LOC7459484 cDNA ORF clone, Populus trichocarpa(black cottonwood)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC7459484
Entrez Gene ID	7459484
Full Name	hypothetical protein
Synonyms	POPTRDRAFT_756271
Gene Type	protein-coding
Organism	Populus trichocarpa(black cottonwood)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024595574.1	XP_024451342.1	chromatin modification-related protein EAF1 B isoform X1
XM_024595576.1	XP_024451344.1	chromatin modification-related protein EAF1 B isoform X3
XM_024595577.1	XP_024451345.1	chromatin modification-related protein EAF1 B isoform X4
XM_024595575.1	XP_024451343.1	chromatin modification-related protein EAF1 B isoform X2
XM_002303006.2	XP_002303042.2	hypothetical protein
XM_024595573.1	XP_024451341.1	chromatin modification-related protein EAF1 B isoform X1
XM_024595571.1	XP_024451339.1	chromatin modification-related protein EAF1 B isoform X1
XM_024595572.1	XP_024451340.1	chromatin modification-related protein EAF1 B isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, Schein J, Sterck L, Aerts A, Bhalerao RR, Bhalerao RP, Blaudez D, Boerjan W, Brun A, Brunner A, Busov V, Campbell M, Carlson J, Chalot M, Chapman J, Chen GL, Cooper D, Coutinho PM, Couturier J, Covert S, Cronk Q, Cunningham R, Davis J, Degroeve S, D?jardin A, Depamphilis C, Detter J, Dirks B, Dubchak I, Duplessis S, Ehlting J, Ellis B, Gendler K, Goodstein D, Gribskov M, Grimwood J, Groover A, Gunter L, Hamberger B, Heinze B, Helariutta Y, Henrissat B, Holligan D, Holt R, Huang W, Islam-Faridi N, Jones S, Jones-Rhoades M, Jorgensen R, Joshi C, Kangasj?rvi J, Karlsson J, Kelleher C, Kirkpatrick R, Kirst M, Kohler A, Kalluri U, Larimer F, Leebens-Mack J, Lepl? JC, Locascio P, Lou Y, Lucas S, Martin F, Montanini B, Napoli C, Nelson DR, Nelson C, Nieminen K, Nilsson O, Pereda V, Peter G, Philippe R, Pilate G, Poliakov A, Razumovskaya J, Richardson P, Rinaldi C, Ritland K, Rouz? P, Ryaboy D, Schmutz J, Schrader J, Segerman B, Shin H, Siddiqui A, Sterky F, Terry A, Tsai CJ, Uberbacher E, Unneberg P, Vahala J, Wall K, Wessler S, Yang G, Yin T, Douglas C, Marra M, Sandberg G, Van de Peer Y, Rokhsar D
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC7459484 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC7459484 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPa424911	XM_024595577.1 Latest version!	Populus trichocarpa chromatin modification-related protein EAF1 B (LOC7459484), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPa424908	XM_024595574.1 Latest version!	Populus trichocarpa chromatin modification-related protein EAF1 B (LOC7459484), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPa424908	XM_024595572.1 Latest version!	Populus trichocarpa chromatin modification-related protein EAF1 B (LOC7459484), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPa424908	XM_024595573.1 Latest version!	Populus trichocarpa chromatin modification-related protein EAF1 B (LOC7459484), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPa424908	XM_024595571.1 Latest version!	Populus trichocarpa chromatin modification-related protein EAF1 B (LOC7459484), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPa424909	XM_024595575.1 Latest version!	Populus trichocarpa chromatin modification-related protein EAF1 B (LOC7459484), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPa424910	XM_024595576.1 Latest version!	Populus trichocarpa chromatin modification-related protein EAF1 B (LOC7459484), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPa04781	XM_002303006.2 Latest version!	Populus trichocarpa hypothetical protein (POPTR_0002s24490g) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC7459484 ( XM_024595577.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024451345.1 Populus trichocarpa chromatin modification-related protein EAF1 B (LOC7459484), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OPa424911
Clone ID Related Accession (Same CDS sequence)	XM_024595577.1
Accession Version	XM_024595577.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	chromatin modification-related protein EAF1 B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037286.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGGAT GTGGTTCAGG ATATGCTCTC CTAGTAAATG CTGAGGTTGA TTCCATGGGT 
GGGGTTGTTG ATGGTGGAGT TGGTATTGCT ACCAAAACCT CTCCGCGGCG AGCAGCAATT 
GAGAAGGCCC AGGTGGAACT TAGACAAGAG TATGATGTTC GTGAGGAACG GAGAAGGGAG 
CTAGAATTTC TTGAGAAAGG CGGCAATCCT TTGGATTTTA AATTTGTAAA TGCAACTTCG 
GTCAGTGTCC AGTCTACTTC ACTCACAGAT CATCACGTTG AACAGTTTGT TACTAGTGAA 
GCAAAAGGCA GTTTTCCACT GACTGCATCA CCTCATGGGG ATTCTGTAGA AAGTAGTGGC 
AGACCAGGGG CTACTCCAGT TTGTGAGCCC AATAGTGCTG ACAATTTTGA TGGTGAAAAT 
GAGTTACTTG AAGTTGAAAG GAAACGTAAA CATCCTAGTA GGAGGAATAA TGTCACTCAA 
TCAGAGCAGT CTTCACAGAT GGATGGAATT CACAATGCCA AGGAATCAGA AGATTCTGCT 
ATTTTTCGGC CATATGCTCG AAGGAATAGG TCCAGACCAA ATCGTGACGG CGCGCGATCA 
AGTTCAACTG ATATTGTTCA GAGTTCAGTT GGCCATGGAT CCTCTTTACC GGCTCGTGGG 
GATGCAAGGG ATGTGAAAGG GTTGGTAACT GAAACAGACG ATCATAAAGC CCAGATTATT 
ACTTCCATCT CTAATCCAAA GTCCACCACT TCAAACGGTG ACCTGTTTTT TCAGATTGAC 
ACTTCCAATA CACAGTCAAA CACGGAGCTT GATTGTGTGC AGGCTCTGAA AACAGTAGTC 
AACCTGCCTG ATGATAGGTT GGATGTTACA GAGTCCATTG TCTTGAGGGA TAACCAACAT 
GACCAGCCTT CAGAAGCTGA TGCTGAGAAA GCACCCAATG ATATTGCTTC CAGGGAATGC 
GATCATGGTG GAGGAAAAGA GCTGGTTATT TCAGCTGGTC CTGAATGTCC CCCTTGTGCT 
GAATCAGCCA AGACTGAGAA TGAAACTGGT CCTGCTCTGC TCAATGGCTT GGAAAAAGAT 
GGAAATGAAG GTCAAAATGG TAATGCAGCA ATGGGAACGG AACGATTCAA TTCAGAGTCC 
TCTTGCACCC AAAATAGCTT AAGCCTGGAT GCAAACAATG GCTGTGATCC TTGTGATAAT 
CGTAGAAATG ATGACACCAA TGAAATTCTT TTGAAAGAAT CATCAGAATT TGAAGGAACA 
CGAAGTTTAC CATCGGGTAA TATAGGAAAT GAAAAGAAAG AGACTAACTC GATCTCTGCA 
ATCAATGATG GTTCTGTGCA TGAAAACTAC TCTGGCAATG ATTCTACTGT CAAAAATGAG 
GAAGAAAGGA GAACAACTTT TCACTCTCAG GTGAAGTGTA CTAATCTTGA AGGAGTGGAA 
CAAAATGACC ATGTTGCCTC TGAGGCTGAT ACAAAGGCGG GCAACATGTT GGCTGATAGT 
TCTAATTCCA TCAGGGAAAT AATTTACCCC AGTGGTCCCC AGGGCTCTTT GGATCCTCCT 
GTTCAAGAGC TTCCCCAGCC TATTTTGTTA GAGAAAAACT CTTTTGTTGC CACTGATCCT 
CAATCTTGTT CGAATACACA TGTTAAAGTG GTAGACAAGT CTCATGAAGA TTCTATTTTG 
GAAGAGGCAC GGGTTATAGA GGCTAAGCGC AAGAGGATTG CAGAATTATC TGTTGCATCT 
GTGCATTCAG AGAATCGCCG GAGATCTCAC TGGGATTTTG TCCTTGAAGA AATGGCATGG 
CTGGCAAATG ATGTTGCACA GGAGCGTCTC TGGAAGATGA CTGCTGCTGC TCAAATATGT 
CGACGAATTG CTTTTACTTC TCGATTGAGA GTTGAAGAGC AGAATCATCA TTTGAAGCTA 
AAAAACGTCG CTTACAGTCT TGCTAAGGCT GTCATGCAGT TCTGGCATTC AGCAAAGGTG 
TATCTAAGTA ACAATTGTCA CAGTGTTGGT TCAAAGAATG GGAAGCATGA GGTGGGGATG 
TTTGTTGGGA ATGAATTTTC TGTCAACAAG TTTGGAGATA TTGATAAGGA GCAGGTGGCA 
TGCAAAGAGT TAGAGAAACA AAATCCTGCC AAAAACATTG CGCATTCCAT TCATGGATAT 
GCTGTAAGGT TTTTGAAATA TAACAGTTCT CCATTTCCTT CCTTTCAAGC AGAAGCACCA 
GCAACTCCTG ATAGAATAGC TGATTTGGGC ATTGTGGACA CTTCATGGGA TGATCGCCTT 
ACTGAAGAAA GCCTGTTCTA CGCAGTTCCC TCAGGTGCCA TGGCAATGTA CAGATTGTCC 
ATTGAATCTC ATATTGCGCA GAGTGAGAAA ACTCGCAGTA GCATGCAGGA GGAAGTCGAT 
ACATCCATGT ATGACACTCC AGCAGATTTT GGATATCATG ATACTGCTGC ATACGATGAG 
GAGGAAGGAG AAACAAGTGC TTACTATATG CATGGAGTCT TTGAAGGTAG CAAGTCAGCA 
AAACATGACC AAAAGAAACG GAAAAGCTTA ACAAAGTCAC CCTCTGCAAG ATCTTATGAT 
CTGGGAACTG ATTCACCTTA TGGACACTGC ACCACTGGGC CACAACAAAA TGTATTGATG 
GGGAAAAGAC CTGCTAGTAA TCTTAATGCT GGCTCAATCC CAACAAAACG CATGCGTACT 
GCTTCTAGAC AGAGGTTTAC AAGTCCTTTT ACTGCTGGAA CTGCTGGGGT TTTACTCCAG 
GCTCCAGTGA AGACAGATGC ATCGAGTGGA GATACCAATT CTTTTCAGGA TGATCAGAGT 
ATTTTGCATG GTGGGTCACA AATTCAGAAA AGTGTGGAGG TTGAGTCCGC TGCGCATTTC 
GAAAGGCAGT TACCATATGA CTACGCAGAG ACATCAACAA AACCGAAGAA GAAGAAGAAG 
GCAAAGCACC TGGGTTCTGC TTATGAGCAG GGGTGGCAGT TGGATTCTAC TGGTCATAAT 
GAACAGAGGG ATAATTTCAA AAAGAGATCG GAGAGTCATC ATTTGGACTC TAATGGAACC 
AGTGGTTTAT ATGGGCAACA TACCACAAAG AAGCCAAAGA TATCTAAGCA ATTGCTAGAT 
AATACTTTTG ACAACATGGT TCAAATGACC GGGTCCATCC CATCTCCAGC TGCTTCCCAG 
ATGAGTAATA TGTCAAACAC AAATAGATTC ATCAAATTGA TTGGTGGCAG GGAACGAGGC 
AGAAAAAATA AATCAATGAA GATGTCTGTT GGGCAGCCAG GTTCTGGAAG TCCCTGGTCA 
CTTTTTGAAG ACCAGGCTCT TGTTGTTCTT GTGCATGACA TGGGTCCTAA TTGGGAGCTT 
ATAAGCGATG CTATCAACAG CACTGCTCAA TTCAAGTGTA TATTCCGCAA GCCTAAAGAA 
TGCAAAGATC GTCACAAAAT CTTAATGGAT AAGGGTGCTG GTGATGGGGC TGATAGTGCT 
GAGGATTCAG GATCTTCTCA ATCCTACCCA TCAACTTTGC CTGGCATTCC AAAGCAGGGC 
AGTGCCAGAC AGTTGTTTCA ACATTTGCAA GGGCCGATGC AAGAGGATAC CCTGAAGTCT 
CATTTTGAGA AAATCATCAT TATTGGGAAG AAACATCATT ACAAAAGGAG TCAGAATGAA 
AACCAGGATC CCAAGCAAAT AGCAGCAACT CACAATTCTC ATTTTATTGC TCTTTCCCAA 
GTTTGCCCAA ACAACTTAAA TGGAGGTGTC CTAACGCCCC TTGATCTCTG TGATTCAAGC 
ACTTCAAATC CAGATGTTCT TCCCAATGTG TATCAAGGTT CTCATGCTAG CAATTTGGTA 
ATGCCAAACC AAGGTGCTGT TGCATCAACG CTTCCCACTT CTGGTGCAAT CTCTTCTCTG 
CAAGGATCTT CTGGCGTGGT TCTTGGCAAT AACTCATCAT CACCATCTGG TCCCCTCAAT 
GCACCTCATA GAGATGGTAG ATACAATGTT CCAAGGACAT CTTTACCAGT TGACGAGCAT 
CAAAGAATGC AACCCTATCA AATGTTACCT AGTAGAAACT TGCAGCAGTC TAACATGTCG 
GTTTCTGGGG CTGTCTCTGG AGCTGATCGT GGTGTTCGCA TGTTATCGAG TGGAAATGGT 
ATGGGCATGA TGCCAGGGAT GAACAGAAGC ATGCCACTGC CACGGTCAGG ATTTCAAGGA 
ACAGCCTCTT CATCAATGCT GAATTCTGGA AGTATGCTTT CTAACAATGT GGTTGGGATG 
TCAAGCCCTG TGAATATGCA CACTGGTTCT GGTCAAGGGA ACTTGATGAG ACCTCGCGAG 
GCTTTGCATA TGTTGAGGAC ATATCCAGGC CATCCCCAGC AGCAGCATCA AATGCCTGCA 
CAACAGTCCA ATATGCTTAG CAATCCTCAT CATCCTAATC TCCGTGGCCC CAATCAGGCT 
ACAGGTTCAC AGCATCCAGC ATATGCTATG CACCTGCCTA AAGGAAGGCA AATGCAGCAG 
CGGCTTCTCC AGCAGCAGCA GCAGCAGCAG CAGCACTTTT CTGCATCTAG TGCTTTGATG 
CCCCATGTCC AACATCAATC CCAACTTCCC ATATCATCGT CTATGCAAAA TAGTTCTCAA 
ATCTCACCAC CATCTGCATC GCAGCCGGTG TCACTACCTG CTATAACTCC CCCTTCCCCA 
ATGACTCCCA TTTCAATGCA GCAGCAACAG CAGCAGAAAC ACAACTTGCC ACATCATGCG 
GTCAGTCGGA GCCCCCAAAG TGGTTCCAGT GGGTTGACCA ATCAGATGGG AAAGCAGCGA 
CAACGGCAAC CACAGCAGTT TCAACAATCT GGCAGACATC ACCCTCAACA ACGACAACAC 
TCACAGTCTC CACAGCAGGC TAAACTTTTG AAGGGAATGG GGAGAGGGAA CATGGTGGTG 
CACCAGAACC TTCCCAATGA TCACTCTCCT TTGAATGGCC TTTCTGTGCC TCCAGGAAAC 
CATGGTGTAG AGAAAGGAGA GCAAATCATG CACTTGATGC AGGGTCAAGG CTTGTATTCT 
GGGACTGGGT TGAGTCCAAT TCATACATCT AAACCATTGG GTCCTTCACA ATCTCCAAAC 
CATTCTCAGC CACAGCAAAA GCTATATTCG GGCCCTACAA CCCCTTCATC AAAGCCACTC 
CAGCAAATGC CTTCTCATTT GGAAAGTAGT ACTCAAGGCC AGGTCCAACC AGTACCCTCT 
GGTCAAACAC TAACAGCTAC TCACCAGAAC ACTCCAGTTA TGGTTCCCAG TCATCAGCAT 
CTTCAACAAC ATCCACAGCC ACATCAGAAG CAGGTTAGTC AACCTCAACC AACTGTTCAG 
AGAATGCTTC AACAGAGTCG TCTGTTGAAT TCTGATCTGC CAACAAAGCC TCAAACTGAT 
CAGGGCCATG CTGATCAGCA AACTTCAAAC AACATCTCCC AGACAGGAAC AAGTACATCC 
ACAGGGATGC CTTTGGCCTG TAATGATACA TCTAATGTGG CACCAGTTGT TTCTTCTGTT 
TCTGAGATGC AGTGGAAATC ATCAGAACCA TCCTTGCATG ATTCTGGCAT GGCAGATACA 
GCTTCTAAGG TAGGTCCTAT TGGTAGCCCC CCCCTTACGA GTGCAGCTGG GAGTGAGCAA 
GTGGTGCCTG TCAGCCAAGG TTCAGTCCAC AGGCAGTTAT CAGGAGGCTT GCCCCTGCAT 
TGCCATAATG GTGGGACACG GTGGCAGCAC CAACAACCAC ATCAATCAAC AGCATCGCCT 
TCCCCATGCC AGCAACTTTT GCAGTCACAA GAGCCACAAC TACAGCAAGA ACGACAACAA 
TCACCTCAAC AGCTGCCACC ACAGCAGCCG ACTCAGAAGC AGACACAGCA TCTTCAATCA 
GGACAAGGCA ATTTATACAT CACGCCAAGC AATTCTGGTC TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024451345.1
CDS	401..6328
Translation	MHGCGSGYALLVNAEVDSMGGVVDGGVGIATKTSPRRAAIEKAQVELRQEYDVREERRRELEFLEKGGNPLDFKFVNATSVSVQSTSLTDHHVEQFVTSEAKGSFPLTASPHGDSVESSGRPGATPVCEPNSADNFDGENELLEVERKRKHPSRRNNVTQSEQSSQMDGIHNAKESEDSAIFRPYARRNRSRPNRDGARSSSTDIVQSSVGHGSSLPARGDARDVKGLVTETDDHKAQIITSISNPKSTTSNGDLFFQIDTSNTQSNTELDCVQALKTVVNLPDDRLDVTESIVLRDNQHDQPSEADAEKAPNDIASRECDHGGGKELVISAGPECPPCAESAKTENETGPALLNGLEKDGNEGQNGNAAMGTERFNSESSCTQNSLSLDANNGCDPCDNRRNDDTNEILLKESSEFEGTRSLPSGNIGNEKKETNSISAINDGSVHENYSGNDSTVKNEEERRTTFHSQVKCTNLEGVEQNDHVASEADTKAGNMLADSSNSIREIIYPSGPQGSLDPPVQELPQPILLEKNSFVATDPQSCSNTHVKVVDKSHEDSILEEARVIEAKRKRIAELSVASVHSENRRRSHWDFVLEEMAWLANDVAQERLWKMTAAAQICRRIAFTSRLRVEEQNHHLKLKNVAYSLAKAVMQFWHSAKVYLSNNCHSVGSKNGKHEVGMFVGNEFSVNKFGDIDKEQVACKELEKQNPAKNIAHSIHGYAVRFLKYNSSPFPSFQAEAPATPDRIADLGIVDTSWDDRLTEESLFYAVPSGAMAMYRLSIESHIAQSEKTRSSMQEEVDTSMYDTPADFGYHDTAAYDEEEGETSAYYMHGVFEGSKSAKHDQKKRKSLTKSPSARSYDLGTDSPYGHCTTGPQQNVLMGKRPASNLNAGSIPTKRMRTASRQRFTSPFTAGTAGVLLQAPVKTDASSGDTNSFQDDQSILHGGSQIQKSVEVESAAHFERQLPYDYAETSTKPKKKKKAKHLGSAYEQGWQLDSTGHNEQRDNFKKRSESHHLDSNGTSGLYGQHTTKKPKISKQLLDNTFDNMVQMTGSIPSPAASQMSNMSNTNRFIKLIGGRERGRKNKSMKMSVGQPGSGSPWSLFEDQALVVLVHDMGPNWELISDAINSTAQFKCIFRKPKECKDRHKILMDKGAGDGADSAEDSGSSQSYPSTLPGIPKQGSARQLFQHLQGPMQEDTLKSHFEKIIIIGKKHHYKRSQNENQDPKQIAATHNSHFIALSQVCPNNLNGGVLTPLDLCDSSTSNPDVLPNVYQGSHASNLVMPNQGAVASTLPTSGAISSLQGSSGVVLGNNSSSPSGPLNAPHRDGRYNVPRTSLPVDEHQRMQPYQMLPSRNLQQSNMSVSGAVSGADRGVRMLSSGNGMGMMPGMNRSMPLPRSGFQGTASSSMLNSGSMLSNNVVGMSSPVNMHTGSGQGNLMRPREALHMLRTYPGHPQQQHQMPAQQSNMLSNPHHPNLRGPNQATGSQHPAYAMHLPKGRQMQQRLLQQQQQQQQHFSASSALMPHVQHQSQLPISSSMQNSSQISPPSASQPVSLPAITPPSPMTPISMQQQQQQKHNLPHHAVSRSPQSGSSGLTNQMGKQRQRQPQQFQQSGRHHPQQRQHSQSPQQAKLLKGMGRGNMVVHQNLPNDHSPLNGLSVPPGNHGVEKGEQIMHLMQGQGLYSGTGLSPIHTSKPLGPSQSPNHSQPQQKLYSGPTTPSSKPLQQMPSHLESSTQGQVQPVPSGQTLTATHQNTPVMVPSHQHLQQHPQPHQKQVSQPQPTVQRMLQQSRLLNSDLPTKPQTDQGHADQQTSNNISQTGTSTSTGMPLACNDTSNVAPVVSSVSEMQWKSSEPSLHDSGMADTASKVGPIGSPPLTSAAGSEQVVPVSQGSVHRQLSGGLPLHCHNGGTRWQHQQPHQSTASPSPCQQLLQSQEPQLQQERQQSPQQLPPQQPTQKQTQHLQSGQGNLYITPSNSGLE

Target ORF information:

RefSeq Version	XM_024595577.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa chromatin modification-related protein EAF1 B (LOC7459484), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024595577.1

ORF Insert Sequence:

ATGCATGGAT GTGGTTCAGG ATATGCTCTC CTAGTAAATG CTGAGGTTGA TTCCATGGGT 
GGGGTTGTTG ATGGTGGAGT TGGTATTGCT ACCAAAACCT CTCCGCGGCG AGCAGCAATT 
GAGAAGGCCC AGGTGGAACT TAGACAAGAG TATGATGTTC GTGAGGAACG GAGAAGGGAG 
CTAGAATTTC TTGAGAAAGG CGGCAATCCT TTGGATTTTA AATTTGTAAA TGCAACTTCG 
GTCAGTGTCC AGTCTACTTC ACTCACAGAT CATCACGTTG AACAGTTTGT TACTAGTGAA 
GCAAAAGGCA GTTTTCCACT GACTGCATCA CCTCATGGGG ATTCTGTAGA AAGTAGTGGC 
AGACCAGGGG CTACTCCAGT TTGTGAGCCC AATAGTGCTG ACAATTTTGA TGGTGAAAAT 
GAGTTACTTG AAGTTGAAAG GAAACGTAAA CATCCTAGTA GGAGGAATAA TGTCACTCAA 
TCAGAGCAGT CTTCACAGAT GGATGGAATT CACAATGCCA AGGAATCAGA AGATTCTGCT 
ATTTTTCGGC CATATGCTCG AAGGAATAGG TCCAGACCAA ATCGTGACGG CGCGCGATCA 
AGTTCAACTG ATATTGTTCA GAGTTCAGTT GGCCATGGAT CCTCTTTACC GGCTCGTGGG 
GATGCAAGGG ATGTGAAAGG GTTGGTAACT GAAACAGACG ATCATAAAGC CCAGATTATT 
ACTTCCATCT CTAATCCAAA GTCCACCACT TCAAACGGTG ACCTGTTTTT TCAGATTGAC 
ACTTCCAATA CACAGTCAAA CACGGAGCTT GATTGTGTGC AGGCTCTGAA AACAGTAGTC 
AACCTGCCTG ATGATAGGTT GGATGTTACA GAGTCCATTG TCTTGAGGGA TAACCAACAT 
GACCAGCCTT CAGAAGCTGA TGCTGAGAAA GCACCCAATG ATATTGCTTC CAGGGAATGC 
GATCATGGTG GAGGAAAAGA GCTGGTTATT TCAGCTGGTC CTGAATGTCC CCCTTGTGCT 
GAATCAGCCA AGACTGAGAA TGAAACTGGT CCTGCTCTGC TCAATGGCTT GGAAAAAGAT 
GGAAATGAAG GTCAAAATGG TAATGCAGCA ATGGGAACGG AACGATTCAA TTCAGAGTCC 
TCTTGCACCC AAAATAGCTT AAGCCTGGAT GCAAACAATG GCTGTGATCC TTGTGATAAT 
CGTAGAAATG ATGACACCAA TGAAATTCTT TTGAAAGAAT CATCAGAATT TGAAGGAACA 
CGAAGTTTAC CATCGGGTAA TATAGGAAAT GAAAAGAAAG AGACTAACTC GATCTCTGCA 
ATCAATGATG GTTCTGTGCA TGAAAACTAC TCTGGCAATG ATTCTACTGT CAAAAATGAG 
GAAGAAAGGA GAACAACTTT TCACTCTCAG GTGAAGTGTA CTAATCTTGA AGGAGTGGAA 
CAAAATGACC ATGTTGCCTC TGAGGCTGAT ACAAAGGCGG GCAACATGTT GGCTGATAGT 
TCTAATTCCA TCAGGGAAAT AATTTACCCC AGTGGTCCCC AGGGCTCTTT GGATCCTCCT 
GTTCAAGAGC TTCCCCAGCC TATTTTGTTA GAGAAAAACT CTTTTGTTGC CACTGATCCT 
CAATCTTGTT CGAATACACA TGTTAAAGTG GTAGACAAGT CTCATGAAGA TTCTATTTTG 
GAAGAGGCAC GGGTTATAGA GGCTAAGCGC AAGAGGATTG CAGAATTATC TGTTGCATCT 
GTGCATTCAG AGAATCGCCG GAGATCTCAC TGGGATTTTG TCCTTGAAGA AATGGCATGG 
CTGGCAAATG ATGTTGCACA GGAGCGTCTC TGGAAGATGA CTGCTGCTGC TCAAATATGT 
CGACGAATTG CTTTTACTTC TCGATTGAGA GTTGAAGAGC AGAATCATCA TTTGAAGCTA 
AAAAACGTCG CTTACAGTCT TGCTAAGGCT GTCATGCAGT TCTGGCATTC AGCAAAGGTG 
TATCTAAGTA ACAATTGTCA CAGTGTTGGT TCAAAGAATG GGAAGCATGA GGTGGGGATG 
TTTGTTGGGA ATGAATTTTC TGTCAACAAG TTTGGAGATA TTGATAAGGA GCAGGTGGCA 
TGCAAAGAGT TAGAGAAACA AAATCCTGCC AAAAACATTG CGCATTCCAT TCATGGATAT 
GCTGTAAGGT TTTTGAAATA TAACAGTTCT CCATTTCCTT CCTTTCAAGC AGAAGCACCA 
GCAACTCCTG ATAGAATAGC TGATTTGGGC ATTGTGGACA CTTCATGGGA TGATCGCCTT 
ACTGAAGAAA GCCTGTTCTA CGCAGTTCCC TCAGGTGCCA TGGCAATGTA CAGATTGTCC 
ATTGAATCTC ATATTGCGCA GAGTGAGAAA ACTCGCAGTA GCATGCAGGA GGAAGTCGAT 
ACATCCATGT ATGACACTCC AGCAGATTTT GGATATCATG ATACTGCTGC ATACGATGAG 
GAGGAAGGAG AAACAAGTGC TTACTATATG CATGGAGTCT TTGAAGGTAG CAAGTCAGCA 
AAACATGACC AAAAGAAACG GAAAAGCTTA ACAAAGTCAC CCTCTGCAAG ATCTTATGAT 
CTGGGAACTG ATTCACCTTA TGGACACTGC ACCACTGGGC CACAACAAAA TGTATTGATG 
GGGAAAAGAC CTGCTAGTAA TCTTAATGCT GGCTCAATCC CAACAAAACG CATGCGTACT 
GCTTCTAGAC AGAGGTTTAC AAGTCCTTTT ACTGCTGGAA CTGCTGGGGT TTTACTCCAG 
GCTCCAGTGA AGACAGATGC ATCGAGTGGA GATACCAATT CTTTTCAGGA TGATCAGAGT 
ATTTTGCATG GTGGGTCACA AATTCAGAAA AGTGTGGAGG TTGAGTCCGC TGCGCATTTC 
GAAAGGCAGT TACCATATGA CTACGCAGAG ACATCAACAA AACCGAAGAA GAAGAAGAAG 
GCAAAGCACC TGGGTTCTGC TTATGAGCAG GGGTGGCAGT TGGATTCTAC TGGTCATAAT 
GAACAGAGGG ATAATTTCAA AAAGAGATCG GAGAGTCATC ATTTGGACTC TAATGGAACC 
AGTGGTTTAT ATGGGCAACA TACCACAAAG AAGCCAAAGA TATCTAAGCA ATTGCTAGAT 
AATACTTTTG ACAACATGGT TCAAATGACC GGGTCCATCC CATCTCCAGC TGCTTCCCAG 
ATGAGTAATA TGTCAAACAC AAATAGATTC ATCAAATTGA TTGGTGGCAG GGAACGAGGC 
AGAAAAAATA AATCAATGAA GATGTCTGTT GGGCAGCCAG GTTCTGGAAG TCCCTGGTCA 
CTTTTTGAAG ACCAGGCTCT TGTTGTTCTT GTGCATGACA TGGGTCCTAA TTGGGAGCTT 
ATAAGCGATG CTATCAACAG CACTGCTCAA TTCAAGTGTA TATTCCGCAA GCCTAAAGAA 
TGCAAAGATC GTCACAAAAT CTTAATGGAT AAGGGTGCTG GTGATGGGGC TGATAGTGCT 
GAGGATTCAG GATCTTCTCA ATCCTACCCA TCAACTTTGC CTGGCATTCC AAAGCAGGGC 
AGTGCCAGAC AGTTGTTTCA ACATTTGCAA GGGCCGATGC AAGAGGATAC CCTGAAGTCT 
CATTTTGAGA AAATCATCAT TATTGGGAAG AAACATCATT ACAAAAGGAG TCAGAATGAA 
AACCAGGATC CCAAGCAAAT AGCAGCAACT CACAATTCTC ATTTTATTGC TCTTTCCCAA 
GTTTGCCCAA ACAACTTAAA TGGAGGTGTC CTAACGCCCC TTGATCTCTG TGATTCAAGC 
ACTTCAAATC CAGATGTTCT TCCCAATGTG TATCAAGGTT CTCATGCTAG CAATTTGGTA 
ATGCCAAACC AAGGTGCTGT TGCATCAACG CTTCCCACTT CTGGTGCAAT CTCTTCTCTG 
CAAGGATCTT CTGGCGTGGT TCTTGGCAAT AACTCATCAT CACCATCTGG TCCCCTCAAT 
GCACCTCATA GAGATGGTAG ATACAATGTT CCAAGGACAT CTTTACCAGT TGACGAGCAT 
CAAAGAATGC AACCCTATCA AATGTTACCT AGTAGAAACT TGCAGCAGTC TAACATGTCG 
GTTTCTGGGG CTGTCTCTGG AGCTGATCGT GGTGTTCGCA TGTTATCGAG TGGAAATGGT 
ATGGGCATGA TGCCAGGGAT GAACAGAAGC ATGCCACTGC CACGGTCAGG ATTTCAAGGA 
ACAGCCTCTT CATCAATGCT GAATTCTGGA AGTATGCTTT CTAACAATGT GGTTGGGATG 
TCAAGCCCTG TGAATATGCA CACTGGTTCT GGTCAAGGGA ACTTGATGAG ACCTCGCGAG 
GCTTTGCATA TGTTGAGGAC ATATCCAGGC CATCCCCAGC AGCAGCATCA AATGCCTGCA 
CAACAGTCCA ATATGCTTAG CAATCCTCAT CATCCTAATC TCCGTGGCCC CAATCAGGCT 
ACAGGTTCAC AGCATCCAGC ATATGCTATG CACCTGCCTA AAGGAAGGCA AATGCAGCAG 
CGGCTTCTCC AGCAGCAGCA GCAGCAGCAG CAGCACTTTT CTGCATCTAG TGCTTTGATG 
CCCCATGTCC AACATCAATC CCAACTTCCC ATATCATCGT CTATGCAAAA TAGTTCTCAA 
ATCTCACCAC CATCTGCATC GCAGCCGGTG TCACTACCTG CTATAACTCC CCCTTCCCCA 
ATGACTCCCA TTTCAATGCA GCAGCAACAG CAGCAGAAAC ACAACTTGCC ACATCATGCG 
GTCAGTCGGA GCCCCCAAAG TGGTTCCAGT GGGTTGACCA ATCAGATGGG AAAGCAGCGA 
CAACGGCAAC CACAGCAGTT TCAACAATCT GGCAGACATC ACCCTCAACA ACGACAACAC 
TCACAGTCTC CACAGCAGGC TAAACTTTTG AAGGGAATGG GGAGAGGGAA CATGGTGGTG 
CACCAGAACC TTCCCAATGA TCACTCTCCT TTGAATGGCC TTTCTGTGCC TCCAGGAAAC 
CATGGTGTAG AGAAAGGAGA GCAAATCATG CACTTGATGC AGGGTCAAGG CTTGTATTCT 
GGGACTGGGT TGAGTCCAAT TCATACATCT AAACCATTGG GTCCTTCACA ATCTCCAAAC 
CATTCTCAGC CACAGCAAAA GCTATATTCG GGCCCTACAA CCCCTTCATC AAAGCCACTC 
CAGCAAATGC CTTCTCATTT GGAAAGTAGT ACTCAAGGCC AGGTCCAACC AGTACCCTCT 
GGTCAAACAC TAACAGCTAC TCACCAGAAC ACTCCAGTTA TGGTTCCCAG TCATCAGCAT 
CTTCAACAAC ATCCACAGCC ACATCAGAAG CAGGTTAGTC AACCTCAACC AACTGTTCAG 
AGAATGCTTC AACAGAGTCG TCTGTTGAAT TCTGATCTGC CAACAAAGCC TCAAACTGAT 
CAGGGCCATG CTGATCAGCA AACTTCAAAC AACATCTCCC AGACAGGAAC AAGTACATCC 
ACAGGGATGC CTTTGGCCTG TAATGATACA TCTAATGTGG CACCAGTTGT TTCTTCTGTT 
TCTGAGATGC AGTGGAAATC ATCAGAACCA TCCTTGCATG ATTCTGGCAT GGCAGATACA 
GCTTCTAAGG TAGGTCCTAT TGGTAGCCCC CCCCTTACGA GTGCAGCTGG GAGTGAGCAA 
GTGGTGCCTG TCAGCCAAGG TTCAGTCCAC AGGCAGTTAT CAGGAGGCTT GCCCCTGCAT 
TGCCATAATG GTGGGACACG GTGGCAGCAC CAACAACCAC ATCAATCAAC AGCATCGCCT 
TCCCCATGCC AGCAACTTTT GCAGTCACAA GAGCCACAAC TACAGCAAGA ACGACAACAA 
TCACCTCAAC AGCTGCCACC ACAGCAGCCG ACTCAGAAGC AGACACAGCA TCTTCAATCA 
GGACAAGGCA ATTTATACAT CACGCCAAGC AATTCTGGTC TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7459484 ( XM_024595574.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024451342.1 Populus trichocarpa chromatin modification-related protein EAF1 B (LOC7459484), transcript variant X4, mRNA.

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CloneID	OPa424908
Clone ID Related Accession (Same CDS sequence)	XM_024595574.1 , XM_024595573.1 , XM_024595571.1 , XM_024595572.1
Accession Version	XM_024595574.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	chromatin modification-related protein EAF1 B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037286.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGGAT GTGGTTCAGG ATATGCTCTC CTAGTAAATG CTGAGGTTGA TTCCATGGGT 
GGGGTTGTTG ATGGTGGAGT TGGTATTGCT ACCAAAACCT CTCCGCGGCG AGCAGCAATT 
GAGAAGGCCC AGGTGGAACT TAGACAAGAG TATGATGTTC GTGAGGAACG GAGAAGGGAG 
CTAGAATTTC TTGAGAAAGG CGGCAATCCT TTGGATTTTA AATTTGTAAA TGCAACTTCG 
GTCAGTGTCC AGTCTACTTC ACTCACAGAT CATCACGTTG AACAGTTTGT TACTAGTGAA 
GCAAAAGGCA GTTTTCCACT GACTGCATCA CCTCATGGGG ATTCTGTAGA AAGTAGTGGC 
AGACCAGGGG CTACTCCAGT TTGTGAGCCC AATAGTGCTG ACAATTTTGA TGGTGAAAAT 
GAGTTACTTG AAGTTGAAAG GAAACGTAAA CATCCTAGTA GGAGGAATAA TGTCACTCAA 
TCAGAGCAGT CTTCACAGAT GGATGGAATT CACAATGCCA AGGAATCAGA AGATTCTGCT 
ATTTTTCGGC CATATGCTCG AAGGAATAGG TCCAGACCAA ATCGTGACGG CGCGCGATCA 
AGTTCAACTG ATATTGTTCA GAGTTCAGTT GGCCATGGAT CCTCTTTACC GGCTCGTGGG 
GATGCAAGGG ATGTGAAAGG GTTGGTAACT GAAACAGACG ATCATAAAGC CCAGATTATT 
ACTTCCATCT CTAATCCAAA GTCCACCACT TCAAACGGTG ACCTGTTTTT TCAGATTGAC 
ACTTCCAATA CACAGTCAAA CACGGAGCTT GATTGTGTGC AGGCTCTGAA AACAGTAGTC 
AACCTGCCTG ATGATAGGTT GGATGTTACA GAGTCCATTG TCTTGAGGGA TAACCAACAT 
GACCAGCCTT CAGAAGCTGA TGCTGAGAAA GCACCCAATG ATATTGCTTC CAGGGAATGC 
GATCATGGTG GAGGAAAAGA GCTGGTTATT TCAGCTGGTC CTGAATGTCC CCCTTGTGCT 
GAATCAGCCA AGACTGAGAA TGAAACTGGT CCTGCTCTGC TCAATGGCTT GGAAAAAGAT 
GGAAATGAAG GTCAAAATGG TAATGCAGCA ATGGGAACGG AACGATTCAA TTCAGAGTCC 
TCTTGCACCC AAAATAGCTT AAGCCTGGAT GCAAACAATG GCTGTGATCC TTGTGATAAT 
CGTAGAAATG ATGACACCAA TGAAATTCTT TTGAAAGAAT CATCAGAATT TGAAGGAACA 
CGAAGTTTAC CATCGGGTAA TATAGGAAAT GAAAAGAAAG AGACTAACTC GATCTCTGCA 
ATCAATGATG GTTCTGTGCA TGAAAACTAC TCTGGCAATG ATTCTACTGT CAAAAATGAG 
GAAGAAAGGA GAACAACTTT TCACTCTCAG GTGAAGTGTA CTAATCTTGA AGGAGTGGAA 
CAAAATGACC ATGTTGCCTC TGAGGCTGAT ACAAAGGCGG GCAACATGTT GGCTGATAGT 
TCTAATTCCA TCAGGGAAAT AATTTACCCC AGTGGTCCCC AGGGCTCTTT GGATCCTCCT 
GTTCAAGAGC TTCCCCAGCC TATTTTGTTA GAGAAAAACT CTTTTGTTGC CACTGATCCT 
CAATCTTGTT CGAATACACA TGTTAAAGTG GTAGACAAGT CTCATGAAGA TTCTATTTTG 
GAAGAGGCAC GGGTTATAGA GGCTAAGCGC AAGAGGATTG CAGAATTATC TGTTGCATCT 
GTGCATTCAG AGAATCGCCG GAGATCTCAC TGGGATTTTG TCCTTGAAGA AATGGCATGG 
CTGGCAAATG ATGTTGCACA GGAGCGTCTC TGGAAGATGA CTGCTGCTGC TCAAATATGT 
CGACGAATTG CTTTTACTTC TCGATTGAGA GTTGAAGAGC AGAATCATCA TTTGAAGCTA 
AAAAACGTCG CTTACAGTCT TGCTAAGGCT GTCATGCAGT TCTGGCATTC AGCAAAGGTG 
TATCTAAGTA ACAATTGTCA CAGTGTTGGT TCAAAGAATG GGAAGCATGA GGTGGGGATG 
TTTGTTGGGA ATGAATTTTC TGTCAACAAG TTTGGAGATA TTGATAAGGA GCAGGTGGCA 
TGCAAAGAGT TAGAGAAACA AAATCCTGCC AAAAACATTG CGCATTCCAT TCATGGATAT 
GCTGTAAGGT TTTTGAAATA TAACAGTTCT CCATTTCCTT CCTTTCAAGC AGAAGCACCA 
GCAACTCCTG ATAGAATAGC TGATTTGGGC ATTGTGGACA CTTCATGGGA TGATCGCCTT 
ACTGAAGAAA GCCTGTTCTA CGCAGTTCCC TCAGGTGCCA TGGCAATGTA CAGATTGTCC 
ATTGAATCTC ATATTGCGCA GAGTGAGAAA ACTCGCAGTA GCATGCAGGA GGAAGTCGAT 
ACATCCATGT ATGACACTCC AGCAGATTTT GGATATCATG ATACTGCTGC ATACGATGAG 
GAGGAAGGAG AAACAAGTGC TTACTATATG CATGGAGTCT TTGAAGGTAG CAAGTCAGCA 
AAACATGACC AAAAGAAACG GAAAAGCTTA ACAAAGTCAC CCTCTGCAAG ATCTTATGAT 
CTGGGAACTG ATTCACCTTA TGGACACTGC ACCACTGGGC CACAACAAAA TGTATTGATG 
GGGAAAAGAC CTGCTAGTAA TCTTAATGCT GGCTCAATCC CAACAAAACG CATGCGTACT 
GCTTCTAGAC AGAGGTTTAC AAGTCCTTTT ACTGCTGGAA CTGCTGGGGT TTTACTCCAG 
GCTCCAGTGA AGACAGATGC ATCGAGTGGA GATACCAATT CTTTTCAGGA TGATCAGAGT 
ATTTTGCATG GTGGGTCACA AATTCAGAAA AGTGTGGAGG TTGAGTCCGC TGCGCATTTC 
GAAAGGCAGT TACCATATGA CTACGCAGAG ACATCAACAA AACCGAAGAA GAAGAAGAAG 
GCAAAGCACC TGGGTTCTGC TTATGAGCAG GGGTGGCAGT TGGATTCTAC TGGTCATAAT 
GAACAGAGGG ATAATTTCAA AAAGAGATCG GAGAGTCATC ATTTGGACTC TAATGGAACC 
AGTGGTTTAT ATGGGCAACA TACCACAAAG AAGCCAAAGA TATCTAAGCA ATTGCTAGAT 
AATACTTTTG ACAACATGGT TCAAATGACC GGGTCCATCC CATCTCCAGC TGCTTCCCAG 
ATGAGTAATA TGTCAAACAC AAATAGATTC ATCAAATTGA TTGGTGGCAG GGAACGAGGC 
AGAAAAAATA AATCAATGAA GATGTCTGTT GGGCAGCCAG GTTCTGGAAG TCCCTGGTCA 
CTTTTTGAAG ACCAGGCTCT TGTTGTTCTT GTGCATGACA TGGGTCCTAA TTGGGAGCTT 
ATAAGCGATG CTATCAACAG CACTGCTCAA TTCAAGTGTA TATTCCGCAA GCCTAAAGAA 
TGCAAAGATC GTCACAAAAT CTTAATGGAT AAGGGTGCTG GTGATGGGGC TGATAGTGCT 
GAGGATTCAG GATCTTCTCA ATCCTACCCA TCAACTTTGC CTGGCATTCC AAAGCAGGGC 
AGTGCCAGAC AGTTGTTTCA ACATTTGCAA GGGCCGATGC AAGAGGATAC CCTGAAGTCT 
CATTTTGAGA AAATCATCAT TATTGGGAAG AAACATCATT ACAAAAGGAG TCAGAATGAA 
AACCAGGATC CCAAGCAAAT AGCAGCAACT CACAATTCTC ATTTTATTGC TCTTTCCCAA 
GTTTGCCCAA ACAACTTAAA TGGAGGTGTC CTAACGCCCC TTGATCTCTG TGATTCAAGC 
ACTTCAAATC CAGATGTTCT TCCCAATGTG TATCAAGGTT CTCATGCTAG CAATTTGGTA 
ATGCCAAACC AAGGTGCTGT TGCATCAACG CTTCCCACTT CTGGTGCAAT CTCTTCTCTG 
CAAGGATCTT CTGGCGTGGT TCTTGGCAAT AACTCATCAT CACCATCTGG TCCCCTCAAT 
GCACCTCATA GAGATGGTAG ATACAATGTT CCAAGGACAT CTTTACCAGT TGACGAGCAT 
CAAAGAATGC AACCCTATCA AATGTTACCT AGTAGAAACT TGCAGCAGTC TAACATGTCG 
GTTTCTGGGG CTGTCTCTGG AGCTGATCGT GGTGTTCGCA TGTTATCGAG TGGAAATGGT 
ATGGGCATGA TGCCAGGGAT GAACAGAAGC ATGCCACTGC CACGGTCAGG ATTTCAAGGA 
ACAGCCTCTT CATCAATGCT GAATTCTGGA AGTATGCTTT CTAACAATGT GGTTGGGATG 
TCAAGCCCTG TGAATATGCA CACTGGTTCT GGTCAAGGGA ACTTGATGAG ACCTCGCGAG 
GCTTTGCATA TGTTGAGGCT TGGCCATAAT CATGAGCATC AAAGACAAAT GATGGTACCA 
GAGCTTCAGA TGCAGCCAAC ACAAGGGAAC AACCAAGGAA TTTCTGCTTT CAACGGGGTG 
CCTACTGCTT TTGCTAATCA GACAACTACT TCACCTGTGC AGACATATCC AGGCCATCCC 
CAGCAGCAGC ATCAAATGCC TGCACAACAG TCCAATATGC TTAGCAATCC TCATCATCCT 
AATCTCCGTG GCCCCAATCA GGCTACAGGT TCACAGCATC CAGCATATGC TATGCACCTG 
CCTAAAGGAA GGCAAATGCA GCAGCGGCTT CTCCAGCAGC AGCAGCAGCA GCAGCAGCAC 
TTTTCTGCAT CTAGTGCTTT GATGCCCCAT GTCCAACATC AATCCCAACT TCCCATATCA 
TCGTCTATGC AAAATAGTTC TCAAATCTCA CCACCATCTG CATCGCAGCC GGTGTCACTA 
CCTGCTATAA CTCCCCCTTC CCCAATGACT CCCATTTCAA TGCAGCAGCA ACAGCAGCAG 
AAACACAACT TGCCACATCA TGCGGTCAGT CGGAGCCCCC AAAGTGGTTC CAGTGGGTTG 
ACCAATCAGA TGGGAAAGCA GCGACAACGG CAACCACAGC AGTTTCAACA ATCTGGCAGA 
CATCACCCTC AACAACGACA ACACTCACAG TCTCCACAGC AGGCTAAACT TTTGAAGGGA 
ATGGGGAGAG GGAACATGGT GGTGCACCAG AACCTTCCCA ATGATCACTC TCCTTTGAAT 
GGCCTTTCTG TGCCTCCAGG AAACCATGGT GTAGAGAAAG GAGAGCAAAT CATGCACTTG 
ATGCAGGGTC AAGGCTTGTA TTCTGGGACT GGGTTGAGTC CAATTCATAC ATCTAAACCA 
TTGGGTCCTT CACAATCTCC AAACCATTCT CAGCCACAGC AAAAGCTATA TTCGGGCCCT 
ACAACCCCTT CATCAAAGCC ACTCCAGCAA ATGCCTTCTC ATTTGGAAAG TAGTACTCAA 
GGCCAGGTCC AACCAGTACC CTCTGGTCAA ACACTAACAG CTACTCACCA GAACACTCCA 
GTTATGGTTC CCAGTCATCA GCATCTTCAA CAACATCCAC AGCCACATCA GAAGCAGGTT 
AGTCAACCTC AACCAACTGT TCAGAGAATG CTTCAACAGA GTCGTCTGTT GAATTCTGAT 
CTGCCAACAA AGCCTCAAAC TGATCAGGGC CATGCTGATC AGCAAACTTC AAACAACATC 
TCCCAGACAG GAACAAGTAC ATCCACAGGG ATGCCTTTGG CCTGTAATGA TACATCTAAT 
GTGGCACCAG TTGTTTCTTC TGTTTCTGAG ATGCAGTGGA AATCATCAGA ACCATCCTTG 
CATGATTCTG GCATGGCAGA TACAGCTTCT AAGGTAGGTC CTATTGGTAG CCCCCCCCTT 
ACGAGTGCAG CTGGGAGTGA GCAAGTGGTG CCTGTCAGCC AAGGTTCAGT CCACAGGCAG 
TTATCAGGAG GCTTGCCCCT GCATTGCCAT AATGGTGGGA CACGGTGGCA GCACCAACAA 
CCACATCAAT CAACAGCATC GCCTTCCCCA TGCCAGCAAC TTTTGCAGTC ACAAGAGCCA 
CAACTACAGC AAGAACGACA ACAATCACCT CAACAGCTGC CACCACAGCA GCCGACTCAG 
AAGCAGACAC AGCATCTTCA ATCAGGACAA GGCAATTTAT ACATCACGCC AAGCAATTCT 
GGTCTGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024451342.1
CDS	391..6462
Translation	MHGCGSGYALLVNAEVDSMGGVVDGGVGIATKTSPRRAAIEKAQVELRQEYDVREERRRELEFLEKGGNPLDFKFVNATSVSVQSTSLTDHHVEQFVTSEAKGSFPLTASPHGDSVESSGRPGATPVCEPNSADNFDGENELLEVERKRKHPSRRNNVTQSEQSSQMDGIHNAKESEDSAIFRPYARRNRSRPNRDGARSSSTDIVQSSVGHGSSLPARGDARDVKGLVTETDDHKAQIITSISNPKSTTSNGDLFFQIDTSNTQSNTELDCVQALKTVVNLPDDRLDVTESIVLRDNQHDQPSEADAEKAPNDIASRECDHGGGKELVISAGPECPPCAESAKTENETGPALLNGLEKDGNEGQNGNAAMGTERFNSESSCTQNSLSLDANNGCDPCDNRRNDDTNEILLKESSEFEGTRSLPSGNIGNEKKETNSISAINDGSVHENYSGNDSTVKNEEERRTTFHSQVKCTNLEGVEQNDHVASEADTKAGNMLADSSNSIREIIYPSGPQGSLDPPVQELPQPILLEKNSFVATDPQSCSNTHVKVVDKSHEDSILEEARVIEAKRKRIAELSVASVHSENRRRSHWDFVLEEMAWLANDVAQERLWKMTAAAQICRRIAFTSRLRVEEQNHHLKLKNVAYSLAKAVMQFWHSAKVYLSNNCHSVGSKNGKHEVGMFVGNEFSVNKFGDIDKEQVACKELEKQNPAKNIAHSIHGYAVRFLKYNSSPFPSFQAEAPATPDRIADLGIVDTSWDDRLTEESLFYAVPSGAMAMYRLSIESHIAQSEKTRSSMQEEVDTSMYDTPADFGYHDTAAYDEEEGETSAYYMHGVFEGSKSAKHDQKKRKSLTKSPSARSYDLGTDSPYGHCTTGPQQNVLMGKRPASNLNAGSIPTKRMRTASRQRFTSPFTAGTAGVLLQAPVKTDASSGDTNSFQDDQSILHGGSQIQKSVEVESAAHFERQLPYDYAETSTKPKKKKKAKHLGSAYEQGWQLDSTGHNEQRDNFKKRSESHHLDSNGTSGLYGQHTTKKPKISKQLLDNTFDNMVQMTGSIPSPAASQMSNMSNTNRFIKLIGGRERGRKNKSMKMSVGQPGSGSPWSLFEDQALVVLVHDMGPNWELISDAINSTAQFKCIFRKPKECKDRHKILMDKGAGDGADSAEDSGSSQSYPSTLPGIPKQGSARQLFQHLQGPMQEDTLKSHFEKIIIIGKKHHYKRSQNENQDPKQIAATHNSHFIALSQVCPNNLNGGVLTPLDLCDSSTSNPDVLPNVYQGSHASNLVMPNQGAVASTLPTSGAISSLQGSSGVVLGNNSSSPSGPLNAPHRDGRYNVPRTSLPVDEHQRMQPYQMLPSRNLQQSNMSVSGAVSGADRGVRMLSSGNGMGMMPGMNRSMPLPRSGFQGTASSSMLNSGSMLSNNVVGMSSPVNMHTGSGQGNLMRPREALHMLRLGHNHEHQRQMMVPELQMQPTQGNNQGISAFNGVPTAFANQTTTSPVQTYPGHPQQQHQMPAQQSNMLSNPHHPNLRGPNQATGSQHPAYAMHLPKGRQMQQRLLQQQQQQQQHFSASSALMPHVQHQSQLPISSSMQNSSQISPPSASQPVSLPAITPPSPMTPISMQQQQQQKHNLPHHAVSRSPQSGSSGLTNQMGKQRQRQPQQFQQSGRHHPQQRQHSQSPQQAKLLKGMGRGNMVVHQNLPNDHSPLNGLSVPPGNHGVEKGEQIMHLMQGQGLYSGTGLSPIHTSKPLGPSQSPNHSQPQQKLYSGPTTPSSKPLQQMPSHLESSTQGQVQPVPSGQTLTATHQNTPVMVPSHQHLQQHPQPHQKQVSQPQPTVQRMLQQSRLLNSDLPTKPQTDQGHADQQTSNNISQTGTSTSTGMPLACNDTSNVAPVVSSVSEMQWKSSEPSLHDSGMADTASKVGPIGSPPLTSAAGSEQVVPVSQGSVHRQLSGGLPLHCHNGGTRWQHQQPHQSTASPSPCQQLLQSQEPQLQQERQQSPQQLPPQQPTQKQTQHLQSGQGNLYITPSNSGLE

Target ORF information:

RefSeq Version	XM_024595574.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa chromatin modification-related protein EAF1 B (LOC7459484), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024595574.1

ORF Insert Sequence:

ATGCATGGAT GTGGTTCAGG ATATGCTCTC CTAGTAAATG CTGAGGTTGA TTCCATGGGT 
GGGGTTGTTG ATGGTGGAGT TGGTATTGCT ACCAAAACCT CTCCGCGGCG AGCAGCAATT 
GAGAAGGCCC AGGTGGAACT TAGACAAGAG TATGATGTTC GTGAGGAACG GAGAAGGGAG 
CTAGAATTTC TTGAGAAAGG CGGCAATCCT TTGGATTTTA AATTTGTAAA TGCAACTTCG 
GTCAGTGTCC AGTCTACTTC ACTCACAGAT CATCACGTTG AACAGTTTGT TACTAGTGAA 
GCAAAAGGCA GTTTTCCACT GACTGCATCA CCTCATGGGG ATTCTGTAGA AAGTAGTGGC 
AGACCAGGGG CTACTCCAGT TTGTGAGCCC AATAGTGCTG ACAATTTTGA TGGTGAAAAT 
GAGTTACTTG AAGTTGAAAG GAAACGTAAA CATCCTAGTA GGAGGAATAA TGTCACTCAA 
TCAGAGCAGT CTTCACAGAT GGATGGAATT CACAATGCCA AGGAATCAGA AGATTCTGCT 
ATTTTTCGGC CATATGCTCG AAGGAATAGG TCCAGACCAA ATCGTGACGG CGCGCGATCA 
AGTTCAACTG ATATTGTTCA GAGTTCAGTT GGCCATGGAT CCTCTTTACC GGCTCGTGGG 
GATGCAAGGG ATGTGAAAGG GTTGGTAACT GAAACAGACG ATCATAAAGC CCAGATTATT 
ACTTCCATCT CTAATCCAAA GTCCACCACT TCAAACGGTG ACCTGTTTTT TCAGATTGAC 
ACTTCCAATA CACAGTCAAA CACGGAGCTT GATTGTGTGC AGGCTCTGAA AACAGTAGTC 
AACCTGCCTG ATGATAGGTT GGATGTTACA GAGTCCATTG TCTTGAGGGA TAACCAACAT 
GACCAGCCTT CAGAAGCTGA TGCTGAGAAA GCACCCAATG ATATTGCTTC CAGGGAATGC 
GATCATGGTG GAGGAAAAGA GCTGGTTATT TCAGCTGGTC CTGAATGTCC CCCTTGTGCT 
GAATCAGCCA AGACTGAGAA TGAAACTGGT CCTGCTCTGC TCAATGGCTT GGAAAAAGAT 
GGAAATGAAG GTCAAAATGG TAATGCAGCA ATGGGAACGG AACGATTCAA TTCAGAGTCC 
TCTTGCACCC AAAATAGCTT AAGCCTGGAT GCAAACAATG GCTGTGATCC TTGTGATAAT 
CGTAGAAATG ATGACACCAA TGAAATTCTT TTGAAAGAAT CATCAGAATT TGAAGGAACA 
CGAAGTTTAC CATCGGGTAA TATAGGAAAT GAAAAGAAAG AGACTAACTC GATCTCTGCA 
ATCAATGATG GTTCTGTGCA TGAAAACTAC TCTGGCAATG ATTCTACTGT CAAAAATGAG 
GAAGAAAGGA GAACAACTTT TCACTCTCAG GTGAAGTGTA CTAATCTTGA AGGAGTGGAA 
CAAAATGACC ATGTTGCCTC TGAGGCTGAT ACAAAGGCGG GCAACATGTT GGCTGATAGT 
TCTAATTCCA TCAGGGAAAT AATTTACCCC AGTGGTCCCC AGGGCTCTTT GGATCCTCCT 
GTTCAAGAGC TTCCCCAGCC TATTTTGTTA GAGAAAAACT CTTTTGTTGC CACTGATCCT 
CAATCTTGTT CGAATACACA TGTTAAAGTG GTAGACAAGT CTCATGAAGA TTCTATTTTG 
GAAGAGGCAC GGGTTATAGA GGCTAAGCGC AAGAGGATTG CAGAATTATC TGTTGCATCT 
GTGCATTCAG AGAATCGCCG GAGATCTCAC TGGGATTTTG TCCTTGAAGA AATGGCATGG 
CTGGCAAATG ATGTTGCACA GGAGCGTCTC TGGAAGATGA CTGCTGCTGC TCAAATATGT 
CGACGAATTG CTTTTACTTC TCGATTGAGA GTTGAAGAGC AGAATCATCA TTTGAAGCTA 
AAAAACGTCG CTTACAGTCT TGCTAAGGCT GTCATGCAGT TCTGGCATTC AGCAAAGGTG 
TATCTAAGTA ACAATTGTCA CAGTGTTGGT TCAAAGAATG GGAAGCATGA GGTGGGGATG 
TTTGTTGGGA ATGAATTTTC TGTCAACAAG TTTGGAGATA TTGATAAGGA GCAGGTGGCA 
TGCAAAGAGT TAGAGAAACA AAATCCTGCC AAAAACATTG CGCATTCCAT TCATGGATAT 
GCTGTAAGGT TTTTGAAATA TAACAGTTCT CCATTTCCTT CCTTTCAAGC AGAAGCACCA 
GCAACTCCTG ATAGAATAGC TGATTTGGGC ATTGTGGACA CTTCATGGGA TGATCGCCTT 
ACTGAAGAAA GCCTGTTCTA CGCAGTTCCC TCAGGTGCCA TGGCAATGTA CAGATTGTCC 
ATTGAATCTC ATATTGCGCA GAGTGAGAAA ACTCGCAGTA GCATGCAGGA GGAAGTCGAT 
ACATCCATGT ATGACACTCC AGCAGATTTT GGATATCATG ATACTGCTGC ATACGATGAG 
GAGGAAGGAG AAACAAGTGC TTACTATATG CATGGAGTCT TTGAAGGTAG CAAGTCAGCA 
AAACATGACC AAAAGAAACG GAAAAGCTTA ACAAAGTCAC CCTCTGCAAG ATCTTATGAT 
CTGGGAACTG ATTCACCTTA TGGACACTGC ACCACTGGGC CACAACAAAA TGTATTGATG 
GGGAAAAGAC CTGCTAGTAA TCTTAATGCT GGCTCAATCC CAACAAAACG CATGCGTACT 
GCTTCTAGAC AGAGGTTTAC AAGTCCTTTT ACTGCTGGAA CTGCTGGGGT TTTACTCCAG 
GCTCCAGTGA AGACAGATGC ATCGAGTGGA GATACCAATT CTTTTCAGGA TGATCAGAGT 
ATTTTGCATG GTGGGTCACA AATTCAGAAA AGTGTGGAGG TTGAGTCCGC TGCGCATTTC 
GAAAGGCAGT TACCATATGA CTACGCAGAG ACATCAACAA AACCGAAGAA GAAGAAGAAG 
GCAAAGCACC TGGGTTCTGC TTATGAGCAG GGGTGGCAGT TGGATTCTAC TGGTCATAAT 
GAACAGAGGG ATAATTTCAA AAAGAGATCG GAGAGTCATC ATTTGGACTC TAATGGAACC 
AGTGGTTTAT ATGGGCAACA TACCACAAAG AAGCCAAAGA TATCTAAGCA ATTGCTAGAT 
AATACTTTTG ACAACATGGT TCAAATGACC GGGTCCATCC CATCTCCAGC TGCTTCCCAG 
ATGAGTAATA TGTCAAACAC AAATAGATTC ATCAAATTGA TTGGTGGCAG GGAACGAGGC 
AGAAAAAATA AATCAATGAA GATGTCTGTT GGGCAGCCAG GTTCTGGAAG TCCCTGGTCA 
CTTTTTGAAG ACCAGGCTCT TGTTGTTCTT GTGCATGACA TGGGTCCTAA TTGGGAGCTT 
ATAAGCGATG CTATCAACAG CACTGCTCAA TTCAAGTGTA TATTCCGCAA GCCTAAAGAA 
TGCAAAGATC GTCACAAAAT CTTAATGGAT AAGGGTGCTG GTGATGGGGC TGATAGTGCT 
GAGGATTCAG GATCTTCTCA ATCCTACCCA TCAACTTTGC CTGGCATTCC AAAGCAGGGC 
AGTGCCAGAC AGTTGTTTCA ACATTTGCAA GGGCCGATGC AAGAGGATAC CCTGAAGTCT 
CATTTTGAGA AAATCATCAT TATTGGGAAG AAACATCATT ACAAAAGGAG TCAGAATGAA 
AACCAGGATC CCAAGCAAAT AGCAGCAACT CACAATTCTC ATTTTATTGC TCTTTCCCAA 
GTTTGCCCAA ACAACTTAAA TGGAGGTGTC CTAACGCCCC TTGATCTCTG TGATTCAAGC 
ACTTCAAATC CAGATGTTCT TCCCAATGTG TATCAAGGTT CTCATGCTAG CAATTTGGTA 
ATGCCAAACC AAGGTGCTGT TGCATCAACG CTTCCCACTT CTGGTGCAAT CTCTTCTCTG 
CAAGGATCTT CTGGCGTGGT TCTTGGCAAT AACTCATCAT CACCATCTGG TCCCCTCAAT 
GCACCTCATA GAGATGGTAG ATACAATGTT CCAAGGACAT CTTTACCAGT TGACGAGCAT 
CAAAGAATGC AACCCTATCA AATGTTACCT AGTAGAAACT TGCAGCAGTC TAACATGTCG 
GTTTCTGGGG CTGTCTCTGG AGCTGATCGT GGTGTTCGCA TGTTATCGAG TGGAAATGGT 
ATGGGCATGA TGCCAGGGAT GAACAGAAGC ATGCCACTGC CACGGTCAGG ATTTCAAGGA 
ACAGCCTCTT CATCAATGCT GAATTCTGGA AGTATGCTTT CTAACAATGT GGTTGGGATG 
TCAAGCCCTG TGAATATGCA CACTGGTTCT GGTCAAGGGA ACTTGATGAG ACCTCGCGAG 
GCTTTGCATA TGTTGAGGCT TGGCCATAAT CATGAGCATC AAAGACAAAT GATGGTACCA 
GAGCTTCAGA TGCAGCCAAC ACAAGGGAAC AACCAAGGAA TTTCTGCTTT CAACGGGGTG 
CCTACTGCTT TTGCTAATCA GACAACTACT TCACCTGTGC AGACATATCC AGGCCATCCC 
CAGCAGCAGC ATCAAATGCC TGCACAACAG TCCAATATGC TTAGCAATCC TCATCATCCT 
AATCTCCGTG GCCCCAATCA GGCTACAGGT TCACAGCATC CAGCATATGC TATGCACCTG 
CCTAAAGGAA GGCAAATGCA GCAGCGGCTT CTCCAGCAGC AGCAGCAGCA GCAGCAGCAC 
TTTTCTGCAT CTAGTGCTTT GATGCCCCAT GTCCAACATC AATCCCAACT TCCCATATCA 
TCGTCTATGC AAAATAGTTC TCAAATCTCA CCACCATCTG CATCGCAGCC GGTGTCACTA 
CCTGCTATAA CTCCCCCTTC CCCAATGACT CCCATTTCAA TGCAGCAGCA ACAGCAGCAG 
AAACACAACT TGCCACATCA TGCGGTCAGT CGGAGCCCCC AAAGTGGTTC CAGTGGGTTG 
ACCAATCAGA TGGGAAAGCA GCGACAACGG CAACCACAGC AGTTTCAACA ATCTGGCAGA 
CATCACCCTC AACAACGACA ACACTCACAG TCTCCACAGC AGGCTAAACT TTTGAAGGGA 
ATGGGGAGAG GGAACATGGT GGTGCACCAG AACCTTCCCA ATGATCACTC TCCTTTGAAT 
GGCCTTTCTG TGCCTCCAGG AAACCATGGT GTAGAGAAAG GAGAGCAAAT CATGCACTTG 
ATGCAGGGTC AAGGCTTGTA TTCTGGGACT GGGTTGAGTC CAATTCATAC ATCTAAACCA 
TTGGGTCCTT CACAATCTCC AAACCATTCT CAGCCACAGC AAAAGCTATA TTCGGGCCCT 
ACAACCCCTT CATCAAAGCC ACTCCAGCAA ATGCCTTCTC ATTTGGAAAG TAGTACTCAA 
GGCCAGGTCC AACCAGTACC CTCTGGTCAA ACACTAACAG CTACTCACCA GAACACTCCA 
GTTATGGTTC CCAGTCATCA GCATCTTCAA CAACATCCAC AGCCACATCA GAAGCAGGTT 
AGTCAACCTC AACCAACTGT TCAGAGAATG CTTCAACAGA GTCGTCTGTT GAATTCTGAT 
CTGCCAACAA AGCCTCAAAC TGATCAGGGC CATGCTGATC AGCAAACTTC AAACAACATC 
TCCCAGACAG GAACAAGTAC ATCCACAGGG ATGCCTTTGG CCTGTAATGA TACATCTAAT 
GTGGCACCAG TTGTTTCTTC TGTTTCTGAG ATGCAGTGGA AATCATCAGA ACCATCCTTG 
CATGATTCTG GCATGGCAGA TACAGCTTCT AAGGTAGGTC CTATTGGTAG CCCCCCCCTT 
ACGAGTGCAG CTGGGAGTGA GCAAGTGGTG CCTGTCAGCC AAGGTTCAGT CCACAGGCAG 
TTATCAGGAG GCTTGCCCCT GCATTGCCAT AATGGTGGGA CACGGTGGCA GCACCAACAA 
CCACATCAAT CAACAGCATC GCCTTCCCCA TGCCAGCAAC TTTTGCAGTC ACAAGAGCCA 
CAACTACAGC AAGAACGACA ACAATCACCT CAACAGCTGC CACCACAGCA GCCGACTCAG 
AAGCAGACAC AGCATCTTCA ATCAGGACAA GGCAATTTAT ACATCACGCC AAGCAATTCT 
GGTCTGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7459484 ( XM_024595573.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024451341.1 Populus trichocarpa chromatin modification-related protein EAF1 B (LOC7459484), transcript variant X3, mRNA.

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CloneID	OPa424908
Clone ID Related Accession (Same CDS sequence)	XM_024595574.1 , XM_024595573.1 , XM_024595571.1 , XM_024595572.1
Accession Version	XM_024595573.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	chromatin modification-related protein EAF1 B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037286.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGGAT GTGGTTCAGG ATATGCTCTC CTAGTAAATG CTGAGGTTGA TTCCATGGGT 
GGGGTTGTTG ATGGTGGAGT TGGTATTGCT ACCAAAACCT CTCCGCGGCG AGCAGCAATT 
GAGAAGGCCC AGGTGGAACT TAGACAAGAG TATGATGTTC GTGAGGAACG GAGAAGGGAG 
CTAGAATTTC TTGAGAAAGG CGGCAATCCT TTGGATTTTA AATTTGTAAA TGCAACTTCG 
GTCAGTGTCC AGTCTACTTC ACTCACAGAT CATCACGTTG AACAGTTTGT TACTAGTGAA 
GCAAAAGGCA GTTTTCCACT GACTGCATCA CCTCATGGGG ATTCTGTAGA AAGTAGTGGC 
AGACCAGGGG CTACTCCAGT TTGTGAGCCC AATAGTGCTG ACAATTTTGA TGGTGAAAAT 
GAGTTACTTG AAGTTGAAAG GAAACGTAAA CATCCTAGTA GGAGGAATAA TGTCACTCAA 
TCAGAGCAGT CTTCACAGAT GGATGGAATT CACAATGCCA AGGAATCAGA AGATTCTGCT 
ATTTTTCGGC CATATGCTCG AAGGAATAGG TCCAGACCAA ATCGTGACGG CGCGCGATCA 
AGTTCAACTG ATATTGTTCA GAGTTCAGTT GGCCATGGAT CCTCTTTACC GGCTCGTGGG 
GATGCAAGGG ATGTGAAAGG GTTGGTAACT GAAACAGACG ATCATAAAGC CCAGATTATT 
ACTTCCATCT CTAATCCAAA GTCCACCACT TCAAACGGTG ACCTGTTTTT TCAGATTGAC 
ACTTCCAATA CACAGTCAAA CACGGAGCTT GATTGTGTGC AGGCTCTGAA AACAGTAGTC 
AACCTGCCTG ATGATAGGTT GGATGTTACA GAGTCCATTG TCTTGAGGGA TAACCAACAT 
GACCAGCCTT CAGAAGCTGA TGCTGAGAAA GCACCCAATG ATATTGCTTC CAGGGAATGC 
GATCATGGTG GAGGAAAAGA GCTGGTTATT TCAGCTGGTC CTGAATGTCC CCCTTGTGCT 
GAATCAGCCA AGACTGAGAA TGAAACTGGT CCTGCTCTGC TCAATGGCTT GGAAAAAGAT 
GGAAATGAAG GTCAAAATGG TAATGCAGCA ATGGGAACGG AACGATTCAA TTCAGAGTCC 
TCTTGCACCC AAAATAGCTT AAGCCTGGAT GCAAACAATG GCTGTGATCC TTGTGATAAT 
CGTAGAAATG ATGACACCAA TGAAATTCTT TTGAAAGAAT CATCAGAATT TGAAGGAACA 
CGAAGTTTAC CATCGGGTAA TATAGGAAAT GAAAAGAAAG AGACTAACTC GATCTCTGCA 
ATCAATGATG GTTCTGTGCA TGAAAACTAC TCTGGCAATG ATTCTACTGT CAAAAATGAG 
GAAGAAAGGA GAACAACTTT TCACTCTCAG GTGAAGTGTA CTAATCTTGA AGGAGTGGAA 
CAAAATGACC ATGTTGCCTC TGAGGCTGAT ACAAAGGCGG GCAACATGTT GGCTGATAGT 
TCTAATTCCA TCAGGGAAAT AATTTACCCC AGTGGTCCCC AGGGCTCTTT GGATCCTCCT 
GTTCAAGAGC TTCCCCAGCC TATTTTGTTA GAGAAAAACT CTTTTGTTGC CACTGATCCT 
CAATCTTGTT CGAATACACA TGTTAAAGTG GTAGACAAGT CTCATGAAGA TTCTATTTTG 
GAAGAGGCAC GGGTTATAGA GGCTAAGCGC AAGAGGATTG CAGAATTATC TGTTGCATCT 
GTGCATTCAG AGAATCGCCG GAGATCTCAC TGGGATTTTG TCCTTGAAGA AATGGCATGG 
CTGGCAAATG ATGTTGCACA GGAGCGTCTC TGGAAGATGA CTGCTGCTGC TCAAATATGT 
CGACGAATTG CTTTTACTTC TCGATTGAGA GTTGAAGAGC AGAATCATCA TTTGAAGCTA 
AAAAACGTCG CTTACAGTCT TGCTAAGGCT GTCATGCAGT TCTGGCATTC AGCAAAGGTG 
TATCTAAGTA ACAATTGTCA CAGTGTTGGT TCAAAGAATG GGAAGCATGA GGTGGGGATG 
TTTGTTGGGA ATGAATTTTC TGTCAACAAG TTTGGAGATA TTGATAAGGA GCAGGTGGCA 
TGCAAAGAGT TAGAGAAACA AAATCCTGCC AAAAACATTG CGCATTCCAT TCATGGATAT 
GCTGTAAGGT TTTTGAAATA TAACAGTTCT CCATTTCCTT CCTTTCAAGC AGAAGCACCA 
GCAACTCCTG ATAGAATAGC TGATTTGGGC ATTGTGGACA CTTCATGGGA TGATCGCCTT 
ACTGAAGAAA GCCTGTTCTA CGCAGTTCCC TCAGGTGCCA TGGCAATGTA CAGATTGTCC 
ATTGAATCTC ATATTGCGCA GAGTGAGAAA ACTCGCAGTA GCATGCAGGA GGAAGTCGAT 
ACATCCATGT ATGACACTCC AGCAGATTTT GGATATCATG ATACTGCTGC ATACGATGAG 
GAGGAAGGAG AAACAAGTGC TTACTATATG CATGGAGTCT TTGAAGGTAG CAAGTCAGCA 
AAACATGACC AAAAGAAACG GAAAAGCTTA ACAAAGTCAC CCTCTGCAAG ATCTTATGAT 
CTGGGAACTG ATTCACCTTA TGGACACTGC ACCACTGGGC CACAACAAAA TGTATTGATG 
GGGAAAAGAC CTGCTAGTAA TCTTAATGCT GGCTCAATCC CAACAAAACG CATGCGTACT 
GCTTCTAGAC AGAGGTTTAC AAGTCCTTTT ACTGCTGGAA CTGCTGGGGT TTTACTCCAG 
GCTCCAGTGA AGACAGATGC ATCGAGTGGA GATACCAATT CTTTTCAGGA TGATCAGAGT 
ATTTTGCATG GTGGGTCACA AATTCAGAAA AGTGTGGAGG TTGAGTCCGC TGCGCATTTC 
GAAAGGCAGT TACCATATGA CTACGCAGAG ACATCAACAA AACCGAAGAA GAAGAAGAAG 
GCAAAGCACC TGGGTTCTGC TTATGAGCAG GGGTGGCAGT TGGATTCTAC TGGTCATAAT 
GAACAGAGGG ATAATTTCAA AAAGAGATCG GAGAGTCATC ATTTGGACTC TAATGGAACC 
AGTGGTTTAT ATGGGCAACA TACCACAAAG AAGCCAAAGA TATCTAAGCA ATTGCTAGAT 
AATACTTTTG ACAACATGGT TCAAATGACC GGGTCCATCC CATCTCCAGC TGCTTCCCAG 
ATGAGTAATA TGTCAAACAC AAATAGATTC ATCAAATTGA TTGGTGGCAG GGAACGAGGC 
AGAAAAAATA AATCAATGAA GATGTCTGTT GGGCAGCCAG GTTCTGGAAG TCCCTGGTCA 
CTTTTTGAAG ACCAGGCTCT TGTTGTTCTT GTGCATGACA TGGGTCCTAA TTGGGAGCTT 
ATAAGCGATG CTATCAACAG CACTGCTCAA TTCAAGTGTA TATTCCGCAA GCCTAAAGAA 
TGCAAAGATC GTCACAAAAT CTTAATGGAT AAGGGTGCTG GTGATGGGGC TGATAGTGCT 
GAGGATTCAG GATCTTCTCA ATCCTACCCA TCAACTTTGC CTGGCATTCC AAAGCAGGGC 
AGTGCCAGAC AGTTGTTTCA ACATTTGCAA GGGCCGATGC AAGAGGATAC CCTGAAGTCT 
CATTTTGAGA AAATCATCAT TATTGGGAAG AAACATCATT ACAAAAGGAG TCAGAATGAA 
AACCAGGATC CCAAGCAAAT AGCAGCAACT CACAATTCTC ATTTTATTGC TCTTTCCCAA 
GTTTGCCCAA ACAACTTAAA TGGAGGTGTC CTAACGCCCC TTGATCTCTG TGATTCAAGC 
ACTTCAAATC CAGATGTTCT TCCCAATGTG TATCAAGGTT CTCATGCTAG CAATTTGGTA 
ATGCCAAACC AAGGTGCTGT TGCATCAACG CTTCCCACTT CTGGTGCAAT CTCTTCTCTG 
CAAGGATCTT CTGGCGTGGT TCTTGGCAAT AACTCATCAT CACCATCTGG TCCCCTCAAT 
GCACCTCATA GAGATGGTAG ATACAATGTT CCAAGGACAT CTTTACCAGT TGACGAGCAT 
CAAAGAATGC AACCCTATCA AATGTTACCT AGTAGAAACT TGCAGCAGTC TAACATGTCG 
GTTTCTGGGG CTGTCTCTGG AGCTGATCGT GGTGTTCGCA TGTTATCGAG TGGAAATGGT 
ATGGGCATGA TGCCAGGGAT GAACAGAAGC ATGCCACTGC CACGGTCAGG ATTTCAAGGA 
ACAGCCTCTT CATCAATGCT GAATTCTGGA AGTATGCTTT CTAACAATGT GGTTGGGATG 
TCAAGCCCTG TGAATATGCA CACTGGTTCT GGTCAAGGGA ACTTGATGAG ACCTCGCGAG 
GCTTTGCATA TGTTGAGGCT TGGCCATAAT CATGAGCATC AAAGACAAAT GATGGTACCA 
GAGCTTCAGA TGCAGCCAAC ACAAGGGAAC AACCAAGGAA TTTCTGCTTT CAACGGGGTG 
CCTACTGCTT TTGCTAATCA GACAACTACT TCACCTGTGC AGACATATCC AGGCCATCCC 
CAGCAGCAGC ATCAAATGCC TGCACAACAG TCCAATATGC TTAGCAATCC TCATCATCCT 
AATCTCCGTG GCCCCAATCA GGCTACAGGT TCACAGCATC CAGCATATGC TATGCACCTG 
CCTAAAGGAA GGCAAATGCA GCAGCGGCTT CTCCAGCAGC AGCAGCAGCA GCAGCAGCAC 
TTTTCTGCAT CTAGTGCTTT GATGCCCCAT GTCCAACATC AATCCCAACT TCCCATATCA 
TCGTCTATGC AAAATAGTTC TCAAATCTCA CCACCATCTG CATCGCAGCC GGTGTCACTA 
CCTGCTATAA CTCCCCCTTC CCCAATGACT CCCATTTCAA TGCAGCAGCA ACAGCAGCAG 
AAACACAACT TGCCACATCA TGCGGTCAGT CGGAGCCCCC AAAGTGGTTC CAGTGGGTTG 
ACCAATCAGA TGGGAAAGCA GCGACAACGG CAACCACAGC AGTTTCAACA ATCTGGCAGA 
CATCACCCTC AACAACGACA ACACTCACAG TCTCCACAGC AGGCTAAACT TTTGAAGGGA 
ATGGGGAGAG GGAACATGGT GGTGCACCAG AACCTTCCCA ATGATCACTC TCCTTTGAAT 
GGCCTTTCTG TGCCTCCAGG AAACCATGGT GTAGAGAAAG GAGAGCAAAT CATGCACTTG 
ATGCAGGGTC AAGGCTTGTA TTCTGGGACT GGGTTGAGTC CAATTCATAC ATCTAAACCA 
TTGGGTCCTT CACAATCTCC AAACCATTCT CAGCCACAGC AAAAGCTATA TTCGGGCCCT 
ACAACCCCTT CATCAAAGCC ACTCCAGCAA ATGCCTTCTC ATTTGGAAAG TAGTACTCAA 
GGCCAGGTCC AACCAGTACC CTCTGGTCAA ACACTAACAG CTACTCACCA GAACACTCCA 
GTTATGGTTC CCAGTCATCA GCATCTTCAA CAACATCCAC AGCCACATCA GAAGCAGGTT 
AGTCAACCTC AACCAACTGT TCAGAGAATG CTTCAACAGA GTCGTCTGTT GAATTCTGAT 
CTGCCAACAA AGCCTCAAAC TGATCAGGGC CATGCTGATC AGCAAACTTC AAACAACATC 
TCCCAGACAG GAACAAGTAC ATCCACAGGG ATGCCTTTGG CCTGTAATGA TACATCTAAT 
GTGGCACCAG TTGTTTCTTC TGTTTCTGAG ATGCAGTGGA AATCATCAGA ACCATCCTTG 
CATGATTCTG GCATGGCAGA TACAGCTTCT AAGGTAGGTC CTATTGGTAG CCCCCCCCTT 
ACGAGTGCAG CTGGGAGTGA GCAAGTGGTG CCTGTCAGCC AAGGTTCAGT CCACAGGCAG 
TTATCAGGAG GCTTGCCCCT GCATTGCCAT AATGGTGGGA CACGGTGGCA GCACCAACAA 
CCACATCAAT CAACAGCATC GCCTTCCCCA TGCCAGCAAC TTTTGCAGTC ACAAGAGCCA 
CAACTACAGC AAGAACGACA ACAATCACCT CAACAGCTGC CACCACAGCA GCCGACTCAG 
AAGCAGACAC AGCATCTTCA ATCAGGACAA GGCAATTTAT ACATCACGCC AAGCAATTCT 
GGTCTGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024451341.1
CDS	401..6472
Translation	MHGCGSGYALLVNAEVDSMGGVVDGGVGIATKTSPRRAAIEKAQVELRQEYDVREERRRELEFLEKGGNPLDFKFVNATSVSVQSTSLTDHHVEQFVTSEAKGSFPLTASPHGDSVESSGRPGATPVCEPNSADNFDGENELLEVERKRKHPSRRNNVTQSEQSSQMDGIHNAKESEDSAIFRPYARRNRSRPNRDGARSSSTDIVQSSVGHGSSLPARGDARDVKGLVTETDDHKAQIITSISNPKSTTSNGDLFFQIDTSNTQSNTELDCVQALKTVVNLPDDRLDVTESIVLRDNQHDQPSEADAEKAPNDIASRECDHGGGKELVISAGPECPPCAESAKTENETGPALLNGLEKDGNEGQNGNAAMGTERFNSESSCTQNSLSLDANNGCDPCDNRRNDDTNEILLKESSEFEGTRSLPSGNIGNEKKETNSISAINDGSVHENYSGNDSTVKNEEERRTTFHSQVKCTNLEGVEQNDHVASEADTKAGNMLADSSNSIREIIYPSGPQGSLDPPVQELPQPILLEKNSFVATDPQSCSNTHVKVVDKSHEDSILEEARVIEAKRKRIAELSVASVHSENRRRSHWDFVLEEMAWLANDVAQERLWKMTAAAQICRRIAFTSRLRVEEQNHHLKLKNVAYSLAKAVMQFWHSAKVYLSNNCHSVGSKNGKHEVGMFVGNEFSVNKFGDIDKEQVACKELEKQNPAKNIAHSIHGYAVRFLKYNSSPFPSFQAEAPATPDRIADLGIVDTSWDDRLTEESLFYAVPSGAMAMYRLSIESHIAQSEKTRSSMQEEVDTSMYDTPADFGYHDTAAYDEEEGETSAYYMHGVFEGSKSAKHDQKKRKSLTKSPSARSYDLGTDSPYGHCTTGPQQNVLMGKRPASNLNAGSIPTKRMRTASRQRFTSPFTAGTAGVLLQAPVKTDASSGDTNSFQDDQSILHGGSQIQKSVEVESAAHFERQLPYDYAETSTKPKKKKKAKHLGSAYEQGWQLDSTGHNEQRDNFKKRSESHHLDSNGTSGLYGQHTTKKPKISKQLLDNTFDNMVQMTGSIPSPAASQMSNMSNTNRFIKLIGGRERGRKNKSMKMSVGQPGSGSPWSLFEDQALVVLVHDMGPNWELISDAINSTAQFKCIFRKPKECKDRHKILMDKGAGDGADSAEDSGSSQSYPSTLPGIPKQGSARQLFQHLQGPMQEDTLKSHFEKIIIIGKKHHYKRSQNENQDPKQIAATHNSHFIALSQVCPNNLNGGVLTPLDLCDSSTSNPDVLPNVYQGSHASNLVMPNQGAVASTLPTSGAISSLQGSSGVVLGNNSSSPSGPLNAPHRDGRYNVPRTSLPVDEHQRMQPYQMLPSRNLQQSNMSVSGAVSGADRGVRMLSSGNGMGMMPGMNRSMPLPRSGFQGTASSSMLNSGSMLSNNVVGMSSPVNMHTGSGQGNLMRPREALHMLRLGHNHEHQRQMMVPELQMQPTQGNNQGISAFNGVPTAFANQTTTSPVQTYPGHPQQQHQMPAQQSNMLSNPHHPNLRGPNQATGSQHPAYAMHLPKGRQMQQRLLQQQQQQQQHFSASSALMPHVQHQSQLPISSSMQNSSQISPPSASQPVSLPAITPPSPMTPISMQQQQQQKHNLPHHAVSRSPQSGSSGLTNQMGKQRQRQPQQFQQSGRHHPQQRQHSQSPQQAKLLKGMGRGNMVVHQNLPNDHSPLNGLSVPPGNHGVEKGEQIMHLMQGQGLYSGTGLSPIHTSKPLGPSQSPNHSQPQQKLYSGPTTPSSKPLQQMPSHLESSTQGQVQPVPSGQTLTATHQNTPVMVPSHQHLQQHPQPHQKQVSQPQPTVQRMLQQSRLLNSDLPTKPQTDQGHADQQTSNNISQTGTSTSTGMPLACNDTSNVAPVVSSVSEMQWKSSEPSLHDSGMADTASKVGPIGSPPLTSAAGSEQVVPVSQGSVHRQLSGGLPLHCHNGGTRWQHQQPHQSTASPSPCQQLLQSQEPQLQQERQQSPQQLPPQQPTQKQTQHLQSGQGNLYITPSNSGLE

Target ORF information:

RefSeq Version	XM_024595573.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa chromatin modification-related protein EAF1 B (LOC7459484), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024595573.1

ORF Insert Sequence:

ATGCATGGAT GTGGTTCAGG ATATGCTCTC CTAGTAAATG CTGAGGTTGA TTCCATGGGT 
GGGGTTGTTG ATGGTGGAGT TGGTATTGCT ACCAAAACCT CTCCGCGGCG AGCAGCAATT 
GAGAAGGCCC AGGTGGAACT TAGACAAGAG TATGATGTTC GTGAGGAACG GAGAAGGGAG 
CTAGAATTTC TTGAGAAAGG CGGCAATCCT TTGGATTTTA AATTTGTAAA TGCAACTTCG 
GTCAGTGTCC AGTCTACTTC ACTCACAGAT CATCACGTTG AACAGTTTGT TACTAGTGAA 
GCAAAAGGCA GTTTTCCACT GACTGCATCA CCTCATGGGG ATTCTGTAGA AAGTAGTGGC 
AGACCAGGGG CTACTCCAGT TTGTGAGCCC AATAGTGCTG ACAATTTTGA TGGTGAAAAT 
GAGTTACTTG AAGTTGAAAG GAAACGTAAA CATCCTAGTA GGAGGAATAA TGTCACTCAA 
TCAGAGCAGT CTTCACAGAT GGATGGAATT CACAATGCCA AGGAATCAGA AGATTCTGCT 
ATTTTTCGGC CATATGCTCG AAGGAATAGG TCCAGACCAA ATCGTGACGG CGCGCGATCA 
AGTTCAACTG ATATTGTTCA GAGTTCAGTT GGCCATGGAT CCTCTTTACC GGCTCGTGGG 
GATGCAAGGG ATGTGAAAGG GTTGGTAACT GAAACAGACG ATCATAAAGC CCAGATTATT 
ACTTCCATCT CTAATCCAAA GTCCACCACT TCAAACGGTG ACCTGTTTTT TCAGATTGAC 
ACTTCCAATA CACAGTCAAA CACGGAGCTT GATTGTGTGC AGGCTCTGAA AACAGTAGTC 
AACCTGCCTG ATGATAGGTT GGATGTTACA GAGTCCATTG TCTTGAGGGA TAACCAACAT 
GACCAGCCTT CAGAAGCTGA TGCTGAGAAA GCACCCAATG ATATTGCTTC CAGGGAATGC 
GATCATGGTG GAGGAAAAGA GCTGGTTATT TCAGCTGGTC CTGAATGTCC CCCTTGTGCT 
GAATCAGCCA AGACTGAGAA TGAAACTGGT CCTGCTCTGC TCAATGGCTT GGAAAAAGAT 
GGAAATGAAG GTCAAAATGG TAATGCAGCA ATGGGAACGG AACGATTCAA TTCAGAGTCC 
TCTTGCACCC AAAATAGCTT AAGCCTGGAT GCAAACAATG GCTGTGATCC TTGTGATAAT 
CGTAGAAATG ATGACACCAA TGAAATTCTT TTGAAAGAAT CATCAGAATT TGAAGGAACA 
CGAAGTTTAC CATCGGGTAA TATAGGAAAT GAAAAGAAAG AGACTAACTC GATCTCTGCA 
ATCAATGATG GTTCTGTGCA TGAAAACTAC TCTGGCAATG ATTCTACTGT CAAAAATGAG 
GAAGAAAGGA GAACAACTTT TCACTCTCAG GTGAAGTGTA CTAATCTTGA AGGAGTGGAA 
CAAAATGACC ATGTTGCCTC TGAGGCTGAT ACAAAGGCGG GCAACATGTT GGCTGATAGT 
TCTAATTCCA TCAGGGAAAT AATTTACCCC AGTGGTCCCC AGGGCTCTTT GGATCCTCCT 
GTTCAAGAGC TTCCCCAGCC TATTTTGTTA GAGAAAAACT CTTTTGTTGC CACTGATCCT 
CAATCTTGTT CGAATACACA TGTTAAAGTG GTAGACAAGT CTCATGAAGA TTCTATTTTG 
GAAGAGGCAC GGGTTATAGA GGCTAAGCGC AAGAGGATTG CAGAATTATC TGTTGCATCT 
GTGCATTCAG AGAATCGCCG GAGATCTCAC TGGGATTTTG TCCTTGAAGA AATGGCATGG 
CTGGCAAATG ATGTTGCACA GGAGCGTCTC TGGAAGATGA CTGCTGCTGC TCAAATATGT 
CGACGAATTG CTTTTACTTC TCGATTGAGA GTTGAAGAGC AGAATCATCA TTTGAAGCTA 
AAAAACGTCG CTTACAGTCT TGCTAAGGCT GTCATGCAGT TCTGGCATTC AGCAAAGGTG 
TATCTAAGTA ACAATTGTCA CAGTGTTGGT TCAAAGAATG GGAAGCATGA GGTGGGGATG 
TTTGTTGGGA ATGAATTTTC TGTCAACAAG TTTGGAGATA TTGATAAGGA GCAGGTGGCA 
TGCAAAGAGT TAGAGAAACA AAATCCTGCC AAAAACATTG CGCATTCCAT TCATGGATAT 
GCTGTAAGGT TTTTGAAATA TAACAGTTCT CCATTTCCTT CCTTTCAAGC AGAAGCACCA 
GCAACTCCTG ATAGAATAGC TGATTTGGGC ATTGTGGACA CTTCATGGGA TGATCGCCTT 
ACTGAAGAAA GCCTGTTCTA CGCAGTTCCC TCAGGTGCCA TGGCAATGTA CAGATTGTCC 
ATTGAATCTC ATATTGCGCA GAGTGAGAAA ACTCGCAGTA GCATGCAGGA GGAAGTCGAT 
ACATCCATGT ATGACACTCC AGCAGATTTT GGATATCATG ATACTGCTGC ATACGATGAG 
GAGGAAGGAG AAACAAGTGC TTACTATATG CATGGAGTCT TTGAAGGTAG CAAGTCAGCA 
AAACATGACC AAAAGAAACG GAAAAGCTTA ACAAAGTCAC CCTCTGCAAG ATCTTATGAT 
CTGGGAACTG ATTCACCTTA TGGACACTGC ACCACTGGGC CACAACAAAA TGTATTGATG 
GGGAAAAGAC CTGCTAGTAA TCTTAATGCT GGCTCAATCC CAACAAAACG CATGCGTACT 
GCTTCTAGAC AGAGGTTTAC AAGTCCTTTT ACTGCTGGAA CTGCTGGGGT TTTACTCCAG 
GCTCCAGTGA AGACAGATGC ATCGAGTGGA GATACCAATT CTTTTCAGGA TGATCAGAGT 
ATTTTGCATG GTGGGTCACA AATTCAGAAA AGTGTGGAGG TTGAGTCCGC TGCGCATTTC 
GAAAGGCAGT TACCATATGA CTACGCAGAG ACATCAACAA AACCGAAGAA GAAGAAGAAG 
GCAAAGCACC TGGGTTCTGC TTATGAGCAG GGGTGGCAGT TGGATTCTAC TGGTCATAAT 
GAACAGAGGG ATAATTTCAA AAAGAGATCG GAGAGTCATC ATTTGGACTC TAATGGAACC 
AGTGGTTTAT ATGGGCAACA TACCACAAAG AAGCCAAAGA TATCTAAGCA ATTGCTAGAT 
AATACTTTTG ACAACATGGT TCAAATGACC GGGTCCATCC CATCTCCAGC TGCTTCCCAG 
ATGAGTAATA TGTCAAACAC AAATAGATTC ATCAAATTGA TTGGTGGCAG GGAACGAGGC 
AGAAAAAATA AATCAATGAA GATGTCTGTT GGGCAGCCAG GTTCTGGAAG TCCCTGGTCA 
CTTTTTGAAG ACCAGGCTCT TGTTGTTCTT GTGCATGACA TGGGTCCTAA TTGGGAGCTT 
ATAAGCGATG CTATCAACAG CACTGCTCAA TTCAAGTGTA TATTCCGCAA GCCTAAAGAA 
TGCAAAGATC GTCACAAAAT CTTAATGGAT AAGGGTGCTG GTGATGGGGC TGATAGTGCT 
GAGGATTCAG GATCTTCTCA ATCCTACCCA TCAACTTTGC CTGGCATTCC AAAGCAGGGC 
AGTGCCAGAC AGTTGTTTCA ACATTTGCAA GGGCCGATGC AAGAGGATAC CCTGAAGTCT 
CATTTTGAGA AAATCATCAT TATTGGGAAG AAACATCATT ACAAAAGGAG TCAGAATGAA 
AACCAGGATC CCAAGCAAAT AGCAGCAACT CACAATTCTC ATTTTATTGC TCTTTCCCAA 
GTTTGCCCAA ACAACTTAAA TGGAGGTGTC CTAACGCCCC TTGATCTCTG TGATTCAAGC 
ACTTCAAATC CAGATGTTCT TCCCAATGTG TATCAAGGTT CTCATGCTAG CAATTTGGTA 
ATGCCAAACC AAGGTGCTGT TGCATCAACG CTTCCCACTT CTGGTGCAAT CTCTTCTCTG 
CAAGGATCTT CTGGCGTGGT TCTTGGCAAT AACTCATCAT CACCATCTGG TCCCCTCAAT 
GCACCTCATA GAGATGGTAG ATACAATGTT CCAAGGACAT CTTTACCAGT TGACGAGCAT 
CAAAGAATGC AACCCTATCA AATGTTACCT AGTAGAAACT TGCAGCAGTC TAACATGTCG 
GTTTCTGGGG CTGTCTCTGG AGCTGATCGT GGTGTTCGCA TGTTATCGAG TGGAAATGGT 
ATGGGCATGA TGCCAGGGAT GAACAGAAGC ATGCCACTGC CACGGTCAGG ATTTCAAGGA 
ACAGCCTCTT CATCAATGCT GAATTCTGGA AGTATGCTTT CTAACAATGT GGTTGGGATG 
TCAAGCCCTG TGAATATGCA CACTGGTTCT GGTCAAGGGA ACTTGATGAG ACCTCGCGAG 
GCTTTGCATA TGTTGAGGCT TGGCCATAAT CATGAGCATC AAAGACAAAT GATGGTACCA 
GAGCTTCAGA TGCAGCCAAC ACAAGGGAAC AACCAAGGAA TTTCTGCTTT CAACGGGGTG 
CCTACTGCTT TTGCTAATCA GACAACTACT TCACCTGTGC AGACATATCC AGGCCATCCC 
CAGCAGCAGC ATCAAATGCC TGCACAACAG TCCAATATGC TTAGCAATCC TCATCATCCT 
AATCTCCGTG GCCCCAATCA GGCTACAGGT TCACAGCATC CAGCATATGC TATGCACCTG 
CCTAAAGGAA GGCAAATGCA GCAGCGGCTT CTCCAGCAGC AGCAGCAGCA GCAGCAGCAC 
TTTTCTGCAT CTAGTGCTTT GATGCCCCAT GTCCAACATC AATCCCAACT TCCCATATCA 
TCGTCTATGC AAAATAGTTC TCAAATCTCA CCACCATCTG CATCGCAGCC GGTGTCACTA 
CCTGCTATAA CTCCCCCTTC CCCAATGACT CCCATTTCAA TGCAGCAGCA ACAGCAGCAG 
AAACACAACT TGCCACATCA TGCGGTCAGT CGGAGCCCCC AAAGTGGTTC CAGTGGGTTG 
ACCAATCAGA TGGGAAAGCA GCGACAACGG CAACCACAGC AGTTTCAACA ATCTGGCAGA 
CATCACCCTC AACAACGACA ACACTCACAG TCTCCACAGC AGGCTAAACT TTTGAAGGGA 
ATGGGGAGAG GGAACATGGT GGTGCACCAG AACCTTCCCA ATGATCACTC TCCTTTGAAT 
GGCCTTTCTG TGCCTCCAGG AAACCATGGT GTAGAGAAAG GAGAGCAAAT CATGCACTTG 
ATGCAGGGTC AAGGCTTGTA TTCTGGGACT GGGTTGAGTC CAATTCATAC ATCTAAACCA 
TTGGGTCCTT CACAATCTCC AAACCATTCT CAGCCACAGC AAAAGCTATA TTCGGGCCCT 
ACAACCCCTT CATCAAAGCC ACTCCAGCAA ATGCCTTCTC ATTTGGAAAG TAGTACTCAA 
GGCCAGGTCC AACCAGTACC CTCTGGTCAA ACACTAACAG CTACTCACCA GAACACTCCA 
GTTATGGTTC CCAGTCATCA GCATCTTCAA CAACATCCAC AGCCACATCA GAAGCAGGTT 
AGTCAACCTC AACCAACTGT TCAGAGAATG CTTCAACAGA GTCGTCTGTT GAATTCTGAT 
CTGCCAACAA AGCCTCAAAC TGATCAGGGC CATGCTGATC AGCAAACTTC AAACAACATC 
TCCCAGACAG GAACAAGTAC ATCCACAGGG ATGCCTTTGG CCTGTAATGA TACATCTAAT 
GTGGCACCAG TTGTTTCTTC TGTTTCTGAG ATGCAGTGGA AATCATCAGA ACCATCCTTG 
CATGATTCTG GCATGGCAGA TACAGCTTCT AAGGTAGGTC CTATTGGTAG CCCCCCCCTT 
ACGAGTGCAG CTGGGAGTGA GCAAGTGGTG CCTGTCAGCC AAGGTTCAGT CCACAGGCAG 
TTATCAGGAG GCTTGCCCCT GCATTGCCAT AATGGTGGGA CACGGTGGCA GCACCAACAA 
CCACATCAAT CAACAGCATC GCCTTCCCCA TGCCAGCAAC TTTTGCAGTC ACAAGAGCCA 
CAACTACAGC AAGAACGACA ACAATCACCT CAACAGCTGC CACCACAGCA GCCGACTCAG 
AAGCAGACAC AGCATCTTCA ATCAGGACAA GGCAATTTAT ACATCACGCC AAGCAATTCT 
GGTCTGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7459484 ( XM_024595571.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024451339.1 Populus trichocarpa chromatin modification-related protein EAF1 B (LOC7459484), transcript variant X1, mRNA.

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CloneID	OPa424908
Clone ID Related Accession (Same CDS sequence)	XM_024595574.1 , XM_024595573.1 , XM_024595571.1 , XM_024595572.1
Accession Version	XM_024595571.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	chromatin modification-related protein EAF1 B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037286.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGGAT GTGGTTCAGG ATATGCTCTC CTAGTAAATG CTGAGGTTGA TTCCATGGGT 
GGGGTTGTTG ATGGTGGAGT TGGTATTGCT ACCAAAACCT CTCCGCGGCG AGCAGCAATT 
GAGAAGGCCC AGGTGGAACT TAGACAAGAG TATGATGTTC GTGAGGAACG GAGAAGGGAG 
CTAGAATTTC TTGAGAAAGG CGGCAATCCT TTGGATTTTA AATTTGTAAA TGCAACTTCG 
GTCAGTGTCC AGTCTACTTC ACTCACAGAT CATCACGTTG AACAGTTTGT TACTAGTGAA 
GCAAAAGGCA GTTTTCCACT GACTGCATCA CCTCATGGGG ATTCTGTAGA AAGTAGTGGC 
AGACCAGGGG CTACTCCAGT TTGTGAGCCC AATAGTGCTG ACAATTTTGA TGGTGAAAAT 
GAGTTACTTG AAGTTGAAAG GAAACGTAAA CATCCTAGTA GGAGGAATAA TGTCACTCAA 
TCAGAGCAGT CTTCACAGAT GGATGGAATT CACAATGCCA AGGAATCAGA AGATTCTGCT 
ATTTTTCGGC CATATGCTCG AAGGAATAGG TCCAGACCAA ATCGTGACGG CGCGCGATCA 
AGTTCAACTG ATATTGTTCA GAGTTCAGTT GGCCATGGAT CCTCTTTACC GGCTCGTGGG 
GATGCAAGGG ATGTGAAAGG GTTGGTAACT GAAACAGACG ATCATAAAGC CCAGATTATT 
ACTTCCATCT CTAATCCAAA GTCCACCACT TCAAACGGTG ACCTGTTTTT TCAGATTGAC 
ACTTCCAATA CACAGTCAAA CACGGAGCTT GATTGTGTGC AGGCTCTGAA AACAGTAGTC 
AACCTGCCTG ATGATAGGTT GGATGTTACA GAGTCCATTG TCTTGAGGGA TAACCAACAT 
GACCAGCCTT CAGAAGCTGA TGCTGAGAAA GCACCCAATG ATATTGCTTC CAGGGAATGC 
GATCATGGTG GAGGAAAAGA GCTGGTTATT TCAGCTGGTC CTGAATGTCC CCCTTGTGCT 
GAATCAGCCA AGACTGAGAA TGAAACTGGT CCTGCTCTGC TCAATGGCTT GGAAAAAGAT 
GGAAATGAAG GTCAAAATGG TAATGCAGCA ATGGGAACGG AACGATTCAA TTCAGAGTCC 
TCTTGCACCC AAAATAGCTT AAGCCTGGAT GCAAACAATG GCTGTGATCC TTGTGATAAT 
CGTAGAAATG ATGACACCAA TGAAATTCTT TTGAAAGAAT CATCAGAATT TGAAGGAACA 
CGAAGTTTAC CATCGGGTAA TATAGGAAAT GAAAAGAAAG AGACTAACTC GATCTCTGCA 
ATCAATGATG GTTCTGTGCA TGAAAACTAC TCTGGCAATG ATTCTACTGT CAAAAATGAG 
GAAGAAAGGA GAACAACTTT TCACTCTCAG GTGAAGTGTA CTAATCTTGA AGGAGTGGAA 
CAAAATGACC ATGTTGCCTC TGAGGCTGAT ACAAAGGCGG GCAACATGTT GGCTGATAGT 
TCTAATTCCA TCAGGGAAAT AATTTACCCC AGTGGTCCCC AGGGCTCTTT GGATCCTCCT 
GTTCAAGAGC TTCCCCAGCC TATTTTGTTA GAGAAAAACT CTTTTGTTGC CACTGATCCT 
CAATCTTGTT CGAATACACA TGTTAAAGTG GTAGACAAGT CTCATGAAGA TTCTATTTTG 
GAAGAGGCAC GGGTTATAGA GGCTAAGCGC AAGAGGATTG CAGAATTATC TGTTGCATCT 
GTGCATTCAG AGAATCGCCG GAGATCTCAC TGGGATTTTG TCCTTGAAGA AATGGCATGG 
CTGGCAAATG ATGTTGCACA GGAGCGTCTC TGGAAGATGA CTGCTGCTGC TCAAATATGT 
CGACGAATTG CTTTTACTTC TCGATTGAGA GTTGAAGAGC AGAATCATCA TTTGAAGCTA 
AAAAACGTCG CTTACAGTCT TGCTAAGGCT GTCATGCAGT TCTGGCATTC AGCAAAGGTG 
TATCTAAGTA ACAATTGTCA CAGTGTTGGT TCAAAGAATG GGAAGCATGA GGTGGGGATG 
TTTGTTGGGA ATGAATTTTC TGTCAACAAG TTTGGAGATA TTGATAAGGA GCAGGTGGCA 
TGCAAAGAGT TAGAGAAACA AAATCCTGCC AAAAACATTG CGCATTCCAT TCATGGATAT 
GCTGTAAGGT TTTTGAAATA TAACAGTTCT CCATTTCCTT CCTTTCAAGC AGAAGCACCA 
GCAACTCCTG ATAGAATAGC TGATTTGGGC ATTGTGGACA CTTCATGGGA TGATCGCCTT 
ACTGAAGAAA GCCTGTTCTA CGCAGTTCCC TCAGGTGCCA TGGCAATGTA CAGATTGTCC 
ATTGAATCTC ATATTGCGCA GAGTGAGAAA ACTCGCAGTA GCATGCAGGA GGAAGTCGAT 
ACATCCATGT ATGACACTCC AGCAGATTTT GGATATCATG ATACTGCTGC ATACGATGAG 
GAGGAAGGAG AAACAAGTGC TTACTATATG CATGGAGTCT TTGAAGGTAG CAAGTCAGCA 
AAACATGACC AAAAGAAACG GAAAAGCTTA ACAAAGTCAC CCTCTGCAAG ATCTTATGAT 
CTGGGAACTG ATTCACCTTA TGGACACTGC ACCACTGGGC CACAACAAAA TGTATTGATG 
GGGAAAAGAC CTGCTAGTAA TCTTAATGCT GGCTCAATCC CAACAAAACG CATGCGTACT 
GCTTCTAGAC AGAGGTTTAC AAGTCCTTTT ACTGCTGGAA CTGCTGGGGT TTTACTCCAG 
GCTCCAGTGA AGACAGATGC ATCGAGTGGA GATACCAATT CTTTTCAGGA TGATCAGAGT 
ATTTTGCATG GTGGGTCACA AATTCAGAAA AGTGTGGAGG TTGAGTCCGC TGCGCATTTC 
GAAAGGCAGT TACCATATGA CTACGCAGAG ACATCAACAA AACCGAAGAA GAAGAAGAAG 
GCAAAGCACC TGGGTTCTGC TTATGAGCAG GGGTGGCAGT TGGATTCTAC TGGTCATAAT 
GAACAGAGGG ATAATTTCAA AAAGAGATCG GAGAGTCATC ATTTGGACTC TAATGGAACC 
AGTGGTTTAT ATGGGCAACA TACCACAAAG AAGCCAAAGA TATCTAAGCA ATTGCTAGAT 
AATACTTTTG ACAACATGGT TCAAATGACC GGGTCCATCC CATCTCCAGC TGCTTCCCAG 
ATGAGTAATA TGTCAAACAC AAATAGATTC ATCAAATTGA TTGGTGGCAG GGAACGAGGC 
AGAAAAAATA AATCAATGAA GATGTCTGTT GGGCAGCCAG GTTCTGGAAG TCCCTGGTCA 
CTTTTTGAAG ACCAGGCTCT TGTTGTTCTT GTGCATGACA TGGGTCCTAA TTGGGAGCTT 
ATAAGCGATG CTATCAACAG CACTGCTCAA TTCAAGTGTA TATTCCGCAA GCCTAAAGAA 
TGCAAAGATC GTCACAAAAT CTTAATGGAT AAGGGTGCTG GTGATGGGGC TGATAGTGCT 
GAGGATTCAG GATCTTCTCA ATCCTACCCA TCAACTTTGC CTGGCATTCC AAAGCAGGGC 
AGTGCCAGAC AGTTGTTTCA ACATTTGCAA GGGCCGATGC AAGAGGATAC CCTGAAGTCT 
CATTTTGAGA AAATCATCAT TATTGGGAAG AAACATCATT ACAAAAGGAG TCAGAATGAA 
AACCAGGATC CCAAGCAAAT AGCAGCAACT CACAATTCTC ATTTTATTGC TCTTTCCCAA 
GTTTGCCCAA ACAACTTAAA TGGAGGTGTC CTAACGCCCC TTGATCTCTG TGATTCAAGC 
ACTTCAAATC CAGATGTTCT TCCCAATGTG TATCAAGGTT CTCATGCTAG CAATTTGGTA 
ATGCCAAACC AAGGTGCTGT TGCATCAACG CTTCCCACTT CTGGTGCAAT CTCTTCTCTG 
CAAGGATCTT CTGGCGTGGT TCTTGGCAAT AACTCATCAT CACCATCTGG TCCCCTCAAT 
GCACCTCATA GAGATGGTAG ATACAATGTT CCAAGGACAT CTTTACCAGT TGACGAGCAT 
CAAAGAATGC AACCCTATCA AATGTTACCT AGTAGAAACT TGCAGCAGTC TAACATGTCG 
GTTTCTGGGG CTGTCTCTGG AGCTGATCGT GGTGTTCGCA TGTTATCGAG TGGAAATGGT 
ATGGGCATGA TGCCAGGGAT GAACAGAAGC ATGCCACTGC CACGGTCAGG ATTTCAAGGA 
ACAGCCTCTT CATCAATGCT GAATTCTGGA AGTATGCTTT CTAACAATGT GGTTGGGATG 
TCAAGCCCTG TGAATATGCA CACTGGTTCT GGTCAAGGGA ACTTGATGAG ACCTCGCGAG 
GCTTTGCATA TGTTGAGGCT TGGCCATAAT CATGAGCATC AAAGACAAAT GATGGTACCA 
GAGCTTCAGA TGCAGCCAAC ACAAGGGAAC AACCAAGGAA TTTCTGCTTT CAACGGGGTG 
CCTACTGCTT TTGCTAATCA GACAACTACT TCACCTGTGC AGACATATCC AGGCCATCCC 
CAGCAGCAGC ATCAAATGCC TGCACAACAG TCCAATATGC TTAGCAATCC TCATCATCCT 
AATCTCCGTG GCCCCAATCA GGCTACAGGT TCACAGCATC CAGCATATGC TATGCACCTG 
CCTAAAGGAA GGCAAATGCA GCAGCGGCTT CTCCAGCAGC AGCAGCAGCA GCAGCAGCAC 
TTTTCTGCAT CTAGTGCTTT GATGCCCCAT GTCCAACATC AATCCCAACT TCCCATATCA 
TCGTCTATGC AAAATAGTTC TCAAATCTCA CCACCATCTG CATCGCAGCC GGTGTCACTA 
CCTGCTATAA CTCCCCCTTC CCCAATGACT CCCATTTCAA TGCAGCAGCA ACAGCAGCAG 
AAACACAACT TGCCACATCA TGCGGTCAGT CGGAGCCCCC AAAGTGGTTC CAGTGGGTTG 
ACCAATCAGA TGGGAAAGCA GCGACAACGG CAACCACAGC AGTTTCAACA ATCTGGCAGA 
CATCACCCTC AACAACGACA ACACTCACAG TCTCCACAGC AGGCTAAACT TTTGAAGGGA 
ATGGGGAGAG GGAACATGGT GGTGCACCAG AACCTTCCCA ATGATCACTC TCCTTTGAAT 
GGCCTTTCTG TGCCTCCAGG AAACCATGGT GTAGAGAAAG GAGAGCAAAT CATGCACTTG 
ATGCAGGGTC AAGGCTTGTA TTCTGGGACT GGGTTGAGTC CAATTCATAC ATCTAAACCA 
TTGGGTCCTT CACAATCTCC AAACCATTCT CAGCCACAGC AAAAGCTATA TTCGGGCCCT 
ACAACCCCTT CATCAAAGCC ACTCCAGCAA ATGCCTTCTC ATTTGGAAAG TAGTACTCAA 
GGCCAGGTCC AACCAGTACC CTCTGGTCAA ACACTAACAG CTACTCACCA GAACACTCCA 
GTTATGGTTC CCAGTCATCA GCATCTTCAA CAACATCCAC AGCCACATCA GAAGCAGGTT 
AGTCAACCTC AACCAACTGT TCAGAGAATG CTTCAACAGA GTCGTCTGTT GAATTCTGAT 
CTGCCAACAA AGCCTCAAAC TGATCAGGGC CATGCTGATC AGCAAACTTC AAACAACATC 
TCCCAGACAG GAACAAGTAC ATCCACAGGG ATGCCTTTGG CCTGTAATGA TACATCTAAT 
GTGGCACCAG TTGTTTCTTC TGTTTCTGAG ATGCAGTGGA AATCATCAGA ACCATCCTTG 
CATGATTCTG GCATGGCAGA TACAGCTTCT AAGGTAGGTC CTATTGGTAG CCCCCCCCTT 
ACGAGTGCAG CTGGGAGTGA GCAAGTGGTG CCTGTCAGCC AAGGTTCAGT CCACAGGCAG 
TTATCAGGAG GCTTGCCCCT GCATTGCCAT AATGGTGGGA CACGGTGGCA GCACCAACAA 
CCACATCAAT CAACAGCATC GCCTTCCCCA TGCCAGCAAC TTTTGCAGTC ACAAGAGCCA 
CAACTACAGC AAGAACGACA ACAATCACCT CAACAGCTGC CACCACAGCA GCCGACTCAG 
AAGCAGACAC AGCATCTTCA ATCAGGACAA GGCAATTTAT ACATCACGCC AAGCAATTCT 
GGTCTGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024451339.1
CDS	401..6472
Translation	MHGCGSGYALLVNAEVDSMGGVVDGGVGIATKTSPRRAAIEKAQVELRQEYDVREERRRELEFLEKGGNPLDFKFVNATSVSVQSTSLTDHHVEQFVTSEAKGSFPLTASPHGDSVESSGRPGATPVCEPNSADNFDGENELLEVERKRKHPSRRNNVTQSEQSSQMDGIHNAKESEDSAIFRPYARRNRSRPNRDGARSSSTDIVQSSVGHGSSLPARGDARDVKGLVTETDDHKAQIITSISNPKSTTSNGDLFFQIDTSNTQSNTELDCVQALKTVVNLPDDRLDVTESIVLRDNQHDQPSEADAEKAPNDIASRECDHGGGKELVISAGPECPPCAESAKTENETGPALLNGLEKDGNEGQNGNAAMGTERFNSESSCTQNSLSLDANNGCDPCDNRRNDDTNEILLKESSEFEGTRSLPSGNIGNEKKETNSISAINDGSVHENYSGNDSTVKNEEERRTTFHSQVKCTNLEGVEQNDHVASEADTKAGNMLADSSNSIREIIYPSGPQGSLDPPVQELPQPILLEKNSFVATDPQSCSNTHVKVVDKSHEDSILEEARVIEAKRKRIAELSVASVHSENRRRSHWDFVLEEMAWLANDVAQERLWKMTAAAQICRRIAFTSRLRVEEQNHHLKLKNVAYSLAKAVMQFWHSAKVYLSNNCHSVGSKNGKHEVGMFVGNEFSVNKFGDIDKEQVACKELEKQNPAKNIAHSIHGYAVRFLKYNSSPFPSFQAEAPATPDRIADLGIVDTSWDDRLTEESLFYAVPSGAMAMYRLSIESHIAQSEKTRSSMQEEVDTSMYDTPADFGYHDTAAYDEEEGETSAYYMHGVFEGSKSAKHDQKKRKSLTKSPSARSYDLGTDSPYGHCTTGPQQNVLMGKRPASNLNAGSIPTKRMRTASRQRFTSPFTAGTAGVLLQAPVKTDASSGDTNSFQDDQSILHGGSQIQKSVEVESAAHFERQLPYDYAETSTKPKKKKKAKHLGSAYEQGWQLDSTGHNEQRDNFKKRSESHHLDSNGTSGLYGQHTTKKPKISKQLLDNTFDNMVQMTGSIPSPAASQMSNMSNTNRFIKLIGGRERGRKNKSMKMSVGQPGSGSPWSLFEDQALVVLVHDMGPNWELISDAINSTAQFKCIFRKPKECKDRHKILMDKGAGDGADSAEDSGSSQSYPSTLPGIPKQGSARQLFQHLQGPMQEDTLKSHFEKIIIIGKKHHYKRSQNENQDPKQIAATHNSHFIALSQVCPNNLNGGVLTPLDLCDSSTSNPDVLPNVYQGSHASNLVMPNQGAVASTLPTSGAISSLQGSSGVVLGNNSSSPSGPLNAPHRDGRYNVPRTSLPVDEHQRMQPYQMLPSRNLQQSNMSVSGAVSGADRGVRMLSSGNGMGMMPGMNRSMPLPRSGFQGTASSSMLNSGSMLSNNVVGMSSPVNMHTGSGQGNLMRPREALHMLRLGHNHEHQRQMMVPELQMQPTQGNNQGISAFNGVPTAFANQTTTSPVQTYPGHPQQQHQMPAQQSNMLSNPHHPNLRGPNQATGSQHPAYAMHLPKGRQMQQRLLQQQQQQQQHFSASSALMPHVQHQSQLPISSSMQNSSQISPPSASQPVSLPAITPPSPMTPISMQQQQQQKHNLPHHAVSRSPQSGSSGLTNQMGKQRQRQPQQFQQSGRHHPQQRQHSQSPQQAKLLKGMGRGNMVVHQNLPNDHSPLNGLSVPPGNHGVEKGEQIMHLMQGQGLYSGTGLSPIHTSKPLGPSQSPNHSQPQQKLYSGPTTPSSKPLQQMPSHLESSTQGQVQPVPSGQTLTATHQNTPVMVPSHQHLQQHPQPHQKQVSQPQPTVQRMLQQSRLLNSDLPTKPQTDQGHADQQTSNNISQTGTSTSTGMPLACNDTSNVAPVVSSVSEMQWKSSEPSLHDSGMADTASKVGPIGSPPLTSAAGSEQVVPVSQGSVHRQLSGGLPLHCHNGGTRWQHQQPHQSTASPSPCQQLLQSQEPQLQQERQQSPQQLPPQQPTQKQTQHLQSGQGNLYITPSNSGLE

Target ORF information:

RefSeq Version	XM_024595571.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa chromatin modification-related protein EAF1 B (LOC7459484), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024595571.1

ORF Insert Sequence:

ATGCATGGAT GTGGTTCAGG ATATGCTCTC CTAGTAAATG CTGAGGTTGA TTCCATGGGT 
GGGGTTGTTG ATGGTGGAGT TGGTATTGCT ACCAAAACCT CTCCGCGGCG AGCAGCAATT 
GAGAAGGCCC AGGTGGAACT TAGACAAGAG TATGATGTTC GTGAGGAACG GAGAAGGGAG 
CTAGAATTTC TTGAGAAAGG CGGCAATCCT TTGGATTTTA AATTTGTAAA TGCAACTTCG 
GTCAGTGTCC AGTCTACTTC ACTCACAGAT CATCACGTTG AACAGTTTGT TACTAGTGAA 
GCAAAAGGCA GTTTTCCACT GACTGCATCA CCTCATGGGG ATTCTGTAGA AAGTAGTGGC 
AGACCAGGGG CTACTCCAGT TTGTGAGCCC AATAGTGCTG ACAATTTTGA TGGTGAAAAT 
GAGTTACTTG AAGTTGAAAG GAAACGTAAA CATCCTAGTA GGAGGAATAA TGTCACTCAA 
TCAGAGCAGT CTTCACAGAT GGATGGAATT CACAATGCCA AGGAATCAGA AGATTCTGCT 
ATTTTTCGGC CATATGCTCG AAGGAATAGG TCCAGACCAA ATCGTGACGG CGCGCGATCA 
AGTTCAACTG ATATTGTTCA GAGTTCAGTT GGCCATGGAT CCTCTTTACC GGCTCGTGGG 
GATGCAAGGG ATGTGAAAGG GTTGGTAACT GAAACAGACG ATCATAAAGC CCAGATTATT 
ACTTCCATCT CTAATCCAAA GTCCACCACT TCAAACGGTG ACCTGTTTTT TCAGATTGAC 
ACTTCCAATA CACAGTCAAA CACGGAGCTT GATTGTGTGC AGGCTCTGAA AACAGTAGTC 
AACCTGCCTG ATGATAGGTT GGATGTTACA GAGTCCATTG TCTTGAGGGA TAACCAACAT 
GACCAGCCTT CAGAAGCTGA TGCTGAGAAA GCACCCAATG ATATTGCTTC CAGGGAATGC 
GATCATGGTG GAGGAAAAGA GCTGGTTATT TCAGCTGGTC CTGAATGTCC CCCTTGTGCT 
GAATCAGCCA AGACTGAGAA TGAAACTGGT CCTGCTCTGC TCAATGGCTT GGAAAAAGAT 
GGAAATGAAG GTCAAAATGG TAATGCAGCA ATGGGAACGG AACGATTCAA TTCAGAGTCC 
TCTTGCACCC AAAATAGCTT AAGCCTGGAT GCAAACAATG GCTGTGATCC TTGTGATAAT 
CGTAGAAATG ATGACACCAA TGAAATTCTT TTGAAAGAAT CATCAGAATT TGAAGGAACA 
CGAAGTTTAC CATCGGGTAA TATAGGAAAT GAAAAGAAAG AGACTAACTC GATCTCTGCA 
ATCAATGATG GTTCTGTGCA TGAAAACTAC TCTGGCAATG ATTCTACTGT CAAAAATGAG 
GAAGAAAGGA GAACAACTTT TCACTCTCAG GTGAAGTGTA CTAATCTTGA AGGAGTGGAA 
CAAAATGACC ATGTTGCCTC TGAGGCTGAT ACAAAGGCGG GCAACATGTT GGCTGATAGT 
TCTAATTCCA TCAGGGAAAT AATTTACCCC AGTGGTCCCC AGGGCTCTTT GGATCCTCCT 
GTTCAAGAGC TTCCCCAGCC TATTTTGTTA GAGAAAAACT CTTTTGTTGC CACTGATCCT 
CAATCTTGTT CGAATACACA TGTTAAAGTG GTAGACAAGT CTCATGAAGA TTCTATTTTG 
GAAGAGGCAC GGGTTATAGA GGCTAAGCGC AAGAGGATTG CAGAATTATC TGTTGCATCT 
GTGCATTCAG AGAATCGCCG GAGATCTCAC TGGGATTTTG TCCTTGAAGA AATGGCATGG 
CTGGCAAATG ATGTTGCACA GGAGCGTCTC TGGAAGATGA CTGCTGCTGC TCAAATATGT 
CGACGAATTG CTTTTACTTC TCGATTGAGA GTTGAAGAGC AGAATCATCA TTTGAAGCTA 
AAAAACGTCG CTTACAGTCT TGCTAAGGCT GTCATGCAGT TCTGGCATTC AGCAAAGGTG 
TATCTAAGTA ACAATTGTCA CAGTGTTGGT TCAAAGAATG GGAAGCATGA GGTGGGGATG 
TTTGTTGGGA ATGAATTTTC TGTCAACAAG TTTGGAGATA TTGATAAGGA GCAGGTGGCA 
TGCAAAGAGT TAGAGAAACA AAATCCTGCC AAAAACATTG CGCATTCCAT TCATGGATAT 
GCTGTAAGGT TTTTGAAATA TAACAGTTCT CCATTTCCTT CCTTTCAAGC AGAAGCACCA 
GCAACTCCTG ATAGAATAGC TGATTTGGGC ATTGTGGACA CTTCATGGGA TGATCGCCTT 
ACTGAAGAAA GCCTGTTCTA CGCAGTTCCC TCAGGTGCCA TGGCAATGTA CAGATTGTCC 
ATTGAATCTC ATATTGCGCA GAGTGAGAAA ACTCGCAGTA GCATGCAGGA GGAAGTCGAT 
ACATCCATGT ATGACACTCC AGCAGATTTT GGATATCATG ATACTGCTGC ATACGATGAG 
GAGGAAGGAG AAACAAGTGC TTACTATATG CATGGAGTCT TTGAAGGTAG CAAGTCAGCA 
AAACATGACC AAAAGAAACG GAAAAGCTTA ACAAAGTCAC CCTCTGCAAG ATCTTATGAT 
CTGGGAACTG ATTCACCTTA TGGACACTGC ACCACTGGGC CACAACAAAA TGTATTGATG 
GGGAAAAGAC CTGCTAGTAA TCTTAATGCT GGCTCAATCC CAACAAAACG CATGCGTACT 
GCTTCTAGAC AGAGGTTTAC AAGTCCTTTT ACTGCTGGAA CTGCTGGGGT TTTACTCCAG 
GCTCCAGTGA AGACAGATGC ATCGAGTGGA GATACCAATT CTTTTCAGGA TGATCAGAGT 
ATTTTGCATG GTGGGTCACA AATTCAGAAA AGTGTGGAGG TTGAGTCCGC TGCGCATTTC 
GAAAGGCAGT TACCATATGA CTACGCAGAG ACATCAACAA AACCGAAGAA GAAGAAGAAG 
GCAAAGCACC TGGGTTCTGC TTATGAGCAG GGGTGGCAGT TGGATTCTAC TGGTCATAAT 
GAACAGAGGG ATAATTTCAA AAAGAGATCG GAGAGTCATC ATTTGGACTC TAATGGAACC 
AGTGGTTTAT ATGGGCAACA TACCACAAAG AAGCCAAAGA TATCTAAGCA ATTGCTAGAT 
AATACTTTTG ACAACATGGT TCAAATGACC GGGTCCATCC CATCTCCAGC TGCTTCCCAG 
ATGAGTAATA TGTCAAACAC AAATAGATTC ATCAAATTGA TTGGTGGCAG GGAACGAGGC 
AGAAAAAATA AATCAATGAA GATGTCTGTT GGGCAGCCAG GTTCTGGAAG TCCCTGGTCA 
CTTTTTGAAG ACCAGGCTCT TGTTGTTCTT GTGCATGACA TGGGTCCTAA TTGGGAGCTT 
ATAAGCGATG CTATCAACAG CACTGCTCAA TTCAAGTGTA TATTCCGCAA GCCTAAAGAA 
TGCAAAGATC GTCACAAAAT CTTAATGGAT AAGGGTGCTG GTGATGGGGC TGATAGTGCT 
GAGGATTCAG GATCTTCTCA ATCCTACCCA TCAACTTTGC CTGGCATTCC AAAGCAGGGC 
AGTGCCAGAC AGTTGTTTCA ACATTTGCAA GGGCCGATGC AAGAGGATAC CCTGAAGTCT 
CATTTTGAGA AAATCATCAT TATTGGGAAG AAACATCATT ACAAAAGGAG TCAGAATGAA 
AACCAGGATC CCAAGCAAAT AGCAGCAACT CACAATTCTC ATTTTATTGC TCTTTCCCAA 
GTTTGCCCAA ACAACTTAAA TGGAGGTGTC CTAACGCCCC TTGATCTCTG TGATTCAAGC 
ACTTCAAATC CAGATGTTCT TCCCAATGTG TATCAAGGTT CTCATGCTAG CAATTTGGTA 
ATGCCAAACC AAGGTGCTGT TGCATCAACG CTTCCCACTT CTGGTGCAAT CTCTTCTCTG 
CAAGGATCTT CTGGCGTGGT TCTTGGCAAT AACTCATCAT CACCATCTGG TCCCCTCAAT 
GCACCTCATA GAGATGGTAG ATACAATGTT CCAAGGACAT CTTTACCAGT TGACGAGCAT 
CAAAGAATGC AACCCTATCA AATGTTACCT AGTAGAAACT TGCAGCAGTC TAACATGTCG 
GTTTCTGGGG CTGTCTCTGG AGCTGATCGT GGTGTTCGCA TGTTATCGAG TGGAAATGGT 
ATGGGCATGA TGCCAGGGAT GAACAGAAGC ATGCCACTGC CACGGTCAGG ATTTCAAGGA 
ACAGCCTCTT CATCAATGCT GAATTCTGGA AGTATGCTTT CTAACAATGT GGTTGGGATG 
TCAAGCCCTG TGAATATGCA CACTGGTTCT GGTCAAGGGA ACTTGATGAG ACCTCGCGAG 
GCTTTGCATA TGTTGAGGCT TGGCCATAAT CATGAGCATC AAAGACAAAT GATGGTACCA 
GAGCTTCAGA TGCAGCCAAC ACAAGGGAAC AACCAAGGAA TTTCTGCTTT CAACGGGGTG 
CCTACTGCTT TTGCTAATCA GACAACTACT TCACCTGTGC AGACATATCC AGGCCATCCC 
CAGCAGCAGC ATCAAATGCC TGCACAACAG TCCAATATGC TTAGCAATCC TCATCATCCT 
AATCTCCGTG GCCCCAATCA GGCTACAGGT TCACAGCATC CAGCATATGC TATGCACCTG 
CCTAAAGGAA GGCAAATGCA GCAGCGGCTT CTCCAGCAGC AGCAGCAGCA GCAGCAGCAC 
TTTTCTGCAT CTAGTGCTTT GATGCCCCAT GTCCAACATC AATCCCAACT TCCCATATCA 
TCGTCTATGC AAAATAGTTC TCAAATCTCA CCACCATCTG CATCGCAGCC GGTGTCACTA 
CCTGCTATAA CTCCCCCTTC CCCAATGACT CCCATTTCAA TGCAGCAGCA ACAGCAGCAG 
AAACACAACT TGCCACATCA TGCGGTCAGT CGGAGCCCCC AAAGTGGTTC CAGTGGGTTG 
ACCAATCAGA TGGGAAAGCA GCGACAACGG CAACCACAGC AGTTTCAACA ATCTGGCAGA 
CATCACCCTC AACAACGACA ACACTCACAG TCTCCACAGC AGGCTAAACT TTTGAAGGGA 
ATGGGGAGAG GGAACATGGT GGTGCACCAG AACCTTCCCA ATGATCACTC TCCTTTGAAT 
GGCCTTTCTG TGCCTCCAGG AAACCATGGT GTAGAGAAAG GAGAGCAAAT CATGCACTTG 
ATGCAGGGTC AAGGCTTGTA TTCTGGGACT GGGTTGAGTC CAATTCATAC ATCTAAACCA 
TTGGGTCCTT CACAATCTCC AAACCATTCT CAGCCACAGC AAAAGCTATA TTCGGGCCCT 
ACAACCCCTT CATCAAAGCC ACTCCAGCAA ATGCCTTCTC ATTTGGAAAG TAGTACTCAA 
GGCCAGGTCC AACCAGTACC CTCTGGTCAA ACACTAACAG CTACTCACCA GAACACTCCA 
GTTATGGTTC CCAGTCATCA GCATCTTCAA CAACATCCAC AGCCACATCA GAAGCAGGTT 
AGTCAACCTC AACCAACTGT TCAGAGAATG CTTCAACAGA GTCGTCTGTT GAATTCTGAT 
CTGCCAACAA AGCCTCAAAC TGATCAGGGC CATGCTGATC AGCAAACTTC AAACAACATC 
TCCCAGACAG GAACAAGTAC ATCCACAGGG ATGCCTTTGG CCTGTAATGA TACATCTAAT 
GTGGCACCAG TTGTTTCTTC TGTTTCTGAG ATGCAGTGGA AATCATCAGA ACCATCCTTG 
CATGATTCTG GCATGGCAGA TACAGCTTCT AAGGTAGGTC CTATTGGTAG CCCCCCCCTT 
ACGAGTGCAG CTGGGAGTGA GCAAGTGGTG CCTGTCAGCC AAGGTTCAGT CCACAGGCAG 
TTATCAGGAG GCTTGCCCCT GCATTGCCAT AATGGTGGGA CACGGTGGCA GCACCAACAA 
CCACATCAAT CAACAGCATC GCCTTCCCCA TGCCAGCAAC TTTTGCAGTC ACAAGAGCCA 
CAACTACAGC AAGAACGACA ACAATCACCT CAACAGCTGC CACCACAGCA GCCGACTCAG 
AAGCAGACAC AGCATCTTCA ATCAGGACAA GGCAATTTAT ACATCACGCC AAGCAATTCT 
GGTCTGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7459484 ( XM_024595572.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024451340.1 Populus trichocarpa chromatin modification-related protein EAF1 B (LOC7459484), transcript variant X2, mRNA.

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CloneID	OPa424908
Clone ID Related Accession (Same CDS sequence)	XM_024595574.1 , XM_024595573.1 , XM_024595571.1 , XM_024595572.1
Accession Version	XM_024595572.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	chromatin modification-related protein EAF1 B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037286.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGGAT GTGGTTCAGG ATATGCTCTC CTAGTAAATG CTGAGGTTGA TTCCATGGGT 
GGGGTTGTTG ATGGTGGAGT TGGTATTGCT ACCAAAACCT CTCCGCGGCG AGCAGCAATT 
GAGAAGGCCC AGGTGGAACT TAGACAAGAG TATGATGTTC GTGAGGAACG GAGAAGGGAG 
CTAGAATTTC TTGAGAAAGG CGGCAATCCT TTGGATTTTA AATTTGTAAA TGCAACTTCG 
GTCAGTGTCC AGTCTACTTC ACTCACAGAT CATCACGTTG AACAGTTTGT TACTAGTGAA 
GCAAAAGGCA GTTTTCCACT GACTGCATCA CCTCATGGGG ATTCTGTAGA AAGTAGTGGC 
AGACCAGGGG CTACTCCAGT TTGTGAGCCC AATAGTGCTG ACAATTTTGA TGGTGAAAAT 
GAGTTACTTG AAGTTGAAAG GAAACGTAAA CATCCTAGTA GGAGGAATAA TGTCACTCAA 
TCAGAGCAGT CTTCACAGAT GGATGGAATT CACAATGCCA AGGAATCAGA AGATTCTGCT 
ATTTTTCGGC CATATGCTCG AAGGAATAGG TCCAGACCAA ATCGTGACGG CGCGCGATCA 
AGTTCAACTG ATATTGTTCA GAGTTCAGTT GGCCATGGAT CCTCTTTACC GGCTCGTGGG 
GATGCAAGGG ATGTGAAAGG GTTGGTAACT GAAACAGACG ATCATAAAGC CCAGATTATT 
ACTTCCATCT CTAATCCAAA GTCCACCACT TCAAACGGTG ACCTGTTTTT TCAGATTGAC 
ACTTCCAATA CACAGTCAAA CACGGAGCTT GATTGTGTGC AGGCTCTGAA AACAGTAGTC 
AACCTGCCTG ATGATAGGTT GGATGTTACA GAGTCCATTG TCTTGAGGGA TAACCAACAT 
GACCAGCCTT CAGAAGCTGA TGCTGAGAAA GCACCCAATG ATATTGCTTC CAGGGAATGC 
GATCATGGTG GAGGAAAAGA GCTGGTTATT TCAGCTGGTC CTGAATGTCC CCCTTGTGCT 
GAATCAGCCA AGACTGAGAA TGAAACTGGT CCTGCTCTGC TCAATGGCTT GGAAAAAGAT 
GGAAATGAAG GTCAAAATGG TAATGCAGCA ATGGGAACGG AACGATTCAA TTCAGAGTCC 
TCTTGCACCC AAAATAGCTT AAGCCTGGAT GCAAACAATG GCTGTGATCC TTGTGATAAT 
CGTAGAAATG ATGACACCAA TGAAATTCTT TTGAAAGAAT CATCAGAATT TGAAGGAACA 
CGAAGTTTAC CATCGGGTAA TATAGGAAAT GAAAAGAAAG AGACTAACTC GATCTCTGCA 
ATCAATGATG GTTCTGTGCA TGAAAACTAC TCTGGCAATG ATTCTACTGT CAAAAATGAG 
GAAGAAAGGA GAACAACTTT TCACTCTCAG GTGAAGTGTA CTAATCTTGA AGGAGTGGAA 
CAAAATGACC ATGTTGCCTC TGAGGCTGAT ACAAAGGCGG GCAACATGTT GGCTGATAGT 
TCTAATTCCA TCAGGGAAAT AATTTACCCC AGTGGTCCCC AGGGCTCTTT GGATCCTCCT 
GTTCAAGAGC TTCCCCAGCC TATTTTGTTA GAGAAAAACT CTTTTGTTGC CACTGATCCT 
CAATCTTGTT CGAATACACA TGTTAAAGTG GTAGACAAGT CTCATGAAGA TTCTATTTTG 
GAAGAGGCAC GGGTTATAGA GGCTAAGCGC AAGAGGATTG CAGAATTATC TGTTGCATCT 
GTGCATTCAG AGAATCGCCG GAGATCTCAC TGGGATTTTG TCCTTGAAGA AATGGCATGG 
CTGGCAAATG ATGTTGCACA GGAGCGTCTC TGGAAGATGA CTGCTGCTGC TCAAATATGT 
CGACGAATTG CTTTTACTTC TCGATTGAGA GTTGAAGAGC AGAATCATCA TTTGAAGCTA 
AAAAACGTCG CTTACAGTCT TGCTAAGGCT GTCATGCAGT TCTGGCATTC AGCAAAGGTG 
TATCTAAGTA ACAATTGTCA CAGTGTTGGT TCAAAGAATG GGAAGCATGA GGTGGGGATG 
TTTGTTGGGA ATGAATTTTC TGTCAACAAG TTTGGAGATA TTGATAAGGA GCAGGTGGCA 
TGCAAAGAGT TAGAGAAACA AAATCCTGCC AAAAACATTG CGCATTCCAT TCATGGATAT 
GCTGTAAGGT TTTTGAAATA TAACAGTTCT CCATTTCCTT CCTTTCAAGC AGAAGCACCA 
GCAACTCCTG ATAGAATAGC TGATTTGGGC ATTGTGGACA CTTCATGGGA TGATCGCCTT 
ACTGAAGAAA GCCTGTTCTA CGCAGTTCCC TCAGGTGCCA TGGCAATGTA CAGATTGTCC 
ATTGAATCTC ATATTGCGCA GAGTGAGAAA ACTCGCAGTA GCATGCAGGA GGAAGTCGAT 
ACATCCATGT ATGACACTCC AGCAGATTTT GGATATCATG ATACTGCTGC ATACGATGAG 
GAGGAAGGAG AAACAAGTGC TTACTATATG CATGGAGTCT TTGAAGGTAG CAAGTCAGCA 
AAACATGACC AAAAGAAACG GAAAAGCTTA ACAAAGTCAC CCTCTGCAAG ATCTTATGAT 
CTGGGAACTG ATTCACCTTA TGGACACTGC ACCACTGGGC CACAACAAAA TGTATTGATG 
GGGAAAAGAC CTGCTAGTAA TCTTAATGCT GGCTCAATCC CAACAAAACG CATGCGTACT 
GCTTCTAGAC AGAGGTTTAC AAGTCCTTTT ACTGCTGGAA CTGCTGGGGT TTTACTCCAG 
GCTCCAGTGA AGACAGATGC ATCGAGTGGA GATACCAATT CTTTTCAGGA TGATCAGAGT 
ATTTTGCATG GTGGGTCACA AATTCAGAAA AGTGTGGAGG TTGAGTCCGC TGCGCATTTC 
GAAAGGCAGT TACCATATGA CTACGCAGAG ACATCAACAA AACCGAAGAA GAAGAAGAAG 
GCAAAGCACC TGGGTTCTGC TTATGAGCAG GGGTGGCAGT TGGATTCTAC TGGTCATAAT 
GAACAGAGGG ATAATTTCAA AAAGAGATCG GAGAGTCATC ATTTGGACTC TAATGGAACC 
AGTGGTTTAT ATGGGCAACA TACCACAAAG AAGCCAAAGA TATCTAAGCA ATTGCTAGAT 
AATACTTTTG ACAACATGGT TCAAATGACC GGGTCCATCC CATCTCCAGC TGCTTCCCAG 
ATGAGTAATA TGTCAAACAC AAATAGATTC ATCAAATTGA TTGGTGGCAG GGAACGAGGC 
AGAAAAAATA AATCAATGAA GATGTCTGTT GGGCAGCCAG GTTCTGGAAG TCCCTGGTCA 
CTTTTTGAAG ACCAGGCTCT TGTTGTTCTT GTGCATGACA TGGGTCCTAA TTGGGAGCTT 
ATAAGCGATG CTATCAACAG CACTGCTCAA TTCAAGTGTA TATTCCGCAA GCCTAAAGAA 
TGCAAAGATC GTCACAAAAT CTTAATGGAT AAGGGTGCTG GTGATGGGGC TGATAGTGCT 
GAGGATTCAG GATCTTCTCA ATCCTACCCA TCAACTTTGC CTGGCATTCC AAAGCAGGGC 
AGTGCCAGAC AGTTGTTTCA ACATTTGCAA GGGCCGATGC AAGAGGATAC CCTGAAGTCT 
CATTTTGAGA AAATCATCAT TATTGGGAAG AAACATCATT ACAAAAGGAG TCAGAATGAA 
AACCAGGATC CCAAGCAAAT AGCAGCAACT CACAATTCTC ATTTTATTGC TCTTTCCCAA 
GTTTGCCCAA ACAACTTAAA TGGAGGTGTC CTAACGCCCC TTGATCTCTG TGATTCAAGC 
ACTTCAAATC CAGATGTTCT TCCCAATGTG TATCAAGGTT CTCATGCTAG CAATTTGGTA 
ATGCCAAACC AAGGTGCTGT TGCATCAACG CTTCCCACTT CTGGTGCAAT CTCTTCTCTG 
CAAGGATCTT CTGGCGTGGT TCTTGGCAAT AACTCATCAT CACCATCTGG TCCCCTCAAT 
GCACCTCATA GAGATGGTAG ATACAATGTT CCAAGGACAT CTTTACCAGT TGACGAGCAT 
CAAAGAATGC AACCCTATCA AATGTTACCT AGTAGAAACT TGCAGCAGTC TAACATGTCG 
GTTTCTGGGG CTGTCTCTGG AGCTGATCGT GGTGTTCGCA TGTTATCGAG TGGAAATGGT 
ATGGGCATGA TGCCAGGGAT GAACAGAAGC ATGCCACTGC CACGGTCAGG ATTTCAAGGA 
ACAGCCTCTT CATCAATGCT GAATTCTGGA AGTATGCTTT CTAACAATGT GGTTGGGATG 
TCAAGCCCTG TGAATATGCA CACTGGTTCT GGTCAAGGGA ACTTGATGAG ACCTCGCGAG 
GCTTTGCATA TGTTGAGGCT TGGCCATAAT CATGAGCATC AAAGACAAAT GATGGTACCA 
GAGCTTCAGA TGCAGCCAAC ACAAGGGAAC AACCAAGGAA TTTCTGCTTT CAACGGGGTG 
CCTACTGCTT TTGCTAATCA GACAACTACT TCACCTGTGC AGACATATCC AGGCCATCCC 
CAGCAGCAGC ATCAAATGCC TGCACAACAG TCCAATATGC TTAGCAATCC TCATCATCCT 
AATCTCCGTG GCCCCAATCA GGCTACAGGT TCACAGCATC CAGCATATGC TATGCACCTG 
CCTAAAGGAA GGCAAATGCA GCAGCGGCTT CTCCAGCAGC AGCAGCAGCA GCAGCAGCAC 
TTTTCTGCAT CTAGTGCTTT GATGCCCCAT GTCCAACATC AATCCCAACT TCCCATATCA 
TCGTCTATGC AAAATAGTTC TCAAATCTCA CCACCATCTG CATCGCAGCC GGTGTCACTA 
CCTGCTATAA CTCCCCCTTC CCCAATGACT CCCATTTCAA TGCAGCAGCA ACAGCAGCAG 
AAACACAACT TGCCACATCA TGCGGTCAGT CGGAGCCCCC AAAGTGGTTC CAGTGGGTTG 
ACCAATCAGA TGGGAAAGCA GCGACAACGG CAACCACAGC AGTTTCAACA ATCTGGCAGA 
CATCACCCTC AACAACGACA ACACTCACAG TCTCCACAGC AGGCTAAACT TTTGAAGGGA 
ATGGGGAGAG GGAACATGGT GGTGCACCAG AACCTTCCCA ATGATCACTC TCCTTTGAAT 
GGCCTTTCTG TGCCTCCAGG AAACCATGGT GTAGAGAAAG GAGAGCAAAT CATGCACTTG 
ATGCAGGGTC AAGGCTTGTA TTCTGGGACT GGGTTGAGTC CAATTCATAC ATCTAAACCA 
TTGGGTCCTT CACAATCTCC AAACCATTCT CAGCCACAGC AAAAGCTATA TTCGGGCCCT 
ACAACCCCTT CATCAAAGCC ACTCCAGCAA ATGCCTTCTC ATTTGGAAAG TAGTACTCAA 
GGCCAGGTCC AACCAGTACC CTCTGGTCAA ACACTAACAG CTACTCACCA GAACACTCCA 
GTTATGGTTC CCAGTCATCA GCATCTTCAA CAACATCCAC AGCCACATCA GAAGCAGGTT 
AGTCAACCTC AACCAACTGT TCAGAGAATG CTTCAACAGA GTCGTCTGTT GAATTCTGAT 
CTGCCAACAA AGCCTCAAAC TGATCAGGGC CATGCTGATC AGCAAACTTC AAACAACATC 
TCCCAGACAG GAACAAGTAC ATCCACAGGG ATGCCTTTGG CCTGTAATGA TACATCTAAT 
GTGGCACCAG TTGTTTCTTC TGTTTCTGAG ATGCAGTGGA AATCATCAGA ACCATCCTTG 
CATGATTCTG GCATGGCAGA TACAGCTTCT AAGGTAGGTC CTATTGGTAG CCCCCCCCTT 
ACGAGTGCAG CTGGGAGTGA GCAAGTGGTG CCTGTCAGCC AAGGTTCAGT CCACAGGCAG 
TTATCAGGAG GCTTGCCCCT GCATTGCCAT AATGGTGGGA CACGGTGGCA GCACCAACAA 
CCACATCAAT CAACAGCATC GCCTTCCCCA TGCCAGCAAC TTTTGCAGTC ACAAGAGCCA 
CAACTACAGC AAGAACGACA ACAATCACCT CAACAGCTGC CACCACAGCA GCCGACTCAG 
AAGCAGACAC AGCATCTTCA ATCAGGACAA GGCAATTTAT ACATCACGCC AAGCAATTCT 
GGTCTGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024451340.1
CDS	401..6472
Translation	MHGCGSGYALLVNAEVDSMGGVVDGGVGIATKTSPRRAAIEKAQVELRQEYDVREERRRELEFLEKGGNPLDFKFVNATSVSVQSTSLTDHHVEQFVTSEAKGSFPLTASPHGDSVESSGRPGATPVCEPNSADNFDGENELLEVERKRKHPSRRNNVTQSEQSSQMDGIHNAKESEDSAIFRPYARRNRSRPNRDGARSSSTDIVQSSVGHGSSLPARGDARDVKGLVTETDDHKAQIITSISNPKSTTSNGDLFFQIDTSNTQSNTELDCVQALKTVVNLPDDRLDVTESIVLRDNQHDQPSEADAEKAPNDIASRECDHGGGKELVISAGPECPPCAESAKTENETGPALLNGLEKDGNEGQNGNAAMGTERFNSESSCTQNSLSLDANNGCDPCDNRRNDDTNEILLKESSEFEGTRSLPSGNIGNEKKETNSISAINDGSVHENYSGNDSTVKNEEERRTTFHSQVKCTNLEGVEQNDHVASEADTKAGNMLADSSNSIREIIYPSGPQGSLDPPVQELPQPILLEKNSFVATDPQSCSNTHVKVVDKSHEDSILEEARVIEAKRKRIAELSVASVHSENRRRSHWDFVLEEMAWLANDVAQERLWKMTAAAQICRRIAFTSRLRVEEQNHHLKLKNVAYSLAKAVMQFWHSAKVYLSNNCHSVGSKNGKHEVGMFVGNEFSVNKFGDIDKEQVACKELEKQNPAKNIAHSIHGYAVRFLKYNSSPFPSFQAEAPATPDRIADLGIVDTSWDDRLTEESLFYAVPSGAMAMYRLSIESHIAQSEKTRSSMQEEVDTSMYDTPADFGYHDTAAYDEEEGETSAYYMHGVFEGSKSAKHDQKKRKSLTKSPSARSYDLGTDSPYGHCTTGPQQNVLMGKRPASNLNAGSIPTKRMRTASRQRFTSPFTAGTAGVLLQAPVKTDASSGDTNSFQDDQSILHGGSQIQKSVEVESAAHFERQLPYDYAETSTKPKKKKKAKHLGSAYEQGWQLDSTGHNEQRDNFKKRSESHHLDSNGTSGLYGQHTTKKPKISKQLLDNTFDNMVQMTGSIPSPAASQMSNMSNTNRFIKLIGGRERGRKNKSMKMSVGQPGSGSPWSLFEDQALVVLVHDMGPNWELISDAINSTAQFKCIFRKPKECKDRHKILMDKGAGDGADSAEDSGSSQSYPSTLPGIPKQGSARQLFQHLQGPMQEDTLKSHFEKIIIIGKKHHYKRSQNENQDPKQIAATHNSHFIALSQVCPNNLNGGVLTPLDLCDSSTSNPDVLPNVYQGSHASNLVMPNQGAVASTLPTSGAISSLQGSSGVVLGNNSSSPSGPLNAPHRDGRYNVPRTSLPVDEHQRMQPYQMLPSRNLQQSNMSVSGAVSGADRGVRMLSSGNGMGMMPGMNRSMPLPRSGFQGTASSSMLNSGSMLSNNVVGMSSPVNMHTGSGQGNLMRPREALHMLRLGHNHEHQRQMMVPELQMQPTQGNNQGISAFNGVPTAFANQTTTSPVQTYPGHPQQQHQMPAQQSNMLSNPHHPNLRGPNQATGSQHPAYAMHLPKGRQMQQRLLQQQQQQQQHFSASSALMPHVQHQSQLPISSSMQNSSQISPPSASQPVSLPAITPPSPMTPISMQQQQQQKHNLPHHAVSRSPQSGSSGLTNQMGKQRQRQPQQFQQSGRHHPQQRQHSQSPQQAKLLKGMGRGNMVVHQNLPNDHSPLNGLSVPPGNHGVEKGEQIMHLMQGQGLYSGTGLSPIHTSKPLGPSQSPNHSQPQQKLYSGPTTPSSKPLQQMPSHLESSTQGQVQPVPSGQTLTATHQNTPVMVPSHQHLQQHPQPHQKQVSQPQPTVQRMLQQSRLLNSDLPTKPQTDQGHADQQTSNNISQTGTSTSTGMPLACNDTSNVAPVVSSVSEMQWKSSEPSLHDSGMADTASKVGPIGSPPLTSAAGSEQVVPVSQGSVHRQLSGGLPLHCHNGGTRWQHQQPHQSTASPSPCQQLLQSQEPQLQQERQQSPQQLPPQQPTQKQTQHLQSGQGNLYITPSNSGLE

Target ORF information:

RefSeq Version	XM_024595572.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa chromatin modification-related protein EAF1 B (LOC7459484), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024595572.1

ORF Insert Sequence:

ATGCATGGAT GTGGTTCAGG ATATGCTCTC CTAGTAAATG CTGAGGTTGA TTCCATGGGT 
GGGGTTGTTG ATGGTGGAGT TGGTATTGCT ACCAAAACCT CTCCGCGGCG AGCAGCAATT 
GAGAAGGCCC AGGTGGAACT TAGACAAGAG TATGATGTTC GTGAGGAACG GAGAAGGGAG 
CTAGAATTTC TTGAGAAAGG CGGCAATCCT TTGGATTTTA AATTTGTAAA TGCAACTTCG 
GTCAGTGTCC AGTCTACTTC ACTCACAGAT CATCACGTTG AACAGTTTGT TACTAGTGAA 
GCAAAAGGCA GTTTTCCACT GACTGCATCA CCTCATGGGG ATTCTGTAGA AAGTAGTGGC 
AGACCAGGGG CTACTCCAGT TTGTGAGCCC AATAGTGCTG ACAATTTTGA TGGTGAAAAT 
GAGTTACTTG AAGTTGAAAG GAAACGTAAA CATCCTAGTA GGAGGAATAA TGTCACTCAA 
TCAGAGCAGT CTTCACAGAT GGATGGAATT CACAATGCCA AGGAATCAGA AGATTCTGCT 
ATTTTTCGGC CATATGCTCG AAGGAATAGG TCCAGACCAA ATCGTGACGG CGCGCGATCA 
AGTTCAACTG ATATTGTTCA GAGTTCAGTT GGCCATGGAT CCTCTTTACC GGCTCGTGGG 
GATGCAAGGG ATGTGAAAGG GTTGGTAACT GAAACAGACG ATCATAAAGC CCAGATTATT 
ACTTCCATCT CTAATCCAAA GTCCACCACT TCAAACGGTG ACCTGTTTTT TCAGATTGAC 
ACTTCCAATA CACAGTCAAA CACGGAGCTT GATTGTGTGC AGGCTCTGAA AACAGTAGTC 
AACCTGCCTG ATGATAGGTT GGATGTTACA GAGTCCATTG TCTTGAGGGA TAACCAACAT 
GACCAGCCTT CAGAAGCTGA TGCTGAGAAA GCACCCAATG ATATTGCTTC CAGGGAATGC 
GATCATGGTG GAGGAAAAGA GCTGGTTATT TCAGCTGGTC CTGAATGTCC CCCTTGTGCT 
GAATCAGCCA AGACTGAGAA TGAAACTGGT CCTGCTCTGC TCAATGGCTT GGAAAAAGAT 
GGAAATGAAG GTCAAAATGG TAATGCAGCA ATGGGAACGG AACGATTCAA TTCAGAGTCC 
TCTTGCACCC AAAATAGCTT AAGCCTGGAT GCAAACAATG GCTGTGATCC TTGTGATAAT 
CGTAGAAATG ATGACACCAA TGAAATTCTT TTGAAAGAAT CATCAGAATT TGAAGGAACA 
CGAAGTTTAC CATCGGGTAA TATAGGAAAT GAAAAGAAAG AGACTAACTC GATCTCTGCA 
ATCAATGATG GTTCTGTGCA TGAAAACTAC TCTGGCAATG ATTCTACTGT CAAAAATGAG 
GAAGAAAGGA GAACAACTTT TCACTCTCAG GTGAAGTGTA CTAATCTTGA AGGAGTGGAA 
CAAAATGACC ATGTTGCCTC TGAGGCTGAT ACAAAGGCGG GCAACATGTT GGCTGATAGT 
TCTAATTCCA TCAGGGAAAT AATTTACCCC AGTGGTCCCC AGGGCTCTTT GGATCCTCCT 
GTTCAAGAGC TTCCCCAGCC TATTTTGTTA GAGAAAAACT CTTTTGTTGC CACTGATCCT 
CAATCTTGTT CGAATACACA TGTTAAAGTG GTAGACAAGT CTCATGAAGA TTCTATTTTG 
GAAGAGGCAC GGGTTATAGA GGCTAAGCGC AAGAGGATTG CAGAATTATC TGTTGCATCT 
GTGCATTCAG AGAATCGCCG GAGATCTCAC TGGGATTTTG TCCTTGAAGA AATGGCATGG 
CTGGCAAATG ATGTTGCACA GGAGCGTCTC TGGAAGATGA CTGCTGCTGC TCAAATATGT 
CGACGAATTG CTTTTACTTC TCGATTGAGA GTTGAAGAGC AGAATCATCA TTTGAAGCTA 
AAAAACGTCG CTTACAGTCT TGCTAAGGCT GTCATGCAGT TCTGGCATTC AGCAAAGGTG 
TATCTAAGTA ACAATTGTCA CAGTGTTGGT TCAAAGAATG GGAAGCATGA GGTGGGGATG 
TTTGTTGGGA ATGAATTTTC TGTCAACAAG TTTGGAGATA TTGATAAGGA GCAGGTGGCA 
TGCAAAGAGT TAGAGAAACA AAATCCTGCC AAAAACATTG CGCATTCCAT TCATGGATAT 
GCTGTAAGGT TTTTGAAATA TAACAGTTCT CCATTTCCTT CCTTTCAAGC AGAAGCACCA 
GCAACTCCTG ATAGAATAGC TGATTTGGGC ATTGTGGACA CTTCATGGGA TGATCGCCTT 
ACTGAAGAAA GCCTGTTCTA CGCAGTTCCC TCAGGTGCCA TGGCAATGTA CAGATTGTCC 
ATTGAATCTC ATATTGCGCA GAGTGAGAAA ACTCGCAGTA GCATGCAGGA GGAAGTCGAT 
ACATCCATGT ATGACACTCC AGCAGATTTT GGATATCATG ATACTGCTGC ATACGATGAG 
GAGGAAGGAG AAACAAGTGC TTACTATATG CATGGAGTCT TTGAAGGTAG CAAGTCAGCA 
AAACATGACC AAAAGAAACG GAAAAGCTTA ACAAAGTCAC CCTCTGCAAG ATCTTATGAT 
CTGGGAACTG ATTCACCTTA TGGACACTGC ACCACTGGGC CACAACAAAA TGTATTGATG 
GGGAAAAGAC CTGCTAGTAA TCTTAATGCT GGCTCAATCC CAACAAAACG CATGCGTACT 
GCTTCTAGAC AGAGGTTTAC AAGTCCTTTT ACTGCTGGAA CTGCTGGGGT TTTACTCCAG 
GCTCCAGTGA AGACAGATGC ATCGAGTGGA GATACCAATT CTTTTCAGGA TGATCAGAGT 
ATTTTGCATG GTGGGTCACA AATTCAGAAA AGTGTGGAGG TTGAGTCCGC TGCGCATTTC 
GAAAGGCAGT TACCATATGA CTACGCAGAG ACATCAACAA AACCGAAGAA GAAGAAGAAG 
GCAAAGCACC TGGGTTCTGC TTATGAGCAG GGGTGGCAGT TGGATTCTAC TGGTCATAAT 
GAACAGAGGG ATAATTTCAA AAAGAGATCG GAGAGTCATC ATTTGGACTC TAATGGAACC 
AGTGGTTTAT ATGGGCAACA TACCACAAAG AAGCCAAAGA TATCTAAGCA ATTGCTAGAT 
AATACTTTTG ACAACATGGT TCAAATGACC GGGTCCATCC CATCTCCAGC TGCTTCCCAG 
ATGAGTAATA TGTCAAACAC AAATAGATTC ATCAAATTGA TTGGTGGCAG GGAACGAGGC 
AGAAAAAATA AATCAATGAA GATGTCTGTT GGGCAGCCAG GTTCTGGAAG TCCCTGGTCA 
CTTTTTGAAG ACCAGGCTCT TGTTGTTCTT GTGCATGACA TGGGTCCTAA TTGGGAGCTT 
ATAAGCGATG CTATCAACAG CACTGCTCAA TTCAAGTGTA TATTCCGCAA GCCTAAAGAA 
TGCAAAGATC GTCACAAAAT CTTAATGGAT AAGGGTGCTG GTGATGGGGC TGATAGTGCT 
GAGGATTCAG GATCTTCTCA ATCCTACCCA TCAACTTTGC CTGGCATTCC AAAGCAGGGC 
AGTGCCAGAC AGTTGTTTCA ACATTTGCAA GGGCCGATGC AAGAGGATAC CCTGAAGTCT 
CATTTTGAGA AAATCATCAT TATTGGGAAG AAACATCATT ACAAAAGGAG TCAGAATGAA 
AACCAGGATC CCAAGCAAAT AGCAGCAACT CACAATTCTC ATTTTATTGC TCTTTCCCAA 
GTTTGCCCAA ACAACTTAAA TGGAGGTGTC CTAACGCCCC TTGATCTCTG TGATTCAAGC 
ACTTCAAATC CAGATGTTCT TCCCAATGTG TATCAAGGTT CTCATGCTAG CAATTTGGTA 
ATGCCAAACC AAGGTGCTGT TGCATCAACG CTTCCCACTT CTGGTGCAAT CTCTTCTCTG 
CAAGGATCTT CTGGCGTGGT TCTTGGCAAT AACTCATCAT CACCATCTGG TCCCCTCAAT 
GCACCTCATA GAGATGGTAG ATACAATGTT CCAAGGACAT CTTTACCAGT TGACGAGCAT 
CAAAGAATGC AACCCTATCA AATGTTACCT AGTAGAAACT TGCAGCAGTC TAACATGTCG 
GTTTCTGGGG CTGTCTCTGG AGCTGATCGT GGTGTTCGCA TGTTATCGAG TGGAAATGGT 
ATGGGCATGA TGCCAGGGAT GAACAGAAGC ATGCCACTGC CACGGTCAGG ATTTCAAGGA 
ACAGCCTCTT CATCAATGCT GAATTCTGGA AGTATGCTTT CTAACAATGT GGTTGGGATG 
TCAAGCCCTG TGAATATGCA CACTGGTTCT GGTCAAGGGA ACTTGATGAG ACCTCGCGAG 
GCTTTGCATA TGTTGAGGCT TGGCCATAAT CATGAGCATC AAAGACAAAT GATGGTACCA 
GAGCTTCAGA TGCAGCCAAC ACAAGGGAAC AACCAAGGAA TTTCTGCTTT CAACGGGGTG 
CCTACTGCTT TTGCTAATCA GACAACTACT TCACCTGTGC AGACATATCC AGGCCATCCC 
CAGCAGCAGC ATCAAATGCC TGCACAACAG TCCAATATGC TTAGCAATCC TCATCATCCT 
AATCTCCGTG GCCCCAATCA GGCTACAGGT TCACAGCATC CAGCATATGC TATGCACCTG 
CCTAAAGGAA GGCAAATGCA GCAGCGGCTT CTCCAGCAGC AGCAGCAGCA GCAGCAGCAC 
TTTTCTGCAT CTAGTGCTTT GATGCCCCAT GTCCAACATC AATCCCAACT TCCCATATCA 
TCGTCTATGC AAAATAGTTC TCAAATCTCA CCACCATCTG CATCGCAGCC GGTGTCACTA 
CCTGCTATAA CTCCCCCTTC CCCAATGACT CCCATTTCAA TGCAGCAGCA ACAGCAGCAG 
AAACACAACT TGCCACATCA TGCGGTCAGT CGGAGCCCCC AAAGTGGTTC CAGTGGGTTG 
ACCAATCAGA TGGGAAAGCA GCGACAACGG CAACCACAGC AGTTTCAACA ATCTGGCAGA 
CATCACCCTC AACAACGACA ACACTCACAG TCTCCACAGC AGGCTAAACT TTTGAAGGGA 
ATGGGGAGAG GGAACATGGT GGTGCACCAG AACCTTCCCA ATGATCACTC TCCTTTGAAT 
GGCCTTTCTG TGCCTCCAGG AAACCATGGT GTAGAGAAAG GAGAGCAAAT CATGCACTTG 
ATGCAGGGTC AAGGCTTGTA TTCTGGGACT GGGTTGAGTC CAATTCATAC ATCTAAACCA 
TTGGGTCCTT CACAATCTCC AAACCATTCT CAGCCACAGC AAAAGCTATA TTCGGGCCCT 
ACAACCCCTT CATCAAAGCC ACTCCAGCAA ATGCCTTCTC ATTTGGAAAG TAGTACTCAA 
GGCCAGGTCC AACCAGTACC CTCTGGTCAA ACACTAACAG CTACTCACCA GAACACTCCA 
GTTATGGTTC CCAGTCATCA GCATCTTCAA CAACATCCAC AGCCACATCA GAAGCAGGTT 
AGTCAACCTC AACCAACTGT TCAGAGAATG CTTCAACAGA GTCGTCTGTT GAATTCTGAT 
CTGCCAACAA AGCCTCAAAC TGATCAGGGC CATGCTGATC AGCAAACTTC AAACAACATC 
TCCCAGACAG GAACAAGTAC ATCCACAGGG ATGCCTTTGG CCTGTAATGA TACATCTAAT 
GTGGCACCAG TTGTTTCTTC TGTTTCTGAG ATGCAGTGGA AATCATCAGA ACCATCCTTG 
CATGATTCTG GCATGGCAGA TACAGCTTCT AAGGTAGGTC CTATTGGTAG CCCCCCCCTT 
ACGAGTGCAG CTGGGAGTGA GCAAGTGGTG CCTGTCAGCC AAGGTTCAGT CCACAGGCAG 
TTATCAGGAG GCTTGCCCCT GCATTGCCAT AATGGTGGGA CACGGTGGCA GCACCAACAA 
CCACATCAAT CAACAGCATC GCCTTCCCCA TGCCAGCAAC TTTTGCAGTC ACAAGAGCCA 
CAACTACAGC AAGAACGACA ACAATCACCT CAACAGCTGC CACCACAGCA GCCGACTCAG 
AAGCAGACAC AGCATCTTCA ATCAGGACAA GGCAATTTAT ACATCACGCC AAGCAATTCT 
GGTCTGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7459484 ( XM_024595575.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024451343.1 Populus trichocarpa chromatin modification-related protein EAF1 B (LOC7459484), transcript variant X5, mRNA.

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CloneID	OPa424909
Clone ID Related Accession (Same CDS sequence)	XM_024595575.1
Accession Version	XM_024595575.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	chromatin modification-related protein EAF1 B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037286.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGGAT GTGGTTCAGG ATATGCTCTC CTAGTAAATG CTGAGGTTGA TTCCATGGGT 
GGGGTTGTTG ATGGTGGAGT TGGTATTGCT ACCAAAACCT CTCCGCGGCG AGCAGCAATT 
GAGAAGGCCC AGGTGGAACT TAGACAAGAG TATGATGTTC GTGAGGAACG GAGAAGGGAG 
CTAGAATTTC TTGAGAAAGG CGGCAATCCT TTGGATTTTA AATTTGTAAA TGCAACTTCG 
GTCAGTGTCC AGTCTACTTC ACTCACAGAT CATCACGTTG AACAGTTTGT TACTAGTGAA 
GCAAAAGGCA GTTTTCCACT GACTGCATCA CCTCATGGGG ATTCTGTAGA AAGTAGTGGC 
AGACCAGGGG CTACTCCAGT TTGTGAGCCC AATAGTGCTG ACAATTTTGA TGGTGAAAAT 
GAGTTACTTG AAGTTGAAAG GAAACGTAAA CATCCTAGTA GGAGGAATAA TGTCACTCAA 
TCAGAGCAGT CTTCACAGAT GGATGGAATT CACAATGCCA AGGAATCAGA AGATTCTGCT 
ATTTTTCGGC CATATGCTCG AAGGAATAGG TCCAGACCAA ATCGTGACGG CGCGCGATCA 
AGTTCAACTG ATATTGTTCA GAGTTCAGTT GGCCATGGAT CCTCTTTACC GGCTCGTGGG 
GATGCAAGGG ATGTGAAAGG GTTGGTAACT GAAACAGACG ATCATAAAGC CCAGATTATT 
ACTTCCATCT CTAATCCAAA GTCCACCACT TCAAACGGTG ACCTGTTTTT TCAGATTGAC 
ACTTCCAATA CACAGTCAAA CACGGAGCTT GATTGTGTGC AGGCTCTGAA AACAGTAGTC 
AACCTGCCTG ATGATAGGTT GGATGTTACA GAGTCCATTG TCTTGAGGGA TAACCAACAT 
GACCAGCCTT CAGAAGCTGA TGCTGAGAAA GCACCCAATG ATATTGCTTC CAGGGAATGC 
GATCATGGTG GAGGAAAAGA GCTGGTTATT TCAGCTGGTC CTGAATGTCC CCCTTGTGCT 
GAATCAGCCA AGACTGAGAA TGAAACTGGT CCTGCTCTGC TCAATGGCTT GGAAAAAGAT 
GGAAATGAAG GTCAAAATGG TAATGCAGCA ATGGGAACGG AACGATTCAA TTCAGAGTCC 
TCTTGCACCC AAAATAGCTT AAGCCTGGAT GCAAACAATG GCTGTGATCC TTGTGATAAT 
CGTAGAAATG ATGACACCAA TGAAATTCTT TTGAAAGAAT CATCAGAATT TGAAGGAACA 
CGAAGTTTAC CATCGGGTAA TATAGGAAAT GAAAAGAAAG AGACTAACTC GATCTCTGCA 
ATCAATGATG GTTCTGTGCA TGAAAACTAC TCTGGCAATG ATTCTACTGT CAAAAATGAG 
GAAGAAAGGA GAACAACTTT TCACTCTCAG GTGAAGTGTA CTAATCTTGA AGGAGTGGAA 
CAAAATGACC ATGTTGCCTC TGAGGCTGAT ACAAAGGCGG GCAACATGTT GGCTGATAGT 
TCTAATTCCA TCAGGGAAAT AATTTACCCC AGTGGTCCCC AGGGCTCTTT GGATCCTCCT 
GTTCAAGAGC TTCCCCAGCC TATTTTGTTA GAGAAAAACT CTTTTGTTGC CACTGATCCT 
CAATCTTGTT CGAATACACA TGTTAAAGTG GTAGACAAGT CTCATGAAGA TTCTATTTTG 
GAAGAGGCAC GGGTTATAGA GGCTAAGCGC AAGAGGATTG CAGAATTATC TGTTGCATCT 
GTGCATTCAG AGAATCGCCG GAGATCTCAC TGGGATTTTG TCCTTGAAGA AATGGCATGG 
CTGGCAAATG ATGTTGCACA GGAGCGTCTC TGGAAGATGA CTGCTGCTGC TCAAATATGT 
CGACGAATTG CTTTTACTTC TCGATTGAGA GTTGAAGAGC AGAATCATCA TTTGAAGCTA 
AAAAACGTCG CTTACAGTCT TGCTAAGGCT GTCATGCAGT TCTGGCATTC AGCAAAGGTG 
TATCTAAGTA ACAATTGTCA CAGTGTTGGT TCAAAGAATG GGAAGCATGA GGTGGGGATG 
TTTGTTGGGA ATGAATTTTC TGTCAACAAG TTTGGAGATA TTGATAAGGA GCAGGTGGCA 
TGCAAAGAGT TAGAGAAACA AAATCCTGCC AAAAACATTG CGCATTCCAT TCATGGATAT 
GCTGTAAGGT TTTTGAAATA TAACAGTTCT CCATTTCCTT CCTTTCAAGC AGAAGCACCA 
GCAACTCCTG ATAGAATAGC TGATTTGGGC ATTGTGGACA CTTCATGGGA TGATCGCCTT 
ACTGAAGAAA GCCTGTTCTA CGCAGTTCCC TCAGGTGCCA TGGCAATGTA CAGATTGTCC 
ATTGAATCTC ATATTGCGCA GAGTGAGAAA ACTCGCAGTA GCATGCAGGA GGAAGTCGAT 
ACATCCATGT ATGACACTCC AGCAGATTTT GGATATCATG ATACTGCTGC ATACGATGAG 
GAGGAAGGAG AAACAAGTGC TTACTATATG CATGGAGTCT TTGAAGGTAG CAAGTCAGCA 
AAACATGACC AAAAGAAACG GAAAAGCTTA ACAAAGTCAC CCTCTGCAAG ATCTTATGAT 
CTGGGAACTG ATTCACCTTA TGGACACTGC ACCACTGGGC CACAACAAAA TGTATTGATG 
GGGAAAAGAC CTGCTAGTAA TCTTAATGCT GGCTCAATCC CAACAAAACG CATGCGTACT 
GCTTCTAGAC AGAGGTTTAC AAGTCCTTTT ACTGCTGGAA CTGCTGGGGT TTTACTCCAG 
GCTCCAGTGA AGACAGATGC ATCGAGTGGA GATACCAATT CTTTTCAGGA TGATCAGAGT 
ATTTTGCATG GTGGGTCACA AATTCAGAAA AGTGTGGAGG TTGAGTCCGC TGCGCATTTC 
GAAAGGCAGT TACCATATGA CTACGCAGAG ACATCAACAA AACCGAAGAA GAAGAAGAAG 
GCAAAGCACC TGGGTTCTGC TTATGAGCAG GGGTGGCAGT TGGATTCTAC TGGTCATAAT 
GAACAGAGGG ATAATTTCAA AAAGAGATCG GAGAGTCATC ATTTGGACTC TAATGGAACC 
AGTGGTTTAT ATGGGCAACA TACCACAAAG AAGCCAAAGA TATCTAAGCA ATTGCTAGAT 
AATACTTTTG ACAACATGGT TCAAATGACC GGGTCCATCC CATCTCCAGC TGCTTCCCAG 
ATGAGTAATA TGTCAAACAC AAATAGATTC ATCAAATTGA TTGGTGGCAG GGAACGAGGC 
AGAAAAAATA AATCAATGAA GATGTCTGTT GGGCAGCCAG GTTCTGGAAG TCCCTGGTCA 
CTTTTTGAAG ACCAGGCTCT TGTTGTTCTT GTGCATGACA TGGGTCCTAA TTGGGAGCTT 
ATAAGCGATG CTATCAACAG CACTGCTCAA TTCAAGTGTA TATTCCGCAA GCCTAAAGAA 
TGCAAAGATC GTCACAAAAT CTTAATGGAT AAGGGTGCTG GTGATGGGGC TGATAGTGCT 
GAGGATTCAG GATCTTCTCA ATCCTACCCA TCAACTTTGC CTGGCATTCC AAAGGGCAGT 
GCCAGACAGT TGTTTCAACA TTTGCAAGGG CCGATGCAAG AGGATACCCT GAAGTCTCAT 
TTTGAGAAAA TCATCATTAT TGGGAAGAAA CATCATTACA AAAGGAGTCA GAATGAAAAC 
CAGGATCCCA AGCAAATAGC AGCAACTCAC AATTCTCATT TTATTGCTCT TTCCCAAGTT 
TGCCCAAACA ACTTAAATGG AGGTGTCCTA ACGCCCCTTG ATCTCTGTGA TTCAAGCACT 
TCAAATCCAG ATGTTCTTCC CAATGTGTAT CAAGGTTCTC ATGCTAGCAA TTTGGTAATG 
CCAAACCAAG GTGCTGTTGC ATCAACGCTT CCCACTTCTG GTGCAATCTC TTCTCTGCAA 
GGATCTTCTG GCGTGGTTCT TGGCAATAAC TCATCATCAC CATCTGGTCC CCTCAATGCA 
CCTCATAGAG ATGGTAGATA CAATGTTCCA AGGACATCTT TACCAGTTGA CGAGCATCAA 
AGAATGCAAC CCTATCAAAT GTTACCTAGT AGAAACTTGC AGCAGTCTAA CATGTCGGTT 
TCTGGGGCTG TCTCTGGAGC TGATCGTGGT GTTCGCATGT TATCGAGTGG AAATGGTATG 
GGCATGATGC CAGGGATGAA CAGAAGCATG CCACTGCCAC GGTCAGGATT TCAAGGAACA 
GCCTCTTCAT CAATGCTGAA TTCTGGAAGT ATGCTTTCTA ACAATGTGGT TGGGATGTCA 
AGCCCTGTGA ATATGCACAC TGGTTCTGGT CAAGGGAACT TGATGAGACC TCGCGAGGCT 
TTGCATATGT TGAGGCTTGG CCATAATCAT GAGCATCAAA GACAAATGAT GGTACCAGAG 
CTTCAGATGC AGCCAACACA AGGGAACAAC CAAGGAATTT CTGCTTTCAA CGGGGTGCCT 
ACTGCTTTTG CTAATCAGAC AACTACTTCA CCTGTGCAGA CATATCCAGG CCATCCCCAG 
CAGCAGCATC AAATGCCTGC ACAACAGTCC AATATGCTTA GCAATCCTCA TCATCCTAAT 
CTCCGTGGCC CCAATCAGGC TACAGGTTCA CAGCATCCAG CATATGCTAT GCACCTGCCT 
AAAGGAAGGC AAATGCAGCA GCGGCTTCTC CAGCAGCAGC AGCAGCAGCA GCAGCACTTT 
TCTGCATCTA GTGCTTTGAT GCCCCATGTC CAACATCAAT CCCAACTTCC CATATCATCG 
TCTATGCAAA ATAGTTCTCA AATCTCACCA CCATCTGCAT CGCAGCCGGT GTCACTACCT 
GCTATAACTC CCCCTTCCCC AATGACTCCC ATTTCAATGC AGCAGCAACA GCAGCAGAAA 
CACAACTTGC CACATCATGC GGTCAGTCGG AGCCCCCAAA GTGGTTCCAG TGGGTTGACC 
AATCAGATGG GAAAGCAGCG ACAACGGCAA CCACAGCAGT TTCAACAATC TGGCAGACAT 
CACCCTCAAC AACGACAACA CTCACAGTCT CCACAGCAGG CTAAACTTTT GAAGGGAATG 
GGGAGAGGGA ACATGGTGGT GCACCAGAAC CTTCCCAATG ATCACTCTCC TTTGAATGGC 
CTTTCTGTGC CTCCAGGAAA CCATGGTGTA GAGAAAGGAG AGCAAATCAT GCACTTGATG 
CAGGGTCAAG GCTTGTATTC TGGGACTGGG TTGAGTCCAA TTCATACATC TAAACCATTG 
GGTCCTTCAC AATCTCCAAA CCATTCTCAG CCACAGCAAA AGCTATATTC GGGCCCTACA 
ACCCCTTCAT CAAAGCCACT CCAGCAAATG CCTTCTCATT TGGAAAGTAG TACTCAAGGC 
CAGGTCCAAC CAGTACCCTC TGGTCAAACA CTAACAGCTA CTCACCAGAA CACTCCAGTT 
ATGGTTCCCA GTCATCAGCA TCTTCAACAA CATCCACAGC CACATCAGAA GCAGGTTAGT 
CAACCTCAAC CAACTGTTCA GAGAATGCTT CAACAGAGTC GTCTGTTGAA TTCTGATCTG 
CCAACAAAGC CTCAAACTGA TCAGGGCCAT GCTGATCAGC AAACTTCAAA CAACATCTCC 
CAGACAGGAA CAAGTACATC CACAGGGATG CCTTTGGCCT GTAATGATAC ATCTAATGTG 
GCACCAGTTG TTTCTTCTGT TTCTGAGATG CAGTGGAAAT CATCAGAACC ATCCTTGCAT 
GATTCTGGCA TGGCAGATAC AGCTTCTAAG GTAGGTCCTA TTGGTAGCCC CCCCCTTACG 
AGTGCAGCTG GGAGTGAGCA AGTGGTGCCT GTCAGCCAAG GTTCAGTCCA CAGGCAGTTA 
TCAGGAGGCT TGCCCCTGCA TTGCCATAAT GGTGGGACAC GGTGGCAGCA CCAACAACCA 
CATCAATCAA CAGCATCGCC TTCCCCATGC CAGCAACTTT TGCAGTCACA AGAGCCACAA 
CTACAGCAAG AACGACAACA ATCACCTCAA CAGCTGCCAC CACAGCAGCC GACTCAGAAG 
CAGACACAGC ATCTTCAATC AGGACAAGGC AATTTATACA TCACGCCAAG CAATTCTGGT 
CTGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024451343.1
CDS	401..6469
Translation	MHGCGSGYALLVNAEVDSMGGVVDGGVGIATKTSPRRAAIEKAQVELRQEYDVREERRRELEFLEKGGNPLDFKFVNATSVSVQSTSLTDHHVEQFVTSEAKGSFPLTASPHGDSVESSGRPGATPVCEPNSADNFDGENELLEVERKRKHPSRRNNVTQSEQSSQMDGIHNAKESEDSAIFRPYARRNRSRPNRDGARSSSTDIVQSSVGHGSSLPARGDARDVKGLVTETDDHKAQIITSISNPKSTTSNGDLFFQIDTSNTQSNTELDCVQALKTVVNLPDDRLDVTESIVLRDNQHDQPSEADAEKAPNDIASRECDHGGGKELVISAGPECPPCAESAKTENETGPALLNGLEKDGNEGQNGNAAMGTERFNSESSCTQNSLSLDANNGCDPCDNRRNDDTNEILLKESSEFEGTRSLPSGNIGNEKKETNSISAINDGSVHENYSGNDSTVKNEEERRTTFHSQVKCTNLEGVEQNDHVASEADTKAGNMLADSSNSIREIIYPSGPQGSLDPPVQELPQPILLEKNSFVATDPQSCSNTHVKVVDKSHEDSILEEARVIEAKRKRIAELSVASVHSENRRRSHWDFVLEEMAWLANDVAQERLWKMTAAAQICRRIAFTSRLRVEEQNHHLKLKNVAYSLAKAVMQFWHSAKVYLSNNCHSVGSKNGKHEVGMFVGNEFSVNKFGDIDKEQVACKELEKQNPAKNIAHSIHGYAVRFLKYNSSPFPSFQAEAPATPDRIADLGIVDTSWDDRLTEESLFYAVPSGAMAMYRLSIESHIAQSEKTRSSMQEEVDTSMYDTPADFGYHDTAAYDEEEGETSAYYMHGVFEGSKSAKHDQKKRKSLTKSPSARSYDLGTDSPYGHCTTGPQQNVLMGKRPASNLNAGSIPTKRMRTASRQRFTSPFTAGTAGVLLQAPVKTDASSGDTNSFQDDQSILHGGSQIQKSVEVESAAHFERQLPYDYAETSTKPKKKKKAKHLGSAYEQGWQLDSTGHNEQRDNFKKRSESHHLDSNGTSGLYGQHTTKKPKISKQLLDNTFDNMVQMTGSIPSPAASQMSNMSNTNRFIKLIGGRERGRKNKSMKMSVGQPGSGSPWSLFEDQALVVLVHDMGPNWELISDAINSTAQFKCIFRKPKECKDRHKILMDKGAGDGADSAEDSGSSQSYPSTLPGIPKGSARQLFQHLQGPMQEDTLKSHFEKIIIIGKKHHYKRSQNENQDPKQIAATHNSHFIALSQVCPNNLNGGVLTPLDLCDSSTSNPDVLPNVYQGSHASNLVMPNQGAVASTLPTSGAISSLQGSSGVVLGNNSSSPSGPLNAPHRDGRYNVPRTSLPVDEHQRMQPYQMLPSRNLQQSNMSVSGAVSGADRGVRMLSSGNGMGMMPGMNRSMPLPRSGFQGTASSSMLNSGSMLSNNVVGMSSPVNMHTGSGQGNLMRPREALHMLRLGHNHEHQRQMMVPELQMQPTQGNNQGISAFNGVPTAFANQTTTSPVQTYPGHPQQQHQMPAQQSNMLSNPHHPNLRGPNQATGSQHPAYAMHLPKGRQMQQRLLQQQQQQQQHFSASSALMPHVQHQSQLPISSSMQNSSQISPPSASQPVSLPAITPPSPMTPISMQQQQQQKHNLPHHAVSRSPQSGSSGLTNQMGKQRQRQPQQFQQSGRHHPQQRQHSQSPQQAKLLKGMGRGNMVVHQNLPNDHSPLNGLSVPPGNHGVEKGEQIMHLMQGQGLYSGTGLSPIHTSKPLGPSQSPNHSQPQQKLYSGPTTPSSKPLQQMPSHLESSTQGQVQPVPSGQTLTATHQNTPVMVPSHQHLQQHPQPHQKQVSQPQPTVQRMLQQSRLLNSDLPTKPQTDQGHADQQTSNNISQTGTSTSTGMPLACNDTSNVAPVVSSVSEMQWKSSEPSLHDSGMADTASKVGPIGSPPLTSAAGSEQVVPVSQGSVHRQLSGGLPLHCHNGGTRWQHQQPHQSTASPSPCQQLLQSQEPQLQQERQQSPQQLPPQQPTQKQTQHLQSGQGNLYITPSNSGLE

Target ORF information:

RefSeq Version	XM_024595575.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa chromatin modification-related protein EAF1 B (LOC7459484), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024595575.1

ORF Insert Sequence:

ATGCATGGAT GTGGTTCAGG ATATGCTCTC CTAGTAAATG CTGAGGTTGA TTCCATGGGT 
GGGGTTGTTG ATGGTGGAGT TGGTATTGCT ACCAAAACCT CTCCGCGGCG AGCAGCAATT 
GAGAAGGCCC AGGTGGAACT TAGACAAGAG TATGATGTTC GTGAGGAACG GAGAAGGGAG 
CTAGAATTTC TTGAGAAAGG CGGCAATCCT TTGGATTTTA AATTTGTAAA TGCAACTTCG 
GTCAGTGTCC AGTCTACTTC ACTCACAGAT CATCACGTTG AACAGTTTGT TACTAGTGAA 
GCAAAAGGCA GTTTTCCACT GACTGCATCA CCTCATGGGG ATTCTGTAGA AAGTAGTGGC 
AGACCAGGGG CTACTCCAGT TTGTGAGCCC AATAGTGCTG ACAATTTTGA TGGTGAAAAT 
GAGTTACTTG AAGTTGAAAG GAAACGTAAA CATCCTAGTA GGAGGAATAA TGTCACTCAA 
TCAGAGCAGT CTTCACAGAT GGATGGAATT CACAATGCCA AGGAATCAGA AGATTCTGCT 
ATTTTTCGGC CATATGCTCG AAGGAATAGG TCCAGACCAA ATCGTGACGG CGCGCGATCA 
AGTTCAACTG ATATTGTTCA GAGTTCAGTT GGCCATGGAT CCTCTTTACC GGCTCGTGGG 
GATGCAAGGG ATGTGAAAGG GTTGGTAACT GAAACAGACG ATCATAAAGC CCAGATTATT 
ACTTCCATCT CTAATCCAAA GTCCACCACT TCAAACGGTG ACCTGTTTTT TCAGATTGAC 
ACTTCCAATA CACAGTCAAA CACGGAGCTT GATTGTGTGC AGGCTCTGAA AACAGTAGTC 
AACCTGCCTG ATGATAGGTT GGATGTTACA GAGTCCATTG TCTTGAGGGA TAACCAACAT 
GACCAGCCTT CAGAAGCTGA TGCTGAGAAA GCACCCAATG ATATTGCTTC CAGGGAATGC 
GATCATGGTG GAGGAAAAGA GCTGGTTATT TCAGCTGGTC CTGAATGTCC CCCTTGTGCT 
GAATCAGCCA AGACTGAGAA TGAAACTGGT CCTGCTCTGC TCAATGGCTT GGAAAAAGAT 
GGAAATGAAG GTCAAAATGG TAATGCAGCA ATGGGAACGG AACGATTCAA TTCAGAGTCC 
TCTTGCACCC AAAATAGCTT AAGCCTGGAT GCAAACAATG GCTGTGATCC TTGTGATAAT 
CGTAGAAATG ATGACACCAA TGAAATTCTT TTGAAAGAAT CATCAGAATT TGAAGGAACA 
CGAAGTTTAC CATCGGGTAA TATAGGAAAT GAAAAGAAAG AGACTAACTC GATCTCTGCA 
ATCAATGATG GTTCTGTGCA TGAAAACTAC TCTGGCAATG ATTCTACTGT CAAAAATGAG 
GAAGAAAGGA GAACAACTTT TCACTCTCAG GTGAAGTGTA CTAATCTTGA AGGAGTGGAA 
CAAAATGACC ATGTTGCCTC TGAGGCTGAT ACAAAGGCGG GCAACATGTT GGCTGATAGT 
TCTAATTCCA TCAGGGAAAT AATTTACCCC AGTGGTCCCC AGGGCTCTTT GGATCCTCCT 
GTTCAAGAGC TTCCCCAGCC TATTTTGTTA GAGAAAAACT CTTTTGTTGC CACTGATCCT 
CAATCTTGTT CGAATACACA TGTTAAAGTG GTAGACAAGT CTCATGAAGA TTCTATTTTG 
GAAGAGGCAC GGGTTATAGA GGCTAAGCGC AAGAGGATTG CAGAATTATC TGTTGCATCT 
GTGCATTCAG AGAATCGCCG GAGATCTCAC TGGGATTTTG TCCTTGAAGA AATGGCATGG 
CTGGCAAATG ATGTTGCACA GGAGCGTCTC TGGAAGATGA CTGCTGCTGC TCAAATATGT 
CGACGAATTG CTTTTACTTC TCGATTGAGA GTTGAAGAGC AGAATCATCA TTTGAAGCTA 
AAAAACGTCG CTTACAGTCT TGCTAAGGCT GTCATGCAGT TCTGGCATTC AGCAAAGGTG 
TATCTAAGTA ACAATTGTCA CAGTGTTGGT TCAAAGAATG GGAAGCATGA GGTGGGGATG 
TTTGTTGGGA ATGAATTTTC TGTCAACAAG TTTGGAGATA TTGATAAGGA GCAGGTGGCA 
TGCAAAGAGT TAGAGAAACA AAATCCTGCC AAAAACATTG CGCATTCCAT TCATGGATAT 
GCTGTAAGGT TTTTGAAATA TAACAGTTCT CCATTTCCTT CCTTTCAAGC AGAAGCACCA 
GCAACTCCTG ATAGAATAGC TGATTTGGGC ATTGTGGACA CTTCATGGGA TGATCGCCTT 
ACTGAAGAAA GCCTGTTCTA CGCAGTTCCC TCAGGTGCCA TGGCAATGTA CAGATTGTCC 
ATTGAATCTC ATATTGCGCA GAGTGAGAAA ACTCGCAGTA GCATGCAGGA GGAAGTCGAT 
ACATCCATGT ATGACACTCC AGCAGATTTT GGATATCATG ATACTGCTGC ATACGATGAG 
GAGGAAGGAG AAACAAGTGC TTACTATATG CATGGAGTCT TTGAAGGTAG CAAGTCAGCA 
AAACATGACC AAAAGAAACG GAAAAGCTTA ACAAAGTCAC CCTCTGCAAG ATCTTATGAT 
CTGGGAACTG ATTCACCTTA TGGACACTGC ACCACTGGGC CACAACAAAA TGTATTGATG 
GGGAAAAGAC CTGCTAGTAA TCTTAATGCT GGCTCAATCC CAACAAAACG CATGCGTACT 
GCTTCTAGAC AGAGGTTTAC AAGTCCTTTT ACTGCTGGAA CTGCTGGGGT TTTACTCCAG 
GCTCCAGTGA AGACAGATGC ATCGAGTGGA GATACCAATT CTTTTCAGGA TGATCAGAGT 
ATTTTGCATG GTGGGTCACA AATTCAGAAA AGTGTGGAGG TTGAGTCCGC TGCGCATTTC 
GAAAGGCAGT TACCATATGA CTACGCAGAG ACATCAACAA AACCGAAGAA GAAGAAGAAG 
GCAAAGCACC TGGGTTCTGC TTATGAGCAG GGGTGGCAGT TGGATTCTAC TGGTCATAAT 
GAACAGAGGG ATAATTTCAA AAAGAGATCG GAGAGTCATC ATTTGGACTC TAATGGAACC 
AGTGGTTTAT ATGGGCAACA TACCACAAAG AAGCCAAAGA TATCTAAGCA ATTGCTAGAT 
AATACTTTTG ACAACATGGT TCAAATGACC GGGTCCATCC CATCTCCAGC TGCTTCCCAG 
ATGAGTAATA TGTCAAACAC AAATAGATTC ATCAAATTGA TTGGTGGCAG GGAACGAGGC 
AGAAAAAATA AATCAATGAA GATGTCTGTT GGGCAGCCAG GTTCTGGAAG TCCCTGGTCA 
CTTTTTGAAG ACCAGGCTCT TGTTGTTCTT GTGCATGACA TGGGTCCTAA TTGGGAGCTT 
ATAAGCGATG CTATCAACAG CACTGCTCAA TTCAAGTGTA TATTCCGCAA GCCTAAAGAA 
TGCAAAGATC GTCACAAAAT CTTAATGGAT AAGGGTGCTG GTGATGGGGC TGATAGTGCT 
GAGGATTCAG GATCTTCTCA ATCCTACCCA TCAACTTTGC CTGGCATTCC AAAGGGCAGT 
GCCAGACAGT TGTTTCAACA TTTGCAAGGG CCGATGCAAG AGGATACCCT GAAGTCTCAT 
TTTGAGAAAA TCATCATTAT TGGGAAGAAA CATCATTACA AAAGGAGTCA GAATGAAAAC 
CAGGATCCCA AGCAAATAGC AGCAACTCAC AATTCTCATT TTATTGCTCT TTCCCAAGTT 
TGCCCAAACA ACTTAAATGG AGGTGTCCTA ACGCCCCTTG ATCTCTGTGA TTCAAGCACT 
TCAAATCCAG ATGTTCTTCC CAATGTGTAT CAAGGTTCTC ATGCTAGCAA TTTGGTAATG 
CCAAACCAAG GTGCTGTTGC ATCAACGCTT CCCACTTCTG GTGCAATCTC TTCTCTGCAA 
GGATCTTCTG GCGTGGTTCT TGGCAATAAC TCATCATCAC CATCTGGTCC CCTCAATGCA 
CCTCATAGAG ATGGTAGATA CAATGTTCCA AGGACATCTT TACCAGTTGA CGAGCATCAA 
AGAATGCAAC CCTATCAAAT GTTACCTAGT AGAAACTTGC AGCAGTCTAA CATGTCGGTT 
TCTGGGGCTG TCTCTGGAGC TGATCGTGGT GTTCGCATGT TATCGAGTGG AAATGGTATG 
GGCATGATGC CAGGGATGAA CAGAAGCATG CCACTGCCAC GGTCAGGATT TCAAGGAACA 
GCCTCTTCAT CAATGCTGAA TTCTGGAAGT ATGCTTTCTA ACAATGTGGT TGGGATGTCA 
AGCCCTGTGA ATATGCACAC TGGTTCTGGT CAAGGGAACT TGATGAGACC TCGCGAGGCT 
TTGCATATGT TGAGGCTTGG CCATAATCAT GAGCATCAAA GACAAATGAT GGTACCAGAG 
CTTCAGATGC AGCCAACACA AGGGAACAAC CAAGGAATTT CTGCTTTCAA CGGGGTGCCT 
ACTGCTTTTG CTAATCAGAC AACTACTTCA CCTGTGCAGA CATATCCAGG CCATCCCCAG 
CAGCAGCATC AAATGCCTGC ACAACAGTCC AATATGCTTA GCAATCCTCA TCATCCTAAT 
CTCCGTGGCC CCAATCAGGC TACAGGTTCA CAGCATCCAG CATATGCTAT GCACCTGCCT 
AAAGGAAGGC AAATGCAGCA GCGGCTTCTC CAGCAGCAGC AGCAGCAGCA GCAGCACTTT 
TCTGCATCTA GTGCTTTGAT GCCCCATGTC CAACATCAAT CCCAACTTCC CATATCATCG 
TCTATGCAAA ATAGTTCTCA AATCTCACCA CCATCTGCAT CGCAGCCGGT GTCACTACCT 
GCTATAACTC CCCCTTCCCC AATGACTCCC ATTTCAATGC AGCAGCAACA GCAGCAGAAA 
CACAACTTGC CACATCATGC GGTCAGTCGG AGCCCCCAAA GTGGTTCCAG TGGGTTGACC 
AATCAGATGG GAAAGCAGCG ACAACGGCAA CCACAGCAGT TTCAACAATC TGGCAGACAT 
CACCCTCAAC AACGACAACA CTCACAGTCT CCACAGCAGG CTAAACTTTT GAAGGGAATG 
GGGAGAGGGA ACATGGTGGT GCACCAGAAC CTTCCCAATG ATCACTCTCC TTTGAATGGC 
CTTTCTGTGC CTCCAGGAAA CCATGGTGTA GAGAAAGGAG AGCAAATCAT GCACTTGATG 
CAGGGTCAAG GCTTGTATTC TGGGACTGGG TTGAGTCCAA TTCATACATC TAAACCATTG 
GGTCCTTCAC AATCTCCAAA CCATTCTCAG CCACAGCAAA AGCTATATTC GGGCCCTACA 
ACCCCTTCAT CAAAGCCACT CCAGCAAATG CCTTCTCATT TGGAAAGTAG TACTCAAGGC 
CAGGTCCAAC CAGTACCCTC TGGTCAAACA CTAACAGCTA CTCACCAGAA CACTCCAGTT 
ATGGTTCCCA GTCATCAGCA TCTTCAACAA CATCCACAGC CACATCAGAA GCAGGTTAGT 
CAACCTCAAC CAACTGTTCA GAGAATGCTT CAACAGAGTC GTCTGTTGAA TTCTGATCTG 
CCAACAAAGC CTCAAACTGA TCAGGGCCAT GCTGATCAGC AAACTTCAAA CAACATCTCC 
CAGACAGGAA CAAGTACATC CACAGGGATG CCTTTGGCCT GTAATGATAC ATCTAATGTG 
GCACCAGTTG TTTCTTCTGT TTCTGAGATG CAGTGGAAAT CATCAGAACC ATCCTTGCAT 
GATTCTGGCA TGGCAGATAC AGCTTCTAAG GTAGGTCCTA TTGGTAGCCC CCCCCTTACG 
AGTGCAGCTG GGAGTGAGCA AGTGGTGCCT GTCAGCCAAG GTTCAGTCCA CAGGCAGTTA 
TCAGGAGGCT TGCCCCTGCA TTGCCATAAT GGTGGGACAC GGTGGCAGCA CCAACAACCA 
CATCAATCAA CAGCATCGCC TTCCCCATGC CAGCAACTTT TGCAGTCACA AGAGCCACAA 
CTACAGCAAG AACGACAACA ATCACCTCAA CAGCTGCCAC CACAGCAGCC GACTCAGAAG 
CAGACACAGC ATCTTCAATC AGGACAAGGC AATTTATACA TCACGCCAAG CAATTCTGGT 
CTGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7459484 ( XM_024595576.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024451344.1 Populus trichocarpa chromatin modification-related protein EAF1 B (LOC7459484), transcript variant X6, mRNA.

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CloneID	OPa424910
Clone ID Related Accession (Same CDS sequence)	XM_024595576.1
Accession Version	XM_024595576.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	chromatin modification-related protein EAF1 B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037286.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGGAT GTGGTTCAGG ATATGCTCTC CTAGTAAATG CTGAGGTTGA TTCCATGGGT 
GGGGTTGTTG ATGGTGGAGT TGGTATTGCT ACCAAAACCT CTCCGCGGCG AGCAGCAATT 
GAGAAGGCCC AGGTGGAACT TAGACAAGAG TATGATGTTC GTGAGGAACG GAGAAGGGAG 
CTAGAATTTC TTGAGAAAGG CGGCAATCCT TTGGATTTTA AATTTGTAAA TGCAACTTCG 
GTCAGTGTCC AGTCTACTTC ACTCACAGAT CATCACGTTG AACAGTTTGT TACTAGTGAA 
GCAAAAGGCA GTTTTCCACT GACTGCATCA CCTCATGGGG ATTCTGTAGA AAGTAGTGGC 
AGACCAGGGG CTACTCCAGT TTGTGAGCCC AATAGTGCTG ACAATTTTGA TGGTGAAAAT 
GAGTTACTTG AAGTTGAAAG GAAACGTAAA CATCCTAGTA GGAGGAATAA TGTCACTCAA 
TCAGAGCAGT CTTCACAGAT GGATGGAATT CACAATGCCA AGGAATCAGA AGATTCTGCT 
ATTTTTCGGC CATATGCTCG AAGGAATAGG TCCAGACCAA ATCGTGACGG CGCGCGATCA 
AGTTCAACTG ATATTGTTCA GAGTTCAGTT GGCCATGGAT CCTCTTTACC GGCTCGTGGG 
GATGCAAGGG ATGTGAAAGG GTTGGTAACT GAAACAGACG ATCATAAAGC CCAGATTATT 
ACTTCCATCT CTAATCCAAA GTCCACCACT TCAAACGGTG ACCTGTTTTT TCAGATTGAC 
ACTTCCAATA CACAGTCAAA CACGGAGCTT GATTGTGTGC AGGCTCTGAA AACAGTAGTC 
AACCTGCCTG ATGATAGGTT GGATGTTACA GAGTCCATTG TCTTGAGGGA TAACCAACAT 
GACCAGCCTT CAGAAGCTGA TGCTGAGAAA GCACCCAATG ATATTGCTTC CAGGGAATGC 
GATCATGGTG GAGGAAAAGA GCTGGTTATT TCAGCTGGTC CTGAATGTCC CCCTTGTGCT 
GAATCAGCCA AGACTGAGAA TGAAACTGGT CCTGCTCTGC TCAATGGCTT GGAAAAAGAT 
GGAAATGAAG GTCAAAATGG TAATGCAGCA ATGGGAACGG AACGATTCAA TTCAGAGTCC 
TCTTGCACCC AAAATAGCTT AAGCCTGGAT GCAAACAATG GCTGTGATCC TTGTGATAAT 
CGTAGAAATG ATGACACCAA TGAAATTCTT TTGAAAGAAT CATCAGAATT TGAAGGAACA 
CGAAGTTTAC CATCGGGTAA TATAGGAAAT GAAAAGAAAG AGACTAACTC GATCTCTGCA 
ATCAATGATG GTTCTGTGCA TGAAAACTAC TCTGGCAATG ATTCTACTGT CAAAAATGAG 
GAAGAAAGGA GAACAACTTT TCACTCTCAG GTGAAGTGTA CTAATCTTGA AGGAGTGGAA 
CAAAATGACC ATGTTGCCTC TGAGGCTGAT ACAAAGGCGG GCAACATGTT GGCTGATAGT 
TCTAATTCCA TCAGGGAAAT AATTTACCCC AGTGGTCCCC AGGGCTCTTT GGATCCTCCT 
GTTCAAGAGC TTCCCCAGCC TATTTTGTTA GAGAAAAACT CTTTTGTTGC CACTGATCCT 
CAATCTTGTT CGAATACACA TGTTAAAGTG GTAGACAAGT CTCATGAAGA TTCTATTTTG 
GAAGAGGCAC GGGTTATAGA GGCTAAGCGC AAGAGGATTG CAGAATTATC TGTTGCATCT 
GTGCATTCAG AGAATCGCCG GAGATCTCAC TGGGATTTTG TCCTTGAAGA AATGGCATGG 
CTGGCAAATG ATGTTGCACA GGAGCGTCTC TGGAAGATGA CTGCTGCTGC TCAAATATGT 
CGACGAATTG CTTTTACTTC TCGATTGAGA GTTGAAGAGC AGAATCATCA TTTGAAGCTA 
AAAAACGTCG CTTACAGTCT TGCTAAGGCT GTCATGCAGT TCTGGCATTC AGCAAAGGTG 
TATCTAAGTA ACAATTGTCA CAGTGTTGGT TCAAAGAATG GGAAGCATGA GGTGGGGATG 
TTTGTTGGGA ATGAATTTTC TGTCAACAAG TTTGGAGATA TTGATAAGGT GGCATGCAAA 
GAGTTAGAGA AACAAAATCC TGCCAAAAAC ATTGCGCATT CCATTCATGG ATATGCTGTA 
AGGTTTTTGA AATATAACAG TTCTCCATTT CCTTCCTTTC AAGCAGAAGC ACCAGCAACT 
CCTGATAGAA TAGCTGATTT GGGCATTGTG GACACTTCAT GGGATGATCG CCTTACTGAA 
GAAAGCCTGT TCTACGCAGT TCCCTCAGGT GCCATGGCAA TGTACAGATT GTCCATTGAA 
TCTCATATTG CGCAGAGTGA GAAAACTCGC AGTAGCATGC AGGAGGAAGT CGATACATCC 
ATGTATGACA CTCCAGCAGA TTTTGGATAT CATGATACTG CTGCATACGA TGAGGAGGAA 
GGAGAAACAA GTGCTTACTA TATGCATGGA GTCTTTGAAG GTAGCAAGTC AGCAAAACAT 
GACCAAAAGA AACGGAAAAG CTTAACAAAG TCACCCTCTG CAAGATCTTA TGATCTGGGA 
ACTGATTCAC CTTATGGACA CTGCACCACT GGGCCACAAC AAAATGTATT GATGGGGAAA 
AGACCTGCTA GTAATCTTAA TGCTGGCTCA ATCCCAACAA AACGCATGCG TACTGCTTCT 
AGACAGAGGT TTACAAGTCC TTTTACTGCT GGAACTGCTG GGGTTTTACT CCAGGCTCCA 
GTGAAGACAG ATGCATCGAG TGGAGATACC AATTCTTTTC AGGATGATCA GAGTATTTTG 
CATGGTGGGT CACAAATTCA GAAAAGTGTG GAGGTTGAGT CCGCTGCGCA TTTCGAAAGG 
CAGTTACCAT ATGACTACGC AGAGACATCA ACAAAACCGA AGAAGAAGAA GAAGGCAAAG 
CACCTGGGTT CTGCTTATGA GCAGGGGTGG CAGTTGGATT CTACTGGTCA TAATGAACAG 
AGGGATAATT TCAAAAAGAG ATCGGAGAGT CATCATTTGG ACTCTAATGG AACCAGTGGT 
TTATATGGGC AACATACCAC AAAGAAGCCA AAGATATCTA AGCAATTGCT AGATAATACT 
TTTGACAACA TGGTTCAAAT GACCGGGTCC ATCCCATCTC CAGCTGCTTC CCAGATGAGT 
AATATGTCAA ACACAAATAG ATTCATCAAA TTGATTGGTG GCAGGGAACG AGGCAGAAAA 
AATAAATCAA TGAAGATGTC TGTTGGGCAG CCAGGTTCTG GAAGTCCCTG GTCACTTTTT 
GAAGACCAGG CTCTTGTTGT TCTTGTGCAT GACATGGGTC CTAATTGGGA GCTTATAAGC 
GATGCTATCA ACAGCACTGC TCAATTCAAG TGTATATTCC GCAAGCCTAA AGAATGCAAA 
GATCGTCACA AAATCTTAAT GGATAAGGGT GCTGGTGATG GGGCTGATAG TGCTGAGGAT 
TCAGGATCTT CTCAATCCTA CCCATCAACT TTGCCTGGCA TTCCAAAGCA GGGCAGTGCC 
AGACAGTTGT TTCAACATTT GCAAGGGCCG ATGCAAGAGG ATACCCTGAA GTCTCATTTT 
GAGAAAATCA TCATTATTGG GAAGAAACAT CATTACAAAA GGAGTCAGAA TGAAAACCAG 
GATCCCAAGC AAATAGCAGC AACTCACAAT TCTCATTTTA TTGCTCTTTC CCAAGTTTGC 
CCAAACAACT TAAATGGAGG TGTCCTAACG CCCCTTGATC TCTGTGATTC AAGCACTTCA 
AATCCAGATG TTCTTCCCAA TGTGTATCAA GGTTCTCATG CTAGCAATTT GGTAATGCCA 
AACCAAGGTG CTGTTGCATC AACGCTTCCC ACTTCTGGTG CAATCTCTTC TCTGCAAGGA 
TCTTCTGGCG TGGTTCTTGG CAATAACTCA TCATCACCAT CTGGTCCCCT CAATGCACCT 
CATAGAGATG GTAGATACAA TGTTCCAAGG ACATCTTTAC CAGTTGACGA GCATCAAAGA 
ATGCAACCCT ATCAAATGTT ACCTAGTAGA AACTTGCAGC AGTCTAACAT GTCGGTTTCT 
GGGGCTGTCT CTGGAGCTGA TCGTGGTGTT CGCATGTTAT CGAGTGGAAA TGGTATGGGC 
ATGATGCCAG GGATGAACAG AAGCATGCCA CTGCCACGGT CAGGATTTCA AGGAACAGCC 
TCTTCATCAA TGCTGAATTC TGGAAGTATG CTTTCTAACA ATGTGGTTGG GATGTCAAGC 
CCTGTGAATA TGCACACTGG TTCTGGTCAA GGGAACTTGA TGAGACCTCG CGAGGCTTTG 
CATATGTTGA GGCTTGGCCA TAATCATGAG CATCAAAGAC AAATGATGGT ACCAGAGCTT 
CAGATGCAGC CAACACAAGG GAACAACCAA GGAATTTCTG CTTTCAACGG GGTGCCTACT 
GCTTTTGCTA ATCAGACAAC TACTTCACCT GTGCAGACAT ATCCAGGCCA TCCCCAGCAG 
CAGCATCAAA TGCCTGCACA ACAGTCCAAT ATGCTTAGCA ATCCTCATCA TCCTAATCTC 
CGTGGCCCCA ATCAGGCTAC AGGTTCACAG CATCCAGCAT ATGCTATGCA CCTGCCTAAA 
GGAAGGCAAA TGCAGCAGCG GCTTCTCCAG CAGCAGCAGC AGCAGCAGCA GCACTTTTCT 
GCATCTAGTG CTTTGATGCC CCATGTCCAA CATCAATCCC AACTTCCCAT ATCATCGTCT 
ATGCAAAATA GTTCTCAAAT CTCACCACCA TCTGCATCGC AGCCGGTGTC ACTACCTGCT 
ATAACTCCCC CTTCCCCAAT GACTCCCATT TCAATGCAGC AGCAACAGCA GCAGAAACAC 
AACTTGCCAC ATCATGCGGT CAGTCGGAGC CCCCAAAGTG GTTCCAGTGG GTTGACCAAT 
CAGATGGGAA AGCAGCGACA ACGGCAACCA CAGCAGTTTC AACAATCTGG CAGACATCAC 
CCTCAACAAC GACAACACTC ACAGTCTCCA CAGCAGGCTA AACTTTTGAA GGGAATGGGG 
AGAGGGAACA TGGTGGTGCA CCAGAACCTT CCCAATGATC ACTCTCCTTT GAATGGCCTT 
TCTGTGCCTC CAGGAAACCA TGGTGTAGAG AAAGGAGAGC AAATCATGCA CTTGATGCAG 
GGTCAAGGCT TGTATTCTGG GACTGGGTTG AGTCCAATTC ATACATCTAA ACCATTGGGT 
CCTTCACAAT CTCCAAACCA TTCTCAGCCA CAGCAAAAGC TATATTCGGG CCCTACAACC 
CCTTCATCAA AGCCACTCCA GCAAATGCCT TCTCATTTGG AAAGTAGTAC TCAAGGCCAG 
GTCCAACCAG TACCCTCTGG TCAAACACTA ACAGCTACTC ACCAGAACAC TCCAGTTATG 
GTTCCCAGTC ATCAGCATCT TCAACAACAT CCACAGCCAC ATCAGAAGCA GGTTAGTCAA 
CCTCAACCAA CTGTTCAGAG AATGCTTCAA CAGAGTCGTC TGTTGAATTC TGATCTGCCA 
ACAAAGCCTC AAACTGATCA GGGCCATGCT GATCAGCAAA CTTCAAACAA CATCTCCCAG 
ACAGGAACAA GTACATCCAC AGGGATGCCT TTGGCCTGTA ATGATACATC TAATGTGGCA 
CCAGTTGTTT CTTCTGTTTC TGAGATGCAG TGGAAATCAT CAGAACCATC CTTGCATGAT 
TCTGGCATGG CAGATACAGC TTCTAAGGTA GGTCCTATTG GTAGCCCCCC CCTTACGAGT 
GCAGCTGGGA GTGAGCAAGT GGTGCCTGTC AGCCAAGGTT CAGTCCACAG GCAGTTATCA 
GGAGGCTTGC CCCTGCATTG CCATAATGGT GGGACACGGT GGCAGCACCA ACAACCACAT 
CAATCAACAG CATCGCCTTC CCCATGCCAG CAACTTTTGC AGTCACAAGA GCCACAACTA 
CAGCAAGAAC GACAACAATC ACCTCAACAG CTGCCACCAC AGCAGCCGAC TCAGAAGCAG 
ACACAGCATC TTCAATCAGG ACAAGGCAAT TTATACATCA CGCCAAGCAA TTCTGGTCTG 
GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024451344.1
CDS	401..6466
Translation	MHGCGSGYALLVNAEVDSMGGVVDGGVGIATKTSPRRAAIEKAQVELRQEYDVREERRRELEFLEKGGNPLDFKFVNATSVSVQSTSLTDHHVEQFVTSEAKGSFPLTASPHGDSVESSGRPGATPVCEPNSADNFDGENELLEVERKRKHPSRRNNVTQSEQSSQMDGIHNAKESEDSAIFRPYARRNRSRPNRDGARSSSTDIVQSSVGHGSSLPARGDARDVKGLVTETDDHKAQIITSISNPKSTTSNGDLFFQIDTSNTQSNTELDCVQALKTVVNLPDDRLDVTESIVLRDNQHDQPSEADAEKAPNDIASRECDHGGGKELVISAGPECPPCAESAKTENETGPALLNGLEKDGNEGQNGNAAMGTERFNSESSCTQNSLSLDANNGCDPCDNRRNDDTNEILLKESSEFEGTRSLPSGNIGNEKKETNSISAINDGSVHENYSGNDSTVKNEEERRTTFHSQVKCTNLEGVEQNDHVASEADTKAGNMLADSSNSIREIIYPSGPQGSLDPPVQELPQPILLEKNSFVATDPQSCSNTHVKVVDKSHEDSILEEARVIEAKRKRIAELSVASVHSENRRRSHWDFVLEEMAWLANDVAQERLWKMTAAAQICRRIAFTSRLRVEEQNHHLKLKNVAYSLAKAVMQFWHSAKVYLSNNCHSVGSKNGKHEVGMFVGNEFSVNKFGDIDKVACKELEKQNPAKNIAHSIHGYAVRFLKYNSSPFPSFQAEAPATPDRIADLGIVDTSWDDRLTEESLFYAVPSGAMAMYRLSIESHIAQSEKTRSSMQEEVDTSMYDTPADFGYHDTAAYDEEEGETSAYYMHGVFEGSKSAKHDQKKRKSLTKSPSARSYDLGTDSPYGHCTTGPQQNVLMGKRPASNLNAGSIPTKRMRTASRQRFTSPFTAGTAGVLLQAPVKTDASSGDTNSFQDDQSILHGGSQIQKSVEVESAAHFERQLPYDYAETSTKPKKKKKAKHLGSAYEQGWQLDSTGHNEQRDNFKKRSESHHLDSNGTSGLYGQHTTKKPKISKQLLDNTFDNMVQMTGSIPSPAASQMSNMSNTNRFIKLIGGRERGRKNKSMKMSVGQPGSGSPWSLFEDQALVVLVHDMGPNWELISDAINSTAQFKCIFRKPKECKDRHKILMDKGAGDGADSAEDSGSSQSYPSTLPGIPKQGSARQLFQHLQGPMQEDTLKSHFEKIIIIGKKHHYKRSQNENQDPKQIAATHNSHFIALSQVCPNNLNGGVLTPLDLCDSSTSNPDVLPNVYQGSHASNLVMPNQGAVASTLPTSGAISSLQGSSGVVLGNNSSSPSGPLNAPHRDGRYNVPRTSLPVDEHQRMQPYQMLPSRNLQQSNMSVSGAVSGADRGVRMLSSGNGMGMMPGMNRSMPLPRSGFQGTASSSMLNSGSMLSNNVVGMSSPVNMHTGSGQGNLMRPREALHMLRLGHNHEHQRQMMVPELQMQPTQGNNQGISAFNGVPTAFANQTTTSPVQTYPGHPQQQHQMPAQQSNMLSNPHHPNLRGPNQATGSQHPAYAMHLPKGRQMQQRLLQQQQQQQQHFSASSALMPHVQHQSQLPISSSMQNSSQISPPSASQPVSLPAITPPSPMTPISMQQQQQQKHNLPHHAVSRSPQSGSSGLTNQMGKQRQRQPQQFQQSGRHHPQQRQHSQSPQQAKLLKGMGRGNMVVHQNLPNDHSPLNGLSVPPGNHGVEKGEQIMHLMQGQGLYSGTGLSPIHTSKPLGPSQSPNHSQPQQKLYSGPTTPSSKPLQQMPSHLESSTQGQVQPVPSGQTLTATHQNTPVMVPSHQHLQQHPQPHQKQVSQPQPTVQRMLQQSRLLNSDLPTKPQTDQGHADQQTSNNISQTGTSTSTGMPLACNDTSNVAPVVSSVSEMQWKSSEPSLHDSGMADTASKVGPIGSPPLTSAAGSEQVVPVSQGSVHRQLSGGLPLHCHNGGTRWQHQQPHQSTASPSPCQQLLQSQEPQLQQERQQSPQQLPPQQPTQKQTQHLQSGQGNLYITPSNSGLE

Target ORF information:

RefSeq Version	XM_024595576.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa chromatin modification-related protein EAF1 B (LOC7459484), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024595576.1

ORF Insert Sequence:

ATGCATGGAT GTGGTTCAGG ATATGCTCTC CTAGTAAATG CTGAGGTTGA TTCCATGGGT 
GGGGTTGTTG ATGGTGGAGT TGGTATTGCT ACCAAAACCT CTCCGCGGCG AGCAGCAATT 
GAGAAGGCCC AGGTGGAACT TAGACAAGAG TATGATGTTC GTGAGGAACG GAGAAGGGAG 
CTAGAATTTC TTGAGAAAGG CGGCAATCCT TTGGATTTTA AATTTGTAAA TGCAACTTCG 
GTCAGTGTCC AGTCTACTTC ACTCACAGAT CATCACGTTG AACAGTTTGT TACTAGTGAA 
GCAAAAGGCA GTTTTCCACT GACTGCATCA CCTCATGGGG ATTCTGTAGA AAGTAGTGGC 
AGACCAGGGG CTACTCCAGT TTGTGAGCCC AATAGTGCTG ACAATTTTGA TGGTGAAAAT 
GAGTTACTTG AAGTTGAAAG GAAACGTAAA CATCCTAGTA GGAGGAATAA TGTCACTCAA 
TCAGAGCAGT CTTCACAGAT GGATGGAATT CACAATGCCA AGGAATCAGA AGATTCTGCT 
ATTTTTCGGC CATATGCTCG AAGGAATAGG TCCAGACCAA ATCGTGACGG CGCGCGATCA 
AGTTCAACTG ATATTGTTCA GAGTTCAGTT GGCCATGGAT CCTCTTTACC GGCTCGTGGG 
GATGCAAGGG ATGTGAAAGG GTTGGTAACT GAAACAGACG ATCATAAAGC CCAGATTATT 
ACTTCCATCT CTAATCCAAA GTCCACCACT TCAAACGGTG ACCTGTTTTT TCAGATTGAC 
ACTTCCAATA CACAGTCAAA CACGGAGCTT GATTGTGTGC AGGCTCTGAA AACAGTAGTC 
AACCTGCCTG ATGATAGGTT GGATGTTACA GAGTCCATTG TCTTGAGGGA TAACCAACAT 
GACCAGCCTT CAGAAGCTGA TGCTGAGAAA GCACCCAATG ATATTGCTTC CAGGGAATGC 
GATCATGGTG GAGGAAAAGA GCTGGTTATT TCAGCTGGTC CTGAATGTCC CCCTTGTGCT 
GAATCAGCCA AGACTGAGAA TGAAACTGGT CCTGCTCTGC TCAATGGCTT GGAAAAAGAT 
GGAAATGAAG GTCAAAATGG TAATGCAGCA ATGGGAACGG AACGATTCAA TTCAGAGTCC 
TCTTGCACCC AAAATAGCTT AAGCCTGGAT GCAAACAATG GCTGTGATCC TTGTGATAAT 
CGTAGAAATG ATGACACCAA TGAAATTCTT TTGAAAGAAT CATCAGAATT TGAAGGAACA 
CGAAGTTTAC CATCGGGTAA TATAGGAAAT GAAAAGAAAG AGACTAACTC GATCTCTGCA 
ATCAATGATG GTTCTGTGCA TGAAAACTAC TCTGGCAATG ATTCTACTGT CAAAAATGAG 
GAAGAAAGGA GAACAACTTT TCACTCTCAG GTGAAGTGTA CTAATCTTGA AGGAGTGGAA 
CAAAATGACC ATGTTGCCTC TGAGGCTGAT ACAAAGGCGG GCAACATGTT GGCTGATAGT 
TCTAATTCCA TCAGGGAAAT AATTTACCCC AGTGGTCCCC AGGGCTCTTT GGATCCTCCT 
GTTCAAGAGC TTCCCCAGCC TATTTTGTTA GAGAAAAACT CTTTTGTTGC CACTGATCCT 
CAATCTTGTT CGAATACACA TGTTAAAGTG GTAGACAAGT CTCATGAAGA TTCTATTTTG 
GAAGAGGCAC GGGTTATAGA GGCTAAGCGC AAGAGGATTG CAGAATTATC TGTTGCATCT 
GTGCATTCAG AGAATCGCCG GAGATCTCAC TGGGATTTTG TCCTTGAAGA AATGGCATGG 
CTGGCAAATG ATGTTGCACA GGAGCGTCTC TGGAAGATGA CTGCTGCTGC TCAAATATGT 
CGACGAATTG CTTTTACTTC TCGATTGAGA GTTGAAGAGC AGAATCATCA TTTGAAGCTA 
AAAAACGTCG CTTACAGTCT TGCTAAGGCT GTCATGCAGT TCTGGCATTC AGCAAAGGTG 
TATCTAAGTA ACAATTGTCA CAGTGTTGGT TCAAAGAATG GGAAGCATGA GGTGGGGATG 
TTTGTTGGGA ATGAATTTTC TGTCAACAAG TTTGGAGATA TTGATAAGGT GGCATGCAAA 
GAGTTAGAGA AACAAAATCC TGCCAAAAAC ATTGCGCATT CCATTCATGG ATATGCTGTA 
AGGTTTTTGA AATATAACAG TTCTCCATTT CCTTCCTTTC AAGCAGAAGC ACCAGCAACT 
CCTGATAGAA TAGCTGATTT GGGCATTGTG GACACTTCAT GGGATGATCG CCTTACTGAA 
GAAAGCCTGT TCTACGCAGT TCCCTCAGGT GCCATGGCAA TGTACAGATT GTCCATTGAA 
TCTCATATTG CGCAGAGTGA GAAAACTCGC AGTAGCATGC AGGAGGAAGT CGATACATCC 
ATGTATGACA CTCCAGCAGA TTTTGGATAT CATGATACTG CTGCATACGA TGAGGAGGAA 
GGAGAAACAA GTGCTTACTA TATGCATGGA GTCTTTGAAG GTAGCAAGTC AGCAAAACAT 
GACCAAAAGA AACGGAAAAG CTTAACAAAG TCACCCTCTG CAAGATCTTA TGATCTGGGA 
ACTGATTCAC CTTATGGACA CTGCACCACT GGGCCACAAC AAAATGTATT GATGGGGAAA 
AGACCTGCTA GTAATCTTAA TGCTGGCTCA ATCCCAACAA AACGCATGCG TACTGCTTCT 
AGACAGAGGT TTACAAGTCC TTTTACTGCT GGAACTGCTG GGGTTTTACT CCAGGCTCCA 
GTGAAGACAG ATGCATCGAG TGGAGATACC AATTCTTTTC AGGATGATCA GAGTATTTTG 
CATGGTGGGT CACAAATTCA GAAAAGTGTG GAGGTTGAGT CCGCTGCGCA TTTCGAAAGG 
CAGTTACCAT ATGACTACGC AGAGACATCA ACAAAACCGA AGAAGAAGAA GAAGGCAAAG 
CACCTGGGTT CTGCTTATGA GCAGGGGTGG CAGTTGGATT CTACTGGTCA TAATGAACAG 
AGGGATAATT TCAAAAAGAG ATCGGAGAGT CATCATTTGG ACTCTAATGG AACCAGTGGT 
TTATATGGGC AACATACCAC AAAGAAGCCA AAGATATCTA AGCAATTGCT AGATAATACT 
TTTGACAACA TGGTTCAAAT GACCGGGTCC ATCCCATCTC CAGCTGCTTC CCAGATGAGT 
AATATGTCAA ACACAAATAG ATTCATCAAA TTGATTGGTG GCAGGGAACG AGGCAGAAAA 
AATAAATCAA TGAAGATGTC TGTTGGGCAG CCAGGTTCTG GAAGTCCCTG GTCACTTTTT 
GAAGACCAGG CTCTTGTTGT TCTTGTGCAT GACATGGGTC CTAATTGGGA GCTTATAAGC 
GATGCTATCA ACAGCACTGC TCAATTCAAG TGTATATTCC GCAAGCCTAA AGAATGCAAA 
GATCGTCACA AAATCTTAAT GGATAAGGGT GCTGGTGATG GGGCTGATAG TGCTGAGGAT 
TCAGGATCTT CTCAATCCTA CCCATCAACT TTGCCTGGCA TTCCAAAGCA GGGCAGTGCC 
AGACAGTTGT TTCAACATTT GCAAGGGCCG ATGCAAGAGG ATACCCTGAA GTCTCATTTT 
GAGAAAATCA TCATTATTGG GAAGAAACAT CATTACAAAA GGAGTCAGAA TGAAAACCAG 
GATCCCAAGC AAATAGCAGC AACTCACAAT TCTCATTTTA TTGCTCTTTC CCAAGTTTGC 
CCAAACAACT TAAATGGAGG TGTCCTAACG CCCCTTGATC TCTGTGATTC AAGCACTTCA 
AATCCAGATG TTCTTCCCAA TGTGTATCAA GGTTCTCATG CTAGCAATTT GGTAATGCCA 
AACCAAGGTG CTGTTGCATC AACGCTTCCC ACTTCTGGTG CAATCTCTTC TCTGCAAGGA 
TCTTCTGGCG TGGTTCTTGG CAATAACTCA TCATCACCAT CTGGTCCCCT CAATGCACCT 
CATAGAGATG GTAGATACAA TGTTCCAAGG ACATCTTTAC CAGTTGACGA GCATCAAAGA 
ATGCAACCCT ATCAAATGTT ACCTAGTAGA AACTTGCAGC AGTCTAACAT GTCGGTTTCT 
GGGGCTGTCT CTGGAGCTGA TCGTGGTGTT CGCATGTTAT CGAGTGGAAA TGGTATGGGC 
ATGATGCCAG GGATGAACAG AAGCATGCCA CTGCCACGGT CAGGATTTCA AGGAACAGCC 
TCTTCATCAA TGCTGAATTC TGGAAGTATG CTTTCTAACA ATGTGGTTGG GATGTCAAGC 
CCTGTGAATA TGCACACTGG TTCTGGTCAA GGGAACTTGA TGAGACCTCG CGAGGCTTTG 
CATATGTTGA GGCTTGGCCA TAATCATGAG CATCAAAGAC AAATGATGGT ACCAGAGCTT 
CAGATGCAGC CAACACAAGG GAACAACCAA GGAATTTCTG CTTTCAACGG GGTGCCTACT 
GCTTTTGCTA ATCAGACAAC TACTTCACCT GTGCAGACAT ATCCAGGCCA TCCCCAGCAG 
CAGCATCAAA TGCCTGCACA ACAGTCCAAT ATGCTTAGCA ATCCTCATCA TCCTAATCTC 
CGTGGCCCCA ATCAGGCTAC AGGTTCACAG CATCCAGCAT ATGCTATGCA CCTGCCTAAA 
GGAAGGCAAA TGCAGCAGCG GCTTCTCCAG CAGCAGCAGC AGCAGCAGCA GCACTTTTCT 
GCATCTAGTG CTTTGATGCC CCATGTCCAA CATCAATCCC AACTTCCCAT ATCATCGTCT 
ATGCAAAATA GTTCTCAAAT CTCACCACCA TCTGCATCGC AGCCGGTGTC ACTACCTGCT 
ATAACTCCCC CTTCCCCAAT GACTCCCATT TCAATGCAGC AGCAACAGCA GCAGAAACAC 
AACTTGCCAC ATCATGCGGT CAGTCGGAGC CCCCAAAGTG GTTCCAGTGG GTTGACCAAT 
CAGATGGGAA AGCAGCGACA ACGGCAACCA CAGCAGTTTC AACAATCTGG CAGACATCAC 
CCTCAACAAC GACAACACTC ACAGTCTCCA CAGCAGGCTA AACTTTTGAA GGGAATGGGG 
AGAGGGAACA TGGTGGTGCA CCAGAACCTT CCCAATGATC ACTCTCCTTT GAATGGCCTT 
TCTGTGCCTC CAGGAAACCA TGGTGTAGAG AAAGGAGAGC AAATCATGCA CTTGATGCAG 
GGTCAAGGCT TGTATTCTGG GACTGGGTTG AGTCCAATTC ATACATCTAA ACCATTGGGT 
CCTTCACAAT CTCCAAACCA TTCTCAGCCA CAGCAAAAGC TATATTCGGG CCCTACAACC 
CCTTCATCAA AGCCACTCCA GCAAATGCCT TCTCATTTGG AAAGTAGTAC TCAAGGCCAG 
GTCCAACCAG TACCCTCTGG TCAAACACTA ACAGCTACTC ACCAGAACAC TCCAGTTATG 
GTTCCCAGTC ATCAGCATCT TCAACAACAT CCACAGCCAC ATCAGAAGCA GGTTAGTCAA 
CCTCAACCAA CTGTTCAGAG AATGCTTCAA CAGAGTCGTC TGTTGAATTC TGATCTGCCA 
ACAAAGCCTC AAACTGATCA GGGCCATGCT GATCAGCAAA CTTCAAACAA CATCTCCCAG 
ACAGGAACAA GTACATCCAC AGGGATGCCT TTGGCCTGTA ATGATACATC TAATGTGGCA 
CCAGTTGTTT CTTCTGTTTC TGAGATGCAG TGGAAATCAT CAGAACCATC CTTGCATGAT 
TCTGGCATGG CAGATACAGC TTCTAAGGTA GGTCCTATTG GTAGCCCCCC CCTTACGAGT 
GCAGCTGGGA GTGAGCAAGT GGTGCCTGTC AGCCAAGGTT CAGTCCACAG GCAGTTATCA 
GGAGGCTTGC CCCTGCATTG CCATAATGGT GGGACACGGT GGCAGCACCA ACAACCACAT 
CAATCAACAG CATCGCCTTC CCCATGCCAG CAACTTTTGC AGTCACAAGA GCCACAACTA 
CAGCAAGAAC GACAACAATC ACCTCAACAG CTGCCACCAC AGCAGCCGAC TCAGAAGCAG 
ACACAGCATC TTCAATCAGG ACAAGGCAAT TTATACATCA CGCCAAGCAA TTCTGGTCTG 
GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7459484 ( XM_002303006.2 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_002303042.2 Populus trichocarpa hypothetical protein (POPTR_0002s24490g) mRNA, complete cds.

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CloneID	OPa04781
Clone ID Related Accession (Same CDS sequence)	XM_002303006.2
Accession Version	XM_002303006.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-12-30
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_008468). On Dec 16, 2013 this sequence version replaced XM_002303006.1.

ATGCATGGAT GTGGTTCAGG ATATGCTCTC CTAGTAAATG CTGAGGTTGA TTCCATGGGT 
GGGGTTGTTG ATGGTGGAGT TGGTATTGCT ACCAAAACCT CTCCGCGGCG AGCAGCAATT 
GAGAAGGCCC AGGTGGAACT TAGACAAGAG TATGATGTTC GTGAGGAACG GAGAAGGGAG 
CTAGAATTTC TTGAGAAAGG CGGCAATCCT TTGGATTTTA AATTTGTAAA TGCAACTTCG 
GTCAGTGTCC AGTCTACTTC ACTCACAGAT CATCACGTTG AACAGTTTGT TACTAGTGAA 
GCAAAAGGCA GTTTTCCACT GACTGCATCA CCTCATGGGG ATTCTGTAGA AAGTAGTGGC 
AGACCAGGGG CTACTCCAGT TTGTGAGCCC AATAGTGCTG ACAATTTTGA TGGTGAAAAT 
GAGTTACTTG AAGTTGAAAG GAAACGTAAA CATCCTAGTA GGAGGAATAA TGTCACTCAA 
TCAGAGCAGT CTTCACAGAT GGATGGAATT CACAATGCCA AGGAATCAGA AGATTCTGCT 
ATTTTTCGGC CATATGCTCG AAGGAATAGG TCCAGACCAA ATCGTGACGG CGCGCGATCA 
AGTTCAACTG ATATTGTTCA GAGTTCAGTT GGCCATGGAT CGTCTTTACC GGCTCGTGGG 
GATGCAAGGG ATGTGAAAGG GTTGGTAACT GAAACAGACG ATCATAAAGC CCAGATTATT 
ACTTCCATCT CTAATCCAAA GTCCACCACT TCAAACGGTG ACCTGTTTTT TCAGATTGAC 
ACTTCCAATA CACAGTCAAA CACGGAGCTT GATTGTGTGC AGGCTCTGAA AACAGTAGTC 
AACCTGCCTG ATGATAGGTT GGATGTTACA GAGTCCATTG TCTTGAGGGA TAACCAACAT 
GACCAGCCTT CAGAAGCTGA TGCTGAGAAA GCACCCAATG ATATTGCTTC CAGGGAATGC 
GATCATGGTG GAGGAAAAGA GCTGGTTATT TCAGCTGGTC CTGAATGTCC CCCTTGTGCT 
GAATCAGCCA AGACTGAGAA TGAAACTGGT CCTGCTCTGC TCAATGGCTT GGAAAAAGAT 
GGAAATGAAG GTCAAAATGG TAATGCAGCA ATGGGAACGG AACGATTCAA TTCAGAGTCC 
TCTTGCACCC AAAATAGCTT AAGCCTGGAT GCAAACAATG GCTGTGATCC TTGTGATAAT 
CGTAGAAATG ATGACACCAA TGAAATTCTT TTGAAAGAAT CATCAGAATT TGAAGGAACA 
CGAAGTTTAC CATCGGGTAA TATAGGAAAT GAAAAGAAAG AGACTAACTC TATCTCTGCA 
ATCAATGATG GTTCTGTGCA TGAAAACTAC TCTGGCAATG ATTCTACTGT CAAAAATGAG 
GAAGAAAGGA GAACAACTTT TCACTCTCTG GTGAAGTGTA CTAATCTTGA AGGAGTGGAA 
CAAAATGACC ATGTTGCCTC TGAGGCTGAT ACAAAGGCGG GCAACATGTT GGCTGATAGT 
TCTAATTCCA TCAGGGAAAT AATTTACCCC AGTGGTCCCC AGGGCTCTTT GGATCCTCCT 
GTTCAAGAGC TTCCCCAGCC TATTTTGTTA GAGAAAAACT CTTTTGTTGC CACTGATCCT 
CAATCTTGTT CGAATACACA TGTTAAAGTG GTAGACAAGT CTCATGAAGA TTCTATTTTG 
GAAGAGGCAC GGGTTATAGA GGCTAAGCGC AAGAGGATTG CAGAATTATC TGTTGCATCT 
GTGCATTCAG AGAATCGCCG GAGATCTCAC TGGGATTTTG TCCTTGAAGA AATGGCATGG 
CTGGCAAATG ATGTTGCACA GGAGCGTCTC TGGAAGATGA CTGCTGCTGC TCAAATATGT 
CGACGAATTG CTTTTACTTC TCGATTGAGA GTTGAAGAGC AGAATCATCA TTTGAAGCTA 
AAAAACGTCG CTTACAGTCT TGCTAAGGCT GTCATGCAGT TCTGGCATTC AGCAAAGGTG 
TATCTAAGTA ACAATTGTCA CAGTGTTGGT TCAAAGAATG GGAAGCATGA GGTGGGGATG 
TTTGTTGGGA ATGAATTTTC TGTCAACAAG TTTGGAGATA TTGATAAGGA GCAGGTGGCA 
TGCAAAGAGT TAGAGAAACA AAATCGTGCC AAAAACATTG CGCATTCCAT TCATGGATAT 
GCTGTAAGGT TTTTGAAATA TAACAGTTCT CCATTTCCTT CCTTTCAAGC AGAAGCACCA 
GCAACTCCTG ATAGAATAGC TGATTTGGGC ATTGTGGACA CTTCATGGGA TGATCGCCTT 
ACTGAAGAAA GCCTGTTCTA CGCAGTTCCC TCAGGTGCCA TGGCAATGTA CAGATTGTCC 
ATTGAATCTC ATATTGCGCA GAGTGAGAAA ACTCGCAGTA GCATGCAGGA GGAAGTCGAT 
ACATCCATGT ATGACACTCC AGCAGATTTT GGATATCATG ATACTGCTGC ATACGATGAG 
GAGGAAGGAG AAACAAGTGC TTACTATATG CATGGAGTCT TTGAAGGTAG CAAGTCAGCA 
AAACATGACC AAAAGAAACG GAAAAGCTTA ACAAAGTCAC CCTCTGCAAG ATCTTATGAT 
CTGGGAACTG ATTCACCTTA TGGACACTGC ACCACTGGGC CACAACAAAA TGTATTGATG 
GGGAAAAGAC CTGCTAGTAA TCTTAATGCT GGCTCAATCC CAACAAAACG CATGCGTACT 
GCTTCTAGAC AGAGGTTTAC AAGTCCTTTT ACTGCTGGAA CTGCTGGGGT TTTACTCCAG 
GCTCCAGTGA AGACAGATGC ATCGAGTGGA GATACCAATT CTTTTCAGGA TGATCAGAGT 
ATTTTGCATG GTGGGTCACA AATTCAGAAA AGTGTGGAGG TTGAGTCCGC TGCGCATTTC 
GAAAGGCAGT TACCATATGA CTACGCAGAG ACATCAACAA AACCGAAGAA GAAGAAGAAG 
GCAAAGCACC TGGGTTCTGC TTATGAGCAG GGGTGGCAGT TGGATTCTAC TGGTCATAAT 
GAACAGAGGG ATAATTTCAA AAAGAGATCG GAGAGTCATC ATTTGGACTC TAATGGAACC 
AGTGGTTTAT ATGGGCAACA TACCACAAAG AAGCCAAAGA TATCTAAGCA ATTGCTAGAT 
AATACTTTTG ACAACATGGT TCAAATGACC GGGTCCATCC CATCTCCAGC TGCTTCCCAG 
ATGAGTAATA TGTCAAACAC AAATAGATTC ATCAAATTGA TTGGTGGCAG GGAACGAGGC 
AGAAAAAATA AATCAATGAA GATGTCTGTT GGGCAGCCAG GTTCTGGAAG TCCCTGGTCA 
CTTTTTGAAG ACCAGGCTCT TGTTGTTCTT GTGCATGACA TGGGTCCTAA TTGGGAGCTT 
ATAAGCGATG CTATCAACAG CACTGCTCAA TTCAAGTGTA TATTCCGCAA GCCTAAAGAA 
TGCAAAGATC GTCACAAAAT CTTAATGGAT AAGGGTGCTG GTGATGGGGC TGATAGTGCT 
GAGGATTCAG GATCTTCTCA ATCCTACCCA TCAACTTTGC CTGGCATTCC AAAGGGCAGT 
GCCAGACAGT TGTTTCAACA TTTGCAAGGG CCGATGCAAG AGGATACCCT GAAGTCTCAT 
TTTGAGAAAA TCATCATTAT TGGGAAGAAA CATCATTACA AAAGGAGTCA GAATGAAAAC 
CAGGATCCCA AGCAAATAGC AGCAACTCAC AATTCTCATT TTATTGCTCT TTCCCAAGTT 
TGCCCAAACA ACTTAAATGG AGGTGTCCTA ACGCCCCTTG ATCTCTGTGA TTCAAGCACT 
TCAAATCCAG ATGTTCTTCC CATTGTGTAT CAAGGTTCTC ATGCTAGCAA TTTGGTAATG 
CCAAACCAAG GTGCTGTTGC ATCAACGCTT CCCACTTCTG GTGCAATCTC TTCTCTGCAA 
GGATCTTCTG GCGTGGTTCT TGGCAATAAC TCATCATCAC CATCTGGTCC CCTCAATGCA 
CCTCATAGAG ATGGTAGATA CAATGTTCCA AGGACATCTT TACCAGTTGA CGAGCATCAA 
AGAATGCAAC CCTATCAAAT GTTACCTAGT AGAAACTTGC AGCAGTCTAA CATGTCGGTT 
TCTGGGGCTG TCTCTGGAGC TGATCGTGGT GTTCGCATGT TATCGAGTGG AAATGGTATG 
GGCATGATGC CAGGGATGAA CAGAAGCATG CCACTGCCAC GGTCAGGATT TCAAGGAACA 
GCCTCTTCAT CAATGCTGAA TTCTGGAAGT ATGCTTTCTA ACAATGTGGT TGGGATGCCA 
AGCCCTGTGA ATATGCACAC TGGTTCTGGT CAAGGGAACT TGATGAGACC TCGCGAGGCT 
TTGCATATGT TGAGGCTTGG CCATAATCAT GAGCATCAAA GACAAATGAT GGTACCAGAG 
CTTCAGATGC AGCCAACACA AGGGAACAAC CAAGGAATTT CTGCTTTCAA CGGGGTGCCT 
ACTGCTTTTG CTAATCAGAC AACTACTTCA CCTGTGCAGA CATATCCAGG CCATCCCCAG 
CAGCAGCATC AAATGCCTGC ACAACAGTCC AATATGCTTA GCAATCCTCA TCATCCTAAT 
CTCCGTGGCC CCAATCAGGC TACAGCAGCG GCTTCTCCAG CAGCAGCAGC AGCAGCAGCA 
GCACTTTTCT GCATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002303042.2
CDS	119..4756
Translation	MHGCGSGYALLVNAEVDSMGGVVDGGVGIATKTSPRRAAIEKAQVELRQEYDVREERRRELEFLEKGGNPLDFKFVNATSVSVQSTSLTDHHVEQFVTSEAKGSFPLTASPHGDSVESSGRPGATPVCEPNSADNFDGENELLEVERKRKHPSRRNNVTQSEQSSQMDGIHNAKESEDSAIFRPYARRNRSRPNRDGARSSSTDIVQSSVGHGSSLPARGDARDVKGLVTETDDHKAQIITSISNPKSTTSNGDLFFQIDTSNTQSNTELDCVQALKTVVNLPDDRLDVTESIVLRDNQHDQPSEADAEKAPNDIASRECDHGGGKELVISAGPECPPCAESAKTENETGPALLNGLEKDGNEGQNGNAAMGTERFNSESSCTQNSLSLDANNGCDPCDNRRNDDTNEILLKESSEFEGTRSLPSGNIGNEKKETNSISAINDGSVHENYSGNDSTVKNEEERRTTFHSLVKCTNLEGVEQNDHVASEADTKAGNMLADSSNSIREIIYPSGPQGSLDPPVQELPQPILLEKNSFVATDPQSCSNTHVKVVDKSHEDSILEEARVIEAKRKRIAELSVASVHSENRRRSHWDFVLEEMAWLANDVAQERLWKMTAAAQICRRIAFTSRLRVEEQNHHLKLKNVAYSLAKAVMQFWHSAKVYLSNNCHSVGSKNGKHEVGMFVGNEFSVNKFGDIDKEQVACKELEKQNRAKNIAHSIHGYAVRFLKYNSSPFPSFQAEAPATPDRIADLGIVDTSWDDRLTEESLFYAVPSGAMAMYRLSIESHIAQSEKTRSSMQEEVDTSMYDTPADFGYHDTAAYDEEEGETSAYYMHGVFEGSKSAKHDQKKRKSLTKSPSARSYDLGTDSPYGHCTTGPQQNVLMGKRPASNLNAGSIPTKRMRTASRQRFTSPFTAGTAGVLLQAPVKTDASSGDTNSFQDDQSILHGGSQIQKSVEVESAAHFERQLPYDYAETSTKPKKKKKAKHLGSAYEQGWQLDSTGHNEQRDNFKKRSESHHLDSNGTSGLYGQHTTKKPKISKQLLDNTFDNMVQMTGSIPSPAASQMSNMSNTNRFIKLIGGRERGRKNKSMKMSVGQPGSGSPWSLFEDQALVVLVHDMGPNWELISDAINSTAQFKCIFRKPKECKDRHKILMDKGAGDGADSAEDSGSSQSYPSTLPGIPKGSARQLFQHLQGPMQEDTLKSHFEKIIIIGKKHHYKRSQNENQDPKQIAATHNSHFIALSQVCPNNLNGGVLTPLDLCDSSTSNPDVLPIVYQGSHASNLVMPNQGAVASTLPTSGAISSLQGSSGVVLGNNSSSPSGPLNAPHRDGRYNVPRTSLPVDEHQRMQPYQMLPSRNLQQSNMSVSGAVSGADRGVRMLSSGNGMGMMPGMNRSMPLPRSGFQGTASSSMLNSGSMLSNNVVGMPSPVNMHTGSGQGNLMRPREALHMLRLGHNHEHQRQMMVPELQMQPTQGNNQGISAFNGVPTAFANQTTTSPVQTYPGHPQQQHQMPAQQSNMLSNPHHPNLRGPNQATAAASPAAAAAAAALFCI

Target ORF information:

RefSeq Version	XM_002303006.2
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Definition	Populus trichocarpa hypothetical protein (POPTR_0002s24490g) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002303006.2

ORF Insert Sequence:

ATGCATGGAT GTGGTTCAGG ATATGCTCTC CTAGTAAATG CTGAGGTTGA TTCCATGGGT 
GGGGTTGTTG ATGGTGGAGT TGGTATTGCT ACCAAAACCT CTCCGCGGCG AGCAGCAATT 
GAGAAGGCCC AGGTGGAACT TAGACAAGAG TATGATGTTC GTGAGGAACG GAGAAGGGAG 
CTAGAATTTC TTGAGAAAGG CGGCAATCCT TTGGATTTTA AATTTGTAAA TGCAACTTCG 
GTCAGTGTCC AGTCTACTTC ACTCACAGAT CATCACGTTG AACAGTTTGT TACTAGTGAA 
GCAAAAGGCA GTTTTCCACT GACTGCATCA CCTCATGGGG ATTCTGTAGA AAGTAGTGGC 
AGACCAGGGG CTACTCCAGT TTGTGAGCCC AATAGTGCTG ACAATTTTGA TGGTGAAAAT 
GAGTTACTTG AAGTTGAAAG GAAACGTAAA CATCCTAGTA GGAGGAATAA TGTCACTCAA 
TCAGAGCAGT CTTCACAGAT GGATGGAATT CACAATGCCA AGGAATCAGA AGATTCTGCT 
ATTTTTCGGC CATATGCTCG AAGGAATAGG TCCAGACCAA ATCGTGACGG CGCGCGATCA 
AGTTCAACTG ATATTGTTCA GAGTTCAGTT GGCCATGGAT CGTCTTTACC GGCTCGTGGG 
GATGCAAGGG ATGTGAAAGG GTTGGTAACT GAAACAGACG ATCATAAAGC CCAGATTATT 
ACTTCCATCT CTAATCCAAA GTCCACCACT TCAAACGGTG ACCTGTTTTT TCAGATTGAC 
ACTTCCAATA CACAGTCAAA CACGGAGCTT GATTGTGTGC AGGCTCTGAA AACAGTAGTC 
AACCTGCCTG ATGATAGGTT GGATGTTACA GAGTCCATTG TCTTGAGGGA TAACCAACAT 
GACCAGCCTT CAGAAGCTGA TGCTGAGAAA GCACCCAATG ATATTGCTTC CAGGGAATGC 
GATCATGGTG GAGGAAAAGA GCTGGTTATT TCAGCTGGTC CTGAATGTCC CCCTTGTGCT 
GAATCAGCCA AGACTGAGAA TGAAACTGGT CCTGCTCTGC TCAATGGCTT GGAAAAAGAT 
GGAAATGAAG GTCAAAATGG TAATGCAGCA ATGGGAACGG AACGATTCAA TTCAGAGTCC 
TCTTGCACCC AAAATAGCTT AAGCCTGGAT GCAAACAATG GCTGTGATCC TTGTGATAAT 
CGTAGAAATG ATGACACCAA TGAAATTCTT TTGAAAGAAT CATCAGAATT TGAAGGAACA 
CGAAGTTTAC CATCGGGTAA TATAGGAAAT GAAAAGAAAG AGACTAACTC TATCTCTGCA 
ATCAATGATG GTTCTGTGCA TGAAAACTAC TCTGGCAATG ATTCTACTGT CAAAAATGAG 
GAAGAAAGGA GAACAACTTT TCACTCTCTG GTGAAGTGTA CTAATCTTGA AGGAGTGGAA 
CAAAATGACC ATGTTGCCTC TGAGGCTGAT ACAAAGGCGG GCAACATGTT GGCTGATAGT 
TCTAATTCCA TCAGGGAAAT AATTTACCCC AGTGGTCCCC AGGGCTCTTT GGATCCTCCT 
GTTCAAGAGC TTCCCCAGCC TATTTTGTTA GAGAAAAACT CTTTTGTTGC CACTGATCCT 
CAATCTTGTT CGAATACACA TGTTAAAGTG GTAGACAAGT CTCATGAAGA TTCTATTTTG 
GAAGAGGCAC GGGTTATAGA GGCTAAGCGC AAGAGGATTG CAGAATTATC TGTTGCATCT 
GTGCATTCAG AGAATCGCCG GAGATCTCAC TGGGATTTTG TCCTTGAAGA AATGGCATGG 
CTGGCAAATG ATGTTGCACA GGAGCGTCTC TGGAAGATGA CTGCTGCTGC TCAAATATGT 
CGACGAATTG CTTTTACTTC TCGATTGAGA GTTGAAGAGC AGAATCATCA TTTGAAGCTA 
AAAAACGTCG CTTACAGTCT TGCTAAGGCT GTCATGCAGT TCTGGCATTC AGCAAAGGTG 
TATCTAAGTA ACAATTGTCA CAGTGTTGGT TCAAAGAATG GGAAGCATGA GGTGGGGATG 
TTTGTTGGGA ATGAATTTTC TGTCAACAAG TTTGGAGATA TTGATAAGGA GCAGGTGGCA 
TGCAAAGAGT TAGAGAAACA AAATCGTGCC AAAAACATTG CGCATTCCAT TCATGGATAT 
GCTGTAAGGT TTTTGAAATA TAACAGTTCT CCATTTCCTT CCTTTCAAGC AGAAGCACCA 
GCAACTCCTG ATAGAATAGC TGATTTGGGC ATTGTGGACA CTTCATGGGA TGATCGCCTT 
ACTGAAGAAA GCCTGTTCTA CGCAGTTCCC TCAGGTGCCA TGGCAATGTA CAGATTGTCC 
ATTGAATCTC ATATTGCGCA GAGTGAGAAA ACTCGCAGTA GCATGCAGGA GGAAGTCGAT 
ACATCCATGT ATGACACTCC AGCAGATTTT GGATATCATG ATACTGCTGC ATACGATGAG 
GAGGAAGGAG AAACAAGTGC TTACTATATG CATGGAGTCT TTGAAGGTAG CAAGTCAGCA 
AAACATGACC AAAAGAAACG GAAAAGCTTA ACAAAGTCAC CCTCTGCAAG ATCTTATGAT 
CTGGGAACTG ATTCACCTTA TGGACACTGC ACCACTGGGC CACAACAAAA TGTATTGATG 
GGGAAAAGAC CTGCTAGTAA TCTTAATGCT GGCTCAATCC CAACAAAACG CATGCGTACT 
GCTTCTAGAC AGAGGTTTAC AAGTCCTTTT ACTGCTGGAA CTGCTGGGGT TTTACTCCAG 
GCTCCAGTGA AGACAGATGC ATCGAGTGGA GATACCAATT CTTTTCAGGA TGATCAGAGT 
ATTTTGCATG GTGGGTCACA AATTCAGAAA AGTGTGGAGG TTGAGTCCGC TGCGCATTTC 
GAAAGGCAGT TACCATATGA CTACGCAGAG ACATCAACAA AACCGAAGAA GAAGAAGAAG 
GCAAAGCACC TGGGTTCTGC TTATGAGCAG GGGTGGCAGT TGGATTCTAC TGGTCATAAT 
GAACAGAGGG ATAATTTCAA AAAGAGATCG GAGAGTCATC ATTTGGACTC TAATGGAACC 
AGTGGTTTAT ATGGGCAACA TACCACAAAG AAGCCAAAGA TATCTAAGCA ATTGCTAGAT 
AATACTTTTG ACAACATGGT TCAAATGACC GGGTCCATCC CATCTCCAGC TGCTTCCCAG 
ATGAGTAATA TGTCAAACAC AAATAGATTC ATCAAATTGA TTGGTGGCAG GGAACGAGGC 
AGAAAAAATA AATCAATGAA GATGTCTGTT GGGCAGCCAG GTTCTGGAAG TCCCTGGTCA 
CTTTTTGAAG ACCAGGCTCT TGTTGTTCTT GTGCATGACA TGGGTCCTAA TTGGGAGCTT 
ATAAGCGATG CTATCAACAG CACTGCTCAA TTCAAGTGTA TATTCCGCAA GCCTAAAGAA 
TGCAAAGATC GTCACAAAAT CTTAATGGAT AAGGGTGCTG GTGATGGGGC TGATAGTGCT 
GAGGATTCAG GATCTTCTCA ATCCTACCCA TCAACTTTGC CTGGCATTCC AAAGGGCAGT 
GCCAGACAGT TGTTTCAACA TTTGCAAGGG CCGATGCAAG AGGATACCCT GAAGTCTCAT 
TTTGAGAAAA TCATCATTAT TGGGAAGAAA CATCATTACA AAAGGAGTCA GAATGAAAAC 
CAGGATCCCA AGCAAATAGC AGCAACTCAC AATTCTCATT TTATTGCTCT TTCCCAAGTT 
TGCCCAAACA ACTTAAATGG AGGTGTCCTA ACGCCCCTTG ATCTCTGTGA TTCAAGCACT 
TCAAATCCAG ATGTTCTTCC CATTGTGTAT CAAGGTTCTC ATGCTAGCAA TTTGGTAATG 
CCAAACCAAG GTGCTGTTGC ATCAACGCTT CCCACTTCTG GTGCAATCTC TTCTCTGCAA 
GGATCTTCTG GCGTGGTTCT TGGCAATAAC TCATCATCAC CATCTGGTCC CCTCAATGCA 
CCTCATAGAG ATGGTAGATA CAATGTTCCA AGGACATCTT TACCAGTTGA CGAGCATCAA 
AGAATGCAAC CCTATCAAAT GTTACCTAGT AGAAACTTGC AGCAGTCTAA CATGTCGGTT 
TCTGGGGCTG TCTCTGGAGC TGATCGTGGT GTTCGCATGT TATCGAGTGG AAATGGTATG 
GGCATGATGC CAGGGATGAA CAGAAGCATG CCACTGCCAC GGTCAGGATT TCAAGGAACA 
GCCTCTTCAT CAATGCTGAA TTCTGGAAGT ATGCTTTCTA ACAATGTGGT TGGGATGCCA 
AGCCCTGTGA ATATGCACAC TGGTTCTGGT CAAGGGAACT TGATGAGACC TCGCGAGGCT 
TTGCATATGT TGAGGCTTGG CCATAATCAT GAGCATCAAA GACAAATGAT GGTACCAGAG 
CTTCAGATGC AGCCAACACA AGGGAACAAC CAAGGAATTT CTGCTTTCAA CGGGGTGCCT 
ACTGCTTTTG CTAATCAGAC AACTACTTCA CCTGTGCAGA CATATCCAGG CCATCCCCAG 
CAGCAGCATC AAATGCCTGC ACAACAGTCC AATATGCTTA GCAATCCTCA TCATCCTAAT 
CTCCGTGGCC CCAATCAGGC TACAGCAGCG GCTTCTCCAG CAGCAGCAGC AGCAGCAGCA 
GCACTTTTCT GCATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)
Tuskan GA,Difazio S,Jansson S,Bohlmann J,Grigoriev I,Hellsten U,Putnam N,Ralph S,Rombauts S,Salamov A,Schein J,Sterck L,Aerts A,Bhalerao RR,Bhalerao RP,Blaudez D,Boerjan W,Brun A,Brunner A,Busov V,Campbell M,Carlson J,Chalot M,Chapman J,Chen GL,Cooper D,Coutinho PM,Couturier J,Covert S,Cronk Q,Cunningham R,Davis J,Degroeve S,D?jardin A,Depamphilis C,Detter J,Dirks B,Dubchak I,Duplessis S,Ehlting J,Ellis B,Gendler K,Goodstein D,Gribskov M,Grimwood J,Groover A,Gunter L,Hamberger B,Heinze B,Helariutta Y,Henrissat B,Holligan D,Holt R,Huang W,Islam-Faridi N,Jones S,Jones-Rhoades M,Jorgensen R,Joshi C,Kangasj?rvi J,Karlsson J,Kelleher C,Kirkpatrick R,Kirst M,Kohler A,Kalluri U,Larimer F,Leebens-Mack J,Lepl? JC,Locascio P,Lou Y,Lucas S,Martin F,Montanini B,Napoli C,Nelson DR,Nelson C,Nieminen K,Nilsson O,Pereda V,Peter G,Philippe R,Pilate G,Poliakov A,Razumovskaya J,Richardson P,Rinaldi C,Ritland K,Rouz? P,Ryaboy D,Schmutz J,Schrader J,Segerman B,Shin H,Siddiqui A,Sterky F,Terry A,Tsai CJ,Uberbacher E,Unneberg P,Vahala J,Wall K,Wessler S,Yang G,Yin T,Douglas C,Marra M,Sandberg G,Van de Peer Y,Rokhsar D

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LOC7459484 cDNA ORF clone, Populus trichocarpa(black cottonwood)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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