTGGCTCTTG CTATCTCAAA 
CTGTTGTCAC TCACTCCCCC TTTGGTTTTA CCAGCTTTGG AGGAATTTTT TACATCACTT 
GTGAAGCAAG AAATGGTGAA TATGCCTCGT GGGATATATC ACTCTGCATT AAAAGGAGGT 
GTCCGCTCAG ACCAAGGAAA GACTGTAGCA GGAATCCCCA ATTTTATATT GAAAATGTAT 
GAAACAAACA AGCAACCAGG ATTGAAACCT GGCCTTGCAG GTGGTATGTT ATTTTGCTAT 
GATGTTTCCA TGTATCAGAG TAAAGATGGA AAACCATTGA ATAGACTGAT GGCCAGCAGA 
GGGCGAAGTT TCAAGCAGAC ATCACTTGCT CTTGTACATG AGGTTCATAT CCAGCTTTCA 
GAGTGGCACC GTGCGATTTT TCTTCCACAG GCCTGGCTTG CATACATGAA TCGAGCTTAT 
CATGCCATTT TACAGGGAAG ACTAGGAGAG CTGGAGTTGC AGTTAAAACA TGGAAAAGAA 
GAACCTGAGG AGGTGCAGTA CAAAAAAAGC ACAGCTTGGC TCTGGGTTAG AGACATGCTG 
ACTGATGAGA TCACCAAGGC AGCTGCAAAG GAGAGTCCGG TAGTGAAAGG CAATGCGCTG 
TTAGCTCTAA GCAGCCTTGC TGTCGTCGTA TCTAGACATG AAGCCAGCCT CTCCTCAGAC 
TCTGACGGGC TCCTGGAGGT TCAACCTAAT TTCCTTTCAA TGAAAGAGTG GGTTTCCATG 
ATACTTGATA CACTCTTGGT CATTGTGGAT AGCCATTACC AACCCAGAGG GCAACTTCTC 
TCCTGGTTTT ATTATAAGTC CTATTCTGGT GAAAACACAG CTAGTGCCAT TGCCCGTTCT 
GCTGCCGCCA CGGCTTTGTC TCTCCTTGTG CCAGTTTTCA TTATCTCTTG CAAAGAGAAG 
GTTGAGGAAA TCCTGAACAT GCTGACTGCC AGGTTACCTG GGAAACCAAG TGCTGATGAG 
TCTCAAGCCG TGCAAATCCA CATGGGCCTT GCTTTAGGGA TGTTTCTCTC TCGCTTGTGT 
GAAGAGAAAC TCAGTGATAT ATGTGGCCAA GAGATGAACC TTCTTCTGAT GAAGTCGTTG 
GATGCCCTGG AAAATTGCTG CTTTGACACT AGTCTTGAAT ACAACACGGG CTGTATATTG 
GGAGTTGGAC TTGTTCTGTC CCTCATGAGT CACAGCAGCC AAATGCAGTC CTGCGTTCAC 
GTGACAGCAT TGCTTCGGAA GCTGTCTGCA TACCTAGATG ACAGTGGGAG CCAGAGCAGA 
ACGTTTCAGG AGGTCCTTGC CTACACACTT AGCTGTGTAT GTACATCAGC GTTCAGTGCT 
GGAATTATTG AGGCTACAGA GGCTGAGGAT GTTATGAACA AGCTTCGACT GTTAGTGGAG 
AATAGCCAGC AGACTTCAGG TTTTGCCCTG GCTTTAGGAA ACGTAGTTCA TGGATTGTCT 
GTGTGTGGAC ATGGAAAAGC TGAAGACTTG GGCAGCAAAC TACTCCCTGC CTGGATCAGA 
ATTGTTCTAA CAGAGGGCAC TCCCACAATG CTTTGTCTGG CAGCTCTTCA TGGCATGGTG 
GCCCTGGTAG GCTCTGAAGG GGATGTAATG CAGGTCATTA GTGTCTCTGG GGTGATTGGT 
CTCCAGTCAA ATGCAGTCTG GCTTCTTGGA CATCTTCATC TATCTACTCT ATCCTCAAGT 
CAAAGTAGAG CCTCTGTTCC TACTGACTAT AGCTATTTGC CTGAAAGCAG TTTTATTGGA 
GCAGCTATTG GCTTCTTCAT TACAGGAGGA AAAAAAGGTC CTGAATCTGT GCCTCCTTCC 
CTTCTTAAAG TAGTGATGAA ACCCATAGCA ACTGTTGGAG AAAGCTACCA ATATCCTCCT 
GTGAACTGGG CTGCACTTCT CTCTCCACTT ATGAGGCTAA ATTTTGGTGA AGAGATCCAG 
CAACTGTGCC TTGAAATTAT GGTGACCCAG GCACAGTCAT CCCAGAATGC AGCTGCACTA 
TTGGGCTTGT GGGTGACACC ACCACTGATC CACAGTCTGA GTCTGAATAC CAAGAGATAT 
CTCCTGATAT CTGCACCTCT GTGGATAAAA CACATCTCTG ATGAACAGAT CCTGGGTTTT 
GTTGAAAATT TAATGGTGGC AGTTTTTAAA GCAGCTTCCC CACTTGGAAA TCCTGAGCTA 
TGCCCGAGTG CTTTACAGGG TCTGAGCCAG GCCATGAAAC TGCCCAGCCC TGCTCACCAC 
CTCTGGAGTC TGCTCTCTGA AGCTACTGGG AAAATTTTTG ACCTCCTGCC AAATAAGATT 
CGGAGAAAGG ATCTAGAGCT GTATATCAGC ATAGCAAAAT GCCTCTTAGA AATGACAGAT 
GATGATGCCA ATCGGATCGC CCAGGTTACT AAGAGCAACA TAGAAAAGGC TGCCTTTGTC 
AAACTGTACT TAGTCTCTCA AGGACGATTC CCCTTGGTGA ACCTGACCGA TATGCTGAGT 
GTTGCTGTGC AGCACCGTGA GAAAGAGGTG TTGGCCTGGA TGATTCTGCA CAGCTTATAC 
CAGGCACGGA TTGTGAGCCA TGCCAACACG GGCGTTTTGA AGAGAATGGA GTGGCTCTTG 
GAACTGATGG GTTATATTAG AAATGTTGCG TACCAGTCAA CATCCTTTCA GAATACGGCT 
CTTGATGAGG CTTTGGACTT CTTCTTGCTG ATATTTGCAA CCGCAGTGGT TGCATGGGCC 
GACCACACTG CCCCTCTCCT CCTTGGTCTC AGTGCCAGTT GGTTGCCATG GCATCAGGAG 
AATGGCCCGG CTGGGCCAGT ACCAAGCTTC CTTGGCAGGA GTCCAATGCA CAGGGTCACT 
GTGCAGGAGG TTCTCACTCT CCTTCCCAAT AGCATGGCTC TGCTGCTGCA GAAAGAGCCA 
TGGAAGGAAG AGACCCAGAA GTTCATTGAC TGGCTATTCA GCATCATGGA AAGCCCTAAA 
GAAGCCCTCT CAGCAAAGTC CAGGGATCTT TTGAAAGCCA CCCTGCTGTC CTTGAGAGTT 
CTCCCAGAGT TTAAGAAGAA AGCTGTATGG ACCAGAGCGT ATGGTTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	FOCAD
Entrez Gene ID	100441453
Full Name	focadhesin
Gene Type	protein-coding
Organism	Pongo abelii(Sumatran orangutan)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

FOCAD cDNA ORF clone, Pongo abelii(Sumatran orangutan)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

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Target ORF information:

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